From tahseen <@t> brain.net.pk Tue Dec 1 00:48:28 2009 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Tue Dec 1 00:48:39 2009 Subject: [Histonet] dictation systems In-Reply-To: <38A56C4F4630D348A50B3720409270870744FEB3@dhmail.dhorg.org> References: <38A56C4F4630D348A50B3720409270870744FEB3@dhmail.dhorg.org> Message-ID: <7292.202.125.145.178.1259650108.squirrel@brain.net.pk> We have 710 transcription system PHILIPS. M.Tahseen SKMCH&RE Lahore Pakistan Does anyone have any recommendations for a dictation system that can be > used in the pathology lab? > We prefer to continue to use the foot pedals but are willing to look into > other options. > > Thanks for your input > Allison > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From louise.renton <@t> gmail.com Tue Dec 1 03:58:48 2009 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Dec 1 03:58:54 2009 Subject: [Histonet] OT - adoptive t cell therapy Message-ID: Hi all, this is a very OT subject, but does anyone know about the above therapy in regards to adenocarcinoma? (i know that trialls are being done on melanomas presently). i would be grateful for any info -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From akbitting <@t> geisinger.edu Tue Dec 1 07:56:52 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Dec 1 07:57:02 2009 Subject: [Histonet] Tau on the Benchmark XT Message-ID: <4B14DA54.2B7F.00C9.0@geisinger.edu> Good morning, I'm still looking for somebody who is running Tau on their XT. I'm using Biocare's mouse monoclonal antibody with Ultraview Detection on human brain, but I'm not getting any staining. Anyone else having similar problems? ~A Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From hughesk <@t> upstate.edu Tue Dec 1 09:52:33 2009 From: hughesk <@t> upstate.edu (Karen Hughes) Date: Tue Dec 1 09:52:41 2009 Subject: [Histonet] MMA Sections Message-ID: <4B14F5720200000C0079DDFE@gwmta2.upstate.edu> I am currently embeding 2cm sections of adult rat spine in MMA including muscle, bone and spinal cord. I then cut 5um sections using a tungsten carbide D profile knife on a manual microtome and mount the sections on APES coated slides, press them in a vise between tongue depressors, and bake them @ 41'C for a min. of 48hrs. My problem is that when I de-placticize the slides in Xylene (4 changes, 2x5min, 2x10min) the spinal cord falls off the slide, while the bone stays secure. I have also tried PolyLysine coated slides, SuperFrost Plus, and Probe On Slides. All with the same results. Does anyone have any experience with this? Any and all Suggestions would be greatly appreciated. Karen Karen S. Hughes Research Support Specialist SUNY Upstate Medical University Phone (315)464-8585 hughesk@upstate.edu From kreaser <@t> vet.upenn.edu Tue Dec 1 10:04:38 2009 From: kreaser <@t> vet.upenn.edu (Karie Reaser) Date: Tue Dec 1 10:04:46 2009 Subject: [Histonet] Bone labelling for Microdamage Message-ID: <232236925.7349751259683478620.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> Is it ok to process cortical bone, embed in MMA, section and grind on the Exakt system. Last do the bone labelling stain? Or do I have to do the bone labelling stain prior to embedding, sectioning and mounting on slides? Any suggestions would be greatly appreciated. -- Karie L Reaser A.S. New Bolton Center University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Research Laboratory 382 W Street Road Kennett Square, PA. 19348 Phone:610-925-6278 Fax:610-925-8120 Email:kreaser@vet.upenn.edu From DKBoyd <@t> chs.net Tue Dec 1 11:01:28 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Tue Dec 1 11:00:54 2009 Subject: [Histonet] Slide Crusher Message-ID: Does anyone have information about glass crushers? We are trying to decide if it would be a justifiable purchase. Thanks Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From ratliffjack <@t> hotmail.com Tue Dec 1 11:36:13 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Dec 1 11:36:29 2009 Subject: [Histonet] Bone labelling for Microdamage In-Reply-To: <232236925.7349751259683478620.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> References: <232236925.7349751259683478620.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> Message-ID: Karie, If you are looking for microdamage or are using basic fuchsin, I have been taught to bulk stain with 1% basic fuchsin in your processing solutions (i.e. 1% BF in 70% EtOH, 1% BF in 80% EtOH, 1% BF in 95% EtOH, and 1% BF in 100% EtOH) and then finish as you would with the remaining steps for your MMA protocol (i.e. xylenes, MMA +DBP, etc.) to achieve a fully polymerized bulk stained specimen. You then cut, grind, and polish as required for use with the EXAKT system. I was told the reason you process with the BF is because the cutting and grinding causes additional microdamage and if you stain first the additional damage will not be stained. If you are talking about labeling bone for calculations of BFR or MAR and the animal did not receive timed injections of calcein, alizarin, tetracycline, or some other fluorescent bone label before necropsy, then you could try doing a 0.6% FA etch for 30 seconds followed with Sanderson's Rapid Bone Stain for 3 minutes, brief tap water rinse, and blot dry. What is the exact specimen you are working with and what do you specifically wish to demonstrate???? Jack On Dec 1, 2009, at 10:04 AM, Karie Reaser wrote: > Is it ok to process cortical bone, embed in MMA, section and grind > on the Exakt system. Last do the bone labelling stain? Or do I have > to do the bone labelling stain prior to embedding, sectioning and > mounting on slides? Any suggestions would be greatly appreciated. > > -- > Karie L Reaser A.S. > New Bolton Center > University of Pennsylvania > School of Veterinary Medicine > Comparative Orthopedic Research Laboratory > 382 W Street Road > Kennett Square, PA. 19348 > Phone:610-925-6278 > Fax:610-925-8120 > Email:kreaser@vet.upenn.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ratliffjack <@t> hotmail.com Tue Dec 1 11:52:07 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Dec 1 11:52:21 2009 Subject: [Histonet] MMA Sections In-Reply-To: <4B14F5720200000C0079DDFE@gwmta2.upstate.edu> References: <4B14F5720200000C0079DDFE@gwmta2.upstate.edu> Message-ID: Karen, How about coating your slides with Haupt's. I have used this solution exclusively for all my undemineralized thin section bone work. Also, I only need to dry my slides overnight using a dense aluminum slide press (available via Dorn and Hart Microedge). Basically, the slide press acts to evenly flatten and "press" the sections to the Haupt's coated slide and also evenly heat all the slides together. Because of it's heat conducting properties, the press acts as a mini-oven within the oven to speed up the drying and section adhesion process. Please feel free to contact me if you have any questions regarding this or any other resin related topic. Regards, Jack Ratliff NSH Hard Tissue Committee Chair On Dec 1, 2009, at 9:52 AM, "Karen Hughes" wrote: > I am currently embeding 2cm sections of adult rat spine in MMA > including > muscle, bone and spinal cord. I then cut 5um sections using a > tungsten > carbide D profile knife on a manual microtome and mount the sections > on APES > coated slides, press them in a vise between tongue depressors, and > bake them > @ 41'C for a min. of 48hrs. My problem is that when I de-placticize > the > slides in Xylene (4 changes, 2x5min, 2x10min) the spinal cord falls > off the > slide, while the bone stays secure. > I have also tried PolyLysine coated slides, SuperFrost Plus, and > Probe On > Slides. All with the same results. > > Does anyone have any experience with this? Any and all Suggestions > would be > greatly appreciated. > > Karen > > Karen S. Hughes > Research Support Specialist > SUNY Upstate Medical University > Phone (315)464-8585 > hughesk@upstate.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Allison_Scott <@t> hchd.tmc.edu Tue Dec 1 12:05:33 2009 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Tue Dec 1 12:05:37 2009 Subject: [Histonet] SWOT Analysis Message-ID: <1872B4A455B7974391609AD8034C79FC8BD659@LBEXCH01.hchd.local> Hello to all in histoland. Have any of you had to do a swot analysis of your lab. I am in the process of doing one for my lab. It makes one think. What are your thoughts on this. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From mward <@t> wfubmc.edu Tue Dec 1 12:15:29 2009 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Dec 1 12:16:11 2009 Subject: [Histonet] tech schedules Message-ID: <61135F0455D33347B5AAE209B903A30429D3526C@EXCHVS2.medctr.ad.wfubmc.edu> I am posting this for my department manager. He is wondering how other labs schedule their techs. In other words, do the techs work fixed times (ie, come in always at 5 am) or do they rotate schedules (ie, come in at 5 am for one month, then 6 am the next month, etc). I will pass along your responses to him. Thanks in advance for any help you can give. Martha Ward Wake Forest University Baptist Medical Center From rjbuesa <@t> yahoo.com Tue Dec 1 12:19:54 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 1 12:19:59 2009 Subject: [Histonet] tech schedules In-Reply-To: <61135F0455D33347B5AAE209B903A30429D3526C@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: <963103.39298.qm@web65711.mail.ac4.yahoo.com> I always rotated all the staff between 1, 2nd and 3rd shifts, and days of the week. All ended working all shifts during the month. They also rotated between tasks (embed, cut, special procedures). Ren? J. --- On Tue, 12/1/09, Martha Ward wrote: From: Martha Ward Subject: [Histonet] tech schedules To: histonet@lists.utsouthwestern.edu Date: Tuesday, December 1, 2009, 1:15 PM I am posting this for my department manager.? He is wondering how other labs schedule their techs.? In other words, do the techs work fixed times (ie, come in always at 5 am) or do they rotate schedules (ie, come in at 5 am for one month, then 6 am the next month, etc).? I will pass along your responses to him.? Thanks in advance for any help you can give. Martha Ward Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kalschev <@t> svm.vetmed.wisc.edu Tue Dec 1 12:23:46 2009 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Tue Dec 1 12:24:46 2009 Subject: [Histonet] Karie - microdamage in bone Message-ID: <00d101ca72b3$6bf94090$c5d76880@vetmed.wisc.edu> Karie, Basic fuchsin works well for microcracks - stain before processing;cutting; grinding. Schedule used will depend in size of bone and species. You want to avoid over-staining. If time allows, check literature in: Journal of Orthopedic Research; Bone; and American Journal of Veterinary Research - 2001 - present, for starters. Feel free to contact me. VK From trathborne <@t> somerset-healthcare.com Tue Dec 1 12:24:55 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Dec 1 12:25:02 2009 Subject: [Histonet] tech schedules In-Reply-To: <963103.39298.qm@web65711.mail.ac4.yahoo.com> Message-ID: I have staff scheduled to arrive at 5:00, 6:00, and 7:30. Everyone has an opportunity do everything. There is a rotation for IHC and other tasks such as changing the processor. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Tuesday, December 01, 2009 1:20 PM To: histonet@lists.utsouthwestern.edu; Martha Ward Subject: Re: [Histonet] tech schedules I always rotated all the staff between 1, 2nd and 3rd shifts, and days of the week. All ended working all shifts during the month. They also rotated between tasks (embed, cut, special procedures). Ren? J. --- On Tue, 12/1/09, Martha Ward wrote: From: Martha Ward Subject: [Histonet] tech schedules To: histonet@lists.utsouthwestern.edu Date: Tuesday, December 1, 2009, 1:15 PM I am posting this for my department manager.? He is wondering how other labs schedule their techs.? In other words, do the techs work fixed times (ie, come in always at 5 am) or do they rotate schedules (ie, come in at 5 am for one month, then 6 am the next month, etc).? I will pass along your responses to him.? Thanks in advance for any help you can give. Martha Ward Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From micropathlabs <@t> yahoo.com Tue Dec 1 12:27:41 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Tue Dec 1 12:27:46 2009 Subject: [Histonet] tech schedules In-Reply-To: <61135F0455D33347B5AAE209B903A30429D3526C@EXCHVS2.medctr.ad.wfubmc.edu> References: <61135F0455D33347B5AAE209B903A30429D3526C@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: <18401.18533.qm@web57802.mail.re3.yahoo.com> We do not rotate schedules here nor?have we at any facility I've worked at over the years?in this area. ? Sheila Haas Laboratory Supervisor Micro Path Laboratories ? ________________________________ From: Martha Ward To: histonet@lists.utsouthwestern.edu Sent: Tue, December 1, 2009 1:15:29 PM Subject: [Histonet] tech schedules I am posting this for my department manager.? He is wondering how other labs schedule their techs.? In other words, do the techs work fixed times (ie, come in always at 5 am) or do they rotate schedules (ie, come in at 5 am for one month, then 6 am the next month, etc).? I will pass along your responses to him.? Thanks in advance for any help you can give. Martha Ward Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Tue Dec 1 12:29:02 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Dec 1 12:29:08 2009 Subject: [Histonet] SWOT Analysis In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD659@LBEXCH01.hchd.local> Message-ID: We have to do one for each new position or vacated position. I think that they are very helpful in justifying the needs of the department. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Scott, Allison D Sent: Tuesday, December 01, 2009 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SWOT Analysis Hello to all in histoland. Have any of you had to do a swot analysis of your lab. I am in the process of doing one for my lab. It makes one think. What are your thoughts on this. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From DKBoyd <@t> chs.net Tue Dec 1 12:41:26 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Tue Dec 1 12:40:51 2009 Subject: [Histonet] tech schedules In-Reply-To: <61135F0455D33347B5AAE209B903A30429D3526C@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: I have one person coming in @ 6am, @6:30, @7 and @ 7:30. The 1st three time positions rotate q 3 days. Duties are assigned according to time ; ie: 6 am embeds, 6:30 cleans blocks and pulls worksheets, 6:30 and 7 am cut while the 6 o'clock person cleans up and starts getting slides out while the other two cut. The 7:30 slot is my regular schedule. I work a bench when someone is off or as workload demands. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net "Martha Ward" Sent by: histonet-bounces@lists.utsouthwestern.edu 12/01/2009 01:17 PM To cc Subject [Histonet] tech schedules I am posting this for my department manager. He is wondering how other labs schedule their techs. In other words, do the techs work fixed times (ie, come in always at 5 am) or do they rotate schedules (ie, come in at 5 am for one month, then 6 am the next month, etc). I will pass along your responses to him. Thanks in advance for any help you can give. Martha Ward Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From talulahgosh <@t> gmail.com Tue Dec 1 12:46:58 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Dec 1 12:47:03 2009 Subject: [Histonet] large coverslips Message-ID: Hello I'm looking for large glass coverslips, (about 1.5 inches square would be great, thickness #1 or 2). I've only been able to find 3 inch coverslips at brainresearchlab.com (from the histonet archive!). Has anyone seen any others out there? Emily ...the thrill of being close to that hidden knowledge. That's the way I feel when I read Nabokov. Encrypted within his words, encoded indecipherably, ambiguously, is the equivalent of the secret of lightning. Something akin to the secret code of higher human consciousness, the DNA, the genome of genius. -Ron Rosenbaum From leiker <@t> buffalo.edu Tue Dec 1 13:12:50 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Tue Dec 1 13:12:58 2009 Subject: [Histonet] Cryostat Poll Message-ID: <3C910CA2CB204A5A7261394D@CDYwxp1931.ad.med.buffalo.edu> For Researchers Only who have cryostats (1-2 hrs/wk ave usage): 1. Is your cryostat under service contract? 2. Have you had any problems with your cryostat that would justify having a service contract? Thank you! Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From kreaser <@t> vet.upenn.edu Tue Dec 1 13:19:56 2009 From: kreaser <@t> vet.upenn.edu (Karie Reaser) Date: Tue Dec 1 13:19:59 2009 Subject: [Histonet] Methylene Blue Message-ID: <2004411560.7539951259695196322.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> What is the best way to make Methylene Blue staining solution from powder? -- Karie L Reaser A.S. New Bolton Center University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Research Laboratory 382 W Street Road Kennett Square, PA. 19348 Phone:610-925-6278 Fax:610-925-8120 Email:kreaser@vet.upenn.edu From mward <@t> wfubmc.edu Tue Dec 1 13:28:13 2009 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Dec 1 13:28:29 2009 Subject: [Histonet] tech schedules Message-ID: <61135F0455D33347B5AAE209B903A30429D35278@EXCHVS2.medctr.ad.wfubmc.edu> Thanks to everyone who reponded to my question. I really appreciate everyone's comments and thoughts on the subject. Martha Ward Wake Forest University Baptist Medical Center From Michael.Owen <@t> fda.hhs.gov Tue Dec 1 13:25:51 2009 From: Michael.Owen <@t> fda.hhs.gov (Owen, Michael P) Date: Tue Dec 1 13:31:19 2009 Subject: [Histonet] Methylene Blue In-Reply-To: <2004411560.7539951259695196322.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> References: <2004411560.7539951259695196322.JavaMail.root@zm-mbx-levy.zimbra.upenn.edu> Message-ID: <6B1915839B67AD46B88E6BE8F27376BB19FBA0@FMD3VS031.fda.gov> Dear Karie, Are you preparing an acidic, neutral or alkaline methylene blue solution? I believe the simplest methylene blue solution I have encountered is 1% dye in distilled water. If you need to prepare an alkaline methylene blue stain, contact me. I have a list of several formulations. Sincerely, Michael From algranth <@t> email.arizona.edu Tue Dec 1 16:22:03 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Tue Dec 1 16:22:09 2009 Subject: [Histonet] Cryostat Poll In-Reply-To: <3C910CA2CB204A5A7261394D@CDYwxp1931.ad.med.buffalo.edu> References: <3C910CA2CB204A5A7261394D@CDYwxp1931.ad.med.buffalo.edu> Message-ID: yes and yes Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Dec 1, 2009, at 12:12 PM, Merced M Leiker wrote: > For Researchers Only who have cryostats (1-2 hrs/wk ave usage): > > 1. Is your cryostat under service contract? > > 2. Have you had any problems with your cryostat that would justify > having a service contract? > > Thank you! > > Merced M Leiker > Research Technician III > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Maria.Katleba <@t> stjoe.org Tue Dec 1 16:46:24 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Dec 1 16:46:47 2009 Subject: [Histonet] Cryostat Poll In-Reply-To: References: <3C910CA2CB204A5A7261394D@CDYwxp1931.ad.med.buffalo.edu> Message-ID: We have a LEICA CRYOSTAT... its EXCELLENT! Yes- we have a contract and NO never had issues... Maria Katleba HT(ASCP) MS Queen of the Valley Medical Center Napa CA 94558 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Tuesday, December 01, 2009 2:22 PM To: Merced M Leiker Cc: Histonet Subject: Re: [Histonet] Cryostat Poll yes and yes Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Dec 1, 2009, at 12:12 PM, Merced M Leiker wrote: > For Researchers Only who have cryostats (1-2 hrs/wk ave usage): > > 1. Is your cryostat under service contract? > > 2. Have you had any problems with your cryostat that would justify > having a service contract? > > Thank you! > > Merced M Leiker > Research Technician III > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > leiker@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From linresearch <@t> comcast.net Tue Dec 1 18:41:59 2009 From: linresearch <@t> comcast.net (linresearch@comcast.net) Date: Tue Dec 1 18:42:02 2009 Subject: [Histonet] (no subject) Message-ID: <1622249091.404131259714519163.JavaMail.root@sz0151a.westchester.pa.mail.comcast.net> Hello, Can anyone recommend PCNA antibody that works in FFPE rat tissue? Would you be willing to share your protocol? thanks, Lin From linresearch <@t> comcast.net Tue Dec 1 18:44:04 2009 From: linresearch <@t> comcast.net (linresearch@comcast.net) Date: Tue Dec 1 18:44:07 2009 Subject: [Histonet] PCNA Message-ID: <781386178.405021259714644025.JavaMail.root@sz0151a.westchester.pa.mail.comcast.net> Hello, Can anyone recommend PCNA antibody that works in FFPE rat tissue? Would you be willing to share your protocol? thanks, Lin From linresearch <@t> comcast.net Tue Dec 1 18:44:04 2009 From: linresearch <@t> comcast.net (linresearch@comcast.net) Date: Tue Dec 1 18:44:08 2009 Subject: [Histonet] PCNA Message-ID: <781386178.405021259714644025.JavaMail.root@sz0151a.westchester.pa.mail.comcast.net> Hello, Can anyone recommend PCNA antibody that works in FFPE rat tissue? Would you be willing to share your protocol? thanks, Lin From conniegrubaugh <@t> hotmail.com Tue Dec 1 20:02:38 2009 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Tue Dec 1 20:02:44 2009 Subject: [Histonet] tech schedules In-Reply-To: <963103.39298.qm@web65711.mail.ac4.yahoo.com> References: <61135F0455D33347B5AAE209B903A30429D3526C@EXCHVS2.medctr.ad.wfubmc.edu>, <963103.39298.qm@web65711.mail.ac4.yahoo.com> Message-ID: At our office we have 24 hour service and work six days a week. We all have fixed hours and it is much easier to stay on one shift then to be constantly changing everyones hours. More mistakes are made this way. Techs start coming in at 10:30 at night and some at midnight, 2:30 am 3, 5 6, 10am and then the next tech comes in at 1:30 pm. Some need to stay on the same shift to take care of their kids, some are attending classes at college and some of us are working part time in other offices, so it would be difficult to keep changing hours around. Connie G. > Date: Tue, 1 Dec 2009 10:19:54 -0800 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; mward@wfubmc.edu > Subject: Re: [Histonet] tech schedules > CC: > > I always rotated all the staff between 1, 2nd and 3rd shifts, and days of the week. > All ended working all shifts during the month. > They also rotated between tasks (embed, cut, special procedures). > Ren? J. > > --- On Tue, 12/1/09, Martha Ward wrote: > > > From: Martha Ward > Subject: [Histonet] tech schedules > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, December 1, 2009, 1:15 PM > > > I am posting this for my department manager. He is wondering how other > labs schedule their techs. In other words, do the techs work fixed > times (ie, come in always at 5 am) or do they rotate schedules (ie, come > in at 5 am for one month, then 6 am the next month, etc). I will pass > along your responses to him. Thanks in advance for any help you can > give. > > Martha Ward > Wake Forest University Baptist Medical Center > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Get gifts for them and cashback for you. Try Bing now. http://www.bing.com/shopping/search?q=xbox+games&scope=cashback&form=MSHYCB&publ=WLHMTAG&crea=TEXT_MSHYCB_Shopping_Giftsforthem_cashback_1x1 From richarddrahci <@t> gmail.com Wed Dec 2 04:58:07 2009 From: richarddrahci <@t> gmail.com (Richard J) Date: Wed Dec 2 04:58:13 2009 Subject: [Histonet] PCNA In-Reply-To: <781386178.405021259714644025.JavaMail.root@sz0151a.westchester.pa.mail.comcast.net> References: <781386178.405021259714644025.JavaMail.root@sz0151a.westchester.pa.mail.comcast.net> Message-ID: <88613e830912020258r53e1c401r93d997eb33e25f3d@mail.gmail.com> Looks like clone PC10 works well in rat, mouse, human, chick and zebrafish Protocols and images at http://www.immunoportal.com/modules.php?name=gallery2&g2_view=keyalbum.KeywordAlbum&g2_keyword=PCNA 2009/12/2 > > > Hello, > Can anyone recommend PCNA antibody that works in FFPE rat tissue? > Would you be willing to share your protocol? > thanks, > Lin > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tifei <@t> foxmail.com Wed Dec 2 05:14:25 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Wed Dec 2 05:45:09 2009 Subject: =?utf-8?B?UmU6IFJlOiBbSGlzdG9uZXRdIFBDTkE=?= References: <781386178.405021259714644025.JavaMail.root@sz0151a.westchester.pa.mail.comcast.net>, <88613e830912020258r53e1c401r93d997eb33e25f3d@mail.gmail.com> Message-ID: <200912021914244886478@foxmail.com> we use the cell signaling one. PCNA of mouse IgG 2009-12-02 TF ???? Richard J ????? 2009-12-02 19:04:06 ???? linresearch ??? histonet ??? Re: [Histonet] PCNA Looks like clone PC10 works well in rat, mouse, human, chick and zebrafish Protocols and images at http://www.immunoportal.com/modules.php?name=gallery2&g2_view=keyalbum.KeywordAlbum&g2_keyword=PCNA 2009/12/2 > > > Hello, > Can anyone recommend PCNA antibody that works in FFPE rat tissue? > Would you be willing to share your protocol? > thanks, > Lin > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdooley2008 <@t> yahoo.com Wed Dec 2 07:27:20 2009 From: jdooley2008 <@t> yahoo.com (James Dooley) Date: Wed Dec 2 07:27:24 2009 Subject: [Histonet] 3D reconstruction from histology slides Message-ID: <683934.37780.qm@web45901.mail.sp1.yahoo.com> Hello All, We want to do a 3D reconstruction from serial histology samples. On the we I have found Amira, BioVis 3D and Surf Driver software. If anyone could offer any input or? recommend one of these program it would be greatly appreciated. If there are any open source programs that could be used that would also be helpful. Thank you, James Dooley KULevuen, Belgium From PMonfils <@t> Lifespan.org Wed Dec 2 07:39:24 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Dec 2 07:39:29 2009 Subject: [Histonet] large coverslips In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835D0C@LSRIEXCH1.lsmaster.lifespan.org> Fisher Scientific carries 50x35 mm and 50x45 mm coverglass. From xipamanine <@t> gmail.com Wed Dec 2 08:35:54 2009 From: xipamanine <@t> gmail.com (Xipamanine Mkuze) Date: Wed Dec 2 08:36:00 2009 Subject: [Histonet] Autostainer service Message-ID: <3cfdeb590912020635ja3f443an67f1d1f3305d9c41@mail.gmail.com> Dako will no longer support Autostainers? Why? Ninni I need to pick your collective brains here about getting a service contract for my Dako Autostainers. I remember seeing a posting awhile back with the name of a company, I think from MD, that will service the Dako Autostainers now that Dako will no longer support them. If anyone has a suggestion for a company who will service these instruments or if anyone can remember the name of that company in MD, please contact me. Happy Monday everyone, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 From Loralee_Mcmahon <@t> URMC.Rochester.edu Wed Dec 2 08:39:22 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Wed Dec 2 08:40:14 2009 Subject: [Histonet] Autostainer service In-Reply-To: <3cfdeb590912020635ja3f443an67f1d1f3305d9c41@mail.gmail.com> References: <3cfdeb590912020635ja3f443an67f1d1f3305d9c41@mail.gmail.com> Message-ID: I think that they are not supporting the older model of autostainer. They are still selling a new version of the autostainer. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Xipamanine Mkuze [xipamanine@gmail.com] Sent: Wednesday, December 02, 2009 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Autostainer service Dako will no longer support Autostainers? Why? Ninni I need to pick your collective brains here about getting a service contract for my Dako Autostainers. I remember seeing a posting awhile back with the name of a company, I think from MD, that will service the Dako Autostainers now that Dako will no longer support them. If anyone has a suggestion for a company who will service these instruments or if anyone can remember the name of that company in MD, please contact me. Happy Monday everyone, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Wed Dec 2 08:41:58 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Dec 2 08:42:03 2009 Subject: [Histonet] Autostainer service ...Dako would you respond? In-Reply-To: References: <3cfdeb590912020635ja3f443an67f1d1f3305d9c41@mail.gmail.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D835B0@EMAIL.archildrens.org> Dako would you respond to this issue? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Wednesday, December 02, 2009 8:39 AM To: Xipamanine Mkuze; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Autostainer service I think that they are not supporting the older model of autostainer. They are still selling a new version of the autostainer. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Xipamanine Mkuze [xipamanine@gmail.com] Sent: Wednesday, December 02, 2009 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Autostainer service Dako will no longer support Autostainers? Why? Ninni I need to pick your collective brains here about getting a service contract for my Dako Autostainers. I remember seeing a posting awhile back with the name of a company, I think from MD, that will service the Dako Autostainers now that Dako will no longer support them. If anyone has a suggestion for a company who will service these instruments or if anyone can remember the name of that company in MD, please contact me. Happy Monday everyone, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From WBENTON <@t> umm.edu Wed Dec 2 08:58:27 2009 From: WBENTON <@t> umm.edu (Walter Benton) Date: Wed Dec 2 08:58:48 2009 Subject: [Histonet] Tech schedules In-Reply-To: <787361B4.499@GWIA2.umm.edu> References: <787361B4.499@GWIA2.umm.edu> Message-ID: <4B163A42.D886.00F4.3@umm.edu> We have fixed tech schedules, but we rotate task within the lab weekly to keep everyone fresh and competent. Our shifts include 4am (3) 6am (2) 7am (2) 8am (1) Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From dholmes <@t> anatomy.umsmed.edu Wed Dec 2 09:27:09 2009 From: dholmes <@t> anatomy.umsmed.edu (Dianne Holmes) Date: Wed Dec 2 09:27:33 2009 Subject: [Histonet] large coverglass Message-ID: <4B1632ED0200008200046619@GWIA1.umsmed.edu> Brain Research Laboratories has anything up to 4"x 5". We use them for monkey slides. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From aevans <@t> wellspan.org Wed Dec 2 09:38:08 2009 From: aevans <@t> wellspan.org (Evans, Andria B) Date: Wed Dec 2 09:43:30 2009 Subject: [Histonet] Cerner Millenium Message-ID: Does anyone out there in Histoland use Cerner Millenium in there lab? I have some questions if you could contact me that would be great. Thanks! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From trathborne <@t> somerset-healthcare.com Wed Dec 2 09:43:30 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Dec 2 09:43:36 2009 Subject: [Histonet] Tech schedules In-Reply-To: <4B163A42.D886.00F4.3@umm.edu> Message-ID: Walter, I'm just curious - how many pathologists do you have? How many tissue processors? and what is your average TAT? Thanks, Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Walter Benton Sent: Wednesday, December 02, 2009 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tech schedules We have fixed tech schedules, but we rotate task within the lab weekly to keep everyone fresh and competent. Our shifts include 4am (3) 6am (2) 7am (2) 8am (1) Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From portera <@t> msu.edu Wed Dec 2 09:45:35 2009 From: portera <@t> msu.edu (Amy Porter) Date: Wed Dec 2 09:46:05 2009 Subject: [Histonet] Autostainer service ...Dako would you respond? References: <3cfdeb590912020635ja3f443an67f1d1f3305d9c41@mail.gmail.com> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D835B0@EMAIL.archildrens.org> Message-ID: I just renewed my service contract for my autostainer which is 6 years old - no problem. Just an FYI. Amy Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Horn, Hazel V" To: "McMahon, Loralee A" ; "Xipamanine Mkuze" ; Sent: Wednesday, December 02, 2009 9:41 AM Subject: RE: [Histonet] Autostainer service ...Dako would you respond? Dako would you respond to this issue? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Wednesday, December 02, 2009 8:39 AM To: Xipamanine Mkuze; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Autostainer service I think that they are not supporting the older model of autostainer. They are still selling a new version of the autostainer. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Xipamanine Mkuze [xipamanine@gmail.com] Sent: Wednesday, December 02, 2009 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Autostainer service Dako will no longer support Autostainers? Why? Ninni I need to pick your collective brains here about getting a service contract for my Dako Autostainers. I remember seeing a posting awhile back with the name of a company, I think from MD, that will service the Dako Autostainers now that Dako will no longer support them. If anyone has a suggestion for a company who will service these instruments or if anyone can remember the name of that company in MD, please contact me. Happy Monday everyone, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ken_Marissael <@t> vwr.com Wed Dec 2 09:56:33 2009 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Wed Dec 2 09:56:50 2009 Subject: [Histonet] large coverslips In-Reply-To: <200912021426.nB2EPuQw022354@appliance01.vwr.com> Message-ID: The largest production coverslip that I have heard of is a 25mm square Ken Marissael VWR Clinical Account Representative ken_marissael@vwr.com From azdudley <@t> hotmail.com Wed Dec 2 10:00:53 2009 From: azdudley <@t> hotmail.com (anita dudley) Date: Wed Dec 2 10:00:57 2009 Subject: [Histonet] her 2 validation Message-ID: good morning!! just wanted to make sure about the number of slides that are required for validation for her 2. is it 25? thanks so much, anita dudley providence hospital mobile alabama _________________________________________________________________ Windows Live Hotmail gives you a free,exclusive gift. http://www.microsoft.com/windows/windowslive/hotmail_bl1/hotmail_bl1.aspx?ocid=PID23879::T:WLMTAGL:ON:WL:en-ww:WM_IMHM_7:092009 From tifei <@t> foxmail.com Wed Dec 2 10:01:02 2009 From: tifei <@t> foxmail.com (TF) Date: Wed Dec 2 10:01:08 2009 Subject: [Histonet] Trophic factors staining on brain sections References: <781386178.405021259714644025.JavaMail.root@sz0151a.westchester.pa.mail.comcast.net>, <88613e830912020258r53e1c401r93d997eb33e25f3d@mail.gmail.com> Message-ID: <200912030001012549893@foxmail.com> Hi all to stain out the trophic factors on brain sections, such as BDNFm, IGF1, NGF, CNTF, GDNF will these molecules "leak" out during staining? so I should not use triton and try to shorten the time of staining? Or I need a long time incubation to stain out the "low" concentration small molecules? thanks! 2009-12-02 TF From shive003 <@t> umn.edu Wed Dec 2 10:07:41 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Dec 2 10:07:45 2009 Subject: [Histonet] Autostainer service References: <3cfdeb590912020635ja3f443an67f1d1f3305d9c41@mail.gmail.com> Message-ID: I was told that they would not extend annual maintenance agreements to my models older than 5 years, but they will come to service older models on a repair or emergency call/fee-for-service basis. Jan Shivers ----- Original Message ----- From: "McMahon, Loralee A" To: "Xipamanine Mkuze" ; Sent: Wednesday, December 02, 2009 8:39 AM Subject: RE: [Histonet] Autostainer service I think that they are not supporting the older model of autostainer. They are still selling a new version of the autostainer. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Xipamanine Mkuze [xipamanine@gmail.com] Sent: Wednesday, December 02, 2009 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Autostainer service Dako will no longer support Autostainers? Why? Ninni I need to pick your collective brains here about getting a service contract for my Dako Autostainers. I remember seeing a posting awhile back with the name of a company, I think from MD, that will service the Dako Autostainers now that Dako will no longer support them. If anyone has a suggestion for a company who will service these instruments or if anyone can remember the name of that company in MD, please contact me. Happy Monday everyone, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MMargiotta <@t> bmhmc.org Wed Dec 2 10:15:02 2009 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Wed Dec 2 10:15:09 2009 Subject: [Histonet] gross only specimens Message-ID: Hi All, There is a new standard from JCAHO addressing which tissue specimens require only a macroscopic examination. Does anyone have a list of specimens that fit this standard. We are in the process of writing up a policy for this and would appreciate your help. Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From Joanne.Taylor <@t> gov.bc.ca Wed Dec 2 10:27:59 2009 From: Joanne.Taylor <@t> gov.bc.ca (Taylor, Joanne AL:EX) Date: Wed Dec 2 10:28:08 2009 Subject: [Histonet] Mycoplasma special stain Message-ID: <9C810CFC93F92D49B96B29033CFF813F0337EF5A@span.idir.bcgov> I'm looking for a special stain procedure for mycoplasma. Would the modified (low pH) Warthin-Starry method work? Thanks, JoAnne From lost.dragonfly <@t> yahoo.com Wed Dec 2 10:49:33 2009 From: lost.dragonfly <@t> yahoo.com (Una McGiven) Date: Wed Dec 2 10:49:37 2009 Subject: [Histonet] CAP question Message-ID: <268877.54205.qm@web110303.mail.gq1.yahoo.com> Hi everyone, ? On the most recent CAP checklist there is a requirement for 2 patient identifiers.? For those labs who are CAP and receive all or a large portion of their cases from outside facilities (=not from your facility), what are you using as the identifiers on your specimen containers?? Name and birthdate?? SS#?? Med record?? Something else? ? Thank you in advance! From Michael.Owen <@t> fda.hhs.gov Wed Dec 2 10:46:02 2009 From: Michael.Owen <@t> fda.hhs.gov (Owen, Michael P) Date: Wed Dec 2 10:52:28 2009 Subject: [Histonet] Histology Technician Position - Seattle Children's Hospital Message-ID: <6B1915839B67AD46B88E6BE8F27376BB19FBA5@FMD3VS031.fda.gov> WashingtonLifeScience.com - Career Opportunities http://www.washingtonlifescience.com/career/career-opp.htm http://www.washingtonlifescience.com/showjob?jobid=5763 Seattle Children's Hospital Histology Technician JOB FUNCTION: Histology JOB DESCRIPTION: We invite you to bring your career to an environment where talent is rewarded and new ideas are encouraged. At Seattle Children's, the Pacific Northwest's premier pediatric care center, we offer more than just state-of-the-art facilities and open career growth potential. You will also find a true commitment to meeting the needs of children and their families. We value diversity and it is expressed in all aspects, from the patients and families we serve to our organizational culture and our employees. If you would like to do some of your best work-your life's work-Children's has excellent opportunities waiting for you. Department: Anatomical Pathology Salary: DOE Hours: Full time, days. Location: Hospital Main Campus, Seattle, WA Provide histology testing in the anatomic pathology laboratory, supporting the Department of Pathology and Laboratories mission and business strategies, which support the diagnosis and treatment of pediatric patients in the community and region. Required: Associate degree in histotechnology from an accredited college or approved hospital-based histotechnology program, or equivalent combination of education and experience. HT or HTL (ASCP) or equivalent certification. Preferred: Five (5) years of experience in a diverse clinical laboratory setting. JOB REQUIREMENTS: At Seattle Children's, we believe in accountability, respect and teamwork - not only with patients and their families, but also with each other. If you share these principles, we encourage you to join us. We offer excellent pay and benefits, retirement plans, opportunities for career advancement, paid training days, and so much more. For immediate consideration, please apply online. To Apply for this position, please CLICK HERE. http://childrens.contacthr.com/14619367 Seattle Children's Hospital http://childrens.contacthr.com/14619367 Reference Job Code: KQ102421-105593WBI Attn: Kate Quaak Seattle Children's Hospital is an Equal Opportunity Employer Submitted: 11/24/2009 Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From JWeems <@t> sjha.org Wed Dec 2 10:59:33 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Dec 2 10:59:35 2009 Subject: [Histonet] CAP question In-Reply-To: <268877.54205.qm@web110303.mail.gq1.yahoo.com> References: <268877.54205.qm@web110303.mail.gq1.yahoo.com> Message-ID: Case number and name.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Una McGiven Sent: Wednesday, December 02, 2009 11:50 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question Hi everyone, ? On the most recent CAP checklist there is a requirement for 2 patient identifiers.? For those labs who are CAP and receive all or a large portion of their cases from outside facilities (=not from your facility), what are you using as the identifiers on your specimen containers?? Name and birthdate?? SS#?? Med record?? Something else? ? Thank you in advance! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From landers <@t> jhmi.edu Wed Dec 2 11:04:14 2009 From: landers <@t> jhmi.edu (Lois Anderson) Date: Wed Dec 2 11:04:08 2009 Subject: [Histonet] CAP question In-Reply-To: References: <268877.54205.qm@web110303.mail.gq1.yahoo.com> Message-ID: <5D8D0C04664923468020963BA314078A32A4019A@JHEMTEXVS3.win.ad.jhu.edu> Name and case number or med records number -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 02, 2009 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Case number and name.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Una McGiven Sent: Wednesday, December 02, 2009 11:50 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question Hi everyone, ? On the most recent CAP checklist there is a requirement for 2 patient identifiers.? For those labs who are CAP and receive all or a large portion of their cases from outside facilities (=not from your facility), what are you using as the identifiers on your specimen containers?? Name and birthdate?? SS#?? Med record?? Something else? ? Thank you in advance! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Anna.Inman <@t> stmarygj.org Wed Dec 2 11:15:55 2009 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Wed Dec 2 11:16:10 2009 Subject: [Histonet] CAP question In-Reply-To: <5D8D0C04664923468020963BA314078A32A4019A@JHEMTEXVS3.win.ad.jhu.edu> References: <268877.54205.qm@web110303.mail.gq1.yahoo.com> <5D8D0C04664923468020963BA314078A32A4019A@JHEMTEXVS3.win.ad.jhu.edu> Message-ID: <2925AE271EAAD440AF48FCCEB8002D0908B813BF@smgmail01.smgj.sclhs.net> Keep in mind: The requirement states 2 patient identifiers at the time of collection - so the identifiers need to come from the outside entities. My conversation with CAP - they are going for patient name, DOB, SS# or medical record number of some sort that ties the hospital to the clinician office. If the case number or med record number does not get joined until it reaches your facility - it does not meet the CAP requirement. We are asking for patient name and DOB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lois Anderson Sent: Wednesday, December 02, 2009 10:04 AM To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Name and case number or med records number -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 02, 2009 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Case number and name.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Una McGiven Sent: Wednesday, December 02, 2009 11:50 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question Hi everyone, On the most recent CAP checklist there is a requirement for 2 patient identifiers. For those labs who are CAP and receive all or a large portion of their cases from outside facilities (=not from your facility), what are you using as the identifiers on your specimen containers? Name and birthdate? SS#? Med record? Something else? Thank you in advance! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From becky.garrison <@t> jax.ufl.edu Wed Dec 2 11:28:14 2009 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Wed Dec 2 11:28:18 2009 Subject: [Histonet] CAP question In-Reply-To: <268877.54205.qm@web110303.mail.gq1.yahoo.com> References: <268877.54205.qm@web110303.mail.gq1.yahoo.com> Message-ID: JCAHO standard is patient name and date of birth for the 2 unique identifiers. Becky Garrison Pathology Supervisor Shands Jacksonville 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Una McGiven Sent: Wednesday, December 02, 2009 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question Hi everyone, ? On the most recent CAP checklist there is a requirement for 2 patient identifiers.? For those labs who are CAP and receive all or a large portion of their cases from outside facilities (=not from your facility), what are you using as the identifiers on your specimen containers?? Name and birthdate?? SS#?? Med record?? Something else? ? Thank you in advance! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Donadio <@t> bhcpns.org Wed Dec 2 11:34:01 2009 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Wed Dec 2 11:34:25 2009 Subject: [Histonet] CAP question In-Reply-To: Message-ID: That's what we use as well. Patient name and DOB Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From JWeems <@t> sjha.org Wed Dec 2 11:40:55 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Dec 2 11:40:59 2009 Subject: [Histonet] CAP question In-Reply-To: <2925AE271EAAD440AF48FCCEB8002D0908B813BF@smgmail01.smgj.sclhs.net> References: <268877.54205.qm@web110303.mail.gq1.yahoo.com> <5D8D0C04664923468020963BA314078A32A4019A@JHEMTEXVS3.win.ad.jhu.edu> <2925AE271EAAD440AF48FCCEB8002D0908B813BF@smgmail01.smgj.sclhs.net> Message-ID: Don't we still need to follow through with two identifiers tho? ________________________________ From: Inman, Anna [mailto:Anna.Inman@stmarygj.org] Sent: Wednesday, December 02, 2009 12:16 To: Lois Anderson; Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Keep in mind: The requirement states 2 patient identifiers at the time of collection - so the identifiers need to come from the outside entities. My conversation with CAP - they are going for patient name, DOB, SS# or medical record number of some sort that ties the hospital to the clinician office. If the case number or med record number does not get joined until it reaches your facility - it does not meet the CAP requirement. We are asking for patient name and DOB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lois Anderson Sent: Wednesday, December 02, 2009 10:04 AM To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Name and case number or med records number -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 02, 2009 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Case number and name.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Una McGiven Sent: Wednesday, December 02, 2009 11:50 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question Hi everyone, On the most recent CAP checklist there is a requirement for 2 patient identifiers. For those labs who are CAP and receive all or a large portion of their cases from outside facilities (=not from your facility), what are you using as the identifiers on your specimen containers? Name and birthdate? SS#? Med record? Something else? Thank you in advance! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From histosprv06 <@t> aol.com Wed Dec 2 11:59:40 2009 From: histosprv06 <@t> aol.com (histosprv06@aol.com) Date: Wed Dec 2 12:00:01 2009 Subject: [Histonet] FL Supervisor License Message-ID: <8CC41905ADA46DF-44FC-8136@webmail-d088.sysops.aol.com> This may have been covered already, please accept my apologies for a repeat question. I was curious if anyone knows if you can upgrade a technologist (FL state license only, grandfathered in so NO ASCP) license WITHOUT a 4 year degree? The work experience is 10+ years but this person does NOT have a 2 or 4 year degree of any kind. I know the laws constantly change, was just curious about the current ones, I am not familiar with ASCP guidelines for FL. Thank you in advance. Marie From HornHV <@t> archildrens.org Wed Dec 2 12:05:42 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Dec 2 12:05:46 2009 Subject: [Histonet] gross only specimens In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D835B7@EMAIL.archildrens.org> I believe your surgical affairs committee can compose this list. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, Michele Sent: Wednesday, December 02, 2009 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] gross only specimens Hi All, There is a new standard from JCAHO addressing which tissue specimens require only a macroscopic examination. Does anyone have a list of specimens that fit this standard. We are in the process of writing up a policy for this and would appreciate your help. Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Ken_Marissael <@t> vwr.com Wed Dec 2 12:36:08 2009 From: Ken_Marissael <@t> vwr.com (Ken_Marissael@vwr.com) Date: Wed Dec 2 12:36:13 2009 Subject: [Histonet] large coverglass In-Reply-To: <200912021737.nB2HbM5v023053@appliance01.vwr.com> Message-ID: I stand corrected! Mike from Erie (Thermofisher) let me know. You may want to give him a call...see e-mail below. "I saw your Cover Glass e-mail on the Histonet, and wanted to let you know that we can do any custom sizes of cover glass. We can make rectangles, squares & circles to any specification that your customers may need. We can do sizes larger than 25mm squares." Michael Vadeboncoeur Product Manager Slides/Specialty Glass/Handcare michael.vadeboncoeur@thermofisher.com Ken Marissael VWR Clinical Account Representative ken_marissael@vwr.com From aevans <@t> wellspan.org Wed Dec 2 12:40:46 2009 From: aevans <@t> wellspan.org (Evans, Andria B) Date: Wed Dec 2 12:40:51 2009 Subject: [Histonet] Re: Tech Sechedules Message-ID: In our lab we have set hours for each histotech and the duties rotate weekly. The hours we have are.... 4:30, 5:00, 5:30, 6:00, 6:30, 7:00, 7:30, 8:15, 8:30(x2) Each time slot has 1 histotech that comes in at the times above except the 8:30 slot, that has 2 people. If you have any other questions please feel free to ask. Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From DKBoyd <@t> chs.net Wed Dec 2 12:43:02 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Dec 2 12:42:30 2009 Subject: [Histonet] gross only specimens In-Reply-To: Message-ID: We have a policy which includes a list of tissues that are not required to be sent to the lab as well as a list of gross only specimens. This policy is presented at the Medical Staff meeting and is approved in that committee. Changes are made through the same committee. The Laboratory Medical Director and the Chief of Surgical Services both sign off on the policy. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net "Margiotta, Michele" Sent by: histonet-bounces@lists.utsouthwestern.edu 12/02/2009 11:16 AM To cc Subject [Histonet] gross only specimens Hi All, There is a new standard from JCAHO addressing which tissue specimens require only a macroscopic examination. Does anyone have a list of specimens that fit this standard. We are in the process of writing up a policy for this and would appreciate your help. Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From rjbuesa <@t> yahoo.com Wed Dec 2 13:53:49 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 2 13:53:54 2009 Subject: [Histonet] FL Supervisor License In-Reply-To: <8CC41905ADA46DF-44FC-8136@webmail-d088.sysops.aol.com> Message-ID: <756637.96938.qm@web65710.mail.ac4.yahoo.com> As far as I know, a 4 years degree is necessary to be licensed as Supervisor in Florida. Ren? J. --- On Wed, 12/2/09, histosprv06@aol.com wrote: From: histosprv06@aol.com Subject: [Histonet] FL Supervisor License To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 2, 2009, 12:59 PM This may have been covered already, please accept my apologies for a repeat question. I was curious if anyone knows if you can upgrade a technologist (FL state license only, grandfathered in so NO ASCP) license WITHOUT a 4 year degree? The work experience is 10+ years but this person does NOT have a 2 or 4 year degree of any kind. I know the laws constantly change, was just curious about the current ones, I am not familiar with ASCP guidelines for FL. Thank you in advance. Marie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Anna.Inman <@t> stmarygj.org Wed Dec 2 14:05:42 2009 From: Anna.Inman <@t> stmarygj.org (Inman, Anna) Date: Wed Dec 2 14:06:08 2009 Subject: [Histonet] CAP question In-Reply-To: References: <268877.54205.qm@web110303.mail.gq1.yahoo.com><5D8D0C04664923468020963BA314078A32A4019A@JHEMTEXVS3.win.ad.jhu.edu><2925AE271EAAD440AF48FCCEB8002D0908B813BF@smgmail01.smgj.sclhs.net> Message-ID: <2925AE271EAAD440AF48FCCEB8002D0908B813C1@smgmail01.smgj.sclhs.net> Yes, patient name, DOB or SS# and the case number and such is "extra" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 02, 2009 10:41 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Don't we still need to follow through with two identifiers tho? ________________________________ From: Inman, Anna [mailto:Anna.Inman@stmarygj.org] Sent: Wednesday, December 02, 2009 12:16 To: Lois Anderson; Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Keep in mind: The requirement states 2 patient identifiers at the time of collection - so the identifiers need to come from the outside entities. My conversation with CAP - they are going for patient name, DOB, SS# or medical record number of some sort that ties the hospital to the clinician office. If the case number or med record number does not get joined until it reaches your facility - it does not meet the CAP requirement. We are asking for patient name and DOB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lois Anderson Sent: Wednesday, December 02, 2009 10:04 AM To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Name and case number or med records number -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 02, 2009 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Case number and name.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Una McGiven Sent: Wednesday, December 02, 2009 11:50 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question Hi everyone, On the most recent CAP checklist there is a requirement for 2 patient identifiers. For those labs who are CAP and receive all or a large portion of their cases from outside facilities (=not from your facility), what are you using as the identifiers on your specimen containers? Name and birthdate? SS#? Med record? Something else? Thank you in advance! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From liz <@t> premierlab.com Wed Dec 2 14:13:06 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Dec 2 14:13:12 2009 Subject: [Histonet] procedure for osmium and potassium chromate? Message-ID: I'm trying to find a procedure for staining fat in tissues with osmium and I think potassium dichromate, does anyone out there have this protocol available. I have found it before on the web but for some reason I can't find it this time. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 From mcauliff <@t> umdnj.edu Wed Dec 2 14:40:54 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Dec 2 14:38:58 2009 Subject: [Histonet] procedure for osmium and potassium chromate? In-Reply-To: References: Message-ID: <4B16D0D6.8010205@umdnj.edu> Dalton's Chrome-osmium fix was popular for awhile in the 1950's. You will need to look in an older methods book. The combo of dichromate and osmium will not make your safety officer happy. There are many ways to stain lipids with osmium, alone or mixed with something else. Is this for EM or LM? Geoff Liz Chlipala wrote: > I'm trying to find a procedure for staining fat in tissues with osmium > and I think potassium dichromate, does anyone out there have this > protocol available. I have found it before on the web but for some > reason I can't find it this time. > > > > Liz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > > Manager > > Premier Laboratory, LLC > > PO Box 18592 > > Boulder, Colorado 80308 > > office (303) 682-3949 > > fax (303) 682-9060 > > www.premierlab.com > > > > > > Ship to Address: > > 1567 Skyway Drive, Unit E > > Longmont, Colorado 80504 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From liz <@t> premierlab.com Wed Dec 2 15:03:24 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Dec 2 15:03:30 2009 Subject: [Histonet] procedure for osmium and potassium chromate? In-Reply-To: <4B16D0D6.8010205@umdnj.edu> Message-ID: Light Micro. I have done this before and it turned out great on some mouse muscle, but I misplaced my procedure. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Sent: Wednesday, December 02, 2009 1:41 PM To: Liz Chlipala Cc: histonet@pathology.swmed.edu Subject: Re: [Histonet] procedure for osmium and potassium chromate? Dalton's Chrome-osmium fix was popular for awhile in the 1950's. You will need to look in an older methods book. The combo of dichromate and osmium will not make your safety officer happy. There are many ways to stain lipids with osmium, alone or mixed with something else. Is this for EM or LM? Geoff Liz Chlipala wrote: > I'm trying to find a procedure for staining fat in tissues with osmium > and I think potassium dichromate, does anyone out there have this > protocol available. I have found it before on the web but for some > reason I can't find it this time. > > > > Liz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > > Manager > > Premier Laboratory, LLC > > PO Box 18592 > > Boulder, Colorado 80308 > > office (303) 682-3949 > > fax (303) 682-9060 > > www.premierlab.com > > > > > > Ship to Address: > > 1567 Skyway Drive, Unit E > > Longmont, Colorado 80504 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From cpyse <@t> x-celllab.com Wed Dec 2 15:05:59 2009 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Wed Dec 2 15:06:51 2009 Subject: [Histonet] procedure for osmium and potassium chromate? In-Reply-To: References: Message-ID: <000001ca7393$3fe46580$bfad3080$@com> OSMIUM TETROXIDE FOR FAT (Paraffin SECTIONS) Solutions 2.5% Potassium Dichromate Solution Stock 1% Osmium Tetroxide Solution Stock Potassium Dichromate-Osmium Tetroxide Solution Working Potassium Dichromate Stock 50 ml Osmium Tetroxide stock 50 ml Staining Procedure 1. Place formalin fixed specimen, no thicker than 4mm, in working potassium dichromate-osmium tetroxide solution for up to 16 hours. 2. Wash specimen in running water for 2 hours. 3. Process tissue, embed in paraffin and cut at 4 microns. 4. Stain in routine H&E staining setup, than mount. Results: Fat - black Background - same as H&E stain. Hope this helps. If you have any questions please let me know. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, December 02, 2009 3:13 PM To: histonet@pathology.swmed.edu Subject: [Histonet] procedure for osmium and potassium chromate? I'm trying to find a procedure for staining fat in tissues with osmium and I think potassium dichromate, does anyone out there have this protocol available. I have found it before on the web but for some reason I can't find it this time. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Dec 2 15:15:03 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 2 15:15:10 2009 Subject: [Histonet] procedure for osmium and potassium chromate? In-Reply-To: Message-ID: <405109.33036.qm@web65705.mail.ac4.yahoo.com> I really would suggest you to find an alternative procedure. Osmium tetroxyde is EXTREMELY dangerous and has to be used under extreme precautions. I don't think the results are worth the effort given the great dangers it poses. I for one would not share the procedure with you. Ren? J. --- On Wed, 12/2/09, Liz Chlipala wrote: From: Liz Chlipala Subject: [Histonet] procedure for osmium and potassium chromate? To: histonet@pathology.swmed.edu Date: Wednesday, December 2, 2009, 3:13 PM I'm trying to find a procedure for staining fat in tissues with osmium and I think potassium dichromate, does anyone out there have this protocol available.? I have found it before on the web but for some reason I can't? find it this time. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Dec 2 15:21:20 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 2 15:21:25 2009 Subject: [Histonet] CAP question In-Reply-To: <2925AE271EAAD440AF48FCCEB8002D0908B813C1@smgmail01.smgj.sclhs.net> Message-ID: <319971.90412.qm@web65701.mail.ac4.yahoo.com> I would strongly suggest not using the SS# as a second identifier. That is a piece of information the patient gave to the hospital but I am sure the patient would not like to see written in a cassette/slide for all to see (and copy). Ren? J. --- On Wed, 12/2/09, Inman, Anna wrote: From: Inman, Anna Subject: RE: [Histonet] CAP question To: "Weems, Joyce" , histonet@lists.utsouthwestern.edu Date: Wednesday, December 2, 2009, 3:05 PM Yes, patient name, DOB or SS#? and the case number and such is "extra" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 02, 2009 10:41 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Don't we still need to follow through with two identifiers tho? ________________________________ From: Inman, Anna [mailto:Anna.Inman@stmarygj.org] Sent: Wednesday, December 02, 2009 12:16 To: Lois Anderson; Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Keep in mind: The requirement states 2 patient identifiers at the time of collection - so the identifiers need to come from the outside entities. My conversation with CAP - they are going for patient name, DOB, SS# or medical record number of some sort that ties the hospital to the clinician office. If the case number or med record number does not get joined until it reaches your facility - it does not meet the CAP requirement. We are asking for patient name and DOB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lois Anderson Sent: Wednesday, December 02, 2009 10:04 AM To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Name and case number or med records number -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 02, 2009 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Case number and name.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Una McGiven Sent: Wednesday, December 02, 2009 11:50 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question Hi everyone, On the most recent CAP checklist there is a requirement for 2 patient identifiers.? For those labs who are CAP and receive all or a large portion of their cases from outside facilities (=not from your facility), what are you using as the identifiers on your specimen containers?? Name and birthdate?? SS#?? Med record?? Something else? Thank you in advance! ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE:? This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE:? This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Wed Dec 2 15:25:10 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Dec 2 15:27:08 2009 Subject: [Histonet] RE: Mycoplasma special stain In-Reply-To: <20091202180034.4973A486F8@barracuda.sdstate.edu> References: <20091202180034.4973A486F8@barracuda.sdstate.edu> Message-ID: Hi JoAnne, Our pathologist requested we try a Faulkner stain for Mycoplasma. I found two methods. The Faulkner/Lillie and the Faulkner/Kerr variations of the Warthin Starry. One of these is a lower acid. The pathologist is looking up the reference and will call the people who wrote a paper on this. I can let you know what I find out. We would like to find a quicker method than PCR and we've tried several antibodies for IHC but they seem to cross react with other bacteria. Margaret Perry Margaret.Perry@sdstate.edu From LSebree <@t> uwhealth.org Wed Dec 2 15:32:18 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Dec 2 15:32:25 2009 Subject: [Histonet] CAP question In-Reply-To: <319971.90412.qm@web65701.mail.ac4.yahoo.com> Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF6AE@UWHC-MAIL01.uwhis.hosp.wisc.edu> Leads me to wonder how the VA gets away with using SS#s as identifiers! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, December 02, 2009 3:21 PM To: JoyceWeems; histonet@lists.utsouthwestern.edu; AnnaInman Subject: RE: [Histonet] CAP question I would strongly suggest not using the SS# as a second identifier. That is a piece of information the patient gave to the hospital but I am sure the patient would not like to see written in a cassette/slide for all to see (and copy). Ren? J. --- On Wed, 12/2/09, Inman, Anna wrote: From: Inman, Anna Subject: RE: [Histonet] CAP question To: "Weems, Joyce" , histonet@lists.utsouthwestern.edu Date: Wednesday, December 2, 2009, 3:05 PM Yes, patient name, DOB or SS#? and the case number and such is "extra" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 02, 2009 10:41 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Don't we still need to follow through with two identifiers tho? ________________________________ From: Inman, Anna [mailto:Anna.Inman@stmarygj.org] Sent: Wednesday, December 02, 2009 12:16 To: Lois Anderson; Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Keep in mind: The requirement states 2 patient identifiers at the time of collection - so the identifiers need to come from the outside entities. My conversation with CAP - they are going for patient name, DOB, SS# or medical record number of some sort that ties the hospital to the clinician office. If the case number or med record number does not get joined until it reaches your facility - it does not meet the CAP requirement. We are asking for patient name and DOB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lois Anderson Sent: Wednesday, December 02, 2009 10:04 AM To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Name and case number or med records number -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 02, 2009 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Case number and name.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Una McGiven Sent: Wednesday, December 02, 2009 11:50 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question Hi everyone, On the most recent CAP checklist there is a requirement for 2 patient identifiers.? For those labs who are CAP and receive all or a large portion of their cases from outside facilities (=not from your facility), what are you using as the identifiers on your specimen containers?? Name and birthdate?? SS#?? Med record?? Something else? Thank you in advance! ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE:? This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE:? This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Dec 2 15:39:06 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 2 15:39:11 2009 Subject: [Histonet] CAP question In-Reply-To: <8C023B4AB999614BA4791BAEB26E27381BF6AE@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <144559.19221.qm@web65702.mail.ac4.yahoo.com> Because those whose SS# are for everybody to see do not know! Ren? J. --- On Wed, 12/2/09, Sebree Linda A wrote: From: Sebree Linda A Subject: RE: [Histonet] CAP question To: "Rene J Buesa" , "JoyceWeems" , histonet@lists.utsouthwestern.edu, "AnnaInman" Date: Wednesday, December 2, 2009, 4:32 PM Leads me to wonder how the VA gets away with using SS#s as identifiers! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, December 02, 2009 3:21 PM To: JoyceWeems; histonet@lists.utsouthwestern.edu; AnnaInman Subject: RE: [Histonet] CAP question I would strongly suggest not using the SS# as a second identifier. That is a piece of information the patient gave to the hospital but I am sure the patient would not like to see written in a cassette/slide for all to see (and copy). Ren? J. --- On Wed, 12/2/09, Inman, Anna wrote: From: Inman, Anna Subject: RE: [Histonet] CAP question To: "Weems, Joyce" , histonet@lists.utsouthwestern.edu Date: Wednesday, December 2, 2009, 3:05 PM Yes, patient name, DOB or SS#? and the case number and such is "extra" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 02, 2009 10:41 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Don't we still need to follow through with two identifiers tho? ________________________________ From: Inman, Anna [mailto:Anna.Inman@stmarygj.org] Sent: Wednesday, December 02, 2009 12:16 To: Lois Anderson; Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Keep in mind: The requirement states 2 patient identifiers at the time of collection - so the identifiers need to come from the outside entities. My conversation with CAP - they are going for patient name, DOB, SS# or medical record number of some sort that ties the hospital to the clinician office. If the case number or med record number does not get joined until it reaches your facility - it does not meet the CAP requirement. We are asking for patient name and DOB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lois Anderson Sent: Wednesday, December 02, 2009 10:04 AM To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Name and case number or med records number -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, December 02, 2009 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP question Case number and name.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Una McGiven Sent: Wednesday, December 02, 2009 11:50 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question Hi everyone, On the most recent CAP checklist there is a requirement for 2 patient identifiers.? For those labs who are CAP and receive all or a large portion of their cases from outside facilities (=not from your facility), what are you using as the identifiers on your specimen containers?? Name and birthdate?? SS#?? Med record?? Something else? Thank you in advance! ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE:? This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE:? This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed Dec 2 15:42:24 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Dec 2 15:42:47 2009 Subject: [Histonet] Histotechnician/Histotechnologist position, Veterinary Molecular Biology, Montana State University, Bozeman MT (VERY LONG message) Message-ID: <000001ca7398$575baed0$06130c70$@callis@bresnan.net> Dear Histonetters Our laboratory has advertised for a registered HT or HTL (ASCP) and the following job description is posted on our university website. Although MS degree was put as the required qualification, a bachelors degree will be closely considered. Bozeman is located 90 miles north of Yellowstone National Park, and is a wonderful place to live, ski, fish, hike, climb, mountain bike, hunt, raise a family, and enjoy beautiful mountains surrounding the valley. Please read the following job description and pay close attention to what is written in bold letters. Research Associate of Mucosal Immunology - Pascual Lab , Veterinary Molecular Biology , Montana State University, Bozeman MT 59717 Search Number: 1035-3 Required Qualifications: Master's of Science degree, preferably in Immunology, Microbiology, Biology, or closely related field. Preferred Qualifications: HT or HTL(ASCP) registered or registry eligible. Microbiology experience. Experience with rodent tissues. Successful research experience Proficiency in operation and maintenance of automated tissue processor, embedding center, microtome, and cryostats. Proficiency in research tissue cryotomy, including snap freezing and cryosectioning tissues. Extensive experience working with mice or related small animals Ability to design experiments and interpret data. Demonstrated written communication skills $32,000- $38,000 per year depending upon experience, education and qualifications. Duties and Responsibilities include: 1. Test existing and new therapeutics to treat autoimmune diseases such as experimental autoimmune encephalitomyelitis (EAE) and collagen-induced arthritis (CIA). 2. Process necropsy and biopsy samples using routine or special fixation, dehydration, clearing, embedding, and microtomy for routine H&E. 3. Perform special staining, immunohistochemical and immunofluorescent staining, and enzyme histochemistry to determine the involved cell types in the biopsied samples. 4. Possess ability to develop new assays to detect novel cytokines in tissue samples. 5. Possess the ability to interpret the histological data and provide input into the most effective therapeutic regimen. 6. Consult weekly with the PI on the progression of the project, and the PI will assist in the decision process to meet the designated goals. 7. Meet with visiting scientists to exchange ideas and present data at national forums and departmental seminars. 8. Train and supervise graduate or undergraduate students and other laboratory personnel on cryostat and laboratory equipment use and any histotechniques when needed. In an attempt to develop therapeutics for multiple sclerosis (MS) and Information arthritis, human autoimmune degenerative diseases associated with inflammation and destruction of self tissues by autoreactive T cells, we have discovered that our M cell targeting vector when genetically fused with auto-antigen, exhibits potent anti-inflammatory properties capable of treating inflammatory diseases. One such experimental inflammatory disease, experimental autoimmune encephalomyelitis (EAE), resembles MS, upon immunization with myelin peptides or proteins. EAE is a T cell-dependent disease, and these encephalitogenic T cells secrete Th1-type cytokines, including IFN-? and IL-2, and Th17-type cytokines, including IL-6, IL-17, and IL-21. A number of studies have evaluated whether oral tolerance could be adapted for treating MS but have met with limited success, thus, presenting a major hurdle for implementing treatment of this autoimmune disease. Experimentally, oral tolerance has proven successful for EAE, although it has lagged behind in treatment of MS; however, conceptually, oral tolerance could be an effective means to stimulate anti-inflammatory responses, if appropriately formulated. To enable treatment, we hypothesized that oral administration of our M cell-based therapeutics, when administered to EAE-susceptible mice, will prevent and treat EAE. Likewise, when administered to arthritis-susceptible mice, the same therapeutics can treat collagen-induced arthritis. To test our hypothesis, 1) studies will determine whether the described M cell-based therapeutics can suppresses or bypass inflammation via induction of regulatory T cells or plasmacytoid dendritic cells; 2) studies will determine how these therapeutics operate in treating ongoing EAE or arthritis by conducting adoptive transfer studies; and 3) studies will determine which inflammatory cell pathways are neutralized by treatment with these therapeutics. Thus, from these studies we will learn the specific mechanisms induced by this anti-inflammatory vaccine. Departmental Information: In an attempt to develop therapeutics for multiple sclerosis (MS) and Information arthritis, human autoimmune degenerative diseases associated with inflammation and destruction of self tissues by autoreactive T cells, we have discovered that our M cell targeting vector when genetically fused with auto-antigen, exhibits potent anti-inflammatory properties capable of treating inflammatory diseases. One such experimental inflammatory disease, experimental autoimmune encephalomyelitis (EAE), resembles MS, upon immunization with myelin peptides or proteins. EAE is a T cell-dependent disease, and these encephalitogenic T cells secrete Th1-type cytokines, including IFN-? and IL-2, and Th17-type cytokines, including IL-6, IL-17, and IL-21. A number of studies have evaluated whether oral tolerance could be adapted for treating MS but have met with limited success, thus, presenting a major hurdle for implementing treatment of this autoimmune disease. Experimentally, oral tolerance has proven successful for EAE, although it has lagged behind in treatment of MS; however, conceptually, oral tolerance could be an effective means to stimulate anti-inflammatory responses, if appropriately formulated. To enable treatment, we hypothesized that oral administration of our M cell-based therapeutics, when administered to EAE-susceptible mice, will prevent and treat EAE. Likewise, when administered to arthritis-susceptible mice, the same therapeutics can treat collagen-induced arthritis. To test our hypothesis, 1) studies will determine whether the described M cell-based therapeutics can suppresses or bypass inflammation via induction of regulatory T cells or plasmacytoid dendritic cells; 2) studies will determine how these therapeutics operate in treating ongoing EAE or arthritis by conducting adoptive transfer studies; and 3) studies will determine which inflammatory cell pathways are neutralized by treatment with these therapeutics. Thus, from these studies we will learn the specific mechanisms induced by this anti-inflammatory vaccine. The Successful Candidate Will: Good organizational, communication, and interpersonal skills to accomplish designated goals of this project and ability to work with other investigators Application Procedure: Screening of applications will begin on December 21, 2009 and continue until the position is filled. To apply, submit: 1) a letter of application that addresses the required and preferred qualifications listed above; 2) a resume and 3) the contact information of three professional references. Electronic submissions in PDF to Dr. David Pascual, dpascual@montana.edu From mwich <@t> 7thwavelabs.com Wed Dec 2 16:04:57 2009 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Wed Dec 2 16:05:45 2009 Subject: [Histonet] removing coverslips Message-ID: <62A8156F8071C8439080D626DF8C33A65D8E93@wave-mail.7thwave.local> Hello. Can anyone tell me how I might remove coverglass from slides containing GMA sections mounted with "UV mount" (PolySciences)? Thank you. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From araniqkslvr <@t> yahoo.com Wed Dec 2 16:21:24 2009 From: araniqkslvr <@t> yahoo.com (Paula) Date: Wed Dec 2 16:21:29 2009 Subject: [Histonet] Bachelor's Degrees Message-ID: <667858.12191.qm@web30402.mail.mud.yahoo.com> When I first entered the field of histotechnology, I was told you needed on the job training or an Associate's degree for a HT and a Bachelors for?a HTL. Yet so many ads nowadays state you MUST have a B.S. degree in science for a HT position. ? Like I had written earlier in the year, I am trying to get back into the field and one place would train me if I had a B.S. in science (I don't--it's liber arts--long story--but I have 30+ credits in science from my A.A. and other courses here and there), but since I didn't have that specific degree they wouldn't even send in my resume. I did pass the exam and am HT registered. ? What does everyone else think of this? It's just really annoying me. I know it's an employer's market out there, but still... ? Thanks, ? Paula From amosbrooks <@t> gmail.com Wed Dec 2 17:22:59 2009 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Dec 2 17:23:04 2009 Subject: [Histonet] procedure for osmium and potassium chromate? Message-ID: <582736990912021522k45420f25mc500cdea7fc96fc6@mail.gmail.com> Hi, Yes, of course it's dangerous. (OK, granted really dangerous) Histology cannot be done with distilled water alone. Risks are part of the job. Knowledge of the risks and understanding of how to minimize these risks is what makes it possible to do our jobs and not have serious problems. Of course if there is another way of doing something without the hazardous chemicals while still getting the same results, one would be a complete idiot not to use the safer alternative. (This is why we cannot find mercury in hematoxylin formulations anymore same results, safer chemicals) There are some things that cannot be substituted. I have looked but I have not find any way of demonstrating lipids, fats or oils in paraffin sections. (If anyone knows of one please enlighten me!) Sometimes paraffin sections are needed instead of frozen sections. This is one that works. So use of the chemical (under obvious precautions) is justifiable. So because I'm a devil's advocate, the reference can be found in Gretchen Humanson's Animal Tissue Techniques. However the fair warnings given earlier are very right. Reading the MSDS is a must. Also for an interesting read check out the Wikipedia pages: http://en.wikipedia.org/wiki/Osmium_tetroxide http://en.wikipedia.org/wiki/Potassium_dichromate Ok I'm getting off the soapbox before someone kicks it out from under me :-) Amos Message: 11 Date: Wed, 2 Dec 2009 13:15:03 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] procedure for osmium and potassium chromate? To: histonet@pathology.swmed.edu, Liz Chlipala Message-ID: <405109.33036.qm@web65705.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I really would suggest you to find an alternative procedure. Osmium tetroxyde is EXTREMELY dangerous and has to be used under extreme precautions. I don't think the results are worth the effort given the great dangers it poses. I for one would not share the procedure with you. Ren? J. --- On Wed, 12/2/09, Liz Chlipala wrote: From: Liz Chlipala Subject: [Histonet] procedure for osmium and potassium chromate? To: histonet@pathology.swmed.edu Date: Wednesday, December 2, 2009, 3:13 PM I'm trying to find a procedure for staining fat in tissues with osmium and I think potassium dichromate, does anyone out there have this protocol available. I have found it before on the web but for some reason I can't find it this time. Liz From tifei <@t> foxmail.com Wed Dec 2 18:55:28 2009 From: tifei <@t> foxmail.com (TF) Date: Wed Dec 2 18:55:36 2009 Subject: [Histonet] Trophic factors staining on brain sections References: <781386178.405021259714644025.JavaMail.root@sz0151a.westchester.pa.mail.comcast.net>, <88613e830912020258r53e1c401r93d997eb33e25f3d@mail.gmail.com> Message-ID: <200912030855273203148@foxmail.com> Hi all to stain out the trophic factors on brain sections, such as BDNFm, IGF1, NGF, CNTF, GDNF will these molecules "leak" out during staining? so I should not use triton and try to shorten the time of staining? Or I need a long time incubation to stain out the "low" concentration small molecules? thanks! 2009-12-02 TF _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dcojita <@t> tampabay.rr.com Wed Dec 2 19:34:14 2009 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Wed Dec 2 19:34:21 2009 Subject: [Histonet] masson trichrome - question Message-ID: Does anyone know if you can substitute hydrochloric acid for phosphomolybdic when performing a masson trichrome stain? From AnthonyH <@t> chw.edu.au Wed Dec 2 19:38:25 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Dec 2 19:38:37 2009 Subject: [Histonet] masson trichrome - question In-Reply-To: Message-ID: No Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of dcojita@tampabay.rr.com Sent: Thursday, 3 December 2009 12:34 PM To: 'histonet' Subject: [Histonet] masson trichrome - question Does anyone know if you can substitute hydrochloric acid for phosphomolybdic when performing a masson trichrome stain? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From annigyg <@t> gmail.com Wed Dec 2 23:41:07 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed Dec 2 23:41:12 2009 Subject: [Histonet] Cerner Millenium In-Reply-To: References: Message-ID: we go live tomorrow night!!! AP all set up and good to go AbuDhabiAnnie 2009/12/2 Evans, Andria B > Does anyone out there in Histoland use Cerner Millenium in there lab? I > have some questions if you could contact me that would be great. > Thanks! > > Andria B Evans, HTL(ASCP)CM > Anatomic Pathology > York Hospital > 1001 S. George Street > York, PA 17405 > 717-851-5006 > > "You can learn a lot more from listening than you can from talking. Find > someone with whom you don't agree in the slightest and ask them to explain > themselves at length. Then take a seat, shut your mouth, and don't argue > back. It's physically impossible to listen with your mouth open." -John Moe > > "Maturity is accepting imperfections." > > CONFIDENTIALITY NOTICE: > > This email may contain confidential health information that is legally > privileged. This information is intended for the use of the named > recipient(s). The authorized recipient of this information is prohibited > from disclosing this information to any party unless required to do so by > law or regulation and is required to destroy the information after its > stated need has been fulfilled. If you are not the intended recipient, you > are hereby notified that any disclosure, copying, distribution, or action > taken in reliance on the contents of this email is strictly prohibited. If > you receive this e-mail message in error, please notify the sender > immediately to arrange disposition of the information. . > > > ______________________________________________________________________ > This e-mail has been scanned by MCI Managed Email Content Service, using > Skeptic(tm) technology powered by MessageLabs. For more information on MCI's > Managed Email Content Service, visit http://www.mci.com. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From jkiernan <@t> uwo.ca Thu Dec 3 00:01:58 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Dec 3 00:02:03 2009 Subject: [Histonet] removing coverslips In-Reply-To: <62A8156F8071C8439080D626DF8C33A65D8E93@wave-mail.7thwave.local> References: <62A8156F8071C8439080D626DF8C33A65D8E93@wave-mail.7thwave.local> Message-ID: Ask PolySciences. If they make the mounting medium thay must know what dissolves it. Please pass on their reply to histonet@lists.utsouthwestern.edu John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Michele Wich Date: Wednesday, December 2, 2009 17:07 Subject: [Histonet] removing coverslips To: histonet@lists.utsouthwestern.edu > Hello. Can anyone tell me how I might remove coverglass from slides > containing GMA sections mounted with "UV mount" (PolySciences)? > > Thank you. > > > This communication is intended solely for the use of the > addressee and may contain information that is legally > privileged, confidential or exempt from disclosure. If you > are not the intended recipient, please note that any > dissemination, distribution, or copying of this communication is > strictly prohibited. Anyone who receives this message in > error should notify the sender immediately and delete it from > his or her computer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Thu Dec 3 04:27:15 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Dec 3 04:27:28 2009 Subject: [Histonet] masson trichrome - question In-Reply-To: Message-ID: <7EFA3B2DCB9C4A98A29B369C6A641418@HPPav2> No, but you could substitute phosphotungstic acid. It's the metal salt (tungsten, molybdenum) that's required for the stain, not the "acid" part. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of dcojita@tampabay.rr.com Sent: Wednesday, December 02, 2009 8:34 PM To: 'histonet' Subject: [Histonet] masson trichrome - question Does anyone know if you can substitute hydrochloric acid for phosphomolybdic when performing a masson trichrome stain? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Thu Dec 3 07:28:26 2009 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Dec 3 07:28:33 2009 Subject: [Histonet] Florida licensure facts Message-ID: Here is the website. These requirements change all the time, so I'd get the official answer if I were you. Have fun filling out the 16 page application. (You probably think I'm joking, but I'm not.) http://www.doh.state.fl.us/mqa/ClinLab/clp_lic_req.html Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) Licensed by the Florida Department of Health since 12/05/08 From 41dmb41 <@t> gmail.com Thu Dec 3 07:35:43 2009 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Thu Dec 3 07:36:08 2009 Subject: [Histonet] CAP question In-Reply-To: <268877.54205.qm@web110303.mail.gq1.yahoo.com> References: <268877.54205.qm@web110303.mail.gq1.yahoo.com> Message-ID: There has already been a lot of responses to this, but I thought I'd add mine anyway. For all our specimens in-house that are delivered from surgery, we have three identifiers present: name, DOB and MR number. We eliminated the use of SSN (from our reports as well), since we all know how dangerous it is to have your SSN just floating around. Specimens received from outside facilities are a little different, because the outside facility can't possibly label the specimen with our MR number; it's assigned once the specimen arrives at our location. In those instances, just the name and DOB are used. At the time of accession, we do add the in-house pathology case number to all the specimens as well. You might call this overkill, but it helps greatly when we need to pull a specimen later on and it helps cut down on grossing errors. The big thing from CAP is the two identifiers. When you're specifically dealing with outpatient centers where you might not be able to get the medical record number on the container at the time of collection, it seems to me that the best choice is to use name and DOB. Just my 2 cents. Drew On Wed, Dec 2, 2009 at 11:49, Una McGiven wrote: > Hi everyone, > > On the most recent CAP checklist there is a requirement for 2 patient identifiers.? For those labs who are CAP and receive all or a large portion of their cases from outside facilities (=not from your facility), what are you using as the identifiers on your specimen containers?? Name and birthdate?? SS#?? Med record?? Something else? > > Thank you in advance! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sfp1968 <@t> gmail.com Thu Dec 3 08:00:44 2009 From: sfp1968 <@t> gmail.com (Terry OBrien) Date: Thu Dec 3 08:00:49 2009 Subject: [Histonet] Lica CM 3600 Message-ID: Does anyone on the east coast have a Lica CM 3600 Cryomarocut cryostat? Need to see one in operation. Thanks. Terry From rjbuesa <@t> yahoo.com Thu Dec 3 08:08:06 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 3 08:08:10 2009 Subject: [Histonet] masson trichrome - question In-Reply-To: Message-ID: <712079.4120.qm@web65703.mail.ac4.yahoo.com> It is not because it is an "acid". Phosphomolybdic acid? has properties that hydrochloric acid don't have. Short: NO Ren? J. --- On Wed, 12/2/09, dcojita@tampabay.rr.com wrote: From: dcojita@tampabay.rr.com Subject: [Histonet] masson trichrome - question To: "'histonet'" Date: Wednesday, December 2, 2009, 8:34 PM Does anyone know if you can substitute hydrochloric acid for phosphomolybdic when performing a masson trichrome stain? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Thu Dec 3 09:23:56 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Dec 3 09:24:03 2009 Subject: [Histonet] RELIA Histology Job Alert 12/03/09 Here are some exciting opportunities amid the hustle and bustle of the holidays! Message-ID: Hi Histonetters!! I hope everyone is doing well and looking forward to celebrating the holidays with friends and family. I have some exciting opportunities that I want to tell you about. I know we are just entering the hustle and bustle of the holidays but I want to assure you that these clients are very flexible and willing to wait until after the holidays for start dates and in some cases onsite interviews can wait as well. What I am saying is that if you are ready to make a move now great!! If it is something you are considering after the first of the year and one of these opportunities looks intriguing then lets you and I talk now and get the ball rolling that way you can register your interest and we can follow up after the holidays. I would hate for you to lose out by waiting to even hear about a position that might be the perfect job for you at the not so perfect time of the year. All of these positions are permanent positions that offer excellent compensation and benefits and in most cases sign on bonuses or relocation assistance. Here is a list of my newest and most current openings: HISTOLOGY/PATHOLOGY MANAGEMENT WI ? Waukesha AP Team Leader OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital CA ? Central CA ? Pathology Supervisor CA ? Los Angeles ?Histology Supervisor MA ? Cape Cod Pathology Supervisor NC ? Charlotte Histology Manager KS ? Topeka AP Manager HISTOTECHS LA ? Lead IHC Tech ?private lab FL ? Pensacola- Histotech private lab MI ? Jackson ? Grossing Histotech. Private lab TX ? Austin ?Night Shift Histotech TX ? Fort Worth MOHS Tech part time Pathology Assistant NC ? Charlotte PA new grads and experienced welcome to apply If you or anyone you know might be interested in any of these opportunities please contact me - Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. Thanks-Pam Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net < mailto:relia1@earthlink.net> << http://home.earthlink.net/~relia1>> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From mcauliff <@t> umdnj.edu Thu Dec 3 10:14:56 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Dec 3 10:13:00 2009 Subject: [Histonet] procedure for osmium and potassium chromate? In-Reply-To: References: Message-ID: <4B17E400.1090802@umdnj.edu> OK, light microscopy. I think frozen sections of formalin fixed (formalin with calcium) material is the way to go here. Wash out the formalin as it will react with the dicrhomate then "fix" with Os or Os-dichromate. Altman's fix is equal parts of 2% aqueous osmium and 5% aqueous potassium dichromate. There are many variations on this mix, I doubt if concentration matters much. Fix sections on slides for an hour or two, wash well. I have fixed mouse kidney with osmium-dichromate for paraffin embedding. The tissue is VERY brittle and hard to cut. I would not expect good results. Geoff Liz Chlipala wrote: > Light Micro. I have done this before and it turned out great on some > mouse muscle, but I misplaced my procedure. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > PO Box 18592 > Boulder, Colorado 80308 > office (303) 682-3949 > fax (303) 682-9060 > www.premierlab.com > > > Ship to Address: > 1567 Skyway Drive, Unit E > Longmont, Colorado 80504 > > -----Original Message----- > From: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] > Sent: Wednesday, December 02, 2009 1:41 PM > To: Liz Chlipala > Cc: histonet@pathology.swmed.edu > Subject: Re: [Histonet] procedure for osmium and potassium chromate? > > Dalton's Chrome-osmium fix was popular for awhile in the 1950's. You > will need to look in an older methods book. The combo of dichromate and > osmium will not make your safety officer happy. There are many ways to > stain lipids with osmium, alone or mixed with something else. Is this > for EM or LM? > > Geoff > > Liz Chlipala wrote: > >> I'm trying to find a procedure for staining fat in tissues with osmium >> and I think potassium dichromate, does anyone out there have this >> protocol available. I have found it before on the web but for some >> reason I can't find it this time. >> >> >> >> Liz >> >> >> >> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC >> >> Manager >> >> Premier Laboratory, LLC >> >> PO Box 18592 >> >> Boulder, Colorado 80308 >> >> office (303) 682-3949 >> >> fax (303) 682-9060 >> >> www.premierlab.com >> >> >> >> >> >> Ship to Address: >> >> 1567 Skyway Drive, Unit E >> >> Longmont, Colorado 80504 >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From kc <@t> ka-recruiting.com Thu Dec 3 10:15:57 2009 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Thu Dec 3 10:15:57 2009 Subject: [Histonet] Histology Supervisor Job Message-ID: <488342117.1259856957444.JavaMail.cfservice@webserver53> Dear Fellow Histonet Subscribers, I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have clients across the country. We are completely free of charge to candidates and are currently working on numerous Histology positions. Our clients often assist with relocation expenses. One position in particular I want to tell you about is a 1st shift Histology Supervisor job with one of the top ranked teaching hospitals in the country. Located in New York City, this hospital is an award winning facility that's consistently ranked in the top 10% in the annual "Best Hospital" issue of US News & World Report. Requirements for the position include: BS in Medical Technology (or related science), HT or HTL ASCP certification and NY State License. Must have at least 5 years of experience working as a Histotechnologist in a clinical lab setting with recent supervisory experience. This position offers a competitive compensation package with outstanding benefits including retirement package. Below is a list of some of the other Histology opportunities we are currently working on. 1) New York City - Histotechnologist -3rd shift 2) Oklahoma - Histotechnologist - 1st shift 3) Georgia (SE part of the state) - Histology Supervisor - 1st shift 4) Georgia (SE part of the state) - Histotechnologist - 2 positions open 5) California (Bay Area) - Pathology Manager - 1st shift 6) California (Los Angeles) - Histology Supervisor - 1st shift 7) California (san Diego) - Sr. Histotechnologist - 1st shift 8) Massachusetts (Boston) - Histotechnologist - 1st shift Contact me if you're interested in learning more about any of these opportunities or if you'd like to be considered for future positions that come up with our clients. We work on positions at all levels and cover the entire US. Please also send me an updated resume and let me know what the best way to reach you is. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From mtitford <@t> aol.com Thu Dec 3 10:31:37 2009 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Thu Dec 3 10:31:51 2009 Subject: [Histonet] Slide Crusher Message-ID: <8CC424D3809CC42-F90-651F@webmail-m028.sysops.aol.com> Debbie Boyd in Virginia asks about slide crushers: At the National Society meeting in Birmingham recently, a company called Creative Waste Solutions displayed a slide crusher. It grinds them up and the slide labels too until it is just like very roughly ground sugar. The instrument costs several thousand dollars, but, better still, you can rent it. They will rent you the instrument, and give you bags and boxes to put the ground up slides in. Then I guess it is off to the landfill. Their telephone number is (888) 795 8300 (www.slydeater.com). Jim Maynard, a histotechnologist, represents them in Florida. his number is (352) 978 9197 (I will need to rent one when I finally retire and I have to empty my office!) Mike Titford USA Pathology Mobile AL USA From srishan <@t> mail.holyname.org Thu Dec 3 10:48:08 2009 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Thu Dec 3 10:49:10 2009 Subject: [Histonet] regarding using neg controls for H.pylori Message-ID: Good Morning, I am sure this question has popped up before, but, when you are running H.pylori by immunohistochemistry do you have to run a patient pos and patient neg for each patient. If you have 10 patients do the run consist of 20 slides each patient having a pos and neg. What does the CAP suggest us to do.? We have a debate going regarding this issue and I would like clarification. I understand when we do 2 slides each there is only one billable item and the neg is using reagents but cannot be reimbursed. Thanks Mala Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare , 2009 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From lblazek <@t> digestivespecialists.com Thu Dec 3 10:49:31 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Dec 3 10:51:05 2009 Subject: [Histonet] regarding using neg controls for H.pylori In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39098BE4D9E9@IBMB7Exchange.digestivespecialists.com> Mala, We put a positive control on the top portion of the patient slide. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of srishan@mail.holyname.org Sent: Thursday, December 03, 2009 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] regarding using neg controls for H.pylori Good Morning, I am sure this question has popped up before, but, when you are running H.pylori by immunohistochemistry do you have to run a patient pos and patient neg for each patient. If you have 10 patients do the run consist of 20 slides each patient having a pos and neg. What does the CAP suggest us to do.? We have a debate going regarding this issue and I would like clarification. I understand when we do 2 slides each there is only one billable item and the neg is using reagents but cannot be reimbursed. Thanks Mala Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare , 2009 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Dec 3 10:58:19 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 3 10:58:23 2009 Subject: [Histonet] regarding using neg controls for H.pylori In-Reply-To: Message-ID: <437187.44481.qm@web65707.mail.ac4.yahoo.com> When I had a series of cases all for the same Ab, I used to run 1 positive and 1 negative for the whole series. All the cases and those 2 controls were put together for the pathologist to review. Later those 2 controls were filed separate with the date they were run. I never had a problem with CAP by doing that, especially because I was using and auto stainer that accounted for reliability of the run (no personal mistakes). Ren? J. --- On Thu, 12/3/09, srishan@mail.holyname.org wrote: From: srishan@mail.holyname.org Subject: [Histonet] regarding using neg controls for H.pylori To: histonet@lists.utsouthwestern.edu Date: Thursday, December 3, 2009, 11:48 AM Good Morning, I am sure this question has popped up before, but,? when you are running H.pylori by immunohistochemistry do you have to run a patient pos and patient neg for each patient.? If you have 10 patients do the run consist of 20 slides each patient having a pos and neg. What does the CAP suggest us to do.?? We have a debate going regarding this issue and I would like clarification.? I understand when we do 2 slides each? there is only one billable item and the neg is using reagents but cannot be reimbursed. Thanks Mala Holy Name Hospital? is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by? Modern Healthcare , 2009 Best Places to Work in New Jersey,? NJBIZ? - 2006, 2007, 2008 & 2009? Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007? Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008? Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009? Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system.? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Donadio <@t> bhcpns.org Thu Dec 3 11:02:31 2009 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Thu Dec 3 11:02:57 2009 Subject: [Histonet] regarding using neg controls for H.pylori In-Reply-To: Message-ID: Here we run a pos control which is on each patient slide and one Neg control for every block such as "A, B, Etc" I believe this is the CAP recommendation You can look at all the specific guidelines by going to the CAP website and looking at questions ANP.22550 and ANP.22570 Hope this helps Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 srishan@mail.holyname.org Sent by: histonet-bounces@lists.utsouthwestern.edu 12/03/2009 10:48 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] regarding using neg controls for H.pylori Good Morning, I am sure this question has popped up before, but, when you are running H.pylori by immunohistochemistry do you have to run a patient pos and patient neg for each patient. If you have 10 patients do the run consist of 20 slides each patient having a pos and neg. What does the CAP suggest us to do.? We have a debate going regarding this issue and I would like clarification. I understand when we do 2 slides each there is only one billable item and the neg is using reagents but cannot be reimbursed. Thanks Mala Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare , 2009 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From Samuel_Perry <@t> DFCI.HARVARD.EDU Thu Dec 3 11:30:47 2009 From: Samuel_Perry <@t> DFCI.HARVARD.EDU (Perry, Samuel) Date: Thu Dec 3 11:30:52 2009 Subject: [Histonet] IF colocalization with 2 rabbit antibodies Message-ID: Hi All, I know this might be a dumb question. Has anyone been able to perform CO-IF staining with two rabbit antibodies with confidence in specificity of the signals? I am worried that using the same specie antibody with cause specificity issues. I'm interested in staining p-S6RP and p-44/42 (two rabbit mAb from cell signaling) on the same section of mouse tissue. Any suggestions would be very helpful even if people have tried and failed; that would be good to know. Thanks! Sam Perry Research Tech DFCI The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From KMB01 <@t> grh.org Thu Dec 3 11:35:53 2009 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Thu Dec 3 11:37:23 2009 Subject: [Histonet] FORMALDEHYDE Message-ID: Good Morning Histo Land, I was just informed that on my last test sample for formaldehyde vapors was very high. Right now I have to wear a respirator while grossing and assisting grossing. We are looking at why the Mopec grossing station is not working in fact we have not had really good results since we put it in. I'm wondering if anyone else is having the same problem or know of a better solution. Our grossing station is the one that is self contained with filters. Also is anyone using with good result a formalin substitute? If so which one? Thanks and Have a great day, Kathy Gorham, H.T. Grande Ronde Hospital La Grande Oregon GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS GRH Mission We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. From rjbuesa <@t> yahoo.com Thu Dec 3 11:41:54 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 3 11:41:58 2009 Subject: [Histonet] FORMALDEHYDE In-Reply-To: Message-ID: <590798.17691.qm@web65716.mail.ac4.yahoo.com> Regardless of what others may be experiencing with a similar station, I think that you should check the filters of yours and the air circulation. As to formalin substitutes I am sending you under separate cover an article on the subject. Ren? J. --- On Thu, 12/3/09, Kathy M. Gorham wrote: From: Kathy M. Gorham Subject: [Histonet] FORMALDEHYDE To: histonet@lists.utsouthwestern.edu Date: Thursday, December 3, 2009, 12:35 PM Good Morning Histo Land, I was just informed that on my last test sample for formaldehyde vapors was very high.? Right now I have to wear a respirator while grossing and assisting grossing.? We are looking at why the Mopec grossing station is not working in fact we have not had really good results since we put it in.? I'm wondering if anyone else is having the same problem or know of a better solution.? Our grossing station is the one that is self contained with filters. Also is anyone using with good result a formalin substitute? If so which one? Thanks and Have a great day, Kathy Gorham, H.T. Grande Ronde Hospital La Grande Oregon GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS GRH Mission We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission.? If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Thu Dec 3 11:57:22 2009 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Thu Dec 3 11:57:35 2009 Subject: [Histonet] RE: FORMALDEHYDE In-Reply-To: References: Message-ID: I have just purchased two Mopec MB600 grossing stations and we do not have this problem. Our pathologist actually rave about the unit. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Thursday, December 03, 2009 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FORMALDEHYDE Good Morning Histo Land, I was just informed that on my last test sample for formaldehyde vapors was very high. Right now I have to wear a respirator while grossing and assisting grossing. We are looking at why the Mopec grossing station is not working in fact we have not had really good results since we put it in. I'm wondering if anyone else is having the same problem or know of a better solution. Our grossing station is the one that is self contained with filters. Also is anyone using with good result a formalin substitute? If so which one? Thanks and Have a great day, Kathy Gorham, H.T. Grande Ronde Hospital La Grande Oregon GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS GRH Mission We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From cliffberger <@t> mac.com Thu Dec 3 11:59:26 2009 From: cliffberger <@t> mac.com (Cliff Berger) Date: Thu Dec 3 11:59:44 2009 Subject: [Histonet] FORMALDEHYDE In-Reply-To: Message-ID: Formaldehyde fumes require filters which have been treated with Potassium Permanganate. Normal activated charcoal filter will not trap formaldehyde fumes. -- Best regards! Cliff Berger Decal Chemical Corp 1-800-428-5856 - Office 914-588-5019 - Cell On 12/3/09 12:35 PM, "Kathy M. Gorham" wrote: > Good Morning Histo Land, I was just informed that on my last test sample > for formaldehyde vapors was very high. Right now I have to wear a > respirator while grossing and assisting grossing. We are looking at why > the Mopec grossing station is not working in fact we have not had really > good results since we put it in. I'm wondering if anyone else is having > the same problem or know of a better solution. Our grossing station is > the one that is self contained with filters. Also is anyone using with > good result a formalin substitute? If so which one? > Thanks and Have a great day, > > Kathy Gorham, H.T. > Grande Ronde Hospital > La Grande Oregon > > > GRH National Recognition > Outstanding Rural Health Organization of 2009 awarded by NRHA > Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP > Leader in Innovative Excellence 2009 awarded by the OAHHS > > GRH Mission > We will ensure ACCESS to superior QUALITY, affordable health care for all > those in need of our services, including underserved populations within our > communities. > > > GRH Confidentiality Notice > This e-mail and any attached documents are for the intended recipient/s only > and should be protected against viewing by unauthorized persons. The > information > herein may have been disclosed from records whose confidentiality is protected > by Federal and State Law. Federal regulations prohibit further distribution or > copying of this information without permission. If you received this e-mail > transmission in error, please notify the sender immediately to arrange for > return > or destruction of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michelle-griffin <@t> uiowa.edu Thu Dec 3 12:15:19 2009 From: michelle-griffin <@t> uiowa.edu (Griffin, Michelle A) Date: Thu Dec 3 12:15:43 2009 Subject: [Histonet] RE:Formaldehyde In-Reply-To: <200912031801.nB3I1GAt018966@star.its.uiowa.edu> References: <200912031801.nB3I1GAt018966@star.its.uiowa.edu> Message-ID: Our lab tried the self contained grossing station with filters (bench-top) and we had troubles with it adequately filtering formalin fumes (even though the spec sheet stated that was what it was for...from MOPEC). We ended up installing a fume hood to use for gross prosection, waste discard, etc. In our situation and experience, the fume hood was the best way to go, especially when we need to use extra precautions for tissue fixing/staining/etc. Michelle A. Griffin Research Assistant II University of Iowa Department of Pathology 200 Hawkins Drive 143 Medical Research Center Iowa City, IA 52242 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Thursday, December 03, 2009 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FORMALDEHYDE Good Morning Histo Land, I was just informed that on my last test sample for formaldehyde vapors was very high. Right now I have to wear a respirator while grossing and assisting grossing. We are looking at why the Mopec grossing station is not working in fact we have not had really good results since we put it in. I'm wondering if anyone else is having the same problem or know of a better solution. Our grossing station is the one that is self contained with filters. Also is anyone using with good result a formalin substitute? If so which one? Thanks and Have a great day, Kathy Gorham, H.T. Grande Ronde Hospital La Grande Oregon GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS GRH Mission We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ From BFicher <@t> chomp.org Thu Dec 3 12:23:41 2009 From: BFicher <@t> chomp.org (Fischer, R. B) Date: Thu Dec 3 12:24:10 2009 Subject: [Histonet] FORMALDEHYDE In-Reply-To: References: Message-ID: I suggest having your bio-med or engineering department check the generator exhaust belts that power the ventilation to see if they have worn, slipped or have broken... R.Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box HH Monterey Ca. 93942 831-625-4791 Fax: 831-6583683 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Thursday, December 03, 2009 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FORMALDEHYDE Good Morning Histo Land, I was just informed that on my last test sample for formaldehyde vapors was very high. Right now I have to wear a respirator while grossing and assisting grossing. We are looking at why the Mopec grossing station is not working in fact we have not had really good results since we put it in. I'm wondering if anyone else is having the same problem or know of a better solution. Our grossing station is the one that is self contained with filters. Also is anyone using with good result a formalin substitute? If so which one? Thanks and Have a great day, Kathy Gorham, H.T. Grande Ronde Hospital La Grande Oregon GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS GRH Mission We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. From HornHV <@t> archildrens.org Thu Dec 3 12:24:48 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Dec 3 12:24:53 2009 Subject: [Histonet] regarding using neg controls for H.pylori In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D835C0@EMAIL.archildrens.org> We do this as well. Each block has a pt. positive slide and a pt. negative slide for that block. If multiple antibodies are ordered on the same block, we usually will have one negative mouse and one negative rabbit for the block. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Thursday, December 03, 2009 11:03 AM To: srishan@mail.holyname.org Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] regarding using neg controls for H.pylori Here we run a pos control which is on each patient slide and one Neg control for every block such as "A, B, Etc" I believe this is the CAP recommendation You can look at all the specific guidelines by going to the CAP website and looking at questions ANP.22550 and ANP.22570 Hope this helps Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 srishan@mail.holyname.org Sent by: histonet-bounces@lists.utsouthwestern.edu 12/03/2009 10:48 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] regarding using neg controls for H.pylori Good Morning, I am sure this question has popped up before, but, when you are running H.pylori by immunohistochemistry do you have to run a patient pos and patient neg for each patient. If you have 10 patients do the run consist of 20 slides each patient having a pos and neg. What does the CAP suggest us to do.? We have a debate going regarding this issue and I would like clarification. I understand when we do 2 slides each there is only one billable item and the neg is using reagents but cannot be reimbursed. Thanks Mala Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare , 2009 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From TJJ <@t> stowers.org Thu Dec 3 12:49:29 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Thu Dec 3 12:49:39 2009 Subject: [Histonet] Re: IF colocalization with 2 rabbit antibodies Message-ID: Hi Sam, You asked if it was possible to do colocalization IF staining using two rabbit primary antibodies, one for p-S6RP and one for p-44/42. You will have to be the one to determine if it's possible to do this on your samples. In theory, everything is possible. ;-) First you'll need to optimize each of the antibodies singly in your mouse tissue, using proper negative controls substituting normal rabbit IgG and determining if you are indeed getting specific signal from each antibody. Once you have done that and you know what the antibody expression is for each one, you can move to designing the protocol for double staining. There are a variety of ways to accomplish this you can find doing a literature search. Probably the easiest method is to use a sequential IHC protocol where you treat with rabbit primary antibody #1, Fl-labeled secondary anti-rabbit #1, and then close any open binding sites using an incubation of unlabeled monovalent Fab fragments (http://www.jacksonimmuno.com/Catalog/monovalent.asp) or normal rabbit serum. Then you move on to rabbit primary antibody #2, and then Fl-labeled secondary anti-rabbit antibody #2. Proper controls of this are critical to ensure what you are seeing is specific. Ideally, you will need 2 controls where you substitute normal rabbit IgG for one of the rabbit primaries but not the other (should end up looking EXACTLY like your single stain results), and another that substitutes normal rabbit IgG for both the primaries in the technique just as outlined above (should be totally negative). If this isn't clear, don't hesitate to contact me directly. Another wonderful contact is Chris van der Loos, he does this all the time and makes it look easy. Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research Kansas City, MO From mroark <@t> sfmc.net Thu Dec 3 13:22:10 2009 From: mroark <@t> sfmc.net (Matthew Roark) Date: Thu Dec 3 13:22:56 2009 Subject: [Histonet] Control Slides/blocks/tissue Message-ID: <4B17BB830200001100075783@email_gateway.sfmc.net> Hello all, I'm looking for positive control slides for Girardia and Cryptosporidia. If you know of a company that has slides or blocks available please let me know. Thanks!!!!!! Matthew Roark Histology Specialist- B.S,HT/HTL(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 From jeri <@t> opssearchgroup.com Thu Dec 3 13:48:31 2009 From: jeri <@t> opssearchgroup.com (jeri@opssearchgroup.com) Date: Thu Dec 3 13:48:51 2009 Subject: [Histonet] (no subject) Message-ID: Histonetters, I am hoping you folks can provide an opinion. I am assisting an individual who has a Biology degree, HTL certification and for the past 3 years worked in the histology section of a private lab where they cut, embedded and stained animal tissues. He would like to transition to a hospital lab. Is there any reason his skill set and knowledge would be incompatible with his desired new ambition? Jeri Vitello From christiegowan <@t> msn.com Thu Dec 3 14:17:16 2009 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Thu Dec 3 14:17:23 2009 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: His skill set should be fine for any lab setting. I have worked in hospital labs and animal only private labs and had no problems. Christie > From: jeri@opssearchgroup.com > To: histonet@lists.utsouthwestern.edu > Date: Thu, 3 Dec 2009 14:48:31 -0500 > Subject: [Histonet] (no subject) > > Histonetters, > > I am hoping you folks can provide an opinion. I am assisting an individual who has a Biology degree, HTL certification and for the past 3 years worked in the histology section of a private lab where they cut, embedded and stained animal tissues. He would like to transition to a hospital lab. Is there any reason his skill set and knowledge would be incompatible with his desired new ambition? > > Jeri Vitello > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Thu Dec 3 14:18:30 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Thu Dec 3 14:18:34 2009 Subject: [Histonet] (no subject) References: Message-ID: <90354A475B420441B2A0396E5008D4965E30AB@copc-sbs.COPC.local> Hi Jeri, He should be fine. Passing the HTL demonstrates considerable knowledge which is applicable to the clinical (human) lab world. Also, I'm of the opinion that animal tissue - in general - is more difficult to section than human tissue. Not actually knowing the person - which makes a difference - and with the information you've provided, again, he should be fine. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jeri@opssearchgroup.com Sent: Thursday, December 03, 2009 11:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Histonetters, I am hoping you folks can provide an opinion. I am assisting an individual who has a Biology degree, HTL certification and for the past 3 years worked in the histology section of a private lab where they cut, embedded and stained animal tissues. He would like to transition to a hospital lab. Is there any reason his skill set and knowledge would be incompatible with his desired new ambition? Jeri Vitello _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Dec 3 15:03:22 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 3 15:03:28 2009 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: <930906.3692.qm@web65703.mail.ac4.yahoo.com> Absolutely not. As a matter of fact animal histotechnique is more challenging than human. Ren? J. --- On Thu, 12/3/09, jeri@opssearchgroup.com wrote: From: jeri@opssearchgroup.com Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Date: Thursday, December 3, 2009, 2:48 PM Histonetters, I am hoping you folks can provide an opinion.? I am assisting an individual who has a Biology degree, HTL certification and for the past 3 years worked in the histology section of a private lab where they cut, embedded and stained animal tissues. He would like to transition to a hospital lab.? Is there any reason his skill set and knowledge would be incompatible with his desired new ambition? Jeri Vitello _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLunetta <@t> luhcares.org Thu Dec 3 15:18:18 2009 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Thu Dec 3 15:18:36 2009 Subject: [Histonet] FORMALDEHYDE Message-ID: <4B17C8AA020000A80003B83B@gw4.luh.luhcares.org> We have two Mopec grossing stations and they are great. While ours are hooked up to the hospital air system have you checked to see if the in-flow of air up tot he filters is sufficiant?. Mopec also has great customer service so I would call you rep and have them come out to see what is going on. Matt L HT (ASCP) Longmont United Hospital Message: 1 Date: Thu, 3 Dec 2009 11:57:22 -0600 From: "Nails, Felton" Subject: [Histonet] RE: FORMALDEHYDE To: "'Kathy M. Gorham'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii I have just purchased two Mopec MB600 grossing stations and we do not have this problem. Our pathologist actually rave about the unit. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Thursday, December 03, 2009 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FORMALDEHYDE Good Morning Histo Land, I was just informed that on my last test sample for formaldehyde vapors was very high. Right now I have to wear a respirator while grossing and assisting grossing. We are looking at why the Mopec grossing station is not working in fact we have not had really good results since we put it in. I'm wondering if anyone else is having the same problem or know of a better solution. Our grossing station is the one that is self contained with filters. Also is anyone using with good result a formalin substitute? If so which one? Thanks and Have a great day, Kathy Gorham, H.T. Grande Ronde Hospital La Grande Oregon GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS GRH Mission We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ ------------------------------ Message: 2 Date: Thu, 03 Dec 2009 12:59:26 -0500 From: Cliff Berger Subject: Re: [Histonet] FORMALDEHYDE To: "Kathy M. Gorham" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Formaldehyde fumes require filters which have been treated with Potassium Permanganate. Normal activated charcoal filter will not trap formaldehyde fumes. -- Best regards! Cliff Berger Decal Chemical Corp 1-800-428-5856 - Office 914-588-5019 - Cell On 12/3/09 12:35 PM, "Kathy M. Gorham" wrote: > Good Morning Histo Land, I was just informed that on my last test sample > for formaldehyde vapors was very high. Right now I have to wear a > respirator while grossing and assisting grossing. We are looking at why > the Mopec grossing station is not working in fact we have not had really > good results since we put it in. I'm wondering if anyone else is having > the same problem or know of a better solution. Our grossing station is > the one that is self contained with filters. Also is anyone using with > good result a formalin substitute? If so which one? > Thanks and Have a great day, > > Kathy Gorham, H.T. > Grande Ronde Hospital > La Grande Oregon > > > GRH National Recognition > Outstanding Rural Health Organization of 2009 awarded by NRHA > Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP > Leader in Innovative Excellence 2009 awarded by the OAHHS > > GRH Mission > We will ensure ACCESS to superior QUALITY, affordable health care for all > those in need of our services, including underserved populations within our > communities. > > > GRH Confidentiality Notice > This e-mail and any attached documents are for the intended recipient/s only > and should be protected against viewing by unauthorized persons. The > information > herein may have been disclosed from records whose confidentiality is protected > by Federal and State Law. Federal regulations prohibit further distribution or > copying of this information without permission. If you received this e-mail > transmission in error, please notify the sender immediately to arrange for > return > or destruction of this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 73, Issue 6 *************************************** From JPeters <@t> bostwicklaboratories.com Thu Dec 3 15:36:09 2009 From: JPeters <@t> bostwicklaboratories.com (Justin Peters) Date: Thu Dec 3 15:36:15 2009 Subject: [Histonet] buffer protocols Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F07C1D626@mail1.BOSTWICK.COM> Can someone help me with a protocol for making citrate buffer (pH 6.0) for IHC antigen retrieval? Justin Peters, HTL (ASCP) IHC Supervisor Bostwick Laboratories(tm) For Absolute Confidence(r) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 ext. 1831 Cell: (804) 822-6084 Email: jpeters@bostwicklaboratories.com From shive003 <@t> umn.edu Thu Dec 3 15:41:57 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Dec 3 15:42:12 2009 Subject: [Histonet] buffer protocols References: <24D22DE9E488AA43BF92A4389F2DDB1F07C1D626@mail1.BOSTWICK.COM> Message-ID: <03B33DF6F8FD45F69114A4F4E2FD57B4@auxs.umn.edu> http://www.ihcworld.com/_protocols/epitope_retrieval/citrate_buffer.htm Jan Shivers ----- Original Message ----- From: "Justin Peters" To: Sent: Thursday, December 03, 2009 3:36 PM Subject: [Histonet] buffer protocols Can someone help me with a protocol for making citrate buffer (pH 6.0) for IHC antigen retrieval? Justin Peters, HTL (ASCP) IHC Supervisor Bostwick Laboratories(tm) For Absolute Confidence(r) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 ext. 1831 Cell: (804) 822-6084 Email: jpeters@bostwicklaboratories.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brewerd <@t> nacmem.org Thu Dec 3 16:27:09 2009 From: brewerd <@t> nacmem.org (Dana L Brewer) Date: Thu Dec 3 16:25:41 2009 Subject: [Histonet] RE: FORMALDEHYDE In-Reply-To: References: Message-ID: We have also experienced an increase in formalin vapor levels since the installation of our Thermo Grossing Station. It is self-contained and utilizes potassium permanganate filters for formalin neutralization. Has anyone had any quality control issues with these filters? Our last set blew potassium permanganate dust particles all over the lab and everything / everyone in it. Dana Brewer -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Thursday, December 03, 2009 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FORMALDEHYDE Good Morning Histo Land, I was just informed that on my last test sample for formaldehyde vapors was very high. Right now I have to wear a respirator while grossing and assisting grossing. We are looking at why the Mopec grossing station is not working in fact we have not had really good results since we put it in. I'm wondering if anyone else is having the same problem or know of a better solution. Our grossing station is the one that is self contained with filters. Also is anyone using with good result a formalin substitute? If so which one? Thanks and Have a great day, Kathy Gorham, H.T. Grande Ronde Hospital La Grande Oregon GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS GRH Mission We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is intended only for the use of the individual(s) to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, please delete/destroy all electronic and hard copies of this e-mail immediately and notify the sender that the e-mail was sent in error. From rsrichmond <@t> gmail.com Thu Dec 3 22:21:35 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Dec 3 22:21:38 2009 Subject: [Histonet] what book to study for registry? Message-ID: I'm working with a thirtysomething man with a degree in chemistry and a good bit of experience under his belt, who in these bad times wound up signing on as a histech with no experience. Trained by an elderly minimally competent tech, he's rapidly blossomed - is making superb slides and is really interested in histotechnology. He's appalled by the minimal knowledge of science of his co-workers. With the base ulterior motive of trying to keep him from taking off when times get better, I've been strongly encouraging him to take the registry exam when he becomes eligible in a few more months. I'll try to work with him as much as I can. My question to you all is - what book should he be studying? Is the exam still based on Freida Carson's book (2nd ed) which we have, or is there a more recent book we should get? Bob Richmond Samurai Pathologist Knoxville TN From annigyg <@t> gmail.com Fri Dec 4 00:46:57 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Fri Dec 4 00:47:04 2009 Subject: [Histonet] FORMALDEHYDE In-Reply-To: <4B17C8AA020000A80003B83B@gw4.luh.luhcares.org> References: <4B17C8AA020000A80003B83B@gw4.luh.luhcares.org> Message-ID: selfcontained with filters is the probable problem - with formalin its always best to have filters and OUTSIDE venting we have the shandon gross station, vented out of the building with 2 filters which we change regularly lab air is fresh and clean every time we test 2009/12/4 Matthew Lunetta > We have two Mopec grossing stations and they are great. While ours are > hooked up to the hospital air system have you checked to see if the in-flow > of air up tot he filters is sufficiant?. > > Mopec also has great customer service so I would call you rep and have them > come out to see what is going on. > > Matt L > HT (ASCP) > Longmont United Hospital > > Message: 1 > Date: Thu, 3 Dec 2009 11:57:22 -0600 > From: "Nails, Felton" > Subject: [Histonet] RE: FORMALDEHYDE > To: "'Kathy M. Gorham'" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset=us-ascii > > I have just purchased two Mopec MB600 grossing stations and we do not have > this problem. Our pathologist actually rave about the unit. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham > Sent: Thursday, December 03, 2009 11:36 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] FORMALDEHYDE > > Good Morning Histo Land, I was just informed that on my last test sample > for formaldehyde vapors was very high. Right now I have to wear a respirator > while grossing and assisting grossing. We are looking at why the Mopec > grossing station is not working in fact we have not had really good results > since we put it in. I'm wondering if anyone else is having the same problem > or know of a better solution. Our grossing station is the one that is self > contained with filters. Also is anyone using with good result a formalin > substitute? If so which one? > Thanks and Have a great day, > > Kathy Gorham, H.T. > Grande Ronde Hospital > La Grande Oregon > > > GRH National Recognition > Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard > Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in > Innovative Excellence 2009 awarded by the OAHHS > > GRH Mission > We will ensure ACCESS to superior QUALITY, affordable health care for all > those in need of our services, including underserved populations within our > communities. > > > GRH Confidentiality Notice > This e-mail and any attached documents are for the intended recipient/s > only and should be protected against viewing by unauthorized persons. The > information herein may have been disclosed from records whose > confidentiality is protected by Federal and State Law. Federal regulations > prohibit further distribution or copying of this information without > permission. If you received this e-mail transmission in error, please notify > the sender immediately to arrange for return or destruction of this > information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination, or > copying of this e-mail and its attachments, if any, or > the information contained herein is prohibited. If you > have received this e-mail in error, please immediately > notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ============================================================ > > > > ------------------------------ > > Message: 2 > Date: Thu, 03 Dec 2009 12:59:26 -0500 > From: Cliff Berger > Subject: Re: [Histonet] FORMALDEHYDE > To: "Kathy M. Gorham" , > histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > Formaldehyde fumes require filters which have been treated with Potassium > Permanganate. Normal activated charcoal filter will not trap formaldehyde > fumes. > > > -- > Best regards! > > > Cliff Berger > Decal Chemical Corp > 1-800-428-5856 - Office > 914-588-5019 - Cell > > > > On 12/3/09 12:35 PM, "Kathy M. Gorham" wrote: > > > Good Morning Histo Land, I was just informed that on my last test sample > > for formaldehyde vapors was very high. Right now I have to wear a > > respirator while grossing and assisting grossing. We are looking at why > > the Mopec grossing station is not working in fact we have not had really > > good results since we put it in. I'm wondering if anyone else is having > > the same problem or know of a better solution. Our grossing station is > > the one that is self contained with filters. Also is anyone using with > > good result a formalin substitute? If so which one? > > Thanks and Have a great day, > > > > Kathy Gorham, H.T. > > Grande Ronde Hospital > > La Grande Oregon > > > > > > GRH National Recognition > > Outstanding Rural Health Organization of 2009 awarded by NRHA > > Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP > > Leader in Innovative Excellence 2009 awarded by the OAHHS > > > > GRH Mission > > We will ensure ACCESS to superior QUALITY, affordable health care for all > > those in need of our services, including underserved populations within > our > > communities. > > > > > > GRH Confidentiality Notice > > This e-mail and any attached documents are for the intended recipient/s > only > > and should be protected against viewing by unauthorized persons. The > > information > > herein may have been disclosed from records whose confidentiality is > protected > > by Federal and State Law. Federal regulations prohibit further > distribution or > > copying of this information without permission. If you received this > e-mail > > transmission in error, please notify the sender immediately to arrange > for > > return > > or destruction of this information. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 73, Issue 6 > *************************************** > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From ian.montgomery <@t> bio.gla.ac.uk Fri Dec 4 02:16:13 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Dec 4 02:18:54 2009 Subject: FW: [Histonet] what book to study for registry? Message-ID: Robert, Apart from what is recommended for the registry exam I would strongly recommend he reads the latest edition of John Kiernan's text, Histological and Histochemical Methods. Being available in paperback form it will not make a large dent in his wallet. This text is packed with information on technique and the background to it, plus, it's well written and easily read. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: 04 December 2009 04:22 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] what book to study for registry? I'm working with a thirtysomething man with a degree in chemistry and a good bit of experience under his belt, who in these bad times wound up signing on as a histech with no experience. Trained by an elderly minimally competent tech, he's rapidly blossomed - is making superb slides and is really interested in histotechnology. He's appalled by the minimal knowledge of science of his co-workers. With the base ulterior motive of trying to keep him from taking off when times get better, I've been strongly encouraging him to take the registry exam when he becomes eligible in a few more months. I'll try to work with him as much as I can. My question to you all is - what book should he be studying? Is the exam still based on Freida Carson's book (2nd ed) which we have, or is there a more recent book we should get? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Fri Dec 4 05:03:18 2009 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Dec 4 05:03:23 2009 Subject: [Histonet] What book to study for registry? Message-ID: At AFIP we used *Theory and* *Practice of Histotechnology, *by Sheehan and Hrapchak as our primary text. It's available on Amazon.com for around $71.00. We also used the *Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology* edited by Lee Luna for special staining theory. This one might be a little harder to find, although it's been on the bookshelf of any good Histopathology lab I've ever been in. Everyone from my class, who attempted it, passed the ASCP registry on the first try. Lee Luna lives! Jay A. Lundgren, M.S., HTL (ASCP) From kelleydurden <@t> pathology.ufl.edu Fri Dec 4 07:32:43 2009 From: kelleydurden <@t> pathology.ufl.edu (Durden, Kelley) Date: Fri Dec 4 07:33:19 2009 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <92E6B93E0A3D544C87DDDE33E7608AAE0956C7F1@HSC-CMS01.ad.ufl.edu> I completely agree with the other responses you've gotten on this inquiry. Moving from clinical to animal research, initially, I thought I was doing something wrong or had "lost my touch." But upon further instruction from my histo supervisor in my new lab I was able to adjust. So - going in the opposite direction - I think he will welcome the change and do very well. When I do get the opportunity to cut human samples now it's like a "hot knife through butter." Tell your acquaintance to take heart and have confidence in his ability - he'll probably be great! Kelley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jeri@opssearchgroup.com Sent: Thursday, December 03, 2009 2:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Histonetters, I am hoping you folks can provide an opinion. I am assisting an individual who has a Biology degree, HTL certification and for the past 3 years worked in the histology section of a private lab where they cut, embedded and stained animal tissues. He would like to transition to a hospital lab. Is there any reason his skill set and knowledge would be incompatible with his desired new ambition? Jeri Vitello _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Fri Dec 4 07:47:03 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Dec 4 07:48:19 2009 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: There is no problem. I work on both human and animal tissues in the same lab with the same equipment. The only difference is in the accompanying paperwork. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jeri@opssearchgroup.com Sent: Thursday, December 03, 2009 2:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Histonetters, I am hoping you folks can provide an opinion. I am assisting an individual who has a Biology degree, HTL certification and for the past 3 years worked in the histology section of a private lab where they cut, embedded and stained animal tissues. He would like to transition to a hospital lab. Is there any reason his skill set and knowledge would be incompatible with his desired new ambition? Jeri Vitello _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aajl7979 <@t> hotmail.com Fri Dec 4 08:37:05 2009 From: aajl7979 <@t> hotmail.com (Amanda L) Date: Fri Dec 4 08:37:09 2009 Subject: [Histonet] what book to study for registry? In-Reply-To: References: Message-ID: When i took my reg exam i used the carson book and it went well for me. I do know a new edition came out though. Also i went to a class at a regional confrence to help prepare for the exam if you guys have a fax number i would be more than happy to fax you the study material they gave us. It was a big help it lists all fixitaives and stains and what not that you would expect to be on the exam. I can do this for your student on Monday Have a nice weekend Amanda > From: ian.montgomery@bio.gla.ac.uk > To: histonet@lists.utsouthwestern.edu > Date: Fri, 4 Dec 2009 08:16:13 +0000 > Subject: FW: [Histonet] what book to study for registry? > > Robert, > Apart from what is recommended for the registry exam I would > strongly recommend he reads the latest edition of John Kiernan's text, > Histological and Histochemical Methods. Being available in paperback form it > will not make a large dent in his wallet. This text is packed with > information on technique and the background to it, plus, it's well written > and easily read. > Ian. > > Dr. Ian Montgomery, > Histotechnology, > I.B.L.S. Support Unit, > Thomson Building, > University of Glasgow, > Glasgow, > G12 8QQ. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert > Richmond > Sent: 04 December 2009 04:22 > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] what book to study for registry? > > I'm working with a thirtysomething man with a degree in chemistry and > a good bit of experience under his belt, who in these bad times wound > up signing on as a histech with no experience. Trained by an elderly > minimally competent tech, he's rapidly blossomed - is making superb > slides and is really interested in histotechnology. He's appalled by > the minimal knowledge of science of his co-workers. > > With the base ulterior motive of trying to keep him from taking off > when times get better, I've been strongly encouraging him to take the > registry exam when he becomes eligible in a few more months. I'll try > to work with him as much as I can. > > My question to you all is - what book should he be studying? Is the > exam still based on Freida Carson's book (2nd ed) which we have, or is > there a more recent book we should get? > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Chat with Messenger straight from your Hotmail inbox. http://www.microsoft.com/windows/windowslive/hotmail_bl1/hotmail_bl1.aspx?ocid=PID23879::T:WLMTAGL:ON:WL:en-ww:WM_IMHM_4:092009 From dreynold <@t> mdanderson.org Fri Dec 4 08:48:15 2009 From: dreynold <@t> mdanderson.org (Reynolds,Donna M) Date: Fri Dec 4 08:48:25 2009 Subject: [Histonet] RE: IF colocalization with 2 rabbit antibodies In-Reply-To: References: Message-ID: <785BBF0C5F49CE41BA74460A43A08F021641F978BC@DCPWVMBXC0VS3.mdanderson.edu> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, December 03, 2009 11:43 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 73, Issue 5 We have been doing IF Colocaliztion with 2 rabbit antibodies since '04. Our investigators like the labeling. We do one antibody through secondary. After secondary we wash incubate with Rabbit serum 20ug/ml for 30 minutes, wash incubate with unconjugated goat F(ab)2 fragment anti-rabbit F(ab)2 fragment dilute 1:10 incubate 1-2 hr room temp. Wash then add our second primary antibody and proceed as usual. It can make a difference which primary antibody goes first. We always try each antibody alone to see the pattern then do 2 slides rotating which antibody is first and second. Usually both ways will work but often one of the two ways is much cleaner, crisper and stronger. Good luck. Donna Reynolds, Chief Histology Tech, HT (ASCP) core IHC research lab U.T. M.D. Anderson Cancer Center Houston, Texas 713-792-8106 From aevans <@t> wellspan.org Fri Dec 4 08:54:53 2009 From: aevans <@t> wellspan.org (Evans, Andria B) Date: Fri Dec 4 08:55:00 2009 Subject: [Histonet] Cerner Millennium Scroll Capabilities? Message-ID: Does anyone out there have the capability to scroll with your mouse wheel in cerner millennium? Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From kc <@t> ka-recruiting.com Fri Dec 4 08:55:48 2009 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Fri Dec 4 08:55:44 2009 Subject: [Histonet] Histology Supervisor Job Message-ID: <957600390.1259938548502.JavaMail.cfservice@webserver54> Dear Histonet Subscribers, I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have clients across the country. We are completely free of charge to candidates and are currently working on numerous Histology positions. Our clients often assist with relocation expenses. One position in particular I want to tell you about is a 1st shift Histology Supervisor job with one of the top ranked teaching hospitals in the country. Located in New York City, this hospital is an award winning facility that's consistently ranked in the top 10% in the annual "Best Hospital" issue of US News & World Report. Requirements for the position include: BS in Medical Technology (or related science), HT or HTL ASCP certification and NY State License. Must have at least 5 years of experience working as a Histotechnologist in a clinical lab setting with recent supervisory experience. This position offers a competitive compensation package with outstanding benefits including retirement package. Below is a list of some of the other Histology opportunities we are currently working on. 1) New York City - Histotechnologist -3rd shift 2) Oklahoma - Histotechnologist - 1st shift 3) Georgia (SE part of the state) - Histology Supervisor - 1st shift 4) Georgia (SE part of the state) - Histotechnologist - 2 positions open 5) California (Bay Area) - Pathology Manager - 1st shift 6) California (Los Angeles) - Histology Supervisor - 1st shift 7) California (san Diego) - Sr. Histotechnologist - 1st shift 8) Massachusetts (Boston) - Histotechnologist - 1st shift Contact me if you're interested in learning more about any of these opportunities or if you'd like to be considered for future positions that come up with our clients. We work on positions at all levels and cover the entire US. Please also send me an updated resume and let me know what the best way to reach you is. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From WBENTON <@t> umm.edu Fri Dec 4 09:12:50 2009 From: WBENTON <@t> umm.edu (Walter Benton) Date: Fri Dec 4 09:13:11 2009 Subject: [Histonet] Formaldehyde Vapors In-Reply-To: <34CD81B4.506@GWIA2.umm.edu> References: <34CD81B4.506@GWIA2.umm.edu> Message-ID: <4B18E09F.D886.00F4.3@umm.edu> Are you using the potassium permanganate filters? Are you evaluating and changing them as needed. Instructions to evaluate the pellets are listed on page 120 of the Mopec catalogue and probably can be found online. Basically the test has you check the pellets for color, Purple = New, Dark Brown the pellet is spent. We have used these filters for a while now and find that they work well provided they are changed at appropriate intervals. From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Thursday, December 03, 2009 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FORMALDEHYDE Good Morning Histo Land, I was just informed that on my last test sample for formaldehyde vapors was very high. Right now I have to wear a respirator while grossing and assisting grossing. We are looking at why the Mopec grossing station is not working in fact we have not had really good results since we put it in. I'm wondering if anyone else is having the same problem or know of a better solution. Our grossing station is the one that is self contained with filters. Also is anyone using with good result a formalin substitute? If so which one? Thanks and Have a great day, Kathy Gorham, H.T. Grande Ronde Hospital La Grande Oregon Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From cbrya <@t> lexclin.com Fri Dec 4 09:33:24 2009 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Fri Dec 4 09:33:29 2009 Subject: [Histonet] what book to study for registry? In-Reply-To: References: Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF79B4F145@EXCHANGESB> Please reply to all. I am also interested in any suggestions as several histotechs I work with would also benefit from the information. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, December 03, 2009 11:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] what book to study for registry? I'm working with a thirtysomething man with a degree in chemistry and a good bit of experience under his belt, who in these bad times wound up signing on as a histech with no experience. Trained by an elderly minimally competent tech, he's rapidly blossomed - is making superb slides and is really interested in histotechnology. He's appalled by the minimal knowledge of science of his co-workers. With the base ulterior motive of trying to keep him from taking off when times get better, I've been strongly encouraging him to take the registry exam when he becomes eligible in a few more months. I'll try to work with him as much as I can. My question to you all is - what book should he be studying? Is the exam still based on Freida Carson's book (2nd ed) which we have, or is there a more recent book we should get? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From RCazares <@t> schosp.org Fri Dec 4 10:11:43 2009 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Fri Dec 4 10:11:52 2009 Subject: [SPAM-HC] - [Histonet] what book to study for registry? - Email found in subject In-Reply-To: References: Message-ID: <572D1F45B44B3D4096D554B4CB40639C01F27B7493@EXCHCCRMB.schosp.org> I recently mentored an employee who took and passed the certification test. We did use Freida's book but used questions from ASCP's study guide book to supplement. I felt that since ASCP is the entity putting together the test questions, the employee would benefit from being familiar with the type of questions ASCP would use. It's a nice book, divided into the different chapters (fixation, microtomy, processing etc.) and it is also divided into the questions for HT and HTL. In our situation, it helped identify the areas were the student was lacking so that we could focus on that area. Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, December 03, 2009 10:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - [Histonet] what book to study for registry? - Email found in subject I'm working with a thirtysomething man with a degree in chemistry and a good bit of experience under his belt, who in these bad times wound up signing on as a histech with no experience. Trained by an elderly minimally competent tech, he's rapidly blossomed - is making superb slides and is really interested in histotechnology. He's appalled by the minimal knowledge of science of his co-workers. With the base ulterior motive of trying to keep him from taking off when times get better, I've been strongly encouraging him to take the registry exam when he becomes eligible in a few more months. I'll try to work with him as much as I can. My question to you all is - what book should he be studying? Is the exam still based on Freida Carson's book (2nd ed) which we have, or is there a more recent book we should get? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet thwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From cmiller <@t> physlab.com Fri Dec 4 10:40:25 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Dec 4 10:40:32 2009 Subject: [Histonet] what book to study for registry? In-Reply-To: References: Message-ID: ........Am I the only one offended by this ?? I'm working with a thirtysomething man with a degree in chemistry and a good bit of experience under his belt, who in these bad times wound up signing on as a histech with no experience. Trained by an elderly minimally competent tech, he's rapidly blossomed - is making superb slides and is really interested in histotechnology. He's appalled by the minimal knowledge of science of his co-workers. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731- PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From cmiller <@t> physlab.com Fri Dec 4 10:42:13 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Dec 4 10:42:17 2009 Subject: [Histonet] mistake Message-ID: Sorry didn't mean to resend, guess it's a Friday thing?? Maybe not. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From cmiller <@t> physlab.com Fri Dec 4 10:45:33 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Dec 4 10:45:38 2009 Subject: [Histonet] one more time. This is what I meant to send Message-ID: Am I the only one offended by this ?? ....Thank goodness for his co-workers, He will be able to rescue them from their plight....Remember when histology was in a dark dank room next to the morgue?? Show some respect for those elderly minimally competent techs ... Most of them taught themselves histology. (In these bad times wound up signing on as a histech)...Its not as if being a HT or HTL is the worse thing this guy could have stumbled upon. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From cbrya <@t> lexclin.com Fri Dec 4 10:45:35 2009 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Fri Dec 4 10:45:41 2009 Subject: [Histonet] tissue processor times Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF79B4F14A@EXCHANGESB> Hello everyone! We have always had one program (catch-all) for all our specimens that we ran overnight on an Excelsior tissue processor. We just purchased a new VIP 6 and I want to have a program for our larger fatty specimens such as breast cases and run our small biopsies on another program. How much time do you use on each station for your larger cases such as breasts? Particularly the formalin stations, 1 hour or 2 hours each? Thanks in advance for your replies. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From KMB01 <@t> grh.org Fri Dec 4 10:55:15 2009 From: KMB01 <@t> grh.org (Kathy M. Gorham) Date: Fri Dec 4 10:55:23 2009 Subject: [Histonet] THANK YOU Message-ID: It's Friday! Thanks to everyone that responded to my question about formaldehyde fumes. It was helpful. Yes, we do change the filters . . . Monthly. Maintenance came in and at this moment are checking the air flow. It looks like they are going to go ahead and vent it to the hospital system. Until we are tested again we will be wearing respirators. YUCK!! But I want to be safe. Thanks again and have a great week end. Kathy Gorham H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS GRH Mission We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. From cmiller <@t> physlab.com Fri Dec 4 11:05:09 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Dec 4 11:05:18 2009 Subject: [Histonet] RE: one more time. This is what I meant to send In-Reply-To: References: Message-ID: Once more and I will put it to bed. No I am not an old tech... I have been in histology for 18 years. May I quote Dr. Mohs " Only a histotech can produce something so beautiful and useful as a slide" .......sliding off my soapbox . Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From pruegg <@t> ihctech.net Fri Dec 4 12:10:03 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Dec 4 12:11:06 2009 Subject: [Histonet] staining for lipofucsin Message-ID: Does anyone have recommendations for what special stains to use for ffpe rat tissue when we are looking for lipofucsin? Some of the special stains we have investigated include Schmorl's, SBB, Toludine Blue, but the investigator is not really satisfied and expects better results. If anyone has experience with trying to stain lipofucsin in tissue please let me hear from you. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From Nancy.Herman <@t> inspection.gc.ca Fri Dec 4 12:37:56 2009 From: Nancy.Herman <@t> inspection.gc.ca (Nancy Herman) Date: Fri Dec 4 12:38:10 2009 Subject: [Histonet] Clusterin-A (M-18) IHC Message-ID: <20091204T113756Z_6DEB00150000@inspection.gc.ca> Does anyone have a working dilution, retrieval information, etc. for the clusterin-a M-18 antibody (goat polyclonal) from Santa Cruz Biotech? I have already gathered all the information I could from the company. One of our collaborators has given us the antibody to do IHC on mice tissue. Previously, they have only used it for western blots. Thanks for any information Nancy Herman CFIA Lethbridge Laboratory, Lethbridge, Alberta From WBENTON <@t> umm.edu Fri Dec 4 12:39:50 2009 From: WBENTON <@t> umm.edu (Walter Benton) Date: Fri Dec 4 12:40:10 2009 Subject: [Histonet] Cerner In-Reply-To: <739091B4.002@GWIA1.umm.edu> References: <739091B4.002@GWIA1.umm.edu> Message-ID: <4B191124.D886.00F4.3@umm.edu> We are able to. Is there a particular application that you are not able to do this? Message: 1 Date: Fri, 4 Dec 2009 09:54:53 -0500 From: "Evans, Andria B" Subject: [Histonet] Cerner Millennium Scroll Capabilities? To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Does anyone out there have the capability to scroll with your mouse wheel in cerner millennium? Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From jkiernan <@t> uwo.ca Fri Dec 4 12:46:38 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Dec 4 12:46:44 2009 Subject: [Histonet] staining for lipofucsin Message-ID: How about Lillie's Nile blue A method? 0.05% dye in 1% sulphuric acid, 20 mins; wash in running water; aqueous mounting medium. See J. Histochem. Cytochem. 4: 377-381 (1956). Anyone can download this paper for free at www.jhc.org. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Patsy Ruegg Date: Friday, December 4, 2009 13:12 Subject: [Histonet] staining for lipofucsin To: histonet@lists.utsouthwestern.edu > Does anyone have recommendations for what special stains to use > for ffpe rat > tissue when we are looking for lipofucsin? Some of the > special stains we > have investigated include Schmorl's, SBB, Toludine Blue, but the > investigator is not really satisfied and expects better > results. If anyone > has experience with trying to stain lipofucsin in tissue please > let me hear > from you. > > > > Best regards, > > > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of > the Person(s) > ('the intended recipient') to whom it was addressed. Any views > or opinions > presented are solely those of the author. It may contain > information that is > privileged & confidential within the meaning of applicable law. > Accordinglyany dissemination, distribution, copying, or other > use of this message, or > any of its contents, by any person other than the intended > recipient may > constitute a breach of civil or criminal law and is strictly > prohibited. If > you are NOT the intended recipient please contact the sender and > dispose of > this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Fri Dec 4 12:54:24 2009 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Dec 4 12:54:35 2009 Subject: [Histonet] Cerner In-Reply-To: <4B191124.D886.00F4.3@umm.edu> References: <739091B4.002@GWIA1.umm.edu> <4B191124.D886.00F4.3@umm.edu> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A5488@EXCHMBC2.ad.ah.local> It depends upon the version you are on and the application. Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Friday, December 04, 2009 12:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cerner We are able to. Is there a particular application that you are not able to do this? Message: 1 Date: Fri, 4 Dec 2009 09:54:53 -0500 From: "Evans, Andria B" Subject: [Histonet] Cerner Millennium Scroll Capabilities? To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Does anyone out there have the capability to scroll with your mouse wheel in cerner millennium? Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. 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From garret.t.miyamoto <@t> us.army.mil Fri Dec 4 13:47:24 2009 From: garret.t.miyamoto <@t> us.army.mil (Miyamoto, Garret T Mr CIV USA USAMEDCOM) Date: Fri Dec 4 13:47:32 2009 Subject: [Histonet] Re: Tissue processor times In-Reply-To: <0KU500LLN3JSAY80@mail23.us.army.mil> References: <0KU500LLN3JSAY80@mail23.us.army.mil> Message-ID: Carol, We just obtained a VIP 6 processor and we have a program for routine overnight processing. It seems to work well. I will send or fax it to you by Monday. The Tissue-tek sales rep that installed and trained us, mentioned about increasing the times in the alcohols for fatty/breast tissue. You can try this and make any adjustments to the program I am sending. We have two stations of formalin at two hours each. As a side note, we have two Excelsior processors also and except for a few glitches, they seem to work fine. Take care. Garret Miyamoto Lead histotech, Anatomic Pathology Dept. Tripler Army Medical Center ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Friday, December 4, 2009 8:04 am Subject: Histonet Digest, Vol 73, Issue 8 To: histonet@lists.utsouthwestern.edu > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Cerner Millennium Scroll Capabilities? (Evans, Andria B) > 2. Histology Supervisor Job (K.C. Carpenter) > 3. Formaldehyde Vapors (Walter Benton) > 4. RE: what book to study for registry? (Carol Bryant) > 5. RE: [SPAM-HC] - [Histonet] what book to study for registry? - > Email found in subject (Cazares, Ruth) > 6. RE: what book to study for registry? (Cheri Miller) > 7. mistake (Cheri Miller) > 8. one more time. This is what I meant to send (Cheri Miller) > 9. tissue processor times (Carol Bryant) > 10. THANK YOU (Kathy M. Gorham) > 11. RE: one more time. This is what I meant to send (Cheri Miller) > > > ------------------------------------------------------------------- > --- > > Message: 1 > Date: Fri, 4 Dec 2009 09:54:53 -0500 > From: "Evans, Andria B" < > Subject: [Histonet] Cerner Millennium Scroll Capabilities? > To: < > Message-ID: > < > Content-Type: text/plain; charset="iso-8859-1" > > Does anyone out there have the capability to scroll with your mouse wheel in cerner millennium? > > Andria B Evans, HTL(ASCP)CM > Anatomic Pathology > York Hospital > 1001 S. George Street > York, PA 17405 > 717-851-5006 > > "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe > > "Maturity is accepting imperfections." > > CONFIDENTIALITY NOTICE: > > This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . > > > ______________________________________________________________________ > This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. > ______________________________________________________________________ > > > > ------------------------------ > > Message: 2 > Date: 4 Dec 2009 09:55:48 -0500 > From: "K.C. Carpenter" < > Subject: [Histonet] Histology Supervisor Job > To: histonet@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset="utf-8" > > > > > Dear Histonet Subscribers, > > > > I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have clients across the country. We are completely free of charge to candidates and are currently working on numerous Histology positions. Our clients often assist with relocation expenses. > > > > > > > > One position in particular I want to tell you about is a 1st shift Histology Supervisor job with one of the top ranked teaching hospitals in the country. Located in New York City, this hospital is an award winning facility that's consistently ranked in the top 10% in the annual "Best Hospital" issue of US News & World Report. Requirements for the position include: BS in Medical Technology (or related science), HT or HTL ASCP certification and NY State License. Must have at least 5 years of experience working as a Histotechnologist in a clinical lab setting with recent supervisory experience. This position offers a competitive compensation package with outstanding benefits including retirement package. > > > > Below is a list of some of the other Histology opportunities we are currently working on. > > > > 1) New York City - Histotechnologist -3rd shift > > 2) Oklahoma - Histotechnologist - 1st shift > > 3) Georgia (SE part of the state) - Histology Supervisor - 1st shift > > 4) Georgia (SE part of the state) - Histotechnologist - 2 positions open > > 5) California (Bay Area) - Pathology Manager - 1st shift > > 6) California (Los Angeles) - Histology Supervisor - 1st shift > > 7) California (san Diego) - Sr. Histotechnologist - 1st shift > > 8) Massachusetts (Boston) - Histotechnologist - 1st shift > > > > > > > > > > > > > > > > > > > > > > > > Contact me if you're interested in learning more about any of these opportunities or if you'd like to be considered for future positions that come up with our clients. We work on positions at all levels and cover the entire US. Please also send me an updated resume and let me know what the best way to reach you is. > > > > > To view some additional opportunities please visit our website at www.ka-recruiting.com. > > > > > > > > > > > > > > > > > Sincerely > > > > > > > > > > > > > > KC Carpenter > > > > K.A. Recruiting, Inc. > > > 10 Post Office Square, 8th Floor South > > > Boston, MA 02109 > > > P: (617) 692-2949 > > > F: (617) 507-8009 > > > > kc@ka-recruiting.com > > > > www.ka-recruiting.com > > > > > > > > > > > > ------------------------------ > > Message: 3 > Date: Fri, 04 Dec 2009 10:12:50 -0500 > From: "Walter Benton" < > Subject: [Histonet] Formaldehyde Vapors > To: < > Message-ID: < > Content-Type: text/plain; charset=US-ASCII > > Are you using the potassium permanganate filters? Are you evaluating and changing them as needed. Instructions to evaluate the pellets are listed on page 120 of the Mopec catalogue and probably can be found online. Basically the test has you check the pellets for color, Purple = New, Dark Brown the pellet is spent. We have used these filters for a while now and find that they work well provided they are changed at appropriate intervals. > > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy M. > Gorham > Sent: Thursday, December 03, 2009 11:36 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] FORMALDEHYDE > > Good Morning Histo Land, I was just informed that on my last test sample > for formaldehyde vapors was very high. Right now I have to wear a > respirator while grossing and assisting grossing. We are looking at why > the Mopec grossing station is not working in fact we have not had really > good results since we put it in. I'm wondering if anyone else is having > the same problem or know of a better solution. Our grossing station is > the one that is self contained with filters. Also is anyone using with > good result a formalin substitute? If so which one? > Thanks and Have a great day, > > Kathy Gorham, H.T. > Grande Ronde Hospital > La Grande Oregon > > > Walter Benton, HT(ASCP)QIHC > Histology Supervisor > University of Maryland Medical Center > Anatomic Pathology > 22 S. Greene St > Room NBW65 > Baltimore MD 21201 > (Direct) 410-328-0930 > (Lab) 410-328-5524 > (Fax) 410-328-5508 > > > > This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. > > > > ------------------------------ > > Message: 4 > Date: Fri, 4 Dec 2009 10:33:24 -0500 > From: Carol Bryant < > Subject: RE: [Histonet] what book to study for registry? > To: 'Robert Richmond' <, > "Histonet@lists.utsouthwestern.edu" > < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Please reply to all. I am also interested in any suggestions as several histotechs I work with would also benefit from the information. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Thursday, December 03, 2009 11:22 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] what book to study for registry? > > I'm working with a thirtysomething man with a degree in chemistry and > a good bit of experience under his belt, who in these bad times wound > up signing on as a histech with no experience. Trained by an elderly > minimally competent tech, he's rapidly blossomed - is making superb > slides and is really interested in histotechnology. He's appalled by > the minimal knowledge of science of his co-workers. > > With the base ulterior motive of trying to keep him from taking off > when times get better, I've been strongly encouraging him to take the > registry exam when he becomes eligible in a few more months. I'll try > to work with him as much as I can. > > My question to you all is - what book should he be studying? Is the > exam still based on Freida Carson's book (2nd ed) which we have, or is > there a more recent book we should get? > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > NOTICE OF CONFIDENTIALITY > > This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. > > > > > ------------------------------ > > Message: 5 > Date: Fri, 4 Dec 2009 10:11:43 -0600 > From: "Cazares, Ruth" < > Subject: RE: [SPAM-HC] - [Histonet] what book to study for registry? - > Email found in subject > To: Robert Richmond <, > "Histonet@lists.utsouthwestern.edu" > < > Message-ID: > < > Content-Type: text/plain; charset="us-ascii" > > I recently mentored an employee who took and passed the certification test. We did use Freida's book but used questions from ASCP's study guide book to supplement. I felt that since ASCP is the entity putting together the test questions, the employee would benefit from being familiar with the type of questions ASCP would use. It's a nice book, divided into the different chapters (fixation, microtomy, processing etc.) and it is also divided into the questions for HT and HTL. > > In our situation, it helped identify the areas were the student was lacking so that we could focus on that area. > > > Ruth Cazares, HT (ASCP) > Histology Supervisor > Department of Pathology > Swedish Covenant Hospital > 5145 North California Ave > Chicago, IL 60625 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond > Sent: Thursday, December 03, 2009 10:22 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [SPAM-HC] - [Histonet] what book to study for registry? - Email found in subject > > I'm working with a thirtysomething man with a degree in chemistry and a good bit of experience under his belt, who in these bad times wound up signing on as a histech with no experience. Trained by an elderly minimally competent tech, he's rapidly blossomed - is making superb slides and is really interested in histotechnology. He's appalled by the minimal knowledge of science of his co-workers. > > With the base ulterior motive of trying to keep him from taking off when times get better, I've been strongly encouraging him to take the registry exam when he becomes eligible in a few more months. I'll try to work with him as much as I can. > > My question to you all is - what book should he be studying? Is the exam still based on Freida Carson's book (2nd ed) which we have, or is there a more recent book we should get? > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > thwestern.edu/mailman/listinfo/histonet > > *** Confidentiality Statement *** > This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. > > > Thank you for your cooperation. > > > > ------------------------------ > > Message: 6 > Date: Fri, 4 Dec 2009 10:40:25 -0600 > From: Cheri Miller < > Subject: RE: [Histonet] what book to study for registry? > To: "Histonet@lists.utsouthwestern.edu" > < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > ........Am I the only one offended by this ?? > I'm working with a thirtysomething man with a degree in chemistry and a good bit of experience under his belt, who in these bad times wound up signing on as a histech with no experience. Trained by an elderly minimally competent tech, he's rapidly blossomed - is making superb slides and is really interested in histotechnology. He's appalled by the minimal knowledge of science of his co-workers. > > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731- > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > > > > ------------------------------ > > Message: 7 > Date: Fri, 4 Dec 2009 10:42:13 -0600 > From: Cheri Miller < > Subject: [Histonet] mistake > To: "Histonet@lists.utsouthwestern.edu" > < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Sorry didn't mean to resend, guess it's a Friday thing?? Maybe not. > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > > > > ------------------------------ > > Message: 8 > Date: Fri, 4 Dec 2009 10:45:33 -0600 > From: Cheri Miller < > Subject: [Histonet] one more time. This is what I meant to send > To: "Histonet@lists.utsouthwestern.edu" > < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Am I the only one offended by this ?? ....Thank goodness for his co-workers, He will be able to rescue them from their plight....Remember when histology was in a dark dank room next to the morgue?? Show some respect for those elderly minimally competent techs ... Most of them taught themselves histology. (In these bad times wound up signing on as a histech)...Its not as if being a HT or HTL is the worse thing this guy could have stumbled upon. > > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > > > > ------------------------------ > > Message: 9 > Date: Fri, 4 Dec 2009 11:45:35 -0500 > From: Carol Bryant < > Subject: [Histonet] tissue processor times > To: "Histonet@lists.utsouthwestern.edu" > < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Hello everyone! > We have always had one program (catch-all) for all our specimens that we ran overnight on an Excelsior tissue processor. We just purchased a new VIP 6 and I want to have a program for our larger fatty specimens such as breast cases and run our small biopsies on another program. How much time do you use on each station for your larger cases such as breasts? Particularly the formalin stations, 1 hour or 2 hours each? > Thanks in advance for your replies. > > > Carol Bryant, CT (ASCP) > Cytology/Histology Manager > Pathology Services > Lexington Clinic > Phone (859) 258-4082 > Fax (859) 258-4081 > cbrya@lexclin.com > > > > NOTICE OF CONFIDENTIALITY > > This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. > > > ------------------------------ > > Message: 10 > Date: Fri, 4 Dec 2009 08:55:15 -0800 > From: "Kathy M. Gorham" < > Subject: [Histonet] THANK YOU > To: < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > It's Friday! Thanks to everyone that responded to my question about > formaldehyde fumes. It was helpful. Yes, we do change the filters . . . > Monthly. Maintenance came in and at this moment are checking the air > flow. It looks like they are going to go ahead and vent it to the > hospital system. Until we are tested again we will be wearing > respirators. YUCK!! But I want to be safe. Thanks again and have a > great week end. > > Kathy Gorham H.T. > > > GRH National Recognition > Outstanding Rural Health Organization of 2009 awarded by NRHA > Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP > Leader in Innovative Excellence 2009 awarded by the OAHHS > > GRH Mission > We will ensure ACCESS to superior QUALITY, affordable health care for all those in need of our services, including underserved populations within our communities. > > > GRH Confidentiality Notice > This e-mail and any attached documents are for the intended recipient/s only > and should be protected against viewing by unauthorized persons. The information > herein may have been disclosed from records whose confidentiality is protected > by Federal and State Law. Federal regulations prohibit further distribution or > copying of this information without permission. If you received this e-mail > transmission in error, please notify the sender immediately to arrange for return > or destruction of this information. > > > > ------------------------------ > > Message: 11 > Date: Fri, 4 Dec 2009 11:05:09 -0600 > From: Cheri Miller < > Subject: [Histonet] RE: one more time. This is what I meant to send > To: "Histonet@lists.utsouthwestern.edu" > < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > > Once more and I will put it to bed. No I am not an old tech... I have been in histology for 18 years. May I quote Dr. Mohs " Only a histotech can produce something so beautiful and useful as a slide" .......sliding off my soapbox . > > Cheryl Miller HT ASCP CM > Histology Supervisor > Physicians Laboratory Services > Omaha, NE. 402 731 4148 > > -----Original Message----- > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 73, Issue 8 > *************************************** From liz <@t> premierlab.com Fri Dec 4 14:14:16 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Dec 4 14:14:20 2009 Subject: [Histonet] Clusterin-A (M-18) IHC In-Reply-To: <20091204T113756Z_6DEB00150000@inspection.gc.ca> Message-ID: Nancy We have used the clusterin alpha from santa cruz on mouse tissue sections, the one we have used is a rabbit polyclonal sc-8354, we have not worked with the M-18 antibody. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Herman Sent: Friday, December 04, 2009 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Clusterin-A (M-18) IHC Does anyone have a working dilution, retrieval information, etc. for the clusterin-a M-18 antibody (goat polyclonal) from Santa Cruz Biotech? I have already gathered all the information I could from the company. One of our collaborators has given us the antibody to do IHC on mice tissue. Previously, they have only used it for western blots. Thanks for any information Nancy Herman CFIA Lethbridge Laboratory, Lethbridge, Alberta From plucas <@t> biopath.org Fri Dec 4 14:31:49 2009 From: plucas <@t> biopath.org (Paula Lucas) Date: Fri Dec 4 14:31:49 2009 Subject: [Histonet] Digital Flotation Bath Message-ID: <000001ca7520$ced50270$0f01a8c0@biopath.local> Hello, What is the best digital flotation bath out on the market that will keep/maintain its temperature when it's in use? Thanks in advance, Paula Bio-Path Medical Group From HornHV <@t> archildrens.org Fri Dec 4 17:08:12 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Dec 4 17:08:28 2009 Subject: [Histonet] what book to study for registry? In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D835C7@EMAIL.archildrens.org> "Trained by an elderly minimally competent tech, he's rapidly blossomed - is making superb slides and is really interested in histotechnology. He's appalled by the minimal knowledge of science of his co-workers." Gee I wonder how he is able to make superb slides being trained by an elderly minimally competent tech?? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Friday, December 04, 2009 2:16 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] what book to study for registry? Robert, Apart from what is recommended for the registry exam I would strongly recommend he reads the latest edition of John Kiernan's text, Histological and Histochemical Methods. Being available in paperback form it will not make a large dent in his wallet. This text is packed with information on technique and the background to it, plus, it's well written and easily read. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: 04 December 2009 04:22 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] what book to study for registry? I'm working with a thirtysomething man with a degree in chemistry and a good bit of experience under his belt, who in these bad times wound up signing on as a histech with no experience. Trained by an elderly minimally competent tech, he's rapidly blossomed - is making superb slides and is really interested in histotechnology. He's appalled by the minimal knowledge of science of his co-workers. With the base ulterior motive of trying to keep him from taking off when times get better, I've been strongly encouraging him to take the registry exam when he becomes eligible in a few more months. I'll try to work with him as much as I can. My question to you all is - what book should he be studying? Is the exam still based on Freida Carson's book (2nd ed) which we have, or is there a more recent book we should get? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From lpwenk <@t> sbcglobal.net Fri Dec 4 18:46:51 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Dec 4 18:47:02 2009 Subject: [Histonet] staining for lipofucsin In-Reply-To: Message-ID: <65EAFB8504C14691ADA610AE69E60C37@HPPav2> Fontana-Masson, for argentaffin/melanin. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, December 04, 2009 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staining for lipofucsin Does anyone have recommendations for what special stains to use for ffpe rat tissue when we are looking for lipofucsin? Some of the special stains we have investigated include Schmorl's, SBB, Toludine Blue, but the investigator is not really satisfied and expects better results. If anyone has experience with trying to stain lipofucsin in tissue please let me hear from you. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Sat Dec 5 10:33:58 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sat Dec 5 10:34:05 2009 Subject: [Histonet] Joe-animal vs human (and research vs. clinical) Message-ID: <5024.89983.qm@web50907.mail.re2.yahoo.com> ?Hi Joe-- ? I've done both kinds of histo including research animal and veterinary dx as well as people parts,?and I've hired many?techs transitioning both ways. What you've read it pretty much so...with consideration for pace. ? Going from animal to human is EASY!? Going the other way is a little harder.? Human tissue labs have optimal processing, consistent densities, rarely over dried or hard to cut (yes, there are exceptions but have you ever cut over-processed rat spleen, baluga whale blubber or seal skin with 600 hairs/sq cm??? EEEK!!) ? What you will want to be aware of is the PACE of the work.? If he's coming from research, he may need a little prompting for keeping his speed up.? In my experience research has a more varied pace of work.? Clinical labs are full speed 95% of the time. ? A little support with knowing his tissue types and pacing his production?and your new tech?should be just fine. ? Cheryl Kerry,HT(ASCP) Full Staff Inc. 800.756.3309 From carl.hobbs <@t> kcl.ac.uk Sat Dec 5 12:53:37 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Dec 5 12:54:24 2009 Subject: [Histonet] Re: staining for lipofucsin Message-ID: <11D9615B89C10747B1C985966A63D7CA2C3F25A322@KCL-MAIL04.kclad.ds.kcl.ac.uk> Or is it "lipofuscin"? In my Xp there is no specific marker. The fact that your Pathologist is questioning your results, Patsy, amazes me, given your wide experience. Your advice has informed my Practice on many occassions. NB:I still reverentially and enjoyably open my Ham's Histology ( 6th edition) to be entertained and informed:" lipochrome" comes to my mind. Sure, lipofuscin detection must have moved on ? Respectfully, carl From njblademaster <@t> gmail.com Sat Dec 5 13:46:09 2009 From: njblademaster <@t> gmail.com (Nathan Jentsch) Date: Sat Dec 5 13:46:37 2009 Subject: [Histonet] Bachelor's Degrees Message-ID: <5a7745af0912051146m18fb7211vd630af69ee975654@mail.gmail.com> Paula, Let me tell you that this is an extremely frustrating point for me not for getting a job but for getting a license in New York State (which is related because I'm technically supposed to have a license to work). Despite the fact that I have a B.S. in a science field and have been working competently at my job for almost two years now, the state wants me to have an A.S. in histotechnology to get my license. They won't even consider HT certification as sufficient. If a collective group of experts in the fields of laboratory science and pathology say I'm qualified, why isn't that good enough for a bunch of beurocrats who can't even manage the pocket book of our state. Nate From tjasper <@t> copc.net Sat Dec 5 14:23:29 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Sat Dec 5 14:23:36 2009 Subject: [Histonet] Bachelor's Degrees References: <5a7745af0912051146m18fb7211vd630af69ee975654@mail.gmail.com> Message-ID: <90354A475B420441B2A0396E5008D4965E30B0@copc-sbs.COPC.local> One more reason to consider "carefully" before throwing support to state licensure where it does not exist. I feel sorry for you Nathan and I'd like to have someone explain the upside of licensing to you. It seems it's not about having a license (like a driver's license) to practice histology. I fear it's just more about fattening state coffers and adding another level of bureauracracy to things. If you are educated (as you are Nate) and if you are academically eligible to sit for the registry exam. And if you can satisfactorily pass the exam, what has state licensing got to do with it? Are you a better histologist in New York because you're "licensed" as opposed to your neighbors in PA, for example who aren't? I think not. Does licensing prove something that science degrees and registry certifications do not? Maybe I just don't get it. And I'm not trying to pick a fight here with the supporters of licensing. I just haven't heard a good convincing argument for it yet. I'm also quite certain that even though monetary compensation has improved somewhat, the last thing most Histologists need is another payment. The privilege to work in a certain state, which is paid for (by you) nothing more?! I suppose some kindly employers out there somewhere could pay for it...good luck with that. Here's an idea, let's say you're degreed and registry eligible and/or have passed your board exam(s) and are certified. How 'bout the state says you've met the qualification for licensing, here you go! Nate you are degreed and certified and in my book and in the book of the current state I live in - Oregon - and the states I've worked in - Wisconsin, Michigan and Minnesota - you are more than qualified to work. I for one would not hesitate in the least to consider a person such as yourself for employment. Again you are more than qualified, even though you are "unlicensed". I guess I just don't understand how credentialing - degrees and certifications - aren't enough, but licensing is the magic ticket to better science/medicine/patient care/whatever. I'm sure some folks out there will bring on the firestorm, but again Nathan I feel sorry for you and I don't see the reasoning behind this. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nathan Jentsch Sent: Saturday, December 05, 2009 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bachelor's Degrees Paula, Let me tell you that this is an extremely frustrating point for me not for getting a job but for getting a license in New York State (which is related because I'm technically supposed to have a license to work). Despite the fact that I have a B.S. in a science field and have been working competently at my job for almost two years now, the state wants me to have an A.S. in histotechnology to get my license. They won't even consider HT certification as sufficient. If a collective group of experts in the fields of laboratory science and pathology say I'm qualified, why isn't that good enough for a bunch of beurocrats who can't even manage the pocket book of our state. Nate _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Sat Dec 5 15:16:42 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Sat Dec 5 15:15:26 2009 Subject: [Histonet] Bachelor's Degrees In-Reply-To: <90354A475B420441B2A0396E5008D4965E30B0@copc-sbs.COPC.local> References: <5a7745af0912051146m18fb7211vd630af69ee975654@mail.gmail.com><90354A475B420441B2A0396E5008D4965E30B0@copc-sbs.COPC.local> Message-ID: <1542700356-1260047721-cardhu_decombobulator_blackberry.rim.net-131540213-@bda577.bisx.prod.on.blackberry> It is a capitalist environment. If you are ht through ASCP that should be all that counts. This is a way for states to collect extra funds Roxanne Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Thomas Jasper" Date: Sat, 5 Dec 2009 12:23:29 To: Nathan Jentsch Cc: Subject: RE: [Histonet] Bachelor's Degrees One more reason to consider "carefully" before throwing support to state licensure where it does not exist. I feel sorry for you Nathan and I'd like to have someone explain the upside of licensing to you. It seems it's not about having a license (like a driver's license) to practice histology. I fear it's just more about fattening state coffers and adding another level of bureauracracy to things. If you are educated (as you are Nate) and if you are academically eligible to sit for the registry exam. And if you can satisfactorily pass the exam, what has state licensing got to do with it? Are you a better histologist in New York because you're "licensed" as opposed to your neighbors in PA, for example who aren't? I think not. Does licensing prove something that science degrees and registry certifications do not? Maybe I just don't get it. And I'm not trying to pick a fight here with the supporters of licensing. I just haven't heard a good convincing argument for it yet. I'm also quite certain that even though monetary compensation has improved somewhat, the last thing most Histologists need is another payment. The privilege to work in a certain state, which is paid for (by you) nothing more?! I suppose some kindly employers out there somewhere could pay for it...good luck with that. Here's an idea, let's say you're degreed and registry eligible and/or have passed your board exam(s) and are certified. How 'bout the state says you've met the qualification for licensing, here you go! Nate you are degreed and certified and in my book and in the book of the current state I live in - Oregon - and the states I've worked in - Wisconsin, Michigan and Minnesota - you are more than qualified to work. I for one would not hesitate in the least to consider a person such as yourself for employment. Again you are more than qualified, even though you are "unlicensed". I guess I just don't understand how credentialing - degrees and certifications - aren't enough, but licensing is the magic ticket to better science/medicine/patient care/whatever. I'm sure some folks out there will bring on the firestorm, but again Nathan I feel sorry for you and I don't see the reasoning behind this. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nathan Jentsch Sent: Saturday, December 05, 2009 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bachelor's Degrees Paula, Let me tell you that this is an extremely frustrating point for me not for getting a job but for getting a license in New York State (which is related because I'm technically supposed to have a license to work). Despite the fact that I have a B.S. in a science field and have been working competently at my job for almost two years now, the state wants me to have an A.S. in histotechnology to get my license. They won't even consider HT certification as sufficient. If a collective group of experts in the fields of laboratory science and pathology say I'm qualified, why isn't that good enough for a bunch of beurocrats who can't even manage the pocket book of our state. Nate _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Dec 5 15:32:16 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Dec 5 15:32:22 2009 Subject: [Histonet] Bachelor's Degrees In-Reply-To: <1542700356-1260047721-cardhu_decombobulator_blackberry.rim.net-131540213-@bda577.bisx.prod.on.blackberry> Message-ID: <388404.55076.qm@web65701.mail.ac4.yahoo.com> Even if?it is a totally?frustrating issue, it does not matter what we think or wish should happen. The problem resides in how the state resolution that instituted the NY licensure was redacted. That is the regulation we wanted or not, no matter how unfair or even stupid it may be. The problem does not reside in the states having licensures, but how under what rules?they?are created. Once those rules are adopted, it is almost impossible to change them. So, unfortunately,?you will have to follow their rules, you like it or not. Ren? J. ? ? --- On Sat, 12/5/09, godsgalnow@aol.com wrote: From: godsgalnow@aol.com Subject: Re: [Histonet] Bachelor's Degrees To: "Thomas Jasper" , histonet-bounces@lists.utsouthwestern.edu, "Nathan Jentsch" Cc: histonet@lists.utsouthwestern.edu Date: Saturday, December 5, 2009, 4:16 PM It is a capitalist environment.? If you are ht through ASCP that should be all that counts.? This is a way for states to collect extra funds Roxanne Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Thomas Jasper" Date: Sat, 5 Dec 2009 12:23:29 To: Nathan Jentsch Cc: Subject: RE: [Histonet] Bachelor's Degrees One more reason to consider "carefully" before throwing support to state licensure where it does not exist.? I feel sorry for you Nathan and I'd like to have someone explain the upside of licensing to you.? It seems it's not about having a license (like a driver's license) to practice histology.? I fear it's just more about fattening state coffers and adding another level of bureauracracy to things. If you are educated (as you are Nate) and if you are academically eligible to sit for the registry exam.? And if you can satisfactorily pass the exam, what has state licensing got to do with it?? Are you a better histologist in New York because you're "licensed" as opposed to your neighbors in PA, for example who aren't?? I think not. Does licensing prove something that science degrees and registry certifications do not?? Maybe I just don't get it.? And I'm not trying to pick a fight here with the supporters of licensing.? I just haven't heard a good convincing argument for it yet.? I'm also quite certain that even though monetary compensation has improved somewhat, the last thing most Histologists need is another payment.? The privilege to work in a certain state, which is paid for (by you) nothing more?!? I suppose some kindly employers out there somewhere could pay for it...good luck with that.? Here's an idea, let's say you're degreed and registry eligible and/or have passed your board exam(s) and are certified.? How 'bout the state says you've met the qualification for licensing, here you go!? Nate you are degreed and certified and in my book and in the book of the current state I live in - Oregon - and the states I've worked in - Wisconsin, Michigan and Minnesota - you are more than qualified to work.? I for one would not hesitate in the least to consider a person such as yourself for employment.? Again you are more than qualified, even though you are "unlicensed". I guess I just don't understand how credentialing - degrees and certifications - aren't enough, but licensing is the magic ticket to better science/medicine/patient care/whatever.? I'm sure some folks out there will bring on the firestorm, but again Nathan I feel sorry for you and I don't see the reasoning behind this. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nathan Jentsch Sent: Saturday, December 05, 2009 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bachelor's Degrees Paula, Let me tell you that this is an extremely frustrating point for me not for getting a job but for getting a license in New York State (which is related because I'm technically supposed to have a license to work). Despite the fact that I have a B.S. in a science field and have been working competently at my job for almost two years now, the state wants me to have an A.S. in histotechnology to get my license.? They won't even consider HT certification as sufficient.? If a collective group of experts in the fields of laboratory science and pathology say I'm qualified, why isn't that good enough for a bunch of beurocrats who can't even manage the pocket book of our state. Nate _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat Dec 5 21:20:03 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat Dec 5 21:20:10 2009 Subject: [Histonet] Re: staining for lipofuscin Message-ID: Worthwhile to get the name of the stuff straight - Lipofuscin - pronounced LIE-po-FUSS-in - from the Latin word fuscus, 'dark' - is the yellow-brown pigment. Often confused with fuchsin - FYOOK-sin - dyes named after the color fuscia (FYOO-sha) which is named after the flower, which is named after somebody named Fuchs (FOOKS). Confusing. Bob Richmond Samurai Pathologist Knoxville TN From annigyg <@t> gmail.com Sun Dec 6 02:04:20 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Sun Dec 6 02:04:31 2009 Subject: [Histonet] Bachelor's Degrees In-Reply-To: <388404.55076.qm@web65701.mail.ac4.yahoo.com> References: <1542700356-1260047721-cardhu_decombobulator_blackberry.rim.net-131540213-@bda577.bisx.prod.on.blackberry> <388404.55076.qm@web65701.mail.ac4.yahoo.com> Message-ID: my sympathies here in the UAE, in the emirate of Abu Dhabi health care is governed by HAAD (Health Authority of Abu Dhabi) I have a licence issued in South Africa (accepted here) I am also the only person setting the HAAD licencing exam for Histotechs (in the emirate of AD) for all private labs HAAD now need all techs to be licenced in the emirate (like a state or a province) in which they work and so they are slowly issuing these coveted licences as the only recognised 'authority' who single-handedly sets and marks the HAAD Histology licence exam and passes/fails candidates, one would think that I should be automatically granted a HAAD licence... not so... they will issue my licence determined by the position I hold in the organisation which employs me and because my title has changed (NOT my job discription) they will now re-assess my credentials!! so dont complain guys - I think my confusion is greater than yours!!! AbuDhabiAnnie 2009/12/6 Rene J Buesa > Even if it is a totally frustrating issue, it does not matter what we think > or wish should happen. > The problem resides in how the state resolution that instituted the NY > licensure was redacted. That is the regulation we wanted or not, no matter > how unfair or even stupid it may be. > The problem does not reside in the states having licensures, but how under > what rules they are created. Once those rules are adopted, it is almost > impossible to change them. > So, unfortunately, you will have to follow their rules, you like it or not. > Ren? J. > > > > --- On Sat, 12/5/09, godsgalnow@aol.com wrote: > > > From: godsgalnow@aol.com > Subject: Re: [Histonet] Bachelor's Degrees > To: "Thomas Jasper" , > histonet-bounces@lists.utsouthwestern.edu, "Nathan Jentsch" < > njblademaster@gmail.com> > Cc: histonet@lists.utsouthwestern.edu > Date: Saturday, December 5, 2009, 4:16 PM > > > It is a capitalist environment. If you are ht through ASCP that should be > all that counts. This is a way for states to collect extra funds > > Roxanne > Sent from my Verizon Wireless BlackBerry > > -----Original Message----- > From: "Thomas Jasper" > Date: Sat, 5 Dec 2009 12:23:29 > To: Nathan Jentsch > Cc: > Subject: RE: [Histonet] Bachelor's Degrees > > One more reason to consider "carefully" before throwing support to state > licensure where it does not exist. I feel sorry for you Nathan and I'd > like to have someone explain the upside of licensing to you. It seems > it's not about having a license (like a driver's license) to practice > histology. I fear it's just more about fattening state coffers and > adding another level of bureauracracy to things. > > If you are educated (as you are Nate) and if you are academically > eligible to sit for the registry exam. And if you can satisfactorily > pass the exam, what has state licensing got to do with it? Are you a > better histologist in New York because you're "licensed" as opposed to > your neighbors in PA, for example who aren't? I think not. > > Does licensing prove something that science degrees and registry > certifications do not? Maybe I just don't get it. And I'm not trying > to pick a fight here with the supporters of licensing. I just haven't > heard a good convincing argument for it yet. I'm also quite certain > that even though monetary compensation has improved somewhat, the last > thing most Histologists need is another payment. The privilege to work > in a certain state, which is paid for (by you) nothing more?! > > I suppose some kindly employers out there somewhere could pay for > it...good luck with that. Here's an idea, let's say you're degreed and > registry eligible and/or have passed your board exam(s) and are > certified. How 'bout the state says you've met the qualification for > licensing, here you go! Nate you are degreed and certified and in my > book and in the book of the current state I live in - Oregon - and the > states I've worked in - Wisconsin, Michigan and Minnesota - you are more > than qualified to work. I for one would not hesitate in the least to > consider a person such as yourself for employment. Again you are more > than qualified, even though you are "unlicensed". > > I guess I just don't understand how credentialing - degrees and > certifications - aren't enough, but licensing is the magic ticket to > better science/medicine/patient care/whatever. I'm sure some folks out > there will bring on the firestorm, but again Nathan I feel sorry for you > and I don't see the reasoning behind this. > > Tom Jasper > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services > Bend, Oregon 97701 > 541/693-2677 > tjasper@copc.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nathan > Jentsch > Sent: Saturday, December 05, 2009 11:46 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Bachelor's Degrees > > Paula, > Let me tell you that this is an extremely frustrating point for me not > for getting a job but for getting a license in New York State (which is > related because I'm technically supposed to have a license to work). > Despite the fact that I have a B.S. in a science field and have been > working competently at my job for almost two years now, the state wants > me to have an A.S. in histotechnology to get my license. They won't > even consider HT certification as sufficient. If a collective group of > experts in the fields of laboratory science and pathology say I'm > qualified, why isn't that good enough for a bunch of beurocrats who > can't even manage the pocket book of our state. > > Nate > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -----Inline Attachment Follows----- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From gu.lang <@t> gmx.at Sun Dec 6 02:44:02 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Dec 6 02:44:16 2009 Subject: [Histonet] OT fuchsia In-Reply-To: References: Message-ID: Hi Bob, You made me wondering if your explanation of the flower's name is true, because German Fuchs means fox and I grew up with the believing, that the name is derived from the red colour of the fox. - But I was wrong. I found the history of the exploration on a Fuchsien-website. The discoverer was the Franziskaner-Monk Charles Plumier, born on 20th April 1646 in Marseille. He was sent by Louis XIV to Santa Domingo (Dom.Rep.). There he found the flowerbush and called it "Fuchsia triphylla flore coccinea" after Leonhart Fuchs (1501-1566), a German botanist and medic. Perhaps I tell you nothing new, but for me it was just interesting to look it up. Regards Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Robert Richmond Gesendet: Sonntag, 06. Dezember 2009 04:20 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: staining for lipofuscin Worthwhile to get the name of the stuff straight - Lipofuscin - pronounced LIE-po-FUSS-in - from the Latin word fuscus, 'dark' - is the yellow-brown pigment. Often confused with fuchsin - FYOOK-sin - dyes named after the color fuscia (FYOO-sha) which is named after the flower, which is named after somebody named Fuchs (FOOKS). Confusing. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LilianaB <@t> asaf.health.gov.il Sun Dec 6 06:18:30 2009 From: LilianaB <@t> asaf.health.gov.il (LilianaB@asaf.health.gov.il) Date: Sun Dec 6 06:19:27 2009 Subject: [Histonet] Celerus wave autoimmunostainer Message-ID: <054452CCC076BE4DA21E46AE95E32EC304B451@mail2.asaf.health.gov.il> Hello to everybody Does anyone know the autoimmunostainer from Celerus? It is supposed to perform immunostaining in about 15 minutes. We are interested in the system for testing presence of micrometastases in sentinel lymph nodes (on frozen sections), and for performing urgent immunostaining on paraffin sections Does anybody have experience with this system? Thank you in advance Liliana Habler Department of Pathology Asaf Harofeh Medical Center Israel From pruegg <@t> ihctech.net Sun Dec 6 10:32:19 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Dec 6 10:32:59 2009 Subject: SPAM-LOW: [Histonet] Re: staining for lipofuscin In-Reply-To: References: Message-ID: Thank you for the schooling Samurai, I am ignorant in these matters and do appreciate being more educated. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Saturday, December 05, 2009 8:20 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Re: staining for lipofuscin Worthwhile to get the name of the stuff straight - Lipofuscin - pronounced LIE-po-FUSS-in - from the Latin word fuscus, 'dark' - is the yellow-brown pigment. Often confused with fuchsin - FYOOK-sin - dyes named after the color fuscia (FYOO-sha) which is named after the flower, which is named after somebody named Fuchs (FOOKS). Confusing. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Dec 6 10:40:22 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Dec 6 10:41:01 2009 Subject: SPAM-LOW: [Histonet] OT fuchsia In-Reply-To: References: Message-ID: <0DCA214C9BFF4E15AAED2AAD18F31CE2@prueggihctechlt> I love hearing about the history of Histology, I always ponder how things were discovered, like how the heck did someone figure out that if you stained micorganisms with a dye and then treated them with acid they would stay stained (be acid fast). This motivates me to discover things myself. I love what we do. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Sunday, December 06, 2009 1:44 AM To: 'Robert Richmond' Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] OT fuchsia Hi Bob, You made me wondering if your explanation of the flower's name is true, because German Fuchs means fox and I grew up with the believing, that the name is derived from the red colour of the fox. - But I was wrong. I found the history of the exploration on a Fuchsien-website. The discoverer was the Franziskaner-Monk Charles Plumier, born on 20th April 1646 in Marseille. He was sent by Louis XIV to Santa Domingo (Dom.Rep.). There he found the flowerbush and called it "Fuchsia triphylla flore coccinea" after Leonhart Fuchs (1501-1566), a German botanist and medic. Perhaps I tell you nothing new, but for me it was just interesting to look it up. Regards Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Robert Richmond Gesendet: Sonntag, 06. Dezember 2009 04:20 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: staining for lipofuscin Worthwhile to get the name of the stuff straight - Lipofuscin - pronounced LIE-po-FUSS-in - from the Latin word fuscus, 'dark' - is the yellow-brown pigment. Often confused with fuchsin - FYOOK-sin - dyes named after the color fuscia (FYOO-sha) which is named after the flower, which is named after somebody named Fuchs (FOOKS). Confusing. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Dec 6 10:50:03 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Dec 6 10:50:08 2009 Subject: SPAM-LOW: [Histonet] OT fuchsia In-Reply-To: <0DCA214C9BFF4E15AAED2AAD18F31CE2@prueggihctechlt> Message-ID: <778745.53218.qm@web65711.mail.ac4.yahoo.com> That you can thank Robert Koch. He was studying TB bacillus in Germany. One day in winter he left the stained slides over a heater. The solution evaporated. Trying to clean them he used acid alcohol. Everything was destained EXCEPT for the TB bacillus. He created that stain. Ren? J. --- On Sun, 12/6/09, Patsy Ruegg wrote: From: Patsy Ruegg Subject: RE: SPAM-LOW: [Histonet] OT fuchsia To: gu.lang@gmx.at, "'Robert Richmond'" Cc: histonet@lists.utsouthwestern.edu Date: Sunday, December 6, 2009, 11:40 AM I love hearing about the history of Histology, I always ponder how things were discovered, like how the heck did someone figure out that if you stained micorganisms with a dye and then treated them with acid they would stay stained (be acid fast).? This motivates me to discover things myself.. I love what we do. Cheers, Patsy? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Sunday, December 06, 2009 1:44 AM To: 'Robert Richmond' Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] OT fuchsia Hi Bob, You made me wondering if your explanation of the flower's name is true, because German Fuchs means fox and I grew up with the believing, that the name is derived from the red colour of the fox. - But I was wrong. I found the history of the exploration on a Fuchsien-website. The discoverer was the Franziskaner-Monk Charles Plumier, born on 20th April 1646 in Marseille. He was sent by Louis XIV to Santa Domingo (Dom.Rep.). There he found the flowerbush and called it "Fuchsia triphylla flore coccinea" after Leonhart Fuchs (1501-1566), a German botanist and medic. Perhaps I tell you nothing new, but for me it was just interesting to look it up. Regards Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Robert Richmond Gesendet: Sonntag, 06. Dezember 2009 04:20 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: staining for lipofuscin Worthwhile to get the name of the stuff straight - Lipofuscin - pronounced LIE-po-FUSS-in - from the Latin word fuscus, 'dark' - is the yellow-brown pigment. Often confused with fuchsin - FYOOK-sin - dyes named after the color fuscia (FYOO-sha) which is named after the flower, which is named after somebody named Fuchs (FOOKS). Confusing. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Sun Dec 6 11:03:25 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Sun Dec 6 11:04:14 2009 Subject: SPAM-LOW: [Histonet] OT fuchsia In-Reply-To: <0DCA214C9BFF4E15AAED2AAD18F31CE2@prueggihctechlt> References: <0DCA214C9BFF4E15AAED2AAD18F31CE2@prueggihctechlt> Message-ID: I'm with Patsy, it's a marvel! I love my field too! I just became a member of Face Book and attached some histology links on my "Wall" with Masimo Tosi's images on it. I have numerous musicians and Fine Art Artists on my friendship list. Below is what I explained to people who wouldn't know what they were seeing, and included is a quote one of my musician friends stated after viewing Masimo's Image of umbilical cord cross-sections on this link http://www.flickr.com/ photos/73366321@N00/ My explanation of the image was: Akemi Allison-TachaOh, by the way, these multiple images are cross- sections of umbilical cords. If you click on any portion of the images, it will zoom in. You can then view the captions. We histologist can see things that most people don't! We can be pretty wacky! After all, we play with dead body parts! Akemi "David KahlRemember, Galileo was considered wacky, Columbus was, too! There are many ways to view the miracles of life, within and without. Keep looking, as you do, in the areas ignored by others and see what they do not. Light brings enlightenment." Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Dec 6, 2009, at 9:40 AM, Patsy Ruegg wrote: > I love hearing about the history of Histology, I always ponder how > things > were discovered, like how the heck did someone figure out that if you > stained micorganisms with a dye and then treated them with acid > they would > stay stained (be acid fast). This motivates me to discover things > myself. > I love what we do. > > Cheers, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Gudrun Lang > Sent: Sunday, December 06, 2009 1:44 AM > To: 'Robert Richmond' > Cc: histonet@lists.utsouthwestern.edu > Subject: SPAM-LOW: [Histonet] OT fuchsia > > Hi Bob, > You made me wondering if your explanation of the flower's name is > true, > because German Fuchs means fox and I grew up with the believing, > that the > name is derived from the red colour of the fox. - But I was wrong. > I found the history of the exploration on a Fuchsien-website. > > The discoverer was the Franziskaner-Monk Charles Plumier, born on > 20th April > 1646 in Marseille. He was sent by Louis XIV to Santa Domingo > (Dom.Rep.). > There he found the flowerbush and called it "Fuchsia triphylla flore > coccinea" after Leonhart Fuchs (1501-1566), a German botanist and > medic. > > Perhaps I tell you nothing new, but for me it was just interesting > to look > it up. > > Regards > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von > Robert > Richmond > Gesendet: Sonntag, 06. Dezember 2009 04:20 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Re: staining for lipofuscin > > Worthwhile to get the name of the stuff straight - > > Lipofuscin - pronounced LIE-po-FUSS-in - from the Latin word fuscus, > 'dark' - is the yellow-brown pigment. > > Often confused with fuchsin - FYOOK-sin - dyes named after the color > fuscia (FYOO-sha) which is named after the flower, which is named > after somebody named Fuchs (FOOKS). > > Confusing. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Sun Dec 6 12:47:55 2009 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Sun Dec 6 12:48:02 2009 Subject: [Histonet] Alcian Blue Message-ID: In the past, when we made up 1% Alcian blue in 3% acetic acid (using bottle distilled water), the pH was always 2.5. We did not have to modify it to get the proper pH. Still using the same powder, we are getting a pH of 2.1. I hate to adjust it because it seems to affect the staining. Any suggestions. The pH meter is calibrated monthly. Diana From rjbuesa <@t> yahoo.com Sun Dec 6 13:14:58 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Dec 6 13:15:03 2009 Subject: [Histonet] Alcian Blue In-Reply-To: Message-ID: <923002.90547.qm@web65713.mail.ac4.yahoo.com> If the powder and the method?are the same, and the pH-meter is calibrated, then look to your distilled water source. That should be "the culprit". If now it?is "deionized" and previously it was "glass distilled", that should account for the difference. Perhaps there is a problem with the "deionizer" used for your water supply. Ren? J. --- On Sun, 12/6/09, Diana McCaig wrote: From: Diana McCaig Subject: [Histonet] Alcian Blue To: histonet@lists.utsouthwestern.edu Date: Sunday, December 6, 2009, 1:47 PM In the past, when we made up 1% Alcian blue in 3% acetic acid (using bottle distilled water), the pH was always 2.5.? We did not have to modify it to get the proper pH.? Still using the same powder, we are getting a pH of 2.1.? I hate to adjust it because it seems to affect the staining.? Any suggestions.? The pH meter is calibrated monthly. Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Dec 6 15:31:30 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Dec 6 15:32:15 2009 Subject: SPAM-LOW: Reponses to Merced and Richard Re: [Histonet] cryojane tape transfer system In-Reply-To: <000101ca6c61$541e4d60$fc5ae820$@callis@bresnan.net> References: <000101ca6c61$541e4d60$fc5ae820$@callis@bresnan.net> Message-ID: <057346ACEE754D0098ECB09C65412359@prueggihctechlt> Here, here Gayle, I have a picture on my website of a calcified horse carpal bone I cut using the Instrumedics tape transfer system, I bet no one would have been able to do that without using the tape. www.ihctech.net Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Monday, November 23, 2009 10:21 AM To: 'Histonet' Cc: emmanuel.mineo@leica-microsystems.com Subject: SPAM-LOW: Reponses to Merced and Richard Re: [Histonet] cryojane tape transfer system You wrote: Unless I'm missing something, I don't understand why people use this tape? It seems like a marketing gimmick to me...ol' fashion' melting of sections onto slides works perfectly for us... ? Regards, Merced Merced, Yes, you are missing something. If you have ever tried to cryosection undecalcified bone or extremely difficult tissues that simply will not result in "ol' fashion' melting" onto a slide , then you would understand why people use this unique cryosectioning system. It is not some "marketing gimmick" but an unique instrument helping many laboratories obtain frozen sections that otherwise are scrunched up, shattered, and destroyed. I suggest you go to the Instrumedics website or www.alphelys.com for a superb slide show and learn how this instrument works before making assumptions about a technology that serves many of us more than well. A happy, informed user of the Cryojane................ Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT As for what Richard wrote: Hi Everybody, I was hoping to get some advice - I'm cryosectioning plant tissues and transferring sections to slides using the Cryojane system. However, i'm having problems in transferring the sections without them falling apart during the tape transfer. I'm fixing my tissue for 24 hours in ethanol:acetic acid (3:1), embedding in O.C.T, snap-freezing and then sectioning at between 2 and 14 microns. The sections seem to be ok but whenever i remove the adhesive tape from the slide a large part of the tissue is removed with it. As a result I lose the majority of my section. I've tried using both 1x and 1/2x slides (CFSA adhesive slides from instrumedics) but neither have given satisfactory results. Does anyone have any suggestions as to how i could reduce the loss of tissue? Any advice would be much appreciated. Thanks Richard Dear Richard, I could be the fixative you are using that causes the problem. If the fixative contains alcohol, the alcohol acts as antifreeze when you try to snap freeze a tissue, animal or plant. The alcohol may cause problems with how the pink tape sticks to the face of the plant tissue, and allows them to fall apart during the tape transfer. If you rinse away the fixative, then you should cryoprotect the fixed plant tissue with 30% sucrose before snap freezing. vbThis will remove the alcohol. If cryoprotection causes problems with the final staining results, then try unfixed plant tissue, snap freeze, Cryojane tape transfer the section and then fix the transferred plant section in your favorite fixative. You may have to optimize the time in fixative though. Other suggestions: Do a double UV flash, but wait for 15 to 20 seconds between flashes. You must allow the UV light source (capacitor) build up enough charge to work properly. This double flash seems to help polymerize the coating more thoroughly, and the section should transfer better. Also, the tape must be removed at an angle across the slide, very slowly, and inside the cryostat (I am sure you probably do this already.) Also, try the 4X slide if you still have problems with 1/2X and 1X slides. You might ask Leica to send you a few to try before investing in a whole box of these. Contact Emmanuel Mineo, Intrumedics Product Manager emmanuel.mineo@leica-microsystems.com for the 4X slides. Manny is a nice gentleman who has worked with Cryojane for many years and has always been helpful to us. Once again, do the double UV light flash with the 4X slides. They are gooey, but may/should hold more securely. With undecalcified bone, we use the 1/2X but do the double flash routinely for all sections. Good luck Gayle Callis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Dec 6 15:48:46 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Dec 6 15:49:29 2009 Subject: SPAM-LOW: [Histonet] zinc or alcoholic formalin In-Reply-To: <4B06996C.6034.00E2.0@mont-hosp.com> References: <20091120180532824@smtp442.redcondor.net> <4B06996C.6034.00E2.0@mont-hosp.com> Message-ID: <838C9558614C405FA897B2C30602EEE9@prueggihctechlt> I use zinc formalin and my process is the same as with formalin, I have heard some people complain that zinc formalin can precipitate in the tissue processor but I have not ever seen that, I have been using zinc formalin for 15 years with no problems. I process a lot of animal tissue so my processing schedule is shorter than for human tissue, for animal I spend only 20 min per station rather than the hour I spend for human tissues. Animal tissue has less fat and can be over processed (it will get hard and dry) easily. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Evanish Sent: Friday, November 20, 2009 11:28 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] zinc or alcoholic formalin Does anyone use zinc formalin or alcoholic formalin on their tissue processors? If so what is your routine run program Chris Montgomery Hospital >>> 11/20/2009 1:05 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Second Call for Manuscripts for Special Issue of Journal of Histotechnology (gayle callis) 2. Preventing slide labeling mistakes (Morken, Tim) 3. Re: Preventing slide labeling mistakes (Lynette Pavelich) 4. Re: Fwd: foxp3 ab ihc (Marilyn Tyler) 5. RE: Preventing slide labeling mistakes (Mike Pence) 6. RE: SPAM-LOW: [Histonet] Re: freezing skeletal muscle (Patsy Ruegg) 7. Re: Preventing slide labeling mistakes (Victor Tobias) 8. RE: Preventing slide labeling mistakes (Morken, Tim) 9. RE: Preventing slide labeling mistakes (Sebree Linda A) 10. RE: Preventing slide labeling mistakes (Armandi, Arlene) 11. CAP guidelines for positive controls (Tiana Fountain) 12. CAP QUESTION ANP.12425 ASR disclaimer on pathology reports (Foshey, Annette) 13. course recommendation (Mike Tighe) 14. Re: CAP QUESTION ANP.12425 ASR disclaimer on pathology reports (Rene J Buesa) 15. AW: [Histonet] Preventing slide labeling mistakes (Gudrun Lang) ---------------------------------------------------------------------- Message: 1 Date: Thu, 19 Nov 2009 16:11:37 -0700 From: "gayle callis" Subject: [Histonet] Second Call for Manuscripts for Special Issue of Journal of Histotechnology To: "Histonet" Message-ID: <002301ca696d$a65f0fc0$f31d2f40$@callis@bresnan.net> Content-Type: text/plain; charset="utf-8" Dear Histonet Colleagues, A second call for manuscripts for Special Issue of Journal of Histotechnology: The Journal of Histotechnology (JOH) editors, Editorial Board, and guest editor Dr. Richard Cartun are pleased to announce a call for manuscripts for the March 2010 special issue of JOH, with focus on Immunohistochemistry and In Situ Hybridization. Technical notes and methodology-focused contributions are particularly encouraged; clinical, research, and veterinary topics are welcome. Manuscripts must be submitted prior to November 30, 2009 in order to be considered for the March issue. Manuscripts may be sent to Ms. Jane Jacobi, Managing Editor, at joh@nsh.org. Authors wishing pre-submission guidance with the writing process and assignment of a writing partner may contact Ms. Gayle Callis, JOH Assistant Editor, at gayle.callis@bresnan.net. Information for authors is detailed at www.nsh.org under Journal of Histotechnology. and an example of a Technical Note format is available upon request from Ms. Callis and NSH office. Gayle M. Callis JOH Assistant Editor National Society for Histotechnology Journal of Histotechnology 10320 Little Patuxent Pkwy #804 Columbia, MD 21044 P: 443-535-4060 E: joh@nsh.org ------------------------------ Message: 2 Date: Thu, 19 Nov 2009 16:20:16 -0800 From: "Morken, Tim" Subject: [Histonet] Preventing slide labeling mistakes To: "histonet@lists.utsouthwestern.edu" Message-ID: <1AAF670737F193429070841C6B2ADD4CF7892093@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA ------------------------------ Message: 3 Date: Thu, 19 Nov 2009 22:52:07 -0500 From: "Lynette Pavelich" Subject: Re: [Histonet] Preventing slide labeling mistakes To: , Message-ID: <4B05CC17020000EE0002E9B9@smtp-gw.hurleymc.com> Content-Type: text/plain; charset=US-ASCII Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 20 Nov 2009 07:52:05 +0200 From: "Marilyn Tyler" Subject: Re: [Histonet] Fwd: foxp3 ab ihc To: "histonet" Message-ID: <4B064AA5020000900008096B@gwiasmtp.uct.ac.za> Content-Type: text/plain; charset=US-ASCII Thanks to all in Histonet world posted on Heathers behalf who is still having problems with her access Marilyn >>> Heather McCleod 2009/11/19 10:43 AM >>> Sorry to bother you Marilyn. Please forward. I have an answer, i.e. confirmation of what I would like to use. Thanks to all who replied to my foxp3 query. Heather >>> "Marilyn Tyler" 2009/11/18 09:47 AM >>> Does anybody in histoland use any FOXP3 antibody. I would like to know which is the better ab to use for FFPE human material. Posting on behalf of Heather as she cannot get through to Histonet >>> Heather McCleod 2009/11/18 08:37 AM >>> Thank you The Histonet ROCKS!!! Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 20 Nov 2009 08:37:53 -0600 From: "Mike Pence" Subject: RE: [Histonet] Preventing slide labeling mistakes To: "Lynette Pavelich" , , Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3CA2@is-e2k3.grhs.net> Content-Type: text/plain; charset="us-ascii" You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 20 Nov 2009 07:52:54 -0700 From: "Patsy Ruegg" Subject: RE: SPAM-LOW: [Histonet] Re: freezing skeletal muscle To: "'Robert Richmond'" , Message-ID: Content-Type: text/plain; charset="us-ascii" Especially for bone, I have found that formalin fixation and then infiltrating in 30% sucrose before freezing the way Samuri described works the very best for me. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, November 19, 2009 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Re: freezing skeletal muscle Galina Deyneko asks about freezing skeletal muscle. I'm not sure that temperature control of the isopentane is too important - it should be very slightly viscous. But do get rid of explosive isopentane and get a non-flammable substitute - see my previous posts on this topic. The technique for freezing skeletal muscle is easier demonstrated than described. Hold a small piece (maybe 5 x 10 mm) in tweezers, and coat it with talc powder so that it appears white on the surface. Then dip the specimen in and out of the isopentane - roughly two dips per second - until it is frozen solid. That will eliminate the ice crystal artifact. But you have to try it a few times before it will quite work for you. Set aside one dead rat and one afternoon. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 20 Nov 2009 07:10:36 -0800 From: Victor Tobias Subject: Re: [Histonet] Preventing slide labeling mistakes To: Lynette Pavelich Cc: histonet@lists.utsouthwestern.edu, Timothy.Morken@ucsfmedctr.org Message-ID: <4B06B16C.2000507@pathology.washington.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Lynette, You might want to also investigate different fonts if that is an option. Keeping the same size but using a different font can make a world of difference. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Lynette Pavelich wrote: > Hi Tim, > We do everything you do, but we also match our blocks to the written > number on the slides. We catch the majority of our mistakes at that > point. The other is because the font on the label is so small, we have > a hard time seeing the difference between 5's & 6's!!! I'm sure it's > not our ages or anything! We're working with CoPath to get a larger > font!! Ahhh, getting old is SO fun! And yes, reading glasses work > pretty good!! LOL > > Have a great weekend everyone!! > Lynette > > Lynette Pavelich, HT(ASCP) > Histology Supervisor > MSH Competency Coordinator > Hurley Medical Center > One Hurley Plaza > Flint, MI 48503 > email: Lpaveli1@hurleymc.com > ph: 810-257-9948 > fax: 810-762-7082 > >>>> "Morken, Tim" 11/19/09 7:20 PM >>> >>>> > Hi, Can people share their procedures for preventing manual slide > labeling mistakes? No need to include barcoding - we are exploring that > but it is a ways off. > > We currently have a manual process: > We prohibit pre-labeling slides in batches (many blocks/slides at one > time), require labeling slides (hand-written) only at the time of > cutting a single block, and matching paper label to the slide after > staining. We don't currently match blocks to slides. > > Thanks for any info! > > Tim Morken > Supervisor, Histology / IPOX > UCSF Medical Center > San Francisco, CA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Fri, 20 Nov 2009 08:41:47 -0800 From: "Morken, Tim" Subject: RE: [Histonet] Preventing slide labeling mistakes To: "Mike Pence" , "Lynette Pavelich" , "histonet@lists.utsouthwestern.edu" Message-ID: <1AAF670737F193429070841C6B2ADD4CF7892156@EXMBMCB15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu; Morken, Tim Subject: RE: [Histonet] Preventing slide labeling mistakes You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 20 Nov 2009 10:55:04 -0600 From: "Sebree Linda A" Subject: RE: [Histonet] Preventing slide labeling mistakes To: "Morken, Tim" , "Mike Pence" , "Lynette Pavelich" , Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF677@UWHC-MAIL01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" If the matching is done at the time of sectioning, i.e. making sure the block you are about to cut matches the slide you have ready to pick up that block's section(s), it will reduce mislabeling errors....and its GLP! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, November 20, 2009 10:42 AM To: Mike Pence; Lynette Pavelich; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Preventing slide labeling mistakes Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu; Morken, Tim Subject: RE: [Histonet] Preventing slide labeling mistakes You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 20 Nov 2009 09:03:08 -0800 From: "Armandi, Arlene" Subject: RE: [Histonet] Preventing slide labeling mistakes To: "Morken, Tim" , "Mike Pence" , "Lynette Pavelich" , Message-ID: Content-Type: text/plain; charset="US-ASCII" One more suggestion. Have you considered using Xylene resistant slide labels? We print the labels at the time we put the blocks in order. We then put the corresponding labels on the trays with the blocks. When the tech picks up a tray, they have the labels ready to put directly onto the slides as they cut. This eliminates the labeling errors resulting from the matching the hand written slides (sometimes illegible) to the labels, after the slides have been stained. Just a thought. Arlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, November 20, 2009 8:42 AM To: Mike Pence; Lynette Pavelich; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Preventing slide labeling mistakes Thanks for the suggestions. One clarification: matching blocks and slides CATCHES mistakes; it does not reduce or eliminate them. Tim -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, November 20, 2009 6:38 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu; Morken, Tim Subject: RE: [Histonet] Preventing slide labeling mistakes You must match your blocks and slides. This will eliminate a lot of your mistakes/ NOT ALL! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, November 19, 2009 9:52 PM To: histonet@lists.utsouthwestern.edu; Timothy.Morken@ucsfmedctr.org Subject: Re: [Histonet] Preventing slide labeling mistakes Hi Tim, We do everything you do, but we also match our blocks to the written number on the slides. We catch the majority of our mistakes at that point. The other is because the font on the label is so small, we have a hard time seeing the difference between 5's & 6's!!! I'm sure it's not our ages or anything! We're working with CoPath to get a larger font!! Ahhh, getting old is SO fun! And yes, reading glasses work pretty good!! LOL Have a great weekend everyone!! Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Morken, Tim" 11/19/09 7:20 PM >>> Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. ------------------------------ Message: 11 Date: Fri, 20 Nov 2009 11:14:23 -0600 From: "Tiana Fountain" Subject: [Histonet] CAP guidelines for positive controls To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello, I am trying to find the guidelines for CAP that describes how long IHC positive control slides are to be kept on file ... does anyone know? Thanks in advance! Tiana -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. ------------------------------ Message: 12 Date: Fri, 20 Nov 2009 11:54:59 -0600 From: "Foshey, Annette" Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports To: " (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain; charset="us-ascii" What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ------------------------------ Message: 13 Date: Fri, 20 Nov 2009 12:57:36 -0500 From: "Mike Tighe" Subject: [Histonet] course recommendation To: Message-ID: <4B069246.26E4.00EE.0@trudeauinstitute.org> Content-Type: text/plain; charset=US-ASCII I am looking for a conference/course to attend in 2010. I am experienced in IF and IHC and would like to learn more about any new techniques and products that might be available. Does anyone have a recommendation for a course or a conference in the US? I am not a certified HT but I pretend to be one:) Thanks for any suggestions!! Mike ------------------------------ Message: 14 Date: Fri, 20 Nov 2009 09:58:17 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports To: "\(histonet@lists.utsouthwestern.edu\)" , AnnetteFoshey Message-ID: <654236.83215.qm@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We used to add that the methods were experimental. Ren? J. --- On Fri, 11/20/09, Foshey, Annette wrote: From: Foshey, Annette Subject: [Histonet] CAP QUESTION ANP.12425 ASR disclaimer on pathology reports To: " (histonet@lists.utsouthwestern.edu)" Date: Friday, November 20, 2009, 12:54 PM What is the current practice for meeting this regulation? Is a general statement (disclaimer) placed on all pathology reports that have immunocytochemistry and/or FISH and ISH or is the disclaimer placed only on the reports where the class 1 ASR antibody or nucleic acid have been used? Thanks for your input? Annette Foshey Charge Tech in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Fri, 20 Nov 2009 18:59:49 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] Preventing slide labeling mistakes To: "'Morken, Tim'" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" We write a "macro-protocol" at the time of grossing with all blocknumbers and additional identifer. This paper is matched the first time with all blocks after embedding and before cutting. Missing blocks or wrong labelled blocks are found. The second time the paper is matched with all slides after staining and before delivering. Missing and wrong labelled slides are found. Additional the slide-number and material is matched with the "case sheet". The slides are labelled batchwise with 10 per batch immediatly before cutting. The identifiers (letters, "lateral", "resection-margin", etc.) are written on side of the cassette, so numbers can be written big enough. The numbers have maximum of 5 digits slash year. 12345/09 Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Morken, Tim Gesendet: Freitag, 20. November 2009 01:20 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Preventing slide labeling mistakes Hi, Can people share their procedures for preventing manual slide labeling mistakes? No need to include barcoding - we are exploring that but it is a ways off. We currently have a manual process: We prohibit pre-labeling slides in batches (many blocks/slides at one time), require labeling slides (hand-written) only at the time of cutting a single block, and matching paper label to the slide after staining. We don't currently match blocks to slides. Thanks for any info! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 72, Issue 22 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpyse <@t> x-celllab.com Mon Dec 7 07:12:01 2009 From: cpyse <@t> x-celllab.com (Cynthia Pyse) Date: Mon Dec 7 07:12:59 2009 Subject: [Histonet] Bachelor's Degrees In-Reply-To: <90354A475B420441B2A0396E5008D4965E30B0@copc-sbs.COPC.local> References: <5a7745af0912051146m18fb7211vd630af69ee975654@mail.gmail.com> <90354A475B420441B2A0396E5008D4965E30B0@copc-sbs.COPC.local> Message-ID: <000001ca773e$dd88fae0$989af0a0$@com> Tom, I agree with you about Nate qualifications. Unfortunately in NYS if you bring in outside work your tech MUST be licensed in NY. The copy of the license need to be displayed in the lab. I haven't found any way to hire employees without a license. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Saturday, December 05, 2009 3:23 PM To: Nathan Jentsch Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bachelor's Degrees One more reason to consider "carefully" before throwing support to state licensure where it does not exist. I feel sorry for you Nathan and I'd like to have someone explain the upside of licensing to you. It seems it's not about having a license (like a driver's license) to practice histology. I fear it's just more about fattening state coffers and adding another level of bureauracracy to things. If you are educated (as you are Nate) and if you are academically eligible to sit for the registry exam. And if you can satisfactorily pass the exam, what has state licensing got to do with it? Are you a better histologist in New York because you're "licensed" as opposed to your neighbors in PA, for example who aren't? I think not. Does licensing prove something that science degrees and registry certifications do not? Maybe I just don't get it. And I'm not trying to pick a fight here with the supporters of licensing. I just haven't heard a good convincing argument for it yet. I'm also quite certain that even though monetary compensation has improved somewhat, the last thing most Histologists need is another payment. The privilege to work in a certain state, which is paid for (by you) nothing more?! I suppose some kindly employers out there somewhere could pay for it...good luck with that. Here's an idea, let's say you're degreed and registry eligible and/or have passed your board exam(s) and are certified. How 'bout the state says you've met the qualification for licensing, here you go! Nate you are degreed and certified and in my book and in the book of the current state I live in - Oregon - and the states I've worked in - Wisconsin, Michigan and Minnesota - you are more than qualified to work. I for one would not hesitate in the least to consider a person such as yourself for employment. Again you are more than qualified, even though you are "unlicensed". I guess I just don't understand how credentialing - degrees and certifications - aren't enough, but licensing is the magic ticket to better science/medicine/patient care/whatever. I'm sure some folks out there will bring on the firestorm, but again Nathan I feel sorry for you and I don't see the reasoning behind this. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nathan Jentsch Sent: Saturday, December 05, 2009 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bachelor's Degrees Paula, Let me tell you that this is an extremely frustrating point for me not for getting a job but for getting a license in New York State (which is related because I'm technically supposed to have a license to work). Despite the fact that I have a B.S. in a science field and have been working competently at my job for almost two years now, the state wants me to have an A.S. in histotechnology to get my license. They won't even consider HT certification as sufficient. If a collective group of experts in the fields of laboratory science and pathology say I'm qualified, why isn't that good enough for a bunch of beurocrats who can't even manage the pocket book of our state. Nate _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rcharles <@t> state.pa.us Mon Dec 7 07:19:13 2009 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Mon Dec 7 07:19:20 2009 Subject: [Histonet] slide handling for special stains Message-ID: <3809C163DC1DA54AA534B3C7794D07B648035E8C4D@ENHBGMBX01.PA.LCL> Hello all, I need some information on special stains in regards to handling of slides. Would it be good practice to deparaffinize and rehydrate slides then allow them to sit in water over night or over the weekend before doing the actual staining? Thanks Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rcharles@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! From histonet.nospam <@t> vneubert.com Mon Dec 7 07:52:36 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Mon Dec 7 07:59:49 2009 Subject: [Histonet] slide handling for special stains In-Reply-To: <3809C163DC1DA54AA534B3C7794D07B648035E8C4D@ENHBGMBX01.PA.LCL> References: <3809C163DC1DA54AA534B3C7794D07B648035E8C4D@ENHBGMBX01.PA.LCL> Message-ID: <4B1D08A4.8090807@vneubert.com> I don't see any point in leaving deparaffinized and rehydrated(!) slides any longer in water. The tissue might even fall off. What special stains are you goint to do? > Hello all, > I need some information on special stains in regards to handling of slides. Would it be good practice to deparaffinize and rehydrate slides then allow them to sit in water over night or over the weekend before doing the actual staining? > Thanks > Roger > > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > rcharles@state.pa.us > > > No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cmiller <@t> physlab.com Mon Dec 7 08:11:01 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Dec 7 08:11:07 2009 Subject: [Histonet] RE: slide handling for special stains In-Reply-To: <3809C163DC1DA54AA534B3C7794D07B648035E8C4D@ENHBGMBX01.PA.LCL> References: <3809C163DC1DA54AA534B3C7794D07B648035E8C4D@ENHBGMBX01.PA.LCL> Message-ID: What would be the point?? Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Monday, December 07, 2009 7:19 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] slide handling for special stains Hello all, I need some information on special stains in regards to handling of slides. Would it be good practice to deparaffinize and rehydrate slides then allow them to sit in water over night or over the weekend before doing the actual staining? Thanks Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rcharles@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Kim.Donadio <@t> bhcpns.org Mon Dec 7 08:15:54 2009 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Mon Dec 7 08:16:23 2009 Subject: [Histonet] slide handling for special stains In-Reply-To: <3809C163DC1DA54AA534B3C7794D07B648035E8C4D@ENHBGMBX01.PA.LCL> Message-ID: I wouldn't make that a habit. It eventually saturates the cells and then they fall off like the previous poster mentioned. Also, especially leaving them in water for a whole weekend you risk contamination. Not good practice. If you absolutely have to leave them in something after they have been deparaffinized I would suggest 95% alcohol. With that said, I think best practice would be to leave them paraffinized until you have time to do the stains. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Charles, Roger" Sent by: histonet-bounces@lists.utsouthwestern.edu 12/07/2009 07:19 AM To "Histonet (histonet@lists.utsouthwestern.edu)" cc Subject [Histonet] slide handling for special stains Hello all, I need some information on special stains in regards to handling of slides. Would it be good practice to deparaffinize and rehydrate slides then allow them to sit in water over night or over the weekend before doing the actual staining? Thanks Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rcharles@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From rjbuesa <@t> yahoo.com Mon Dec 7 08:22:37 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 7 08:22:44 2009 Subject: [Histonet] Bachelor's Degrees In-Reply-To: <000001ca773e$dd88fae0$989af0a0$@com> Message-ID: <637382.81864.qm@web65702.mail.ac4.yahoo.com> Cindy: And not being able to hire anybody without the license is absolutely great and why the license was created in the firs place, namely, to avoid having low paid people doing histology work?in detriment to others more qualified. The salary competition posed by those not licensed is the cause for the low pay histotechs receive when compared with other professionals in the medical laboratory. That was exactly the point: to assure a just salary for histotechs. Ren? J. ? --- On Mon, 12/7/09, Cynthia Pyse wrote: From: Cynthia Pyse Subject: RE: [Histonet] Bachelor's Degrees To: "'Thomas Jasper'" , "'Nathan Jentsch'" Cc: histonet@lists.utsouthwestern.edu Date: Monday, December 7, 2009, 8:12 AM Tom, I agree with you about Nate qualifications. Unfortunately in NYS if you bring in outside work your tech MUST be licensed in NY. The copy of the license need to be displayed in the lab. I haven't found any way to hire employees without a license. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cpyse@x-celllab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Saturday, December 05, 2009 3:23 PM To: Nathan Jentsch Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bachelor's Degrees One more reason to consider "carefully" before throwing support to state licensure where it does not exist.? I feel sorry for you Nathan and I'd like to have someone explain the upside of licensing to you.? It seems it's not about having a license (like a driver's license) to practice histology.? I fear it's just more about fattening state coffers and adding another level of bureauracracy to things. If you are educated (as you are Nate) and if you are academically eligible to sit for the registry exam.? And if you can satisfactorily pass the exam, what has state licensing got to do with it?? Are you a better histologist in New York because you're "licensed" as opposed to your neighbors in PA, for example who aren't?? I think not. Does licensing prove something that science degrees and registry certifications do not?? Maybe I just don't get it.? And I'm not trying to pick a fight here with the supporters of licensing.? I just haven't heard a good convincing argument for it yet.? I'm also quite certain that even though monetary compensation has improved somewhat, the last thing most Histologists need is another payment.? The privilege to work in a certain state, which is paid for (by you) nothing more?!? I suppose some kindly employers out there somewhere could pay for it...good luck with that.? Here's an idea, let's say you're degreed and registry eligible and/or have passed your board exam(s) and are certified.? How 'bout the state says you've met the qualification for licensing, here you go!? Nate you are degreed and certified and in my book and in the book of the current state I live in - Oregon - and the states I've worked in - Wisconsin, Michigan and Minnesota - you are more than qualified to work.? I for one would not hesitate in the least to consider a person such as yourself for employment.? Again you are more than qualified, even though you are "unlicensed". I guess I just don't understand how credentialing - degrees and certifications - aren't enough, but licensing is the magic ticket to better science/medicine/patient care/whatever.? I'm sure some folks out there will bring on the firestorm, but again Nathan I feel sorry for you and I don't see the reasoning behind this. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nathan Jentsch Sent: Saturday, December 05, 2009 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bachelor's Degrees Paula, Let me tell you that this is an extremely frustrating point for me not for getting a job but for getting a license in New York State (which is related because I'm technically supposed to have a license to work). Despite the fact that I have a B.S. in a science field and have been working competently at my job for almost two years now, the state wants me to have an A.S. in histotechnology to get my license.? They won't even consider HT certification as sufficient.? If a collective group of experts in the fields of laboratory science and pathology say I'm qualified, why isn't that good enough for a bunch of beurocrats who can't even manage the pocket book of our state. Nate _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Dec 7 08:24:40 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 7 08:24:44 2009 Subject: [Histonet] slide handling for special stains In-Reply-To: <3809C163DC1DA54AA534B3C7794D07B648035E8C4D@ENHBGMBX01.PA.LCL> Message-ID: <175009.94328.qm@web65711.mail.ac4.yahoo.com> No, it is not a good idea. In many occasions leaving the dewaxed sections in DW for prolonged periods of time can cause the section to peel off (not always, but sometimes). The idea is to dewax and do the special stains immediately after. Ren? J. --- On Mon, 12/7/09, Charles, Roger wrote: From: Charles, Roger Subject: [Histonet] slide handling for special stains To: "Histonet (histonet@lists.utsouthwestern.edu)" Date: Monday, December 7, 2009, 8:19 AM Hello all, I need some information on special stains in regards to handling of slides.???Would it be good practice to deparaffinize and rehydrate slides then allow them to sit in water over night or over the weekend before doing the actual staining? Thanks Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rcharles@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Mon Dec 7 08:51:12 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Dec 7 08:51:17 2009 Subject: [Histonet] opinion please Message-ID: <897005.49235.qm@web50308.mail.re2.yahoo.com> Help! My refrigerator died over the weekend.? This fridge had most of my antibodies and IHC reagents.? When I came in, the temperature inside the fridge was a balmy 37C! Do you think any of my reagents are still usable? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From Kim.Donadio <@t> bhcpns.org Mon Dec 7 08:59:33 2009 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Mon Dec 7 09:00:00 2009 Subject: [Histonet] opinion please In-Reply-To: <897005.49235.qm@web50308.mail.re2.yahoo.com> Message-ID: Amazing! How hot do you keep the room temp there? I would toss them, one false test on a patient is more valuable than all those lost reagents. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Kim Merriam Sent by: histonet-bounces@lists.utsouthwestern.edu 12/07/2009 08:51 AM To Histonet cc Subject [Histonet] opinion please Help! My refrigerator died over the weekend. This fridge had most of my antibodies and IHC reagents. When I came in, the temperature inside the fridge was a balmy 37C! Do you think any of my reagents are still usable? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From sfp1968 <@t> gmail.com Mon Dec 7 09:09:04 2009 From: sfp1968 <@t> gmail.com (Terry OBrien) Date: Mon Dec 7 09:09:12 2009 Subject: [Histonet] CM 3600 Message-ID: Looking for someone close to Pittsburgh, PA (or within a 300 miles) with a Leica CM3600 that I could come by and look at. Thanks, Terry From rjbuesa <@t> yahoo.com Mon Dec 7 09:21:45 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 7 09:21:48 2009 Subject: [Histonet] opinion please In-Reply-To: <897005.49235.qm@web50308.mail.re2.yahoo.com> Message-ID: <396784.16683.qm@web65711.mail.ac4.yahoo.com> Perhaps yes or perhaps no, that is the problem. >From now on you cannot know if something that did not work was because of the fridge malfunction. Advise? (Costly advise?): discard everything and get an alarm system for your fridge after you repair it (or get a new one).. Ren? J. --- On Mon, 12/7/09, Kim Merriam wrote: From: Kim Merriam Subject: [Histonet] opinion please To: "Histonet" Date: Monday, December 7, 2009, 9:51 AM Help! My refrigerator died over the weekend.? This fridge had most of my antibodies and IHC reagents.? When I came in, the temperature inside the fridge was a balmy 37C! Do you think any of my reagents are still usable? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Mon Dec 7 09:36:08 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Mon Dec 7 09:36:44 2009 Subject: [Histonet] opinion please Message-ID: I agree with Renee. It will proabaly be at least as costly to have to re-validate everything! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> Rene J Buesa 12/7/2009 11:21 AM >>> Perhaps yes or perhaps no, that is the problem. >From now on you cannot know if something that did not work was because of the fridge malfunction. Advise? (Costly advise?): discard everything and get an alarm system for your fridge after you repair it (or get a new one).. Ren? J. --- On Mon, 12/7/09, Kim Merriam wrote: From: Kim Merriam Subject: [Histonet] opinion please To: "Histonet" Date: Monday, December 7, 2009, 9:51 AM Help! My refrigerator died over the weekend. This fridge had most of my antibodies and IHC reagents. When I came in, the temperature inside the fridge was a balmy 37C! Do you think any of my reagents are still usable? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From TJJ <@t> stowers.org Mon Dec 7 09:38:25 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Dec 7 09:38:35 2009 Subject: [Histonet] FW: OT fuchsia Message-ID: One of the researchers here summed it up nicely when he reported that most discoveries in the lab are not heralded with cries of "Eureka!", but rather with "hmmm....now that's interesting..." Teri Johnson, HT(ASCP)QIHC Stowers Institute for Medical Research Kansas City, MO From carrolpb <@t> umdnj.edu Mon Dec 7 09:38:54 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Dec 7 09:39:04 2009 Subject: [Histonet] opinion please In-Reply-To: References: Message-ID: <4B1D218E.9030303@umdnj.edu> Seriously... you wouldn't drink milk that had sat in a warm fridge all weekend... but you use reagents and antibodies that had? Greg Dobbin wrote: > I agree with Renee. It will proabaly be at least as costly to have to re-validate everything! > Greg > > Greg Dobbin, R.T. > Chief Technologist, Anatomic Pathology > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > "I find that the harder I work, the > more luck I seem to have." > - Thomas Jefferson > > > >>>> Rene J Buesa 12/7/2009 11:21 AM >>> >>>> > Perhaps yes or perhaps no, that is the problem. > >From now on you cannot know if something that did not work was because of the fridge malfunction. > Advise? (Costly advise?): discard everything and get an alarm system for your fridge after you repair it (or get a new one).. > Ren? J. > > --- On Mon, 12/7/09, Kim Merriam wrote: > > > From: Kim Merriam > Subject: [Histonet] opinion please > To: "Histonet" > Date: Monday, December 7, 2009, 9:51 AM > > > Help! > > My refrigerator died over the weekend. This fridge had most of my antibodies and IHC reagents. When I came in, the temperature inside the fridge was a balmy 37C! > > Do you think any of my reagents are still usable? > > Kim > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------- > > Statement of Confidentiality > > This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Laura.Miller <@t> leica-microsystems.com Mon Dec 7 10:00:55 2009 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Mon Dec 7 10:01:03 2009 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 12/07/2009 and will not return until 12/08/2009. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From jbdg <@t> u.washington.edu Mon Dec 7 10:42:01 2009 From: jbdg <@t> u.washington.edu (Josephine Garcia) Date: Mon Dec 7 10:42:31 2009 Subject: [Histonet] Overstaining - Mayers H&E Message-ID: Hi all, My (frozen-section, fixed) slides are coming out much too dark (overstained purple) and I'm not sure why. They are 15-20 micrometer slices of rat gastrocnemius muscle. Can someone please look over our current protocol and tell me what I'm doing wrong? Thanks! Here it is: 1. Perfuse animal with 4% paraformaldehyde fixative. 2. Soak in 0.4% paraformaldehyde 3. Sucrose cycle (5% rinse, 10%, 20%, 30% soak) 4. Embed in OCT, Frozen sections (15-20 micrometers) 5. Let dry for 15-30 min 6. Stain as follows: - Distilled H2O (quick dip) - Mayer's Hematoxylin - 1min (originally we were dipping for 5-10 minutes. I slowly reduced the time to 2min, then 1min, then 30s... still overstained!) - Running lukewarm tap water - drain and continuously fill - 15min or until water runs clear - Distilled H2O (quick dip) - 80% EtOH - 1-2min - Eosin - 2 min - 95% EtOH I - 10sec - 95% EtOH II - 10sec - 100% EtOH I - 10sec - 100% EtOH II - 10sec - Xylene I - 2min - Xylene II - 2min 7. Coverslip and let dry overnight From Janet.Bonner <@t> FLHOSP.ORG Mon Dec 7 10:44:08 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon Dec 7 10:45:55 2009 Subject: SPAM-LOW: [Histonet] OT fuchsia References: <778745.53218.qm@web65711.mail.ac4.yahoo.com> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2BED@fhosxchmb006.ADVENTISTCORP.NET> The Moral here is, -don't just automatically toss your perceived mistakes, look at them and see what happened! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Sun 12/6/2009 11:50 AM To: gu.lang@gmx.at; 'Robert Richmond'; Patsy Ruegg Cc: histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] OT fuchsia That you can thank Robert Koch. He was studying TB bacillus in Germany. One day in winter he left the stained slides over a heater. The solution evaporated. Trying to clean them he used acid alcohol. Everything was destained EXCEPT for the TB bacillus. He created that stain. Ren? J. --- On Sun, 12/6/09, Patsy Ruegg wrote: From: Patsy Ruegg Subject: RE: SPAM-LOW: [Histonet] OT fuchsia To: gu.lang@gmx.at, "'Robert Richmond'" Cc: histonet@lists.utsouthwestern.edu Date: Sunday, December 6, 2009, 11:40 AM I love hearing about the history of Histology, I always ponder how things were discovered, like how the heck did someone figure out that if you stained micorganisms with a dye and then treated them with acid they would stay stained (be acid fast). This motivates me to discover things myself.. I love what we do. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Sunday, December 06, 2009 1:44 AM To: 'Robert Richmond' Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] OT fuchsia Hi Bob, You made me wondering if your explanation of the flower's name is true, because German Fuchs means fox and I grew up with the believing, that the name is derived from the red colour of the fox. - But I was wrong. I found the history of the exploration on a Fuchsien-website. The discoverer was the Franziskaner-Monk Charles Plumier, born on 20th April 1646 in Marseille. He was sent by Louis XIV to Santa Domingo (Dom.Rep.). There he found the flowerbush and called it "Fuchsia triphylla flore coccinea" after Leonhart Fuchs (1501-1566), a German botanist and medic. Perhaps I tell you nothing new, but for me it was just interesting to look it up. Regards Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Robert Richmond Gesendet: Sonntag, 06. Dezember 2009 04:20 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: staining for lipofuscin Worthwhile to get the name of the stuff straight - Lipofuscin - pronounced LIE-po-FUSS-in - from the Latin word fuscus, 'dark' - is the yellow-brown pigment. Often confused with fuchsin - FYOOK-sin - dyes named after the color fuscia (FYOO-sha) which is named after the flower, which is named after somebody named Fuchs (FOOKS). Confusing. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From lpaveli1 <@t> hurleymc.com Mon Dec 7 10:49:29 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Mon Dec 7 10:49:49 2009 Subject: [Histonet] slide handling for special stains In-Reply-To: <175009.94328.qm@web65711.mail.ac4.yahoo.com> References: <3809C163DC1DA54AA534B3C7794D07B648035E8C4D@ENHBGMBX01.PA.LCL> <175009.94328.qm@web65711.mail.ac4.yahoo.com> Message-ID: <4B1CEBC9.59CD.00EE.0@hurleymc.com> My teacher, long retired Miss Mavis Marie, a MT that loved Histology, taught us that if we needed to do the staining the next day, to leave them in 70% alcohol. We did find that sometimes, not always, if left in distilled H2O overnight, it would wash off, but never did when left in 70%. Hope this helped, Lynette >>> Rene J Buesa 12/7/2009 9:24 AM >>> No, it is not a good idea. In many occasions leaving the dewaxed sections in DW for prolonged periods of time can cause the section to peel off (not always, but sometimes). The idea is to dewax and do the special stains immediately after. Ren? J. --- On Mon, 12/7/09, Charles, Roger wrote: From: Charles, Roger Subject: [Histonet] slide handling for special stains To: "Histonet (histonet@lists.utsouthwestern.edu)" Date: Monday, December 7, 2009, 8:19 AM Hello all, I need some information on special stains in regards to handling of slides. Would it be good practice to deparaffinize and rehydrate slides then allow them to sit in water over night or over the weekend before doing the actual staining? Thanks Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rcharles@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rick.Garnhart <@t> memorialhealthsystem.com Mon Dec 7 11:15:05 2009 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Mon Dec 7 11:15:07 2009 Subject: [Histonet] Glypican 3 In-Reply-To: Message-ID: Anyone out there know were Glypican 3 antibody for IHC may be purchased from. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. From rjbuesa <@t> yahoo.com Mon Dec 7 11:24:04 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 7 11:24:08 2009 Subject: [Histonet] Overstaining - Mayers H&E In-Reply-To: Message-ID: <901986.79586.qm@web65710.mail.ac4.yahoo.com> You have to consider that the thicker the section the more intense the staining will be. If you are using now 1 minute and they are still too dark, reduce the staining time?even further. Otherwise I don't see anything wrong with the rest of the protocol. Ren? J. --- On Mon, 12/7/09, Josephine Garcia wrote: From: Josephine Garcia Subject: [Histonet] Overstaining - Mayers H&E To: histonet@lists.utsouthwestern.edu Date: Monday, December 7, 2009, 11:42 AM Hi all, My (frozen-section, fixed) slides are coming out much too dark (overstained purple) and I'm not sure why. They are 15-20 micrometer slices of rat gastrocnemius muscle. Can someone please look over our current protocol and tell me what I'm doing wrong? Thanks! Here it is: 1. Perfuse animal with 4% paraformaldehyde fixative. 2. Soak in 0.4% paraformaldehyde 3. Sucrose cycle (5% rinse, 10%, 20%, 30% soak) 4. Embed in OCT, Frozen sections (15-20 micrometers) 5. Let dry for 15-30 min 6. Stain as follows: - Distilled H2O (quick dip) - Mayer's Hematoxylin - 1min (originally we were dipping for 5-10 minutes. I slowly reduced the time to 2min, then 1min, then 30s... still overstained!) - Running lukewarm tap water - drain and continuously fill - 15min or until water runs clear - Distilled H2O (quick dip) - 80% EtOH - 1-2min - Eosin - 2 min - 95% EtOH I - 10sec - 95% EtOH? II - 10sec - 100% EtOH I - 10sec - 100% EtOH II - 10sec - Xylene I - 2min - Xylene II - 2min 7. Coverslip and let dry overnight _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Dec 7 11:44:30 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Dec 7 11:50:00 2009 Subject: [Histonet] Glypican 3 In-Reply-To: References: , Message-ID: Cell Marque , has a concentrated and a Ready to use. They both work great. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rick.Garnhart@memorialhealthsystem.com [Rick.Garnhart@memorialhealthsystem.com] Sent: Monday, December 07, 2009 12:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Glypican 3 Anyone out there know were Glypican 3 antibody for IHC may be purchased from. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Mon Dec 7 12:01:07 2009 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Dec 7 12:01:12 2009 Subject: [Histonet] FW: Coarse abrasive Message-ID: <5680DA93771F0C48954CC8D38425E72401AB34F9@ISMAIL.parrishmed.local> ________________________________ From: Hannen, Valerie Sent: Friday, December 04, 2009 9:43 AM To: 'histonet@list.utsouthwestern.edu' Subject: Coarse abrasive Help!! My section chief just got word that Leica is no longer producing coarse abrasive. We only have steel blades in our department and we are SO dependent on the abrasives. She does not want to switch to disposable blades. Is there anyone out there in Histo-land who might know a solution to our dilema. Thanks in advance for your answers. Valerie Hannen,MLT(ASCP),HTL,SU(FL) Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32796 ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From Jan.Minshew <@t> leica-microsystems.com Mon Dec 7 12:23:01 2009 From: Jan.Minshew <@t> leica-microsystems.com (Jan.Minshew@leica-microsystems.com) Date: Mon Dec 7 12:22:59 2009 Subject: [Histonet] FW: Coarse abrasive In-Reply-To: <5680DA93771F0C48954CC8D38425E72401AB34F9@ISMAIL.parrishmed.local> Message-ID: Hello Valerie, I believe you may have been given misinformation regarding the knife sharpening abrasives. You can order through Leica Microsystems using catalog number 14041819700 for a single bottle or 14937000 for a case of 6 bottles. Please feel free to contact me if you have any questions. Best wishes, Jan Minshew, HT/HTL(ASCP) Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 800.248.0123 Toll Free 847.405.7051 Direct 847.405.6560 Fax www.leica-microsystems.com Click Here for this month's special offers! "Hannen, Valerie" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] FW: Coarse abrasive 12/07/2009 12:01 PM ________________________________ From: Hannen, Valerie Sent: Friday, December 04, 2009 9:43 AM To: 'histonet@list.utsouthwestern.edu' Subject: Coarse abrasive Help!! My section chief just got word that Leica is no longer producing coarse abrasive. We only have steel blades in our department and we are SO dependent on the abrasives. She does not want to switch to disposable blades. Is there anyone out there in Histo-land who might know a solution to our dilema. Thanks in advance for your answers. Valerie Hannen,MLT(ASCP),HTL,SU(FL) Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32796 ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From llewllew <@t> shaw.ca Mon Dec 7 12:33:03 2009 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Mon Dec 7 12:56:27 2009 Subject: [Histonet] Overstaining - Mayers H&E In-Reply-To: References: Message-ID: There doesn't appear to be anything wrong with the staining schedule. Mayer published more than one alum hematoxylin and one of them is a strong regressive type, and would produce the staining you see. Is it possible that has been used instead of his progressive one? If it has, make the following diluent. Potassium or ammonium alum 50 g Citric acid 1 g or glacial acetic acid 20 mL Distilled water 1L Then dilute the strong alum hematoxylin 1 part with 3 parts diluent. Or, Immediately before the 80% ethanol prior to the eosin, place into a solution of 0.5% acid ethanol for a few seconds. Wash well to blue, and carry on. If that doesn't correct it, then make up a fresh batch of Mayer's hemalum. Bryan Llewellyn ----- Original Message ----- From: "Josephine Garcia" To: Sent: Monday, December 07, 2009 8:42 AM Subject: [Histonet] Overstaining - Mayers H&E > Hi all, > > My (frozen-section, fixed) slides are coming out much too dark > (overstained > purple) and I'm not sure why. They are 15-20 micrometer slices of rat > gastrocnemius muscle. Can someone please look over our current protocol > and > tell me what I'm doing wrong? Thanks! Here it is: > > 1. Perfuse animal with 4% paraformaldehyde fixative. > 2. Soak in 0.4% paraformaldehyde > 3. Sucrose cycle (5% rinse, 10%, 20%, 30% soak) > 4. Embed in OCT, Frozen sections (15-20 micrometers) > 5. Let dry for 15-30 min > 6. Stain as follows: > > - Distilled H2O (quick dip) > - Mayer's Hematoxylin - 1min (originally we were dipping for 5-10 minutes. > I > slowly reduced the time to 2min, then 1min, then 30s... still > overstained!) > - Running lukewarm tap water - drain and continuously fill - 15min or > until > water runs clear > - Distilled H2O (quick dip) > - 80% EtOH - 1-2min > - Eosin - 2 min > - 95% EtOH I - 10sec > - 95% EtOH II - 10sec > - 100% EtOH I - 10sec > - 100% EtOH II - 10sec > - Xylene I - 2min > - Xylene II - 2min > > 7. Coverslip and let dry overnight > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jerry.santiago <@t> jax.ufl.edu Mon Dec 7 13:17:07 2009 From: jerry.santiago <@t> jax.ufl.edu (Santiago, Jerry) Date: Mon Dec 7 13:17:15 2009 Subject: [Histonet] FL Supervisor License In-Reply-To: <8CC41905ADA46DF-44FC-8136@webmail-d088.sysops.aol.com> References: <8CC41905ADA46DF-44FC-8136@webmail-d088.sysops.aol.com> Message-ID: <816DC61E1730E843A0BCF4CCFA1EFCF30148F560@jaxmail.umc.ufl.edu> Marie, If the person is licensed as a technologist with no ASCP certification but at least 5 years of experience, he/she qualifies under rule 64B3-5.002(C)(3). See below. 64B3-5.002 Supervisor. Qualifications and Responsibilities. (1) Qualification. Degrees or semester hours of academic credit required in this section shall be obtained at a regionally accredited college or university or by foreign education equated pursuant to subsection 64B3-6.002(6), F.A.C. In order to be licensed as a supervisor, an applicant shall be licensed or meet the requirements for licensure as a technologist, have a Board approved 2-hour course relating to the prevention of medical errors, which shall include root-cause analysis, error reduction and prevention, patient safety, complete an educational course acceptable to the Department on human immunodeficiency virus and acquired immune deficiency syndrome, and one of the following: (c) Histology Route 1 Five years of pertinent clinical laboratory experience in histology and 25 hours of Board-approved continuing education in supervision and administration within the previous 5 years and HTL (ASCP) Route 2 Five years of pertinent clinical laboratory experience post-certification and 48 hours of Board-approved continuing education in supervision and administration within the previous 5 years and HT (ASCP) Route 3 Five years of pertinent clinical laboratory experience, and 48 hours of Board-approved continuing education in supervision and administration within the previous 5 years, and licensure as a technologist in the specialty of histology Jerry Santiago, BS, HTL(ASCP)QIHC Shands Jacksonville 655 W 8th Street Jacksonville, FL 32209 Tel: 904-244-6149 E-mail: jerry.santiago@jax.ufl.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histosprv06@aol.com Sent: Wednesday, December 02, 2009 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FL Supervisor License This may have been covered already, please accept my apologies for a repeat question. I was curious if anyone knows if you can upgrade a technologist (FL state license only, grandfathered in so NO ASCP) license WITHOUT a 4 year degree? The work experience is 10+ years but this person does NOT have a 2 or 4 year degree of any kind. I know the laws constantly change, was just curious about the current ones, I am not familiar with ASCP guidelines for FL. Thank you in advance. Marie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Mon Dec 7 15:32:42 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Mon Dec 7 15:33:23 2009 Subject: [Histonet] opinion please In-Reply-To: <897005.49235.qm@web50308.mail.re2.yahoo.com> References: <897005.49235.qm@web50308.mail.re2.yahoo.com> Message-ID: <1AAF670737F193429070841C6B2ADD4CF7A4DC88@EXMBMCB15.ucsfmedicalcenter.org> Kim, Test a random sampling (10%) of the antibodies and see if they work. If so, then they are most likely fine. Document the test. Antibodies are very robust and the warmup probably won't damage many, if any, of them. BTW, When I worked for an antibody manufacturer I did a test once as part of a stability problem investigation and left six different antibodies in a 37C oven for up to 4 months. We tested a sample of each weekly. All worked fine and some even worked better even after 4 months! The issue I would be concerned about is bacterial growth in any of them. Keep an eye on that. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Monday, December 07, 2009 6:51 AM To: Histonet Subject: [Histonet] opinion please Help! My refrigerator died over the weekend.? This fridge had most of my antibodies and IHC reagents.? When I came in, the temperature inside the fridge was a balmy 37C! Do you think any of my reagents are still usable? Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine-walters <@t> uiowa.edu Mon Dec 7 15:38:50 2009 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Mon Dec 7 15:38:56 2009 Subject: [Histonet] plasminogen activator inhibitor 1 (PAI 1) antibody Message-ID: Has anyone had any experience with this antibody? There are many companies selling them and I'm not sure which would be best for my paraffin-embedded human tissue? Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walters@uiowa.edu www.uiowa.edu/~cemrf From akemiat3377 <@t> yahoo.com Mon Dec 7 18:02:40 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Mon Dec 7 18:03:32 2009 Subject: [Histonet] opinion please In-Reply-To: <1AAF670737F193429070841C6B2ADD4CF7A4DC88@EXMBMCB15.ucsfmedicalcenter.org> References: <897005.49235.qm@web50308.mail.re2.yahoo.com> <1AAF670737F193429070841C6B2ADD4CF7A4DC88@EXMBMCB15.ucsfmedicalcenter.org> Message-ID: <4B88E048-095D-45CD-AFA9-A9F63F2A4F6F@yahoo.com> I agree with Tim. This is a very common practice for antibody manufacturer's. Also, manufacturer's will revalidate antibodies when they are close to their exploration date and if that antibody has the same sensitivity. They will extend the shelf-life date. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Dec 7, 2009, at 2:32 PM, Morken, Tim wrote: > Kim, > > Test a random sampling (10%) of the antibodies and see if they > work. If so, then they are most likely fine. Document the test. > Antibodies are very robust and the warmup probably won't damage > many, if any, of them. > > BTW, When I worked for an antibody manufacturer I did a test once > as part of a stability problem investigation and left six different > antibodies in a 37C oven for up to 4 months. We tested a sample of > each weekly. All worked fine and some even worked better even after > 4 months! > > The issue I would be concerned about is bacterial growth in any of > them. Keep an eye on that. > > Tim Morken > Supervisor, Histology / IPOX > UCSF Medical Center > San Francisco, CA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam > Sent: Monday, December 07, 2009 6:51 AM > To: Histonet > Subject: [Histonet] opinion please > > Help! > > My refrigerator died over the weekend. This fridge had most of my > antibodies and IHC reagents. When I came in, the temperature > inside the fridge was a balmy 37C! > > Do you think any of my reagents are still usable? > > Kim > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Mon Dec 7 18:11:02 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Mon Dec 7 18:11:51 2009 Subject: [Histonet] opinion please In-Reply-To: <4B88E048-095D-45CD-AFA9-A9F63F2A4F6F@yahoo.com> References: <897005.49235.qm@web50308.mail.re2.yahoo.com> <1AAF670737F193429070841C6B2ADD4CF7A4DC88@EXMBMCB15.ucsfmedicalcenter.org> <4B88E048-095D-45CD-AFA9-A9F63F2A4F6F@yahoo.com> Message-ID: <3EFDA94E-6745-452C-8E48-C2106339C724@yahoo.com> Sorry I made a type "O" I meant to say Also, manufacturer's will revalidate antibodies when they are close to their expiration date and if that antibody has the same sensitivity. They will extend the shelf-life date. > Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Dec 7, 2009, at 5:02 PM, Akemi Allison wrote: > > I agree with Tim. This is a very common practice for antibody > manufacturer's. Also, manufacturer's will revalidate antibodies > when they are close to their exploration date and if that antibody > has the same sensitivity. They will extend the shelf-life date. > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > On Dec 7, 2009, at 2:32 PM, Morken, Tim wrote: > >> Kim, >> >> Test a random sampling (10%) of the antibodies and see if they >> work. If so, then they are most likely fine. Document the test. >> Antibodies are very robust and the warmup probably won't damage >> many, if any, of them. >> >> BTW, When I worked for an antibody manufacturer I did a test once >> as part of a stability problem investigation and left six >> different antibodies in a 37C oven for up to 4 months. We tested a >> sample of each weekly. All worked fine and some even worked better >> even after 4 months! >> >> The issue I would be concerned about is bacterial growth in any of >> them. Keep an eye on that. >> >> Tim Morken >> Supervisor, Histology / IPOX >> UCSF Medical Center >> San Francisco, CA >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >> bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam >> Sent: Monday, December 07, 2009 6:51 AM >> To: Histonet >> Subject: [Histonet] opinion please >> >> Help! >> >> My refrigerator died over the weekend. This fridge had most of my >> antibodies and IHC reagents. When I came in, the temperature >> inside the fridge was a balmy 37C! >> >> Do you think any of my reagents are still usable? >> >> Kim >> Kim Merriam, MA, HT(ASCP)QIHC >> Cambridge, MA >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From k.young1 <@t> uq.edu.au Mon Dec 7 18:15:30 2009 From: k.young1 <@t> uq.edu.au (Karen Young) Date: Mon Dec 7 18:17:32 2009 Subject: [Histonet] Mircom 505 E Cryostat "0000" Message-ID: <5917E3FA58839D40B41CF5B83A68105403AD8E0D@UQEXMB2.soe.uq.edu.au> Hi, We have a microm 505 E cryostat in the lab, the microtome part of which a repairer came to clean. Since the return of the microtome to the cryostat, when we turn it on we no longer get any of the error messages specified in the manual (such as those that come up when the cryostat has been off for a while and is not cool), but rather the displays show "0000". The blank reset button does not work and nor do any of the other buttons. I can find no reference to this display in our manual. Does anyone know what "0000" means or how it can be fixed? Many Thanks, Karen. PhD Candidate School of Biological Sciences University of Queensland Goddard Building (8), Rm 363a From baderbo <@t> gmail.com Mon Dec 7 18:24:51 2009 From: baderbo <@t> gmail.com (Bader Siddiki) Date: Mon Dec 7 18:24:57 2009 Subject: [Histonet] opinion please In-Reply-To: <4B88E048-095D-45CD-AFA9-A9F63F2A4F6F@yahoo.com> References: <897005.49235.qm@web50308.mail.re2.yahoo.com> <1AAF670737F193429070841C6B2ADD4CF7A4DC88@EXMBMCB15.ucsfmedicalcenter.org> <4B88E048-095D-45CD-AFA9-A9F63F2A4F6F@yahoo.com> Message-ID: <3c7e700912071624n5ff96448odfd47f8481385b51@mail.gmail.com> Hello I have the same experience. When I worked for a company and right after 9/11, we shipped antibodies to Israel via Post office at ambient temp. This package came back after 6-8 weeks or may be longer. We do not know how they were stored at post office. We tested the antibodies and could not find differences in the activity using IHC. Bader On Mon, Dec 7, 2009 at 4:02 PM, Akemi Allison wrote: > > I agree with Tim. This is a very common practice for antibody > manufacturer's. Also, manufacturer's will revalidate antibodies when they > are close to their exploration date and if that antibody has the same > sensitivity. They will extend the shelf-life date. > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3377@yahoo.com > > On Dec 7, 2009, at 2:32 PM, Morken, Tim wrote: > > Kim, >> >> Test a random sampling (10%) of the antibodies and see if they work. If >> so, then they are most likely fine. Document the test. Antibodies are very >> robust and the warmup probably won't damage many, if any, of them. >> >> BTW, When I worked for an antibody manufacturer I did a test once as part >> of a stability problem investigation and left six different antibodies in a >> 37C oven for up to 4 months. We tested a sample of each weekly. All worked >> fine and some even worked better even after 4 months! >> >> The issue I would be concerned about is bacterial growth in any of them. >> Keep an eye on that. >> >> Tim Morken >> Supervisor, Histology / IPOX >> UCSF Medical Center >> San Francisco, CA >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >> bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam >> Sent: Monday, December 07, 2009 6:51 AM >> To: Histonet >> Subject: [Histonet] opinion please >> >> Help! >> >> My refrigerator died over the weekend. This fridge had most of my >> antibodies and IHC reagents. When I came in, the temperature inside the >> fridge was a balmy 37C! >> >> Do you think any of my reagents are still usable? >> >> Kim >> Kim Merriam, MA, HT(ASCP)QIHC >> Cambridge, MA >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Gr??en / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten ?????? Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience corp. Phone: + 425 367 4601 Phone: + 425 514 3761 Fax: + 425 367 4817 cell (mobile) phone: + 425 314 0199 e-mail address: baderbo@gmail.com Web site: www.immunobioscience.com Marketing: phone: + 650 343 IBSC (4272) E-mail: anitaIBSC@aol.com From njblademaster <@t> gmail.com Mon Dec 7 20:27:14 2009 From: njblademaster <@t> gmail.com (Nathan Jentsch) Date: Mon Dec 7 20:27:39 2009 Subject: [Histonet] re: bachelor's degrees Message-ID: <5a7745af0912071827v402fc17ah98695db15cbb269f@mail.gmail.com> Rene makes an excellent point as to the reason for having a license. I agree that it is a good idea. Unfortunately NYS took the idea and botched it. The NSH tried to push them in the direction of using the HT exam as their qualifying exam, but instead they want to write their own and they are placing unrealistic education requirements. There is only one school in NYS to get an A.S. in histotechnology, and it would require most people in the state to relocate well away from where they have established their careers. I don't think the bureaucrats realize that they are making it difficult for labs to fill positions, and this will only get worse as people retire from the field and there will only be a handful of fresh graduates every year. As it stands now, I will be taking an online program through Indiana University starting in August in order to meet the requirements set for me. Hopefully they will grant me a limited license until I finish the program so that I can continue to work. Nathan Jentsch B.S. Environmental Science Rochester Institute of Technology From jkiernan <@t> uwo.ca Mon Dec 7 23:27:59 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Dec 7 23:28:04 2009 Subject: pH meter calibration. Was Re: [Histonet] Alcian Blue Message-ID: A pH meter must be calibrated hourly or daily, not monthly! In a research environment, with frequently changing chemicals that might damage the glass electrode, you need to calibrate against standard (usually bought) buffers several times within every day. pH meter electrodes are horribly fragile and horribly expensive. Replacing them is a big item in every lab's budget. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Diana McCaig Date: Sunday, December 6, 2009 13:49 Subject: [Histonet] Alcian Blue To: histonet@lists.utsouthwestern.edu > In the past, when we made up 1% Alcian blue in 3% acetic acid (using > bottle distilled water), the pH was always 2.5. We did not > have to > modify it to get the proper pH. Still using the same > powder, we are > getting a pH of 2.1. I hate to adjust it because it seems > to affect the > staining. Any suggestions. The pH meter is > calibrated monthly. > > Diana > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Tue Dec 8 00:36:43 2009 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Dec 8 00:36:48 2009 Subject: [Histonet] paper on osteoclasts Message-ID: Hi all, I am going crazy - and its not even friday yet! A little while ago (OK about 3 years) i came across a paper where the researcher had shown the transition of macrophages to osteoclasts using immuno staining - i cannot seem to find it on Pubmed, dont know the author date or journal. i hope soemone out there can help best egards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From 41dmb41 <@t> gmail.com Tue Dec 8 07:18:19 2009 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Tue Dec 8 07:18:43 2009 Subject: [Histonet] paper on osteoclasts In-Reply-To: References: Message-ID: Is this it? http://www.jbmronline.org/doi/full/10.1359/jbmr.2000.15.8.1477?cookieSet=1 I've never read the paper, so I don't know exactly what you're looking for... but I sometimes find Google Scholar to be a better search engine for journal articles (which is what I used to find the above article). Drew On Tue, Dec 8, 2009 at 01:36, louise renton wrote: > Hi all, > > I am going crazy - and its not even friday yet! A little while ago (OK about > 3 years) i came across a paper where the researcher had shown the transition > of macrophages to osteoclasts ?using immuno staining - i cannot seem to find > it on Pubmed, dont know the author date or journal. > > i hope soemone out there can help > > best egards > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From 41dmb41 <@t> gmail.com Tue Dec 8 07:19:23 2009 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Tue Dec 8 07:19:48 2009 Subject: [Histonet] paper on osteoclasts In-Reply-To: References: Message-ID: I just realized that link didn't work... try this one: http://www.jbmronline.com/doi/full/10.1359/jbmr.2000.15.8.1477 Drew On Tue, Dec 8, 2009 at 08:18, Drew Meyer <41dmb41@gmail.com> wrote: > Is this it? > > http://www.jbmronline.org/doi/full/10.1359/jbmr.2000.15.8.1477?cookieSet=1 > > I've never read the paper, so I don't know exactly what you're looking > for... but I sometimes find Google Scholar to be a better search > engine for journal articles (which is what I used to find the above > article). > > Drew > > On Tue, Dec 8, 2009 at 01:36, louise renton wrote: >> Hi all, >> >> I am going crazy - and its not even friday yet! A little while ago (OK about >> 3 years) i came across a paper where the researcher had shown the transition >> of macrophages to osteoclasts ?using immuno staining - i cannot seem to find >> it on Pubmed, dont know the author date or journal. >> >> i hope soemone out there can help >> >> best egards >> >> -- >> Louise Renton >> Bone Research Unit >> University of the Witwatersrand >> Johannesburg >> South Africa >> "There are nights when the wolves are silent and only the moon howls". >> George Carlin >> No trees were killed in the sending of this message. >> However, many electrons were terribly inconvenienced. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From joost.bruijntjes <@t> tno.nl Tue Dec 8 09:03:21 2009 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Tue Dec 8 09:03:29 2009 Subject: [Histonet] (no subject) Message-ID: <8865601DD17A554CB489C17FFD8A51B203046B4C@MAIL04.tsn.tno.nl> Hi histonetters I was wondering if anyone of you has ever used an antibody against P63 on chicken tissues? I can find a lot of antibodies, but none of these have ever been tested on chicken tissues. If someone has recommendations please feel free to contact me! Thanks a lot. Joost Bruijntjes TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From a_jimmy11 <@t> yahoo.com Tue Dec 8 09:34:13 2009 From: a_jimmy11 <@t> yahoo.com (Jimmy A) Date: Tue Dec 8 09:34:18 2009 Subject: [Histonet] Working on Ventana IHC Stainers Message-ID: <757617.75346.qm@web112815.mail.gq1.yahoo.com> ? ? ?Hi, ???? I am an histotech working in the US. I am interested in learning? the operation of the ventana immunostainers. I will appreciate it, if one of the?histology/ihc labs could?grant me?this great favour. ??? Hoping to hear from you guys asap. ? ????? Jimmy A From cmiller <@t> gladstone.ucsf.edu Tue Dec 8 11:58:59 2009 From: cmiller <@t> gladstone.ucsf.edu (Caroline Miller) Date: Tue Dec 8 11:59:05 2009 Subject: [Histonet] Advice about human autopsy samples in a purely research core lab Message-ID: <830F0D77-7CFA-469D-9287-A7D4D580217E@gladstone.ucsf.edu> Hi Histoland! I work in a small histology and microscopy core (2 members of staff) in San Francisco at the Gladstone Institutes (affiliated with UCSF). We have recently had an enquiry from a private autopsy service (with LLC in their name) about human autopsy samples for processing, cutting, HE staining and maybe a few special stains here and there. My institutes would like to have a contract in place for this work to cover our bases and I would like some advice on what to include in that contract. I have asked about regulations, and they say that their information is private and not something monitored by outside agencies like CAP, JCAHO, because they are not a diagnostic lab dealing with living patients. They say that JCAHO doesn't apply because they are not delivering health care. They also say that: "An autopsy is not a surgical/therapeutic or "lab test" procedure by any states law except Connecticut. " and HIPPA doesn't apply because the patient is deceased. I have worked with human tissue before, I ran a histo lab in London UK so I am well aware of the UK laws, but unfortunately not the US ones. So I ask the histoland: Is this all true? Are there any sticking points we may run into with the regulations? How could we phrase the contract so that there is no chance of come-back? Big questions, I know, but any advice would be gratefully received Yours Caroline Caroline Miller, M.Sc, DIC, AIBMS Manager Histology and Microscopy Core J David Gladstone Institutes 1650 Owens St San Francisco CA 94158 Tel: 415 734 2566 Fax: 415 355 0824 Cell: 415 218 7297 http://www.gladstone.ucsf.edu/gladstone/site/histology/ cmiller@gladstone.ucsf.edu From alonso.martinezcanabal <@t> utoronto.ca Tue Dec 8 12:41:16 2009 From: alonso.martinezcanabal <@t> utoronto.ca (alonso.martinezcanabal@utoronto.ca) Date: Tue Dec 8 12:41:21 2009 Subject: [Histonet] TSA Message-ID: <20091208134116.0cstoxpbksgos4gg@webmail.utoronto.ca> Hi, We are working here with TSA amplification kits, using them in free floating sections or mounted sections. Normally our sections are thick, like 40um. We consistently have a lack of an even distribution of the staining, normally staying in the first couple of microns if the antigen is very abundant (like NeuN) or being more even with very sparse cells. Does anyone know what couls be happening here, how could we solve this? thank you very much. By the way, anyone knows another way to aplify fluorescence signal that does not involve TSA, like ABC kit... Thank you very much From a_jimmy11 <@t> yahoo.com Tue Dec 8 12:55:43 2009 From: a_jimmy11 <@t> yahoo.com (Jimmy A) Date: Tue Dec 8 12:55:47 2009 Subject: [Histonet] Ventana Stainer Message-ID: <972367.10090.qm@web112812.mail.gq1.yahoo.com> ? ? ?? ????? Hi ? ? I am looking for a lab that can allow me to spend one or two days to properly see how the ventana autostainer?works. ???? I am an histotech working here?in the US. I am interested in learning? the operation of the ventana immunostainers. I will appreciate it, if one of the?histology/ihc labs could?grant me?this great favour. ???? ?Hoping to hear from you guys asap. ? ???? Jimmy. From Bauer.Karen <@t> mayo.edu Tue Dec 8 15:22:18 2009 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Tue Dec 8 15:22:27 2009 Subject: [Histonet] Linear Slide Stainers Message-ID: <53FC421CC200C5429929EDE6C3676F30A19834@msgebe34> Hello... I've been told that the GLX and SLS Linistat linear slide stainers are being discontinued. I was going to order a GLX for our new Mohs lab, and now I'm unsure what to purchase instead. What are others using? Do you need a fume hood? If I go formalin free and xylene free, do I need a hood? I appreciate any suggestions you may have. Thanks much, Karen Karen L. Bauer HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System bauer.karen@mayo.edu From ndevans <@t> stanford.edu Tue Dec 8 15:57:07 2009 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Tue Dec 8 15:57:10 2009 Subject: [HISTONET] fixation and storage of tissue for TEM Message-ID: <583B381A50D446848EDA91C4AC322712@DellDesktop2> Dear all, I would like to fix and preserve mouse skin tissue for processing for transmission electron microscopy (TEM) at a later date. I was wondering whether I can store tissue following fixation overnight in 2.5% gluteraldehyde in 0.1M sodium cacodylate buffer and, if so, whether it can be stored indefinitely in this buffer (or whether I need to treat with OsO4 and store at 70% ethanol)? Thanks and best wishes Nick From mwich <@t> 7thwavelabs.com Wed Dec 9 10:11:21 2009 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Wed Dec 9 10:13:16 2009 Subject: [Histonet] solution to removing coverglass on GMA sections Message-ID: <62A8156F8071C8439080D626DF8C33A65D8EA8@wave-mail.7thwave.local> I realize that I am a bit delayed in expressing my gratitude, but thanks for all the responses to my inquiry about removing coverglass from slides containing GMA sections mounted with "UV mount." I wanted to share the procedure that did finally work incase anyone comes across this issue: Simply soak the slides in a 50:50 mixture of JB-4 Solution A (Monomer) and acetone for a full work day (or overnight), and rinse in acetone. Thanks again. Histonet is a beautiful thing. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From leiker <@t> buffalo.edu Wed Dec 9 10:28:48 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Dec 9 10:29:00 2009 Subject: SPAM-LOW: Reponses to Merced and Richard Re: [Histonet] cryojane tape transfer system In-Reply-To: <057346ACEE754D0098ECB09C65412359@prueggihctechlt> References: <000101ca6c61$541e4d60$fc5ae820$@callis@bresnan.net> <057346ACEE754D0098ECB09C65412359@prueggihctechlt> Message-ID: <12D66B9C5986437A41CC49B5@CDYwxp1931.ad.med.buffalo.edu> I guess I did not realize what ongoing discussion my comment about the CryoJane tape transfer would spark! Thanks to the replies I have received in private and in public, I see that it indeed helps one make lovely bone sections. In the past 9 years that I have cryosectioned, I have done a range of soft tissues, but never bone. CryoJane had been pushed on me a time or two by a rep to use on my (non-bone) tissues. I discovered, however, that it was not necessary on my (non-bone) tissues. I was never informed of using it on bone (until now!), never even crossed my mind, since I'd never done bone. So, I'm sorry for anyone who may have been offended by my question about the use of the CryoJane tape transfer. I indeed figured I had to be missing something! :-) Regards, Merced --On Sunday, December 06, 2009 2:31 PM -0700 Patsy Ruegg wrote: > Here, here Gayle, I have a picture on my website of a calcified horse > carpal bone I cut using the Instrumedics tape transfer system, I bet no > one would have been able to do that without using the tape. > www.ihctech.net > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle > callis Sent: Monday, November 23, 2009 10:21 AM > To: 'Histonet' > Cc: emmanuel.mineo@leica-microsystems.com > Subject: SPAM-LOW: Reponses to Merced and Richard Re: [Histonet] cryojane > tape transfer system > > You wrote: > > > > Unless I'm missing something, I don't understand why people use this > tape? > > It seems like a marketing gimmick to me...ol' fashion' melting of > sections > > onto slides works perfectly for us... > > ? > > Regards, > > Merced > > > > Merced, > > Yes, you are missing something. If you have ever tried to cryosection > undecalcified bone or extremely difficult tissues that simply will not > result in "ol' fashion' melting" onto a slide , then you would understand > why people use this unique cryosectioning system. It is not some > "marketing gimmick" but an unique instrument helping many laboratories > obtain frozen sections that otherwise are scrunched up, shattered, and > destroyed. I suggest you go to the Instrumedics website or > www.alphelys.com for a superb slide show and learn how this instrument > works before making assumptions about a technology that serves many of us > more than well. > > > > A happy, informed user of the Cryojane................ > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > Bozeman MT > > > > As for what Richard wrote: > > > > Hi Everybody, > I was hoping to get some advice - I'm cryosectioning plant tissues and > transferring sections to slides using the Cryojane system. However, i'm > having problems in transferring the sections without them falling apart > during the tape transfer. I'm fixing my tissue for 24 hours in > ethanol:acetic acid (3:1), embedding in O.C.T, snap-freezing and then > sectioning at between 2 and 14 microns. The sections seem to be ok but > whenever i remove the adhesive tape from the slide a large part of the > tissue is removed with it. As a result I lose the majority of my > section. I've tried using both 1x and 1/2x slides (CFSA adhesive slides > from instrumedics) but neither have given satisfactory results. > Does anyone have any suggestions as to how i could reduce the loss of > tissue? Any advice would be much appreciated. > Thanks > Richard > > Dear Richard, > > I could be the fixative you are using that causes the problem. If the > fixative contains alcohol, the alcohol acts as antifreeze when you try to > snap freeze a tissue, animal or plant. The alcohol may cause problems > with how the pink tape sticks to the face of the plant tissue, and allows > them to fall apart during the tape transfer. If you rinse away the > fixative, then you should cryoprotect the fixed plant tissue with 30% > sucrose before snap freezing. vbThis will remove the alcohol. If > cryoprotection causes problems with the final staining results, then try > unfixed plant tissue, snap freeze, Cryojane tape transfer the section and > then fix the transferred plant section in your favorite fixative. You > may have to optimize the time in fixative though. > > Other suggestions: > > Do a double UV flash, but wait for 15 to 20 seconds between flashes. You > must allow the UV light source (capacitor) build up enough charge to work > properly. This double flash seems to help polymerize the coating more > thoroughly, and the section should transfer better. Also, the tape must > be removed at an angle across the slide, very slowly, and inside the > cryostat (I am sure you probably do this already.) > > Also, try the 4X slide if you still have problems with 1/2X and 1X > slides. You might ask Leica to send you a few to try before investing in > a whole box of these. Contact Emmanuel Mineo, Intrumedics Product Manager > emmanuel.mineo@leica-microsystems.com for the 4X slides. Manny is a nice > gentleman who has worked with Cryojane for many years and has always been > helpful to us. Once again, do the double UV light flash with the 4X > slides. They are gooey, but may/should hold more securely. > > With undecalcified bone, we use the 1/2X but do the double flash routinely > for all sections. > > Good luck > > Gayle Callis > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From jkiernan <@t> uwo.ca Wed Dec 9 11:10:17 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Dec 9 11:10:24 2009 Subject: [Histonet] Overstaining - Mayers H&E Message-ID: This is a typical example of the informal "protocols" that get passed on from generation to generation of graduate students, postdocs and technicians in research labs at universities. The original was probably written by someone who knew how to do H&E staining, but on differently fixed tissues, and certainly on thinner sections. It apears to be for individual slides, because 10 seconds in each of the two 95% and 100% ethanols would be effective only with vigorous agitation in a large excess of fluid. The tissue is almost unfixed, unless "Soak in 0.4% paraformaldehyde" means leave it ovenight or longer in 4% formaldehyde. Researchers otherwise educated to the highest levels in such difficult disciplines as molecular biology and neuroscience regularly write phrases like "4% paraformaldehyde", thereby advertising their profound ignorance about fixation, which is the procedure that has the greatest effect on the appearance of anything dead that's examined with a microscope, especially if stains or histochemical methods are to be used. (I apologise for the length of the preceding sentence, but not for its punctuation, which is correct in British but not in American English usage. Check it out with Lynne Truss!) The "sucrose cycle" step, with no times or instructions about floating and sinking, is probably local jargon from a lab where small animals' brains are minimally fixed and cryoprotected before cutting thick (50-100um) frozen sections, to be stained free-floating. That's not an H&E job! You are working with a thin skeletal muscle (rat's gastrocnemius). If your Mayer's haemalum is a bought solution, it is intended for use in hospital labs, on paraffin sections about 5um thick. In a research setting you may need to make changes. Haemalum (Mayer's or anyone else's, correctly used) should stain cell nuclei blue and very little else. An important part of H&E staining is looking at the wet section with a microscope to check for adequate and selective nuclear coloration. In skeletal muscle the nuclei are small, so the haemalum-stained section is very pale blue to the unaided eye. With alcoholic eosin (as in your method) it's not so easy to control the staining with microscopic control, but it will probably be OK if the section is light pink. Some people like their eosin darker; it's largely a matter of taste unless you need to distinguish between different eosinophilic components on the basis of hue. John Kiernan Anatomy, UWO London, Canada = = = -----Original Message ----- From: Josephine Garcia Date: Monday, December 7, 2009 11:43 Subject: [Histonet] Overstaining - Mayers H&E To: histonet@lists.utsouthwestern.edu > Hi all, > > My (frozen-section, fixed) slides are coming out much too dark > (overstainedpurple) and I'm not sure why. They are 15-20 > micrometer slices of rat > gastrocnemius muscle. Can someone please look over our current > protocol and > tell me what I'm doing wrong? Thanks! Here it is: > > 1. Perfuse animal with 4% paraformaldehyde fixative. > 2. Soak in 0.4% paraformaldehyde > 3. Sucrose cycle (5% rinse, 10%, 20%, 30% soak) > 4. Embed in OCT, Frozen sections (15-20 micrometers) > 5. Let dry for 15-30 min > 6. Stain as follows: > > - Distilled H2O (quick dip) > - Mayer's Hematoxylin - 1min (originally we were dipping for 5- > 10 minutes. I > slowly reduced the time to 2min, then 1min, then 30s... still > overstained!)- Running lukewarm tap water - drain and > continuously fill - 15min or until > water runs clear > - Distilled H2O (quick dip) > - 80% EtOH - 1-2min > - Eosin - 2 min > - 95% EtOH I - 10sec > - 95% EtOH II - 10sec > - 100% EtOH I - 10sec > - 100% EtOH II - 10sec > - Xylene I - 2min > - Xylene II - 2min > > 7. Coverslip and let dry overnight > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Dec 9 11:38:27 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Dec 9 11:38:36 2009 Subject: [HISTONET] fixation and storage of tissue for TEM Message-ID: Overnight in glutaraldehyde is more than adequate for the tiny (<1mm) bits of tissue that are needed for transmission electron microscopy. Two hours is often sufficient. The fixation, washing, postosmication, propylene oxide (if used) and preliminary infiltration with catalyzed epoxy resin can then be done within one working day. Polymerization in a capsule at 60C is then an overnight step. If glutaraldehyde-fixed specimens must be held for more than an hour or two, it should be in buffer or water. At least one animal tissue (CNS) does not respond to changes in osmotic pressure after glutaraldehyde fixation (Paljarvi, Garcia & Kalimo 1979 The efficiency of aldehyde fixation for electron microscopy: stabilization of rat brain tissue to withstand osmotic stress. Histochemical Journal 11:267-276). I hope some histonettters will reply to the group with information less than 30 years old about liquid storage of other fixed tissues for later TEM. There are some very good books that provide instructions for preparing tissues for electron microscopy. I highly recommend an inexpensive paperback: Hunter, EE (1993). Practical Electron Microscopy. A Beginner's Illustrated Guide, 2nd ed. Cambridge: Cambridge University Press. ($30 new; $7 second-hand, on Amazon.com.) Some histotechnology textbooks have brief but good good EM chapters. An example is: Brown GG (1978). An Introduction to Histotechnology. New York: Appleton-Century-Crofts. (On Amazon.com this book is priced over $200 but ordinary second-hand bookshops have it for $5 to $10!) Every lab that does TEM should have access to a library that contains the big books in this field: notably the several volumes edited by Glauert and Hayat. The preparative techniques for TEM were understood and perfected before 1970, so it isn't necessary to have the latest editions. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicholas David Evans Date: Tuesday, December 8, 2009 16:58 Subject: [HISTONET] fixation and storage of tissue for TEM To: histonet@lists.utsouthwestern.edu > Dear all, > > > > I would like to fix and preserve mouse skin tissue for > processing for > transmission electron microscopy (TEM) at a later date. I was > wonderingwhether I can store tissue following fixation overnight > in 2.5% > gluteraldehyde in 0.1M sodium cacodylate buffer and, if so, > whether it can > be stored indefinitely in this buffer (or whether I need to > treat with > OsO4 and store at 70% ethanol)? > > > > Thanks and best wishes > > Nick > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Wed Dec 9 11:40:19 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Wed Dec 9 11:40:24 2009 Subject: [Histonet] Ventana Stainer In-Reply-To: <972367.10090.qm@web112812.mail.gq1.yahoo.com> References: <972367.10090.qm@web112812.mail.gq1.yahoo.com> Message-ID: It probably would help your possibility of success Jimmy if you would tell us where you live so that responses can come from individuals in your area. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jimmy A Sent: Tuesday, December 08, 2009 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Stainer ? ? ?? ????? Hi ? ? I am looking for a lab that can allow me to spend one or two days to properly see how the ventana autostainer?works. ???? I am an histotech working here?in the US. I am interested in learning? the operation of the ventana immunostainers. I will appreciate it, if one of the?histology/ihc labs could?grant me?this great favour. ???? ?Hoping to hear from you guys asap. ? ???? Jimmy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Dec 9 11:48:38 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Dec 9 11:48:43 2009 Subject: [Histonet] paper on osteoclasts In-Reply-To: References: Message-ID: It's probably not the same paper, but may help: Blumer MJF, Longato S, Fritsch H (2008) Localization of tartrate-resistant acid phosphatase (TRAP), membrane type-1 matrix metalloproteinases (MT1-MPP) and macrophages during early endochondral bone formation. J. Anat. 213: 431-441. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: louise renton Date: Tuesday, December 8, 2009 1:37 Subject: [Histonet] paper on osteoclasts To: Histonet@lists.utsouthwestern.edu > Hi all, > > I am going crazy - and its not even friday yet! A little while > ago (OK about > 3 years) i came across a paper where the researcher had shown > the transition > of macrophages to osteoclasts using immuno staining - i > cannot seem to find > it on Pubmed, dont know the author date or journal. > > i hope soemone out there can help > > best egards > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Weber2 <@t> va.gov Wed Dec 9 11:45:49 2009 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Wed Dec 9 11:51:10 2009 Subject: [Histonet] unsubscribe please Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB9098BBA22@VHAV10MSGA1.v10.med.va.gov> Susan Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VAMC (216) 791-3800 x6154 From litepath2000 <@t> yahoo.com Wed Dec 9 12:31:18 2009 From: litepath2000 <@t> yahoo.com (NYSHisto) Date: Wed Dec 9 12:31:28 2009 Subject: [Histonet] RE: bachelor's degrees and NYS Licensure Message-ID: <497242.5369.qm@web58805.mail.re1.yahoo.com> Dear Nate & All First, let me begin by saying that I more than understand your (and everyone?s) frustration regarding the difficulty and often distressing path to obtain a license in NYS.? Second, let me clearly state that my intention in this email is not to argue the pros or cons of licensure or to endorse or refute a particular point of view. The truth is that licensure is a requirement in NYS and we must adapt to this reality. The goal is to (briefly) explain, good or bad, what has transpired in NYS over the last few years so that everyone can understand and hopefully prevent similar situations from developing in their respective states.? From a historical perspective, the ?path? taken to get to this point has had many twists, turns and some serious potholes. The process spans more than 15 years of negotiations and deliberations by political, academic, corporate, union and hospital entities as well as educators, histologists, pathologists, activists and lawyers. It is beyond the scope of this email to try and summarize everything that has taken place in that time frame.? ?More recently (preceding the passage of the law), labor unions and national organizations wrestled over the language and structure of the law with one side strongly opposed and the other solidly in favor of licensing. These organizations interests were just that, their interests based on furthering their agendas, not those of the technician, technologist or profession. Under these organizations influence, legislation was passed, the law was implemented and the regulations created. It is important to note that the law, as it was originally written, did not mention histologist or the histology discipline. In fact, the educational requirements to practice in the medical laboratory field (any sub-discipline) required a bachelors degree with a curriculum derived almost completely from the clinical side of the laboratory. The only anatomic pathology component was a semester of microscopic histology. In essence, the initial law grouped all sub-disciplines together (from blood-banking to histology) and created a set of educational requirements that, in the eyes of the NYS education department, established an individual?s qualifications to work in the medical laboratory profession in NYS. ?It should also be noted that early in this process (to the best of my knowledge) it was proposed/recommended that the law be structured similarly to the classification system already established by the ASCP BOR for certifying medical laboratory professionals.? This idea was also suggested recently but was rejected by the major players at the negotiating table.? Interestingly, this approach would have been far better for fattening the state coffers. I would fully agree with Ren?. The spirit of the law is intended to ensure that individuals who have an education are not undermined by those that are placed into laboratories as ?stop-gap? (either for financial or staffing reasons) measures by facility administrations potentially compromising patient care. ?We believe that this has principally arisen out of the increasing medical laboratory personnel shortage which can be in part, attributed to the low salaries plus the poor decision making of administrations determined to sacrifice ?quality for quantity?.? ?Unfortunately, as is more often the case, the spirit of the law is lost in the wording. Much of this transpired out of site of the average working tech and therein lays one of the major problems.? Be it complacency, lack of involvement, or because of our busy schedules we allowed others to make decisions that directly impacted us and our profession. ??By the time we realized what was happening, much of the damage had already been done. Nevertheless, the NYS Histotechnological Society immediately mobilized and began lobbying to revise the law so that it better represented the interests of current and future histology professionals in NYS. We have continued to maintain a presence on the state legislative level as well as communicate with the NYS education department (Board of clinical Laboratory Technology, Office of Professions). In regards to the examination, the only public comments that I am aware that the state initially made was that it was in the ?process of seeking an organization to administer the exam?.? Almost all parties recommended that the state use the ASCP BOR (now called the ASCP Board of Certification) as the model and for the actual examination. To the best of my knowledge, NSH was not involved in the decision cycle.? Again, it is important to keep in mind that there is a significant difference between the state licensing examination and the ASCP board certification. The NYS examination(s), which most likely will be administered by the BOC, is only intended to demonstrate that minimum competency (based on the NYS regulations) has been met.? In contrast, the ASCP board certification is intended to demonstrate proficiency and expertise. In February of this year, the ASCP BOC was awarded a contract by the NYS Education Department to be the sole provider of licensure examinations for medical technicians and technologist (NYS clinical laboratory technicians and technologist).? It seems likely, although not certain, that the HT examination will be appended to this contract.? As mentioned above, the initial curricular criteria for examination eligibility was unrealistic for histologist, since it was based entirely on a curriculum for a medical laboratory generalist, principally a bachelor?s degree in clinical pathology.? During negotiations for an amendment to the law (to correct many separate flaws), we proposed that histology be considered separately from the other sub-disciplines, analogous to the differentiation that is already made by pathologist, i.e.:?CP and AP?. Once again, the goal was to establish a minimum set of curriculum requirements for individuals to practice histology in the field. Those educational criteria were based on the educational curriculum at SUNY Cobleskill since this was the only histotechnology program in NYS at the time.? In fact, without this amendment, the program would have been forced to close and would have eliminated any dedicated histotechnology programs in NYS.? Regardless, the NYS Education Department acknowledged that there was a significant difference between the education of a histologist and medical laboratory technician/technologist and that those differences warranted a different set of educational criteria to establish competency. Furthermore and to clarify, an individual does not need to have the Associate?s Degree in order to practice histology in NYS.? Individuals with Bachelor?s degrees that meet all of the states requirements (education, practical and examination) for licensing can practice histotechnology in NYS. ?As a result of the amendment the program at SUNY Cobleskill is open and began accepting students this year.? The shortage has also prompted several other colleges to explore and commit to creating campus based and distance learning programs.? We hope that these programs will ?bear fruit? but given the economic developments over the past few years and the budgetary constraints on the educational system, the outcome is uncertain. Finally, we should not fault the bureaucrats for something that has been happening in our ?own backyard? for several years.?? We can all agree that the shortage is only going to get worse. ?Perhaps the bureaucrats viewed licensure as a preemptive measure to ensure that patient care was not compromised, especially considering the complexity that is health care reform. Regardless, it is up to all of us to work together to help correct issues/flaws by being active and aware of what is happening at the hospital, local, state and national levels. No one will do this for us, it is our responsibility. ?At any rate, I hope that this helps clarify some aspects of the convoluted path to licensure in NYS. ?If anyone has any questions please do not hesitate to contact us. ? Respectfully Luis Chiriboga & Amy Farnan,? NYSHS Legislative Committee ?------- Luis Chiriboga Ph.D. Assistant Professor of Pathology Vice President New York State Histotechnological Society NYSHS Website www.nyhisto.org NYSHS Message Board http://tech.groups.yahoo.com/group/NYSHS1972/ From foreightl <@t> gmail.com Wed Dec 9 12:33:41 2009 From: foreightl <@t> gmail.com (Pat Laurie) Date: Wed Dec 9 12:33:45 2009 Subject: [Histonet] Cutting GI biopsies with multiple levels Message-ID: Histonet, One of our pathologists is looking at a method for cutting GI biopsies that differs in complexity from the way we currently do them. Currently we cut just 2 levels at 4 microns thick, 40 microns apart and only showing 2 sections on each level. In the new plan, if there were a maximum of 5 small pieces per block, the idea is that we would cut 10 serial sections at 4 microns thickness, split the ribbons in half on the waterbath, and then put 5 sections vertically on 1 side of the slide and the other 5 sections vertically on the other side of the slide. We would then face in 40 microns and repeat the process for a second slide for a total of 2 slides, 20 sections of tissue, 120 microns between the first and the last section. I am happy to do this if our pathologists believe that it would improve their diagnosis. Since we do about 400 biopsies like this a month, I would like to look at our workload to see how to change our staffing with this method. I would like to get an idea of the difference in time between the 2 methods. Does anyone else do a similar method and would be willing to share their experiences? I am primarily interested in the amount of time it would take an experienced tech to cut a biopsy like this. We have tried it out, but our techs are far from experienced with this method. Thanks for your time. -- Patrick Laurie HT(ASCP)QIHC Histology Supervisor CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com From kc <@t> ka-recruiting.com Wed Dec 9 12:45:56 2009 From: kc <@t> ka-recruiting.com (K.C. Carpenter) Date: Wed Dec 9 12:45:51 2009 Subject: [Histonet] Histology Jobs Message-ID: <1612807051.1260384356922.JavaMail.cfservice@webserver60> Dear Histonet Subscribers, I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have clients across the country. We are completely free of charge to candidates and are currently working on numerous Histology positions. Our clients often assist with relocation expenses. Below is a list of some of the Histology opportunities we are currently working on: 1) New York City - Histotechnologist -3rd shift 2) New York City - Histology Supervisor - 1st Shift 3) Oklahoma - Histotechnologist - 1st shift 4) Georgia (SE part of the state) - Histology Supervisor - 1st shift 5) Georgia (SE part of the state) - Histotechnologist - 2 positions open 6) California (Bay Area) - Pathology Manager - 1st shift 7) California (Los Angeles) - Histology Supervisor - 1st shift 8) California (San Diego) - Sr. Histotechnologist - 1st shift 9) Massachusetts (Boston) - Histotechnologist - 1st shift Contact me if you're interested in learning more about any of these opportunities or if you'd like like me to tailor a specific search for you. We work on positions at all levels and cover the entire US. Please also send me an updated resume and let me know what the best way to reach you is. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 kc@ka-recruiting.com www.ka-recruiting.com From JWeems <@t> sjha.org Wed Dec 9 12:55:21 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Dec 9 12:55:26 2009 Subject: [Histonet] Cutting GI biopsies with multiple levels In-Reply-To: References: Message-ID: It takes a while to get experienced, but it doubles, sometimes triples the time. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Laurie Sent: Wednesday, December 09, 2009 13:34 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting GI biopsies with multiple levels Histonet, One of our pathologists is looking at a method for cutting GI biopsies that differs in complexity from the way we currently do them. Currently we cut just 2 levels at 4 microns thick, 40 microns apart and only showing 2 sections on each level. In the new plan, if there were a maximum of 5 small pieces per block, the idea is that we would cut 10 serial sections at 4 microns thickness, split the ribbons in half on the waterbath, and then put 5 sections vertically on 1 side of the slide and the other 5 sections vertically on the other side of the slide. We would then face in 40 microns and repeat the process for a second slide for a total of 2 slides, 20 sections of tissue, 120 microns between the first and the last section. I am happy to do this if our pathologists believe that it would improve their diagnosis. Since we do about 400 biopsies like this a month, I would like to look at our workload to see how to change our staffing with this method. I would like to get an idea of the difference in time between the 2 methods. Does anyone else do a similar method and would be willing to share their experiences? I am primarily interested in the amount of time it would take an experienced tech to cut a biopsy like this. We have tried it out, but our techs are far from experienced with this method. Thanks for your time. -- Patrick Laurie HT(ASCP)QIHC Histology Supervisor CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From mcauliff <@t> umdnj.edu Wed Dec 9 13:08:16 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Dec 9 13:06:18 2009 Subject: [Histonet] was Overstaining - Mayers H&E In-Reply-To: References: Message-ID: <4B1FF5A0.6060005@umdnj.edu> On the subject of protocols being passed down from person to person, lab to lab, etc., a freshly minted PhD came to me once looking for some NaH with which to make buffer. I explained that there was no such chemical as NaH but she insisted: "here is the protocol." A simple typographical error had left out the O in NaOH. Because she had a full time tech at her beck and call throughout her PhD training she had never learned how to make simple solutions. Sad but true. Geoff John Kiernan wrote: > This is a typical example of the informal "protocols" that get passed on from generation to generation of graduate students, postdocs and technicians in research labs at universities. The original was probably written by someone who knew how to do H&E staining, but on differently fixed tissues, and certainly on thinner sections. It apears to be for individual slides, because 10 seconds in each of the two 95% and 100% ethanols would be effective only with vigorous agitation in a large excess of fluid. > > The tissue is almost unfixed, unless "Soak in 0.4% paraformaldehyde" means leave it ovenight or longer in 4% formaldehyde. > Researchers otherwise educated to the highest levels in such difficult disciplines as molecular biology and neuroscience regularly write phrases like "4% paraformaldehyde", thereby advertising their profound ignorance about fixation, which is the procedure that has the greatest effect on the appearance of anything dead that's examined with a microscope, especially if stains or histochemical methods are to be used. (I apologise for the length of the preceding sentence, but not for its punctuation, which is correct in British but not in American English usage. Check it out with Lynne Truss!) > > The "sucrose cycle" step, with no times or instructions about floating and sinking, is probably local jargon from a lab where small animals' brains are minimally fixed and cryoprotected before cutting thick (50-100um) frozen sections, to be stained free-floating. That's not an H&E job! You are working with a thin skeletal muscle (rat's gastrocnemius). > > If your Mayer's haemalum is a bought solution, it is intended for use in hospital labs, on paraffin sections about 5um thick. In a research setting you may need to make changes. Haemalum (Mayer's or anyone else's, correctly used) should stain cell nuclei blue and very little else. > > An important part of H&E staining is looking at the wet section with a microscope to check for adequate and selective nuclear coloration. In skeletal muscle the nuclei are small, so the haemalum-stained section is very pale blue to the unaided eye. With alcoholic eosin (as in your method) it's not so easy to control the staining with microscopic control, but it will probably be OK if the section is light pink. Some people like their eosin darker; it's largely a matter of taste unless you need to distinguish between different eosinophilic components on the basis of hue. > > John Kiernan > Anatomy, UWO > London, Canada > = = = > -----Original Message ----- > From: Josephine Garcia > Date: Monday, December 7, 2009 11:43 > Subject: [Histonet] Overstaining - Mayers H&E > To: histonet@lists.utsouthwestern.edu > > >> Hi all, >> >> My (frozen-section, fixed) slides are coming out much too dark >> (overstainedpurple) and I'm not sure why. They are 15-20 >> micrometer slices of rat >> gastrocnemius muscle. Can someone please look over our current >> protocol and >> tell me what I'm doing wrong? Thanks! Here it is: >> >> 1. Perfuse animal with 4% paraformaldehyde fixative. >> 2. Soak in 0.4% paraformaldehyde >> 3. Sucrose cycle (5% rinse, 10%, 20%, 30% soak) >> 4. Embed in OCT, Frozen sections (15-20 micrometers) >> 5. Let dry for 15-30 min >> 6. Stain as follows: >> >> - Distilled H2O (quick dip) >> - Mayer's Hematoxylin - 1min (originally we were dipping for 5- >> 10 minutes. I >> slowly reduced the time to 2min, then 1min, then 30s... still >> overstained!)- Running lukewarm tap water - drain and >> continuously fill - 15min or until >> water runs clear >> - Distilled H2O (quick dip) >> - 80% EtOH - 1-2min >> - Eosin - 2 min >> - 95% EtOH I - 10sec >> - 95% EtOH II - 10sec >> - 100% EtOH I - 10sec >> - 100% EtOH II - 10sec >> - Xylene I - 2min >> - Xylene II - 2min >> >> 7. Coverslip and let dry overnight >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mcauliff <@t> umdnj.edu Wed Dec 9 13:15:57 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Dec 9 13:13:59 2009 Subject: [HISTONET] fixation and storage of tissue for TEM In-Reply-To: <583B381A50D446848EDA91C4AC322712@DellDesktop2> References: <583B381A50D446848EDA91C4AC322712@DellDesktop2> Message-ID: <4B1FF76D.70505@umdnj.edu> Fix in buffered glutaraldehyde for 2 hours, overnight is acceptable. After multiple rinses, store in buffer in the refrigerator. Change the buffer once a week, there are things that will grow in cacodylate buffer. You may complete osmication if you want, then rinse and store in buffer. I would not store the tissue indefinitely in anything. Do NOT store the tissue in 70% ethanol, either before or after osmium, ethanol has been shown to extract cytoplasm from fixed tissues. The texts John Kiernan recommends are excellent. Geoff Nicholas David Evans wrote: > Dear all, > > > > I would like to fix and preserve mouse skin tissue for processing for > transmission electron microscopy (TEM) at a later date. I was wondering > whether I can store tissue following fixation overnight in 2.5% > gluteraldehyde in 0.1M sodium cacodylate buffer and, if so, whether it can > be stored indefinitely in this buffer (or whether I need to treat with > OsO4 and store at 70% ethanol)? > > > > Thanks and best wishes > > Nick > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From batesf <@t> ohsu.edu Wed Dec 9 13:18:21 2009 From: batesf <@t> ohsu.edu (Florence Leomiti) Date: Wed Dec 9 13:18:27 2009 Subject: [Histonet] was Overstaining - Mayers H&E In-Reply-To: <4B1FF5A0.6060005@umdnj.edu> References: <4B1FF5A0.6060005@umdnj.edu> Message-ID: <311B5F326A1C0E4D8CACC6F278FCACEA0351DB52CB@EX-MB07.ohsu.edu> Looking at your protocol... it seems like you are letting it sit in tap water too long... that is where you will get the over stain of the hematoxylin try just a quick wash with tap water and continue on with the rest of the protocol. Florence Leomiti HT (ASCP) Neuromuscular Lab Tech. Phone 503-494-6781 Fax 503-494-6787 Pager 16822 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Wednesday, December 09, 2009 11:08 AM To: John Kiernan Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] was Overstaining - Mayers H&E On the subject of protocols being passed down from person to person, lab to lab, etc., a freshly minted PhD came to me once looking for some NaH with which to make buffer. I explained that there was no such chemical as NaH but she insisted: "here is the protocol." A simple typographical error had left out the O in NaOH. Because she had a full time tech at her beck and call throughout her PhD training she had never learned how to make simple solutions. Sad but true. Geoff John Kiernan wrote: > This is a typical example of the informal "protocols" that get passed on from generation to generation of graduate students, postdocs and technicians in research labs at universities. The original was probably written by someone who knew how to do H&E staining, but on differently fixed tissues, and certainly on thinner sections. It apears to be for individual slides, because 10 seconds in each of the two 95% and 100% ethanols would be effective only with vigorous agitation in a large excess of fluid. > > The tissue is almost unfixed, unless "Soak in 0.4% paraformaldehyde" means leave it ovenight or longer in 4% formaldehyde. > Researchers otherwise educated to the highest levels in such difficult disciplines as molecular biology and neuroscience regularly write phrases like "4% paraformaldehyde", thereby advertising their profound ignorance about fixation, which is the procedure that has the greatest effect on the appearance of anything dead that's examined with a microscope, especially if stains or histochemical methods are to be used. (I apologise for the length of the preceding sentence, but not for its punctuation, which is correct in British but not in American English usage. Check it out with Lynne Truss!) > > The "sucrose cycle" step, with no times or instructions about floating and sinking, is probably local jargon from a lab where small animals' brains are minimally fixed and cryoprotected before cutting thick (50-100um) frozen sections, to be stained free-floating. That's not an H&E job! You are working with a thin skeletal muscle (rat's gastrocnemius). > > If your Mayer's haemalum is a bought solution, it is intended for use in hospital labs, on paraffin sections about 5um thick. In a research setting you may need to make changes. Haemalum (Mayer's or anyone else's, correctly used) should stain cell nuclei blue and very little else. > > An important part of H&E staining is looking at the wet section with a microscope to check for adequate and selective nuclear coloration. In skeletal muscle the nuclei are small, so the haemalum-stained section is very pale blue to the unaided eye. With alcoholic eosin (as in your method) it's not so easy to control the staining with microscopic control, but it will probably be OK if the section is light pink. Some people like their eosin darker; it's largely a matter of taste unless you need to distinguish between different eosinophilic components on the basis of hue. > > John Kiernan > Anatomy, UWO > London, Canada > = = = > -----Original Message ----- > From: Josephine Garcia > Date: Monday, December 7, 2009 11:43 > Subject: [Histonet] Overstaining - Mayers H&E > To: histonet@lists.utsouthwestern.edu > > >> Hi all, >> >> My (frozen-section, fixed) slides are coming out much too dark >> (overstainedpurple) and I'm not sure why. They are 15-20 >> micrometer slices of rat >> gastrocnemius muscle. Can someone please look over our current >> protocol and >> tell me what I'm doing wrong? Thanks! Here it is: >> >> 1. Perfuse animal with 4% paraformaldehyde fixative. >> 2. Soak in 0.4% paraformaldehyde >> 3. Sucrose cycle (5% rinse, 10%, 20%, 30% soak) >> 4. Embed in OCT, Frozen sections (15-20 micrometers) >> 5. Let dry for 15-30 min >> 6. Stain as follows: >> >> - Distilled H2O (quick dip) >> - Mayer's Hematoxylin - 1min (originally we were dipping for 5- >> 10 minutes. I >> slowly reduced the time to 2min, then 1min, then 30s... still >> overstained!)- Running lukewarm tap water - drain and >> continuously fill - 15min or until >> water runs clear >> - Distilled H2O (quick dip) >> - 80% EtOH - 1-2min >> - Eosin - 2 min >> - 95% EtOH I - 10sec >> - 95% EtOH II - 10sec >> - 100% EtOH I - 10sec >> - 100% EtOH II - 10sec >> - Xylene I - 2min >> - Xylene II - 2min >> >> 7. Coverslip and let dry overnight >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ndevans <@t> stanford.edu Wed Dec 9 13:40:47 2009 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Wed Dec 9 13:40:50 2009 Subject: [HISTONET] fixation and storage of tissue for TEM In-Reply-To: <4B1FF76D.70505@umdnj.edu> References: <583B381A50D446848EDA91C4AC322712@DellDesktop2> <4B1FF76D.70505@umdnj.edu> Message-ID: Thanks for the helpful replies and for the reading suggestions. We are fixing for a collaborator who'll do the embedding, so I just wanted to check on the best way to store/transport. This makes it clearer. Best wishes Nick -----Original Message----- From: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Sent: Wednesday, December 09, 2009 11:16 AM To: Nicholas David Evans Cc: histonet@lists.utsouthwestern.edu Subject: Re: [HISTONET] fixation and storage of tissue for TEM Fix in buffered glutaraldehyde for 2 hours, overnight is acceptable. After multiple rinses, store in buffer in the refrigerator. Change the buffer once a week, there are things that will grow in cacodylate buffer. You may complete osmication if you want, then rinse and store in buffer. I would not store the tissue indefinitely in anything. Do NOT store the tissue in 70% ethanol, either before or after osmium, ethanol has been shown to extract cytoplasm from fixed tissues. The texts John Kiernan recommends are excellent. Geoff Nicholas David Evans wrote: > Dear all, > > > > I would like to fix and preserve mouse skin tissue for processing for > transmission electron microscopy (TEM) at a later date. I was wondering > whether I can store tissue following fixation overnight in 2.5% > gluteraldehyde in 0.1M sodium cacodylate buffer and, if so, whether it can > be stored indefinitely in this buffer (or whether I need to treat with > OsO4 and store at 70% ethanol)? > > > > Thanks and best wishes > > Nick > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From fong <@t> zoology.ubc.ca Wed Dec 9 13:40:50 2009 From: fong <@t> zoology.ubc.ca (Angelina Fong) Date: Wed Dec 9 13:41:04 2009 Subject: [Histonet] Help! Losing sections from Superfrost Plus Slides Message-ID: <4B1FFD42.2040406@zoology.ubc.ca> Hi all, We have suddenly started losing tissue sections from our Superfrost Plus Slides. Our students have been cutting fixed, frozen, cryosections (20um) and thaw-mounting these onto Superfrost Plus slides. We have suddenly started losing lots of sections from these slides (again!!). The tissue is small - ie cross sections of frog aorta and longitudinal sections of nerves, so any lose of adhesion results in total loss of the tissue. The odd thing is that she is not losing every section on every slide, but half to 3/4 of the sections are falling off within the first rinse. The sections are from the same tissue block on the same slide while some falls and others don't. We are at a lose as to what we can do to rescue these sections. Does anyone know if there is any way to coat the slides in some solution with the tissue on them to help improve the adhesion without losing the ability to do immunofluorescence? Any further advice on cutting / drying protocols are welcomed. This keeps happening and the inconsistency of it has us so frustrated with this that we are thinking of going back to subbing our own slides. Thanks for your help! Angelina -- ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o Angelina Y. Fong, Ph.D. Department of Zoology Biological Sciences Building 6270 University Boulevard University of British Columbia Vancouver, BC, V6T 1Z4 Canada Ph: (604) 822-5799 Fax: (604) 822-2416 Email: fong@zoology.ubc.ca From histosearch <@t> gmail.com Wed Dec 9 14:08:57 2009 From: histosearch <@t> gmail.com (HistoLab) Date: Wed Dec 9 14:09:02 2009 Subject: [Histonet] Help! Losing sections from Superfrost Plus Slides Message-ID: <521c6d260912091208n421bf467g3240121cd26f06a0@mail.gmail.com> Angelina, I have seen this post a few times before and sometimes the problem was the drying technique or making sure your water bath is clean and free of any debris. I also remember hearing about someone pre-treating their slides with a Trilogy (EDTA) buffer in a pressure cooker. Good Luck! *Matthew Semovoski* Sales Manager Gorilla Scientific Corporation 1-866-435-4977 www.gorillascientific.com From rjbuesa <@t> yahoo.com Wed Dec 9 14:16:37 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 9 14:16:42 2009 Subject: [Histonet] Cutting GI biopsies with multiple levels In-Reply-To: Message-ID: <925301.13642.qm@web65705.mail.ac4.yahoo.com> An experienced histotach can cut regular blocks at a rate of 24/hour. What you describe will reduce the productivity to 8 to 10 blocks/hour. Ren? J. --- On Wed, 12/9/09, Weems, Joyce wrote: From: Weems, Joyce Subject: RE: [Histonet] Cutting GI biopsies with multiple levels To: "Pat Laurie" , histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 1:55 PM It takes a while to get experienced, but it doubles, sometimes triples the time. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Laurie Sent: Wednesday, December 09, 2009 13:34 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting GI biopsies with multiple levels Histonet, One of our pathologists is looking at a method for cutting GI biopsies that differs in complexity from the way we currently do them.? Currently we cut just 2 levels at 4 microns thick, 40 microns apart and only showing 2 sections on each level.? In the new plan, if there were a maximum of 5 small pieces per block, the idea is that we would cut 10 serial sections at 4 microns thickness, split the ribbons in half on the waterbath, and then put 5 sections vertically on 1 side of the slide and the other 5 sections vertically on the other side of the slide.? We would then face in 40 microns and repeat the process for a second slide for a total of 2 slides, 20 sections of tissue, 120 microns between the first and the last section. I am happy to do this if our pathologists believe that it would improve their diagnosis.? Since we do about 400 biopsies like this a month, I would like to look at our workload to see how to change our staffing with this method.? I would like to get an idea of the difference in time between the 2 methods.? Does anyone else do a similar method and would be willing to share their experiences?? I am primarily interested in the amount of time it would take an experienced tech to cut a biopsy like this.? We have tried it out, but our techs are far from experienced with this method. Thanks for your time. -- Patrick Laurie HT(ASCP)QIHC Histology Supervisor CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plaurie@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Dec 9 14:30:30 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 9 14:30:34 2009 Subject: [Histonet] Help! Losing sections from Superfrost Plus Slides In-Reply-To: <4B1FFD42.2040406@zoology.ubc.ca> Message-ID: <681591.72559.qm@web65706.mail.ac4.yahoo.com> Prepare a 1% aq. sol. of "Elmer's Glue" and coat the slides with it. Oven dry and use the slides as usual. Ren? J. --- On Wed, 12/9/09, Angelina Fong wrote: From: Angelina Fong Subject: [Histonet] Help! Losing sections from Superfrost Plus Slides To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, December 9, 2009, 2:40 PM Hi all, We have suddenly started losing tissue sections from our Superfrost Plus Slides. Our students have been cutting fixed, frozen, cryosections (20um) and thaw-mounting these onto Superfrost Plus slides.? We have suddenly started losing lots of sections from these slides (again!!).? The tissue is small - ie cross sections of frog aorta and longitudinal sections of nerves, so any lose of adhesion results in total loss of the tissue.? The odd thing is that she is not losing every section on every slide, but half to 3/4 of the sections are falling off within the first rinse.? The sections are from the same tissue block on the same slide while some falls and others don't. We are at a lose as to what we can do to rescue these sections. Does anyone know if there is any way to coat the slides in some solution with the tissue on them to help improve the adhesion without losing the ability to do immunofluorescence? Any further advice on cutting / drying protocols are welcomed. This keeps happening and the inconsistency of it has us so frustrated with this that we are thinking of going back to subbing our own slides. Thanks for your help! Angelina -- ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o Angelina Y. Fong, Ph.D. Department of Zoology Biological Sciences Building 6270 University Boulevard University of British Columbia Vancouver, BC, V6T 1Z4 Canada Ph:? (604) 822-5799 Fax: (604) 822-2416 Email: fong@zoology.ubc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christiegowan <@t> msn.com Wed Dec 9 14:37:10 2009 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Wed Dec 9 14:37:14 2009 Subject: [Histonet] Help! Losing sections from Superfrost Plus Slides In-Reply-To: <681591.72559.qm@web65706.mail.ac4.yahoo.com> References: <4B1FFD42.2040406@zoology.ubc.ca>, <681591.72559.qm@web65706.mail.ac4.yahoo.com> Message-ID: I would suggest you check the lot#. You may have received a bad lot and need to get them replaced by the vendor. >> > Hi all, > > We have suddenly started losing tissue sections from our Superfrost Plus Slides. > Our students have been cutting fixed, frozen, cryosections (20um) and thaw-mounting these onto Superfrost Plus slides. We have suddenly started losing lots of sections from these slides (again!!). The tissue is small - ie cross sections of frog aorta and longitudinal sections of nerves, so any lose of adhesion results in total loss of the tissue. The odd thing is that she is not losing every section on every slide, but half to 3/4 of the sections are falling off within the first rinse. The sections are from the same tissue block on the same slide while some falls and others don't. > > We are at a lose as to what we can do to rescue these sections. > > Does anyone know if there is any way to coat the slides in some solution with the tissue on them to help improve the adhesion without losing the ability to do immunofluorescence? > > Any further advice on cutting / drying protocols are welcomed. > This keeps happening and the inconsistency of it has us so frustrated with this that we are thinking of going back to subbing our own slides. > > Thanks for your help! > > Angelina > > > -- ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o > > Angelina Y. Fong, Ph.D. > Department of Zoology > Biological Sciences Building > 6270 University Boulevard > University of British Columbia > Vancouver, BC, V6T 1Z4 > Canada > Ph: (604) 822-5799 > Fax: (604) 822-2416 > Email: fong@zoology.ubc.ca > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology <@t> medsurgpath.com Wed Dec 9 16:07:37 2009 From: histology <@t> medsurgpath.com (histology@medsurgpath.com) Date: Wed Dec 9 16:07:42 2009 Subject: [Histonet] maximum block cutting Message-ID: <52093.68.178.72.125.1260396457.squirrel@webmail.integra.net> Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 From gayle.callis <@t> bresnan.net Wed Dec 9 16:46:25 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Dec 9 16:46:49 2009 Subject: [Histonet] Re: losing prefixed frozen sections from Plus charge slides Message-ID: <000601ca7921$71a3c3c0$54eb4b40$@callis@bresnan.net> You wrote: We have suddenly started losing tissue sections from our Superfrost Plus Slides. Our students have been cutting fixed, frozen, cryosections (20um) and thaw-mounting these onto Superfrost Plus slides. We have suddenly started losing lots of sections from these slides (again!!). The tissue is small - ie cross sections of frog aorta and longitudinal sections of nerves, so any lose of adhesion results in total loss of the tissue. The odd thing is that she is not losing every section on every slide, but half to 3/4 of the sections are falling off within the first rinse. The sections are from the same tissue block on the same slide while some falls and others don't. We are at a loss as to what we can do to rescue these sections. Does anyone know if there is any way to coat the slides in some solution with the tissue on them to help improve the adhesion without losing the ability to do immunofluorescence? Any further advice on cutting / drying protocols are welcomed. This keeps happening and the inconsistency of it has us so frustrated with this that we are thinking of going back to subbing our own slides. Suggestions: 1. It could be a bad lot of slides, however, fixed frozen sections are notorious for falling off slides, even Plus charge. You did not say what fixative you used either? 2. I did not see where you cryoprotect your fixed tissue prior to snap freezing, and this may help, not only to reduce large ice water crystal damage, but also makes sectioning less of a crunchy affair. 3. Dry sections after sectioning in front of a fan at RT, and dry longer. Do NOT store just cut frozens in the cryostat (you didn't say how you handled the section immediately after picking up on the Plus Charge slides. Begin drying at RT immediately after sectioning. 4. Do not touch the surface of slides with fingers, sometime oils from skin transfer to slide surface. 5. It is a waste to money to coat expensive Plus Charge slides and coatings ruin or negate the Plus charge. If you carefully read the slide box insert, you will see this. Do not coat Plus charge slides with anything. If you want to coat slides with a gelatin (protein) subbing solution or Elmers glue (derived from milk products), use regular, clean microscope slides,, coat, air dry and store slides. . 6. Not knowing what or how you rinse, if the rinse is harsh, too fast, it will wash fixed sections off a slide. 7. 20 um is thick, although people do have success with careful and longer drying, depending on the staining method you want to perform. How you thaw this thick section onto a slide may be important. A young man taught us a clever way to do that by mounting section on a cold slide (cryostat chamber temperature, then thawing it an angle for a more gradual melting to surface. (don't put your finger under slide below section so it melts all at once onto the surface). He started at one edge of the section so it thawed from one side to another. He had FLAT sections and no bubbles under a thick fixed section. If your get any unevenness, the section may come up. Picking up a thick section from the blade holder plate may be part of the problem so try his method just for fun and possible success. Dry section well! 8. Fixing the tissue before doing frozen sections compromises the proteins, and once cross linked, tend to not like to stay on Plus charge slides, a common complaint seen on Histonet. Hence, subbing your own slides might be the answer if you had success before. 9. Make sure your blade is sharp so you obtain the flattest section possible. When sectioning, and you did not elaborate - mount first section on slide, lay slide outside cryostat, then pick up the next section next to the first section - we generally put first section closest to label, when picking up, and work down to bottom (away from label) for the rest of the sections if you are performing the mounting from the blade holder plate. Good luck, and be sure to check Histonet Archives for more answers. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT From dcojita <@t> tampabay.rr.com Wed Dec 9 17:38:08 2009 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Wed Dec 9 17:38:13 2009 Subject: [Histonet] arizona state license Message-ID: Does anyone know if Arizona requires a state license for histotechnicians/technologists? From deliadfam <@t> yahoo.com Wed Dec 9 18:03:12 2009 From: deliadfam <@t> yahoo.com (DELIA GARCIA) Date: Wed Dec 9 18:03:16 2009 Subject: [Histonet] arizona state license Message-ID: <412647.2461.qm@smtp121-mob.biz.mail.mud.yahoo.com> No we dont. At least not yet. However certification is strongly preferred around here. -----Original Message----- Date: Wednesday, December 09, 2009 4:39:04 pm To: "'histonet'" From: Subject: [Histonet] arizona state license Does anyone know if Arizona requires a state license for histotechnicians/technologists? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patrickintl <@t> sbcglobal.net Wed Dec 9 20:10:31 2009 From: patrickintl <@t> sbcglobal.net (Thomas Patrick Chuna) Date: Wed Dec 9 20:10:36 2009 Subject: [Histonet] Pathology Opportunity Message-ID: <375864.29016.qm@web82508.mail.mud.yahoo.com> Genitourinary / Urogenital Pathology Opportunity (Relocation Assistance Available) ? Location: Dallas Texas ? Fulltime / Permanent Position ? Generous relocation assistance available! ? Our client is one of healthcare?s leading clinical diagnostics organizations. Grow your career, as they expand their uropath practice. ? Your diagnostic expertise and professionalism will be rewarded in this position. Whether you are an established Genitourinary / Urogenital pathologist, or currently in a fellowship, you owe it to yourself to explore this opportunity. ? Keywords:? Genitourinary, GU Pathology, Urogenital, Uropath ? Overview: ? Provide diagnostics expertise and clinical analysis on specimens.? ? Specifically, this individual will be responsible for diagnosing reproductive and urinary system disorders and diseases utilizing clinical pathological diagnosis.?? ? Requirements: ? Significant experience in Genitourinary / Urogenital pathology is required, and leadership experience is preferred. ? Knowledge of diagnostic equipment and software applications. ? Medical Doctor or equivalent medical professional degree ? Board Certified or eligible in AP or AP/CP by the American Board of Pathology ? 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Thomas Patrick Chuna - Owner Patrick International Specialist In Scientific Information Management Opportunities Email: patrickintl@sbcglobal.net Website: http://www.patrickinternational.net LinkedIn: http://www.linkedin.com/in/patrickinternational From wdesalvo.cac <@t> hotmail.com Wed Dec 9 22:27:13 2009 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Dec 9 22:27:20 2009 Subject: [Histonet] maximum block cutting In-Reply-To: <52093.68.178.72.125.1260396457.squirrel@webmail.integra.net> References: <52093.68.178.72.125.1260396457.squirrel@webmail.integra.net> Message-ID: There are no "industry" or "regulatory" standards that dictate a production maximum for blocks cut or slides produced hourly or daily. It is impossible to compare labs and set productivity standards becauseof the lack of standardized processes and protocols in Histology. I suggest that techs participating in a critical task, such as microtomy, for more than two hours consecutively, cannot consistently perform that task at an optimal productivity rate and error free. We should concenttrate our efforts on standardization of process and error reduction before considering setting production limits. William DeSalvo, B.S., HTL(ASCP) > Date: Wed, 9 Dec 2009 14:07:37 -0800 > From: histology@medsurgpath.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] maximum block cutting > > Hi Histonet, > I know we have discussed the average blocks histotechs cut per hour, but > does anyone know if there is a maximum blocks that can be cut per hour? Or > per day? We have regulations for cytotechs who are reading slides for > maximum slides they can read per hour and are wondering if there is any > similar regulation for histotechs. > Thank you all for you help, > Katelin > > Katelin Lester, HTL (ASCP) > MedSurg Pathology Associates, Inc. > (503) 443-2157 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows Live Hotmail gives you a free,exclusive gift. http://www.microsoft.com/windows/windowslive/hotmail_bl1/hotmail_bl1.aspx?ocid=PID23879::T:WLMTAGL:ON:WL:en-ww:WM_IMHM_7:092009 From starran <@t> hotmail.com Wed Dec 9 22:52:10 2009 From: starran <@t> hotmail.com (sarah tarran) Date: Wed Dec 9 22:52:15 2009 Subject: [Histonet] RE: Help! Losing sections from Superfrost Plus Slides In-Reply-To: References: Message-ID: Hi Angelina, We were having the same problem with frozen mouse aortas. I was cutting at 5ums onto superfrost slides and was losing about half of my sections. I ended up swapping to superfrost plus ultra, plus I bake the slides in the histology oven at 60 degress celcius for 10 minutes and now I nearly never lose a section - the only time I do is when I have done a lazy job of cutting and have collected a section with a fold in it. Good luck! > > Message: 9 > Date: Wed, 09 Dec 2009 11:40:50 -0800 > From: Angelina Fong > Subject: [Histonet] Help! Losing sections from Superfrost Plus Slides > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: <4B1FFD42.2040406@zoology.ubc.ca> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi all, > > We have suddenly started losing tissue sections from our Superfrost Plus > Slides. > > Our students have been cutting fixed, frozen, cryosections (20um) and > thaw-mounting these onto Superfrost Plus slides. We have suddenly > started losing lots of sections from these slides (again!!). The tissue > is small - ie cross sections of frog aorta and longitudinal sections of > nerves, so any lose of adhesion results in total loss of the tissue. > The odd thing is that she is not losing every section on every slide, > but half to 3/4 of the sections are falling off within the first rinse. > The sections are from the same tissue block on the same slide while some > falls and others don't. > > We are at a lose as to what we can do to rescue these sections. > > Does anyone know if there is any way to coat the slides in some solution > with the tissue on them to help improve the adhesion without losing the > ability to do immunofluorescence? > > Any further advice on cutting / drying protocols are welcomed. > > This keeps happening and the inconsistency of it has us so frustrated > with this that we are thinking of going back to subbing our own slides. > > Thanks for your help! > > Angelina > > > -- > ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o > > Angelina Y. Fong, Ph.D. > Department of Zoology > Biological Sciences Building > 6270 University Boulevard > University of British Columbia > Vancouver, BC, V6T 1Z4 > Canada > > Ph: (604) 822-5799 > Fax: (604) 822-2416 > Email: fong@zoology.ubc.ca > > > > > > ------------------------------ > > Message: 10 > Date: Wed, 9 Dec 2009 15:08:57 -0500 > From: HistoLab > Subject: [Histonet] Help! Losing sections from Superfrost Plus Slides > To: histonet@lists.utsouthwestern.edu > Message-ID: > <521c6d260912091208n421bf467g3240121cd26f06a0@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Angelina, > > I have seen this post a few times before and sometimes the problem was the > drying technique or making sure your water bath is clean and free of any > debris. I also remember hearing about someone pre-treating their slides with > a Trilogy (EDTA) buffer in a pressure cooker. > > Good Luck! > > *Matthew Semovoski* > Sales Manager > Gorilla Scientific Corporation > 1-866-435-4977 > www.gorillascientific.com _________________________________________________________________ Get more out of Hotmail Check out the latest features today http://windowslive.ninemsn.com.au/hotmail/article/878466/your-hotmail-is-about-to-get-even-better From sharon.osborn <@t> comcast.net Thu Dec 10 01:17:04 2009 From: sharon.osborn <@t> comcast.net (Sharon Osborn) Date: Thu Dec 10 01:17:06 2009 Subject: [Histonet] Leitz 1512 Message-ID: <00fb01ca7968$c6c57730$54506590$@osborn@comcast.net> Several of you have recently written wanting the instructions for the Leitz 1512. I had temporarily misplaced these because my lab moved for the second time in the past year. I have now located these. Please re-connect with me so I can fax or mail you these instructions. Thank you. Sharon Osborn From annigyg <@t> gmail.com Thu Dec 10 05:27:00 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Dec 10 05:27:07 2009 Subject: [Histonet] Overstaining - Mayers H&E In-Reply-To: References: Message-ID: Well said John - *eats grass and leaves speaks to the hand* AbuDhabiAnnie 2009/12/9 John Kiernan > This is a typical example of the informal "protocols" that get passed on > from generation to generation of graduate students, postdocs and technicians > in research labs at universities. The original was probably written by > someone who knew how to do H&E staining, but on differently fixed tissues, > and certainly on thinner sections. It apears to be for individual slides, > because 10 seconds in each of the two 95% and 100% ethanols would be > effective only with vigorous agitation in a large excess of fluid. > > The tissue is almost unfixed, unless "Soak in 0.4% paraformaldehyde" means > leave it ovenight or longer in 4% formaldehyde. > Researchers otherwise educated to the highest levels in such difficult > disciplines as molecular biology and neuroscience regularly write phrases > like "4% paraformaldehyde", thereby advertising their profound ignorance > about fixation, which is the procedure that has the greatest effect on the > appearance of anything dead that's examined with a microscope, especially if > stains or histochemical methods are to be used. (I apologise for the length > of the preceding sentence, but not for its punctuation, which is correct in > British but not in American English usage. Check it out with Lynne Truss!) > > The "sucrose cycle" step, with no times or instructions about floating and > sinking, is probably local jargon from a lab where small animals' brains are > minimally fixed and cryoprotected before cutting thick (50-100um) frozen > sections, to be stained free-floating. That's not an H&E job! You are > working with a thin skeletal muscle (rat's gastrocnemius). > > If your Mayer's haemalum is a bought solution, it is intended for use in > hospital labs, on paraffin sections about 5um thick. In a research setting > you may need to make changes. Haemalum (Mayer's or anyone else's, correctly > used) should stain cell nuclei blue and very little else. > > An important part of H&E staining is looking at the wet section with a > microscope to check for adequate and selective nuclear coloration. In > skeletal muscle the nuclei are small, so the haemalum-stained section is > very pale blue to the unaided eye. With alcoholic eosin (as in your method) > it's not so easy to control the staining with microscopic control, but it > will probably be OK if the section is light pink. Some people like their > eosin darker; it's largely a matter of taste unless you need to distinguish > between different eosinophilic components on the basis of hue. > > John Kiernan > Anatomy, UWO > London, Canada > = = = > -----Original Message ----- > From: Josephine Garcia > Date: Monday, December 7, 2009 11:43 > Subject: [Histonet] Overstaining - Mayers H&E > To: histonet@lists.utsouthwestern.edu > > > Hi all, > > > > My (frozen-section, fixed) slides are coming out much too dark > > (overstainedpurple) and I'm not sure why. They are 15-20 > > micrometer slices of rat > > gastrocnemius muscle. Can someone please look over our current > > protocol and > > tell me what I'm doing wrong? Thanks! Here it is: > > > > 1. Perfuse animal with 4% paraformaldehyde fixative. > > 2. Soak in 0.4% paraformaldehyde > > 3. Sucrose cycle (5% rinse, 10%, 20%, 30% soak) > > 4. Embed in OCT, Frozen sections (15-20 micrometers) > > 5. Let dry for 15-30 min > > 6. Stain as follows: > > > > - Distilled H2O (quick dip) > > - Mayer's Hematoxylin - 1min (originally we were dipping for 5- > > 10 minutes. I > > slowly reduced the time to 2min, then 1min, then 30s... still > > overstained!)- Running lukewarm tap water - drain and > > continuously fill - 15min or until > > water runs clear > > - Distilled H2O (quick dip) > > - 80% EtOH - 1-2min > > - Eosin - 2 min > > - 95% EtOH I - 10sec > > - 95% EtOH II - 10sec > > - 100% EtOH I - 10sec > > - 100% EtOH II - 10sec > > - Xylene I - 2min > > - Xylene II - 2min > > > > 7. Coverslip and let dry overnight > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From rjbuesa <@t> yahoo.com Thu Dec 10 07:55:26 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 10 07:55:33 2009 Subject: [Histonet] maximum block cutting In-Reply-To: <52093.68.178.72.125.1260396457.squirrel@webmail.integra.net> Message-ID: <276323.43639.qm@web65705.mail.ac4.yahoo.com> The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/hour?with an average of 24 (sample size = 245 histolabs). Ren? J. --- On Wed, 12/9/09, histology@medsurgpath.com wrote: From: histology@medsurgpath.com Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Thu Dec 10 08:50:11 2009 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Thu Dec 10 08:50:20 2009 Subject: [Histonet] Overstaining - Mayers H&E In-Reply-To: References: Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8C9F7E005@EXC-MBX3.cfs.le.ac.uk> Spot on!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: 09 December 2009 17:10 To: Josephine Garcia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Overstaining - Mayers H&E This is a typical example of the informal "protocols" that get passed on from generation to generation of graduate students, postdocs and technicians in research labs at universities. The original was probably written by someone who knew how to do H&E staining, but on differently fixed tissues, and certainly on thinner sections. It apears to be for individual slides, because 10 seconds in each of the two 95% and 100% ethanols would be effective only with vigorous agitation in a large excess of fluid. The tissue is almost unfixed, unless "Soak in 0.4% paraformaldehyde" means leave it ovenight or longer in 4% formaldehyde. Researchers otherwise educated to the highest levels in such difficult disciplines as molecular biology and neuroscience regularly write phrases like "4% paraformaldehyde", thereby advertising their profound ignorance about fixation, which is the procedure that has the greatest effect on the appearance of anything dead that's examined with a microscope, especially if stains or histochemical methods are to be used. (I apologise for the length of the preceding sentence, but not for its punctuation, which is correct in British but not in American English usage. Check it out with Lynne Truss!) The "sucrose cycle" step, with no times or instructions about floating and sinking, is probably local jargon from a lab where small animals' brains are minimally fixed and cryoprotected before cutting thick (50-100um) frozen sections, to be stained free-floating. That's not an H&E job! You are working with a thin skeletal muscle (rat's gastrocnemius). If your Mayer's haemalum is a bought solution, it is intended for use in hospital labs, on paraffin sections about 5um thick. In a research setting you may need to make changes. Haemalum (Mayer's or anyone else's, correctly used) should stain cell nuclei blue and very little else. An important part of H&E staining is looking at the wet section with a microscope to check for adequate and selective nuclear coloration. In skeletal muscle the nuclei are small, so the haemalum-stained section is very pale blue to the unaided eye. With alcoholic eosin (as in your method) it's not so easy to control the staining with microscopic control, but it will probably be OK if the section is light pink. Some people like their eosin darker; it's largely a matter of taste unless you need to distinguish between different eosinophilic components on the basis of hue. John Kiernan Anatomy, UWO London, Canada = = = -----Original Message ----- From: Josephine Garcia Date: Monday, December 7, 2009 11:43 Subject: [Histonet] Overstaining - Mayers H&E To: histonet@lists.utsouthwestern.edu > Hi all, > > My (frozen-section, fixed) slides are coming out much too dark > (overstainedpurple) and I'm not sure why. They are 15-20 > micrometer slices of rat > gastrocnemius muscle. Can someone please look over our current > protocol and > tell me what I'm doing wrong? Thanks! Here it is: > > 1. Perfuse animal with 4% paraformaldehyde fixative. > 2. Soak in 0.4% paraformaldehyde > 3. Sucrose cycle (5% rinse, 10%, 20%, 30% soak) > 4. Embed in OCT, Frozen sections (15-20 micrometers) > 5. Let dry for 15-30 min > 6. Stain as follows: > > - Distilled H2O (quick dip) > - Mayer's Hematoxylin - 1min (originally we were dipping for 5- > 10 minutes. I > slowly reduced the time to 2min, then 1min, then 30s... still > overstained!)- Running lukewarm tap water - drain and > continuously fill - 15min or until > water runs clear > - Distilled H2O (quick dip) > - 80% EtOH - 1-2min > - Eosin - 2 min > - 95% EtOH I - 10sec > - 95% EtOH II - 10sec > - 100% EtOH I - 10sec > - 100% EtOH II - 10sec > - Xylene I - 2min > - Xylene II - 2min > > 7. Coverslip and let dry overnight > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DunlapC <@t> umkc.edu Thu Dec 10 09:56:08 2009 From: DunlapC <@t> umkc.edu (Dunlap, Charles) Date: Thu Dec 10 09:56:14 2009 Subject: [Histonet] time tissue stays in paraffin Message-ID: <032EC4F75A527A4FA58C5B1B5DECFBB305084136@KC-MSX1.kc.umkc.edu> We have a software glitch in our tissue processor, it stops in the first paraffin and will not progress to paraffins 2 and 3 then complete the cycle. If we catch it quickly, we can instruct it to continue but the cycle happens around 5:00 am so we sometimes are not here, tissuet may spend an extra 30 minutes or even an hour in the first paraffin. Estimated cost of repairing is a whopping 6 thousand $$. Does this have a major effect on quality of slides....sometimes ours are less than ideal but I am not sure if the problem is in the paraffin cycle or elsewhere...sectioning, staining, etc. Any ideas? Charles Dunlap From 41dmb41 <@t> gmail.com Thu Dec 10 09:58:30 2009 From: 41dmb41 <@t> gmail.com (Drew Meyer) Date: Thu Dec 10 09:58:44 2009 Subject: [Histonet] time tissue stays in paraffin In-Reply-To: <032EC4F75A527A4FA58C5B1B5DECFBB305084136@KC-MSX1.kc.umkc.edu> References: <032EC4F75A527A4FA58C5B1B5DECFBB305084136@KC-MSX1.kc.umkc.edu> Message-ID: What is the make/model of your tissue processor? Drew Sent from my iPhone On Dec 10, 2009, at 10:56 AM, "Dunlap, Charles" wrote: > We have a software glitch in our tissue processor, it stops in the > first paraffin and will not progress to paraffins 2 and 3 then > complete > the cycle. If we catch it quickly, we can instruct it to continue > but > the cycle happens around 5:00 am so we sometimes are not here, tissuet > may spend an extra 30 minutes or even an hour in the first paraffin. > Estimated cost of repairing is a whopping 6 thousand $$. Does this > have a major effect on quality of slides....sometimes ours are less > than > ideal but I am not sure if the problem is in the paraffin cycle or > elsewhere...sectioning, staining, etc. Any ideas? Charles Dunlap > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Dec 10 10:03:14 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 10 10:03:19 2009 Subject: [Histonet] time tissue stays in paraffin In-Reply-To: <032EC4F75A527A4FA58C5B1B5DECFBB305084136@KC-MSX1.kc.umkc.edu> Message-ID: <82016.65288.qm@web65713.mail.ac4.yahoo.com> If this a frequent happening and the tissues stay an extra 30 to 60 minutes, it is not a big deal. You could to 2 things: 1- set the tissue processor "in delay" mode so it starts 30 to 60 minutes later,and by doing so the tissue will stay from 30-60 minutes extra in NBF, which is good, or 2- program in a way? that the tissue will stay in paraffin 2 and 3 a total of 30-60 mins less, and your tissue will stay the same amount of time in paraffin as if they were not stuck in station 1. But, again, that is not a big deal. Ren? J. --- On Thu, 12/10/09, Dunlap, Charles wrote: From: Dunlap, Charles Subject: [Histonet] time tissue stays in paraffin To: histonet@lists.utsouthwestern.edu Date: Thursday, December 10, 2009, 10:56 AM We have a software glitch in our tissue processor, it? stops in the first paraffin and will not progress to paraffins 2 and 3 then complete the cycle.???If we catch it quickly, we can instruct it to continue but the cycle happens around 5:00 am so we sometimes are not here, tissuet may spend an extra 30 minutes or even an hour in the first paraffin. Estimated cost of repairing is a whopping? 6 thousand $$.? ? Does this have a major effect on quality of slides....sometimes ours are less than ideal but I am not sure if the problem is in the paraffin cycle or elsewhere...sectioning, staining, etc.? Any ideas????Charles Dunlap _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfitz <@t> 007group.com Thu Dec 10 11:22:07 2009 From: cfitz <@t> 007group.com (Cathy) Date: Thu Dec 10 11:22:11 2009 Subject: [Histonet] maximum block cutting In-Reply-To: <276323.43639.qm@web65705.mail.ac4.yahoo.com> References: <52093.68.178.72.125.1260396457.squirrel@webmail.integra.net> <276323.43639.qm@web65705.mail.ac4.yahoo.com> Message-ID: Does this average include trimming into the block or just the actual cutting of the section? Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com Subject: Re: [Histonet] maximum block cutting The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/hour?with an average of 24 (sample size = 245 histolabs). Ren? J. --- On Wed, 12/9/09, histology@medsurgpath.com wrote: From: histology@medsurgpath.com Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Thu Dec 10 11:21:04 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Dec 10 11:22:55 2009 Subject: [Histonet] RE: time tissue stays in paraffin In-Reply-To: <032EC4F75A527A4FA58C5B1B5DECFBB305084136@KC-MSX1.kc.umkc.edu> References: <032EC4F75A527A4FA58C5B1B5DECFBB305084136@KC-MSX1.kc.umkc.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E39098E3497E2@IBMB7Exchange.digestivespecialists.com> Is it possible to bypass that station completely? Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dunlap, Charles Sent: Thursday, December 10, 2009 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] time tissue stays in paraffin We have a software glitch in our tissue processor, it stops in the first paraffin and will not progress to paraffins 2 and 3 then complete the cycle. If we catch it quickly, we can instruct it to continue but the cycle happens around 5:00 am so we sometimes are not here, tissuet may spend an extra 30 minutes or even an hour in the first paraffin. Estimated cost of repairing is a whopping 6 thousand $$. Does this have a major effect on quality of slides....sometimes ours are less than ideal but I am not sure if the problem is in the paraffin cycle or elsewhere...sectioning, staining, etc. Any ideas? Charles Dunlap _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jeannette.Mitchell <@t> vtmednet.org Thu Dec 10 11:44:10 2009 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Thu Dec 10 11:44:15 2009 Subject: [Histonet] microtomes for sale Message-ID: Hi - We have three auto-microtomes available and want to sell two and give the 3rd to a Histology lab in another country. One is a Thermo Electron Finesse, high profile blade holder, foot pedal and side key pad, quick release block clamp, circa 2003. Good working condition. $4000 or best offer The other two are Leica RM 2155s, high profile blade holder, foot pedal, quick release block clamp, circa 1998 and 2000. Good working condition. We only will sell one of the two. Need the other for back-up. $2000 or best offer. All require the buyer to arrange and pay for shipping. Please contact me if you are interested. thanks Jude Jude Carpenter, BS, HTL (ASCP) Supervisor of Histology/Surgical Pathology/Autopsy Fletcher Allen Healthcare EP2-101/ACC 111 Colchester Ave. Burlington, VT 05401 (802)847-5116 FAX (802)847-4155 jude.carpenter@vtmednet.org From algranth <@t> email.arizona.edu Thu Dec 10 12:02:58 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Thu Dec 10 12:03:07 2009 Subject: [Histonet] arizona state license In-Reply-To: References: Message-ID: NO Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello P Please consider the environment before printing this email. On Dec 9, 2009, at 4:38 PM, wrote: > Does anyone know if Arizona requires a state license for > histotechnicians/technologists? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dellav <@t> musc.edu Thu Dec 10 13:33:03 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Dec 10 13:33:48 2009 Subject: [Histonet] Ventana Stainer In-Reply-To: <972367.10090.qm@web112812.mail.gq1.yahoo.com> References: <972367.10090.qm@web112812.mail.gq1.yahoo.com> Message-ID: Jimmy has informed me that he resides in the Cleveland area. Can anyone in that area that has a Ventana stainer permit him a visit ?? Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jimmy A Sent: Tuesday, December 08, 2009 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Stainer ? ? ?? ????? Hi ? ? I am looking for a lab that can allow me to spend one or two days to properly see how the ventana autostainer?works. ???? I am an histotech working here?in the US. I am interested in learning? the operation of the ventana immunostainers. I will appreciate it, if one of the?histology/ihc labs could?grant me?this great favour. ???? ?Hoping to hear from you guys asap. ? ???? Jimmy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Thu Dec 10 14:32:09 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Dec 10 14:32:15 2009 Subject: [Histonet] fish scales Message-ID: We are trying to cut fish scales that have been decalcified. They are chunking out when we try to cut them. I think we need to soften the keratin and I looked in the archives for the right dilution of ammonium hydroxide to use. One post said 5% the other said straight. What dilution do you recommend? I'm think it would probably be the same as what you use on toenails. Margaret Perry HT(ASCP) From gayle.callis <@t> bresnan.net Thu Dec 10 14:49:37 2009 From: gayle.callis <@t> bresnan.net (gayle.callis@bresnan.net) Date: Thu Dec 10 14:49:13 2009 Subject: [Histonet] fish scales In-Reply-To: References: Message-ID: <825755352-1260478149-cardhu_decombobulator_blackberry.rim.net-1012507121-@bda420.bisx.prod.on.blackberry> Are fish scales even calcified? If so decalcifying may not work. If keratin - softening with ammonia water, Mollifex(sp?), Nair which is alkaline. I would be more tempted to use GMA plastic to match hardness of scales. I have a protocol for horse hooves. If you want will send privately. This is also in HistoLogic, author is Jane Chladny. Gayle Callis Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Perry, Margaret" Date: Thu, 10 Dec 2009 12:32:09 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fish scales We are trying to cut fish scales that have been decalcified. They are chunking out when we try to cut them. I think we need to soften the keratin and I looked in the archives for the right dilution of ammonium hydroxide to use. One post said 5% the other said straight. What dilution do you recommend? I'm think it would probably be the same as what you use on toenails. Margaret Perry HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Dec 10 15:29:06 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 10 15:29:11 2009 Subject: [Histonet] maximum block cutting In-Reply-To: Message-ID: <323116.31981.qm@web65702.mail.ac4.yahoo.com> Just actual cutting. Trimming is considered another task. Ren? J. --- On Thu, 12/10/09, Cathy wrote: From: Cathy Subject: RE: [Histonet] maximum block cutting To: "'Rene J Buesa'" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com Date: Thursday, December 10, 2009, 12:22 PM Does this average include trimming into the block or just the actual cutting of the section? Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com Subject: Re: [Histonet] maximum block cutting The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/hour?with an average of 24 (sample size = 245 histolabs). Ren? J. --- On Wed, 12/9/09, histology@medsurgpath.com wrote: From: histology@medsurgpath.com Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison_Scott <@t> hchd.tmc.edu Thu Dec 10 15:41:03 2009 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Thu Dec 10 15:41:08 2009 Subject: [Histonet] Fungus/PCP controls Message-ID: <1872B4A455B7974391609AD8034C79FC8BD66C@LBEXCH01.hchd.local> Hello to all in histoland. Is there anyone willing to share a fungus and a pcp block with me. We always run a fungus and pcp control slide that has both tissues on it for our cytology cases. Our blocks are depleted. I also have commerical controls for fungus and pcp. We just have a control slide with both for our cytology cases. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 5656 Kelley Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From khbarr <@t> mdanderson.org Thu Dec 10 15:42:17 2009 From: khbarr <@t> mdanderson.org (Barr,Kaye H) Date: Thu Dec 10 15:42:25 2009 Subject: [Histonet] HT & PA's Needed Message-ID: The University of Texas M. D. Anderson Cancer Center in Houston, Texas is searching for a full time Clinical Histology Technician, night shift (11:00 pm - 7:00 am) and a full time Pathologist Assistant, M-F days. Instereted candidates can apply online @ mdanderson.org or contact our HR recruiter - Dianna Menard @ 713-745-6184 or email her dmenard@mdanderson.org. Kaye Barr, HT(ASCP) Laboratory Manager, PLM Pathology Dept. 713-792-5366 From Herrick.James <@t> mayo.edu Thu Dec 10 16:40:10 2009 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Thu Dec 10 16:40:18 2009 Subject: [Histonet] MMA embedding issue Message-ID: <7267A64D75F58241B577876D8A885631015A98C8@msgebe41> Hello everyone, I have embedded several rat femur/tibia specimens in MMA and am finding large holes (un-polymerized tissue) as I cut into the tissue - I am still in the soft tissue and not even into the bone yet (the specimens have large tumors). I placed a couple of the specimens into a 50?C oven for approximately 24 hours and then back into MMA for about a week with fresh changes and 60 minute vacuums each day. After cutting into the specimen again (following re-embedding), I am still finding soft un-polymerized tissue. Can I take these back to the liquid state with pure Methyl Methacrylate, then back into absolute alcohol, and once again back into methacrylate? I have done a large number of specimens similar and larger than these over the past few years without any trouble. If anyone has any knowledge and/or experience with this issue, your sharing would be greatly appreciated. Thanks again. Jim Herrick Senior Research Technologist Department of Orthopedics Bone Histomorphometry Core Lab Phone: (507) 255-5946 fax: (507) 266-9451 Email: herrick.james@mayo.edu ______________________ Mayo Clinic 200 First Street SW Rochester, MN 55905 www.mayoclinic.org From kiran_g <@t> sbcglobal.net Thu Dec 10 17:34:45 2009 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Thu Dec 10 17:34:49 2009 Subject: [Histonet] Histonet]Dual IHC stain for p16 and Ki67 on cytology specimens Message-ID: <296839.8007.qm@web180112.mail.gq1.yahoo.com> I?am intersted in getting some info if anyone in histo world is?doing dual IHC stain for p16 &?Ki67?on cytology specimens.?What kind of platform and antibodies?your are using ? Also if you can share your procedure that will be awesome. ? Thank you in advance, ? Happy Holidays to All. ? ? ? From Kim.Osullivan <@t> med.monash.edu.au Thu Dec 10 18:03:41 2009 From: Kim.Osullivan <@t> med.monash.edu.au (Kim O'Sullivan) Date: Thu Dec 10 18:03:50 2009 Subject: [Histonet] non specific staining Message-ID: <130.194.114.97.1260489292@my.monash.edu.au> Hi, I am having a problem using DAB black on snap frozen mouse kidneys. We routinely stain for leukocytes (CD4(Gk1.5),neutrophils(GR1), and macrophages (fA11)) on PLP fixed tissue with very little background staining. However we currrently have to stain some snap tissue as the PLP tissue is all gone. The problem we are having is some cells are staining very specifically on the isotype controls, and controls with no primary or secondary antibody-this population of cells are hard to distinguish from positive cells in the experimental tissue. I presume it is the DAB chromagen that is binding very specifically to a certain cell population. We currently use 0.3% H202 in methanol for 30 minutes to block endogenous peroxidase- is this insufficient? Does anyone think it is an endogenous peroxidase problem or something else? Any suggestions would be helpful, Kim Department Of Medicine Monash University Monash Medical Centre Melbourne, Australia From trathborne <@t> somerset-healthcare.com Fri Dec 11 08:03:51 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Dec 11 08:03:58 2009 Subject: [Histonet] maximum block cutting In-Reply-To: <323116.31981.qm@web65702.mail.ac4.yahoo.com> Message-ID: So what would the time be for trimming and cutting? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 4:29 PM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com; Cathy Subject: RE: [Histonet] maximum block cutting Just actual cutting. Trimming is considered another task. Ren? J. --- On Thu, 12/10/09, Cathy wrote: From: Cathy Subject: RE: [Histonet] maximum block cutting To: "'Rene J Buesa'" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com Date: Thursday, December 10, 2009, 12:22 PM Does this average include trimming into the block or just the actual cutting of the section? Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com Subject: Re: [Histonet] maximum block cutting The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/hour?with an average of 24 (sample size = 245 histolabs). Ren? J. --- On Wed, 12/9/09, histology@medsurgpath.com wrote: From: histology@medsurgpath.com Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From BoozerKA <@t> ah.org Fri Dec 11 08:08:08 2009 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Fri Dec 11 08:08:30 2009 Subject: [Histonet] Leica vs Ventana Message-ID: <4B21E1C5.4AA8.00C0.0@ah.org> Anyone out there found an advantage of going from Ventana to Leica for IHC? I am being warned about extra costs and protocol issues with the 3 trays. In negotiations.... Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org From cmiller <@t> physlab.com Fri Dec 11 08:28:05 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Dec 11 08:28:12 2009 Subject: [Histonet] Leica vs Ventana In-Reply-To: <4B21E1C5.4AA8.00C0.0@ah.org> References: <4B21E1C5.4AA8.00C0.0@ah.org> Message-ID: Why the switch? I am a big fan of Ventana. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From LSebree <@t> uwhealth.org Fri Dec 11 08:40:31 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Dec 11 08:40:37 2009 Subject: [Histonet] Leica vs Ventana In-Reply-To: <4B21E1C5.4AA8.00C0.0@ah.org> Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF6DC@UWHC-MAIL01.uwhis.hosp.wisc.edu> Why would you switch from a reliable, walk-away, reproducible system? Just curious. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Friday, December 11, 2009 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica vs Ventana Anyone out there found an advantage of going from Ventana to Leica for IHC? I am being warned about extra costs and protocol issues with the 3 trays. In negotiations.... Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From araniqkslvr <@t> yahoo.com Fri Dec 11 08:41:36 2009 From: araniqkslvr <@t> yahoo.com (Paula) Date: Fri Dec 11 08:41:40 2009 Subject: [Histonet] Seeking Position Message-ID: <662872.68940.qm@web30403.mail.mud.yahoo.com> I am seeking a lab assistant/histology assistant position in North Carolina. I have HT Certification, which is still good, but my experience is old--from 1987/89, so I am looking for a place to start slow and learn.?I am willing to take courses if I need to and I don't mind being an assistant/helper--I have done it in the clerical field, too. ? We will be (hopefully) moving there in the next few weeks. I would like to start something as soon as possible after the new year. We are looking for somewhere to live between Rocky Mount (my boyfriend's mother lives there and he knows some of that area) and Raleigh. ? Other areas of the state are ok, too, but we don't know much about any of them. ? Paula From Ronald.Houston <@t> nationwidechildrens.org Fri Dec 11 08:44:06 2009 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Dec 11 08:44:17 2009 Subject: [Histonet] Leica vs Ventana In-Reply-To: <8C023B4AB999614BA4791BAEB26E27381BF6DC@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21B645E7E@chi2k3ms01.columbuschildrens.net> Not enough time in the day to answer that one Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, December 11, 2009 9:41 AM To: Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Why would you switch from a reliable, walk-away, reproducible system? Just curious. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Friday, December 11, 2009 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica vs Ventana Anyone out there found an advantage of going from Ventana to Leica for IHC? I am being warned about extra costs and protocol issues with the 3 trays. In negotiations.... Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From ree3 <@t> leicester.ac.uk Fri Dec 11 08:51:51 2009 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Fri Dec 11 08:52:54 2009 Subject: [Histonet] Leica vs Ventana In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB21B645E7E@chi2k3ms01.columbuschildrens.net> References: <8C023B4AB999614BA4791BAEB26E27381BF6DC@UWHC-MAIL01.uwhis.hosp.wisc.edu> <979FF5962E234F45B06CF0DB7C1AABB21B645E7E@chi2k3ms01.columbuschildrens.net> Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8C9F7E017@EXC-MBX3.cfs.le.ac.uk> This sounds like the game children play, so who would win the fight between an elephant and a hippopotamus, or the fight between a banana and a pomegranate etcetc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: 11 December 2009 14:44 To: Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Not enough time in the day to answer that one Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, December 11, 2009 9:41 AM To: Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Why would you switch from a reliable, walk-away, reproducible system? Just curious. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Friday, December 11, 2009 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica vs Ventana Anyone out there found an advantage of going from Ventana to Leica for IHC? I am being warned about extra costs and protocol issues with the 3 trays. In negotiations.... Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Fri Dec 11 08:55:10 2009 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Dec 11 08:55:40 2009 Subject: [Histonet] Leica vs Ventana In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1E8C9F7E017@EXC-MBX3.cfs.le.ac.uk> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21B645E7F@chi2k3ms01.columbuschildrens.net> Probably depends on whether you're talking about a white elephant or not Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: Edwards, Richard E. [mailto:ree3@leicester.ac.uk] Sent: Friday, December 11, 2009 9:52 AM To: Houston, Ronald; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana This sounds like the game children play, so who would win the fight between an elephant and a hippopotamus, or the fight between a banana and a pomegranate etcetc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: 11 December 2009 14:44 To: Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Not enough time in the day to answer that one Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, December 11, 2009 9:41 AM To: Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Why would you switch from a reliable, walk-away, reproducible system? Just curious. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Friday, December 11, 2009 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica vs Ventana Anyone out there found an advantage of going from Ventana to Leica for IHC? I am being warned about extra costs and protocol issues with the 3 trays. In negotiations.... Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Fri Dec 11 09:02:02 2009 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Dec 11 09:02:19 2009 Subject: [Histonet] Leica vs Ventana In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB21B645E7F@chi2k3ms01.columbuschildrens.net> References: <7722595275A4DD4FA225B92CDBF174A1E8C9F7E017@EXC-MBX3.cfs.le.ac.uk> <979FF5962E234F45B06CF0DB7C1AABB21B645E7F@chi2k3ms01.columbuschildrens.net> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A54B6@EXCHMBC2.ad.ah.local> I think these obscure emails about vendors are very damaging, regardless of who the vender is. If someone is experiencing a particular problem with an instrument, please use this venue to get advice on how others handled it. Be open about the problem so that we all can learn from your experiences. To dog a company without any real information is not useful to anyone. Both companies have good instruments that may suit different types of labs better than the other. I'll get off my soap box now. Jan Mahoney, Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Friday, December 11, 2009 8:55 AM To: Edwards, Richard E.; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Probably depends on whether you're talking about a white elephant or not Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: Edwards, Richard E. [mailto:ree3@leicester.ac.uk] Sent: Friday, December 11, 2009 9:52 AM To: Houston, Ronald; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana This sounds like the game children play, so who would win the fight between an elephant and a hippopotamus, or the fight between a banana and a pomegranate etcetc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: 11 December 2009 14:44 To: Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Not enough time in the day to answer that one Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, December 11, 2009 9:41 AM To: Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Why would you switch from a reliable, walk-away, reproducible system? Just curious. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Friday, December 11, 2009 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica vs Ventana Anyone out there found an advantage of going from Ventana to Leica for IHC? I am being warned about extra costs and protocol issues with the 3 trays. In negotiations.... Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. 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From sbreeden <@t> nmda.nmsu.edu Fri Dec 11 09:08:29 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Dec 11 09:09:31 2009 Subject: [Histonet] Equipment vs Equipment Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46CD0@nmdamailsvr.nmda.ad.nmsu.edu> I agree with Janice Mahoney - if you need input on a certain piece of equipment or procedure, post it and ask those that choose to reply to respond to you directly. I don't like to see "bashing" on anything (I'm a pacifist, I guess) but have responded to several posts asking for opinions directly to the inquirer. Many different manufacturers offer great products - it depends on how you will use it. And on that note, I'd like to repeat my original Histonet posting from several years ago... why couldn't manufacturers of plastic bottles (particularly the liter/gallon size) modify the pouring lip so the liquid you are pouring doesn't run and drip? That's about as critical as I can manage today and it's pretty generic. Peace out! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From JCBRITTON <@t> Cheshire-Med.COM Fri Dec 11 09:30:42 2009 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Fri Dec 11 09:30:49 2009 Subject: [Histonet] fish scales In-Reply-To: References: Message-ID: Nair hair remover works great on toenails! Josie Britton HT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Thursday, December 10, 2009 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fish scales We are trying to cut fish scales that have been decalcified. They are chunking out when we try to cut them. I think we need to soften the keratin and I looked in the archives for the right dilution of ammonium hydroxide to use. One post said 5% the other said straight. What dilution do you recommend? I'm think it would probably be the same as what you use on toenails. Margaret Perry HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From arvidsonkristen <@t> yahoo.com Fri Dec 11 09:30:45 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Fri Dec 11 09:30:53 2009 Subject: [Histonet] reprocessing small pieces of tissue Message-ID: <59120.37661.qm@web65703.mail.ac4.yahoo.com> Has anyone re-processed a small pc. of tissue before?? if so how, do you do it? ? In general, how are you re-processing tissue? From arvidsonkristen <@t> yahoo.com Fri Dec 11 09:32:29 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Fri Dec 11 09:32:33 2009 Subject: [Histonet] Injuries in the Lab Message-ID: <827428.56106.qm@web65713.mail.ac4.yahoo.com> How are people dealing with injuries like cuts from the microtome blade or cuts at the grossing bench (fixed tissue only)?? We do not work in a hospital so we do not have employee health. From JCBRITTON <@t> Cheshire-Med.COM Fri Dec 11 09:34:46 2009 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Fri Dec 11 09:34:52 2009 Subject: [Histonet] fish scales In-Reply-To: References: Message-ID: Oh, I forgot to mention we do this after we face the block. We put a little swirl on the block face and let it soak for 10-30+ minutes depending on spec. You can usually get a ribbon, then you have to re-soak for another ribbon. I hope this helps! Josie Britton HT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Josie Britton Sent: Friday, December 11, 2009 10:31 AM To: Perry, Margaret; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] fish scales Nair hair remover works great on toenails! Josie Britton HT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Thursday, December 10, 2009 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fish scales We are trying to cut fish scales that have been decalcified. They are chunking out when we try to cut them. I think we need to soften the keratin and I looked in the archives for the right dilution of ammonium hydroxide to use. One post said 5% the other said straight. What dilution do you recommend? I'm think it would probably be the same as what you use on toenails. Margaret Perry HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From KGroeger <@t> USLABS.net Fri Dec 11 09:48:36 2009 From: KGroeger <@t> USLABS.net (Karin Groeger) Date: Fri Dec 11 09:48:41 2009 Subject: [Histonet] Leica vs Ventana In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A54B6@EXCHMBC2.ad.ah.local> References: <7722595275A4DD4FA225B92CDBF174A1E8C9F7E017@EXC-MBX3.cfs.le.ac.uk><979FF5962E234F45B06CF0DB7C1AABB21B645E7F@chi2k3ms01.columbuschildrens.net> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A54B6@EXCHMBC2.ad.ah.local> Message-ID: We are a large Reference Lab and use both instruments and get good results and vendor support from Leica and Ventana, if you are a small lab then it should be your preference and what you feel comfortable with. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Friday, December 11, 2009 7:02 AM To: 'Houston, Ronald'; Edwards,Richard E.; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana I think these obscure emails about vendors are very damaging, regardless of who the vender is. If someone is experiencing a particular problem with an instrument, please use this venue to get advice on how others handled it. Be open about the problem so that we all can learn from your experiences. To dog a company without any real information is not useful to anyone. Both companies have good instruments that may suit different types of labs better than the other. I'll get off my soap box now. Jan Mahoney, Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Friday, December 11, 2009 8:55 AM To: Edwards, Richard E.; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Probably depends on whether you're talking about a white elephant or not Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: Edwards, Richard E. [mailto:ree3@leicester.ac.uk] Sent: Friday, December 11, 2009 9:52 AM To: Houston, Ronald; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana This sounds like the game children play, so who would win the fight between an elephant and a hippopotamus, or the fight between a banana and a pomegranate etcetc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: 11 December 2009 14:44 To: Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Not enough time in the day to answer that one Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, December 11, 2009 9:41 AM To: Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Why would you switch from a reliable, walk-away, reproducible system? Just curious. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Friday, December 11, 2009 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica vs Ventana Anyone out there found an advantage of going from Ventana to Leica for IHC? I am being warned about extra costs and protocol issues with the 3 trays. In negotiations.... Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. 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The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. From mwfolsom <@t> swcp.com Fri Dec 11 10:05:04 2009 From: mwfolsom <@t> swcp.com (Michael W. Folsom) Date: Fri Dec 11 10:06:09 2009 Subject: [Histonet] alternates to absolute ethanol Message-ID: <4B226DB0.5090300@swcp.com> Hi: I want to process some plant tissue for microtomy. When I was doing this many years ago the protocol I used involved absolute ethanol. Knowing that getting ethanol that is really anhydrous is hard I was wondering if folks were using stuff that was denatured with 55% absolute methanol and 5% isoproponol instead. I understand it has better shelf life but wondered about the addition of small mounts of methanol and isopropanol. Also, if anybody is using this can you recommend a supplier? Thanks - Mike Rio Grand Biological From rjbuesa <@t> yahoo.com Fri Dec 11 10:47:45 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 11 10:47:50 2009 Subject: [Histonet] maximum block cutting In-Reply-To: Message-ID: <190590.2820.qm@web65713.mail.ac4.yahoo.com> Cutting : average of 2.5 min/block ( = 24/hour) Trimming: average of 25 secs./block ( =144/hour) Ren? J. --- On Fri, 12/11/09, Rathborne, Toni wrote: From: Rathborne, Toni Subject: RE: [Histonet] maximum block cutting To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, "Cathy" Date: Friday, December 11, 2009, 9:03 AM So what would the time be for trimming and cutting? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 4:29 PM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com; Cathy Subject: RE: [Histonet] maximum block cutting Just actual cutting. Trimming is considered another task. Ren? J. --- On Thu, 12/10/09, Cathy wrote: From: Cathy Subject: RE: [Histonet] maximum block cutting To: "'Rene J Buesa'" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com Date: Thursday, December 10, 2009, 12:22 PM Does this average include trimming into the block or just the actual cutting of the section? Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com Subject: Re: [Histonet] maximum block cutting The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/hour?with an average of 24 (sample size = 245 histolabs). Ren? J. --- On Wed, 12/9/09, histology@medsurgpath.com wrote: From: histology@medsurgpath.com Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. 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Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From rjbuesa <@t> yahoo.com Fri Dec 11 11:03:10 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 11 11:03:15 2009 Subject: [Histonet] alternates to absolute ethanol In-Reply-To: <4B226DB0.5090300@swcp.com> Message-ID: <141761.25901.qm@web65715.mail.ac4.yahoo.com> Pure isopropyl = isopropanol = 2-propanol is a better dehydrant than ethanol. Try it. Ren? J. --- On Fri, 12/11/09, Michael W. Folsom wrote: From: Michael W. Folsom Subject: [Histonet] alternates to absolute ethanol To: histonet@lists.utsouthwestern.edu Date: Friday, December 11, 2009, 11:05 AM Hi: I want to process some plant tissue for microtomy.? When I was doing this many years ago the protocol I used involved absolute ethanol. Knowing that getting ethanol that is really anhydrous is hard I was wondering if folks were using stuff that was denatured with 55% absolute methanol and 5% isoproponol instead.? I understand it has better shelf life but wondered about the addition of small mounts of methanol and isopropanol. Also, if anybody is using this can you recommend a supplier? Thanks - Mike Rio Grand Biological _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwfolsom <@t> swcp.com Fri Dec 11 11:14:21 2009 From: mwfolsom <@t> swcp.com (Michael W. Folsom) Date: Fri Dec 11 11:15:23 2009 Subject: [Histonet] one more question about reagent alcohol Message-ID: <4B227DED.6030404@swcp.com> Folks: Thanks for the many quick responses to my question about using "reagent alcohol" instead of 100% ethanol. One further question - in plant histology its quite common to use alcohol in fixatives - i.e. FAA, FPA & Farmers. Does anybody have any experience in using 190 proof reagent alcohol in a fixative? I know it introduces small amounts of methanol and isopropanol into the "cocktail" but not sure if that really matters - Thanks for the advice/help! Mike Rio Grande Biological From Michael.Owen <@t> fda.hhs.gov Fri Dec 11 11:23:31 2009 From: Michael.Owen <@t> fda.hhs.gov (Owen, Michael P) Date: Fri Dec 11 11:28:26 2009 Subject: [Histonet] alternates to absolute ethanol In-Reply-To: <4B226DB0.5090300@swcp.com> References: <4B226DB0.5090300@swcp.com> Message-ID: <6B1915839B67AD46B88E6BE8F27376BB19FBCC@FMD3VS031.fda.gov> Dear Michael, Have you tried absolute methanol? The cost of the reagent should be less expensive than absolute ethanol and is easier to obtain than absolute ethanol (i.e. less legal restrictions than absolute ethanol that can be diverted to non-laboratory use). When fixing bacterial smears for a variety of staining methods, I have discovered absolute methanol works well and is often superior to heat fixation. Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From rjbuesa <@t> yahoo.com Fri Dec 11 11:36:01 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 11 11:36:08 2009 Subject: [Histonet] maximum block cutting In-Reply-To: <445413799-1260551459-cardhu_decombobulator_blackberry.rim.net-856900486-@bda047.bisx.prod.on.blackberry> Message-ID: <80542.74357.qm@web65701.mail.ac4.yahoo.com> It is to be published in January 2010. If you "accept" 24 blocks/hour = 2.5 min/block Ren? J. --- On Fri, 12/11/09, jellin@yumaregional.org wrote: From: jellin@yumaregional.org Subject: Re: [Histonet] maximum block cutting To: "Rene J Buesa" , histonet-bounces@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, "Cathy" , "ToniRathborne" Date: Friday, December 11, 2009, 12:10 PM When was this data gathered... Some of this sounds excessive like 2.5 minutes a block??? Are these based on complete manual process or is automation also taken into consideration.? Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Date: Fri, 11 Dec 2009 08:47:45 To: ; ; Cathy; ToniRathborne Subject: RE: [Histonet] maximum block cutting Cutting : average of 2.5 min/block ( = 24/hour) Trimming: average of 25 secs./block ( =144/hour) Ren? J. --- On Fri, 12/11/09, Rathborne, Toni wrote: From: Rathborne, Toni Subject: RE: [Histonet] maximum block cutting To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, "Cathy" Date: Friday, December 11, 2009, 9:03 AM So what would the time be for trimming and cutting? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 4:29 PM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com; Cathy Subject: RE: [Histonet] maximum block cutting Just actual cutting. Trimming is considered another task. Ren? J. --- On Thu, 12/10/09, Cathy wrote: From: Cathy Subject: RE: [Histonet] maximum block cutting To: "'Rene J Buesa'" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com Date: Thursday, December 10, 2009, 12:22 PM Does this average include trimming into the block or just the actual cutting of the section? Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com Subject: Re: [Histonet] maximum block cutting The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/hour?with an average of 24 (sample size = 245 histolabs). Ren? J. --- On Wed, 12/9/09, histology@medsurgpath.com wrote: From: histology@medsurgpath.com Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. From rjbuesa <@t> yahoo.com Fri Dec 11 11:40:15 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 11 11:40:18 2009 Subject: [Histonet] alternates to absolute ethanol In-Reply-To: <6B1915839B67AD46B88E6BE8F27376BB19FBCC@FMD3VS031.fda.gov> Message-ID: <436000.23257.qm@web65705.mail.ac4.yahoo.com> The concern with methanol would be its toxicity. Methanol (TWA=200 ppm)?is 5 times more toxic than ethanol (TWA=1000 ppm) and twice more toxic than 2-propanol (TWA=400ppm). Ren? J. --- On Fri, 12/11/09, Owen, Michael P wrote: From: Owen, Michael P Subject: RE: [Histonet] alternates to absolute ethanol To: "Histonet" Date: Friday, December 11, 2009, 12:23 PM Dear Michael, Have you tried absolute methanol? The cost of the reagent should be less expensive than absolute ethanol and is easier to obtain than absolute ethanol (i.e. less legal restrictions than absolute ethanol that can be diverted to non-laboratory use). When fixing bacterial smears for a variety of staining methods, I have discovered absolute methanol works well and is often superior to heat fixation. Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE? Bothell, WA 98021-4421 Phone: 425-483-4865? ???E-Mail: michael.owen@fda.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jellin <@t> yumaregional.org Fri Dec 11 11:10:58 2009 From: jellin <@t> yumaregional.org (jellin@yumaregional.org) Date: Fri Dec 11 11:51:12 2009 Subject: [Histonet] maximum block cutting In-Reply-To: <190590.2820.qm@web65713.mail.ac4.yahoo.com> References: <190590.2820.qm@web65713.mail.ac4.yahoo.com> Message-ID: <445413799-1260551459-cardhu_decombobulator_blackberry.rim.net-856900486-@bda047.bisx.prod.on.blackberry> When was this data gathered... Some of this sounds excessive like 2.5 minutes a block?? Are these based on complete manual process or is automation also taken into consideration. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Date: Fri, 11 Dec 2009 08:47:45 To: ; ; Cathy; ToniRathborne Subject: RE: [Histonet] maximum block cutting Cutting : average of 2.5 min/block ( = 24/hour) Trimming: average of 25 secs./block ( =144/hour) Ren? J. --- On Fri, 12/11/09, Rathborne, Toni wrote: From: Rathborne, Toni Subject: RE: [Histonet] maximum block cutting To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, "Cathy" Date: Friday, December 11, 2009, 9:03 AM So what would the time be for trimming and cutting? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 4:29 PM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com; Cathy Subject: RE: [Histonet] maximum block cutting Just actual cutting. Trimming is considered another task. Ren? J. --- On Thu, 12/10/09, Cathy wrote: From: Cathy Subject: RE: [Histonet] maximum block cutting To: "'Rene J Buesa'" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com Date: Thursday, December 10, 2009, 12:22 PM Does this average include trimming into the block or just the actual cutting of the section? Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com Subject: Re: [Histonet] maximum block cutting The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/hour?with an average of 24 (sample size = 245 histolabs). Ren? J. --- On Wed, 12/9/09, histology@medsurgpath.com wrote: From: histology@medsurgpath.com Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. From akemiat3377 <@t> yahoo.com Fri Dec 11 11:53:08 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Fri Dec 11 11:53:59 2009 Subject: [Histonet] Leica vs Ventana In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A54B6@EXCHMBC2.ad.ah.local> References: <7722595275A4DD4FA225B92CDBF174A1E8C9F7E017@EXC-MBX3.cfs.le.ac.uk> <979FF5962E234F45B06CF0DB7C1AABB21B645E7F@chi2k3ms01.columbuschildrens.net> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A54B6@EXCHMBC2.ad.ah.local> Message-ID: <227D3462-0D2D-4971-A0A8-34E5585839B3@yahoo.com> Hi Janice, I'm glad you brought up your point! It's amazing how people are. It just shows how narrow minded and unkind in their statements. Yes, Ventana is HUGE, but they have their faults as well! That being said, there is always room to build a better mouse trap and save money while doing it too! The new Bond is amazing and is capable of doing multiple IHC staining on (1) slide. It is also a open system. "Science never sleeps"! Thank you for speaking out, and hope you have a Wonderful Holiday Season! Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Dec 11, 2009, at 8:02 AM, Mahoney,Janice A wrote: > I think these obscure emails about vendors are very damaging, > regardless of who the vender is. If someone is experiencing a > particular problem with an instrument, please use this venue to get > advice on how others handled it. Be open about the problem so that > we all can learn from your experiences. To dog a company without > any real information is not useful to anyone. > Both companies have good instruments that may suit different types > of labs better than the other. > I'll get off my soap box now. > Jan Mahoney, Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald > Sent: Friday, December 11, 2009 8:55 AM > To: Edwards, Richard E.; Sebree Linda A; Kathleen Boozer; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > Probably depends on whether you're talking about a white elephant > or not > > Ronnie Houston > Anatomic Pathology Manager > Nationwide Children's Hospital > Columbus OH 43205 > (614) 722 5450 > > -----Original Message----- > From: Edwards, Richard E. [mailto:ree3@leicester.ac.uk] > Sent: Friday, December 11, 2009 9:52 AM > To: Houston, Ronald; Sebree Linda A; Kathleen Boozer; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > This sounds like the game children play, so who would win the fight > between an elephant and a hippopotamus, or the fight between a banana > and a pomegranate etcetc. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Houston, > Ronald > Sent: 11 December 2009 14:44 > To: Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > Not enough time in the day to answer that one > > Ronnie Houston > Anatomic Pathology Manager > Nationwide Children's Hospital > Columbus OH 43205 > (614) 722 5450 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree > Linda A > Sent: Friday, December 11, 2009 9:41 AM > To: Kathleen Boozer; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > Why would you switch from a reliable, walk-away, reproducible system? > > Just curious. > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Kathleen > Boozer > Sent: Friday, December 11, 2009 8:08 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica vs Ventana > > > Anyone out there found an advantage of going from Ventana to Leica for > IHC? I am being warned about extra costs and protocol issues with > the 3 > trays. > > In negotiations.... > > > Kathy Boozer, HT (ASCP), IHCQ > Adventist Medical Center > 10123 SE Market St. > Portland, OR 97216 > boozerka@ah.org > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel Health > Systems, Alegent Health is faithful to the healing ministry of > Jesus Christ, providing high quality care for the body, mind and > spirit of every person. > > The information contained in this communication, including > attachments, is confidential and private and intended only for the > use of the addressees. Unauthorized use, disclosure, distribution > or copying is strictly prohibited and may be unlawful. If you > received this communication in error, please inform us of the > erroneous delivery by return e-mail message from your computer. > Additionally, although all attachments have been scanned at the > source for viruses, the recipient should check any attachments for > the presence of viruses before opening. Alegent Health accepts no > liability for any damage caused by any virus transmitted by this e- > mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Fri Dec 11 12:00:14 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Fri Dec 11 12:00:22 2009 Subject: [Histonet] out of office Message-ID: I will be out of the office starting 12/11/2009 and will not return until 12/14/2009. .In my absence please ask for Mary Campbell . If this is urgent you can contact me on my cell phone number 858-472-4266. From rsrichmond <@t> gmail.com Fri Dec 11 12:39:00 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Dec 11 12:39:04 2009 Subject: [Histonet] Re: alternates to absolute ethanol Message-ID: The commonly used (in medical histology anyway) alcohol is 90% ethanol, 5% methanol, and 5% isopropanol. Since it's denatured, you don't have the nuisance and taxes of handling beverage-quality ethanol. The common name for this mix is "reagent alcohol". Other denaturants such as methylisobutylketone (MIBK) (smells bad) and acetone (removes eosin) should be avoided. Bob Richmond Samurai Pathologist Knoxville TN From cmiller <@t> physlab.com Fri Dec 11 12:46:08 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Dec 11 12:46:14 2009 Subject: [Histonet] Injuries in the Lab In-Reply-To: <827428.56106.qm@web65713.mail.ac4.yahoo.com> References: <827428.56106.qm@web65713.mail.ac4.yahoo.com> Message-ID: We send them to an urgent care facility under workmans comp. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Friday, December 11, 2009 9:32 AM To: histonet Subject: [Histonet] Injuries in the Lab How are people dealing with injuries like cuts from the microtome blade or cuts at the grossing bench (fixed tissue only)? We do not work in a hospital so we do not have employee health. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From gayle.callis <@t> bresnan.net Fri Dec 11 12:55:20 2009 From: gayle.callis <@t> bresnan.net (gayle.callis@bresnan.net) Date: Fri Dec 11 12:54:54 2009 Subject: [Histonet] alternates to absolute ethanol In-Reply-To: <436000.23257.qm@web65705.mail.ac4.yahoo.com> References: <6B1915839B67AD46B88E6BE8F27376BB19FBCC@FMD3VS031.fda.gov><436000.23257.qm@web65705.mail.ac4.yahoo.com> Message-ID: <1384486449-1260557688-cardhu_decombobulator_blackberry.rim.net-1748529605-@bda420.bisx.prod.on.blackberry> We didn't find it cheaper either. Look for a reagent alcohol mixture that is mostly ethanol. There are many mixtures of this Gayle Callis Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Date: Fri, 11 Dec 2009 09:40:15 To: Histonet; Michael POwen Subject: RE: [Histonet] alternates to absolute ethanol The concern with methanol would be its toxicity. Methanol (TWA=200 ppm)?is 5 times more toxic than ethanol (TWA=1000 ppm) and twice more toxic than 2-propanol (TWA=400ppm). Ren? J. --- On Fri, 12/11/09, Owen, Michael P wrote: From: Owen, Michael P Subject: RE: [Histonet] alternates to absolute ethanol To: "Histonet" Date: Friday, December 11, 2009, 12:23 PM Dear Michael, Have you tried absolute methanol? The cost of the reagent should be less expensive than absolute ethanol and is easier to obtain than absolute ethanol (i.e. less legal restrictions than absolute ethanol that can be diverted to non-laboratory use). When fixing bacterial smears for a variety of staining methods, I have discovered absolute methanol works well and is often superior to heat fixation. Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE? Bothell, WA 98021-4421 Phone: 425-483-4865? ???E-Mail: michael.owen@fda.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Fri Dec 11 13:17:23 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Fri Dec 11 13:18:13 2009 Subject: [Histonet] Re: alternates to absolute ethanol In-Reply-To: References: Message-ID: American Mastertech has a Reagent Grade Ethyl Alcohol which is formulated with 95%, 200 proof ethyl alcohol and denatured with 5% isopropyl alcohol, NO Methanol! Cat #ALREACS for 4 Gal. It costs a little more, but well worth it! If you read the fine print on the contents of most of your histo and reagent grade alcohols, it is mostly denatured with Methanol. We would use this American Mastertech Reagent grade for our H&E, IHC and Special Stains, stain line. We also made ALL of our products with this grade while I was at Biocare. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Dec 11, 2009, at 11:39 AM, Robert Richmond wrote: > The commonly used (in medical histology anyway) alcohol is 90% > ethanol, 5% methanol, and 5% isopropanol. Since it's denatured, you > don't have the nuisance and taxes of handling beverage-quality > ethanol. The common name for this mix is "reagent alcohol". Other > denaturants such as methylisobutylketone (MIBK) (smells bad) and > acetone (removes eosin) should be avoided. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Fri Dec 11 13:28:39 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Dec 11 13:28:44 2009 Subject: [Histonet] Re: alternates to absolute ethanol In-Reply-To: References: Message-ID: Without the methanol, is this a legal denaturing formula in the USA? With the methanol, it's called S3D denatured alcohol. Bob Richmond On Fri, Dec 11, 2009 at 2:17 PM, Akemi Allison wrote: > American Mastertech has a?Reagent Grade Ethyl Alcohol which?is formulated > with 95%, 200 proof ethyl alcohol and denatured with 5% isopropyl alcohol, > NO Methanol! ?Cat #ALREACS for 4 Gal. ?It costs a little more, but well > worth it! If you read the fine print on the contents of most of your histo > and reagent grade alcohols, it is mostly denatured with Methanol. > We would use this American Mastertech Reagent ?grade for our H&E, IHC and > Special Stains, stain line. ?We also made ALL of our products with this > grade while I was at Biocare. > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele:?408.335.9994 > E-Mail: akemiat3377@yahoo.com > On Dec 11, 2009, at 11:39 AM, Robert Richmond wrote: > > The commonly used (in medical histology anyway) alcohol is 90% > ethanol, 5% methanol, and 5% isopropanol. Since it's denatured, you > don't have the nuisance and taxes of handling beverage-quality > ethanol. The common name for this mix is "reagent alcohol". Other > denaturants such as methylisobutylketone (MIBK) (smells bad) and > acetone (removes eosin) should be avoided. > Bob Richmond > Samurai Pathologist > Knoxville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akemiat3377 <@t> yahoo.com Fri Dec 11 13:39:16 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Fri Dec 11 13:40:06 2009 Subject: [Histonet] Re: alternates to absolute ethanol In-Reply-To: References: Message-ID: I have been purchasing this grade of alcohol for at least 12 years from American Mastertech. They are based in California, and California has some of the most stringent standards in the country. American Mastertech spent a fortune building their alcohol storage containers, as well as jumping ALL the hoops and hurdles for government approval. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Dec 11, 2009, at 12:28 PM, Robert Richmond wrote: > Without the methanol, is this a legal denaturing formula in the USA? > With the methanol, it's called S3D denatured alcohol. > > Bob Richmond > > On Fri, Dec 11, 2009 at 2:17 PM, Akemi Allison > wrote: >> American Mastertech has a Reagent Grade Ethyl Alcohol which is >> formulated >> with 95%, 200 proof ethyl alcohol and denatured with 5% isopropyl >> alcohol, >> NO Methanol! Cat #ALREACS for 4 Gal. It costs a little more, but >> well >> worth it! If you read the fine print on the contents of most of >> your histo >> and reagent grade alcohols, it is mostly denatured with Methanol. >> We would use this American Mastertech Reagent grade for our H&E, >> IHC and >> Special Stains, stain line. We also made ALL of our products with >> this >> grade while I was at Biocare. >> >> Akemi Allison BS, HT (ASCP) HTL >> Director >> Phoenix Lab Consulting >> Tele: 408.335.9994 >> E-Mail: akemiat3377@yahoo.com >> On Dec 11, 2009, at 11:39 AM, Robert Richmond wrote: >> >> The commonly used (in medical histology anyway) alcohol is 90% >> ethanol, 5% methanol, and 5% isopropanol. Since it's denatured, you >> don't have the nuisance and taxes of handling beverage-quality >> ethanol. The common name for this mix is "reagent alcohol". Other >> denaturants such as methylisobutylketone (MIBK) (smells bad) and >> acetone (removes eosin) should be avoided. >> Bob Richmond >> Samurai Pathologist >> Knoxville TN >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Fri Dec 11 15:17:06 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Dec 11 15:17:13 2009 Subject: [Histonet] Help with Large Eye Specimen Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46CD8@nmdamailsvr.nmda.ad.nmsu.edu> I do not have the equipment to do a whole horse eye (with levels) on "lantern" slides. We rarely have call for this and my boss would like to know if there is a vet school or reference lab that could process an entire eye, cut levels (to be determined) to lantern slides. Alternately, if you know a procedure for producing this kind of levels work that involves a regular 1x3" slide that I could do here, could you clue me in? The Boss would prefer the whole eye mount method if there is a facility that could perform this kind of work for us. Thanks in advance. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From b-frederick <@t> northwestern.edu Fri Dec 11 15:15:47 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Dec 11 15:18:24 2009 Subject: [Histonet] maximum block cutting In-Reply-To: <80542.74357.qm@web65701.mail.ac4.yahoo.com> Message-ID: We were told that to be efficient and highly productive (and this was in 1985) the quota was a block per minute. That way the work would be out on time. And yes, the quality had to be high. I had 3 months from the day I started work, fresh from histo school, to be at the pace and quality of techs with at lest 10 years experience. Cutting at least 200 slides was and to me was normal for the day. And still is. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, December 11, 2009 11:36 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu; histology@medsurgpath.com; Cathy; ToniRathborne; jellin@yumaregional.org Subject: Re: [Histonet] maximum block cutting It is to be published in January 2010. If you "accept" 24 blocks/hour = 2.5 min/block Ren? J. --- On Fri, 12/11/09, jellin@yumaregional.org wrote: From: jellin@yumaregional.org Subject: Re: [Histonet] maximum block cutting To: "Rene J Buesa" , histonet-bounces@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, "Cathy" , "ToniRathborne" Date: Friday, December 11, 2009, 12:10 PM When was this data gathered... Some of this sounds excessive like 2.5 minutes a block??? Are these based on complete manual process or is automation also taken into consideration.? Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Date: Fri, 11 Dec 2009 08:47:45 To: ; ; Cathy; ToniRathborne Subject: RE: [Histonet] maximum block cutting Cutting : average of 2.5 min/block ( = 24/hour) Trimming: average of 25 secs./block ( =144/hour) Ren? J. --- On Fri, 12/11/09, Rathborne, Toni wrote: From: Rathborne, Toni Subject: RE: [Histonet] maximum block cutting To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, "Cathy" Date: Friday, December 11, 2009, 9:03 AM So what would the time be for trimming and cutting? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 4:29 PM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com; Cathy Subject: RE: [Histonet] maximum block cutting Just actual cutting. Trimming is considered another task. Ren? J. --- On Thu, 12/10/09, Cathy wrote: From: Cathy Subject: RE: [Histonet] maximum block cutting To: "'Rene J Buesa'" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com Date: Thursday, December 10, 2009, 12:22 PM Does this average include trimming into the block or just the actual cutting of the section? Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com Subject: Re: [Histonet] maximum block cutting The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/hour?with an average of 24 (sample size = 245 histolabs). Ren? J. --- On Wed, 12/9/09, histology@medsurgpath.com wrote: From: histology@medsurgpath.com Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Dec 11 15:26:55 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 11 15:26:59 2009 Subject: [Histonet] maximum block cutting In-Reply-To: Message-ID: <489305.93428.qm@web65705.mail.ac4.yahoo.com> Cutting 1 block/minute is a possibility but there are some caveats like a perfect infiltration, a good simple tissue and only 1-2 sections/block. It is really very difficult to cut 60 blocks/hour when you have small biopsies or levels to cut. The average figure of 24 blocks/hour?refers to?a mixture of different types of tissues as in a general AP histolab. To the above you have to add that not all histotechs have the same dexterity; some are really slow and others fast. Not all histotechs "are?created equal", in the same way that not all blocks can be cut at the same speed. Not considering these facts and expecting that many blocks from every histotech could result in a below average quality and in mistakes especially "matching" the sections with the correct slide. Ren? J. --- On Fri, 12/11/09, Bernice Frederick wrote: From: Bernice Frederick Subject: RE: [Histonet] maximum block cutting To: "'Rene J Buesa'" , histonet-bounces@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, "'Cathy'" , "'ToniRathborne'" , jellin@yumaregional.org Date: Friday, December 11, 2009, 4:15 PM We were told that to be efficient and highly productive (and this was in 1985) the quota was a block per minute. That way the work would be out on time. And yes, the quality had to be high. I had 3 months from the day I started work, fresh from histo school, to be at the pace and quality of techs with at lest 10 years experience. Cutting at least 200 slides was and to me was normal for the day. And still is. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, December 11, 2009 11:36 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu; histology@medsurgpath.com; Cathy; ToniRathborne; jellin@yumaregional.org Subject: Re: [Histonet] maximum block cutting It is to be published in January 2010. If you "accept" 24 blocks/hour = 2.5 min/block Ren? J. --- On Fri, 12/11/09, jellin@yumaregional.org wrote: From: jellin@yumaregional.org Subject: Re: [Histonet] maximum block cutting To: "Rene J Buesa" , histonet-bounces@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, "Cathy" , "ToniRathborne" Date: Friday, December 11, 2009, 12:10 PM When was this data gathered... Some of this sounds excessive like 2.5 minutes a block??? Are these based on complete manual process or is automation also taken into consideration.? Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Date: Fri, 11 Dec 2009 08:47:45 To: ; ; Cathy; ToniRathborne Subject: RE: [Histonet] maximum block cutting Cutting : average of 2.5 min/block ( = 24/hour) Trimming: average of 25 secs./block ( =144/hour) Ren? J. --- On Fri, 12/11/09, Rathborne, Toni wrote: From: Rathborne, Toni Subject: RE: [Histonet] maximum block cutting To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, "Cathy" Date: Friday, December 11, 2009, 9:03 AM So what would the time be for trimming and cutting? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 4:29 PM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com; Cathy Subject: RE: [Histonet] maximum block cutting Just actual cutting. Trimming is considered another task. Ren? J. --- On Thu, 12/10/09, Cathy wrote: From: Cathy Subject: RE: [Histonet] maximum block cutting To: "'Rene J Buesa'" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com Date: Thursday, December 10, 2009, 12:22 PM Does this average include trimming into the block or just the actual cutting of the section? Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com Subject: Re: [Histonet] maximum block cutting The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/hour?with an average of 24 (sample size = 245 histolabs). Ren? J. --- On Wed, 12/9/09, histology@medsurgpath.com wrote: From: histology@medsurgpath.com Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Dec 11 15:29:41 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 11 15:29:46 2009 Subject: [Histonet] Help with Large Eye Specimen In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46CD8@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <973165.88148.qm@web65715.mail.ac4.yahoo.com> Hi Sara: I am forwarding your e-mail to J. Staruk because I think he can do that type of work. You can also consult him at the included e-mail address. Ren? J. --- On Fri, 12/11/09, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] Help with Large Eye Specimen To: histonet@lists.utsouthwestern.edu Date: Friday, December 11, 2009, 4:17 PM I do not have the equipment to do a whole horse eye (with levels) on "lantern" slides.? We rarely have call for this and my boss would like to know if there is a vet school or reference lab that could process an entire eye, cut levels (to be determined) to lantern slides. Alternately, if you know a procedure for producing this kind of levels work that involves a regular 1x3" slide that I could do here, could you clue me in?? The Boss would prefer the whole eye mount method if there is a facility that could perform this kind of work for us.? Thanks in advance. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Dec 11 16:19:14 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Dec 11 16:20:05 2009 Subject: SPAM-LOW: RE: [Histonet] Leica vs Ventana In-Reply-To: References: <7722595275A4DD4FA225B92CDBF174A1E8C9F7E017@EXC-MBX3.cfs.le.ac.uk><979FF5962E234F45B06CF0DB7C1AABB21B645E7F@chi2k3ms01.columbuschildrens.net><8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A54B6@EXCHMBC2.ad.ah.local> Message-ID: <64E062D1408F4121B1AEA5A442F406A5@Patsyoffice> We have both and each serves it's purpose, I like using the Leica Bond for research and find it to be a much more open system (with research version of the software, and you can switch back and forth from research and clincal on the same instrument, so the clinical work I do on the Bond is clinical research, not pure clinical, because right now I have my Bond in a non CAP lab, I could move it to the CAP lab and then use it for clinical work down there, I could still do research on it as well I believe, because for CAP you cannot report out clinical samples in a non CAP lab, but you can do as much research in a CAP clinical lab as you want, those rules only go that direction, research only in a non CAP lab, but a CAP certified lab can do research and clinical). Down stairs in the Derm Path lab they use just the Ventana Ultra and seem to be happy with that. As far as cost, each of them will tell you the other will cost more than theirs to run, I haven't had both of them long enough to say who is right but I amagine they will be comparable, each company does market research and knows what the market will bare and what they have to sell their stuff for to stay competitive the other, so I would be surprised if there was a huge huge difference in costs. For me it comes down to who you feel the most comfortable with, and who you trust, that is all I have to say about that. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karin Groeger Sent: Friday, December 11, 2009 8:49 AM To: Mahoney,Janice A; Houston, Ronald; Edwards,Richard E.; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] Leica vs Ventana We are a large Reference Lab and use both instruments and get good results and vendor support from Leica and Ventana, if you are a small lab then it should be your preference and what you feel comfortable with. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Friday, December 11, 2009 7:02 AM To: 'Houston, Ronald'; Edwards,Richard E.; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana I think these obscure emails about vendors are very damaging, regardless of who the vender is. If someone is experiencing a particular problem with an instrument, please use this venue to get advice on how others handled it. Be open about the problem so that we all can learn from your experiences. To dog a company without any real information is not useful to anyone. Both companies have good instruments that may suit different types of labs better than the other. I'll get off my soap box now. Jan Mahoney, Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Friday, December 11, 2009 8:55 AM To: Edwards, Richard E.; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Probably depends on whether you're talking about a white elephant or not Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: Edwards, Richard E. [mailto:ree3@leicester.ac.uk] Sent: Friday, December 11, 2009 9:52 AM To: Houston, Ronald; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana This sounds like the game children play, so who would win the fight between an elephant and a hippopotamus, or the fight between a banana and a pomegranate etcetc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: 11 December 2009 14:44 To: Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Not enough time in the day to answer that one Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, December 11, 2009 9:41 AM To: Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Why would you switch from a reliable, walk-away, reproducible system? Just curious. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Friday, December 11, 2009 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica vs Ventana Anyone out there found an advantage of going from Ventana to Leica for IHC? I am being warned about extra costs and protocol issues with the 3 trays. In negotiations.... Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. 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The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Dec 11 16:20:43 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Dec 11 16:21:28 2009 Subject: SPAM-LOW: [Histonet] Leica vs Ventana In-Reply-To: <4B21E1C5.4AA8.00C0.0@ah.org> References: <4B21E1C5.4AA8.00C0.0@ah.org> Message-ID: <80D9EC84BFB649C2AC871FEBBD6E3B53@Patsyoffice> Kathleen, You can contact me privately and discuss this further. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Friday, December 11, 2009 7:08 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Leica vs Ventana Anyone out there found an advantage of going from Ventana to Leica for IHC? I am being warned about extra costs and protocol issues with the 3 trays. In negotiations.... Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Dec 11 17:01:23 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Dec 11 17:01:32 2009 Subject: [Histonet] This may be a repeat, with additions Re: Leica vs Ventana Message-ID: <000001ca7ab5$dda41df0$98ec59d0$@callis@bresnan.net> Well said ladies, and thank you! Gayle Callis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Friday, December 11, 2009 3:19 PM To: 'Karin Groeger'; 'Mahoney,Janice A'; 'Houston, Ronald'; 'Edwards,Richard E.'; 'Sebree Linda A'; 'Kathleen Boozer'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: RE: [Histonet] Leica vs Ventana We have both and each serves it's purpose, I like using the Leica Bond for research and find it to be a much more open system (with research version of the software, and you can switch back and forth from research and clincal on the same instrument, so the clinical work I do on the Bond is clinical research, not pure clinical, because right now I have my Bond in a non CAP lab, I could move it to the CAP lab and then use it for clinical work down there, I could still do research on it as well I believe, because for CAP you cannot report out clinical samples in a non CAP lab, but you can do as much research in a CAP clinical lab as you want, those rules only go that direction, research only in a non CAP lab, but a CAP certified lab can do research and clinical). Down stairs in the Derm Path lab they use just the Ventana Ultra and seem to be happy with that. As far as cost, each of them will tell you the other will cost more than theirs to run, I haven't had both of them long enough to say who is right but I amagine they will be comparable, each company does market research and knows what the market will bare and what they have to sell their stuff for to stay competitive the other, so I would be surprised if there was a huge huge difference in costs. For me it comes down to who you feel the most comfortable with, and who you trust, that is all I have to say about that. Regards, Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net **************************************************************************** **************************************************************************** ************************ We are a large Reference Lab and use both instruments and get good results and vendor support from Leica and Ventana, if you are a small lab then it should be your preference and what you feel comfortable with. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 From a_jimmy11 <@t> yahoo.com Fri Dec 11 17:04:31 2009 From: a_jimmy11 <@t> yahoo.com (Jimmy A) Date: Fri Dec 11 17:04:34 2009 Subject: [Histonet] VENTANA AUTOSTAINERS PLS Message-ID: <697063.33257.qm@web112813.mail.gq1.yahoo.com> ? ????? Hi, ? ? I am looking for a lab that can allow me to spend one or two days to properly see how the ventana autostainer?works. ???? I am an histotech working here?in the US. I am interested in learning? the operation of the ventana immunostainers. I will appreciate it, if one of the?histology/ihc labs could?grant me?this great favour. ???? ?Hoping to hear from you guys asap. ? ???? Jimmy. From tjasper <@t> copc.net Fri Dec 11 17:25:23 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri Dec 11 17:25:29 2009 Subject: FW: [Histonet] VENTANA AUTOSTAINERS PLS Message-ID: <90354A475B420441B2A0396E5008D496731A50@copc-sbs.COPC.local> One more time! -----Original Message----- From: Thomas Jasper Sent: Friday, December 11, 2009 3:24 PM To: 'Jimmy A' Subject: RE: [Histonet] VENTANA AUTOSTAINERS PLS Jimmy, What the...!!? I don't think anyone is going to be able to help you. This is a vague request and I'm beginning to wonder about the sincerity and credibility of it. My suggestion - Either get real or please get off this list and leave folks be. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jimmy A Sent: Friday, December 11, 2009 3:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VENTANA AUTOSTAINERS PLS ? ????? Hi, ? ? I am looking for a lab that can allow me to spend one or two days to properly see how the ventana autostainer?works. ???? I am an histotech working here?in the US. I am interested in learning? the operation of the ventana immunostainers. I will appreciate it, if one of the?histology/ihc labs could?grant me?this great favour. ???? ?Hoping to hear from you guys asap. ? ???? Jimmy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat Dec 12 12:43:53 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat Dec 12 12:43:57 2009 Subject: [Histonet] Re: Help with Large Eye Specimen Message-ID: Sally Breeden, HT(ASCP) at the NM Dept. of Agriculture Veterinary Diagnostic Services in Albuquerque asks: >>I do not have the equipment to do a whole horse eye (with levels) on "lantern" slides. We rarely have call for this and my boss would like to know if there is a vet school or reference lab that could process an entire eye, cut levels (to be determined) to lantern slides. Alternately, if you know a procedure for producing this kind of levels work that involves a regular 1x3" slide that I could do here, could you clue me in? The Boss would prefer the whole eye mount method if there is a facility that could perform this kind of work for us.<< My friend Dick Dubielzig (pronounced dooBILLzig), a veterinary pathologist specializing in veterinary ophthalmology, can probably help you with this. See http://www.vetmed.wisc.edu/people/dubielzr Bob Richmond Samurai Pathologist Knoxville TN From gayle.callis <@t> bresnan.net Sat Dec 12 14:54:31 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Sat Dec 12 14:54:41 2009 Subject: [Histonet] Using acetic acid alone as a fixative Message-ID: <000001ca7b6d$4eafdb80$ec0f9280$@callis@bresnan.net> Dear Histonetters, I have a person using acetic acid as a fixative for frozen sections containing bioluminescent proteins (GFP and tdTomato, a red fluorescent protein). I have always been taught to use acetic acid in combination with other chemical agents (e.g. as in Bouins or Carnoys) but not alone due to the swelling of cells and tissues and probably other acid protein hydrolysis going on too. Has anyone ever run across using acetic acid (concentration is not high, but not known by me)? She does encounter problems with GFP staining, but the tdTomato still glows although her results are inconsistent. I have no clue where this fixation method originated from but I suspect it may be from a fixative where something was lost in making it up/forgotten/left out - ending up with acetic acid alone. A quick look in Sheehan and Hrapchak's Theory and Practice of HIstotechnology, indicated acetic acid was rarely used alone for fixation. I am off to John Kiernan's book after sending this message. I understand the sensitivity of GFP and some other GFP chimeras , also RFP (closely related structurally to GFP) to alcohols and other organic solvents, heat, and pH where fluorescence is lost and/or diminished significantly. Enlighten me please Gayle M. Callis HTL/HT/MT(ASCP) From sandoval.1965 <@t> hotmail.com Sat Dec 12 15:00:37 2009 From: sandoval.1965 <@t> hotmail.com (Anthony Sandoval) Date: Sat Dec 12 15:00:15 2009 Subject: [Histonet] basic IHC guide Message-ID: Hello Histonet, I am pretty new to IHC and would like to study a basic text book. Can anyone give me a recommendation? Thank you for the support! From liz <@t> premierlab.com Sat Dec 12 15:07:47 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Sat Dec 12 15:07:50 2009 Subject: [Histonet] basic IHC guide In-Reply-To: Message-ID: Get the book from Dako - Its called Immunohistochemical Staining Methods - 4th edition. It's a great reference and its free. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony Sandoval Sent: Saturday, December 12, 2009 2:01 PM To: histo net Subject: [Histonet] basic IHC guide Hello Histonet, I am pretty new to IHC and would like to study a basic text book. Can anyone give me a recommendation? Thank you for the support! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Dec 12 15:09:07 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Dec 12 15:09:11 2009 Subject: [Histonet] basic IHC guide In-Reply-To: Message-ID: <702568.69116.qm@web65704.mail.ac4.yahoo.com> Contact DAKO because they have a IHC guide available that is pretty good. Ren? J, --- On Sat, 12/12/09, Anthony Sandoval wrote: From: Anthony Sandoval Subject: [Histonet] basic IHC guide To: "histo net" Date: Saturday, December 12, 2009, 4:00 PM Hello Histonet, I am pretty new to IHC and would like to study a basic text book. Can anyone give me a recommendation? Thank you for the support! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Sat Dec 12 22:30:26 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Dec 12 22:30:30 2009 Subject: [Histonet] basic IHC guide Message-ID: Dear Anthony Sandoval, It's a breath of fresh air to see someone using Histonet to ask about a book rather than ask for a "protocol". For simple but thorough explanations and easily followed instructions I strongly recommend Polak, J.M., and Van Noorden, S. (1997) Royal Microscopical Society Microscopy Handbooks, 37: Introduction to Immunocytochemistry, 2nd ed. Oxford: BIOS Scientific Publications. There is a 3rd edition (2003) that I don't have. It sells for US$61.24 on Amazon.com: http://www.amazon.com/Introduction-Immunocytochemistry-J-M-Polak/dp/1859962084 The 2nd edition, 160-page paperback, cost me 12 pounds (about 20 US$) about 10 years ago. There will be second-hand copies on the market, but I'm not selling mine. Despite its title, this is a book about immunohistochemistry! (This paragraph is for those of us who don't like "immunocytochemistry" or think the word should be reserved for methods applied to cell cultures, smears of exfoliated cells, blood films, cytocentrifuge preps etc.) Susan Van Noorden also wrote an excellent chapter about immunohistochemistry in a Biochemical Society publication (2002): Microscopy and Histology for Molecular Biologists: A User's Guide. London: Portland Press. - ISBN 1855781417 [hard cover]; ISBN 1855781565 [paperback] http://www.portlandpress.com/pp/books/search/searchres.htm?SearchTerm=histology Unfortunately, this is a rather expensive book (50 pounds for the paperback or 110 pounds for the hardback). The Biochemical Society's market is labs (paperback) and libraries (hardcover), rather than individuals. SVN's chapter epitomizes the content the book she co-authored with Julia Polak. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Anthony Sandoval Date: Saturday, December 12, 2009 16:00 Subject: [Histonet] basic IHC guide To: histo net > Hello Histonet, > > I am pretty new to IHC and would like to study a basic text > book. Can anyone > give me a recommendation? Thank you for the support! > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Sat Dec 12 22:50:35 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Dec 12 22:50:39 2009 Subject: [Histonet] fish scales In-Reply-To: <825755352-1260478149-cardhu_decombobulator_blackberry.rim.net-1012507121-@bda420.bisx.prod.on.blackberry> References: <825755352-1260478149-cardhu_decombobulator_blackberry.rim.net-1012507121-@bda420.bisx.prod.on.blackberry> Message-ID: Fish scales are calcified. Depending on the type of fish, they may contain dentin, bone or both. My sources: Two zoology textbooks and also a Wikipedia entry, Scales (zoology) that reads easily. Even boneless fishes like sharks have calcified teeth and skin. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: gayle.callis@bresnan.net Date: Thursday, December 10, 2009 15:50 Subject: Re: [Histonet] fish scales To: "Perry, Margaret" , histonet-bounces@lists.utsouthwestern.edu, "histonet@lists.utsouthwestern.edu" > Are fish scales even calcified? If so decalcifying may not work. > If keratin - softening with ammonia water, Mollifex(sp?), Nair > which is alkaline. I would be more tempted to use GMA plastic to > match hardness of scales. > I have a protocol for horse hooves. If you want will send > privately. This is also in HistoLogic, author is Jane > Chladny. > Gayle Callis > Sent from my Verizon Wireless BlackBerry > > -----Original Message----- > From: "Perry, Margaret" > Date: Thu, 10 Dec 2009 12:32:09 > To: > histonet@lists.utsouthwestern.eduSubject: [Histonet] fish scales > > We are trying to cut fish scales that have been > decalcified. They are chunking out when we try to cut > them. I think we need to soften the keratin and I looked > in the archives for the right dilution of ammonium hydroxide to > use. One post said 5% the other said straight. What > dilution do you recommend? I'm think it would probably be > the same as what you use on toenails. > > Margaret Perry HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Sun Dec 13 10:28:52 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Sun Dec 13 10:29:06 2009 Subject: [Histonet] fish scales and horse hooves (softening) In-Reply-To: References: <825755352-1260478149-cardhu_decombobulator_blackberry.rim.net-1012507121-@bda420.bisx.prod.on.blackberry> Message-ID: <000601ca7c11$5daf2c90$190d85b0$@callis@bresnan.net> Thank you John. I did a search and found a excellent explanation on different types of fish scales. Having not worked with the species nor their scales, I am now enlightened on scales. As for Margaret's problem, Jane Chladny had a protocol for horse hooves although she used very long processing schedule for these large specimens, and I did not include that information in this message. However, Margaret may want to try this method , a combination softening and decalcification. Jane also published this method with more discussion in Sakura's HistoLogic (check their website archives). Margaret can adjust her times in the following solution to fit her needs and size of scales. Jane Chladny Horse Hoof Fixation/Decalcification/Softening. After complete fixation, we soften the hoof for 1-2 weeks ( adjust time to fit fish scales) in a solution of 10 ml Tween 80 in 100 ml 1N HCl. Following softening, the tissue is rinsed in water and processed. Happy Holidays Gayle M. Callis HTL/HT/MT(ASCP) From: John Kiernan [mailto:jkiernan@uwo.ca] Sent: Saturday, December 12, 2009 9:51 PM To: gayle.callis@bresnan.net Cc: Perry, Margaret; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] fish scales Fish scales are calcified. Depending on the type of fish, they may contain dentin, bone or both. My sources: Two zoology textbooks and also a Wikipedia entry, Scales (zoology) that reads easily. Even boneless fishes like sharks have calcified teeth and skin. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: gayle.callis@bresnan.net Date: Thursday, December 10, 2009 15:50 Subject: Re: [Histonet] fish scales To: "Perry, Margaret" , histonet-bounces@lists.utsouthwestern.edu, "histonet@lists.utsouthwestern.edu" > Are fish scales even calcified? If so decalcifying may not work. > If keratin - softening with ammonia water, Mollifex(sp?), Nair > which is alkaline. I would be more tempted to use GMA plastic to > match hardness of scales. > I have a protocol for horse hooves. If you want will send > privately. This is also in HistoLogic, author is Jane > Chladny. > Gayle Callis > Sent from my Verizon Wireless BlackBerry > > -----Original Message----- > From: "Perry, Margaret" > Date: Thu, 10 Dec 2009 12:32:09 > To: > histonet@lists.utsouthwestern.eduSubject: [Histonet] fish scales > > We are trying to cut fish scales that have been > decalcified. They are chunking out when we try to cut > them. I think we need to soften the keratin and I looked > in the archives for the right dilution of ammonium hydroxide to > use. One post said 5% the other said straight. What > dilution do you recommend? I'm think it would probably be > the same as what you use on toenails. > > Margaret Perry HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Dec 13 12:26:06 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Dec 13 12:26:51 2009 Subject: SPAM-LOW: [Histonet] basic IHC guide In-Reply-To: References: Message-ID: Anthony, You might want to join the NSH IHC Resource Group at www.ihcrg.org online, we are a group of over 500 from all over the world working in IHC. The website, same as where you register has lists of references to use in IHC and a workshop presented by Ethel Macrea on taking the ASCP QIHC exam. The best part is that this resource group has it's own list server like Histonet that focuses just on IHC subjects. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony Sandoval Sent: Saturday, December 12, 2009 2:01 PM To: histo net Subject: SPAM-LOW: [Histonet] basic IHC guide Hello Histonet, I am pretty new to IHC and would like to study a basic text book. Can anyone give me a recommendation? Thank you for the support! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Dec 13 12:28:59 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Dec 13 12:29:45 2009 Subject: SPAM-LOW: [Histonet] VENTANA AUTOSTAINERS PLS In-Reply-To: <697063.33257.qm@web112813.mail.gq1.yahoo.com> References: <697063.33257.qm@web112813.mail.gq1.yahoo.com> Message-ID: <40A491E5AF444C94B043537CF2868E87@prueggihctechlt> Why don't you talk to Ventana about this, I am sure they would arrange something for you in hopes of getting your business. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jimmy A Sent: Friday, December 11, 2009 4:05 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] VENTANA AUTOSTAINERS PLS ? ????? Hi, ? ? I am looking for a lab that can allow me to spend one or two days to properly see how the ventana autostainer?works. ???? I am an histotech working here?in the US. I am interested in learning? the operation of the ventana immunostainers. I will appreciate it, if one of the?histology/ihc labs could?grant me?this great favour. ???? ?Hoping to hear from you guys asap. ? ???? Jimmy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Dec 13 12:33:14 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Dec 13 12:34:00 2009 Subject: SPAM-LOW: Re: [Histonet] Re: alternates to absolute ethanol In-Reply-To: References: Message-ID: <55EB40EF93EB479C87BAD72857886AAA@prueggihctechlt> I buy alcohols from Stat Labs and it seems to be the exact same formula, ethyl with isopropyl, no methanol, it may be the same manufacturer just a different distributer??= Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Friday, December 11, 2009 12:39 PM To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] Re: alternates to absolute ethanol I have been purchasing this grade of alcohol for at least 12 years from American Mastertech. They are based in California, and California has some of the most stringent standards in the country. American Mastertech spent a fortune building their alcohol storage containers, as well as jumping ALL the hoops and hurdles for government approval. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Dec 11, 2009, at 12:28 PM, Robert Richmond wrote: > Without the methanol, is this a legal denaturing formula in the USA? > With the methanol, it's called S3D denatured alcohol. > > Bob Richmond > > On Fri, Dec 11, 2009 at 2:17 PM, Akemi Allison > wrote: >> American Mastertech has a Reagent Grade Ethyl Alcohol which is >> formulated >> with 95%, 200 proof ethyl alcohol and denatured with 5% isopropyl >> alcohol, >> NO Methanol! Cat #ALREACS for 4 Gal. It costs a little more, but >> well >> worth it! If you read the fine print on the contents of most of >> your histo >> and reagent grade alcohols, it is mostly denatured with Methanol. >> We would use this American Mastertech Reagent grade for our H&E, >> IHC and >> Special Stains, stain line. We also made ALL of our products with >> this >> grade while I was at Biocare. >> >> Akemi Allison BS, HT (ASCP) HTL >> Director >> Phoenix Lab Consulting >> Tele: 408.335.9994 >> E-Mail: akemiat3377@yahoo.com >> On Dec 11, 2009, at 11:39 AM, Robert Richmond wrote: >> >> The commonly used (in medical histology anyway) alcohol is 90% >> ethanol, 5% methanol, and 5% isopropanol. Since it's denatured, you >> don't have the nuisance and taxes of handling beverage-quality >> ethanol. The common name for this mix is "reagent alcohol". Other >> denaturants such as methylisobutylketone (MIBK) (smells bad) and >> acetone (removes eosin) should be avoided. >> Bob Richmond >> Samurai Pathologist >> Knoxville TN >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhewlett <@t> cogeco.ca Sun Dec 13 13:02:39 2009 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Sun Dec 13 13:02:48 2009 Subject: [Histonet] Using acetic acid alone as a fixative References: <000001ca7b6d$4eafdb80$ec0f9280$@callis@bresnan.net> Message-ID: <25CD61D5842447AF95A0E3654297E431@mainbox> Hi Gayle, You are generally correct. Essentially, the only proteins denatured (fixed) by acetic acid are the nucleoproteins. However, 5% aqueous acetic acid has been used as a fixative for chromosomes in fresh material (commonly plant). We used this in school to see mitosis in onion root tip, how's that for a six decade old memory? I have also used this more recently (only four decades back) to fix cell cultures prior to staining for karyotype analysis. Today most labs use acetic/methanol for this purpose. The methods that I am familiar with, use either aceto-carmine or aceto-orcein as a combination fixative/stain for chromatin. I am sure that John K. can also come up with a version for Nissl substance. All the best for the season. Cheers, Bryan ----- Original Message ----- From: "gayle callis" To: "Histonet" Sent: Saturday, December 12, 2009 3:54 PM Subject: [Histonet] Using acetic acid alone as a fixative > Dear Histonetters, > > > > I have a person using acetic acid as a fixative for frozen sections > containing bioluminescent proteins (GFP and tdTomato, a red fluorescent > protein). > > > > I have always been taught to use acetic acid in combination with other > chemical agents (e.g. as in Bouins or Carnoys) but not alone due to the > swelling of cells and tissues and probably other acid protein hydrolysis > going on too. > > > > Has anyone ever run across using acetic acid (concentration is not high, > but > not known by me)? She does encounter problems with GFP staining, but the > tdTomato still glows although her results are inconsistent. > > > > I have no clue where this fixation method originated from but I suspect it > may be from a fixative where something was lost in making it > up/forgotten/left out - ending up with acetic acid alone. A quick look in > Sheehan and Hrapchak's Theory and Practice of HIstotechnology, indicated > acetic acid was rarely used alone for fixation. I am off to John > Kiernan's > book after sending this message. > > > > I understand the sensitivity of GFP and some other GFP chimeras , also RFP > (closely related structurally to GFP) to alcohols and other organic > solvents, heat, and pH where fluorescence is lost and/or diminished > significantly. > > > > Enlighten me please > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jphistology <@t> gmail.com Sun Dec 13 23:02:35 2009 From: jphistology <@t> gmail.com (Joao Pessoa) Date: Sun Dec 13 23:02:40 2009 Subject: [Histonet] Array Science Message-ID: <9e4d12d0912132102k7cc18df6k4ea814d893aa0274@mail.gmail.com> Have you people heard the company Array Science? I know from my friends they make tissue arrays for many years. Now I can not find information for them, though. I need tissue array slides from them. Thank you! Joao Histo Tech From jphistology <@t> gmail.com Sun Dec 13 23:20:01 2009 From: jphistology <@t> gmail.com (Joao Pessoa) Date: Sun Dec 13 23:20:05 2009 Subject: [Histonet] basic IHC guide Message-ID: <9e4d12d0912132120p78b40654j87d2b4019dce725c@mail.gmail.com> Dear Anthony, You should see 5th edition of the Dako IHC book. Welcome to exciting world of IHC! The URL is here: http://pri.dako.com/08002_ihc_staining_methods_5ed.pdf Joao Histo Tech From BoozerKA <@t> ah.org Mon Dec 14 06:19:58 2009 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Mon Dec 14 06:20:23 2009 Subject: [Histonet] Leica vs Ventana In-Reply-To: <227D3462-0D2D-4971-A0A8-34E5585839B3@yahoo.com> References: <7722595275A4DD4FA225B92CDBF174A1E8C9F7E017@EXC-MBX3.cfs.le.ac.uk> <979FF5962E234F45B06CF0DB7C1AABB21B645E7F@chi2k3ms01.columbuschildrens.net> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A54B6@EXCHMBC2.ad.ah.local> <227D3462-0D2D-4971-A0A8-34E5585839B3@yahoo.com> Message-ID: <4B25BCEC.4AA8.00C0.0@ah.org> My sincere apologies to all that I have offended. I asked a simple question and got a very helpful reply from someone who asked me to call them, as they had compared for their facility and it answered my questions. Kathy >>> Akemi Allison 12/11/2009 09:53 >>> Hi Janice, I'm glad you brought up your point! It's amazing how people are. It just shows how narrow minded and unkind in their statements. Yes, Ventana is HUGE, but they have their faults as well! That being said, there is always room to build a better mouse trap and save money while doing it too! The new Bond is amazing and is capable of doing multiple IHC staining on (1) slide. It is also a open system. "Science never sleeps"! Thank you for speaking out, and hope you have a Wonderful Holiday Season! Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Dec 11, 2009, at 8:02 AM, Mahoney,Janice A wrote: > I think these obscure emails about vendors are very damaging, > regardless of who the vender is. If someone is experiencing a > particular problem with an instrument, please use this venue to get > advice on how others handled it. Be open about the problem so that > we all can learn from your experiences. To dog a company without > any real information is not useful to anyone. > Both companies have good instruments that may suit different types > of labs better than the other. > I'll get off my soap box now. > Jan Mahoney, Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald > Sent: Friday, December 11, 2009 8:55 AM > To: Edwards, Richard E.; Sebree Linda A; Kathleen Boozer; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > Probably depends on whether you're talking about a white elephant > or not > > Ronnie Houston > Anatomic Pathology Manager > Nationwide Children's Hospital > Columbus OH 43205 > (614) 722 5450 > > -----Original Message----- > From: Edwards, Richard E. [mailto:ree3@leicester.ac.uk] > Sent: Friday, December 11, 2009 9:52 AM > To: Houston, Ronald; Sebree Linda A; Kathleen Boozer; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > This sounds like the game children play, so who would win the fight > between an elephant and a hippopotamus, or the fight between a banana > and a pomegranate etcetc. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Houston, > Ronald > Sent: 11 December 2009 14:44 > To: Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > Not enough time in the day to answer that one > > Ronnie Houston > Anatomic Pathology Manager > Nationwide Children's Hospital > Columbus OH 43205 > (614) 722 5450 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree > Linda A > Sent: Friday, December 11, 2009 9:41 AM > To: Kathleen Boozer; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > Why would you switch from a reliable, walk-away, reproducible system? > > Just curious. > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Kathleen > Boozer > Sent: Friday, December 11, 2009 8:08 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica vs Ventana > > > Anyone out there found an advantage of going from Ventana to Leica for > IHC? I am being warned about extra costs and protocol issues with > the 3 > trays. > > In negotiations.... > > > Kathy Boozer, HT (ASCP), IHCQ > Adventist Medical Center > 10123 SE Market St. > Portland, OR 97216 > boozerka@ah.org > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel Health > Systems, Alegent Health is faithful to the healing ministry of > Jesus Christ, providing high quality care for the body, mind and > spirit of every person. > > The information contained in this communication, including > attachments, is confidential and private and intended only for the > use of the addressees. Unauthorized use, disclosure, distribution > or copying is strictly prohibited and may be unlawful. If you > received this communication in error, please inform us of the > erroneous delivery by return e-mail message from your computer. > Additionally, although all attachments have been scanned at the > source for viruses, the recipient should check any attachments for > the presence of viruses before opening. Alegent Health accepts no > liability for any damage caused by any virus transmitted by this e- > mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From farnana <@t> nehealth.com Mon Dec 14 06:56:45 2009 From: farnana <@t> nehealth.com (Amy Farnan) Date: Mon Dec 14 06:57:03 2009 Subject: [Histonet] Re: Histonet Digest, Vol 73, Issue 18 In-Reply-To: <983422B4.936@neh_domain_app_srv.nehealth.com> References: <983422B4.936@neh_domain_app_srv.nehealth.com> Message-ID: <4B25EFBC.26E9.00D9.1@nehealth.com> I have a Leica Bond and I have had a couple of small issues but I think that is par for the course with any instrument. Nothing is full proof. I run a small lab that processes 20,000 surgicals a year. The Bond seemed more economical for my situation without compromising quality. I have had the instrument for 1.5 years and I would purchase another. >>> 12/11/2009 1:05 pm >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: fish scales (Josie Britton) 2. RE: Leica vs Ventana (Karin Groeger) 3. alternates to absolute ethanol (Michael W. Folsom) 4. RE: maximum block cutting (Rene J Buesa) 5. Re: alternates to absolute ethanol (Rene J Buesa) 6. one more question about reagent alcohol (Michael W. Folsom) 7. RE: alternates to absolute ethanol (Owen, Michael P) 8. Re: maximum block cutting (Rene J Buesa) 9. RE: alternates to absolute ethanol (Rene J Buesa) 10. Re: maximum block cutting (jellin@yumaregional.org) 11. Re: Leica vs Ventana (Akemi Allison) ---------------------------------------------------------------------- Message: 1 Date: Fri, 11 Dec 2009 10:34:46 -0500 From: "Josie Britton" Subject: RE: [Histonet] fish scales To: "Josie Britton" , "Perry, Margaret" , Message-ID: Content-Type: text/plain; charset="us-ascii" Oh, I forgot to mention we do this after we face the block. We put a little swirl on the block face and let it soak for 10-30+ minutes depending on spec. You can usually get a ribbon, then you have to re-soak for another ribbon. I hope this helps! Josie Britton HT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Josie Britton Sent: Friday, December 11, 2009 10:31 AM To: Perry, Margaret; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] fish scales Nair hair remover works great on toenails! Josie Britton HT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Thursday, December 10, 2009 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fish scales We are trying to cut fish scales that have been decalcified. They are chunking out when we try to cut them. I think we need to soften the keratin and I looked in the archives for the right dilution of ammonium hydroxide to use. One post said 5% the other said straight. What dilution do you recommend? I'm think it would probably be the same as what you use on toenails. Margaret Perry HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Fri, 11 Dec 2009 07:48:36 -0800 From: "Karin Groeger" Subject: RE: [Histonet] Leica vs Ventana To: "Mahoney,Janice A" , "Houston, Ronald" , "Edwards,Richard E." , "Sebree Linda A" , "Kathleen Boozer" , Message-ID: Content-Type: text/plain; charset="us-ascii" We are a large Reference Lab and use both instruments and get good results and vendor support from Leica and Ventana, if you are a small lab then it should be your preference and what you feel comfortable with. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Friday, December 11, 2009 7:02 AM To: 'Houston, Ronald'; Edwards,Richard E.; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana I think these obscure emails about vendors are very damaging, regardless of who the vender is. If someone is experiencing a particular problem with an instrument, please use this venue to get advice on how others handled it. Be open about the problem so that we all can learn from your experiences. To dog a company without any real information is not useful to anyone. Both companies have good instruments that may suit different types of labs better than the other. I'll get off my soap box now. Jan Mahoney, Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Friday, December 11, 2009 8:55 AM To: Edwards, Richard E.; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Probably depends on whether you're talking about a white elephant or not Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: Edwards, Richard E. [mailto:ree3@leicester.ac.uk] Sent: Friday, December 11, 2009 9:52 AM To: Houston, Ronald; Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana This sounds like the game children play, so who would win the fight between an elephant and a hippopotamus, or the fight between a banana and a pomegranate etcetc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: 11 December 2009 14:44 To: Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Not enough time in the day to answer that one Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, December 11, 2009 9:41 AM To: Kathleen Boozer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica vs Ventana Why would you switch from a reliable, walk-away, reproducible system? Just curious. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Friday, December 11, 2009 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica vs Ventana Anyone out there found an advantage of going from Ventana to Leica for IHC? I am being warned about extra costs and protocol issues with the 3 trays. In negotiations.... Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center 10123 SE Market St. Portland, OR 97216 boozerka@ah.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. ------------------------------ Message: 3 Date: Fri, 11 Dec 2009 09:05:04 -0700 From: "Michael W. Folsom" Subject: [Histonet] alternates to absolute ethanol To: histonet@lists.utsouthwestern.edu Message-ID: <4B226DB0.5090300@swcp.com> Content-Type: text/plain; charset=ISO-8859-1 Hi: I want to process some plant tissue for microtomy. When I was doing this many years ago the protocol I used involved absolute ethanol. Knowing that getting ethanol that is really anhydrous is hard I was wondering if folks were using stuff that was denatured with 55% absolute methanol and 5% isoproponol instead. I understand it has better shelf life but wondered about the addition of small mounts of methanol and isopropanol. Also, if anybody is using this can you recommend a supplier? Thanks - Mike Rio Grand Biological ------------------------------ Message: 4 Date: Fri, 11 Dec 2009 08:47:45 -0800 (PST) From: Rene J Buesa Subject: RE: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, Cathy , ToniRathborne Message-ID: <190590.2820.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Cutting : average of 2.5 min/block ( = 24/hour) Trimming: average of 25 secs./block ( =144/hour) Ren? J. --- On Fri, 12/11/09, Rathborne, Toni wrote: From: Rathborne, Toni Subject: RE: [Histonet] maximum block cutting To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, "Cathy" Date: Friday, December 11, 2009, 9:03 AM So what would the time be for trimming and cutting? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 4:29 PM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com; Cathy Subject: RE: [Histonet] maximum block cutting Just actual cutting. Trimming is considered another task. Ren? J. --- On Thu, 12/10/09, Cathy wrote: From: Cathy Subject: RE: [Histonet] maximum block cutting To: "'Rene J Buesa'" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com Date: Thursday, December 10, 2009, 12:22 PM Does this average include trimming into the block or just the actual cutting of the section? Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com Subject: Re: [Histonet] maximum block cutting The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/hour?with an average of 24 (sample size = 245 histolabs). Ren? J. --- On Wed, 12/9/09, histology@medsurgpath.com wrote: From: histology@medsurgpath.com Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. 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Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr ------------------------------ Message: 5 Date: Fri, 11 Dec 2009 09:03:10 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] alternates to absolute ethanol To: histonet@lists.utsouthwestern.edu, "Michael W. Folsom" Message-ID: <141761.25901.qm@web65715.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Pure isopropyl = isopropanol = 2-propanol is a better dehydrant than ethanol. Try it. Ren? J. --- On Fri, 12/11/09, Michael W. Folsom wrote: From: Michael W. Folsom Subject: [Histonet] alternates to absolute ethanol To: histonet@lists.utsouthwestern.edu Date: Friday, December 11, 2009, 11:05 AM Hi: I want to process some plant tissue for microtomy.? When I was doing this many years ago the protocol I used involved absolute ethanol. Knowing that getting ethanol that is really anhydrous is hard I was wondering if folks were using stuff that was denatured with 55% absolute methanol and 5% isoproponol instead.? I understand it has better shelf life but wondered about the addition of small mounts of methanol and isopropanol. Also, if anybody is using this can you recommend a supplier? Thanks - Mike Rio Grand Biological _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 11 Dec 2009 10:14:21 -0700 From: "Michael W. Folsom" Subject: [Histonet] one more question about reagent alcohol To: Histonet@lists.utsouthwestern.edu Message-ID: <4B227DED.6030404@swcp.com> Content-Type: text/plain; charset=ISO-8859-1 Folks: Thanks for the many quick responses to my question about using "reagent alcohol" instead of 100% ethanol. One further question - in plant histology its quite common to use alcohol in fixatives - i.e. FAA, FPA & Farmers. Does anybody have any experience in using 190 proof reagent alcohol in a fixative? I know it introduces small amounts of methanol and isopropanol into the "cocktail" but not sure if that really matters - Thanks for the advice/help! Mike Rio Grande Biological ------------------------------ Message: 7 Date: Fri, 11 Dec 2009 12:23:31 -0500 From: "Owen, Michael P" Subject: RE: [Histonet] alternates to absolute ethanol To: "Histonet" Message-ID: <6B1915839B67AD46B88E6BE8F27376BB19FBCC@FMD3VS031.fda.gov> Content-Type: text/plain; charset="us-ascii" Dear Michael, Have you tried absolute methanol? The cost of the reagent should be less expensive than absolute ethanol and is easier to obtain than absolute ethanol (i.e. less legal restrictions than absolute ethanol that can be diverted to non-laboratory use). When fixing bacterial smears for a variety of staining methods, I have discovered absolute methanol works well and is often superior to heat fixation. Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov ------------------------------ Message: 8 Date: Fri, 11 Dec 2009 09:36:01 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] maximum block cutting To: histonet-bounces@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, Cathy , ToniRathborne , jellin@yumaregional.org Message-ID: <80542.74357.qm@web65701.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 It is to be published in January 2010. If you "accept" 24 blocks/hour = 2.5 min/block Ren? J. --- On Fri, 12/11/09, jellin@yumaregional.org wrote: From: jellin@yumaregional.org Subject: Re: [Histonet] maximum block cutting To: "Rene J Buesa" , histonet-bounces@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, "Cathy" , "ToniRathborne" Date: Friday, December 11, 2009, 12:10 PM When was this data gathered... Some of this sounds excessive like 2.5 minutes a block??? Are these based on complete manual process or is automation also taken into consideration.? Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Date: Fri, 11 Dec 2009 08:47:45 To: ; ; Cathy; ToniRathborne Subject: RE: [Histonet] maximum block cutting Cutting : average of 2.5 min/block ( = 24/hour) Trimming: average of 25 secs./block ( =144/hour) Ren? J. --- On Fri, 12/11/09, Rathborne, Toni wrote: From: Rathborne, Toni Subject: RE: [Histonet] maximum block cutting To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, "Cathy" Date: Friday, December 11, 2009, 9:03 AM So what would the time be for trimming and cutting? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 4:29 PM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com; Cathy Subject: RE: [Histonet] maximum block cutting Just actual cutting. Trimming is considered another task. Ren? J. --- On Thu, 12/10/09, Cathy wrote: From: Cathy Subject: RE: [Histonet] maximum block cutting To: "'Rene J Buesa'" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com Date: Thursday, December 10, 2009, 12:22 PM Does this average include trimming into the block or just the actual cutting of the section? Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com Subject: Re: [Histonet] maximum block cutting The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/hour?with an average of 24 (sample size = 245 histolabs). Ren? J. --- On Wed, 12/9/09, histology@medsurgpath.com wrote: From: histology@medsurgpath.com Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law.? If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited.? If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ------------------------------ Message: 9 Date: Fri, 11 Dec 2009 09:40:15 -0800 (PST) From: Rene J Buesa Subject: RE: [Histonet] alternates to absolute ethanol To: Histonet , Michael POwen Message-ID: <436000.23257.qm@web65705.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The concern with methanol would be its toxicity. Methanol (TWA=200 ppm)?is 5 times more toxic than ethanol (TWA=1000 ppm) and twice more toxic than 2-propanol (TWA=400ppm). Ren? J. --- On Fri, 12/11/09, Owen, Michael P wrote: From: Owen, Michael P Subject: RE: [Histonet] alternates to absolute ethanol To: "Histonet" Date: Friday, December 11, 2009, 12:23 PM Dear Michael, Have you tried absolute methanol? The cost of the reagent should be less expensive than absolute ethanol and is easier to obtain than absolute ethanol (i.e. less legal restrictions than absolute ethanol that can be diverted to non-laboratory use). When fixing bacterial smears for a variety of staining methods, I have discovered absolute methanol works well and is often superior to heat fixation. Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE? Bothell, WA 98021-4421 Phone: 425-483-4865? ???E-Mail: michael.owen@fda.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 11 Dec 2009 17:10:58 +0000 From: jellin@yumaregional.org Subject: Re: [Histonet] maximum block cutting To: "Rene J Buesa" , histonet-bounces@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, "Cathy" , "ToniRathborne" Message-ID: <445413799-1260551459-cardhu_decombobulator_blackberry.rim.net-856900486-@bda047.bisx.prod.on.blackberry> Content-Type: text/plain; charset="Windows-1252" When was this data gathered... Some of this sounds excessive like 2.5 minutes a block?? Are these based on complete manual process or is automation also taken into consideration. Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Rene J Buesa Date: Fri, 11 Dec 2009 08:47:45 To: ; ; Cathy; ToniRathborne Subject: RE: [Histonet] maximum block cutting Cutting : average of 2.5 min/block ( = 24/hour) Trimming: average of 25 secs./block ( =144/hour) Ren? J. --- On Fri, 12/11/09, Rathborne, Toni wrote: From: Rathborne, Toni Subject: RE: [Histonet] maximum block cutting To: "Rene J Buesa" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com, "Cathy" Date: Friday, December 11, 2009, 9:03 AM So what would the time be for trimming and cutting? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 4:29 PM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com; Cathy Subject: RE: [Histonet] maximum block cutting Just actual cutting. Trimming is considered another task. Ren? J. --- On Thu, 12/10/09, Cathy wrote: From: Cathy Subject: RE: [Histonet] maximum block cutting To: "'Rene J Buesa'" , histonet@lists.utsouthwestern.edu, histology@medsurgpath.com Date: Thursday, December 10, 2009, 12:22 PM Does this average include trimming into the block or just the actual cutting of the section? Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM To: histonet@lists.utsouthwestern.edu; histology@medsurgpath.com Subject: Re: [Histonet] maximum block cutting The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/hour?with an average of 24 (sample size = 245 histolabs). Ren? J. --- On Wed, 12/9/09, histology@medsurgpath.com wrote: From: histology@medsurgpath.com Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ------------------------------ Message: 11 Date: Fri, 11 Dec 2009 10:53:08 -0700 From: Akemi Allison Subject: Re: [Histonet] Leica vs Ventana To: "Mahoney,Janice A" Cc: "histonet@lists.utsouthwestern.edu" , Sebree Linda A , "'Houston, Ronald'" , Kathleen Boozer Message-ID: <227D3462-0D2D-4971-A0A8-34E5585839B3@yahoo.com> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Hi Janice, I'm glad you brought up your point! It's amazing how people are. It just shows how narrow minded and unkind in their statements. Yes, Ventana is HUGE, but they have their faults as well! That being said, there is always room to build a better mouse trap and save money while doing it too! The new Bond is amazing and is capable of doing multiple IHC staining on (1) slide. It is also a open system. "Science never sleeps"! Thank you for speaking out, and hope you have a Wonderful Holiday Season! Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Dec 11, 2009, at 8:02 AM, Mahoney,Janice A wrote: > I think these obscure emails about vendors are very damaging, > regardless of who the vender is. If someone is experiencing a > particular problem with an instrument, please use this venue to get > advice on how others handled it. Be open about the problem so that > we all can learn from your experiences. To dog a company without > any real information is not useful to anyone. > Both companies have good instruments that may suit different types > of labs better than the other. > I'll get off my soap box now. > Jan Mahoney, Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald > Sent: Friday, December 11, 2009 8:55 AM > To: Edwards, Richard E.; Sebree Linda A; Kathleen Boozer; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > Probably depends on whether you're talking about a white elephant > or not > > Ronnie Houston > Anatomic Pathology Manager > Nationwide Children's Hospital > Columbus OH 43205 > (614) 722 5450 > > -----Original Message----- > From: Edwards, Richard E. [mailto:ree3@leicester.ac.uk] > Sent: Friday, December 11, 2009 9:52 AM > To: Houston, Ronald; Sebree Linda A; Kathleen Boozer; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > This sounds like the game children play, so who would win the fight > between an elephant and a hippopotamus, or the fight between a banana > and a pomegranate etcetc. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Houston, > Ronald > Sent: 11 December 2009 14:44 > To: Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > Not enough time in the day to answer that one > > Ronnie Houston > Anatomic Pathology Manager > Nationwide Children's Hospital > Columbus OH 43205 > (614) 722 5450 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree > Linda A > Sent: Friday, December 11, 2009 9:41 AM > To: Kathleen Boozer; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > Why would you switch from a reliable, walk-away, reproducible system? > > Just curious. > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Kathleen > Boozer > Sent: Friday, December 11, 2009 8:08 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica vs Ventana > > > Anyone out there found an advantage of going from Ventana to Leica for > IHC? I am being warned about extra costs and protocol issues with > the 3 > trays. > > In negotiations.... > > > Kathy Boozer, HT (ASCP), IHCQ > Adventist Medical Center > 10123 SE Market St. > Portland, OR 97216 > boozerka@ah.org > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel Health > Systems, Alegent Health is faithful to the healing ministry of > Jesus Christ, providing high quality care for the body, mind and > spirit of every person. > > The information contained in this communication, including > attachments, is confidential and private and intended only for the > use of the addressees. Unauthorized use, disclosure, distribution > or copying is strictly prohibited and may be unlawful. If you > received this communication in error, please inform us of the > erroneous delivery by return e-mail message from your computer. > Additionally, although all attachments have been scanned at the > source for viruses, the recipient should check any attachments for > the presence of viruses before opening. Alegent Health accepts no > liability for any damage caused by any virus transmitted by this e- > mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 73, Issue 18 **************************************** Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From Janice.Mahoney <@t> alegent.org Mon Dec 14 09:07:05 2009 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Mon Dec 14 09:07:19 2009 Subject: [Histonet] Leica vs Ventana In-Reply-To: <4B25BCEC.4AA8.00C0.0@ah.org> References: <7722595275A4DD4FA225B92CDBF174A1E8C9F7E017@EXC-MBX3.cfs.le.ac.uk> <979FF5962E234F45B06CF0DB7C1AABB21B645E7F@chi2k3ms01.columbuschildrens.net> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A54B6@EXCHMBC2.ad.ah.local> <227D3462-0D2D-4971-A0A8-34E5585839B3@yahoo.com> <4B25BCEC.4AA8.00C0.0@ah.org> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A54BD@EXCHMBC2.ad.ah.local> Kathleen, I did not take your question as offensive at all. Several of the replies were what I was referring to. Sorry for the confusion. Jan Mahoney Omaha -----Original Message----- From: Kathleen Boozer [mailto:BoozerKA@ah.org] Sent: Monday, December 14, 2009 6:20 AM To: Mahoney,Janice A; Akemi Allison Cc: Richard E. Edwards; histonet@lists.utsouthwestern.edu; Ronald' 'Houston; Sebree Linda A Subject: Re: [Histonet] Leica vs Ventana My sincere apologies to all that I have offended. I asked a simple question and got a very helpful reply from someone who asked me to call them, as they had compared for their facility and it answered my questions. Kathy >>> Akemi Allison 12/11/2009 09:53 >>> Hi Janice, I'm glad you brought up your point! It's amazing how people are. It just shows how narrow minded and unkind in their statements. Yes, Ventana is HUGE, but they have their faults as well! That being said, there is always room to build a better mouse trap and save money while doing it too! The new Bond is amazing and is capable of doing multiple IHC staining on (1) slide. It is also a open system. "Science never sleeps"! Thank you for speaking out, and hope you have a Wonderful Holiday Season! Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Dec 11, 2009, at 8:02 AM, Mahoney,Janice A wrote: > I think these obscure emails about vendors are very damaging, > regardless of who the vender is. If someone is experiencing a > particular problem with an instrument, please use this venue to get > advice on how others handled it. Be open about the problem so that > we all can learn from your experiences. To dog a company without > any real information is not useful to anyone. > Both companies have good instruments that may suit different types > of labs better than the other. > I'll get off my soap box now. > Jan Mahoney, Omaha > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald > Sent: Friday, December 11, 2009 8:55 AM > To: Edwards, Richard E.; Sebree Linda A; Kathleen Boozer; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > Probably depends on whether you're talking about a white elephant > or not > > Ronnie Houston > Anatomic Pathology Manager > Nationwide Children's Hospital > Columbus OH 43205 > (614) 722 5450 > > -----Original Message----- > From: Edwards, Richard E. [mailto:ree3@leicester.ac.uk] > Sent: Friday, December 11, 2009 9:52 AM > To: Houston, Ronald; Sebree Linda A; Kathleen Boozer; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > This sounds like the game children play, so who would win the fight > between an elephant and a hippopotamus, or the fight between a banana > and a pomegranate etcetc. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Houston, > Ronald > Sent: 11 December 2009 14:44 > To: Sebree Linda A; Kathleen Boozer; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > Not enough time in the day to answer that one > > Ronnie Houston > Anatomic Pathology Manager > Nationwide Children's Hospital > Columbus OH 43205 > (614) 722 5450 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree > Linda A > Sent: Friday, December 11, 2009 9:41 AM > To: Kathleen Boozer; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica vs Ventana > > Why would you switch from a reliable, walk-away, reproducible system? > > Just curious. > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Kathleen > Boozer > Sent: Friday, December 11, 2009 8:08 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica vs Ventana > > > Anyone out there found an advantage of going from Ventana to Leica for > IHC? I am being warned about extra costs and protocol issues with > the 3 > trays. > > In negotiations.... > > > Kathy Boozer, HT (ASCP), IHCQ > Adventist Medical Center > 10123 SE Market St. > Portland, OR 97216 > boozerka@ah.org > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel Health > Systems, Alegent Health is faithful to the healing ministry of > Jesus Christ, providing high quality care for the body, mind and > spirit of every person. > > The information contained in this communication, including > attachments, is confidential and private and intended only for the > use of the addressees. Unauthorized use, disclosure, distribution > or copying is strictly prohibited and may be unlawful. If you > received this communication in error, please inform us of the > erroneous delivery by return e-mail message from your computer. > Additionally, although all attachments have been scanned at the > source for viruses, the recipient should check any attachments for > the presence of viruses before opening. Alegent Health accepts no > liability for any damage caused by any virus transmitted by this e- > mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From east <@t> brinstrument.com Mon Dec 14 09:32:47 2009 From: east <@t> brinstrument.com (Kathryn Ebling) Date: Mon Dec 14 09:32:58 2009 Subject: [Histonet] Attention: Australia Customers Message-ID: Hello Histonetters, We would like to contact customers in Australia who have recently purchased B/R Instrument 9700 Mini-ProCyclerT Recycling Systems. We have a customer satisfaction survey we would like to pass along. Hope to hear from you! Please contact me at: east@brinstrument.com Thank you, Kathryn Ebling Clinical Sales Manager B/R Instrument Corporation +1 410-820-1494 east@brinstrument.com From Margaret.Perry <@t> sdstate.edu Mon Dec 14 10:17:34 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Mon Dec 14 10:17:41 2009 Subject: [Histonet] alcian blue stain Message-ID: We are really getting new research procedures lately. As with almost all published papers the methods are a quick overview without details. We have been asked to do a combination stain of H&E with alcian blue. Would you recommend doing the alcian blue 8GX pH 2.5 first, doing it after the hematoxylin or after the eosin? I'm concerned about the eosin bleeding out and the possible change in pH affecting the stains. Margaret Perry From bhewlett <@t> cogeco.ca Mon Dec 14 10:30:13 2009 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon Dec 14 10:30:21 2009 Subject: [Histonet] alcian blue stain References: Message-ID: <28669B8E3DE040A595A60852E62537FC@mainbox> Perform the Alcian blue first, then follow with the H&E. Done it hundreds of times, no problem. Bryan ----- Original Message ----- From: "Perry, Margaret" To: Sent: Monday, December 14, 2009 11:17 AM Subject: [Histonet] alcian blue stain We are really getting new research procedures lately. As with almost all published papers the methods are a quick overview without details. We have been asked to do a combination stain of H&E with alcian blue. Would you recommend doing the alcian blue 8GX pH 2.5 first, doing it after the hematoxylin or after the eosin? I'm concerned about the eosin bleeding out and the possible change in pH affecting the stains. Margaret Perry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail <@t> bellsouth.net Mon Dec 14 10:38:52 2009 From: renafail <@t> bellsouth.net (Rena Fail) Date: Mon Dec 14 10:38:57 2009 Subject: [Histonet] alcian blue stain In-Reply-To: References: Message-ID: <672860.43827.qm@web180309.mail.gq1.yahoo.com> Alcian blue first, the resulting stain is insoluble in water alcohol Rena Fail ----- Original Message ---- From: "Perry, Margaret" To: "histonet@lists.utsouthwestern.edu" Sent: Mon, December 14, 2009 11:17:34 AM Subject: [Histonet] alcian blue stain We are really getting new research procedures lately.? As with almost all published papers the methods are a quick overview without details.? We have been asked to do a combination stain of H&E with alcian blue.? Would you recommend doing the alcian blue 8GX pH 2.5 first, doing it after the hematoxylin or after the eosin?? I'm concerned about the eosin bleeding out and the possible change in pH affecting the stains. Margaret Perry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nancy_schmitt <@t> pa-ucl.com Mon Dec 14 10:43:10 2009 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Mon Dec 14 10:43:15 2009 Subject: [Histonet] (no subject) Message-ID: <737BD0BF52F0744B96B74B61756AC06441638BB137@hestia.ad.pa-ucl.com> Good Morning We are running paps on Tripath system. We are having trouble with fungal contaminant. It has been an ongoing issue - we now filter all stains before placing on system (along with bleaching the bottles and caps) and daily rinse the lines with bleach and then type 1 water. It is now a random sighting, but nevertheless we are still randomly seeing this. Does anyone have any thoughts? Thanks for your time Nancy Schmitt HT(ASCP), MLT(CSMLS) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From tjay30 <@t> yahoo.com Mon Dec 14 11:12:13 2009 From: tjay30 <@t> yahoo.com (Timothy Jay) Date: Mon Dec 14 11:12:15 2009 Subject: [Histonet] job opportunity in N. California Message-ID: <272787.32650.qm@web34307.mail.mud.yahoo.com> Part Time Histology Tech Needed: Exciting opportunity for a licensed Histology Technician to work in a new lab located in the Yuba City/Marysville, California area. Please send resume to Timothy Garcia-Jay at tjay30@yahoo.com or call 775-830-1591. From rmanalac <@t> cahfs.ucdavis.edu Mon Dec 14 11:24:17 2009 From: rmanalac <@t> cahfs.ucdavis.edu (Manalac, Rosa) Date: Mon Dec 14 11:24:22 2009 Subject: [Histonet] Lab chair Message-ID: Can anyone suggest a comfortable and ergonomic chair to use while working with the microtome for many hours each day? I would appreciate information on the brand, model, or type and where to order it. Even a website would help. Thank you. From brian <@t> prometheushealthcare.com Mon Dec 14 11:26:58 2009 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Mon Dec 14 11:27:03 2009 Subject: [Histonet] Histotechnologist Openings in New York Message-ID: <001301ca7ce2$a3d99800$eb8cc800$@com> Prometheus Healthcare, New York's # 1 Recruiter for Laboratory professionals, is currently seeking technicians and technologists for numerous histology opportunities throughout New York City and the surrounding areas. Shifts vary from day, evening to night and opportunities are available within grossing, embedding, cutting, staining, and IHC. Supervisor positions are also available. The facilities we work with include some of the most well known hospitals, reference labs and biotech companies. Depending on position and location, sign on bonuses and generous shift differentials are offered as well! Please contact us today at 301-693-9057 to discuss our current openings. Please visit our website at www.prometheushealthcare.com ****Also be sure to join us on Twitter to stay up to date on our newest lab positions**** http://twitter.com/PrometheusBlog Requirements Must have current NY technician or technologist license ASCP preferred At least 1 year histology experience Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From Janet.Bonner <@t> FLHOSP.ORG Mon Dec 14 12:23:25 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon Dec 14 12:27:20 2009 Subject: [Histonet] Lab chair References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2BFF@fhosxchmb006.ADVENTISTCORP.NET> We had a great company that used distributors and right after we bought the workchairs, they went somewhere else! Google Ergonomic workchair and you should get a good selection. Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Manalac, Rosa Sent: Mon 12/14/2009 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lab chair Can anyone suggest a comfortable and ergonomic chair to use while working with the microtome for many hours each day? I would appreciate information on the brand, model, or type and where to order it. Even a website would help. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From akemiat3377 <@t> yahoo.com Mon Dec 14 12:36:51 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Mon Dec 14 12:37:56 2009 Subject: [Histonet] Lab chair In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47807F2BFF@fhosxchmb006.ADVENTISTCORP.NET> References: <5F31F38C96781A4FBE3196EBC22D47807F2BFF@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: Whatever chair you choose, make sure you meet you lab safety requirements. In one of the lab's I supervised, we were dinged for not having chairs with (5) verses (4) legs when we went through an inspection. We had to replace all our lab chairs to meet the required standards. I am not sure if this is a set standard, or it varies from State to State. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Dec 14, 2009, at 11:23 AM, Bonner, Janet wrote: > We had a great company that used distributors and right after we > bought the workchairs, they went somewhere else! Google Ergonomic > workchair and you should get a good selection. > Janet > > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Manalac, Rosa > Sent: Mon 12/14/2009 12:24 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Lab chair > > > > Can anyone suggest a comfortable and ergonomic chair to use while > working with the microtome for many hours each day? I would > appreciate > information on the brand, model, or type and where to order it. > Even a > website would help. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ======================================================= > The information contained in this message may be privileged and/or > confidential > and protected from disclosure. If the reader of this message is > not the intended > recipient or an employee or agent responsible for delivering this > message to the > intended recipient, you are hereby notified that any dissemination, > distribution > or copying of this communication is strictly prohibited. If you > have received this > communication in error, please notify the sender immediately by > replying to this > message and deleting the material from any computer. > ======================================================= > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Mon Dec 14 14:36:22 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon Dec 14 14:37:53 2009 Subject: [Histonet] Lab chair References: <5F31F38C96781A4FBE3196EBC22D47807F2BFF@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2C00@fhosxchmb006.ADVENTISTCORP.NET> The chairs have to have vinyl seats and back supports, not cloth, so they can be easily washed. Janet ________________________________ From: Akemi Allison [mailto:akemiat3377@yahoo.com] Sent: Mon 12/14/2009 1:36 PM To: Bonner, Janet Cc: Manalac, Rosa; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Lab chair Whatever chair you choose, make sure you meet you lab safety requirements. In one of the lab's I supervised, we were dinged for not having chairs with (5) verses (4) legs when we went through an inspection. We had to replace all our lab chairs to meet the required standards. I am not sure if this is a set standard, or it varies from State to State. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Dec 14, 2009, at 11:23 AM, Bonner, Janet wrote: We had a great company that used distributors and right after we bought the workchairs, they went somewhere else! Google Ergonomic workchair and you should get a good selection. Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Manalac, Rosa Sent: Mon 12/14/2009 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lab chair Can anyone suggest a comfortable and ergonomic chair to use while working with the microtome for many hours each day? I would appreciate information on the brand, model, or type and where to order it. Even a website would help. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Vickroy.Jim <@t> mhsil.com Mon Dec 14 14:52:11 2009 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Dec 14 14:52:13 2009 Subject: [Histonet] microwave tissue processors Message-ID: <24A4826E8EF0964D86BC5317306F58A5425465B314@mmc-mail.ad.mhsil.com> I have asked the question before but need any updated information you can share. We are purchasing a microwave tissue processor and are researching what will meet our needs the best. I would be interested in colleagues sharing what problems they have had with the instruments they purchased, such as the Milestone Pathos and the Sakura Express. Thank you. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From POWELL_SA <@t> mercer.edu Mon Dec 14 15:23:57 2009 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Dec 14 15:24:03 2009 Subject: [Histonet] GSH meeting Message-ID: <9BF995BC0E47744E9673A41486E24EE222A59B7A87@MERCERMAIL.MercerU.local> The Georgia Society for Histotechnology Annual meeting will be held March 26-28, 2010 at Stone Mountain, the complete hotel information is listed below. We have a few more slots open for our meeting. The abstract form to fill out may be downloaded from the NSH website, www.nsh.org or you may respond to this message and I will send one to you. Please mail completed forms to me as soon as possible or email them. My physical address is: Shirley Powell, HT(ASCP)HTL, QIHC GSH Secretary Mercer University School of Medicine 1550 College Street Macon, GA 31207 Note:********If a session has been approved by NSH in the past, a complete abstract form is not necessary as long as the complete abstract is provided to GSH for the program printing, along with complete presenter information. Please contact me for more information if needed. Evergreen Marriott(r) Conference Resort 4021 Lakeview Drive Stone Mountain, Georgia 30083 USA Toll-free: 1-888-670-2250 http://www.marriott.com/hotels/hotel-photos/ATLEG/?mktcmp=w_regionsite_atleg_x Room rates are $99 CALL FOR YOUR RESERVATIONS TODAY. BRING THE FAMILY TO ATLANTA AND ENJOY STONE MOUNTAIN, THE PARKS AND OTHER AREA ATTRACTIONS. Shirley A. Powell, HT(ASCP)HTL, QIHC Technical Director Histology Curricular Support Laboratory Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 Lab 478-301-5489 Fax From mwich <@t> 7thwavelabs.com Mon Dec 14 15:30:52 2009 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Mon Dec 14 15:31:54 2009 Subject: [Histonet] IHC macrophage marker for demineralized bone Message-ID: <62A8156F8071C8439080D626DF8C33A65D8ED4@wave-mail.7thwave.local> Can anyone recommend an antibody against rat macrophages which will work in FFPE de-mineralized bone sections? F4/80 possibly? Is Immuno-cal the best decalcifying solution for IHC procedures? I would greatly appreciate any suggestions. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From liz <@t> premierlab.com Mon Dec 14 15:46:42 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Dec 14 15:47:17 2009 Subject: [Histonet] IHC macrophage marker for demineralized bone References: <62A8156F8071C8439080D626DF8C33A65D8ED4@wave-mail.7thwave.local> Message-ID: F4/80 does work with FFPE formic acid decalcifed samples. LIz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Michele Wich Sent: Mon 12/14/2009 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC macrophage marker for demineralized bone Can anyone recommend an antibody against rat macrophages which will work in FFPE de-mineralized bone sections? F4/80 possibly? Is Immuno-cal the best decalcifying solution for IHC procedures? I would greatly appreciate any suggestions. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Dec 14 15:48:26 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 14 15:48:29 2009 Subject: [Histonet] IHC macrophage marker for demineralized bone In-Reply-To: <62A8156F8071C8439080D626DF8C33A65D8ED4@wave-mail.7thwave.local> Message-ID: <876074.64462.qm@web65713.mail.ac4.yahoo.com> Since Immuno-cal is a commercial decalcifyer it probably was developed after testing and is probably?good, but I always used EDTA as a chelating agent for IHC. Ren? J. ? ? --- On Mon, 12/14/09, Michele Wich wrote: From: Michele Wich Subject: [Histonet] IHC macrophage marker for demineralized bone To: histonet@lists.utsouthwestern.edu Date: Monday, December 14, 2009, 4:30 PM Can anyone recommend an antibody against rat macrophages which will work in FFPE de-mineralized bone sections? F4/80 possibly? Is Immuno-cal the best decalcifying solution for IHC procedures? I would greatly appreciate any suggestions. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure.? If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited.? Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Mon Dec 14 17:36:25 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Dec 14 17:36:38 2009 Subject: [Histonet] IHC macrophage marker for demineralized bone In-Reply-To: <62A8156F8071C8439080D626DF8C33A65D8ED4@wave-mail.7thwave.local> References: <62A8156F8071C8439080D626DF8C33A65D8ED4@wave-mail.7thwave.local> Message-ID: <000301ca7d16$41ca7420$c55f5c60$@callis@bresnan.net> You will probably have to use EDTA, there is a whole subset of rat macrophages, ED1, ED2, ED3 (Serotec has these) and you should know which macrophage subset you need to see. Sorry, F4/80 is for mouse macrophages not rat macrophages. Rat macrophages may be very sensitive to any type of acid decalcification although people report successful staining with formalin fixed paraffin embedded tissues which means you could use EDTA instead of an acid. If you go to SEROTEC, and look up the antibody data sheets, you will see what application is going to work best for rat macrophages e.g. FFPE, frozen sections only, or some other fixation such as PLP (paraformaldehyde/lysine/periodate). Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Monday, December 14, 2009 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC macrophage marker for demineralized bone Can anyone recommend an antibody against rat macrophages which will work in FFPE de-mineralized bone sections? F4/80 possibly? Is Immuno-cal the best decalcifying solution for IHC procedures? I would greatly appreciate any suggestions. __________ Information from ESET Smart Security, version of virus signature database 4684 (20091213) __________ The message was checked by ESET Smart Security. http://www.eset.com From liz <@t> premierlab.com Mon Dec 14 18:21:30 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Dec 14 18:23:57 2009 Subject: [Histonet] IHC macrophage marker for demineralized bone References: <62A8156F8071C8439080D626DF8C33A65D8ED4@wave-mail.7thwave.local> <000301ca7d16$41ca7420$c55f5c60$@callis@bresnan.net> Message-ID: Gayle corrected me that since you are staining rat tissue you will need to stain for ED-1. We have had sucess with staining rat ED-1 and ED-2 on FFPE formic acid decaled samples using the serotec antibodies. Liz ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of gayle callis Sent: Mon 12/14/2009 4:36 PM To: 'Michele Wich'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC macrophage marker for demineralized bone You will probably have to use EDTA, there is a whole subset of rat macrophages, ED1, ED2, ED3 (Serotec has these) and you should know which macrophage subset you need to see. Sorry, F4/80 is for mouse macrophages not rat macrophages. Rat macrophages may be very sensitive to any type of acid decalcification although people report successful staining with formalin fixed paraffin embedded tissues which means you could use EDTA instead of an acid. If you go to SEROTEC, and look up the antibody data sheets, you will see what application is going to work best for rat macrophages e.g. FFPE, frozen sections only, or some other fixation such as PLP (paraformaldehyde/lysine/periodate). Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Monday, December 14, 2009 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC macrophage marker for demineralized bone Can anyone recommend an antibody against rat macrophages which will work in FFPE de-mineralized bone sections? F4/80 possibly? Is Immuno-cal the best decalcifying solution for IHC procedures? I would greatly appreciate any suggestions. __________ Information from ESET Smart Security, version of virus signature database 4684 (20091213) __________ The message was checked by ESET Smart Security. http://www.eset.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Mon Dec 14 18:43:30 2009 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Dec 14 18:43:35 2009 Subject: [Histonet] alcian blue stain Message-ID: <582736990912141643s3a25ea9q3691328bf68999df@mail.gmail.com> Hi, I agree with the other suggestions to do the Alcian Blue first. I would, however take a page from the Movat Pentachrome stain and add an alkaline alcohol step (10% ammonium hydroxide in 95% ethanol) to convert the Alcian Blue to an insoluble stain (monastral fast blue). It may not bleach out in subsequent steps, but this step will make sure of it. Amos Message: 11 Date: Mon, 14 Dec 2009 10:17:34 -0600 From: "Perry, Margaret" Subject: [Histonet] alcian blue stain To: "histonet@lists.utsouthwestern.edu" Message-ID: < FCA5EF47F9BC694CBB4C58FEA042196348876BA179@SDSU-MBX.jacks.local> Content-Type: text/plain; charset="us-ascii" We are really getting new research procedures lately. As with almost all published papers the methods are a quick overview without details. We have been asked to do a combination stain of H&E with alcian blue. Would you recommend doing the alcian blue 8GX pH 2.5 first, doing it after the hematoxylin or after the eosin? I'm concerned about the eosin bleeding out and the possible change in pH affecting the stains. Margaret Perry From dellav <@t> musc.edu Tue Dec 15 08:28:38 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Dec 15 08:28:52 2009 Subject: [Histonet] RE: microwave tissue processors In-Reply-To: <24A4826E8EF0964D86BC5317306F58A5425465B314@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A5425465B314@mmc-mail.ad.mhsil.com> Message-ID: I would also like to receive this information should individuals choose to respond directly. thanks Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, December 14, 2009 3:52 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave tissue processors I have asked the question before but need any updated information you can share. We are purchasing a microwave tissue processor and are researching what will meet our needs the best. I would be interested in colleagues sharing what problems they have had with the instruments they purchased, such as the Milestone Pathos and the Sakura Express. Thank you. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Tue Dec 15 08:52:29 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Dec 15 08:52:35 2009 Subject: [Histonet] Blade Dispenser Thingy Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46CE6@nmdamailsvr.nmda.ad.nmsu.edu> I have Feather dispensers with blades that I have to pry out, which is probably not a good thing. I've tried freezing the dispenser, putting it in an oven for a while and whacking the darned thing on the floor (not necessarily all at the same time..). Have we had this discussion before and have we solved this problem? Help? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From sfp1968 <@t> gmail.com Tue Dec 15 09:11:22 2009 From: sfp1968 <@t> gmail.com (Terry OBrien) Date: Tue Dec 15 09:11:28 2009 Subject: [Histonet] Fwd: A New Tissue Microarryer Instrument - Pathology In-Reply-To: References: Message-ID: Please ask them to remove this advertisment. Histonet is not for this purpose. ---------- Forwarded message ---------- From: Thom Jensen Date: Mon, Dec 14, 2009 at 9:34 PM Subject: A New Tissue Microarryer Instrument - Pathology To: sfp1968@gmail.com Dear Histologists, Pathologists and Researchers; I am writing to let you know of a new Tissue Microarray (TMA) instrument on the market. The Arraymold. This simple and easy to use TMA instrument can be used for Paraffin and Frozen tissues. It is small enough to store in a drawer instead of taking up valuable counter top space. The Arraymold comes with a simple to follow DVD and 10 extra needles. Please visit our website to *watch a video on how to use the Arraymold*and learn more about this wonderful TMA instrument. www.arraymold.com Kind regards, Thom Jensen President, Arraymold, LC *Beneficial ways of using the Arraymold.* 1. Allows a pathologist or researcher to view many different samples at once instead of hundreds of slides. 2. Helpful in finding IHC markers for controls or quality assurance using hundreds of tissues samples on a few slides instead of hundreds of slides. 3. Consistency in staining markers because everything is on one slide. 4. Validating new instruments or new antibodies to be used in the laboratory while keeping antibody cost down significantly. 5. Using the Arraymold only once can pay for its self: a. Instead of 60 to 150 slides with expensive antibodies now you use 1 slide. b. Also add up the amount of hours it takes to cut and stain hundreds of slides individually. "Some day the Tissue Microarray instrument will be as common in the laboratory as the microtome..." From gvdobbin <@t> ihis.org Tue Dec 15 09:19:17 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Tue Dec 15 09:19:54 2009 Subject: [Histonet] Blade Dispenser Thingy Message-ID: Hi Sally, Fisher is aware of the problem (or should be). I contacted Fisher Canada about it probably a year ago or so and had them replace 4 boxes for me. They made it seem as though this was a brand new phenomenon that they had not heard of before but I've known it to be a sporadic problem for years and I figured I couldn't have been the only one to have experienced this problem! They logged my complaint and forwarded the information on to the manufacturer (a Japonese company I believe). But as yet, I have't seen any design improvements to the dispensers. Maybe a Fisher person on this list would like to comment on whether the manufacturer has made any progress toward improving the dispensers. Cheers. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Breeden, Sara" 12/15/2009 10:52 AM >>> I have Feather dispensers with blades that I have to pry out, which is probably not a good thing. I've tried freezing the dispenser, putting it in an oven for a while and whacking the darned thing on the floor (not necessarily all at the same time..). Have we had this discussion before and have we solved this problem? Help? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. From valerio2 <@t> buffalo.edu Tue Dec 15 09:20:44 2009 From: valerio2 <@t> buffalo.edu (Mike Valerio) Date: Tue Dec 15 09:20:48 2009 Subject: [Histonet] Help with IHC Message-ID: <2684.1260890444@buffalo.edu> Hello, I would like to stain FFPE mouse brain tissue with MCP-1 antibody from serotec. I was wondering if anyone who uses this marker could share their protocol and indicate what is a good positive control. Thanks in advance. Michael Valerio University at Buffalo valerio2@buffalo.edu 716-829-5650 From NKlemme <@t> sakuraus.com Tue Dec 15 09:23:07 2009 From: NKlemme <@t> sakuraus.com (Nancy Klemme) Date: Tue Dec 15 09:24:24 2009 Subject: [Histonet] RE: Blade Dispenser Thingy In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46CE6@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46CE6@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <782E3A02C2EB2347BEA6DEA69DC7AB8669D9A01798@sfamail.SAKURAUS.LOCAL> Dear Sally, If these are Accu-Edge low or high-profile blades, I would strongly recommend that you call whomever these were purchased from with the following information: 1 - Product identifier / description and catalog or part number 2 - Product LOT number 3 - Date of purchase (if unavailable, approximate) 4 - Purchase order info. 5 - COMPLAINT / description of problem There is a certain risk level in working with knives and blades anyway, and difficulty in dispensing is an increased risk that would not be acceptable. Request replacement and a Return Authorization Number to return the same number you are requesting to have replaced. Kind regards, Nancy Klemme, Edu.Svcs.Dir - Sakura Finetek USA, Inc -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, December 15, 2009 6:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Dispenser Thingy I have Feather dispensers with blades that I have to pry out, which is probably not a good thing. I've tried freezing the dispenser, putting it in an oven for a while and whacking the darned thing on the floor (not necessarily all at the same time..). Have we had this discussion before and have we solved this problem? Help? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KLAPANO1 <@t> hfhs.org Tue Dec 15 09:27:10 2009 From: KLAPANO1 <@t> hfhs.org (Lapanowski, Karen) Date: Tue Dec 15 09:27:20 2009 Subject: [Histonet] OX6/CD74 Message-ID: Hello, I want to stain for Ox6/CD74 in rat brain paraffin sections. Any suggestions for a vendor and protocol? Thanks! ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From SDrew <@t> uwhealth.org Tue Dec 15 09:35:12 2009 From: SDrew <@t> uwhealth.org (Drew Sally A) Date: Tue Dec 15 09:35:24 2009 Subject: [Histonet] IHC for renin? Message-ID: <738A7878143FF74BB77436E255743C1A1F7D35@UWHC-MAIL03.uwhis.hosp.wisc.edu> We have a request for renin on a patient case. Is there a reference lab that does this? I've checked our usual send out places with no luck. Thank you for any info! Sally Ann Drew, MT (ASCP) sdrew@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 From NMargaryan <@t> childrensmemorial.org Tue Dec 15 12:00:54 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Tue Dec 15 12:01:29 2009 Subject: [Histonet] FW: Notch-Nodal Paper Message-ID: Hi histonetters, I am looking for some dye to be used in vivo like cell tracker or similar might work for identifying live cells. Any suggestions are appreciated, Naira From Janet.Bonner <@t> FLHOSP.ORG Tue Dec 15 12:51:23 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Dec 15 12:55:12 2009 Subject: [Histonet] Blade Dispenser Thingy References: <4D14F0FC9316DD41972D5F03C070908B02E46CE6@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2C06@fhosxchmb006.ADVENTISTCORP.NET> I keep the dispenser in a small plastic bag that seals along with the desiccant packages they put in the original box with the blades, (and any other desiccant packages I can find - from coverslips, inks etc.) As long as you keep the blades sealed with the desiccant, you shouldn't have anymore problems. This works really well in the cryostat chamber, too! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Tue 12/15/2009 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Dispenser Thingy I have Feather dispensers with blades that I have to pry out, which is probably not a good thing. I've tried freezing the dispenser, putting it in an oven for a while and whacking the darned thing on the floor (not necessarily all at the same time..). Have we had this discussion before and have we solved this problem? Help? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Maria.Katleba <@t> stjoe.org Tue Dec 15 13:39:48 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Dec 15 13:40:12 2009 Subject: [Histonet] RE: Blade Dispenser Thingy In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47807F2C06@fhosxchmb006.ADVENTISTCORP.NET> References: <4D14F0FC9316DD41972D5F03C070908B02E46CE6@nmdamailsvr.nmda.ad.nmsu.edu> <5F31F38C96781A4FBE3196EBC22D47807F2C06@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: I use one drop of microtome oil and they slip right out. I mean don't go crazy with the oil either! Maria Katleba HT(ASCP) MS Napa CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Tuesday, December 15, 2009 10:51 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blade Dispenser Thingy I keep the dispenser in a small plastic bag that seals along with the desiccant packages they put in the original box with the blades, (and any other desiccant packages I can find - from coverslips, inks etc.) As long as you keep the blades sealed with the desiccant, you shouldn't have anymore problems. This works really well in the cryostat chamber, too! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Tue 12/15/2009 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Dispenser Thingy I have Feather dispensers with blades that I have to pry out, which is probably not a good thing. I've tried freezing the dispenser, putting it in an oven for a while and whacking the darned thing on the floor (not necessarily all at the same time..). Have we had this discussion before and have we solved this problem? Help? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From Maria.Katleba <@t> stjoe.org Tue Dec 15 14:05:00 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Dec 15 14:05:32 2009 Subject: [Histonet] Histology Jobs Outside the USA Message-ID: Anyone know where you can find Histology positions OUTSIDE the USA? I have had several colleagues ask... and I am actually interested in this information as well..... Any thoughts? Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From Pat.Bell <@t> ucdenver.edu Tue Dec 15 16:00:18 2009 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Tue Dec 15 16:00:22 2009 Subject: [Histonet] COX2 in mice Message-ID: <64DB27005E2FD3439E88502D7A5C91218E0894A64B@CORTEZ.ucdenver.pvt> Does anyone know of a good antibody for COX2 that works in the mouse? Thank you, Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu From rachelr <@t> nei.nih.gov Tue Dec 15 16:23:00 2009 From: rachelr <@t> nei.nih.gov (Rachel, Rivka (NIH/NEI) [E]) Date: Tue Dec 15 16:19:26 2009 Subject: [Histonet] Anti-Flag antibody Message-ID: Does anyone know of a good rabbit anti-Flag antibody that can be used on transfected mouse cells? -- Rivka A. Rachel National Eye Institute From liz <@t> premierlab.com Tue Dec 15 17:21:12 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Dec 15 17:21:18 2009 Subject: [Histonet] Leica Bond Message-ID: Hey everyone I have a question regarding the Leica Bond IHC stainer. We are currently evaluating it. Is it true that you can not use a protein block on the instrument and if you do you have to rinse afterwards. I know that a lot of labs do not use protein blocks anymore but we routinely use them here, we use a serum free protein block on all of our IHC slides. If there is anyone out there that is willing to give me some advice on this? Also if anyone wants to comment on the use or lack of use of protein block that would be greatly appreciated also. I'm beginning to think I may be old fashioned but I'm a bit concerned about not using protein block, OMG I hope I'm not turning into one of those techs who has been in the field so long that they have become resistant to change, yikes! Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 From Beatrice.Debrosse-Serra <@t> pfizer.com Tue Dec 15 17:29:58 2009 From: Beatrice.Debrosse-Serra <@t> pfizer.com (Debrosse-Serra, Beatrice) Date: Tue Dec 15 17:30:05 2009 Subject: [Histonet] Leica Bond In-Reply-To: References: Message-ID: Hi Liz, We have Bonds and use protein blocks all the time. It isn't part of the kit, but you can use it in one of the open containers. Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, December 15, 2009 3:21 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Leica Bond Hey everyone I have a question regarding the Leica Bond IHC stainer. We are currently evaluating it. Is it true that you can not use a protein block on the instrument and if you do you have to rinse afterwards. I know that a lot of labs do not use protein blocks anymore but we routinely use them here, we use a serum free protein block on all of our IHC slides. If there is anyone out there that is willing to give me some advice on this? Also if anyone wants to comment on the use or lack of use of protein block that would be greatly appreciated also. I'm beginning to think I may be old fashioned but I'm a bit concerned about not using protein block, OMG I hope I'm not turning into one of those techs who has been in the field so long that they have become resistant to change, yikes! Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Tue Dec 15 23:39:53 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Dec 15 23:39:58 2009 Subject: [Histonet] FW: Notch-Nodal Paper In-Reply-To: References: Message-ID: Dear Naira Margaryan, Please clarify "in vivo", which means "in alive ...". What is your "..."? Do you want to stain living cells in thin-layer tissue cultures, suspended cells (blood, haemolymph etc), or cells in whole organisms that are small enough to live under a coverslip (such as rotifers, little nematodes and protozoans)? For larger animals, in vivo microscopy is possible only at the surface (skin and other accessible surfaces) or where there are transparent media (the eye). Beware of adverts for fluorescent stains with catchy names! If you hope to be a career scientist you will run into the peer review process, which is severe and does not like results based on unexplained trade secrets. If you explain your question on histonet, you will get plenty of good advice, supplemented with peer-reviewed references that you can check. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Margaryan, Naira" Date: Tuesday, December 15, 2009 13:02 Subject: [Histonet] FW: Notch-Nodal Paper To: "histonet@lists.utsouthwestern.edu" > Hi histonetters, > > I am looking for some dye to be used in vivo like cell tracker > or similar might work for identifying live cells. > > Any suggestions are appreciated, > Naira > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcampbell <@t> vdxpathology.com Wed Dec 16 10:20:29 2009 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Wed Dec 16 10:20:34 2009 Subject: [Histonet] any info on Glutathione S-Transferase Pi (GST Pi)? Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF82FA3D1@VDXSERVER01.vdxpathology.local> Hi All, Has anyone ever used this antibody in FFPE mouse or rat tissue? How easy of an antibody is it to work with? Other comments? We may be doing this for an upcoming project and I just wanted to know what I could be getting myself into. Thanks! Jen Campbell From ryaskovich <@t> dir.nidcr.nih.gov Wed Dec 16 11:03:29 2009 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Wed Dec 16 11:03:37 2009 Subject: [Histonet] Equipment Repair Message-ID: Does anyone know of a company that repairs equipment in the Maryland area? I'm in Bethesda and my old Tissue-Tek embedding center won't hold the temperature. Thanks for any help, Ruth Yaskovich National Institutes of Health Bethesda, Maryland From cruffin <@t> valleycare.com Wed Dec 16 12:12:43 2009 From: cruffin <@t> valleycare.com (Carole Ruffin) Date: Wed Dec 16 12:12:59 2009 Subject: [Histonet] New processor help Message-ID: <4B28B29B020000F200003CCB@mail.valleycare.com> Is there a procedure or some standard method validation process out there for starting up a new tissue processor? Maybe one that would be approved by regulatory agency? I am not sure whether this would just be left up to the pathologists to decide what they would prefer. Thank you, Carole Ruffin, HT(ASCP) ValleyCare Hospital Pleasanton, CA This message and any included attachments are from ValleyCare Health System and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. From DKBoyd <@t> chs.net Wed Dec 16 12:24:10 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Dec 16 12:23:15 2009 Subject: [Histonet] FNA stain Message-ID: This question is for those of you who perform fine needle aspirations. What stain are you using for your immediate evaluation? Or do you give an immediate evaluation/adequacy? Thanks. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From jcampbell <@t> vdxpathology.com Wed Dec 16 12:34:40 2009 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Wed Dec 16 12:34:45 2009 Subject: [Histonet] leaving IHC slides in wash buffer Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF82FA3F0@VDXSERVER01.vdxpathology.local> Hi Everyone, I have yet another IHC-related question. So after antigen retrieval, and then allowing my slides too cool, I place them in wash buffer before loading them on to the autostainer. Would leaving the slides in the wash buffer in the fridge for an extended period of time (say, 1 week) before continuing with immunostaining affect staining quality in any way? Thank you, Jen Campbell From Theresa.Oeler <@t> nsabp.org Wed Dec 16 12:34:56 2009 From: Theresa.Oeler <@t> nsabp.org (Oeler, Theresa) Date: Wed Dec 16 12:35:04 2009 Subject: [Histonet] Repairs to equipment. References: <20091216181931.2D4ECF4A3C@Draconis.nsabp.org> Message-ID: <3143A79C6CDE654183334C7FD94C1B7D02E3917F@mercury.NSABP.org> Hi, We use Tech One Biomedical Services, Inc. as a third party PM and repair service. They are very nice to work with and not excessive with their fees. We have set up PM's with them for a lot of our older equipment. Toll free number is 866-497-3033 or www.techoneweb.com . Try them for your repairs, we have no problems with them for the four years we have been dealing with them. No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. Theresa A Oeler, BS Senior Research Histologist NSABP Pathology Laboratory Federal North Building 1307 Federal Street, Suite 303 Pittsburgh, PA 15212 412/359-8931 Of. 412/359-3239 Fx. theresa.oeler@nsabp.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, December 16, 2009 1:20 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 73, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. FW: Notch-Nodal Paper (Margaryan, Naira) 2. RE: Blade Dispenser Thingy (Bonner, Janet) 3. RE: Blade Dispenser Thingy (Maria Katleba) 4. Histology Jobs Outside the USA (Maria Katleba) 5. COX2 in mice (Bell, Pat) 6. Anti-Flag antibody (Rachel, Rivka (NIH/NEI) [E]) 7. Leica Bond (Liz Chlipala) 8. RE: Leica Bond (Debrosse-Serra, Beatrice) 9. Re: FW: Notch-Nodal Paper (John Kiernan) 10. any info on Glutathione S-Transferase Pi (GST Pi)? (Jennifer Campbell) 11. Equipment Repair (Yaskovich, Ruth A (NIH/NIDCR) [E]) ---------------------------------------------------------------------- Message: 1 Date: Tue, 15 Dec 2009 12:00:54 -0600 From: "Margaryan, Naira" Subject: [Histonet] FW: Notch-Nodal Paper To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi histonetters, I am looking for some dye to be used in vivo like cell tracker or similar might work for identifying live cells. Any suggestions are appreciated, Naira ------------------------------ Message: 2 Date: Tue, 15 Dec 2009 13:51:23 -0500 From: "Bonner, Janet" Subject: RE: [Histonet] Blade Dispenser Thingy To: "Breeden, Sara" , histonet@lists.utsouthwestern.edu Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2C06@fhosxchmb006.ADVENTISTCORP.NET> Content-Type: text/plain; charset=iso-8859-1 I keep the dispenser in a small plastic bag that seals along with the desiccant packages they put in the original box with the blades, (and any other desiccant packages I can find - from coverslips, inks etc.) As long as you keep the blades sealed with the desiccant, you shouldn't have anymore problems. This works really well in the cryostat chamber, too! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Tue 12/15/2009 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Dispenser Thingy I have Feather dispensers with blades that I have to pry out, which is probably not a good thing. I've tried freezing the dispenser, putting it in an oven for a while and whacking the darned thing on the floor (not necessarily all at the same time..). Have we had this discussion before and have we solved this problem? Help? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= ------------------------------ Message: 3 Date: Tue, 15 Dec 2009 11:39:48 -0800 From: Maria Katleba Subject: [Histonet] RE: Blade Dispenser Thingy To: "Bonner, Janet" , "Breeden, Sara" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I use one drop of microtome oil and they slip right out. I mean don't go crazy with the oil either! Maria Katleba HT(ASCP) MS Napa CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Tuesday, December 15, 2009 10:51 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blade Dispenser Thingy I keep the dispenser in a small plastic bag that seals along with the desiccant packages they put in the original box with the blades, (and any other desiccant packages I can find - from coverslips, inks etc.) As long as you keep the blades sealed with the desiccant, you shouldn't have anymore problems. This works really well in the cryostat chamber, too! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Tue 12/15/2009 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Dispenser Thingy I have Feather dispensers with blades that I have to pry out, which is probably not a good thing. I've tried freezing the dispenser, putting it in an oven for a while and whacking the darned thing on the floor (not necessarily all at the same time..). Have we had this discussion before and have we solved this problem? Help? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ------------------------------ Message: 4 Date: Tue, 15 Dec 2009 12:05:00 -0800 From: Maria Katleba Subject: [Histonet] Histology Jobs Outside the USA To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Anyone know where you can find Histology positions OUTSIDE the USA? I have had several colleagues ask... and I am actually interested in this information as well..... Any thoughts? Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ------------------------------ Message: 5 Date: Tue, 15 Dec 2009 15:00:18 -0700 From: "Bell, Pat" Subject: [Histonet] COX2 in mice To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <64DB27005E2FD3439E88502D7A5C91218E0894A64B@CORTEZ.ucdenver.pvt> Content-Type: text/plain; charset="us-ascii" Does anyone know of a good antibody for COX2 that works in the mouse? Thank you, Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu ------------------------------ Message: 6 Date: Tue, 15 Dec 2009 17:23:00 -0500 From: "Rachel, Rivka (NIH/NEI) [E]" Subject: [Histonet] Anti-Flag antibody To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Does anyone know of a good rabbit anti-Flag antibody that can be used on transfected mouse cells? -- Rivka A. Rachel National Eye Institute ------------------------------ Message: 7 Date: Tue, 15 Dec 2009 16:21:12 -0700 From: "Liz Chlipala" Subject: [Histonet] Leica Bond To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hey everyone I have a question regarding the Leica Bond IHC stainer. We are currently evaluating it. Is it true that you can not use a protein block on the instrument and if you do you have to rinse afterwards. I know that a lot of labs do not use protein blocks anymore but we routinely use them here, we use a serum free protein block on all of our IHC slides. If there is anyone out there that is willing to give me some advice on this? Also if anyone wants to comment on the use or lack of use of protein block that would be greatly appreciated also. I'm beginning to think I may be old fashioned but I'm a bit concerned about not using protein block, OMG I hope I'm not turning into one of those techs who has been in the field so long that they have become resistant to change, yikes! Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 ------------------------------ Message: 8 Date: Tue, 15 Dec 2009 15:29:58 -0800 From: "Debrosse-Serra, Beatrice" Subject: RE: [Histonet] Leica Bond To: "Liz Chlipala" , Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Liz, We have Bonds and use protein blocks all the time. It isn't part of the kit, but you can use it in one of the open containers. Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, December 15, 2009 3:21 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Leica Bond Hey everyone I have a question regarding the Leica Bond IHC stainer. We are currently evaluating it. Is it true that you can not use a protein block on the instrument and if you do you have to rinse afterwards. I know that a lot of labs do not use protein blocks anymore but we routinely use them here, we use a serum free protein block on all of our IHC slides. If there is anyone out there that is willing to give me some advice on this? Also if anyone wants to comment on the use or lack of use of protein block that would be greatly appreciated also. I'm beginning to think I may be old fashioned but I'm a bit concerned about not using protein block, OMG I hope I'm not turning into one of those techs who has been in the field so long that they have become resistant to change, yikes! Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 16 Dec 2009 00:39:53 -0500 From: John Kiernan Subject: Re: [Histonet] FW: Notch-Nodal Paper To: "Margaryan, Naira" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; CHARSET=US-ASCII Dear Naira Margaryan, Please clarify "in vivo", which means "in alive ...". What is your "..."? Do you want to stain living cells in thin-layer tissue cultures, suspended cells (blood, haemolymph etc), or cells in whole organisms that are small enough to live under a coverslip (such as rotifers, little nematodes and protozoans)? For larger animals, in vivo microscopy is possible only at the surface (skin and other accessible surfaces) or where there are transparent media (the eye). Beware of adverts for fluorescent stains with catchy names! If you hope to be a career scientist you will run into the peer review process, which is severe and does not like results based on unexplained trade secrets. If you explain your question on histonet, you will get plenty of good advice, supplemented with peer-reviewed references that you can check. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Margaryan, Naira" Date: Tuesday, December 15, 2009 13:02 Subject: [Histonet] FW: Notch-Nodal Paper To: "histonet@lists.utsouthwestern.edu" > Hi histonetters, > > I am looking for some dye to be used in vivo like cell tracker > or similar might work for identifying live cells. > > Any suggestions are appreciated, > Naira > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 16 Dec 2009 08:20:29 -0800 From: "Jennifer Campbell" Subject: [Histonet] any info on Glutathione S-Transferase Pi (GST Pi)? To: Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF82FA3D1@VDXSERVER01.vdxpathology.local> Content-Type: text/plain; charset="us-ascii" Hi All, Has anyone ever used this antibody in FFPE mouse or rat tissue? How easy of an antibody is it to work with? Other comments? We may be doing this for an upcoming project and I just wanted to know what I could be getting myself into. Thanks! Jen Campbell ------------------------------ Message: 11 Date: Wed, 16 Dec 2009 12:03:29 -0500 From: "Yaskovich, Ruth A (NIH/NIDCR) [E]" Subject: [Histonet] Equipment Repair To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone know of a company that repairs equipment in the Maryland area? I'm in Bethesda and my old Tissue-Tek embedding center won't hold the temperature. Thanks for any help, Ruth Yaskovich National Institutes of Health Bethesda, Maryland ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 73, Issue 24 **************************************** From histonet.nospam <@t> vneubert.com Wed Dec 16 12:43:09 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Wed Dec 16 12:43:16 2009 Subject: [Histonet] leaving IHC slides in wash buffer In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF82FA3F0@VDXSERVER01.vdxpathology.local> References: <5658CBDB9EAE6545ABE50D2563D81BF82FA3F0@VDXSERVER01.vdxpathology.local> Message-ID: <4B292A3D.1050808@vneubert.com> I guess yes. Unless I can say exactly which ingredient will affect my staining, I would not leave them in any solution for such a long time! > Hi Everyone, > > I have yet another IHC-related question. So after antigen retrieval, > and then allowing my slides too cool, I place them in wash buffer before > loading them on to the autostainer. Would leaving the slides in the > wash buffer in the fridge for an extended period of time (say, 1 week) > before continuing with immunostaining affect staining quality in any > way? > > Thank you, > > Jen Campbell > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tp2 <@t> medicine.wisc.edu Wed Dec 16 12:44:25 2009 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Wed Dec 16 12:44:41 2009 Subject: [Histonet] restaining IHC slides Message-ID: <4B28D628020000DF0001EA8E@gwmail.medicine.wisc.edu> Hello Histonetters, I ran into an issue when staining some TMA slides the other day. This question isn't about how to fix the staining, or lack there of. I would like to know whether or not it is possible to repeat the same protocol on the same slides after removing the coverslips. If any of you have any experience with this sort of thing, please let me know. Tom Pier From SAllen <@t> exchange.hsc.mb.ca Wed Dec 16 12:52:16 2009 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Wed Dec 16 12:53:01 2009 Subject: [Histonet] good Calcafluor & Auramine/Rhodamine staining method for FFPE tissue Message-ID: Hi, I am posting this question for Lisa, Our Microbiology department would like to find a good calcafluor staining method and a good Auramine/Rhodamine staining method for FFPE tissues. Any suggestions? Would you be able to post this for me? Thanks, Lisa Lisa Manning MLT, BSc. Pathology Technical Director Diagnostic Services of Manitoba 401B Brodie Centre 727 McDermot Avenue Winnipeg, Manitoba R3E 3P5 Ph. (204) 789-3325 Fax (204) 789-3931 lmanning@hsc.mb.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From luke.perkocha <@t> ucsf.edu Wed Dec 16 12:57:03 2009 From: luke.perkocha <@t> ucsf.edu (Perkocha, Luke) Date: Wed Dec 16 12:57:08 2009 Subject: [Histonet] A good "lean" consultant for a pathology lab Message-ID: We are a multi-site academic AP-only laboratory and are looking for recommendations for process improvement consultants/trainers with experience with Lean, Six-sigma and other process improvement tools. We would like to bring someone in initially to help with a couple of small projects, with goals of: 1. Improving both process and physical environment to increase efficiency and reduce the chance of error (improve patient safety) in the EM lab. 2. Simultaneously providing some training, so that some tools and competencies are left over in the Department in this area. If this is a good experience, we would like to look at larger divisions (i.e. gross room, histology, specimen transport and receipt, professional sign-out, etc.) or more of the overall production process. There are so many consultants out there. Can anyone recommend someone or a group that is both competent and cost-effective, and can handle both the project itself, plus some training around it - hopefully with a track record of success in similar lab situations? Many thanks for your help! Luke Perkocha Luke.perkocha@ucsf.edu 415 885-7254 From jackdodo <@t> msn.com Wed Dec 16 13:19:13 2009 From: jackdodo <@t> msn.com (Jack Dodo) Date: Wed Dec 16 13:19:19 2009 Subject: [Histonet] VZV Controls Message-ID: Does anyone have VZV Controls? A block would be great, but at this point I will take anything. Can anyone point me in the right direction in finding this? Thanks _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/171222985/direct/01/ From carl.hobbs <@t> kcl.ac.uk Wed Dec 16 13:36:50 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Wed Dec 16 13:37:34 2009 Subject: [Histonet] Re: COX2 in mice Message-ID: <11D9615B89C10747B1C985966A63D7CA2C434CE8BD@KCL-MAIL04.kclad.ds.kcl.ac.uk> What are your experimental conditions? carl From Janice.Mahoney <@t> alegent.org Wed Dec 16 13:44:47 2009 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Dec 16 13:44:59 2009 Subject: [Histonet] RE: A good "lean" consultant for a pathology lab In-Reply-To: References: Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A54E6@EXCHMBC2.ad.ah.local> I'd highly recommend Randy Stephens. randystephens@mac.com Jan Mahoney, Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perkocha, Luke Sent: Wednesday, December 16, 2009 12:57 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] A good "lean" consultant for a pathology lab We are a multi-site academic AP-only laboratory and are looking for recommendations for process improvement consultants/trainers with experience with Lean, Six-sigma and other process improvement tools. We would like to bring someone in initially to help with a couple of small projects, with goals of: 1. Improving both process and physical environment to increase efficiency and reduce the chance of error (improve patient safety) in the EM lab. 2. Simultaneously providing some training, so that some tools and competencies are left over in the Department in this area. If this is a good experience, we would like to look at larger divisions (i.e. gross room, histology, specimen transport and receipt, professional sign-out, etc.) or more of the overall production process. There are so many consultants out there. Can anyone recommend someone or a group that is both competent and cost-effective, and can handle both the project itself, plus some training around it - hopefully with a track record of success in similar lab situations? Many thanks for your help! Luke Perkocha Luke.perkocha@ucsf.edu 415 885-7254 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From CIngles <@t> uwhealth.org Wed Dec 16 13:47:35 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Dec 16 13:47:46 2009 Subject: [Histonet] FW: FW: Follow Up: Joint Commission Lab Survey CorrectiveAction Plan West MOHS References: <4B288661.BFFC.00C7.0@UWMF.WISC.EDU> Message-ID: OK Guys, here's another JCAHO/CLIA controversy. Has anyone (Mostly MOHS labs) been sited for this? Currently we only save tissue specimens 24 hours in case the Dr. decides to send some to path after the fact. (don't get me going on morphology quality). Does anyone have a clear definition of "gross tissue specimens" It's not like we measure this stuff before freezing. Thanks for your well educated guesses Claire West MOHS Clinic was cited for a deficiency regarding maintenance and storage of tissue during the Joint Commission Lab Survey. According to standard QC.2.120, EP 2: Microscopic slides, paraffin blocks, bone marrow aspirates, needle biopsy specimens, and gross tissue specimens are stored for required times as defined by organization policy, law, and regulation. Microscopic slides, including stained slides, are retained for at least 10 years. Paraffin blocks are stored for a minimum of two years from the date of examination. Gross tissue specimens are retained for at least seven days after required microscopic sections are examined and reports reviewed and signed. State law and regulation requirements are for longer times (See Appendix XX for additional specific retention times.) The surveyor observed in his report that "biopsy tissue was frozen for section, slides prepared and then the tissue was discarded. Gross tissue specimens must be retained for seven days after the microscopic sections are examined and reports reviewed and signed." From rjbuesa <@t> yahoo.com Wed Dec 16 14:42:49 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 16 14:42:53 2009 Subject: [Histonet] leaving IHC slides in wash buffer In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF82FA3F0@VDXSERVER01.vdxpathology.local> References: <5658CBDB9EAE6545ABE50D2563D81BF82FA3F0@VDXSERVER01.vdxpathology.local> Message-ID: <828678.45329.qm@web65710.mail.ac4.yahoo.com> I would never do that. Why subject your sections to such a treatment? Ren? J. ________________________________ From: Jennifer Campbell To: histonet@lists.utsouthwestern.edu Sent: Wed, December 16, 2009 1:34:40 PM Subject: [Histonet] leaving IHC slides in wash buffer Hi Everyone, ? I have yet another IHC-related question.? So after antigen retrieval, and then allowing my slides too cool, I place them in wash buffer before loading them on to the autostainer.? Would leaving the slides in the wash buffer in the fridge for an extended period of time (say, 1 week) before continuing with immunostaining affect staining quality in any way? Thank you, Jen Campbell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From milton.gomez <@t> aruplab.com Wed Dec 16 14:48:51 2009 From: milton.gomez <@t> aruplab.com (Gomez, Milton) Date: Wed Dec 16 14:49:07 2009 Subject: [Histonet] immunoperoxidase background staining Message-ID: <6995F66A2196514DB0C4CAA5A32EA2938E533F@postoffice01.aruplab.net> Dear Histonetters, I am getting background staining with my Immunohistochemistry protocols that require CC1 and CC2. I am thinking of adding AB block to the protocols. Do you have any other suggestions?. I use the ULTRA stainers. Thank you very much in advance, Milton A. Gomez, HTL (ASCP) Technical Supervisor Immunohistochemistry Department ARUP Laboratories, Inc. 500 Chipeta Way Salt Lake City, UT 84108-1221 Desk Phone: 801-583-2787, ext.3869 Lab. Phone: 801-584-5257/5242 Fax: 801-584-5217 E-mail: milton.gomez@aruplab.com Web: www.aruplab.com - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From ogv_histech <@t> yahoo.com Wed Dec 16 14:59:21 2009 From: ogv_histech <@t> yahoo.com (oscar gonzalez) Date: Wed Dec 16 14:59:24 2009 Subject: [Histonet] IMAGING Message-ID: <327904.86285.qm@web53506.mail.re2.yahoo.com> If you have seen the new CAP inspection lists, there are several questions in regards to Imaging. Does anybody have a procedure to cover such questions? Thanks. From tkngflght <@t> yahoo.com Wed Dec 16 16:08:00 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Dec 16 16:08:04 2009 Subject: [Histonet] looking for KOH nail procedure Message-ID: <791706.69487.qm@web50901.mail.re2.yahoo.com> ?Hi Guys! ? I've used a potassium hydroxide solution to 'soften' nails after fixation but before processing.? I cannot find my procedure!! ? Can anyone help? ? Cheryl Kerry, HT(ASCP) ? From AnthonyH <@t> chw.edu.au Wed Dec 16 16:15:32 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Dec 16 16:15:44 2009 Subject: [Histonet] FNA stain In-Reply-To: Message-ID: We use the Diff quik (or whatever spelling you use!)stain on air-dried smears. There is also a rapid Poor man's H&E stain, using the Diff Quik solutions, that you can also use. You can then restain with the normal PAP stain back in the lab (Hirschowitz et al Acta Cytolog (1994) 38:499-501) We try to give an immediate provisional diagnosis at the time Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DKBoyd@chs.net Sent: Thursday, 17 December 2009 5:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FNA stain This question is for those of you who perform fine needle aspirations. What stain are you using for your immediate evaluation? Or do you give an immediate evaluation/adequacy? Thanks. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net ------------------------------------------------------------------------ -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From milton.gomez <@t> aruplab.com Wed Dec 16 16:26:33 2009 From: milton.gomez <@t> aruplab.com (Gomez, Milton) Date: Wed Dec 16 16:27:59 2009 Subject: [Histonet] immunoperoxidase background staining References: <6995F66A2196514DB0C4CAA5A32EA2938E533F@postoffice01.aruplab.net> Message-ID: <6995F66A2196514DB0C4CAA5A32EA2938E5342@postoffice01.aruplab.net> These are brand new installs. it does it only for the CC1 and CC2 Protocols. Milton A. Gomez, HTL (ASCP) Technical Supervisor Immunohistochemistry Department ARUP Laboratories, Inc. 500 Chipeta Way Salt Lake City, UT 84108-1221 Desk Phone: 801-583-2787, ext.3869 Lab. Phone: 801-584-5257/5242 Fax: 801-584-5217 E-mail: milton.gomez@aruplab.com Web: www.aruplab.com ________________________________ From: Jackie Smith [mailto:roosmith1@hotmail.com] Sent: Wed 12/16/2009 3:24 PM To: Gomez, Milton Subject: RE: [Histonet] immunoperoxidase background staining When was the last decontamination performed? Is it all of your protocols? > Date: Wed, 16 Dec 2009 13:48:51 -0700 > From: milton.gomez@aruplab.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] immunoperoxidase background staining > > Dear Histonetters, > > I am getting background staining with my Immunohistochemistry protocols that require CC1 and CC2. I am thinking of adding AB block to the protocols. Do you have any other suggestions?. I use the ULTRA stainers. > > Thank you very much in advance, > > Milton A. Gomez, HTL (ASCP) > Technical Supervisor > Immunohistochemistry Department > ARUP Laboratories, Inc. > 500 Chipeta Way > Salt Lake City, UT 84108-1221 > Desk Phone: 801-583-2787, ext.3869 > Lab. Phone: 801-584-5257/5242 > Fax: 801-584-5217 > E-mail: milton.gomez@aruplab.com > Web: www.aruplab.com > > - ------------------------------------------------------------------ > The information transmitted by this e-mail and any included > attachments are from ARUP Laboratories and are intended only for the > recipient. The information contained in this message is confidential > and may constitute inside or non-public information under > international, federal, or state securities laws, or protected health > information and is intended only for the use of the recipient. > Unauthorized forwarding, printing, copying, distributing, or use of > such information is strictly prohibited and may be unlawful. If you > are not the intended recipient, please promptly delete this e-mail > and notify the sender of the delivery error or you may call ARUP > Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 > (800) 522-2787 ext. 2100 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Hotmail: Trusted email with Microsoft's powerful SPAM protection. Sign up now. - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From NickD <@t> alleninstitute.org Wed Dec 16 17:24:17 2009 From: NickD <@t> alleninstitute.org (Nick Dee) Date: Wed Dec 16 17:24:33 2009 Subject: [Histonet] RE: Bubbles on Fresh Frozen tissue after IHC In-Reply-To: References: Message-ID: Hi- I am doing immunohistochemistry on fresh frozen tissue using the capillary gap method on Tecans. The preferred fixative for my antigen is a cold acetone fix. I am using MeOH/H2O2 to kill peroxidase before the blocking step and it's resulting in many small bubbles forming on the tissue in which I get no staining...in the area where there aren't bubbles, the staining looks great. I am aware of using something other than MeOH to block for peroxidase, however when I don't use an alcohol, the surface tension pulls all the tissue off my slide when I'm taking apart the capillary gap chambers to coverslip. Has anyone experienced this before? Any ideas/suggestions would be appreciated. Thank you, Nick From renafail <@t> bellsouth.net Wed Dec 16 17:28:30 2009 From: renafail <@t> bellsouth.net (Rena Fail) Date: Wed Dec 16 17:28:34 2009 Subject: [Histonet] restaining IHC slides In-Reply-To: <4B28D628020000DF0001EA8E@gwmail.medicine.wisc.edu> References: <4B28D628020000DF0001EA8E@gwmail.medicine.wisc.edu> Message-ID: <952884.40384.qm@web180301.mail.gq1.yahoo.com> you can use the same slides , repeating all the steps except ?the antigen retrieval ( if it is the same ) Ideally you should use new slides, but on tiny bxs, outside slides, or very limited area of interest,?that is not aways possible. Rena Fail? ----- Original Message ---- From: Thomas Pier To: histonet@lists.utsouthwestern.edu Sent: Wed, December 16, 2009 1:44:25 PM Subject: [Histonet] restaining IHC slides Hello Histonetters, I ran into an issue when staining some TMA slides the other day. This question isn't about how to fix the staining, or lack there of. I would like to know whether or not it is possible to repeat the same protocol on the same slides after removing the coverslips. If any of you have any experience with this sort of thing, please let me know. Tom Pier _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Dec 16 19:12:15 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Dec 16 19:12:30 2009 Subject: [Histonet] looking for KOH nail procedure In-Reply-To: <791706.69487.qm@web50901.mail.re2.yahoo.com> References: <791706.69487.qm@web50901.mail.re2.yahoo.com> Message-ID: we use 20% sodium hydroxide. Works great ----- Original Message ----- From: "Cheryl" To: Sent: Wednesday, December 16, 2009 4:08 PM Subject: [Histonet] looking for KOH nail procedure Hi Guys! I've used a potassium hydroxide solution to 'soften' nails after fixation but before processing. I cannot find my procedure!! Can anyone help? Cheryl Kerry, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Dec 16 19:34:29 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Dec 16 19:34:37 2009 Subject: [Histonet] Re: FNA stain Message-ID: Debbie M. Boyd, HT(ASCP), Chief Histologist, Southside Regional Medical Center in Petersburg, Virginia asks: >>This question is for those of you who perform fine needle aspirations. What stain are you using for your immediate evaluation? Or do you give an immediate evaluation/adequacy?<< This is a really good question that demands a careful answer. You need to do what your cytotechnologist and your pathologist are comfortable with. Air-dried smears stained with a rapid azure-eosin (Diff-Quik and generic equivalents) stain are simple and fast - IF your pathologist has been trained to interpret them, which I (I'm 70 years old, mind you) frankly wasn't. I can live with a good H and E stain - a full-dress Pap stain is nice, but probably takes too long to do. The cytotechnologist and pathologist certainly have to give an immediate decision as to the adequacy of the specimen. There's a CPT code for that, even. And sometimes what's needed is a flat-out diagnosis. But this is something that needs to be worked out in advance - not under the stress of a high-pressure clinical situation, but comfortably, with pizza or worse. Bob Richmond Samurai Pathologist Knoxville TN From ree3 <@t> leicester.ac.uk Thu Dec 17 03:27:05 2009 From: ree3 <@t> leicester.ac.uk (Edwards, Richard E.) Date: Thu Dec 17 03:27:53 2009 Subject: [Histonet] RE: leaving IHC slides in wash buffer In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF82FA3F0@VDXSERVER01.vdxpathology.local> References: <5658CBDB9EAE6545ABE50D2563D81BF82FA3F0@VDXSERVER01.vdxpathology.local> Message-ID: <7722595275A4DD4FA225B92CDBF174A1E8C9F7E03E@EXC-MBX3.cfs.le.ac.uk> Why?, and why introduce such an apparently unnecessary variable?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: 16 December 2009 18:35 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] leaving IHC slides in wash buffer Hi Everyone, I have yet another IHC-related question. So after antigen retrieval, and then allowing my slides too cool, I place them in wash buffer before loading them on to the autostainer. Would leaving the slides in the wash buffer in the fridge for an extended period of time (say, 1 week) before continuing with immunostaining affect staining quality in any way? Thank you, Jen Campbell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Thu Dec 17 05:06:23 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Thu Dec 17 05:07:14 2009 Subject: [Histonet] RE: leaving IHC slides in wash buffer In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1E8C9F7E03E@EXC-MBX3.cfs.le.ac.uk> References: <5658CBDB9EAE6545ABE50D2563D81BF82FA3F0@VDXSERVER01.vdxpathology.local> <7722595275A4DD4FA225B92CDBF174A1E8C9F7E03E@EXC-MBX3.cfs.le.ac.uk> Message-ID: The rule is buffer no longer than 1 hour... so it will definitely compromise your IHC stain where the antibody may already be marginal. Sorry.... Maria Katleba HT(ASCP) MS Napa Ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Thursday, December 17, 2009 1:27 AM To: 'Jennifer Campbell'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: leaving IHC slides in wash buffer Why?, and why introduce such an apparently unnecessary variable?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: 16 December 2009 18:35 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] leaving IHC slides in wash buffer Hi Everyone, I have yet another IHC-related question. So after antigen retrieval, and then allowing my slides too cool, I place them in wash buffer before loading them on to the autostainer. Would leaving the slides in the wash buffer in the fridge for an extended period of time (say, 1 week) before continuing with immunostaining affect staining quality in any way? Thank you, Jen Campbell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From sheila_adey <@t> hotmail.com Thu Dec 17 05:15:17 2009 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Dec 17 05:15:21 2009 Subject: [Histonet] Silver Nitrate instead of inking?? Message-ID: Hi Everyone, Has anyone ever heard of using Silver Nitrate for inking skin bx's before processing??? One of our paths mentioned it. Thanks in advance. Sheila Adey HT MLT _________________________________________________________________ Eligible CDN College & University students can upgrade to Windows 7 before Jan 3 for only $39.99. Upgrade now! http://go.microsoft.com/?linkid=9691819 From rjbuesa <@t> yahoo.com Thu Dec 17 07:57:39 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 17 07:57:45 2009 Subject: [Histonet] Silver Nitrate instead of inking?? In-Reply-To: References: Message-ID: <675814.78673.qm@web65706.mail.ac4.yahoo.com> Silver nitrate will react in the skin and transform in silver chloride BUT in order to get black it needs to be exposed to solar or very strong light, and the reaction is not instantaneous. Your PT seems that at some point in his/her life s/he got silver nitrate in the skin that transformed into a black stain. but that will not readily "fly" in a lab setting. Ren? J. ________________________________ From: sheila adey To: histonet@lists.utsouthwestern.edu Sent: Thu, December 17, 2009 6:15:17 AM Subject: [Histonet] Silver Nitrate instead of inking?? Hi Everyone, Has anyone ever heard of using Silver Nitrate for inking skin bx's before processing??? One of our paths mentioned it. Thanks in advance. Sheila Adey HT MLT ??? ??? ??? ? ??? ??? ? _________________________________________________________________ Eligible CDN College & University students can upgrade to Windows 7 before Jan 3 for only $39.99. Upgrade now! http://go.microsoft.com/?linkid=9691819_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bliven.laura <@t> marshfieldclinic.org Thu Dec 17 08:22:58 2009 From: bliven.laura <@t> marshfieldclinic.org (Bliven, Laura) Date: Thu Dec 17 08:23:09 2009 Subject: [Histonet] IHC marker RANKL protein Message-ID: <200912171422.nBHEMxhZ006704@mailhost2.mfldclin.edu> I am looking for a reference lab that will stain and interpret the IHC marker RANKL. Any information would be appreciated. Thanks, Laura bliven.laura@marshfieldclinic.org Laura Bliven, AAS, HT(ASCP), QIHC Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From wlecorch <@t> rwjuhh.edu Thu Dec 17 08:29:48 2009 From: wlecorch <@t> rwjuhh.edu (Lecorchick, William) Date: Thu Dec 17 08:29:57 2009 Subject: [Histonet] RE: FNA stain In-Reply-To: <1261010752.701068@messagescreen2.rwjham.net> References: <1261010752.701068@messagescreen2.rwjham.net> Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71A4AB78AB3@HAMEXMBA.rwjham.local> Here we provide onsite adequacy for every FNA. The normal is for every pass preformed we make two smears one is air dried and stained using Diff-Quick, and the other is fixed in 95% ETOH using Pap or H&E.It's a fast and easy to do bedside in cases with bilateral nodules as the Radiologist is switching from the left to the right side of the bed we can stain the first nodule before he has the second started. From LSebree <@t> uwhealth.org Thu Dec 17 09:13:40 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Dec 17 09:13:49 2009 Subject: [Histonet] hsNFS(INI1) antibody Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF6E7@UWHC-MAIL01.uwhis.hosp.wisc.edu> Hi, I'm looking for a reference lab that performs an IHC stain for this antigen. Its used for diagnosing malignant rhabdoid tumor where this antigen is lost. It is expressed in normal tissue. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From Ronald.Houston <@t> nationwidechildrens.org Thu Dec 17 09:21:39 2009 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Dec 17 09:22:01 2009 Subject: [Histonet] hsNFS(INI1) antibody In-Reply-To: <8C023B4AB999614BA4791BAEB26E27381BF6E7@UWHC-MAIL01.uwhis.hosp.wisc.edu> References: <8C023B4AB999614BA4791BAEB26E27381BF6E7@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21B645EA9@chi2k3ms01.columbuschildrens.net> Cell Marque, clone MRQ-27, gives excellent results on the BondMax after EDTA retrieval. Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, December 17, 2009 10:14 AM To: Histonet Subject: [Histonet] hsNFS(INI1) antibody Hi, I'm looking for a reference lab that performs an IHC stain for this antigen. Its used for diagnosing malignant rhabdoid tumor where this antigen is lost. It is expressed in normal tissue. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From jonesly <@t> mir.wustl.edu Thu Dec 17 10:21:11 2009 From: jonesly <@t> mir.wustl.edu (Jones, Lynne) Date: Thu Dec 17 10:21:19 2009 Subject: [Histonet] Lab chair Message-ID: <208330218E165F45AB6307B787F5EA473A71516567@RAD-VMSRVEXV2.rad.wustl.edu> Hello - I'm a bit late replying (and can't locate the e-mail address of the OP), but I hope the information below is useful. I have rather strong opinions on this subject :) Our technicians can easily spend 6-8 hours seated at the bench, and after 15 years at the bench myself, I understand that proper seating (and lighting and instruments) is a necessity, not a luxury. When we moved into a new facility, I wanted ergonomic chairs that were durable, (relatively) solvent resistant and easily cleaned. We work with biological material, solvents and radioactivity, and often perform hours of gross dissection. I had used several styles of Safco seating in another facility and even after 7 years they showed minimal wear. Similar but less expensive lab stools/chairs are sold by some of the large science and office supply companies and with whom we have contracts, so I had to plead my case that the Safco chairs I selected were not an extravagance. (Office and lab seating was in the process of being standardized as a cost-saving measure, and so it was an opportune time for input from folks in the lab.) Several vendors provided demo chairs and I brought over a Safco chair and stool as well as one of the less expensive lab chairs to illustrate typical wear. (When the outer skin wears off the less expensive foam chairs, from physical wear and exposure to cleaning solvents, they become more porous and removal of radioactive contamination is more challenging.) Everybody got to try out all the chairs, and we talked about the pros and cons of specific features, and also the challenge of finding chairs to fit employees with varying proportions. We also discussed features that should be avoided for lab seating - like mesh backs in any lab that works with blood or biohazards. (Some vendors don't seem to understand the difference between office and lab seating.) You may find other models that fit your needs, but in my experience, adjustable backs and seats that tilt to adjust significantly ease the inevitable fatigue that comes from sitting for >4 hours as do foot rings, while fixed armrests set an improper height can increase strain. I have no affiliation with the Safco company, but have been very impresses with the SitStar stool series and the Taskmaster 5113 seating. Here is the website: http://www.safcoproducts.com/ SitStar 6660 stools (all with optional backs). SitStar 6659 stools (one with no back, two with backs). Optional SitStar 6661 backs to go with the stools. Taskmaster 5113 chairs (two with arm-rests). Optional Taskmaster 5144 T-Bar Armrests to go with the chairs. My views are my personal opinions and don't represent my employer, but without ergonomic seating, I know my own productivity would be reduced. Lynne Jones > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Manalac, Rosa > Sent: Mon 12/14/2009 12:24 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Lab chair > > > > Can anyone suggest a comfortable and ergonomic chair to use while > working with the microtome for many hours each day? I would > appreciate > information on the brand, model, or type and where to order it. > Even a > website would help. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ======================================================= > The information contained in this message may be privileged and/or > confidential > and protected from disclosure. If the reader of this message is > not the intended > recipient or an employee or agent responsible for delivering this > message to the > intended recipient, you are hereby notified that any dissemination, > distribution > or copying of this communication is strictly prohibited. If you > have received this > communication in error, please notify the sender immediately by > replying to this > message and deleting the material from any computer. > ======================================================= > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 14 Dec 2009 15:36:22 -0500 From: "Bonner, Janet" Subject: RE: [Histonet] Lab chair To: "Akemi Allison" Cc: histonet@lists.utsouthwestern.edu Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2C00@fhosxchmb006.ADVENTISTCORP.NET> Content-Type: text/plain; charset=iso-8859-1 The chairs have to have vinyl seats and back supports, not cloth, so they can be easily washed. Janet ________________________________ From: Akemi Allison [mailto:akemiat3377@yahoo.com] Sent: Mon 12/14/2009 1:36 PM To: Bonner, Janet Cc: Manalac, Rosa; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Lab chair Whatever chair you choose, make sure you meet you lab safety requirements. In one of the lab's I supervised, we were dinged for not having chairs with (5) verses (4) legs when we went through an inspection. We had to replace all our lab chairs to meet the required standards. I am not sure if this is a set standard, or it varies from State to State. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3377@yahoo.com On Dec 14, 2009, at 11:23 AM, Bonner, Janet wrote: We had a great company that used distributors and right after we bought the workchairs, they went somewhere else! Google Ergonomic workchair and you should get a good selection. Janet ________________________________________ The material in this message is private and may contain Protected Healthcare Information (PHI). If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= The material in this message is private and may contain Protected Healthcare Information (PHI). If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. From Joyce.Cline <@t> wchsys.org Thu Dec 17 10:37:15 2009 From: Joyce.Cline <@t> wchsys.org (Joyce Cline) Date: Thu Dec 17 10:37:19 2009 Subject: [Histonet] ThermoScientific stainers Message-ID: Does anyone have the Gemini ES stainer and the Clearvue cloverslipper? How is the daily performance? Problems? Thank you Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From relia1 <@t> earthlink.net Thu Dec 17 10:54:04 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Dec 17 10:54:16 2009 Subject: [Histonet] RELIA Histology Job Alert and a last minute shopping tip! Message-ID: Hi Histonetters!! I hope everybody is having a great day! I have a last minute shopping tip for you... Today is National Free Shipping Day so if you have any online shopping left to do here is the website: www.freeshippingday.com Also I wanted to give you a heads up on my current openings. All of my clients offer excellent compensation benefits and relocation assistance. They are willing to interview right away or after the holidays whichever is more convenient for you. Here is a list of my current openings: HISTOLOGY/PATHOLOGY MANAGEMENT WI ? Waukesha AP Team Leader NY- Orange/Rockland County Histology Supervisor KS ? Topeka AP Manager MA ? Cape Cod Pathology Supervisor NC ? Charlotte Histology Manager OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital CA ? Central CA ? Pathology Supervisor CA ? Los Angeles ?Histology Supervisor HISTOTECHS LA ? Lead IHC Tech ?private lab FL ? Pensacola- Histotech private lab MI ? Jackson ? Grossing Histotechnologist HTL req, Private lab TX ? Austin ?Night Shift Histotech TX ? Fort Worth MOHS Tech part time MA ? North Shore of Boston ? Histotech 2nd or 3rd shift NY-Orange/Rockand County-Brand New Lab NYS license req NY-Upstate NY-NYS license req NY-NYC-night shift NYS license req FL- Largo-part time FL lic req Pathology Assistant NC ? Charlotte PA grad from NAACLES program required If you or anyone you know might be interested in any of these opportunities please contact me. Thanks-Pam Happy Holidays!! Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net < mailto:relia1@earthlink.net> << http://home.earthlink.net/~relia1>> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From Kim.Donadio <@t> bhcpns.org Thu Dec 17 10:57:16 2009 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Thu Dec 17 10:57:33 2009 Subject: [Histonet] New processor help In-Reply-To: <4B28B29B020000F200003CCB@mail.valleycare.com> Message-ID: Hi Carol, I am not aware of any procedure that is regulated for this. When we have processor problems or purchase a new one we usually run test on each specific protocol. ie: bx's, fat run etc. We just make up some blocks from scrap specimens and run them through. We log the outcome in our QA logs. Hope this helps Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Carole Ruffin" Sent by: histonet-bounces@lists.utsouthwestern.edu 12/16/2009 12:12 PM To cc Subject [Histonet] New processor help Is there a procedure or some standard method validation process out there for starting up a new tissue processor? Maybe one that would be approved by regulatory agency? I am not sure whether this would just be left up to the pathologists to decide what they would prefer. Thank you, Carole Ruffin, HT(ASCP) ValleyCare Hospital Pleasanton, CA This message and any included attachments are from ValleyCare Health System and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From rjbuesa <@t> yahoo.com Thu Dec 17 11:05:08 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 17 11:05:13 2009 Subject: [Histonet] New processor help In-Reply-To: Message-ID: <165593.65685.qm@web65701.mail.ac4.yahoo.com> And I think that is what everybody do and I?also consider?it is more than enough. Just make sure that the OK is given by the pathologist and that you keep the test slides in file and all the paperwork handy for any inspection. Another thing: do it but do not offer the information to any inspector. Just be prepared in case you are asked. Ren? J. --- On Thu, 12/17/09, Kim.Donadio@bhcpns.org wrote: From: Kim.Donadio@bhcpns.org Subject: Re: [Histonet] New processor help To: "Carole Ruffin" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Thursday, December 17, 2009, 11:57 AM Hi Carol, ? ? ? ? ? ? ? ? ???I am not aware of any procedure that is regulated for this. When we have processor problems or purchase a new one we usually run test on each specific protocol. ie: bx's, fat run etc. We just make up some blocks from scrap specimens and run them through. We log the outcome in our QA logs. Hope this helps Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Carole Ruffin" Sent by: histonet-bounces@lists.utsouthwestern.edu 12/16/2009 12:12 PM To cc Subject [Histonet] New processor help Is there a procedure or some standard method validation process out there for starting up a new tissue processor? Maybe one that would be approved by regulatory agency? I am not sure whether this would just be left up to the pathologists to decide what they would prefer. Thank you, Carole Ruffin, HT(ASCP) ValleyCare Hospital Pleasanton, CA This message and any included attachments are from ValleyCare Health System and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message.? For questions, contact the BHC Privacy Officer at (850) 434-4472.? Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu Dec 17 11:42:29 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Dec 17 11:42:39 2009 Subject: [Histonet] Staffing for IHC Message-ID: <4B2A2736.2B7F.00C9.0@geisinger.edu> I'm hoping some of the larger hospitals out there will share their data with me. I'm curious as to how labs are staffed for doing IF/IHC/CISH. A small portion of our staining protocols still require pretreatment by hand, but we run instruments that do the pretreatments on board for the majority of our stains. We have 2 IHC techs, 1 on 1st shift and 1 on 3rd shift.Together they are cutting and staining between 200-350 slides per day. I think this is a high volume for only two people. What do you all think? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From shive003 <@t> umn.edu Thu Dec 17 11:43:12 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Dec 17 11:43:16 2009 Subject: [Histonet] veterinary lab question - chem expiration Message-ID: <4B57386DCA574F80B21D58AE8E9CC5E3@auxs.umn.edu> What are veterinary labs (non-CAP inspected labs) setting as their expiration dates for dry and liquid chemicals that come without expiration dates on the labels? On the Histonet I've found varying answers from human labs (5-10 yrs for dry; 1-5 yrs for liquid). I'm in the process of setting guidelines for my labs, but want to be sure to be in agreement with other vet labs out there as well. Thanks, Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory From MLunetta <@t> luhcares.org Thu Dec 17 11:49:44 2009 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Thu Dec 17 11:49:56 2009 Subject: [Histonet] Re: FNA stain Message-ID: <4B2A0CC9020000A80003C1EF@gw4.luh.luhcares.org> Debbie, We use the Diff-Quick stain for immediate evaluation. Matt Lunetta HT (ASCP) Longmont United Hospital Longmont Colorado Message: 2 Date: Wed, 16 Dec 2009 13:24:10 -0500 From: DKBoyd@chs.net Subject: [Histonet] FNA stain To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" This question is for those of you who perform fine needle aspirations. What stain are you using for your immediate evaluation? Or do you give an immediate evaluation/adequacy? Thanks. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net From foreightl <@t> gmail.com Thu Dec 17 12:02:19 2009 From: foreightl <@t> gmail.com (Pat Laurie) Date: Thu Dec 17 12:02:26 2009 Subject: [Histonet] Staffing for IHC In-Reply-To: <4B2A2736.2B7F.00C9.0@geisinger.edu> References: <4B2A2736.2B7F.00C9.0@geisinger.edu> Message-ID: Angela, We do about the same number of IHC slides. We run primarily Ventana machines (one of the main benefits are reduced tech time) and are expanding into the intellipath. Our IHC department is relatively large, we have dedicated 1 person for leading and development, one person on both 1st and 2nd shift running the IHC as well as 1 person who will assist as needed on both shifts. Our IF and ISH are done on a seperate bench. We still have days where our IHC bench is overwhelmed even with as many people as we have. It seems that IHC volume per case in all institutions is going up as more tests are being developed. Patrick Laurie HT(ASCP)QIHC Histology Supervisor CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 On Thu, Dec 17, 2009 at 9:42 AM, Angela Bitting wrote: > I'm hoping some of the larger hospitals out there will share their data > with me. > I'm curious as to how labs are staffed for doing IF/IHC/CISH. > A small portion of our staining protocols still require pretreatment by > hand, but we run instruments that do the pretreatments on board for the > majority of our stains. > We have 2 IHC techs, 1 on 1st shift and 1 on 3rd shift.Together they are > cutting and staining between 200-350 slides per day. I think this is a high > volume for only two people. What do you all think? > > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. It is > intended solely for the addressee. Access to this message by anyone else is > unauthorized. If you are not the intended recipient, any disclosure, > copying, distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you have received this > message in error, please delete all electronic copies of this message (and > the documents attached to it, if any), destroy any hard copies you may have > created and notify me immediately by replying to this email. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plaurie@cellnetix.com From LSebree <@t> uwhealth.org Thu Dec 17 12:02:56 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Dec 17 12:03:00 2009 Subject: [Histonet] Staffing for IHC In-Reply-To: <4B2A2736.2B7F.00C9.0@geisinger.edu> Message-ID: <8C023B4AB999614BA4791BAEB26E27381BF6ED@UWHC-MAIL01.uwhis.hosp.wisc.edu> Your " I think this is a high volume for only two people" is an understatement! We have two techs also and cut/stain anywhere from 20 - 150 slides/day on a 7:30 - 4 pm shift and there are times when we're pulling our hair out. Your techs must be completely over the edge; get them some help. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, December 17, 2009 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staffing for IHC I'm hoping some of the larger hospitals out there will share their data with me. I'm curious as to how labs are staffed for doing IF/IHC/CISH. A small portion of our staining protocols still require pretreatment by hand, but we run instruments that do the pretreatments on board for the majority of our stains. We have 2 IHC techs, 1 on 1st shift and 1 on 3rd shift.Together they are cutting and staining between 200-350 slides per day. I think this is a high volume for only two people. What do you all think? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Dec 17 12:09:47 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Dec 17 12:12:53 2009 Subject: [Histonet] Staffing for IHC In-Reply-To: <4B2A2736.2B7F.00C9.0@geisinger.edu> References: <4B2A2736.2B7F.00C9.0@geisinger.edu> Message-ID: I have 2 full time techs (one of those techs is supposed to be devoted to research), one part time tech that cuts controls and one part time tech on the overnight shift to load the machines. I am supposed to only be at the bench 20% of the time, but it is much more, trying to cover vacations and sick time. I also help out on the research end and we have lots of it. There are times when we are extremely overwhelmed with IHC's and ISH/CISH We do about 35K IHC a year, not including the research slides. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting [akbitting@geisinger.edu] Sent: Thursday, December 17, 2009 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staffing for IHC I'm hoping some of the larger hospitals out there will share their data with me. I'm curious as to how labs are staffed for doing IF/IHC/CISH. A small portion of our staining protocols still require pretreatment by hand, but we run instruments that do the pretreatments on board for the majority of our stains. We have 2 IHC techs, 1 on 1st shift and 1 on 3rd shift.Together they are cutting and staining between 200-350 slides per day. I think this is a high volume for only two people. What do you all think? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Thu Dec 17 12:35:43 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Thu Dec 17 12:35:56 2009 Subject: [Histonet] Re: Bubbles on Fresh Frozen tissue after IHC Message-ID: Hi Nick, Do your peroxidase blocking offline in a coplin jar or staining bucket, prior to loading them in the capillary gap holder. Problem solved. Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO From valerio2 <@t> buffalo.edu Thu Dec 17 12:47:29 2009 From: valerio2 <@t> buffalo.edu (Mike Valerio) Date: Thu Dec 17 12:47:33 2009 Subject: [Histonet] Can anyone help me? Message-ID: <45038.1261075649@buffalo.edu> Hello, I would like to stain FFPE mouse brain tissue with MCP-1 antibody from serotec. I was wondering if anyone who uses this marker could share their protocol and indicate what is a good positive control. Thanks in advance. Michael Valerio University at Buffalo valerio2@buffalo.edu 716-829-5650 From Marilyn.A.Weiss <@t> kp.org Thu Dec 17 12:57:23 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Thu Dec 17 12:57:30 2009 Subject: [Histonet] histotechs grossing Message-ID: Can you please tell me if there are any HT's or HTL's grossing complex cases. If so , are they trained by their Pathologist or a school? Please e-mail me direct. Thank you. Marilyn.A.Weiss@kp.org. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From kenneth.a.troutman <@t> Vanderbilt.Edu Thu Dec 17 13:37:18 2009 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Thu Dec 17 13:37:23 2009 Subject: [Histonet] RE: immunoperoxidase background staining Message-ID: <7B310892042DA74CB3590053F424CFE60AFE62D09B@ITS-HCWNEM06.ds.Vanderbilt.edu> A couple of suggestions. First, what kind of fixative are you using? If you are using a heavy metal fixative, sometimes that can be the reason. We have had this issue with B-plus fixative (B5 replacement) and I have seen it with some protocols in zinc formalin and non-buffered fixatives. Second, try backing off of the CC1 or 2. I use a Biocare's Background Terminator in an option container to help with my most troublesome antibodies. You can also use the green diluent they provide you with as a blocking reagent. I found it works on most things. If you are having issues with tons of background on ALL your slides, I would send a slide to a place that has established protocols to see if it is the instrument or the tissue. Hope this helps. Ashley Troutman BS, HT(ASCP) QIHC Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4532 Nashville, TN 37232 615-343-9134 From silvinamolinuevo <@t> yahoo.com.ar Thu Dec 17 14:00:20 2009 From: silvinamolinuevo <@t> yahoo.com.ar (Silvina Molinuevo) Date: Thu Dec 17 14:00:23 2009 Subject: [Histonet] Re: veterinary lab question - chem expiration Message-ID: <246186.3984.qm@web113601.mail.gq1.yahoo.com> Hi Jan! with the lote number you can write to the manufacturer and they will inform you expiration times. Best wishes, sil Yahoo! Cocina Encontra las mejores recetas con Yahoo! Cocina. http://ar.mujer.yahoo.com/cocina/ From rjbuesa <@t> yahoo.com Thu Dec 17 14:39:15 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 17 14:39:20 2009 Subject: [Histonet] Staffing for IHC In-Reply-To: <4B2A2736.2B7F.00C9.0@geisinger.edu> Message-ID: <262185.83816.qm@web65712.mail.ac4.yahoo.com> The range for histotechs "doing other tasks" is from 200 to 78,300 slides/year (1 to 301 slides/day). The threshold from 99 laboratories is 9,500 slides/year = 37 slides/day per histotech. Ren? J. --- On Thu, 12/17/09, Angela Bitting wrote: From: Angela Bitting Subject: [Histonet] Staffing for IHC To: histonet@lists.utsouthwestern.edu Date: Thursday, December 17, 2009, 12:42 PM I'm hoping some of the larger hospitals out there will share their data with me. I'm curious as to how labs are staffed for doing IF/IHC/CISH. A small portion of our staining protocols still require pretreatment by hand, but we run instruments that do the pretreatments on board for the majority of our stains. We have 2 IHC techs, 1 on 1st shift and 1 on 3rd shift.Together they are cutting and staining between 200-350 slides per day. I think this is a high volume for only two people. What do you all think? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NKlemme <@t> sakuraus.com Thu Dec 17 14:58:53 2009 From: NKlemme <@t> sakuraus.com (Nancy Klemme) Date: Thu Dec 17 15:00:24 2009 Subject: [Histonet] Silver Nitrate instead of inking?? In-Reply-To: References: Message-ID: <782E3A02C2EB2347BEA6DEA69DC7AB8669D9A01A6A@sfamail.SAKURAUS.LOCAL> Dear Sheila, I would strongly recommend that you obtain approval from the manufacturer of the tissue processors into which you will be putting these tissues before you would begin this practice. The negative accumulative affect on several of the internal components of the instrument can (and probably will) eventually cause the instrument to fail. You would see the effect of the silver nitrate on any metal products it would come into contact with: forceps, metal holding container for the racks or baskets, the racks or baskets themselves if they are metal. It is not approved for use on any model of Tissue Tek Tissue Processors. Many years ago this practice was tolerated by the old tissue-transfer processors. Some people even used this inking method and placed tissues on earlier models of the fluid-transfer processors. Service that was required in those years did not include investigation as to what was placed into the instrument (not simply the reagents, but what also came in on the specimens) that could have been the root cause of the problem. I hope this informative and that you continue to use your current inking product or investigate others that are safer for you and your processors. With kind regards and wonderful holiday wishes, Nancy Klemme EduSvcsDir - Sakura Finetek -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Thursday, December 17, 2009 3:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silver Nitrate instead of inking?? Hi Everyone, Has anyone ever heard of using Silver Nitrate for inking skin bx's before processing??? One of our paths mentioned it. Thanks in advance. Sheila Adey HT MLT _________________________________________________________________ Eligible CDN College & University students can upgrade to Windows 7 before Jan 3 for only $39.99. Upgrade now! http://go.microsoft.com/?linkid=9691819_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From srodriguez <@t> phenopath.com Thu Dec 17 15:36:43 2009 From: srodriguez <@t> phenopath.com (Stephanie Rodriguez) Date: Thu Dec 17 15:36:55 2009 Subject: [Histonet] Re: Histonet Digest, Vol 73, Issue 26 Message-ID: Hi Linda, We routinely run this antibody, and would be happy to perform it for you. Feel free to contact our Client Services department at the phone number below regarding specimen submission. Stephanie Rodriguez, HTL(ASCP), QIHC IHC Tech III Phenopath Laboratories 551 N. 34th St Ste 100 Seattle, WA 98103 (206) 374-9000 > > Message: 8 > Date: Thu, 17 Dec 2009 09:13:40 -0600 > From: "Sebree Linda A" > Subject: [Histonet] hsNFS(INI1) antibody > To: "Histonet" > Message-ID: > <8C023B4AB999614BA4791BAEB26E27381BF6E7@UWHC-MAIL01.uwhis.hosp.wisc.edu> > > Content-Type: text/plain; charset="us-ascii" > > Hi, > > I'm looking for a reference lab that performs an IHC stain for this > antigen. Its used for diagnosing malignant rhabdoid tumor where this > antigen is lost. It is expressed in normal tissue. > > Thanks, > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From tjasper <@t> copc.net Thu Dec 17 16:53:00 2009 From: tjasper <@t> copc.net (Thomas Jasper) Date: Thu Dec 17 16:53:06 2009 Subject: [Histonet] Staffing for IHC References: <4B2A2736.2B7F.00C9.0@geisinger.edu> Message-ID: <90354A475B420441B2A0396E5008D496731A5E@copc-sbs.COPC.local> I would totally agree Angela. Without knowing all the details, I'd bet these people are plenty busy all day everyday. I'd worry about burning them out. And what happens when one or the other is sick or needs a vacation? Tom J Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, December 17, 2009 9:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staffing for IHC I'm hoping some of the larger hospitals out there will share their data with me. I'm curious as to how labs are staffed for doing IF/IHC/CISH. A small portion of our staining protocols still require pretreatment by hand, but we run instruments that do the pretreatments on board for the majority of our stains. We have 2 IHC techs, 1 on 1st shift and 1 on 3rd shift.Together they are cutting and staining between 200-350 slides per day. I think this is a high volume for only two people. What do you all think? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pat.Bell <@t> ucdenver.edu Thu Dec 17 17:00:03 2009 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Thu Dec 17 17:00:10 2009 Subject: [Histonet] Vimentin for Mouse Message-ID: <64DB27005E2FD3439E88502D7A5C91218E098F6CEA@CORTEZ.ucdenver.pvt> Thank you all very much for your help regarding the COX2 for the mouse. Now I would like to ask your help regarding Vimentin for FFPE mouse tissue. I have tried Dako and Sigma with no success. I am also not sure of what clone to use. Thanks again. Pat Pat Bell HT(ASCP) Medical Oncology; MS 8117 12801 E 17th Ave. Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu From cforster <@t> umn.edu Thu Dec 17 17:55:45 2009 From: cforster <@t> umn.edu (Colleen Forster) Date: Thu Dec 17 17:55:44 2009 Subject: [Histonet] To my fellow histotechs.... Message-ID: <4B2AC501.5010104@umn.edu> I am gearing up for a new project and have a list of primaries. I would appreciate any help on what others are using for these. The work will be done on human samples. * Cyclin D1 * Cyclin D2 * Cyclin D3 * CD38 * CD56 * CD138 * Kappa * Lambda * BLIMP-1 * XBP-1 * MUM-1 Thanks in advance... Colleen Forster HT(ASCP)QIHC Bionet Histology Service U of MN 612-626-1930 From W.E.J.Hoekert <@t> olvg.nl Fri Dec 18 03:49:09 2009 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Fri Dec 18 03:50:17 2009 Subject: [Histonet] To my fellow histotechs.... References: <4B2AC501.5010104@umn.edu> Message-ID: <1190CB05C44B13409483514729C2FC360C0A65@PAIT42.olvg.nl> * Cyclin D1 : NeoMarkers, SP4 * CD56 : NeoMarkers, 123C3.D5 * CD138 : Dako, M115 * Kappa : Dako, A0191 (Polyclonal) * Lambda : Dako, A0193 (Polyclonal) * MUM-1 : Dako, MUM1p Willem Hoekert Pathology, OLVG The Netherlands Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From vishal7181 <@t> rediffmail.com Fri Dec 18 04:58:13 2009 From: vishal7181 <@t> rediffmail.com (vishal prasad) Date: Fri Dec 18 06:02:05 2009 Subject: [Histonet] manual of Nikon DIAPHOT-TMD inverted fluorescence microscope Message-ID: <20091218105813.5458.qmail@f4mail-235-244.rediffmail.com> hi all if anyone can please provide me the manual of nikon DIAPHOT-TMD inverted fluorescence micrscope.A pdf file will be very handy for me. i will be very thankful warm regards vishal prasad From portera <@t> msu.edu Fri Dec 18 07:23:00 2009 From: portera <@t> msu.edu (Amy Porter) Date: Fri Dec 18 07:23:02 2009 Subject: [Histonet] Vimentin for Mouse References: <64DB27005E2FD3439E88502D7A5C91218E098F6CEA@CORTEZ.ucdenver.pvt> Message-ID: <61D666DE5183403FBAECCEC5DBA369B2@histolab> I believe that Abcam makes a Rabbit anti-Vimentin, we have used in mouse with heat retrieval at ph6 and it worked really well. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Bell, Pat" To: Sent: Thursday, December 17, 2009 6:00 PM Subject: [Histonet] Vimentin for Mouse Thank you all very much for your help regarding the COX2 for the mouse. Now I would like to ask your help regarding Vimentin for FFPE mouse tissue. I have tried Dako and Sigma with no success. I am also not sure of what clone to use. Thanks again. Pat Pat Bell HT(ASCP) Medical Oncology; MS 8117 12801 E 17th Ave. Aurora, Co. 80045 303-724-6077 pat.bell@ucdenver.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alyssa <@t> alliedsearchpartners.com Fri Dec 18 07:53:01 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Dec 18 07:53:07 2009 Subject: [Histonet] NSH Message-ID: Is anyone else having trouble with getting the NSH website online? -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From Timothy.Morken <@t> ucsfmedctr.org Fri Dec 18 10:35:32 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Dec 18 10:35:59 2009 Subject: [Histonet] NSH webiste problems? In-Reply-To: References: Message-ID: <1AAF670737F193429070841C6B2ADD4C01209818C8@EXMBMCB15.ucsfmedicalcenter.org> Yes, the website seems to be down. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alyssa Peterson Sent: Friday, December 18, 2009 5:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Is anyone else having trouble with getting the NSH website online? -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From igor.deyneko <@t> gmail.com Fri Dec 18 10:44:09 2009 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Fri Dec 18 10:44:12 2009 Subject: [Histonet] Slide Labelers Message-ID: <35e16a770912180844h55ef409ehc72904d2cd0148a5@mail.gmail.com> Dear Histonetters! I was wondering if anyone out there is using a slide labeler. I would appreciate any info on models(serial numbers), and vendors. We have high throughput and need a more automated system with minimal time to input data. Thank you for any suggestions. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA From micropathlabs <@t> yahoo.com Fri Dec 18 11:03:22 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Fri Dec 18 11:03:25 2009 Subject: [Histonet] VIP Schedules Message-ID: <217013.23982.qm@web57804.mail.re3.yahoo.com> Would anyone be willing to share a "shortened" processing schedule?to use with the VIP? I have one but would like?to compare with what others are doing. I'm also looking for xylene free alternatives?for the VIP and schedules for that type of processing. Any assistance or reference would be appreciated. Thanks in advance! Sheila ? Sheila Haas Laboratory Supervisor Micro Path Laboratories From shive003 <@t> umn.edu Fri Dec 18 11:05:15 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Dec 18 11:05:20 2009 Subject: [Histonet] Slide Labelers References: <35e16a770912180844h55ef409ehc72904d2cd0148a5@mail.gmail.com> Message-ID: <0BFBA07A3BE9499BB5B2A8C9748F47A0@auxs.umn.edu> Leica IP S Slide Printer ----- Original Message ----- From: "Igor Deyneko" To: Sent: Friday, December 18, 2009 10:44 AM Subject: [Histonet] Slide Labelers > Dear Histonetters! > I was wondering if anyone out there is using a slide labeler. I would > appreciate any info on models(serial numbers), and vendors. We have high > throughput and need a more automated system with minimal time to input > data. > > Thank you for any suggestions. > Igor Deyneko > Infinity Pharmaceuticals > Cambridge, MA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Fri Dec 18 11:30:01 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 18 11:30:05 2009 Subject: [Histonet] VIP Schedules In-Reply-To: <217013.23982.qm@web57804.mail.re3.yahoo.com> Message-ID: <377173.25126.qm@web65715.mail.ac4.yahoo.com> Under separate cover I am sending the information you need (schedules and xylene substitute). ren? J. --- On Fri, 12/18/09, Sheila Haas wrote: From: Sheila Haas Subject: [Histonet] VIP Schedules To: histonet@lists.utsouthwestern.edu Date: Friday, December 18, 2009, 12:03 PM Would anyone be willing to share a "shortened" processing schedule?to use with the VIP? I have one but would like?to compare with what others are doing. I'm also looking for xylene free alternatives?for the VIP and schedules for that type of processing. Any assistance or reference would be appreciated. Thanks in advance! Sheila ? Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie <@t> conxis.com Fri Dec 18 12:00:31 2009 From: laurie <@t> conxis.com (laurie@conxis.com) Date: Fri Dec 18 12:00:42 2009 Subject: [Histonet] IGF1R beta recommendations anyone? Message-ID: Happy Holidays everyone, I am looking for an IGF1R beta antibody that works well in human liver and pancreatic tissue. I have one here that I have tried and it worked well on the pancreas and nothing for the liver. Suggestions? Happy Friday! Laurie Laurie Popp, HT(ASCP) From laurie <@t> conxis.com Fri Dec 18 12:02:26 2009 From: laurie <@t> conxis.com (laurie@conxis.com) Date: Fri Dec 18 12:02:35 2009 Subject: [Histonet] ASCP website Message-ID: <0458D41082B944FE814B201B00689FDF.MAI@accuwebhosting.biz> Has anyone had problems on ASCP's website in the last couple of days? I have been trying to enter stuff in on re.member and it has been timing out or not allowing logging in at all...havent' had any problems on NSH's website TGIF! Laurie From rsrichmond <@t> gmail.com Fri Dec 18 13:59:48 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Dec 18 14:00:06 2009 Subject: [Histonet] Re: Silver Nitrate instead of inking?? Message-ID: I've worked in about 60 pathology operations since 1964. I have NEVER seen silver nitrate used for tissue marking, though I've heard of it. I think the point about the silver fouling processors is well taken. In addition, silver has the nasty habit of forming explosive complexes - I don't want to think about it reacting with Bouin's fixative to form silver picrate. There are basically three options. India ink - $4 a bottle from your local crafts store. Special marking inks, of which the Davidson marking inks are perhaps the most widely used, and in my opinion the best (I have no commercial connection with Mrs. Stewart, much less with Martha Stewart). The third option is tattoo inks - cheap, available in an unlimited variety of colors, and in my limited experience with them they get the job done. Bob Richmond Samurai Pathologist Knoxville TN From mbrooks <@t> incytepathology.com Fri Dec 18 14:19:34 2009 From: mbrooks <@t> incytepathology.com (Matt Brooks) Date: Fri Dec 18 14:19:39 2009 Subject: [Histonet] Effects of Inking on Special stains or IHC Message-ID: <706224670091FE47997AEF88EFADE7CA01300AC0@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Hello All, One of my pathologists asked the following question. "Does inking tissue effect special staining or IHC results?" I replied that I had never heard of this and not to my knowledge. I thought that if the chromagen was the same color of the dye then there could be some difficulty in deciphering between the two. Therefore, I am wondering if anyone has experienced a problem. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbrooks@incytepathology.com 509-892-2744 From LRaff <@t> uropartners.com Fri Dec 18 14:22:20 2009 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Fri Dec 18 14:22:25 2009 Subject: FW: [Histonet] Effects of Inking on Special stains or IHC Message-ID: We use six different colors of marking inks (black, blue, purple, yellow, green, orange)and have not noticed any interference on IHC. We did decide not to use red ink since we were concerned it might make the Vulcan fast red chromogen used in our PIN4 cocktail hard to interpret. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Brooks Sent: Friday, December 18, 2009 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Effects of Inking on Special stains or IHC Hello All, One of my pathologists asked the following question. "Does inking tissue effect special staining or IHC results?" I replied that I had never heard of this and not to my knowledge. I thought that if the chromagen was the same color of the dye then there could be some difficulty in deciphering between the two. Therefore, I am wondering if anyone has experienced a problem. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbrooks@incytepathology.com 509-892-2744 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ Information from ESET NOD32 Antivirus, version of virus signature database 4700 (20091218) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com __________ Information from ESET NOD32 Antivirus, version of virus signature database 4700 (20091218) __________ The message was checked by ESET NOD32 Antivirus. http://www.eset.com From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Dec 18 14:50:34 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Dec 18 14:51:26 2009 Subject: [Histonet] Re: Silver Nitrate instead of inking?? In-Reply-To: References: Message-ID: We in our lab use regular household white vinegar to help the ink stay on the tissue during processing, cheap and safe. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond [rsrichmond@gmail.com] Sent: Friday, December 18, 2009 2:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Silver Nitrate instead of inking?? I've worked in about 60 pathology operations since 1964. I have NEVER seen silver nitrate used for tissue marking, though I've heard of it. I think the point about the silver fouling processors is well taken. In addition, silver has the nasty habit of forming explosive complexes - I don't want to think about it reacting with Bouin's fixative to form silver picrate. There are basically three options. India ink - $4 a bottle from your local crafts store. Special marking inks, of which the Davidson marking inks are perhaps the most widely used, and in my opinion the best (I have no commercial connection with Mrs. Stewart, much less with Martha Stewart). The third option is tattoo inks - cheap, available in an unlimited variety of colors, and in my limited experience with them they get the job done. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Dec 18 15:21:09 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 18 15:21:13 2009 Subject: [Histonet] Effects of Inking on Special stains or IHC In-Reply-To: <706224670091FE47997AEF88EFADE7CA01300AC0@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Message-ID: <861348.79623.qm@web65701.mail.ac4.yahoo.com> Your answer was the correct answer. Ren? J. --- On Fri, 12/18/09, Matt Brooks wrote: From: Matt Brooks Subject: [Histonet] Effects of Inking on Special stains or IHC To: histonet@lists.utsouthwestern.edu Date: Friday, December 18, 2009, 3:19 PM Hello All, One of my pathologists asked the following question.? "Does inking tissue effect special staining or IHC results?"? I replied that I had never heard of this and not to my knowledge.? I thought that if the chromagen was the same color of the dye then there could be some difficulty in deciphering between the two.? Therefore, I am wondering if anyone has experienced a problem. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbrooks@incytepathology.com 509-892-2744 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Fri Dec 18 15:24:04 2009 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Dec 18 15:24:14 2009 Subject: [Histonet] Re: Silver Nitrate instead of inking?? In-Reply-To: References: Message-ID: <8FE906B59F05419CB673544C83BCC4F4@BryanPC> I did work in a lab that used silver nitrate to mark cervical biopsies. Most of the pathologists used a 1% solution, often dipping the whole cervix into it and causing the whole lot to precipitate from the formalin (grrr). One used four or five silver nitrate sticks by dipping in water and just rubbing over the specimen. It does work, a black porecipitate coating the edge. I have never heard of any safety concerns with it as the specimen was placed back into formalin immediately after the coating for processing and reduction seems to be total. What I did note was that the silver nitrate sometimes penetrated the tissue and you could see s rim of fine granules about a cell deep into the surface which could be distracting. I then got them to switch to India ink, but I found that messy, so I switched again to Blueing, which was relatively clean and convenient. I also obtained some tattoo inks so different surfaces could be marked if necessary From DKnutson <@t> primecare.org Fri Dec 18 15:42:01 2009 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Fri Dec 18 15:42:56 2009 Subject: [Histonet] (no subject) Message-ID: <4F0B7161A6CD524FAD8017D52E1553400D2B6967@exchangent> Histonetters, Does anyone out there in histoland have a job description in their lab for staff that are like aides or assistants? They would be entering specimens, distributing slides, logging in placentas, tossing specimens, and just basically assisting the technical staff in any way possible. It would not require a college degree. I was wondering what you might call that job description? And would it be possible for you to share your explanation and requirements for a job similar to the one I described? Thank you ahead of time for any examples you have. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org From rsrichmond <@t> gmail.com Fri Dec 18 15:43:22 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Dec 18 15:43:27 2009 Subject: [Histonet] Re: Silver Nitrate instead of inking?? In-Reply-To: References: Message-ID: Loralee McMahon, HTL (ASCP), Immunohistochemistry Supervisor, Department of Surgical Pathology, Strong Memorial Hospital, Rochester NY notes: >>We in our lab use regular household white vinegar to help the ink stay on the tissue during processing, cheap and safe.<< I forgot to mention this, because I personally don't use it - I find that if you take the time to blot an unfixed or fixed specimen thoroughly dry before inking, the ink stays on quite satisfactorily. But if the pathologist does want to use something, then 3% acetic acid (which can be ordinary household white vinegar diluted 1 to 1 with water) should be offered. Don't use Bouin's fixative (we all know the varied problems presented by picric acid), nor acetone (flammable). Bob Richmond Samurai Pathologist Knoxville TN From Laura.Miller <@t> leica-microsystems.com Fri Dec 18 16:02:53 2009 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Fri Dec 18 16:03:00 2009 Subject: [Histonet] Laura Miller is Out of the Office. Message-ID: I will be out of the office starting 12/18/2009 and will not return until 12/29/2009. I will repsond to your message when I return on December 29th! HAPPY HOLIDAYS! ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From ndevans <@t> stanford.edu Fri Dec 18 18:12:10 2009 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Fri Dec 18 18:12:15 2009 Subject: [histonet] Muscle Message-ID: <1A2405A34D0546A3B398FF4779ABA765@DellDesktop2> Dear All, I was wondering whether anyone had a good recommendation for a textbook on the physiology and biochemistry of muscle? Also I was wondering whether anyone could recommend a good antibody for myosin heavy chain, fast type (type I)? Best wishes Nick From ccrowder <@t> vetmed.lsu.edu Fri Dec 18 18:18:26 2009 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Fri Dec 18 18:21:12 2009 Subject: [Histonet] Slide labeler Message-ID: Igor - We have a Leica slide labeler. It is easy to use, but we have found that you should use slides with 90 degree corners and a "rough" surface. Slides with clipped corners tend to slide through the chute and get caught inside the machine. It's easy to extract them but it's a hassle. Also the ink and light bulb are expensive, but we wouldn't give ours up for anything. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From cforster <@t> umn.edu Fri Dec 18 18:41:37 2009 From: cforster <@t> umn.edu (Colleen Forster) Date: Fri Dec 18 18:41:43 2009 Subject: [Histonet] Slide labeler In-Reply-To: References: Message-ID: <4B2C2141.1080405@umn.edu> And if you have their coverslipper, it doesn't like the rounded edges either!! Colleen Forster. Cheryl Crowder wrote: > Igor - We have a Leica slide labeler. It is easy to use, but we have found > that you should use slides with 90 degree corners and a "rough" surface. > Slides with clipped corners tend to slide through the chute and get caught > inside the machine. It's easy to extract them but it's a hassle. Also > the ink and light bulb are expensive, but we wouldn't give ours up for > anything. > Cheryl > > Cheryl Crowder, BA, HTL(ASCP) > Chief Technologist > Anatomic Pathology > Department of Pathobiological Sciences > School of Veterinary Medicine > Louisiana State University > Skip Bertman Drive > Baton Rouge, LA 70803 > > 225-578-9734 > FAX: 225-578-9720 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Maxim_71 <@t> mail.ru Sun Dec 20 11:18:36 2009 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sun Dec 20 11:18:42 2009 Subject: [Histonet] Myocardial biopsies Message-ID: <20331308.20091220201836@mail.ru> Dear colleagues! My pathologists asked me about books for myocardial biopsies (Histopathology of Myocard; Endomyocardial Biopsy). Can you recommend any good books for pathologists and techs about these issue, please? We are new on this field. Thanks you for advise. Sincerely, Maxim Peshkov, Taganrog, Russia. mailto:Maxim_71@mail.ru From trathborne <@t> somerset-healthcare.com Mon Dec 21 07:53:02 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Dec 21 07:53:08 2009 Subject: [Histonet] Slide labeler In-Reply-To: <4B2C2141.1080405@umn.edu> Message-ID: We have the coverslipper and have not had any problems with the clipped-corner slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Colleen Forster Sent: Friday, December 18, 2009 7:42 PM To: Cheryl Crowder Cc: Histonet Subject: Re: [Histonet] Slide labeler And if you have their coverslipper, it doesn't like the rounded edges either!! Colleen Forster. Cheryl Crowder wrote: > Igor - We have a Leica slide labeler. It is easy to use, but we have found > that you should use slides with 90 degree corners and a "rough" surface. > Slides with clipped corners tend to slide through the chute and get caught > inside the machine. It's easy to extract them but it's a hassle. Also > the ink and light bulb are expensive, but we wouldn't give ours up for > anything. > Cheryl > > Cheryl Crowder, BA, HTL(ASCP) > Chief Technologist > Anatomic Pathology > Department of Pathobiological Sciences > School of Veterinary Medicine > Louisiana State University > Skip Bertman Drive > Baton Rouge, LA 70803 > > 225-578-9734 > FAX: 225-578-9720 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From jshelley <@t> burnham.org Mon Dec 21 08:38:55 2009 From: jshelley <@t> burnham.org (John Shelley) Date: Mon Dec 21 08:39:00 2009 Subject: [Histonet] Integrin Alpha V on mouse tumor tissue Message-ID: Hi Histonetters, I have been requested to run Integrin Alpha V and Hif-1 Alpha on tumor tissue of a mouse and looking for anyone who has run these antibodies and what antibody they use with the protocol run. I have tried both with no success and even had application techs from IHC companies try to help with no avail. Any recommendations would be appreciated. John May you find this Christmas season the hope, peace and joy that comes from the birth of the babe! From Lizbeth_Kelly <@t> hgsi.com Mon Dec 21 09:28:16 2009 From: Lizbeth_Kelly <@t> hgsi.com (Lizbeth Kelly) Date: Mon Dec 21 09:32:52 2009 Subject: [Histonet] Re: Leica slide printer Message-ID: <58E71CC87E47A24EAE1CAABC8ADC202A049C9C0032@EXMBX1.corp.hgsi.com> Hi, We are in the process of purchasing a slide printer. Any good recommendation in using a Leica slide printer? If you are using a slide printer you like and would like to share the advantages from the Leica, any information given will be appreciated. Thanks, Lizbeth Kelly Human Genome Sciences From steve <@t> myelinrepair.org Mon Dec 21 10:03:21 2009 From: steve <@t> myelinrepair.org (Steve Wong) Date: Mon Dec 21 10:03:25 2009 Subject: [Histonet] Looking for a CRO recommendation Message-ID: Hello, I am looking for a recommendation for a CRO that can perform simple volumetric calculations from serial sections of a lesion in the spinal cord. Closer to the SF Bay area is better, but not required. Thanks! Steve From Maria.Katleba <@t> stjoe.org Mon Dec 21 10:12:03 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Mon Dec 21 10:12:26 2009 Subject: [Histonet] URGENT! question about Autopsy Hours Message-ID: I have to have the following answers ASAP.... Can anyone tell me how much work (hours of service provided) is associated with each of the following : (1) Autopsy (paid at $1200-1300 each) (2) Autopsy for infants with SIDS ($2000- 2500each) (3) Diener services (at $150-250 each) Thanks in advance!!! Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From GaleL <@t> unionhospital.org Mon Dec 21 12:24:06 2009 From: GaleL <@t> unionhospital.org (Gale Limron) Date: Mon Dec 21 12:24:25 2009 Subject: [Histonet] Leica ASP300S processor Message-ID: We are a smaller hospital and use one processing run each night on a newer Leica ASP300S for all specimens. Though we usually allow "big" specimens extra fixation time we still have many larger specimens that are not completely fixed after processing. Does anyone have a processing protocol that might help us without having to process large and small specimens separately? Gale Limron GALEL@unionhospital.org Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext. 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From liz <@t> premierlab.com Mon Dec 21 12:31:07 2009 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Dec 21 12:31:11 2009 Subject: [Histonet] Leica ASP300S processor In-Reply-To: Message-ID: What did you do with your older stainer? You could use that for biopsies. When I was in clinical we just used an old dip and dunk Shandon for our biopsies, we took it out of storage. We never had to deal with overprocessed biopsies again and we could run the larger tissue samples on the other tissue processor. The other thing is how thick do your pathologist gross the tissue in? If they are cutting tissue that is too thick you are just going to run into problems. Just some suggestions Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Monday, December 21, 2009 11:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica ASP300S processor We are a smaller hospital and use one processing run each night on a newer Leica ASP300S for all specimens. Though we usually allow "big" specimens extra fixation time we still have many larger specimens that are not completely fixed after processing. Does anyone have a processing protocol that might help us without having to process large and small specimens separately? Gale Limron GALEL@unionhospital.org Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext. 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Mon Dec 21 13:19:01 2009 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Mon Dec 21 13:19:22 2009 Subject: [Histonet] Seasons Greetings/Thank you Dr Margraf. Message-ID: <8CC5089962B237F-3D18-332DE@webmail-d026.sysops.aol.com> Seasons Greetings to everyone from the Heart of Dixie, and thank you Dr Linda Margraf and colleagues for hosting the "Histonet". I get to keep in touch and not have to leave my office! Michael Titford USA Pathology Mobile AL USA From aevans <@t> wellspan.org Mon Dec 21 13:19:36 2009 From: aevans <@t> wellspan.org (Evans, Andria B) Date: Mon Dec 21 13:19:43 2009 Subject: [Histonet] Recycling Formalin, Xylene, and Alcohol Message-ID: I would like to know what everyone out in histoland is using to recycle there solutions. We are looking at switching out the procyclers (that are about 10 years old) to something else. Any Pros/Cons of current methods would be great. Thanks!! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. . ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From sklan <@t> illinois.edu Mon Dec 21 13:25:08 2009 From: sklan <@t> illinois.edu (sklan@illinois.edu) Date: Mon Dec 21 13:25:12 2009 Subject: [Histonet] Sanderson's rapid bone stain + Acid fuchsin Message-ID: <20091221132508.CIT26219@expms5.cites.uiuc.edu> Hi All - I am trying to figure out if the combination of Sanderson's rapid bone stain and acid fuchsin as the counterstain (or methylene blue + basic fuchsin) results in pink/red staining of biologically formed apatite (apatite not associated with collagen produced by osteoblasts)? An associated question is if the dye-tissue interaction between acid or basic fuchsin and mineralized bone is due to dye-collagen interactions? If anyone has any experiences or references that may help, please let me know. Thank you for your help, Sheeny From Lizbeth_Kelly <@t> hgsi.com Mon Dec 21 13:52:12 2009 From: Lizbeth_Kelly <@t> hgsi.com (Lizbeth Kelly) Date: Mon Dec 21 13:52:26 2009 Subject: [Histonet] Re: slide printers Message-ID: <58E71CC87E47A24EAE1CAABC8ADC202A049C9C0039@EXMBX1.corp.hgsi.com> Hi, Can someone in histoland share names of slide printers that are satisfied with? I prefer no etchers. Thanks, Lizbeth Kelly Human Genome Sciences Rockville, Maryland From laurie.colbert <@t> huntingtonhospital.com Mon Dec 21 14:51:30 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Dec 21 14:51:36 2009 Subject: [Histonet] Re: slide printers Message-ID: <57BE698966D5C54EAE8612E8941D7683078F4A43@EXCHANGE3.huntingtonhospital.com> We have 5 of the Fisher Slidemates, and I love them. We've had some issues with them, but I still recommend them. The units use a ribbon to print the information. Our cassettes are barcoded, and the Slidemate reads the barcode on the cassette and prints up the corresponding slide. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lizbeth Kelly Sent: Monday, December 21, 2009 11:52 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: slide printers Hi, Can someone in histoland share names of slide printers that are satisfied with? I prefer no etchers. Thanks, Lizbeth Kelly Human Genome Sciences Rockville, Maryland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dont8know8me <@t> yahoo.com Mon Dec 21 15:07:20 2009 From: dont8know8me <@t> yahoo.com (richard peralta) Date: Mon Dec 21 15:07:25 2009 Subject: [Histonet] mucle biopsy Message-ID: <230554.1522.qm@web51404.mail.re2.yahoo.com> hello histoneters ? I need information on turn around time on muscle biopsy for immunoflour.....light microscope etc..... ? ty in advance for ur input ? HAPPY HOLIDAYS From JWeems <@t> sjha.org Mon Dec 21 15:08:00 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Dec 21 15:08:00 2009 Subject: [Histonet] Seasons Greetings/Thank you Dr Margraf. In-Reply-To: <8CC5089962B237F-3D18-332DE@webmail-d026.sysops.aol.com> References: <8CC5089962B237F-3D18-332DE@webmail-d026.sysops.aol.com> Message-ID: Here here!!! Merry Christmas, my Histo Family. Have a safe holiday. And thanks to Herb and Dr. Margraf 70 x 70 trillion... J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Monday, December 21, 2009 14:19 To: Histonet@pathology.swmed.edu Subject: [Histonet] Seasons Greetings/Thank you Dr Margraf. Seasons Greetings to everyone from the Heart of Dixie, and thank you Dr Linda Margraf and colleagues for hosting the "Histonet". I get to keep in touch and not have to leave my office! Michael Titford USA Pathology Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From ratliffjack <@t> hotmail.com Mon Dec 21 15:30:25 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon Dec 21 15:30:41 2009 Subject: [Histonet] Sanderson's rapid bone stain + Acid fuchsin In-Reply-To: <20091221132508.CIT26219@expms5.cites.uiuc.edu> References: <20091221132508.CIT26219@expms5.cites.uiuc.edu> Message-ID: Regarding the counterstain for use with Sanderson's Rapid Bone Stain, I use the Van Gieson picrofuchsin in that it will stain older, mature, mineralized bone red. As an additional consequence of the VG counterstain, the originally blue stained dense collagen (new bone/ osteoid) from the SRBS will change to a greenish color from the reaction of the yellow picric acid (present in the counterstain). If you think about it, it makes perfect sense due to the basic reaction of combining blue and yellow to yield green. Additionally, the intensity of the red from the acid fuchsin content of the counterstain is directly proportional to the mineral density of the bone due to the acid in the solution acting to lightly etch the mineralized bone. Also, think of the specimen in 3D, you could expect shades of red depending on the plane of sectioning within the specimen and depending upon mineral deposition at the surface of trabeculae. Therefore, I believe that the dye-tissue interaction you are inquiring about is associated to mineralization or calcium concentration. Hope this helps to answer your question. Jack On Dec 21, 2009, at 1:25 PM, wrote: > Hi All - > > I am trying to figure out if the combination of Sanderson's rapid > bone stain and acid fuchsin as the counterstain (or methylene blue + > basic fuchsin) results in pink/red staining of biologically formed > apatite (apatite not associated with collagen produced by > osteoblasts)? An associated question is if the dye-tissue > interaction between acid or basic fuchsin and mineralized bone is > due to dye-collagen interactions? > > If anyone has any experiences or references that may help, please > let me know. > > Thank you for your help, > > Sheeny > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tkngflght <@t> yahoo.com Mon Dec 21 18:11:01 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Dec 21 18:11:05 2009 Subject: [Histonet] IHC QC slide management Message-ID: <643069.86098.qm@web50901.mail.re2.yahoo.com> ?Hi Guys- ? You're all batting 500 so here's another query: ? We run a lot of Immunos, not a huge variety but we need to manage how we track patients that are positive and can be used for controls when needed. ? Does anyone have a simple spreadsheet or concept I could put into a document to make this easy for the people who rotate through this bench to manage well? ? LOVE labs at Christmas--we're running on chocolate!!! ? Cheryl Kerry, HT(ASCP) From wdesalvo.cac <@t> hotmail.com Mon Dec 21 19:04:20 2009 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Mon Dec 21 19:04:25 2009 Subject: [Histonet] IHC QC slide management In-Reply-To: <643069.86098.qm@web50901.mail.re2.yahoo.com> References: <643069.86098.qm@web50901.mail.re2.yahoo.com> Message-ID: Depending on the LIS you have, you may be able to have the pathologist "flag" the case/cases as they read and then you can pull a report. This works for the two systems we use, Cerner MLLE and Novovision. William DeSalvo, B.S., HTL(ASCP) > Date: Mon, 21 Dec 2009 16:11:01 -0800 > From: tkngflght@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC QC slide management > > > > > Hi Guys- > > You're all batting 500 so here's another query: > > We run a lot of Immunos, not a huge variety but we need to manage how we track patients that are positive and can be used for controls when needed. > > Does anyone have a simple spreadsheet or concept I could put into a document to make this easy for the people who rotate through this bench to manage well? > > LOVE labs at Christmas--we're running on chocolate!!! > > Cheryl Kerry, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/177141665/direct/01/ From rjcarp <@t> usiwireless.com Mon Dec 21 23:51:09 2009 From: rjcarp <@t> usiwireless.com (Randall Carpenter) Date: Mon Dec 21 23:51:14 2009 Subject: [Histonet] Trichrome stain on MMA sections Message-ID: Dear Histonet, I was wondering what the best Trichrome stain might be for sawn and ground sections of large stent/artery in methylmethacrylate. I am also looking to stain elastic fibers. Any suggestions? Thanks. Randy Carpenter From mburton1 <@t> bu.edu Tue Dec 22 07:42:29 2009 From: mburton1 <@t> bu.edu (mburton1@bu.edu) Date: Tue Dec 22 07:43:40 2009 Subject: [Histonet] Re: slide printers In-Reply-To: <58E71CC87E47A24EAE1CAABC8ADC202A049C9C0039@EXMBX1.corp.hgsi.com> References: <58E71CC87E47A24EAE1CAABC8ADC202A049C9C0039@EXMBX1.corp.hgsi.com> Message-ID: <20091222084229.z2avdjsw2swokscw@www.bu.edu> We are currently demoing the Fisher Slidemate slide printer and so far we love it. Works well at individual stations but probably not for large batch printing. Mark A Burton HTL ASCP Mass. General Hospital Boston, MA Quoting Lizbeth Kelly : > Hi, > > Can someone in histoland share names of slide printers that are > satisfied with? I prefer no etchers. > Thanks, > > Lizbeth Kelly > Human Genome Sciences > Rockville, Maryland > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From MSTONE <@t> CMHLINK.ORG Tue Dec 22 08:00:22 2009 From: MSTONE <@t> CMHLINK.ORG (MARCELLYN STONE) Date: Tue Dec 22 08:01:05 2009 Subject: [Histonet] sharpen permanent knife blades Message-ID: <006801ca830f$2dc7fe30$fc050180@corp.cmhlink.org> Please help:-)..... I am looking for information on anyone who sharpens permanent knife blades. Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410) 535-8282 and destroy all copies of this communication and any attachments. From asmith <@t> mail.barry.edu Tue Dec 22 08:07:27 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Dec 22 08:09:04 2009 Subject: [Histonet] sharpen permanent knife blades In-Reply-To: <006801ca830f$2dc7fe30$fc050180@corp.cmhlink.org> References: <006801ca830f$2dc7fe30$fc050180@corp.cmhlink.org> Message-ID: C.L. Sturkey (www.sturkey.com) in Lebanon, Pennsylvania, sharpens permanent microtome knives. They also have two grades of disposable blades. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Podiatric Medicine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARCELLYN STONE Sent: Tuesday, December 22, 2009 9:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sharpen permanent knife blades Please help:-)..... I am looking for information on anyone who sharpens permanent knife blades. Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410) 535-8282 and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Tue Dec 22 09:06:03 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Dec 22 09:06:00 2009 Subject: [Histonet] sharpen permanent knife blades In-Reply-To: References: <006801ca830f$2dc7fe30$fc050180@corp.cmhlink.org> Message-ID: I will also add that DDK and Dorn and Hart Microedge also sharpen knives. One should also check the pricing as they all vary. Jack Sent from my iPhone On Dec 22, 2009, at 8:07 AM, "Smith, Allen" wrote: > C.L. Sturkey (www.sturkey.com) in Lebanon, Pennsylvania, sharpens > permanent microtome knives. They also have two grades of disposable > blades. > > Allen A. Smith, Ph.D. > Professor of Anatomy > Barry University School of Podiatric Medicine > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of MARCELLYN STONE > Sent: Tuesday, December 22, 2009 9:00 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] sharpen permanent knife blades > > Please help:-)..... > > I am looking for information on anyone who sharpens permanent knife > blades. > > Thanks Marcy > > > > > CONFIDENTIALITY NOTICE: > > This e-mail communication and any attachments may contain > confidential and > privileged information for use by the designated recipients named > above. They > are intended solely for these recipients. If you are not the intended > recipient, > you are hereby notified that you have received this communication in > error and > that any review, disclosure, dissemination, distribution or copying > of it or > its > contents is prohibited. If you have received this communication in > error, > please > notify Calvert Memorial Hospital immediately by telephone at (410) 535-8282 > and > destroy all copies of this communication and any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ratliffjack <@t> hotmail.com Tue Dec 22 09:15:03 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Dec 22 09:14:59 2009 Subject: [Histonet] Trichrome stain on MMA sections In-Reply-To: References: Message-ID: You can do H&E and VVG quite nicely on this type of MMA embedded tissue. Maybe you could also try a Sanderson's (methylene blue) with the Van Gieson (acid fuchsin w/ picric acid) counterstain??? On Dec 21, 2009, at 11:51 PM, Randall Carpenter wrote: > Dear Histonet, > > I was wondering what the best Trichrome stain might be for sawn and > ground sections of large stent/artery in methylmethacrylate. I am > also looking to stain elastic fibers. Any suggestions? Thanks. > > Randy Carpenter > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mucram11 <@t> comcast.net Tue Dec 22 09:36:43 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Tue Dec 22 09:36:47 2009 Subject: [Histonet] sharpen permanent knife blades In-Reply-To: Message-ID: <1144603043.122911261496203866.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Different companies have varied angles on how a knife is sharpened for their equipment.? A knife sharpened by one company will generally be somewhat different in?the angle of the edge than another when you begin cutting.? Some may have a flatter edge or a fatter edge at the top cutting portion.? These are not always easy to see and can cause issues with sectioning although sharpness is the same or very? close.? Using several different companies can lead to problems in adjusting the knife angle for sectioning from one to another.? I would select one company and stay with it to avoid these issues.? I found a company I preferred and then stuck with them rather than fight the problems over a few dollars. Pam Marcum ----- Original Message ----- From: "Jack Ratliff" To: "Allen Smith" Cc: Histonet@lists.utsouthwestern.edu, mstone@cmhlink.org Sent: Tuesday, December 22, 2009 9:06:03 AM GMT -06:00 US/Canada Central Subject: Re: [Histonet] sharpen permanent knife blades I will also add that DDK and Dorn and Hart Microedge also sharpen ? knives. One should also check the pricing as they all vary. Jack Sent from my iPhone On Dec 22, 2009, at 8:07 AM, "Smith, Allen" ? wrote: > C.L. Sturkey (www.sturkey.com) in Lebanon, Pennsylvania, sharpens ? > permanent microtome knives. ?They also have two grades of disposable ? > blades. > > Allen A. Smith, Ph.D. > Professor of Anatomy > Barry University School of Podiatric Medicine > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of MARCELLYN STONE > Sent: Tuesday, December 22, 2009 9:00 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] sharpen permanent knife blades > > Please help:-)..... > > I am looking for information on anyone who sharpens permanent knife ? > blades. > > Thanks Marcy > > > > > CONFIDENTIALITY NOTICE: > > This e-mail communication and any attachments may contain ? > confidential and > privileged information for use by the designated recipients named ? > above. They > are intended solely for these recipients. If you are not the intended > recipient, > you are hereby notified that you have received this communication in ? > error and > that any review, disclosure, dissemination, distribution or copying ? > of it or > its > contents is prohibited. If you have received this communication in ? > error, > please > notify Calvert Memorial Hospital immediately by telephone at (410) 535-8282 > and > destroy all copies of this communication and any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Tue Dec 22 10:31:03 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Dec 22 10:31:08 2009 Subject: [Histonet] sharpen permanent knife blades In-Reply-To: <1144603043.122911261496203866.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: , <1144603043.122911261496203866.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: I agree with most of what you have stated and espcially with sticking with one company once you get things moving in the right direction. However, if one truly knows how to properly set and/or adjust cutting angles at the microtome, I would definely keep cost at a high consideration if quality is uniform across vendors. Of course, everyone also has various demands as related to workload volume and study turnaround time. These variables should also be considered and again, especially if product quality is not compromised. Jack Ratliff Date: Tue, 22 Dec 2009 15:36:43 +0000 From: mucram11@comcast.net To: ratliffjack@hotmail.com CC: Histonet@lists.utsouthwestern.edu; mstone@cmhlink.org; asmith@mail.barry.edu Subject: Re: [Histonet] sharpen permanent knife blades Different companies have varied angles on how a knife is sharpened for their equipment. A knife sharpened by one company will generally be somewhat different in the angle of the edge than another when you begin cutting. Some may have a flatter edge or a fatter edge at the top cutting portion. These are not always easy to see and can cause issues with sectioning although sharpness is the same or very close. Using several different companies can lead to problems in adjusting the knife angle for sectioning from one to another. I would select one company and stay with it to avoid these issues. I found a company I preferred and then stuck with them rather than fight the problems over a few dollars. Pam Marcum ----- Original Message ----- From: "Jack Ratliff" To: "Allen Smith" Cc: Histonet@lists.utsouthwestern.edu, mstone@cmhlink.org Sent: Tuesday, December 22, 2009 9:06:03 AM GMT -06:00 US/Canada Central Subject: Re: [Histonet] sharpen permanent knife blades I will also add that DDK and Dorn and Hart Microedge also sharpen knives. One should also check the pricing as they all vary. Jack Sent from my iPhone On Dec 22, 2009, at 8:07 AM, "Smith, Allen" wrote: > C.L. Sturkey (www.sturkey.com) in Lebanon, Pennsylvania, sharpens > permanent microtome knives. They also have two grades of disposable > blades. > > Allen A. Smith, Ph.D. > Professor of Anatomy > Barry University School of Podiatric Medicine > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of MARCELLYN STONE > Sent: Tuesday, December 22, 2009 9:00 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] sharpen permanent knife blades > > Please help:-)..... > > I am looking for information on anyone who sharpens permanent knife > blades. > > Thanks Marcy > > > > > CONFIDENTIALITY NOTICE: > > This e-mail communication and any attachments may contain > confidential and > privileged information for use by the designated recipients named > above. They > are intended solely for these recipients. If you are not the intended > recipient, > you are hereby notified that you have received this communication in > error and > that any review, disclosure, dissemination, distribution or copying > of it or > its > contents is prohibited. If you have received this communication in > error, > please > notify Calvert Memorial Hospital immediately by telephone at (410) 535-8282 > and > destroy all copies of this communication and any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Cline <@t> wchsys.org Tue Dec 22 12:00:16 2009 From: Joyce.Cline <@t> wchsys.org (Joyce Cline) Date: Tue Dec 22 12:03:21 2009 Subject: [Histonet] IHC QC slide management In-Reply-To: <643069.86098.qm@web50901.mail.re2.yahoo.com> References: <643069.86098.qm@web50901.mail.re2.yahoo.com> Message-ID: We have a column on the quality control sheet we give the pathologist with the slides. The pathologist marks the column for + patient slide. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl [tkngflght@yahoo.com] Sent: Monday, December 21, 2009 7:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC QC slide management Hi Guys- You're all batting 500 so here's another query: We run a lot of Immunos, not a huge variety but we need to manage how we track patients that are positive and can be used for controls when needed. Does anyone have a simple spreadsheet or concept I could put into a document to make this easy for the people who rotate through this bench to manage well? LOVE labs at Christmas--we're running on chocolate!!! Cheryl Kerry, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From mjorgensen08 <@t> gmail.com Tue Dec 22 12:32:08 2009 From: mjorgensen08 <@t> gmail.com (Mike Jorgensen) Date: Tue Dec 22 12:32:13 2009 Subject: [Histonet] Antigen retrieval and myosin heavy chain question Message-ID: Dear Histonetters, I was wondering if anyone out there has used antigen retrieval methods to facilitate immuno-staining of formalin-fixed skeletal muscle fibers? I am using ALD-58 + ABC Elite Mouse kit to stain slow-tonic fibers in fresh amphibian muscles, but need to take a look at the fiber distribution of some rare museum specimens. Any suggestions are welcome. Thanks! -Mike -------------------------------------------------- Mike Jorgensen PhD Candidate Department of Biological Sciences Ohio University Athens, Ohio 45701 From Vickroy.Jim <@t> mhsil.com Tue Dec 22 13:44:54 2009 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Dec 22 13:45:02 2009 Subject: [Histonet] BK Virus Antibody Message-ID: <24A4826E8EF0964D86BC5317306F58A54254868CE4@mmc-mail.ad.mhsil.com> We used to purchase our BK virus antibody from a company called Chemicon. Apparently they were bought out by Millipore and the antibody is not available from them. I need to get the antibody from a new source. Any ideas? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From gayle.callis <@t> bresnan.net Tue Dec 22 14:04:52 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Dec 22 14:05:15 2009 Subject: [Histonet] Re: Trichrome stain for MMA and Sandersons Rapid Bone Stain chemical makeup (long reply with protocol) Message-ID: <000301ca8342$096c1980$1c444c80$@callis@bresnan.net> Jack Ratiff wrote: You can do H&E and VVG quite nicely on this type of MMA embedded tissue. Maybe you could also try a Sanderson's (methylene blue) with the Van Gieson (acid fuchsin w/ picric acid) counterstain??? On Dec 21, 2009, at 11:51 PM, Randall Carpenter <@t> usiwireless.com > wrote: > Dear Histonet, > > I was wondering what the best Trichrome stain might be for sawn and > ground sections of large stent/artery in methylmethacrylate. I am > also looking to stain elastic fibers. Any suggestions? Thanks. > > Randy Carpenter ******************************************* Several years ago I posted a discussion of Sanderson's bone stain is the same as Stevenels blue developed for bone work by Maniatopoulos C, Rodriguez A, Deporter DA, Melcher AH: An improved method for preparing histological sections of metallic implants. Internat J Oral & Maxillofacial Implants 1(1):31, 1987. The stain was originally developed as a parasite stain. Sanderson figured out an easier way to make up the staining solution than the original recipe, and then it was marketed by Surgipath with trademark. Her recipe is proprietary and stains with the same results as the Maniatopoulos method. We did the comparison in our lab and had identical results. Because the Stevenel's is such a royal pain to make in the lab, I suggest buying the Sanderson Bone stain. It is money well spent to avoid a long day of stain preparation. The chemistry of making this stain is interesting in that potassium permangante oxidizes methylene blue, forming a thick gooey ppt that takes a great deal of stirring and heating to get things into solution. The pH is very alkaline, somewhere in 9 or higher range and when using this stain, further heating of the solution the methylene blue will continue to oxidize and the pH continues to increase. We found our homemade Stevenels needed to be topped off frequently, and also filtered since a black ppt keeps forming. Conn's Biological stains Lillie, RD, revised by Stotz EH and Emmel,VM: H J Conns Biological Stains. pp 423-424, Ninth ed., Williams and Wilkins Co, Baltimore MD 1977 has explanation of what happens to the methylene blue when oxidized by KMNO4. The by- products of the methylene blue oxidation are toluidine blue, methylene violet, thionin, Azure A and other Azures along with residual methylene blue left in the solution. Some dyes are often found in formulations/recipes for PMMA embedded bone sections e.g. toluidine blue. Staining results are Osteoid - blue to intense blue-green; Muscle, connective tissue - blue to blue-green; Cartilage - blue and/or shades of violet to purple; Calcified cartilage - medium to dark purple Calcified bone is stained by acid fuchsin, and light green can also be used. Basic Fuchsin is also a counterstain Since PMMA is very hydrophobic, only low molecular weight dyes penetrate the plastic sufficiently in order to stain all the the described components. Acid fuchsin also has a low molecular weight. RW Horobin has a wonderful publication on the effects of staining on plastics. I am not putting my finger on the reference, but this paper alone is an education on how dyes work on plastic embedded tissues, including PMMA and GMA. Some important factors that affect staining of PMMA embedded bone are Low molecular weight dyes Temperature - must be heated pH - alkaline is important for good staining. These factors also affect how hydrophilic plastics for EM and other methacrylate embedded tissues stain. It is well established that toluidine blue, in sodium borate at pH 11 is a stain of choice for EM embedded tissues, and requires heating. Hopefully this will help in understanding the mechanism. I suggest accessing the publications for further information. Personally, I have preferred MacNeals Tetrachrome over Sandersons/Stevenels for undecalcified bone in MMA, ground sections but the surface must be etched with 0.7% formic acid and also alcohol etching of MMA, rinsed well, dried then immersed into the MacNeals. Do not buy MacNeals as a commercial preparation, it does not work as well as an in house preparation. Here is the protocol for MacNeals with a basic fuchsin counterstain. MacNeals can also be combined with Toluidine blue for a brilliant, well stained bone section in MMA. As in all staining, young bone takes less time to stain, old bone longer, acid etching enhances calcified staining by allowing stain to penetrate into a few micrometers of bone section surface. This is actually a mild surface decalcification whereas the alcohol etching softens and probably removes a very slight amount of the MMA. One can always play with staining times, but do NOT acid etch too long or you will have a section that is too overstained. MacNeals has also been combined with Toluidine blue for a very nice stain combination. Basic fuchsin on acid etched bone will be very red, so one cannot be heavy handed with this stain nor van Giesons. MACNEAL'S TETRACHROME STOCK SOLUTION 0.5g methylene blue 0.8g azure a eosinate 0.1g methylene violet 250 ml methanol 250 glycerin Stir, leave at 50C for 12 hours, then 37C for 3 days. Filter, store in dark. WORKING STAINING SOLUTION 5 mls stock stain solution, 95mls distilled water PROCEDURE: MODIFIED McNEAL TETRACHROME Grind and polish surface up to l micrometer alumina. Rinse well with tap water. 1. Etch surface with 0.7% formic acid for l minute 2. Rinse with tap water 3. 100% ethanol for 10 minutes, sonicate 4. Stain in Modified McNeal for 5 minute or longer. If overstaining with MacNeals occurs, dilute acid alcohol removes stain. 5. Rinse with distilled water 6. Differentiate with 70% ethanol with 1 dip 7. 0.1% Aq. Basic Fuchsin 15-30 seconds 8. Brief distiled water rinse 9. Air dry, do not coverslip. To view, put a white paper under slide, turn light up to brightest level without filters on microscope, and view stained surface by placing a cover slip on top of stained section. Mounting coverslip will crack the MMA. Gayle Callis HTL/HT/MT(ASCP) From gayle.callis <@t> bresnan.net Tue Dec 22 14:09:12 2009 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Dec 22 14:09:25 2009 Subject: [Histonet] Happy Holidays Message-ID: <000801ca8342$a2092930$e61b7b90$@callis@bresnan.net> To all my friends and colleagues on Histonet I wish you a very Happy Holiday season filled with family and friends Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT From kalschev <@t> svm.vetmed.wisc.edu Tue Dec 22 14:10:28 2009 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Tue Dec 22 14:10:40 2009 Subject: [Histonet] Trichrome/PMMA ground sections Message-ID: <005401ca8342$ceb6d810$c5d76880@vetmed.wisc.edu> Randy, Our researchers like the modified Goldner's Trichrome. I used a paraffin method and modified it to work for plastic. A Toluidine Blue works well on stents, plus the other stains discussed earlier. Vicki From anonwums1 <@t> gmail.com Tue Dec 22 15:14:36 2009 From: anonwums1 <@t> gmail.com (Adam .) Date: Tue Dec 22 15:14:42 2009 Subject: [Histonet] Directly conjugated secondaries versus biotinylated secondaries Message-ID: <858249120912221314q669579b2jcc3aa8b97e56a545@mail.gmail.com> Greetings, Hopefully all of you are enjoying a great time with your friends and family this week rather than working like me. Here is my problem. I have an antibody (anti-panendothelial antigen, i.e. MECA32 from BD http://www.bdbiosciences.com/ptProduct.jsp?prodId=19506) and I'm trying to get it to work on 4% PFA fixed, decalcified, paraffin embedded sections. I previously titered it to 2 ug / mL with a biotinylated anti-rat F(ab')2 (1 ug / mL) and then a strepavidin Dylight 594 (1 ug / mL) and got beautiful, strong staining. Those of you who have been helping me from my IHC infancy would be proud. However, I really need it to be a directly conjugated secondary because I want to co-stain with something else that only works with a biotinylated secondary followed by tyramide amplification. So I retitered it from 5 ug / mL and down and came in with a Dylight-649 conjugated anti-rat (1 ug / mL) secondary and I "saw" nothing (Dylight 649, which is a Cy5 replacement, is too far red to see without the aid of a camera--although the guy who helped me use the microscope says there's one woman on campus who has infrared vision). I should note that another group has reported getting this to work with a biotinylated primary and adding an fluorophore conjugated avidin. I can think of a couple of possibilities here 1) The antibody needs that extra edge of a biotinylation to get sufficient signal (has anyone ever seen this problem which couldn't be solved by titrating something?) 2) I need to increase the primary titer ever more 3) I need to increase the secondary titer (what primary titer should I use if I do this?) 4) Dylight 649 is just too dim to see anything and if I switched to another fluor, it would work. I have no experience with this fluor, as our floor scope doesn't have the filters for it. 5) This is all a fluke and I screwed something up Any suggestions on how to troubleshoot this are welcome. Adam From MAUGER <@t> email.chop.edu Wed Dec 23 08:12:08 2009 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Wed Dec 23 08:13:34 2009 Subject: [Histonet] RE: BK Virus Antibody In-Reply-To: <24A4826E8EF0964D86BC5317306F58A54254868CE4@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A54254868CE4@mmc-mail.ad.mhsil.com> Message-ID: <443F5B475A9BF647AB962E834884EBAD2657100FC1@EX7CCRPW03V1.chop.edu> Hi Jim, We use the SV40(large T-antigen) antibody from BDBioscience #554154. Vector has a different antibody for polyomavirusJC/BK viruses, #VP-P973. Both work well for us in FFPE. The problem we always have is no positive control tissue! Good luck. Jo Joanne Mauger ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim [Vickroy.Jim@mhsil.com] Sent: Tuesday, December 22, 2009 2:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] BK Virus Antibody We used to purchase our BK virus antibody from a company called Chemicon. Apparently they were bought out by Millipore and the antibody is not available from them. I need to get the antibody from a new source. Any ideas? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andreas.kappeler <@t> pathology.unibe.ch Wed Dec 23 08:59:53 2009 From: andreas.kappeler <@t> pathology.unibe.ch (Kappeler, Andreas) Date: Wed Dec 23 09:04:01 2009 Subject: [Histonet] AW: BK Virus Antibody In-Reply-To: <24A4826E8EF0964D86BC5317306F58A54254868CE4@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A54254868CE4@mmc-mail.ad.mhsil.com> Message-ID: <4EAFACC375BF974C8A57D33444CEE288029C1702@AAI-MBX3.campus.unibe.ch> Hi James we use mo-a-SV40 T Ag monoclonal antibody, clone PAb 416, which is known to cross-react with BK virus (Mann & Carroll; Virology, 1984; 138(2):379-85). The mAb used to be from Oncogene, which now is Merck. CatNo DP02. Working conc. is 2 ug/ml, pretreatment HIER high pH. Hope this helps. Best regards Andi Kappeler Institute of Pathology, University of Bern, Switzerland ________________________________________ Von: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] im Auftrag von Vickroy, Jim [Vickroy.Jim@mhsil.com] Gesendet: Dienstag, 22. Dezember 2009 20:44 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] BK Virus Antibody We used to purchase our BK virus antibody from a company called Chemicon. Apparently they were bought out by Millipore and the antibody is not available from them. I need to get the antibody from a new source. Any ideas? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bonnie.Whitaker <@t> osumc.edu Wed Dec 23 09:09:18 2009 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Wed Dec 23 09:09:23 2009 Subject: [Histonet] Sunquest/CoPath question Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F60D669BF@msxc06.OSUMC.EDU> Hi All, Will anyone using Sunquest/CoPath please contact me? I am trying to improve the level of accuracy with our Part Types, and I wondered how others are addressing specimens that arrive en bloc, but can be separated out for billing purposes. Thanks, and Happy Holidays! Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center 614.293.5048 From Sarah.Boyd <@t> tengion.com Wed Dec 23 09:53:24 2009 From: Sarah.Boyd <@t> tengion.com (Sarah Boyd) Date: Wed Dec 23 09:53:29 2009 Subject: [Histonet] WT1 antibody for suspended whole glomeruli Message-ID: <26CBED9658AAC7428834C0E0DC442D8F161D4517@EXSERVER.Tengion.local> I was wondering if anyone knew of a good antibody for WT1 to use on whole fixed rat glomeruli in suspension with IF. We have a working Ab for Western blots and tissue sections, but I cannot seem to get anything to work on the suspension preps. I have tried Abcam, Santa Cruz, and Dako. Any help would be appreciated Sarah Boyd Research Associate Tengion From jkiernan <@t> uwo.ca Wed Dec 23 10:24:18 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Dec 23 10:24:21 2009 Subject: [Histonet] Sanderson's rapid bone stain + Acid fuchsin Message-ID: Cathy Sanderson wrote a review article about staining undecalcified bone only 11 years ago (which is recent in the literature of histological techniques): Sanderson, C. (1997) Entering the realm of mineralized bone processing: A review of the literature and techniques. J. Histotechnol. 20:259-266. If you're a member of the NSH you can request PDF files from the journal by way of the society's web site. (I hope this is still so; I haven't requested one in the last year.) There is an excellent chapter on bonology by Gayle Callis in Bancroft, J.D., and Gamble, M. (eds). Theory and Practice of Histological Techniques: London: Churchill Livingstone. It's Chapter 14 in the 5th edition (2002). I don't have the 6th (2008) edition; chapter numbers may not be the same. For discussion of the principles involved in staining sections of plastic-embedded tissue, see: Horobin RW (1982) Histochemistry: An Explanatory Outline of Histochemistry and Biophysical Staining. Gustav Fischer, Stuttgart. Horobin RW (1988) Understanding Histochemistry: Selection, Evaluation and Design of Biological Stains. Ellis Horwood, Chichester. Acid fuchsine and basic fuchsine have the same colour but almost opposite staining properties, which have been quite well understood since about 1880. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: sklan@illinois.edu Date: Monday, December 21, 2009 14:26 Subject: [Histonet] Sanderson's rapid bone stain + Acid fuchsin To: histonet@lists.utsouthwestern.edu > Hi All - > > I am trying to figure out if the combination of Sanderson's > rapid bone stain and acid fuchsin as the counterstain (or > methylene blue + basic fuchsin) results in pink/red staining of > biologically formed apatite (apatite not associated with > collagen produced by osteoblasts)? An associated question > is if the dye-tissue interaction between acid or basic fuchsin > and mineralized bone is due to dye-collagen interactions? > > If anyone has any experiences or references that may help, > please let me know. > > Thank you for your help, > > Sheeny > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Wed Dec 23 10:49:16 2009 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Wed Dec 23 10:49:21 2009 Subject: [Histonet] FW: The results of your email commands Message-ID: <5680DA93771F0C48954CC8D38425E72401AB34FC@ISMAIL.parrishmed.local> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Tuesday, December 22, 2009 12:16 PM To: Hannen, Valerie Subject: The results of your email commands The results of your email command are provided below. Attached is your original message. - Results: Ignoring non-text/plain MIME parts - Unprocessed: =20 I was wondering if anyone has an idea where I can get some fine abrasive QUICK!! Our vendor has told us that it is a "special in and out item" and that it will take 2 -4 weeks to get it. They are saying we can't get any until the end of January. The funny thing is that we ordered the 3rd day of December...and now they say we can't get until the end of January? That is not 2-4 weeks...that is more like 4-8 weeks. We are down to maybe are down to 1/16 or 1/8 of a bottle. HELP!! =20 Thanks in advance for any help that can be given. =20 Valerie Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville,Floirida ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or - Ignored: copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** - Done. ************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ************************************************************** From brian <@t> prometheushealthcare.com Wed Dec 23 11:53:07 2009 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Wed Dec 23 11:53:17 2009 Subject: [Histonet] Lab Manager Needed for Histology and Cytology Message-ID: <003901ca83f8$c9084190$5b18c4b0$@com> We are looking for a lab manager to manage both Histology and Cytology. It is a new opening with a lab that specializes in Uropathology. The position is located in Richmond, VA. Please email your resume today for immediate consideration. Happy Holidays! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!*** http://twitter.com/PrometheusBlog From thomas.crowell <@t> novartis.com Wed Dec 23 12:07:00 2009 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Wed Dec 23 12:07:30 2009 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 12/23/2009 and will not return until 01/05/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. From baustin <@t> cbgbiotech.com Wed Dec 23 12:10:02 2009 From: baustin <@t> cbgbiotech.com (Baustin) Date: Wed Dec 23 12:10:06 2009 Subject: [Histonet] Auto Reply Message-ID: <999778625@mail-server.cbgbiotech.com> Please do not respond to this automatic e-mail reply. I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies@cbgbiotech.com or fax it to 614-863-1676 Attn Ordering. If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225. Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG. From NMargaryan <@t> childrensmemorial.org Wed Dec 23 12:55:19 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Dec 23 12:57:19 2009 Subject: [Histonet] IF staining on FFPE tissue Message-ID: Dear Histonetters, Hopefully you are enjoying a great Holidays with your friends and family! I have been asked to perform . I got positive and negative absolutely identical with beautiful nuclear DAPI and very red RBC. What to do or what to change in my protocol to avoid RBC and background? Here is my protocol: 1.Deparaffinization (xyline- 60min, 100%Eth- 15min, 95%- 5min, 70%- 5min, H2O) 2. Antigen Retrieval ph6 in Citrate buffer (same I use for IHC) 3. Wash H2O and TBST 4. Protein block- 10minhttps://webmail.childrensmemorial.org/owa/?ae=PreFormAction&t=IPM.Note&a=Reply&id=RgAAAADBaOhrx0l9S5XoN3P8OXzpBwDbYZXQd%2bZ%2fQIud7XQY1kjHAAAKzOB%2bAADBupMEDGuaSo2Eh4%2bT%2fsNqAAOO6ORIAAAJ# 5. Ab, Rb anti-human - 60-90 min (same I use for IHC) 6. Wash TBST -5-10min 7. secondary Donkey anti-Rb 594 red - 60min 8. Wash TBST -5-10min, H2O -5min 9. DAPI - 10min 10. Wash H2O -5-10min 11. Gelvatol coverslip Am I using wrong secondary? Is there any specific secondary or protocol for IF staining for FFPE to avoid background? I just used same AR and primary Ab's dilution like i use for IHC with nice results. Any suggestions are appreciated, especially in these Holidays days from working histo- people:) Thanks and have a warm and nice Holidays, Naira From NMargaryan <@t> childrensmemorial.org Wed Dec 23 12:56:23 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Dec 23 13:00:05 2009 Subject: [Histonet] RE: IF staining on FFPE tissue In-Reply-To: References: Message-ID: Dear Histonetters, Hopefully you are enjoying a great Holidays with your friends and family! I have been asked to perform . I got positive and negative absolutely identical with beautiful nuclear DAPI and very red RBC. What to do or what to change in my protocol to avoid RBC and background? Here is my protocol: 1.Deparaffinization (xyline- 60min, 100%Eth- 15min, 95%- 5min, 70%- 5min, H2O) 2. Antigen Retrieval ph6 in Citrate buffer (same I use for IHC) 3. Wash H2O and TBST 4. Protein block- 10min 5. Ab, Rb anti-human - 60-90 min (same I use for IHC) 6. Wash TBST -5-10min 7. secondary Donkey anti-Rb 594 red - 60min 8. Wash TBST -5-10min, H2O -5min 9. DAPI - 10min 10. Wash H2O -5-10min 11. Gelvatol coverslip Am I using wrong secondary? Is there any specific secondary or protocol for IF staining for FFPE to avoid background? I just used same AR and primary Ab's dilution like i use for IHC with nice results. Any suggestions are appreciated, especially in these Holidays days from working histo- people:) Thanks and have a warm and nice Holidays, Naira From Kim.Donadio <@t> bhcpns.org Thu Dec 24 09:03:58 2009 From: Kim.Donadio <@t> bhcpns.org (Kim.Donadio@bhcpns.org) Date: Thu Dec 24 09:04:17 2009 Subject: [Histonet] Hello fellow Histo's , 2 questions for you Message-ID: First of all I hope all of you are enjoying your Holiday. And I expect that many of you are not at work like me, but when you get a chance if you could help me out by answering these two questions I would be very grateful. :-) 1) In your lab when you receive renal biopsy's fresh. Who finds the Glomeruli using a microscope? A pathologist or a Histologist? 2) Also, if you have frozen sections off site, do you always send a Histologist to assist the Pathologist? Thanks for your help in advance Many Blessing to each of you! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. From rjbuesa <@t> yahoo.com Thu Dec 24 09:18:31 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 24 09:18:37 2009 Subject: [Histonet] Hello fellow Histo's , 2 questions for you In-Reply-To: Message-ID: <404178.71779.qm@web65709.mail.ac4.yahoo.com> Dear Kim: Although histology is practices very similarly all around the world, each histolab operates with its own rules because there are no general rules. 1- who finds the glomerulii? Usually is a pathologist, but some have trained a histology technologist that takes care of the DIF or to prepare the samples for TEM and the pathologist task is delegated to that histotech. So, it depends on the pathology director to define how the work is done. 2- off site frozen sections??sometimes a histotech is sent to help the pathologist, but usually the pathologist takes care of the whole thing. So, again, it depends on the site although the custom is: 1- glomerulio are identified by the PT, and 2- PT take care of off site frozens. Hope this will help you Happy Holidays Ren? J. --- On Thu, 12/24/09, Kim.Donadio@bhcpns.org wrote: From: Kim.Donadio@bhcpns.org Subject: [Histonet] Hello fellow Histo's , 2 questions for you To: histonet@lists.utsouthwestern.edu Date: Thursday, December 24, 2009, 10:03 AM First of all I hope all of you are enjoying your Holiday. And I expect that many of you are not at work like me, but when you get a chance if you could help me out by answering these two questions I would be very grateful. :-) 1) In your lab when you receive renal biopsy's? fresh. Who finds the Glomeruli using a microscope? A pathologist or a Histologist? 2) Also, if you have frozen sections off site, do you always send a Histologist to assist the Pathologist? Thanks for your help in advance Many Blessing to each of you! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message.? For questions, contact the BHC Privacy Officer at (850) 434-4472.? Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From baustin <@t> cbgbiotech.com Thu Dec 24 12:07:56 2009 From: baustin <@t> cbgbiotech.com (Baustin) Date: Thu Dec 24 12:07:59 2009 Subject: [Histonet] Auto Reply Message-ID: <999844092@mail-server.cbgbiotech.com> Please do not respond to this automatic e-mail reply. I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies@cbgbiotech.com or fax it to 614-863-1676 Attn Ordering. If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225. Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG. From WBENTON <@t> umm.edu Thu Dec 24 12:14:31 2009 From: WBENTON <@t> umm.edu (Walter Benton) Date: Thu Dec 24 12:14:49 2009 Subject: [Histonet] Renal Question In-Reply-To: <437633B4.254@GWIA2.umm.edu> References: <437633B4.254@GWIA2.umm.edu> Message-ID: <4B336934.D886.00F4.3@umm.edu> Kim, In response to your 1st question our Pathologist along with the surgeon generally look under the scope together at the specimen, never a histotech. In response to the 2nd question, regardless of location we do not utilize the histotech for help. PAs or Residents handle all frozens. Our true off site cases are sent via image analysis over the internet, which are read by the Pathologist. Hope this helps. Message: 5 Date: Thu, 24 Dec 2009 09:03:58 -0600 From: Kim.Donadio@bhcpns.org Subject: [Histonet] Hello fellow Histo's , 2 questions for you To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" First of all I hope all of you are enjoying your Holiday. And I expect that many of you are not at work like me, but when you get a chance if you could help me out by answering these two questions I would be very grateful. :-) 1) In your lab when you receive renal biopsy's fresh. Who finds the Glomeruli using a microscope? A pathologist or a Histologist? 2) Also, if you have frozen sections off site, do you always send a Histologist to assist the Pathologist? Thanks for your help in advance Many Blessing to each of you! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 Walter Benton, HT(ASCP)QIHC Histology Supervisor University of Maryland Medical Center Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 >>> On 12/24/2009 at 1:06 PM, wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Thomas Crowell is out of the office. (thomas.crowell@novartis.com) 2. Auto Reply (Baustin) 3. IF staining on FFPE tissue (Margaryan, Naira) 4. RE: IF staining on FFPE tissue (Margaryan, Naira) 5. Hello fellow Histo's , 2 questions for you (Kim.Donadio@bhcpns.org) 6. Hello fellow Histo's , 2 questions for you (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 23 Dec 2009 13:07:00 -0500 From: thomas.crowell@novartis.com Subject: [Histonet] Thomas Crowell is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 12/23/2009 and will not return until 01/05/2010. Please contact Kelly Miner at 617-871-5122 if you have any questions regarding clinical trial samples. ------------------------------ Message: 2 Date: Wed, 23 Dec 2009 13:10:02 -0500 From: Baustin Subject: [Histonet] Auto Reply To: Message-ID: <999778625@mail-server.cbgbiotech.com> Content-Type: text/plain; charset="utf-8" Please do not respond to this automatic e-mail reply. I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies@cbgbiotech.com or fax it to 614-863-1676 Attn Ordering. If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225. Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG. ------------------------------ Message: 3 Date: Wed, 23 Dec 2009 12:55:19 -0600 From: "Margaryan, Naira" Subject: [Histonet] IF staining on FFPE tissue To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Histonetters, Hopefully you are enjoying a great Holidays with your friends and family! I have been asked to perform . I got positive and negative absolutely identical with beautiful nuclear DAPI and very red RBC. What to do or what to change in my protocol to avoid RBC and background? Here is my protocol: 1.Deparaffinization (xyline- 60min, 100%Eth- 15min, 95%- 5min, 70%- 5min, H2O) 2. Antigen Retrieval ph6 in Citrate buffer (same I use for IHC) 3. Wash H2O and TBST 4. Protein block- 10minhttps://webmail.childrensmemorial.org/owa/?ae=PreFormAction&t=IPM.Note&a=Reply&id=RgAAAADBaOhrx0l9S5XoN3P8OXzpBwDbYZXQd%2bZ%2fQIud7XQY1kjHAAAKzOB%2bAADBupMEDGuaSo2Eh4%2bT%2fsNqAAOO6ORIAAAJ# 5. Ab, Rb anti-human - 60-90 min (same I use for IHC) 6. Wash TBST -5-10min 7. secondary Donkey anti-Rb 594 red - 60min 8. Wash TBST -5-10min, H2O -5min 9. DAPI - 10min 10. Wash H2O -5-10min 11. Gelvatol coverslip Am I using wrong secondary? Is there any specific secondary or protocol for IF staining for FFPE to avoid background? I just used same AR and primary Ab's dilution like i use for IHC with nice results. Any suggestions are appreciated, especially in these Holidays days from working histo- people:) Thanks and have a warm and nice Holidays, Naira ------------------------------ Message: 4 Date: Wed, 23 Dec 2009 12:56:23 -0600 From: "Margaryan, Naira" Subject: [Histonet] RE: IF staining on FFPE tissue To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Histonetters, Hopefully you are enjoying a great Holidays with your friends and family! I have been asked to perform . I got positive and negative absolutely identical with beautiful nuclear DAPI and very red RBC. What to do or what to change in my protocol to avoid RBC and background? Here is my protocol: 1.Deparaffinization (xyline- 60min, 100%Eth- 15min, 95%- 5min, 70%- 5min, H2O) 2. Antigen Retrieval ph6 in Citrate buffer (same I use for IHC) 3. Wash H2O and TBST 4. Protein block- 10min 5. Ab, Rb anti-human - 60-90 min (same I use for IHC) 6. Wash TBST -5-10min 7. secondary Donkey anti-Rb 594 red - 60min 8. Wash TBST -5-10min, H2O -5min 9. DAPI - 10min 10. Wash H2O -5-10min 11. Gelvatol coverslip Am I using wrong secondary? Is there any specific secondary or protocol for IF staining for FFPE to avoid background? I just used same AR and primary Ab's dilution like i use for IHC with nice results. Any suggestions are appreciated, especially in these Holidays days from working histo- people:) Thanks and have a warm and nice Holidays, Naira ------------------------------ Message: 5 Date: Thu, 24 Dec 2009 09:03:58 -0600 From: Kim.Donadio@bhcpns.org Subject: [Histonet] Hello fellow Histo's , 2 questions for you To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain;charset="US-ASCII" First of all I hope all of you are enjoying your Holiday. And I expect that many of you are not at work like me, but when you get a chance if you could help me out by answering these two questions I would be very grateful. :-) 1) In your lab when you receive renal biopsy's fresh. Who finds the Glomeruli using a microscope? A pathologist or a Histologist? 2) Also, if you have frozen sections off site, do you always send a Histologist to assist the Pathologist? Thanks for your help in advance Many Blessing to each of you! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ------------------------------ Message: 6 Date: Thu, 24 Dec 2009 07:18:31 -0800 (PST) From: Rene J Buesa Subject: [Histonet] Hello fellow Histo's , 2 questions for you To: histonet@lists.utsouthwestern.edu, Kim.Donadio@bhcpns.org Message-ID: <404178.71779.qm@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear Kim: Although histology is practices very similarly all around the world, each histolab operates with its own rules because there are no general rules. 1- who finds the glomerulii? Usually is a pathologist, but some have trained a histology technologist that takes care of the DIF or to prepare the samples for TEM and the pathologist task is delegated to that histotech. So, it depends on the pathology director to define how the work is done. 2- off site frozen sections??sometimes a histotech is sent to help the pathologist, but usually the pathologist takes care of the whole thing. So, again, it depends on the site although the custom is: 1- glomerulio are identified by the PT, and 2- PT take care of off site frozens. Hope this will help you Happy Holidays Ren? J. --- On Thu, 12/24/09, Kim.Donadio@bhcpns.org wrote: From: Kim.Donadio@bhcpns.org Subject: [Histonet] Hello fellow Histo's , 2 questions for you To: histonet@lists.utsouthwestern.edu Date: Thursday, December 24, 2009, 10:03 AM First of all I hope all of you are enjoying your Holiday. And I expect that many of you are not at work like me, but when you get a chance if you could help me out by answering these two questions I would be very grateful. :-) 1) In your lab when you receive renal biopsy's? fresh. Who finds the Glomeruli using a microscope? A pathologist or a Histologist? 2) Also, if you have frozen sections off site, do you always send a Histologist to assist the Pathologist? Thanks for your help in advance Many Blessing to each of you! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message.? For questions, contact the BHC Privacy Officer at (850) 434-4472.? Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 73, Issue 33 **************************************** This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From turkekul <@t> gmail.com Thu Dec 24 12:19:47 2009 From: turkekul <@t> gmail.com (mesruh turkekul) Date: Thu Dec 24 12:19:53 2009 Subject: [Histonet] Re: Histonet Digest, Vol 73, Issue 33 In-Reply-To: <4b33ac3f.2708c00a.3604.fffff4eaSMTPIN_ADDED@mx.google.com> References: <4b33ac3f.2708c00a.3604.fffff4eaSMTPIN_ADDED@mx.google.com> Message-ID: Hi Naira, The RBCs have a heme groups that have very high autofluorescence. They will glow with every filter in every color. They will glow even without any staining. Just try to image unstained section and you will see. "*Autofluorescence* is the fluorescenceof other substances than the fluorophore of interest. It increases the background signal.Autofluorescence can be problematic in fluorescence microscopy . In most fluorescence microscopy, fluorescent stains (such as fluorescently-labeled antibodies ) are applied to samples to stain specific structures. Autofluorescence interferes with detection of the resulting specific fluorescent signals, especially when the signals of interest are very dim ? it causes structures other than those of interest to become visible. Depending upon the shape of the structures of interest and the other structures, it may not be obvious that this has occurred. In some microscopes (mainly confocal microscopes), it is possible to make use of different lifetime of the excited states of the added fluorescent markers and the endogenous molecules to exclude most of the autofluorescence." Unfortunately there is no way to avoid that. If you check online you may find people using: sodium borhydrate, 0.1M Glycine pH=3, sudan black or many other reagents to prevent autofluoresence but the success rate is very low. You may try to get rid of the red signal of the RBC with the imaging software. They have different spectra than Alexa594 and you can do spectral separation. Happy holidays! Mesru From JWatson <@t> gnf.org Thu Dec 24 12:33:27 2009 From: JWatson <@t> gnf.org (James Watson) Date: Thu Dec 24 12:33:33 2009 Subject: [Histonet] Re: Histonet Digest, Vol 73, Issue 33 In-Reply-To: References: <4b33ac3f.2708c00a.3604.fffff4eaSMTPIN_ADDED@mx.google.com> Message-ID: We routinely quench RBC auto-fluorescence with the following procedure: 10 mM Copper Sulfate 10 mM Copper Sulfate Cupric Sulfate...........................................1.25 gm 50 mM Amonium acetate (pH5)....................500.0 ml Adjust ph to 5.0 with 1.0 M NaOH 50 mM Ammonium acetate (pH5) Ammonium acetate.....................................1.93 gm Distilled water............................................500.0 ml Adjust pH to 5.0 with 1.0 M HCl 1. After staining wash slides in Reaction Buffer 5 times for 10 minutes each. 2. Rinse in PBS 2 times for 10 minutes each. 3. Rinse in distilled water 5 minutes. 4. Place slides in 10mM copper sulfate for 8 minutes. 5. Return slides to distilled water and check for autofluorescence with microscope. 6. if needed return slides to 10mM copper sulfate for a couple of more minutes and check again. 7. Rinse slides for 5 minutes in distilled water. 8. Rinse in PBS 2 times for 10 minutes each. 9. Coverslip slides with appropriate mounting media. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mesruh turkekul Sent: Thursday, December 24, 2009 10:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 73, Issue 33 Hi Naira, The RBCs have a heme groups that have very high autofluorescence. They will glow with every filter in every color. They will glow even without any staining. Just try to image unstained section and you will see. "*Autofluorescence* is the fluorescenceof other substances than the fluorophore of interest. It increases the background signal.Autofluorescence can be problematic in fluorescence microscopy . In most fluorescence microscopy, fluorescent stains (such as fluorescently-labeled antibodies ) are applied to samples to stain specific structures. Autofluorescence interferes with detection of the resulting specific fluorescent signals, especially when the signals of interest are very dim - it causes structures other than those of interest to become visible. Depending upon the shape of the structures of interest and the other structures, it may not be obvious that this has occurred. In some microscopes (mainly confocal microscopes), it is possible to make use of different lifetime of the excited states of the added fluorescent markers and the endogenous molecules to exclude most of the autofluorescence." Unfortunately there is no way to avoid that. If you check online you may find people using: sodium borhydrate, 0.1M Glycine pH=3, sudan black or many other reagents to prevent autofluoresence but the success rate is very low. You may try to get rid of the red signal of the RBC with the imaging software. They have different spectra than Alexa594 and you can do spectral separation. Happy holidays! Mesru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annigyg <@t> gmail.com Thu Dec 24 13:12:48 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Dec 24 13:12:53 2009 Subject: [Histonet] Hello fellow Histo's , 2 questions for you In-Reply-To: <404178.71779.qm@web65709.mail.ac4.yahoo.com> References: <404178.71779.qm@web65709.mail.ac4.yahoo.com> Message-ID: my techs always tell the interventional radiologists if there are gloms in the renals and whether to take another core of tissue - the pathologists are always 'far too busy' to attend at the renal biopsies!! we never have off site frozens (just as well - maybe they would send us to do those as well) ha ha christmas joke have a good one AbuDhabiAnnie 2009/12/24 Rene J Buesa > Dear Kim: > Although histology is practices very similarly all around the world, each > histolab operates with its own rules because there are no general rules. > 1- who finds the glomerulii? Usually is a pathologist, but some have > trained a histology technologist that takes care of the DIF or to prepare > the samples for TEM and the pathologist task is delegated to that histotech. > So, it depends on the pathology director to define how the work is done. > 2- off site frozen sections? sometimes a histotech is sent to help the > pathologist, but usually the pathologist takes care of the whole thing. So, > again, it depends on the site although the custom is: > 1- glomerulio are identified by the PT, and > 2- PT take care of off site frozens. > Hope this will help you > Happy Holidays > Ren? J. > > --- On Thu, 12/24/09, Kim.Donadio@bhcpns.org > wrote: > > > From: Kim.Donadio@bhcpns.org > Subject: [Histonet] Hello fellow Histo's , 2 questions for you > To: histonet@lists.utsouthwestern.edu > Date: Thursday, December 24, 2009, 10:03 AM > > > First of all I hope all of you are enjoying your Holiday. And I expect > that many of you are not at work like me, but when you get a chance if you > could help me out by answering these two questions I would be very > grateful. :-) > > 1) In your lab when you receive renal biopsy's fresh. Who finds the > Glomeruli using a microscope? A pathologist or a Histologist? > > 2) Also, if you have frozen sections off site, do you always send a > Histologist to assist the Pathologist? > > Thanks for your help in advance > > Many Blessing to each of you! > > > Kim Donadio > Pathology Supervisor > Baptist Hospital > 1000 W Moreno St. > Pensacola FL 32501 > Phone (850) 469-7718 > Fax (850) 434-4996 > > ----------------------------------------- > All electronic data transmissions originating from or sent to > Baptist Health Care Corporation (BHC) are subject to monitoring. > This message along with any attached data, are the confidential and > proprietary communications of BHC and are intended to be received > only by the individual or individuals to whom the message has been > addressed. If the reader of this message is not the intended > recipient, please take notice that any use, copying, printing, > forwarding or distribution of this message, in any form, is > strictly prohibited and may violate State or Federal Law. If you > have received this transmission in error, please delete or destroy > all copies of this message. For questions, contact the BHC Privacy > Officer at (850) 434-4472. Rev.10/07. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From carl.hobbs <@t> kcl.ac.uk Thu Dec 24 17:08:55 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Thu Dec 24 17:10:42 2009 Subject: [Histonet] Hello fellow Histo's , 2 questions for you Message-ID: <11D9615B89C10747B1C985966A63D7CA2C434CE8DB@KCL-MAIL04.kclad.ds.kcl.ac.uk> Rene's comments are most appropriate. Respect. When I was in Clinical Histopath ( a technician) at two different well-known London major Hospitals, it would be the technician who would be in the theatre when renal bxs were taken. EG; the person who took the biopsy would pass the specimen to me to microscopically confirm that it was in fact renal cortex. ( several times it would be mesenteric fat with, sometimes, a tiny piece of cortex but no Gloms: a horrible situation to be in as I would have to ask for another bx: I never cared for the Bx taker's opinion but I always felt very baad for the patient.) However, one must appreciate that taking good/appropriate kidney bxs is a great skill, imho: the renal capsule ( Glisson's) could be hard to penetrate. If the bx core was appropriate I would then sample the core for snap-freezing/pwax/TEM, depending upon the core ( with some advanced illness patients, one would have to work very hard to establish that the bx would be meaningful, diagnostically). Yes, for TEM I would microscopically dissect out one or more glom. depending upon the bx. No Pathologists were involved but all technicians were rigorously trained...( by experienced technicians;-) I would do the equivilent for Quad muscle biopsies....and also for SI bxs for Coeliac's disease. As a Technician, having to make such decisions was always very stressful for me as I was not trained to "distance " myself from the patient: I always empathised with the stress of the patient, particularly when the patient was a little kid or a kid/ adult who was very ill. I can still recall them "whimpers" of distress. Offsite bxs? Same applied. No Pathologist involved. This never resulted in any problems, the 10 years I did this. No comments in this email are relevant to current Practices and are reflections of my personal experience. I wish all Histonetters a Happy Holidays/Xmas/Christmas! And...success in the Christian New Year. Most importantly, I wish the Initiators/Organisers/Maintainers of Histonet the same. I am most grateful for this site. It is an invaluable resource and yet still refreshingly simple , in this age of "bloated websites" Great Respect. Carl From hisham.sys <@t> gmail.com Fri Dec 25 00:05:42 2009 From: hisham.sys <@t> gmail.com (Hisham Mohammed) Date: Fri Dec 25 00:05:46 2009 Subject: [Histonet] cleaning of thermoshandon knife sharpener plates Message-ID: <3b7d8d520912242205u68c3e92aw98f5e74fdb0a5227@mail.gmail.com> dear all i would like to know what solution do people use to clean the plates of thermoshandon knife/blade sharpener following the process of sharpening using the diamond particle containing pastes. the company doesnt tell about the nature of the solution provided. wishing a merry Xmas and happy new year to all histonetters hisham mohammed senior research fellow nbrc, india From aazath <@t> hotmail.com Fri Dec 25 08:12:45 2009 From: aazath <@t> hotmail.com (Aazath Raj) Date: Fri Dec 25 08:12:49 2009 Subject: [Histonet] Processing Of CSF Message-ID: Dear Friends, Iam a Technical Officer in Apollo Hospitals India in Department of Histopathology and Cytology. I am having a problem in CSF processing for cytology. We are using Thermo Shandon 4- Cytocentrifuge for processing CSF and Urine. The yield is very less. We spin it with 750rpm ( acceleration- medium)for 5 min with 1drop of egg albumin as adisive.Most of the time the CSF has been reported as no cellular material found.Iam little concern about the technique ,which we are following. Is anybody have a better way to process the CSF for Cytology with better yield of material. Merry X-Mas and Happy New Year to all. with regards, Aazath _________________________________________________________________ Windows 7: Find the right PC for you. Learn more. http://windows.microsoft.com/shop From baustin <@t> cbgbiotech.com Fri Dec 25 12:31:04 2009 From: baustin <@t> cbgbiotech.com (Baustin) Date: Fri Dec 25 12:31:10 2009 Subject: [Histonet] Auto Reply Message-ID: <999910370@mail-server.cbgbiotech.com> Please do not respond to this automatic e-mail reply. I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies@cbgbiotech.com or fax it to 614-863-1676 Attn Ordering. If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225. Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG. From rsrichmond <@t> gmail.com Fri Dec 25 22:40:26 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Dec 25 22:40:29 2009 Subject: [Histonet] mishap with Carnoy's fixative Message-ID: I recently had a mishap with Carnoy's fixative that bears retelling on Histonet. I was preparing a colon cancer resection specimen - in a pathology service I've just started doing some work for - and needed a disclosing fixative for the mesenteric lymph nodes. What they had was Carnoy's fixative (6 parts alcohol, 3 parts chloroform, 1 part glacial acetic acid - I checked the MSDS and the chloroform was actually present). This was one of those labs that isn't allowed to have extra specimen containers on hand (a rather common problem in my experience - I think it's Good Management), so I was offered a plastic mammographic specimen container that happened to be lying around. I cut up the mesenteries into small pieces - no obviously positive lymph nodes - and left them in the Carnoy's fixative in the specimen container. The next day the plastic container had dissolved into a gooey mess that was intimately mixed with the fatty mesentery. It took two of us an hour to pick the stuff out. The lymph nodes were easy to find, though how the blocks will cut I don't know. The goo was somewhat soluble in xylene, so we used that. With inadequate ventilation, I stood there in a cramped position breathing in chloroform and xylene while I retrieved the lymph nodes. Chloroform has serious toxic problems and has no place in a hospital histology lab. If you have to use Carnoy's fixative, be very careful what you put it in. Bob Richmond Samurai Pathologist Knoxville TN From baustin <@t> cbgbiotech.com Sat Dec 26 12:04:40 2009 From: baustin <@t> cbgbiotech.com (Baustin) Date: Sat Dec 26 12:04:44 2009 Subject: [Histonet] Auto Reply Message-ID: <999975060@mail-server.cbgbiotech.com> Please do not respond to this automatic e-mail reply. I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies@cbgbiotech.com or fax it to 614-863-1676 Attn Ordering. If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225. Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG. From becky.garrison <@t> jax.ufl.edu Sat Dec 26 13:29:29 2009 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Sat Dec 26 13:29:34 2009 Subject: [Histonet] Hello fellow Histo's , 2 questions for you In-Reply-To: References: Message-ID: Here, we have either a pathologist or histotech take a cart with dissecting microscope to the kidney biopsy in Radiology and give the nephrologist who performs the procedure immediate feed back on whether the biopsy contains sufficient glomeruli. 95% of the time, the techs attend and the pathologist backs up when no tech is available. (This applies to native kidney biopsies only; transplant kidney biopsies are brought immediately to Pathology Gross Room by nephrology fellow or resident.) It is interesting that we have never charged for this 'immediate evaluation' service, neither technical nor professional. Are others who perform this service charging, and if so, what CPT code / modifier is used? We do not do frozens off site. Becky Garrison Pathology Supervisor Shands Jacksonville 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Thursday, December 24, 2009 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hello fellow Histo's , 2 questions for you First of all I hope all of you are enjoying your Holiday. And I expect that many of you are not at work like me, but when you get a chance if you could help me out by answering these two questions I would be very grateful. :-) 1) In your lab when you receive renal biopsy's fresh. Who finds the Glomeruli using a microscope? A pathologist or a Histologist? 2) Also, if you have frozen sections off site, do you always send a Histologist to assist the Pathologist? Thanks for your help in advance Many Blessing to each of you! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From baustin <@t> cbgbiotech.com Sun Dec 27 12:07:28 2009 From: baustin <@t> cbgbiotech.com (Baustin) Date: Sun Dec 27 12:07:32 2009 Subject: [Histonet] Auto Reply Message-ID: <1000040686@mail-server.cbgbiotech.com> Please do not respond to this automatic e-mail reply. I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies@cbgbiotech.com or fax it to 614-863-1676 Attn Ordering. If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225. Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG. From rsrichmond <@t> gmail.com Sun Dec 27 12:54:00 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sun Dec 27 12:54:04 2009 Subject: [Histonet] Re: gross evaluation of kidney biopsy specimens Message-ID: Becky Garrison (where?) asks: > Here, we have either a pathologist or histotech take a cart with > dissecting microscope to the kidney biopsy in Radiology and give the > nephrologist who performs the procedure immediate feed back on whether > the biopsy contains sufficient glomeruli. 95% of the time, the techs > attend and the pathologist backs up when no tech is available. (This > applies to native kidney biopsies only; transplant kidney biopsies are > brought immediately to Pathology Gross Room by nephrology fellow or > resident.) It is interesting that we have never charged for this 'immediate > evaluation' service, neither technical nor professional. Are others > who perform this service charging, and if so, what CPT code / modifier > is used? If I (a pathologist) did the evaluation, I'd charge 88329, pathology consultation during surgery, like I would for opening a colon resection specimen in the surgical suite but not doing a frozen section. I don't know whether this code is applicable when a histotech does the evaluation. Ouch! I hate to see a microscope subjected to the jolting and vibration it gets from being transported in a cart. I'd rather have the specimen brought to the laboratory. I like this procedure, because it allows the pathologist to have a dissecting microscope (stereo microscope). As I've mentioned before, I like to look at small bowel biopsy specimens done for suspected celiac disease, before I submit them for processing. Bob Richmond Samurai Pathologist Knoxville TN From mpence <@t> grhs.net Mon Dec 28 08:13:09 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Dec 28 08:13:13 2009 Subject: [Histonet] Hello fellow Histo's , 2 questions for you In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3CE9@is-e2k3.grhs.net> I don't know of a charge for immediate evaluation' services by technical staff? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garrison, Becky Sent: Saturday, December 26, 2009 1:29 PM To: Kim.Donadio@bhcpns.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Hello fellow Histo's , 2 questions for you Here, we have either a pathologist or histotech take a cart with dissecting microscope to the kidney biopsy in Radiology and give the nephrologist who performs the procedure immediate feed back on whether the biopsy contains sufficient glomeruli. 95% of the time, the techs attend and the pathologist backs up when no tech is available. (This applies to native kidney biopsies only; transplant kidney biopsies are brought immediately to Pathology Gross Room by nephrology fellow or resident.) It is interesting that we have never charged for this 'immediate evaluation' service, neither technical nor professional. Are others who perform this service charging, and if so, what CPT code / modifier is used? We do not do frozens off site. Becky Garrison Pathology Supervisor Shands Jacksonville 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org Sent: Thursday, December 24, 2009 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hello fellow Histo's , 2 questions for you First of all I hope all of you are enjoying your Holiday. And I expect that many of you are not at work like me, but when you get a chance if you could help me out by answering these two questions I would be very grateful. :-) 1) In your lab when you receive renal biopsy's fresh. Who finds the Glomeruli using a microscope? A pathologist or a Histologist? 2) Also, if you have frozen sections off site, do you always send a Histologist to assist the Pathologist? Thanks for your help in advance Many Blessing to each of you! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Mon Dec 28 08:20:39 2009 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Mon Dec 28 08:20:45 2009 Subject: [Histonet] Happy Holidays In-Reply-To: <000801ca8342$a2092930$e61b7b90$@callis@bresnan.net> References: <000801ca8342$a2092930$e61b7b90$@callis@bresnan.net> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A5522@EXCHMBC2.ad.ah.local> Thanks Gayle, same to you and to everyone. Jan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Tuesday, December 22, 2009 2:09 PM To: 'Histonet' Subject: [Histonet] Happy Holidays To all my friends and colleagues on Histonet I wish you a very Happy Holiday season filled with family and friends Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From akemiat3377 <@t> yahoo.com Mon Dec 28 11:51:34 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Mon Dec 28 11:52:27 2009 Subject: [Histonet] New Year Message-ID: <2EEDB89E-8B8A-4B56-9BFE-09F76ED5C899@yahoo.com> Good Morning Histoland, It's getting close to the end of the year. It has been very difficult for many of us, but I like this time of year. Not just because I get to spend time with family and friends, but because we get a fresh start. When the clock hits midnight on January 1, a new year begins. It's an optimistic time of year because we set up new goals, and we try to reach for the stars ? ? ? ? ? ? ? ? ? Best Wishes and Happy New Year, Akemi Akemi Allison BS, HT (ASCP) HTL From melissa.ribeiro <@t> brinegroup.com Mon Dec 28 12:03:51 2009 From: melissa.ribeiro <@t> brinegroup.com (Melissa Ribeiro) Date: Mon Dec 28 12:04:01 2009 Subject: [Histonet] Histology Manager opportunity - Connecticut Message-ID: <43904A2EECEAB54D8A023931049FEA4C1E9999@brin-sbs01.brinegroup.local> HISTOLOGY MANAGER - Connecticut Setting: Clinical, hospital-based lab Location: Connecticut The ideal candidate will have strong leadership skills, a vision of future technologies for Histology, ability to interact with our pathologists to keep the section on the "cutting edge", the ability to institute change within the section and get "buy in" from the staff. Great benefits package with competitive salary structure. Relocation assistance provided for out-of-state candidates. REQUIREMENTS: - Solid Histology background including IHC. - Supervisory/managerial experience required. - HT or HTL (ASCP) certification - Bachelor's degree in related field CONTACT: Melissa Ribeiro mribeiro@brinegroup.com (781) 272-3400 ext. 228 Melissa Ribeiro Healthcare Division Brine Group Staffing Solutions 20 Mall Road, Suite 225 Burlington, MA 01803 mribeiro@brinegroup.com Ph.? (781) 272-3400 ext. 228 Fax (781) 494-3401 Save a Tree! Consider the environment before you print this e-mail.? Visit our newly redesigned web site at www.brinegroup.com, the intelligent choice for staffing solutions. ? ? This electronic message contains information from Brine Group Staffing Solutions that may be privileged and confidential. The information is intended for the use of the addressee(s) only. If you are not an addressee, or received this email in error, note that any disclosure, copying, distribution, or use of the contents of this message is prohibited. If you have received this E-mail in error, please contact the sender immediately. From baustin <@t> cbgbiotech.com Mon Dec 28 12:11:20 2009 From: baustin <@t> cbgbiotech.com (Baustin) Date: Mon Dec 28 12:11:24 2009 Subject: [Histonet] Auto Reply Message-ID: <1000106346@mail-server.cbgbiotech.com> Please do not respond to this automatic e-mail reply. I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies@cbgbiotech.com or fax it to 614-863-1676 Attn Ordering. If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225. Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG. From Timothy.Morken <@t> ucsfmedctr.org Mon Dec 28 12:44:18 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Mon Dec 28 12:46:19 2009 Subject: [Histonet] Hello fellow Histo's , 2 questions for you In-Reply-To: References: Message-ID: <1AAF670737F193429070841C6B2ADD4C01214F0CFD@EXMBMCB15.ucsfmedicalcenter.org> Kim, for 11 years I attended all kidney bx's and determined their adequacy. It's not too hard, though you do have to take responsibility for asking for another stab if what they have gotten is no good, or too little. Tim Morken UCSF ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim.Donadio@bhcpns.org [Kim.Donadio@bhcpns.org] Sent: Thursday, December 24, 2009 7:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hello fellow Histo's , 2 questions for you First of all I hope all of you are enjoying your Holiday. And I expect that many of you are not at work like me, but when you get a chance if you could help me out by answering these two questions I would be very grateful. :-) 1) In your lab when you receive renal biopsy's fresh. Who finds the Glomeruli using a microscope? A pathologist or a Histologist? 2) Also, if you have frozen sections off site, do you always send a Histologist to assist the Pathologist? Thanks for your help in advance Many Blessing to each of you! Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jseaton <@t> wlgore.com Mon Dec 28 17:04:30 2009 From: jseaton <@t> wlgore.com (Janella Seaton) Date: Mon Dec 28 17:04:44 2009 Subject: [Histonet] Janella Seaton/WLGORE is out of the office. Message-ID: I will be out of the office starting 12/28/2009 and will not return until 01/05/2010. I will respond to your message when I return. Any histology-related requests or questions can be sent to: histology@wlgore.com. From rosenfeldtek <@t> hotmail.com Mon Dec 28 18:53:06 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Mon Dec 28 18:53:10 2009 Subject: [Histonet] Stain to differentiate Hemoglobin from Hemosiderin? Message-ID: I've seen Prussian blue as a stain for hemosiderin but that would also stain hemoglobin. Any ideas? Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Hotmail: Trusted email with Microsoft?s powerful SPAM protection. http://clk.atdmt.com/GBL/go/177141664/direct/01/ From raj <@t> bluemarble.net Mon Dec 28 19:11:58 2009 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Mon Dec 28 19:12:04 2009 Subject: [Histonet] Derm Lab Message-ID: <810E2E492AF64FA7A5DE46E2615CD35D@CHURCH> Any derm techs out there with some tips on starting a derm lab. Any information would be appreciated. Thanks raj From alyssa <@t> alliedsearchpartners.com Tue Dec 29 09:38:37 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Dec 29 09:38:43 2009 Subject: [Histonet] Histology/Dermato/Cytopathology Jobs Message-ID: Hello Everyone, I hope you all are enjoying the holidays. I wanted to inform everyone of a few openings that I am working on to see if there is anyone out there who would be interested. These are all full time and very permanent positions. Please send an updated resume to alyssa@alliedsearchpartners.com and myself or one of our recruiters will give you a call for an initial phone screen. Please be aware that all inquiries are kept confidential. Positions: Histotechnologist/Histotechnician 1. Plant City, FL 2. Schenectady, NY 3. Chicago, IL Positions: Dermatopathologist 1. Phoenix, AZ Positions: Cytopathology Fellowship 1. Phoenix, AZ -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From mcauliff <@t> umdnj.edu Tue Dec 29 10:24:27 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Dec 29 10:22:28 2009 Subject: [Histonet] Stain to differentiate Hemoglobin from Hemosiderin? In-Reply-To: References: Message-ID: <4B3A2D3B.7070204@umdnj.edu> No in my experience. Hemoglobin has iron but not in the same form or concentration as hemosiderin.Try it! Geoff JR R wrote: > I've seen Prussian blue as a stain for hemosiderin but that would also stain hemoglobin. > > Any ideas? > > Thanks, > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > _________________________________________________________________ > Hotmail: Trusted email with Microsoft?s powerful SPAM protection. > http://clk.atdmt.com/GBL/go/177141664/direct/01/_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From wlecorch <@t> rwjuhh.edu Tue Dec 29 10:59:55 2009 From: wlecorch <@t> rwjuhh.edu (Lecorchick, William) Date: Tue Dec 29 11:00:02 2009 Subject: [Histonet] RE: Vol 73, Issue 34 Processing Of CSF Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71A4AB78ACA@HAMEXMBA.rwjham.local> You may want to experiment with "NuView" from QC science (I have no ties or stand to gain anything from this) it will yield a result similar to a Sure path or ThinPrep without the equipment and expense (assuming your volume or budget could not support either system) From baustin <@t> cbgbiotech.com Tue Dec 29 12:06:33 2009 From: baustin <@t> cbgbiotech.com (Baustin) Date: Tue Dec 29 12:06:41 2009 Subject: [Histonet] Auto Reply Message-ID: <1000171728@mail-server.cbgbiotech.com> Please do not respond to this automatic e-mail reply. I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies@cbgbiotech.com or fax it to 614-863-1676 Attn Ordering. If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225. Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG. From erweber <@t> maxhealth.com Tue Dec 29 13:53:37 2009 From: erweber <@t> maxhealth.com (Eric Weber) Date: Tue Dec 29 13:53:45 2009 Subject: [Histonet] Part Time MOHS Tech Needed in Portland Message-ID: <9D12D4EF30176D4F839EB47F3D0E843611FAAF83@exbk2.maxhealth.com> MOHs Tech needed at the Portland VAMC for a Part Time Basis. Please contact me if you would be interested. Details: Dermatology MOHS Health Technician The Portland VA Medical Center is in need of one part-time Dermatology MOHS technician for the Dermatology Clinic, Operative Care Program at the Portland VA Medical Center, 3710 SW US Veterans Hospital Road, Portland, OR 97239. Qualifications: Knowledge of basic methods and procedures of the Dermatology clinic. Candidate shall demonstrate skill and precision in use of the tools, materials and equipment for Dermatology clinic. Knowledge of specialized terminology used in the specialized area of the facility where the work is performed. Strong customer service skills; detail oriented; and adaptability to work in a fast paced work environment. Candidate will serve as technical expert in the operation and maintenance of specialized equipment and instruments. Experience: Basic computer knowledge. Candidate shall demonstrate experience and skill in cutting and preparing frozen sections of tissue in a Dermatology MOHS clinic. Familiarity with medical terminology and familiarity with routine laboratory values and abbreviations are required. Specialty Experience (a Plus but not required): Familiarity with the VA electronic medical record system (CPRS). Length of Assignment: One year, or longer. On-Call Hours: Not available Overtime: Not available Tour of Duty: Mondays for 8 hours, (7:30am to 3:30pm), Fridays for 5 hours (7:30 am to 12:30 pm). Eric Weber Maxim Government Services 7227 Lee DeForest Dr Columbia, MD 21046 phone: (410) 910-4942 toll free: (866) 260-9142 fax:(410) 953-8358 erweber@maxhealth.com CONFIDENTIALITY NOTE: This e-mail message contains confidential, privileged or otherwise protected information intended solely for the addressee. Please do not read, copy, or disseminate it unless you are the intended addressee. If you have received it in error, please call us (collect) at (410) 910-1500 and ask to speak with the message sender. Also, we would appreciate your forwarding the message back to us and deleting it from your system. Thank you. This e-mail and all other electronic (including voice) communications from the sender's firm are for informational purposes only. No such communication is intended by the sender to constitute either an electronic record or an electronic signature or to constitute any agreement by the sender to conduct a transaction by electronic means. Any such intention or agreement is hereby expressly disclaimed unless otherwise specifically indicated. From rgrow <@t> bmnet.com Tue Dec 29 15:04:22 2009 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Tue Dec 29 15:04:24 2009 Subject: [Histonet] Re: Recycling Formalin, Xylene, and Alcohol In-Reply-To: Message-ID: Andria, CBG is very reliable. I have one of the large capacity units called: Solv Solv. It's set up to run xylene on one side and alcohol on the other. Very convenient, no flushes to do. I don't recommend recycling formalin, to much fume exposure, testing, rebuffering, etc., but this unit will work for formalin too. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax You wrote: Message: 4 Date: Mon, 21 Dec 2009 14:19:36 -0500 From: "Evans, Andria B" Subject: [Histonet] Recycling Formalin, Xylene, and Alcohol To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I would like to know what everyone out in histoland is using to recycle there solutions. We are looking at switching out the procyclers (that are about 10 years old) to something else. Any Pros/Cons of current methods would be great. Thanks!! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 From ruebenjcarter <@t> gmail.com Tue Dec 29 16:16:04 2009 From: ruebenjcarter <@t> gmail.com (R C) Date: Tue Dec 29 16:16:08 2009 Subject: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome Message-ID: <2a926e3f0912291416u723bd5d4ya6b93e199b4a3d0@mail.gmail.com> Hi. I'm working on a protocol for cutting monkey brain sections to be mounted on 2x3 slides. I've read about utilizing a sliding microtome but in short, have decided to use the 2x3 adapter for a standard Microm microtome. During microtomy I've noticed many wrinkles in the sections, particularly within the folds of the cerebellum. The wrinkles worsen as the sections float in the water bath (temp=38). In troubleshooting, a co-worker suggests inadequate fixation. I on the other hand believe that the wrinkles relate to the Type R paraffin, which contains polymers as well as the use of the adapter versus the sliding microtome. Can anyone offer any first hand experience/guidance? Thanks From rsrichmond <@t> gmail.com Tue Dec 29 16:26:22 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Tue Dec 29 16:26:26 2009 Subject: [Histonet] Re: Stain to differentiate Hemoglobin from Hemosiderin? Message-ID: Jerry Ricks, Research Scientist, University of Washington,Department of Pathology asks: >>I've seen Prussian blue as a stain for hemosiderin but that would also stain hemoglobin.<< The Perls prussian blue reaction occurs with hemosiderin ("stainable iron") but not with hemoglobin, hematin, formalin pigment, malarial pigment, or melanin. It isn't a stain, but a reaction between ferric iron and ferrocyanide ion that produces a dense blue precipitate. The Stainsfile page doesn't really answer your questions, and I'd suggest looking it up in some of the standard textbooks. Bob Richmond Samurai Pathologist Knoxville TN From lucy.zong <@t> gmail.com Tue Dec 29 17:05:10 2009 From: lucy.zong <@t> gmail.com (Lucy Zong) Date: Tue Dec 29 17:05:16 2009 Subject: [Histonet] LEICA 2055 AUTOCUT Message-ID: <8daef62e0912291505l7721b8acw2c339e97a29ddb6f@mail.gmail.com> I was given a Leica 2055 microtome for our lab, however it did not come with an operators manual. Does anyone have one they could e-mail to me? Thank you From jkiernan <@t> uwo.ca Tue Dec 29 19:37:52 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Dec 29 19:37:56 2009 Subject: [Histonet] Stain to differentiate Hemoglobin from Hemosiderin? Message-ID: Please explain. The iron in haemoglobin is tightly protein-bound and not stainable by histochemical methods for the iron in haemosiderin and ferritin. Red blood cells are, for example, Prussian-blue negative. John Kiernan UWO. London, Canada == == == ----- Original Message ----- From: JR R Date: Monday, December 28, 2009 19:54 Subject: [Histonet] Stain to differentiate Hemoglobin from Hemosiderin? To: histonet@lists.utsouthwestern.edu > > I've seen Prussian blue as a stain for hemosiderin but that > would also stain hemoglobin. > > Any ideas? > > Thanks, > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > _________________________________________________________________ > Hotmail: Trusted email with Microsoft?s powerful SPAM protection. > http://clk.atdmt.com/GBL/go/177141664/direct/01/_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From birnbaumm <@t> asaf.health.gov.il Wed Dec 30 04:17:15 2009 From: birnbaumm <@t> asaf.health.gov.il (birnbaumm@asaf.health.gov.il) Date: Wed Dec 30 04:18:14 2009 Subject: [Histonet] Probe for mouse Y chromosome for FISH Message-ID: <054452CCC076BE4DA21E46AE95E32EC3277BE9@mail2.asaf.health.gov.il> Dear all We look for Fluorescence probe of mouse Y chromosome. Which company does sale it? Mira Birnbaum Pathology Asaf Hrofeh Medical Center Israel From cdemarinis <@t> SARATOGACARE.ORG Wed Dec 30 07:17:46 2009 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis, Carolyn) Date: Wed Dec 30 07:17:53 2009 Subject: [Histonet] FNA code Message-ID: Which CPT code are labs using for fine needle aspirations that are processed using thinprep technique - FNA interpretation and report-88173 or thinprep non-gyn 88112? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. From esulkosky <@t> gmail.com Wed Dec 30 08:40:09 2009 From: esulkosky <@t> gmail.com (Eric Sulkosky) Date: Wed Dec 30 08:40:15 2009 Subject: [Histonet] Derm Lab Message-ID: <6c3840890912300640p70132627w9befa4fcca15117f@mail.gmail.com> RAJ, What tips are you looking for? Are you starting from scratch with an empty room or has the room already been equipped with ventilation, plumbing and electrical? Eric From DKBoyd <@t> chs.net Wed Dec 30 08:42:03 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Wed Dec 30 08:41:55 2009 Subject: [Histonet] FNA code In-Reply-To: Message-ID: 88173 Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Demarinis, Carolyn" Sent by: histonet-bounces@lists.utsouthwestern.edu 12/30/2009 08:18 AM To cc Subject [Histonet] FNA code Which CPT code are labs using for fine needle aspirations that are processed using thinprep technique - FNA interpretation and report-88173 or thinprep non-gyn 88112? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From xipamanine <@t> gmail.com Wed Dec 30 09:37:58 2009 From: xipamanine <@t> gmail.com (Xipamanine Mkuze) Date: Wed Dec 30 09:38:03 2009 Subject: [Histonet] Probe for mouse Y chromosome for FISH In-Reply-To: <054452CCC076BE4DA21E46AE95E32EC3277BE9@mail2.asaf.health.gov.il> References: <054452CCC076BE4DA21E46AE95E32EC3277BE9@mail2.asaf.health.gov.il> Message-ID: <3cfdeb590912300737g155fbd26v1e2a7b0ed98d387d@mail.gmail.com> Cambio (http://www.cambio.co.uk) 2009/12/30 > Dear all > > We look for Fluorescence probe of mouse Y chromosome. Which company does > sale it? > > Mira Birnbaum > > Pathology > > Asaf Hrofeh Medical Center > > Israel > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sfeher <@t> CMC-NH.ORG Wed Dec 30 10:43:31 2009 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Wed Dec 30 10:43:36 2009 Subject: [Histonet] LEICA 2055 AUTOCUT In-Reply-To: <8daef62e0912291505l7721b8acw2c339e97a29ddb6f@mail.gmail.com> References: <8daef62e0912291505l7721b8acw2c339e97a29ddb6f@mail.gmail.com> Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B74914@exchange.cmc-nh.org> I have one for the RM2255 if you think that will help. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucy Zong Sent: Tuesday, December 29, 2009 6:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LEICA 2055 AUTOCUT I was given a Leica 2055 microtome for our lab, however it did not come with an operators manual. Does anyone have one they could e-mail to me? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jsantiago <@t> bellsouth.net Wed Dec 30 11:08:15 2009 From: jsantiago <@t> bellsouth.net (Jerry Santiago) Date: Wed Dec 30 11:09:49 2009 Subject: [Histonet] Mississippi Histotechnology Homecoming Message-ID: <861067.47883.qm@web180409.mail.gq1.yahoo.com> Mississippi Histotechnology Homecoming Calling all Histotechnologists from the State of Mississippi. The awaited homecoming for the Mississippi Society of Histotechnology is finally here.? The meeting information will be available in January 2010. To receive the information, please send an e-mail with name and address to my attention to: jsantiago@bellsouth.net. Event: Mississippi Histotechnology Homecoming Event Dates: March 12 ? 14, 2010 Site: The Beau Rivage Resort & Casino, Biloxi, Mississippi ? 7 (21 ceu?s) Workshops to include Supv & Administration, Routine Histology/Special Stains, & Immunohistochemistry. ? Plan for this great event and the opportunity to reorganize the state society. ? Sincerely, ? Jerry Santiago, BS, HTL(ASCP)QIHC NSH Region III Director From azdudley <@t> hotmail.com Wed Dec 30 12:03:36 2009 From: azdudley <@t> hotmail.com (anita dudley) Date: Wed Dec 30 12:03:40 2009 Subject: [Histonet] cap question amp.12087 Message-ID: just wondering if everyone is defrosting their cryostats every week? seems like overkill, we clean ours out daily and every week clean with 100 alc. what are others doing? this is a revised question. thanks a lot anita dudley providence hospital mobile _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/171222985/direct/01/ From gu.lang <@t> gmx.at Wed Dec 30 12:18:54 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Dec 30 12:19:01 2009 Subject: AW: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome In-Reply-To: <2a926e3f0912291416u723bd5d4ya6b93e199b4a3d0@mail.gmail.com> References: <2a926e3f0912291416u723bd5d4ya6b93e199b4a3d0@mail.gmail.com> Message-ID: <393235095CC64FF18D6F370D21E71EEA@dielangs.at> Have you tried to float the section first in a roomtemp. waterbath (bowl with tapwater)? There you can flatten the wrinkles with a brush, then mount it on the glass slide and bring it into the warm water. While gliding from the glass you can also use the brush to pull softly on the section to stretch it. If the infiltrated tissue is still too soft, I suggest longer infiltration time in xylen and paraffin to get rid of the fatty part of brain tissue. Sometimes even cutting on next day of embedding (one day waiting) helps. Do you cool the blocks before cutting? Long enough? For big blocks we usually use one blade for trimming and a new one for getting a thin section. Hope this helps Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von R C Gesendet: Dienstag, 29. Dezember 2009 23:16 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome Hi. I'm working on a protocol for cutting monkey brain sections to be mounted on 2x3 slides. I've read about utilizing a sliding microtome but in short, have decided to use the 2x3 adapter for a standard Microm microtome. During microtomy I've noticed many wrinkles in the sections, particularly within the folds of the cerebellum. The wrinkles worsen as the sections float in the water bath (temp=38). In troubleshooting, a co-worker suggests inadequate fixation. I on the other hand believe that the wrinkles relate to the Type R paraffin, which contains polymers as well as the use of the adapter versus the sliding microtome. Can anyone offer any first hand experience/guidance? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From baustin <@t> cbgbiotech.com Wed Dec 30 12:29:09 2009 From: baustin <@t> cbgbiotech.com (Baustin) Date: Wed Dec 30 12:29:12 2009 Subject: [Histonet] Auto Reply Message-ID: <1000237988@mail-server.cbgbiotech.com> Please do not respond to this automatic e-mail reply. I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies@cbgbiotech.com or fax it to 614-863-1676 Attn Ordering. If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225. Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG. From jeri <@t> opssearchgroup.com Wed Dec 30 12:39:13 2009 From: jeri <@t> opssearchgroup.com (jeri@opssearchgroup.com) Date: Wed Dec 30 12:39:37 2009 Subject: [Histonet] Sr HTL opportunity in NC Message-ID: <87140935C4DA4270AE1356959FA8558E@D3RWK391> Now that 2009 is coming to a close, some of you might have already made a new year's resolution to find a more challenging HTL opportunity. If that is true, please note this great opportunity in a wonderful city in NC. The right candidate will take a lead role in the development and implementation of new procedures, instrumentation, computer functionality, etc. Assumes responsibility for one or more major on-going laboratory projects or functions or the equivalent - i.e. CQI, safety, instrumentation, quality control, regulatory, administrative, staff development, computer (LIS,HIS, hospital intranet, PC applications) etc. Serves as a resource in area of responsibility. Performs testing that requires specialized knowledge within a discipline and/or crosses disciplines. Trains new employees in the theoretical and operational aspects, procedures and evaluates their work. Acts as a resource for less experienced co-workers. Assists supervisor/manager in review of laboratory reports, procedures, quality control. 5 year experience in Histology Department to include immuno histochemistry. Bachelors Degree HT or HTL (ASCP) certification. I would be happy to share more details with anyone who might be interested. Jeri Vitello OPS Search Group 574.633.1231 www.opssearchgroup.com jeri@opssearchgroup.com where OPPORTUNITY and PEOPLE meet SUCCESSFULLY From vgrover <@t> polysciences.com Wed Dec 30 13:28:20 2009 From: vgrover <@t> polysciences.com (Valantou Grover) Date: Wed Dec 30 13:28:29 2009 Subject: [Histonet] RE: Histonet Digest, Vol 73, Issue 39, Question 10. Dermlab Message-ID: <2C12F4C64F364FCEBCAFBDC0068CA1E7@USWARD13ZFB71> Rebecca, There are many companies that will set up the lab from beginning to end, TBS, Inc. has this service. You can contact them at 919-384-9393. Valantou Grover, HT/HTL(ASCP), PA, MBA Biosciences Product Line Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 Fax: 1-800-343-3291 Phone number: 1-800-523-2575 X7418 Direct:1-215-488-7418 Cell phone: 1-215-409-8327 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 73, Issue 39 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Auto Reply (Baustin) 2. Part Time MOHS Tech Needed in Portland (Eric Weber) 3. Re: Recycling Formalin, Xylene, and Alcohol (rgrow@bmnet.com) 4. 2x3 microtomy: Adapter vs Sliding Microtome (R C) 5. Re: Stain to differentiate Hemoglobin from Hemosiderin? (Robert Richmond) 6. LEICA 2055 AUTOCUT (Lucy Zong) 7. Re: Stain to differentiate Hemoglobin from Hemosiderin? (John Kiernan) 8. Probe for mouse Y chromosome for FISH (birnbaumm@asaf.health.gov.il) 9. FNA code (Demarinis, Carolyn) 10. Derm Lab (Eric Sulkosky) 11. Re: FNA code (DKBoyd@chs.net) 12. Re: Probe for mouse Y chromosome for FISH (Xipamanine Mkuze) 13. RE: LEICA 2055 AUTOCUT (Feher, Stephen) 14. Mississippi Histotechnology Homecoming (Jerry Santiago) ---------------------------------------------------------------------- Message: 1 Date: Tue, 29 Dec 2009 13:06:33 -0500 From: Baustin Subject: [Histonet] Auto Reply To: Message-ID: <1000171728@mail-server.cbgbiotech.com> Content-Type: text/plain; charset="utf-8" Please do not respond to this automatic e-mail reply. I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies@cbgbiotech.com or fax it to 614-863-1676 Attn Ordering. If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225. Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG. ------------------------------ Message: 2 Date: Tue, 29 Dec 2009 14:53:37 -0500 From: Eric Weber Subject: [Histonet] Part Time MOHS Tech Needed in Portland To: Message-ID: <9D12D4EF30176D4F839EB47F3D0E843611FAAF83@exbk2.maxhealth.com> Content-Type: text/plain; charset="us-ascii" MOHs Tech needed at the Portland VAMC for a Part Time Basis. Please contact me if you would be interested. Details: Dermatology MOHS Health Technician The Portland VA Medical Center is in need of one part-time Dermatology MOHS technician for the Dermatology Clinic, Operative Care Program at the Portland VA Medical Center, 3710 SW US Veterans Hospital Road, Portland, OR 97239. Qualifications: Knowledge of basic methods and procedures of the Dermatology clinic. Candidate shall demonstrate skill and precision in use of the tools, materials and equipment for Dermatology clinic. Knowledge of specialized terminology used in the specialized area of the facility where the work is performed. Strong customer service skills; detail oriented; and adaptability to work in a fast paced work environment. Candidate will serve as technical expert in the operation and maintenance of specialized equipment and instruments. Experience: Basic computer knowledge. Candidate shall demonstrate experience and skill in cutting and preparing frozen sections of tissue in a Dermatology MOHS clinic. Familiarity with medical terminology and familiarity with routine laboratory values and abbreviations are required. Specialty Experience (a Plus but not required): Familiarity with the VA electronic medical record system (CPRS). Length of Assignment: One year, or longer. On-Call Hours: Not available Overtime: Not available Tour of Duty: Mondays for 8 hours, (7:30am to 3:30pm), Fridays for 5 hours (7:30 am to 12:30 pm). Eric Weber Maxim Government Services 7227 Lee DeForest Dr Columbia, MD 21046 phone: (410) 910-4942 toll free: (866) 260-9142 fax:(410) 953-8358 erweber@maxhealth.com CONFIDENTIALITY NOTE: This e-mail message contains confidential, privileged or otherwise protected information intended solely for the addressee. Please do not read, copy, or disseminate it unless you are the intended addressee. If you have received it in error, please call us (collect) at (410) 910-1500 and ask to speak with the message sender. Also, we would appreciate your forwarding the message back to us and deleting it from your system. Thank you. This e-mail and all other electronic (including voice) communications from the sender's firm are for informational purposes only. No such communication is intended by the sender to constitute either an electronic record or an electronic signature or to constitute any agreement by the sender to conduct a transaction by electronic means. Any such intention or agreement is hereby expressly disclaimed unless otherwise specifically indicated. ------------------------------ Message: 3 Date: Tue, 29 Dec 2009 16:04:22 -0500 From: rgrow@bmnet.com Subject: [Histonet] Re: Recycling Formalin, Xylene, and Alcohol To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Andria, CBG is very reliable. I have one of the large capacity units called: Solv Solv. It's set up to run xylene on one side and alcohol on the other. Very convenient, no flushes to do. I don't recommend recycling formalin, to much fume exposure, testing, rebuffering, etc., but this unit will work for formalin too. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax You wrote: Message: 4 Date: Mon, 21 Dec 2009 14:19:36 -0500 From: "Evans, Andria B" Subject: [Histonet] Recycling Formalin, Xylene, and Alcohol To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I would like to know what everyone out in histoland is using to recycle there solutions. We are looking at switching out the procyclers (that are about 10 years old) to something else. Any Pros/Cons of current methods would be great. Thanks!! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 ------------------------------ Message: 4 Date: Tue, 29 Dec 2009 14:16:04 -0800 From: R C Subject: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0912291416u723bd5d4ya6b93e199b4a3d0@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi. I'm working on a protocol for cutting monkey brain sections to be mounted on 2x3 slides. I've read about utilizing a sliding microtome but in short, have decided to use the 2x3 adapter for a standard Microm microtome. During microtomy I've noticed many wrinkles in the sections, particularly within the folds of the cerebellum. The wrinkles worsen as the sections float in the water bath (temp=38). In troubleshooting, a co-worker suggests inadequate fixation. I on the other hand believe that the wrinkles relate to the Type R paraffin, which contains polymers as well as the use of the adapter versus the sliding microtome. Can anyone offer any first hand experience/guidance? Thanks ------------------------------ Message: 5 Date: Tue, 29 Dec 2009 17:26:22 -0500 From: Robert Richmond Subject: [Histonet] Re: Stain to differentiate Hemoglobin from Hemosiderin? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Jerry Ricks, Research Scientist, University of Washington,Department of Pathology asks: >>I've seen Prussian blue as a stain for hemosiderin but that would also stain hemoglobin.<< The Perls prussian blue reaction occurs with hemosiderin ("stainable iron") but not with hemoglobin, hematin, formalin pigment, malarial pigment, or melanin. It isn't a stain, but a reaction between ferric iron and ferrocyanide ion that produces a dense blue precipitate. The Stainsfile page doesn't really answer your questions, and I'd suggest looking it up in some of the standard textbooks. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 6 Date: Tue, 29 Dec 2009 18:05:10 -0500 From: Lucy Zong Subject: [Histonet] LEICA 2055 AUTOCUT To: histonet@lists.utsouthwestern.edu Message-ID: <8daef62e0912291505l7721b8acw2c339e97a29ddb6f@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I was given a Leica 2055 microtome for our lab, however it did not come with an operators manual. Does anyone have one they could e-mail to me? Thank you ------------------------------ Message: 7 Date: Tue, 29 Dec 2009 20:37:52 -0500 From: John Kiernan Subject: Re: [Histonet] Stain to differentiate Hemoglobin from Hemosiderin? To: JR R Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=windows-1252 Please explain. The iron in haemoglobin is tightly protein-bound and not stainable by histochemical methods for the iron in haemosiderin and ferritin. Red blood cells are, for example, Prussian-blue negative. John Kiernan UWO. London, Canada == == == ----- Original Message ----- From: JR R Date: Monday, December 28, 2009 19:54 Subject: [Histonet] Stain to differentiate Hemoglobin from Hemosiderin? To: histonet@lists.utsouthwestern.edu > > I've seen Prussian blue as a stain for hemosiderin but that > would also stain hemoglobin. > > Any ideas? > > Thanks, > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > _________________________________________________________________ > Hotmail: Trusted email with Microsofts powerful SPAM protection. > http://clk.atdmt.com/GBL/go/177141664/direct/01/____________________________ ___________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 30 Dec 2009 12:17:15 +0200 From: Subject: [Histonet] Probe for mouse Y chromosome for FISH To: Message-ID: <054452CCC076BE4DA21E46AE95E32EC3277BE9@mail2.asaf.health.gov.il> Content-Type: text/plain; charset="windows-1255" Dear all We look for Fluorescence probe of mouse Y chromosome. Which company does sale it? Mira Birnbaum Pathology Asaf Hrofeh Medical Center Israel ------------------------------ Message: 9 Date: Wed, 30 Dec 2009 08:17:46 -0500 From: "Demarinis, Carolyn" Subject: [Histonet] FNA code To: Message-ID: Content-Type: text/plain; charset="us-ascii" Which CPT code are labs using for fine needle aspirations that are processed using thinprep technique - FNA interpretation and report-88173 or thinprep non-gyn 88112? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. ------------------------------ Message: 10 Date: Wed, 30 Dec 2009 09:40:09 -0500 From: Eric Sulkosky Subject: [Histonet] Derm Lab To: histonet@lists.utsouthwestern.edu Message-ID: <6c3840890912300640p70132627w9befa4fcca15117f@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 RAJ, What tips are you looking for? Are you starting from scratch with an empty room or has the room already been equipped with ventilation, plumbing and electrical? Eric ------------------------------ Message: 11 Date: Wed, 30 Dec 2009 09:42:03 -0500 From: DKBoyd@chs.net Subject: Re: [Histonet] FNA code To: "Demarinis, Carolyn" Cc: histonet-bounces@lists.utsouthwestern.edu, HISTONET@PATHOLOGY.SWMED.EDU Message-ID: Content-Type: text/plain; charset="US-ASCII" 88173 Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Demarinis, Carolyn" Sent by: histonet-bounces@lists.utsouthwestern.edu 12/30/2009 08:18 AM To cc Subject [Histonet] FNA code Which CPT code are labs using for fine needle aspirations that are processed using thinprep technique - FNA interpretation and report-88173 or thinprep non-gyn 88112? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 12 Date: Wed, 30 Dec 2009 16:37:58 +0100 From: Xipamanine Mkuze Subject: Re: [Histonet] Probe for mouse Y chromosome for FISH To: birnbaumm@asaf.health.gov.il Cc: histonet@lists.utsouthwestern.edu Message-ID: <3cfdeb590912300737g155fbd26v1e2a7b0ed98d387d@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Cambio (http://www.cambio.co.uk) 2009/12/30 > Dear all > > We look for Fluorescence probe of mouse Y chromosome. Which company does > sale it? > > Mira Birnbaum > > Pathology > > Asaf Hrofeh Medical Center > > Israel > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Wed, 30 Dec 2009 11:43:31 -0500 From: "Feher, Stephen" Subject: RE: [Histonet] LEICA 2055 AUTOCUT To: "Lucy Zong" , Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B74914@exchange.cmc-nh.org> Content-Type: text/plain; charset="us-ascii" I have one for the RM2255 if you think that will help. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucy Zong Sent: Tuesday, December 29, 2009 6:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LEICA 2055 AUTOCUT I was given a Leica 2055 microtome for our lab, however it did not come with an operators manual. Does anyone have one they could e-mail to me? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 30 Dec 2009 09:08:15 -0800 (PST) From: Jerry Santiago Subject: [Histonet] Mississippi Histotechnology Homecoming To: histonet@lists.utsouthwestern.edu Message-ID: <861067.47883.qm@web180409.mail.gq1.yahoo.com> Content-Type: text/plain; charset=utf-8 Mississippi Histotechnology Homecoming Calling all Histotechnologists from the State of Mississippi. The awaited homecoming for the Mississippi Society of Histotechnology is finally here.B The meeting information will be available in January 2010. To receive the information, please send an e-mail with name and address to my attention to: jsantiago@bellsouth.net. Event: Mississippi Histotechnology Homecoming Event Dates: March 12 b From rgrow <@t> bmnet.com Wed Dec 30 13:51:42 2009 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Wed Dec 30 13:51:45 2009 Subject: [Histonet] Re: Histonet Digest, Vol 73, Issue 39 In-Reply-To: Message-ID: R C, In past years, I've section many primate brains in normal size sections but not whole, so this may/may not help. If it was fixation you would not be able to get a complete section in the first place, so your fixation should not be a problem. If it was the adaptor on your microtome you would have other sectioning problems: wobble, thick/thin, washboard, curved sections, uneven thickness, etc. A couple of suggestions that might help. The suggestions should work regardless of section thickness. 1. Try initially laying your ribbon on a dish of COLD water. With fine forceps, (chilled too) gently stretch out your section. When you are satisfied pick your section up from the cold and transfer it to the warm water to complete the process. Lengthens your cutting time, but works well if you have the time. 2. Be sure to use good qualilty high profile blades, if your stage/faceplate will accomodate them. Surgipath has good ones. For large sections they are more stable. 3. I did use the Surgipath Infiltration Medium for the processor. And, yes, it does make a difference. Contact your Surgipath Sales Rep and request "Infiltration Medium" to set up in your processor. You might be able to get enough for a trial run. Continue to use your "Type R" for embedding. You may need a combination of these things to get the quality you are looking for. Let me know if I can help further. Happy New Year to ALL Histonetters! Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax You wrote: Message: 4 Date: Tue, 29 Dec 2009 14:16:04 -0800 From: R C Subject: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0912291416u723bd5d4ya6b93e199b4a3d0@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi. I'm working on a protocol for cutting monkey brain sections to be mounted on 2x3 slides. I've read about utilizing a sliding microtome but in short, have decided to use the 2x3 adapter for a standard Microm microtome. During microtomy I've noticed many wrinkles in the sections, particularly within the folds of the cerebellum. The wrinkles worsen as the sections float in the water bath (temp=38). In troubleshooting, a co-worker suggests inadequate fixation. I on the other hand believe that the wrinkles relate to the Type R paraffin, which contains polymers as well as the use of the adapter versus the sliding microtome. Can anyone offer any first hand experience/guidance? From alyssa <@t> alliedsearchpartners.com Wed Dec 30 14:20:35 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Wed Dec 30 14:20:39 2009 Subject: [Histonet] Job In Northern NY Message-ID: Allied Search Partners is currently accepting resumes for qualified histotechnician/histotechnologist applicants for a laboratory just 30 miles north of Schenectady, NY. Job Description: Histotechnologists/Histotechnicians Shifts: 11am-7pm, Monday-Friday, Full Time. Benefits: Great benefit package included with offer Please submit your resume for prescreening purposes to alyssa@alliedsearchpartners.com Sign On Bonus: Available through Allied Search Partners *All inquiries are always kept confidential* upon resume submission one of our recruiters will call you for an initial phone interview. No resume will be submitted before an initial phone interview. Be sure to visit our website www.alliedsearchpartners.com to submit your job search request, refer a friend for $$Cash Bonus$$, and have your resume reviewed by our career advisors. -- Here's to a 2010 that EXCEEDS expectations! Alyssa Peterson Director of Recruitment O: 888.388.7571 x 102 F: 888.388.7572 *Be sure to visit us on the web at www.alliedsearchpartners.com From sfeher <@t> CMC-NH.ORG Wed Dec 30 16:16:36 2009 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Wed Dec 30 16:16:40 2009 Subject: [Histonet] Path Specimen Source Files Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B74922@exchange.cmc-nh.org> I am interested to know if any of you out there might have a fairly comprehensive set of Pathology Specimen source files in either Word or Excel. We are in the process of training our Registration personnel to be able to register and accession Pathology specimens. By increasing our source files to make it more comprehensive, we should be able to make it an easier, and more precise, task to accession a variety of pathology specimens into our SoftPath system. The source files that came with the system are general in nature so that we can add to them based on a general root system (i.e. Respiratory, Urinary Tract, etc.). Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org From princekunlzy <@t> gmail.com Wed Dec 30 18:56:49 2009 From: princekunlzy <@t> gmail.com (Adeluwoye Oluwatosin) Date: Wed Dec 30 18:56:53 2009 Subject: [Histonet] Formalin pigment? Message-ID: <786f96610912301656o71e21b25vae62e9ff644de4ed@mail.gmail.com> Hello, please i am working effects of various concentration of formaldehyde on liver tissue. The sections shows some degree of formalin pigmentation form 20 to 35% and concentrated formaldehyde. Can anyone ofer advise as to how to quantittate o judge the varying degree of pigmentation? Tosin From annigyg <@t> gmail.com Thu Dec 31 02:52:21 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Dec 31 02:52:35 2009 Subject: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome In-Reply-To: <393235095CC64FF18D6F370D21E71EEA@dielangs.at> References: <2a926e3f0912291416u723bd5d4ya6b93e199b4a3d0@mail.gmail.com> <393235095CC64FF18D6F370D21E71EEA@dielangs.at> Message-ID: i spent many many years handling pig, mouse and human brain, cutting sections on 2x3 slides. the trick is to use the tap water float out before the warm water, also to cut sections thicker than normal - cut at 10-15 and maybe even 20 microns - trouble is at that thickness AND it being animal tissue, the sections wash off in a flash - unless you use adhesive and cook the sections on to the slides gently at 32C for at least overnight i have some 20micron silver stained cerebellum on 2x3 slides here with me right now, 20 years later they are still good as new!! good luck Annie 2009/12/30 Gudrun Lang > Have you tried to float the section first in a roomtemp. waterbath (bowl > with tapwater)? There you can flatten the wrinkles with a brush, then mount > it on the glass slide and bring it into the warm water. While gliding from > the glass you can also use the brush to pull softly on the section to > stretch it. > > If the infiltrated tissue is still too soft, I suggest longer infiltration > time in xylen and paraffin to get rid of the fatty part of brain tissue. > Sometimes even cutting on next day of embedding (one day waiting) helps. > Do you cool the blocks before cutting? Long enough? > > For big blocks we usually use one blade for trimming and a new one for > getting a thin section. > > Hope this helps > Gudrun > > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von R C > Gesendet: Dienstag, 29. Dezember 2009 23:16 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome > > Hi. I'm working on a protocol for cutting monkey brain sections to be > mounted on 2x3 slides. I've read about utilizing a sliding microtome but in > short, have decided to use the 2x3 adapter for a standard Microm microtome. > During microtomy I've noticed many wrinkles in the sections, particularly > within the folds of the cerebellum. The wrinkles worsen as the sections > float in the water bath (temp=38). > > In troubleshooting, a co-worker suggests inadequate fixation. I on the > other > hand believe that the wrinkles relate to the Type R paraffin, which > contains > polymers as well as the use of the adapter versus the sliding microtome. > > Can anyone offer any first hand experience/guidance? > > Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From Peter.Tree <@t> abdserotec.com Thu Dec 31 04:03:12 2009 From: Peter.Tree <@t> abdserotec.com (Peter Tree) Date: Thu Dec 31 04:12:54 2009 Subject: [Histonet] RE: Probe for mouse Y chromosome for FISH In-Reply-To: <054452CCC076BE4DA21E46AE95E32EC3277BE9@mail2.asaf.health.gov.il> References: <054452CCC076BE4DA21E46AE95E32EC3277BE9@mail2.asaf.health.gov.il> Message-ID: Hi Mira, Doubtless quite a few companies out there. Try Cambio: http://www.cambio.co.uk/catalogue-Star_FISH_copy_Mouse_Chromosome_Specific_Probes_Biotin_Labelled_br_Concentrated_Format-1-426 Conc Mouse WCP Biotin Chromosome Y Catalogue number 1187-YMB-02 Have a great 2010 Peter Peter Tree Account Manager Custom Antibody Generation AbD Serotec - Your first choice for antibodies! (A Division of MorphoSys) Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxon. OX5 1GE. Tel: +44 (0)1865 852700 Fax: +44 (0)1865 373899 Direct: +44 (0)1865 852724 ptree@ab-direct.com www.ab-direct.com Before you PRINT this e-mail, think about the ENVIRONMENT. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of birnbaumm@asaf.health.gov.il Sent: Wednesday, December 30, 2009 10:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Probe for mouse Y chromosome for FISH Dear all We look for Fluorescence probe of mouse Y chromosome. Which company does sale it? Mira Birnbaum Pathology Asaf Hrofeh Medical Center Israel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet MorphoSys UK Ltd., Registered in England No: 1604642 Registered office: Greyfriars Court, Paradise Square, Oxford OX1 1BB, UK This message may contain confidential and/or privileged information. If you are not the addressee or authorized to receive this for the addressee, you must not use, copy, disclose or take any action based on this message or any information herein. If you have received this message in error, please advise the sender immediately by reply e-mail and delete this message. Thank you for your cooperation. Diese E-Mail kann vertrauliche und/oder rechtlich geschuetzte Informationen enthalten. Wenn Sie nicht der richtige Adressat sind oder diese E-Mail irrtuemlich erhalten haben, informieren Sie bitte sofort den Absender und vernichten Sie diese Mail. Das unerlaubte Kopieren sowie die unbefugte Weitergabe dieser Mail ist nicht gestattet. From rjbuesa <@t> yahoo.com Thu Dec 31 07:58:43 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 31 07:58:47 2009 Subject: [Histonet] Formalin pigment? In-Reply-To: <786f96610912301656o71e21b25vae62e9ff644de4ed@mail.gmail.com> Message-ID: <103545.48284.qm@web65713.mail.ac4.yahoo.com> First you should acquire a field micrometer. One of those that have all the field of view covered by s lattice of crossed lines forming squares. Using a low magnification objective (10:1) count in how many squares you see the pigment and calculate in what % of the total number of squares they are. Repeat in at least 10 areas of the section selected at random, and you will have a percentage idea of how much pigment is in your section?for the time the specimen was kept in formalin at any given concentraiton. Ren? J. --- On Wed, 12/30/09, Adeluwoye Oluwatosin wrote: From: Adeluwoye Oluwatosin Subject: [Histonet] Formalin pigment? To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 30, 2009, 7:56 PM Hello, please i am working effects of various concentration of formaldehyde on liver tissue. The sections shows some degree of formalin pigmentation form 20 to 35% and concentrated formaldehyde. Can anyone ofer advise as to how to quantittate o judge the varying degree of pigmentation? Tosin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mmccoy <@t> lakelandregional.org Thu Dec 31 09:23:07 2009 From: mmccoy <@t> lakelandregional.org (Mary McCoy) Date: Thu Dec 31 09:22:10 2009 Subject: [Histonet] FNA code In-Reply-To: References: Message-ID: <4B3C7B3E.873D.00AB.0@lakelandregional.org> There is no Technical component to 88173, so how do you charge for the technical preparation of the ThinPrep? Mary McCoy HTL(ASCP) Supervisor of Pathology Services Lakeland Regional Health System St. Joseph MI 49098 (269) 982-4891 mmccoy@lakelandregional.org >>> 12/30/2009 9:42 AM >>> 88173 Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Demarinis, Carolyn" Sent by: histonet-bounces@lists.utsouthwestern.edu 12/30/2009 08:18 AM To cc Subject [Histonet] FNA code Which CPT code are labs using for fine needle aspirations that are processed using thinprep technique - FNA interpretation and report-88173 or thinprep non-gyn 88112? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:McCoy, Mary TEL;WORK:982-4891 ORG:;Lab TEL;PREF;FAX:98-8258 EMAIL;WORK;PREF;NGW:MMcCOY@lakelandregional.org N:McCoy;Mary TITLE:Coordinator Pathology END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:McCoy, Mary TEL;WORK;PREF:982-4891 ORG:;Lab TEL;PREF;FAX:98-8258 EMAIL;WORK;PREF;NGW:MMcCOY@lakelandregional.org N:McCoy;Mary TITLE:Coordinator Pathology END:VCARD From Dorothy.L.Webb <@t> HealthPartners.Com Thu Dec 31 12:07:27 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Dec 31 12:07:39 2009 Subject: [Histonet] cryostats In-Reply-To: References: Message-ID: <65365F35C0F2EF4D846EC3CA73E49C43C56FA3984E@HPEMX3.HealthPartners.int> We defrost ours quarterly or more often if needed! We wipe it out daily with absolute alcohol and do a decontamination as necessary. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, December 31, 2009 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 73, Issue 40 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. cap question amp.12087 (anita dudley) 2. AW: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome (Gudrun Lang) 3. Auto Reply (Baustin) 4. Sr HTL opportunity in NC (jeri@opssearchgroup.com) 5. RE: Histonet Digest, Vol 73, Issue 39, Question 10. Dermlab (Valantou Grover) 6. Re: Histonet Digest, Vol 73, Issue 39 (rgrow@bmnet.com) 7. Job In Northern NY (Alyssa Peterson) 8. Path Specimen Source Files (Feher, Stephen) 9. Formalin pigment? (Adeluwoye Oluwatosin) 10. Re: 2x3 microtomy: Adapter vs Sliding Microtome (Anne van Binsbergen) 11. RE: Probe for mouse Y chromosome for FISH (Peter Tree) 12. Re: Formalin pigment? (Rene J Buesa) 13. Re: FNA code (Mary McCoy) ---------------------------------------------------------------------- Message: 1 Date: Wed, 30 Dec 2009 12:03:36 -0600 From: anita dudley Subject: [Histonet] cap question amp.12087 To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" just wondering if everyone is defrosting their cryostats every week? seems like overkill, we clean ours out daily and every week clean with 100 alc. what are others doing? this is a revised question. thanks a lot anita dudley providence hospital mobile _________________________________________________________________ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. http://clk.atdmt.com/GBL/go/171222985/direct/01/ ------------------------------ Message: 2 Date: Wed, 30 Dec 2009 19:18:54 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome To: "'R C'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <393235095CC64FF18D6F370D21E71EEA@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Have you tried to float the section first in a roomtemp. waterbath (bowl with tapwater)? There you can flatten the wrinkles with a brush, then mount it on the glass slide and bring it into the warm water. While gliding from the glass you can also use the brush to pull softly on the section to stretch it. If the infiltrated tissue is still too soft, I suggest longer infiltration time in xylen and paraffin to get rid of the fatty part of brain tissue. Sometimes even cutting on next day of embedding (one day waiting) helps. Do you cool the blocks before cutting? Long enough? For big blocks we usually use one blade for trimming and a new one for getting a thin section. Hope this helps Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von R C Gesendet: Dienstag, 29. Dezember 2009 23:16 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome Hi. I'm working on a protocol for cutting monkey brain sections to be mounted on 2x3 slides. I've read about utilizing a sliding microtome but in short, have decided to use the 2x3 adapter for a standard Microm microtome. During microtomy I've noticed many wrinkles in the sections, particularly within the folds of the cerebellum. The wrinkles worsen as the sections float in the water bath (temp=38). In troubleshooting, a co-worker suggests inadequate fixation. I on the other hand believe that the wrinkles relate to the Type R paraffin, which contains polymers as well as the use of the adapter versus the sliding microtome. Can anyone offer any first hand experience/guidance? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Wed, 30 Dec 2009 13:29:09 -0500 From: Baustin Subject: [Histonet] Auto Reply To: Message-ID: <1000237988@mail-server.cbgbiotech.com> Content-Type: text/plain; charset="utf-8" Please do not respond to this automatic e-mail reply. I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies@cbgbiotech.com or fax it to 614-863-1676 Attn Ordering. If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225. Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG. ------------------------------ Message: 4 Date: Wed, 30 Dec 2009 13:39:13 -0500 From: Subject: [Histonet] Sr HTL opportunity in NC To: Message-ID: <87140935C4DA4270AE1356959FA8558E@D3RWK391> Content-Type: text/plain; charset="iso-8859-1" Now that 2009 is coming to a close, some of you might have already made a new year's resolution to find a more challenging HTL opportunity. If that is true, please note this great opportunity in a wonderful city in NC. The right candidate will take a lead role in the development and implementation of new procedures, instrumentation, computer functionality, etc. Assumes responsibility for one or more major on-going laboratory projects or functions or the equivalent - i.e. CQI, safety, instrumentation, quality control, regulatory, administrative, staff development, computer (LIS,HIS, hospital intranet, PC applications) etc. Serves as a resource in area of responsibility. Performs testing that requires specialized knowledge within a discipline and/or crosses disciplines. Trains new employees in the theoretical and operational aspects, procedures and evaluates their work. Acts as a resource for less experienced co-workers. Assists supervisor/manager in review of laboratory reports, procedures, quality control. 5 year experience in Histology Department to include immuno histochemistry. Bachelors Degree HT or HTL (ASCP) certification. I would be happy to share more details with anyone who might be interested. Jeri Vitello OPS Search Group 574.633.1231 www.opssearchgroup.com jeri@opssearchgroup.com where OPPORTUNITY and PEOPLE meet SUCCESSFULLY ------------------------------ Message: 5 Date: Wed, 30 Dec 2009 14:28:20 -0500 From: "Valantou Grover" Subject: [Histonet] RE: Histonet Digest, Vol 73, Issue 39, Question 10. Dermlab To: Message-ID: <2C12F4C64F364FCEBCAFBDC0068CA1E7@USWARD13ZFB71> Content-Type: text/plain; charset="us-ascii" Rebecca, There are many companies that will set up the lab from beginning to end, TBS, Inc. has this service. You can contact them at 919-384-9393. Valantou Grover, HT/HTL(ASCP), PA, MBA Biosciences Product Line Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 Fax: 1-800-343-3291 Phone number: 1-800-523-2575 X7418 Direct:1-215-488-7418 Cell phone: 1-215-409-8327 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 73, Issue 39 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Auto Reply (Baustin) 2. Part Time MOHS Tech Needed in Portland (Eric Weber) 3. Re: Recycling Formalin, Xylene, and Alcohol (rgrow@bmnet.com) 4. 2x3 microtomy: Adapter vs Sliding Microtome (R C) 5. Re: Stain to differentiate Hemoglobin from Hemosiderin? (Robert Richmond) 6. LEICA 2055 AUTOCUT (Lucy Zong) 7. Re: Stain to differentiate Hemoglobin from Hemosiderin? (John Kiernan) 8. Probe for mouse Y chromosome for FISH (birnbaumm@asaf.health.gov.il) 9. FNA code (Demarinis, Carolyn) 10. Derm Lab (Eric Sulkosky) 11. Re: FNA code (DKBoyd@chs.net) 12. Re: Probe for mouse Y chromosome for FISH (Xipamanine Mkuze) 13. RE: LEICA 2055 AUTOCUT (Feher, Stephen) 14. Mississippi Histotechnology Homecoming (Jerry Santiago) ---------------------------------------------------------------------- Message: 1 Date: Tue, 29 Dec 2009 13:06:33 -0500 From: Baustin Subject: [Histonet] Auto Reply To: Message-ID: <1000171728@mail-server.cbgbiotech.com> Content-Type: text/plain; charset="utf-8" Please do not respond to this automatic e-mail reply. I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies@cbgbiotech.com or fax it to 614-863-1676 Attn Ordering. If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225. Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG. ------------------------------ Message: 2 Date: Tue, 29 Dec 2009 14:53:37 -0500 From: Eric Weber Subject: [Histonet] Part Time MOHS Tech Needed in Portland To: Message-ID: <9D12D4EF30176D4F839EB47F3D0E843611FAAF83@exbk2.maxhealth.com> Content-Type: text/plain; charset="us-ascii" MOHs Tech needed at the Portland VAMC for a Part Time Basis. Please contact me if you would be interested. Details: Dermatology MOHS Health Technician The Portland VA Medical Center is in need of one part-time Dermatology MOHS technician for the Dermatology Clinic, Operative Care Program at the Portland VA Medical Center, 3710 SW US Veterans Hospital Road, Portland, OR 97239. Qualifications: Knowledge of basic methods and procedures of the Dermatology clinic. Candidate shall demonstrate skill and precision in use of the tools, materials and equipment for Dermatology clinic. Knowledge of specialized terminology used in the specialized area of the facility where the work is performed. Strong customer service skills; detail oriented; and adaptability to work in a fast paced work environment. Candidate will serve as technical expert in the operation and maintenance of specialized equipment and instruments. Experience: Basic computer knowledge. Candidate shall demonstrate experience and skill in cutting and preparing frozen sections of tissue in a Dermatology MOHS clinic. Familiarity with medical terminology and familiarity with routine laboratory values and abbreviations are required. Specialty Experience (a Plus but not required): Familiarity with the VA electronic medical record system (CPRS). Length of Assignment: One year, or longer. On-Call Hours: Not available Overtime: Not available Tour of Duty: Mondays for 8 hours, (7:30am to 3:30pm), Fridays for 5 hours (7:30 am to 12:30 pm). Eric Weber Maxim Government Services 7227 Lee DeForest Dr Columbia, MD 21046 phone: (410) 910-4942 toll free: (866) 260-9142 fax:(410) 953-8358 erweber@maxhealth.com CONFIDENTIALITY NOTE: This e-mail message contains confidential, privileged or otherwise protected information intended solely for the addressee. Please do not read, copy, or disseminate it unless you are the intended addressee. If you have received it in error, please call us (collect) at (410) 910-1500 and ask to speak with the message sender. Also, we would appreciate your forwarding the message back to us and deleting it from your system. Thank you. This e-mail and all other electronic (including voice) communications from the sender's firm are for informational purposes only. No such communication is intended by the sender to constitute either an electronic record or an electronic signature or to constitute any agreement by the sender to conduct a transaction by electronic means. Any such intention or agreement is hereby expressly disclaimed unless otherwise specifically indicated. ------------------------------ Message: 3 Date: Tue, 29 Dec 2009 16:04:22 -0500 From: rgrow@bmnet.com Subject: [Histonet] Re: Recycling Formalin, Xylene, and Alcohol To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Andria, CBG is very reliable. I have one of the large capacity units called: Solv Solv. It's set up to run xylene on one side and alcohol on the other. Very convenient, no flushes to do. I don't recommend recycling formalin, to much fume exposure, testing, rebuffering, etc., but this unit will work for formalin too. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax You wrote: Message: 4 Date: Mon, 21 Dec 2009 14:19:36 -0500 From: "Evans, Andria B" Subject: [Histonet] Recycling Formalin, Xylene, and Alcohol To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I would like to know what everyone out in histoland is using to recycle there solutions. We are looking at switching out the procyclers (that are about 10 years old) to something else. Any Pros/Cons of current methods would be great. Thanks!! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 ------------------------------ Message: 4 Date: Tue, 29 Dec 2009 14:16:04 -0800 From: R C Subject: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0912291416u723bd5d4ya6b93e199b4a3d0@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi. I'm working on a protocol for cutting monkey brain sections to be mounted on 2x3 slides. I've read about utilizing a sliding microtome but in short, have decided to use the 2x3 adapter for a standard Microm microtome. During microtomy I've noticed many wrinkles in the sections, particularly within the folds of the cerebellum. The wrinkles worsen as the sections float in the water bath (temp=38). In troubleshooting, a co-worker suggests inadequate fixation. I on the other hand believe that the wrinkles relate to the Type R paraffin, which contains polymers as well as the use of the adapter versus the sliding microtome. Can anyone offer any first hand experience/guidance? Thanks ------------------------------ Message: 5 Date: Tue, 29 Dec 2009 17:26:22 -0500 From: Robert Richmond Subject: [Histonet] Re: Stain to differentiate Hemoglobin from Hemosiderin? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Jerry Ricks, Research Scientist, University of Washington,Department of Pathology asks: >>I've seen Prussian blue as a stain for hemosiderin but that would also stain hemoglobin.<< The Perls prussian blue reaction occurs with hemosiderin ("stainable iron") but not with hemoglobin, hematin, formalin pigment, malarial pigment, or melanin. It isn't a stain, but a reaction between ferric iron and ferrocyanide ion that produces a dense blue precipitate. The Stainsfile page doesn't really answer your questions, and I'd suggest looking it up in some of the standard textbooks. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 6 Date: Tue, 29 Dec 2009 18:05:10 -0500 From: Lucy Zong Subject: [Histonet] LEICA 2055 AUTOCUT To: histonet@lists.utsouthwestern.edu Message-ID: <8daef62e0912291505l7721b8acw2c339e97a29ddb6f@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I was given a Leica 2055 microtome for our lab, however it did not come with an operators manual. Does anyone have one they could e-mail to me? Thank you ------------------------------ Message: 7 Date: Tue, 29 Dec 2009 20:37:52 -0500 From: John Kiernan Subject: Re: [Histonet] Stain to differentiate Hemoglobin from Hemosiderin? To: JR R Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=windows-1252 Please explain. The iron in haemoglobin is tightly protein-bound and not stainable by histochemical methods for the iron in haemosiderin and ferritin. Red blood cells are, for example, Prussian-blue negative. John Kiernan UWO. London, Canada == == == ----- Original Message ----- From: JR R Date: Monday, December 28, 2009 19:54 Subject: [Histonet] Stain to differentiate Hemoglobin from Hemosiderin? To: histonet@lists.utsouthwestern.edu > > I've seen Prussian blue as a stain for hemosiderin but that would also > stain hemoglobin. > > Any ideas? > > Thanks, > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > _________________________________________________________________ > Hotmail: Trusted email with Microsofts powerful SPAM protection. > http://clk.atdmt.com/GBL/go/177141664/direct/01/____________________________ ___________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 30 Dec 2009 12:17:15 +0200 From: Subject: [Histonet] Probe for mouse Y chromosome for FISH To: Message-ID: <054452CCC076BE4DA21E46AE95E32EC3277BE9@mail2.asaf.health.gov.il> Content-Type: text/plain; charset="windows-1255" Dear all We look for Fluorescence probe of mouse Y chromosome. Which company does sale it? Mira Birnbaum Pathology Asaf Hrofeh Medical Center Israel ------------------------------ Message: 9 Date: Wed, 30 Dec 2009 08:17:46 -0500 From: "Demarinis, Carolyn" Subject: [Histonet] FNA code To: Message-ID: Content-Type: text/plain; charset="us-ascii" Which CPT code are labs using for fine needle aspirations that are processed using thinprep technique - FNA interpretation and report-88173 or thinprep non-gyn 88112? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. ------------------------------ Message: 10 Date: Wed, 30 Dec 2009 09:40:09 -0500 From: Eric Sulkosky Subject: [Histonet] Derm Lab To: histonet@lists.utsouthwestern.edu Message-ID: <6c3840890912300640p70132627w9befa4fcca15117f@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 RAJ, What tips are you looking for? Are you starting from scratch with an empty room or has the room already been equipped with ventilation, plumbing and electrical? Eric ------------------------------ Message: 11 Date: Wed, 30 Dec 2009 09:42:03 -0500 From: DKBoyd@chs.net Subject: Re: [Histonet] FNA code To: "Demarinis, Carolyn" Cc: histonet-bounces@lists.utsouthwestern.edu, HISTONET@PATHOLOGY.SWMED.EDU Message-ID: Content-Type: text/plain; charset="US-ASCII" 88173 Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Demarinis, Carolyn" Sent by: histonet-bounces@lists.utsouthwestern.edu 12/30/2009 08:18 AM To cc Subject [Histonet] FNA code Which CPT code are labs using for fine needle aspirations that are processed using thinprep technique - FNA interpretation and report-88173 or thinprep non-gyn 88112? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 12 Date: Wed, 30 Dec 2009 16:37:58 +0100 From: Xipamanine Mkuze Subject: Re: [Histonet] Probe for mouse Y chromosome for FISH To: birnbaumm@asaf.health.gov.il Cc: histonet@lists.utsouthwestern.edu Message-ID: <3cfdeb590912300737g155fbd26v1e2a7b0ed98d387d@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Cambio (http://www.cambio.co.uk) 2009/12/30 > Dear all > > We look for Fluorescence probe of mouse Y chromosome. Which company does > sale it? > > Mira Birnbaum > > Pathology > > Asaf Hrofeh Medical Center > > Israel > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Wed, 30 Dec 2009 11:43:31 -0500 From: "Feher, Stephen" Subject: RE: [Histonet] LEICA 2055 AUTOCUT To: "Lucy Zong" , Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B74914@exchange.cmc-nh.org> Content-Type: text/plain; charset="us-ascii" I have one for the RM2255 if you think that will help. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucy Zong Sent: Tuesday, December 29, 2009 6:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LEICA 2055 AUTOCUT I was given a Leica 2055 microtome for our lab, however it did not come with an operators manual. Does anyone have one they could e-mail to me? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 30 Dec 2009 09:08:15 -0800 (PST) From: Jerry Santiago Subject: [Histonet] Mississippi Histotechnology Homecoming To: histonet@lists.utsouthwestern.edu Message-ID: <861067.47883.qm@web180409.mail.gq1.yahoo.com> Content-Type: text/plain; charset=utf-8 Mississippi Histotechnology Homecoming Calling all Histotechnologists from the State of Mississippi. The awaited homecoming for the Mississippi Society of Histotechnology is finally here.B The meeting information will be available in January 2010. To receive the information, please send an e-mail with name and address to my attention to: jsantiago@bellsouth.net. Event: Mississippi Histotechnology Homecoming Event Dates: March 12 b ------------------------------ Message: 6 Date: Wed, 30 Dec 2009 14:51:42 -0500 From: rgrow@bmnet.com Subject: [Histonet] Re: Histonet Digest, Vol 73, Issue 39 To: histonet@lists.utsouthwestern.edu Cc: ruebenjcarter@gmail.com Message-ID: Content-Type: text/plain; charset=US-ASCII R C, In past years, I've section many primate brains in normal size sections but not whole, so this may/may not help. If it was fixation you would not be able to get a complete section in the first place, so your fixation should not be a problem. If it was the adaptor on your microtome you would have other sectioning problems: wobble, thick/thin, washboard, curved sections, uneven thickness, etc. A couple of suggestions that might help. The suggestions should work regardless of section thickness. 1. Try initially laying your ribbon on a dish of COLD water. With fine forceps, (chilled too) gently stretch out your section. When you are satisfied pick your section up from the cold and transfer it to the warm water to complete the process. Lengthens your cutting time, but works well if you have the time. 2. Be sure to use good qualilty high profile blades, if your stage/faceplate will accomodate them. Surgipath has good ones. For large sections they are more stable. 3. I did use the Surgipath Infiltration Medium for the processor. And, yes, it does make a difference. Contact your Surgipath Sales Rep and request "Infiltration Medium" to set up in your processor. You might be able to get enough for a trial run. Continue to use your "Type R" for embedding. You may need a combination of these things to get the quality you are looking for. Let me know if I can help further. Happy New Year to ALL Histonetters! Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax You wrote: Message: 4 Date: Tue, 29 Dec 2009 14:16:04 -0800 From: R C Subject: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0912291416u723bd5d4ya6b93e199b4a3d0@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi. I'm working on a protocol for cutting monkey brain sections to be mounted on 2x3 slides. I've read about utilizing a sliding microtome but in short, have decided to use the 2x3 adapter for a standard Microm microtome. During microtomy I've noticed many wrinkles in the sections, particularly within the folds of the cerebellum. The wrinkles worsen as the sections float in the water bath (temp=38). In troubleshooting, a co-worker suggests inadequate fixation. I on the other hand believe that the wrinkles relate to the Type R paraffin, which contains polymers as well as the use of the adapter versus the sliding microtome. Can anyone offer any first hand experience/guidance? ------------------------------ Message: 7 Date: Wed, 30 Dec 2009 15:20:35 -0500 From: Alyssa Peterson Subject: [Histonet] Job In Northern NY To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Allied Search Partners is currently accepting resumes for qualified histotechnician/histotechnologist applicants for a laboratory just 30 miles north of Schenectady, NY. Job Description: Histotechnologists/Histotechnicians Shifts: 11am-7pm, Monday-Friday, Full Time. Benefits: Great benefit package included with offer Please submit your resume for prescreening purposes to alyssa@alliedsearchpartners.com Sign On Bonus: Available through Allied Search Partners *All inquiries are always kept confidential* upon resume submission one of our recruiters will call you for an initial phone interview. No resume will be submitted before an initial phone interview. Be sure to visit our website www.alliedsearchpartners.com to submit your job search request, refer a friend for $$Cash Bonus$$, and have your resume reviewed by our career advisors. -- Here's to a 2010 that EXCEEDS expectations! Alyssa Peterson Director of Recruitment O: 888.388.7571 x 102 F: 888.388.7572 *Be sure to visit us on the web at www.alliedsearchpartners.com ------------------------------ Message: 8 Date: Wed, 30 Dec 2009 17:16:36 -0500 From: "Feher, Stephen" Subject: [Histonet] Path Specimen Source Files To: Message-ID: <73A7ED895EE0C24D9267ED814911DF1912B74922@exchange.cmc-nh.org> Content-Type: text/plain; charset="us-ascii" I am interested to know if any of you out there might have a fairly comprehensive set of Pathology Specimen source files in either Word or Excel. We are in the process of training our Registration personnel to be able to register and accession Pathology specimens. By increasing our source files to make it more comprehensive, we should be able to make it an easier, and more precise, task to accession a variety of pathology specimens into our SoftPath system. The source files that came with the system are general in nature so that we can add to them based on a general root system (i.e. Respiratory, Urinary Tract, etc.). Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org ------------------------------ Message: 9 Date: Thu, 31 Dec 2009 00:56:49 +0000 From: Adeluwoye Oluwatosin Subject: [Histonet] Formalin pigment? To: histonet@lists.utsouthwestern.edu Message-ID: <786f96610912301656o71e21b25vae62e9ff644de4ed@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hello, please i am working effects of various concentration of formaldehyde on liver tissue. The sections shows some degree of formalin pigmentation form 20 to 35% and concentrated formaldehyde. Can anyone ofer advise as to how to quantittate o judge the varying degree of pigmentation? Tosin ------------------------------ Message: 10 Date: Thu, 31 Dec 2009 12:52:21 +0400 From: Anne van Binsbergen Subject: Re: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome To: gu.lang@gmx.at Cc: R C , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 i spent many many years handling pig, mouse and human brain, cutting sections on 2x3 slides. the trick is to use the tap water float out before the warm water, also to cut sections thicker than normal - cut at 10-15 and maybe even 20 microns - trouble is at that thickness AND it being animal tissue, the sections wash off in a flash - unless you use adhesive and cook the sections on to the slides gently at 32C for at least overnight i have some 20micron silver stained cerebellum on 2x3 slides here with me right now, 20 years later they are still good as new!! good luck Annie 2009/12/30 Gudrun Lang > Have you tried to float the section first in a roomtemp. waterbath (bowl > with tapwater)? There you can flatten the wrinkles with a brush, then mount > it on the glass slide and bring it into the warm water. While gliding from > the glass you can also use the brush to pull softly on the section to > stretch it. > > If the infiltrated tissue is still too soft, I suggest longer infiltration > time in xylen and paraffin to get rid of the fatty part of brain tissue. > Sometimes even cutting on next day of embedding (one day waiting) helps. > Do you cool the blocks before cutting? Long enough? > > For big blocks we usually use one blade for trimming and a new one for > getting a thin section. > > Hope this helps > Gudrun > > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von R C > Gesendet: Dienstag, 29. Dezember 2009 23:16 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome > > Hi. I'm working on a protocol for cutting monkey brain sections to be > mounted on 2x3 slides. I've read about utilizing a sliding microtome but in > short, have decided to use the 2x3 adapter for a standard Microm microtome. > During microtomy I've noticed many wrinkles in the sections, particularly > within the folds of the cerebellum. The wrinkles worsen as the sections > float in the water bath (temp=38). > > In troubleshooting, a co-worker suggests inadequate fixation. I on the > other > hand believe that the wrinkles relate to the Type R paraffin, which > contains > polymers as well as the use of the adapter versus the sliding microtome. > > Can anyone offer any first hand experience/guidance? > > Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ------------------------------ Message: 11 Date: Thu, 31 Dec 2009 10:03:12 +0000 From: Peter Tree Subject: [Histonet] RE: Probe for mouse Y chromosome for FISH To: "birnbaumm@asaf.health.gov.il" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Mira, Doubtless quite a few companies out there. Try Cambio: http://www.cambio.co.uk/catalogue-Star_FISH_copy_Mouse_Chromosome_Specific_Probes_Biotin_Labelled_br_Concentrated_Format-1-426 Conc Mouse WCP Biotin Chromosome Y Catalogue number 1187-YMB-02 Have a great 2010 Peter Peter Tree Account Manager Custom Antibody Generation AbD Serotec - Your first choice for antibodies! (A Division of MorphoSys) Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxon. OX5 1GE. Tel: +44 (0)1865 852700 Fax: +44 (0)1865 373899 Direct: +44 (0)1865 852724 ptree@ab-direct.com www.ab-direct.com Before you PRINT this e-mail, think about the ENVIRONMENT. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of birnbaumm@asaf.health.gov.il Sent: Wednesday, December 30, 2009 10:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Probe for mouse Y chromosome for FISH Dear all We look for Fluorescence probe of mouse Y chromosome. Which company does sale it? Mira Birnbaum Pathology Asaf Hrofeh Medical Center Israel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet MorphoSys UK Ltd., Registered in England No: 1604642 Registered office: Greyfriars Court, Paradise Square, Oxford OX1 1BB, UK This message may contain confidential and/or privileged information. If you are not the addressee or authorized to receive this for the addressee, you must not use, copy, disclose or take any action based on this message or any information herein. If you have received this message in error, please advise the sender immediately by reply e-mail and delete this message. Thank you for your cooperation. Diese E-Mail kann vertrauliche und/oder rechtlich geschuetzte Informationen enthalten. Wenn Sie nicht der richtige Adressat sind oder diese E-Mail irrtuemlich erhalten haben, informieren Sie bitte sofort den Absender und vernichten Sie diese Mail. Das unerlaubte Kopieren sowie die unbefugte Weitergabe dieser Mail ist nicht gestattet. ------------------------------ Message: 12 Date: Thu, 31 Dec 2009 05:58:43 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Formalin pigment? To: histonet@lists.utsouthwestern.edu, Adeluwoye Oluwatosin Message-ID: <103545.48284.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 First you should acquire a field micrometer. One of those that have all the field of view covered by s lattice of crossed lines forming squares. Using a low magnification objective (10:1) count in how many squares you see the pigment and calculate in what % of the total number of squares they are. Repeat in at least 10 areas of the section selected at random, and you will have a percentage idea of how much pigment is in your section?for the time the specimen was kept in formalin at any given concentraiton. Ren? J. --- On Wed, 12/30/09, Adeluwoye Oluwatosin wrote: From: Adeluwoye Oluwatosin Subject: [Histonet] Formalin pigment? To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 30, 2009, 7:56 PM Hello, please i am working effects of various concentration of formaldehyde on liver tissue. The sections shows some degree of formalin pigmentation form 20 to 35% and concentrated formaldehyde. Can anyone ofer advise as to how to quantittate o judge the varying degree of pigmentation? Tosin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Thu, 31 Dec 2009 10:23:07 -0500 From: "Mary McCoy" Subject: Re: [Histonet] FNA code To: ,, "Carolyn Demarinis" Cc: HISTONET@PATHOLOGY.SWMED.EDU Message-ID: <4B3C7B3E.873D.00AB.0@lakelandregional.org> Content-Type: text/plain; charset="us-ascii" There is no Technical component to 88173, so how do you charge for the technical preparation of the ThinPrep? Mary McCoy HTL(ASCP) Supervisor of Pathology Services Lakeland Regional Health System St. Joseph MI 49098 (269) 982-4891 mmccoy@lakelandregional.org >>> 12/30/2009 9:42 AM >>> 88173 Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Demarinis, Carolyn" Sent by: histonet-bounces@lists.utsouthwestern.edu 12/30/2009 08:18 AM To cc Subject [Histonet] FNA code Which CPT code are labs using for fine needle aspirations that are processed using thinprep technique - FNA interpretation and report-88173 or thinprep non-gyn 88112? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:McCoy, Mary TEL;WORK:982-4891 ORG:;Lab TEL;PREF;FAX:98-8258 EMAIL;WORK;PREF;NGW:MMcCOY@lakelandregional.org N:McCoy;Mary TITLE:Coordinator Pathology END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:McCoy, Mary TEL;WORK;PREF:982-4891 ORG:;Lab TEL;PREF;FAX:98-8258 EMAIL;WORK;PREF;NGW:MMcCOY@lakelandregional.org N:McCoy;Mary TITLE:Coordinator Pathology END:VCARD ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 73, Issue 40 **************************************** This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From baustin <@t> cbgbiotech.com Thu Dec 31 12:07:53 2009 From: baustin <@t> cbgbiotech.com (Baustin) Date: Thu Dec 31 12:07:57 2009 Subject: [Histonet] Auto Reply Message-ID: <1000302842@mail-server.cbgbiotech.com> Please do not respond to this automatic e-mail reply. I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies@cbgbiotech.com or fax it to 614-863-1676 Attn Ordering. If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225. Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG. From tkngflght <@t> yahoo.com Thu Dec 31 12:55:14 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Dec 31 12:55:19 2009 Subject: [Histonet] IHC cost query Message-ID: <405539.17810.qm@web50907.mail.re2.yahoo.com> Hi Guys- ? Now we're on to cost per slide for automated? Immunohistochemistry questions!? I have to come up with a rough cost to develop a few new antibodies.? I do not have enough information laying around to pull this together myself. ? Could you/would you share the cost per slide as your lab does the tests?? We're automated but whatever you have will contribute to my quest. ? Thanks for sharing--I appreciate all your help!! ? Cheryl Kerry, HT(ASPC) Houston TX From deliadfam <@t> yahoo.com Thu Dec 31 13:26:20 2009 From: deliadfam <@t> yahoo.com (DELIA GARCIA) Date: Thu Dec 31 13:26:24 2009 Subject: [Histonet] Nail softner Message-ID: <302953.65147.qm@web63102.mail.re1.yahoo.com> Hi Everyone, ? I have a question... What is a good nail softner to use at the grossing table prior to fixation? We were currently using KOHw/DMSO from Healthlink Healthcare. However I cannot find who sells it. Healthlink only distributes. Plus I dont have the time to keep hunting for it. I'm hoping there are other alternatives you all might be using that you can share with me. What we had has lasted us my whole time here and then some so I havent had to order it before. Any suggestions would be so greatly appreciated. Thank you in advance. You all have a Happy New Year!!!! ? Delia Lead Histologist From deliadfam <@t> yahoo.com Thu Dec 31 13:29:28 2009 From: deliadfam <@t> yahoo.com (DELIA GARCIA) Date: Thu Dec 31 13:29:33 2009 Subject: [Histonet] Cryostat Message-ID: <919808.82482.qm@web63101.mail.re1.yahoo.com> ? We are a Derm Lab and we use ours quite a bit. We do a manual defrost monthly. We clean it daily. It is also set to auto defrost nightly. ? ? Delia From rjbuesa <@t> yahoo.com Thu Dec 31 14:34:36 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 31 14:34:40 2009 Subject: [Histonet] Fw: Nail softner Message-ID: <148642.39837.qm@web65709.mail.ac4.yahoo.com> Under separate cover, the best procedure ever! Ren? J. --- On Thu, 12/31/09, DELIA GARCIA wrote: From: DELIA GARCIA Subject: [Histonet] Nail softner To: histonet@lists.utsouthwestern.edu Date: Thursday, December 31, 2009, 2:26 PM Hi Everyone, ? I have a question... What is a good nail softner to use at the grossing table prior to fixation? We were currently using KOHw/DMSO from Healthlink Healthcare. However I cannot find who sells it. Healthlink only distributes. Plus I dont have the time to keep hunting for it. I'm hoping there are other alternatives you all might be using that you can share with me. What we had has lasted us my whole time here and then some so I havent had to order it before. Any suggestions would be so greatly appreciated. Thank you in advance. You all have a Happy New Year!!!! ? Delia Lead Histologist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Thu Dec 31 15:10:01 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Dec 31 15:10:12 2009 Subject: [Histonet] FNA code In-Reply-To: <4B3C7B3E.873D.00AB.0@lakelandregional.org> References: <4B3C7B3E.873D.00AB.0@lakelandregional.org> Message-ID: Mary, I'd have to disagree. 88173 has both a technical and professional component. This is well documented in a resource your dept might find very beneficial entitled "Pathology Service Coding Handbook" which is authored by Dennis Padgett, a noted coding authority. It is customary to have a cytotechnologist screen FNA smears or Thin Prep slides prior to the slides going to sign out with the pathologist. And of course the hospital incurs supply expenses as well as labor expense associated with the preparation of the specimen(s) that will be interpreted The rule of thumb is as follows: 88173 should be dropped for every Fine Needle Aspirate. This represents all of the work in the laboratory (including prep and staining) that leads to the interpretation both technical and professional 88172 is dropped only if the procedure is attended by a pathologist or cytotechnologist to render an adequacy during the procedure. This presumes that the aspirate is not being performed by the pathologist but another clinician, radiologist, etc. It is appropriate to drop additional codes to 88173, such as codes for cell block (88305), special stains (88312;88313), IHC (88342) or thin layer preparation of the aspirate (88112 ) however it may interest you to know that, according to Padgett, Medicare will never pay 88112 when it is added onto 88173. so for example, if you do direct smears and thin prep on an FNA specimen, Medicare will still only pay for 88173. Other payors may vary and for simplicity it may be simpler for labs to drop 88112 whenever thin prep is done on a FNA specimen without regard to payor however you ought to consult with your institution's legal or compliance officer. I highly recommend the reference I mentioned above. You wouldn't believe the complexity of the interpretations for these codes and Padgett points out instances where CAP's interpretation disagrees with AMA's. Padgett devotes 21 pages of discussion on FNA coding Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary McCoy Sent: Thursday, December 31, 2009 10:23 AM To: DKBoyd@chs.net; histonet-bounces@lists.utsouthwestern.edu; Carolyn Demarinis Cc: HISTONET@PATHOLOGY.SWMED.EDU Subject: Re: [Histonet] FNA code There is no Technical component to 88173, so how do you charge for the technical preparation of the ThinPrep? Mary McCoy HTL(ASCP) Supervisor of Pathology Services Lakeland Regional Health System St. Joseph MI 49098 (269) 982-4891 mmccoy@lakelandregional.org >>> 12/30/2009 9:42 AM >>> 88173 Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkboyd@chs.net "Demarinis, Carolyn" Sent by: histonet-bounces@lists.utsouthwestern.edu 12/30/2009 08:18 AM To cc Subject [Histonet] FNA code Which CPT code are labs using for fine needle aspirations that are processed using thinprep technique - FNA interpretation and report-88173 or thinprep non-gyn 88112? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Dec 31 16:09:32 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Dec 31 16:09:48 2009 Subject: [Histonet] Nail softner In-Reply-To: <302953.65147.qm@web63102.mail.re1.yahoo.com> References: <302953.65147.qm@web63102.mail.re1.yahoo.com> Message-ID: 20% sodium hydroxide works great. Hey, they don't call me "Joe the Toe" for nothing. Happy New Year JTT ----- Original Message ----- From: "DELIA GARCIA" To: Sent: Thursday, December 31, 2009 1:26 PM Subject: [Histonet] Nail softner Hi Everyone, I have a question... What is a good nail softner to use at the grossing table prior to fixation? We were currently using KOHw/DMSO from Healthlink Healthcare. However I cannot find who sells it. Healthlink only distributes. Plus I dont have the time to keep hunting for it. I'm hoping there are other alternatives you all might be using that you can share with me. What we had has lasted us my whole time here and then some so I havent had to order it before. Any suggestions would be so greatly appreciated. Thank you in advance. You all have a Happy New Year!!!! Delia Lead Histologist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Dec 31 16:17:16 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Dec 31 16:17:48 2009 Subject: [Histonet] Nail softner In-Reply-To: <302953.65147.qm@web63102.mail.re1.yahoo.com> References: <302953.65147.qm@web63102.mail.re1.yahoo.com> Message-ID: We used to put our nails in Nail Hair remover for over night. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DELIA GARCIA [deliadfam@yahoo.com] Sent: Thursday, December 31, 2009 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nail softner Hi Everyone, I have a question... What is a good nail softner to use at the grossing table prior to fixation? We were currently using KOHw/DMSO from Healthlink Healthcare. However I cannot find who sells it. Healthlink only distributes. Plus I dont have the time to keep hunting for it. I'm hoping there are other alternatives you all might be using that you can share with me. What we had has lasted us my whole time here and then some so I havent had to order it before. Any suggestions would be so greatly appreciated. Thank you in advance. You all have a Happy New Year!!!! Delia Lead Histologist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Dec 31 16:21:49 2009 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Dec 31 16:22:16 2009 Subject: [Histonet] Nail softner In-Reply-To: References: <302953.65147.qm@web63102.mail.re1.yahoo.com>, Message-ID: Sorry I mean Nair Hair Remover. Any scent you like. Happy new Year. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A [Loralee_Mcmahon@URMC.Rochester.edu] Sent: Thursday, December 31, 2009 5:17 PM To: DELIA GARCIA; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Nail softner We used to put our nails in Nail Hair remover for over night. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DELIA GARCIA [deliadfam@yahoo.com] Sent: Thursday, December 31, 2009 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nail softner Hi Everyone, I have a question... What is a good nail softner to use at the grossing table prior to fixation? We were currently using KOHw/DMSO from Healthlink Healthcare. However I cannot find who sells it. Healthlink only distributes. Plus I dont have the time to keep hunting for it. I'm hoping there are other alternatives you all might be using that you can share with me. What we had has lasted us my whole time here and then some so I havent had to order it before. Any suggestions would be so greatly appreciated. Thank you in advance. You all have a Happy New Year!!!! Delia Lead Histologist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet