From Robert.Lott <@t> trinitymedicalonline.com Sat Aug 1 08:20:05 2009 From: Robert.Lott <@t> trinitymedicalonline.com (Lott, Robert) Date: Sat Aug 1 08:20:14 2009 Subject: [Histonet] Histonet DIGEST subscribers ONLY - PLEASE read Message-ID: <4A3619571D9F6C4CB79C980E91DBE4E6012B83A4@TNTRIEXEVS03.triadhospitals.net> I apologize to all NON-Digest Histonetters..... please do not read further. For those of us who enjoy the DIGEST format....like me... Please, please, please DO NOT use your "REPLY" or your "REPLY TO ALL" button(s) inside your e-mail client when replying to a message inside the DIGEST. When you do... you copy the entire list back with your reply. Someone else does it and now you have a list within another list...within another list... etc, etc, within the list you are reading.... UNDERSTAND?? This is what makes the digest so repetitive!! ------------------------------------------------------------------------ ---- To "Reply" or to "Reply to All", you should "copy" (highlight, right click, and Copy) their message out of the DIGEST and then create a new e-mail message.... this way you are not copying the entire list back within your "REPLY" or your "REPLY to ALL". In addition, my e-mail client has an option to detach the received message from the one you are sending... or replying too. Please help make the DIGEST format of histonet a better experience! Many DIGEST subscribers have described this problem in the past, but the repetitiveness lately has prompted me to jump into the fray...again. Flame away!!! Robert Robert L. Lott, HTL(ASCP) / Manager, Anatomic Pathology Trinity Medical Center/LabFirst / Birmingham, AL 35213-1984 205-592-5388 Robert L. Lott, HTL(ASCP) / Manager, Anatomic Pathology Trinity Medical Center/ LabFirst/ 800 Montclair Road / Birmingham, AL 35213 / 205-592-5388 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From boneslides <@t> aol.com Sat Aug 1 09:40:12 2009 From: boneslides <@t> aol.com (boneslides@aol.com) Date: Sat Aug 1 09:40:25 2009 Subject: [Histonet] Poly Slides? Message-ID: <8CBE0CD1C9DC001-724-24C8@webmail-md04.sysops.aol.com> Now that Wasatch Histology is closed (Happy Retirement Cathy!!!), does anyone have another source for poly slides? Thanks! Diane Mahovlic Orthopedic Pathology & Biomaterials Laboratory Cleveland Clinic Cleveland, Ohio From AnthonyH <@t> chw.edu.au Sun Aug 2 17:46:53 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 2 17:47:09 2009 Subject: [Histonet] Tissue Washing In-Reply-To: Message-ID: Several ideas - 1. Make sure the biopsies do not dry before adding to the fixative. 2. Extend your fixation time as much as possible, decrease your processing times (esp if you are using microwave processing) 3. Dry the slides for 25-30 min at 64oC (60 may not be hot enough to melt the paraffin Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Butte Sent: Friday, 31 July 2009 11:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Washing Our lab has been struggling for a while now, with tissue washing off the slides. The problem was originally traced to our processor that was malfunctioning and having major carry-over issues, causing poor tissue processing. So after failed attempts to get the old machine fixed, we ended up getting a new microwave processor. The tissue (mainly prostate bx) comes out of the processor looking good. It cuts well, but after staining, some of the sections are no longer on the slides. The problem was quiet severe with the old processor and has decreased drastically, but has not entirely gone away with the microwave processor. Here's some info on our current procedure. This is a small physician office lab and we everything manual. We use plus slides and a water bath with no tissue adhesive added. The bath is between 42-45 degrees Celsius. After sectioning, the slide are put in an oven at 60 degrees for 20 minutes. After taking the slides out of the oven, we cool them to room temp then hand stain them in an H&E line. Xylene - 5 min Xylene - 2 min Xylene - 2 min 100% Alcohol - 30 sec 100% Alcohol - 30 sec 100% Alcohol - 30 sec 95% Alcohol - 30 sec Rinse in DiH2O Hematoxylin - 2 min Rinse in DiH2O 0.50% Acid Alcohol - 2 dips Rinse in DiH2O Scott's Tap Water - 10 sec Rinse in DiH2O 95% Alcohol - 30 sec Eosin - 30 sec 95% Alcohol - 30 sec 100% Alcohol - 30 secs 100% Alcohol - 30 secs 100% Alcohol - 30 secs Xylene - 30 sec Xylene 2 min Xylene - 2 min The slides are then cover slipped and of a 12 piece prostate case, at least a section or 2 will wash off of about 4 of the slides. Please pass along any ideas as to what the cause of this could be and how I can fix it. I'm fresh out of ideas and would really appreciate the advice of someone with more experience than myself. Thanks, -- Emily Path Lab Solutions Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Aug 2 17:52:37 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 2 17:52:42 2009 Subject: [Histonet] Please Help! In-Reply-To: <001d01ca1216$39d7b9a0$ce1d41ab@DellDesktop2> Message-ID: Nick, NBF may not work for Delia. She needs to do IF on the frozen sections and NBF fixation will require her to do AR on the sections (esp if she is localising immunoglobulins and complement). Possibly warming the frozen tissue, using a thumb, may help. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicholas David Evans Sent: Saturday, 1 August 2009 5:37 AM To: 'DELIA GARCIA'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Please Help! We fix skin by sandwiching it between two sponges soaked in either 4% PFA or 0.4%PFA (for paraffin or cryo respectively) and compressed in a cassette. This flattens the skin. Following several hours fixation the skin is usually flat and rigid enough to handle and embed. It can then either by dehydrated for paraffin or soaked in sucrose for cryo. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DELIA GARCIA Sent: Friday, July 31, 2009 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Please Help! Hello, I am in dire need of some suggestions. I work for a Derm lab and we occasionally get fresh skin punches that we freeze to do FITC (immunoflouresence). I have a really hard tiime keeping the tissue from curling makeing almost impossible to get a decent section on the slide. Anyone out there that might have experienced the same thing I would love to learn your techniques that overcame this problem. I have tried lightly blowing, freeze spraying, and lightly brushing acetone on the cold plate (not all at the same time). Nothing seems to ease it up. PLEASE HELP! I have one in the cryostat now. Thank you so very much. Delia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Aug 2 17:57:54 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 2 17:58:04 2009 Subject: [Histonet] tissue submission question In-Reply-To: <7DBD932BC2F34598871215B1FCC111D4.MAI@accuwebhosting.biz> Message-ID: Laurie, It is a little fiddly but as long as you keep the animal frozen, you can dissect out the muscle, quickly place it on some OCT on a chuck and freeze the chuck. Try to not let the tissue thaw. How big is the animal? You could do this on the freezing plate of the embedding centre or on the cold shelf in the cryostat. It is important throughout this process to not let the tissue thaw too much. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of laurie@conxis.com Sent: Saturday, 1 August 2009 6:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue submission question Happy Friday everyone. I have an investigator that wants to submit leg muscle from a rat for IHC staining. The problem is they did not dissect the muscle out after euthanizing the animal, they just froze the whole animal. We do not have a sliding microtome so that we can just "slice" the animal up ( yes the investigator called it slicing.) Any thoughts out there? No this is not a Fun Friday joke :-( Have a great weekend! Laurie Laurie Popp, BA HT ( ASCP) **************************************** ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From anand <@t> ethoneuro.com Mon Aug 3 03:10:17 2009 From: anand <@t> ethoneuro.com (Anand Vasudevan) Date: Mon Aug 3 03:10:43 2009 Subject: [Histonet] antifreeze Message-ID: <189449020908030110qae0ec38ta06fdac86d45f1f2@mail.gmail.com> Hi, I am currently doing cryosectioning- free floating sections (50um). After slicing I place the sections in a well plate containing PBS. I was thinking of using an antifreeze solution- are there commercial available solutions or do I need to prepare it and if so how to make a working solution? Thanks, Anand Nanyang Technological University Singapore From Kathleen.Caleri <@t> RoswellPark.org Mon Aug 3 07:04:09 2009 From: Kathleen.Caleri <@t> RoswellPark.org (Caleri, Kathleen) Date: Mon Aug 3 07:03:07 2009 Subject: [Histonet] autostainer Message-ID: Hello-we are considering purchasing an autostainer for special stains....I would appreciate some input on the units from Dako and Ventana...thanks! This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From sjvinay <@t> yahoo.com Mon Aug 3 09:59:54 2009 From: sjvinay <@t> yahoo.com (vinay sj) Date: Mon Aug 3 09:59:58 2009 Subject: [Histonet] immunocytochemistry for Systems biology Message-ID: <847950.99010.qm@web54207.mail.re2.yahoo.com> Dear Histonetters, I am working on Systems biology project for the quantification of some phospoproteins using a phospho-specific antibody. As is the case with life in general, there are problems. So, I need your expert advice. My experimental protocol: fix with 4 % Paraformaldehyde, permeabilize with 0.2 %Triton, Block with goat serum, stain with primary antibody and secondary antibody( raised in goat). >From flow cytometry experiments, I see that there is a background staining when i do the immunocytochemistry without applying my stimulus(i.e pfa+ triton+antibodies). From western blots, I know that without stimulus, there is no phosphoproteins and hence the phospho signal ought to be zero. The background staining is 5 fold higher than plain autoflourescence(pfa+triton) and 3 fold higher than autoflourescence due to secondary antibody(pfa+triton+secondary antibody). My phospho signal is around 4 fold higher than my background signal. ?I do not understand how to eliminate the robust primary antibody nonspecific staining. I do not want to use methanol, since I would like to do the experiments in 96 well format as well. I have read that aldehyde fixation could give rise to non specific signals. But I want to stick to Paraformaldehyde, since I cannot cool my 96 well plates, to use methanol. Any help will be appreciated. Regards, Vinay. From Vickroy.Jim <@t> mhsil.com Mon Aug 3 10:14:18 2009 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Aug 3 10:14:23 2009 Subject: [Histonet] NEED GLASS DOOR FOR SHANDON CRYOTOME Message-ID: <24A4826E8EF0964D86BC5317306F58A52BC211C2B8@mmc-mail.ad.mhsil.com> Looking for a glass door on a Shandon Cryotome. Older model. Anybody have any good ideas on where we should look? Jim Vickroy Memorial Medical Center Springfield, Illinois 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From cls71877 <@t> sbcglobal.net Mon Aug 3 12:03:15 2009 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Mon Aug 3 12:03:18 2009 Subject: [Histonet] GI lab Message-ID: <219191.85920.qm@web81201.mail.mud.yahoo.com> Hello all, I am currently setting up a new lab devoted to GI biopsies.? I have a few questions I was hoping someone could help me with.? Are HT?s allowed to accession under CLIA as long as it is only a physical description?? What standard protocols for special stains do other labs currently use concerning specific site specimens?? (ex. Giemsa on gastric biopsies, etc.,)? Any other suggestions and insights are greatly appreciated as well.? Thank you in advance for your time! Cristi Stephenson MSA, HT(ASCP) ? From jstaruk <@t> masshistology.com Mon Aug 3 12:59:16 2009 From: jstaruk <@t> masshistology.com (jstaruk) Date: Mon Aug 3 12:58:57 2009 Subject: [Histonet] Lab certification In-Reply-To: Message-ID: <62F9C9F4434340C792C1D6FE1A5AB7D8@JimPC> Occasionally I have potential clients ask if our lab is "GLP certified"? I explain that we follow all GLP guidelines in accordance with the Food & Drug Administration's regulations #21 CFR, Part 58 (Good Laboratory Practice For Non Clinical Laboratory Studies) and that we have passed a 3-day FDA audit, but they actually don't hand you a piece of paper saying you are "certified". Is there a certifying agency that inspects private histopathology labs and then crowns you "GLP certified"? Thanks Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com From cls71877 <@t> sbcglobal.net Mon Aug 3 13:39:02 2009 From: cls71877 <@t> sbcglobal.net (Cristi stephenson) Date: Mon Aug 3 13:39:05 2009 Subject: [Histonet] WindoPath Message-ID: <428592.32791.qm@web81202.mail.mud.yahoo.com> Hi all, Does anyone out there currently use WindoPath as their LIS?? Any opinions or pointers concerning set-up, usage and the like? Thanks in advance?for your guidance, Cristi Stephenson MSA, HT(ASCP)? From NMargaryan <@t> childrensmemorial.org Mon Aug 3 15:19:03 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Aug 3 15:19:27 2009 Subject: [Histonet] reagents for IHC Message-ID: Hi everyone, How are you experiencing the economic pressures and price changes for REAGENTS? I am sorry, but I just bought reagents from DAKO and, for the price I paid for 125 ml before, I got 15-50 ml :( I am ready to switch to another company, but I need your suggestion about reagents for IHC and companies you are purchasing from: Peroxidase Block, Protein Block, Antibody diluents, DAB, AEC, different secondaries and tertiaries. Thanks in advance, Naira From mucram11 <@t> comcast.net Mon Aug 3 15:28:27 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Mon Aug 3 15:28:35 2009 Subject: [Histonet] reagents for IHC In-Reply-To: References: Message-ID: <001201ca1478$f598bfe0$e0ca3fa0$@net> Talk to Innovex!! I really like the company and the technical help as well as the pricing. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Monday, August 03, 2009 4:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] reagents for IHC Hi everyone, How are you experiencing the economic pressures and price changes for REAGENTS? I am sorry, but I just bought reagents from DAKO and, for the price I paid for 125 ml before, I got 15-50 ml :( I am ready to switch to another company, but I need your suggestion about reagents for IHC and companies you are purchasing from: Peroxidase Block, Protein Block, Antibody diluents, DAB, AEC, different secondaries and tertiaries. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Mon Aug 3 15:29:29 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Aug 3 15:29:54 2009 Subject: [Histonet] reagents for IHC In-Reply-To: Message-ID: Biocare. Biocare. Biocare. "Margaryan, Naira" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/03/2009 03:19 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] reagents for IHC Hi everyone, How are you experiencing the economic pressures and price changes for REAGENTS? I am sorry, but I just bought reagents from DAKO and, for the price I paid for 125 ml before, I got 15-50 ml :( I am ready to switch to another company, but I need your suggestion about reagents for IHC and companies you are purchasing from: Peroxidase Block, Protein Block, Antibody diluents, DAB, AEC, different secondaries and tertiaries. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Mon Aug 3 15:34:59 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Aug 3 15:35:04 2009 Subject: [Histonet] reagents for IHC In-Reply-To: References: Message-ID: <93300.88617.qm@web1106.biz.mail.sk1.yahoo.com> Hi, I buy my 3% H2O2 at Walmart or Walgreens. $1.50/quart. I try to buy?all other detection supplies?from Vector Labs. www.vectorlabs.com Plus they only charge actual shipping prices! Paula ________________________________ From: "Margaryan, Naira" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, August 3, 2009 3:19:03 PM Subject: [Histonet] reagents for IHC Hi everyone, How are you experiencing the economic pressures and price changes for REAGENTS? I am sorry, but I just bought reagents from DAKO and, for the price I paid for 125 ml before, I got 15-50 ml :( I am ready to switch to another company, but I need your suggestion about reagents for IHC and companies you are purchasing from: Peroxidase Block, Protein Block, Antibody diluents, DAB, AEC, different secondaries and tertiaries. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gentras <@t> vetmed.auburn.edu Mon Aug 3 16:18:22 2009 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Mon Aug 3 16:18:27 2009 Subject: [Histonet] Re: tissue processor Message-ID: <4A77541E.1000607@vetmed.auburn.edu> hello, I'm in search of the best tissue processor on the market thats' most comparable to the Autotechnicon Ultra II. I specifically need it to be equipped with vacuum & heat controlled processing and for it to possess the capability of processing 2.75" and/or 1.5" stainless steel round tissue cassettes. Prompt reply will be much appreciated. ~ Atoska From Timothy.Morken <@t> ucsfmedctr.org Mon Aug 3 17:16:38 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Mon Aug 3 17:17:09 2009 Subject: [Histonet] RE: reagents for IHC In-Reply-To: References: Message-ID: <1AAF670737F193429070841C6B2ADD4CE04D61F4@EXMBMCB15.ucsfmedicalcenter.org> For IHC ancillary reagents (buffers, diluents, AR reagents), most of which are interchangeable, try the smaller companies, Biocare, Diagnostic Biosystems (dbiosys.net), Cell Marque, Lab Vision (now under Thermo, www.labvision.com), Scytek, among others. They are usually much cheaper since they are trying to get business away from the big companies. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Monday, August 03, 2009 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] reagents for IHC Hi everyone, How are you experiencing the economic pressures and price changes for REAGENTS? I am sorry, but I just bought reagents from DAKO and, for the price I paid for 125 ml before, I got 15-50 ml :( I am ready to switch to another company, but I need your suggestion about reagents for IHC and companies you are purchasing from: Peroxidase Block, Protein Block, Antibody diluents, DAB, AEC, different secondaries and tertiaries. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Mon Aug 3 17:24:59 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Mon Aug 3 17:25:16 2009 Subject: correction FW: [Histonet] RE: reagents for IHC Message-ID: <1AAF670737F193429070841C6B2ADD4CE04D6202@EXMBMCB15.ucsfmedicalcenter.org> Diagnostic BioSystems is www.dbiosys.com and not .net. ----- Original Message ----- From: "Morken, Tim" To: "Margaryan, Naira" ; Sent: Monday, August 03, 2009 3:16 PM Subject: [Histonet] RE: reagents for IHC For IHC ancillary reagents (buffers, diluents, AR reagents), most of which are interchangeable, try the smaller companies, Biocare, Diagnostic Biosystems (dbiosys.net), Cell Marque, Lab Vision (now under Thermo, www.labvision.com), Scytek, among others. They are usually much cheaper since they are trying to get business away from the big companies. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Monday, August 03, 2009 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] reagents for IHC Hi everyone, How are you experiencing the economic pressures and price changes for REAGENTS? I am sorry, but I just bought reagents from DAKO and, for the price I paid for 125 ml before, I got 15-50 ml :( I am ready to switch to another company, but I need your suggestion about reagents for IHC and companies you are purchasing from: Peroxidase Block, Protein Block, Antibody diluents, DAB, AEC, different secondaries and tertiaries. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Mejia <@t> ucsf.edu Mon Aug 3 17:43:55 2009 From: Maria.Mejia <@t> ucsf.edu (Mejia, Maria) Date: Mon Aug 3 17:45:04 2009 Subject: [Histonet] antifreeze In-Reply-To: <189449020908030110qae0ec38ta06fdac86d45f1f2@mail.gmail.com> References: <189449020908030110qae0ec38ta06fdac86d45f1f2@mail.gmail.com> Message-ID: Hello, Here in our lab we section lots of rat & primate (large blocks) of frozen brain tissues. We cut them at 40um as free-floating sections & place each section in wells - using the 24 plate well. The wells have 2ml each of anti-freeze solution which is stored at 4C or -20C for IHC methods. Here is the way we make it. 160ml X2 - PBS 120ml X2 - ethylene glyco 120ml X2 - glycerin mix well & store at 4C Tissues are kept well & IHC is very very good! Hope this helps. Regards Maria Mejia Histology Manager Department of Neurosurgery UCSF San Francisco, CA 94103 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anand Vasudevan [anand@ethoneuro.com] Sent: Monday, August 03, 2009 1:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antifreeze Hi, I am currently doing cryosectioning- free floating sections (50um). After slicing I place the sections in a well plate containing PBS. I was thinking of using an antifreeze solution- are there commercial available solutions or do I need to prepare it and if so how to make a working solution? Thanks, Anand Nanyang Technological University Singapore _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From macveigh <@t> usc.edu Mon Aug 3 17:53:05 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Mon Aug 3 17:53:08 2009 Subject: [Histonet] Re: Immunocytochemistry for Systems biology Message-ID: <005601ca148d$29eb2120$5c237d80@DFS66DD1> Hi Vinay, I am not an expert in this field... We use this protocol for frozen sections and it works. For cells grown on coverslips you skip step #6. Try this : Fix 1. PBS 2 x 3min 2. NH4Cl 5 min (50mM in PBS) (Removes the autofluorescence from the aldehyde groups of the fixative) 3. PBS 2 x 3 min 4. 0.1% Tx-100 5 min (Permeabilises the cell walls) 5. PBS 2 x 3 min 6. SDS 5 min (1% SDS in PBS) (works like antigen retrieval) 7. PBS 3 x 5 min 8. Block 1% BSA in PBS 10 min or longer 9. Primary Ab 40min in 37oC or 1 to 2 hours at room temp 10. PBS 2 x 5 min 11. Secondary Ab 40min in 37oC 12. PBS 2 x 5 min 13. Coverslip with Anti fade medium Let dry overnight Good luck with it Michelle Research specialist USC Kecl School of Medicine From macveigh <@t> usc.edu Mon Aug 3 18:04:21 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Mon Aug 3 18:04:24 2009 Subject: [Histonet] Poly Slides Message-ID: <006d01ca148e$bc6ad530$5c237d80@DFS66DD1> Hi Diane, I used poly coated slydes (which we made in our lab years ago to save money). Then the plus slides came along but are pricey. I just tested and liked a new source for my plus slides which are extremely well priced and worked just like the FISHER and VWR slides... only for half the price :-) Try www.LabMed.biz or call LabMed 877 504-7960 Michelle Research Specialist USC Keck School of Medicine From macveigh <@t> usc.edu Mon Aug 3 18:25:37 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Mon Aug 3 18:25:40 2009 Subject: [Histonet] Re: from PLEASE HELP Message-ID: <007e01ca1491$b54a0160$5c237d80@DFS66DD1> Hi Delia, Some time ago I used to work in a lab where I had to cut skin punch biopsies. They used to come floating in a special preservative. Unfortunately I don't remember its name, but then I had to wash them in a special wash solution for a minute. The wash solution was made by Zeus Scientific and was just citrate buffer. I am not familiar with the Michael's fixative and don't know do you have to wash after fixing. The trick was to pat dry the biopsies with Kimwipes and immerse them right away in the OCT. Then let the biopsy sit there for few minutes. You can even move the tissue in the OCT from time to time and then transfer it to a new mold with fresh OCT. Freeze in liquid Nitrogen. This way, somehow the OCT starts infiltrating the tissue and the tissue cuts together with the OCT. I used to cut it at 5 microns. Hope that this will work for you too Michelle Research Specialist USC Keck School of Medicine From anand <@t> ethoneuro.com Tue Aug 4 00:38:35 2009 From: anand <@t> ethoneuro.com (Anand Vasudevan) Date: Tue Aug 4 00:39:00 2009 Subject: [Histonet] antifreeze Message-ID: <189449020908032238n13051704l233aef82473d1c01@mail.gmail.com> Hi, I am query regarding composition of antifreeze used for storing free floating sections. I know that one component is polyethylene glycol- I understand that a specific type of it is required, that is, a polymer of certain length to make antifreeze solution? Is this correct information? If anyone could tell me what type/brand of ethylene glycol they use, that would be great. Thanks, Anand Vasudevan Nanyang Technological University Singapore From lblazek <@t> digestivespecialists.com Tue Aug 4 06:19:57 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Aug 4 06:17:17 2009 Subject: [Histonet] RE: reagents for IHC In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E390894C787A5@IBMB7Exchange.digestivespecialists.com> I agree with BioCare BioCare BioCare. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Monday, August 03, 2009 4:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] reagents for IHC Hi everyone, How are you experiencing the economic pressures and price changes for REAGENTS? I am sorry, but I just bought reagents from DAKO and, for the price I paid for 125 ml before, I got 15-50 ml :( I am ready to switch to another company, but I need your suggestion about reagents for IHC and companies you are purchasing from: Peroxidase Block, Protein Block, Antibody diluents, DAB, AEC, different secondaries and tertiaries. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Tue Aug 4 08:15:29 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Aug 4 08:16:54 2009 Subject: [Histonet] Re: from PLEASE HELP References: <007e01ca1491$b54a0160$5c237d80@DFS66DD1> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2ADA@fhosxchmb006.ADVENTISTCORP.NET> I believe you are describing the procedure for Michel's Fixative Solution. Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Michelle MacVeigh-Aloni Sent: Mon 8/3/2009 7:25 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: from PLEASE HELP Hi Delia, Some time ago I used to work in a lab where I had to cut skin punch biopsies. They used to come floating in a special preservative. Unfortunately I don't remember its name, but then I had to wash them in a special wash solution for a minute. The wash solution was made by Zeus Scientific and was just citrate buffer. I am not familiar with the Michael's fixative and don't know do you have to wash after fixing. The trick was to pat dry the biopsies with Kimwipes and immerse them right away in the OCT. Then let the biopsy sit there for few minutes. You can even move the tissue in the OCT from time to time and then transfer it to a new mold with fresh OCT. Freeze in liquid Nitrogen. This way, somehow the OCT starts infiltrating the tissue and the tissue cuts together with the OCT. I used to cut it at 5 microns. Hope that this will work for you too Michelle Research Specialist USC Keck School of Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From dea.leslie <@t> gmail.com Tue Aug 4 09:39:57 2009 From: dea.leslie <@t> gmail.com (Deanna Leslie) Date: Tue Aug 4 09:40:02 2009 Subject: [Histonet] ACELL compressed pig bladder Message-ID: Hi to all, Our facility is experimenting with a matrix made from acellular, compressed pig bladder, as an alternative to collagen in the making of engineered skin for burn patients. The name of the material is Matristem Wound Sheet and Matristem Surgical Matrix. It is not very thick, but extremely condensed and somewhat dense, which is the total opposite of the bovine collagen we have been using. During rehydration it was not very hydrophilic, which makes me wonder about my processing times. Has anyone ever worked with this product? If so should I shorten/lengthen processing times? How does it cut? Any information would be greatly appreciated. Thanks, in advance. Deanna Leslie ASCP HT Shriners Hospital for Children Cincinnati, OH 45229 513-872-6388 From NMargaryan <@t> childrensmemorial.org Tue Aug 4 10:32:02 2009 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Tue Aug 4 10:32:28 2009 Subject: [Histonet] RE: reagents for IHC Message-ID: Hi Everyone, I do appreciate for all suggestions I get form most of you! Have a nice week, Naira From nefff <@t> staff.uni-marburg.de Tue Aug 4 10:51:17 2009 From: nefff <@t> staff.uni-marburg.de (Dr. Frauke Neff) Date: Tue Aug 4 10:51:31 2009 Subject: [Histonet] golgi stain- please help Message-ID: <20090804175117.z828f2xw3oow80sg@home.staff.uni-marburg.de> Dear Histonetters, I was performing a golgi stain on mouse brains and my treated animal group gave nice results but the untreated control group did not show any staining at all (it looks like the staining didn't work). Does anyone know / have an idea, if I could repeat the staining on these vibratome sections? I read something about "deimpregnation" of golgi stained slides, but found no protocol and I'm not sure if I can perform another "fresh" golgi stain with these slides. Any suggestion is welcome!!! Thanks to all of you, Frauke From jkiernan <@t> uwo.ca Tue Aug 4 11:27:51 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Aug 4 11:27:56 2009 Subject: [Histonet] golgi stain- please help Message-ID: Golgi staining and related methods are done on whole, fresh pieces of brain tissue, not on sections. There are some techniques for fixed specimens. Instructions can be found in books of neurohistological technique; older ones often have plenty of technical tips. A good start would be: Santini, M., ed. (1975). Golgi Centennial Symposium: Perspectives in Neurobiology. New York: Raven Press. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Dr. Frauke Neff" Date: Tuesday, August 4, 2009 11:52 Subject: [Histonet] golgi stain- please help To: histonet@lists.utsouthwestern.edu > Dear Histonetters, > I was performing a golgi stain on mouse brains and my treated > animal > group gave nice results but the untreated control group did not > show > any staining at all (it looks like the staining didn't work). > Does anyone know / have an idea, if I could repeat the staining > on > these vibratome sections? > I read something about "deimpregnation" of golgi stained slides, > but > found no protocol and I'm not sure if I can perform another > "fresh" > golgi stain with these slides. > > Any suggestion is welcome!!! > > > Thanks to all of you, > > > Frauke > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Tue Aug 4 13:01:28 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Aug 4 13:01:32 2009 Subject: [Histonet] Brucella IHC Message-ID: I'm posting this for a researcher. Does anyone out there do IHC testing for Brucella canis on fixed tissue? If so, please contact me directly. Thanks, Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From making <@t> ufl.edu Tue Aug 4 13:07:02 2009 From: making <@t> ufl.edu (MKing) Date: Tue Aug 4 13:07:08 2009 Subject: [Histonet] Golgi stain Message-ID: <4A7878C6.6090606@ufl.edu> Frauke, There are some published methods for doing Golgi on brain sections: Freund TF, Somogyi P. The section-Golgi impregnation procedure. 1. Description of the method and its combination with histochemistry after intracellular iontophoresis or retrograde transport of horseradish peroxidase. Neuroscience. 1983 Jul;9(3):463-74. Somogyi P, Freund TF, Wu JY, Smith AD. The section-Golgi impregnation procedure. 2. Immunocytochemical demonstration of glutamate decarboxylase in Golgi-impregnated neurons and in their afferent synaptic boutons in the visual cortex of the cat. Neuroscience. 1983 Jul;9(3):475-90. Bolam JP, Ingham CA, Smith AD. The section-Golgi-impregnation procedure--3. Combination of Golgi-impregnation with enzyme histochemistry and electron microscopy to characterize acetylcholinesterase-containing neurons in the rat neostriatum. Neuroscience. 1984 Jul;12(3):687-709. Gabbott PL, Somogyi J. The 'single' section Golgi-impregnation procedure: methodological description. J Neurosci Methods. 1984 Sep;11(4):221-30. Harris KM, Cruce WL, Greenough WT, Teyler TJ. A Golgi impregnation technique for thin brain slices maintained in vitro. J Neurosci Methods. 1980 Aug;2(4):363-71. Spacek J. Dynamics of the Golgi method: a time-lapse study of the early stages of impregnation in single sections. J Neurocytol. 1989 Feb;18(1):27-38. Moss TL, Whetsell WO. Techniques for thick-section Golgi impregnation of formalin-fixed brain tissue. Methods Mol Biol. 2004;277:277-85. I don't remember if any of these discuss re-staining, but if you don't have any silver chromate crystals in the specimens it probably can't hurt to return them to the chromation step. Tricky business getting consistent staining on sections in any case. You might still be able to use a Cox alternative. I've had good luck with Gibb and Kolb's method on some thick sections although it was described for whole brain (Gibb R, Kolb B. A method for vibratome sectioning of Golgi-Cox stained whole rat brain. J Neurosci Methods. 1998 Jan 31;79(1):1-4.). Good luck, Mike King UF Pharmacology & Therapeutics ------------------ Message: 20 Date: Tue, 04 Aug 2009 17:51:17 +0200 From: "Dr. Frauke Neff" Subject: [Histonet] golgi stain- please help To: histonet@lists.utsouthwestern.edu Message-ID: <20090804175117.z828f2xw3oow80sg@home.staff.uni-marburg.de> Content-Type: text/plain; charset=ISO-8859-1; DelSp="Yes"; format="flowed" Dear Histonetters, I was performing a golgi stain on mouse brains and my treated animal group gave nice results but the untreated control group did not show any staining at all (it looks like the staining didn't work). Does anyone know / have an idea, if I could repeat the staining on these vibratome sections? I read something about "deimpregnation" of golgi stained slides, but found no protocol and I'm not sure if I can perform another "fresh" golgi stain with these slides. Any suggestion is welcome!!! Thanks to all of you, Frauke From carl.hobbs <@t> kcl.ac.uk Tue Aug 4 13:23:24 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Tue Aug 4 13:23:59 2009 Subject: [Histonet] Re: reagents for IHC Message-ID: <11D9615B89C10747B1C985966A63D7CA2991742985@KCL-MAIL04.kclad.ds.kcl.ac.uk> Are you using automated IHC-staining machinery, Naira? From rgrow <@t> bmnet.com Tue Aug 4 13:48:24 2009 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Tue Aug 4 13:48:31 2009 Subject: [Histonet] Re: WindoPath In-Reply-To: Message-ID: Chisti, We've used WindoPath for the last 8 years. Good AP system. Good archives, can make modifications to suit your institution, etc. The only negative that I can give you is: Stay far, FAR away from the 6.0 version. We are using it, but can't wait for the hospital to approve the budget for another upgrade. Our 5.0 version was more stable/user friendly. But we were forced to upgrade as they were no longer supporting 5.0. I can give you further details if needed. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax Message: 3 Date: Mon, 3 Aug 2009 11:39:02 -0700 (PDT) From: Cristi stephenson Subject: [Histonet] WindoPath To: histonet@lists.utsouthwestern.edu Message-ID: <428592.32791.qm@web81202.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all, Does anyone out there currently use WindoPath as their LIS?? Any opinions or pointers concerning set-up, usage and the like? Thanks in advance?for your guidance, Cristi Stephenson MSA, HT(ASCP) ------------------------------ From brett_connolly <@t> merck.com Tue Aug 4 13:50:58 2009 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Aug 4 13:51:16 2009 Subject: [Histonet] RA Lamb paraffin Message-ID: <63EA0607835FBA4689CEA9EA8B482692022F8DBC@usctmx1141.merck.com> One of my colleagues has been using paraffin from Raymond A Lamb for embedding rodent brains. She loves it, but now that ThermoFisher has acquired RA Lamb this paraffin is being discontinued. Does anyone know of any other distributors that might still have some stock...or an equivalent substitute paraffin? Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From brian <@t> prometheushealthcare.com Tue Aug 4 15:33:03 2009 From: brian <@t> prometheushealthcare.com (Brian with Prometheus) Date: Tue Aug 4 14:31:34 2009 Subject: [Histonet] Laboratory Openings in New York Area Message-ID: <446fa14008cd466c815c21e33f653a38@prometheushealthcare.com> Hello, Below are a few of the positions we are currently working on in NY. We are offering up to $1,000 for referrals if we place qualified candidates in these openings. These positions vary from Histology to Medical Technologist positions. Any help would be appreciated. I?ve listed the positions below.   Special Chemistry Supervisor in Elmwood, NJ. It is a day shift with competitive pay. The candidate must have hplc testing experience.   Histotech in Rye Brook, Port Chester, New Rochelle, Uniondale, Yonkers, and others in the area. Shifts as well as pay vary as each location is different. Must have Clinical Laboratory Technologist New York licensure.   Blood Bank Medical Technologist, in West Chester, NY. The candidate must have New York licensure and experience in a blood bank. Also the position is a night shift.   Blood Bank Supervisor in West Chester, NY. Candidate must have New York Licensure and supervisory experience. The position is a night shift.   Generalist Lab Manager in Manhattan, NY with a small stat lab. The candidate must have NY Laboratory Supervisor license. They are looking for someone who has supervisory experience with a lab.   Micro and Hematology Medical Technologist with a reference laboratory in Syosset. The shift is currently not available because it is a new position. The candidate must have their Clinical Laboratory Technologist license in NY.   Thanks again, and any help is appreciated!!!!!!! Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com *** Stay up to date on the newest positions and healthcare trends nationwide on Twitter!***  http://twitter.com/PrometheusBlog       From Kris.Caldwell <@t> leica-microsystems.com Tue Aug 4 14:56:50 2009 From: Kris.Caldwell <@t> leica-microsystems.com (Kris.Caldwell@leica-microsystems.com) Date: Tue Aug 4 14:56:52 2009 Subject: [Histonet] Several new opportunities with Leica Microsystems Message-ID: Leica Microsystems is a leading global designer and producer of innovative high-tech precision optics systems for the analysis of microstructures. It is one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. Due to growth we are currently hiring the following positions: Bond Reagents Marketing Manager: - Chicago, IL IHC Sales Specialist - West, Mid-Atlantic, N.TX/OK/AR, and Southeast Field Support Specialist - West Sales Account Executive - West Field Service Engineer - TN Regional Service Manager - Canada Leica offers competitive salary, benefits including medical, dental, vision, prescription, long-term care, life insurance, STD, LTD, and 401 (k). Please forward your resume to: kris.caldwell@leica-microsystems.com Kris Caldwell Human Resources Recruiter Leica Microsystems, Inc. 2345 Waukegan Road Bannockburn, IL 60015 www.leica-microsystems.com Kris.Caldwell@Leica-Microsystems.com 847-405-5432 - phone 847-236-3035 - fax 847-323-6169- cellular ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From pnagappan <@t> cau.edu Tue Aug 4 15:17:54 2009 From: pnagappan <@t> cau.edu (Nagappan, Peri) Date: Tue Aug 4 15:15:31 2009 Subject: [Histonet] Paraffin Sections Message-ID: <0BB96939ADC2BC4881B7ACC890BE6FC1091BC8@cauexch01.cau.edu> Hi Histonetters, When I cut the paraffin sections in the microtome, I am not getting the whole sections intact, rather some portion in the middle of the sections are brittle. But the paraffin portion surrounds the tissue is smooth, nice and intact. Thanks for your suggestion and help. Peri pnagappan@cau.edu From kgrobert <@t> rci.rutgers.edu Tue Aug 4 15:40:20 2009 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Tue Aug 4 15:26:05 2009 Subject: [Histonet] Paraffin Sections In-Reply-To: <0BB96939ADC2BC4881B7ACC890BE6FC1091BC8@cauexch01.cau.edu> References: <0BB96939ADC2BC4881B7ACC890BE6FC1091BC8@cauexch01.cau.edu> Message-ID: <4A789CB4.1060200@rci.rutgers.edu> Peri, Sounds like poor infiltration to me. What kind of tissue are we talking about, and what processing program did you use? Kathleen Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854 Nagappan, Peri wrote: >Hi Histonetters, > > > >When I cut the paraffin sections in the microtome, I am not getting the whole sections intact, rather some portion in the middle of the sections are brittle. But the paraffin portion surrounds the tissue is smooth, nice and intact. > > > >Thanks for your suggestion and help. > > > >Peri > >pnagappan@cau.edu > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From kblack <@t> digestivehlth.com Tue Aug 4 15:37:47 2009 From: kblack <@t> digestivehlth.com (Konni Black) Date: Tue Aug 4 15:37:55 2009 Subject: [Histonet] (no subject) Message-ID: <49C8A4A728234A91802314B1CA19A586@digestivehlth.com> Part-time job opening for an experienced HT or HTL in St. Augustine, Florida. It is a new lab for a group of gastroenterologists. I estimate it will require 4 to 6 hours per day. Very flexible hours; design your own schedule. Pay is commensurate with experience and local scale. Great working conditions. Could be a great second job or an enjoyable first job. Must have 3 to 5 years experience. Probable opening in September 2009. Konni Black Pathology/Lab Development, LLC 253-503-2560 From igor.deyneko <@t> gmail.com Tue Aug 4 15:43:08 2009 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Tue Aug 4 15:43:12 2009 Subject: [Histonet] Tissue Processor Advice Message-ID: <35e16a770908041343t1bc0ccd1i749e3d18f1c77d8c@mail.gmail.com> Dear Histonetters! I need your advice in Tissue Processors. The one we currently use, Tissue Tek VIP 3000, is archaic and has finally died. So we are looking into buying a new one. I know that Thermo and Leica both have processors, as well as new Tissue teks, but I wanted to get opinions if you have a preference of a machine, pros and cons of each. I mostly process tumors, with occasional mouse organs thrown in. Any suggestions will be very helpful! Thank you in advance. Sincerely, Igor Deyneko Infinity Pharmaceuticals Cambridge, MA From TGoins <@t> mt.gov Tue Aug 4 16:11:48 2009 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Tue Aug 4 16:11:53 2009 Subject: [Histonet] Eosin Bleeding Message-ID: <764D82B61A7B8141AC0659805358A21A133F06E037@doaisd05220.state.mt.ads> We very occasionally have a single slide out of a group of 100 or more where the eosin will bleed after coverslipping. Does anyone know if this is associated with a particular tissue type or treatment with Nair to soften keratin? Thanks, Tresa Veterinary Diagnostic Lab Bozeman, MT From geoweigel <@t> hotmail.com Tue Aug 4 16:23:22 2009 From: geoweigel <@t> hotmail.com (Georgia Weigel) Date: Tue Aug 4 16:23:25 2009 Subject: [Histonet] Hi folks, looking for contact information for a lo... Message-ID: Hi folks, looking for contact information for a long lost histotech colleague. Looking for a dear friend named Fran Lemons or Fran Walker. Last known to be working in Tennessee. Feel free to contact me with a private e-mail. geoweigel@hotmail.com Thank you all, Georgia Weigel, HT(ASCP) 361-960-3886 _________________________________________________________________ Windows Live?: Keep your life in sync. http://windowslive.com/explore?ocid=PID23384::T:WLMTAGL:ON:WL:en-US:NF_BR_sync:082009 From thecitan <@t> yahoo.com Tue Aug 4 17:09:22 2009 From: thecitan <@t> yahoo.com (thecitan@yahoo.com) Date: Tue Aug 4 17:08:00 2009 Subject: [Histonet] Paraffin Sections Message-ID: <734295547-1249423675-cardhu_decombobulator_blackberry.rim.net-259360817-@bxe1123.bisx.prod.on.blackberry> You running particularly small specimens like derm? You may need to decrease time in alcohol. Adding a little ammonia water to your ice bath and soaking after facing the block may help too. ------Original Message------ From: Kathleen Roberts Sender: histonet-bounces@lists.utsouthwestern.edu To: Nagappan, Peri Cc: Histonet Subject: Re: [Histonet] Paraffin Sections Sent: Aug 4, 2009 1:40 PM Peri, Sounds like poor infiltration to me. What kind of tissue are we talking about, and what processing program did you use? Kathleen Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854 Nagappan, Peri wrote: >Hi Histonetters, > > > >When I cut the paraffin sections in the microtome, I am not getting the whole sections intact, rather some portion in the middle of the sections are brittle. But the paraffin portion surrounds the tissue is smooth, nice and intact. > > > >Thanks for your suggestion and help. > > > >Peri > >pnagappan@cau.edu > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent from my Verizon Wireless BlackBerry From thecitan <@t> yahoo.com Tue Aug 4 17:45:35 2009 From: thecitan <@t> yahoo.com (thecitan@yahoo.com) Date: Tue Aug 4 17:44:08 2009 Subject: [Histonet] Tissue Processor Advice Message-ID: <1624411673-1249425843-cardhu_decombobulator_blackberry.rim.net-1290136412-@bxe1123.bisx.prod.on.blackberry> I recommend the new tissue tech. I use it in my lab and its reliable and intuitive. I strongly warn against TBS. I use one in my other lab and its been nothing but trouble. Its chemical storage is unreliable and leads to cross contamination. ------Original Message------ From: Igor Deyneko Sender: histonet-bounces@lists.utsouthwestern.edu To: Histonet Subject: [Histonet] Tissue Processor Advice Sent: Aug 4, 2009 1:43 PM Dear Histonetters! I need your advice in Tissue Processors. The one we currently use, Tissue Tek VIP 3000, is archaic and has finally died. So we are looking into buying a new one. I know that Thermo and Leica both have processors, as well as new Tissue teks, but I wanted to get opinions if you have a preference of a machine, pros and cons of each. I mostly process tumors, with occasional mouse organs thrown in. Any suggestions will be very helpful! Thank you in advance. Sincerely, Igor Deyneko Infinity Pharmaceuticals Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent from my Verizon Wireless BlackBerry From macveigh <@t> usc.edu Tue Aug 4 18:20:53 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Tue Aug 4 18:20:56 2009 Subject: [Histonet] working with mouse liver... Message-ID: <001e01ca155a$365bdb70$5c237d80@DFS66DD1> Hi all, We have a small liver core and we are having problem with the look/morphology of the liver. It looks like large part of the cytoplasm of the liver cells is missing. The normal mice are the worst. (It is not fat that is dissolved.) We use commercially made 10% NBF and tried fixing for different length of time with no luck. The processing time is 1 hour in every station. Did anybody working with liver has any suggestions? Michelle From stamptrain <@t> yahoo.com Tue Aug 4 18:33:33 2009 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Tue Aug 4 18:33:36 2009 Subject: [Histonet] working with mouse liver... In-Reply-To: <001e01ca155a$365bdb70$5c237d80@DFS66DD1> References: <001e01ca155a$365bdb70$5c237d80@DFS66DD1> Message-ID: <691708.47890.qm@web55802.mail.re3.yahoo.com> In my experience you are way over processing the tissue.? Drop your processing times to about 30 min per station.? That should be fine. Roger Moretz, Ph.D. (ret) ----- Original Message ---- From: Michelle MacVeigh-Aloni To: Histonet@lists.utsouthwestern.edu Sent: Tuesday, August 4, 2009 7:20:53 PM Subject: [Histonet] working with mouse liver... Hi all, We have a small liver core and we are having problem with the look/morphology of the liver. It looks like large part of the cytoplasm of the liver cells is missing. The normal mice are the worst. (It is not fat that is dissolved.) We use commercially made 10% NBF and tried fixing for different length of time with no luck. The processing time is 1 hour in every station. Did anybody working with liver has any suggestions? Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annigyg <@t> gmail.com Wed Aug 5 02:43:34 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed Aug 5 02:43:48 2009 Subject: [Histonet] Tissue Processor Advice In-Reply-To: <35e16a770908041343t1bc0ccd1i749e3d18f1c77d8c@mail.gmail.com> References: <35e16a770908041343t1bc0ccd1i749e3d18f1c77d8c@mail.gmail.com> Message-ID: Sakura Tissue Tek VIP5!! I have 2 of these sturdy workhorses and am very happy Have been associated with VIP5's for as long as they have been around - wonderful machines! AnnieinAbuDhabi aka.Annie out of Africa 2009/8/5 Igor Deyneko > Dear Histonetters! > I need your advice in Tissue Processors. The one we currently use, Tissue > Tek VIP 3000, is archaic and has finally died. So we are looking into > buying > a new one. I know that Thermo and Leica both have processors, as well as > new > Tissue teks, but I wanted to get opinions if you have a preference of a > machine, pros and cons of each. I mostly process tumors, with occasional > mouse organs thrown in. > Any suggestions will be very helpful! > Thank you in advance. > Sincerely, > Igor Deyneko > Infinity Pharmaceuticals > Cambridge, MA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From billodonnell <@t> catholichealth.net Wed Aug 5 08:11:49 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Aug 5 08:12:03 2009 Subject: [Histonet] Clear-Rite 3 Message-ID: Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all "Xylene Substitutes" pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 From HornHV <@t> archildrens.org Wed Aug 5 08:52:47 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Aug 5 08:52:51 2009 Subject: [Histonet] HM 355 S microtome Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D8338A@EMAIL.archildrens.org> If there are any vendors who sell this microtome besides thermofisher would you contact me offlist? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From portera <@t> msu.edu Wed Aug 5 09:00:47 2009 From: portera <@t> msu.edu (Amy Porter) Date: Wed Aug 5 09:00:53 2009 Subject: [Histonet] Clear-Rite 3 References: Message-ID: <0A863BA5E09C4AEC802E89F07377A58C@histolab> I would contact Anatech Ltd. they have a substitute "Pro-Par Clearant" www.anatechltdusa.com - they are all very knowledgeable and would be able to help you I am sure. I don't use substitutes in my lab at this time, however I would contact them if I were ready to make the switch. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "O'Donnell, Bill" To: Sent: Wednesday, August 05, 2009 9:11 AM Subject: [Histonet] Clear-Rite 3 Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all "Xylene Substitutes" pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Aug 5 09:01:22 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 5 09:01:27 2009 Subject: [Histonet] Clear-Rite 3 In-Reply-To: Message-ID: <936052.17397.qm@web65712.mail.ac4.yahoo.com> Bill: Xylene substitutes are not all?"pretty much the same". There are d-Limonene derivatives (something to stay absolutely away from) and alkane derivatives with many known and unknown constituents. Since you are about to change and not have any preference, your best option both in processing quality and price, would be to get a reliable supplier of "mineral spirits" (the same you can find at any home improvement store) and use it. Ren? J.? --- On Wed, 8/5/09, O'Donnell, Bill wrote: From: O'Donnell, Bill Subject: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu Date: Wednesday, August 5, 2009, 9:11 AM Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all "Xylene Substitutes" pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed Aug 5 09:14:38 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Aug 5 09:11:56 2009 Subject: [Histonet] RE: Clear-Rite 3 In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E390894C787B8@IBMB7Exchange.digestivespecialists.com> Bill, I have used Formula 83 from CBG Biotech for several years now and like it. There is no strong odor, dries fast and is recyclable. http://www.cbgbiotech.com Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, August 05, 2009 9:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Clear-Rite 3 Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all "Xylene Substitutes" pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Wed Aug 5 09:15:22 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Wed Aug 5 09:15:25 2009 Subject: [Histonet] Clear-Rite 3 In-Reply-To: <936052.17397.qm@web65712.mail.ac4.yahoo.com> References: <936052.17397.qm@web65712.mail.ac4.yahoo.com> Message-ID: <003701ca15d7$2bc96010$835c2030$@net> Sorry that is incorrect as companies also offer a substitute that is an isopar derivative that is not d-limonene and has almost no order. I have used for several years and is very good. A number of suppliers offer it. The isopar product is a petroleum distillate and is a known product although the companies call it a non-aliphatic hydrocarbon. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, August 05, 2009 10:01 AM To: Histonet@lists.utsouthwestern.edu; BillO'Donnell Subject: Re: [Histonet] Clear-Rite 3 Bill: Xylene substitutes are not all?"pretty much the same". There are d-Limonene derivatives (something to stay absolutely away from) and alkane derivatives with many known and unknown constituents. Since you are about to change and not have any preference, your best option both in processing quality and price, would be to get a reliable supplier of "mineral spirits" (the same you can find at any home improvement store) and use it. Ren? J.? --- On Wed, 8/5/09, O'Donnell, Bill wrote: From: O'Donnell, Bill Subject: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu Date: Wednesday, August 5, 2009, 9:11 AM Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all "Xylene Substitutes" pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katemaddox <@t> yahoo.com Wed Aug 5 09:41:49 2009 From: katemaddox <@t> yahoo.com (Kathryn Maddox) Date: Wed Aug 5 09:41:52 2009 Subject: [Histonet] Helicobacter control slides Message-ID: <697053.38529.qm@web34706.mail.mud.yahoo.com> Hi, ?? I need to buy some Helicobacter Pylori special stain control slides. The ones I have previously bought were from rat tissue and our pathologists don't like them. Can anyone suggest a company that I can purchase them from? Thanks! ? Kathy Maddox Lake Charles Memorial Hospital Lake Charles, LA From JCBRITTON <@t> Cheshire-Med.COM Wed Aug 5 10:27:42 2009 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Wed Aug 5 10:27:52 2009 Subject: [Histonet] Helicobacter control slides In-Reply-To: <697053.38529.qm@web34706.mail.mud.yahoo.com> References: <697053.38529.qm@web34706.mail.mud.yahoo.com> Message-ID: www.americanmastsertech.com , Item: CSHEL25, Helicobacter Pylori control slides. Josie Britton HT Cheshire Medical Center Keene, NH ----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathryn Maddox Sent: Wednesday, August 05, 2009 10:42 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter control slides Hi, I need to buy some Helicobacter Pylori special stain control slides. The ones I have previously bought were from rat tissue and our pathologists don't like them. Can anyone suggest a company that I can purchase them from? Thanks! Kathy Maddox Lake Charles Memorial Hospital Lake Charles, LA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From TJJ <@t> stowers.org Wed Aug 5 10:50:33 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Wed Aug 5 10:51:37 2009 Subject: [Histonet] Re: working with mouse liver... Message-ID: Michelle, Could it be what you are seeing is normal healthy (actively feeding) liver in mice and the missing cytoplasm is where the glycogen stores are/were? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From Heather.D.Renko <@t> osfhealthcare.org Wed Aug 5 11:03:47 2009 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Wed Aug 5 11:04:01 2009 Subject: [Histonet] re: incomplete sections Message-ID: From personal experience some possible solutions: * Infiltration issues ( is your paraffin fresh, carry over on reagents) * Blocks being too warm or too wet * Once had this issue when cleaning of the processor wasn't being properly followed. Hope this helps a bit. Heather D. Renko, Histology Supervisor OSF Saint Anthony Medical Center Main Laboratory-Histology 5666 East State Street Rockford, Illinois 61108 815-395-5410 Direct 815-395-5116 Department ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From jcline <@t> wchsys.org Wed Aug 5 11:45:20 2009 From: jcline <@t> wchsys.org (Joyce Cline) Date: Wed Aug 5 11:45:28 2009 Subject: [Histonet] Clear-Rite 3 In-Reply-To: Message-ID: <80C5A0CDEF3B45EFA0EEC676FAD52050@wchsys.org> I use Formula 83 from CBG. I used Clear-Rite for quite a few years, but Formula 83 works as close to Xylene as I have found. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, August 05, 2009 9:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Clear-Rite 3 Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all "Xylene Substitutes" pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From vapatpxs <@t> yahoo.com Wed Aug 5 11:48:59 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Wed Aug 5 11:49:02 2009 Subject: [Histonet] Clear-Rite 3 In-Reply-To: <936052.17397.qm@web65712.mail.ac4.yahoo.com> Message-ID: <540060.13022.qm@web46103.mail.sp1.yahoo.com> Hi Rene, Why do you not like the d-limonene solvents? I work with them and the results seem fine.? I have to mention that I use an AutoTechnicon Duo and cannot place it in a fume hood to vent the xylene vapors.? Without proper ventilation the safety people willnot allow the use of Xylenen.? I work in the research unit of a VA hospital and they will not provide larger fume hoods nor buy me a self contained processing sytem. Please let me know what other safe alternatives (ones that can be used out on the bench) are you suggest. Thanks, Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Wed, 8/5/09, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu, "BillO'Donnell" Date: Wednesday, August 5, 2009, 2:01 PM Bill: Xylene substitutes are not all?"pretty much the same". There are d-Limonene derivatives (something to stay absolutely away from) and alkane derivatives with many known and unknown constituents. Since you are about to change and not have any preference, your best option both in processing quality and price, would be to get a reliable supplier of "mineral spirits" (the same you can find at any home improvement store) and use it. Ren? J.? --- On Wed, 8/5/09, O'Donnell, Bill wrote: From: O'Donnell, Bill Subject: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu Date: Wednesday, August 5, 2009, 9:11 AM Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all "Xylene Substitutes" pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSHERWOOD <@t> PARTNERS.ORG Wed Aug 5 12:23:44 2009 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Aug 5 12:23:47 2009 Subject: [Histonet] Clear-Rite 3 In-Reply-To: <540060.13022.qm@web46103.mail.sp1.yahoo.com> References: <936052.17397.qm@web65712.mail.ac4.yahoo.com> <540060.13022.qm@web46103.mail.sp1.yahoo.com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23BEA@PHSXMB30.partners.org> We also use d-limonene (sold as CitriSolv). We used Hemo-De before. I also would be curious you (and others) do not like d-limonene. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello Sent: Wednesday, August 05, 2009 12:49 PM To: Rene J Buesa; HistoNet Subject: Re: [Histonet] Clear-Rite 3 Hi Rene, Why do you not like the d-limonene solvents? I work with them and the results seem fine.? I have to mention that I use an AutoTechnicon Duo and cannot place it in a fume hood to vent the xylene vapors.? Without proper ventilation the safety people willnot allow the use of Xylenen.? I work in the research unit of a VA hospital and they will not provide larger fume hoods nor buy me a self contained processing sytem. Please let me know what other safe alternatives (ones that can be used out on the bench) are you suggest. Thanks, Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Wed, 8/5/09, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu, "BillO'Donnell" Date: Wednesday, August 5, 2009, 2:01 PM Bill: Xylene substitutes are not all?"pretty much the same". There are d-Limonene derivatives (something to stay absolutely away from) and alkane derivatives with many known and unknown constituents. Since you are about to change and not have any preference, your best option both in processing quality and price, would be to get a reliable supplier of "mineral spirits" (the same you can find at any home improvement store) and use it. Ren? J.? --- On Wed, 8/5/09, O'Donnell, Bill wrote: From: O'Donnell, Bill Subject: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu Date: Wednesday, August 5, 2009, 9:11 AM Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all "Xylene Substitutes" pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From victor <@t> pathology.washington.edu Wed Aug 5 12:44:31 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Aug 5 12:44:36 2009 Subject: [Histonet] Clear-Rite 3 In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23BEA@PHSXMB30.partners.org> References: <936052.17397.qm@web65712.mail.ac4.yahoo.com> <540060.13022.qm@web46103.mail.sp1.yahoo.com> <073AE2BEA1C2BA4A8837AB6C4B943D9703E23BEA@PHSXMB30.partners.org> Message-ID: <4A79C4FF.1080609@pathology.washington.edu> The last time I was around a citrus based clearing agent was in the 80's and it gave me severe headaches. I didn't have any problems with it's performance, just the health aspects. I was using a closed processor at the time, but no hood.. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Sherwood, Margaret wrote: > We also use d-limonene (sold as CitriSolv). We used Hemo-De before. I also > would be curious you (and others) do not like d-limonene. > > Peggy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Va Paula > Sicurello > Sent: Wednesday, August 05, 2009 12:49 PM > To: Rene J Buesa; HistoNet > Subject: Re: [Histonet] Clear-Rite 3 > > Hi Rene, > > Why do you not like the d-limonene solvents? > > I work with them and the results seem fine. I have to mention that I use an > AutoTechnicon Duo and cannot place it in a fume hood to vent the xylene vapors. > Without proper ventilation the safety people willnot allow the use of Xylenen. > I work in the research unit of a VA hospital and they will not provide larger > fume hoods nor buy me a self contained processing sytem. > > Please let me know what other safe alternatives (ones that can be used out on > the bench) are you suggest. > > Thanks, > > Paula Sicurello > > VA Medical Center San Diego > > Veterans Medical Research Foundation (VMRF) > > Core for Micro Imaging(C-MI) > > 3350 La Jolla Village Dr., MC151 > > San Diego, CA 92161 > > 858-552-8585 x2397 > > > > C-MI for your imaging needs. > > --- On Wed, 8/5/09, Rene J Buesa wrote: > > From: Rene J Buesa > Subject: Re: [Histonet] Clear-Rite 3 > To: Histonet@lists.utsouthwestern.edu, "BillO'Donnell" > > Date: Wednesday, August 5, 2009, 2:01 PM > > Bill: > Xylene substitutes are not all "pretty much the same". There are d-Limonene > derivatives (something to stay absolutely away from) and alkane derivatives with > many known and unknown constituents. > Since you are about to change and not have any preference, your best option both > in processing quality and price, would be to get a reliable supplier of "mineral > spirits" (the same you can find at any home improvement store) and use it. > Ren? J. > > --- On Wed, 8/5/09, O'Donnell, Bill wrote: > > > From: O'Donnell, Bill > Subject: [Histonet] Clear-Rite 3 > To: Histonet@lists.utsouthwestern.edu > Date: Wednesday, August 5, 2009, 9:11 AM > > > Greetings! > > We have been using Clear-Rite 3 here at our lab, and we are happy with > the product. Our supplier says it will be on back-order for some time > now. Our crack supply folks are looking for another source. I'm taking > another route to find out what products out there are comparable. > > Are all "Xylene Substitutes" pretty much the same and there for pretty > much interchangable? > Are there some to stay away from? > > Any help is appriciated. > > William (Bill) O'Donnell, HT (ASCP) QIHC > Lead Histologist > Good Samaritan Hospital > 10 East 31st Street > Kearney, NE 68847 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From zippedinsunshine <@t> aol.com Wed Aug 5 13:08:58 2009 From: zippedinsunshine <@t> aol.com (zippedinsunshine@aol.com) Date: Wed Aug 5 13:09:20 2009 Subject: [Histonet] please remove me from list Message-ID: <8CBE40EF02DA91F-1804-526@WEBMAIL-MZ29.sysops.aol.com> From lchung <@t> ppmh.org Wed Aug 5 13:21:30 2009 From: lchung <@t> ppmh.org (Chung, Luong) Date: Wed Aug 5 13:21:50 2009 Subject: [Histonet] please remove me from list In-Reply-To: <8CBE40EF02DA91F-1804-526@WEBMAIL-MZ29.sysops.aol.com> Message-ID: <86691924ECCDBE4F82CCAB553424607101DC6A4D@exchange2.phoebe.com> Unsubscribe me Please. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of zippedinsunshine@aol.com Sent: Wednesday, August 05, 2009 2:09 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] please remove me from list _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Disclaimer: The HIPAA Final Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. 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From Brenda <@t> nsh.org Wed Aug 5 14:08:54 2009 From: Brenda <@t> nsh.org (Brenda Royce) Date: Wed Aug 5 14:08:58 2009 Subject: [Histonet] NSH 35th Annual Symposium/Convention Message-ID: The NSH S/C Provides Incredible Opportunities to Network >From discussions with fellow attendees, NSH sponsored events and spectacular evening events the 2009 S/C has a little something for everyone. Evening Hospitalities The Annual Scientific Exhibit Show is a large part of the S/C experience. Our vendor partners bring the best the industry has to offer during the day & sponsor great opportunities for fun at night. Keep your eyes open for invitations to industry parties and dinners. National Society for Histotechnology Annual Awards Banquet The Annual NSH Awards Banquet is a celebration of the best in histology. The NSH Awards committee works with vendor partners to handout out over $30,000 in awards and scholarships. It's also at this event that NSH will announce the 2009 Histotech of the Year. . The evening includes a cocktail reception (sponsored by Sakura Finetek), Dinner & Dancing. Tickets are $35.00 (nonrefundable). Dress is Semi Formal. First Time Attendee Welcome Reception Before you explore Birmingham we invite first time S/C attendees to join us for a unique welcome to the NSH Community Friday, October 2 at 6:45pm. Hors d'oeuvres and beverages will be served. The event concludes with prize drawings. Annual T Shirt Contest A perennial favorite at the S/C - state societies where a T Shirt designed by their members for the EMD Chemical sponsored T Shirt Contest on Tuesday afternoon. The winning T Shirts earn prize money for their local societies. Contact your local society & join us in showing your community spirit & pride in histology. Register Now! Online Download Brochure From rjbuesa <@t> yahoo.com Wed Aug 5 14:25:01 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 5 14:25:10 2009 Subject: [Histonet] Clear-Rite 3 In-Reply-To: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23BEA@PHSXMB30.partners.org> Message-ID: <253124.96922.qm@web65705.mail.ac4.yahoo.com> My answer is in the attached article I just published. Please read the d-Limonene discussion seciton. Ren? J. --- On Wed, 8/5/09, Sherwood, Margaret wrote: From: Sherwood, Margaret Subject: RE: [Histonet] Clear-Rite 3 To: "Va Paula Sicurello" , "Rene J Buesa" , "HistoNet" Date: Wednesday, August 5, 2009, 1:23 PM We also use d-limonene (sold as CitriSolv).? We used Hemo-De before.? I also would be curious you (and others) do not like d-limonene. Peggy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello Sent: Wednesday, August 05, 2009 12:49 PM To: Rene J Buesa; HistoNet Subject: Re: [Histonet] Clear-Rite 3 Hi Rene, Why do you not like the d-limonene solvents? I work with them and the results seem fine.? I have to mention that I use an AutoTechnicon Duo and cannot place it in a fume hood to vent the xylene vapors.? Without proper ventilation the safety people willnot allow the use of Xylenen.? I work in the research unit of a VA hospital and they will not provide larger fume hoods nor buy me a self contained processing sytem. Please let me know what other safe alternatives (ones that can be used out on the bench) are you suggest. Thanks, Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Wed, 8/5/09, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu, "BillO'Donnell" Date: Wednesday, August 5, 2009, 2:01 PM Bill: Xylene substitutes are not all?"pretty much the same". There are d-Limonene derivatives (something to stay absolutely away from) and alkane derivatives with many known and unknown constituents. Since you are about to change and not have any preference, your best option both in processing quality and price, would be to get a reliable supplier of "mineral spirits" (the same you can find at any home improvement store) and use it. Ren? J.? --- On Wed, 8/5/09, O'Donnell, Bill wrote: From: O'Donnell, Bill Subject: [Histonet] Clear-Rite 3 To: Histonet@lists.utsouthwestern.edu Date: Wednesday, August 5, 2009, 9:11 AM Greetings! We have been using Clear-Rite 3 here at our lab, and we are happy with the product. Our supplier says it will be on back-order for some time now. Our crack supply folks are looking for another source. I'm taking another route to find out what products out there are comparable. Are all "Xylene Substitutes" pretty much the same and there for pretty much interchangable? Are there some to stay away from? Any help is appriciated. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From rsrichmond <@t> gmail.com Wed Aug 5 14:50:56 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Aug 5 14:51:01 2009 Subject: [Histonet] Re: Helicobacter control slides Message-ID: Kathy Maddox in Lake Charles, Louisiana asks: >>I need to buy some Helicobacter Pylori special stain control slides. The ones I have previously bought were from rat tissue and our pathologists don't like them. Can anyone suggest a company that I can purchase them from?<< Most of the pathology services I've worked with use known positives from their own cases. (Once in a great while you'll see a gastrectomy specimen that provides a lifetime supply.) Obviously the material should be positive with the stain you use (dye? silver? IHC?) I'd trust animal tissue for a dye stain, definitely not for an immunostain, since there are many species of Helicobacter. The two species that infect humans, H. pylori and H. heilmannii )= Gastrospirillum hominis) both mark with the standard IHC reagents. Bob Richmond Samurai Pathologist Knoxville TN From rsrichmond <@t> gmail.com Wed Aug 5 15:02:44 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Wed Aug 5 15:02:48 2009 Subject: [Histonet] Re: Xylene substitutes Message-ID: William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good Samaritan Hospital, Kearney, Nebraska, notes that Clear-Rite 3 is at least temporarily unavailable, and he is looking for a substitute. (Anybody know what's going on with Clear-Rite 3?) Clear-Rite 3 is an aliphatic (rather than a limonene). Aliphatics can't always be substituted for each other - they are chemically different from each other - each has its own flammability characteristics (flash point) and (if you're recovering solvent using a spinning band still) its own distillation routine. If you change, you may want to stay with the product you change to. Your purchasing people need to be aware that they are not to change brands on you. I just worked in a lab that has a Leica ASP300S processor (which they like, by the way). The people there have been told that they may use only one particular aliphatic with it, one I hadn't heard of before: Sub-X aliphatic hydrocarbon isoparaffinic oil CAS 6472-48-9 DOT petroleum distillates, NOS, 3 class 3 flammable liquid Flash point 106 F (41 C) Anybody know if this is actually the case? Bob Richmond Samurai Pathologist Knoxville TN From diane.gladney <@t> us.army.mil Wed Aug 5 15:12:24 2009 From: diane.gladney <@t> us.army.mil (Gladney, Diane C Ms CIV USA MEDCOM MACH) Date: Wed Aug 5 15:12:41 2009 Subject: [Histonet] Re: Xylene substitutes (UNCLASSIFIED) In-Reply-To: References: Message-ID: <6673D9F600F29943A80ACD92795D34504F2239@amedsermcbe042.amed.ds.army.mil> Classification: UNCLASSIFIED Caveats: NONE I have 2 Leica ASP 300 tissue processors. I use Thermo Scientific Shandon Xylene Substitute (formally known as Histosolv). I have used this for many years with excellent results. I have never had any problems using this in the processor. We had 2 very old VIP tissue processors before we got the Leica processors and used Histosolv in those processors also. I have never heard of Sub-X. Diane C. Gladney, HT (ASCP) Supervisor, Anatomical Pathology Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC? 29207 Email:? diane.gladney@amedd.army.mil Phone:? 803-751- 2530 FAX:???? 803-751-7829 DSN:??? 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, August 05, 2009 4:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Xylene substitutes William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good Samaritan Hospital, Kearney, Nebraska, notes that Clear-Rite 3 is at least temporarily unavailable, and he is looking for a substitute. (Anybody know what's going on with Clear-Rite 3?) Clear-Rite 3 is an aliphatic (rather than a limonene). Aliphatics can't always be substituted for each other - they are chemically different from each other - each has its own flammability characteristics (flash point) and (if you're recovering solvent using a spinning band still) its own distillation routine. If you change, you may want to stay with the product you change to. Your purchasing people need to be aware that they are not to change brands on you. I just worked in a lab that has a Leica ASP300S processor (which they like, by the way). The people there have been told that they may use only one particular aliphatic with it, one I hadn't heard of before: Sub-X aliphatic hydrocarbon isoparaffinic oil CAS 6472-48-9 DOT petroleum distillates, NOS, 3 class 3 flammable liquid Flash point 106 F (41 C) Anybody know if this is actually the case? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE From PMonfils <@t> Lifespan.org Wed Aug 5 15:46:03 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Aug 5 15:46:07 2009 Subject: [Histonet] Eosin Bleeding In-Reply-To: <764D82B61A7B8141AC0659805358A21A133F06E037@doaisd05220.state.mt.ads> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CD8@LSRIEXCH1.lsmaster.lifespan.org> Can't say for sure but eosin is very sensitive to pH, and will rinse out rapidly in any solution that is basic, even slightly so, like tap water. Nair contains calcium hydroxide, which is a stong base. If that compound were not thoroughly rinsed out of the tissue before processing, I wouldn't be surprised if it interfered with eosin binding. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Goins, Tresa > Sent: Tuesday, August 4, 2009 5:11 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Eosin Bleeding > > We very occasionally have a single slide out of a group of 100 or more where the eosin will bleed after coverslipping. Does anyone know if this is associated with a particular tissue type or treatment with Nair to soften keratin? > > Thanks, > > Tresa > Veterinary Diagnostic Lab > Bozeman, MT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From macveigh <@t> usc.edu Wed Aug 5 17:55:22 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Wed Aug 5 17:55:22 2009 Subject: [Histonet] Re: working with mouse liver Message-ID: <002301ca161f$d03e6df0$5c237d80@DFS66DD1> Hi Teri, We have been discussing this option and left it at that. I have noticed that the livers, with more lipids look much closer to the textbook images. Michelle, Could it be what you are seeing is normal healthy (actively feeding) liver in mice and the missing cytoplasm is where the glycogen stores are/were? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From tkngflght <@t> yahoo.com Wed Aug 5 19:47:38 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Aug 5 19:47:42 2009 Subject: [Histonet] clearing agent with easy disposal? (adding to Bill's query) Message-ID: <190838.67115.qm@web50904.mail.re2.yahoo.com> Hi Everyone- ? >From the conversation started by Bill?it's clear?there are more than a few options for clearing agent chemicals (listed below without name brands). Could you all help put name brands with the type of clearant as that would help a lot? ? isopar derivative/ non-aliphatic hydrocarbon.? d-limonene ?? alkane derivatives mineral spirits Xylene/xylenes ? ? I was told by someone (not verified) that Clear-Rite 3 could be washed down the drain (don?t freak out, we haven?t done it) and I wanted to know if there really IS a a low odor clearing agent with easy disposal requirements for some of the smaller derm places we help that only stain (no processing) ? All responses are welcome. ? Thanks! ? Cheryl From ooi.ting.huay <@t> nhc.com.sg Thu Aug 6 01:24:20 2009 From: ooi.ting.huay <@t> nhc.com.sg (ooi.ting.huay@nhc.com.sg) Date: Thu Aug 6 01:24:32 2009 Subject: [Histonet] MMA and benzoyl peroxide Message-ID: Hi, I am doing MMA plastic embedding and have a problem with getting a cloudy embedded sample. I am using MMA plus perkadox 16. Not too sure whether the cloudiness is caused by perkadox 16. I would actually like to try to do the embedding using MMA and benzoyl peroxide. I would appreciate if anyone can share with me what kind of benzoyl peroxide and the working concentration that I should use. With the MMA and perkadox sample, I let the sample polymerize at room temperature. If I change to benzoyl peroxide, what kind of condition is the best for it to get polymerized? Thank you very much! Regards, Ooi _________________________________________________ Confidential information. Unauthorized use or disclosure prohibited. Refer http://www.singhealth.com.sg/ContactUs/#Disclaimer From doakes <@t> olympicmedical.org Thu Aug 6 07:05:27 2009 From: doakes <@t> olympicmedical.org (Dawn Oakes) Date: Thu Aug 6 07:22:28 2009 Subject: [Histonet] Tissuse Processors Message-ID: <01A05F75D299414EBFFB912EF6DAF66902B50948@is-210vs.olympicmedical.local> Does anyone have feedback on the difference between the Tissuse-Tek VIP 6 vs the Leica ASP200S. We are about to purchase a new processor. Dawn Oakes HT ASCP Olympic Medical Center ------------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. From sonya.martin <@t> soton.ac.uk Thu Aug 6 09:50:58 2009 From: sonya.martin <@t> soton.ac.uk (James S.) Date: Thu Aug 6 09:51:11 2009 Subject: [Histonet] ER labelling of fixed cells Message-ID: <5F338719C0D0DE44BDFDD2B83D3FF7A1B269AE5FBA@UOS-CL-EX7-L3.soton.ac.uk> Hi all, I'm trying to do some double labelling to show that a protein resides in the ER but I'm having some trouble getting nice reticulated ER staining in fixed cells. So far I have tried fixing in 4% PFA (15min on ice) followed by permeabilization with Tritonx100 (0.25%; 10min; room temp) or Saponin (0.5%; 7min; room temp; all susequent solutions containing 0.05% saponin). ER antibody is rabbit anti Calnexin (Stressgen). The labelling I'm getting is quite strong but is very punctate. Staining of my protein of interest is also very puntate but doesn't appear to colocalise with the clanexin and also I don't see any staining of the plasm membrane - which I should! Anyway the Prof wants to see nice reticulated staining of the ER - which I have seen in live cells with ER tracker but is it possible to get the same thing with fixed cells? I thought about trying to fix with glutaraldehyde but ?concentration ?time...... Also my PFA was in PBS would it be better to put it in HEPES or PIPES? Any suggestions welcome! Thanks Sonya From MLunetta <@t> luhcares.org Thu Aug 6 11:57:05 2009 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Thu Aug 6 11:57:29 2009 Subject: [Histonet] Histology job Opening, Colorado Message-ID: <4A7AB701020000A800034A4B@mail.luhcares.org> Hello Histo-Netters, We have an HT job opening here in Longmont Colorado, located north of Denver and just NE of Boulder. Please apply through the website. I have attached the link. All new and experianced HT's please apply. Also please add my name to your application so we can track the responces from histo net. http://luh.jobscience.com/JsrApp/index.cfm?cmd=showPositionDetail&nextEvent=doSearchPositions&cobrandId=9000&masterId=luh001&accountId=9F3D0B3C-E71A-17E7-69B649D49CA1C88F&positionId=511568&urlArgList=c2VhcmNoVHlwZT1xdWljayZzdGFydD0xJmNvdW50PTUwJmRlcHRJZD0mam9iSWQ9JmtleXdvcmQ9&bid=1297 If the link does not work the web site is www.luhcares.org Thanks Matt Lunetta BS, HT (ASCP) From vapatpxs <@t> yahoo.com Thu Aug 6 12:12:31 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Thu Aug 6 12:12:35 2009 Subject: [Histonet] Core unit support Message-ID: <554043.34139.qm@web46112.mail.sp1.yahoo.com> Hello Everybody, I was wondering what type of institutional support y'all have at your various institutions.? I work for a research foundation and they want to drop my salary and have the researchers grants pay my salary and almost everything else.? I am a fee-for-service lab and the CEO wants to see $25K in recharges between now and December. We offer TEM, confocal, histology and cryosectioning.? We will be adding micro-CT and a deconvolution microscope that does FRET and can handle samples that have been transfected(?) with viral vectors. Is she being unrealistic?? I am only 80% time and the only person down here.? Should I start looking for a new position before she shuts me down?? How many people have had their facilities closed by the people who don't see the big picture? Please let me know your experiences.? Thanks, Paula? :-} Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. From bakevictoria <@t> gmail.com Thu Aug 6 12:13:06 2009 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Aug 6 12:13:11 2009 Subject: [Histonet] metal embedding base molds Message-ID: <4f016b690908061013r6a410546j8a867e9813392a69@mail.gmail.com> Hi- I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm able to find at this point are the metal molds with the 'wings' on them. Does anyone know a source? With all the mergers etc it's been a little challenging. Thanks in advance Vikki From vapatpxs <@t> yahoo.com Thu Aug 6 12:14:19 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Thu Aug 6 12:14:22 2009 Subject: [Histonet] Support for core units Message-ID: <73199.21406.qm@web46104.mail.sp1.yahoo.com> Hello Everybody, I was wondering what type of institutional support y'all have at your various institutions. I work for a research foundation and they want to drop my salary and have the researchers grants pay my salary and almost everything else. I am a fee-for-service lab and the CEO wants to see $25K in recharges between now and December. We offer TEM, confocal, histology and cryosectioning. We will be adding micro-CT and a deconvolution microscope that does FRET and can handle samples that have been transfected(?) with viral vectors. Is she being unrealistic? I am only 80% time and the only person down here. Should I start looking for a new position before she shuts me down? How many people have had their facilities closed by the people who don't see the big picture? Please let me know your experiences. Thanks, Paula :-} Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. From portera <@t> msu.edu Thu Aug 6 12:30:34 2009 From: portera <@t> msu.edu (Amy Porter) Date: Thu Aug 6 12:30:44 2009 Subject: [Histonet] metal embedding base molds References: <4f016b690908061013r6a410546j8a867e9813392a69@mail.gmail.com> Message-ID: <08DEAF2AEF394D02817D86F01AD998AE@histolab> Try EM Sciences the distribute alot of the unique old type histology supplies. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Victoria Baker" To: "histonet" Sent: Thursday, August 06, 2009 1:13 PM Subject: [Histonet] metal embedding base molds > Hi- > > I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All > I'm > able to find at this point are the metal molds with the 'wings' on them. > > Does anyone know a source? With all the mergers etc it's been a little > challenging. > > Thanks in advance > > Vikki > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sbreeden <@t> nmda.nmsu.edu Thu Aug 6 13:08:44 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Aug 6 13:08:49 2009 Subject: [Histonet] Mycoplasma bovis Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46A05@nmdamailsvr.nmda.ad.nmsu.edu> My boss has thought up a new project for me so I need to know what I need to know about Mycoplasma bovis for IHC. He wants me to cost out the procedure. I normally do only TWO IHCs - CWD and BVD - so I'll be charting new mental territory with this one. Can anyone guide me on where to start figuring this out? Where do I get antibody? Is there a pre-existing protocol for the Ventana NexES? I don't believe in re-inventing the wheel if I don't need to. Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From b-frederick <@t> northwestern.edu Thu Aug 6 13:10:38 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Aug 6 13:10:51 2009 Subject: [Histonet] metal embedding base molds In-Reply-To: <08DEAF2AEF394D02817D86F01AD998AE@histolab> Message-ID: As well as surgipath. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Porter Sent: Thursday, August 06, 2009 12:31 PM To: Victoria Baker; histonet Subject: Re: [Histonet] metal embedding base molds Try EM Sciences the distribute alot of the unique old type histology supplies. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Victoria Baker" To: "histonet" Sent: Thursday, August 06, 2009 1:13 PM Subject: [Histonet] metal embedding base molds > Hi- > > I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All > I'm > able to find at this point are the metal molds with the 'wings' on them. > > Does anyone know a source? With all the mergers etc it's been a little > challenging. > > Thanks in advance > > Vikki > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jenee.S.Odani <@t> hawaii.gov Thu Aug 6 13:19:25 2009 From: Jenee.S.Odani <@t> hawaii.gov (Jenee.S.Odani@hawaii.gov) Date: Thu Aug 6 13:18:11 2009 Subject: [Histonet] Mycoplasma bovis In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46A05@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Hi there, Check out this database. It contains a contact list for the labs that are currently doing the Myco bovis IHC. http://ihc.sdstate.org/ -J Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 "Breeden, Sara" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/06/2009 08:12 AM To cc Subject [Histonet] Mycoplasma bovis My boss has thought up a new project for me so I need to know what I need to know about Mycoplasma bovis for IHC. He wants me to cost out the procedure. I normally do only TWO IHCs - CWD and BVD - so I'll be charting new mental territory with this one. Can anyone guide me on where to start figuring this out? Where do I get antibody? Is there a pre-existing protocol for the Ventana NexES? I don't believe in re-inventing the wheel if I don't need to. Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Katleba <@t> stjoe.org Thu Aug 6 13:23:21 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Thu Aug 6 13:23:30 2009 Subject: [Histonet] (no subject) Message-ID: <97C02552ECB11346877D3E83CF833ABD13D17C4880@SJSNT-SCMAIL03.stjoe.org> Hi All, Would anyone tell me what their lab procedure is for handling fetal demise placentas? In previous jobs sites, the histotech and/or PA would read the requisition and determine if there may be later DNA testing ordered. If yes, put in fridge and contact pathologist and/or physician. If not, place in formalin. Currently, our PA automatically places every placenta in formalin.... Kind regards, Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From NKlemme <@t> sakuraus.com Thu Aug 6 13:23:18 2009 From: NKlemme <@t> sakuraus.com (Nancy Klemme) Date: Thu Aug 6 13:24:38 2009 Subject: [Histonet] metal embedding base molds In-Reply-To: <4f016b690908061013r6a410546j8a867e9813392a69@mail.gmail.com> References: <4f016b690908061013r6a410546j8a867e9813392a69@mail.gmail.com> Message-ID: <782E3A02C2EB2347BEA6DEA69DC7AB865B4BFF12E7@sfamail.SAKURAUS.LOCAL> Dear Vikki, Sakura still carries the metal base molds that are used with Embedding Rings. They do not have the "wings" that the ones for use with the Unicassettes have. Their Sakura part numbers begin from 4121 and higher. If you use the electronic catalog on Sakura's website (www.sakuraus.com) and search by product description, just type in base molds. Then click on part # 4121 and the wingless ones will be pictured. Kind regards, Nancy Klemme, Sakura Educational Services -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Thursday, August 06, 2009 10:13 AM To: histonet Subject: [Histonet] metal embedding base molds Hi- I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm able to find at this point are the metal molds with the 'wings' on them. Does anyone know a source? With all the mergers etc it's been a little challenging. Thanks in advance Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jrobertson <@t> pathologysciences.com Thu Aug 6 14:24:53 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Thu Aug 6 14:24:57 2009 Subject: [Histonet] metal embedding base molds In-Reply-To: <4f016b690908061013r6a410546j8a867e9813392a69@mail.gmail.com> References: <4f016b690908061013r6a410546j8a867e9813392a69@mail.gmail.com> Message-ID: <518CD6920AA7154193CBE5977CD880733A8AFE@psmgsrv2.PSMG.local> Try Cardinal as they are a distributor for all Tissue Tek/Sakura supplies. Jodie Robertson, HT(ASCP)QIHC Histology Day Supervisor Pathology Sciences Medical Group -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Thursday, August 06, 2009 10:13 AM To: histonet Subject: [Histonet] metal embedding base molds Hi- I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm able to find at this point are the metal molds with the 'wings' on them. Does anyone know a source? With all the mergers etc it's been a little challenging. Thanks in advance Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MDiCarlo <@t> KaleidaHealth.Org Thu Aug 6 14:43:16 2009 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Thu Aug 6 14:43:22 2009 Subject: [Histonet] cement in bone specimen Message-ID: <1B73766A27A1554CB2729B6432E81301015C76BA@KALEXMB04.KaleidaHealth.org> Histonetters, We sliced a humerus bone on a band saw which was filled with cement from a previous surgery and my boss would like me to try to cut it on a sliding/sledge microtome. (The knife remains stationary) Is this possible to do? Presently, it is fixing and then I will decal it but I'm wondering if I'll be wasting my time and effort. I would appreciate anyone's experience with this. Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 2009 Best Places to Work Winner Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From Rick.Garnhart <@t> memorialhealthsystem.com Thu Aug 6 15:03:11 2009 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Thu Aug 6 15:03:16 2009 Subject: [Histonet] Re: Histonet Digest, Vol 69, Issue 6 In-Reply-To: Message-ID: Does anyone out in histology have any documentation from CMS, ASCP, or NSH that breaks down the job duties or areas for what is covered under Tech and professional part of the CPT billing codes. For example is Grossing part of the a profee or tech part of the bill. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. From macveigh <@t> usc.edu Thu Aug 6 15:03:33 2009 From: macveigh <@t> usc.edu (Michelle MacVeigh-Aloni) Date: Thu Aug 6 15:03:42 2009 Subject: [Histonet] What do you use for decolorizing after Hematox. Message-ID: <002401ca16d0$f9e4a3c0$5c237d80@DFS66DD1> Hi all, In my H&E staining I always used 1% acid alcohol. (Working with conc. HCl is not fun!) There is the Orth's solution available already made, but it is 3%. Do you use that? How do you use it? What do you use in your regulat H&E stain? I am using it on an old Jung AUTOSTAINER. Michelle Research Specialist USC Keck School of Medicine From ratliffjack <@t> hotmail.com Thu Aug 6 15:21:41 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Aug 6 15:21:47 2009 Subject: [Histonet] cement in bone specimen In-Reply-To: <1B73766A27A1554CB2729B6432E81301015C76BA@KALEXMB04.KaleidaHealth.org> References: <1B73766A27A1554CB2729B6432E81301015C76BA@KALEXMB04.KaleidaHealth.org> Message-ID: Peggy, If the cement you mention is or similar to PMMA, then you will not be able to cut thin sections using the sliding/sledge microtome. If I was you, I would not decal it at all as it will only make the bone less dense than the cement and will definitely waste your time if you embed it in paraffin to try and cut later. Your best and only chance at success here is to embed undemineralized in resin and cut thick or ground sections that can be polished to a desired thickness. If you would like to discuss this further, please give me a call at 317-281-1975. Best, Jack Ratliff > Date: Thu, 6 Aug 2009 15:43:16 -0400 > From: MDiCarlo@KaleidaHealth.Org > To: histonet@pathology.swmed.edu > CC: > Subject: [Histonet] cement in bone specimen > > Histonetters, > > > > We sliced a humerus bone on a band saw which was filled with cement from > a previous surgery and my boss would like me to try to cut it on a > sliding/sledge microtome. (The knife remains stationary) Is this > possible to do? Presently, it is fixing and then I will decal it but > I'm wondering if I'll be wasting my time and effort. > > > > I would appreciate anyone's experience with this. > > > > Thank you. > > > > Peggy DiCarlo HT (ASCP) > > Orthopedic Bone Lab > > Buffalo General Hospital > > Buffalo, NY 14203 > > 716-859-1293 > > > > > > 2009 Best Places to Work Winner > Visit our careers page at www.kaleidahealth.org/careers > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Thu Aug 6 15:53:16 2009 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Thu Aug 6 15:53:34 2009 Subject: [Histonet] Fetal demise placenta Message-ID: <8CBE4EF0E4C2479-93C-E73@webmail-me19.sysops.aol.com> Maria asks about fetal demise placenta: We gross in all fetal demise placenta (usually four blocks).If the clinicians desire genetics, they take?samples from the fetus and the placenta up on the floor. (There is only a three day window for growing fibroblasts). The fetus gets a gross only treatment with weight, measurements and a photograph. If the clinicians or family desire an autopsy, whatever the gestational age, we have the parents sign an autopsy consent, as if for an adult. Regards Michael Titford USA Pathology Mobile AL From Tiina.Vesterinen <@t> hus.fi Fri Aug 7 01:05:35 2009 From: Tiina.Vesterinen <@t> hus.fi (Vesterinen Tiina) Date: Fri Aug 7 01:06:27 2009 Subject: [Histonet] HPGD, PAFa and frizzled-4 stainings on Ventana Benchmark Message-ID: <2C0982D85C07A34598393F0B2B6F9675413886824E@vanhusmbx06.hus.fi> Hi everyone, Has anyone stained formalin fixed, paraffin embedded slices with HPGD (Sigma), PAF acetylhydrolase (Cayman Chemical) or frizzled-4 (Santa Cruz) on Ventana Discovery, Benchmark or similar autostainer? I would appreciate all advices related to staining protocols because I?ve quite small amounts of antibodies. Regards, Tiina Vesterinen Tiina Vesterinen Project Researcher Institute for Molecular Medicine Finland FIMM Helsinki, Finland From laurie.colbert <@t> huntingtonhospital.com Fri Aug 7 12:17:21 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Aug 7 12:17:27 2009 Subject: [Histonet] Specimen Release Message-ID: <57BE698966D5C54EAE8612E8941D7683064CEF2D@EXCHANGE3.huntingtonhospital.com> Does anyone have a procedure they would be willing to share with me regarding the release of specimens to the patient? We are revising our whole process and I'm interested in what others are doing. Laurie Colbert From juditw <@t> u.washington.edu Fri Aug 7 12:21:11 2009 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Fri Aug 7 12:21:24 2009 Subject: [Histonet] Good Fibrin stain with Movat's? Message-ID: Greetings to all histonetters- I have a pathologist that wants to show fibrin in lung. She wants also black nuclei and we tried a Movat's to look at fibrin. The fibrin in the lung is showing up light blue not red! Any suggestions on how to show fibrin distinctly, with black nuclei? thanks! Judy Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From thomas.crowell <@t> novartis.com Fri Aug 7 12:43:20 2009 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Fri Aug 7 12:43:35 2009 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 08/07/2009 and will not return until 08/10/2009. Please contact Humphrey Gardner at 617-871-3590 if you have any questions regarding clinical trial samples. From Rcartun <@t> harthosp.org Fri Aug 7 13:35:23 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Aug 7 13:35:35 2009 Subject: [Histonet] Specimen Release In-Reply-To: <57BE698966D5C54EAE8612E8941D7683064CEF2D@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D7683064CEF2D@EXCHANGE3.huntingtonhospital.com> Message-ID: <4A7C3BAB.7400.0077.1@harthosp.org> We no longer release specimens to patients. If they want their tissue they have to go through a funeral home. Here in CT, funeral homes are the only agencies licenced to bury or incinerate human tissue. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Laurie Colbert" 8/7/2009 1:17 PM >>> Does anyone have a procedure they would be willing to share with me regarding the release of specimens to the patient? We are revising our whole process and I'm interested in what others are doing. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kalschev <@t> svm.vetmed.wisc.edu Fri Aug 7 14:06:28 2009 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Fri Aug 7 14:06:27 2009 Subject: [Histonet] Peggy-cement in bone Message-ID: <002301ca1792$2b02fa80$c5d76880@vetmed.wisc.edu> Hi Peggy, From my experience, during the decal process the cement will fall out or loosen enough so that you can gently remove it and maintain fibrous ingrowth and detail. I have removed ceramic; implants; stents and cement using a scalpel and then embedded in paraffin for cuts on a sledge microtome. The detail is beautiful even without the cement present. If you have the capability, a corresponding section in PMMA can be processed. ~ Vicki From yashk <@t> gatech.edu Fri Aug 7 14:12:52 2009 From: yashk <@t> gatech.edu (Yash Kolambkar) Date: Fri Aug 7 14:12:58 2009 Subject: [Histonet] Stain for decalcified GMA embedded bone Message-ID: <4A7C7CB4.4080505@mail.gatech.edu> Hi, I am looking for a stain for formalin fixed, decalcified (2 weeks in CalEx II), GMA embedded rat femur samples that still has some muscle as well as fibrous tissue. I would like the stain to distinguish the bone from the muscle and fibrous tissue. I do not need to study the microstructure of the bone, just need something that can contrast the different tissues. I've tried the standard Masson's trichrome (with Aniline Blue), but the bone stained red and the contrast between the bone and the rest of the tissue is not strong. Same with H&E. Please let me know if you have any experience with such a stain. Any help would be much appreciated. Thanks! Yash -- Yash Kolambkar Graduate Research Assistant Department of Biomedical Engineering Georgia Tech/Emory Center for the Engineering of Living Tissues yashk@gatech.edu From amosbrooks <@t> gmail.com Sun Aug 9 06:03:07 2009 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Aug 9 06:03:11 2009 Subject: [Histonet] Good Fibrin stain with Movat's? Message-ID: <582736990908090403p616a1e85ta64b1cd546bdd0c0@mail.gmail.com> Hi, Have you tried a Sirius red (in sturated picric acid) with a Weigart's hamatoxylin for the Nucleii? I usually don't bother counterstaining the nucleii with this stain, but I suppose it would work. You wouuld probably want to do the hematoxylin after the Sirius red since it is an acidic solution and would bleach out the hematoxylin otherwise. While this isn't a Movat's I think it might do the trick for you. Amos Message: 2 Date: Fri, 7 Aug 2009 10:21:11 -0700 (PDT) From: "Judith L. Williams" Subject: [Histonet] Good Fibrin stain with Movat's? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed Greetings to all histonetters- I have a pathologist that wants to show fibrin in lung. She wants also black nuclei and we tried a Movat's to look at fibrin. The fibrin in the lung is showing up light blue not red! Any suggestions on how to show fibrin distinctly, with black nuclei? thanks! Judy From Jason.PALMER <@t> svhm.org.au Sun Aug 9 21:03:21 2009 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Sun Aug 9 21:03:29 2009 Subject: [Histonet] streptavidin-alexafluor conjugates Message-ID: Hi all. Just wondering what sort of experiences people have had with streptavidin-alexa fluor conjugates, and in particular AF350? (We want a blue dye conjugate to use in a double labelling procedure on tissue which has GFP labelled cells; CY-3 is used to detect the other Ab). I tried this reagent recently, at a range of dilutions, substituting it for a streptavidin-HRP label with an antibody and method that always works well on FFPE tissue, but got absolutely no signal. I do intend to try it again, but thought a histonet query might be a good idea at this point. I did come across a technical note (7.4, p 299 10th edition Mol Probes handbook) stating that "fluorophores conjugated to avidin and streptavidin may be quenched significantly, apparently because the dyes interact with amino acid residues in the biotin binding pocket...". It goes on to say that the addition of free biotin can help recover some of this lost signal. Does anyone have a comment or experience on such quenching, or tried free biotin blocking? Any comments or suggestions gratefully received, as always! Jason Jason Palmer Histology Laboratory Coordinator Bernard O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From deliadfam <@t> yahoo.com Mon Aug 10 00:03:13 2009 From: deliadfam <@t> yahoo.com (DELIA GARCIA) Date: Mon Aug 10 00:03:17 2009 Subject: [Histonet] Mouse Vs. Rabbit Message-ID: <88123.25980.qm@web63106.mail.re1.yahoo.com> Hi, I am? new to IHC so please excuse me if I dont ask this question properly. What is the difference between a mouse clone and rabbit clone? I came across a decision I had to make while purchasing an antibody. It was mentioned that usually a rabbit clone is the preferred. Is that true and why? Thank you so much! Delia From fong <@t> zoology.ubc.ca Mon Aug 10 01:39:06 2009 From: fong <@t> zoology.ubc.ca (Angelina Fong) Date: Mon Aug 10 01:39:09 2009 Subject: [Histonet] IHC on frozen vs paraffin embedded tissue Message-ID: <4A7FC08A.7080309@zoology.ubc.ca> Hi Everyone, I have a number of questions regarding paraffin embedding as I am a newbie to this type of tissue processing. I am wondering if anyone has any insight on doing IHC on frozen and paraffin embedded tissue for the same antigen. Is there much difference in the quality of the IHC, ie intensity of label (mostly fluorscence), distribution etc. Would you expect to be able to use similar concentration of antibody for both procedures? Would the IHC be comparable between the two tissue sets? The reason I am asking is that we have one tissue set (blood vessels) already processed for IHC using frozen cryocut sections, however, we have another set of tissue that may will be paraffin embedded for storage and transport from Sth America to Canada. Would we be able to use the same procedures that we have used so far on the frozen cryocut sections, on the paraffin embedded tissue? Or would we have to start all over again? Or does anyone know if you can 'retrieve' the tissue from the paraffin and then cryoprotect and freeze it? Thank you for any advice you might have! Cheers Angelina -- ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o Angelina Y. Fong, Ph.D. Department of Zoology Biological Sciences Building 6270 University Boulevard University of British Columbia Vancouver, BC, V6T 1Z4 Canada Ph: (604) 822-5799 Fax: (604) 822-2416 Email: fong@zoology.ubc.ca From joseph-galbraith <@t> uiowa.edu Mon Aug 10 08:36:15 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Mon Aug 10 08:36:25 2009 Subject: [Histonet] Mouse Vs. Rabbit In-Reply-To: <88123.25980.qm@web63106.mail.re1.yahoo.com> References: <88123.25980.qm@web63106.mail.re1.yahoo.com> Message-ID: Delia: Here is a link to a good overview article for the issues involved in your question. In the early days (and still somewhat true), the main difference between rabbit and mouse derived antibodies was polyclonal vs monoclonal. Today that distinction has been blurred somewhat as there are polyclonal mouse and monoclonal rabbit antibodies available for limited applications. Species selection is also driven by the need to derive an antibody in a species other than the target you intend to stain (though even that barrier has been bridged by systems like 'mouse on mouse' kits currently available). I hope this helps. http://dels.nas.edu/ilar_n/ilarjournal/46_3/pdfs/v4603Lipman.pdf Joe Galbraith University of Iowa joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DELIA GARCIA Sent: Monday, August 10, 2009 12:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse Vs. Rabbit Hi, I am? new to IHC so please excuse me if I dont ask this question properly. What is the difference between a mouse clone and rabbit clone? I came across a decision I had to make while purchasing an antibody. It was mentioned that usually a rabbit clone is the preferred. Is that true and why? Thank you so much! Delia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dencrowl <@t> MIT.EDU Mon Aug 10 08:42:52 2009 From: dencrowl <@t> MIT.EDU (Denise Crowley) Date: Mon Aug 10 08:42:58 2009 Subject: [Histonet] Human samples in university research lab Message-ID: Hi all, We are a research core facility processing animal tissues for cancer research. We have been approached by a collaborator about bringing human sample to us for processing, sectioning, and routine H&E staining, for use in research, not diagnosis. In the past, we have always encouraged these folks to have their slides cut at the clinical facility which is supplying the tissue. But we are getting more and more of these requests and I need to think about the changes we would need to make in our protocols, both in the processing schedules and safety issues. And I cannot even imagine the legal issues involved in transporting patient samples and informed consent. Can anyone give me some guidance here? Thanks, Denise Crowley Facility Manager Histology David H. Koch Institute for Integrative Cancer Research Massachusetts Institute of Technology 40 Ames St. E17-427 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu From Jessica.Vacca <@t> HCAhealthcare.com Mon Aug 10 09:05:44 2009 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Mon Aug 10 09:05:55 2009 Subject: [Histonet] CAP ? Message-ID: <938D716CD445614ABBB817517557B6F4C84766CE@NADCWPMSGCMS09.hca.corpad.net> How does one gather this information in the lab? What are your steps? GEN.70550 "Is there documentation that each of the chemicals in the laboratory has been evaluated for carcinogenic potential, reproductive toxicity, and acute toxicity; and does the policies and procedure manual define specific handling requirements for these chemicals" - Thanks Jessica Vacca Histology Supervisor 119 Oakfield Dr Brandon Fl 33511 (813) 571-5193 (813) 571-5169 FAX ? From TMcNemar <@t> lmhealth.org Mon Aug 10 09:32:46 2009 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Aug 10 09:32:57 2009 Subject: [Histonet] CAP ? In-Reply-To: <938D716CD445614ABBB817517557B6F4C84766CE@NADCWPMSGCMS09.hca.corpad.net> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E11E@lmhsmail.lmhealth.org> Most of that info should be available on the MSDS. Ours are available on the hospital network but we used to just keep a binder. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vacca Jessica Sent: Monday, August 10, 2009 10:06 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP ? How does one gather this information in the lab? What are your steps? GEN.70550 "Is there documentation that each of the chemicals in the laboratory has been evaluated for carcinogenic potential, reproductive toxicity, and acute toxicity; and does the policies and procedure manual define specific handling requirements for these chemicals" - Thanks Jessica Vacca Histology Supervisor 119 Oakfield Dr Brandon Fl 33511 (813) 571-5193 (813) 571-5169 FAX ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marirose.Satterfield <@t> MercyMemorial.org Mon Aug 10 09:43:28 2009 From: Marirose.Satterfield <@t> MercyMemorial.org (Satterfield, Marirose) Date: Mon Aug 10 09:43:49 2009 Subject: [Histonet] e-mail update Message-ID: How do I update my e-mail address? From joseph-galbraith <@t> uiowa.edu Mon Aug 10 10:24:31 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Mon Aug 10 10:24:47 2009 Subject: [Histonet] Good Fibrin stain with Movat's? In-Reply-To: <582736990908090403p616a1e85ta64b1cd546bdd0c0@mail.gmail.com> References: <582736990908090403p616a1e85ta64b1cd546bdd0c0@mail.gmail.com> Message-ID: Judy: Amos has a good suggestion. Below is a link to a Movat procedure from Penn, we discontinued our clinical Movat procedure due to lack of use (though it is a great stain). In the attached link, note the comments about the wash and differentiation steps. The Movat is nice in that it allows you to differentiate elastic, fibrin and collagen. Check after the alcian blue to see if the fibrin is already stained blue. Adjust concentrations and timing if necessary. Ensure that the alkaline alcohol is completely removed. Good luck. http://www.med.upenn.edu/mcrc/histology_core/movat.shtml Joe Galbraith University of Iowa joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Sunday, August 09, 2009 6:03 AM To: juditw@u.washington.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Fibrin stain with Movat's? Hi, Have you tried a Sirius red (in sturated picric acid) with a Weigart's hamatoxylin for the Nucleii? I usually don't bother counterstaining the nucleii with this stain, but I suppose it would work. You wouuld probably want to do the hematoxylin after the Sirius red since it is an acidic solution and would bleach out the hematoxylin otherwise. While this isn't a Movat's I think it might do the trick for you. Amos Message: 2 Date: Fri, 7 Aug 2009 10:21:11 -0700 (PDT) From: "Judith L. Williams" Subject: [Histonet] Good Fibrin stain with Movat's? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed Greetings to all histonetters- I have a pathologist that wants to show fibrin in lung. She wants also black nuclei and we tried a Movat's to look at fibrin. The fibrin in the lung is showing up light blue not red! Any suggestions on how to show fibrin distinctly, with black nuclei? thanks! Judy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccrowder <@t> vetmed.lsu.edu Mon Aug 10 10:27:21 2009 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Mon Aug 10 10:28:40 2009 Subject: [Histonet] Fixation of embryos Message-ID: Hello - A researcher is fixing 7-21 day old chicken embryos in 10% NBF. After processing they are mushy and almost amorphous. Has anyone any suggestions for a fixative that will give the embryos more form and still have the tissue capable of doing IHC? I had thought of 4F1G. Any help is really appreciated. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From diane.gladney <@t> us.army.mil Mon Aug 10 11:22:39 2009 From: diane.gladney <@t> us.army.mil (Gladney, Diane C Ms CIV USA MEDCOM MACH) Date: Mon Aug 10 11:22:59 2009 Subject: [Histonet] CAP ? (UNCLASSIFIED) In-Reply-To: <938D716CD445614ABBB817517557B6F4C84766CE@NADCWPMSGCMS09.hca.corpad.net> References: <938D716CD445614ABBB817517557B6F4C84766CE@NADCWPMSGCMS09.hca.corpad.net> Message-ID: <6673D9F600F29943A80ACD92795D345053E692@amedsermcbe042.amed.ds.army.mil> Classification: UNCLASSIFIED Caveats: NONE We have a MSDS for all of our chemicals. The information is listed in the MSDS. We keep copies in our MSDS Books in the lab for fast and easy access. The MSDS's are updated yearly, when we receive new chemicals or the manufacturer sends us their latest MSDS on their products. This use of the MSDS and the information listed is addressed in our Safety Standard Operating Procedures (SOP). We also list in this SOP all known carcinogens, reproductive toxicity and acute toxicity used in the lab. They are to be handled in accordance with the recommendations of the MSDS. Thanks, Diane G. Diane C. Gladney, HT (ASCP) Supervisor, Anatomical Pathology Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC? 29207 Email:? diane.gladney@amedd.army.mil Phone:? 803-751- 2530 FAX:???? 803-751-7829 DSN:??? 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vacca Jessica Sent: Monday, August 10, 2009 10:06 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP ? How does one gather this information in the lab? What are your steps? GEN.70550 "Is there documentation that each of the chemicals in the laboratory has been evaluated for carcinogenic potential, reproductive toxicity, and acute toxicity; and does the policies and procedure manual define specific handling requirements for these chemicals" - Thanks Jessica Vacca Histology Supervisor 119 Oakfield Dr Brandon Fl 33511 (813) 571-5193 (813) 571-5169 FAX ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE From mcauliff <@t> umdnj.edu Mon Aug 10 11:35:13 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Aug 10 11:33:33 2009 Subject: [Histonet] Fixation of embryos In-Reply-To: References: Message-ID: <4A804C41.2090400@umdnj.edu> If the G in 4F1G is glutaraldehyde the glut. will probably kill off the immunoreactivity (depending on the antigen in question). How long are the embryos being fixed? Remember, formalin works slowly so 5-7 days may be necessary.- I would try a formalin + alcohol mix or formalin-alcohol-acetic acid. Also, yolk is hard to fix well so don't use that as a criterion for good fixation. Geoff Cheryl Crowder wrote: > Hello - A researcher is fixing 7-21 day old chicken embryos in 10% NBF. > After processing they are mushy and almost amorphous. Has anyone any > suggestions for a fixative that will give the embryos more form and still > have the tissue capable of doing IHC? I had thought of 4F1G. Any help is > really appreciated. > Cheryl > > Cheryl Crowder, BA, HTL(ASCP) > Chief Technologist > Anatomic Pathology > Department of Pathobiological Sciences > School of Veterinary Medicine > Louisiana State University > Skip Bertman Drive > Baton Rouge, LA 70803 > > 225-578-9734 > FAX: 225-578-9720 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From bridget.maryott <@t> ventana.roche.com Mon Aug 10 11:50:54 2009 From: bridget.maryott <@t> ventana.roche.com (Maryott, Bridget) Date: Mon Aug 10 11:51:06 2009 Subject: [Histonet] Microm microtome maintenance in AZ Message-ID: <1975224336006344AA8326CAEF913DB007A820FA@rnumsem704.nala.roche.com> We are looking for someone to service our Microm microtomes in Tucson, AZ. Any ideas would be greatly appreciated. Bridget Maryott Advanced Staining Ventana Medical Systems, Inc. a member of the Roche Group 1910 E. Innovation Park Drive Tucson, Arizona 85755 Tel: +1 520 229 4022 mailto: bridget.maryott@ventana.roche.com Confidentiality Note: This message is intended only for the use of the named recipient(s) and may contain confidential and/or proprietary information. If you are not the intended recipient, please contact the sender and delete this message. Any unauthorized use of the information contained in this message is prohibited. From rjbuesa <@t> yahoo.com Mon Aug 10 11:54:45 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 10 11:54:50 2009 Subject: [Histonet] Fixation of embryos In-Reply-To: Message-ID: <499204.14057.qm@web65715.mail.ac4.yahoo.com> Use Bouin's fixative. Ren? J. --- On Mon, 8/10/09, Cheryl Crowder wrote: From: Cheryl Crowder Subject: [Histonet] Fixation of embryos To: "Histonet" Date: Monday, August 10, 2009, 11:27 AM Hello - A researcher is fixing 7-21 day old chicken embryos in 10% NBF.? After processing they are mushy and almost amorphous.? Has anyone any suggestions for a fixative that will give the embryos more form and still have the tissue capable of doing IHC????I had thought of 4F1G.? Any help is really appreciated. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jclark <@t> pcnm.com Mon Aug 10 12:39:53 2009 From: jclark <@t> pcnm.com (Joanne Clark) Date: Mon Aug 10 12:39:58 2009 Subject: [Histonet] RE: CAP Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C0109C296@mail.pcnm.com> ------------------------------ Message: 6 Date: Mon, 10 Aug 2009 09:05:44 -0500 From: Vacca Jessica Subject: [Histonet] CAP ? To: "Histonet@lists.utsouthwestern.edu" Message-ID: <938D716CD445614ABBB817517557B6F4C84766CE@NADCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="iso-8859-1" How does one gather this information in the lab? What are your steps? GEN.70550 "Is there documentation that each of the chemicals in the laboratory has been evaluated for carcinogenic potential, reproductive toxicity, and acute toxicity; and does the policies and procedure manual define specific handling requirements for these chemicals" - Thanks Jessica Vacca Histology Supervisor 119 Oakfield Dr Brandon Fl 33511 (813) 571-5193 (813) 571-5169 FAX ? We take this one step further than Mr. McNemar. We take the info from the MSDS and have prepared a chart of our toxic chemicals that is kept in the documentation of our Lab Safety. It lists for the chemicals involved the specific toxic dangers and any special handling. Joanne Clark, HT Histology Supervisor Pathology Consultants of NM Roswell, NM ------------------------------ From TJJ <@t> stowers.org Mon Aug 10 12:47:43 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Mon Aug 10 12:48:46 2009 Subject: [Histonet] Re: Human samples in university research lab Message-ID: Denise, You will need to contact your Institute Biosafety Committee (IBC) before you can start working on human samples in your lab. They should have everything you'll need to do in order to become compliant with federal regulations involving the use of human materials. It takes a ton of paperwork and preparation on the part of the collaborator and the university. In addition, anybody handling the material will need to take training so they are aware of the ethical and biohazardous issues involved. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 Message: 5 Date: Mon, 10 Aug 2009 09:42:52 -0400 From: Denise Crowley Subject: [Histonet] Human samples in university research lab To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Hi all, We are a research core facility processing animal tissues for cancer research. We have been approached by a collaborator about bringing human sample to us for processing, sectioning, and routine H&E staining, for use in research, not diagnosis. In the past, we have always encouraged these folks to have their slides cut at the clinical facility which is supplying the tissue. But we are getting more and more of these requests and I need to think about the changes we would need to make in our protocols, both in the processing schedules and safety issues. And I cannot even imagine the legal issues involved in transporting patient samples and informed consent. Can anyone give me some guidance here? Thanks, Denise Crowley Facility Manager Histology David H. Koch Institute for Integrative Cancer Research Massachusetts Institute of Technology 40 Ames St. E17-427 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu From zerfasp <@t> ors.od.nih.gov Mon Aug 10 13:50:50 2009 From: zerfasp <@t> ors.od.nih.gov (Zerfas, Patricia (NIH/OD/ORS) [E]) Date: Mon Aug 10 13:50:59 2009 Subject: [Histonet] CD31 working protocol Message-ID: Dear Listservers, I have tried MANY protocols and I am unable to obtain a working protocol for the CD31 antibody. I have tried both the CD31 rat monoclonal and a CD31 rabbit polyclonal. Thanks in advance for your help. Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 From portera <@t> msu.edu Mon Aug 10 14:14:05 2009 From: portera <@t> msu.edu (Amy Porter) Date: Mon Aug 10 14:14:16 2009 Subject: [Histonet] CD31 working protocol References: Message-ID: <98811FBE01C548AF99A3C982238F8D2B@histolab> BD Biosciences has a great rodent CD31 for mouse and rat - however you need to use their Zinc Fixative. It is well worth the results!! Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Zerfas, Patricia (NIH/OD/ORS) [E]" To: Sent: Monday, August 10, 2009 2:50 PM Subject: [Histonet] CD31 working protocol Dear Listservers, I have tried MANY protocols and I am unable to obtain a working protocol for the CD31 antibody. I have tried both the CD31 rat monoclonal and a CD31 rabbit polyclonal. Thanks in advance for your help. Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster <@t> umn.edu Mon Aug 10 14:38:07 2009 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Aug 10 14:38:09 2009 Subject: [Histonet] CD31 working protocol In-Reply-To: <98811FBE01C548AF99A3C982238F8D2B@histolab> References: <98811FBE01C548AF99A3C982238F8D2B@histolab> Message-ID: <4A80771F.3070508@umn.edu> Biocare has a great CD31 for mouse and rat too. You do need the kit and their primary but you can use formalin fixed samples with great results! Colleen Forster U of MN Amy Porter wrote: > BD Biosciences has a great rodent CD31 for mouse and rat - however you > need to use their Zinc Fixative. It is well worth the results!! > Amy S. Porter, HT (ASCP) QIHC > Investigative HistoPathology Laboratory - Supervisor > 2201 Biomedical Physical Sciences Bldg. Rm #2133 > East Lansing, MI 48824-3320 > Phone: (517) 884-5026 > Fax: (517) 432-1368 > Email: portera@msu.edu > Web: www.humanpathology.msu.edu > ----- Original Message ----- From: "Zerfas, Patricia (NIH/OD/ORS) [E]" > > To: > Sent: Monday, August 10, 2009 2:50 PM > Subject: [Histonet] CD31 working protocol > > > Dear Listservers, > I have tried MANY protocols and I am unable to obtain a > working protocol for the CD31 antibody. > I have tried both the CD31 rat monoclonal and a CD31 rabbit > polyclonal. > > Thanks in advance for your help. > > Patricia Zerfas > National Institutes of Health > Building 28A, Room 112 > 28 Library Drive > Bethesda, MD 20892 > ph: (301) 496-4464 > fax: (301) 402-1068 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Jackie.O'Connor <@t> abbott.com Mon Aug 10 14:44:02 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Aug 10 14:44:29 2009 Subject: [Histonet] CD31 working protocol In-Reply-To: Message-ID: Biocare has a CD31 anti mouse that does stain FFPE. I personally think nothing will ever compare with the Santa Cruz goat that no longer is available - but Biocare does work. "Zerfas, Patricia (NIH/OD/ORS) [E]" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/10/2009 01:50 PM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] CD31 working protocol Dear Listservers, I have tried MANY protocols and I am unable to obtain a working protocol for the CD31 antibody. I have tried both the CD31 rat monoclonal and a CD31 rabbit polyclonal. Thanks in advance for your help. Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From emerald_lake77 <@t> yahoo.com Mon Aug 10 15:00:01 2009 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Mon Aug 10 15:00:07 2009 Subject: [Histonet] CD31 working protocol In-Reply-To: Message-ID: <514929.47420.qm@web110606.mail.gq1.yahoo.com> Hi Patricia, ? What tissue (animal / human) are you using?? How has it been fixed /? processed? ? If you are using 10%NBF fixed, paraffin-embedded mouse tissue,?you?can use?Biocare's CD31 with a simple indirect protocol.? I come in with my primary (overnight at 5ug/ml) after Prot. K digestion (DAKO ready-to-use), and then come in with a secondary Alexa fluor 488 against my primary. ? It work really well.? I have also used it for frozen sections. ? I hope this helps. ? Regards. ? Gustave ? Gustave T. Hebert Research Scientist I Metabolic Disease Research Wyeth Research 200 CambridgePark Drive Cambridge, MA 02140 --- On Mon, 8/10/09, Zerfas, Patricia (NIH/OD/ORS) [E] wrote: From: Zerfas, Patricia (NIH/OD/ORS) [E] Subject: [Histonet] CD31 working protocol To: "'histonet@lists.utsouthwestern.edu'" Date: Monday, August 10, 2009, 2:50 PM Dear Listservers, ? ? ? ? ? ? I have tried MANY protocols and I am unable to obtain a working protocol for the CD31 antibody. ? ? ? ? ? ? I have tried both the CD31 rat monoclonal and a CD31 rabbit polyclonal. Thanks in advance for your help. Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD? 20892 ph:???(301) 496-4464 fax:? (301) 402-1068 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ebreisch <@t> rchsd.org Mon Aug 10 15:03:31 2009 From: ebreisch <@t> rchsd.org (Breisch, Eric) Date: Mon Aug 10 15:03:36 2009 Subject: [Histonet] cross contamination Message-ID: <43B97B4C402C2C44AAA2A8D2C86A88B303A52FB3@e2k3backend1.RCHSD.org> Hi Histonetters- Our laboratory does not process gynecologic cytology specimens and we have a relatively small number of non gynecologic specimens. Usually when we process the non gynecologic cytology specimens, they are done separately. I saw a CAP question which addresses prevention of cross contamination in the cytopathology section. Does anyone have a protocol which they could share which addresses the issue of "nongynecologic specimens that have a high potential for cross-contamination being stained separately from other nongynecologic specimens? Thanks in advance for your help. Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy Dept. of Surgery UCSD School of Medicine From garret.t.miyamoto <@t> us.army.mil Mon Aug 10 15:22:50 2009 From: garret.t.miyamoto <@t> us.army.mil (Miyamoto, Garret T Mr CIV USA USAMEDCOM) Date: Mon Aug 10 15:22:55 2009 Subject: [Histonet] Re: Specimen Release In-Reply-To: <0KO200ESAI2HP550@mail23.us.army.mil> References: <0KO200ESAI2HP550@mail23.us.army.mil> Message-ID: Laurie, I can fax you a copy of a release form if you want. Garret Miyamoto Tripler Army Medical Center ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Saturday, August 8, 2009 7:03 am Subject: Histonet Digest, Vol 69, Issue 8 To: histonet@lists.utsouthwestern.edu > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Specimen Release (Laurie Colbert) > 2. Good Fibrin stain with Movat's? (Judith L. Williams) > 3. Thomas Crowell is out of the office. > (thomas.crowell@novartis.com) 4. Re: Specimen Release (Richard > Cartun) 5. Peggy-cement in bone (Vicki Kalscheur) > 6. Stain for decalcified GMA embedded bone (Yash Kolambkar) > > > ------------------------------------------------------------------- > --- > > Message: 1 > Date: Fri, 7 Aug 2009 10:17:21 -0700 > From: "Laurie Colbert" < > Subject: [Histonet] Specimen Release > To: < > Message-ID: > < > > Content-Type: text/plain; charset="us-ascii" > > Does anyone have a procedure they would be willing to share with me > regarding the release of specimens to the patient? We are revising our > whole process and I'm interested in what others are doing. > > > > Laurie Colbert > > > > ------------------------------ > > Message: 2 > Date: Fri, 7 Aug 2009 10:21:11 -0700 (PDT) > From: "Judith L. Williams" < > Subject: [Histonet] Good Fibrin stain with Movat's? > To: histonet@lists.utsouthwestern.edu > Message-ID: > < > Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed > > > Greetings to all histonetters- I have a pathologist that wants to show fibrin in lung. She wants also black nuclei and we tried a Movat's to look at fibrin. The fibrin in the lung is showing up light blue not red! > Any suggestions on how to show fibrin distinctly, with black nuclei? > thanks! > Judy > > > > Judith Williams, PhD, HT(ASCP) > Research Scientist > Department of Comparative Medicine > University of Washington > Seattle, WA 98195 > > > > > > > ------------------------------ > > Message: 3 > Date: Fri, 7 Aug 2009 13:43:20 -0400 > From: thomas.crowell@novartis.com > Subject: [Histonet] Thomas Crowell is out of the office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > < > > Content-Type: text/plain; charset=US-ASCII > > > I will be out of the office starting 08/07/2009 and will not return until > 08/10/2009. > > Please contact Humphrey Gardner at 617-871-3590 if you have any questions > regarding clinical trial samples. > > ------------------------------ > > Message: 4 > Date: Fri, 07 Aug 2009 14:35:23 -0400 > From: "Richard Cartun" < > Subject: Re: [Histonet] Specimen Release > To: "Laurie Colbert" <, > < > Message-ID: < > Content-Type: text/plain; charset=US-ASCII > > We no longer release specimens to patients. If they want their tissue they have to go through a funeral home. Here in CT, funeral homes are the only agencies licenced to bury or incinerate human tissue. > > Richard > > Richard W. Cartun, Ph.D. > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > >>> "Laurie Colbert" < 8/7/2009 1:17 PM >>> > Does anyone have a procedure they would be willing to share with me > regarding the release of specimens to the patient? We are revising our > whole process and I'm interested in what others are doing. > > > > Laurie Colbert > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 5 > Date: Fri, 7 Aug 2009 14:06:28 -0500 > From: "Vicki Kalscheur" < > Subject: [Histonet] Peggy-cement in bone > To: "Histonet Discussion" < > Message-ID: < > Content-Type: text/plain; charset="iso-8859-1" > > Hi Peggy, From my experience, during the decal process the cement will fall out or loosen enough so that you can gently remove it and maintain fibrous ingrowth and detail. I have removed ceramic; implants; stents and cement using a scalpel and then embedded in paraffin for cuts on a sledge microtome. The detail is beautiful even without the cement present. If you have the capability, a corresponding section in PMMA can be processed. ~ Vicki > > ------------------------------ > > Message: 6 > Date: Fri, 07 Aug 2009 15:12:52 -0400 > From: Yash Kolambkar < > Subject: [Histonet] Stain for decalcified GMA embedded bone > To: histonet@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi, > > I am looking for a stain for formalin fixed, decalcified (2 weeks in > CalEx II), GMA embedded rat femur samples that still has some muscle as > well as fibrous tissue. I would like the stain to distinguish the bone > from the muscle and fibrous tissue. I do not need to study the > microstructure of the bone, just need something that can contrast the > different tissues. > > I've tried the standard Masson's trichrome (with Aniline Blue), but the > bone stained red and the contrast between the bone and the rest of the > tissue is not strong. Same with H&E. > > Please let me know if you have any experience with such a stain. Any > help would be much appreciated. > > Thanks! > > Yash > -- > Yash Kolambkar > Graduate Research Assistant > Department of Biomedical Engineering > Georgia Tech/Emory Center for the Engineering of Living Tissues > yashk@gatech.edu > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 69, Issue 8 > *************************************** From ssesodia <@t> mail.barry.edu Mon Aug 10 15:32:34 2009 From: ssesodia <@t> mail.barry.edu (Sesodia, Sanjay) Date: Mon Aug 10 15:32:40 2009 Subject: [Histonet] Cryostat repair/maintenance Message-ID: Dies anyone know of a reputable company that repairs/maintains cryostat microtomes in South Florida (Miami area)? From dunatrsd <@t> sbcglobal.net Mon Aug 10 17:41:21 2009 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Mon Aug 10 17:41:26 2009 Subject: [Histonet] CD31 working protocol In-Reply-To: References: <499535.10154.qm@web83910.mail.sp1.yahoo.com> Message-ID: <19384.96133.qm@web83910.mail.sp1.yahoo.com> Hi Patricia, I use an antibody from Spring Biosciences Cat#M3384 to stain our human xenograft tumors as well as human tissue. Antibody?will also stain?mouse tissue. Very nice clean crisp stain. At least when we use it on our Bond IHC stainiers. Thanks and let me know if you need more information. dusko ________________________________ From: "Zerfas, Patricia (NIH/OD/ORS) [E]" To: dusko trajkovic Sent: Monday, August 10, 2009 12:07:29 PM Subject: RE: [Histonet] CD31 working protocol Dusko, ??????????? I am using mouse tissue. ? Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD ? 20892 ph:?? (301) 496-4464 fax:? (301) 402-1068 ? ________________________________ From:dusko trajkovic [mailto:dunatrsd@sbcglobal.net] Sent: Monday, August 10, 2009 3:06 PM To: Zerfas, Patricia (NIH/OD/ORS) [E] Subject: Re: [Histonet] CD31 working protocol ? What species are you trying to stain? thanks Dusko ? ________________________________ From:"Zerfas, Patricia (NIH/OD/ORS) [E]" To: " histonet@lists.utsouthwestern.edu " < histonet@lists.utsouthwestern.edu > Sent: Monday, August 10, 2009 11:50:50 AM Subject: [Histonet] CD31 working protocol Dear Listservers, ? ? ? ? ? ? I have tried MANY protocols and I am unable to obtain a working protocol for the CD31 antibody. ? ? ? ? ? ? I have tried both the CD31 rat monoclonal and a CD31 rabbit polyclonal. Thanks in advance for your help. Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda , MD ? 20892 ph:? (301) 496-4464 fax:? (301) 402-1068 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jseaton <@t> wlgore.com Mon Aug 10 18:03:12 2009 From: jseaton <@t> wlgore.com (Janella Seaton) Date: Mon Aug 10 18:03:22 2009 Subject: [Histonet] Janella Seaton/WLGORE is out of the office. Message-ID: I will be out of the office starting 08/10/2009 and will not return until 08/13/2009. I will respond to your message when I return. Any histology-related requests or questions can be sent to: histology@wlgore.com. From Marilyn.A.Weiss <@t> kp.org Mon Aug 10 18:01:54 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Mon Aug 10 18:03:23 2009 Subject: [Histonet] marilyn weiss will be out of the office Message-ID: I will be out of the office starting 08/10/2009 and will not return until 08/11/2009. I will respond to your message when I return.In my absence please ask for Mary Campbell or Laurie at 619-528-6801 if this is urgent they can contact me or give you my cell phone number. From lab.mef <@t> smmc.org Tue Aug 11 06:49:44 2009 From: lab.mef <@t> smmc.org (Meredith Fuller-Fedorczyk) Date: Tue Aug 11 06:49:55 2009 Subject: [Histonet] (no subject) Message-ID: <01c901ca1a79$d1e71db0$240c0180@PCLAB11> What are the special stain chemicals that need to be shipped out as Hazardous Waste and not go down the drain into the sewer system. We know silver,chromic,gold,formalin and alcohols. What about 30 ml of Carbol Fuschsin solution or working iron stain? Any clarification on this will be helpful. Meredith Fuller-Fedorczyk Senior Histologist Southern Maine Medical Center 207.283.7184 From sbreckenridge <@t> caperegional.com Tue Aug 11 06:56:02 2009 From: sbreckenridge <@t> caperegional.com (Breckenridge, Sue) Date: Tue Aug 11 06:56:08 2009 Subject: [Histonet] Meditech and cassette labeler's Message-ID: <4D95C24EC0E4C84787A6919F1165B25E023EB036@btmhems01.BTHOSP.INT> Does anyone currently using Meditech version 5.62 for slide and/or cassette labeling (via vendor slide/cassette labeling devices)? If so could you please contact me? I am interested in other's experiences. Thank-you, Sue Breckenridge, Histology Supervisor Cape Regional Medical Center Cape May Court House, NJ Confidentiality Notice: This e-mail message, including any attachments, from Cape Regional Health System contains information which is CONFIDENTIAL AND/OR LEGALLY PRIVILEGED. The information is intended only for the use of the individual named above and may not be disseminated to any other party without Cape Regional Health System's written permission. If you are not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this e-mailed information is strictly prohibited. If you have received this e-mail in error, please notify us immediately by telephone at 609-463-2163 to arrange for the return of these documents to us without cost to you. From srishan <@t> mail.holyname.org Tue Aug 11 10:12:51 2009 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Tue Aug 11 10:13:40 2009 Subject: [Histonet] XXXL Latex Gloves Message-ID: Hi, Does anyone know a company who makes XXXL latex gloves? We do have a morgue attendant who claims the XXL gloves that we purchased(after a very long search) is too tight. Any help will be greatly appreciated. Nirmala Srishan Holy Name Hospital Teaneck, NJ __________________ What are the special stain chemicals that need to be shipped out as Hazardous Waste and not go down the drain into the sewer system. We know silver,chromic,gold,formalin and alcohols. What about 30 ml of Carbol Fuschsin solution or working iron stain? Any clarification on this will be helpful. Meredith Fuller-Fedorczyk Senior Histologist Southern Maine Medical Center 207.283.7184 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Hospital is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Sixth Nationally by Modern Healthcare , 2008 Best Places to Work in New Jersey, NJBIZ - 2006, 2007, 2008 & 2009 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power - 2006 & 2007 Distinguished Hospital Awards for Clinical Excellence, HealthGrades - 2005, 2006, 2007 & 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades - 2008 & 2009 Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From denise.woodward <@t> uconn.edu Tue Aug 11 10:22:22 2009 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Tue Aug 11 10:22:31 2009 Subject: [Histonet] whipf's polychrome procedure Message-ID: Hello all, One of our veterinary pathology residents from France is looking for a staining procedure for "Whipf's Polychrome". Anyone have it and willing to share? Thanks, Denise Long Woodward University of Connecticut Connecticut Veterinary Medical Diagnostic Laboratory Dept. of Pathobiology and Veterinary Sciences From burch007 <@t> mc.duke.edu Tue Aug 11 10:30:30 2009 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Tue Aug 11 10:30:38 2009 Subject: [Histonet] whipf's polychrome procedure In-Reply-To: Message-ID: Here is the polychrome formula I use. Jim Polychrome Methylene Blue Stain Tissue and Fixation Frozen sections and formalin-fixed, paraffin embedded Objective Rapid differential staining of tissue sections. Metachromatic stain. Reagents Stock solutions: A. 12% aqueous potassium carbonate, 100 ml. B. 1% aqueous methylene blue, 4000 ml. C. 10% aqueous glacial acetic acid, 50 ml. Staining solutions: 1. Mix 100 ml of A with 5000 ml of B. 2. Gently boil for 60 minutes. Cool to room temperature. 3. Add 50 ml of C. 4. Store in a loosely capped bottle for further oxidation. Procedure 1. Dip frozen sections in stain for 5 seconds. 2. Rinse excess stain from slide. 3. Mount in water with a cover glass if desired. Results Nuclei blue, cytoplasm, muscle, connective tissue, pink to purple. Note: The sections are not permanent. Solution works best after aging for at least 12 months, recognizable by a rich tinctoral quality. Increase stock solution volumes to make a larger quantity of working stain. Filter before use. Reference: Terry, BT, J. Lab and Clinical Medicine, Vol. 13, page 550, 1928. "Woodward, Denise" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/11/2009 11:23 AM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] whipf's polychrome procedure Hello all, One of our veterinary pathology residents from France is looking for a staining procedure for "Whipf's Polychrome". Anyone have it and willing to share? Thanks, Denise Long Woodward University of Connecticut Connecticut Veterinary Medical Diagnostic Laboratory Dept. of Pathobiology and Veterinary Sciences _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLunetta <@t> luhcares.org Tue Aug 11 10:31:51 2009 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Tue Aug 11 10:32:14 2009 Subject: [Histonet] JOB OPENING IN COLORADO Message-ID: <4A813A88020000A800034E41@mail.luhcares.org> Hello Histo-Netters, We have an HT job opening here in Longmont Colorado, located north of Denver and just NE of Boulder. Please apply through the website. I have attached the link. All new and experianced HT's please apply. Also please add my name to your application so we can track the responces from histo net. http://luh.jobscience.com/JsrApp/index.cfm?cmd=showPositionDetail&nextEvent=doSearchPositions&cobrandId=9000&masterId=luh001&accountId=9F3D0B3C-E71A-17E7-69B649D49CA1C88F&positionId=511568&urlArgList=c2VhcmNoVHlwZT1xdWljayZzdGFydD0xJmNvdW50PTUwJmRlcHRJZD0mam9iSWQ9JmtleXdvcmQ9&bid=1297 If the link does not work the web site is www.luhcares.org Thanks Matt Lunetta BS, HT (ASCP) From NSEARCY <@t> swmail.sw.org Tue Aug 11 11:20:27 2009 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Tue Aug 11 11:20:41 2009 Subject: [Histonet] Finance Question Message-ID: <4A8153FB.5D38.00EF.0@swmail.sw.org> Any institutions / laboratories where bone marrows are performed have an issue with Bone Marrows and what department gets what revenue / workload, etc. There are no CPT codes for the "technical work" that is performed by the hematology technicians only the Surgical Pathology charges. (save for 85097- Bone marrow smear interpretation , a professional charge). Technically speaking, about 1/3 of the technical work is done in hematology and 2/3 in histology but we have a discussion as who is to get the revenue because only one charge master code can be attached to the test. Frankly, the revenue stays in the laboratory, but when work stats are performed and cost / test is being evaluated, the administrators want a true cost per test. If there are hundreds of bone marrows, then this revenue can become an issue between hematology and surgical pathology. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division TEL;PAGER:633-2370 END:VCARD From rjbuesa <@t> yahoo.com Tue Aug 11 12:04:47 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 11 12:04:51 2009 Subject: [Histonet] Finance Question In-Reply-To: <4A8153FB.5D38.00EF.0@swmail.sw.org> Message-ID: <753423.66959.qm@web65704.mail.ac4.yahoo.com> All bone marrows (aspirates and biopsies) were handled and credited to the?histology cost center?at my institution. Ren? J. --- On Tue, 8/11/09, Nita Searcy wrote: From: Nita Searcy Subject: [Histonet] Finance Question To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 11, 2009, 12:20 PM Any institutions / laboratories where bone marrows are performed have an issue with Bone Marrows and what department gets what revenue / workload, etc. There are no CPT codes for the "technical work" that is performed by the hematology technicians only the Surgical Pathology charges.? (save for 85097- Bone marrow smear interpretation , a professional charge). Technically speaking, about 1/3 of the technical work is done in hematology and 2/3 in histology but we have a discussion as who is to get the revenue because only one charge master code can be attached to the test. Frankly, the revenue? stays in the laboratory, but when work stats are performed and cost / test is being evaluated, the administrators want a true cost per test. If there are hundreds of bone marrows, then this revenue can become an issue between hematology and surgical pathology. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Colleen.Spees <@t> osumc.edu Tue Aug 11 12:25:07 2009 From: Colleen.Spees <@t> osumc.edu (Spees, Colleen) Date: Tue Aug 11 12:27:04 2009 Subject: [Histonet] IHC - p53 analysis Message-ID: <6B5A8F5234DA9F46891A492A84BFA7FA1C3350@msxc07.OSUMC.EDU> I am a Graduate Fellow working with access to Image Pro Plus (4.5.1) for analyzing human prostate tissues - staining for p53. The lab folks that were experienced in this area have since moved on, so I'm starting from scratch and had a few questions: 1. I see that Image Pro Plus has released version 7.0. I'm wondering if this expensive upgrade is worth it for p53 IHC analysis or if anyone has any experience with IPP. 2. I understand that analysis for p53 IHC is quite challenging. I wondered if anyone had any general insights they could share - other (but not too expensive) software that would be better to use; preference for hand counting vs. computer counting; etc. 3. Other references or resources that could further enlighten me in this area or other points I need to consider. Colleen Spees Medical Dietetics School of Allied Medical Professions The Ohio State University 306 Atwell Hall 453 West Tenth Avenue Columbus, Ohio 43210-1234 colleen.spees@osumc.edu 614-688-4651 614-266-9234 (c) From BoozerKA <@t> ah.org Tue Aug 11 13:02:48 2009 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Tue Aug 11 13:02:59 2009 Subject: [Histonet] Finance Question In-Reply-To: <753423.66959.qm@web65704.mail.ac4.yahoo.com> References: <4A8153FB.5D38.00EF.0@swmail.sw.org> <753423.66959.qm@web65704.mail.ac4.yahoo.com> Message-ID: <4A814FD3.4AA8.00C0.0@ah.org> We are asking the same questions and in addition, FNA help. We are looking at 99000 and 99001 which has something to do with assisting the physician. I don't have the correct wording but I have an expert coming tomorrow who can clarify. Kathy Boozer Kathy Boozer, HT (ASCP), IHCQ Adventist Medical Center Portland, OR boozerka@pa.org >>> Rene J Buesa 08/11/2009 10:04 >>> All bone marrows (aspirates and biopsies) were handled and credited to the histology cost center at my institution. Ren? J. --- On Tue, 8/11/09, Nita Searcy wrote: From: Nita Searcy Subject: [Histonet] Finance Question To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 11, 2009, 12:20 PM Any institutions / laboratories where bone marrows are performed have an issue with Bone Marrows and what department gets what revenue / workload, etc. There are no CPT codes for the "technical work" that is performed by the hematology technicians only the Surgical Pathology charges. (save for 85097- Bone marrow smear interpretation , a professional charge). Technically speaking, about 1/3 of the technical work is done in hematology and 2/3 in histology but we have a discussion as who is to get the revenue because only one charge master code can be attached to the test. Frankly, the revenue stays in the laboratory, but when work stats are performed and cost / test is being evaluated, the administrators want a true cost per test. If there are hundreds of bone marrows, then this revenue can become an issue between hematology and surgical pathology. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Tue Aug 11 13:36:18 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Aug 11 13:36:23 2009 Subject: [Histonet] ISH Message-ID: <65365F35C0F2EF4D846EC3CA73E49C438E0A2E1FAA@HPEMX3.HealthPartners.int> Can anyone supply me with a vendor for Kappa/Lambda ISH? I heard that Dako and Ventana have had to take their ASR Kappa.Lamda product off of the market. I am in need of where to go for ASR or IVD in this area!! Thanks, Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From mward <@t> wfubmc.edu Tue Aug 11 13:42:27 2009 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Aug 11 13:44:56 2009 Subject: [Histonet] LYVE-1 antibody Message-ID: <61135F0455D33347B5AAE209B903A30429D3500A@EXCHVS2.medctr.ad.wfubmc.edu> Hello, I wanted to know if anyone has tried this antibody on the Bond stainer, and if so, how did it work for you? I have purchased one from Santa Cruz Biotechnology (cat#sc-80170) at the request of one of our Pathologists. Any feedback is appreciated. Thanks! Martha Ward Molecular Diagnostics Lab Wake Forest University Baptist Medical Center From jrobertson <@t> pathologysciences.com Tue Aug 11 13:44:56 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Tue Aug 11 13:45:01 2009 Subject: [Histonet] ISH In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C438E0A2E1FAA@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C438E0A2E1FAA@HPEMX3.HealthPartners.int> Message-ID: <518CD6920AA7154193CBE5977CD880733A8B0F@psmgsrv2.PSMG.local> Ventana has put their Kappa/Lambda back on the market in ASR format. Jodie Robertson, HT(ASCP)QIHC Histology Day Supervisor Pathology Sciences Medical Group -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, August 11, 2009 11:36 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ISH Can anyone supply me with a vendor for Kappa/Lambda ISH? I heard that Dako and Ventana have had to take their ASR Kappa.Lamda product off of the market. I am in need of where to go for ASR or IVD in this area!! Thanks, Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Aug 11 14:59:08 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Aug 11 14:58:54 2009 Subject: [Histonet] ISH In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C438E0A2E1FAA@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C438E0A2E1FAA@HPEMX3.HealthPartners.int> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA57400C9@ITSSSXM01V6.one.ads.che.org> We use Leica - works perfectly!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, August 11, 2009 14:36 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ISH Can anyone supply me with a vendor for Kappa/Lambda ISH? I heard that Dako and Ventana have had to take their ASR Kappa.Lamda product off of the market. I am in need of where to go for ASR or IVD in this area!! Thanks, Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From wlecorch <@t> rwjuhh.edu Tue Aug 11 15:35:27 2009 From: wlecorch <@t> rwjuhh.edu (Lecorchick, William) Date: Tue Aug 11 15:35:41 2009 Subject: [Histonet] cross contamination In-Reply-To: <1250008757.971072@messagescreen2.rwjham.net> Message-ID: <09411E0112A96A459D8D5FBDAB9C15C71A4AB789BA@HAMEXMBA.rwjham.local> When you run your stain place a blank slide in the rack and evaluate the slide for any "floaters" label and file as you would a control slide. From alyssa <@t> alliedsearchpartners.com Tue Aug 11 16:30:05 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Aug 11 16:30:08 2009 Subject: [Histonet] Job Opening Pathologist Assistant-FL Message-ID: Allied Search Partners is now accepting resumes for client of Tallahassee, FL. We are accepting the following resumes for permanent/direct hire position: Pathologist Assistant Shifts: Day Full Time $Cash Bonuses For Referrals$ Please submit your resume for prescreening purposes to alyssa@alliedsearchpartners.com *All inquiries are always kept confidential* Be sure to visit our website www.alliedsearchpartners.com to submit your job search request, refer a friend for $$Cash Bonus$$, and have your resume reviewed by our career advisors. -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From Jason.PALMER <@t> svhm.org.au Tue Aug 11 19:23:07 2009 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Tue Aug 11 19:23:14 2009 Subject: [Histonet] re: BD CD31 Message-ID: Actually we have been using BD CD31 on mouse tissue with routine FFPE fixation with good results for a while now - Zn fixation not needed. Dako proteinase K 8 min, primary at 1:150, biotinylated secondary then Vector ABC with DAB substrate works well for us. Cheers, Jason Jason Palmer Histology Laboratory Coordinator Bernard O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au ------------------------------ Message: 4 Date: Mon, 10 Aug 2009 15:14:05 -0400 From: "Amy Porter" Subject: Re: [Histonet] CD31 working protocol To: "Zerfas, Patricia \(NIH/OD/ORS\) [E]" , Message-ID: <98811FBE01C548AF99A3C982238F8D2B@histolab> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original BD Biosciences has a great rodent CD31 for mouse and rat - however you need to use their Zinc Fixative. It is well worth the results!! Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Zerfas, Patricia (NIH/OD/ORS) [E]" To: Sent: Monday, August 10, 2009 2:50 PM Subject: [Histonet] CD31 working protocol Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From ccrowder <@t> vetmed.lsu.edu Tue Aug 11 20:51:54 2009 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Tue Aug 11 20:52:53 2009 Subject: [Histonet] Embryo fixation Message-ID: Thank all of you who answered by question about fixation of chicken embryos. We have decided to try alcoholic formalin on the early embryos and add acetic acid to the older ones with calcification of the calvarium. Wish us luck. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From jencres <@t> ca.rr.com Tue Aug 11 21:22:16 2009 From: jencres <@t> ca.rr.com (jennifer cresor mike hough) Date: Tue Aug 11 21:22:18 2009 Subject: [Histonet] Slide Brite xylene substitute Message-ID: Hello all, Does anyone out there have experiences with Slide Brite xylene substitute? Did you have any problems? Lately, I have been finding what appears to be water on my slides. Under the microscope it looks like pink gobules on the slide and the tissue. The last 2 days I changed out all of my stains and reagents to see if that would cure the problem and it did not. It is very random, not on all of the slides. But, when these gobules are on the slide it changes the stain dramatically to a dark blue appearing hematoxylin. Any thoughts? I am considering whether to change to a different xylene substitute. Any recommendations. Thank you for your response. Jennifer Temecula, California jencres@ca.rr.com From dmccaig <@t> ckha.on.ca Tue Aug 11 21:28:21 2009 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Tue Aug 11 21:28:27 2009 Subject: [Histonet] base molds Message-ID: I have a few base molds for needle core biopsies that will allow 8-10 levels/ slide. The molds are about 25 x 6 cm. Does anyone know of a supplier? Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential and or proprietary information and is provided for the use of the intended recipient only. Any review, retransmission or dissemination of this information by anyone other than the intended recipient is prohibited. If you receive this email in error, please contact the sender and delete this communication and any copies immediately. Thank you. From histopathy <@t> hotmail.com Tue Aug 11 21:56:43 2009 From: histopathy <@t> hotmail.com (Pamela Gholston) Date: Tue Aug 11 21:56:51 2009 Subject: [Histonet] RE: Histonet Digest, Vol 69, Issue 9 In-Reply-To: References: Message-ID: Hello Histotechs!! Has anyone had any problems with the "cornflaking artifact?" It looks as though the tissue has brown artifact on it. Some of the slides look like there is water in the tissue. It seems like a potential xylene purity problem. Can I get some opinions? Histopathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, August 09, 2009 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 69, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Good Fibrin stain with Movat's? (Amos Brooks) ---------------------------------------------------------------------- Message: 1 Date: Sun, 9 Aug 2009 07:03:07 -0400 From: Amos Brooks Subject: [Histonet] Good Fibrin stain with Movat's? To: juditw@u.washington.edu, histonet@lists.utsouthwestern.edu Message-ID: <582736990908090403p616a1e85ta64b1cd546bdd0c0@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi, Have you tried a Sirius red (in sturated picric acid) with a Weigart's hamatoxylin for the Nucleii? I usually don't bother counterstaining the nucleii with this stain, but I suppose it would work. You wouuld probably want to do the hematoxylin after the Sirius red since it is an acidic solution and would bleach out the hematoxylin otherwise. While this isn't a Movat's I think it might do the trick for you. Amos Message: 2 Date: Fri, 7 Aug 2009 10:21:11 -0700 (PDT) From: "Judith L. Williams" Subject: [Histonet] Good Fibrin stain with Movat's? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed Greetings to all histonetters- I have a pathologist that wants to show fibrin in lung. She wants also black nuclei and we tried a Movat's to look at fibrin. The fibrin in the lung is showing up light blue not red! Any suggestions on how to show fibrin distinctly, with black nuclei? thanks! Judy ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 69, Issue 9 *************************************** From histopathy <@t> hotmail.com Tue Aug 11 21:58:37 2009 From: histopathy <@t> hotmail.com (Pamela Gholston) Date: Tue Aug 11 21:58:46 2009 Subject: [Histonet] RE: Histonet Digest, Vol 69, Issue 9 In-Reply-To: References: Message-ID: Hello Histotechs! What is everyone's opinion of the quality of the 5.2 Gallon drum of xylene (Fisher) verses the 1 gallon (Cardinal or Fisher). Has anyone had a purity problem with the xylene in the 5.2 gallons verses the 1 gallon? Histopathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, August 09, 2009 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 69, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Good Fibrin stain with Movat's? (Amos Brooks) ---------------------------------------------------------------------- Message: 1 Date: Sun, 9 Aug 2009 07:03:07 -0400 From: Amos Brooks Subject: [Histonet] Good Fibrin stain with Movat's? To: juditw@u.washington.edu, histonet@lists.utsouthwestern.edu Message-ID: <582736990908090403p616a1e85ta64b1cd546bdd0c0@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi, Have you tried a Sirius red (in sturated picric acid) with a Weigart's hamatoxylin for the Nucleii? I usually don't bother counterstaining the nucleii with this stain, but I suppose it would work. You wouuld probably want to do the hematoxylin after the Sirius red since it is an acidic solution and would bleach out the hematoxylin otherwise. While this isn't a Movat's I think it might do the trick for you. Amos Message: 2 Date: Fri, 7 Aug 2009 10:21:11 -0700 (PDT) From: "Judith L. Williams" Subject: [Histonet] Good Fibrin stain with Movat's? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed Greetings to all histonetters- I have a pathologist that wants to show fibrin in lung. She wants also black nuclei and we tried a Movat's to look at fibrin. The fibrin in the lung is showing up light blue not red! Any suggestions on how to show fibrin distinctly, with black nuclei? thanks! Judy ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 69, Issue 9 *************************************** From JCBRITTON <@t> Cheshire-Med.COM Wed Aug 12 07:40:58 2009 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Wed Aug 12 07:41:05 2009 Subject: [Histonet] LYVE-1 antibody In-Reply-To: <61135F0455D33347B5AAE209B903A30429D3500A@EXCHVS2.medctr.ad.wfubmc.edu> References: <61135F0455D33347B5AAE209B903A30429D3500A@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: We have one and we love it! Our Pathologists keep adding more and more antibodies to our list. It is so easy to program. Leica's support staff are great too! Josie Britton HT Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Tuesday, August 11, 2009 2:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LYVE-1 antibody Hello, I wanted to know if anyone has tried this antibody on the Bond stainer, and if so, how did it work for you? I have purchased one from Santa Cruz Biotechnology (cat#sc-80170) at the request of one of our Pathologists. Any feedback is appreciated. Thanks! Martha Ward Molecular Diagnostics Lab Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From JCBRITTON <@t> Cheshire-Med.COM Wed Aug 12 07:44:38 2009 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Wed Aug 12 07:44:44 2009 Subject: [Histonet] ISH In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C438E0A2E1FAA@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C438E0A2E1FAA@HPEMX3.HealthPartners.int> Message-ID: Try Novocastra and Bond Reagent products 2009. 800-248-0123 ext. 7880. I hope this helps! Josie Britton HT Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, August 11, 2009 2:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ISH Can anyone supply me with a vendor for Kappa/Lambda ISH? I heard that Dako and Ventana have had to take their ASR Kappa.Lamda product off of the market. I am in need of where to go for ASR or IVD in this area!! Thanks, Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From cmiller <@t> physlab.com Wed Aug 12 08:19:33 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Aug 12 08:19:39 2009 Subject: [Histonet] IHC validations Message-ID: Does anyone have a validation procedure on approved protocols for your IHC? I have a new Ventana Benchmark XT. I have a validation policy and procedure for clinical but it doesn't relate well to IHC. In the past I have written on the protocol and run procedures the date it was approved by a pathologist. Any suggestions would be welcome, Thanks Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From cmiller <@t> physlab.com Wed Aug 12 08:50:01 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Aug 12 08:50:09 2009 Subject: [Histonet] Saturday opening Message-ID: I have an opening for a part time HT working Saturday mornings only (approximately 5-8 hours) anyone interested contact me. Thanks Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From sbreeden <@t> nmda.nmsu.edu Wed Aug 12 08:51:12 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Aug 12 08:51:18 2009 Subject: [Histonet] Stain Disposal Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46A27@nmdamailsvr.nmda.ad.nmsu.edu> Looking for a one-source listing of how to dispose of stains (i.e., specials - carbol fuchsin, iodine, Tartrazine, etc.). Is there such a list or reference? Thanks. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From relia1 <@t> earthlink.net Wed Aug 12 09:41:14 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Aug 12 09:41:19 2009 Subject: [Histonet] RELIA Special Job Alert Brand New Jobs for Supervisors and Managers 8/12/09 Message-ID: Hi Histonetters! I have several brand new exciting opportunities for experienced Managers, and Supervisors in hospital and private lab environments in several locations nationwide. These are some of the premier employers in the United States. The positions are of course full time and permanent. Here are my BRAND NEW JOBS: Pathology Manager ? Portland, OR Histology Supervisor ? Spokane, WA private pathology lab Histology Supervisor ? Spokane, WA hospital environment Histology Supervisor - Los Angeles, CA Night Shift Production Supervisor ? San Antonio, TX If you would like more information or know of someone else who might be interested, please contact me at relia1@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam RELIA is offering a $500 referral bonus for anyone you refer and I place and a $500 hiring bonus if I place you! There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 6 years I have dedicated my practice solely to placing histology professionals like you. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia Follow me at www.twitter.com/pamatrelia From gu.lang <@t> gmx.at Wed Aug 12 10:35:58 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Aug 12 10:36:03 2009 Subject: AW: [Histonet] ISH In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C438E0A2E1FAA@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C438E0A2E1FAA@HPEMX3.HealthPartners.int> Message-ID: <8F2563136BCA48908C918FDB4EEEEEC4@dielangs.at> This company sells kappa/lambda cish reagens for manual performance. http://zytovision.de/distributors.htm Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Webb, Dorothy L Gesendet: Dienstag, 11. August 2009 20:36 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] ISH Can anyone supply me with a vendor for Kappa/Lambda ISH? I heard that Dako and Ventana have had to take their ASR Kappa.Lamda product off of the market. I am in need of where to go for ASR or IVD in this area!! Thanks, Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Aug 12 10:39:33 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Aug 12 10:39:42 2009 Subject: [Histonet] MDM2 and CDK4 antibodies Message-ID: <356AF132A3B74A988113D70CB048774B@dielangs.at> Hi all! I'm looking for anti human MDM2 and CDK4 antibodies for FFPE material. We want them to run on the Ventana Benchmark XT. Please can anybody provide me with some good information or even protocols. Thanks in advance Gudrun Lang Histolab, general hospital Linz Austria From Traczyk7 <@t> aol.com Wed Aug 12 11:46:57 2009 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Wed Aug 12 11:50:12 2009 Subject: [Histonet] Freezing in Hexane Message-ID: I have a procedure that refers to freezing a tissue sample in chilled Hexane. When I look in a chemical catalogue I find there are several formulas listed. Any direction on which one to use would be greatly appreciated. Thank you. Dorothy Dorothy Murphy Traczyk Murphy-Traczyk & Associates LLC PO Box 602 Point Pleasant, NJ 08742 _dorothy@mtahistology.com_ (mailto:dorothy@mtahistology.com) www.mtahistology.com From Eric.Hoy <@t> UTSouthwestern.edu Wed Aug 12 12:17:49 2009 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Wed Aug 12 12:17:55 2009 Subject: [Histonet] Clarification on ASR Message-ID: I know the histonet group can help me with this question. What are the requirements for reporting results when we use an ASR as part of a procedure? I am getting different answers from various people, all of whom seem to know what they are talking about. A reference would be great. Thank you for your help. Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== From CIngles <@t> uwhealth.org Wed Aug 12 13:03:20 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Aug 12 13:03:25 2009 Subject: [Histonet] Slide Brite xylene substitute References: Message-ID: I inherited a couple gallons of slide brite. We have been using it for a month or two and is very inferior to other clearants we have tried. I just got sick of the drawbacks and got rid of it. It always seemed to leave water droplets on our slides and takes alot longer to clear. We changed back to Propar from Anatech. We have never had problems with it. We still use Permount on our slides but Anatech also has their Refrax mountant that is formulated to work better with Permount. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of jennifer cresor mike hough Sent: Tue 8/11/2009 9:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Brite xylene substitute Hello all, Does anyone out there have experiences with Slide Brite xylene substitute? Did you have any problems? Lately, I have been finding what appears to be water on my slides. Under the microscope it looks like pink gobules on the slide and the tissue. The last 2 days I changed out all of my stains and reagents to see if that would cure the problem and it did not. It is very random, not on all of the slides. But, when these gobules are on the slide it changes the stain dramatically to a dark blue appearing hematoxylin. Any thoughts? I am considering whether to change to a different xylene substitute. Any recommendations. Thank you for your response. Jennifer Temecula, California jencres@ca.rr.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed Aug 12 13:36:15 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Aug 12 13:33:23 2009 Subject: [Histonet] quality control Message-ID: <5A2BD13465E061429D6455C8D6B40E390896BA2694@IBMB7Exchange.digestivespecialists.com> Does anyone keep a list of lot number and expiration dates on all reagents used and what cases they were used with in routine H&E stains and special stains? Thanks, Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com From BBranton <@t> sarapath.com Wed Aug 12 19:01:37 2009 From: BBranton <@t> sarapath.com (Brian Branton) Date: Wed Aug 12 19:04:57 2009 Subject: [Histonet] (no subject) Message-ID: Histonet, Please unsubscribe me, I will be on vacation Brian Branton From Christen <@t> vet.k-state.edu Thu Aug 13 08:08:02 2009 From: Christen <@t> vet.k-state.edu (Shelly Christenson) Date: Thu Aug 13 08:08:45 2009 Subject: [Histonet] Stain Disposal In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46A27@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46A27@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4A83C9E1.EF61.003F.0@vet.k-state.edu> Sally, the "Hazardous Materials in the Histopathology Laboratory " Regulations,, Risks, Handling and Disposal is a nice reference book to have. It can be purchased from Anatech LTD 1-800-ANATECH Shelly Christenson HT(ASCP) Veterinary Diagnostic Lab. Kansas State University 1800 Denison Ave Manhattan Ks 66506 >>> "Breeden, Sara" 8/12/2009 8:51 AM >>> Looking for a one-source listing of how to dispose of stains (i.e., specials - carbol fuchsin, iodine, Tartrazine, etc.). Is there such a list or reference? Thanks. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katelin <@t> cuttingedgehistology.com Thu Aug 13 09:24:57 2009 From: katelin <@t> cuttingedgehistology.com (Katelin Lester) Date: Thu Aug 13 09:25:06 2009 Subject: [Histonet] optimal IHC thickness Message-ID: <4D833A49695A4DC883E86E2F52935373@Front> Hi everyone, I am staining lymph nodes for staging of breast cancer with CKAE1/AE3 and would like to know what everyone is cutting this type of slide at. I searched the archives and my understanding is that, for IHC, how thick the sections are cut is not necessarily critical. I typically cut all IHC at 4?m and I want to achieve the best results possible. Also, on a side note, the Data Sheet suggests using Trypsin, but I have been using Pepsin. Any comments or suggestions about time, quality of Trypsin vs. Pepsin? Thank you, Katelin Katelin Lester Cutting Edge Histology Services, LLC (503) 443-2157 From dellav <@t> musc.edu Thu Aug 13 10:01:29 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Aug 13 10:01:33 2009 Subject: [Histonet] Freezing in Hexane In-Reply-To: References: Message-ID: Hi Dorothy, I've not used hexane. I think 2-methly butane is more commonly used if you are, as I suspect, snap freezing in a solvent chilled in liquid nitrogen. The solvent just needs to have a low freezing point. 2-methyl butane freezes at about - 150 degrees C. I do not know if hexane has a lower freezing point but you can determine that with an internet search. My point in all of this is that you may have other choices for snap freezing if you can't get an answer to your original question re hexane as long as you are able to freeze the needed temperature. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Traczyk7@aol.com Sent: Wednesday, August 12, 2009 12:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freezing in Hexane I have a procedure that refers to freezing a tissue sample in chilled Hexane. When I look in a chemical catalogue I find there are several formulas listed. Any direction on which one to use would be greatly appreciated. Thank you. Dorothy Dorothy Murphy Traczyk Murphy-Traczyk & Associates LLC PO Box 602 Point Pleasant, NJ 08742 _dorothy@mtahistology.com_ (mailto:dorothy@mtahistology.com) www.mtahistology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Aug 13 10:23:51 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 13 10:23:56 2009 Subject: [Histonet] optimal IHC thickness In-Reply-To: <4D833A49695A4DC883E86E2F52935373@Front> Message-ID: <621313.6681.qm@web65707.mail.ac4.yahoo.com> I always used 3-4?m and prefer HIER over enzymes. Ren? J. --- On Thu, 8/13/09, Katelin Lester wrote: From: Katelin Lester Subject: [Histonet] optimal IHC thickness To: histonet@lists.utsouthwestern.edu Date: Thursday, August 13, 2009, 10:24 AM Hi everyone, I am staining lymph nodes for staging of breast cancer with CKAE1/AE3 and would like to know what everyone is cutting this type of slide at. I searched the archives and my understanding is that, for IHC, how thick the sections are cut is not necessarily critical.? I typically cut all IHC at 4?m and I want to achieve the best results possible. Also, on a side note, the Data Sheet suggests using Trypsin, but I have been using Pepsin. Any comments or suggestions about time, quality of Trypsin vs. Pepsin? Thank you, Katelin Katelin Lester Cutting Edge Histology Services, LLC (503) 443-2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From weihsianghuang <@t> gmail.com Thu Aug 13 13:27:54 2009 From: weihsianghuang <@t> gmail.com (Wei-Hsiang Huang) Date: Thu Aug 13 13:27:57 2009 Subject: [Histonet] advice about bone section please Message-ID: Hi all, I'm planning to do paraffin embed section on mouse joint (E12~P10) to detect the GFP-tagged protein expression. Since bone has autofluorescence, I'm wondering whether IHC would be a good choice for postnatal bone, and can I do immunofluorescence on joint in E12~16 mouse without having autofluorescence effect? I've tried cryosection (10 micrometer) but the bone detached and doesn't give me good morphology so I decided to try paraffin. thank you all in advance. W From dmnelson <@t> iastate.edu Thu Aug 13 14:22:46 2009 From: dmnelson <@t> iastate.edu (Diane Gerjets) Date: Thu Aug 13 14:22:50 2009 Subject: [Histonet] Picric Acid Message-ID: <200908131922.n7DJMjno003368@despam-10.iastate.edu> Hello everyone! I would like to know what all of you are doing now that picric acid is so hard to get? We use it for bouin's, trichrome and gram stain. I know we can buy these already made up, but we wanted to know if there might be other alternatives. I'll be off tomorrow (state fair time), but will check my e-mail first thing monday morning. Thank-you in advance for all your help! Diane From rsrichmond <@t> gmail.com Thu Aug 13 14:40:01 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Thu Aug 13 14:40:05 2009 Subject: [Histonet] Re: Freezing in Hexane Message-ID: Dorothy Murphy Traczyk at Murphy-Traczyk & Associates in Point Pleasant, NJ asks about using hexane for snap-freezing. I wouldn't want any of the hexanes (there are several isomers) or 2-methylbutane around my lab because of the explosion hazard if I could avoid it. Look up 3M Novec Engineered Fluid HFE-7100 (methyl nonafluoroisobutyl ether) in the Histonet archives (accessible through Google). Probably usable for this purpose, and not flammable. If you do this, please report your experience to Histonet - several of us on here are interested. Bob Richmond Samurai Pathologist Knoxville TN From sraibley <@t> yahoo.com Thu Aug 13 14:18:07 2009 From: sraibley <@t> yahoo.com (Susan Raibley) Date: Thu Aug 13 14:44:51 2009 Subject: [Histonet] Specimen Storage Message-ID: <66048.83797.qm@web56007.mail.re3.yahoo.com> I work?for a research company and am looking?at ideas for storage of specimens. We currently use Bitran bags?to?keep the extra parts with some formalin and then?heat seal them in the Kapak heat seal bags.?The Bitran?bags are getting so expensive and tend to leak. Does anyone have a better system for long term storage of multiple animal specimens? Thanks! ? Susan Bincsik, HT (ASCP) __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From ndevans <@t> stanford.edu Thu Aug 13 14:50:20 2009 From: ndevans <@t> stanford.edu (Nicholas David Evans) Date: Thu Aug 13 14:50:24 2009 Subject: [Histonet] X-gal staining In-Reply-To: References: Message-ID: <6733845659A84A0D85D03EF7D05BFB32@DellDesktop2> Hello, I was wondering whether anyone might advise me on X-gal staining. I am looking at the expression of LacZ knocked into the locus of our gene of interest. We cryoembed, take sections and stain for beta-galactosidase activity using X-gal. The blue stain is often punctuate and noticeably limited to the nucleus, whereas in other areas the staining is diffuse and appears throughout the cytoplasm of the cells. I have guessed that this may be related to the level of expression; i.e. that in cells with a low reporter activity beta-gal is limited to the nucleus, but that at higher expression the whole cell becomes riddled with it. If anyone knows anything about the subcellular distribution/localisation of beta-gal in mammalian cells, or can direct me to some good literature I would be grateful. Best wishes Nick From rjbuesa <@t> yahoo.com Thu Aug 13 15:55:50 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 13 15:55:55 2009 Subject: [Histonet] Specimen Storage In-Reply-To: <66048.83797.qm@web56007.mail.re3.yahoo.com> Message-ID: <857506.6350.qm@web65704.mail.ac4.yahoo.com> We used to store them directly in the sealed?Kapak pouches. I believe there are some "for home" commercial alternatives, although probably for frozen storage. Worth exploring "out of the lab" alternatives. Ren? J. --- On Thu, 8/13/09, Susan Raibley wrote: From: Susan Raibley Subject: [Histonet] Specimen Storage To: histonet@lists.utsouthwestern.edu Date: Thursday, August 13, 2009, 3:18 PM I work?for a research company and am looking?at ideas for storage of specimens. We currently use Bitran bags?to?keep the extra parts with some formalin and then?heat seal them in the Kapak heat seal bags.?The Bitran?bags are getting so expensive and tend to leak. Does anyone have a better system for long term storage of multiple animal specimens? Thanks! ? Susan Bincsik, HT (ASCP) __________________________________________________ Do You Yahoo!? Tired of spam?? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Laura.Miller <@t> leica-microsystems.com Thu Aug 13 16:01:50 2009 From: Laura.Miller <@t> leica-microsystems.com (Laura.Miller@leica-microsystems.com) Date: Thu Aug 13 16:02:00 2009 Subject: [Histonet] Laura Miller is on vacation. Message-ID: I will be out of the office starting 08/13/2009 and will not return until 08/17/2009. I will respond to your email on Monday. Thanks! ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From barb <@t> medialabinc.net Thu Aug 13 16:47:48 2009 From: barb <@t> medialabinc.net (Barbara Cebulski) Date: Thu Aug 13 16:48:12 2009 Subject: [Histonet] job opportunity for technologist interested in development of educational materials Message-ID: This is a great opportunity for someone with expertise in laboratory medicine and in education. MediaLabInc. (http://www.medialabinc.net), located in metro Atlanta, GA, is a leading provider of online compliance and training software for the clinical laboratory industry. We presently deliver over 300,000 online course events to individuals and institutions annually, and our customer base continues to expand, as does the depth, breath, and quality of our course offerings. We are seeking an additional medical technologist (MT) to join our team. Experience as a classroom or online educator and experience in writing technical or educational materials is essential. Sub-speciality training, experience in laboratory administration, safety, or compliance would be helpful. Knowledge or experience in instructional design, digital photography, photomicroscopy, and video production will also be considered. We realize that no one individual will have all these skills, but a strong background as a clinical laboratorian and educator is essential, and all interested MT applicants with at least 5 years of laboratory and/or educational experience are strongly encouraged to apply. The successful candidate will work closely with our team to help us to develop and update courseware that continues to meet the ever changing needs of clinical laboratories. He or she will also work with other contributing course authors and local laboratory managers and technologists. Pay and benefits are negotiable and highly competitive. Work from home several days per week, and enjoy working in our comfortable office in suburban Atlanta the rest of the time. Some opportunity for travel is available. Preference will be given to residents of metro Atlanta, but we will consider an outstanding candidate from the United States or Canada who is willing to relocate. We will also consider part-time employment for the right candidate who wants to work from his / her home location in the U.S. or Canada. Please send your CV to Paul Fekete, MD, via email attachment to fekete@gmail.com. For more information about MediaLab, visit http://www.medialabinc.net. From histonetalias <@t> gmail.com Thu Aug 13 18:35:46 2009 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Thu Aug 13 18:35:50 2009 Subject: [Histonet] Negative Staining Positive Message-ID: <4b6c85510908131635o328b4625pef794bc566d43294@mail.gmail.com> Has anyone noticed their negative mouse having some positive staining using the Ventana Benchmark XT? We use the iview detection. Any ideas? Thank you. -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States From akemiat3377 <@t> yahoo.com Thu Aug 13 20:44:56 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Aug 13 20:44:59 2009 Subject: [Histonet] NSH Meeting Hotels Message-ID: <309098.58564.qm@web31304.mail.mud.yahoo.com> Hi All, Anyone trying to reserve a room at the Doubletree should read this. I just tried to reserve a room for the upcoming Symposium.? I went to the NSH website and looked under the "Hotel & Travel" icon and saw that the only hotel that wasn't Sold Out was the Double Tree.? I called and they never heard of us "NSH". I read all the information to the reservation person and she came up with a blank.? I requested the supervisor.? After searching for about 15 minutes, she found us under histotechnology.? Anyone trying to book a room should state that our GROUP CODE is "CI0N" Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com From jqb7 <@t> cdc.gov Fri Aug 14 04:45:47 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Aug 14 04:46:21 2009 Subject: [Histonet] NSH Meeting Hotels In-Reply-To: <309098.58564.qm@web31304.mail.mud.yahoo.com> References: <309098.58564.qm@web31304.mail.mud.yahoo.com> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE101AD3559@LTA3VS011.ees.hhs.gov> And when I made my reservation at the Sheraton the NSH rate they had was more expensive than what the NSH website showed. Not much, but it was higher. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, August 13, 2009 9:45 PM To: histonet Subject: [Histonet] NSH Meeting Hotels Hi All, Anyone trying to reserve a room at the Doubletree should read this. I just tried to reserve a room for the upcoming Symposium.? I went to the NSH website and looked under the "Hotel & Travel" icon and saw that the only hotel that wasn't Sold Out was the Double Tree.? I called and they never heard of us "NSH". I read all the information to the reservation person and she came up with a blank.? I requested the supervisor.? After searching for about 15 minutes, she found us under histotechnology.? Anyone trying to book a room should state that our GROUP CODE is "CI0N" Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine-walters <@t> uiowa.edu Fri Aug 14 08:05:48 2009 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Fri Aug 14 08:06:00 2009 Subject: [Histonet] NSH Meeting Hotels In-Reply-To: <309098.58564.qm@web31304.mail.mud.yahoo.com> References: <309098.58564.qm@web31304.mail.mud.yahoo.com> Message-ID: Good to know, but when I booked they had no trouble finding "National Society of Histotechnology". I'd've never come up with "CION"!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, August 13, 2009 8:45 PM To: histonet Subject: [Histonet] NSH Meeting Hotels Hi All, Anyone trying to reserve a room at the Doubletree should read this. I just tried to reserve a room for the upcoming Symposium.? I went to the NSH website and looked under the "Hotel & Travel" icon and saw that the only hotel that wasn't Sold Out was the Double Tree.? I called and they never heard of us "NSH". I read all the information to the reservation person and she came up with a blank.? I requested the supervisor.? After searching for about 15 minutes, she found us under histotechnology.? Anyone trying to book a room should state that our GROUP CODE is "CI0N" Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcampbell <@t> vdxpathology.com Fri Aug 14 09:12:52 2009 From: jcampbell <@t> vdxpathology.com (Jennifer Campbell) Date: Fri Aug 14 09:12:56 2009 Subject: [Histonet] destaining cyto slides for immuno Message-ID: <5658CBDB9EAE6545ABE50D2563D81BF8261F35@VDXSERVER01.vdxpathology.local> Hi All, How would you go about destaining a cyto slide and running a CKAE1/AE3? I was told that acid alcohol to remove the stain beforehand may not be necessary as heat retrieval may remove it adequately enough. Does anyone have any recommendations? Thanks, Jen From LSebree <@t> uwhealth.org Fri Aug 14 09:24:54 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Aug 14 09:25:00 2009 Subject: [Histonet] destaining cyto slides for immuno In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF8261F35@VDXSERVER01.vdxpathology.local> Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A5890357065A@UWHC-MAIL01.uwhis.hosp.wisc.edu> We used to destain but after a while decided that whatever remaining PAP stain would not interfere with interpretation...most of it is gone after IHC anyway. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Friday, August 14, 2009 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] destaining cyto slides for immuno Hi All, How would you go about destaining a cyto slide and running a CKAE1/AE3? I was told that acid alcohol to remove the stain beforehand may not be necessary as heat retrieval may remove it adequately enough. Does anyone have any recommendations? Thanks, Jen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Aug 14 09:27:54 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Aug 14 09:28:03 2009 Subject: AW: [Histonet] destaining cyto slides for immuno In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF8261F35@VDXSERVER01.vdxpathology.local> References: <5658CBDB9EAE6545ABE50D2563D81BF8261F35@VDXSERVER01.vdxpathology.local> Message-ID: We do p16-stain on PAP-smears on the Ventana Benchmark. The retrieval step removes the dye, so there's no extra procedure needed. PAP smears are not formalin fixed specimen, but a mild antigen retrieval improves the result and destains the slides. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jennifer Campbell Gesendet: Freitag, 14. August 2009 16:13 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] destaining cyto slides for immuno Hi All, How would you go about destaining a cyto slide and running a CKAE1/AE3? I was told that acid alcohol to remove the stain beforehand may not be necessary as heat retrieval may remove it adequately enough. Does anyone have any recommendations? Thanks, Jen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From igor.deyneko <@t> gmail.com Fri Aug 14 09:29:06 2009 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Fri Aug 14 09:29:09 2009 Subject: [Histonet] HOPE Fixation technique Message-ID: <35e16a770908140729v5d934850r60bf1fe188d5ffe9@mail.gmail.com> Dear Histonetters! Recently I cam across this new product offered by Polysciences, called HOPE Fixation( Hepes-glutamic acid buffer mediated Organic solvent Protection Effect). It claims that there is no retreival necessary for IHC and nor formalin either and that it makes even the hardest antibodies to work.So I was wondering if anyone out there has actually tried it out yet. Thank you. Sincerely, Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 From rjbuesa <@t> yahoo.com Fri Aug 14 10:00:54 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 14 10:00:58 2009 Subject: [Histonet] destaining cyto slides for immuno In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF8261F35@VDXSERVER01.vdxpathology.local> Message-ID: <922824.4190.qm@web65716.mail.ac4.yahoo.com> Jennifer: Heat retrieval will not destain the smear. Use acid alcohol followed by alcohol and water and then do the IHC procedure. You will NOT need HIER unless your smears were fixed with formalin. Since they usually are fixed only with alcohol, you can go directly to the IHC procedure. Ren? J. --- On Fri, 8/14/09, Jennifer Campbell wrote: From: Jennifer Campbell Subject: [Histonet] destaining cyto slides for immuno To: histonet@lists.utsouthwestern.edu Date: Friday, August 14, 2009, 10:12 AM Hi All, ? How would you go about destaining a cyto slide and running a CKAE1/AE3?? I was told that acid alcohol to remove the stain beforehand may not be necessary as heat retrieval may remove it adequately enough. Does anyone have any recommendations? Thanks, Jen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KGroeger <@t> USLABS.net Fri Aug 14 10:12:15 2009 From: KGroeger <@t> USLABS.net (Karin Groeger) Date: Fri Aug 14 10:12:23 2009 Subject: [Histonet] destaining cyto slides for immuno In-Reply-To: References: <5658CBDB9EAE6545ABE50D2563D81BF8261F35@VDXSERVER01.vdxpathology.local> Message-ID: We do the same at USLabs. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, August 14, 2009 7:28 AM To: 'Jennifer Campbell' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] destaining cyto slides for immuno We do p16-stain on PAP-smears on the Ventana Benchmark. The retrieval step removes the dye, so there's no extra procedure needed. PAP smears are not formalin fixed specimen, but a mild antigen retrieval improves the result and destains the slides. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jennifer Campbell Gesendet: Freitag, 14. August 2009 16:13 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] destaining cyto slides for immuno Hi All, How would you go about destaining a cyto slide and running a CKAE1/AE3? I was told that acid alcohol to remove the stain beforehand may not be necessary as heat retrieval may remove it adequately enough. Does anyone have any recommendations? Thanks, Jen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. From emerald_lake77 <@t> yahoo.com Fri Aug 14 10:37:45 2009 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Fri Aug 14 10:37:50 2009 Subject: [Histonet] Can DAB block an epitope?? Message-ID: <661810.77228.qm@web110611.mail.gq1.yahoo.com> Hello all, ? I was hoping to run a chromogenic immuno to check if two proteins colocalize.? ? Since both antibodies are made in rabbit, I was going to stain one and visualize with DAB and then heat retrieve (pressure or steam) to drop off the first antibody and restain with my second rabbit antibody using a different color to visualize. ? How likely, if at all will the DAB precipitate block / mask the epitope of my second antibody if indeed they are in a similar location? ? Any info would be greatly appreciated. ? Thank you! ? Regards. ? Gustave ? Gustave T. Hebert Research Scientist I Metabolic Disease Research Wyeth Research 200 CambridgePark Drive Cambridge, MA 02140 From elainemonroe <@t> live.com Fri Aug 14 10:53:09 2009 From: elainemonroe <@t> live.com (nancy monroe) Date: Fri Aug 14 10:53:13 2009 Subject: [Histonet] (no subject) Message-ID: I have the need to reprocess some tissue. Does anyone have a procedure that I can follow? The tissue is breast and lymph nodes. Thank you, Elaine Monroe _________________________________________________________________ Get back to school stuff for them and cashback for you. http://www.bing.com/cashback?form=MSHYCB&publ=WLHMTAG&crea=TEXT_MSHYCB_BackToSchool_Cashback_BTSCashback_1x1 From elainemonroe <@t> live.com Fri Aug 14 10:58:45 2009 From: elainemonroe <@t> live.com (nancy monroe) Date: Fri Aug 14 10:58:49 2009 Subject: [Histonet] reprocessing tissue Message-ID: Does anyone have a procedure for reprocessing tissue? We need to reprocess a breast and lymph node sections. They are squishy in the middle. They were probably cut to thick and not fixed long enough but you know the Pathologist wants them as soon as possible. Any ideas will be appreciated. Thank you, Elaine Monroe MLT(ASCP)HT Spring Memorial Hospital _________________________________________________________________ Get your vacation photos on your phone! http://windowsliveformobile.com/en-us/photos/default.aspx?&OCID=0809TL-HM From TMcNemar <@t> lmhealth.org Fri Aug 14 11:22:16 2009 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Aug 14 11:22:35 2009 Subject: [Histonet] reprocessing tissue In-Reply-To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E123@lmhsmail.lmhealth.org> You can run them through the cleaning cycle on your processor then just process normally. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of nancy monroe Sent: Friday, August 14, 2009 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] reprocessing tissue Does anyone have a procedure for reprocessing tissue? We need to reprocess a breast and lymph node sections. They are squishy in the middle. They were probably cut to thick and not fixed long enough but you know the Pathologist wants them as soon as possible. Any ideas will be appreciated. Thank you, Elaine Monroe MLT(ASCP)HT Spring Memorial Hospital _________________________________________________________________ Get your vacation photos on your phone! http://windowsliveformobile.com/en-us/photos/default.aspx?&OCID=0809TL-HM_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From king.laurie <@t> marshfieldclinic.org Fri Aug 14 11:28:07 2009 From: king.laurie <@t> marshfieldclinic.org (King, Laurie) Date: Fri Aug 14 11:28:15 2009 Subject: [Histonet] reprocessing tissue Message-ID: <200908141628.n7EGSAf5029135@mailhost2.mfldclin.edu> Just melt the paraffin off and pat dry, process from formalin normally. Just did it yesterday, works great. Laurie ------Original Message------ From: "nancy monroe" Date: Fri Aug 14, 2009 -- 10:59:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] reprocessing tissue Does anyone have a procedure for reprocessing tissue? We need to reprocess a breast and lymph node sections. They are squishy in the middle. They were probably cut to thick and not fixed long enough but you know the Pathologist wants them as soon as possible. Any ideas will be appreciated. Thank you, Elaine Monroe MLT(ASCP)HT Spring Memorial Hospital _________________________________________________________________ Get your vacation photos on your phone! http://windowsliveformobile.com/en-us/photos/default.aspx?&OCID=0809TL-HM_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Fri Aug 14 11:33:21 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Aug 14 11:33:27 2009 Subject: [Histonet] Melanin bleach Message-ID: <57BE698966D5C54EAE8612E8941D7683064CF498@EXCHANGE3.huntingtonhospital.com> I need to do a melanin bleach with potassium permanganate and oxalic acid. I do not have any oxalic acid. Does anyone know of a substitute for the oxalic acid? If I recall correctly, the ox acid gets rid of the pot permang. If I rinse long enough in water, will that work???? Laurie Colbert From rjbuesa <@t> yahoo.com Fri Aug 14 11:53:58 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 14 11:54:05 2009 Subject: [Histonet] reprocessing tissue In-Reply-To: Message-ID: <443421.88771.qm@web65716.mail.ac4.yahoo.com> Put you tissues in the tissue processor through the cleaning cycle, and reprocess them again starting in the alcohols. Ren? J. --- On Fri, 8/14/09, nancy monroe wrote: From: nancy monroe Subject: [Histonet] reprocessing tissue To: histonet@lists.utsouthwestern.edu Date: Friday, August 14, 2009, 11:58 AM Does anyone have a procedure for reprocessing tissue? We need to reprocess a breast and lymph node sections. They are squishy in the middle. They were probably cut to thick and not fixed long enough but you know the Pathologist wants them as soon as possible. Any ideas will be appreciated. Thank you, Elaine Monroe MLT(ASCP)HT Spring Memorial Hospital _________________________________________________________________ Get your vacation photos on your phone! http://windowsliveformobile.com/en-us/photos/default.aspx?&OCID=0809TL-HM_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 14 11:55:58 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 14 11:56:03 2009 Subject: [Histonet] Can DAB block an epitope?? In-Reply-To: <661810.77228.qm@web110611.mail.gq1.yahoo.com> Message-ID: <65840.93612.qm@web65707.mail.ac4.yahoo.com> Theoretically, and depending on the reaction specificity, DAB should not block the epitope. Ren? J. --- On Fri, 8/14/09, GT Hebert wrote: From: GT Hebert Subject: [Histonet] Can DAB block an epitope?? To: Histonet@lists.utsouthwestern.edu Date: Friday, August 14, 2009, 11:37 AM Hello all, ? I was hoping to run a chromogenic immuno to check if two proteins colocalize.? ? Since both antibodies are made in rabbit, I was going to stain one and visualize with DAB and then heat retrieve (pressure or steam) to drop off the first antibody and restain with my second rabbit antibody using a different color to visualize. ? How likely, if at all will the DAB precipitate block / mask the epitope of my second antibody if indeed they are in a similar location? ? Any info would be greatly appreciated. ? Thank you! ? Regards. ? Gustave ? Gustave T. Hebert Research Scientist I Metabolic Disease Research Wyeth Research 200 CambridgePark Drive Cambridge, MA 02140 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kcai <@t> prosci-inc.com Fri Aug 14 11:56:56 2009 From: kcai <@t> prosci-inc.com (Karen Cai) Date: Fri Aug 14 11:57:07 2009 Subject: [Histonet] Need help on Streptavidin-HRP conjugate for IHC Message-ID: <000701ca1d00$3b8b7080$7a01a8c0@mic> Hi, I am using ABC kit for DAB-IHC experiment. But somehow, I don't feel it is a good choice since the background is kinda high. Is there anybody having some suggestions? Which kind of steptavidin-HRP are you using for DAB-IHC? From which company? Thank you very much for your kind reply, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com From making <@t> ufl.edu Fri Aug 14 12:16:40 2009 From: making <@t> ufl.edu (MKing) Date: Fri Aug 14 12:16:49 2009 Subject: [Histonet] streptavidin peroxidase Message-ID: <4A859BF8.5010700@ufl.edu> Sigma E-2886 extravidin peroxidase, 1:1000 in 0.1 M PBS or sodium acetate on almost every IHC variation (fixation, species, primary) we do. Supposed to be less nonspecific binding than streptavidin. Mike King UF Pharmacology & Therapeutics ------------------------------ Message: 27 Date: Fri, 14 Aug 2009 09:56:56 -0700 From: "Karen Cai" Subject: [Histonet] Need help on Streptavidin-HRP conjugate for IHC To: Message-ID: <000701ca1d00$3b8b7080$7a01a8c0@mic> Content-Type: text/plain; charset="us-ascii" Hi, I am using ABC kit for DAB-IHC experiment. But somehow, I don't feel it is a good choice since the background is kinda high. Is there anybody having some suggestions? Which kind of steptavidin-HRP are you using for DAB-IHC? From which company? Thank you very much for your kind reply, Best, Karen Karen Cai Research Scientist Prosci Incorporated (858) 513-2638 x 204 (858) 513-2692 Fax www.prosci-inc.com From MLunetta <@t> luhcares.org Fri Aug 14 15:02:37 2009 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Fri Aug 14 15:03:26 2009 Subject: [Histonet] HT opening in Colorado Message-ID: <4A856E7D020000A80003512E@mail.luhcares.org> Hello Histo-Netters, We have an HT job opening here in Longmont Colorado, located north of Denver and just NE of Boulder. Please apply through the website. I have attached the link. All new and experianced HT's please apply. Also please add my name to your application so we can track the responces from histo net. http://luh.jobscience.com/JsrApp/index.cfm?cmd=showPositionDetail&nextEvent=doSearchPositions&cobrandId=9000&masterId=luh001&accountId=9F3D0B3C-E71A-17E7-69B649D49CA1C88F&positionId=511568&urlArgList=c2VhcmNoVHlwZT1xdWljayZzdGFydD0xJmNvdW50PTUwJmRlcHRJZD0mam9iSWQ9JmtleXdvcmQ9&bid=1297 If the link does not work the web site is www.luhcares.org Thanks Matt Lunetta BS, HT (ASCP) From denise.woodward <@t> uconn.edu Fri Aug 14 15:20:34 2009 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Fri Aug 14 15:20:40 2009 Subject: [Histonet] unsubscribe Message-ID: From kblack <@t> digestivehlth.com Fri Aug 14 15:27:50 2009 From: kblack <@t> digestivehlth.com (Konni Black) Date: Fri Aug 14 15:27:59 2009 Subject: [Histonet] Job Opening Message-ID: Hello All, There is a new histology lab opening in St. Augustine, Florida. Beautiful area on the Palm Coast. Great pay and working conditions. Extremely flexible hours. In fact, create your own schedule! Contact me for details or send resume to kblack@digestivelth.com Don't miss this ground floor opportunity. Konni Black From tfraser <@t> olympicmedical.org Fri Aug 14 16:47:54 2009 From: tfraser <@t> olympicmedical.org (Tasha Fraser) Date: Fri Aug 14 16:48:02 2009 Subject: [Histonet] Shandon Excelsior Tissue Processor Message-ID: <01A05F75D299414EBFFB912EF6DAF66902C74029@is-210vs.olympicmedical.local> Would love to get some feedback hopefully both good and bad in regards to the Shandon Excelsior Tissue Processor. Looking to purchase a new tissue processor and I already have a Tissue-Tek VIP 5. I'm going to Demo a Shandon Excelsior and probably a Leica ASP300 S. I do love the Tissue-Tek VIP's, but don't feel the need to demo the VIP6. Any feedback on any of these processors would be greatly appreciated! Tasha Fraser, HT (ASCP) Olympic Medical Center Port Angeles, WA ------------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. From amosbrooks <@t> gmail.com Fri Aug 14 17:29:25 2009 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Aug 14 17:29:33 2009 Subject: [Histonet] Can DAB block an epitope?? Message-ID: <582736990908141529r82cfc3ftb45a27fff37c2eba@mail.gmail.com> Hi Gustave, I do this frequently, and it works great with some caveats. First it is best to use a *very* red chromogen (if you choose to use red). I have not had a lot of luck with AEC the fast red in the DAKO alk phos kit looks great against DAB. Colocalization of antigens can be tricky. In this case immunofluoresfence really is the way to go. Biocare Medical has some great Fluorescent secondaries. Colocalized chromogens just look muddy and not very good. The DAB doesn't really 'block' the epitope, but ut does make it a heck of a lot harder to see. Good Luck, Amos Message: 19 Date: Fri, 14 Aug 2009 08:37:45 -0700 (PDT) From: GT Hebert Subject: [Histonet] Can DAB block an epitope?? To: Histonet@lists.utsouthwestern.edu Message-ID: <661810.77228.qm@web110611.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello all, I was hoping to run a chromogenic immuno to check if two proteins colocalize. Since both antibodies are made in rabbit, I was going to stain one and visualize with DAB and then heat retrieve (pressure or steam) to drop off the first antibody and restain with my second rabbit antibody using a different color to visualize. How likely, if at all will the DAB precipitate block / mask the epitope of my second antibody if indeed they are in a similar location? Any info would be greatly appreciated. Thank you! Regards. Gustave Gustave T. Hebert Research Scientist I Metabolic Disease Research Wyeth Research 200 CambridgePark Drive Cambridge, MA 02140 From sotlak <@t> yahoo.gr Sun Aug 16 04:47:13 2009 From: sotlak <@t> yahoo.gr (sotiris lakis) Date: Sun Aug 16 04:47:19 2009 Subject: [Histonet] CD31(PECAM-1) by novocastra on FFPE rabbit tissue Message-ID: <702299.82182.qm@web23004.mail.ird.yahoo.com> Hi, I have question about CD31(PECAM-1) by novocastra. Has anybody worked with it on FFPE rabbit tissue? I know for instance that DAKO's works, but could find no references for the one by novocastra. Moreover I am using a Bond protocol that comes with an antimouse/antirabbit IgG 2nd Ab. I could also use some suggestions about a good blocking reagent for rabbit. Company tells me I need another detection system but that's not an option...So any protocol manipulation ideas are more than wellcome!? Thank you in advance Sotiris Lakis Department of Pathology G.Gennimatas Community Hospital Thessaloniki 54635 Hellas (Greece) ? ? ___________________________________________________________ ?????????????? Yahoo!; ?????????? ?? ?????????? ???????? (spam); ?? Yahoo! Mail ???????? ??? ???????? ?????? ????????? ???? ??? ??????????? ????????? http://login.yahoo.com/config/mail?.intl=gr From AnthonyH <@t> chw.edu.au Sun Aug 16 18:47:53 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 16 18:48:12 2009 Subject: [Histonet] destaining cyto slides for immuno In-Reply-To: <5658CBDB9EAE6545ABE50D2563D81BF8261F35@VDXSERVER01.vdxpathology.local> Message-ID: Jen, I would assume that the cyto slide was either air-dried or ethanol fixed. You do not need to heat retrieve nor enzyme treat. Just remove the coverslip, (give a good soak in xylene or equivalent to remove all the mountant), re-hydrate to water, block endogenous peroxidase, add the AE1/AE3 antibody and proceed as usual. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell Sent: Saturday, 15 August 2009 12:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] destaining cyto slides for immuno Hi All, How would you go about destaining a cyto slide and running a CKAE1/AE3? I was told that acid alcohol to remove the stain beforehand may not be necessary as heat retrieval may remove it adequately enough. Does anyone have any recommendations? Thanks, Jen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From jkiernan <@t> uwo.ca Sun Aug 16 23:21:04 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sun Aug 16 23:21:08 2009 Subject: [Histonet] X-gal staining Message-ID: Dear ndevans@stanford.edu You may be detecting endogenous animal in addition to bacterial (lac-z) beta-galactosidase activity. Indigogenic methods were recently reviewed in Biotechnic & Histochemistry 82: 72-103 (2007). The pH of the indigogenic substrate solution is important. Many other factors can cause false positive and false negative X-gal staining. Do your homework! John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Nicholas David Evans Date: Thursday, August 13, 2009 15:51 Subject: [Histonet] X-gal staining To: histonet@lists.utsouthwestern.edu > Hello, > > I was wondering whether anyone might advise me on X-gal > staining. > > I am looking at the expression of LacZ knocked into the locus of > our gene > of interest. We cryoembed, take sections and stain for beta- > galactosidaseactivity using X-gal. The blue stain is often > punctuate and noticeably > limited to the nucleus, whereas in other areas the staining is > diffuse and > appears throughout the cytoplasm of the cells. I have guessed > that this > may be related to the level of expression; i.e. that in cells > with a low > reporter activity beta-gal is limited to the nucleus, but that > at higher > expression the whole cell becomes riddled with it. If anyone knows > anything about the subcellular distribution/localisation of beta- > gal in > mammalian cells, or can direct me to some good literature I > would be > grateful. > > Best wishes > Nick > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Mon Aug 17 02:14:27 2009 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Mon Aug 17 02:14:53 2009 Subject: [Histonet] Can DAB block an epitope?? Message-ID: <8a32e66a491bb53e.4a891f73@amc.uva.nl> Herbert and Rene,To my opinion DAB is the only know chromogen reaction product that does have the potential to block an epitope. Afterall, the paper of Sternberger and Joseph (JHC 1979) describes a double staining procedure with two primaries of similar species based on DAB as first chromogen, no elution step or heating step, and subsequent performance of a 2nd IHC procedure. That protocol works perfect as long as DAB is used in the first staining sequence. This double staining procedure is however NOT working with other chromogens. If other chromgens are used, a heating step is required to remove the immuno-reagents from the first staining sequence. My paper in JOH of last year (31:119-127) combines for example two AP procedures (red and blue) for double staining. Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Fri, 14 Aug 2009 09:55:58 -0700 (PDT) From: Rene J Buesa <> Subject: Re: [Histonet] Can DAB block an epitope?? To: Histonet@lists.utsouthwestern.edu, GT Hebert emerald_lake77@yahoo.com Theoretically, and depending on the reaction specificity, DAB should not block the epitope. Ren? J. --- On Fri, 8/14/09, GT Hebert wrote: From: GT Hebert Subject: [Histonet] Can DAB block an epitope?? To: Histonet@lists.utsouthwestern.edu Date: Friday, August 14, 2009, 11:37 AM Hello all, ? I was hoping to run a chromogenic immuno to check if two proteins colocalize.? ? Since both antibodies are made in rabbit, I was going to stain one and visualize with DAB and then heat retrieve (pressure or steam) to drop off the first antibody and restain with my second rabbit antibody using a different color to visualize. ? How likely, if at all will the DAB precipitate block / mask the epitope of my second antibody if indeed they are in a similar location? ? Any info would be greatly appreciated. ? Thank you! ? Regards. ? Gustave ? Gustave T. Hebert Research Scientist I Metabolic Disease Research Wyeth Research 200 CambridgePark Drive Cambridge, MA 02140 From philip_manfre <@t> merck.com Mon Aug 17 09:20:50 2009 From: philip_manfre <@t> merck.com (Manfre, Philip) Date: Mon Aug 17 09:20:55 2009 Subject: [Histonet] RA Lamb Lambwax Substitute Message-ID: Good morning Histonet, Does anybody out there know of a good substitute for or have a supply of RA Lamb's Lambwax paraffin? RA Lamb was bought by Thermo and Lambwax is no longer produced. We used Lambwax in our lab for a particular sectioning protocol. It allowed for sectioning of small tissue without removing, soaking, or chilling of the block. This helped keep the alignment of the block to the knife intact over a large number of serial sections. If anybody has experience with other paraffins that can offer the qualities I described or know of a supplier that may have Lambwax, please let me know. T-565 and T-555 are not suitable for this specific technique in our experience. Thanks in advance, Phil. Philip Manfre, BA, HT(ASCP) Senior Research Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From pinetree110567 <@t> yahoo.com Mon Aug 17 12:40:01 2009 From: pinetree110567 <@t> yahoo.com (Jack Cates) Date: Mon Aug 17 12:40:07 2009 Subject: [Histonet] RA Lamb Lambwax Substitute Message-ID: <332196.19228.qm@web110806.mail.gq1.yahoo.com> Philip, I would recommend trying Jerry Helisek with US Laboratory Supplies. He has been able to supply plenty of the RA Lamb items we purchased before they merged with Thermo. his contact information is: jerry@uslabsupplies.com 919-264-7964 ------------------------------ Message: 4 Date: Mon, 17 Aug 2009 10:20:50 -0400 From: "Manfre, Philip" Subject: [Histonet] RA Lamb Lambwax Substitute To: Message-ID: Content-Type: text/plain; charset="us-ascii" Good morning Histonet, Does anybody out there know of a good substitute for or have a supply of RA Lamb's Lambwax paraffin? RA Lamb was bought by Thermo and Lambwax is no longer produced. We used Lambwax in our lab for a particular sectioning protocol. It allowed for sectioning of small tissue without removing, soaking, or chilling of the block. This helped keep the alignment of the block to the knife intact over a large number of serial sections. If anybody has experience with other paraffins that can offer the qualities I described or know of a supplier that may have Lambwax, please let me know. T-565 and T-555 are not suitable for this specific technique in our experience. Thanks in advance, Phil. Philip Manfre, BA, HT(ASCP) Senior Research Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 69, Issue 17 **************************************** From tifei <@t> foxmail.com Mon Aug 17 12:22:51 2009 From: tifei <@t> foxmail.com (TF) Date: Mon Aug 17 14:17:32 2009 Subject: [Histonet] Bielschowsky's Silver Stain for Axons - Message-ID: <200908180122464342728@foxmail.com> Dear All i have a problem in the Bielschowsky staining of axons. I have rat brain tissues, fixed with 4% PFA or 1%PFA+1.25% Glut. With sucrose cryoprotection, cut into 20-25 um sections on a Leica cryostat. I air dried the sections in fume hood for two days before wash in distilled water for silver staining. I have tried several protocols, Bielschowsky staining and several modified ones. But I always get a high background - a large number of black dots appeared on the section without specific signals of fibers. I at first consider that it must be caused of contamination. With careful examination, most of these silver dots are neurons, sometimes showing the morphology of a pyramidal cell in the frontal cortex layer. The whole pattern is like nissle staining - you can visualize the neuronal body, but no fibers. Then I tried another six slides with KMnO4 treatment, then the Oxalic acid prior to the 20% Silver nitrate incubation at 37 C. In two of the slides I observed fine fibers in the white matter - though not strong! But the other four slides are quite similar to before. Can anyone give me some suggestions to improve? To stregnthen the signals I observed, and to make the staining replicable? It seems not be the problem of signal intensity, but none axons are stained in many sections. 2009-08-18 TF From tifei <@t> foxmail.com Mon Aug 17 12:14:19 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Mon Aug 17 14:53:49 2009 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gQ0QzMShQRUNBTS0xKSBieSBub3ZvY2FzdHJhIG9uIEZGUEUgcmFiYml0IHRpc3N1ZQ==?= References: <702299.82182.qm@web23004.mail.ird.yahoo.com> Message-ID: <200908180114138654065@foxmail.com> i have a painful experience in have my rat FFPE tissue stained with CD31. i failed 15 times and tried combination of 20+ antigen retrieval and primary antibody incubation methods; finally i gave up. now, i am pretty satisfied with the CD31 staining on shock frozen tissue without PFA fixation 2009-08-18 TF ???? sotiris lakis ????? 2009-08-16 17:57:03 ???? histonet ??? ??? [Histonet] CD31(PECAM-1) by novocastra on FFPE rabbit tissue Hi, I have question about CD31(PECAM-1) by novocastra. Has anybody worked with it on FFPE rabbit tissue? I know for instance that DAKO's works, but could find no references for the one by novocastra. Moreover I am using a Bond protocol that comes with an antimouse/antirabbit IgG 2nd Ab. I could also use some suggestions about a good blocking reagent for rabbit. Company tells me I need another detection system but that's not an option...So any protocol manipulation ideas are more than wellcome! Thank you in advance Sotiris Lakis Department of Pathology G.Gennimatas Community Hospital Thessaloniki 54635 Hellas (Greece) ___________________________________________________________ ?????????????? Yahoo!; ?????????? ?? ?????????? ???????? (spam); ?? Yahoo! Mail ???????? ??? ???????? ?????? ????????? ???? ??? ??????????? ????????? http://login.yahoo.com/config/mail?.intl=gr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Mon Aug 17 14:59:09 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Mon Aug 17 15:00:07 2009 Subject: [Histonet] West Nile Message-ID: Has anyone found a good antibody for west Nile FFPE that will work on horses? From Rcartun <@t> harthosp.org Mon Aug 17 15:06:00 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Aug 17 15:06:06 2009 Subject: [Histonet] PCR for Mycobacteria Message-ID: <4A897FE8.7400.0077.1@harthosp.org> Is anyone doing PCR testing for mycobacteria on formalin-fixed, paraffin-embedded tissue? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From kbradshaw <@t> lcpath.com Mon Aug 17 15:29:48 2009 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Mon Aug 17 15:29:55 2009 Subject: [Histonet] Negative Staining Positive In-Reply-To: <4b6c85510908131635o328b4625pef794bc566d43294@mail.gmail.com> Message-ID: <1b0f6c4818be2f488847eff30207b71a@mail2.lcpath.com> We started having the same problem about 6 months ago. We were advised to add a pre-treatment option of over the counter hydrogen peroxide. For the most part it has taken care of the problem. Kari L. Bradshaw HT(ASCP) Laboratory Manager Lower Columbia Pathologists (360)425-5620 kbradshaw@lcpath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histonet Alias Sent: Thursday, August 13, 2009 4:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative Staining Positive Has anyone noticed their negative mouse having some positive staining using the Ventana Benchmark XT? We use the iview detection. Any ideas? Thank you. -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garret.t.miyamoto <@t> us.army.mil Mon Aug 17 16:41:07 2009 From: garret.t.miyamoto <@t> us.army.mil (Miyamoto, Garret T Mr CIV USA USAMEDCOM) Date: Mon Aug 17 16:41:17 2009 Subject: [Histonet] Re: Shandon Excelsior Tissue Processor In-Reply-To: <0KOF004YEGQLV6H0@mail23.us.army.mil> References: <0KOF004YEGQLV6H0@mail23.us.army.mil> Message-ID: Tasha, We have the Excelsior which we received a few weeks ago. We have not used it yet for patient specimens but will very soon. Our test runs went well except some breast tissue (probably cut too thick). Overall, the pathologist was happy with the results. There are many good features, but one of them was the potential savings of alcohol solutions. It has a built in hydrometer that measures the percentage of alcohol in the containers and it will let you know when to change it. We are also looking into the Tissue Tek VIP. We have the 300 model which we like but it is nearing it's life expectancy. Garret Miyamoto Tripler Army Medical Center ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Saturday, August 15, 2009 7:03 am Subject: Histonet Digest, Vol 69, Issue 15 To: histonet@lists.utsouthwestern.edu > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. streptavidin peroxidase (MKing) > 2. HT opening in Colorado (Matthew Lunetta) > 3. unsubscribe (Woodward, Denise) > 4. Job Opening (Konni Black) > 5. Shandon Excelsior Tissue Processor (Tasha Fraser) > 6. Can DAB block an epitope?? (Amos Brooks) > > > ------------------------------------------------------------------- > --- > > Message: 1 > Date: Fri, 14 Aug 2009 13:16:40 -0400 > From: MKing < > Subject: [Histonet] streptavidin peroxidase > To: histonet@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Sigma E-2886 extravidin peroxidase, 1:1000 in 0.1 M PBS or sodium > acetate on almost every IHC variation (fixation, species, primary) we > do. Supposed to be less nonspecific binding than streptavidin. > > Mike King > UF Pharmacology & Therapeutics > > ------------------------------ > > Message: 27 > Date: Fri, 14 Aug 2009 09:56:56 -0700 > From: "Karen Cai" < > Subject: [Histonet] Need help on Streptavidin-HRP conjugate for IHC > To: < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Hi, > I am using ABC kit for DAB-IHC experiment. But somehow, I don't feel it > is a good choice since the background is kinda high. Is there anybody > having some suggestions? Which kind of steptavidin-HRP are you using for > DAB-IHC? From which company? > > Thank you very much for your kind reply, > > > Best, > Karen > > Karen Cai > Research Scientist > Prosci Incorporated > (858) 513-2638 x 204 > (858) 513-2692 Fax > www.prosci-inc.com > > > > ------------------------------ > > Message: 2 > Date: Fri, 14 Aug 2009 14:02:37 -0600 > From: "Matthew Lunetta" < > Subject: [Histonet] HT opening in Colorado > To: < > Message-ID: < > Content-Type: text/plain; charset=US-ASCII > > Hello Histo-Netters, > > We have an HT job opening here in Longmont Colorado, located north of Denver and just NE of Boulder. Please apply through the website. I have attached the link. All new and experianced HT's please apply. Also please add my name to your application so we can track the responces from histo net. > > > http://luh.jobscience.com/JsrApp/index.cfm?cmd=showPositionDetail&nextEvent=doSearchPositions&cobrandId=9000&masterId=luh001&accountId=9F3D0B3C-E71A-17E7-69B649D49CA1C88F&positionId=511568&urlArgList=c2VhcmNoVHlwZT1xdWljayZzdGFydD0xJmNvdW50PTUwJmRlcHRJZD0mam9iSWQ9JmtleXdvcmQ9&bid=1297 > > If the link does not work the web site is www.luhcares.org > > Thanks > Matt Lunetta > BS, HT (ASCP) > > > > > ------------------------------ > > Message: 3 > Date: Fri, 14 Aug 2009 16:20:34 -0400 > From: "Woodward, Denise" < > Subject: [Histonet] unsubscribe > To: "histonet@lists.utsouthwestern.edu" > < > Message-ID: > < > > Content-Type: text/plain; charset="us-ascii" > > > > > ------------------------------ > > Message: 4 > Date: Fri, 14 Aug 2009 13:27:50 -0700 > From: "Konni Black" < > Subject: [Histonet] Job Opening > To: < > Message-ID: < > Content-Type: text/plain; charset="Windows-1252" > > Hello All, > > There is a new histology lab opening in St. Augustine, Florida. Beautiful area on the Palm Coast. > Great pay and working conditions. Extremely flexible hours. In fact, create your own schedule! > > Contact me for details or send resume to kblack@digestivelth.com Don't miss this ground floor opportunity. > > Konni Black > > ------------------------------ > > Message: 5 > Date: Fri, 14 Aug 2009 14:47:54 -0700 > From: Tasha Fraser < > Subject: [Histonet] Shandon Excelsior Tissue Processor > To: "histonet@lists.utsouthwestern.edu" > < > Message-ID: > < > > Content-Type: text/plain; charset="us-ascii" > > Would love to get some feedback hopefully both good and bad in regards > to the Shandon Excelsior Tissue Processor. Looking to purchase a new > tissue processor and I already have a Tissue-Tek VIP 5. I'm going to > Demo a Shandon Excelsior and probably a Leica ASP300 S. I do love the > Tissue-Tek VIP's, but don't feel the need to demo the VIP6. Any > feedback on any of these processors would be greatly appreciated! > > > > Tasha Fraser, HT (ASCP) > > Olympic Medical Center > > Port Angeles, WA > > > ------------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. > > > ------------------------------ > > Message: 6 > Date: Fri, 14 Aug 2009 18:29:25 -0400 > From: Amos Brooks < > Subject: [Histonet] Can DAB block an epitope?? > To: emerald_lake77@yahoo.com, histonet@lists.utsouthwestern.edu > Message-ID: > < > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Gustave, > I do this frequently, and it works great with some caveats. First it is > best to use a *very* red chromogen (if you choose to use red). I have not > had a lot of luck with AEC the fast red in the DAKO alk phos kit looks great > against DAB. > Colocalization of antigens can be tricky. In this case > immunofluoresfence really is the way to go. Biocare Medical has some great > Fluorescent secondaries. Colocalized chromogens just look muddy and not very > good. The DAB doesn't really 'block' the epitope, but ut does make it a heck > of a lot harder to see. > > Good Luck, > Amos > > > Message: 19 > Date: Fri, 14 Aug 2009 08:37:45 -0700 (PDT) > From: GT Hebert < > Subject: [Histonet] Can DAB block an epitope?? > To: Histonet@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset=iso-8859-1 > > Hello all, > > I was hoping to run a chromogenic immuno to check if two proteins > colocalize. > > Since both antibodies are made in rabbit, I was going to stain one and > visualize with DAB and then heat retrieve (pressure or steam) to drop off > the first antibody and restain with my second rabbit antibody using a > different color to visualize. > > How likely, if at all will the DAB precipitate block / mask the epitope of > my second antibody if indeed they are in a similar location? > > Any info would be greatly appreciated. > > Thank you! > > Regards. > > Gustave > > Gustave T. Hebert > Research Scientist I > Metabolic Disease Research > Wyeth Research > 200 CambridgePark Drive > Cambridge, MA 02140 > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 69, Issue 15 > **************************************** From JWeems <@t> sjha.org Mon Aug 17 16:53:53 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Aug 17 16:54:00 2009 Subject: [Histonet] PCR for Mycobacteria In-Reply-To: <4A897FE8.7400.0077.1@harthosp.org> References: <4A897FE8.7400.0077.1@harthosp.org> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA574092A@ITSSSXM01V6.one.ads.che.org> We send ours to National Jewish.. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Monday, August 17, 2009 16:06 To: Histonet Subject: [Histonet] PCR for Mycobacteria Is anyone doing PCR testing for mycobacteria on formalin-fixed, paraffin-embedded tissue? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From brian <@t> prometheushealthcare.com Mon Aug 17 19:30:10 2009 From: brian <@t> prometheushealthcare.com (Brian- Prometheus) Date: Mon Aug 17 19:30:17 2009 Subject: [Histonet] New Histology Opening in Paramus, NJ Message-ID: <000401ca1f9b$0b8b4930$22a1db90$@com> New Opening with a lab in Paramus, NJ Day shift 5am to 1:30pm or 6:00a to 2:30pm Stresses quality over quantity Prefers ASCP certification and NY state licensure Someone who can multitask, detail oriented IHC experience a plus Salary will commensurate upon experience Minimum 5 yr exp Lab is growing, consistently acquiring new clients. Been in business about a year We offer generous referral bonuses. Please be sure to view our newest lab openings at http://twitter.com/PrometheusBlog Brian Feldman Principal Prometheus Healthcare Office 301-693-9057 Fax 301-368-2478 brian@prometheushealthcare.com www.prometheushealthcare.com From cjbulmer <@t> sbcglobal.net Tue Aug 18 08:56:43 2009 From: cjbulmer <@t> sbcglobal.net (Cindy Bulmer) Date: Tue Aug 18 08:56:47 2009 Subject: [Histonet] Job Opening Message-ID: <264342.39206.qm@web82304.mail.mud.yahoo.com> Central Texas Pathology Laboratory in Waco, TX has a full time position available for an IHC technician.? Must be HT(ASCP) registered.? Experience in IHC is not required, although, good cutting skills is a must. CTPL offers excellent benefits along with competitive salary.? Contact me for more information. ? Cindy ? Cindy Bulmer HT(ASCP) QIHC IHC Supervisor, CTPL Waco, TX 254-752-9621 ext 447 From relia1 <@t> earthlink.net Tue Aug 18 10:07:54 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Aug 18 10:07:57 2009 Subject: [Histonet] RELIA Histology Job Alert Florida licensed histotech needed in Largo, FL Message-ID: Hi Histonetters! I hope you are having a great day. I wanted to post a new position that I am excited about. I am currently working with a private pathology lab in Largo, FL. They are in need of a Florida licensed histotech for a part time position. If you or anyone you know might be interested in part time work and hearing more about this opportunity please contact me. I can be reached at 866-607-3542 or relia1@earthlink.net. Thanks-Pam I also have fulltime positions in MA, CA and NY and management positions in WA, CA and TX. Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net < mailto:relia1@earthlink.net> << http://home.earthlink.net/~relia1>> www.myspace.com/pamatrelia www.twitter.com/pamatrelia From brandonmreed <@t> gmail.com Tue Aug 18 10:12:15 2009 From: brandonmreed <@t> gmail.com (Brandon Reed) Date: Tue Aug 18 10:12:19 2009 Subject: [Histonet] MC3T3 Staining Help Message-ID: Hello, I am a student in a cell physiology lab looking at a MC3T3 pre-osteoblastic cell line with an inverted microscope (phase contrast, bright field, or fluorescent). I am looking to stain the membrane to quantify a morphological parameter. Do you have any suggestions as to what stain I should use and whether I should fix the cells? I appreciate your help. -- Brandon M. Reed Biomedical Engineering Georgia Institute of Technology 678-986-2171 From Brenda <@t> nsh.org Tue Aug 18 10:59:17 2009 From: Brenda <@t> nsh.org (Brenda Royce) Date: Tue Aug 18 10:59:22 2009 Subject: [Histonet] The NSH 35th Annual Symposium/Convention Hosts its First Management Forum Message-ID: The NSH Program Team for the Annual S/C is proud to offer a full day management forum designed to provide tools for a histology manager or supervisor to deal with their daily challenges - budgeting, hiring, legal pitfalls to avoid. Topics to be covered include: * Change Management- Communication Tips: Discuss how to manage change & gain some helpful communication tips * Budget Analysis Tools: Review practical tools for monitoring your budget throughout the year * HR Laws You Need to Know * Hiring Skills: Learn steps to hire the right person the first time * Case Studies- Handling Lay-offs, Consolidating Labs, Competing with Outsourcing * Communicating What You Need to Administration & Pathologists: Learn the buzz words and key phrases you can use to successfully communicate what you need Don't miss this rare opportunity to learn management tools/tips geared for the histology manager. Click here for complete details. Register Now! Online Download Brochure Looking for continuing education? Visit the NSH website at www.nsh.org From WBENTON <@t> umm.edu Tue Aug 18 12:29:01 2009 From: WBENTON <@t> umm.edu (Walter Benton) Date: Tue Aug 18 12:29:42 2009 Subject: [Histonet] Re: Histonet Digest, Vol 69, Issue 18 In-Reply-To: References: Message-ID: <4A8AAC9C.D886.00F4.3@umm.edu> Richard, Focus Laboratories performs that test. We have sent out several cases for it in the past. Message: 5 Date: Mon, 17 Aug 2009 16:06:00 -0400 From: "Richard Cartun" Subject: [Histonet] PCR for Mycobacteria To: "Histonet" Message-ID: <4A897FE8.7400.0077.1@harthosp.org> Content-Type: text/plain; charset=US-ASCII Is anyone doing PCR testing for mycobacteria on formalin-fixed, paraffin-embedded tissue? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Walter Benton Histology Supervisor University of Maryland Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From relia1 <@t> earthlink.net Tue Aug 18 13:33:04 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Aug 18 13:33:06 2009 Subject: [Histonet] RELIA Special Job Alert for NY Licensed histotechs Message-ID: Hi Histonetters! I just received this brand new client request and wanted to post it right away! I am currently working with a growing private histology lab located in Orange County NY. There are several full time dayshift permanent positions and my client offer an excellent salary and benefits. Job Description and requirements: Prepares microscopic tissue slides for diagnostic purposes: performs tissue stains, histology and cytology procedures in department for diagnosis: receives and executes all orders from Pathologist. My client is looking for professionals with the following qualifications: NYS license and an AAS degree in Biological Physical and Sciences. If you or anyone you know are interested in learning more about the position duties and responsibilities please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia www.twitter.com/pamatrelia From pruegg <@t> ihctech.net Tue Aug 18 13:36:46 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Aug 18 13:37:12 2009 Subject: [Histonet] baskets for the VIP 1000 model tissue processor Message-ID: <24A165D0DAE64A10B907A6F35FF9876D@Patsyoffice> Does anyone know where we can get the baskets for the VIP 1000 model tissue processor with these measurements by me: The reservoir is 102 mm high 150 mm deep 185 mm wide The basket I have in there now is 94 mm high 145 mm deep 172 mm wide Used ones would be welcome, Sakura is charging over $800 for a new one that does not seem to even fit. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From DKnutson <@t> primecare.org Tue Aug 18 14:18:07 2009 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Tue Aug 18 14:18:16 2009 Subject: [Histonet] New Lab Monitoring Message-ID: <4F0B7161A6CD524FAD8017D52E1553400A768AF3@exchangent> Fellow Histonetters, I am interested to hear your process in monitoring a new lab for formaldehyde, xylene, etc.... We are moving our lab into a different area of the hospital. So we will be monitoring for xylene and formaldehyde. Does anyone monitor for alcohols also? If all monitors turn out within acceptable numbers, does the monitor need to be repeated or are we good? Thank you in advance for your help and I look forward to reading with interest on how others have completed this. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org From MSHERWOOD <@t> PARTNERS.ORG Tue Aug 18 14:31:45 2009 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Aug 18 14:31:50 2009 Subject: [Histonet] Re: Fading of stains Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9703E23C4C@PHSXMB30.partners.org> To all: Has anyone encountered fading of their counterstain in immunohistochemistry and also fading of the chromagen? If so, what did you do to solve it? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From sbreeden <@t> nmda.nmsu.edu Tue Aug 18 14:48:14 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Aug 18 14:48:18 2009 Subject: [Histonet] Lab Telephone Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46A5F@nmdamailsvr.nmda.ad.nmsu.edu> I'm having a challenge wrapping my head around this one: my boss wants to know why I need a telephone in my new lab in the new building we're moving into in January. Part of this is cost (what isn't a cost issue these days??) but I am to come up with justification for having a telephone IN the lab. The new phones will be cordless but he wants the phone to be in the hallway for what he deems a safety issue (I am not a BSL3 lab). The new phone system will be individual lines for specific locations within our facility. I'm the only tech and I'll have a 22x22' lab and the adjoining (with separate entrance) storage room/volatile storage and I will be doing specimen cut-in in another area altogether. I know I've left out some salient points so if you need more info AND if you have some logical input, please ANSWER offline to me directly. I can't figure out why I would NOT need a telephone... egad! I was asked how many calls (in/out) I make/receive per month as a basis for the decision. OMG. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From DKBoyd <@t> chs.net Tue Aug 18 14:54:06 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Tue Aug 18 14:51:19 2009 Subject: [Histonet] New Lab Monitoring In-Reply-To: <4F0B7161A6CD524FAD8017D52E1553400A768AF3@exchangent> Message-ID: We monitor for formalin. We do not use xylene. We have an outside company that comes in to perform air quality and exchanges. The techs wear formaldehyde badges for an 8 hr. period. The pathologist are monitored for 15 mins. while they are grossing. The technician that dumps formalin off tissues to be discarded wears a badge for 15 mins as well. We are monitored every year. I believe this is an OSHA requirement. If you use xylene you must be monitored as well. Debbie M. Boyd, HT(ASCP),Chief Histologist, Southside Regional Medical Center, 200 Medical Park Boulevard, Petersburg, Va. 23805, T: 804-765-5050, F: 804-765-5582, dkboyd@chs.net "Knutson, Deanne" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/18/2009 03:21 PM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] New Lab Monitoring Fellow Histonetters, I am interested to hear your process in monitoring a new lab for formaldehyde, xylene, etc.... We are moving our lab into a different area of the hospital. So we will be monitoring for xylene and formaldehyde. Does anyone monitor for alcohols also? If all monitors turn out within acceptable numbers, does the monitor need to be repeated or are we good? Thank you in advance for your help and I look forward to reading with interest on how others have completed this. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Norm.Burnham <@t> propath.com Tue Aug 18 14:54:05 2009 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Tue Aug 18 14:54:27 2009 Subject: [Histonet] New Lab Monitoring In-Reply-To: References: <4F0B7161A6CD524FAD8017D52E1553400A768AF3@exchangent> Message-ID: <82C7248978CB50469FD6BA68EBBEFE6701FCAC50@exchange.propathlab.com> This issue and related requirements are well-described in the relevant CAP checklist questions. ____________________________________ Norm Burnham Director of Laboratory Operations ProPath - The Leader in Pathology Services 8267 Elmbrook Drive, Suite 100 Dallas, TX 75247 214.237.1602 Office 214.237.1802 Fax 214.709.7127 Cell Email: norm.burnham@propath.com To learn more about ProPath, please visit http://www.ProPath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DKBoyd@chs.net Sent: Tuesday, August 18, 2009 2:54 PM To: Knutson, Deanne Cc: 'histonet@lists.utsouthwestern.edu'; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] New Lab Monitoring We monitor for formalin. We do not use xylene. We have an outside company that comes in to perform air quality and exchanges. The techs wear formaldehyde badges for an 8 hr. period. The pathologist are monitored for 15 mins. while they are grossing. The technician that dumps formalin off tissues to be discarded wears a badge for 15 mins as well. We are monitored every year. I believe this is an OSHA requirement. If you use xylene you must be monitored as well. Debbie M. Boyd, HT(ASCP),Chief Histologist, Southside Regional Medical Center, 200 Medical Park Boulevard, Petersburg, Va. 23805, T: 804-765-5050, F: 804-765-5582, dkboyd@chs.net "Knutson, Deanne" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/18/2009 03:21 PM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] New Lab Monitoring Fellow Histonetters, I am interested to hear your process in monitoring a new lab for formaldehyde, xylene, etc.... We are moving our lab into a different area of the hospital. So we will be monitoring for xylene and formaldehyde. Does anyone monitor for alcohols also? If all monitors turn out within acceptable numbers, does the monitor need to be repeated or are we good? Thank you in advance for your help and I look forward to reading with interest on how others have completed this. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Aug 18 14:54:22 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 18 14:56:00 2009 Subject: [Histonet] Lab Telephone In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46A5F@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <20800.88982.qm@web65713.mail.ac4.yahoo.com> Sara: Calculate: time to go to and back from the telephone TIMES an average number of calls/day = HOURS walking to take care of phone calls, and that time substract it from 8 hours/day and calculate the PRODUCTIVITY reduction you (and you boss) are going to have because not having a phone near you. Costs are important, but productivity is even more important. Ren? J. --- On Tue, 8/18/09, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] Lab Telephone To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 18, 2009, 3:48 PM I'm having a challenge wrapping my head around this one: my boss wants to know why I need a telephone in my new lab in the new building we're moving into in January.? Part of this is cost (what isn't a cost issue these days??) but I am to come up with justification for having a telephone IN? the lab. The new phones will be cordless but he wants the phone to be in the hallway for what he deems a safety issue (I am not a BSL3 lab).? The new phone system will be individual lines for specific locations within our facility. I'm the only tech and I'll have a 22x22' lab and the adjoining (with separate entrance) storage room/volatile storage and I will be doing specimen cut-in in another area altogether. I know I've left out some salient points so if you need more info AND if you have some logical input, please ANSWER offline to me directly.? I can't figure out why I would NOT need a telephone... egad!? I was asked how many calls (in/out) I make/receive per month as a basis for the decision.? OMG. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Tue Aug 18 15:10:34 2009 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Aug 18 15:10:40 2009 Subject: [Histonet] Lab Telephone In-Reply-To: <20800.88982.qm@web65713.mail.ac4.yahoo.com> Message-ID: Sara, Maybe you're looking a gift horse in the mouth. There have been many days that I WISHED that I had no phone in my lab because it was a constant distraction in an already hectic day. Once your boss has to hand deliver a few messages/orders to you, he/she will most likely provide a phone for you without the need for a justification. Just a Thought, Glen Dawson IHC Manager Milwaukee, WI From: Breeden, Sara Subject: [Histonet] Lab Telephone To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 18, 2009, 3:48 PM I'm having a challenge wrapping my head around this one: my boss wants to know why I need a telephone in my new lab in the new building we're moving into in January. Part of this is cost (what isn't a cost issue these days??) but I am to come up with justification for having a telephone IN the lab. The new phones will be cordless but he wants the phone to be in the hallway for what he deems a safety issue (I am not a BSL3 lab). The new phone system will be individual lines for specific locations within our facility. I'm the only tech and I'll have a 22x22' lab and the adjoining (with separate entrance) storage room/volatile storage and I will be doing specimen cut-in in another area altogether. I know I've left out some salient points so if you need more info AND if you have some logical input, please ANSWER offline to me directly. I can't figure out why I would NOT need a telephone... egad! I was asked how many calls (in/out) I make/receive per month as a basis for the decision. OMG. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbplab <@t> yahoo.com Tue Aug 18 15:13:42 2009 From: mbplab <@t> yahoo.com (Mary Benoit) Date: Tue Aug 18 15:13:45 2009 Subject: [Histonet] PCR testing for Mycobacterium Message-ID: <184135.58322.qm@web43131.mail.sp1.yahoo.com> Richard, Focus Diagnostics is a Reference Lab that tests for Mycobacterium and Cat Scratch. Focus Diagnostics, Inc.? 5785 Corporate Avenue,?? Cypress, California? 90630 test # 51183??? (800) 445-4032 ? Mary F Benoit The Pathology Laboratory 830 Bayou Pines Drive West Lake Charles, La? 70601 From burch007 <@t> mc.duke.edu Tue Aug 18 15:55:47 2009 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Tue Aug 18 15:55:51 2009 Subject: [Histonet] Job Opening in North Carolina Message-ID: The Immunopathology Laboratory at Duke University Medical Center in Durham NC has a technician/technologist position available. Please contact me for further information. Jim Burchette burch007@mc.duke.edu 919.681.3973 From jencres <@t> ca.rr.com Tue Aug 18 19:37:15 2009 From: jencres <@t> ca.rr.com (jennifer cresor mike hough) Date: Tue Aug 18 19:37:16 2009 Subject: [Histonet] Please recommend xylene substitutes Message-ID: <6D5B6774C03444899D9ECB4E1A09592C@jennifercresPC> Hello All, I am looking into switching to a different xylene substitute. I currently use Slide Brite and am having problems with water in it. Please recommend your favorite. Thank you for your response. Jennifer Temecula, CA jencres@ca.rr.com From Marilyn.Tyler <@t> uct.ac.za Wed Aug 19 01:35:05 2009 From: Marilyn.Tyler <@t> uct.ac.za (Marilyn Tyler) Date: Wed Aug 19 01:35:17 2009 Subject: [Histonet] immunohisto c-MYC and APC Message-ID: <4A8BB939020000900006D3AC@gwiasmtp.uct.ac.za> Morning to All Please need some help. Anyone used the following antibodies on FFPE human tissue. Novocastra mouse monoclonal c-MYC(oncoprotein) clone 9E11 and APC (adenomatous polyposis Coli)clone EMM43. Have tried with and without retrieval. Tried different retrievals No staining. Thanks Marilyn From jqb7 <@t> cdc.gov Wed Aug 19 05:05:38 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Aug 19 05:06:00 2009 Subject: [Histonet] Lab Telephone In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46A5F@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46A5F@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE101AD35AF@LTA3VS011.ees.hhs.gov> When our new lab was built they gave us phones that could only be used for dialing out...and none in the smaller, procedure rooms. We argued that the tissue processors and other instruments were often in these procedure rooms and it would be handy if we needed a service tech to walk us through a problem. We ended up with the phones in the procedure rooms and also a phone that allowed incoming calls in the main lab. That way we could call the lab looking for individuals without having to run around looking for them. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, August 18, 2009 3:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lab Telephone I'm having a challenge wrapping my head around this one: my boss wants to know why I need a telephone in my new lab in the new building we're moving into in January. Part of this is cost (what isn't a cost issue these days??) but I am to come up with justification for having a telephone IN the lab. The new phones will be cordless but he wants the phone to be in the hallway for what he deems a safety issue (I am not a BSL3 lab). The new phone system will be individual lines for specific locations within our facility. I'm the only tech and I'll have a 22x22' lab and the adjoining (with separate entrance) storage room/volatile storage and I will be doing specimen cut-in in another area altogether. I know I've left out some salient points so if you need more info AND if you have some logical input, please ANSWER offline to me directly. I can't figure out why I would NOT need a telephone... egad! I was asked how many calls (in/out) I make/receive per month as a basis for the decision. OMG. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From philip_manfre <@t> merck.com Wed Aug 19 06:24:05 2009 From: philip_manfre <@t> merck.com (Manfre, Philip) Date: Wed Aug 19 06:24:11 2009 Subject: [Histonet] Please recommend xylene substitutes In-Reply-To: <6D5B6774C03444899D9ECB4E1A09592C@jennifercresPC> References: <6D5B6774C03444899D9ECB4E1A09592C@jennifercresPC> Message-ID: Jennifer, Have you tried Histoclear? It is great for deparaffinizing - we still use xylene at the end of dehydration of slides but it might be okay for that too. We use xylene for processing. Phil. Philip Manfre, BA, HT(ASCP) Senior Research Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jennifer cresor mike hough Sent: Tuesday, August 18, 2009 8:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Please recommend xylene substitutes Hello All, I am looking into switching to a different xylene substitute. I currently use Slide Brite and am having problems with water in it. Please recommend your favorite. Thank you for your response. Jennifer Temecula, CA jencres@ca.rr.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From sbreeden <@t> nmda.nmsu.edu Wed Aug 19 07:14:11 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Aug 19 07:14:16 2009 Subject: [Histonet] Da Phone Issue Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46A74@nmdamailsvr.nmda.ad.nmsu.edu> Thank you to everyone that replied to my (ridiculous) phone justification message! I have many very good, logical, sensible, common-sense reasons there should be a phone IN the lab and I will use every one of them in a short, bullet-point message to my boss. Another reason Histonet is such a priceless device - albeit usually used for more worthy reasons than justifying having a telephone in a histo lab! Let me see... how long until I can retire? WOA - 18 months! Anybody need a p.r.n. in New England??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From lblazek <@t> digestivespecialists.com Wed Aug 19 07:25:33 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Aug 19 07:22:30 2009 Subject: [Histonet] RE: Da Phone Issue In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B02E46A74@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B02E46A74@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E390898A718E8@IBMB7Exchange.digestivespecialists.com> Is Ohio close enough? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, August 19, 2009 8:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Da Phone Issue Thank you to everyone that replied to my (ridiculous) phone justification message! I have many very good, logical, sensible, common-sense reasons there should be a phone IN the lab and I will use every one of them in a short, bullet-point message to my boss. Another reason Histonet is such a priceless device - albeit usually used for more worthy reasons than justifying having a telephone in a histo lab! Let me see... how long until I can retire? WOA - 18 months! Anybody need a p.r.n. in New England??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rick.Garnhart <@t> memorialhealthsystem.com Wed Aug 19 10:34:58 2009 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Wed Aug 19 10:35:03 2009 Subject: [Histonet] Tech vs Professional In-Reply-To: Message-ID: Does anyone out in histology have any documentation from CMS, ASCP, or NSH that breaks down the job duties or areas for what is covered under Tech and professional part of the CPT billing codes. For example is Grossing part of the a profee or tech component. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. From brett_connolly <@t> merck.com Wed Aug 19 11:18:13 2009 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Aug 19 11:18:16 2009 Subject: [Histonet] Evan's Blue - blood brain barrier Message-ID: <63EA0607835FBA4689CEA9EA8B4826920238C3C3@usctmx1141.merck.com> We will be infusing Evan's Blue to assess blood brain barrier disruption in some rats brains. The current plan is to follow this with saline perfusion to clear the vasculature and then perfuse with fixative. Brains will then be sunk in sucrose and frozen for sectioning, but I am wondering if anyone knows if Evan's Blue withstands processing to paraffin? Thanks for any tips/ info, etc. Brett Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From talulahgosh <@t> gmail.com Wed Aug 19 11:49:12 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Aug 19 11:49:20 2009 Subject: [Histonet] in situ with paraffin sections Message-ID: Hello I've been reading a few protocols for RNA in situ hybridization with paraffin sections. One of the suggestions is to dehydrate the sections on the first day after rehydration and pre-hybridization processing (para fix, proteinase K, acetylation, pbs washes between all). This seems counter intuitive to me, or at least unnecessary. Any suggestions why this would be done? Our protocol does not involve this, by the way, and it works fine except for the tissue tearing. Emily "One of the defining characteristics of modern surgery was that patients ought to survive it." --Peter Stanley, For Fear of Pain: British Surgery, 1790-1850 From Sandra.Harrison3 <@t> va.gov Wed Aug 19 12:01:07 2009 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Wed Aug 19 12:02:08 2009 Subject: [Histonet] job opening, Minneapolis VA Message-ID: DEPT. OF VETERAN AFFAIRS MEDICAL CENTER, MINNEPOLIS, MN. Full time Histotech. opportunity at Minneapolis VA. BS or BA in Biology. HT cert. required, HTL preferred. Prefer 5 yr. exp. IHC experience a plus. Effective interpersonal skills required. Holiday, evenings and weekends off. Excellent bene's. Detail oriented. Responsible for technical and procedural operations of the dept., performing quality control, quality improvement and regulatory compliance tasks. Please e-mail resume to Sandra Harrison, Histology Supervisor, at Sandra.Harrison3@va.gov. Principals only. No recruiters please. From bakerj <@t> umich.edu Wed Aug 19 13:07:27 2009 From: bakerj <@t> umich.edu (John Baker) Date: Wed Aug 19 13:07:34 2009 Subject: [Histonet] destaining bone Message-ID: <848838E7-10A4-412C-90D3-9E42D56E4D6D@umich.edu> Hello All, I hope someone can assist us with a protocol for destaining some mouse ulna. A student stained his bones for 12 hours in 1% basic fuchsin in graded ethyl alcohol from 80 % , 90 % and then 100% as a prestain then into 100 % ethyl alcohol to destain some prior to pmma plastic embedding and sectioning to look at microdamage in the cortical bone. The bones are over stained and if possible we would like to destain the bone and continue on without getting more specimens. Any suggestions are welcome. Thank you in advance. John Baker and Mathieu Davis John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 From SAllen <@t> exchange.hsc.mb.ca Wed Aug 19 15:56:40 2009 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Wed Aug 19 15:57:12 2009 Subject: [Histonet] Amyloid staining on frozen muscle bx's Message-ID: Hi, What stain works for amyloid on frozen muscle bx tissue? I did the a Congo Red stain without much success. Thanks Sharon sallen@hsc.mb.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From marianne.kitchell <@t> novusint.com Mon Aug 17 12:25:38 2009 From: marianne.kitchell <@t> novusint.com (Kitchell, Marianne) Date: Wed Aug 19 15:59:53 2009 Subject: [Histonet] anti-bromodeoxyuridine staining Message-ID: <1D77A16CB542A1439E18AD8D6750957D67433F@stlmail02.novusint.com> I am having a problem with my anti-bromodeoxyuridine (BRDU) stain. I have done this for many years on chicken tissues fixed in Notox. Now I have rat tissues (Notox again) and sometimes I get everything staining (all nuclei) and sometimes I get next to nothing to stain. I have recently started cutting all my sections (paraffin) at 4 um. Instead of 5um which may be a partial effect. I am pretty sure that my problem is in the 2.0 N Hydrochloric acid (HCl) step and/or the trypsin step. It seems that if I use freshly prepared HCl, I get a better response than if I have previously prepared and stored my 2.0 N HCl or buy 2.0 N HCl prediluted. This in turn affects the amount of time required in the subsequent trypsin step. Every time I get a new bottle of HCl I need to retitrate the whole stain. Any help here so I am not redoing this into infinity? I am starting to live the movie Groundhog Day. I have never been able to perform this stain without an additional trypsin step even though I have seen it done this way in the literature. Thank you all in advance. Marianne Kitchell Novus International St. Charles, Mo. CONFIDENTIALITY NOTE The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, please be advised that any reading, review, forwarding, dissemination, distribution or copying, of this communication or any attachment(s) are strictly prohibited. If you have received this e-mail in error, please so notify the sender immediately. Also, please delete it and all attachments from any servers or other hard drives. From S.A.Williams <@t> liverpool.ac.uk Tue Aug 18 06:50:57 2009 From: S.A.Williams <@t> liverpool.ac.uk (Williams, Sean) Date: Wed Aug 19 15:59:55 2009 Subject: [Histonet] bone sections Message-ID: <9C69E01E421B6D4B852CA0B46CB42BFA5CD36A53E1@STAFFMBX1.livad.liv.ac.uk> Hi Everyone Could any one tell me how they keep bone sections adhered to slides when staining. I'Ve tried polysine slides, pva glue slides, different ways of drying the sections and for different lenghts of time but nothing seems to work for every section. I'm only staining them with H&E. Thanks Marie O'Brien (liverpool vet path - histology) From marianne.kitchell <@t> novusint.com Wed Aug 19 08:25:09 2009 From: marianne.kitchell <@t> novusint.com (Kitchell, Marianne) Date: Wed Aug 19 15:59:57 2009 Subject: [Histonet] Please recommend xylene substitutes References: <6D5B6774C03444899D9ECB4E1A09592C@jennifercresPC> Message-ID: <1D77A16CB542A1439E18AD8D6750957D4BF7@stlmail02.novusint.com> I have used Clear Rite 3 for may years. It is not as forgiving as xylene when it comes to water, but if you keep it fresh you will be fine. Marianne Kitchell Novus INternational ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of jennifer cresor mike hough Sent: Tue 8/18/2009 7:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Please recommend xylene substitutes Hello All, I am looking into switching to a different xylene substitute. I currently use Slide Brite and am having problems with water in it. Please recommend your favorite. Thank you for your response. Jennifer Temecula, CA jencres@ca.rr.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTE The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, please be advised that any reading, review, forwarding, dissemination, distribution or copying, of this communication or any attachment(s) are strictly prohibited. If you have received this e-mail in error, please so notify the sender immediately. Also, please delete it and all attachments from any servers or other hard drives. From ratliffjack <@t> hotmail.com Wed Aug 19 16:37:33 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Wed Aug 19 16:37:25 2009 Subject: [Histonet] bone sections In-Reply-To: <9C69E01E421B6D4B852CA0B46CB42BFA5CD36A53E1@STAFFMBX1.livad.liv.ac.uk> References: <9C69E01E421B6D4B852CA0B46CB42BFA5CD36A53E1@STAFFMBX1.livad.liv.ac.uk> Message-ID: Are you working with resin or paraffin sections? Jack Sent from my iPhone On Aug 18, 2009, at 6:50 AM, "Williams, Sean" wrote: > Hi Everyone > > Could any one tell me how they keep bone sections adhered to slides > when staining. I'Ve tried polysine slides, pva glue slides, > different ways of drying the sections and for different lenghts of > time but nothing seems to work for every section. > I'm only staining them with H&E. > > Thanks > Marie O'Brien (liverpool vet path - histology) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AnthonyH <@t> chw.edu.au Wed Aug 19 17:19:04 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Aug 19 17:19:16 2009 Subject: [Histonet] in situ with paraffin sections In-Reply-To: Message-ID: Emily, Unfortunately, there are a lot of superfluous steps involved in ISH. It reminds me of the initial IPX protocols. There were many unfathomable steps included that seemed to incite a feeling of magic required to obtain results. Simplifying the technique was one of the main reasons why routine labs were able to utilise it. I hope the same will occur with ISH. IPX and ISH are similar: Block the endogenous enzyme Reverse the formalin cross-linking Apply the probe or antibody Demonstrate the bound probe or antibody. Stringency washes might be required if there is a high possibility of cross-hybridisation. Tissue sections will react differently to Molecular biology nucleic acid containing tubes and gels Granted, for DNA hybridisations you will need to denature the DNA strand but overall that is about it. I would keep it simple and go from there. Many of the added steps were added by researchers because they seemed like the right thing to do not because they were required. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, 20 August 2009 2:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] in situ with paraffin sections Hello I've been reading a few protocols for RNA in situ hybridization with paraffin sections. One of the suggestions is to dehydrate the sections on the first day after rehydration and pre-hybridization processing (para fix, proteinase K, acetylation, pbs washes between all). This seems counter intuitive to me, or at least unnecessary. Any suggestions why this would be done? Our protocol does not involve this, by the way, and it works fine except for the tissue tearing. Emily "One of the defining characteristics of modern surgery was that patients ought to survive it." --Peter Stanley, For Fear of Pain: British Surgery, 1790-1850 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Wed Aug 19 17:26:47 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Aug 19 17:27:00 2009 Subject: [Histonet] Amyloid staining on frozen muscle bx's In-Reply-To: Message-ID: Sharon, For successful amyloid staining with Congo red, formalin fixation might be required. I have not been able to find any references for this except that formalin is used in the demonstration of amyloid in fat aspirations (reference: Duston et al (1987) Am J Med 82:412-414) Have you tried one of the metachromatic dyes (eg Crystal Violet)? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Allen Sent: Thursday, 20 August 2009 6:57 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Amyloid staining on frozen muscle bx's Hi, What stain works for amyloid on frozen muscle bx tissue? I did the a Congo Red stain without much success. Thanks Sharon sallen@hsc.mb.ca ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From anonwums1 <@t> gmail.com Wed Aug 19 19:02:23 2009 From: anonwums1 <@t> gmail.com (Adam .) Date: Wed Aug 19 19:02:28 2009 Subject: [Histonet] anti-GFP antibody Message-ID: <858249120908191702v16bb793ei50bdf08de06f28dd@mail.gmail.com> Hi all, I am looking for a good anti-GFP antibody. I plan on using it for dual immunofluorescence on mouse bone in paraformaldehyde fixed, paraffin embedded sections, most likely with some antigen retrieval. Here's the main problem: I want to use it with a goat-anti-mouse primary and in a separate assay with a rabbit-anti-mouse antibody, both of which are staining mouse bone. In order to save money and optimization time, I would prefer if it were raised in a species other than goat and rabbit so I could use it for both assays. I also want to avoid mouse antibodies to avoid the mouse-on-mouse issue. Alternatively, I could buy two antibodies (rabbit and goat) and use them in the respective assays. Or is there another way that's non-obvious? Thanks, Adam From annigyg <@t> gmail.com Thu Aug 20 02:40:54 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Aug 20 02:41:12 2009 Subject: [Histonet] Tech vs Professional In-Reply-To: References: Message-ID: get the CPT 2009 manual - it is all explained there 2009/8/19 > > Does anyone out in histology have any documentation from CMS, ASCP, or NSH > that breaks down the job duties or areas for what is covered under Tech and > professional part of the CPT billing codes. For example is Grossing part of > the a profee or tech component. > > > > Rick Garnhart HT(ASCP) > Memorial Health System > Histology Supervisor > 1400 E. Boulder St. > Colorado Springs, CO 80909 > Cell: 719-365-8357 > Ph: 719-365-6926 > Fax: 719-365-6373 > rick.garnhart@memorialhealthsystem.com > > > > Mission: To provide the highest quality health care > Vision: To create an outstanding health system where patients heal and > people thrive > Values: Compassion - Integrity - Quality - Respect - Teamwork > > www.memorialhealthsystem.com > > The information contained in or attached to this electronic message is > privileged and confidential, intended only for the use of the individual(s) > named above. If the reader of this message is not the intended recipient, > or the employee or agent responsible to deliver it to the intended > recipient, you are hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you have received > this communication in error, please inform the sender immediately and > remove any record of this > message._______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From TMcNemar <@t> lmhealth.org Thu Aug 20 05:12:57 2009 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Aug 20 05:13:07 2009 Subject: [Histonet] New Lab Monitoring In-Reply-To: <4F0B7161A6CD524FAD8017D52E1553400A768AF3@exchangent> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E126@lmhsmail.lmhealth.org> I think that OSHA says that you must have 2 acceptable monitoring sessions. After that, you do not have to monitor again unless there is a change in method or environment that may impact ventilation/exposure. We just moved into a new lab last December and have had to monitor about 6 times now before they were all acceptable. We will wait a month or two then monitor again. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Knutson, Deanne Sent: Tuesday, August 18, 2009 3:18 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] New Lab Monitoring Fellow Histonetters, I am interested to hear your process in monitoring a new lab for formaldehyde, xylene, etc.... We are moving our lab into a different area of the hospital. So we will be monitoring for xylene and formaldehyde. Does anyone monitor for alcohols also? If all monitors turn out within acceptable numbers, does the monitor need to be repeated or are we good? Thank you in advance for your help and I look forward to reading with interest on how others have completed this. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org From MDiCarlo <@t> KaleidaHealth.Org Thu Aug 20 07:02:34 2009 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Thu Aug 20 07:02:41 2009 Subject: [Histonet] bone sections In-Reply-To: <9C69E01E421B6D4B852CA0B46CB42BFA5CD36A53E1@STAFFMBX1.livad.liv.ac.uk> References: <9C69E01E421B6D4B852CA0B46CB42BFA5CD36A53E1@STAFFMBX1.livad.liv.ac.uk> Message-ID: <1B73766A27A1554CB2729B6432E81301015C7857@KALEXMB04.KaleidaHealth.org> Marie, I make a 5% Titebond solution from Titebond II which can be purchased at any hardware store such as Home Depot, etc. and I rarely lose sections when H&E staining on paraffin embedded bones. Right before sectioning, I take a pipette using the 5% titebond and dab all the corners and center of the slide, take a kimwipe and spread it zigzag across one way all the way down the slide and then turn the slide 1/4 and do it again. Let it air dry a few seconds and then pick up your section. I should mention that I first clean the slides in acid alcohol for 5 minutes, followed by two 95% rinses for 5 minutes each and then dry in a 60 degree oven for 1 hour. Remove from oven, cool, wrap individually with either a kimwipe or paper towel to keep them protected from dust and store in an empty coverslip box. Hope this helps. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Williams, Sean Sent: Tuesday, August 18, 2009 07:51 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] bone sections Hi Everyone Could any one tell me how they keep bone sections adhered to slides when staining. I'Ve tried polysine slides, pva glue slides, different ways of drying the sections and for different lenghts of time but nothing seems to work for every section. I'm only staining them with H&E. Thanks Marie O'Brien (liverpool vet path - histology) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet 2009 Best Places to Work Winner Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From JGarfield <@t> lifecell.com Thu Aug 20 07:17:12 2009 From: JGarfield <@t> lifecell.com (Garfield, Jacqueline) Date: Thu Aug 20 07:17:19 2009 Subject: [Histonet] bone sections In-Reply-To: <1B73766A27A1554CB2729B6432E81301015C7857@KALEXMB04.KaleidaHealth.org> References: <9C69E01E421B6D4B852CA0B46CB42BFA5CD36A53E1@STAFFMBX1.livad.liv.ac.uk> <1B73766A27A1554CB2729B6432E81301015C7857@KALEXMB04.KaleidaHealth.org> Message-ID: Peggy, Have you used this method of adhesion for special stains and IHC staining as well? If so, have you had any interference or background staining? Thank you, Jackie Jacqueline D. Garfield | Manager, Histology Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1182 Email jgarfield@ lifecell.com www.lifcell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DiCarlo, Margaret Sent: Thursday, August 20, 2009 8:03 AM To: Williams, Sean; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] bone sections Marie, I make a 5% Titebond solution from Titebond II which can be purchased at any hardware store such as Home Depot, etc. and I rarely lose sections when H&E staining on paraffin embedded bones. Right before sectioning, I take a pipette using the 5% titebond and dab all the corners and center of the slide, take a kimwipe and spread it zigzag across one way all the way down the slide and then turn the slide 1/4 and do it again. Let it air dry a few seconds and then pick up your section. I should mention that I first clean the slides in acid alcohol for 5 minutes, followed by two 95% rinses for 5 minutes each and then dry in a 60 degree oven for 1 hour. Remove from oven, cool, wrap individually with either a kimwipe or paper towel to keep them protected from dust and store in an empty coverslip box. Hope this helps. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Williams, Sean Sent: Tuesday, August 18, 2009 07:51 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] bone sections Hi Everyone Could any one tell me how they keep bone sections adhered to slides when staining. I'Ve tried polysine slides, pva glue slides, different ways of drying the sections and for different lenghts of time but nothing seems to work for every section. I'm only staining them with H&E. Thanks Marie O'Brien (liverpool vet path - histology) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet 2009 Best Places to Work Winner Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MDiCarlo <@t> KaleidaHealth.Org Thu Aug 20 07:58:47 2009 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Thu Aug 20 07:58:54 2009 Subject: [Histonet] bone sections In-Reply-To: References: <9C69E01E421B6D4B852CA0B46CB42BFA5CD36A53E1@STAFFMBX1.livad.liv.ac.uk> <1B73766A27A1554CB2729B6432E81301015C7857@KALEXMB04.KaleidaHealth.org> Message-ID: <1B73766A27A1554CB2729B6432E81301015C7859@KALEXMB04.KaleidaHealth.org> Jackie, Personally, I have not used this method of adhesion for special stains and IHC staining because I only stain for H&E. However, I know the Pathology department here has used it in the past for both special stains and IHC and has not had any interference or background staining. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 -----Original Message----- From: Garfield, Jacqueline [mailto:JGarfield@lifecell.com] Sent: Thursday, August 20, 2009 08:17 To: DiCarlo, Margaret Cc: Williams, Sean; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] bone sections Peggy, Have you used this method of adhesion for special stains and IHC staining as well? If so, have you had any interference or background staining? Thank you, Jackie Jacqueline D. Garfield | Manager, Histology Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1182 Email jgarfield@ lifecell.com www.lifcell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DiCarlo, Margaret Sent: Thursday, August 20, 2009 8:03 AM To: Williams, Sean; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] bone sections Marie, I make a 5% Titebond solution from Titebond II which can be purchased at any hardware store such as Home Depot, etc. and I rarely lose sections when H&E staining on paraffin embedded bones. Right before sectioning, I take a pipette using the 5% titebond and dab all the corners and center of the slide, take a kimwipe and spread it zigzag across one way all the way down the slide and then turn the slide 1/4 and do it again. Let it air dry a few seconds and then pick up your section. I should mention that I first clean the slides in acid alcohol for 5 minutes, followed by two 95% rinses for 5 minutes each and then dry in a 60 degree oven for 1 hour. Remove from oven, cool, wrap individually with either a kimwipe or paper towel to keep them protected from dust and store in an empty coverslip box. Hope this helps. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Williams, Sean Sent: Tuesday, August 18, 2009 07:51 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] bone sections Hi Everyone Could any one tell me how they keep bone sections adhered to slides when staining. I'Ve tried polysine slides, pva glue slides, different ways of drying the sections and for different lenghts of time but nothing seems to work for every section. I'm only staining them with H&E. Thanks Marie O'Brien (liverpool vet path - histology) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet 2009 Best Places to Work Winner Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet 2009 Best Places to Work Winner Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From Edina.Paal <@t> va.gov Thu Aug 20 08:07:02 2009 From: Edina.Paal <@t> va.gov (Paal, Edina E.) Date: Thu Aug 20 08:07:06 2009 Subject: [Histonet] PTHrP IHC Message-ID: <7193324353F58A41A2B8BCD0957387F904777681@VHAV05MSGA1.v05.med.va.gov> Hi Everyone, Could you, please help me finding a reference lab that would offer PTHrP immunohistochemistry? I know, the antibody is commercially available so somebody must do it, but who??? Thanks for the help Edina Paal, M.D. Pathologist, Cytopathologist, Director of Blood Bank Pathology and Laboratory Medicine Service VA Medical Center, Washington DC 50 Irving St, N.W., Rm GB205 Washington, DC 20422 Tel: (202) 518-4619 Fax: (202) 745-8284 From akemiat3377 <@t> yahoo.com Thu Aug 20 09:57:33 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Aug 20 09:57:35 2009 Subject: [Histonet] Reimbursement for IHC Tests from Medicare/Medicaid Message-ID: <248989.3041.qm@web31302.mail.mud.yahoo.com> Good Morning Histology Managers, The Chief Medical Director here at my facility proposed a question the other day regarding reimbursement for IHC testing from Medicare /Medicaid patients.? I asked our billing manager, and she was going to investigate the matter, but I know my fellow histology managers out there in histoland are pretty up on these matters, so I am asking once again for your expert knowledge. The laws have been going back and forth regarding how much we can charge, and I have been out of the loop for awhile.? Can we charge for more than (2) IHC tests for these patients?? Let's say you want to do a panel that consists of (5) IHC tests, can we only get reimbursed for (2) of those tests?? Any assistance would be greatly appreciated. Thank you in advance for your information, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com From micropathlabs <@t> yahoo.com Thu Aug 20 09:59:59 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Thu Aug 20 10:00:03 2009 Subject: [Histonet] Disinfectant Message-ID: <742468.86625.qm@web57802.mail.re3.yahoo.com> Could anyone please?assist me in locating?specific?references for disinfecting work surfaces in?the laboratory? I'm looking for something?from the CDC or OSHA regarding this issue. Thank you in advance! ? Sheila Haas Laboratory Supervisor Micro Path Laboratories From jqb7 <@t> cdc.gov Thu Aug 20 10:06:54 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Aug 20 10:13:40 2009 Subject: [Histonet] Disinfectant In-Reply-To: <742468.86625.qm@web57802.mail.re3.yahoo.com> References: <742468.86625.qm@web57802.mail.re3.yahoo.com> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE101AD35E1@LTA3VS011.ees.hhs.gov> Perhaps this will help. http://www.cdc.gov/mmwr/preview/mmwrhtml/rr5404a2.htm Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, August 20, 2009 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disinfectant Could anyone please?assist me in locating?specific?references for disinfecting work surfaces in?the laboratory? I'm looking for something?from the CDC or OSHA regarding this issue. Thank you in advance! ? Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Thu Aug 20 10:21:10 2009 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Aug 20 10:21:20 2009 Subject: [Histonet] Amyloid staining on frozen muscle bx's In-Reply-To: References: Message-ID: The method at the address below should work on cryostat sections. http://stainsfile.info/StainsFile/stain/amyloid/siriusllew.htm Bryan Llewellyn ----- Original Message ----- From: "Tony Henwood" To: "Sharon Allen" ; Sent: Wednesday, August 19, 2009 3:26 PM Subject: RE: [Histonet] Amyloid staining on frozen muscle bx's Sharon, For successful amyloid staining with Congo red, formalin fixation might be required. I have not been able to find any references for this except that formalin is used in the demonstration of amyloid in fat aspirations (reference: Duston et al (1987) Am J Med 82:412-414) Have you tried one of the metachromatic dyes (eg Crystal Violet)? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Allen Sent: Thursday, 20 August 2009 6:57 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Amyloid staining on frozen muscle bx's Hi, What stain works for amyloid on frozen muscle bx tissue? I did the a Congo Red stain without much success. Thanks Sharon sallen@hsc.mb.ca ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Thu Aug 20 10:29:02 2009 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu Aug 20 10:29:05 2009 Subject: [Histonet] AFB control slides Message-ID: <24A4826E8EF0964D86BC5317306F58A52BC283ED98@mmc-mail.ad.mhsil.com> I have struck out with a vendor for AFB control slides. Anybody have a supplier of AFB control slides? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From billodonnell <@t> catholichealth.net Thu Aug 20 10:35:24 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Aug 20 10:35:40 2009 Subject: [Histonet] AFB control slides In-Reply-To: <24A4826E8EF0964D86BC5317306F58A52BC283ED98@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A52BC283ED98@mmc-mail.ad.mhsil.com> Message-ID: We get ours from Sigma-Aldrich reference A2299-25EA I do not know what they cost, but they are good controls. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, August 20, 2009 10:29 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB control slides I have struck out with a vendor for AFB control slides. Anybody have a supplier of AFB control slides? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kenneth.metzger <@t> aruplab.com Thu Aug 20 10:40:57 2009 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Thu Aug 20 10:41:02 2009 Subject: [Histonet] Tampers Message-ID: Hello Everyone, Can anyone give me a vendor and order # for tampers to be used while embedding? I appreciate any info. Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metzger@aruplab.com (801) 583-2787 x 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From JWeems <@t> sjha.org Thu Aug 20 10:41:22 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Aug 20 10:41:22 2009 Subject: [Histonet] Reimbursement for IHC Tests from Medicare/Medicaid In-Reply-To: <248989.3041.qm@web31302.mail.mud.yahoo.com> References: <248989.3041.qm@web31302.mail.mud.yahoo.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA577E2A6@ITSSSXM01V6.one.ads.che.org> You can charge one CPT 88342 for each ab on each specimen, but not multiple blocks of the same antibody on the same specimen. e.g. You receive 3 sentinel nodes - A, B, C, on a melanoma resection and you do HMB45, S100 and Melan A on each specimen, the charge is 88342 x 6. If you have 2 blocks on A, and B, your charge will still be x 6. I hope this makes sense. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, August 20, 2009 10:58 To: histonet Cc: esandoval@apmglab.com; jsong@apmglab.com; jchanmd@mindspring.com Subject: [Histonet] Reimbursement for IHC Tests from Medicare/Medicaid Good Morning Histology Managers, The Chief Medical Director here at my facility proposed a question the other day regarding reimbursement for IHC testing from Medicare /Medicaid patients. I asked our billing manager, and she was going to investigate the matter, but I know my fellow histology managers out there in histoland are pretty up on these matters, so I am asking once again for your expert knowledge. The laws have been going back and forth regarding how much we can charge, and I have been out of the loop for awhile. Can we charge for more than (2) IHC tests for these patients? Let's say you want to do a panel that consists of (5) IHC tests, can we only get reimbursed for (2) of those tests? Any assistance would be greatly appreciated. Thank you in advance for your information, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From JWeems <@t> sjha.org Thu Aug 20 10:45:46 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Aug 20 10:45:44 2009 Subject: [Histonet] Tampers In-Reply-To: References: Message-ID: <5D64396A0D4A5346BEBC759022AAEAA577E2AC@ITSSSXM01V6.one.ads.che.org> >From Sakura... Tamper, Large (3/cs) 1551 Tamper, Small (3/cs) 1552 http://www.sakura-americas.com/about/contact.lasso Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Thursday, August 20, 2009 11:41 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tampers Hello Everyone, Can anyone give me a vendor and order # for tampers to be used while embedding? I appreciate any info. Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metzger@aruplab.com (801) 583-2787 x 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From NKlemme <@t> sakuraus.com Thu Aug 20 10:51:29 2009 From: NKlemme <@t> sakuraus.com (Nancy Klemme) Date: Thu Aug 20 10:53:06 2009 Subject: [Histonet] RE: Tampers In-Reply-To: References: Message-ID: <782E3A02C2EB2347BEA6DEA69DC7AB865B4D5713CF@sfamail.SAKURAUS.LOCAL> Hello, Ken: The following is from the Sakura on-line electronic catalog. They're available through Cardinal Health or VWR. Be sure to identify the item number as being a Sakura product number. Item No. Description Quantity Price 1551 TAMPER, LARGE THREE $19.00 1552 TAMPER, SMALL THREE $15.00 Kind regards, Nancy Klemme, EduSvcDir - Sakura Finetek USA, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Thursday, August 20, 2009 8:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tampers Hello Everyone, Can anyone give me a vendor and order # for tampers to be used while embedding? I appreciate any info. Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metzger@aruplab.com (801) 583-2787 x 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo20 <@t> hotmail.com Thu Aug 20 12:11:37 2009 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Thu Aug 20 12:13:16 2009 Subject: [Histonet] Immuno question Message-ID: Does anyone use anti-trypsin (not alpha-1-anti-trypsin) for Acinar carcinoma on paraffin IHC? Thank you so much! Paula Wilder _________________________________________________________________ With Windows Live, you can organize, edit, and share your photos. http://www.windowslive.com/Desktop/PhotoGallery From tifei <@t> foxmail.com Thu Aug 20 12:45:11 2009 From: tifei <@t> foxmail.com (TF) Date: Thu Aug 20 12:45:33 2009 Subject: [Histonet] anti-bromodeoxyuridine staining References: <1D77A16CB542A1439E18AD8D6750957D67433F@stlmail02.novusint.com> Message-ID: <200908210145051737127@foxmail.com> dXNlIDROIEhDbCwgbm8gdHJ5cHNpbiEgMjAgbWluIHJvb210ZW1wZXJhdHVyZSwgd29ya3MgdmVy eSB3ZWVlZWVlZWVlZWxsbGxsbGxsbGxsbGxsbGwNCg0KDQoyMDA5LTA4LTIxIA0KDQoNCg0KVEYg DQoNCg0KDQq3orz+yMujuiBLaXRjaGVsbCwgTWFyaWFubmUgDQq3osvNyrG85KO6IDIwMDktMDgt MjAgIDA1OjA0OjAxIA0KytW8/sjLo7ogaGlzdG9uZXRAbGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1 IA0Ks63LzaO6IA0K1vfM4qO6IFtIaXN0b25ldF0gYW50aS1icm9tb2Rlb3h5dXJpZGluZSBzdGFp bmluZyANCiANCkkgYW0gaGF2aW5nIGEgcHJvYmxlbSB3aXRoIG15IGFudGktYnJvbW9kZW94eXVy aWRpbmUgKEJSRFUpIHN0YWluLiBJDQpoYXZlIGRvbmUgdGhpcyBmb3IgbWFueSB5ZWFycyBvbiBj aGlja2VuIHRpc3N1ZXMgZml4ZWQgaW4gTm90b3guIA0KTm93IEkgaGF2ZSByYXQgdGlzc3VlcyAo Tm90b3ggYWdhaW4pIGFuZCBzb21ldGltZXMgSSBnZXQgZXZlcnl0aGluZw0Kc3RhaW5pbmcgKGFs bCBudWNsZWkpIGFuZCBzb21ldGltZXMgSSBnZXQgbmV4dCB0byBub3RoaW5nIHRvIHN0YWluLiBJ DQpoYXZlIHJlY2VudGx5IHN0YXJ0ZWQgY3V0dGluZyBhbGwgbXkgc2VjdGlvbnMgKHBhcmFmZmlu KSBhdCA0IHVtLg0KSW5zdGVhZCBvZiA1dW0gd2hpY2ggbWF5IGJlIGEgcGFydGlhbCBlZmZlY3Qu IA0KSSBhbSBwcmV0dHkgc3VyZSB0aGF0IG15IHByb2JsZW0gaXMgaW4gdGhlIDIuMCBOIEh5ZHJv Y2hsb3JpYyBhY2lkIChIQ2wpDQpzdGVwIGFuZC9vciB0aGUgdHJ5cHNpbiBzdGVwLiANCkl0IHNl ZW1zIHRoYXQgaWYgSSB1c2UgZnJlc2hseSBwcmVwYXJlZCBIQ2wsIEkgZ2V0IGEgYmV0dGVyIHJl c3BvbnNlDQp0aGFuIGlmIEkgaGF2ZSBwcmV2aW91c2x5IHByZXBhcmVkIGFuZCBzdG9yZWQgbXkg Mi4wIE4gSENsIG9yIGJ1eSAyLjAgTg0KSENsIHByZWRpbHV0ZWQuIFRoaXMgaW4gdHVybiBhZmZl Y3RzIHRoZSBhbW91bnQgb2YgdGltZSByZXF1aXJlZCBpbiB0aGUNCnN1YnNlcXVlbnQgdHJ5cHNp biBzdGVwLiBFdmVyeSB0aW1lIEkgZ2V0IGEgbmV3IGJvdHRsZSBvZiBIQ2wgSSBuZWVkIHRvDQpy ZXRpdHJhdGUgdGhlIHdob2xlIHN0YWluLiBBbnkgaGVscCBoZXJlIHNvIEkgYW0gbm90IHJlZG9p bmcgdGhpcyBpbnRvDQppbmZpbml0eT8gSSBhbSBzdGFydGluZyB0byBsaXZlIHRoZSBtb3ZpZSBH cm91bmRob2cgRGF5Lg0KSSBoYXZlIG5ldmVyIGJlZW4gYWJsZSB0byBwZXJmb3JtIHRoaXMgc3Rh aW4gd2l0aG91dCBhbiBhZGRpdGlvbmFsDQp0cnlwc2luIHN0ZXAgZXZlbiB0aG91Z2ggSSBoYXZl IHNlZW4gaXQgZG9uZSB0aGlzIHdheSBpbiB0aGUgbGl0ZXJhdHVyZS4NClRoYW5rIHlvdSBhbGwg aW4gYWR2YW5jZS4NCg0KDQpNYXJpYW5uZSBLaXRjaGVsbA0KTm92dXMgSW50ZXJuYXRpb25hbA0K U3QuIENoYXJsZXMsIE1vLg0KDQpDT05GSURFTlRJQUxJVFkgTk9URQ0KVGhlIGluZm9ybWF0aW9u IGNvbnRhaW5lZCBpbiB0aGlzIG1lc3NhZ2UgbWF5IGJlIHByaXZpbGVnZWQgYW5kL29yIGNvbmZp ZGVudGlhbA0KYW5kIHByb3RlY3RlZCBmcm9tIGRpc2Nsb3N1cmUuICBJZiB0aGUgcmVhZGVyIG9m IHRoaXMgbWVzc2FnZSBpcyBub3QgdGhlDQppbnRlbmRlZCByZWNpcGllbnQsIG9yIGFuIGVtcGxv eWVlIG9yIGFnZW50IHJlc3BvbnNpYmxlIGZvciBkZWxpdmVyaW5nIHRoaXMNCm1lc3NhZ2UgdG8g dGhlIGludGVuZGVkIHJlY2lwaWVudCwgcGxlYXNlIGJlIGFkdmlzZWQgdGhhdCBhbnkgcmVhZGlu ZywgcmV2aWV3LA0KZm9yd2FyZGluZywgZGlzc2VtaW5hdGlvbiwgZGlzdHJpYnV0aW9uIG9yIGNv cHlpbmcsIG9mIHRoaXMgY29tbXVuaWNhdGlvbiBvcg0KYW55IGF0dGFjaG1lbnQocykgYXJlIHN0 cmljdGx5IHByb2hpYml0ZWQuICBJZiB5b3UgaGF2ZSByZWNlaXZlZCB0aGlzIGUtbWFpbCBpbg0K ZXJyb3IsIHBsZWFzZSBzbyBub3RpZnkgdGhlIHNlbmRlciBpbW1lZGlhdGVseS4gIEFsc28sIHBs ZWFzZSBkZWxldGUgaXQgYW5kIGFsbA0KYXR0YWNobWVudHMgZnJvbSBhbnkgc2VydmVycyBvciBv dGhlciBoYXJkIGRyaXZlcy4NCiAgICAgICAgICANCl9fX19fX19fX19fX19fX19fX19fX19fX19f X19fX19fX19fX19fX19fX19fX19fDQpIaXN0b25ldCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxp c3RzLnV0c291dGh3ZXN0ZXJuLmVkdQ0KaHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9t YWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0DQo= From JWeems <@t> sjha.org Thu Aug 20 13:35:35 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Aug 20 13:35:39 2009 Subject: [Histonet] Reimbursement for IHC Tests from Medicare/Medicaid In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA577E2A6@ITSSSXM01V6.one.ads.che.org> References: <248989.3041.qm@web31302.mail.mud.yahoo.com> <5D64396A0D4A5346BEBC759022AAEAA577E2A6@ITSSSXM01V6.one.ads.che.org> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA577E348@ITSSSXM01V6.one.ads.che.org> Sorry, Guys.. I can't do basic math... So recounting - it is x 9 - not x 6! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, August 20, 2009 11:41 To: Akemi Allison-Tacha; histonet Cc: jchanmd@mindspring.com; jsong@apmglab.com; esandoval@apmglab.com Subject: RE: [Histonet] Reimbursement for IHC Tests from Medicare/Medicaid You can charge one CPT 88342 for each ab on each specimen, but not multiple blocks of the same antibody on the same specimen. e.g. You receive 3 sentinel nodes - A, B, C, on a melanoma resection and you do HMB45, S100 and Melan A on each specimen, the charge is 88342 x 6. If you have 2 blocks on A, and B, your charge will still be x 6. I hope this makes sense. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, August 20, 2009 10:58 To: histonet Cc: esandoval@apmglab.com; jsong@apmglab.com; jchanmd@mindspring.com Subject: [Histonet] Reimbursement for IHC Tests from Medicare/Medicaid Good Morning Histology Managers, The Chief Medical Director here at my facility proposed a question the other day regarding reimbursement for IHC testing from Medicare /Medicaid patients. I asked our billing manager, and she was going to investigate the matter, but I know my fellow histology managers out there in histoland are pretty up on these matters, so I am asking once again for your expert knowledge. The laws have been going back and forth regarding how much we can charge, and I have been out of the loop for awhile. Can we charge for more than (2) IHC tests for these patients? Let's say you want to do a panel that consists of (5) IHC tests, can we only get reimbursed for (2) of those tests? Any assistance would be greatly appreciated. Thank you in advance for your information, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From carl.hobbs <@t> kcl.ac.uk Thu Aug 20 13:38:36 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Thu Aug 20 13:39:33 2009 Subject: [Histonet] Re: anti-GFP antibody Message-ID: <11D9615B89C10747B1C985966A63D7CA29917429ED@KCL-MAIL04.kclad.ds.kcl.ac.uk> I am sure someone has a cunning combination! I use Abcam's ab13970 chicken anti GFP in Pwax sections after HIER. For me, it is very good Image at Abcam or here : http://www.immunoportal.com/index.php Good luck! carl From thisisann <@t> aol.com Thu Aug 20 14:10:00 2009 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Thu Aug 20 14:10:10 2009 Subject: [Histonet] Grossing Qualifications Message-ID: <8CBEFE0F2DAE554-2A8-3075@webmail-dx05.sysops.aol.com> Can someone tell me where I can get?a listing of the qualifications?required to perform grossing of?small biopsies in NY/NJ (under the direct supervision of a Pathologist). Thank You, Ann From jkiernan <@t> uwo.ca Thu Aug 20 14:47:48 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Aug 20 14:48:06 2009 Subject: [Histonet] destaining bone In-Reply-To: <848838E7-10A4-412C-90D3-9E42D56E4D6D@umich.edu> References: <848838E7-10A4-412C-90D3-9E42D56E4D6D@umich.edu> Message-ID: Acid-alcohol should do the trick if you don't mind decalcifying the bone at the same time. If the basic fuchsine is for showing micro-cracks, decalcification might not be a good idea, so how about domestic bleach (sodium hypochlorite)? John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: John Baker Date: Wednesday, August 19, 2009 14:09 Subject: [Histonet] destaining bone To: Histonet@lists.utsouthwestern.edu > Hello All, I hope someone can assist us with a protocol > for > destaining some mouse ulna. A student stained his bones > for 12 hours > in 1% basic fuchsin in graded ethyl alcohol from 80 % , 90 % and > then > 100% as a prestain then into 100 % ethyl alcohol to destain some > prior > to pmma plastic embedding and sectioning to look at microdamage > in the > cortical bone. The bones are over stained and if possible > we would > like to destain the bone and continue on without getting > more > specimens. Any suggestions are welcome. Thank you in > advance. John > Baker and Mathieu Davis > > John A. Baker > The University of Michigan > Orthopaedic Research Laboratories > Histology Unit > 109 Zina Pitcher Place, 2218 BSRB > Ann Arbor, MI 48109-2200 > 734-936-1635 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Thu Aug 20 15:24:23 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Aug 20 15:24:40 2009 Subject: [Histonet] Evan's Blue - blood brain barrier In-Reply-To: <63EA0607835FBA4689CEA9EA8B4826920238C3C3@usctmx1141.merck.com> References: <63EA0607835FBA4689CEA9EA8B4826920238C3C3@usctmx1141.merck.com> Message-ID: Evans blue (EB) and the closely similar dye trypan blue (TB) bind non-covalently to proteins, notably albumin. The dye-protein complexes are fluorescent. These dyes are OK for gross demonstration of regions with permeable capillaries (neurohypophysis, area postrema etc) They are not well suited to examination of sections, although EB was one of the first tracers of retrograde axonal transport (Kristensson et al 1971 Brain Res. 32:399-406; Kuypers et al 1977 Neurosci. Lett. 6:127-135). In early studies with trypan blue the dye was administered chronically so that some of it ended up inside cells. Quite detailed technical details were given by DF Cappell 1929 (J. Path. Bact. 32:595-708). Clasen et al 1970 (J. Neuropath. Exp. Neurol. 29:266-284) took advantage of EB-albumin fluorescence in a histological study of blood-brain barrier failure, but there are better tracers, depending on the purpose of the investigation. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Connolly, Brett M" Date: Wednesday, August 19, 2009 12:19 Subject: [Histonet] Evan's Blue - blood brain barrier To: histonet@lists.utsouthwestern.edu > We will be infusing Evan's Blue to assess blood brain barrier > disruptionin some rats brains. The current plan is to follow > this with saline > perfusion to clear the vasculature and then perfuse with fixative. > Brains will then be sunk in sucrose and frozen for sectioning, > but I am > wondering if anyone knows if Evan's Blue withstands processing to > paraffin? > > Thanks for any tips/ info, etc. > > Brett > > > Notice: This e-mail message, together with any > attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates (which may be known > outside the United States as Merck Frosst, Merck Sharp & Dohme or > MSD and in Japan, as Banyu - direct contact information for > affiliates is > available at http://www.merck.com/contact/contacts.html) that > may be > confidential, proprietary copyrighted and/or legally privileged. > It is > intended solely for the use of the individual or entity named on this > message. If you are not the intended recipient, and have > received this > message in error, please notify us immediately by reply e-mail and > then delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Thu Aug 20 16:49:04 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Thu Aug 20 16:49:10 2009 Subject: [Histonet] Black Silicone Rubber forfilling Dissecting Dishes? Message-ID: I have some glass petri dishes with a black silicone rubber filling that work great for pinning and dissecting small animal tissues. The rubber works much better than the wax. Problem is, I don't know where the stuff came from, so I can't make any more dissecting plates. Does anyone know where I might buy the liquid for pouring the plates with? Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ With Windows Live, you can organize, edit, and share your photos. http://www.windowslive.com/Desktop/PhotoGallery From tkngflght <@t> yahoo.com Thu Aug 20 17:24:53 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Aug 20 17:24:56 2009 Subject: [Histonet] Tampers---affordable alternate Message-ID: <250565.35519.qm@web50904.mail.re2.yahoo.com> ?Hey Ken from ARUP-- ? We go to the hardware store and pick various sizes of large-headed bolts.? The threads are nice to grip with gloved hands and stay cool as they're taller than the tamper backs.? The heads are thick and hold a nice bit of heat, and with all the different sized we can pick what works for each specimen.? At about $0.15 each--no one freaks out if they are accidentally discarded. ? Hope this helps or at least made you smile.? ? Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing health care professionals, one GREAT fit at?a time. 800.756.3309 From anonwums1 <@t> gmail.com Thu Aug 20 19:19:49 2009 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Aug 20 19:19:54 2009 Subject: [Histonet] Cleaning oil off objectives Message-ID: <858249120908201719p2ab45f1fqa1a31a37575013b5@mail.gmail.com> Hi all, You guys were so helpful on my last question, I'll ask another. We have a microscope shared by the floor with several objectives, and it's pretty common for the non-immersion objectives to get contaminated with oil. I asked the guy who is responsible for the scope about this. He said that they call someone from some company who carefully cleans the objectives with acetone and a Q-tip, which if done right works wonders but if done wrong it can damage the lenses. But he mentioned that the lenses are usually re-contaminated within a few weeks since so many people use the scope, so it's sort of a pointless endeavor. This system seems pretty silly to me... I feel like there must be an easier and cheaper way to clean the lenses without damaging them; I certainly don't want to be responsible for damaging a microscope that costs more than my yearly salary. What do you recommend? Thanks, Adam From Barry.R.Rittman <@t> uth.tmc.edu Thu Aug 20 21:35:51 2009 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Aug 20 21:35:56 2009 Subject: [Histonet] Cleaning oil off objectives In-Reply-To: <858249120908201719p2ab45f1fqa1a31a37575013b5@mail.gmail.com> References: <858249120908201719p2ab45f1fqa1a31a37575013b5@mail.gmail.com> Message-ID: <75A0543E23D3A7458012D9E02EDBEC0004D7A1AA55@UTHCMS1.uthouston.edu> Adam Hi I would strongly recommend that the best approach is to train the people using the microscope, everyone is trainable although for some this may be a long learning curve. The use of a taser with the later individuals is strongly recommended! Several years ago the Zeiss representative in Iowa used the expanded plastic packing beads to wipe off the excess oil as he said this was much more absorbent for oil that lens tissue. We have also seen the use of soft wood tips with oil that is encrusted on, on the understanding that the wood is much softer than the lens.. Never completely happy with that concept. A lot depends on the type of lens that is being used. Some lenses, especially older ones may have a coating that is easily damaged even by Q tips. I would use lens paper first (don't be cheap skate with the lens tissue) then repeat using a small amount of lens cleaner. The most difficult and usually the most contaminated seem to be the 40 due to its working distance. Most of the lens cleaners have isopropyl alcohol and some acetone. If it really does not get all the oil after repeating a couple of times then can use acetone but don't flood the lens just use small amounts and wipe across the face. Follow this with lens cleaner and lens paper. Has always worked for me. This sounds a lengthy procedure but only takes a couple of minutes. Hope that this helps Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adam . [anonwums1@gmail.com] Sent: Thursday, August 20, 2009 7:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cleaning oil off objectives Hi all, You guys were so helpful on my last question, I'll ask another. We have a microscope shared by the floor with several objectives, and it's pretty common for the non-immersion objectives to get contaminated with oil. I asked the guy who is responsible for the scope about this. He said that they call someone from some company who carefully cleans the objectives with acetone and a Q-tip, which if done right works wonders but if done wrong it can damage the lenses. But he mentioned that the lenses are usually re-contaminated within a few weeks since so many people use the scope, so it's sort of a pointless endeavor. This system seems pretty silly to me... I feel like there must be an easier and cheaper way to clean the lenses without damaging them; I certainly don't want to be responsible for damaging a microscope that costs more than my yearly salary. What do you recommend? Thanks, Adam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Thu Aug 20 21:53:58 2009 From: tifei <@t> foxmail.com (TF) Date: Thu Aug 20 21:54:25 2009 Subject: [Histonet] anti-bromodeoxyuridine staining References: <1D77A16CB542A1439E18AD8D6750957D67433F@stlmail02.novusint.com>, <200908210145051737127@foxmail.com>, <4A8D9A8A.1090801@umn.edu> Message-ID: <200908211053529229785@foxmail.com> c3VyZS4NCmJ1dCBpIGd1ZXNzIHRoYXQgd29ya3MgYmVzdCBvbiB0aGluIHNlY3Rpb25zLi4uYnkg dGhlIHdheSwgaSBhbSB1c2luZyA0MCB1bSBicmFpbiBzZWN0aW9ucy4NCg0KDQoNCjIwMDktMDgt MjEgDQoNCg0KDQpURiANCg0KDQoNCreivP7Iy6O6IENvbGxlZW4gRm9yc3RlciANCreiy83Ksbzk o7ogMjAwOS0wOC0yMSAgMDI6NDg6NDcgDQrK1bz+yMujuiB0aWZlaSANCrOty82juiANCtb3zOKj uiBSZTogW0hpc3RvbmV0XSBhbnRpLWJyb21vZGVveHl1cmlkaW5lIHN0YWluaW5nIA0KIA0KVXNp bmcgdGhlIGFudGlnZW4gcmV0cmlldmFsIHdvcmtzIHdlbGwgdG9vLg0KQ29sbGVlbiBGb3JzdGVy 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X19fX19fX19fX19fX19fX19fX18NCj4gSGlzdG9uZXQgbWFpbGluZyBsaXN0DQo+IEhpc3RvbmV0 QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdQ0KPiBodHRwOi8vbGlzdHMudXRzb3V0aHdlc3Rlcm4u ZWR1L21haWxtYW4vbGlzdGluZm8vaGlzdG9uZXQNCj4gICANCj4gLS0tLS0tLS0tLS0tLS0tLS0t LS0tLS0tLS0tLS0tLS0tLS0tLS0tLS0tLS0tLS0tLS0tLS0tLS0tLS0tLS0tLS0tLS0tLS0tDQo+ DQo+IF9fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fDQo+IEhp c3RvbmV0IG1haWxpbmcgbGlzdA0KPiBIaXN0b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUN Cj4gaHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3Rv bmV0DQo+ICAgDQo= From mucram11 <@t> comcast.net Fri Aug 21 07:43:06 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Fri Aug 21 07:43:09 2009 Subject: [Histonet] Cleaning oil off objectives In-Reply-To: <75A0543E23D3A7458012D9E02EDBEC0004D7A1AA55@UTHCMS1.uthouston.edu> References: <858249120908201719p2ab45f1fqa1a31a37575013b5@mail.gmail.com> <75A0543E23D3A7458012D9E02EDBEC0004D7A1AA55@UTHCMS1.uthouston.edu> Message-ID: <000001ca225c$ee91f940$cbb5ebc0$@net> Really like the taser idea! I could have used that for several people over the years. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Thursday, August 20, 2009 10:36 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cleaning oil off objectives Adam Hi I would strongly recommend that the best approach is to train the people using the microscope, everyone is trainable although for some this may be a long learning curve. The use of a taser with the later individuals is strongly recommended! Several years ago the Zeiss representative in Iowa used the expanded plastic packing beads to wipe off the excess oil as he said this was much more absorbent for oil that lens tissue. We have also seen the use of soft wood tips with oil that is encrusted on, on the understanding that the wood is much softer than the lens.. Never completely happy with that concept. A lot depends on the type of lens that is being used. Some lenses, especially older ones may have a coating that is easily damaged even by Q tips. I would use lens paper first (don't be cheap skate with the lens tissue) then repeat using a small amount of lens cleaner. The most difficult and usually the most contaminated seem to be the 40 due to its working distance. Most of the lens cleaners have isopropyl alcohol and some acetone. If it really does not get all the oil after repeating a couple of times then can use acetone but don't flood the lens just use small amounts and wipe across the face. Follow this with lens cleaner and lens paper. Has always worked for me. This sounds a lengthy procedure but only takes a couple of minutes. Hope that this helps Barry ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adam . [anonwums1@gmail.com] Sent: Thursday, August 20, 2009 7:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cleaning oil off objectives Hi all, You guys were so helpful on my last question, I'll ask another. We have a microscope shared by the floor with several objectives, and it's pretty common for the non-immersion objectives to get contaminated with oil. I asked the guy who is responsible for the scope about this. He said that they call someone from some company who carefully cleans the objectives with acetone and a Q-tip, which if done right works wonders but if done wrong it can damage the lenses. But he mentioned that the lenses are usually re-contaminated within a few weeks since so many people use the scope, so it's sort of a pointless endeavor. This system seems pretty silly to me... I feel like there must be an easier and cheaper way to clean the lenses without damaging them; I certainly don't want to be responsible for damaging a microscope that costs more than my yearly salary. What do you recommend? Thanks, Adam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LWatzek <@t> brch.com Fri Aug 21 08:31:46 2009 From: LWatzek <@t> brch.com (Laura Watzek) Date: Fri Aug 21 08:31:53 2009 Subject: [Histonet] Giardia Message-ID: <38F4A4621FCFC249943CC3DFA7FFEEC64B9EB781@Exchange.brch.com> Calling for assistance. Does anyone have a tissue block for Giardia control they are willing to donate, if so contact me off list. I tried NSH but they don't have any available. Thanks everyone and Happy Friday Laura R. Watzek BSCT (ASCP) Pathology Supervisor 800 Meadows Rd. Boca Raton, FL 33486 Phone: (561) 955-4135 Fax: (561) 955-2127 lwatzek@brch.com ----------------------------------------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. From jkiernan <@t> uwo.ca Fri Aug 21 10:10:00 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Aug 21 10:10:10 2009 Subject: [Histonet] Evan's Blue - blood brain barrier In-Reply-To: <63EA0607835FBA4689CEA9EA8B4826920238CA3F@usctmx1141.merck.com> References: <63EA0607835FBA4689CEA9EA8B4826920238C3C3@usctmx1141.merck.com> <63EA0607835FBA4689CEA9EA8B4826920238CA3F@usctmx1141.merck.com> Message-ID: Because most of it it dissolves. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Connolly, Brett M" Date: Friday, August 21, 2009 9:27 Subject: RE: [Histonet] Evan's Blue - blood brain barrier To: John Kiernan > Thanks for the history lesson...so why is EB not well suited for examination on sections?? > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Dept. > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > tel. 215-652-2501 fax. 215-993-6803 > brett_connolly@merck.com > > From: John Kiernan [mailto:jkiernan@uwo.ca] > Sent: Thursday, August 20, 2009 4:24 PM > To: Connolly, Brett M > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Evan's Blue - blood brain barrier > > Evans blue (EB) and the closely similar dye trypan blue (TB) bind non-covalently to proteins, notably albumin. The dye-protein complexes are fluorescent. These dyes are OK for gross demonstration of regions with permeable capillaries (neurohypophysis, area postrema etc) They are not well suited to examination of sections, although EB was one of the first tracers of retrograde axonal transport (Kristensson et al 1971 Brain Res. 32:399-406; Kuypers et al 1977 Neurosci. Lett. 6:127-135). In early studies with trypan blue the dye was administered chronically so that some of it ended up inside cells. Quite detailed technical details were given by DF Cappell 1929 (J. Path. Bact. 32:595-708). Clasen et al 1970 (J. Neuropath. Exp. Neurol. 29:266-284) took advantage of EB-albumin fluorescence in a histological study of blood-brain barrier failure, but there are better tracers, depending on the purpose of the investigation. > > John Kiernan > Anatomy, UWO > London, Canada > = = = > ----- Original Message ----- > From: "Connolly, Brett M" > Date: Wednesday, August 19, 2009 12:19 > Subject: [Histonet] Evan's Blue - blood brain barrier > To: histonet@lists.utsouthwestern.edu > > > We will be infusing Evan's Blue to assess blood brain barrier > > disruptionin some rats brains. The current plan is to follow > > this with saline > > perfusion to clear the vasculature and then perfuse with fixative. > > Brains will then be sunk in sucrose and frozen for sectioning, > > but I am > > wondering if anyone knows if Evan's Blue withstands processing to > > paraffin? > > > > Thanks for any tips/ info, etc. > > > > Brett > > > > > > Notice: This e-mail message, together with any > > attachments, contains > > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > > New Jersey, USA 08889), and/or its affiliates (which may be known > > outside the United States as Merck Frosst, Merck Sharp & Dohme or > > MSD and in Japan, as Banyu - direct contact information for > > affiliates is > > available at http://www.merck.com/contact/contacts.html) that > > may be > > confidential, proprietary copyrighted and/or legally privileged. > > It is > > intended solely for the use of the individual or entity named on this > > message. If you are not the intended recipient, and have > > received this > > message in error, please notify us immediately by reply e-mail and > > then delete it from your system. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates (which may be known > outside the United States as Merck Frosst, Merck Sharp & Dohme or > MSD and in Japan, as Banyu - direct contact information for affiliates is > available at http://www.merck.com/contact/contacts.html) that may be > confidential, proprietary copyrighted and/or legally privileged. It is > intended solely for the use of the individual or entity named on this > message. If you are not the intended recipient, and have received this > message in error, please notify us immediately by reply e-mail and > then delete it from your system. > From cmiller <@t> physlab.com Fri Aug 21 11:09:23 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Aug 21 11:09:29 2009 Subject: [Histonet] refrigerating sliver and gold chloride?? Message-ID: I just noticed that my silver and gold chloride says to store at room temp now. I have always stored them in the refrigerator. Is anyone still doing this?? Old habits die hard. I want to know if this has caused any staining issues for anyone. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From jqb7 <@t> cdc.gov Fri Aug 21 11:12:25 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Aug 21 11:13:05 2009 Subject: [Histonet] refrigerating sliver and gold chloride?? In-Reply-To: References: Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE101AD35FE@LTA3VS011.ees.hhs.gov> I know when commercial Schiff's was no longer required to be kept refrigerated I had my doubts but it worked fine. I am guessing this will be the same. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, August 21, 2009 12:09 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] refrigerating sliver and gold chloride?? I just noticed that my silver and gold chloride says to store at room temp now. I have always stored them in the refrigerator. Is anyone still doing this?? Old habits die hard. I want to know if this has caused any staining issues for anyone. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Fri Aug 21 11:15:50 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Aug 21 11:15:53 2009 Subject: [Histonet] clearing agent for gross specimens In-Reply-To: References: Message-ID: I would appreciate hearing from anyone who routinely utilizes a clearing agent in the gross room to clear fat from tissues to allow lymph nodes to be visualized. I am aware of Dissect Aid but would like to learn of other comparable products. If individuals are using home made solutions are will to share their formula I would appreciate receiving that information as well. Our primary interest is speed of clearing for turnaround time reasons. I am in the midst of a Histosearch search but it is slow going because most discussions refer to tissue processing or staining so it's like looking for a needle in a haystack Thank you all Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 From gu.lang <@t> gmx.at Fri Aug 21 11:20:13 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Aug 21 11:20:21 2009 Subject: AW: [Histonet] refrigerating sliver and gold chloride?? In-Reply-To: References: Message-ID: I think, the commercial solution will be stable at RT until the expiration date - but it will last longer in the refrigerator. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cheri Miller Gesendet: Freitag, 21. August 2009 18:09 An: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Betreff: [Histonet] refrigerating sliver and gold chloride?? I just noticed that my silver and gold chloride says to store at room temp now. I have always stored them in the refrigerator. Is anyone still doing this?? Old habits die hard. I want to know if this has caused any staining issues for anyone. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Fri Aug 21 11:41:41 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Aug 21 11:41:45 2009 Subject: FW: [Histonet] clearing agent for gross specimens Message-ID: Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: Shawn Leslie [mailto:LeslieS@vetmed.ufl.edu] Sent: Friday, August 21, 2009 12:21 PM To: Della Speranza, Vinnie Subject: RE: [Histonet] clearing agent for gross specimens Hi Vinnie, We used to just use Glycerin.......It would clear the fat quite nicely.....then the lymph nodes could be visualized -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Friday, August 21, 2009 12:16 PM To: Histonet Subject: [Histonet] clearing agent for gross specimens I would appreciate hearing from anyone who routinely utilizes a clearing agent in the gross room to clear fat from tissues to allow lymph nodes to be visualized. I am aware of Dissect Aid but would like to learn of other comparable products. If individuals are using home made solutions are will to share their formula I would appreciate receiving that information as well. Our primary interest is speed of clearing for turnaround time reasons. I am in the midst of a Histosearch search but it is slow going because most discussions refer to tissue processing or staining so it's like looking for a needle in a haystack Thank you all Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Fri Aug 21 11:41:55 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Aug 21 11:41:59 2009 Subject: FW: [Histonet] clearing agent for gross specimens Message-ID: Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: Jason McGough [mailto:jmcgough@clinlab.com] Sent: Friday, August 21, 2009 12:25 PM To: Della Speranza, Vinnie Subject: RE: [Histonet] clearing agent for gross specimens We use a home made solution that works very well for lymph node visualization. Here is our formula: 5L ETOH 3.4L distilled water 1.6L 37-40% Formaldehyde 1L Glacial Acetic Acid Let me know if I can be of further assistance. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Della Speranza, Vinnie Sent: Friday, August 21, 2009 10:16 AM To: Histonet Subject: [Histonet] clearing agent for gross specimens I would appreciate hearing from anyone who routinely utilizes a clearing agent in the gross room to clear fat from tissues to allow lymph nodes to be visualized. I am aware of Dissect Aid but would like to learn of other comparable products. If individuals are using home made solutions are will to share their formula I would appreciate receiving that information as well. Our primary interest is speed of clearing for turnaround time reasons. I am in the midst of a Histosearch search but it is slow going because most discussions refer to tissue processing or staining so it's like looking for a needle in a haystack Thank you all Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Fri Aug 21 11:42:46 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Aug 21 11:42:51 2009 Subject: FW: [Histonet] clearing agent for gross specimens Message-ID: Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, August 21, 2009 12:34 PM To: Della Speranza, Vinnie Subject: RE: [Histonet] clearing agent for gross specimens I use just plain acetone. The lymph nodes stand out white in just a few minutes. I have been using for about 6 years with no problems. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Friday, August 21, 2009 11:16 AM To: Histonet Subject: [Histonet] clearing agent for gross specimens I would appreciate hearing from anyone who routinely utilizes a clearing agent in the gross room to clear fat from tissues to allow lymph nodes to be visualized. I am aware of Dissect Aid but would like to learn of other comparable products. If individuals are using home made solutions are will to share their formula I would appreciate receiving that information as well. Our primary interest is speed of clearing for turnaround time reasons. I am in the midst of a Histosearch search but it is slow going because most discussions refer to tissue processing or staining so it's like looking for a needle in a haystack Thank you all Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vapatpxs <@t> yahoo.com Fri Aug 21 11:56:10 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Fri Aug 21 11:56:15 2009 Subject: [Histonet] Cleaning oil off objectives In-Reply-To: <000001ca225c$ee91f940$cbb5ebc0$@net> Message-ID: <263168.48894.qm@web46104.mail.sp1.yahoo.com> I use a dog chewed old stick. On another note, how to clean off dried mounting media. I have several objectives where some goombahs dragged the objectives through wet mounting media. If y'all have any suggestions about removing dried mounting media. Send them my way. 4 of my 5 objectives are no longer objective after having their lenses clouded by the media. ;-) Paula Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Fri, 8/21/09, Pamela Marcum wrote: > From: Pamela Marcum > Subject: RE: [Histonet] Cleaning oil off objectives > To: "'Rittman, Barry R'" , histonet@lists.utsouthwestern.edu > Date: Friday, August 21, 2009, 12:43 PM > Really like the taser idea!? I > could have used that for several people over > the years.? > > Pam Marcum > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Rittman, > Barry R > Sent: Thursday, August 20, 2009 10:36 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Cleaning oil off objectives > > Adam > Hi > I would strongly recommend that the best approach is to > train the people > using the microscope, everyone is trainable although for > some this may be a > long learning curve. > The use of a taser with the later individuals is strongly > recommended! > > Several years ago the Zeiss representative in Iowa used the > expanded plastic > packing beads to wipe off the excess oil as he said this > was much more > absorbent for oil that lens tissue. > We have also seen the use of soft wood tips with oil that > is encrusted on, > on the understanding that the wood is much softer than the > lens.. Never > completely happy with that concept. > A lot depends on the type of lens that is being used. > Some lenses, especially older ones may have a coating that > is easily damaged > even by Q tips. > I would use lens paper first (don't be cheap skate with the > lens tissue) > then repeat using? a small amount of lens cleaner. The > most difficult and > usually the most contaminated seem to be the 40 due to its > working distance. > Most of the lens cleaners have isopropyl alcohol and some > acetone. > If it really does not get all the oil after repeating a > couple of times then > can use acetone but don't flood the lens just use small > amounts and wipe > across the face. Follow this with lens cleaner and lens > paper. > Has always worked for me. This sounds a lengthy procedure > but only takes a > couple of minutes. > Hope that this helps > Barry > > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Adam . > [anonwums1@gmail.com] > Sent: Thursday, August 20, 2009 7:19 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cleaning oil off objectives > > Hi all, > > You guys were so helpful on my last question, I'll ask > another. We have a > microscope shared by the floor with several objectives, and > it's pretty > common for the non-immersion objectives to get contaminated > with oil. I > asked the guy who is responsible for the scope about this. > He said that they > call someone from some company who carefully cleans the > objectives with > acetone and a Q-tip, which if done right works wonders but > if done wrong it > can damage the lenses. But he mentioned that the lenses are > usually > re-contaminated within a few weeks since so many people use > the scope, so > it's sort of a pointless endeavor. This system seems pretty > silly to me... I > feel like there must be an easier and cheaper way to clean > the lenses > without damaging them; I certainly don't want to be > responsible for damaging > a microscope that costs more than my yearly salary. What do > you recommend? > > Thanks, > Adam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Fri Aug 21 12:03:20 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 21 12:03:25 2009 Subject: [Histonet] clearing agent for gross specimens In-Reply-To: Message-ID: <92481.15081.qm@web65708.mail.ac4.yahoo.com> Vinnie: I always used the following solution: ? Absolute ethanol -----------2000 mL Distilled water ---------------- 680 mL 38% ("pure") formalin -------320 mL acetic acid---------------------- 200 mL ? The LN are revealed well Ren? J. ? ? ? --- On Fri, 8/21/09, Della Speranza, Vinnie wrote: From: Della Speranza, Vinnie Subject: [Histonet] clearing agent for gross specimens To: "Histonet" Date: Friday, August 21, 2009, 12:15 PM I would appreciate hearing from anyone who routinely utilizes a clearing agent in the gross room to clear fat from tissues to allow lymph nodes to be visualized. I am aware of Dissect Aid but would like to learn of other comparable products. If individuals are using home made solutions are will to share their formula I would appreciate receiving that information as well. Our primary interest is speed of clearing for turnaround time reasons. I am in the midst of a Histosearch search but it is slow going because most discussions refer to tissue processing or staining so it's like looking for a needle in a haystack Thank you all Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Fri Aug 21 12:21:35 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Aug 21 12:21:39 2009 Subject: [Histonet] FW: clearing agent for gross specimens Message-ID: Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: Cazares, Ruth [mailto:RCazares@schosp.org] Sent: Friday, August 21, 2009 12:58 PM To: Della Speranza, Vinnie Subject: RE: clearing agent for gross specimens Vinnie, We make our own Davidson's fixative, and we also tried Dissect Aid. Comparing the two, our PA and our pathologists liked the homemade Davidsons fixative better than the Dissect Aid. Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Friday, August 21, 2009 11:16 AM To: Histonet Subject: [Histonet] clearing agent for gross specimens I would appreciate hearing from anyone who routinely utilizes a clearing agent in the gross room to clear fat from tissues to allow lymph nodes to be visualized. I am aware of Dissect Aid but would like to learn of other comparable products. If individuals are using home made solutions are will to share their formula I would appreciate receiving that information as well. Our primary interest is speed of clearing for turnaround time reasons. I am in the midst of a Histosearch search but it is slow going because most discussions refer to tissue processing or staining so it's like looking for a needle in a haystack Thank you all Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From rsrichmond <@t> gmail.com Fri Aug 21 12:23:15 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Aug 21 12:23:20 2009 Subject: [Histonet] Re: clearing agent for gross specimens Message-ID: Vinnie Della Speranza in Charleston SC asks: I would appreciate hearing from anyone who routinely utilizes a clearing agent in the gross room to clear fat from tissues to allow lymph nodes to be visualized. I am aware of Dissect Aid but would like to learn of other comparable products. If individuals are using home made solutions are will to share their formula I would appreciate receiving that information as well. Our primary interest is speed of clearing for turnaround time reasons. I am in the midst of a Histosearch search but it is slow going because most discussions refer to tissue processing or staining so it's like looking for a needle in a haystack. ************************************* Most of the proprietary mixes such as Dissect-Aid and O-Fix contain varying amounts of water, alcohol, formaldehyde, and acetic acid. As John Kiernan pointed out some time ago, these formulas aren't very rational. I've had good luck with both Dissect-Aid and O-Fix. When I make my own clearing fixative, I make Davidson's fixative: 3 parts water, 3 parts reagent alcohol, 2 parts 37% formaldehyde, 1 part glacial acetic acid. An obvious point often missed: you cannot post-fix. The tissues must go into the clearing fixative before the neutral buffered formalin the specimen arrives in has time to penetrate, a few hours but not longer. The clearing fixative needs several hours to work, preferably overnight, particularly since such specimens usually arrive late in the day. The fatty lymph node bearing tissue needs to be cut up so that the fixative will penetrate it. Clearing fixatives are most useful with colon resections for cancer. Mesenteric lymph nodes are often small, and very small lymph nodes often contain metastatic colon cancer. These tiny metastases determine treatment: chemotherapy is indicated if lymph nodes are positive. (It's amazing that, with so much riding on it, how little attention is paid to these details.) In my limited experience, clearing fixatives are useful with radical neck dissection specimens. I don't find clearing fixatives necessary with axillary tissue removed in the treatment of breast cancer, and the fixatives may interfere with the immunostains often used with axillary nodes. Most pahtologists I've spoken with about this agree with me. I think there's a good bit of material about this in the Histonet archives - try searching Davidson's fixative. Bob Richmond Samurai Pathologist Knoxville TN From kkwaa <@t> bidmc.harvard.edu Fri Aug 21 12:39:28 2009 From: kkwaa <@t> bidmc.harvard.edu (kkwaa@bidmc.harvard.edu) Date: Fri Aug 21 12:39:41 2009 Subject: [Histonet] C4D Antibody Message-ID: <25A33F7C2B7E2E458182A863929DD9D803490D0A@EVS7.its.caregroup.org> Just wondering if anyone out there routinely works with C4d. I was wondering what you use for retrieval and the Type of detection kit you use. Thanks. Kwadwo Kwaa. BIDMC Pathology Department IHC From gu.lang <@t> gmx.at Fri Aug 21 13:24:54 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Aug 21 13:25:01 2009 Subject: AW: [Histonet] C4D Antibody In-Reply-To: <25A33F7C2B7E2E458182A863929DD9D803490D0A@EVS7.its.caregroup.org> References: <25A33F7C2B7E2E458182A863929DD9D803490D0A@EVS7.its.caregroup.org> Message-ID: We work with the Benchmark XT, ultraview kit (polymer), CC1 retrieval (pH 8-9) 30 min, C4d from Zytomed 1:25 32min, with amplification. Gudrun Lang Akh Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von kkwaa@bidmc.harvard.edu Gesendet: Freitag, 21. August 2009 19:39 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] C4D Antibody Just wondering if anyone out there routinely works with C4d. I was wondering what you use for retrieval and the Type of detection kit you use. Thanks. Kwadwo Kwaa. BIDMC Pathology Department IHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Aug 21 13:36:12 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Aug 21 13:36:19 2009 Subject: AW: [Histonet] Re: clearing agent for gross specimens In-Reply-To: References: Message-ID: <4E8F65CD432545EB973B5DFBACE31686@dielangs.at> A question about the practical grossing of colons with clearing fixative. Do you put the whole colon in the clearing fixative? Or do you cut the mesocolon off and fix it seperated and let the colon itself in NBF? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Robert Richmond Gesendet: Freitag, 21. August 2009 19:23 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: clearing agent for gross specimens Vinnie Della Speranza in Charleston SC asks: I would appreciate hearing from anyone who routinely utilizes a clearing agent in the gross room to clear fat from tissues to allow lymph nodes to be visualized. I am aware of Dissect Aid but would like to learn of other comparable products. If individuals are using home made solutions are will to share their formula I would appreciate receiving that information as well. Our primary interest is speed of clearing for turnaround time reasons. I am in the midst of a Histosearch search but it is slow going because most discussions refer to tissue processing or staining so it's like looking for a needle in a haystack. ************************************* Most of the proprietary mixes such as Dissect-Aid and O-Fix contain varying amounts of water, alcohol, formaldehyde, and acetic acid. As John Kiernan pointed out some time ago, these formulas aren't very rational. I've had good luck with both Dissect-Aid and O-Fix. When I make my own clearing fixative, I make Davidson's fixative: 3 parts water, 3 parts reagent alcohol, 2 parts 37% formaldehyde, 1 part glacial acetic acid. An obvious point often missed: you cannot post-fix. The tissues must go into the clearing fixative before the neutral buffered formalin the specimen arrives in has time to penetrate, a few hours but not longer. The clearing fixative needs several hours to work, preferably overnight, particularly since such specimens usually arrive late in the day. The fatty lymph node bearing tissue needs to be cut up so that the fixative will penetrate it. Clearing fixatives are most useful with colon resections for cancer. Mesenteric lymph nodes are often small, and very small lymph nodes often contain metastatic colon cancer. These tiny metastases determine treatment: chemotherapy is indicated if lymph nodes are positive. (It's amazing that, with so much riding on it, how little attention is paid to these details.) In my limited experience, clearing fixatives are useful with radical neck dissection specimens. I don't find clearing fixatives necessary with axillary tissue removed in the treatment of breast cancer, and the fixatives may interfere with the immunostains often used with axillary nodes. Most pahtologists I've spoken with about this agree with me. I think there's a good bit of material about this in the Histonet archives - try searching Davidson's fixative. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kkwaa <@t> bidmc.harvard.edu Fri Aug 21 14:06:28 2009 From: kkwaa <@t> bidmc.harvard.edu (kkwaa@bidmc.harvard.edu) Date: Fri Aug 21 14:06:46 2009 Subject: [Histonet] C4d Message-ID: <25A33F7C2B7E2E458182A863929DD9D803490D0B@EVS7.its.caregroup.org> Thx Everyone for your input on C4D......I'm running it right now. Trying new things and special thx to Sally Drew. Kwadwo kwaa BIDMC Pathology Department 617-667-5769 From HornHV <@t> archildrens.org Fri Aug 21 14:49:54 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Aug 21 14:49:58 2009 Subject: [Histonet] flex kits Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D833D4@EMAIL.archildrens.org> If any of you are using the flex kits from Dako I would appreciate your feedback on what you think about them good and/or bad. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From dellav <@t> musc.edu Fri Aug 21 15:13:49 2009 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Aug 21 15:13:57 2009 Subject: [Histonet] FW: clearing agent for gross specimens Message-ID: Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: Schneider, Dawn [mailto:Dawn.Schneider@ministryhealth.org] Sent: Friday, August 21, 2009 3:37 PM To: Della Speranza, Vinnie Subject: RE: clearing agent for gross specimens We make our own. Have been using the same one since I before I came here 17 years ago. Here is the recipe: Chloroform (we buy in gallon jug from Fisher) 600 ml. Reagent Alcohol 300 ml Glacial Acetic Acid 100 ml. We usually make up a double batch so we have enough on hand. I have seen the lymph nodes pop out in 30 minutes and have one pathologist who has left the specimen in clearing overnight. It can be used before formalin fixation or after. They use mostly for colon fat that they have trimmed off the colon. We rinse with water for 5 minutes before grossing. Good luck Vinnie. Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 dawn.schneider@ministryhealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Friday, August 21, 2009 12:22 PM To: Histonet Subject: [Histonet] FW: clearing agent for gross specimens Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 -----Original Message----- From: Cazares, Ruth [mailto:RCazares@schosp.org] Sent: Friday, August 21, 2009 12:58 PM To: Della Speranza, Vinnie Subject: RE: clearing agent for gross specimens Vinnie, We make our own Davidson's fixative, and we also tried Dissect Aid. Comparing the two, our PA and our pathologists liked the homemade Davidsons fixative better than the Dissect Aid. Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Friday, August 21, 2009 11:16 AM To: Histonet Subject: [Histonet] clearing agent for gross specimens I would appreciate hearing from anyone who routinely utilizes a clearing agent in the gross room to clear fat from tissues to allow lymph nodes to be visualized. I am aware of Dissect Aid but would like to learn of other comparable products. If individuals are using home made solutions are will to share their formula I would appreciate receiving that information as well. Our primary interest is speed of clearing for turnaround time reasons. I am in the midst of a Histosearch search but it is slow going because most discussions refer to tissue processing or staining so it's like looking for a needle in a haystack Thank you all Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, SC 29425 tel. 843-792-6353 fax. 843-792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From rsrichmond <@t> gmail.com Fri Aug 21 15:57:32 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Aug 21 15:57:36 2009 Subject: [Histonet] Re: clearing agent for gross specimens In-Reply-To: <4E8F65CD432545EB973B5DFBACE31686@dielangs.at> References: <4E8F65CD432545EB973B5DFBACE31686@dielangs.at> Message-ID: Gudrun Lang in Austria asks a question about grossing colons I should have addressed earlier: >>A question about the practical grossing of colons with clearing fixative. Do you put the whole colon in the clearing fixative? Or do you cut the mesocolon off and fix it separated and put the colon itself in NBF?<< You detach the mesenteries, cut them into fairly thin slices, and put the slices in the clearing fixative. The rest of the colon is fixed in neutral buffered formalin, pinned to a board if possible. Both should be fixed overnight. Epiploic appendages and other non-mesenteric fat can be put in the formalin and not further dissected. Bob Richmond Samurai Pathologist Knoxville TN From jkiernan <@t> uwo.ca Fri Aug 21 16:00:28 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Aug 21 16:00:33 2009 Subject: [Histonet] refrigerating sliver and gold chloride?? In-Reply-To: References: Message-ID: There was never any reason to refrigerate "gold chloride" or silver nitrate. These compounds (solid or dissolved) can be kept for many years at room temperature. If the solutions are used repeatedly they eventually deteriorate from contamination with bits of sections, causing a changed appearance. Gold solutions take on a greenish grey hue and flakes of metallic gold eventually settle out. These can easily be recovered and recycled to make "gold chloride" (HAuCl4) again. Clean "gold chloride" solutions keep for ever. I have a few bottles of 0.5% that are still that beautiful yellow colour after about 25 years. Old silver nitrate looks a bit grey, not completely colourless. Re-purifying in a histology lab isn't really feasible. You can precipitate out and collect the silver, but (strangely) refining companies don't want it. Your message mentioned "silver and gold chloride". I don't know a histological use for silver chloride, which is very sensitive to light - goes grey-violet as you look at it. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Cheri Miller Date: Friday, August 21, 2009 12:10 Subject: [Histonet] refrigerating sliver and gold chloride?? To: "histonet@lists.utsouthwestern.edu" Cc: "histonet-bounces@lists.utsouthwestern.edu" > I just noticed that my silver and gold chloride says to store at > room temp now. I have always stored them in the refrigerator. Is > anyone still doing this?? Old habits die hard. I want to know if > this has caused any staining issues for anyone. > > Cheryl Miller HT (ASCP) > Histology Supervisor > Physicians Laboratory,P.C. > Omaha, Ne. > 402 738 5052 > > > ________________________________ > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this > message. If you are not the addressee intended / indicated or > agent responsible for delivering it to the addressee, you are > hereby notified that you are in possession of confidential and > privileged information. Any dissemination, distribution, or > copying of this e-mail is strictly prohibited. If you have > received this message in error, please notify the sender > immediately and delete this email from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From invite+kgaxsgex <@t> facebookmail.com Sat Aug 22 21:10:22 2009 From: invite+kgaxsgex <@t> facebookmail.com (Dianna Uhrich) Date: Sat Aug 22 21:10:28 2009 Subject: [Histonet] Check out my photos on Facebook Message-ID: <44fc5b383d21ce29922ca9fcdab039de@localhost.localdomain> Hi histonet@pathology.swmed.edu, I set up a Facebook profile where I can post my pictures, videos and events and I want to add you as a friend so you can see it. First, you need to join Facebook! Once you join, you can also create your own profile. Thanks, Dianna To sign up for Facebook, follow the link below: http://www.facebook.com/p.php?i=100000081424287&k=Z5LZ26TR45Z1UCF1V1W2YRS&r histonet@pathology.swmed.edu was invited to join Facebook by Dianna Uhrich. If you do not wish to receive this type of email from Facebook in the future, please click on the link below to unsubscribe. http://www.facebook.com/o.php?k=e960e1&u=1207287862&mid=faa23cG47f5c036G0G8 Facebook's offices are located at 1601 S. California Ave., Palo Alto, CA 94304. From rsrichmond <@t> gmail.com Sun Aug 23 12:55:36 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sun Aug 23 12:55:41 2009 Subject: [Histonet] Re: Check out my photos on Facebook Message-ID: Dianna Uhrich notes: >> I set up a Facebook profile where I can post my pictures, videos and events and I want to add you as a friend so you can see it. First, you need to join Facebook! Once you join, you can also create your own profile.<< I'm active on Facebook myself (as Bob Richmond), but usually a graphics server such as Flickr is more appropriate for posting and linking pictures and videos to a list such as Histonet. (I'm bobrichmond on Flickr - there are several other choices such as Google's Picasa, and Photobucket). Bob Richmond Samurai Pathologist Knoxville TN From lhadley <@t> iupui.edu Mon Aug 24 06:48:00 2009 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Mon Aug 24 06:48:37 2009 Subject: [Histonet] RE: flex kits In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D833D4@EMAIL.archildrens.org> References: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D833D4@EMAIL.archildrens.org> Message-ID: <5E6A94F8037F4F49B738F5B6AD1695221402EEE359@iu-mssg-mbx09.ads.iu.edu> Lots of waste! Lee Ann Baldridge IUSM Indpls.,IN ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V [HornHV@archildrens.org] Sent: Friday, August 21, 2009 3:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] flex kits If any of you are using the flex kits from Dako I would appreciate your feedback on what you think about them good and/or bad. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Mon Aug 24 07:58:46 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Mon Aug 24 08:01:48 2009 Subject: [Histonet] Cleaning oil off objectives In-Reply-To: <263168.48894.qm@web46104.mail.sp1.yahoo.com> References: <000001ca225c$ee91f940$cbb5ebc0$@net> <263168.48894.qm@web46104.mail.sp1.yahoo.com> Message-ID: If the contamination is fresh, a bit of spit on a piece of lens paper will usually remove it. Human salivary mucin is an excellent emulsifier. The best thing for removing old contamination is cedarwood oil. Again, use a drop on a piece of lens paper. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello Sent: Friday, August 21, 2009 12:56 PM To: Pamela Marcum; HistoNet Subject: RE: [Histonet] Cleaning oil off objectives I use a dog chewed old stick. On another note, how to clean off dried mounting media. I have several objectives where some goombahs dragged the objectives through wet mounting media. If y'all have any suggestions about removing dried mounting media. Send them my way. 4 of my 5 objectives are no longer objective after having their lenses clouded by the media. ;-) Paula Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Fri, 8/21/09, Pamela Marcum wrote: > From: Pamela Marcum > Subject: RE: [Histonet] Cleaning oil off objectives > To: "'Rittman, Barry R'" , histonet@lists.utsouthwestern.edu > Date: Friday, August 21, 2009, 12:43 PM > Really like the taser idea!? I > could have used that for several people over > the years.? > > Pam Marcum > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Rittman, > Barry R > Sent: Thursday, August 20, 2009 10:36 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Cleaning oil off objectives > > Adam > Hi > I would strongly recommend that the best approach is to > train the people > using the microscope, everyone is trainable although for > some this may be a > long learning curve. > The use of a taser with the later individuals is strongly > recommended! > > Several years ago the Zeiss representative in Iowa used the > expanded plastic > packing beads to wipe off the excess oil as he said this > was much more > absorbent for oil that lens tissue. > We have also seen the use of soft wood tips with oil that > is encrusted on, > on the understanding that the wood is much softer than the > lens.. Never > completely happy with that concept. > A lot depends on the type of lens that is being used. > Some lenses, especially older ones may have a coating that > is easily damaged > even by Q tips. > I would use lens paper first (don't be cheap skate with the > lens tissue) > then repeat using? a small amount of lens cleaner. The > most difficult and > usually the most contaminated seem to be the 40 due to its > working distance. > Most of the lens cleaners have isopropyl alcohol and some > acetone. > If it really does not get all the oil after repeating a > couple of times then > can use acetone but don't flood the lens just use small > amounts and wipe > across the face. Follow this with lens cleaner and lens > paper. > Has always worked for me. This sounds a lengthy procedure > but only takes a > couple of minutes. > Hope that this helps > Barry > > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Adam . > [anonwums1@gmail.com] > Sent: Thursday, August 20, 2009 7:19 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cleaning oil off objectives > > Hi all, > > You guys were so helpful on my last question, I'll ask > another. We have a > microscope shared by the floor with several objectives, and > it's pretty > common for the non-immersion objectives to get contaminated > with oil. I > asked the guy who is responsible for the scope about this. > He said that they > call someone from some company who carefully cleans the > objectives with > acetone and a Q-tip, which if done right works wonders but > if done wrong it > can damage the lenses. But he mentioned that the lenses are > usually > re-contaminated within a few weeks since so many people use > the scope, so > it's sort of a pointless endeavor. This system seems pretty > silly to me... I > feel like there must be an easier and cheaper way to clean > the lenses > without damaging them; I certainly don't want to be > responsible for damaging > a microscope that costs more than my yearly salary. What do > you recommend? > > Thanks, > Adam > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LBUSTAMANTE <@t> cvm.tamu.edu Mon Aug 24 08:28:22 2009 From: LBUSTAMANTE <@t> cvm.tamu.edu (Lin Bustamante) Date: Mon Aug 24 08:28:36 2009 Subject: [Histonet] PCP control Message-ID: <4A924F26.EB3B.00B9.1@cvm.tamu.edu> With the shortage of PCP controls what are you using as a possible alternative? Any information will be greatly appreciated. Thank you. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab. Supervisor Dept. of Veterinary Integrative Biosciences Texas A&M University College Station, TX 77843-4458 (979)845-3177 From mbplab <@t> yahoo.com Mon Aug 24 09:44:22 2009 From: mbplab <@t> yahoo.com (Mary Benoit) Date: Mon Aug 24 09:44:27 2009 Subject: [Histonet] fat clearing reagent Message-ID: <389082.40938.qm@web43145.mail.sp1.yahoo.com> Our home made recipe is :? 1925 mls of 95% alcohol? + 360 mls DH20 + 250 mls of Formaldehyde + 125 mls Glacial Acetic Acid.? Got this 20 years ago from one of my pathologists' journals and it works really well.? Let sit overnight and even the smallest nodes will turn opaque against a somewhat "clear" fatty background. From christina.thurby <@t> bms.com Mon Aug 24 09:58:43 2009 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Mon Aug 24 09:59:18 2009 Subject: [Histonet] reduction/elimination of red cell autofluorescence on FFPE sections for IF examination In-Reply-To: <41f5ad49-c0f7-4bdb-ae7c-8e35d28679ac@ushpwbmsmhp002.one.ads.bms.com> References: <41f5ad49-c0f7-4bdb-ae7c-8e35d28679ac@ushpwbmsmhp002.one.ads.bms.com> Message-ID: Can anyone give feedback on reagents/procedures to use for immunofluorescence to reduce/eliminate red cell autofluorescence on FFPE sections. I am using an indirect method of labeling for two antibodies (FITC and Texas Red). I have not tried 0.1% sodium borohydride. Will this help for formalin fixed specimens? I have read that it is used for gluteraldehyde autofluorescence reduction. If appropriate how long should this reagent be applied to the specimen and at what temperature. This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From christina.thurby <@t> bms.com Mon Aug 24 10:00:50 2009 From: christina.thurby <@t> bms.com (Thurby, Christina) Date: Mon Aug 24 10:01:21 2009 Subject: [Histonet] RE: reduction/elimination of red cell autofluorescence on FFPE sections for IF examination References: <41f5ad49-c0f7-4bdb-ae7c-8e35d28679ac@ushpwbmsmhp002.one.ads.bms.com> Message-ID: Sorry I did not include my contact information. Christina Thurby christina.thurby@bms.com 812-429-8097 Thanks! >-----Original Message----- >From: Thurby, Christina >Sent: Monday, August 24, 2009 9:59 AM >To: 'histonet@lists.utsouthwestern.edu' >Subject: reduction/elimination of red cell autofluorescence on FFPE sections for IF >examination > >Can anyone give feedback on reagents/procedures to use for immunofluorescence to >reduce/eliminate red cell autofluorescence on FFPE sections. I am using an indirect method >of labeling for two antibodies (FITC and Texas Red). > >I have not tried 0.1% sodium borohydride. Will this help for formalin fixed specimens? I >have read that it is used for gluteraldehyde autofluorescence reduction. If appropriate how >long should this reagent be applied to the specimen and at what temperature. This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From portera <@t> msu.edu Mon Aug 24 10:17:00 2009 From: portera <@t> msu.edu (Amy Porter) Date: Mon Aug 24 10:17:11 2009 Subject: [Histonet] RE: reduction/elimination of red cell autofluorescence on FFPE sections for IF examination References: <41f5ad49-c0f7-4bdb-ae7c-8e35d28679ac@ushpwbmsmhp002.one.ads.bms.com> Message-ID: <7643643802EE451DB0FB411A02DCD24D@histolab> We have been utilizing 0.25% Ammonium Hydroxide(28%) + 70% Ethanol v/v for 1 hour followed by 10 minutes in 50% Ethanol rinsing in several changes of buffer afterward and it seems to be working well for a multitude of autofluorescent problems on FFPE sections for us. We tried to Sodium Borohydride and it was a little diffiucult to work with....quite hazardous. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Thurby, Christina" To: "Thurby, Christina" ; Sent: Monday, August 24, 2009 11:00 AM Subject: [Histonet] RE: reduction/elimination of red cell autofluorescence on FFPE sections for IF examination Sorry I did not include my contact information. Christina Thurby christina.thurby@bms.com 812-429-8097 Thanks! >-----Original Message----- >From: Thurby, Christina >Sent: Monday, August 24, 2009 9:59 AM >To: 'histonet@lists.utsouthwestern.edu' >Subject: reduction/elimination of red cell autofluorescence on FFPE >sections for IF >examination > >Can anyone give feedback on reagents/procedures to use for >immunofluorescence to >reduce/eliminate red cell autofluorescence on FFPE sections. I am using an >indirect method >of labeling for two antibodies (FITC and Texas Red). > >I have not tried 0.1% sodium borohydride. Will this help for formalin >fixed specimens? I >have read that it is used for gluteraldehyde autofluorescence reduction. >If appropriate how >long should this reagent be applied to the specimen and at what >temperature. This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ssylvest <@t> cinci.rr.com Mon Aug 24 10:18:52 2009 From: ssylvest <@t> cinci.rr.com (ssylvest@cinci.rr.com) Date: Mon Aug 24 10:18:55 2009 Subject: [Histonet] Procedure for anti-human TCR V alpha24 Message-ID: <20090824151852.BI3NQ.29877.root@hrndva-web20-z01> Hello all, I am working in an Allergy and Immunology research lab and we have been trying to work up a flow cytometry antibody for IHC on paraffin. The antibody is mouse anti-human TCR V alpha24-Biotin from BeckmanCoulter. Does anyone have a working procedure for this antibody? I have tried many dilutions, retrieval methods and incubation times but nothing seems to work. Thanks in advance for your help. Sabina Sylvest CLS II Department of Allergy and Immunology Cincinnati Children's Research Foundation Cincinnati, OH (513)803-0741 ssylvest@cinci.rr.com From alyssa <@t> alliedsearchpartners.com Mon Aug 24 10:30:02 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Mon Aug 24 10:30:11 2009 Subject: [Histonet] Histotech Needed In Dover, FL Area Message-ID: Good Morning/Afternoon Histonetters, Allied Search Partners is currently accepting resumes for our client in the Dover, FL area. We are prescreenign for the following: Position: Histotechnologists/Histotechnicians Shift:Full time/permanent, Day Shift Please submit resume for prescreening purposes to alyssa@alliedsearchpartners.com *All inquiries are kept confidential* Be sure to visit our website to submit a job search request, refer a friend for $$Cash Bonus&&, and submit your resume to one of our career advisors. -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From jwatson <@t> gnf.org Mon Aug 24 10:30:48 2009 From: jwatson <@t> gnf.org (James Watson) Date: Mon Aug 24 10:30:53 2009 Subject: [Histonet] RE: reduction/elimination of red cell autofluorescence on FFPE sections for IF examination In-Reply-To: <7643643802EE451DB0FB411A02DCD24D@histolab> References: <41f5ad49-c0f7-4bdb-ae7c-8e35d28679ac@ushpwbmsmhp002.one.ads.bms.com> <7643643802EE451DB0FB411A02DCD24D@histolab> Message-ID: The copper binds with the hemoglobin in the RBC and greatly reduces or eliminates autofluoresence depending on timing. 10 mM Copper Sulfate 10 mM Copper Sulfate Cupric Sulfate...............1.25 gm 50 mM Amonium acetate (pH5)........500.0 ml Adjust ph to 5.0 with 1.0 M NaOH 50 mM Ammonium acetate (pH5) Ammonium acetate.............1.93 gm Distilled water................500.0 ml Adjust pH to 5.0 with 1.0 M HCl 1. After staining wash slides in Reaction Buffer 2 times for 15 minutes each. 2. Rinse in PBS 2 times for 10 minutes each. 3. Rinse in distilled water 5 minutes. 4. Place slides in 10mM copper sulfate for 5 minutes. 5. Return slides to distilled water and check for autofluorescence with microscope. 6. if needed return slides to 10mM copper sulfate for a couple of more minutes and check again. 7. Rinse slides for 5 minutes in distilled water. 8. Rinse in PBS 2 times for 10 minutes each. 9. Coverslip slides with appropriate mounting media. James Watson HT? ASCP Facilities Manager of Histology GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Porter Sent: Monday, August 24, 2009 8:17 AM To: Thurby, Christina; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: reduction/elimination of red cell autofluorescence on FFPE sections for IF examination We have been utilizing 0.25% Ammonium Hydroxide(28%) + 70% Ethanol v/v for 1 hour followed by 10 minutes in 50% Ethanol rinsing in several changes of buffer afterward and it seems to be working well for a multitude of autofluorescent problems on FFPE sections for us. We tried to Sodium Borohydride and it was a little diffiucult to work with....quite hazardous. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Thurby, Christina" To: "Thurby, Christina" ; Sent: Monday, August 24, 2009 11:00 AM Subject: [Histonet] RE: reduction/elimination of red cell autofluorescence on FFPE sections for IF examination Sorry I did not include my contact information. Christina Thurby christina.thurby@bms.com 812-429-8097 Thanks! >-----Original Message----- >From: Thurby, Christina >Sent: Monday, August 24, 2009 9:59 AM >To: 'histonet@lists.utsouthwestern.edu' >Subject: reduction/elimination of red cell autofluorescence on FFPE >sections for IF >examination > >Can anyone give feedback on reagents/procedures to use for >immunofluorescence to >reduce/eliminate red cell autofluorescence on FFPE sections. I am using an >indirect method >of labeling for two antibodies (FITC and Texas Red). > >I have not tried 0.1% sodium borohydride. Will this help for formalin >fixed specimens? I >have read that it is used for gluteraldehyde autofluorescence reduction. >If appropriate how >long should this reagent be applied to the specimen and at what >temperature. This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Mon Aug 24 10:54:13 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon Aug 24 10:55:43 2009 Subject: [Histonet] refrigerating sliver and gold chloride?? References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2B0A@fhosxchmb006.ADVENTISTCORP.NET> I wonder if 'storing these reagents in the refrigerator' concept originated with the idea of 'keeping them in the dark'. Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of John Kiernan Sent: Fri 8/21/2009 5:00 PM To: Cheri Miller Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] refrigerating sliver and gold chloride?? There was never any reason to refrigerate "gold chloride" or silver nitrate. These compounds (solid or dissolved) can be kept for many years at room temperature. If the solutions are used repeatedly they eventually deteriorate from contamination with bits of sections, causing a changed appearance. Gold solutions take on a greenish grey hue and flakes of metallic gold eventually settle out. These can easily be recovered and recycled to make "gold chloride" (HAuCl4) again. Clean "gold chloride" solutions keep for ever. I have a few bottles of 0.5% that are still that beautiful yellow colour after about 25 years. Old silver nitrate looks a bit grey, not completely colourless. Re-purifying in a histology lab isn't really feasible. You can precipitate out and collect the silver, but (strangely) refining companies don't want it. Your message mentioned "silver and gold chloride". I don't know a histological use for silver chloride, which is very sensitive to light - goes grey-violet as you look at it. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Cheri Miller Date: Friday, August 21, 2009 12:10 Subject: [Histonet] refrigerating sliver and gold chloride?? To: "histonet@lists.utsouthwestern.edu" Cc: "histonet-bounces@lists.utsouthwestern.edu" > I just noticed that my silver and gold chloride says to store at > room temp now. I have always stored them in the refrigerator. Is > anyone still doing this?? Old habits die hard. I want to know if > this has caused any staining issues for anyone. > > Cheryl Miller HT (ASCP) > Histology Supervisor > Physicians Laboratory,P.C. > Omaha, Ne. > 402 738 5052 > > > ________________________________ > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this > message. If you are not the addressee intended / indicated or > agent responsible for delivering it to the addressee, you are > hereby notified that you are in possession of confidential and > privileged information. Any dissemination, distribution, or > copying of this e-mail is strictly prohibited. If you have > received this message in error, please notify the sender > immediately and delete this email from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Tina.Hayes <@t> va.gov Mon Aug 24 11:41:17 2009 From: Tina.Hayes <@t> va.gov (Hayes, Tina J.) Date: Mon Aug 24 11:42:11 2009 Subject: [Histonet] water on slides? Message-ID: <01A34750423B874399B8AF6674D90A270440FB8B@VHAV01MSGA1.v01.med.va.gov> We are having a problem with what seems to be water on our slides. We have had very high humidity levels in the air. It seems that we look at the slides before taking them to the pathology office, one pathologist reviews them and sees little or no water droplets, but as they sit and another pathologist reads them later, like the following day, the droplets are very apparent. We use Clearite-3. We have no xylene in our lab. We also coverslip with Permount. Is it possible that the clearite-3 and/or Permount is absorbing moisture from the atmosphere to the slides? And if so, do you have any suggestions for combating this issue? From jhabecke <@t> fhcrc.org Mon Aug 24 11:58:13 2009 From: jhabecke <@t> fhcrc.org (Randolph-Habecker, Julie) Date: Mon Aug 24 11:58:19 2009 Subject: [Histonet] CD4 and CD8 in FFPE mouse tissue Message-ID: <040346FA7309BD439C327F97D4C4D69B072C7357@ISIS.fhcrc.org> Folks, It's my annual inquiry as to whether someone has optimized immunohistochemistry for CD4 and CD8 on formalin-fixed paraffin mouse tissue. Over the years we have tried lots of different antibodies but I am wondering if there is anything new that we have not tried. Any leads would be great! Thanks, Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org From JCBRITTON <@t> Cheshire-Med.COM Mon Aug 24 12:20:11 2009 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Mon Aug 24 12:20:18 2009 Subject: [Histonet] water on slides? In-Reply-To: <01A34750423B874399B8AF6674D90A270440FB8B@VHAV01MSGA1.v01.med.va.gov> References: <01A34750423B874399B8AF6674D90A270440FB8B@VHAV01MSGA1.v01.med.va.gov> Message-ID: When this happens in our lab, we put Drierite (anhydrous calcium sulfate) into the clearing agents. Just put enough to have not quite a solid layer on the bottom of the staining well, so it will not block the staining rack from going all the way into the clearing agent. We get ours from W.A. Hammond Drierite Company LTD, Xenia, Ohio 45385. Telephone number 937-376-2927. I hope this helps! Josie Britton HT Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hayes, Tina J. Sent: Monday, August 24, 2009 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] water on slides? We are having a problem with what seems to be water on our slides. We have had very high humidity levels in the air. It seems that we look at the slides before taking them to the pathology office, one pathologist reviews them and sees little or no water droplets, but as they sit and another pathologist reads them later, like the following day, the droplets are very apparent. We use Clearite-3. We have no xylene in our lab. We also coverslip with Permount. Is it possible that the clearite-3 and/or Permount is absorbing moisture from the atmosphere to the slides? And if so, do you have any suggestions for combating this issue? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From akbitting <@t> geisinger.edu Mon Aug 24 13:00:36 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Aug 24 13:00:48 2009 Subject: [Histonet] water on slides? In-Reply-To: <01A34750423B874399B8AF6674D90A270440FB8B@VHAV01MSGA1.v01.med.va.gov> References: <01A34750423B874399B8AF6674D90A270440FB8B@VHAV01MSGA1.v01.med.va.gov> Message-ID: <4A929D04.2B7F.00C9.0@geisinger.edu> We are having a very similar problem too. We change our alcohols and Histoclear like mad, but are having eosin bleeding out of the sections. I'm starting to wonder if it is the mounting media on our glass coverslipper. I ordered new bottle gaskets, so I guess I'll see what happens then. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Hayes, Tina J." 8/24/2009 12:41 PM >>> We are having a problem with what seems to be water on our slides. We have had very high humidity levels in the air. It seems that we look at the slides before taking them to the pathology office, one pathologist reviews them and sees little or no water droplets, but as they sit and another pathologist reads them later, like the following day, the droplets are very apparent. We use Clearite-3. We have no xylene in our lab. We also coverslip with Permount. Is it possible that the clearite-3 and/or Permount is absorbing moisture from the atmosphere to the slides? And if so, do you have any suggestions for combating this issue? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From GDawson <@t> dynacaremilwaukee.com Mon Aug 24 13:05:25 2009 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon Aug 24 13:05:37 2009 Subject: [Histonet] Block Verification In-Reply-To: <01A34750423B874399B8AF6674D90A270440FB8B@VHAV01MSGA1.v01.med.va.gov> Message-ID: All, Our histology lab would like to verify that 2 paraffin blocks are from the same person to calm a pathologist's fears. Does anyone know of a good test for this purpose...PCR of some sort perhaps?? Any suggestions would be greatly appreciated. Thank-you In Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From GDawson <@t> dynacaremilwaukee.com Mon Aug 24 13:31:44 2009 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon Aug 24 13:31:50 2009 Subject: [Histonet] IgG4 In-Reply-To: Message-ID: All, Is anyone performing paraffin block IHC staining for IgG4? If so, a vendor name would be much appreciated. Thank-you, Glen Dawson Milwaukee, WI From Rcartun <@t> harthosp.org Mon Aug 24 13:34:29 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Aug 24 13:34:37 2009 Subject: [Histonet] Block Verification In-Reply-To: References: <01A34750423B874399B8AF6674D90A270440FB8B@VHAV01MSGA1.v01.med.va.gov> Message-ID: <4A92A4F5.7400.0077.1@harthosp.org> Our Molecular Pathology Laboratory offers a multiplex PCR assay for identity markers for these situations. They receive specimens from all over the country for this testing (usually specimen mix-ups). It's not cheap and they only bill the referring facility. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Dawson, Glen" 8/24/2009 2:05 PM >>> All, Our histology lab would like to verify that 2 paraffin blocks are from the same person to calm a pathologist's fears. Does anyone know of a good test for this purpose...PCR of some sort perhaps?? Any suggestions would be greatly appreciated. Thank-you In Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janice.Mahoney <@t> alegent.org Mon Aug 24 13:41:40 2009 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Mon Aug 24 13:41:47 2009 Subject: [Histonet] Block Verification In-Reply-To: References: <01A34750423B874399B8AF6674D90A270440FB8B@VHAV01MSGA1.v01.med.va.gov> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDDF6@EXCHMBC2.ad.ah.local> Glen, We had the same situation about a year ago. We sent the block and a bucal smear out for DNA analysis. Now we have the Vantage system to assure positive patient id. Check into it. It saves time and worry and assures patient safety. Jan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Monday, August 24, 2009 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Verification All, Our histology lab would like to verify that 2 paraffin blocks are from the same person to calm a pathologist's fears. Does anyone know of a good test for this purpose...PCR of some sort perhaps?? Any suggestions would be greatly appreciated. Thank-you In Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From christiegowan <@t> msn.com Mon Aug 24 14:38:46 2009 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Mon Aug 24 14:38:50 2009 Subject: [Histonet] Block Verification In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDDF6@EXCHMBC2.ad.ah.local> References: <01A34750423B874399B8AF6674D90A270440FB8B@VHAV01MSGA1.v01.med.va.gov> <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDDF6@EXCHMBC2.ad.ah.local> Message-ID: You would send out for DNA fingerprinting. We have used the Cleveland Clinic for this. Christie > From: Janice.Mahoney@alegent.org > To: GDawson@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu > Date: Mon, 24 Aug 2009 13:41:40 -0500 > Subject: RE: [Histonet] Block Verification > CC: > > Glen, > We had the same situation about a year ago. We sent the block and a bucal smear out for DNA analysis. > Now we have the Vantage system to assure positive patient id. Check into it. It saves time and worry and assures patient safety. > Jan > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen > Sent: Monday, August 24, 2009 1:05 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Block Verification > > All, > > Our histology lab would like to verify that 2 paraffin blocks are from the same person to calm a pathologist's fears. Does anyone know of a good test for this purpose...PCR of some sort perhaps?? Any suggestions would be greatly appreciated. > > Thank-you In Advance, > > Glen Dawson BS, HT & QIHC (ASCP) > IHC Manager > Milwaukee, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vapatpxs <@t> yahoo.com Mon Aug 24 16:30:39 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Mon Aug 24 16:30:42 2009 Subject: [Histonet] water on slides? In-Reply-To: <4A929D04.2B7F.00C9.0@geisinger.edu> Message-ID: <542249.23158.qm@web46110.mail.sp1.yahoo.com> I'm getting the same eosin bleeding and water on the slides. I'm using Hemo-D and CitriSolv. I have changed the reagents and am still getting the eosin bleeding, the water on the slide is a new phenomenon. Any suggestions? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Mon, 8/24/09, Angela Bitting wrote: > From: Angela Bitting > Subject: Re: [Histonet] water on slides? > To: histonet@lists.utsouthwestern.edu, "Tina J. Hayes" > Date: Monday, August 24, 2009, 6:00 PM > We are having a very similar problem > too. We change our alcohols and Histoclear like mad, but are > having eosin bleeding out of the sections. I'm starting to > wonder if it is the mounting media on our glass > coverslipper. I ordered new bottle gaskets, so I guess I'll > see what happens then. > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone? 570-214-9634 > fax? 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > >>> "Hayes, Tina J." > 8/24/2009 12:41 PM >>> > We are having a problem with what seems to be water on our > slides.? We > have had very high humidity levels in the air.? It > seems that we look at > the slides before taking them to the pathology office, one > pathologist > reviews them and sees little or no water droplets, but as > they sit and > another pathologist reads them later, like the following > day, the > droplets are very apparent. > > > > We use Clearite-3.? We have no xylene in our > lab.? We also coverslip > with Permount. > > > > Is it possible that the clearite-3 and/or Permount is > absorbing moisture > from the atmosphere to the slides? > > And if so, do you have any suggestions for combating this > issue? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > IMPORTANT WARNING: The information in this message (and the > documents attached to it, if any) is confidential and may be > legally privileged. It is intended solely for the addressee. > Access to this message by anyone else is unauthorized. If > you are not the intended recipient, any disclosure, copying, > distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you > have received this message in error, please delete all > electronic copies of this message (and the documents > attached to it, if any), destroy any hard copies you may > have created and notify me immediately by replying to this > email. Thank you. > -----Inline Attachment Follows----- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Tony_Reilly <@t> health.qld.gov.au Mon Aug 24 18:14:16 2009 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Mon Aug 24 18:16:05 2009 Subject: [Histonet] water on slides? In-Reply-To: <542249.23158.qm@web46110.mail.sp1.yahoo.com> References: <4A929D04.2B7F.00C9.0@geisinger.edu> <542249.23158.qm@web46110.mail.sp1.yahoo.com> Message-ID: <4A93AB68.471C.0039.0@health.qld.gov.au> If your solutions are clean the cause may be that the level of the water wash station on your stainer is higher than the level of the subsequent alcohol container which means that some water will not be removed from the top of the slide. This is common with running water washes on stainers as the water pressure will elevate the level. A common artifact appears as a blue stripe down the slide caused by the water running down and removing the eosin. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> Va Paula Sicurello 25/08/2009 7:30 am >>> I'm getting the same eosin bleeding and water on the slides. I'm using Hemo-D and CitriSolv. I have changed the reagents and am still getting the eosin bleeding, the water on the slide is a new phenomenon. Any suggestions? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Mon, 8/24/09, Angela Bitting wrote: > From: Angela Bitting > Subject: Re: [Histonet] water on slides? > To: histonet@lists.utsouthwestern.edu, "Tina J. Hayes" > Date: Monday, August 24, 2009, 6:00 PM > We are having a very similar problem > too. We change our alcohols and Histoclear like mad, but are > having eosin bleeding out of the sections. I'm starting to > wonder if it is the mounting media on our glass > coverslipper. I ordered new bottle gaskets, so I guess I'll > see what happens then. > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > >>> "Hayes, Tina J." > 8/24/2009 12:41 PM >>> > We are having a problem with what seems to be water on our > slides. We > have had very high humidity levels in the air. It > seems that we look at > the slides before taking them to the pathology office, one > pathologist > reviews them and sees little or no water droplets, but as > they sit and > another pathologist reads them later, like the following > day, the > droplets are very apparent. > > > > We use Clearite-3. We have no xylene in our > lab. We also coverslip > with Permount. > > > > Is it possible that the clearite-3 and/or Permount is > absorbing moisture > from the atmosphere to the slides? > > And if so, do you have any suggestions for combating this > issue? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > IMPORTANT WARNING: The information in this message (and the > documents attached to it, if any) is confidential and may be > legally privileged. It is intended solely for the addressee. > Access to this message by anyone else is unauthorized. If > you are not the intended recipient, any disclosure, copying, > distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you > have received this message in error, please delete all > electronic copies of this message (and the documents > attached to it, if any), destroy any hard copies you may > have created and notify me immediately by replying to this > email. Thank you. > -----Inline Attachment Follows----- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From c.m.vanderloos <@t> amc.uva.nl Tue Aug 25 02:30:19 2009 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Tue Aug 25 02:30:49 2009 Subject: [Histonet] RE: reduction/elimination of red cell autofluorescence Message-ID: Date: Mon, 24 Aug 2009 10:58:43 -0400 From: "Thurby, Christina" Subject: [Histonet] reduction/elimination of red cell autofluorescence on FFPE sections for IF examination To: "histonet@lists.utsouthwestern.edu" Can anyone give feedback on reagents/procedures to use for immunofluorescence to reduce/eliminate red cell autofluorescence on FFPE sections. I am using an indirect method of labeling for two antibodies (FITC and Texas Red). I have not tried 0.1% sodium borohydride. Will this help for formalin fixed specimens? I have read that it is used for gluteraldehyde autofluorescence reduction. If appropriate how long should this reagent be applied to the specimen and at what temperature. Christina Thurby christina.thurby@bms.com 812-429-8097 ********************************************************************************* Dear Christina,One of the most spectacular ways of getting rid of autofluorescence in FFPE tissue sections is 'unmixing' the autofluorescence from real fluorescence. This can be done with spectral imaging using the Nuance system (http://www.cri-inc.com/applications/fluorescence.asp). Obviously this is not affecting your epitopes with all kinds of nasty chemicals. On top of that, using the Nuance system, the autofluorescence can be pseudo-stained in grey as a kind of 'counterstain'. It's absolutely worth looking at it! Spectral imaging is the subject of workshop #66 at the upcoming NSH convention.Cheers, Chris Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 fax: +31 20 6960389 e-mail: c.m.vanderloos@amc.uva.nl From jshelley <@t> burnham.org Tue Aug 25 05:52:14 2009 From: jshelley <@t> burnham.org (John Shelley) Date: Tue Aug 25 05:52:18 2009 Subject: [Histonet] Question about Ventana IHC stainers Message-ID: Good Morning Histonetters, I would like to know if those of you who have a Ventana Benchmark, Ultra, or Discovery have ever tried to restain slides on the machine and had a problem with inconsistent staining pattern the second time. I have found that multiple sections on a slide do not all stain the second time but had the first. If you have done this please explain how you may have cleaned the slides off and the reagents you may have used after that to clean the slide. I worked on a Dako previously and had never had any difficulties doing this. Thanks for any responses! John From emily <@t> pathlabsolutions.com Tue Aug 25 07:54:22 2009 From: emily <@t> pathlabsolutions.com (Emily Butte) Date: Tue Aug 25 07:54:28 2009 Subject: [Histonet] Sakura Products Message-ID: Hi All: I'm looking to purchase equipment for a new lab and would like the opinion of anyone who has worked with various Sakura products before... good or bad. I would be looking to buy the following products: Tissue-Tek TEC 5 Embedding Station Accu-Cut SRM 200 Microtome Cyto-Tek Cytocentrifuge Also if anyone has experience with prostate and GI bx processed in the Tissue-Tek Paraform Sectional Cassette System, I'd appreciate input on that too. Thanks! -- Emily Butte, M.S., HTL (ASCP) Path Lab Solutions Inc. (701) 371-7515 emily@pathlabsolutions.com From rjbuesa <@t> yahoo.com Tue Aug 25 08:13:27 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 25 08:13:31 2009 Subject: [Histonet] Sakura Products In-Reply-To: Message-ID: <29569.17536.qm@web65706.mail.ac4.yahoo.com> Buying a Sakura instrument is a guarantee for durability and peace of mind. Ren? J. --- On Tue, 8/25/09, Emily Butte wrote: From: Emily Butte Subject: [Histonet] Sakura Products To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 25, 2009, 8:54 AM Hi All: I'm looking to purchase equipment for a new lab and would like the opinion of anyone who has worked with various Sakura products before... good or bad. I would be looking to buy the following products: Tissue-Tek TEC 5 Embedding Station Accu-Cut SRM 200 Microtome Cyto-Tek Cytocentrifuge Also if anyone has experience with prostate and GI bx processed in the Tissue-Tek Paraform Sectional Cassette System, I'd appreciate input on that too. Thanks! -- Emily Butte, M.S., HTL (ASCP) Path Lab Solutions Inc. (701) 371-7515 emily@pathlabsolutions.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Aug 25 08:31:04 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Aug 25 08:31:06 2009 Subject: [Histonet] Sakura Products In-Reply-To: References: Message-ID: <5D64396A0D4A5346BEBC759022AAEAA577E928@ITSSSXM01V6.one.ads.che.org> You will be very pleased with all Sakura products. Excellent company. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Butte Sent: Tuesday, August 25, 2009 08:54 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura Products Hi All: I'm looking to purchase equipment for a new lab and would like the opinion of anyone who has worked with various Sakura products before... good or bad. I would be looking to buy the following products: Tissue-Tek TEC 5 Embedding Station Accu-Cut SRM 200 Microtome Cyto-Tek Cytocentrifuge Also if anyone has experience with prostate and GI bx processed in the Tissue-Tek Paraform Sectional Cassette System, I'd appreciate input on that too. Thanks! -- Emily Butte, M.S., HTL (ASCP) Path Lab Solutions Inc. (701) 371-7515 emily@pathlabsolutions.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From lpaveli1 <@t> hurleymc.com Tue Aug 25 09:21:24 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Tue Aug 25 09:21:39 2009 Subject: [Histonet] lead, barium detection Message-ID: <4A93BB24020000EE0002BDF7@smtp-gw.hurleymc.com> Hello Histonet, Our CSI pathologist wants to detect lead or barium in autopsy tissue already fixed in 10%NBF. Do any of you have any good staining methods you could share? thanks alot, Lynette From shive003 <@t> umn.edu Tue Aug 25 09:31:25 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Aug 25 09:31:40 2009 Subject: [Histonet] glyerin/phenol storage Message-ID: I'm posting this for a colleague.... Does anyone have experience using glycerin/phenol as a storage medium, and if so, for how long are the tissues still good for Histology processing/staining? I assume they can't be used for IHC. Thanks in advance, Jan Shivers University of Minnesota Veterinary Diagnostic Laboratory From annigyg <@t> gmail.com Tue Aug 25 09:56:02 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Tue Aug 25 09:56:09 2009 Subject: [Histonet] Sakura Products In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA577E928@ITSSSXM01V6.one.ads.che.org> References: <5D64396A0D4A5346BEBC759022AAEAA577E928@ITSSSXM01V6.one.ads.che.org> Message-ID: thumbs up for Sakura!! buy them - dont wait for any further advice annieinarabia (out of africa) 2009/8/25 Weems, Joyce > You will be very pleased with all Sakura products. Excellent company. > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily > Butte > Sent: Tuesday, August 25, 2009 08:54 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Sakura Products > > Hi All: > > I'm looking to purchase equipment for a new lab and would like the > opinion of anyone who has worked with various Sakura products before... > good or bad. > I would be looking to buy the following products: > > Tissue-Tek TEC 5 Embedding Station > Accu-Cut SRM 200 Microtome > Cyto-Tek Cytocentrifuge > > Also if anyone has experience with prostate and GI bx processed in the > Tissue-Tek Paraform Sectional Cassette System, I'd appreciate input on > that too. > > Thanks! > > -- > Emily Butte, M.S., HTL (ASCP) > Path Lab Solutions Inc. > (701) 371-7515 > emily@pathlabsolutions.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From alyssa <@t> alliedsearchpartners.com Tue Aug 25 09:56:06 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Aug 25 09:56:12 2009 Subject: [Histonet] Part Time and Full Time Positions In Florida (Melbourne) Message-ID: Allied Search Partners is currently accepting resumes for our client in Melbourne, FL area. We are prescreening for the following: Position(s): Histotechnologists/Histotechnicians Shift: 1. One tech needed for Day Shift Full Time (8am-4:30pm) 2. One tech needed for Part Time Please submit resume for prescreening purposes to alyssa@alliedsearchpartners.com *All inquiries are kept confidential* Be sure to visit our website to submit a job search request, refer a friend for $$Cash Bonus&&, and submit your resume to one of our career advisors. *Other positions in Florida:* Dover, FL (Evening and Overnight Shift Available) -- *Be sure to visit us on the web* www.alliedsearchpartners.com Alyssa Peterson, Director Of Recruitment Allied Search Partners O:888.388.7571 ext. 101 F: 888.388.7572 From mcauliff <@t> umdnj.edu Tue Aug 25 10:24:37 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Aug 25 10:22:59 2009 Subject: [Histonet] glyerin/phenol storage In-Reply-To: References: Message-ID: <4A940235.20504@umdnj.edu> Hi Jan: We used to use glycerine+phenol for storing slices of embalmed cadavers before embedding in clear plastic. I don't know the exact ratio but it was mostly glycerin. We did not stain but painted the nerves, blood vessels, etc as a teaching tool. As for IHC, it would depend on the fixation and the antigen in question. Geoff Jan Shivers wrote: > I'm posting this for a colleague.... > > Does anyone have experience using glycerin/phenol as a storage medium, and if so, for how long are the tissues still good for Histology processing/staining? I assume they can't be used for IHC. > > Thanks in advance, > > Jan Shivers > University of Minnesota > Veterinary Diagnostic Laboratory > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From pvlies <@t> yahoo.com Tue Aug 25 11:05:54 2009 From: pvlies <@t> yahoo.com (Pam V) Date: Tue Aug 25 11:05:57 2009 Subject: [Histonet] Need equipment Message-ID: <646729.78909.qm@web81104.mail.mud.yahoo.com> I am part of the adjunct faculty at Elgin Community College in Elgin Illinois. We are looking for donations to a new histology program and would truly appreciate an embedding center or two. If you can guide me in the direction of one - or two - I'd be a? very happy camper !! We could also use a stainer.. Thanks ! Pam Vlies, HT-ASCP pamela.vlies@shermanhospital.org pvlies@yahoo.com Sherman Hospital Elgin Community College Elgin, IL From Maria.Katleba <@t> stjoe.org Tue Aug 25 11:56:09 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Aug 25 11:56:21 2009 Subject: [Histonet] Trouble making a good Cell Block In-Reply-To: References: <5D64396A0D4A5346BEBC759022AAEAA577E928@ITSSSXM01V6.one.ads.che.org> Message-ID: <97C02552ECB11346877D3E83CF833ABD13D4FA68FB@SJSNT-SCMAIL03.stjoe.org> Hi All, Can anyone give me a good protocol for making a cell block from a non-gyn fluid? (abdominal fluid, bronchial wash, pleural fluid, etc) First of all, we have switched to formalin. Yes! Formalin!! So as you would expect, no button forms. The reason? The pathologists believe the formalin is better than alcohol (95%) especially when you expect to run IHCs on the cell blocks. Please send me ideas!!!! Maria Katleba HT(ASCP) MS Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From DKBoyd <@t> chs.net Tue Aug 25 12:31:18 2009 From: DKBoyd <@t> chs.net (DKBoyd@chs.net) Date: Tue Aug 25 12:28:22 2009 Subject: [Histonet] Trouble making a good Cell Block In-Reply-To: <97C02552ECB11346877D3E83CF833ABD13D4FA68FB@SJSNT-SCMAIL03.stjoe.org> Message-ID: Hi Maria, Are you saying you are using formalin as a preservative for non-gyn's? We collect all aspirations in Plasmalyte which is an electrolytic balance for infusion. All other fluids are collected in a green top, sodium heparinized tube. Bronch specimens are sent in saline. Urine and CSF are sent fresh. When a cell block is requested we centrifuge the sediment to get a button. Pour off the supernate. Then we use HistoGel to make the button. As indicated, HistoGel is a gel you heat up in the microwave. Pour off an equal amount into the non-gyn button let it solidify then you process the solid material just as any other specimen. Hope this helps. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkboyd@chs.net Maria Katleba Sent by: histonet-bounces@lists.utsouthwestern.edu 08/25/2009 01:00 PM To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Trouble making a good Cell Block Hi All, Can anyone give me a good protocol for making a cell block from a non-gyn fluid? (abdominal fluid, bronchial wash, pleural fluid, etc) First of all, we have switched to formalin. Yes! Formalin!! So as you would expect, no button forms. The reason? The pathologists believe the formalin is better than alcohol (95%) especially when you expect to run IHCs on the cell blocks. Please send me ideas!!!! Maria Katleba HT(ASCP) MS Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From WBENTON <@t> umm.edu Tue Aug 25 12:37:40 2009 From: WBENTON <@t> umm.edu (Walter Benton) Date: Tue Aug 25 12:38:08 2009 Subject: [Histonet] Re: Histonet Digest, Vol 69, Issue 26 In-Reply-To: <012E39A4.523@GWIA2.umm.edu> References: <012E39A4.523@GWIA2.umm.edu> Message-ID: <4A93E923.D886.00F4.3@umm.edu> This first thing I would like to know if this tissue has been through a previous pretreatment (antigen retrieval or enzyme digestion) of any kind on the first test, if so I would recommend not using the same slide again for any additional stains. Message: 11 Date: Tue, 25 Aug 2009 06:52:14 -0400 From: John Shelley Subject: [Histonet] Question about Ventana IHC stainers To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Good Morning Histonetters, I would like to know if those of you who have a Ventana Benchmark, Ultra, or Discovery have ever tried to restain slides on the machine and had a problem with inconsistent staining pattern the second time. I have found that multiple sections on a slide do not all stain the second time but had the first. If you have done this please explain how you may have cleaned the slides off and the reagents you may have used after that to clean the slide. I worked on a Dako previously and had never had any difficulties doing this. Thanks for any responses! John Walter Benton Histology Supervisor University of Maryland Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From luke.perkocha <@t> ucsf.edu Tue Aug 25 12:47:09 2009 From: luke.perkocha <@t> ucsf.edu (Perkocha, Luke) Date: Tue Aug 25 12:47:16 2009 Subject: [Histonet] System for Follow-up of Pathology Results Message-ID: Greetings: We have formed a task force to look at "best practices" and system approaches to follow-up of pathology results. Medical errors have resulted when a physician's office has failed to follow-up on an abnormal or unexpected pathology result, or a recommendation made in a pathology report. Sometimes, the report is never received, sometimes it is received, but "lost" in the physician's office, sometimes it is to be discussed with the patient on a follow-up visit and the patient never shows up and the chain of follow-up is broken. We are looking for information on the following: 1. Are there any systems or Anatomic pathology labs out there that have an automated way of confirming that pathology results were received by the ordering physician? 2. Are there any add on systems or middle ware that is in use to do this that can handle multiple different reporting systems: Our labs have 2, Co-path and Intellipath. This may be available in fully integrated medical record and pathology reporting systems, but we are looking for something that works with existing systems. Many thanks. Luke A. Perkocha, MD, MBA Associate Clinical Professor, Pathology and Dermatology Associate Director, Surgical Pathology office: 415 885-7254 e-mail: luke.perkocha@ucsf.edu From jqb7 <@t> cdc.gov Tue Aug 25 13:42:24 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Aug 25 13:42:34 2009 Subject: [Histonet] Sakura Products In-Reply-To: References: Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE101AD360B@LTA3VS011.ees.hhs.gov> We have the embedding center and would highly recommend it -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Butte Sent: Tuesday, August 25, 2009 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura Products Hi All: I'm looking to purchase equipment for a new lab and would like the opinion of anyone who has worked with various Sakura products before... good or bad. I would be looking to buy the following products: Tissue-Tek TEC 5 Embedding Station Accu-Cut SRM 200 Microtome Cyto-Tek Cytocentrifuge Also if anyone has experience with prostate and GI bx processed in the Tissue-Tek Paraform Sectional Cassette System, I'd appreciate input on that too. Thanks! -- Emily Butte, M.S., HTL (ASCP) Path Lab Solutions Inc. (701) 371-7515 emily@pathlabsolutions.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Tue Aug 25 13:44:12 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Aug 25 13:44:18 2009 Subject: [Histonet] lead, barium detection In-Reply-To: <4A93BB24020000EE0002BDF7@smtp-gw.hurleymc.com> References: <4A93BB24020000EE0002BDF7@smtp-gw.hurleymc.com> Message-ID: Lead and barium should end up as their insoluble phosphates in tissues fixed in phosphate-buffered formaldehyde. There are published methods (Pearse's Histochemistry 4th ed Vol 2 [1985]; also in earlier editions, not much different). According to DJ Cook "Cellular Pathology" 2nd ed. Scion 2006, p.174, "It is rare that there is sufficient lead in a normal person to be detectable by histochemical methods. The most sensitive method of detection is the sodium rhodizonate method, which produces a red chelate with lead ions." Barium, strontium and mercury also have red complexes with rhodizonate. Lillie & Fullmer "Histopathologic Technic" 4th ed (1976) pp.547-8 suggest prior treatments to remove salts of Ba and other metals, and adjustment of pH of the rhodizonate solution to enhance specificity for certain metals. My limited experience of staining with rhodizonate for Pb in lab animal tissues (long ago) was rather disappointing, with the result being brownish rather than bright red. I suspect that histochemical staining with rhodizonate for exogenous metals like Ba, Pb, Hg etc is mainly a histochemical curiosity, or am I mistaken? If anyone reading this has significant experience with rhodizonate I would urge them to publish an account, with hints on how to obtain good results every time. I'm sure the Journal of Histotechnology or Biotechnic & Histochemistry would appreciate a good paper on the subject! John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Lynette Pavelich Date: Tuesday, August 25, 2009 10:23 Subject: [Histonet] lead, barium detection To: histonet@lists.utsouthwestern.edu > Hello Histonet, > Our CSI pathologist wants to detect lead or barium in autopsy tissue > already fixed in 10%NBF. Do any of you have any good > staining methods > you could share? > > thanks alot, > Lynette > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fudo <@t> ufl.edu Tue Aug 25 13:58:21 2009 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Tue Aug 25 13:58:24 2009 Subject: [Histonet] FISH probe labeling Message-ID: <1988654.16301251226701303.JavaMail.osg@osgjas02.cns.ufl.edu> Hi, All We are going to do FISH on mouse tissue. But we have to fluorescent label cDNA probe(~2kb in length) by ourselves because there is no commercial probe is available. Does anyone give me some suggestions? Like which company's product is suitable for labeling DNA probe? Which labeling methods is better? End labeling or multi? Thank you, Ann Dongtao Fu Dept. of Pathology University of Flodrida Gainesville, FL 32610 From laurie <@t> conxis.com Tue Aug 25 14:00:21 2009 From: laurie <@t> conxis.com (laurie@conxis.com) Date: Tue Aug 25 14:00:39 2009 Subject: [Histonet] Caspase 3 staining in frozen tissue Message-ID: <0D14B3EC2C9B4897BCCDEB2F9D3F4600.MAI@accuwebhosting.biz> Hello everyone, Is there anyone out there doing Caspase 3 IHC on frozen tissue? I am attempting to restandardize our protocol because the antibody we were using has stopped working. We are using acetone fixed frozen human tonsil. 4% PFA postfixation for 10 minutes. Using a rabbit polyclonal antibody from Abcam and Dako reagents on an autostainer. Thanks for your ideas because mine aren't staining :-(. Laurie From fudo <@t> ufl.edu Tue Aug 25 14:12:54 2009 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Tue Aug 25 14:12:59 2009 Subject: [Histonet] bubbles under coverslip Message-ID: <883841429.17431251227574555.JavaMail.osg@osgjas02.cns.ufl.edu> Hi, all Recently we met a problem of bubbles under coverslip after we changed to Xylene Substitute(from Shandon) instead of Xylene. The bubbles immediately appear after coverslipping. At very beginning, we thought it was due to mounting medium. So we changed to Shandon Xylene Substitue Mountant, but it did not help. Right now we have to transfer slides from Xylene Substitute to xylene, then coverslip them. We hate the extra xylene step! Does anyone know what cause the bubbles? And how to avoid it? Any suggestions will be appreciated. Ann Dongtao Fu Dept. of Pathology University of Flodrida Gainesville, FL 32610 From fudo <@t> ufl.edu Tue Aug 25 14:13:59 2009 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Tue Aug 25 14:14:04 2009 Subject: [Histonet] bubbles under coverslip Message-ID: <850778548.17581251227639927.JavaMail.osg@osgjas02.cns.ufl.edu> Hi, all Recently we met a problem of bubbles under coverslip after we changed to Xylene Substitute(from Shandon) instead of Xylene. The bubbles immediately appear after coverslipping. At very beginning, we thought it was due to mounting medium. So we changed to Shandon Xylene Substitue Mountant, but it did not help. Right now we have to transfer slides from Xylene Substitute to xylene, then coverslip them. We hate the extra xylene step! Does anyone know what cause the bubbles? And how to avoid it? Any suggestions will be appreciated. Ann Dongtao Fu Dept. of Pathology University of Flodrida Gainesville, FL 32610 From fudo <@t> ufl.edu Tue Aug 25 14:15:48 2009 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Tue Aug 25 14:15:52 2009 Subject: [Histonet] bubbles under coverslip Message-ID: <1971575205.17741251227748090.JavaMail.osg@osgjas02.cns.ufl.edu> Hi, all Recently we met a problem of bubbles under coverslip after we changed to Xylene Substitute(from Shandon) instead of Xylene. The bubbles immediately appear after coverslipping. At very beginning, we thought it was due to mounting medium. So we changed to Shandon Xylene Substitue Mountant, but it did not help. Right now we have to transfer slides from Xylene Substitute to xylene, then coverslip them. We hate the extra xylene step! Does anyone know what cause the bubbles? And how to avoid it? Any suggestions will be appreciated. Ann Dongtao Fu Dept. of Pathology University of Flodrida Gainesville, FL 32610 From jshelley <@t> burnham.org Tue Aug 25 14:47:39 2009 From: jshelley <@t> burnham.org (John Shelley) Date: Tue Aug 25 14:47:45 2009 Subject: [Histonet] Question about Ventana IHC stainers Message-ID: Hello Histonetters, I would like to know if those of you who have a Ventana Benchmark, Ultra, or Discovery have ever tried to restain slides on the machine and had a problem with inconsistent staining pattern the second time. I have found that multiple sections on a slide do not all stain the second time but had the first. If you have done this please explain how you may have cleaned the slides off and the reagents you may have used after that to clean the slide. I worked on a Dako previously and had never had any difficulties doing this. Thanks for any responses! John From dlent2 <@t> nycap.rr.com Tue Aug 25 14:49:59 2009 From: dlent2 <@t> nycap.rr.com (Michael & Dominga Lent) Date: Tue Aug 25 14:50:17 2009 Subject: [Histonet] (no subject) Message-ID: <711187079AE64F16A5749750241FE0A7@mia> please take me off list From cbrya <@t> lexclin.com Tue Aug 25 15:02:26 2009 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Tue Aug 25 15:02:32 2009 Subject: [Histonet] Histogel Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF172B39AA@EXCHANGESB> Hello everyone! If you are using Histogel for your cytology cell blocks, how do you liquify it? Do you use an incubator or microwave? How happy are the pathologists with the quality of the cell blocks? Thanks in advance for your information. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From talulahgosh <@t> gmail.com Tue Aug 25 15:03:18 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Aug 25 15:03:22 2009 Subject: [Histonet] (no subject) In-Reply-To: <711187079AE64F16A5749750241FE0A7@mia> References: <711187079AE64F16A5749750241FE0A7@mia> Message-ID: Jupiter's thunder, don't you people ever learn--unsubscribe yourself in the url at the bottom of histonet emails Emily "One of the defining characteristics of modern surgery was that patients ought to survive it." --Peter Stanley, For Fear of Pain: British Surgery, 1790-1850 On Tue, Aug 25, 2009 at 3:49 PM, Michael & Dominga Lent wrote: > please take me off list > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Donna.Millard <@t> prlnet.com Tue Aug 25 15:19:52 2009 From: Donna.Millard <@t> prlnet.com (Donna Millard) Date: Tue Aug 25 15:19:31 2009 Subject: [Histonet] AB&T systems Message-ID: <3D13881C1B0D70488A04E6019B0EAAA2030AAE53@prlexch01> Hello, We are investigating purchasing an Automated Bar Code and Tracking System. So far we're looking at Ventana's Vantage system, and Cerner CoPath's system. Is anyone aware of other systems out there? We're not looking for just the bar-coding, we also want the tracking features. Thanks Donna Millard, B.S. Histology Supervisor Physicians Reference Laboratory, LLC 7800 W. 110th Street Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070. From akbitting <@t> geisinger.edu Tue Aug 25 15:43:30 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Aug 25 15:43:40 2009 Subject: [Histonet] thermometer vendor Message-ID: <4A9414B1.2B7F.00C9.0@geisinger.edu> Can anyone help me find where to order the short thermometers for use in the floatation baths? Thanks. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From JEllin <@t> yumaregional.org Tue Aug 25 16:06:46 2009 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Aug 25 16:07:01 2009 Subject: [Histonet] AB&T systems In-Reply-To: <3D13881C1B0D70488A04E6019B0EAAA2030AAE53@prlexch01> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8021C5C76@EXCHANGECLUSTER.yumaregional.local> There are several ones that are out there, Ventana-vantage, Dako-TPID, Cerner's solution, There is also Omintrax, but the majority of the solutions are primarly barcode with some tracking feature.. I would ask you if you have an LIS to communicate see if they would interface to a third party vendor,, this usually costs extra. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna Millard Sent: Tuesday, August 25, 2009 1:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AB&T systems Hello, We are investigating purchasing an Automated Bar Code and Tracking System. So far we're looking at Ventana's Vantage system, and Cerner CoPath's system. Is anyone aware of other systems out there? We're not looking for just the bar-coding, we also want the tracking features. Thanks Donna Millard, B.S. Histology Supervisor Physicians Reference Laboratory, LLC 7800 W. 110th Street Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. From Maria.Katleba <@t> stjoe.org Tue Aug 25 16:32:29 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Tue Aug 25 16:32:46 2009 Subject: [Histonet] RE: Trouble making a good Cell Block In-Reply-To: <97C02552ECB11346877D3E83CF833ABD13D4FA68FB@SJSNT-SCMAIL03.stjoe.org> References: <5D64396A0D4A5346BEBC759022AAEAA577E928@ITSSSXM01V6.one.ads.che.org> <97C02552ECB11346877D3E83CF833ABD13D4FA68FB@SJSNT-SCMAIL03.stjoe.org> Message-ID: <97C02552ECB11346877D3E83CF833ABD13D535389A@SJSNT-SCMAIL03.stjoe.org> THANK YOU to everyone out there! There were so many responses; I would have been typing my 'thank yous' all day. You can use my AOL account to send me individual messages/protocols. I do appreciate it! Kind regards, Maria Katleba HT(ASCP) MS Pathology Dept. Napa CA Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From talulahgosh <@t> gmail.com Tue Aug 25 16:44:08 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Aug 25 16:44:14 2009 Subject: [Histonet] thermometer vendor In-Reply-To: <4A9414B1.2B7F.00C9.0@geisinger.edu> References: <4A9414B1.2B7F.00C9.0@geisinger.edu> Message-ID: We ordered ours through Fisher, though I'm not sure how short you need them to be. I remember it took a while to find them on the site, though, since I didn't have the exact length to put in their search engine. Try this though *http://tinyurl.com/mlk3ho* Emily "One of the defining characteristics of modern surgery was that patients ought to survive it." --Peter Stanley, For Fear of Pain: British Surgery, 1790-1850 On Tue, Aug 25, 2009 at 4:43 PM, Angela Bitting wrote: > Can anyone help me find where to order the short thermometers for use in > the floatation baths? Thanks. > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. It is > intended solely for the addressee. Access to this message by anyone else is > unauthorized. If you are not the intended recipient, any disclosure, > copying, distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you have received this > message in error, please delete all electronic copies of this message (and > the documents attached to it, if any), destroy any hard copies you may have > created and notify me immediately by replying to this email. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From mike <@t> pathview.com Tue Aug 25 19:18:09 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Tue Aug 25 19:18:42 2009 Subject: [Histonet] AB&T systems In-Reply-To: <3D13881C1B0D70488A04E6019B0EAAA2030AAE53@prlexch01> References: <3D13881C1B0D70488A04E6019B0EAAA2030AAE53@prlexch01> Message-ID: <009a01ca25e2$b6920560$23b61020$@com> Donna, if you're looking at LIS vendors, which I assume you are, since you're looking at Cerner's Copath, may I ask you to consider our information system. From reading through the literature, we may have been the first commercially available, vendor supplied information system that included complete barcoding and tracking from beginning to end. If you purchase the interface to the dictation system, you never have to enter a case # in our system. Please feel free to contact me offline if you'd like. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna Millard Sent: Tuesday, August 25, 2009 3:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AB&T systems Hello, We are investigating purchasing an Automated Bar Code and Tracking System. So far we're looking at Ventana's Vantage system, and Cerner CoPath's system. Is anyone aware of other systems out there? We're not looking for just the bar-coding, we also want the tracking features. Thanks Donna Millard, B.S. Histology Supervisor Physicians Reference Laboratory, LLC 7800 W. 110th Street Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Aug 25 19:58:20 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Aug 25 19:58:37 2009 Subject: [Histonet] RE: Trouble making a good Cell Block In-Reply-To: <97C02552ECB11346877D3E83CF833ABD13D535389A@SJSNT-SCMAIL03.stjoe.org> Message-ID: Maria, I did not know whether you had many responses. It seems that our Histonetters are forgetting to "Reply to all" so that their valued knowledge is made available to all of us. Please everyone, reply to all not just the sender Thanks Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria Katleba Sent: Wednesday, 26 August 2009 7:32 AM To: histonet@lists.utsouthwestern.edu; mariakatleba@aol.com Subject: [Histonet] RE: Trouble making a good Cell Block THANK YOU to everyone out there! There were so many responses; I would have been typing my 'thank yous' all day. You can use my AOL account to send me individual messages/protocols. I do appreciate it! Kind regards, Maria Katleba HT(ASCP) MS Pathology Dept. Napa CA Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From tifei <@t> foxmail.com Wed Aug 26 01:14:39 2009 From: tifei <@t> foxmail.com (TF) Date: Wed Aug 26 01:45:34 2009 Subject: [Histonet] Caspase 3 staining in frozen tissue References: <0D14B3EC2C9B4897BCCDEB2F9D3F4600.MAI@accuwebhosting.biz> Message-ID: <200908261414383414040@foxmail.com> YWNldG9uZSBmaXhlZCAtPiBQRkEgZml4YXRpb24gYWdhaW4/DQpJIHRoaW5rIG9uZSBzdGVwIGlz IGVub3VnaA0KDQoNCjIwMDktMDgtMjYgDQoNCg0KDQpURiANCg0KDQoNCreivP7Iy6O6IGxhdXJp ZUBjb254aXMuY29tIA0Kt6LLzcqxvOSjuiAyMDA5LTA4LTI2ICAwMzowNDo0MSANCsrVvP7Iy6O6 IGhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdSANCrOty82juiANCtb3zOKjuiBbSGlz dG9uZXRdIENhc3Bhc2UgMyBzdGFpbmluZyBpbiBmcm96ZW4gdGlzc3VlIA0KSGVsbG8gZXZlcnlv bmUsIA0KSXMgdGhlcmUgYW55b25lIG91dCB0aGVyZSBkb2luZyBDYXNwYXNlIDMgSUhDIG9uIGZy b3plbiB0aXNzdWU/ICBJIGFtIGF0dGVtcHRpbmcgdG8gcmVzdGFuZGFyZGl6ZSBvdXIgcHJvdG9j b2wgYmVjYXVzZSB0aGUgYW50aWJvZHkgd2Ugd2VyZSB1c2luZyBoYXMgc3RvcHBlZCB3b3JraW5n LiAgV2UgYXJlIHVzaW5nIGFjZXRvbmUgZml4ZWQgZnJvemVuIGh1bWFuIHRvbnNpbC4gNCUgUEZB IHBvc3RmaXhhdGlvbiBmb3IgMTAgbWludXRlcy4gIFVzaW5nIGEgcmFiYml0IHBvbHljbG9uYWwg YW50aWJvZHkgZnJvbSBBYmNhbSBhbmQgRGFrbyByZWFnZW50cyBvbiBhbiBhdXRvc3RhaW5lci4N ClRoYW5rcyBmb3IgeW91ciBpZGVhcyBiZWNhdXNlIG1pbmUgYXJlbid0IHN0YWluaW5nIDotKC4N CkxhdXJpZSANCl9fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19f DQpIaXN0b25ldCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVk dQ0KaHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3Rv bmV0DQo= From annigyg <@t> gmail.com Wed Aug 26 05:58:52 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed Aug 26 05:58:57 2009 Subject: [Histonet] Sakura Products In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE101AD360B@LTA3VS011.ees.hhs.gov> References: <9A16CB5D55FC1648ADF11B63E72A1BE101AD360B@LTA3VS011.ees.hhs.gov> Message-ID: we have the Embedding centre, DRS2000 stainer, 2 VIP5's, a tape coverslipper and 2 microtomes - all fantastic, dependable, easy-to-use, workhorses - need I say more - this is SAKURA - the name speaks for itself!!! 2009/8/25 Bartlett, Jeanine (CDC/CCID/NCZVED) > We have the embedding center and would highly recommend it > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily > Butte > Sent: Tuesday, August 25, 2009 8:54 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Sakura Products > > Hi All: > > I'm looking to purchase equipment for a new lab and would like the > opinion of anyone who has worked with various Sakura products before... > good or bad. > I would be looking to buy the following products: > > Tissue-Tek TEC 5 Embedding Station > Accu-Cut SRM 200 Microtome > Cyto-Tek Cytocentrifuge > > Also if anyone has experience with prostate and GI bx processed in the > Tissue-Tek Paraform Sectional Cassette System, I'd appreciate input on > that too. > > Thanks! > > -- > Emily Butte, M.S., HTL (ASCP) > Path Lab Solutions Inc. > (701) 371-7515 > emily@pathlabsolutions.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From DStillings <@t> unipathllc.com Wed Aug 26 06:46:18 2009 From: DStillings <@t> unipathllc.com (Donella Stillings) Date: Wed Aug 26 06:46:22 2009 Subject: [Histonet] Sakura Products In-Reply-To: References: Message-ID: <8785CEF5DCC41A4F8014179C435935D60C349F@exchange.unipathllc.corp> Emily, We have 7 VIP5 processors, 2 new 120x express processors. 3 embedding centers, 2 microtomes and the DRS2000 H&E stainer with attached tape coverslipper. All are awesome! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Butte Sent: Tuesday, August 25, 2009 6:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura Products Hi All: I'm looking to purchase equipment for a new lab and would like the opinion of anyone who has worked with various Sakura products before... good or bad. I would be looking to buy the following products: Tissue-Tek TEC 5 Embedding Station Accu-Cut SRM 200 Microtome Cyto-Tek Cytocentrifuge Also if anyone has experience with prostate and GI bx processed in the Tissue-Tek Paraform Sectional Cassette System, I'd appreciate input on that too. Thanks! -- Emily Butte, M.S., HTL (ASCP) Path Lab Solutions Inc. (701) 371-7515 emily@pathlabsolutions.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Wed Aug 26 08:23:23 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Wed Aug 26 08:23:29 2009 Subject: [Histonet] RE: Trouble making a good Cell Block In-Reply-To: References: Message-ID: <3F7B88730225DE6B475B6651@CDYwxp1931.ad.med.buffalo.edu> I agree; it would have been good to see some of these responses posted to Histonet. I monitor the 'net for the purpose of learning the answers to specific questions that others end up posting first, besides adding to my own personal database of general valuable technical knowledge. So hitting the "reply all" button would definitely help. :-) Merced --On Wednesday, August 26, 2009 10:58 AM +1000 Tony Henwood wrote: > Maria, > > I did not know whether you had many responses. > It seems that our Histonetters are forgetting to "Reply to all" so that > their valued knowledge is made available to all of us. > > Please everyone, reply to all not just the sender > > Thanks > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria > Katleba > Sent: Wednesday, 26 August 2009 7:32 AM > To: histonet@lists.utsouthwestern.edu; mariakatleba@aol.com > Subject: [Histonet] RE: Trouble making a good Cell Block > > > THANK YOU to everyone out there! > > There were so many responses; I would have been typing my 'thank yous' > all day. You can use my AOL account to send me individual > messages/protocols. I do appreciate it! > > Kind regards, > > > Maria Katleba HT(ASCP) MS > Pathology Dept. > Napa CA > > > > > > Notice from St. Joseph Health System: > Please note that the information contained in this message may be > privileged and confidential and protected from disclosure. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient, please delete it and > notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The > Childrens Hospital at Westmead accepts no liability for any consequential > damage resulting from email containing computer viruses. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Joseph.Jurcisek <@t> nationwidechildrens.org Wed Aug 26 08:29:22 2009 From: Joseph.Jurcisek <@t> nationwidechildrens.org (Jurcisek, Joseph) Date: Wed Aug 26 08:31:04 2009 Subject: [Histonet] FISH probe labeling In-Reply-To: <1988654.16301251226701303.JavaMail.osg@osgjas02.cns.ufl.edu> References: <1988654.16301251226701303.JavaMail.osg@osgjas02.cns.ufl.edu> Message-ID: <20CE7246011C12489BA04BEB85CCDA1F01CE5CDD3D@RES2K7MS01.CRII.ORG> I have used the ULYSIS Nucleic Acid Labeling Kit from Invitrogen. They make it with many different fluorochromes, I have used the AlexaFluor 488 and AlexaFluor 594 with good results. Joseph A. Jurcisek Senior Research Associate Center for Microbial Pathogenesis The Research Institute at Nationwide Children's Hospital 700 Children's Dr. Columbus, OH 43205-2696 (614) 355-3521 fax (614) 722-2818 Joseph.Jurcisek@nationwidechildrens.org -----Original Message----- From: FU,DONGTAO [mailto:fudo@ufl.edu] Sent: Tuesday, August 25, 2009 2:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FISH probe labeling Hi, All We are going to do FISH on mouse tissue. But we have to fluorescent label cDNA probe(~2kb in length) by ourselves because there is no commercial probe is available. Does anyone give me some suggestions? Like which company's product is suitable for labeling DNA probe? Which labeling methods is better? End labeling or multi? Thank you, Ann Dongtao Fu Dept. of Pathology University of Flodrida Gainesville, FL 32610 ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Janice.Mahoney <@t> alegent.org Wed Aug 26 08:32:40 2009 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Aug 26 08:32:49 2009 Subject: [Histonet] RE: AB&T systems In-Reply-To: <3D13881C1B0D70488A04E6019B0EAAA2030AAE53@prlexch01> References: <3D13881C1B0D70488A04E6019B0EAAA2030AAE53@prlexch01> Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE0E@EXCHMBC2.ad.ah.local> Hi Donna, I have looked into the systems and if you want more than a bar code and tracking system look into Vantage. It is what we chose and we are very happy with it. It will track and trend quality issues, send special instructions about a case to different workstations, the list goes on and on. Vantage is the most comprehensive most in depth system out there in my opinion. Jan Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna Millard Sent: Tuesday, August 25, 2009 3:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AB&T systems Hello, We are investigating purchasing an Automated Bar Code and Tracking System. So far we're looking at Ventana's Vantage system, and Cerner CoPath's system. Is anyone aware of other systems out there? We're not looking for just the bar-coding, we also want the tracking features. Thanks Donna Millard, B.S. Histology Supervisor Physicians Reference Laboratory, LLC 7800 W. 110th Street Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From WBENTON <@t> umm.edu Wed Aug 26 08:39:56 2009 From: WBENTON <@t> umm.edu (Walter Benton) Date: Wed Aug 26 08:40:21 2009 Subject: [Histonet] Tracking system for Histology In-Reply-To: <4E0059A4.764@GWIA1.umm.edu> References: <4E0059A4.764@GWIA1.umm.edu> Message-ID: <4A9502EB.D886.00F4.3@umm.edu> Dako has a positive ID system as well. Walter Benton Histology Supervisor University of Maryland Anatomic Pathology 22 S. Greene St Room NBW65 Baltimore MD 21201 (Direct) 410-328-0930 (Lab) 410-328-5524 (Fax) 410-328-5508 This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From PMonfils <@t> Lifespan.org Wed Aug 26 09:31:38 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Aug 26 09:31:43 2009 Subject: [Histonet] Trouble making a good Cell Block In-Reply-To: <97C02552ECB11346877D3E83CF833ABD13D4FA68FB@SJSNT-SCMAIL03.stjoe.org> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CDC@LSRIEXCH1.lsmaster.lifespan.org> To make a cell block from formalin-fixed cells, you need to introduce some coagulable material that will stick the cells together. Here's a method I have used successfully: Spin down the cells in formalin, decant as much formalin as possible with a pipette. Fill tube with isotonic buffer, resuspend cells, respin, decant as above. Add about 1 ml of isotonic buffer, plus a few drops of coagulant. A concentrated solution of albumin or agar or gelatin can be used, or serum. I'm sorry I don't have exact percentages for solutions. Resuspend, respin, decant as above. Gently add 1 ml of 70% ethanol. Don't drop it directly on the pellet, but allow it to run slowly down the side of the tube. Leave for 20 minutes to 1 hour depending on the size of the pellet. Decant and replace with 95% ethanol, allow to stand as above, replace with absolute ethanol. As the pellet is dehydrated it shrinks, just like any tissue, and usually detaches from the tube once dehydration is complete. Otherwise you can reach down and just touch the pellet with the tip of a probe and it will detach. Then you can either complete hand processing in the tube, or remove the pellet for machine processing. From ryaskovich <@t> dir.nidcr.nih.gov Wed Aug 26 09:35:33 2009 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Wed Aug 26 09:35:41 2009 Subject: [Histonet] Tetramethyl-benzidine Message-ID: Has anyone used this chromagen? I want to use it with N52. Thanks for any help. Ruth Yaskovich N.I.H. From pattennj <@t> mail.nih.gov Wed Aug 26 10:03:08 2009 From: pattennj <@t> mail.nih.gov (Patten, Nicole (NIH/NIAAA) [F]) Date: Wed Aug 26 10:03:34 2009 Subject: [Histonet] Luxol Fast Blue with IHC In-Reply-To: References: Message-ID: Hello- Has anyone tried to counterstain an IHC stain with Luxol Fast Blue? I am worried that the LFB destain will remove some of the IHC. I use the VIP solution from Vector as the chromogen for IHC instead of DAB and I destain Luxol Fast Blue with Hydroquinone/Sodium Sulfite (in addition to the ethanol steps). I'm staining for Ki-67 and I'm doing this on FFPE human brain sections. I haven't found anything in the Histonet archives on this topic. Any advice would be greatly appreciated. Thanks in advance! -Nicole, Post-bacccalaureate Fellow, NIH From shshaw <@t> WPI.EDU Wed Aug 26 10:09:01 2009 From: shshaw <@t> WPI.EDU (Shaw, Sharon) Date: Wed Aug 26 10:08:27 2009 Subject: [Histonet] Anti-NuMA (Ab-2) Mouse mAb (107-7) Calbiochem cat no. NA09L Message-ID: Hello, I have recently began using the Anti-NuMA primary antibody to distinguish the human mesechymal stem cells that I am delivering to the rat heart. Anti-NuMA recognizes human nuclear mitotic apparatus protein. I have tried two secondary antibodies: Alexa Fluor 488 and FITC (Jackson immunoresearch code 715-095-150) and have positively stained human mesenchymal stem cells put on glass coverslips with both secondary antibodies. However, when I look at the sections of my rat heart that I have delivered human mesenchymal stem cells to, I get a lot of autofluorescence and no positive signal (counterstained with Hoechst and the hMSCs are Quantum Dot loaded). The sections were fixed in 4% paraformaldehyde right after the animal was euthanized and heart removed. The heart was then frozen in OCT and sectioned. I have tried changing the concentration of both the secondary's and primary's already (within the manufacturer's specs). My question is: what secondary antibodies do you recommend? Does anyone have experience with this primary? Thanks, Sharon WPI From trathborne <@t> somerset-healthcare.com Wed Aug 26 10:56:50 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Aug 26 10:56:59 2009 Subject: [Histonet] H & E staining Message-ID: Hello, I was wondering if those of you who are using H & E stainers with heating capabilities/ovens could tell me what your average run time is. What instrument are you using? and is the length of time affected by the number of racks being stained? Thank you, Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville,NJ 08876 908-595-2367 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From azdudley <@t> hotmail.com Wed Aug 26 11:24:41 2009 From: azdudley <@t> hotmail.com (anita dudley) Date: Wed Aug 26 11:26:14 2009 Subject: [Histonet] Sakura Products In-Reply-To: References: Message-ID: emily, we have a sakura processor, embedding center, an accu-cut SRM microtome and a coverslipper. they are all great. you can't go wrong with sakura. anita > Date: Tue, 25 Aug 2009 08:54:22 -0400 > From: emily@pathlabsolutions.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Sakura Products > > Hi All: > > I'm looking to purchase equipment for a new lab and would like the opinion > of anyone who has worked with various Sakura products before... good or bad. > I would be looking to buy the following products: > > Tissue-Tek TEC 5 Embedding Station > Accu-Cut SRM 200 Microtome > Cyto-Tek Cytocentrifuge > > Also if anyone has experience with prostate and GI bx processed in the > Tissue-Tek Paraform Sectional Cassette System, I'd appreciate input on that > too. > > Thanks! > > -- > Emily Butte, M.S., HTL (ASCP) > Path Lab Solutions Inc. > (701) 371-7515 > emily@pathlabsolutions.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009 From mlbukhar <@t> ucalgary.ca Wed Aug 26 11:59:56 2009 From: mlbukhar <@t> ucalgary.ca (maureen bukhari) Date: Wed Aug 26 12:00:22 2009 Subject: [Histonet] RE: Histonet Digest, Vol 69, Issue 7 In-Reply-To: <20090807170105.1D7F974003@mhub4.UCALGARY.CA> References: <20090807170105.1D7F974003@mhub4.UCALGARY.CA> Message-ID: <000901ca266e$a3acf680$eb06e380$@ca> I just purchased some from the VWR Catalogue, page 538, top left hand corner, disposable and reusable, 2009-2010 catalogue and you can access it online. Cheers, Maureen Bukhari Phone: 403-210-6524 e-mail: mlbukhar@ucalgary.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, August 07, 2009 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 69, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Core unit support (Va Paula Sicurello) 2. metal embedding base molds (Victoria Baker) 3. Support for core units (Va Paula Sicurello) 4. Re: metal embedding base molds (Amy Porter) 5. Mycoplasma bovis (Breeden, Sara) 6. RE: metal embedding base molds (Bernice Frederick) 7. Re: Mycoplasma bovis (Jenee.S.Odani@hawaii.gov) 8. (no subject) (Maria Katleba) 9. RE: metal embedding base molds (Nancy Klemme) 10. RE: metal embedding base molds (Jodie Robertson) 11. cement in bone specimen (DiCarlo, Margaret) 12. Re: Histonet Digest, Vol 69, Issue 6 (Rick.Garnhart@memorialhealthsystem.com) 13. What do you use for decolorizing after Hematox. (Michelle MacVeigh-Aloni) 14. RE: cement in bone specimen (Jack Ratliff) 15. Fetal demise placenta (mtitford@aol.com) 16. HPGD, PAFa and frizzled-4 stainings on Ventana Benchmark (Vesterinen Tiina) ---------------------------------------------------------------------- Message: 1 Date: Thu, 6 Aug 2009 10:12:31 -0700 (PDT) From: Va Paula Sicurello Subject: [Histonet] Core unit support To: MSA BB , HistoNet Message-ID: <554043.34139.qm@web46112.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello Everybody, I was wondering what type of institutional support y'all have at your various institutions. I work for a research foundation and they want to drop my salary and have the researchers grants pay my salary and almost everything else. I am a fee-for-service lab and the CEO wants to see $25K in recharges between now and December. We offer TEM, confocal, histology and cryosectioning. We will be adding micro-CT and a deconvolution microscope that does FRET and can handle samples that have been transfected(?) with viral vectors. Is she being unrealistic? I am only 80% time and the only person down here. Should I start looking for a new position before she shuts me down? How many people have had their facilities closed by the people who don't see the big picture? Please let me know your experiences. Thanks, Paula :-} Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. ------------------------------ Message: 2 Date: Thu, 6 Aug 2009 13:13:06 -0400 From: Victoria Baker Subject: [Histonet] metal embedding base molds To: histonet Message-ID: <4f016b690908061013r6a410546j8a867e9813392a69@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi- I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm able to find at this point are the metal molds with the 'wings' on them. Does anyone know a source? With all the mergers etc it's been a little challenging. Thanks in advance Vikki ------------------------------ Message: 3 Date: Thu, 6 Aug 2009 10:14:19 -0700 (PDT) From: Va Paula Sicurello Subject: [Histonet] Support for core units To: MSA BB , HistoNet Message-ID: <73199.21406.qm@web46104.mail.sp1.yahoo.com> Content-Type: text/plain; charset=us-ascii Hello Everybody, I was wondering what type of institutional support y'all have at your various institutions. I work for a research foundation and they want to drop my salary and have the researchers grants pay my salary and almost everything else. I am a fee-for-service lab and the CEO wants to see $25K in recharges between now and December. We offer TEM, confocal, histology and cryosectioning. We will be adding micro-CT and a deconvolution microscope that does FRET and can handle samples that have been transfected(?) with viral vectors. Is she being unrealistic? I am only 80% time and the only person down here. Should I start looking for a new position before she shuts me down? How many people have had their facilities closed by the people who don't see the big picture? Please let me know your experiences. Thanks, Paula :-} Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. ------------------------------ Message: 4 Date: Thu, 6 Aug 2009 13:30:34 -0400 From: "Amy Porter" Subject: Re: [Histonet] metal embedding base molds To: "Victoria Baker" , "histonet" Message-ID: <08DEAF2AEF394D02817D86F01AD998AE@histolab> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Try EM Sciences the distribute alot of the unique old type histology supplies. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Victoria Baker" To: "histonet" Sent: Thursday, August 06, 2009 1:13 PM Subject: [Histonet] metal embedding base molds > Hi- > > I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All > I'm > able to find at this point are the metal molds with the 'wings' on them. > > Does anyone know a source? With all the mergers etc it's been a little > challenging. > > Thanks in advance > > Vikki > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 5 Date: Thu, 6 Aug 2009 12:08:44 -0600 From: "Breeden, Sara" Subject: [Histonet] Mycoplasma bovis To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B02E46A05@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="US-ASCII" My boss has thought up a new project for me so I need to know what I need to know about Mycoplasma bovis for IHC. He wants me to cost out the procedure. I normally do only TWO IHCs - CWD and BVD - so I'll be charting new mental territory with this one. Can anyone guide me on where to start figuring this out? Where do I get antibody? Is there a pre-existing protocol for the Ventana NexES? I don't believe in re-inventing the wheel if I don't need to. Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 6 Date: Thu, 6 Aug 2009 13:10:38 -0500 From: "Bernice Frederick" Subject: RE: [Histonet] metal embedding base molds To: "'Amy Porter'" , "'Victoria Baker'" , "'histonet'" Message-ID: Content-Type: text/plain; charset="us-ascii" As well as surgipath. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Porter Sent: Thursday, August 06, 2009 12:31 PM To: Victoria Baker; histonet Subject: Re: [Histonet] metal embedding base molds Try EM Sciences the distribute alot of the unique old type histology supplies. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Victoria Baker" To: "histonet" Sent: Thursday, August 06, 2009 1:13 PM Subject: [Histonet] metal embedding base molds > Hi- > > I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All > I'm > able to find at this point are the metal molds with the 'wings' on them. > > Does anyone know a source? With all the mergers etc it's been a little > challenging. > > Thanks in advance > > Vikki > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 6 Aug 2009 08:19:25 -1000 From: Jenee.S.Odani@hawaii.gov Subject: Re: [Histonet] Mycoplasma bovis To: "Breeden, Sara" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi there, Check out this database. It contains a contact list for the labs that are currently doing the Myco bovis IHC. http://ihc.sdstate.org/ -J Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 "Breeden, Sara" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/06/2009 08:12 AM To cc Subject [Histonet] Mycoplasma bovis My boss has thought up a new project for me so I need to know what I need to know about Mycoplasma bovis for IHC. He wants me to cost out the procedure. I normally do only TWO IHCs - CWD and BVD - so I'll be charting new mental territory with this one. Can anyone guide me on where to start figuring this out? Where do I get antibody? Is there a pre-existing protocol for the Ventana NexES? I don't believe in re-inventing the wheel if I don't need to. Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 6 Aug 2009 11:23:21 -0700 From: Maria Katleba Subject: [Histonet] (no subject) To: "histonet@lists.utsouthwestern.edu" Message-ID: <97C02552ECB11346877D3E83CF833ABD13D17C4880@SJSNT-SCMAIL03.stjoe.org> Content-Type: text/plain; charset="us-ascii" Hi All, Would anyone tell me what their lab procedure is for handling fetal demise placentas? In previous jobs sites, the histotech and/or PA would read the requisition and determine if there may be later DNA testing ordered. If yes, put in fridge and contact pathologist and/or physician. If not, place in formalin. Currently, our PA automatically places every placenta in formalin.... Kind regards, Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ------------------------------ Message: 9 Date: Thu, 6 Aug 2009 11:23:18 -0700 From: Nancy Klemme Subject: RE: [Histonet] metal embedding base molds To: Victoria Baker , histonet Message-ID: <782E3A02C2EB2347BEA6DEA69DC7AB865B4BFF12E7@sfamail.SAKURAUS.LOCAL> Content-Type: text/plain; charset="us-ascii" Dear Vikki, Sakura still carries the metal base molds that are used with Embedding Rings. They do not have the "wings" that the ones for use with the Unicassettes have. Their Sakura part numbers begin from 4121 and higher. If you use the electronic catalog on Sakura's website (www.sakuraus.com) and search by product description, just type in base molds. Then click on part # 4121 and the wingless ones will be pictured. Kind regards, Nancy Klemme, Sakura Educational Services -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Thursday, August 06, 2009 10:13 AM To: histonet Subject: [Histonet] metal embedding base molds Hi- I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm able to find at this point are the metal molds with the 'wings' on them. Does anyone know a source? With all the mergers etc it's been a little challenging. Thanks in advance Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 6 Aug 2009 12:24:53 -0700 From: "Jodie Robertson" Subject: RE: [Histonet] metal embedding base molds To: "Victoria Baker" , "histonet" Message-ID: <518CD6920AA7154193CBE5977CD880733A8AFE@psmgsrv2.PSMG.local> Content-Type: text/plain; charset="us-ascii" Try Cardinal as they are a distributor for all Tissue Tek/Sakura supplies. Jodie Robertson, HT(ASCP)QIHC Histology Day Supervisor Pathology Sciences Medical Group -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Thursday, August 06, 2009 10:13 AM To: histonet Subject: [Histonet] metal embedding base molds Hi- I'm trying to locate embedding molds that Sakura/Tissue Tek makes. All I'm able to find at this point are the metal molds with the 'wings' on them. Does anyone know a source? With all the mergers etc it's been a little challenging. Thanks in advance Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 6 Aug 2009 15:43:16 -0400 From: "DiCarlo, Margaret" Subject: [Histonet] cement in bone specimen To: Message-ID: <1B73766A27A1554CB2729B6432E81301015C76BA@KALEXMB04.KaleidaHealth.org> Content-Type: text/plain; charset="US-ASCII" Histonetters, We sliced a humerus bone on a band saw which was filled with cement from a previous surgery and my boss would like me to try to cut it on a sliding/sledge microtome. (The knife remains stationary) Is this possible to do? Presently, it is fixing and then I will decal it but I'm wondering if I'll be wasting my time and effort. I would appreciate anyone's experience with this. Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 2009 Best Places to Work Winner Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. ------------------------------ Message: 12 Date: Thu, 6 Aug 2009 14:03:11 -0600 From: Rick.Garnhart@memorialhealthsystem.com Subject: [Histonet] Re: Histonet Digest, Vol 69, Issue 6 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Does anyone out in histology have any documentation from CMS, ASCP, or NSH that breaks down the job duties or areas for what is covered under Tech and professional part of the CPT billing codes. For example is Grossing part of the a profee or tech part of the bill. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnhart@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. ------------------------------ Message: 13 Date: Thu, 6 Aug 2009 13:03:33 -0700 From: "Michelle MacVeigh-Aloni" Subject: [Histonet] What do you use for decolorizing after Hematox. To: Message-ID: <002401ca16d0$f9e4a3c0$5c237d80@DFS66DD1> Content-Type: text/plain; charset="iso-8859-1" Hi all, In my H&E staining I always used 1% acid alcohol. (Working with conc. HCl is not fun!) There is the Orth's solution available already made, but it is 3%. Do you use that? How do you use it? What do you use in your regulat H&E stain? I am using it on an old Jung AUTOSTAINER. Michelle Research Specialist USC Keck School of Medicine ------------------------------ Message: 14 Date: Thu, 6 Aug 2009 16:21:41 -0400 From: Jack Ratliff Subject: RE: [Histonet] cement in bone specimen To: , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Peggy, If the cement you mention is or similar to PMMA, then you will not be able to cut thin sections using the sliding/sledge microtome. If I was you, I would not decal it at all as it will only make the bone less dense than the cement and will definitely waste your time if you embed it in paraffin to try and cut later. Your best and only chance at success here is to embed undemineralized in resin and cut thick or ground sections that can be polished to a desired thickness. If you would like to discuss this further, please give me a call at 317-281-1975. Best, Jack Ratliff > Date: Thu, 6 Aug 2009 15:43:16 -0400 > From: MDiCarlo@KaleidaHealth.Org > To: histonet@pathology.swmed.edu > CC: > Subject: [Histonet] cement in bone specimen > > Histonetters, > > > > We sliced a humerus bone on a band saw which was filled with cement from > a previous surgery and my boss would like me to try to cut it on a > sliding/sledge microtome. (The knife remains stationary) Is this > possible to do? Presently, it is fixing and then I will decal it but > I'm wondering if I'll be wasting my time and effort. > > > > I would appreciate anyone's experience with this. > > > > Thank you. > > > > Peggy DiCarlo HT (ASCP) > > Orthopedic Bone Lab > > Buffalo General Hospital > > Buffalo, NY 14203 > > 716-859-1293 > > > > > > 2009 Best Places to Work Winner > Visit our careers page at www.kaleidahealth.org/careers > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 06 Aug 2009 16:53:16 -0400 From: mtitford@aol.com Subject: [Histonet] Fetal demise placenta To: Histonet@pathology.swmed.edu Message-ID: <8CBE4EF0E4C2479-93C-E73@webmail-me19.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Maria asks about fetal demise placenta: We gross in all fetal demise placenta (usually four blocks).If the clinicians desire genetics, they take?samples from the fetus and the placenta up on the floor. (There is only a three day window for growing fibroblasts). The fetus gets a gross only treatment with weight, measurements and a photograph. If the clinicians or family desire an autopsy, whatever the gestational age, we have the parents sign an autopsy consent, as if for an adult. Regards Michael Titford USA Pathology Mobile AL ------------------------------ Message: 16 Date: Fri, 7 Aug 2009 09:05:35 +0300 From: Vesterinen Tiina Subject: [Histonet] HPGD, PAFa and frizzled-4 stainings on Ventana Benchmark To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <2C0982D85C07A34598393F0B2B6F9675413886824E@vanhusmbx06.hus.fi> Content-Type: text/plain; charset="iso-8859-1" Hi everyone, Has anyone stained formalin fixed, paraffin embedded slices with HPGD (Sigma), PAF acetylhydrolase (Cayman Chemical) or frizzled-4 (Santa Cruz) on Ventana Discovery, Benchmark or similar autostainer? I would appreciate all advices related to staining protocols because I4ve quite small amounts of antibodies. Regards, Tiina Vesterinen Tiina Vesterinen Project Researcher Institute for Molecular Medicine Finland FIMM Helsinki, Finland ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 69, Issue 7 *************************************** From jkiernan <@t> uwo.ca Wed Aug 26 12:04:45 2009 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Aug 26 12:04:49 2009 Subject: [Histonet] bubbles under coverslip In-Reply-To: <883841429.17431251227574555.JavaMail.osg@osgjas02.cns.ufl.edu> References: <883841429.17431251227574555.JavaMail.osg@osgjas02.cns.ufl.edu> Message-ID: The cause of your bubble trouble is replacing xylene with a liquid whose identity is unknown and whose physical properties are obviously different. For an important application such as research or diagnosis it is not scientifically or ethically justifiable to use unknown chemicals. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "FU,DONGTAO" Date: Tuesday, August 25, 2009 15:13 Subject: [Histonet] bubbles under coverslip To: histonet@lists.utsouthwestern.edu > Hi, all > > Recently we met a problem of bubbles under coverslip > after we > changed to Xylene Substitute(from Shandon) instead of Xylene. > The > bubbles immediately appear after coverslipping. At very > beginning, > we thought it was due to mounting medium. So we changed to > Shandon > Xylene Substitue Mountant, but it did not help. Right now we > have > to transfer slides from Xylene Substitute to xylene, then > coverslip them. We hate the extra xylene step! > > Does anyone know what cause the bubbles? And how to avoid > it? > Any suggestions will be appreciated. > > Ann Dongtao Fu > Dept. of Pathology > University of Flodrida > Gainesville, FL 32610 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pat.Bell <@t> ucdenver.edu Wed Aug 26 12:20:22 2009 From: Pat.Bell <@t> ucdenver.edu (Bell, Pat) Date: Wed Aug 26 12:23:09 2009 Subject: [Histonet] Sakura Products In-Reply-To: References: , Message-ID: <64DB27005E2FD3439E88502D7A5C91218A7BA9D1D8@CORTEZ.ucdenver.pvt> Just don't buy the slide printer. Pat Bell, HT(ASCP) University of Colorado Denver ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley [azdudley@hotmail.com] Sent: Wednesday, August 26, 2009 10:24 AM To: emily@pathlabsolutions.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura Products emily, we have a sakura processor, embedding center, an accu-cut SRM microtome and a coverslipper. they are all great. you can't go wrong with sakura. anita > Date: Tue, 25 Aug 2009 08:54:22 -0400 > From: emily@pathlabsolutions.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Sakura Products > > Hi All: > > I'm looking to purchase equipment for a new lab and would like the opinion > of anyone who has worked with various Sakura products before... good or bad. > I would be looking to buy the following products: > > Tissue-Tek TEC 5 Embedding Station > Accu-Cut SRM 200 Microtome > Cyto-Tek Cytocentrifuge > > Also if anyone has experience with prostate and GI bx processed in the > Tissue-Tek Paraform Sectional Cassette System, I'd appreciate input on that > too. > > Thanks! > > -- > Emily Butte, M.S., HTL (ASCP) > Path Lab Solutions Inc. > (701) 371-7515 > emily@pathlabsolutions.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JPEPOON <@t> PARTNERS.ORG Wed Aug 26 12:24:08 2009 From: JPEPOON <@t> PARTNERS.ORG (Pepoon, James R.) Date: Wed Aug 26 12:24:13 2009 Subject: [Histonet] Clinical Supervisor, IHC Employment Opportunity Message-ID: Hello, We are currently searching for a Clinical Supervisor for our growing Immunopathology Laboratory within the Department of Pathology at Brigham and Women's Hospital. If you are interested, the posting is available online at www.brighamandwomens.org. Clinical Supervisor, Immunopathology. Job ID: 2193186. You may direct questions and/or send CV's to: jpepoon@partners.org Jim > James R. Pepoon, HT(ASCP)QIHC, CLCP > Technical Director > Department of Pathology > Brigham and Women's Hospital > 75 Francis Street > Boston, Massachusetts 02115 > > > > The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From JWeems <@t> sjha.org Wed Aug 26 12:25:17 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Aug 26 12:25:24 2009 Subject: [Histonet] Sakura Products In-Reply-To: <64DB27005E2FD3439E88502D7A5C91218A7BA9D1D8@CORTEZ.ucdenver.pvt> References: , <64DB27005E2FD3439E88502D7A5C91218A7BA9D1D8@CORTEZ.ucdenver.pvt> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA577EC7E@ITSSSXM01V6.one.ads.che.org> Ours works very well!! Both cassette and slide printer are interfaces with LIS. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Pat Sent: Wednesday, August 26, 2009 13:20 To: anita dudley; emily@pathlabsolutions.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura Products Just don't buy the slide printer. Pat Bell, HT(ASCP) University of Colorado Denver ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley [azdudley@hotmail.com] Sent: Wednesday, August 26, 2009 10:24 AM To: emily@pathlabsolutions.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura Products emily, we have a sakura processor, embedding center, an accu-cut SRM microtome and a coverslipper. they are all great. you can't go wrong with sakura. anita > Date: Tue, 25 Aug 2009 08:54:22 -0400 > From: emily@pathlabsolutions.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Sakura Products > > Hi All: > > I'm looking to purchase equipment for a new lab and would like the > opinion of anyone who has worked with various Sakura products before... good or bad. > I would be looking to buy the following products: > > Tissue-Tek TEC 5 Embedding Station > Accu-Cut SRM 200 Microtome > Cyto-Tek Cytocentrifuge > > Also if anyone has experience with prostate and GI bx processed in the > Tissue-Tek Paraform Sectional Cassette System, I'd appreciate input on > that too. > > Thanks! > > -- > Emily Butte, M.S., HTL (ASCP) > Path Lab Solutions Inc. > (701) 371-7515 > emily@pathlabsolutions.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail(r) is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en- US:WM_HYGN_faster:082009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From JWeems <@t> sjha.org Wed Aug 26 12:27:50 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Aug 26 12:27:57 2009 Subject: [Histonet] Sakura Products In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA577EC7E@ITSSSXM01V6.one.ads.che.org> References: , <64DB27005E2FD3439E88502D7A5C91218A7BA9D1D8@CORTEZ.ucdenver.pvt> <5D64396A0D4A5346BEBC759022AAEAA577EC7E@ITSSSXM01V6.one.ads.che.org> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA577EC81@ITSSSXM01V6.one.ads.che.org> Make that interfaced! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, August 26, 2009 13:25 To: Bell, Pat; anita dudley; emily@pathlabsolutions.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura Products Ours works very well!! Both cassette and slide printer are interfaces with LIS. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Pat Sent: Wednesday, August 26, 2009 13:20 To: anita dudley; emily@pathlabsolutions.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura Products Just don't buy the slide printer. Pat Bell, HT(ASCP) University of Colorado Denver ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley [azdudley@hotmail.com] Sent: Wednesday, August 26, 2009 10:24 AM To: emily@pathlabsolutions.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura Products emily, we have a sakura processor, embedding center, an accu-cut SRM microtome and a coverslipper. they are all great. you can't go wrong with sakura. anita > Date: Tue, 25 Aug 2009 08:54:22 -0400 > From: emily@pathlabsolutions.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Sakura Products > > Hi All: > > I'm looking to purchase equipment for a new lab and would like the > opinion of anyone who has worked with various Sakura products before... good or bad. > I would be looking to buy the following products: > > Tissue-Tek TEC 5 Embedding Station > Accu-Cut SRM 200 Microtome > Cyto-Tek Cytocentrifuge > > Also if anyone has experience with prostate and GI bx processed in the > Tissue-Tek Paraform Sectional Cassette System, I'd appreciate input on > that too. > > Thanks! > > -- > Emily Butte, M.S., HTL (ASCP) > Path Lab Solutions Inc. > (701) 371-7515 > emily@pathlabsolutions.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail(r) is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en- US:WM_HYGN_faster:082009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From anh2006 <@t> med.cornell.edu Wed Aug 26 13:05:41 2009 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Wed Aug 26 13:05:47 2009 Subject: [Histonet] Double HRP immunostains Message-ID: I do double HRP immunostains. Works well using DAB/AEC and True Blue ... but what are your favorite chromagens to use? Would love some feedback for inspiration. Andrea -- From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed Aug 26 14:25:15 2009 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed Aug 26 14:25:20 2009 Subject: [Histonet] Luxol Fast Blue with IHC In-Reply-To: References: Message-ID: <5F3F860CFE0F4741B1D87A88A58FAE9A63F11E@mailbe01.mc.vanderbilt.edu> Hi Nicole, I do LFB with IHC on FFPE human brain but there are a few differences in our protocols. I have stained LFB with Neurofilament and with S-100 but never with Ki-67. I also use DAB as my chromagen and differentiate the LFB with dilute Lithium Carbonate. I'm not familiar with the VIP chromagen you mention, so I'm just guessing here (Perhaps somebody on histonet can fill in that blank for us). If VIP chromagen is insoluble in alcohol/xylene, you will likely be fine. I do reduce my staining time in LFB a little so that it doesn't overpower the IHC. You may want to try an LBF protocol with lithium carbonate. It won't hurt IHC at all. One other hint: LFB before IHC doesn't work for me. When I perform antigen retrieval, all of my LFB is lost. If you haven't tried it yet with your current reagents, that would be my first step. If you KNOW that VIP will be removed, I'd try to "borrow" some DAB from a neighbor. Good luck and don't hesitate to shoot me an email if you have any additional questions. Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: Patten, Nicole (NIH/NIAAA) [F] [mailto:pattennj@mail.nih.gov] Sent: Wednesday, August 26, 2009 10:03 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Luxol Fast Blue with IHC Hello- Has anyone tried to counterstain an IHC stain with Luxol Fast Blue? I am worried that the LFB destain will remove some of the IHC. I use the VIP solution from Vector as the chromogen for IHC instead of DAB and I destain Luxol Fast Blue with Hydroquinone/Sodium Sulfite (in addition to the ethanol steps). I'm staining for Ki-67 and I'm doing this on FFPE human brain sections. I haven't found anything in the Histonet archives on this topic. Any advice would be greatly appreciated. Thanks in advance! -Nicole, Post-bacccalaureate Fellow, NIH From bamoe <@t> gundluth.org Wed Aug 26 15:38:18 2009 From: bamoe <@t> gundluth.org (bamoe@gundluth.org) Date: Wed Aug 26 15:38:36 2009 Subject: [Histonet] Organizing of cassettes for processing Message-ID: Hi all - For those that do batch overnight processing, how do you organize the cassettes? Currently we have 2 path assistants that gross throughout the day, and each puts their cassettes as they are grossed into a bucket of formalin. At the end of the day a histotech drains the formalin off, rinses the cassettes in water, then manually puts the cassettes into order according to our worklist, with rush cases being put up front. The baskets are then loaded onto the tissue processor (Sakura VIP5 and VIP6). We are wondering if there are some other ideas of how to streamline this process. One thought was to have the cassettes loaded/organized into the tissue processing basket as they are grossed, but have a concern about formalin exposure while doing this. Any thoughts would be greatly appreciated. Barb Moe Gundersen Lutheran Medical Center La Crosse WI From trathborne <@t> somerset-healthcare.com Wed Aug 26 15:48:59 2009 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Aug 26 15:49:05 2009 Subject: [Histonet] Organizing of cassettes for processing In-Reply-To: Message-ID: We have 2 grossing stations, end each person places their cassettes directly into the VIP basket. They go in numerically for the most part. The basket is kept in a rectangular container with formalin and covered/partially covered during the day. (You can get a container of this sort in your local Wal-Mart or other similar store.) Then, the histotechs embed by cassette color (GI's one color, breast cores another, etc.), and by case number. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of bamoe@gundluth.org Sent: Wednesday, August 26, 2009 4:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Organizing of cassettes for processing Hi all - For those that do batch overnight processing, how do you organize the cassettes? Currently we have 2 path assistants that gross throughout the day, and each puts their cassettes as they are grossed into a bucket of formalin. At the end of the day a histotech drains the formalin off, rinses the cassettes in water, then manually puts the cassettes into order according to our worklist, with rush cases being put up front. The baskets are then loaded onto the tissue processor (Sakura VIP5 and VIP6). We are wondering if there are some other ideas of how to streamline this process. One thought was to have the cassettes loaded/organized into the tissue processing basket as they are grossed, but have a concern about formalin exposure while doing this. Any thoughts would be greatly appreciated. Barb Moe Gundersen Lutheran Medical Center La Crosse WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. -------------------------------------------------------------- Somerset Medical Center is the recipient of the 2009 Orthopedic Surgery Excellence Award(tm) from HealthGrades, the nation's leading health care ratings company. Visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for news, event listings, health information and more. Join the Discussion: Facebook: www.somersetmedicalcenter.com/fb Twitter: www.twitter.com/SomersetMedCtr From RCazares <@t> schosp.org Wed Aug 26 16:21:38 2009 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Wed Aug 26 16:21:43 2009 Subject: [SPAM-HC] - [Histonet] Organizing of cassettes for processing - Email found in subject In-Reply-To: References: Message-ID: <572D1F45B44B3D4096D554B4CB40639C4F231DB3@EXCHCCRMB.schosp.org> Hi Barb, We have metal sleeves in which 18 cassettes fit standing front to back, and a metal box with lid which we fill with formalin. This box holds 6 sleeves and as cases are grossed, the cassettes are picked up in order (it doesn't take any effort to do this at the time of grossing) and placed in the sleeves. We have two metal box/containers in which we separate our routines and our biopsies (we use a short program for our biopsies). At the end of the day we print out hard copies of all the cases that will be cut the next morning, separating the routines from the biopsies so there are 2 lists, and then we go cassette by cassete and check off on the list to assure that every cassette is accounted for. This system works great for us and it helps to find errors before they go any further. We use colored dots on the cases that are biopsies so when its time to print out our log sheets we can do so from the requisitions or working log sheet, and if done right, every case and cassette should match up. I believe Leica now carries these sleeves and metal box containers, I strongly urge you to look into them, they keep things so organized and we LOVE them!! I can get you ordering information, just let me know. Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of bamoe@gundluth.org Sent: Wednesday, August 26, 2009 3:38 PM To: histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - [Histonet] Organizing of cassettes for processing - Email found in subject Hi all - For those that do batch overnight processing, how do you organize the cassettes? Currently we have 2 path assistants that gross throughout the day, and each puts their cassettes as they are grossed into a bucket of formalin. At the end of the day a histotech drains the formalin off, rinses the cassettes in water, then manually puts the cassettes into order according to our worklist, with rush cases being put up front. The baskets are then loaded onto the tissue processor (Sakura VIP5 and VIP6). We are wondering if there are some other ideas of how to streamline this process. One thought was to have the cassettes loaded/organized into the tissue processing basket as they are grossed, but have a concern about formalin exposure while doing this. Any thoughts would be greatly appreciated. Barb Moe Gundersen Lutheran Medical Center La Crosse WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From vapatpxs <@t> yahoo.com Wed Aug 26 16:40:38 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Wed Aug 26 16:40:41 2009 Subject: [Histonet] Organizing of cassettes for processing In-Reply-To: Message-ID: <543615.15789.qm@web46102.mail.sp1.yahoo.com> When I batted clean up and loaded the processors, the residents grossed in the specimens and placed them in a basket in a container of formalin with a lid. The grossing was done and the containers were kept on the downdraft table. When it came time to load the processors I put the cassettes in numerical order and in separate baskets (smalls like GI biopsies and needle biopsies went together in one basket, fatty samples like breast tissue in another basket, etc). I did this on the downdraft table and placed the cassettes in numerical order. This helped us to find out if samples were mislabelled (2 cassettes with the same number) or missing (samples labelled A-F and D was missing). This saved our bacon many times since I would make notes or ask the resident where the sample was or which was which. It also made it easier for the embedders since I placed the notes regarding the missing cassettes by the embedding stations. Plus they could embed the block in numeric order so the slides that needed to get out first were cut and stained first. Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Wed, 8/26/09, bamoe@gundluth.org wrote: > From: bamoe@gundluth.org > Subject: [Histonet] Organizing of cassettes for processing > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, August 26, 2009, 8:38 PM > > Hi all - > > For those that do batch overnight processing, how do you > organize the > cassettes? > > Currently we have 2 path assistants that gross throughout > the day, and each > puts their cassettes as they are grossed into a bucket of > formalin.? At the > end of the day a histotech drains the formalin off, rinses > the cassettes in > water, then manually puts the cassettes into order > according to our > worklist, with rush cases being put up front.? The > baskets are then loaded > onto the tissue processor (Sakura VIP5 and VIP6). > > We are wondering if there are some other ideas of how to > streamline this > process.? One thought was to have the cassettes > loaded/organized into the > tissue processing basket as they are grossed, but have a > concern about > formalin exposure while doing this. > > Any thoughts would be greatly appreciated. > > Barb Moe > Gundersen Lutheran Medical Center > La Crosse WI > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dhill32 <@t> comcast.net Wed Aug 26 16:57:33 2009 From: dhill32 <@t> comcast.net (David Hill) Date: Wed Aug 26 16:57:05 2009 Subject: [Histonet] IHC'S on Cellient blocks Message-ID: <25E87B6B295A462FACFB2B86D5183B2F@DavidPC> Is anyone out there performing IHC'S on the Benchmark XT's with Cellient block sections successfully? If so would you please send me your protocol. I'm currently trying them out at my lab with little success. We are deparaffinizing, rehydrating and then fixing for 10 minutes in 10% NBF before placing on the XT's and not getting good results. Thanks in advance. David Hill B.S. CT(ASCP) HTL, QIHC Assistant Histology Supervisor Trumbull Laboratories Memphis, TN From Maria.Katleba <@t> stjoe.org Wed Aug 26 18:22:43 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Wed Aug 26 18:22:51 2009 Subject: [Histonet] New Grossing station for sale Message-ID: <97C02552ECB11346877D3E83CF833ABD13D5353EDC@SJSNT-SCMAIL03.stjoe.org> Hi All, Well, I was given the green light: It's a Shannon Grosslab Senior Workstation. It was delivered September 2004...stored ever since due to the fact it was too big to instal in the small space. (Someone doesn't know how to measure!) The item purchase price was $15,484.86 and has a current book value of $8,000.49.... you know, depreciation! It has never been used. In fact, in storage with the plastic wrap still on. Let me know if you are interested. We are trying to get rid of it since we had to buy a smaller one to better fit our lab. Call me or -email me for the details! Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From gareth.davis <@t> hotmail.com Wed Aug 26 19:04:59 2009 From: gareth.davis <@t> hotmail.com (Gareth Blaeuer Davis) Date: Wed Aug 26 19:06:31 2009 Subject: [Histonet] Best IHC stainer Message-ID: The lab I work in has been doing demos on IHC stainers Ventana Benchmark XT and Dako's Autostainer Plus. We could really use feed back from current users of both. We are having a hard time deciding between the two, so any input would be great. What are the Pros and Cons with both. Thanks, Gareth Blaeuer Davis, B.S., HT _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009 From JWeems <@t> sjha.org Wed Aug 26 20:57:04 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Aug 26 21:01:24 2009 Subject: [Histonet] Best IHC stainer References: Message-ID: <5D64396A0D4A5346BEBC759022AAEAA53C2FD9@ITSSSXM01V6.one.ads.che.org> They are both good instruments. From my experience pricing is high for the Ventana reagents and there seems to be an increase in DAKO pricing from what I see on the Net. Have you looked at the Leica Bond? We found that to be a good fit for us. Started out to add it to our DAKO line, but ended up changing completely. It also does ISH, and is a continuous feed - up to 10 slides at a time. Good luck, Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gareth Blaeuer Davis Sent: Wed 8/26/2009 8:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Best IHC stainer The lab I work in has been doing demos on IHC stainers Ventana Benchmark XT and Dako's Autostainer Plus. We could really use feed back from current users of both. We are having a hard time deciding between the two, so any input would be great. What are the Pros and Cons with both. Thanks, Gareth Blaeuer Davis, B.S., HT _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From annigyg <@t> gmail.com Thu Aug 27 05:23:59 2009 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Aug 27 05:24:04 2009 Subject: [Histonet] DIF tissue in GLUT Message-ID: Dear John and other knowledgeable Histonetters and Gurus of the trade.... Renal bx tissue core has been placed in Gluteraldehyde/Trumps EM fixative by mistake (we all make mistakes) - we now need to do DIF Does anyone out there have a method for this? -- Anne van Binsbergen (Hope) Abu Dhabi UAE From gvdobbin <@t> ihis.org Thu Aug 27 06:45:24 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Aug 27 06:46:12 2009 Subject: [Histonet] Best IHC stainer Message-ID: I echo Joyce's comments! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Weems, Joyce" 8/26/2009 10:57 PM >>> They are both good instruments. From my experience pricing is high for the Ventana reagents and there seems to be an increase in DAKO pricing from what I see on the Net. Have you looked at the Leica Bond? We found that to be a good fit for us. Started out to add it to our DAKO line, but ended up changing completely. It also does ISH, and is a continuous feed - up to 10 slides at a time. Good luck, Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gareth Blaeuer Davis Sent: Wed 8/26/2009 8:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Best IHC stainer The lab I work in has been doing demos on IHC stainers Ventana Benchmark XT and Dako's Autostainer Plus. We could really use feed back from current users of both. We are having a hard time deciding between the two, so any input would be great. What are the Pros and Cons with both. Thanks, Gareth Blaeuer Davis, B.S., HT _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From c.m.vanderloos <@t> amc.uva.nl Thu Aug 27 07:38:31 2009 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu Aug 27 07:38:50 2009 Subject: [Histonet] RE: Double HRP immunostains Message-ID: <806f6cf44b66727b.4a969a67@amc.uva.nl> Andrea,Go for: Vector Blue (Vector Labs) or Permanent Blue (Diagnostic Biosystems) for AP activityVector Red or Permanent Red, also for AP activity. These two AP immunostaining methods can be combined in a sequential double staining method with a HIER step (10 min, 98C) in between for removing the immunoreagents. Both red or blue reaction products survive the HIER step without damage (see JOH 31:119-127). Cheers, ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Wed, 26 Aug 2009 14:05:41 -0400 From: Andrea Hooper Subject: [Histonet] Double HRP immunostains To: Histonet I do double HRP immunostains. Works well using DAB/AEC and True Blue ... but what are your favorite chromagens to use? Would love some feedback for inspiration. Andrea -- From gregor <@t> arlt-digital.de Thu Aug 27 08:04:44 2009 From: gregor <@t> arlt-digital.de (gregor@arlt-digital.de) Date: Thu Aug 27 08:04:56 2009 Subject: [Histonet] Sakura Printers Message-ID: <43270180.594.1251378284791.JavaMail.open-xchange@oxltgw02.schlund.de> Hi Joyce, Hi Pat, >From my experience the printers are working very well. There could be issues if any of the adjustments are of or the slides or cassettes that are used not fit to the system. What kinds of problems do you having? What makes you unhappy with the printer? @Joyce: I would like to know what kind of LIS system do you using. Do you have the printers connected directly to a computer or do you using a network switch as an interface to the printers. Thanks Gregor From rjbuesa <@t> yahoo.com Thu Aug 27 08:05:15 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 27 08:05:21 2009 Subject: [Histonet] Best IHC stainer In-Reply-To: Message-ID: <745139.3257.qm@web65713.mail.ac4.yahoo.com> I prefer the DAKO. Check, besides the instrument itself, the reagents COST and how "open" one system is compared with the other. Ren? J. --- On Wed, 8/26/09, Gareth Blaeuer Davis wrote: From: Gareth Blaeuer Davis Subject: [Histonet] Best IHC stainer To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 26, 2009, 8:04 PM The lab I work in has been doing demos on IHC stainers Ventana Benchmark XT and Dako's Autostainer Plus.? We could really use feed back from current users of both.? We are having a hard time deciding between the two, so any input would be great. What are the Pros and Cons with both. Thanks, Gareth Blaeuer Davis, B.S., HT _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Thu Aug 27 08:41:02 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Aug 27 08:41:11 2009 Subject: [Histonet] Confirmation of reports Message-ID: <65365F35C0F2EF4D846EC3CA73E49C438E0A2E2030@HPEMX3.HealthPartners.int> We use Copath for Histology LIS and Epic as our Hospital and clinic based system. In Epic, you can retrieve who reviewed your results by looking at the result in Chart Review. Look below the results, it lists who and when. There is also an electronic trail that Epic can produce if needed. Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From cbrya <@t> lexclin.com Thu Aug 27 08:55:49 2009 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Thu Aug 27 08:55:54 2009 Subject: [Histonet] Organizing of cassettes for processing In-Reply-To: References: Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF172B39CA@EXCHANGESB> We load the cassettes into the tissue processing basket as they are grossed in. They stay in a container of formalin until they are loaded on the tissue processor. What concern about formalin exposure do you have with this method? The gross hood is on while they are doing this. We also monitor our formalin exposure annually and I have the path assistant wear the formalin badge while she performs this task. Our readings have always been well below the acceptable range. If there is a better method I may be interested in it also. Thanks, Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of bamoe@gundluth.org Sent: Wednesday, August 26, 2009 4:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Organizing of cassettes for processing Hi all - For those that do batch overnight processing, how do you organize the cassettes? Currently we have 2 path assistants that gross throughout the day, and each puts their cassettes as they are grossed into a bucket of formalin. At the end of the day a histotech drains the formalin off, rinses the cassettes in water, then manually puts the cassettes into order according to our worklist, with rush cases being put up front. The baskets are then loaded onto the tissue processor (Sakura VIP5 and VIP6). We are wondering if there are some other ideas of how to streamline this process. One thought was to have the cassettes loaded/organized into the tissue processing basket as they are grossed, but have a concern about formalin exposure while doing this. Any thoughts would be greatly appreciated. Barb Moe Gundersen Lutheran Medical Center La Crosse WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From godsgalnow <@t> aol.com Thu Aug 27 10:05:35 2009 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Aug 27 10:06:05 2009 Subject: [Histonet] Best IHC stainer In-Reply-To: References: Message-ID: <8CBF53EF6AD28B5-14F0-36B5A@webmail-d004.sysops.aol.com> Ventana reagents eem to be a little pricey, but if you want a system that you can load and walk away from, it is the way to go.? DAKO is an easy enough to use instrument and is an open system, but they are going to a price per slide kind of thing, if I remmeber correctly. I prefer the IP from Biocare, as is is an open system and a continuous load instrument.? And it mixes the chromagen online.... -----Original Message----- From: Gareth Blaeuer Davis To: histonet@lists.utsouthwestern.edu Sent: Wed, Aug 26, 2009 8:04 pm Subject: [Histonet] Best IHC stainer he lab I work in has been doing demos on IHC stainers Ventana Benchmark XT and ako's Autostainer Plus. We could really use feed back from current users of both. We are having a hard time deciding between the two, o any input would be great. What are the Pros and Cons with both. Thanks, Gareth Blaeuer Davis, B.S., HT _________________________________________________________________ otmail? is up to 70% faster. Now good news travels really fast. ttp://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009_______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Aug 27 10:19:43 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Aug 27 10:19:53 2009 Subject: [Histonet] RE: Sakura Printers In-Reply-To: <43270180.594.1251378284791.JavaMail.open-xchange@oxltgw02.schlund.de> References: <43270180.594.1251378284791.JavaMail.open-xchange@oxltgw02.schlund.de> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA577EE29@ITSSSXM01V6.one.ads.che.org> We have GE Ultra (formerly Triple G) LIS. It is interfaced directly into the printers. j ________________________________ From: gregor@arlt-digital.de [mailto:gregor@arlt-digital.de] Sent: Thursday, August 27, 2009 09:05 To: Pat.Bell@ucdenver.edu; Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: Sakura Printers Hi Joyce, Hi Pat, >From my experience the printers are working very well. There could be issues if any of the adjustments are of or the slides or cassettes that are used not fit to the system. What kinds of problems do you having? What makes you unhappy with the printer? @Joyce: I would like to know what kind of LIS system do you using. Do you have the printers connected directly to a computer or do you using a network switch as an interface to the printers. Thanks Gregor Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Jackie.O'Connor <@t> abbott.com Thu Aug 27 10:22:32 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Aug 27 10:22:59 2009 Subject: [Histonet] Best IHC stainer In-Reply-To: <8CBF53EF6AD28B5-14F0-36B5A@webmail-d004.sysops.aol.com> Message-ID: Agreed. Intellipath from Biocare. Load it and walk away - and it has a voice notification to tell you the run is finished. You can load it in the PM, and take slides off in the AM. Love it. Has a chilling station for AP chromogen, too. Jacke O' godsgalnow@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 08/27/2009 10:05 AM To gareth.davis@hotmail.com, histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] Best IHC stainer Ventana reagents eem to be a little pricey, but if you want a system that you can load and walk away from, it is the way to go. DAKO is an easy enough to use instrument and is an open system, but they are going to a price per slide kind of thing, if I remmeber correctly. I prefer the IP from Biocare, as is is an open system and a continuous load instrument. And it mixes the chromagen online.... -----Original Message----- From: Gareth Blaeuer Davis To: histonet@lists.utsouthwestern.edu Sent: Wed, Aug 26, 2009 8:04 pm Subject: [Histonet] Best IHC stainer he lab I work in has been doing demos on IHC stainers Ventana Benchmark XT and ako's Autostainer Plus. We could really use feed back from current users of both. We are having a hard time deciding between the two, o any input would be great. What are the Pros and Cons with both. Thanks, Gareth Blaeuer Davis, B.S., HT _________________________________________________________________ otmail? is up to 70% faster. Now good news travels really fast. ttp://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009_______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dunatrsd <@t> sbcglobal.net Thu Aug 27 10:25:26 2009 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Thu Aug 27 10:25:32 2009 Subject: [Histonet] Best IHC stainer In-Reply-To: <8CBF53EF6AD28B5-14F0-36B5A@webmail-d004.sysops.aol.com> References: <8CBF53EF6AD28B5-14F0-36B5A@webmail-d004.sysops.aol.com> Message-ID: <20620.68069.qm@web83910.mail.sp1.yahoo.com> Having worked with Dako autostainer for years and currently using Vantana Discovery XT and Leica Bond stainer's, my preference would be the Bond. You have the capability of the Ventana stainer, and being able to unlock the software that will allow you to use it as any other stainer on the market today. Bond also has a favorable per slide cost analysis. If you need any additional info, I would be happy to provide as much as I am can. Thanks Dusko Trajkovic Pfizer Inc La Jolla 858-638-6202 ________________________________ From: "godsgalnow@aol.com" To: gareth.davis@hotmail.com; histonet@lists.utsouthwestern.edu Sent: Thursday, August 27, 2009 8:05:35 AM Subject: Re: [Histonet] Best IHC stainer Ventana reagents eem to be a little pricey, but if you want a system that you can load and walk away from, it is the way to go.? DAKO is an easy enough to use instrument and is an open system, but they are going to a price per slide kind of thing, if I remmeber correctly. I prefer the IP from Biocare, as is is an open system and a continuous load instrument.? And it mixes the chromagen online.... -----Original Message----- From: Gareth Blaeuer Davis To: histonet@lists.utsouthwestern.edu Sent: Wed, Aug 26, 2009 8:04 pm Subject: [Histonet] Best IHC stainer he lab I work in has been doing demos on IHC stainers Ventana Benchmark XT and ako's Autostainer Plus.? We could really use feed back from current users of both.? We are having a hard time deciding between the two, o any input would be great. What are the Pros and Cons with both. Thanks, Gareth Blaeuer Davis, B.S., HT _________________________________________________________________ otmail? is up to 70% faster. Now good news travels really fast. ttp://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009_______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Aug 27 10:25:56 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Aug 27 10:26:04 2009 Subject: AW: [Histonet] Organizing of cassettes for processing In-Reply-To: References: Message-ID: <9B4E9B3310D843D999848C2305CA8022@dielangs.at> We seperate biopsies and grossed tissue before grossing. They get into differently coloured cassettes, and different VIPs. The cassettes of the grossed tissue are put directly into the VIP baskets - short time dry, then put into a container with formalin. While grossing a report is written. So the order of the cassettes is mostly the same as the numbers on the report. Urgent cases have an extra colour. After embedding, the cassettes and the report are checked. Urgent cases are embedded first, then biopsies, grossed tissue is sorted according to the report not numbers. With slide-delivering we check sildes and report again. So mislabelled or missing cassettes and slides are identified. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von bamoe@gundluth.org Gesendet: Mittwoch, 26. August 2009 22:38 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Organizing of cassettes for processing Hi all - For those that do batch overnight processing, how do you organize the cassettes? Currently we have 2 path assistants that gross throughout the day, and each puts their cassettes as they are grossed into a bucket of formalin. At the end of the day a histotech drains the formalin off, rinses the cassettes in water, then manually puts the cassettes into order according to our worklist, with rush cases being put up front. The baskets are then loaded onto the tissue processor (Sakura VIP5 and VIP6). We are wondering if there are some other ideas of how to streamline this process. One thought was to have the cassettes loaded/organized into the tissue processing basket as they are grossed, but have a concern about formalin exposure while doing this. Any thoughts would be greatly appreciated. Barb Moe Gundersen Lutheran Medical Center La Crosse WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Aug 27 10:32:25 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Aug 27 10:32:30 2009 Subject: AW: [Histonet] DIF tissue in GLUT In-Reply-To: References: Message-ID: <48EB5B08DAFB432593BB2B4AC87EA3AB@dielangs.at> Anne, we do DIF on formalin fixed renal biopsies routinly. To get good results we perform Protease digestion before DIF. I don't have the protocol here, but we use Proteinase K from Dako at RT for 3-4 min, and the antibodies are between 1:20 and 1:40 f?r 30 min. I don't know, if that would help with glutaraldehyde-fixed specimen, but worth trying. Gudrun Lang Akh Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Anne van Binsbergen Gesendet: Donnerstag, 27. August 2009 12:24 An: John Kiernan; histonet@lists.utsouthwestern.edu Betreff: [Histonet] DIF tissue in GLUT Dear John and other knowledgeable Histonetters and Gurus of the trade.... Renal bx tissue core has been placed in Gluteraldehyde/Trumps EM fixative by mistake (we all make mistakes) - we now need to do DIF Does anyone out there have a method for this? -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dchihc <@t> yahoo.com Thu Aug 27 11:22:22 2009 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Thu Aug 27 11:22:25 2009 Subject: [Histonet] Michael LaFriniere Message-ID: <618235.49341.qm@web43504.mail.sp1.yahoo.com> ?If anyone knows how to contact Michael, please email me. Thanks! Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From RCazares <@t> schosp.org Thu Aug 27 11:36:50 2009 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Thu Aug 27 11:36:56 2009 Subject: [SPAM-HC] - [Histonet] Organizing of cassettes for processing - Email found in subject References: Message-ID: <572D1F45B44B3D4096D554B4CB40639C4F231F14@EXCHCCRMB.schosp.org> I gave out misinformation which I want to rectify. The metal sleeves and holding tray is sold by McCormick Scientific which is a part of Leica, but they have their own ordering website: http://www.mccormickscientific.com/cassettemangement.asp?PCID=853 The above link should open to the item itself. The ordering information is- # MC 100 Formalin Holding tank # MC 101 Formalin Tank Lid Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -----Original Message----- From: Cazares, Ruth Sent: Wednesday, August 26, 2009 4:22 PM To: 'bamoe@gundluth.org'; histonet@lists.utsouthwestern.edu Subject: RE: [SPAM-HC] - [Histonet] Organizing of cassettes for processing - Email found in subject Hi Barb, We have metal sleeves in which 18 cassettes fit standing front to back, and a metal box with lid which we fill with formalin. This box holds 6 sleeves and as cases are grossed, the cassettes are picked up in order (it doesn't take any effort to do this at the time of grossing) and placed in the sleeves. We have two metal box/containers in which we separate our routines and our biopsies (we use a short program for our biopsies). At the end of the day we print out hard copies of all the cases that will be cut the next morning, separating the routines from the biopsies so there are 2 lists, and then we go cassette by cassete and check off on the list to assure that every cassette is accounted for. This system works great for us and it helps to find errors before they go any further. We use colored dots on the cases that are biopsies so when its time to print out our log sheets we can do so from the requisitions or working log sheet, and if done right, every case and cassette should match up. I believe Leica now carries these sleeves and metal box containers, I strongly urge you to look into them, they keep things so organized and we LOVE them!! I can get you ordering information, just let me know. Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of bamoe@gundluth.org Sent: Wednesday, August 26, 2009 3:38 PM To: histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - [Histonet] Organizing of cassettes for processing - Email found in subject Hi all - For those that do batch overnight processing, how do you organize the cassettes? Currently we have 2 path assistants that gross throughout the day, and each puts their cassettes as they are grossed into a bucket of formalin. At the end of the day a histotech drains the formalin off, rinses the cassettes in water, then manually puts the cassettes into order according to our worklist, with rush cases being put up front. The baskets are then loaded onto the tissue processor (Sakura VIP5 and VIP6). We are wondering if there are some other ideas of how to streamline this process. One thought was to have the cassettes loaded/organized into the tissue processing basket as they are grossed, but have a concern about formalin exposure while doing this. Any thoughts would be greatly appreciated. Barb Moe Gundersen Lutheran Medical Center La Crosse WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From hymclab.hymclab <@t> ministryhealth.org Thu Aug 27 11:35:32 2009 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Thu Aug 27 11:45:23 2009 Subject: [Histonet] Organizing of cassettes for processing In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF172B39CA@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF172B39CA@EXCHANGESB> Message-ID: We do the same and all of our readings have been well under what they should be. Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 dawn.schneider@ministryhealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, August 27, 2009 8:56 AM To: 'bamoe@gundluth.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Organizing of cassettes for processing We load the cassettes into the tissue processing basket as they are grossed in. They stay in a container of formalin until they are loaded on the tissue processor. What concern about formalin exposure do you have with this method? The gross hood is on while they are doing this. We also monitor our formalin exposure annually and I have the path assistant wear the formalin badge while she performs this task. Our readings have always been well below the acceptable range. If there is a better method I may be interested in it also. Thanks, Carol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of bamoe@gundluth.org Sent: Wednesday, August 26, 2009 4:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Organizing of cassettes for processing Hi all - For those that do batch overnight processing, how do you organize the cassettes? Currently we have 2 path assistants that gross throughout the day, and each puts their cassettes as they are grossed into a bucket of formalin. At the end of the day a histotech drains the formalin off, rinses the cassettes in water, then manually puts the cassettes into order according to our worklist, with rush cases being put up front. The baskets are then loaded onto the tissue processor (Sakura VIP5 and VIP6). We are wondering if there are some other ideas of how to streamline this process. One thought was to have the cassettes loaded/organized into the tissue processing basket as they are grossed, but have a concern about formalin exposure while doing this. Any thoughts would be greatly appreciated. Barb Moe Gundersen Lutheran Medical Center La Crosse WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From TJJ <@t> stowers.org Thu Aug 27 12:18:47 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Thu Aug 27 12:19:43 2009 Subject: [Histonet] Re: DIF tissue in GLUT Message-ID: Anne, Tissue that has been fixed in glutaraldehyde has very, VERY bright autofluorescence. Unless there is some way to minimize this (none that I'm aware of), your immunofluorescence will be impossible to read over the background signal. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From Maria.Katleba <@t> stjoe.org Thu Aug 27 12:40:22 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Thu Aug 27 12:40:33 2009 Subject: [Histonet] Best IHC stainer In-Reply-To: <8CBF53EF6AD28B5-14F0-36B5A@webmail-d004.sysops.aol.com> References: <8CBF53EF6AD28B5-14F0-36B5A@webmail-d004.sysops.aol.com> Message-ID: <97C02552ECB11346877D3E83CF833ABD13D53540A5@SJSNT-SCMAIL03.stjoe.org> I was a DAKO fan for years... But after using Ventana, I completely changed my mind! Sure you pay a little more, but honestly the amount is negligible! In fact, after pricing out Dako versus Ventana, in the end the was really no difference. Plus the quality of the Ventana is exceptional. No one can touch their antibodies... If they don't have the antibody, Cell Marque does. (Cell Marque even puts the antibody into a Ventana style dispenser so that you register it and run!!!) Who else does that? No one. Trust me, I have checked. OMG... now I am sounding like and ad... didn't mean too! I think Ventana is the best.... and you can't beat their customer service dept.. Dako falls short on tech hotline help.... Ventana will even try to help you, even if you have a Dako! I am just saying..... Maria Katleba HT(ASCP) Ms Napa CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: 27 August 2009 08:06 To: gareth.davis@hotmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Best IHC stainer Ventana reagents eem to be a little pricey, but if you want a system that you can load and walk away from, it is the way to go. DAKO is an easy enough to use instrument and is an open system, but they are going to a price per slide kind of thing, if I remmeber correctly. I prefer the IP from Biocare, as is is an open system and a continuous load instrument. And it mixes the chromagen online.... -----Original Message----- From: Gareth Blaeuer Davis To: histonet@lists.utsouthwestern.edu Sent: Wed, Aug 26, 2009 8:04 pm Subject: [Histonet] Best IHC stainer he lab I work in has been doing demos on IHC stainers Ventana Benchmark XT and ako's Autostainer Plus. We could really use feed back from current users of both. We are having a hard time deciding between the two, o any input would be great. What are the Pros and Cons with both. Thanks, Gareth Blaeuer Davis, B.S., HT _________________________________________________________________ otmail? is up to 70% faster. Now good news travels really fast. ttp://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009_______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From wdesalvo.cac <@t> hotmail.com Thu Aug 27 12:51:21 2009 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Thu Aug 27 12:51:24 2009 Subject: [Histonet] Re: DIF tissue in GLUT In-Reply-To: References: Message-ID: I suggest you use a counterstain for your IF to reduce the autofluoresence. Evans Blue - the product can be used as a counterstain in immunohistochemistry when using FITC. After staining for immunofluorescence, dip sections in a 0.1% (w/v) in water solution of Evans Blue for 5-10 minutes. Rinse well in fresh PBS or water before coverslipping. Reference: Immunocytochemistry, Theory and Practice, p. 82 (1988). Purchase from Sigma-Aldrich. William DeSalvo, B.S., HTL(ASCP) > From: TJJ@stowers.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 27 Aug 2009 12:18:47 -0500 > Subject: [Histonet] Re: DIF tissue in GLUT > > Anne, > > Tissue that has been fixed in glutaraldehyde has very, VERY bright autofluorescence. Unless there is some way to minimize this (none that I'm aware of), your immunofluorescence will be impossible to read over the background signal. > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows Live: Keep your friends up to date with what you do online. http://windowslive.com/Campaign/SocialNetworking?ocid=PID23285::T:WLMTAGL:ON:WL:en-US:SI_SB_online:082009 From EdieL <@t> fmchealth.org Thu Aug 27 13:21:34 2009 From: EdieL <@t> fmchealth.org (Edie Lehman) Date: Thu Aug 27 13:21:39 2009 Subject: [Histonet] Toluidine Blue Message-ID: <37BC1F8B840D1F40A11EE5F7F362E5CE040873E342@EX07.fmchealth.org> I am currently writing a procedure for the use of Toluidine Blue staining for both FNA's and frozen sections at the request of one of our pathologists. Can anyone guide me in the use of this stain in lieu of Diff Quick or H&E for rapid processing (such as references I could use, etc) and does anyone have a procedure I can cite for permanent mounting/storage of these slides? Thanks in advance, Edie Lehman MT(ASCP) Anatomical Pathology Supervisor EdieL@fmchealth.org Fairfield Medical Center People you know. Care you trust. Visit us at http://www.fmchealth.org or our online store at http://fairfield.thehospitalstore.com "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." From burch007 <@t> mc.duke.edu Thu Aug 27 13:55:11 2009 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Thu Aug 27 13:55:31 2009 Subject: [Histonet] DIF tissue in GLUT In-Reply-To: Message-ID: Dear Anne, I assume the glutaraldehyde tissue has been processed and embedded in paraffin. Following removal of paraffin and any pretreatment to unmask the antigen, you can block autofluorescence or change the color by treating the sections with 0.05% sodium borohydride prepared in PBS for 30 minutes. After the borohydride treatment, carefully wash the slides with multiple changes of PBS for thirty minutes. I would also use a good quality silane treated slide for maximum tissue adhesion. A comment of caution: sodium borohydride is poisonous. Be extremely careful with the powder. Reference for NaBH4: Embedment in Glycol Methacrylate at Low Temperature Allows Immunofluorescent Localization of a Labile Tissue Protein. JA Carnegie, ME McCully and HA Robertson, Journal of Histochemistry and Cytochemistry, Vol. 28, No. 4, pp 308-310, 1980 Methods in Cell Biology, by Leslie Wilson, page 117, section VI, Use of Glutaraldehyde in Immunofluorescence Microscopy. Academic Press 1982 I used this method in a double label fluorescence project a few years ago with excellent success. 23. Endothelial Metaplasia in the Iridocorneal Endothelial (ICE) Syndrome. DN Howell, T Damms, JL Burchette, JR Ross, WR Green. Investigative Ophthalmology and Visual Science, Vol. 38, No. 9, pages 1896-1901, August 1997. Best Regards, Jim Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" Anne van Binsbergen Sent by: histonet-bounces@lists.utsouthwestern.edu 08/27/2009 06:27 AM To John Kiernan , "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] DIF tissue in GLUT Dear John and other knowledgeable Histonetters and Gurus of the trade.... Renal bx tissue core has been placed in Gluteraldehyde/Trumps EM fixative by mistake (we all make mistakes) - we now need to do DIF Does anyone out there have a method for this? -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> stowers.org Thu Aug 27 14:02:09 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Thu Aug 27 14:03:17 2009 Subject: [Histonet] Re: DIF tissue in GLUT In-Reply-To: Message-ID: William, thanks for the reminder, I had totally forgotten about using a counterstain to counteract AF in aldehyde fixed samples. We have some glutaraldehyde fixed cryosections of kidney I think I'll try this with. I can assure you, the autofluorescence in these samples is magnificent! I worry about using sodium borohydride because they are cryosections and I've heard the treatment can be a bit harsh on tissue. Here's some additional information since we're on the subject: http://www.uhnresearch.ca/facilities/wcif/PDF/Autofluorescence.pdf Kind regards, Teri -----Original Message----- From: WILLIAM DESALVO [mailto:wdesalvo.cac@hotmail.com] Sent: Thursday, August 27, 2009 12:51 PM To: Johnson, Teri; histonet Subject: RE: [Histonet] Re: DIF tissue in GLUT I suggest you use a counterstain for your IF to reduce the autofluoresence. Evans Blue - the product can be used as a counterstain in immunohistochemistry when using FITC. After staining for immunofluorescence, dip sections in a 0.1% (w/v) in water solution of Evans Blue for 5-10 minutes. Rinse well in fresh PBS or water before coverslipping. Reference: Immunocytochemistry, Theory and Practice, p. 82 (1988). Purchase from Sigma-Aldrich. William DeSalvo, B.S., HTL(ASCP) > From: TJJ@stowers.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 27 Aug 2009 12:18:47 -0500 > Subject: [Histonet] Re: DIF tissue in GLUT > > Anne, > > Tissue that has been fixed in glutaraldehyde has very, VERY bright autofluorescence. Unless there is some way to minimize this (none that I'm aware of), your immunofluorescence will be impossible to read over the background signal. > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Windows Live: Keep your friends up to date with what you do online. Find out more. From DKnutson <@t> primecare.org Thu Aug 27 14:18:45 2009 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Thu Aug 27 14:18:56 2009 Subject: [Histonet] (no subject) Message-ID: <4F0B7161A6CD524FAD8017D52E1553400D2B6738@exchangent> I was wondering if anyone on the histonet uses an alcian yellow stain method for H. Pylori? Do you have a problem finding the alcian yellow, or do you use a substitute? I would be interested to hear your pros and cons on this stain. Do you stain manually or use a kit? If a kit, where do you purchase yours from? Thank you all for your insight and knowledge concerning this task. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknutson@primecare.org From Lynne.Bell <@t> cvmc.org Thu Aug 27 14:24:38 2009 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Thu Aug 27 14:24:42 2009 Subject: [Histonet] (no subject) In-Reply-To: <4F0B7161A6CD524FAD8017D52E1553400D2B6738@exchangent> Message-ID: We purchase our Alcian Yellow kit for Helicobacter from Newcomer Supply, Kit #9130. Phone number is 800-383-7799. They are a wonderful company to deal with and their Alcian Yellow kit works beautifully. Our pathologists love it!! Lynne Bell, HT (ASCP) Lead Histologist Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From natecrmr <@t> gmail.com Thu Aug 27 15:44:11 2009 From: natecrmr <@t> gmail.com (Nathan Cramer) Date: Thu Aug 27 15:44:20 2009 Subject: [Histonet] uneven alternating sections on cryostat Message-ID: <4A96F01B.8010902@gmail.com> When cutting PFA fixed, cryoprotected tissue on our cryostat, I frequently find that every other section gets cut improperly. I'll get one nicely cut section and then on the next pass I only get half of a section. This cycle simply repeats over and over and I lose many slices. It has been a while since our cryostat has been serviced, so I'm wondering if this an operator error or a machine problem. I thought maybe the tissue temperature hadn't settled properly, but the uneven cutting still happens even when I let the tissue sit for 30 minutes in the chuck holder. (cutting mouse spinal cord at -20C) On another note, if anyone has any tips for improving white/gray matter contrast in frozen spinal cord sections stained with luxol fast blue, I'd be very appreciative. I do defat the slices with chloroform and differentiate with lithium carbonate but everything is usually either very dark or very light. I am learning as I go (checking the archives here often) so any help would be great. Thanks! Nathan Cramer Neurobiology and Behavior Cornell University Ithaca, NY 14853 From akemiat3377 <@t> yahoo.com Thu Aug 27 15:45:58 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Aug 27 15:46:01 2009 Subject: [Histonet] Best IHC stainer Ventana Ab's Message-ID: <132681.13653.qm@web31303.mail.mud.yahoo.com> Most people are unaware of this, but that's probably because Ventana has had a contract with Cell Marque for years as their primary antibody and other consumables source. --- On Thu, 8/27/09, Maria Katleba wrote: From: Maria Katleba Subject: RE: [Histonet] Best IHC stainer To: "godsgalnow@aol.com" , "gareth.davis@hotmail.com" , "histonet@lists.utsouthwestern.edu" Date: Thursday, August 27, 2009, 10:40 AM I was a DAKO fan for years... But after using Ventana, I completely changed my mind! Sure you pay a little more, but honestly the amount is negligible!? In fact, after pricing out Dako versus Ventana, in the end the was really no difference. Plus the quality of the Ventana is exceptional. No one can touch their antibodies... If they don't have the antibody, Cell Marque does. (Cell Marque even puts the antibody into a Ventana style dispenser so that you register it and run!!!) Who else does that? No one. Trust me, I have checked. OMG... now I am sounding like and ad... didn't mean too!? I think Ventana is the best.... and you can't beat their customer service dept.. Dako falls short on tech hotline help.... Ventana will even try to help you, even if you have a Dako!? I am just saying..... Maria Katleba? HT(ASCP) Ms Napa CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: 27 August 2009 08:06 To: gareth.davis@hotmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Best IHC stainer Ventana reagents eem to be a little pricey, but if you want a system that you can load and walk away from, it is the way to go.? DAKO is an easy enough to use instrument and is an open system, but they are going to a price per slide kind of thing, if I remmeber correctly. I prefer the IP from Biocare, as is is an open system and a continuous load instrument.? And it mixes the chromagen online.... -----Original Message----- From: Gareth Blaeuer Davis To: histonet@lists.utsouthwestern.edu Sent: Wed, Aug 26, 2009 8:04 pm Subject: [Histonet] Best IHC stainer he lab I work in has been doing demos on IHC stainers Ventana Benchmark XT and ako's Autostainer Plus.? We could really use feed back from current users of both.? We are having a hard time deciding between the two, o any input would be great. What are the Pros and Cons with both. Thanks, Gareth Blaeuer Davis, B.S., HT _________________________________________________________________ otmail? is up to 70% faster. Now good news travels really fast. ttp://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009_______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sweething63 <@t> msn.com Thu Aug 27 15:55:16 2009 From: sweething63 <@t> msn.com (R J VAZQUEZ) Date: Thu Aug 27 15:55:21 2009 Subject: [Histonet] uneven alternating sections on cryostat In-Reply-To: <4A96F01B.8010902@gmail.com> References: <4A96F01B.8010902@gmail.com> Message-ID: Nathan, It sounds like the blade holder is not secure enough or even in the tightness on each side. Hope this helps. Robyn > Date: Thu, 27 Aug 2009 16:44:11 -0400 > From: natecrmr@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] uneven alternating sections on cryostat > > When cutting PFA fixed, cryoprotected tissue on our cryostat, I > frequently find that every other section gets cut improperly. I'll get > one nicely cut section and then on the next pass I only get half of a > section. This cycle simply repeats over and over and I lose many slices. > It has been a while since our cryostat has been serviced, so I'm > wondering if this an operator error or a machine problem. I thought > maybe the tissue temperature hadn't settled properly, but the uneven > cutting still happens even when I let the tissue sit for 30 minutes in > the chuck holder. (cutting mouse spinal cord at -20C) > > On another note, if anyone has any tips for improving white/gray matter > contrast in frozen spinal cord sections stained with luxol fast blue, > I'd be very appreciative. I do defat the slices with chloroform and > differentiate with lithium carbonate but everything is usually either > very dark or very light. I am learning as I go (checking the archives > here often) so any help would be great. > > Thanks! > > Nathan Cramer > Neurobiology and Behavior > Cornell University > Ithaca, NY 14853 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Aug 27 16:04:53 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Aug 27 16:04:48 2009 Subject: [Histonet] Best IHC stainer Ventana Ab's In-Reply-To: <132681.13653.qm@web31303.mail.mud.yahoo.com> References: <132681.13653.qm@web31303.mail.mud.yahoo.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA577EF5D@ITSSSXM01V6.one.ads.che.org> Everyone may want to check this out. Heard today that this is no longer the case. Maybe Ventana will make an announcement! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, August 27, 2009 16:46 To: Maria Katleba Cc: histonet Subject: RE: [Histonet] Best IHC stainer Ventana Ab's Most people are unaware of this, but that's probably because Ventana has had a contract with Cell Marque for years as their primary antibody and other consumables source. --- On Thu, 8/27/09, Maria Katleba wrote: From: Maria Katleba Subject: RE: [Histonet] Best IHC stainer To: "godsgalnow@aol.com" , "gareth.davis@hotmail.com" , "histonet@lists.utsouthwestern.edu" Date: Thursday, August 27, 2009, 10:40 AM I was a DAKO fan for years... But after using Ventana, I completely changed my mind! Sure you pay a little more, but honestly the amount is negligible!? In fact, after pricing out Dako versus Ventana, in the end the was really no difference. Plus the quality of the Ventana is exceptional. No one can touch their antibodies... If they don't have the antibody, Cell Marque does. (Cell Marque even puts the antibody into a Ventana style dispenser so that you register it and run!!!) Who else does that? No one. Trust me, I have checked. OMG... now I am sounding like and ad... didn't mean too!? I think Ventana is the best.... and you can't beat their customer service dept.. Dako falls short on tech hotline help.... Ventana will even try to help you, even if you have a Dako!? I am just saying..... Maria Katleba? HT(ASCP) Ms Napa CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: 27 August 2009 08:06 To: gareth.davis@hotmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Best IHC stainer Ventana reagents eem to be a little pricey, but if you want a system that you can load and walk away from, it is the way to go.? DAKO is an easy enough to use instrument and is an open system, but they are going to a price per slide kind of thing, if I remmeber correctly. I prefer the IP from Biocare, as is is an open system and a continuous load instrument.? And it mixes the chromagen online.... -----Original Message----- From: Gareth Blaeuer Davis To: histonet@lists.utsouthwestern.edu Sent: Wed, Aug 26, 2009 8:04 pm Subject: [Histonet] Best IHC stainer he lab I work in has been doing demos on IHC stainers Ventana Benchmark XT and ako's Autostainer Plus.? We could really use feed back from current users of both.? We are having a hard time deciding between the two, o any input would be great. What are the Pros and Cons with both. Thanks, Gareth Blaeuer Davis, B.S., HT _________________________________________________________________ otmail? is up to 70% faster. Now good news travels really fast. ttp://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009_______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From billodonnell <@t> catholichealth.net Thu Aug 27 16:08:41 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Aug 27 16:08:51 2009 Subject: [Histonet] Special stains and microwave In-Reply-To: <4A96F01B.8010902@gmail.com> References: <4A96F01B.8010902@gmail.com> Message-ID: Greetings Histonetters, We have finally come to the point where we need to fish or cut bait in relation to purchasing a laboratory approved microwave and venting it. While I think it would be "cool" to have one, I'm wondering if the cost is justified to do a handful of special stains that could be done (though more slowly) in a laboratory oven like the one we already have. Anyone with the time or desire to opine, please chime in. Those who have purchased such a microwave, I'll gladly take suggestions. Thanks in advance William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 From rfields <@t> gidocs.net Thu Aug 27 16:26:56 2009 From: rfields <@t> gidocs.net (Rosa Fields) Date: Thu Aug 27 16:27:51 2009 Subject: [Histonet] Special stains and microwave References: <4A96F01B.8010902@gmail.com> Message-ID: <07732CE52EC3174AB891DE1C62DB4D8F6F9808@GIEXCHANGE.gidocs.net> Bill, I can highly recommend the EBS processor microwave. At a base price of 8,900 (last I checked) it is one of the lowest cost processors on the market. I don't know if having the flexibility to process in this new microwave may help justify the price? I purchased mine from Stat Lab, and the customer service from the folks at EBS was outstanding; they even had a prob and air agitator block style holder designed and built for me when I suggested that it should come with one. I really like our model, and it offers great flexibility in our lab. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Thursday, August 27, 2009 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special stains and microwave Greetings Histonetters, We have finally come to the point where we need to fish or cut bait in relation to purchasing a laboratory approved microwave and venting it. While I think it would be "cool" to have one, I'm wondering if the cost is justified to do a handful of special stains that could be done (though more slowly) in a laboratory oven like the one we already have. Anyone with the time or desire to opine, please chime in. Those who have purchased such a microwave, I'll gladly take suggestions. Thanks in advance William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From natecrmr <@t> gmail.com Thu Aug 27 16:47:16 2009 From: natecrmr <@t> gmail.com (Nathan Cramer) Date: Thu Aug 27 16:47:27 2009 Subject: [Histonet] uneven alternating sections on cryostat In-Reply-To: References: <4A96F01B.8010902@gmail.com> Message-ID: <4A96FEE4.5080605@gmail.com> Thanks, Robyn... I'll make sure the holder is clamped down. (sometimes its the little things...) Best Nate R J VAZQUEZ wrote: Nathan, It sounds like the blade holder is not secure enough or even in the tightness on each side. Hope this helps. Robyn > Date: Thu, 27 Aug 2009 16:44:11 -0400 > From: [1]natecrmr@gmail.com > To: [2]histonet@lists.utsouthwestern.edu > Subject: [Histonet] uneven alternating sections on cryostat > > When cutting PFA fixed, cryoprotected tissue on our cryostat, I > frequently find that every other section gets cut improperly. I'll get > one nicely cut section and then on the next pass I only get half of a > section. This cycle simply repeats over and over and I lose many slices. > It has been a while since our cryostat has been serviced, so I'm > wondering if this an operator error or a machine problem. I thought > maybe the tissue temperature hadn't settled properly, but the uneven > cutting still happens even when I let the tissue sit for 30 minutes in > the chuck holder. (cutting mouse spinal cord at -20C) > > On another note, if anyone has any tips for improving white/gray matter > contrast in frozen spinal cord sections stained with luxol fast blue, > I'd be very appreciative. I do defat the slices with chloroform and > differentiate with lithium carbonate but everything is usually either > very dark or very light. I am learning as I go (checking the archives > here often) so any help would be great. > > Thanks! > > Nathan Cramer > Neurobiology and Behavior > Cornell University > Ithaca, NY 14853 > > _______________________________________________ > Histonet mailing list > [3]Histonet@lists.utsouthwestern.edu > [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. mailto:natecrmr@gmail.com 2. mailto:histonet@lists.utsouthwestern.edu 3. mailto:Histonet@lists.utsouthwestern.edu 4. http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anonwums1 <@t> gmail.com Thu Aug 27 16:52:56 2009 From: anonwums1 <@t> gmail.com (Adam .) Date: Thu Aug 27 16:53:01 2009 Subject: [Histonet] uneven alternating sections on cryostat In-Reply-To: <4A96FEE4.5080605@gmail.com> References: <4A96F01B.8010902@gmail.com> <4A96FEE4.5080605@gmail.com> Message-ID: <858249120908271452v5a7514d9r749112c480df5831@mail.gmail.com> I was having this problem too, but I think I finally figured it out. There is a screw on our cryostat that attaches the chuck to the rest of the machine. This screws fits into a small hole in the chuck. Sometimes the screw isn't well set in the hole or is well set but for some reason comes loose and causes the chuck to wobble. For some reason, this causes your problem. Now I make sure that everything is tightly secured, and I rarely have this problem. Good luck, Adam On Thu, Aug 27, 2009 at 4:47 PM, Nathan Cramer wrote: > > Thanks, Robyn... I'll make sure the holder is clamped down. (sometimes > its the little things...) > Best > Nate > R J VAZQUEZ wrote: > > Nathan, > It sounds like the blade holder is not secure enough or even in the > tightness on each side. > Hope this helps. > Robyn > > > Date: Thu, 27 Aug 2009 16:44:11 -0400 > > From: [1]natecrmr@gmail.com > > To: [2]histonet@lists.utsouthwestern.edu > > Subject: [Histonet] uneven alternating sections on cryostat > > > > When cutting PFA fixed, cryoprotected tissue on our cryostat, I > > frequently find that every other section gets cut improperly. > I'll get > > one nicely cut section and then on the next pass I only get half > of a > > section. This cycle simply repeats over and over and I lose many > slices. > > It has been a while since our cryostat has been serviced, so I'm > > wondering if this an operator error or a machine problem. I > thought > > maybe the tissue temperature hadn't settled properly, but the > uneven > > cutting still happens even when I let the tissue sit for 30 > minutes in > > the chuck holder. (cutting mouse spinal cord at -20C) > > > > On another note, if anyone has any tips for improving white/gray > matter > > contrast in frozen spinal cord sections stained with luxol fast > blue, > > I'd be very appreciative. I do defat the slices with chloroform > and > > differentiate with lithium carbonate but everything is usually > either > > very dark or very light. I am learning as I go (checking the > archives > > here often) so any help would be great. > > > > Thanks! > > > > Nathan Cramer > > Neurobiology and Behavior > > Cornell University > > Ithaca, NY 14853 > > > > _______________________________________________ > > Histonet mailing list > > [3]Histonet@lists.utsouthwestern.edu > > [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > References > > 1. mailto:natecrmr@gmail.com > 2. mailto:histonet@lists.utsouthwestern.edu > 3. mailto:Histonet@lists.utsouthwestern.edu > 4. http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joseph-galbraith <@t> uiowa.edu Thu Aug 27 17:27:40 2009 From: joseph-galbraith <@t> uiowa.edu (Galbraith, Joe) Date: Thu Aug 27 17:27:52 2009 Subject: [Histonet] uneven alternating sections on cryostat In-Reply-To: <858249120908271452v5a7514d9r749112c480df5831@mail.gmail.com> References: <4A96F01B.8010902@gmail.com><4A96FEE4.5080605@gmail.com> <858249120908271452v5a7514d9r749112c480df5831@mail.gmail.com> Message-ID: Nathan: As indicated by others - first check that everything is tight as that is most often the issue - don't forget to check the adhesion of the specimen to the chuck as well. Also check the angle of approach to the blade as it could be too steep or too shallow. If everything is tight and properly adjusted, then you may have a retraction issue. If your cryostat retracts during the upstroke, the head may still be returning to the proper distance from the head to the specimen during the downstroke and hence you may see no section at first and then a gradually increasing thickness of section during the remainder of the downstroke. This can be due to two causes: 1) You can make this happen by operating the microtome at too high of a speed, if you slow down and the process improves then speed may be a factor; 2) Your microtome may be sticky and require maintenance (defrosting, cleaning, lubrication, etc.). The latter is best left to someone with extensive experience or a service rep. Good luck. Joe Galbraith University of Iowa joseph-galbraith@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adam . Sent: Thursday, August 27, 2009 4:53 PM To: Nathan Cramer Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] uneven alternating sections on cryostat I was having this problem too, but I think I finally figured it out. There is a screw on our cryostat that attaches the chuck to the rest of the machine. This screws fits into a small hole in the chuck. Sometimes the screw isn't well set in the hole or is well set but for some reason comes loose and causes the chuck to wobble. For some reason, this causes your problem. Now I make sure that everything is tightly secured, and I rarely have this problem. Good luck, Adam On Thu, Aug 27, 2009 at 4:47 PM, Nathan Cramer wrote: > > Thanks, Robyn... I'll make sure the holder is clamped down. (sometimes > its the little things...) > Best > Nate > R J VAZQUEZ wrote: > > Nathan, > It sounds like the blade holder is not secure enough or even in the > tightness on each side. > Hope this helps. > Robyn > > > Date: Thu, 27 Aug 2009 16:44:11 -0400 > > From: [1]natecrmr@gmail.com > > To: [2]histonet@lists.utsouthwestern.edu > > Subject: [Histonet] uneven alternating sections on cryostat > > > > When cutting PFA fixed, cryoprotected tissue on our cryostat, I > > frequently find that every other section gets cut improperly. > I'll get > > one nicely cut section and then on the next pass I only get half > of a > > section. This cycle simply repeats over and over and I lose many > slices. > > It has been a while since our cryostat has been serviced, so I'm > > wondering if this an operator error or a machine problem. I > thought > > maybe the tissue temperature hadn't settled properly, but the > uneven > > cutting still happens even when I let the tissue sit for 30 > minutes in > > the chuck holder. (cutting mouse spinal cord at -20C) > > > > On another note, if anyone has any tips for improving white/gray > matter > > contrast in frozen spinal cord sections stained with luxol fast > blue, > > I'd be very appreciative. I do defat the slices with chloroform > and > > differentiate with lithium carbonate but everything is usually > either > > very dark or very light. I am learning as I go (checking the > archives > > here often) so any help would be great. > > > > Thanks! > > > > Nathan Cramer > > Neurobiology and Behavior > > Cornell University > > Ithaca, NY 14853 > > > > _______________________________________________ > > Histonet mailing list > > [3]Histonet@lists.utsouthwestern.edu > > [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > References > > 1. mailto:natecrmr@gmail.com > 2. mailto:histonet@lists.utsouthwestern.edu > 3. mailto:Histonet@lists.utsouthwestern.edu > 4. http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From natecrmr <@t> gmail.com Thu Aug 27 18:21:14 2009 From: natecrmr <@t> gmail.com (Nathan Cramer) Date: Thu Aug 27 18:21:20 2009 Subject: [Histonet] uneven alternating sections on cryostat In-Reply-To: References: <4A96F01B.8010902@gmail.com><4A96FEE4.5080605@gmail.com> <858249120908271452v5a7514d9r749112c480df5831@mail.gmail.com> Message-ID: <4A9714EA.40805@gmail.com> Thanks, Joe and Adam. I'm always paranoid about over tightening and warping some piece of the equipment but maybe I'm being a little too gentle. I'll make sure everything is nice and snug and see how it goes. Happy slicing... Nate Galbraith, Joe wrote: Nathan: As indicated by others - first check that everything is tight as that is most often the issue - don't forget to check the adhesion of the specimen to the chuck as well. Also check the angle of approach to the blade as it could be too steep or too shallow. If everything is tight and properly adjusted, then you may have a retraction issue. If your cryostat retracts during the upstroke, the head may still be returning to the proper distance from the head to the specimen during the downstroke and hence you may see no section at first and then a gradually increasing thickness of section during the remainder of the downstroke. This can be due to two causes: 1) You can make this happen by operating the microtome at too high of a speed, if you slow down and the process improves then speed may be a factor; 2) Your microtome may be sticky and require maintenance (defrosting, cleaning, lubrication, etc.). The latter is best left to someone with extensive experience or a service rep. Good luck. Joe Galbraith University of Iowa [1]joseph-galbraith@uiowa.edu -----Original Message----- From: [2]histonet-bounces@lists.utsouthwestern.edu [[3]mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adam . Sent: Thursday, August 27, 2009 4:53 PM To: Nathan Cramer Cc: [4]histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] uneven alternating sections on cryostat I was having this problem too, but I think I finally figured it out. There is a screw on our cryostat that attaches the chuck to the rest of the machine. This screws fits into a small hole in the chuck. Sometimes the screw isn't well set in the hole or is well set but for some reason comes loose and causes the chuck to wobble. For some reason, this causes your problem. Now I make sure that everything is tightly secured, and I rarely have this problem. Good luck, Adam On Thu, Aug 27, 2009 at 4:47 PM, Nathan Cramer [5] wrote: Thanks, Robyn... I'll make sure the holder is clamped down. (sometimes its the little things...) Best Nate R J VAZQUEZ wrote: Nathan, It sounds like the blade holder is not secure enough or even in the tightness on each side. Hope this helps. Robyn > Date: Thu, 27 Aug 2009 16:44:11 -0400 > From: [[6]1]natecrmr@gmail.com > To: [[7]2]histonet@lists.utsouthwestern.edu > Subject: [Histonet] uneven alternating sections on cryostat > > When cutting PFA fixed, cryoprotected tissue on our cryostat, I > frequently find that every other section gets cut improperly. I'll get > one nicely cut section and then on the next pass I only get half of a > section. This cycle simply repeats over and over and I lose many slices. > It has been a while since our cryostat has been serviced, so I'm > wondering if this an operator error or a machine problem. I thought > maybe the tissue temperature hadn't settled properly, but the uneven > cutting still happens even when I let the tissue sit for 30 minutes in > the chuck holder. (cutting mouse spinal cord at -20C) > > On another note, if anyone has any tips for improving white/gray matter > contrast in frozen spinal cord sections stained with luxol fast blue, > I'd be very appreciative. I do defat the slices with chloroform and > differentiate with lithium carbonate but everything is usually either > very dark or very light. I am learning as I go (checking the archives > here often) so any help would be great. > > Thanks! > > Nathan Cramer > Neurobiology and Behavior > Cornell University > Ithaca, NY 14853 > > _______________________________________________ > Histonet mailing list > [[8]3]Histonet@lists.utsouthwestern.edu > [4][9]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. [10]mailto:natecrmr@gmail.com 2. [11]mailto:histonet@lists.utsouthwestern.edu 3. [12]mailto:Histonet@lists.utsouthwestern.edu 4. [13]http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [14]Histonet@lists.utsouthwestern.edu [15]http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [16]Histonet@lists.utsouthwestern.edu [17]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. mailto:joseph-galbraith@uiowa.edu 2. mailto:histonet-bounces@lists.utsouthwestern.edu 3. mailto:histonet-bounces@lists.utsouthwestern.edu 4. mailto:histonet@lists.utsouthwestern.edu 5. mailto:natecrmr@gmail.com 6. mailto:1]natecrmr@gmail.com 7. mailto:2]histonet@lists.utsouthwestern.edu 8. mailto:3]Histonet@lists.utsouthwestern.edu 9. http://lists.utsouthwestern.edu/mailman/listinfo/histonet 10. mailto:natecrmr@gmail.com 11. mailto:histonet@lists.utsouthwestern.edu 12. mailto:Histonet@lists.utsouthwestern.edu 13. http://lists.utsouthwestern.edu/mailman/listinfo/histonet 14. mailto:Histonet@lists.utsouthwestern.edu 15. http://lists.utsouthwestern.edu/mailman/listinfo/histonet 16. mailto:Histonet@lists.utsouthwestern.edu 17. http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Aug 27 18:27:08 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Aug 27 18:27:11 2009 Subject: [Histonet] Special stains and microwave In-Reply-To: <07732CE52EC3174AB891DE1C62DB4D8F6F9808@GIEXCHANGE.gidocs.net> Message-ID: <248254.42449.qm@web31304.mail.mud.yahoo.com> Hi Rosa, Excuse me if I am wrong, but I think Bill is referring to using a microwave for special stains, and you are referring to a Microwave Tissue Processor.? I have used an oven set at a higher temperature (85 -110 degrees C) for staining the silver portion of the GMS, and it generally only takes between 10-15 minutes.? That is if you place the silver solution in the oven for approximately 10 minutes prior to use.? I also use the oven set at 60-62 degrees C. for the bouins portion of the Trichrome stain.? Although using a water bath at 60 degrees C. is the preferred method.? You have more control using a oven than using a microwave.? With a microwave, results vary with the # of slides placed in the coplin jar, as well as the placement in the microwave.? There are variations in microwave intensities if you don't have a carousel.? You also may have boil over of solutions.? Food for thought. Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com --- On Thu, 8/27/09, Rosa Fields wrote: From: Rosa Fields Subject: RE: [Histonet] Special stains and microwave To: "O'Donnell, Bill" , histonet@lists.utsouthwestern.edu Date: Thursday, August 27, 2009, 2:26 PM Bill, I can highly recommend the EBS processor microwave.? At a base price of 8,900 (last I checked) it is one of the lowest cost processors on the market.? I don't know if having the flexibility to process in this new microwave may help justify the price?? I purchased mine from Stat Lab, and the customer service from the folks at EBS was outstanding; they even had a prob and air agitator block style holder designed and built for me when I suggested that it should come with one.? I really like our model, and it offers great flexibility in our lab.? Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE? 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Thursday, August 27, 2009 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special stains and microwave Greetings Histonetters, We have finally come to the point where we need to fish or cut bait in relation to purchasing a laboratory approved microwave and venting it. While I think it would be "cool" to have one, I'm wondering if the cost is justified to do a handful of special stains that could be done (though more slowly) in a laboratory oven like the one we already have. Anyone with the time or desire to opine, please chime in. Those who have purchased such a microwave, I'll gladly take suggestions. Thanks in advance William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Fri Aug 28 08:00:33 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Aug 28 08:00:39 2009 Subject: [Histonet] RE: Special stains and microwave In-Reply-To: References: <4A96F01B.8010902@gmail.com> Message-ID: We had one and we really didn't use it often enough to justify the cost. Maybe 2 or 3 stains benefited from it, otherwise waterbath and incubators were the preferred method for most of us. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Thursday, August 27, 2009 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special stains and microwave Greetings Histonetters, We have finally come to the point where we need to fish or cut bait in relation to purchasing a laboratory approved microwave and venting it. While I think it would be "cool" to have one, I'm wondering if the cost is justified to do a handful of special stains that could be done (though more slowly) in a laboratory oven like the one we already have. Anyone with the time or desire to opine, please chime in. Those who have purchased such a microwave, I'll gladly take suggestions. Thanks in advance William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From rsrichmond <@t> gmail.com Fri Aug 28 08:41:25 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Fri Aug 28 08:41:29 2009 Subject: [Histonet] Re: Toluidine Blue Message-ID: Edie Lehman MT(ASCP), Anatomical Pathology Supervisor at Fairfield Medical Center in Lancaster, Ohio asks: >>I am currently writing a procedure for the use of Toluidine Blue staining for both FNA's and frozen sections at the request of one of our pathologists. Can anyone guide me in the use of this stain in lieu of Diff-Quik or H&E for rapid processing (such as references I could use, etc) and does anyone have a procedure I can cite for permanent mounting/storage of these slides?<< If you want to prepare a suitably buffered solution of toluidine blue for frozen sections, you'll have to look up a formula. If you don't want to do this, write your procedure for Diff-Quik II (the blue solution) or one of a number of generic equivalents available. (Don't use a combined stain such as Sigma's for this purpose, unless you also want eosin.) Stain for a few seconds (slosh the slide through the stain till it's evenly wetted, dehydrate rapidly through alcohols, then into xylene (or substitute), mount in resin. Control slides are usually unnecessary, and are an impediment to getting the frozen section done rapidly. If the pathologist wants the eosin also (Diff-Quik I or generic equivalent), then follow the package insert. Diff-Quik (note the spelling) is a trademark of whatever Scientific Products is called this week. I would use one of the standard pathology books on frozen sections - your pathologist should have a copy - as a reference, just to have something on paper. Bob Richmond Samurai Pathologist Knoxville TN From TJJ <@t> stowers.org Fri Aug 28 08:44:04 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Fri Aug 28 08:45:13 2009 Subject: [Histonet] Re: DIF tissue in GLUT Message-ID: Jim, unless they've changed methodologies since I left clinical, they process all DIF for renal biopsies for cryosectioning. I'm very aware of the different methods for quenching autofluorescence, and I know that what works for some samples doesn't work entirely for all. Additionally, what may work for formalin induced AF may not even touch GLUT induced AF. Best thing to do is try various methods, and see what works. Even more important is to provide positive and negative controls (both treated with the chemical agents for AF) for this case so you can be reasonably certain that if you have diagnostic staining that's consistent with the controls, that it is real. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From sprice2003 <@t> gmail.com Fri Aug 28 08:50:13 2009 From: sprice2003 <@t> gmail.com (Sally Price) Date: Fri Aug 28 08:50:17 2009 Subject: [Histonet] Double HRP immunostains Message-ID: Andrea - We love Biocare's Bajoran Purple. It's out of this world...or it galaxy? Sally ------------------------------ Message: 7 Date: Wed, 26 Aug 2009 14:05:41 -0400 From: Andrea Hooper Subject: [Histonet] Double HRP immunostains To: Histonet histonet@lists.utsouthwestern.edu I do double HRP immunostains. Works well using DAB/AEC and True Blue... but what are your favorite chromagens to use? Would love some feedback for inspiration. Andrea -- From christi.cosby <@t> gmail.com Fri Aug 28 08:52:45 2009 From: christi.cosby <@t> gmail.com (Christi Cosby) Date: Fri Aug 28 08:52:49 2009 Subject: [Histonet] MART-1 on paraffin sections Message-ID: I've been searching pubmed, and I can't find any protocols for performing a MART-1 stain on paraffin-embedded skin sections taken from Mohs surgery. Has anyone tried this? I would appreciate any advice! Christi Cosby Dermatology- Surgical Oncology UT Southwestern From TJJ <@t> stowers.org Fri Aug 28 08:55:37 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Fri Aug 28 08:56:31 2009 Subject: [Histonet] Re: DIF in GLUT Message-ID: P.S. Jim, thanks for the references, I'm on my way to dig them out as we speak. Cheers! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From rahayes <@t> serha.ca Fri Aug 28 08:57:44 2009 From: rahayes <@t> serha.ca (Hayes, Randi (R1SE)) Date: Fri Aug 28 08:57:50 2009 Subject: [Histonet] Best IHC Stainer Message-ID: A stainer is only as good as its antibodies. We've been less than impressed with the quality of Cell Marque antibodies as well as their customer service. Contact me if you want specifics. What I would suggest is when looking for "the best", look for the antibodies that rank the most robust on external EQA surveys (UK Nequas, NordiQC as examples) and go with a system that gives you the flexibility (within reasonable cost) to use the most reliable antibodies. We are Ventana XT users and have been for over 5 years. It provides good staining but with little flexibility to use other vendors antibodies unless you want to completely blow your budget. This forces you to use Cell Marque products and I've already shared my opinion on that. Randi Hayes Histology Supervisor The Moncton Hospital Regional Health Authority B Moncton, NB randi.hayes@serha.ca ----- SERHA Disclaimer ----- This electronic transmission and any accompanying attachments may contain privileged or confidential information intended only for the use of the individual or organization named above. Any distribution, copying or action taken in reliance on the contents of this communication by anyone other than the intended recipient(s) is STRICTLY PROHIBITED. If you have received this communication in error please notify the sender at the above e-mail address and delete this e-mail immediately. Thank you. Cette communication ?lectronique et toute pi?ce jointe peuvent contenir de l'information de nature privil?gi?e ou confidentielle et sont strictement r?serv?es ? l'usage du destinataire vis? et identifi? ci-dessus. Si vous n'?tes pas le destinataire vis?, prenez avis que toute distribution, reproduction ou mesure fond?e sur l'information qui y est contenue est EXPRESS?MENT INTERDITE. Si vous avez re?u cette communication par erreur, veuillez en aviser imm?diatement l'exp?diteur par courriel (? l'adresse ?lectronique mentionn?e ci-dessus) et supprimer le message d'origine. Merci. From TJJ <@t> stowers.org Fri Aug 28 09:15:08 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Fri Aug 28 09:15:57 2009 Subject: [Histonet] Re: uneven alternating sections on cryostat Message-ID: Nathan, you got great information already. Be careful to make sure things are snug, but don't really clamp down on the holders. It's just not necessary. It just needs to be sturdy and not moveable. If everything appears to be snug, then it's likely knife angle. Try making an OCT block and change the angle a little at time until you achieve complete, even, continuous sections. Then try it on your sample. I'm not a neurohistotechnician but I'm sure there are some out there who can help with your LFB staining problem. I think a thickness of approx. 8-10 microns are recommended for sections for this stain. You might be seeing variability due to the thin/thick issues you're having, not due to any staining problem. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From richardsje <@t> verizon.net Fri Aug 28 09:39:59 2009 From: richardsje <@t> verizon.net (richardsje@verizon.net) Date: Fri Aug 28 09:40:08 2009 Subject: [Histonet] Dako Autostainer Message-ID: <1423443284.694606.1251470399584.JavaMail.root@vms184.mailsrvcs.net> I was wondering if anyone had any comments/concerns about the Dako Autostainer? My lab currently has a Ventana Benchmark LT, but we are looking to switch. Thanks! From talulahgosh <@t> gmail.com Fri Aug 28 09:54:02 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Aug 28 09:54:06 2009 Subject: [Histonet] Re: uneven alternating sections on cryostat In-Reply-To: References: Message-ID: You've gotten great advice so far, but if it doesn't help--our problem was that the specimen head was loose--even when it was locked, it would move very slightly. This has to be fixed by servicing it, unfortunately. It was caused by our MD/PhD student trying to adjust the specimen head when it was locked, hence loosening the screws. It happened even though I told the guy to quit doing it, which drove me mad. How some people get this far is beyond me. Emily "One of the defining characteristics of modern surgery was that patients ought to survive it." --Peter Stanley, For Fear of Pain: British Surgery, 1790-1850 From LSebree <@t> uwhealth.org Fri Aug 28 10:02:38 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Aug 28 10:02:53 2009 Subject: [Histonet] Best IHC Stainer In-Reply-To: Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A5890357068C@UWHC-MAIL01.uwhis.hosp.wisc.edu> Randi, We also use Ventana instruments (3) but use whose ever antibodies make sense for our work situation. You don't need to "blow your budget" especially if you go with concentrate antibodies and purchase user fillable dispensers from Ventana. Basically there are no limitations for which antibodies you use with Ventana instruments. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hayes, Randi (R1SE) Sent: Friday, August 28, 2009 8:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Best IHC Stainer A stainer is only as good as its antibodies. We've been less than impressed with the quality of Cell Marque antibodies as well as their customer service. Contact me if you want specifics. What I would suggest is when looking for "the best", look for the antibodies that rank the most robust on external EQA surveys (UK Nequas, NordiQC as examples) and go with a system that gives you the flexibility (within reasonable cost) to use the most reliable antibodies. We are Ventana XT users and have been for over 5 years. It provides good staining but with little flexibility to use other vendors antibodies unless you want to completely blow your budget. This forces you to use Cell Marque products and I've already shared my opinion on that. Randi Hayes Histology Supervisor The Moncton Hospital Regional Health Authority B Moncton, NB randi.hayes@serha.ca ----- SERHA Disclaimer ----- This electronic transmission and any accompanying attachments may contain privileged or confidential information intended only for the use of the individual or organization named above. Any distribution, copying or action taken in reliance on the contents of this communication by anyone other than the intended recipient(s) is STRICTLY PROHIBITED. If you have received this communication in error please notify the sender at the above e-mail address and delete this e-mail immediately. Thank you. Cette communication ?lectronique et toute pi?ce jointe peuvent contenir de l'information de nature privil?gi?e ou confidentielle et sont strictement r?serv?es ? l'usage du destinataire vis? et identifi? ci-dessus. Si vous n'?tes pas le destinataire vis?, prenez avis que toute distribution, reproduction ou mesure fond?e sur l'information qui y est contenue est EXPRESS?MENT INTERDITE. Si vous avez re?u cette communication par erreur, veuillez en aviser imm?diatement l'exp?diteur par courriel (? l'adresse ?lectronique mentionn?e ci-dessus) et supprimer le message d'origine. Merci. From eridana <@t> cox.net Fri Aug 28 10:58:47 2009 From: eridana <@t> cox.net (Eridana) Date: Fri Aug 28 10:58:59 2009 Subject: [Histonet] Cryostat thick and thin sections Message-ID: <20090828115847.M2L87.136504.imail@fed1rmwml28> One more possible issue along with the others. Over tightening of some disposable blades can cause thick and thin sections. Leica 1800 and 3050 only should have the knife tightened snug, not have the handle (tightening bar or whatever you call it) pushed as far as it goes. My rule of thumb is to go just beyond the angle of the knife and quit there. I love the 3050- it is my favorite for any cryo work. Donna Harclerode HT, HTL, (ASCP),QIHC, SLS From Maria.Katleba <@t> stjoe.org Fri Aug 28 11:24:20 2009 From: Maria.Katleba <@t> stjoe.org (Maria Katleba) Date: Fri Aug 28 11:24:27 2009 Subject: [Histonet] Best IHC stainer Ventana Ab's In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA577EF5D@ITSSSXM01V6.one.ads.che.org> References: <132681.13653.qm@web31303.mail.mud.yahoo.com> <5D64396A0D4A5346BEBC759022AAEAA577EF5D@ITSSSXM01V6.one.ads.che.org> Message-ID: <97C02552ECB11346877D3E83CF833ABD13D535448A@SJSNT-SCMAIL03.stjoe.org> What announcement? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: 27 August 2009 14:05 Cc: histonet Subject: RE: [Histonet] Best IHC stainer Ventana Ab's Everyone may want to check this out. Heard today that this is no longer the case. Maybe Ventana will make an announcement! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, August 27, 2009 16:46 To: Maria Katleba Cc: histonet Subject: RE: [Histonet] Best IHC stainer Ventana Ab's Most people are unaware of this, but that's probably because Ventana has had a contract with Cell Marque for years as their primary antibody and other consumables source. --- On Thu, 8/27/09, Maria Katleba wrote: From: Maria Katleba Subject: RE: [Histonet] Best IHC stainer To: "godsgalnow@aol.com" , "gareth.davis@hotmail.com" , "histonet@lists.utsouthwestern.edu" Date: Thursday, August 27, 2009, 10:40 AM I was a DAKO fan for years... But after using Ventana, I completely changed my mind! Sure you pay a little more, but honestly the amount is negligible! In fact, after pricing out Dako versus Ventana, in the end the was really no difference. Plus the quality of the Ventana is exceptional. No one can touch their antibodies... If they don't have the antibody, Cell Marque does. (Cell Marque even puts the antibody into a Ventana style dispenser so that you register it and run!!!) Who else does that? No one. Trust me, I have checked. OMG... now I am sounding like and ad... didn't mean too! I think Ventana is the best.... and you can't beat their customer service dept.. Dako falls short on tech hotline help.... Ventana will even try to help you, even if you have a Dako! I am just saying..... Maria Katleba HT(ASCP) Ms Napa CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: 27 August 2009 08:06 To: gareth.davis@hotmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Best IHC stainer Ventana reagents eem to be a little pricey, but if you want a system that you can load and walk away from, it is the way to go. DAKO is an easy enough to use instrument and is an open system, but they are going to a price per slide kind of thing, if I remmeber correctly. I prefer the IP from Biocare, as is is an open system and a continuous load instrument. And it mixes the chromagen online.... -----Original Message----- From: Gareth Blaeuer Davis To: histonet@lists.utsouthwestern.edu Sent: Wed, Aug 26, 2009 8:04 pm Subject: [Histonet] Best IHC stainer he lab I work in has been doing demos on IHC stainers Ventana Benchmark XT and ako's Autostainer Plus. We could really use feed back from current users of both. We are having a hard time deciding between the two, o any input would be great. What are the Pros and Cons with both. Thanks, Gareth Blaeuer Davis, B.S., HT _________________________________________________________________ otmail? is up to 70% faster. Now good news travels really fast. ttp://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009_______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. From Rcartun <@t> harthosp.org Fri Aug 28 13:32:00 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Aug 28 13:32:08 2009 Subject: [Histonet] COX-2 Message-ID: <4A97EA5F.7400.0077.1@harthosp.org> There was a recent study that showed "aspirin" affected the growth of one type of colorectal cancer; that which overexpresses COX-2. If this holds up, get ready to start doing a lot of COX-2 IHC. Does anyone have a good mAb to COX-2 that works on formalin-fixed, paraffin-embedded tissue that they would recommend? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From lpaveli1 <@t> hurleymc.com Fri Aug 28 13:37:16 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Fri Aug 28 13:37:30 2009 Subject: [Histonet] COX-2 Message-ID: <4A97EB9C020000EE0002BFF1@smtp-gw.hurleymc.com> Could you share the source of the study? thank you, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Richard Cartun" 08/28/09 2:32 PM >>> There was a recent study that showed "aspirin" affected the growth of one type of colorectal cancer; that which overexpresses COX-2. If this holds up, get ready to start doing a lot of COX-2 IHC. Does anyone have a good mAb to COX-2 that works on formalin-fixed, paraffin-embedded tissue that they would recommend? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rfields <@t> gidocs.net Fri Aug 28 13:42:13 2009 From: rfields <@t> gidocs.net (Rosa Fields) Date: Fri Aug 28 13:43:13 2009 Subject: [Histonet] Special stains and microwave References: <248254.42449.qm@web31304.mail.mud.yahoo.com> Message-ID: <07732CE52EC3174AB891DE1C62DB4D8F6F980A@GIEXCHANGE.gidocs.net> You are correct, Bill was referring to using the microwave for special stains, I just thought it may be easier to justify the cost of a lab microwave if you looked at being able to use it as a processor also. A lab grade microwave is far better than your typical kitchen microwave, which will produce hot and cold spots, and staining variability because of them, they also lack the ability to have consistent, repeatable results. A good lab microwave needs to have short magnetron cycle time, or better yet a continuous power, and for any consistent results have a timer that starts at temperature control. It should also have both a temperature probe and some sort of agitation, either a "bubbler wand" or other method to move the solution around for consistent temperature. Once you have control over these issues, you can produce nicely stained, consistent results with rapid TAT. Having a processer that can process biopsy's in 16 mins is a nice benefit also! Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited. From: Akemi Allison-Tacha [mailto:akemiat3377@yahoo.com] Sent: Thursday, August 27, 2009 6:27 PM To: BillO'Donnell; histonet@lists.utsouthwestern.edu; Rosa Fields Subject: RE: [Histonet] Special stains and microwave Hi Rosa, Excuse me if I am wrong, but I think Bill is referring to using a microwave for special stains, and you are referring to a Microwave Tissue Processor. I have used an oven set at a higher temperature (85 -110 degrees C) for staining the silver portion of the GMS, and it generally only takes between 10-15 minutes. That is if you place the silver solution in the oven for approximately 10 minutes prior to use. I also use the oven set at 60-62 degrees C. for the bouins portion of the Trichrome stain. Although using a water bath at 60 degrees C. is the preferred method. You have more control using a oven than using a microwave. With a microwave, results vary with the # of slides placed in the coplin jar, as well as the placement in the microwave. There are variations in microwave intensities if you don't have a carousel. You also may have boil over of solutions. Food for thought. Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-tacha@apmglab.com (P) E-Mail: akemiat3377@yahoo.com --- On Thu, 8/27/09, Rosa Fields wrote: From: Rosa Fields Subject: RE: [Histonet] Special stains and microwave To: "O'Donnell, Bill" , histonet@lists.utsouthwestern.edu Date: Thursday, August 27, 2009, 2:26 PM Bill, I can highly recommend the EBS processor microwave. At a base price of 8,900 (last I checked) it is one of the lowest cost processors on the market. I don't know if having the flexibility to process in this new microwave may help justify the price? I purchased mine from Stat Lab, and the customer service from the folks at EBS was outstanding; they even had a prob and air agitator block style holder designed and built for me when I suggested that it should come with one. I really like our model, and it offers great flexibility in our lab. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Thursday, August 27, 2009 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special stains and microwave Greetings Histonetters, We have finally come to the point where we need to fish or cut bait in relation to purchasing a laboratory approved microwave and venting it. While I think it would be "cool" to have one, I'm wondering if the cost is justified to do a handful of special stains that could be done (though more slowly) in a laboratory oven like the one we already have. Anyone with the time or desire to opine, please chime in. Those who have purchased such a microwave, I'll gladly take suggestions. Thanks in advance William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLunetta <@t> luhcares.org Fri Aug 28 16:37:32 2009 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Fri Aug 28 16:38:09 2009 Subject: [Histonet] Re: Dako Autostainer Message-ID: <4A97F9BC020000A800035F3C@mail.luhcares.org> Richard, We have just up-graded to the new Dako Autostainer 48 and after some growing/switching pains (Dako was very responsive in the fixing issues) it is great. The instrument gives very nice consistent results and is also an open system that allows the easy use of other manufactures anti-bodies. If you can call your local rep and get a demo. Matt Lunetta BS HT (ASCP) Longmont United Hospital Colorado Message: 8 Date: Fri, 28 Aug 2009 09:39:59 -0500 (CDT) From: richardsje@verizon.net Subject: [Histonet] Dako Autostainer To: histonet@lists.utsouthwestern.edu Message-ID: <1423443284.694606.1251470399584.JavaMail.root@vms184.mailsrvcs.net><~!B*+R^&>Content-Type: text/plain; charset="UTF-8" I was wondering if anyone had any comments/concerns about the Dako Autostainer? My lab currently has a Ventana Benchmark LT, but we are looking to switch. Thanks! From amosbrooks <@t> gmail.com Fri Aug 28 18:48:48 2009 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Aug 28 18:48:53 2009 Subject: [Histonet] Best IHC Stainer Message-ID: <582736990908281648x7f15d05ag76d25df1c47746c7@mail.gmail.com> Hi Randi, The statement that a stainer is only as good as it's antibodies is nails on a chalk board to me. You can get a good stainer and if the company has bad antibodies ... *change them!* No one company can do it all. If they tell you otherwise RUN they are lieing to you. The definition of a good instrument is one that allows you the flexability to use whatever antibodies you need to use. If you need to run an antibody that isn't offered by the company that sells your instrument, there are thousands of other antibody companies to choose from. If the antibody requires the use of species of secondary that is not offered by the company and the company doesn't allow you to use something else, it is not worth using. Instrumentation is a function of good engineering and software programming. It has little if anything to do with the biochemistry of antibody applications. I really don't mean to sound sharp here, but there are too many people that think that the company that sells their instrument is the only company that they can use. If this is the case with any instrument the customer got shafted in buying the instrument. Amos Message: 6 Date: Fri, 28 Aug 2009 10:57:44 -0300 From: "Hayes, Randi (R1SE)" Subject: [Histonet] Best IHC Stainer To: Message-ID: Content-Type: text/plain; charset="utf-8" A stainer is only as good as its antibodies. We've been less than impressed with the quality of Cell Marque antibodies as well as their customer service. Contact me if you want specifics. What I would suggest is when looking for "the best", look for the antibodies that rank the most robust on external EQA surveys (UK Nequas, NordiQC as examples) and go with a system that gives you the flexibility (within reasonable cost) to use the most reliable antibodies. We are Ventana XT users and have been for over 5 years. It provides good staining but with little flexibility to use other vendors antibodies unless you want to completely blow your budget. This forces you to use Cell Marque products and I've already shared my opinion on that. Randi Hayes Histology Supervisor The Moncton Hospital Regional Health Authority B Moncton, NB From histopatty <@t> aol.com Fri Aug 28 22:36:10 2009 From: histopatty <@t> aol.com (histopatty@aol.com) Date: Fri Aug 28 22:36:25 2009 Subject: [Histonet] EM processing Message-ID: <8CBF670FC352747-2ACC-10E5C@webmail-m043.sysops.aol.com> Does your lab make their own plastic resin to process and embed specimens or do you buy it commercially? What is the average time it take to process a specimen? Thanks in advance to all who respond. P.Eneff OU Medical Center Oklahoma City, OK From sabeti_shahram <@t> yahoo.com Sat Aug 29 02:48:23 2009 From: sabeti_shahram <@t> yahoo.com (Shahram Sabeti) Date: Sat Aug 29 02:48:32 2009 Subject: [Histonet] h.pylori silver staining Message-ID: <652238.69300.qm@web35605.mail.mud.yahoo.com> hello dear colleagues ??? i have heard of a new modified silver staining method for H.pylori.do you know anything about it and it's methology.please inform me if possible. ?????????????????????????????????????????????????????????????????????????????????????????????? yours ???????????????????????????????????????????????????????????????????????????????????????????????sabeti? From Rcartun <@t> harthosp.org Sat Aug 29 09:14:16 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sat Aug 29 09:14:23 2009 Subject: [Histonet] COX-2 In-Reply-To: <4A97EB9C020000EE0002BFF1@smtp-gw.hurleymc.com> References: <4A97EB9C020000EE0002BFF1@smtp-gw.hurleymc.com> Message-ID: <4A98FF77.7400.0077.1@harthosp.org> It was in "USA Today", but will be published in this week's issue of JAMA. RWC Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Lynette Pavelich" 8/28/2009 2:37 PM >>> Could you share the source of the study? thank you, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Richard Cartun" 08/28/09 2:32 PM >>> There was a recent study that showed "aspirin" affected the growth of one type of colorectal cancer; that which overexpresses COX-2. If this holds up, get ready to start doing a lot of COX-2 IHC. Does anyone have a good mAb to COX-2 that works on formalin-fixed, paraffin-embedded tissue that they would recommend? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Aug 29 09:32:10 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Aug 29 09:32:14 2009 Subject: [Histonet] h.pylori silver staining In-Reply-To: <652238.69300.qm@web35605.mail.mud.yahoo.com> Message-ID: <868447.75422.qm@web65712.mail.ac4.yahoo.com> Shahram: Under separate cover I am sending you the procedure. Ren? J. --- On Sat, 8/29/09, Shahram Sabeti wrote: From: Shahram Sabeti Subject: [Histonet] h.pylori silver staining To: histonet@lists.utsouthwestern.edu Date: Saturday, August 29, 2009, 3:48 AM hello dear colleagues ??? i have heard of a new modified silver staining method for H.pylori.do you know anything about it and it's methology.please inform me if possible. ?????????????????????????????????????????????????????????????????????????????????????????????? yours ???????????????????????????????????????????????????????????????????????????????????????????????sabeti? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Aug 29 09:43:50 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Aug 29 09:45:14 2009 Subject: SPAM-LOW: Re: [Histonet] COX-2 In-Reply-To: <4A98FF77.7400.0077.1@harthosp.org> References: <4A97EB9C020000EE0002BFF1@smtp-gw.hurleymc.com> <4A98FF77.7400.0077.1@harthosp.org> Message-ID: <61631E09A2FC4A298D393729097215AD@prueggihctechlt> Richard, I did a lot of work on cox2 a while ago on ffpe tissue and I was not ever able to get results I was satisfied with. I would be really interested in this as well, sometimes it just takes finding the right clone and the right pretreatment, I am not there yet with cox2 even though several people gave me their protocols I could not get them to work well. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Saturday, August 29, 2009 8:14 AM To: Lynette Pavelich; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] COX-2 It was in "USA Today", but will be published in this week's issue of JAMA. RWC Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Lynette Pavelich" 8/28/2009 2:37 PM >>> Could you share the source of the study? thank you, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor MSH Competency Coordinator Hurley Medical Center One Hurley Plaza Flint, MI 48503 email: Lpaveli1@hurleymc.com ph: 810-257-9948 fax: 810-762-7082 >>> "Richard Cartun" 08/28/09 2:32 PM >>> There was a recent study that showed "aspirin" affected the growth of one type of colorectal cancer; that which overexpresses COX-2. If this holds up, get ready to start doing a lot of COX-2 IHC. Does anyone have a good mAb to COX-2 that works on formalin-fixed, paraffin-embedded tissue that they would recommend? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Aug 29 09:53:10 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Aug 29 09:53:50 2009 Subject: SPAM-LOW: [Histonet] Best IHC Stainer In-Reply-To: <582736990908281648x7f15d05ag76d25df1c47746c7@mail.gmail.com> References: <582736990908281648x7f15d05ag76d25df1c47746c7@mail.gmail.com> Message-ID: I agree with you Amos, but some instruments are more friendly to using other's antibodies and detections than others. In my opinion the Dako autostainer, Leica Bond and the BC IHC autostainers are very good open systems, and even some of them are better at supporting animal and human research. Patsy Patsy Ruegg, HT(ASCP)QIhc IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, August 28, 2009 5:49 PM To: rahayes@serha.ca; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Best IHC Stainer Hi Randi, The statement that a stainer is only as good as it's antibodies is nails on a chalk board to me. You can get a good stainer and if the company has bad antibodies ... *change them!* No one company can do it all. If they tell you otherwise RUN they are lieing to you. The definition of a good instrument is one that allows you the flexability to use whatever antibodies you need to use. If you need to run an antibody that isn't offered by the company that sells your instrument, there are thousands of other antibody companies to choose from. If the antibody requires the use of species of secondary that is not offered by the company and the company doesn't allow you to use something else, it is not worth using. Instrumentation is a function of good engineering and software programming. It has little if anything to do with the biochemistry of antibody applications. I really don't mean to sound sharp here, but there are too many people that think that the company that sells their instrument is the only company that they can use. If this is the case with any instrument the customer got shafted in buying the instrument. Amos Message: 6 Date: Fri, 28 Aug 2009 10:57:44 -0300 From: "Hayes, Randi (R1SE)" Subject: [Histonet] Best IHC Stainer To: Message-ID: Content-Type: text/plain; charset="utf-8" A stainer is only as good as its antibodies. We've been less than impressed with the quality of Cell Marque antibodies as well as their customer service. Contact me if you want specifics. What I would suggest is when looking for "the best", look for the antibodies that rank the most robust on external EQA surveys (UK Nequas, NordiQC as examples) and go with a system that gives you the flexibility (within reasonable cost) to use the most reliable antibodies. We are Ventana XT users and have been for over 5 years. It provides good staining but with little flexibility to use other vendors antibodies unless you want to completely blow your budget. This forces you to use Cell Marque products and I've already shared my opinion on that. Randi Hayes Histology Supervisor The Moncton Hospital Regional Health Authority B Moncton, NB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aazath <@t> hotmail.com Sat Aug 29 12:51:21 2009 From: aazath <@t> hotmail.com (Aazath Raj) Date: Sat Aug 29 12:51:25 2009 Subject: [Histonet] (no subject) Message-ID: Dear All, we have got a new leica multistainer ST 5020.we are using it for both H&E and as well as PAP.Out of 34 station i have only 15 station for H&E.Is anybody having a Staining protocol for H&E which fits in 15 station with 2 water wash bath. Aazath Technical Officer Apollo Hospitals Chennai India _________________________________________________________________ Log on to MSN India for a lowdown on what?s hot in the world today http://in.msn.com From gu.lang <@t> gmx.at Sat Aug 29 13:06:21 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Aug 29 13:06:29 2009 Subject: AW: [Histonet] (no subject) In-Reply-To: References: Message-ID: <3AE070F313CA48219D4BCB10C5A69A3D@dielangs.at> Hi Aazath, This is our HE protocol, it's for 16 stations. And I'm interested in your PAP-protocol. Would you be so nice and send it to me? 25 min oven 2 x 5 min Xylol-substitute 1 x 1 min 96% 1 x 1 min 80% 1 x 1 min 50% 1 x 1 min dest. water 1 x 10 min Mayers Hemalaun (exact) 1 x 5 min tapwater running 1 x 30 sek 2% Eosin in water (exact) 1 x 30 sek tapwater running (exact) 1 x 30 sek 96% (exact) 3 x 1 min 100% 2 x Xylol-substitute Coverslipper The times without "exact" are minimum, "dip" is on with all stations. Alcohols and Xylol-subst. are changed every day. Hope this helps Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Aazath Raj Gesendet: Samstag, 29. August 2009 19:51 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] (no subject) Dear All, we have got a new leica multistainer ST 5020.we are using it for both H&E and as well as PAP.Out of 34 station i have only 15 station for H&E.Is anybody having a Staining protocol for H&E which fits in 15 station with 2 water wash bath. Aazath Technical Officer Apollo Hospitals Chennai India _________________________________________________________________ Log on to MSN India for a lowdown on what?s hot in the world today http://in.msn.com_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Sat Aug 29 13:38:35 2009 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Sat Aug 29 13:38:40 2009 Subject: [Histonet] h.pylori silver staining In-Reply-To: <868447.75422.qm@web65712.mail.ac4.yahoo.com> References: <652238.69300.qm@web35605.mail.mud.yahoo.com> <868447.75422.qm@web65712.mail.ac4.yahoo.com> Message-ID: A Warthin-Starry procedure works great. The kit and procedure on the DAKO Artisan is fast, reliable and provides good results. William DeSalvo, B.S., HTL(ASCP) > Date: Sat, 29 Aug 2009 07:32:10 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; sabeti_shahram@yahoo.com > Subject: Re: [Histonet] h.pylori silver staining > CC: > > Shahram: > Under separate cover I am sending you the procedure. > Ren? J. > > --- On Sat, 8/29/09, Shahram Sabeti wrote: > > > From: Shahram Sabeti > Subject: [Histonet] h.pylori silver staining > To: histonet@lists.utsouthwestern.edu > Date: Saturday, August 29, 2009, 3:48 AM > > > hello dear colleagues > i have heard of a new modified silver staining method for H.pylori.do you know anything about it and it's methology.please inform me if possible. > yours > sabeti > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009 From vapatpxs <@t> yahoo.com Sat Aug 29 15:18:38 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Sat Aug 29 15:18:42 2009 Subject: [Histonet] EM processing In-Reply-To: <8CBF670FC352747-2ACC-10E5C@webmail-m043.sysops.aol.com> Message-ID: <713670.9180.qm@web46105.mail.sp1.yahoo.com> I have never heard of an EM lab making their own resin for embedding. We almost all buy the resin kits or the separate components to make either a Epon 812 equivalent or Spurrs. The amount of time it takes to process is usually tissue dependent. On average, it takes one day to process (if the tissue is 1mm cubed or less) and 2 days in the oven for the resin to polymerize. You can speed things up if you microwave process. If you have any questions, please feel free to contact me. Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Sat, 8/29/09, histopatty@aol.com wrote: > From: histopatty@aol.com > Subject: [Histonet] EM processing > To: histonet@lists.utsouthwestern.edu > Date: Saturday, August 29, 2009, 3:36 AM > Does your lab make their own plastic > resin to process and embed specimens or do you buy it > commercially? > What is the average time it take to process a specimen? > > Thanks in advance to all who respond. > P.Eneff > OU Medical Center > Oklahoma City, OK > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shive003 <@t> umn.edu Sat Aug 29 16:35:36 2009 From: shive003 <@t> umn.edu (shive003@umn.edu) Date: Sat Aug 29 16:35:39 2009 Subject: [Histonet] COX-2 In-Reply-To: <4A98FF77.7400.0077.1@harthosp.org> References: <4A97EB9C020000EE0002BFF1@smtp-gw.hurleymc.com> <4A98FF77.7400.0077.1@harthosp.org> Message-ID: In the meantime, you can google "COX-2 aspirin colorectal" and get caught up on the various articles written on the topic in the recent past. Jan Shivers UMN VDL On Aug 29 2009, Richard Cartun wrote: >It was in "USA Today", but will be published in this week's issue of JAMA. > >RWC > >Richard W. Cartun, Ph.D. >Director, Histology & Immunopathology >Director, Biospecimen Collection Programs >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 545-1596 >(860) 545-0174 Fax > >>>> "Lynette Pavelich" 8/28/2009 2:37 PM >>> >Could you share the source of the study? > >thank you, >Lynette > >Lynette Pavelich, HT(ASCP) >Histology Supervisor >MSH Competency Coordinator >Hurley Medical Center >One Hurley Plaza >Flint, MI 48503 >email: Lpaveli1@hurleymc.com >ph: 810-257-9948 >fax: 810-762-7082 >>>> "Richard Cartun" 08/28/09 2:32 PM >>> >There was a recent study that showed "aspirin" affected the growth of >one type of colorectal cancer; that which overexpresses COX-2. If this >holds up, get ready to start doing a lot of COX-2 IHC. > >Does anyone have a good mAb to COX-2 that works on formalin-fixed, >paraffin-embedded tissue that they would recommend? Thank you. > >Richard > >Richard W. Cartun, Ph.D. >Director, Histology & Immunopathology >Director, Biospecimen Collection Programs >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 545-1596 >(860) 545-0174 Fax > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWeems <@t> sjha.org Sat Aug 29 17:31:52 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Sat Aug 29 17:35:46 2009 Subject: [Histonet] h.pylori silver staining References: <652238.69300.qm@web35605.mail.mud.yahoo.com><868447.75422.qm@web65712.mail.ac4.yahoo.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA53C2FE0@ITSSSXM01V6.one.ads.che.org> And to that we add the Alcian blue, H&E modification portion of the Genta stain procedure. Works perfectly. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of WILLIAM DESALVO Sent: Sat 8/29/2009 2:38 PM To: rjbuesa@yahoo.com; histonet; sabeti_shahram@yahoo.com Subject: RE: [Histonet] h.pylori silver staining A Warthin-Starry procedure works great. The kit and procedure on the DAKO Artisan is fast, reliable and provides good results. William DeSalvo, B.S., HTL(ASCP) > Date: Sat, 29 Aug 2009 07:32:10 -0700 > From: rjbuesa@yahoo.com > To: histonet@lists.utsouthwestern.edu; sabeti_shahram@yahoo.com > Subject: Re: [Histonet] h.pylori silver staining > CC: > > Shahram: > Under separate cover I am sending you the procedure. > Ren? J. > > --- On Sat, 8/29/09, Shahram Sabeti wrote: > > > From: Shahram Sabeti > Subject: [Histonet] h.pylori silver staining > To: histonet@lists.utsouthwestern.edu > Date: Saturday, August 29, 2009, 3:48 AM > > > hello dear colleagues > i have heard of a new modified silver staining method for H.pylori.do you know anything about it and it's methology.please inform me if possible. > yours > sabeti > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From thomas.crowell <@t> novartis.com Sun Aug 30 12:09:34 2009 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Sun Aug 30 12:09:41 2009 Subject: [Histonet] Thomas Crowell is out of the office. Message-ID: I will be out of the office starting 08/30/2009 and will not return until 09/08/2009. Please contact Humphrey Gardner at 617-871-3590 if you have any questions regarding clinical trial samples. From o.isaac24 <@t> yahoo.com Sun Aug 30 14:10:24 2009 From: o.isaac24 <@t> yahoo.com (Isaac O) Date: Sun Aug 30 14:10:28 2009 Subject: [Histonet] IHC/HISTOTECH POSITION WANTED Message-ID: <651020.38739.qm@web111618.mail.gq1.yahoo.com> Hi, ?? I am looking for a new Histotech/IHC position. I am HTL(ASCP) certified. I am open to relocation. ?Isaac. BS, HT, HTL(ASCP) From lelmgren <@t> sunriselab.com Sun Aug 30 15:34:03 2009 From: lelmgren <@t> sunriselab.com (Laurie Elmgren) Date: Sun Aug 30 15:34:10 2009 Subject: [Histonet] Thermo Slide Mate Printers Message-ID: <4A672C6AE0402D4A89ECE29E8A4B47E3029E9522@MailPDC.sunriselab.com> Hi Lisa, I am currently using the Slide Mate printers in Histology. My Personal experience using the instrument has been very good. The type of slides used is critical, as well as handling them so that absolutely no moisture is transferred to them. We tried several types of slides, and found by trial and error that some come prestuck (together) and some do not, even same brands, different styles. The frosted vs painted labeling area is really a non-issue. For the charged slides we prefer a brand that was not recommended by Thermo (because of the texture on the paint). We simply change the darkness setting to adjust for that. Rarely the print will flake off if frosted slides are used and the print is touched when it is wet with water. I have also found that using a dry clean brush to dust the inside after every load is critical in avoiding jams. Aide from that, we have had no problems. In fact, we have been very shorthanded, and without them our work would never have gotten out on time and accurately labeled. We work scanning one block, cut the block and by the time the ribbon is ready to be picked up, the slide is printed. This avoids any mismatch of slide/ block. When used in conjunction with the printmate cassette printer, they system is invaluable to me. Laurie Elmgren Histology Supervisor "This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it." From katherine-walters <@t> uiowa.edu Mon Aug 31 08:04:06 2009 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Mon Aug 31 08:04:16 2009 Subject: [Histonet] EM processing In-Reply-To: <8CBF670FC352747-2ACC-10E5C@webmail-m043.sysops.aol.com> References: <8CBF670FC352747-2ACC-10E5C@webmail-m043.sysops.aol.com> Message-ID: We make our own from commercially available components. It takes on an average 3-4 days from fixative to cured block. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histopatty@aol.com Sent: Friday, August 28, 2009 10:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EM processing Does your lab make their own plastic resin to process and embed specimens or do you buy it commercially? What is the average time it take to process a specimen? Thanks in advance to all who respond. P.Eneff OU Medical Center Oklahoma City, OK _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon Aug 31 08:18:39 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Aug 31 08:18:51 2009 Subject: [Histonet] COX-2 In-Reply-To: <4A97EA5F.7400.0077.1@harthosp.org> Message-ID: <206B327B0E074CDCA7255C3CAE3B67B5@lurie.northwestern.edu> Richard, We use the COX2 for Cayman chemicals and have never had any issues with it. It stores at -20 once you rehydrate and aliquot. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, August 28, 2009 1:32 PM To: Histonet Subject: [Histonet] COX-2 There was a recent study that showed "aspirin" affected the growth of one type of colorectal cancer; that which overexpresses COX-2. If this holds up, get ready to start doing a lot of COX-2 IHC. Does anyone have a good mAb to COX-2 that works on formalin-fixed, paraffin-embedded tissue that they would recommend? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Aug 31 10:27:30 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Aug 31 10:27:32 2009 Subject: [Histonet] RELIA Histology Careers Update. Summer is almost over! Can you Believe it? Message-ID: Hi Histonetters! It is hard to believe the summer is almost over. The kids are going back to school and Labor Day is next week!! Before we know it will be Halloween, Thanksgiving, Christmas and even New Years Day!! Let?s Get The Ball Rolling!!! Looking for a new job in the Fall? Planning a job change after the holidays? Considering making a move in 2010? Are you a histo tech looking for a something better? Are you a new or recent graduate of a histology school? Are you a traveler considering transitioning into a permanent position? Are you a Histo Tech looking for an opportunity to advance into management? Let?s Get The Ball Rolling!!! I can put RELIA?s 5 Point Career Mapping Strategy to Work for You. Here?s how it works: R*E*L*I*A* ? *Resume Fine Tuning ? *Economic Analysis ?*Laser in on your dream job ? *Interview Coaching ? *Arrangements and Timeframes Customized to you Convenience All you have to do is contact me at relia1@earthlink.net or at 866-607-3542 my office is open M-F 7am-8pm EST or by appointment. If you know of anyone else who might be interested in hearing about RELIA?s Career Mapping Strategy or would like to subscribe to RELIA?s Histology Careers Bulletin please feel free to pass this along to them. Here is some information about my current openings: Histology Managers/Supervisors needed in: OR-Portland-Pathology Manager WA-Spokane-Histology Supervisor-Hospital WA-Spokane-Histology Supervisor -Private Pathology Lab TX- San Antonio-Production Shift Supervisor-Night Shift PA ? Pittsburgh ?Cytology Supervisor (if you know somebody) Histotechnicians/Histotechnologists needed in: PA- Pittsburgh, Dayshift HT or HTL required. NY-Orange/Rockand County NYS license req NY-Upstate NY NYS license req NY-NYC night shift NYS license req CA - Los Angeles, Histotechnician/Histotechnologist ASCP cert req MA ? North of Boston 2nd shift position Histotechnician/Histotechnologist FL- Largo part time FL lic req Remember it never hurts to look!!! Thanks, Pam 877-60 RELIA (877-607-3542) I know there are a lot of recruiters out there right now contacting you and your friends. RELIA is the only nationwide recruiting firm specializing in the permanent placement of histology professionals. Remember here at RELIA we work as your confidential advocate to help you make the move that is right for you when the time is right for you. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Phone: (877)607-3542 Mobile: (407)353-5070 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia www.twitter.com/pamatrelia From Janice.Mahoney <@t> alegent.org Mon Aug 31 11:15:38 2009 From: Janice.Mahoney <@t> alegent.org (Mahoney,Janice A) Date: Mon Aug 31 11:15:48 2009 Subject: [Histonet] Gloves Message-ID: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE2E@EXCHMBC2.ad.ah.local> I'd like some advice about gloves. What brand/vendor are people using that are approved for use with xylene? Thanks, Jan Mahoney Omaha ________________________________ Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From leiker <@t> buffalo.edu Mon Aug 31 11:43:38 2009 From: leiker <@t> buffalo.edu (Merced M Leiker) Date: Mon Aug 31 11:43:46 2009 Subject: [Histonet] Gloves In-Reply-To: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE2E@EXCHMBC2.ad.ah.local> References: <8F0EE4144E8E2F4CA1F6B051A2E5BFEE2BDE2E@EXCHMBC2.ad.ah.local> Message-ID: I would like to know the answer to this one too. I've been using nitrile gloves, which are rated as having moderate protection against xylene (they slow down how quickly xylene goes through them?) The brand I use is what's in our stockroom: Kimberly Clark and they come in a pretty purple. :-) --On Monday, August 31, 2009 11:15 AM -0500 "Mahoney,Janice A" wrote: > I'd like some advice about gloves. What brand/vendor are people using > that are approved for use with xylene? Thanks, > Jan Mahoney > Omaha > > ________________________________ > Sponsored by Catholic Health Initiatives and Immanuel Health Systems, > Alegent Health is faithful to the healing ministry of Jesus Christ, > providing high quality care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, > is confidential and private and intended only for the use of the > addressees. Unauthorized use, disclosure, distribution or copying is > strictly prohibited and may be unlawful. If you received this > communication in error, please inform us of the erroneous delivery by > return e-mail message from your computer. Additionally, although all > attachments have been scanned at the source for viruses, the recipient > should check any attachments for the presence of viruses before opening. > Alegent Health accepts no liability for any damage caused by any virus > transmitted by this e-mail. Thank you for your cooperation. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA leiker@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From carl.hobbs <@t> kcl.ac.uk Mon Aug 31 12:25:27 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Mon Aug 31 12:27:14 2009 Subject: [Histonet] COX-2 Message-ID: <11D9615B89C10747B1C985966A63D7CA2991742A14@KCL-MAIL04.kclad.ds.kcl.ac.uk> Why a mouse Ab? I have two anti-Cox-2 rabbit polys that seem to co-localise. From Jane.Moose <@t> NewberryHospital.net Mon Aug 31 13:34:01 2009 From: Jane.Moose <@t> NewberryHospital.net (Jane C. Moose) Date: Mon Aug 31 13:35:48 2009 Subject: [Histonet] FNA SLIDES Message-ID: <8BB5FC36DDA373488E60177757BBD6985FE011@ncmhexchbe01.NewberryHospital.net> A question has arisen for us- How many slides do you (should you) make per pass for pathologist for adequacy and/or diagnosis? What about CT guided biopsies of liver, lung, "masses" etc. Thanks in advance for your input. Jane Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 From sfeher <@t> CMC-NH.ORG Mon Aug 31 14:03:24 2009 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Mon Aug 31 14:03:31 2009 Subject: [Histonet] FNA SLIDES In-Reply-To: <8BB5FC36DDA373488E60177757BBD6985FE011@ncmhexchbe01.NewberryHospital.net> References: <8BB5FC36DDA373488E60177757BBD6985FE011@ncmhexchbe01.NewberryHospital.net> Message-ID: <73A7ED895EE0C24D9267ED814911DF190FDB1DF3@exchange.cmc-nh.org> A lot depends on the pathologist and the nature of the specimen. For CT guided biopsies where you get a core and do touch preps. One slide per pass should be sufficient. For FNA's where you get fluid I make two slides per staining medium (some pathologists want Toluene Blue and Diff Quick. Some want a Diff Quik and a Pap Stain). The remainder of the fluid should be put into CytoLyt or other suitable fluid for cell block and/or Liquid Based Cytology processing. It is possible to do a rapid Pap that while not nearly as quick as the Diff Quick is still doable. The bottom line is that it is up to the pathologist that has to look at them. Hope this helps. Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jane C. Moose Sent: Monday, August 31, 2009 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FNA SLIDES A question has arisen for us- How many slides do you (should you) make per pass for pathologist for adequacy and/or diagnosis? What about CT guided biopsies of liver, lung, "masses" etc. Thanks in advance for your input. Jane Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sandy.Schmitz <@t> leica-microsystems.com Mon Aug 31 16:01:47 2009 From: Sandy.Schmitz <@t> leica-microsystems.com (Sandy.Schmitz@leica-microsystems.com) Date: Mon Aug 31 16:01:59 2009 Subject: [Histonet] Schmitz, Sandy is out of the office. Message-ID: I will be out of the office starting 08/31/2009 and will not return until 09/03/2009. I will reply to your message upon my return. Thank you. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From natecrmr <@t> gmail.com Mon Aug 31 16:02:20 2009 From: natecrmr <@t> gmail.com (Nathan Cramer) Date: Mon Aug 31 16:02:30 2009 Subject: [Histonet] uneven alternating sections on cryostat Message-ID: <4A9C3A5C.1060605@gmail.com> Thank you very much to everyone who has provided suggestions to help me fix my uneven section problem. I am preparing some more samples (spinal cord) at the moment and will see how things go later this week. I've summarized the suggestions below (in case someone else has the problem and finds this thread). 1. Check tightness of blade holder and be sure it is even in tightness on both sides. 2. Check tightness of screws/bolts pretty much everywhere including those that hold the cryostat to the cabinet and the chuck holder in place. 3. Check/adjust the blade angle. If it is too vertical, it can start cutting the front but compress the back of the specimen causing uneven sections. 4. There may be an issue with the cryostat head retracting too slowly. This could be due to my cutting too quickly or the need for a defrost/cleaning (by someone who knows what they are doing). 5. The orientation of the sample can influence how well the slices turn out. Changing the orientation may improve things. 6. Don't let the sample protrude too far away from the chuck. If I find the smoking gun, I'll post an update... Best wishes Nate From AnthonyH <@t> chw.edu.au Mon Aug 31 17:53:59 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Aug 31 17:54:05 2009 Subject: [Histonet] FNA SLIDES In-Reply-To: <8BB5FC36DDA373488E60177757BBD6985FE011@ncmhexchbe01.NewberryHospital.net> Message-ID: We make two slides. One air dried DQ for immediate assessment, second alcohol fixed for PAP in the lab. The "spreader" we either stain with Giemsa or rehydrate (using saline) and stain with PAP in lab. Triage as follows: If inflammatory, second pass for microbiology. If malignant, if required, then second pass for cell block. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jane C. Moose Sent: Tuesday, 1 September 2009 4:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FNA SLIDES A question has arisen for us- How many slides do you (should you) make per pass for pathologist for adequacy and/or diagnosis? What about CT guided biopsies of liver, lung, "masses" etc. Thanks in advance for your input. Jane Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From eridana <@t> cox.net Mon Aug 31 20:38:16 2009 From: eridana <@t> cox.net (Eridana) Date: Mon Aug 31 20:38:19 2009 Subject: [Histonet] San Diego Chapter meeting In-Reply-To: <20090831170224.EYKJ14906.fed1rmmtai114.cox.net@fed1rmimpi02.cox.net> Message-ID: <20090831213816.B7OIB.97277.imail@fed1rmwml42> The San Diego chapter will be having our next meeting on Sept 10 from 4:30 to 7 at Pfizer. The topic will be on laboratory safety and hopefull we will come up with more topics for future meetings. There will be a light dinner and everyone is welcome but please let us know you are planning to attend. Please contact me for further information or to RSVP Thanks Donna Harclerode eridana@cox.net