From ree3 <@t> leicester.ac.uk Wed Apr 1 03:50:51 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed Apr 1 03:51:07 2009 Subject: [Histonet] G 20 MEETING NEWS Message-ID: <7722595275A4DD4FA225B92CDBF174A17457783C7D@EXC-MBX3.cfs.le.ac.uk> Apparently Obama and Brown, with the reluctant compliance of Germany and China are aiming to rationalise the pay and conditions of laboratory workers worldwide, and hopefully if they succeed the histotechs in the U.S.A. will one be the major beneficiaries!, so fingers xxxed everyone. From lblazek <@t> digestivespecialists.com Wed Apr 1 06:22:49 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Apr 1 06:18:12 2009 Subject: [Histonet] Leica Bond Max - any opinions? In-Reply-To: <001101c9b262$40984570$5c237d80@DFS66DD1> References: <001101c9b262$40984570$5c237d80@DFS66DD1> Message-ID: <5A2BD13465E061429D6455C8D6B40E3908778D151F@IBMB7Exchange.digestivespecialists.com> I would really suggest you look closely at the Intellipath from BioCare. They are a truly open system. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle MacVeigh-Aloni Sent: Tuesday, March 31, 2009 8:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond Max - any opinions? Hi all, I am researching the Leica Bond Max immunostainer and I like its features as advertised. The advertisement however states that the system is completely open, but after discussing it in length with the Leica rep I am finding that this is actually not so... We are a research core and work with mice and rats. Most of the pre-made antibody are designed towards human tests, so we won't use them. Then we would still be stuck with lots of money for consumables (as I understood all sold as kits with the antibodies included). We won't be using any of the antibodies, only the rest of the solutions... Is there a way out of this trap? Is there something that I didn't understand? My question is: Does anybody use Leica Bond as an open system and use other companies antibody, buffers and antigen retrieval protocols? If you do, what have you done so far? (Would you share your protocols?) We like the BioCare products and our helpful rep :-) Did anybody purchase the Research dongle? Do we really need it? How did it help you improve your applications? I was told that depending on what I want unlocked, we would have to spend $14000 to $27000 extra for it. OUCH!!! This is not a hospital where we can charge the insurance or patients for this... I need this info ASAP, because we have to make our decision in a day and there is no time to demo the unit (nor anything else). I understand that it is great for pathology, but how about animal research? It costs A LOT!!! We better know what are we getting ourselves into. Please help! Any advise and suggestions that I can get would be highly appreciated. Michelle Mac Veigh Aloni MS, HTL USC School of Medicine Department of GI and Liver Los Angeles, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Wed Apr 1 07:28:42 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Apr 1 07:29:10 2009 Subject: [Histonet] Leica Bond Max - any opinions? In-Reply-To: <5A2BD13465E061429D6455C8D6B40E3908778D151F@IBMB7Exchange.digestivespecialists.com> Message-ID: I agree with Linda. I researched 5 different automated IHC stainers to be used for toxicology research applications, and found the intelliPATH to be the best solution for our needs. Biocare, as well, has a vast array of anti rat and mouse primary antibodies and detection systems. Additionally, the cost for the intelliPATH is quite reasonable, compared to the other platforms. Jackie O' "Blazek, Linda" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/01/2009 06:22 AM To 'Michelle MacVeigh-Aloni' , "histonet@lists.utsouthwestern.edu" cc Subject RE: [Histonet] Leica Bond Max - any opinions? I would really suggest you look closely at the Intellipath from BioCare. They are a truly open system. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle MacVeigh-Aloni Sent: Tuesday, March 31, 2009 8:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond Max - any opinions? Hi all, I am researching the Leica Bond Max immunostainer and I like its features as advertised. The advertisement however states that the system is completely open, but after discussing it in length with the Leica rep I am finding that this is actually not so... We are a research core and work with mice and rats. Most of the pre-made antibody are designed towards human tests, so we won't use them. Then we would still be stuck with lots of money for consumables (as I understood all sold as kits with the antibodies included). We won't be using any of the antibodies, only the rest of the solutions... Is there a way out of this trap? Is there something that I didn't understand? My question is: Does anybody use Leica Bond as an open system and use other companies antibody, buffers and antigen retrieval protocols? If you do, what have you done so far? (Would you share your protocols?) We like the BioCare products and our helpful rep :-) Did anybody purchase the Research dongle? Do we really need it? How did it help you improve your applications? I was told that depending on what I want unlocked, we would have to spend $14000 to $27000 extra for it. OUCH!!! This is not a hospital where we can charge the insurance or patients for this... I need this info ASAP, because we have to make our decision in a day and there is no time to demo the unit (nor anything else). I understand that it is great for pathology, but how about animal research? It costs A LOT!!! We better know what are we getting ourselves into. Please help! Any advise and suggestions that I can get would be highly appreciated. Michelle Mac Veigh Aloni MS, HTL USC School of Medicine Department of GI and Liver Los Angeles, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dwitt <@t> sebh.org Wed Apr 1 07:36:48 2009 From: dwitt <@t> sebh.org (Witt, Dan) Date: Wed Apr 1 07:37:18 2009 Subject: [Histonet] (no subject) Message-ID: We currently have a full time job opening at our lab. We are hospital based and are located 15 miles east of St. Louis, mo in in Belleville, IL. If interested contact Dan Witt at dwitt@sebh.org. From rjbuesa <@t> yahoo.com Wed Apr 1 07:41:06 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 1 07:41:09 2009 Subject: [Histonet] Online SOP's In-Reply-To: <3EEADAB0392281448505732B7323D9A4047676@MSGEBE37.mfad.mfroot.org> Message-ID: <572708.32423.qm@web65710.mail.ac4.yahoo.com> Good question, to which you have to add: how ALL the personnel supposed to use the SOP sign-off? It does not take so much space after all, so keep your SOP hard copy and make it sign by all. Ren? J. --- On Tue, 3/31/09, Bauer, Karen L. wrote: From: Bauer, Karen L. Subject: [Histonet] Online SOP's To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 31, 2009, 2:29 PM Hello, Does anyone have only procedures online? All of ours are online, but we still keep a paper copy of each in a manual in the lab. I would like to get rid of the manual, but then how do you record pathologist sign-off on all new or revised procedures? I'm curious to hear what other labs are doing. Thanks much, Karen Karen L. Bauer HT(ASCP), BS Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.karen@mayo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Apr 1 07:43:04 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 1 07:43:09 2009 Subject: [Histonet] Histology Training In-Reply-To: <42DFE1A029181B4B8CCBA7261B52D765EA51DC@suffieldex01.suffield.drdc-rddc.gc.ca> Message-ID: <680853.65329.qm@web65711.mail.ac4.yahoo.com> And what kind of a training are you going to receive in 1-2 weeks? Very incomplete I suppose! Ren? J. --- On Tue, 3/31/09, Poole, Kimberly wrote: From: Poole, Kimberly Subject: [Histonet] Histology Training To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 31, 2009, 3:38 PM Hi, Does anyone know of a place that I can get histology training? I am specifically looking for a place in the US or Canada to go to for a couple of weeks. I am not able to take courses that are 1 to 2 years long. Thanks in advance!! Kimberly Poole B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield Medicine Hat, AB, Canada T1A 8K6 kimberly.poole@drdc-rddc.gc.ca Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 Government of Canada | Gouvernement du Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Wed Apr 1 07:44:59 2009 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Wed Apr 1 07:45:02 2009 Subject: [Histonet] Histology Training In-Reply-To: <680853.65329.qm@web65711.mail.ac4.yahoo.com> References: <680853.65329.qm@web65711.mail.ac4.yahoo.com> Message-ID: <202583.57065.qm@web53607.mail.re2.yahoo.com> Is that an April Fools joke? ? Larry A. Woody Seattle, Wa. ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; "Poole, Kimberly" Sent: Wednesday, April 1, 2009 5:43:04 AM Subject: Re: [Histonet] Histology Training And what kind of a training are you going to receive in 1-2 weeks? Very incomplete I suppose! Ren? J. --- On Tue, 3/31/09, Poole, Kimberly wrote: From: Poole, Kimberly Subject: [Histonet] Histology Training To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 31, 2009, 3:38 PM Hi, Does anyone know of a place that I can get histology training? I am specifically looking for a place in the US or Canada to go to for a couple of weeks. I am not able to take courses that are 1 to 2 years long. Thanks in advance!! Kimberly Poole B.Sc Casualty Management Section | Section de la gestion des bless?s Defence Research and Development Canada Suffield | Recherche et d?veloppement pour la d?fense Canada Suffield Medicine Hat, AB, Canada T1A 8K6 kimberly.poole@drdc-rddc.gc.ca Telephone | T?l?phone 403-544-5347 / Facsimile | T?l?copieur 403-544-4714 Government of Canada | Gouvernement du Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Apr 1 07:45:39 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 1 07:45:43 2009 Subject: [Histonet] Effects of Cytocool on IHC In-Reply-To: <5998F3BDFF7AAC4091C7AE93A7A1A5892CDF3F@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <33709.37078.qm@web65709.mail.ac4.yahoo.com> That is another "urban legend", spray if you need, no effect on IHC (or anything else) whatsoever. Ren? J. --- On Tue, 3/31/09, Sebree Linda A wrote: From: Sebree Linda A Subject: RE: [Histonet] Effects of Cytocool on IHC To: "Leslie McCormack" , histonet@lists.utsouthwestern.edu Date: Tuesday, March 31, 2009, 5:04 PM I have heard not to use freezing spray on blocks/sections that are destined for IHC but I never heard the reasoning behind this warning. I've always steered clear of it when cutting for IHC and instead will bury my block in the frost of our freezer until its good and cold. Works fine. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leslie McCormack Sent: Tuesday, March 31, 2009 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Effects of Cytocool on IHC In our lab many of the histotechs use Cytocool when cutting paraffin blocks. Has anyone heard of this having a negative effect on IHC? Leslie McCormack Baptist Medical Center Jacksonville, FL 32207 _________________________________________________________________ Express your personality in color! Preview and select themes for Hotmail(r). http://www.windowslive-hotmail.com/LearnMore/personalize.aspx?ocid=TXT_M SGTX_WL_HM_express_032009#colortheme____________________________________ ___________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Wed Apr 1 07:55:27 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Apr 1 07:55:41 2009 Subject: [Histonet] Calcium salts In-Reply-To: Message-ID: <6105C06A5DD74E9BA68C91A15F66B72A@lurie.northwestern.edu> Sodium thiosulfate removes excess silver and has been used in the Von Kossa technique for years as well as most,if not all silver stains we do. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Tuesday, March 31, 2009 5:27 PM To: Anthony Reilly; histonet Subject: RE: [Histonet] Calcium salts Tony, I have been lead to believe that after von Kossa staining, Silver carbonate dissolves in sodium thiosulphate and cannot be demonstrated with von Kossa's technique. Though I have not confirmed this Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony Reilly Sent: Tuesday, 31 March 2009 4:33 PM To: histonet Subject: [Histonet] Calcium salts Hello I am trying to differentiate Calcium Phosphate from Calcium Carbonate which both stain with the von Kossa method. Is there a pretreatment for which only one is soluble that could be used to differentiate the 2 components. thanks Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au ************************************************************************ ******** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ************************************************************************ ********** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dolores_Fischer <@t> baxter.com Wed Apr 1 08:00:52 2009 From: Dolores_Fischer <@t> baxter.com (Dolores_Fischer@baxter.com) Date: Wed Apr 1 08:01:00 2009 Subject: [Histonet] Leica Bond Max - any opinions? In-Reply-To: <001101c9b262$40984570$5c237d80@DFS66DD1> Message-ID: Michelle, We also only work with animal tissue. Have you considered researching Biocare's Intellipath immunostainer? We also love their products and this is an open system. I am not the one who made the purchase decision or used both of the stainers, but, although the Leica immunostainer was also well liked, for our purposes (animal tissue), the Intellipath was chosen. Dolroes The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From bernietaupin <@t> ymail.com Wed Apr 1 08:13:56 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 1 08:14:01 2009 Subject: [Histonet] onalighternote In-Reply-To: <408230.81711.qm@web46106.mail.sp1.yahoo.com> References: <408230.81711.qm@web46106.mail.sp1.yahoo.com> Message-ID: <241532.54644.qm@web43501.mail.sp1.yahoo.com> I don't know what makes some of you people persist in making fun of a complete stranger's name, but I'll have you know I was born before the person you are talking about. ________________________________ From: Va Paula Sicurello To: Jeanine (CDC/CCID/NCZVED)Bartlett ; R.E.Edwards ; histonet@lists.utsouthwestern.edu; Bernie Taupin Sent: Tuesday, March 31, 2009 7:01:14 PM Subject: Re: [Histonet] onalighternote Hey, we can't help it if your parents were big fans of his. He is a great song writer. ;-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Tue, 3/31/09, Bernie Taupin wrote: > From: Bernie Taupin > Subject: Re: [Histonet] onalighternote > To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , "Edwards, R.E." , histonet@lists.utsouthwestern.edu > Date: Tuesday, March 31, 2009, 4:52 PM > Yeah, hilarious.. I swear, I haven't > heart THAT one before > > > > > ________________________________ > From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" > To: "Edwards, R.E." ; > histonet@lists.utsouthwestern.edu > Sent: Tuesday, March 31, 2009 10:18:36 AM > Subject: RE: [Histonet] onalighternote > > I wanted to ask how Elton was but thought that was a bit > too easy....... > > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Edwards, > R.E. > Sent: Tuesday, March 31, 2009 10:16 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] onalighternote > > > I have just had eeees from Bernie > Taupin and Paul Schofield, is > this a record??. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Robyn > Vazquez > Sent: 31 March 2009 15:08 > To: Bernie Taupin; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Ford Royer > > I think Ford gets the message...GET ON WITH IT! > Antibody talk anyone? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Bernie > Taupin > Sent: Tuesday, March 31, 2009 6:50 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Ford Royer > > > I can personally attest that Ford must have been > having a VERY bad day > indeed. > > Although I can empathize that this might be the case, I do > find it > curious that we've heard nothing from Mr. Royer since his > little > outburst. > > I, for one, wouldn't mind an apology to the list for your > antisocial > behaviour on it, Mr. Royer. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bernietaupin <@t> ymail.com Wed Apr 1 08:16:55 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 1 08:16:59 2009 Subject: [Histonet] AO Blade holder In-Reply-To: <91692681-9B86-43BD-B875-ECD59D71F54B@kent.edu> References: <91692681-9B86-43BD-B875-ECD59D71F54B@kent.edu> Message-ID: <400079.63960.qm@web43507.mail.sp1.yahoo.com> Am I getting multiple mails or is it simply that David Waugh doesn't know how to use a listserv? ________________________________ From: David Waugh To: histonet@lists.utsouthwestern.edu Sent: Tuesday, March 31, 2009 3:08:08 PM Subject: [Histonet] AO Blade holder > I have a question regarding a AO disposable blade holder (Cat# 824). This link should show a picture of a similar one www.usedmicroscopes.com/images/AO820Microtome.jpg > > The blade is tightened with a lever on the right that rotates a d-shaped cam. Two screws are used the set the tension that the cam places on the blade, once set, the cam will tighten and loosen the blade. The back of the holder is concave, and the front part convex, so that when tightened, the blade will bow. I have not been able to find any documentation on how tight the blade should be. It sounds like most holders have flat surfaces that clamp the blade, which I would think would have very different tightness requirements. Any ideas from someone who uses this type of holder on how the two screws should be set? Thanks, David > > Kent State University > Department of Geology > Kent, Ohio 44242 > dwaugh@kent.edu > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pjfnefro <@t> duke.edu Wed Apr 1 08:20:30 2009 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Wed Apr 1 08:21:00 2009 Subject: [Histonet] onalighternote Message-ID: <5EF4C8CD-A3C4-4310-9684-2643254960B2@duke.edu> Then maybe we're really making fun of the "other" Bernie; we just didn't know it. :-) > I don't know what makes some of you people persist in making fun of > a complete stranger's name, but I'll have you know I was born before > the person you are talking about. > > > > > ________________________________ > From: Va Paula Sicurello > To: Jeanine (CDC/CCID/NCZVED)Bartlett ; R.E.Edwards >;histonet@lists.utsouthwestern.edu; Bernie Taupin > > Sent: Tuesday, March 31, 2009 7:01:14 PM > Subject: Re: [Histonet] onalighternote > > > Hey, we can't help it if your parents were big fans of his. He is a > great song writer. > > ;-) > > > Paula Sicurello > VA Medical Center San Diego > Veterans Medical Research Foundation (VMRF) > Core Research Imaging Center > 3350 La Jolla Village Dr., MC151 > San Diego, CA 92161 > 858-552-8585 x2397 > > > --- On Tue, 3/31/09, Bernie Taupin wrote: > >> From: Bernie Taupin >> Subject: Re: [Histonet] onalighternote >> To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , "Edwards, >> R.E." ,histonet@lists.utsouthwestern.edu >> Date: Tuesday, March 31, 2009, 4:52 PM >> Yeah, hilarious.. I swear, I haven't >> heart THAT one before >> >> >> >> >> ________________________________ >> From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" >> To: "Edwards, R.E." ; >> histonet@lists.utsouthwestern.edu >> Sent: Tuesday, March 31, 2009 10:18:36 AM >> Subject: RE: [Histonet] onalighternote >> >> I wanted to ask how Elton was but thought that was a bit >> too easy....... >> >> >> >> Jeanine Bartlett >> Infectious Diseases Pathology Branch >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Edwards, >> R.E. >> Sent: Tuesday, March 31, 2009 10:16 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] onalighternote >> >> >> I have just had eeees from Bernie >> Taupin and Paul Schofield, is >> this a record??. >> From bernietaupin <@t> ymail.com Wed Apr 1 08:50:34 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 1 08:50:46 2009 Subject: [Histonet] onalighternote In-Reply-To: <5EF4C8CD-A3C4-4310-9684-2643254960B2@duke.edu> References: <5EF4C8CD-A3C4-4310-9684-2643254960B2@duke.edu> Message-ID: <615917.22149.qm@web43512.mail.sp1.yahoo.com> > Then maybe we're really making fun of the "other" Bernie; we just didn't know it. :-) Perhaps you should get back to your histology and just plain stop making fun of anyone, for any reason. ________________________________ From: Pat Flannery To: histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 9:20:30 AM Subject: Re: [Histonet] onalighternote Then maybe we're really making fun of the "other" Bernie; we just didn't know it. :-) > I don't know what makes some of you people persist in making fun of a complete stranger's name, but I'll have you know I was born before the person you are talking about. > > > > > ________________________________ > From: Va Paula Sicurello > To: Jeanine (CDC/CCID/NCZVED)Bartlett ; R.E.Edwards ;histonet@lists.utsouthwestern.edu; Bernie Taupin > Sent: Tuesday, March 31, 2009 7:01:14 PM > Subject: Re: [Histonet] onalighternote > > > Hey, we can't help it if your parents were big fans of his. He is a great song writer. > > ;-) > > > Paula Sicurello > VA Medical Center San Diego > Veterans Medical Research Foundation (VMRF) > Core Research Imaging Center > 3350 La Jolla Village Dr., MC151 > San Diego, CA 92161 > 858-552-8585 x2397 > > > --- On Tue, 3/31/09, Bernie Taupin wrote: > >> From: Bernie Taupin >> Subject: Re: [Histonet] onalighternote >> To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , "Edwards, R.E." ,histonet@lists.utsouthwestern.edu >> Date: Tuesday, March 31, 2009, 4:52 PM >> Yeah, hilarious.. I swear, I haven't >> heart THAT one before >> >> >> >> >> ________________________________ >> From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" >> To: "Edwards, R.E." ; >> histonet@lists.utsouthwestern.edu >> Sent: Tuesday, March 31, 2009 10:18:36 AM >> Subject: RE: [Histonet] onalighternote >> >> I wanted to ask how Elton was but thought that was a bit >> too easy....... >> >> >> >> Jeanine Bartlett >> Infectious Diseases Pathology Branch >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Edwards, >> R.E. >> Sent: Tuesday, March 31, 2009 10:16 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] onalighternote >> >> >> I have just had eeees from Bernie >> Taupin and Paul Schofield, is >> this a record??. >> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Wed Apr 1 08:55:18 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 1 08:55:23 2009 Subject: [Histonet] onalighternote In-Reply-To: <7722595275A4DD4FA225B92CDBF174A17457783C75@EXC-MBX3.cfs.le.ac.uk> References: <930298.3241.qm@web43515.mail.sp1.yahoo.com> <2A582E8156B45F468A62D1F1D20AF083713FA3@EX-BE08.ohsu.edu> <7722595275A4DD4FA225B92CDBF174A17457783C75@EXC-MBX3.cfs.le.ac.uk> Message-ID: <259690.871.qm@web43515.mail.sp1.yahoo.com> > I have just had eeees from Bernie Taupin and Paul Schofield, is this a record?? I would try to reply to this, but I have no idea what "eeees" are. ________________________________ From: "Edwards, R.E." To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, March 31, 2009 10:15:51 AM Subject: [Histonet] onalighternote I have just had eeees from Bernie Taupin and Paul Schofield, is this a record??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer > I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Wed Apr 1 08:57:53 2009 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Wed Apr 1 08:58:02 2009 Subject: [Histonet] onalighternote In-Reply-To: <259690.871.qm@web43515.mail.sp1.yahoo.com> References: <930298.3241.qm@web43515.mail.sp1.yahoo.com><2A582E8156B45F468A62D1F1D20AF083713FA3@EX-BE08.ohsu.edu><7722595275A4DD4FA225B92CDBF174A17457783C75@EXC-MBX3.cfs.le.ac.uk> <259690.871.qm@web43515.mail.sp1.yahoo.com> Message-ID: <2A582E8156B45F468A62D1F1D20AF083714112@EX-BE08.ohsu.edu> Please stop replying and use your own advice. Histology talk anyone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 6:55 AM To: Edwards, R.E.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] onalighternote > I have just had eeees from Bernie Taupin and Paul Schofield, is this a record?? I would try to reply to this, but I have no idea what "eeees" are. ________________________________ From: "Edwards, R.E." To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, March 31, 2009 10:15:51 AM Subject: [Histonet] onalighternote I have just had eeees from Bernie Taupin and Paul Schofield, is this a record??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer > I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Wed Apr 1 08:53:57 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 1 09:00:41 2009 Subject: [Histonet] G 20 MEETING NEWS Message-ID: <84830.1474.qm@web43501.mail.sp1.yahoo.com> Please refrain from discussing politics on this list. Edwards, R.E. wrote: > Apparently Obama and Brown, with the reluctant compliance of Germany and > China are aiming to rationalise the pay and conditions of laboratory > workers worldwide, and hopefully if they succeed the histotechs in the > U.S.A. will one be the major beneficiaries!, so fingers xxxed everyone. From bernietaupin <@t> ymail.com Wed Apr 1 09:02:36 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 1 09:02:40 2009 Subject: [Histonet] onalighternote In-Reply-To: <2A582E8156B45F468A62D1F1D20AF083714112@EX-BE08.ohsu.edu> References: <930298.3241.qm@web43515.mail.sp1.yahoo.com><2A582E8156B45F468A62D1F1D20AF083713FA3@EX-BE08.ohsu.edu><7722595275A4DD4FA225B92CDBF174A17457783C75@EXC-MBX3.cfs.le.ac.uk> <259690.871.qm@web43515.mail.sp1.yahoo.com> <2A582E8156B45F468A62D1F1D20AF083714112@EX-BE08.ohsu.edu> Message-ID: <400628.17464.qm@web43507.mail.sp1.yahoo.com> > Please stop replying and use your own advice. Histology talk anyone? I'm curious- Why didn't you seem to feel this way about the first 3-5 replies that were sent out making fun of my name, completely devoid of "histology talk"? ________________________________ From: Robyn Vazquez To: Bernie Taupin ; "Edwards, R.E." ; histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 9:57:53 AM Subject: RE: [Histonet] onalighternote Please stop replying and use your own advice. Histology talk anyone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 6:55 AM To: Edwards, R.E.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] onalighternote > I have just had eeees from Bernie Taupin and Paul Schofield, is this a record?? I would try to reply to this, but I have no idea what "eeees" are. ________________________________ From: "Edwards, R.E." To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, March 31, 2009 10:15:51 AM Subject: [Histonet] onalighternote I have just had eeees from Bernie Taupin and Paul Schofield, is this a record??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer > I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Apr 1 09:04:51 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Apr 1 09:05:07 2009 Subject: [Histonet] onalighternote In-Reply-To: <615917.22149.qm@web43512.mail.sp1.yahoo.com> References: <5EF4C8CD-A3C4-4310-9684-2643254960B2@duke.edu> <615917.22149.qm@web43512.mail.sp1.yahoo.com> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF04C9@LTA3VS011.ees.hhs.gov> I enjoy the lighter side of things in my crazy day.............and the subject line warns us ahead of time.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 9:51 AM To: Pat Flannery Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] onalighternote > Then maybe we're really making fun of the "other" Bernie; we just > didn't know it. :-) Perhaps you should get back to your histology and just plain stop making fun of anyone, for any reason. ________________________________ From: Pat Flannery To: histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 9:20:30 AM Subject: Re: [Histonet] onalighternote Then maybe we're really making fun of the "other" Bernie; we just didn't know it. :-) > I don't know what makes some of you people persist in making fun of a complete stranger's name, but I'll have you know I was born before the person you are talking about. > > > > > ________________________________ > From: Va Paula Sicurello > To: Jeanine (CDC/CCID/NCZVED)Bartlett ; R.E.Edwards > ;histonet@lists.utsouthwestern.edu; Bernie > Taupin > Sent: Tuesday, March 31, 2009 7:01:14 PM > Subject: Re: [Histonet] onalighternote > > > Hey, we can't help it if your parents were big fans of his. He is a great song writer. > > ;-) > > > Paula Sicurello > VA Medical Center San Diego > Veterans Medical Research Foundation (VMRF) Core Research Imaging > Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 > 858-552-8585 x2397 > > > --- On Tue, 3/31/09, Bernie Taupin wrote: > >> From: Bernie Taupin >> Subject: Re: [Histonet] onalighternote >> To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , "Edwards, R.E." ,histonet@lists.utsouthwestern.edu >> Date: Tuesday, March 31, 2009, 4:52 PM >> Yeah, hilarious.. I swear, I haven't >> heart THAT one before >> >> >> >> >> ________________________________ >> From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" >> To: "Edwards, R.E." ; >> histonet@lists.utsouthwestern.edu >> Sent: Tuesday, March 31, 2009 10:18:36 AM >> Subject: RE: [Histonet] onalighternote >> >> I wanted to ask how Elton was but thought that was a bit >> too easy....... >> >> >> >> Jeanine Bartlett >> Infectious Diseases Pathology Branch >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Edwards, >> R.E. >> Sent: Tuesday, March 31, 2009 10:16 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] onalighternote >> >> >> I have just had eeees from Bernie >> Taupin and Paul Schofield, is >> this a record??. >> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Apr 1 09:05:09 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Apr 1 09:05:22 2009 Subject: [Histonet] onalighternote In-Reply-To: <400628.17464.qm@web43507.mail.sp1.yahoo.com> References: <930298.3241.qm@web43515.mail.sp1.yahoo.com><2A582E8156B45F468A62D1F1D20AF083713FA3@EX-BE08.ohsu.edu><7722595275A4DD4FA225B92CDBF174A17457783C75@EXC-MBX3.cfs.le.ac.uk> <259690.871.qm@web43515.mail.sp1.yahoo.com> <2A582E8156B45F468A62D1F1D20AF083714112@EX-BE08.ohsu.edu> <400628.17464.qm@web43507.mail.sp1.yahoo.com> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF04CA@LTA3VS011.ees.hhs.gov> Hey Bern, I'm with you! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 10:03 AM To: Robyn Vazquez; Edwards, R.E.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] onalighternote > Please stop replying and use your own advice. Histology talk anyone? I'm curious- Why didn't you seem to feel this way about the first 3-5 replies that were sent out making fun of my name, completely devoid of "histology talk"? ________________________________ From: Robyn Vazquez To: Bernie Taupin ; "Edwards, R.E." ; histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 9:57:53 AM Subject: RE: [Histonet] onalighternote Please stop replying and use your own advice. Histology talk anyone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 6:55 AM To: Edwards, R.E.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] onalighternote > I have just had eeees from Bernie Taupin and Paul Schofield, is this a record?? I would try to reply to this, but I have no idea what "eeees" are. ________________________________ From: "Edwards, R.E." To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, March 31, 2009 10:15:51 AM Subject: [Histonet] onalighternote I have just had eeees from Bernie Taupin and Paul Schofield, is this a record??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer > I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Wed Apr 1 09:11:29 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Apr 1 09:21:06 2009 Subject: [Histonet] G 20 MEETING NEWS In-Reply-To: <84830.1474.qm@web43501.mail.sp1.yahoo.com> References: <84830.1474.qm@web43501.mail.sp1.yahoo.com> Message-ID: <49D33DD1.2B7F.00C9.0@geisinger.edu> Yes please. There are plenty of blogs out there for this kind of thing. >>> Bernie Taupin 4/1/2009 9:53 AM >>> Please refrain from discussing politics on this list. Edwards, R.E. wrote: > Apparently Obama and Brown, with the reluctant compliance of Germany and > China are aiming to rationalise the pay and conditions of laboratory > workers worldwide, and hopefully if they succeed the histotechs in the > U.S.A. will one be the major beneficiaries!, so fingers xxxed everyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From anh2006 <@t> med.cornell.edu Wed Apr 1 09:19:38 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Wed Apr 1 09:22:19 2009 Subject: [Histonet] PBS poll Message-ID: <1789155362-1238595733-cardhu_decombobulator_blackberry.rim.net-1935756975-@bxe1094.bisx.prod.on.blackberry> For those of you doing immunos, do you use PBS with or without Calcium and Magnesium and why did you make that choice? Thanks! From MElliott <@t> mrl.ubc.ca Wed Apr 1 09:35:59 2009 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Wed Apr 1 09:36:52 2009 Subject: [Histonet] Region IX Education Day Toronto June 2009 Message-ID: <49D3195F.11C6.00D6.0@mrl.ubc.ca> Hi everyone. I forgot to mention in my earlier post about our upcoming Education Day that all the prices quoted are in Canadian dollars, so right now you get a great deal as the Canadian dollar has dropped in value against the US dollar. Mark Elliott Region IX Education Committee Chair ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From SLB <@t> stowers.org Wed Apr 1 09:52:13 2009 From: SLB <@t> stowers.org (Beckham, Sharon) Date: Wed Apr 1 09:52:53 2009 Subject: [Histonet] G 20 MEETING NEWS In-Reply-To: <49D33DD1.2B7F.00C9.0@geisinger.edu> Message-ID: I disagree. This is something I didn't know about and evidently it will effect our profession. No one enjoys getting political more than I do, but I don't consider this as being political talk. I do consider it being newsworthy and something I want to become informed about. Thanks for the heads up as I will want to follow up on this one. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, April 01, 2009 9:11 AM To: histonet@lists.utsouthwestern.edu; Bernie Taupin Subject: Re: [Histonet] G 20 MEETING NEWS Yes please. There are plenty of blogs out there for this kind of thing. >>> Bernie Taupin 4/1/2009 9:53 AM >>> Please refrain from discussing politics on this list. Edwards, R.E. wrote: > Apparently Obama and Brown, with the reluctant compliance of > Germany and > China are aiming to rationalise the pay and conditions of laboratory > workers worldwide, and hopefully if they succeed the histotechs in the > U.S.A. will one be the major beneficiaries!, so fingers xxxed everyone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Wed Apr 1 09:58:47 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 1 09:58:51 2009 Subject: [Histonet] G 20 MEETING NEWS Message-ID: <612135.82414.qm@web43507.mail.sp1.yahoo.com> Sharon, You might like to know, then, that John McCain "thinks histology matters" http://lungcancer.einnews.com/article.php?nid=91 Beckham, Sharon wrote: I disagree. This is something I didn't know about and evidently it will effect our profession. No one enjoys getting political more than I do, but I don't consider this as being political talk. I do consider it being newsworthy and something I want to become informed about. Thanks for the heads up as I will want to follow up on this one. From tifei <@t> foxmail.com Wed Apr 1 10:04:45 2009 From: tifei <@t> foxmail.com (TF) Date: Wed Apr 1 10:05:09 2009 Subject: [Histonet] bovine vs donkey anti-goat IgG References: Message-ID: <200904012304404834184@foxmail.com> Tm8gYmlnIGRpZmZlcmVuY2VzIGluIG15IGhhbmQuDQpJIGV2ZW4gbWlzLXVzZWQgZG9ua2V5IHNl cnVtIGFzIGJsb2NraW5nIHNvbHV0aW9uIHJhdGhlciB0aGFuIGdvYXQgc2VydW0gZm9yIGdvYXQt YW50aS1tb3VzZSBJZ0cgcmVhY3Rpb24uIEJ1dCBubyBiYWNrZ3JvdW5kIGNvbWVzIHVwLg0KU2hv dWxkIGFsc28gY29uc2lkZXIgdGhlIHNwZWNpZmljaXR5IG9mIHlvdXIgYW50aWJvZGllcy4gSWYg dGhleSBhcmUgc3BlY2lmaWMgZW5vdWdoLCBubyBibG9ja2luZyBpcyBpbiBuZWVkIGFjdHVhbGx5 Lg0KDQoNCjIwMDktMDQtMDEgDQoNCg0KDQpURiANCg0KDQoNCreivP7Iy6O6IEFuZHJlYSBIb29w ZXIgDQq3osvNyrG85KO6IDIwMDktMDMtMzAgIDIzOjI4OjAzIA0KytW8/sjLo7ogSGlzdG9uZXQg DQqzrcvNo7ogDQrW98zio7ogW0hpc3RvbmV0XSBib3ZpbmUgdnMgZG9ua2V5IGFudGktZ29hdCBJ Z0cgDQogDQpIYXMgYW55b25lIGNvbXBhcmVkIHRoZSBib3ZpbmUgYW50aS1nb2F0IElnRyB0byB0 aGUgZG9ua2V5IGFudGktZ29hdCANCklnRyBzZWNvbmRhcmllcyBmcm9tIEphY2tzb24gSVIgYW5k IHNlZW4gd2hldGhlciB0aGVyZSB3YXMgYSANCmNvbnNpZGVyYWJsZSBiZW5lZml0IHRvIHVzaW5n IHRoZSBib3ZpbmUgc2VyaWVzPyBNeSBCU0EgaXMgZ2FtbWEgDQpnbG9idWxpbiBmcmVlIHNvIGl0 IHNob3VsZG4ndCBtYWtlIGEgaHVnZSBkaWZmZXJlbmNlIGZvciBtZSwgYnV0IEkgYW0gDQpqdXN0 IHdvbmRlcmluZyAuLi4uLi4uLi4NCkFORFJFQQ0KLS0gDQpfX19fX19fX19fX19fX19fX19fX19f X19fX19fX19fX19fX19fX19fX19fX19fXw0KSGlzdG9uZXQgbWFpbGluZyBsaXN0DQpIaXN0b25l dEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUNCmh0dHA6Ly9saXN0cy51dHNvdXRod2VzdGVybi5l ZHUvbWFpbG1hbi9saXN0aW5mby9oaXN0b25ldA0K From b-frederick <@t> northwestern.edu Wed Apr 1 10:08:30 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Apr 1 10:08:44 2009 Subject: [Histonet] onalighternote In-Reply-To: <241532.54644.qm@web43501.mail.sp1.yahoo.com> Message-ID: <8426B8F258D34AAE84A0034554185572@lurie.northwestern.edu> Bernice, We're not making fun- Bernie Taupin is an excellent and top notch lyricist! Look what he did for Sir Reg! I'd be glad to share my name (and vice versa)with him. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 8:14 AM To: Va Paula Sicurello; Jeanine (CDC/CCID/NCZVED)Bartlett; R.E.Edwards; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] onalighternote I don't know what makes some of you people persist in making fun of a complete stranger's name, but I'll have you know I was born before the person you are talking about. ________________________________ From: Va Paula Sicurello To: Jeanine (CDC/CCID/NCZVED)Bartlett ; R.E.Edwards ; histonet@lists.utsouthwestern.edu; Bernie Taupin Sent: Tuesday, March 31, 2009 7:01:14 PM Subject: Re: [Histonet] onalighternote Hey, we can't help it if your parents were big fans of his. He is a great song writer. ;-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Tue, 3/31/09, Bernie Taupin wrote: > From: Bernie Taupin > Subject: Re: [Histonet] onalighternote > To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , "Edwards, R.E." , histonet@lists.utsouthwestern.edu > Date: Tuesday, March 31, 2009, 4:52 PM > Yeah, hilarious.. I swear, I haven't > heart THAT one before > > > > > ________________________________ > From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" > To: "Edwards, R.E." ; > histonet@lists.utsouthwestern.edu > Sent: Tuesday, March 31, 2009 10:18:36 AM > Subject: RE: [Histonet] onalighternote > > I wanted to ask how Elton was but thought that was a bit > too easy....... > > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Edwards, > R.E. > Sent: Tuesday, March 31, 2009 10:16 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] onalighternote > > > I have just had eeees from Bernie > Taupin and Paul Schofield, is > this a record??. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Robyn > Vazquez > Sent: 31 March 2009 15:08 > To: Bernie Taupin; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Ford Royer > > I think Ford gets the message...GET ON WITH IT! > Antibody talk anyone? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Bernie > Taupin > Sent: Tuesday, March 31, 2009 6:50 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Ford Royer > > > I can personally attest that Ford must have been > having a VERY bad day > indeed. > > Although I can empathize that this might be the case, I do > find it > curious that we've heard nothing from Mr. Royer since his > little > outburst. > > I, for one, wouldn't mind an apology to the list for your > antisocial > behaviour on it, Mr. Royer. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arsenn <@t> hsh.org Wed Apr 1 10:23:58 2009 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Wed Apr 1 10:24:03 2009 Subject: [Histonet] "FREEZY" spray Message-ID: I was taught that freeze spray shouldn't be used - ever - on any tissue at all. We were told that it damages the tissue. Am I wrong?? We don't even use it when doing frozens - we have a different system now that extracts all the warmth and freezes the tissue completely so that we don't need the spray at all. Thanks for everyone's input on this! It will answer a lot of questions in our lab! Amy HT Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From september.amspacher <@t> bassett.org Wed Apr 1 10:29:28 2009 From: september.amspacher <@t> bassett.org (Amspacher, September) Date: Wed Apr 1 10:29:33 2009 Subject: [Histonet] "FREEZY" spray In-Reply-To: Message-ID: <9485D6C8CD957E488B81B56B0EBB28A40180491E@ex3.bassett.org> I was always taught that you never use it when doing frozens because spraying like that into the chamber will aerosolize any particles in the chamber i.e.- TB, as for when cutting only when you have too, it can crack the blocks and stuff like that. September HT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Wednesday, April 01, 2009 11:24 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] "FREEZY" spray I was taught that freeze spray shouldn't be used - ever - on any tissue at all. We were told that it damages the tissue. Am I wrong?? We don't even use it when doing frozens - we have a different system now that extracts all the warmth and freezes the tissue completely so that we don't need the spray at all. Thanks for everyone's input on this! It will answer a lot of questions in our lab! Amy HT Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Apr 1 11:08:30 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Apr 1 11:10:10 2009 Subject: [Histonet] "FREEZY" spray In-Reply-To: Message-ID: Federal registry 1910.1030 ?All procedures involving blood or other potential infectious materials shall be performed in such a manner as to minimize splashing, spraying, spattering, or generation of droplets of these substances.? NCCLS Document M29-T (now CLSI) ?Frozen sections done on unfixed tissue pose a high risk because accidents are common. Freezing of tissue does not inactivate infectious agents. Freezing propellants under pressure should not be used for frozen sections as they may cause spattering of droplets of infectious material.? Based on the above statements, freezing spray should NOT be used in a cryostat with unfixed tissue. Jennifer MacDonald "Senn, Amy R" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/01/2009 08:25 AM To cc Subject [Histonet] "FREEZY" spray I was taught that freeze spray shouldn't be used - ever - on any tissue at all. We were told that it damages the tissue. Am I wrong?? We don't even use it when doing frozens - we have a different system now that extracts all the warmth and freezes the tissue completely so that we don't need the spray at all. Thanks for everyone's input on this! It will answer a lot of questions in our lab! Amy HT Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Wed Apr 1 11:09:57 2009 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Wed Apr 1 11:11:03 2009 Subject: [Histonet] PBS poll In-Reply-To: <1789155362-1238595733-cardhu_decombobulator_blackberry.rim.net-1935756975-@bxe1094.bisx.prod.on.blackberry> References: <1789155362-1238595733-cardhu_decombobulator_blackberry.rim.net-1935756975-@bxe1094.bisx.prod.on.blackberry> Message-ID: PBS with calcium and magnesium is Dulbecco's PBS and was developed specifically to do work with live cells to mimic body fluids. It is not necessary for fixed cells or tissue. Tim Morken Technical Support Manager Immunohistochemistry Anatomical Pathology Thermo Fisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anh2006@med.cornell.edu Sent: Wednesday, April 01, 2009 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PBS poll For those of you doing immunos, do you use PBS with or without Calcium and Magnesium and why did you make that choice? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From collette2 <@t> mail.llnl.gov Wed Apr 1 11:44:03 2009 From: collette2 <@t> mail.llnl.gov (Nicole Collette) Date: Wed Apr 1 11:44:18 2009 Subject: [Histonet] IHC, decalcification preferences Message-ID: Hello, all, I am going to be doing some IHC on adult mouse bone, and since I'm new to all this antigen preservation stuff, was wondering the best way to decalcify? Normally, I use high percentage EDTA (17%) and determine end point by weight loss/weight gain. (up until now have been using this protocol for LacZ stains, works like a charm! ) We are comparing several lines of mice with bone mineral density differences, and endpoint is very phenotype and age-dependent. It takes a long time, but the results are worth the wait. I also have Cal-Rite, which is a formaldehyde/formic acid decalcifier, and obviously works much faster. I am leaning toward the EDTA, since it seems to work well for the LacZ, which is sensitive to lots of other processes, but if there's a reason not to, please let me know. Thanks for all your help and support, I have so far been given great advice from this listserv. Sincerely, Nicole Collette LLNL/UCB From portera <@t> msu.edu Wed Apr 1 11:56:43 2009 From: portera <@t> msu.edu (Amy Porter) Date: Wed Apr 1 11:56:43 2009 Subject: [Histonet] IHC, decalcification preferences References: Message-ID: <0053968BE0214AAB8A4E82848CCF564B@histolab> All the mouse femurs and tibias done in our laboratory are decaled in 14% EDTA and we have a high success rate with our immunohistochemistry and Tunel staining. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Nicole Collette" To: Sent: Wednesday, April 01, 2009 12:44 PM Subject: [Histonet] IHC, decalcification preferences > Hello, all, > > I am going to be doing some IHC on adult mouse bone, and since I'm new to > all this antigen preservation stuff, was wondering the best way to > decalcify? Normally, I use high percentage EDTA (17%) and determine end > point by weight loss/weight gain. (up until now have been using this > protocol for LacZ stains, works like a charm! ) We are comparing several > lines of mice with bone mineral density differences, and endpoint is very > phenotype and age-dependent. It takes a long time, but the results are > worth the wait. I also have Cal-Rite, which is a formaldehyde/formic acid > decalcifier, and obviously works much faster. I am leaning toward the > EDTA, since it seems to work well for the LacZ, which is sensitive to lots > of other processes, but if there's a reason not to, please let me know. > Thanks for all your help and support, I have so far been given great > advice from this listserv. > > Sincerely, > Nicole Collette > LLNL/UCB > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From vapatpxs <@t> yahoo.com Wed Apr 1 12:10:30 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Wed Apr 1 12:10:34 2009 Subject: [Histonet] Let's call a truce Message-ID: <786753.18109.qm@web46112.mail.sp1.yahoo.com> Hello Netters, Time to stop the current off topic conversations and get back to histology. I apologize to Bernie for my comment since I contributed to this off topic topic. It's a new month let's start fresh. Happy slicing and dicing and may all your stains work perfectly. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 From bernietaupin <@t> ymail.com Wed Apr 1 12:35:42 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 1 12:35:47 2009 Subject: [Histonet] Let's call a truce Message-ID: <869954.74129.qm@web43505.mail.sp1.yahoo.com> Nothing like yet another off-topic post to remind us not to post off-topic posts. Ahhh, sweet irony ;-) > Hello Netters, > > Time to stop the current off topic conversations and > get back to histology. > > I apologize to Bernie for my comment since I > contributed to this off topic topic. > > It's a new month let's start fresh. > > Happy slicing and dicing and may all your stains work > perfectly. > > Paula :-) > From dwaugh <@t> kent.edu Wed Apr 1 12:39:33 2009 From: dwaugh <@t> kent.edu (David Waugh) Date: Wed Apr 1 12:39:47 2009 Subject: [Histonet] AO Blade holder In-Reply-To: <961817.71022.qm@web43511.mail.sp1.yahoo.com> References: <91692681-9B86-43BD-B875-ECD59D71F54B@kent.edu> <400079.63960.qm@web43507.mail.sp1.yahoo.com> <2857FFF7-9821-43F1-9DC9-95A7984FDC47@kent.edu> <961817.71022.qm@web43511.mail.sp1.yahoo.com> Message-ID: <69B0428D-B84D-4B32-99FF-488A66C546E7@kent.edu> Again I apologize for what was an honest mistake. I tried looking at the list server home and could not find information regarding the time it takes a post to appear and rules regarding text formats etc. The page I found had only information on how to subscribe or unsubscribe. If there is other information I missed it. -David On Apr 1, 2009, at 1:30 PM, Bernie Taupin wrote: > i guess thats why most people would, you know, read the listserv > instructions before making triplicate posts. speaking of, sorry you > feel like my one note about it has "cluttered up peoples email > boxes" more than your three posts. did you fail math or something? > > From: David Waugh > To: Bernie Taupin > Sent: Wednesday, April 1, 2009 1:15:30 PM > Subject: Re: [Histonet] AO Blade holder > > Sorry for the multiple emails. I did not know how to use THIS > listserv. Some listservers block urls, rich text etc. It was not > clear to me if there were improperly contained with my message. I > was unsure if histonet did, and it was not apparent that the email > was going through. My apologies, although your email and my > response have further cluttered up peoples email boxes. -David > On Apr 1, 2009, at 9:16 AM, Bernie Taupin wrote: > > > Am I getting multiple mails or is it simply that David Waugh > doesn't know how to use a listserv? > > > > > > > > > > ________________________________ > > From: David Waugh > > To: histonet@lists.utsouthwestern.edu > > Sent: Tuesday, March 31, 2009 3:08:08 PM > > Subject: [Histonet] AO Blade holder > > > > > > > >> I have a question regarding a AO disposable blade holder (Cat# > 824). This link should show a picture of a similar > onewww.usedmicroscopes.com/images/AO820Microtome.jpg > >> > >> The blade is tightened with a lever on the right that rotates a > d-shaped cam. Two screws are used the set the tension that the cam > places on the blade, once set, the cam will tighten and loosen the > blade. The back of the holder is concave, and the front part > convex, so that when tightened, the blade will bow. I have not been > able to find any documentation on how tight the blade should be. It > sounds like most holders have flat surfaces that clamp the blade, > which I would think would have very different tightness > requirements. Any ideas from someone who uses this type of holder > on how the two screws should be set? Thanks, David > >> > >> Kent State University > >> Department of Geology > >> Kent, Ohio 44242 > >> dwaugh@kent.edu > >> > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Kent State University > Department of Geology > Kent, Ohio 44242 > dwaugh@kent.edu > www.personal.kent.edu/~dwaugh > > > > Kent State University Department of Geology Kent, Ohio 44242 dwaugh@kent.edu www.personal.kent.edu/~dwaugh From elockman <@t> apsemail.com Wed Apr 1 13:22:08 2009 From: elockman <@t> apsemail.com (Emily Lockman) Date: Wed Apr 1 13:22:13 2009 Subject: [Histonet] deplasticizing Spurr slides Message-ID: <037BDA8D37760D49A2A23D1C877EA8C9AA7D39@apsdc01.aps.dom> I still need help with deplasticizing our Spurr slides. Any advice would be helpful. Also, when cutting Spurr sections, what type of slides (charged, coated, ect.) are people using? Thanks, Emily Emily M. Lockman, HT (ASCP) Histotechnologist II, Pathology Services American Preclinical Services, LLC (APS) 8945 Evergreen Boulevard Minneapolis, MN 55433 Phone: 763-717-7990 Fax: 763-717-2042 elockman@apsemail.com From llewllew <@t> shaw.ca Wed Apr 1 13:26:02 2009 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Wed Apr 1 13:26:38 2009 Subject: [Histonet] Off topic posts In-Reply-To: <869954.74129.qm@web43505.mail.sp1.yahoo.com> References: <869954.74129.qm@web43505.mail.sp1.yahoo.com> Message-ID: <21CBEBA8885048E3878038D16082967B@BryanPC> Off topic posts have been allowed on Histonet since the very beginning. I am not aware that they have ever been banned. After all, what are the TGIF posts but completely off topic silliness for weekend stress relief. Being rude to someone is something else, and shoukl be avoided whether on or off topic. That is just common courtesy. Bryan Llewellyn ----- Original Message ----- From: "Bernie Taupin" To: Sent: Wednesday, April 01, 2009 10:35 AM Subject: Re: [Histonet] Let's call a truce > Nothing like yet another off-topic post to remind us not to post off-topic > posts. > > Ahhh, sweet irony ;-) > > >> Hello Netters, >> >> Time to stop the current off topic conversations and > get back to >> histology. >> >> I apologize to Bernie for my comment since I >> contributed to this off topic topic. >> >> It's a new month let's start fresh. >> >> Happy slicing and dicing and may all your stains work > perfectly. >> >> Paula :-) >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Wed Apr 1 13:45:54 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 1 13:46:01 2009 Subject: [Histonet] Off topic posts In-Reply-To: <21CBEBA8885048E3878038D16082967B@BryanPC> References: <869954.74129.qm@web43505.mail.sp1.yahoo.com> <21CBEBA8885048E3878038D16082967B@BryanPC> Message-ID: <421050.17617.qm@web43513.mail.sp1.yahoo.com> Who are you calling rude, Bruce? The people who made fun of my name? ________________________________ From: Bryan Llewellyn To: Histonet Sent: Wednesday, April 1, 2009 2:26:02 PM Subject: [Histonet] Off topic posts Off topic posts have been allowed on Histonet since the very beginning. I am not aware that they have ever been banned. After all, what are the TGIF posts but completely off topic silliness for weekend stress relief. Being rude to someone is something else, and shoukl be avoided whether on or off topic. That is just common courtesy. Bryan Llewellyn ----- Original Message ----- From: "Bernie Taupin" To: Sent: Wednesday, April 01, 2009 10:35 AM Subject: Re: [Histonet] Let's call a truce > Nothing like yet another off-topic post to remind us not to post off-topic > posts. > > Ahhh, sweet irony ;-) > > >> Hello Netters, >> >> Time to stop the current off topic conversations and > get back to >> histology. >> >> I apologize to Bernie for my comment since I >> contributed to this off topic topic. >> >> It's a new month let's start fresh. >> >> Happy slicing and dicing and may all your stains work > perfectly. >> >> Paula :-) >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lhadley <@t> iupui.edu Wed Apr 1 14:01:03 2009 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Wed Apr 1 14:01:28 2009 Subject: [Histonet] Off topic posts In-Reply-To: <421050.17617.qm@web43513.mail.sp1.yahoo.com> References: <869954.74129.qm@web43505.mail.sp1.yahoo.com> <21CBEBA8885048E3878038D16082967B@BryanPC> <421050.17617.qm@web43513.mail.sp1.yahoo.com> Message-ID: <5E6A94F8037F4F49B738F5B6AD169522132D4E0E04@iu-mssg-mbx09.ads.iu.edu> Hey Bernie...Get over your self. Lee Ann Baldridge IUSM Indpls., IN. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 2:46 PM To: Bryan Llewellyn; Histonet Subject: Re: [Histonet] Off topic posts Who are you calling rude, Bruce? The people who made fun of my name? ________________________________ From: Bryan Llewellyn To: Histonet Sent: Wednesday, April 1, 2009 2:26:02 PM Subject: [Histonet] Off topic posts Off topic posts have been allowed on Histonet since the very beginning. I am not aware that they have ever been banned. After all, what are the TGIF posts but completely off topic silliness for weekend stress relief. Being rude to someone is something else, and shoukl be avoided whether on or off topic. That is just common courtesy. Bryan Llewellyn ----- Original Message ----- From: "Bernie Taupin" To: Sent: Wednesday, April 01, 2009 10:35 AM Subject: Re: [Histonet] Let's call a truce > Nothing like yet another off-topic post to remind us not to post off-topic > posts. > > Ahhh, sweet irony ;-) > > >> Hello Netters, >> >> Time to stop the current off topic conversations and > get back to >> histology. >> >> I apologize to Bernie for my comment since I >> contributed to this off topic topic. >> >> It's a new month let's start fresh. >> >> Happy slicing and dicing and may all your stains work > perfectly. >> >> Paula :-) >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Wed Apr 1 14:12:51 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 1 14:12:56 2009 Subject: [Histonet] Off topic posts In-Reply-To: <5E6A94F8037F4F49B738F5B6AD169522132D4E0E04@iu-mssg-mbx09.ads.iu.edu> References: <869954.74129.qm@web43505.mail.sp1.yahoo.com> <21CBEBA8885048E3878038D16082967B@BryanPC> <421050.17617.qm@web43513.mail.sp1.yahoo.com> <5E6A94F8037F4F49B738F5B6AD169522132D4E0E04@iu-mssg-mbx09.ads.iu.edu> Message-ID: <40560.22584.qm@web43510.mail.sp1.yahoo.com> Speaking of rude, here we have a perfect example: ________________________________ From: "Baldridge, Lee Ann" Hey Bernie...Get over your self. Lee Ann Baldridge IUSM Indpls., IN. From JWeems <@t> sjha.org Wed Apr 1 14:13:12 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Apr 1 14:13:24 2009 Subject: [Histonet] Off topic posts In-Reply-To: <421050.17617.qm@web43513.mail.sp1.yahoo.com> References: <869954.74129.qm@web43505.mail.sp1.yahoo.com><21CBEBA8885048E3878038D16082967B@BryanPC> <421050.17617.qm@web43513.mail.sp1.yahoo.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA542E8E0@ITSSSXM01V6.one.ads.che.org> Who is Bruce? I was completely lost in the name bit... This is hilarious, actually... Let's all have a group laugh, a group hug and get on... Ford was having PMS or being Richard Cranium one or the other... We all do that now and again. My 2 cents again... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 2:46 PM To: Bryan Llewellyn; Histonet Subject: Re: [Histonet] Off topic posts Who are you calling rude, Bruce? The people who made fun of my name? ________________________________ From: Bryan Llewellyn To: Histonet Sent: Wednesday, April 1, 2009 2:26:02 PM Subject: [Histonet] Off topic posts Off topic posts have been allowed on Histonet since the very beginning. I am not aware that they have ever been banned. After all, what are the TGIF posts but completely off topic silliness for weekend stress relief. Being rude to someone is something else, and shoukl be avoided whether on or off topic. That is just common courtesy. Bryan Llewellyn ----- Original Message ----- From: "Bernie Taupin" To: Sent: Wednesday, April 01, 2009 10:35 AM Subject: Re: [Histonet] Let's call a truce > Nothing like yet another off-topic post to remind us not to post off-topic > posts. > > Ahhh, sweet irony ;-) > > >> Hello Netters, >> >> Time to stop the current off topic conversations and > get back to >> histology. >> >> I apologize to Bernie for my comment since I >> contributed to this off topic topic. >> >> It's a new month let's start fresh. >> >> Happy slicing and dicing and may all your stains work > perfectly. >> >> Paula :-) >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From talulahgosh <@t> gmail.com Wed Apr 1 14:14:38 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Apr 1 14:14:43 2009 Subject: [Histonet] Off topic posts In-Reply-To: <5E6A94F8037F4F49B738F5B6AD169522132D4E0E04@iu-mssg-mbx09.ads.iu.edu> References: <869954.74129.qm@web43505.mail.sp1.yahoo.com> <21CBEBA8885048E3878038D16082967B@BryanPC> <421050.17617.qm@web43513.mail.sp1.yahoo.com> <5E6A94F8037F4F49B738F5B6AD169522132D4E0E04@iu-mssg-mbx09.ads.iu.edu> Message-ID: Again, Just repeat to yourself "it's just a list, I should relax" (for mystery science theater threee thousaaaaaaand!) Emily prometheus, thief of light, giver of light, bound by the gods, must have been a book. -mark danielewski, house of leaves From janderson <@t> halozyme.com Wed Apr 1 14:23:11 2009 From: janderson <@t> halozyme.com (Jennifer Anderson) Date: Wed Apr 1 14:23:19 2009 Subject: [Histonet] Off topic posts In-Reply-To: <40560.22584.qm@web43510.mail.sp1.yahoo.com> References: <869954.74129.qm@web43505.mail.sp1.yahoo.com><21CBEBA8885048E3878038D16082967B@BryanPC><421050.17617.qm@web43513.mail.sp1.yahoo.com><5E6A94F8037F4F49B738F5B6AD169522132D4E0E04@iu-mssg-mbx09.ads.iu.edu> <40560.22584.qm@web43510.mail.sp1.yahoo.com> Message-ID: Oh my. If I had known my request for manuals would have sparked such diatribe, I would have withheld. Please stop at once - you're unnecessarily filling my Inbox. This is preposterous. The conversation should be over. I thought this list was monitored??? Jennifer Anderson The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 12:13 PM To: Baldridge, Lee Ann; Bryan Llewellyn; Histonet Subject: Re: [Histonet] Off topic posts Speaking of rude, here we have a perfect example: ________________________________ From: "Baldridge, Lee Ann" Hey Bernie...Get over your self. Lee Ann Baldridge IUSM Indpls., IN. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From batesf <@t> ohsu.edu Wed Apr 1 14:29:05 2009 From: batesf <@t> ohsu.edu (Florence Leomiti) Date: Wed Apr 1 14:30:08 2009 Subject: [Histonet] Off topic posts In-Reply-To: References: <869954.74129.qm@web43505.mail.sp1.yahoo.com><21CBEBA8885048E3878038D16082967B@BryanPC><421050.17617.qm@web43513.mail.sp1.yahoo.com><5E6A94F8037F4F49B738F5B6AD169522132D4E0E04@iu-mssg-mbx09.ads.iu.edu><40560.22584.qm@web43510.mail.sp1.yahoo.com> Message-ID: <0D57C92D2BD3C24ABA05093F55D3EC0593EF7A@EX-BE07.ohsu.edu> People get a life enough already!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson Sent: Wednesday, April 01, 2009 12:23 PM To: Histonet Subject: RE: [Histonet] Off topic posts Oh my. If I had known my request for manuals would have sparked such diatribe, I would have withheld. Please stop at once - you're unnecessarily filling my Inbox. This is preposterous. The conversation should be over. I thought this list was monitored??? Jennifer Anderson The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 12:13 PM To: Baldridge, Lee Ann; Bryan Llewellyn; Histonet Subject: Re: [Histonet] Off topic posts Speaking of rude, here we have a perfect example: ________________________________ From: "Baldridge, Lee Ann" Hey Bernie...Get over your self. Lee Ann Baldridge IUSM Indpls., IN. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Wed Apr 1 14:45:41 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Wed Apr 1 14:45:46 2009 Subject: [Histonet] many thank yous for all the excellent advice! Message-ID: <109DA1E5-9420-4268-A1C5-20CA41F9D7D2@email.arizona.edu> A few weeks ago I asked on Histonet for suggestions on processing and sectioning insects - cockroaches and mosquito GI tracts. After many emails and quite a few phone calls and some further research I was able to get very excellent sections of both and impress the crap out of the customers. ;-) Many thanks to Gayle Callis, Bob Skinner, Barry Rittman, Pam Marcum and Brian Hewlett for all your help and to those who emailed about their experiences with Mollifex and Histogel. Couldn't have pulled this off with out all of you. For the record I have made copious notes so anybody faced with insect processing should feel free to contact me for protocols. Now if anybody has a copy of Barbosa's Manual of Basic Techniques in Insect Histology that they would like to part with pleeeeeze let me know. The book is out of print and I have been borrowing it from the Univ of New Mexico library. Eternally grateful! Andi Grantham next...teeth! From bernietaupin <@t> ymail.com Wed Apr 1 14:49:44 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 1 14:49:49 2009 Subject: [Histonet] Off topic posts In-Reply-To: <0D57C92D2BD3C24ABA05093F55D3EC0593EF7A@EX-BE07.ohsu.edu> References: <869954.74129.qm@web43505.mail.sp1.yahoo.com><21CBEBA8885048E3878038D16082967B@BryanPC><421050.17617.qm@web43513.mail.sp1.yahoo.com><5E6A94F8037F4F49B738F5B6AD169522132D4E0E04@iu-mssg-mbx09.ads.iu.edu><40560.22584.qm@web43510.mail.sp1.yahoo.com> <0D57C92D2BD3C24ABA05093F55D3EC0593EF7A@EX-BE07.ohsu.edu> Message-ID: <927577.96347.qm@web43513.mail.sp1.yahoo.com> >People get a life enough already!!!! Yes, thanks for your valuable, helpful insight, Florence. ________________________________ From: Florence Leomiti To: Jennifer Anderson ; Histonet Sent: Wednesday, April 1, 2009 3:29:05 PM Subject: RE: [Histonet] Off topic posts People get a life enough already!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson Sent: Wednesday, April 01, 2009 12:23 PM To: Histonet Subject: RE: [Histonet] Off topic posts Oh my. If I had known my request for manuals would have sparked such diatribe, I would have withheld. Please stop at once - you're unnecessarily filling my Inbox. This is preposterous. The conversation should be over. I thought this list was monitored??? Jennifer Anderson The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 12:13 PM To: Baldridge, Lee Ann; Bryan Llewellyn; Histonet Subject: Re: [Histonet] Off topic posts Speaking of rude, here we have a perfect example: ________________________________ From: "Baldridge, Lee Ann" Hey Bernie...Get over your self. Lee Ann Baldridge IUSM Indpls., IN. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Wed Apr 1 14:56:30 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Wed Apr 1 14:56:36 2009 Subject: [Histonet] looking for a MAC1 antibody (aka CD11b/CD18, or Integrin alpha-M beta-2) Message-ID: <1814F79F9E53BC3F2C8047C2@bchwxp2702.ad.med.buffalo.edu> I am looking for a good antibody for MAC-1 (CD11b/CD18, Integrin alpha-M beta-2) that is targeted to rodent tissue. Any ideas where I can find one? Thanks! Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From AJohnson <@t> aipathology.com Wed Apr 1 15:00:37 2009 From: AJohnson <@t> aipathology.com (Amy Johnson) Date: Wed Apr 1 15:00:44 2009 Subject: [Histonet] Off topic...lets call a truce Message-ID: <704247D5A09D004C9E6B115138D1703A01829A@hpserv001.aipathology.local> Histonetters, I have been watching and reading these posts all day and I find it all very hilarious. What I have read has not been offensive or rude by any means towards any one individual yet there is one out there who thinks he is being attacked and therefore he thinks he needs to retaliate by insulting others way worse that the original comments had been. Can this all be for real or have I just got caught in an April fools day joke. Just wanted to get in on the fun. Amylin Johnson From gentras <@t> vetmed.auburn.edu Wed Apr 1 15:05:18 2009 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Wed Apr 1 15:05:24 2009 Subject: [Histonet] RE: cost analysis Message-ID: <49D3C8FE.5010505@vetmed.auburn.edu> hello, is there a standard cost analysis for research histopathology which can be used in grant applications? Your prompt reply will be much appreciated. Atoska p.s. histonet archives refers to Rene J. Buesa's article in Jan. 07' Advance. Is advance available on line? From b-frederick <@t> northwestern.edu Wed Apr 1 15:10:36 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Apr 1 15:10:55 2009 Subject: [Histonet] Off topic posts In-Reply-To: <421050.17617.qm@web43513.mail.sp1.yahoo.com> Message-ID: How would we survive without those witticisms of JTT f we disallowed off topic posts? Joe????? Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 1:46 PM To: Bryan Llewellyn; Histonet Subject: Re: [Histonet] Off topic posts Who are you calling rude, Bruce? The people who made fun of my name? ________________________________ From: Bryan Llewellyn To: Histonet Sent: Wednesday, April 1, 2009 2:26:02 PM Subject: [Histonet] Off topic posts Off topic posts have been allowed on Histonet since the very beginning. I am not aware that they have ever been banned. After all, what are the TGIF posts but completely off topic silliness for weekend stress relief. Being rude to someone is something else, and shoukl be avoided whether on or off topic. That is just common courtesy. Bryan Llewellyn ----- Original Message ----- From: "Bernie Taupin" To: Sent: Wednesday, April 01, 2009 10:35 AM Subject: Re: [Histonet] Let's call a truce > Nothing like yet another off-topic post to remind us not to post off-topic > posts. > > Ahhh, sweet irony ;-) > > >> Hello Netters, >> >> Time to stop the current off topic conversations and > get back to >> histology. >> >> I apologize to Bernie for my comment since I >> contributed to this off topic topic. >> >> It's a new month let's start fresh. >> >> Happy slicing and dicing and may all your stains work > perfectly. >> >> Paula :-) >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Wed Apr 1 18:08:40 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 1 18:08:47 2009 Subject: [Histonet] Off topic...lets call a truce In-Reply-To: <704247D5A09D004C9E6B115138D1703A01829A@hpserv001.aipathology.local> References: <704247D5A09D004C9E6B115138D1703A01829A@hpserv001.aipathology.local> Message-ID: <260654.88282.qm@web43516.mail.sp1.yahoo.com> > What I have read has not been offensive or rude by anymeans towards any one individual > he thinks he needs to retaliate by insulting others way worse that the original comments had been is it just me, or do these two statements sound quite contradictory? ________________________________ From: Amy Johnson To: histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 4:00:37 PM Subject: [Histonet] Off topic...lets call a truce Histonetters, I have been watching and reading these posts all day and I find it all very hilarious. What I have read has not been offensive or rude by any means towards any one individual yet there is one out there who thinks he is being attacked and therefore he thinks he needs to retaliate by insulting others way worse that the original comments had been. Can this all be for real or have I just got caught in an April fools day joke. Just wanted to get in on the fun. Amylin Johnson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Apr 1 18:18:48 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Apr 1 18:20:39 2009 Subject: [Histonet] Off topic...lets call a truce In-Reply-To: <260654.88282.qm@web43516.mail.sp1.yahoo.com> References: <704247D5A09D004C9E6B115138D1703A01829A@hpserv001.aipathology.local> <260654.88282.qm@web43516.mail.sp1.yahoo.com> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF04E3@LTA3VS011.ees.hhs.gov> It's not just you -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 7:09 PM To: Amy Johnson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Off topic...lets call a truce > What I have read has not been offensive or rude by anymeans towards > any one individual > he thinks he needs to retaliate by insulting others way worse that the > original comments had been is it just me, or do these two statements sound quite contradictory? ________________________________ From: Amy Johnson To: histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 4:00:37 PM Subject: [Histonet] Off topic...lets call a truce Histonetters, I have been watching and reading these posts all day and I find it all very hilarious. What I have read has not been offensive or rude by any means towards any one individual yet there is one out there who thinks he is being attacked and therefore he thinks he needs to retaliate by insulting others way worse that the original comments had been. Can this all be for real or have I just got caught in an April fools day joke. Just wanted to get in on the fun. Amylin Johnson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Apr 1 18:58:10 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Apr 1 18:58:23 2009 Subject: [Histonet] Off topic posts In-Reply-To: References: Message-ID: <9C9C68BCA923445182F65330E5A13398@JoePC> yeah, what she said ----- Original Message ----- From: "Bernice Frederick" To: "'Bernie Taupin'" ; "'Bryan Llewellyn'" ; "'Histonet'" Sent: Wednesday, April 01, 2009 3:10 PM Subject: RE: [Histonet] Off topic posts How would we survive without those witticisms of JTT f we disallowed off topic posts? Joe????? Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 1:46 PM To: Bryan Llewellyn; Histonet Subject: Re: [Histonet] Off topic posts Who are you calling rude, Bruce? The people who made fun of my name? ________________________________ From: Bryan Llewellyn To: Histonet Sent: Wednesday, April 1, 2009 2:26:02 PM Subject: [Histonet] Off topic posts Off topic posts have been allowed on Histonet since the very beginning. I am not aware that they have ever been banned. After all, what are the TGIF posts but completely off topic silliness for weekend stress relief. Being rude to someone is something else, and shoukl be avoided whether on or off topic. That is just common courtesy. Bryan Llewellyn ----- Original Message ----- From: "Bernie Taupin" To: Sent: Wednesday, April 01, 2009 10:35 AM Subject: Re: [Histonet] Let's call a truce > Nothing like yet another off-topic post to remind us not to post off-topic > posts. > > Ahhh, sweet irony ;-) > > >> Hello Netters, >> >> Time to stop the current off topic conversations and > get back to >> histology. >> >> I apologize to Bernie for my comment since I >> contributed to this off topic topic. >> >> It's a new month let's start fresh. >> >> Happy slicing and dicing and may all your stains work > perfectly. >> >> Paula :-) >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Wed Apr 1 19:01:00 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 1 19:01:23 2009 Subject: [Histonet] Off topic posts In-Reply-To: <9C9C68BCA923445182F65330E5A13398@JoePC> References: <9C9C68BCA923445182F65330E5A13398@JoePC> Message-ID: <275322.49660.qm@web43509.mail.sp1.yahoo.com> What were we talking about again? ________________________________ From: Joe Nocito To: Bernice Frederick ; Bernie Taupin ; Bryan Llewellyn ; Histonet Sent: Wednesday, April 1, 2009 7:58:10 PM Subject: Re: [Histonet] Off topic posts yeah, what she said ----- Original Message ----- From: "Bernice Frederick" To: "'Bernie Taupin'" ; "'Bryan Llewellyn'" ; "'Histonet'" Sent: Wednesday, April 01, 2009 3:10 PM Subject: RE: [Histonet] Off topic posts How would we survive without those witticisms of JTT f we disallowed off topic posts? Joe????? Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 1:46 PM To: Bryan Llewellyn; Histonet Subject: Re: [Histonet] Off topic posts Who are you calling rude, Bruce? The people who made fun of my name? ________________________________ From: Bryan Llewellyn To: Histonet Sent: Wednesday, April 1, 2009 2:26:02 PM Subject: [Histonet] Off topic posts Off topic posts have been allowed on Histonet since the very beginning. I am not aware that they have ever been banned. After all, what are the TGIF posts but completely off topic silliness for weekend stress relief. Being rude to someone is something else, and shoukl be avoided whether on or off topic. That is just common courtesy. Bryan Llewellyn ----- Original Message ----- From: "Bernie Taupin" To: Sent: Wednesday, April 01, 2009 10:35 AM Subject: Re: [Histonet] Let's call a truce > Nothing like yet another off-topic post to remind us not to post off-topic > posts. > > Ahhh, sweet irony ;-) > > >> Hello Netters, >> >> Time to stop the current off topic conversations and > get back to >> histology. >> >> I apologize to Bernie for my comment since I >> contributed to this off topic topic. >> >> It's a new month let's start fresh. >> >> Happy slicing and dicing and may all your stains work > perfectly. >> >> Paula :-) >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Apr 1 19:02:40 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Apr 1 19:02:54 2009 Subject: [Histonet] onalighternote In-Reply-To: <241532.54644.qm@web43501.mail.sp1.yahoo.com> References: <408230.81711.qm@web46106.mail.sp1.yahoo.com> <241532.54644.qm@web43501.mail.sp1.yahoo.com> Message-ID: <15E7E3FA974B49AB88E85EFBD1ABA5D7@JoePC> how about having my name: Giuseppe, Pasquale, Enzo Guido Nocito. I think I was named after a pizza. That's why I call myself Joe. (The toes came later, heh,heh,heh) JTT ----- Original Message ----- From: "Bernie Taupin" To: "Va Paula Sicurello" ; "Jeanine (CDC/CCID/NCZVED)Bartlett" ; "R.E.Edwards" ; Sent: Wednesday, April 01, 2009 8:13 AM Subject: Re: [Histonet] onalighternote >I don't know what makes some of you people persist in making fun of a >complete stranger's name, but I'll have you know I was born before the >person you are talking about. > > > > > ________________________________ > From: Va Paula Sicurello > To: Jeanine (CDC/CCID/NCZVED)Bartlett ; R.E.Edwards > ; histonet@lists.utsouthwestern.edu; Bernie Taupin > > Sent: Tuesday, March 31, 2009 7:01:14 PM > Subject: Re: [Histonet] onalighternote > > > Hey, we can't help it if your parents were big fans of his. He is a great > song writer. > > ;-) > > > Paula Sicurello > VA Medical Center San Diego > Veterans Medical Research Foundation (VMRF) > Core Research Imaging Center > 3350 La Jolla Village Dr., MC151 > San Diego, CA 92161 > 858-552-8585 x2397 > > > --- On Tue, 3/31/09, Bernie Taupin wrote: > >> From: Bernie Taupin >> Subject: Re: [Histonet] onalighternote >> To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , "Edwards, R.E." >> , histonet@lists.utsouthwestern.edu >> Date: Tuesday, March 31, 2009, 4:52 PM >> Yeah, hilarious.. I swear, I haven't >> heart THAT one before >> >> >> >> >> ________________________________ >> From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" >> To: "Edwards, R.E." ; >> histonet@lists.utsouthwestern.edu >> Sent: Tuesday, March 31, 2009 10:18:36 AM >> Subject: RE: [Histonet] onalighternote >> >> I wanted to ask how Elton was but thought that was a bit >> too easy....... >> >> >> >> Jeanine Bartlett >> Infectious Diseases Pathology Branch >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Edwards, >> R.E. >> Sent: Tuesday, March 31, 2009 10:16 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] onalighternote >> >> >> I have just had eeees from Bernie >> Taupin and Paul Schofield, is >> this a record??. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Robyn >> Vazquez >> Sent: 31 March 2009 15:08 >> To: Bernie Taupin; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Ford Royer >> >> I think Ford gets the message...GET ON WITH IT! >> Antibody talk anyone? >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] >> On Behalf Of Bernie >> Taupin >> Sent: Tuesday, March 31, 2009 6:50 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Ford Royer >> >> > I can personally attest that Ford must have been >> having a VERY bad day >> indeed. >> >> Although I can empathize that this might be the case, I do >> find it >> curious that we've heard nothing from Mr. Royer since his >> little >> outburst. >> >> I, for one, wouldn't mind an apology to the list for your >> antisocial >> behaviour on it, Mr. Royer. >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Wed Apr 1 19:38:51 2009 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Wed Apr 1 19:39:40 2009 Subject: [Histonet] Off topic posts In-Reply-To: <421050.17617.qm@web43513.mail.sp1.yahoo.com> References: <869954.74129.qm@web43505.mail.sp1.yahoo.com> <21CBEBA8885048E3878038D16082967B@BryanPC> <421050.17617.qm@web43513.mail.sp1.yahoo.com> Message-ID: My name is Bryan (rhymes with (Ian) not Bruce. I was just making a general comment. Bryan ----- Original Message ----- From: Bernie Taupin To: Bryan Llewellyn ; Histonet Sent: Wednesday, April 01, 2009 11:45 AM Subject: Re: [Histonet] Off topic posts Who are you calling rude, Bruce? The people who made fun of my name? ------------------------------------------------------------------------------ From: Bryan Llewellyn To: Histonet Sent: Wednesday, April 1, 2009 2:26:02 PM Subject: [Histonet] Off topic posts Off topic posts have been allowed on Histonet since the very beginning. I am not aware that they have ever been banned. After all, what are the TGIF posts but completely off topic silliness for weekend stress relief. Being rude to someone is something else, and shoukl be avoided whether on or off topic. That is just common courtesy. Bryan Llewellyn ----- Original Message ----- From: "Bernie Taupin" To: Sent: Wednesday, April 01, 2009 10:35 AM Subject: Re: [Histonet] Let's call a truce > Nothing like yet another off-topic post to remind us not to post off-topic > posts. > > Ahhh, sweet irony ;-) > > >> Hello Netters, >> >> Time to stop the current off topic conversations and > get back to >> histology. >> >> I apologize to Bernie for my comment since I >> contributed to this off topic topic. >> >> It's a new month let's start fresh. >> >> Happy slicing and dicing and may all your stains work > perfectly. >> >> Paula :-) >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Wed Apr 1 20:57:07 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 1 20:57:11 2009 Subject: [Histonet] Off topic posts In-Reply-To: References: <869954.74129.qm@web43505.mail.sp1.yahoo.com> <21CBEBA8885048E3878038D16082967B@BryanPC> <421050.17617.qm@web43513.mail.sp1.yahoo.com> Message-ID: <863069.10851.qm@web43515.mail.sp1.yahoo.com> You should consider going by "Bruce". It suits you better, anyway, as far as I can tell.The last person I knew named "Llewellyn" was... that guy from "The People's Court". Remember that show? http://en.wikipedia.org/wiki/The_People%27s_Court ________________________________ From: Bryan Llewellyn To: Histonet Sent: Wednesday, April 1, 2009 8:38:51 PM Subject: Re: [Histonet] Off topic posts My name is Bryan (rhymes with (Ian) not Bruce. I was just making a general comment. Bryan ----- Original Message ----- From: Bernie Taupin To: Bryan Llewellyn ; Histonet Sent: Wednesday, April 01, 2009 11:45 AM Subject: Re: [Histonet] Off topic posts Who are you calling rude, Bruce? The people who made fun of my name? ------------------------------------------------------------------------------ From: Bryan Llewellyn To: Histonet Sent: Wednesday, April 1, 2009 2:26:02 PM Subject: [Histonet] Off topic posts Off topic posts have been allowed on Histonet since the very beginning. I am not aware that they have ever been banned. After all, what are the TGIF posts but completely off topic silliness for weekend stress relief. Being rude to someone is something else, and shoukl be avoided whether on or off topic. That is just common courtesy. Bryan Llewellyn ----- Original Message ----- From: "Bernie Taupin" To: Sent: Wednesday, April 01, 2009 10:35 AM Subject: Re: [Histonet] Let's call a truce > Nothing like yet another off-topic post to remind us not to post off-topic > posts. > > Ahhh, sweet irony ;-) > > >> Hello Netters, >> >> Time to stop the current off topic conversations and > get back to >> histology. >> >> I apologize to Bernie for my comment since I >> contributed to this off topic topic. >> >> It's a new month let's start fresh. >> >> Happy slicing and dicing and may all your stains work > perfectly. >> >> Paula :-) >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Wed Apr 1 23:04:28 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 1 23:11:14 2009 Subject: [Histonet] Let's call a truce In-Reply-To: <786753.18109.qm@web46112.mail.sp1.yahoo.com> References: <786753.18109.qm@web46112.mail.sp1.yahoo.com> Message-ID: <445593.70778.qm@web43511.mail.sp1.yahoo.com> At this point, I think about 20 or more different people have been involved in this thread! Would anyone else like to chime in with their two-cents about being on-topic or offer their inane peanut-gallery observations and silly tit-for-tat bickering like Paula here? ________________________________ From: Va Paula Sicurello To: HistoNet Sent: Wednesday, April 1, 2009 1:10:30 PM Subject: [Histonet] Let's call a truce Hello Netters, Time to stop the current off topic conversations and get back to histology. I apologize to Bernie for my comment since I contributed to this off topic topic. It's a new month let's start fresh. Happy slicing and dicing and may all your stains work perfectly. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lactose.intolerant <@t> yahoo.com Thu Apr 2 01:13:09 2009 From: lactose.intolerant <@t> yahoo.com (aa aa) Date: Thu Apr 2 01:13:11 2009 Subject: [Histonet] So... I Need Some Expert Advice: Message-ID: <545850.55712.qm@web55301.mail.re4.yahoo.com> Dear Histotech Experts! I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs.... Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! Please view my detailed presentation here: http://tinyurl.com/2g9mqh Thanks Everybody! From bernietaupin <@t> ymail.com Thu Apr 2 01:41:55 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Thu Apr 2 01:41:59 2009 Subject: [Histonet] IHC, decalcification preferences In-Reply-To: <0053968BE0214AAB8A4E82848CCF564B@histolab> References: <0053968BE0214AAB8A4E82848CCF564B@histolab> Message-ID: <68172.37628.qm@web43509.mail.sp1.yahoo.com> I, personally, think EDTA SUCKS. a) it takes too long b) its not safe - http://en.wikipedia.org/wiki/EDTA#Toxicity_and_environmental_considerations c) its to old-fashioned. Me, I'm a big proponent of Formic Acid decal. Holla off-list if you want me to hit you up with a protocol, yo. ________________________________ From: Amy Porter To: histonet@lists.utsouthwestern.edu; Nicole Collette Sent: Wednesday, April 1, 2009 12:56:43 PM Subject: Re: [Histonet] IHC, decalcification preferences All the mouse femurs and tibias done in our laboratory are decaled in 14% EDTA and we have a high success rate with our immunohistochemistry and Tunel staining. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Nicole Collette" To: Sent: Wednesday, April 01, 2009 12:44 PM Subject: [Histonet] IHC, decalcification preferences > Hello, all, > > I am going to be doing some IHC on adult mouse bone, and since I'm new to all this antigen preservation stuff, was wondering the best way to decalcify? Normally, I use high percentage EDTA (17%) and determine end point by weight loss/weight gain. (up until now have been using this protocol for LacZ stains, works like a charm! ) We are comparing several lines of mice with bone mineral density differences, and endpoint is very phenotype and age-dependent. It takes a long time, but the results are worth the wait. I also have Cal-Rite, which is a formaldehyde/formic acid decalcifier, and obviously works much faster. I am leaning toward the EDTA, since it seems to work well for the LacZ, which is sensitive to lots of other processes, but if there's a reason not to, please let me know. Thanks for all your help and support, I have so far been given great advice from this listserv. > > Sincerely, > Nicole Collette > LLNL/UCB > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonet.nospam <@t> vneubert.com Thu Apr 2 03:04:31 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Thu Apr 2 03:04:37 2009 Subject: [Histonet] So... I Need Some Expert Advice: In-Reply-To: <545850.55712.qm@web55301.mail.re4.yahoo.com> References: <545850.55712.qm@web55301.mail.re4.yahoo.com> Message-ID: <4a60cda902546cfb473452301b4c9339@vneubert.com> I suggest to start a board/forum. Really. On Wed, 1 Apr 2009 23:13:09 -0700 (PDT), aa aa wrote: > Dear Histotech Experts! > > I am working on a very complicated project, and could really use some > advice from both the guru-level histologists and also from the go-getter > know-how of the histotechs.... > > Because it's sort of hard to explain (and involves some comparatively > obscure staining techniques and mounting methods) I've put together a short > slide-show to help cut-to-the-chase, as it were, because frankly, some > things are just too tedious, abstract and confusing to type out in text. > > Anyway, on with the show! I would truly and thankfully appreciate any and > all commentary and advice! > > Please view my detailed presentation here: http://tinyurl.com/2g9mqh > > Thanks Everybody! > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From darkdaym <@t> comcast.net Thu Apr 2 04:10:25 2009 From: darkdaym <@t> comcast.net (Mark Ray) Date: Thu Apr 2 03:10:09 2009 Subject: [Histonet] So... I Need Some Expert Advice: In-Reply-To: <545850.55712.qm@web55301.mail.re4.yahoo.com> References: <545850.55712.qm@web55301.mail.re4.yahoo.com> Message-ID: <49D48101.80803@comcast.net> What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: > Dear Histotech Experts! > > I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs.... > > Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. > > Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! > > Please view my detailed presentation here: http://tinyurl.com/2g9mqh > > Thanks Everybody! > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From ian.montgomery <@t> bio.gla.ac.uk Thu Apr 2 06:35:33 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Apr 2 06:38:49 2009 Subject: FW: [Histonet] So... I Need Some Expert Advice: Message-ID: Mark, Histology, who cares, turned up the volume and danced round the office singing along to this Stock, Aitken and Waterman classic. This soul boy even has the album on his iPhone. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Ray Sent: 02 April 2009 10:10 To: lactose.intolerant@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] So... I Need Some Expert Advice: What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: > Dear Histotech Experts! > > I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs.... > > Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. > > Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! > > Please view my detailed presentation here: http://tinyurl.com/2g9mqh > > Thanks Everybody! > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdhisto <@t> yahoo.com Thu Apr 2 06:54:21 2009 From: jdhisto <@t> yahoo.com (acidtone) Date: Thu Apr 2 06:54:26 2009 Subject: [Histonet] Expert advice... Message-ID: I also was directed to some funky video that did not pertan to posted concern. Thats kinda funny. JD From JWeems <@t> sjha.org Thu Apr 2 07:00:09 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Apr 2 07:00:12 2009 Subject: [Histonet] So... I Need Some Expert Advice: In-Reply-To: <49D48101.80803@comcast.net> References: <545850.55712.qm@web55301.mail.re4.yahoo.com> <49D48101.80803@comcast.net> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA542E938@ITSSSXM01V6.one.ads.che.org> I think it was April fool... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Ray Sent: Thursday, April 02, 2009 5:10 AM To: lactose.intolerant@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] So... I Need Some Expert Advice: What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? aa aa wrote: > Dear Histotech Experts! > > I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs.... > > Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. > > Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! > > Please view my detailed presentation here: http://tinyurl.com/2g9mqh > > Thanks Everybody! > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From GDawson <@t> dynacaremilwaukee.com Thu Apr 2 07:52:28 2009 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Apr 2 07:52:33 2009 Subject: [Histonet] Off topic posts In-Reply-To: <40560.22584.qm@web43510.mail.sp1.yahoo.com> Message-ID: KIDS, KIDS!!! Enough is enough. Take it off the list and respond to one another privately. It's fast becoming like observing a daycare here. Glen Dawson IHC Manager Milwaukee, WI From bernietaupin <@t> ymail.com Thu Apr 2 08:52:32 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Thu Apr 2 08:52:36 2009 Subject: [Histonet] Off topic posts In-Reply-To: References: Message-ID: <838134.24361.qm@web43503.mail.sp1.yahoo.com> > Take it off the list and respond to one another privately. Says he.... on the list and not privately ________________________________ From: "Dawson, Glen" To: Histonet Sent: Thursday, April 2, 2009 8:52:28 AM Subject: RE: [Histonet] Off topic posts KIDS, KIDS!!! Enough is enough. Take it off the list and respond to one another privately. It's fast becoming like observing a daycare here. Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Thu Apr 2 09:10:12 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Apr 2 09:10:25 2009 Subject: [Histonet] The end of Histonet In-Reply-To: <445593.70778.qm@web43511.mail.sp1.yahoo.com> References: <786753.18109.qm@web46112.mail.sp1.yahoo.com> <445593.70778.qm@web43511.mail.sp1.yahoo.com> Message-ID: <49D4C744.3020700@umdnj.edu> It is getting difficult to remember when an item that actually dealt with a histotechnology issue was posted. This is how worthwhile discussion groups fail, endless prattle followed by endless prattle about the endless prattle. Geoff -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mcauliff <@t> umdnj.edu Thu Apr 2 09:12:30 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Apr 2 09:12:44 2009 Subject: [Histonet] So... I Need Some Expert Advice: In-Reply-To: <49D48101.80803@comcast.net> References: <545850.55712.qm@web55301.mail.re4.yahoo.com> <49D48101.80803@comcast.net> Message-ID: <49D4C7CE.90803@umdnj.edu> Looked like a scam to me so I hit Delete immediately. No real name, no real address = scam Geoff Mark Ray wrote: > What I got was a Rick Roll music video on Youtube. If aa aa is a > spammer, this was a slick way to attract our attention. Anybody else? > > aa aa wrote: >> Dear Histotech Experts! >> >> I am working on a very complicated project, and could really use some >> advice from both the guru-level histologists and also from the >> go-getter know-how of the histotechs.... >> >> Because it's sort of hard to explain (and involves some comparatively >> obscure staining techniques and mounting methods) I've put together a >> short slide-show to help cut-to-the-chase, as it were, because >> frankly, some things are just too tedious, abstract and confusing to >> type out in text. >> Anyway, on with the show! I would truly and thankfully appreciate any >> and all commentary and advice! >> >> Please view my detailed presentation here: http://tinyurl.com/2g9mqh >> >> Thanks Everybody! >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From jqb7 <@t> cdc.gov Thu Apr 2 09:19:33 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Apr 2 09:19:50 2009 Subject: [Histonet] Fc receptor blocker for staph Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF04EC@LTA3VS011.ees.hhs.gov> Does anyone know where you can purchase an Fc receptor blocker for staph? Vendors welcome! Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From bernietaupin <@t> ymail.com Thu Apr 2 09:21:00 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Thu Apr 2 09:21:05 2009 Subject: [Histonet] Re: The end of Histonet In-Reply-To: <49D4C744.3020700@umdnj.edu> References: <786753.18109.qm@web46112.mail.sp1.yahoo.com> <445593.70778.qm@web43511.mail.sp1.yahoo.com> <49D4C744.3020700@umdnj.edu> Message-ID: <958555.2619.qm@web43508.mail.sp1.yahoo.com> >It is getting difficult to remember when an item that actually dealt with a histotechnology issue was posted. > This is how worthwhile discussion groups fail, endless prattle followed by endless prattle about the endless prattle. And you plan to remedy this by posting more prattle about the prattle about the endless prattle? ________________________________ From: Geoff McAuliffe To: Bernie Taupin Cc: HistoNet Sent: Thursday, April 2, 2009 10:10:12 AM Subject: The end of Histonet It is getting difficult to remember when an item that actually dealt with a histotechnology issue was posted. This is how worthwhile discussion groups fail, endless prattle followed by endless prattle about the endless prattle. Geoff -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From rjbuesa <@t> yahoo.com Thu Apr 2 09:21:43 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 2 09:21:48 2009 Subject: [Histonet] The end of Histonet In-Reply-To: <49D4C744.3020700@umdnj.edu> Message-ID: <266494.52109.qm@web65704.mail.ac4.yahoo.com> Specially when one contributor tries to "outsmart" or "outwit" another or have the "final saying". Ren? J. --- On Thu, 4/2/09, Geoff McAuliffe wrote: From: Geoff McAuliffe Subject: [Histonet] The end of Histonet To: "Bernie Taupin" Cc: "HistoNet" Date: Thursday, April 2, 2009, 10:10 AM It is getting difficult to remember when an item that actually dealt with a histotechnology issue was posted. This is how worthwhile discussion groups fail, endless prattle followed by endless prattle about the endless prattle. Geoff -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Thu Apr 2 09:21:45 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Thu Apr 2 09:28:30 2009 Subject: [Histonet] So... I Need Some Expert Advice: In-Reply-To: <49D4C7CE.90803@umdnj.edu> References: <545850.55712.qm@web55301.mail.re4.yahoo.com> <49D48101.80803@comcast.net> <49D4C7CE.90803@umdnj.edu> Message-ID: <971039.6281.qm@web43508.mail.sp1.yahoo.com> > Looked like a scam to me so I hit Delete immediately. > No real name, no real address = scam It was a link to a video, I believe, not a scam. ________________________________ From: Geoff McAuliffe To: Mark Ray Cc: histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2009 10:12:30 AM Subject: Re: [Histonet] So... I Need Some Expert Advice: Looked like a scam to me so I hit Delete immediately. No real name, no real address = scam Geoff Mark Ray wrote: > What I got was a Rick Roll music video on Youtube. If aa aa is a spammer, this was a slick way to attract our attention. Anybody else? > > aa aa wrote: >> Dear Histotech Experts! >> >> I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs.... >> >> Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. >> Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! >> >> Please view my detailed presentation here: http://tinyurl.com/2g9mqh >> >> Thanks Everybody! >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From boma.fubara <@t> duke.edu Thu Apr 2 09:32:42 2009 From: boma.fubara <@t> duke.edu (Boma Fubara) Date: Thu Apr 2 09:32:52 2009 Subject: [Histonet] glutaraldehyde perfusion Message-ID: Can someone explain to me the mechanism by which glutaraldehyde would disrupt the blood brain barrier if the brain had not been flushed prior to fixation? Boma. From algranth <@t> email.arizona.edu Thu Apr 2 09:33:06 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Thu Apr 2 09:33:12 2009 Subject: [Histonet] So... I Need Some Expert Advice: In-Reply-To: <49D48101.80803@comcast.net> References: <545850.55712.qm@web55301.mail.re4.yahoo.com> <49D48101.80803@comcast.net> Message-ID: <37B5D2BD-2FE3-48B4-91AE-D0F817A1A07F@email.arizona.edu> It wasn't funky it was a good video - but it had no relevance to what we are supposed to be doing here on histonet. Now if somebody could post the link to some Adam Lambert tunes... Andi;-) On Apr 2, 2009, at 2:10 AM, Mark Ray wrote: > What I got was a Rick Roll music video on Youtube. If aa aa is a > spammer, this was a slick way to attract our attention. Anybody else? > > aa aa wrote: >> Dear Histotech Experts! >> >> I am working on a very complicated project, and could really use >> some advice from both the guru-level histologists and also from the >> go-getter know-how of the histotechs.... >> >> Because it's sort of hard to explain (and involves some >> comparatively obscure staining techniques and mounting methods) >> I've put together a short slide-show to help cut-to-the-chase, as >> it were, because frankly, some things are just too tedious, >> abstract and confusing to type out in text. >> Anyway, on with the show! I would truly and thankfully appreciate >> any and all commentary and advice! >> >> Please view my detailed presentation here: http://tinyurl.com/2g9mqh >> >> Thanks Everybody! >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From MSafron <@t> wilresearch.com Wed Apr 1 09:31:00 2009 From: MSafron <@t> wilresearch.com (Mike Safron) Date: Thu Apr 2 09:58:58 2009 Subject: =?us-ascii?Q?=5BHistonet=5D_Help_with_McDowell_and_Trump's_Fixative_4F=3A1G_for_EM=0A=09samples?= Message-ID: <8bdabe69.1c9b2d6.43d46314.50e0@wilresearch.com> Trumps Fixative Ten ml of 40% formaldehyde and 2 ml of 50% glutaraldehyde were added to 200-mOsm buffer. This solution could be conveniently prepared for routine use as follows: 1.16 g of NaH[2]PO[4] is added to 88 ml water and dissolved. Add 0.27 g NaOH followed by the formaldehyde and the glutaraldehyde. Ref. McDowell, E and B. Trump. 1976. Histological fixatives for diagnostic light and electron microscopy. Arch. Pathol. Lab. Med. 100:405-414. Michael A Safron A.S., HT (ASCP)CM WIL Research Laboratories LLC Manager, Histology 419-289-8700 ext 3040 From pathology <@t> hrrmc.net Wed Apr 1 15:55:40 2009 From: pathology <@t> hrrmc.net (Pathology) Date: Thu Apr 2 09:58:59 2009 Subject: [Histonet] Tissue Processor Message-ID: <4EC25783519EC644B2C41CBEC194C372DBAAD15DB9@exchange.hrrmc.net> FREE TBS Tissue Processor (you pay shipping). Kind of a lemon. Liesl Frasier 719-530-2200 x2195 From winston <@t> saclink.csus.edu Thu Apr 2 07:37:34 2009 From: winston <@t> saclink.csus.edu (Lancaster, Winston C) Date: Thu Apr 2 09:59:00 2009 Subject: [Histonet] skin & JB-4 Message-ID: <813DAC550C738E4BBFF73EBFD204DC2C11D8A111C2@VSL4.saclink.csus.edu> Greetings, I have a project sectioning whole bat genital organs. The study, in part, involves measurement and so to avoid shrinkage I am using JB-4 and cutting with a glass knife. The tissue comes out very well except for one problem. The epidermis pulls away from the medium. I have tried leaving the tissue to infiltrate in the catalyzed resin for days and trimming all the little hairs and bristles), to no avail (have not tried bikini waxing as I don't want to destroy the follicles. Later I embedded a patch of skin from the torso and had the same results. Has anyone seen this before? Were you able to solve it? Is there another medium that anyone could suggest that will minimize shrinkage while also adhering to the epidermis? Thanks, Winston Winston C. Lancaster Dept. of Biological Sciences California State University, Sacramento Sacramento, California 95819 6077 wlancaster@csus.edu ________________________________________ From mcauliff <@t> umdnj.edu Thu Apr 2 10:00:14 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Apr 2 10:02:24 2009 Subject: [Histonet] glutaraldehyde perfusion In-Reply-To: References: Message-ID: <49D4D2FE.1050303@umdnj.edu> Hi Boma: Whether or not a saline or buffer flush was used before glut. fixation is of little consequence to the effects of glut as a fixative.The saline/buffer washout is just to remove blood. In my experience most people overdo the washout. I am curious as to what "disrupt the blood-brain barrier" means. The bbb is a very complex entity with both physical and metabolic components.I suspect the person who told you this is not well informed. Geoff Boma Fubara wrote: > Can someone explain to me the mechanism by which glutaraldehyde would > disrupt the blood brain barrier if the brain had not been flushed prior to > fixation? > > Boma. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From algranth <@t> email.arizona.edu Thu Apr 2 10:03:40 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Thu Apr 2 10:03:46 2009 Subject: [Histonet] GemCut Colored Paraffin Message-ID: <27DD738B-76F5-4688-A0E5-F6327F9B012A@email.arizona.edu> Do any of you know if this colored paraffin by Polysciences, Inc. stains the tissue and if so would it auto fluoresce? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello From LINDA.MARGRAF <@t> childrens.com Thu Apr 2 10:18:20 2009 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Thu Apr 2 10:18:28 2009 Subject: [Histonet] Let's call a truce In-Reply-To: <445593.70778.qm@web43511.mail.sp1.yahoo.com> References: <786753.18109.qm@web46112.mail.sp1.yahoo.com> <445593.70778.qm@web43511.mail.sp1.yahoo.com> Message-ID: <49D490EC.F783.00DA.0@childrens.com> Histonet members: As the list moderator, I now feel the need to ask everyone to please get back to the business of histology and related topics. Histonet is run using the University of Texas Southwestern Medical Center's resources and I suspect they would not look favorably on large volumes of off-topic messages. As everyone knows, Histonet is a very useful resource because of the tremendous contributions of all the members (now up to 3200 people) and it would be a shame if a University official happened to review the recent discourse and decide this wasn't a good use of Texas tax dollars. Thanks so much, Linda M Histonet administrator >>> Bernie Taupin 4/1/2009 11:04 PM >>> At this point, I think about 20 or more different people have been involved in this thread! Would anyone else like to chime in with their two-cents about being on-topic or offer their inane peanut-gallery observations and silly tit-for-tat bickering like Paula here? ________________________________ From: Va Paula Sicurello To: HistoNet Sent: Wednesday, April 1, 2009 1:10:30 PM Subject: [Histonet] Let's call a truce Hello Netters, Time to stop the current off topic conversations and get back to histology. I apologize to Bernie for my comment since I contributed to this off topic topic. It's a new month let's start fresh. Happy slicing and dicing and may all your stains work perfectly. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please consider the environment before printing this e-mail

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From contact <@t> excaliburpathology.com Thu Apr 2 10:27:01 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Apr 2 10:27:06 2009 Subject: [Histonet] So... I Need Some Expert Advice: References: <545850.55712.qm@web55301.mail.re4.yahoo.com> <49D48101.80803@comcast.net> <37B5D2BD-2FE3-48B4-91AE-D0F817A1A07F@email.arizona.edu> Message-ID: <336877.80132.qm@web1105.biz.mail.sk1.yahoo.com> Come on people. Yesterday was April Fool's Day and?Histonet got "Rick Rolled". Today is the 2nd, move on. PKP ________________________________ From: Andrea Grantham To: HISTONET Sent: Thursday, April 2, 2009 9:33:06 AM Subject: Re: [Histonet] So... I Need Some Expert Advice: It wasn't funky it was a good video - but it had no relevance to what we are supposed to be doing here on histonet. Now if somebody could post the link to some Adam Lambert tunes... Andi;-) On Apr 2, 2009, at 2:10 AM, Mark Ray wrote: > What I got was a Rick Roll music video on Youtube.? If aa aa is a spammer, this was a slick way to attract our attention.? Anybody else? > > aa aa wrote: >> Dear Histotech Experts! >> >> I am working on a very complicated project, and could really use some advice from both the guru-level histologists and also from the go-getter know-how of the histotechs.... >> >> Because it's sort of hard to explain (and involves some comparatively obscure staining techniques and mounting methods) I've put together a short slide-show to help cut-to-the-chase, as it were, because frankly, some things are just too tedious, abstract and confusing to type out in text. >> Anyway, on with the show! I would truly and thankfully appreciate any and all commentary and advice! >> >> Please view my detailed presentation here: http://tinyurl.com/2g9mqh >> >> Thanks Everybody! >> >> >>? ? ? _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415? ? Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Thu Apr 2 10:44:42 2009 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Apr 2 10:44:48 2009 Subject: [Histonet] So... I Need Some Expert Advice: In-Reply-To: <37B5D2BD-2FE3-48B4-91AE-D0F817A1A07F@email.arizona.edu> References: <545850.55712.qm@web55301.mail.re4.yahoo.com> <49D48101.80803@comcast.net> <37B5D2BD-2FE3-48B4-91AE-D0F817A1A07F@email.arizona.edu> Message-ID: <75A0543E23D3A7458012D9E02EDBEC0002E8077203@UTHCMS1.uthouston.edu> Might I suggest that what we need on Histonet is to deal with some basic questions in histology that have never been answered in detail but have only been touched upon. Histonet has a great deal of expertise and it seems we should be asking these major questions. Topics such as what is optimal time of fixing, best waxes etc. and why should be issues here. My perception is that we often will give personal opinions as to what the best techniques or materials are but not the science behind the rational for choosing these, maybe because we do not know. However I feel that this is an area that we need to expand. Hope that this makes sense. I've had a week of family medical issues, broken plumbing under the house (guess that better than personal plumbing being broken!), two computer crashes and fire ants to name only the major ones. There is therefore, in my opinion, no way to go but up from here so can we hear your views please? Barry From KjLand <@t> usi.edu Thu Apr 2 10:49:16 2009 From: KjLand <@t> usi.edu (Land, Kimberly J) Date: Thu Apr 2 10:50:14 2009 Subject: [Histonet] Mouse mammary glands Message-ID: <5777F3052F84F14296F7B9653A51F24C11AC00@emailnew.usi.edu> Dear histonetters, I am in need of some help. Is it possible to stain a mouse mammary gland in Carmine Alum after it has been fixed in 4% PFA? We originally were fixing them in Carnoy's and then staining, but then it was decided not to stain anymore and the glands were just fixed with the PFA. Now, it looks like the glands will need to be stained, not only the currently procured ones, but also the previous ones as well. If this can be done, does anyone have a procedure or any suggestions? Thank you, Kim Land University of Southern Indiana 8600 University Blvd. Evansville, IN 47712 From Louise_Hartson <@t> URMC.Rochester.edu Thu Apr 2 11:05:39 2009 From: Louise_Hartson <@t> URMC.Rochester.edu (Hartson, Louise) Date: Thu Apr 2 11:05:42 2009 Subject: [Histonet] Embedding cell pellets Message-ID: <1089A756B010074CA09E65CD92401608018A8C8B@MEDMAIL.urmc-sh.rochester.edu> I am looking for a protocol for embedding cell pellets in paraffin. Thanks, Louise Louise Hartson, BA Senior Technical Associate University of Rochester Louise_Hartson@URMC.Rochester.edu From elockman <@t> apsemail.com Thu Apr 2 11:09:13 2009 From: elockman <@t> apsemail.com (Emily Lockman) Date: Thu Apr 2 11:09:21 2009 Subject: [Histonet] GemCut Colored Paraffin In-Reply-To: <27DD738B-76F5-4688-A0E5-F6327F9B012A@email.arizona.edu> References: <27DD738B-76F5-4688-A0E5-F6327F9B012A@email.arizona.edu> Message-ID: <037BDA8D37760D49A2A23D1C877EA8C9B0098A@apsdc01.aps.dom> We use this paraffin and it does not cause any permanent color change to the tissue. When it is removed from the processor after infiltration, the tissue does have some color, but after deparaffinization during staining, there is no color left. Hope this helps. Emily M. Lockman, HT (ASCP) Histotechnologist II, Pathology Services American Preclinical Services, LLC (APS) 8945 Evergreen Boulevard Minneapolis, MN 55433 Phone: 763-717-7990 Fax: 763-717-2042 elockman@apsemail.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Thursday, April 02, 2009 10:04 AM To: HISTONET Subject: [Histonet] GemCut Colored Paraffin Do any of you know if this colored paraffin by Polysciences, Inc. stains the tissue and if so would it auto fluoresce? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonet.nospam <@t> vneubert.com Thu Apr 2 11:12:18 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Thu Apr 2 11:12:22 2009 Subject: [Histonet] Strange circles in IHC slides Message-ID: <49D4E3E2.2010708@vneubert.com> Hello, I really have some real links to real pictures of real relevance of the real histonet list. Really promised. http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg So, has ever anyone experienced sth. like this? My conjugate control (every step except the antibody) was fine, nothing to be seen about DAB and no circles at all. I used Shandon single-use coverplates, sterile buffer, fresh antibody aliquots. Any idea? Thanks, V. Neubert From billodonnell <@t> catholichealth.net Thu Apr 2 11:13:34 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Apr 2 11:13:49 2009 Subject: [Histonet] So... I Need Some Expert Advice: In-Reply-To: <75A0543E23D3A7458012D9E02EDBEC0002E8077203@UTHCMS1.uthouston.edu> References: <545850.55712.qm@web55301.mail.re4.yahoo.com><49D48101.80803@comcast.net><37B5D2BD-2FE3-48B4-91AE-D0F817A1A07F@email.arizona.edu> <75A0543E23D3A7458012D9E02EDBEC0002E8077203@UTHCMS1.uthouston.edu> Message-ID: I'm not in total disagreement with Barry. The FAQ is a good place to start, and it might be all we need, but I wonder it shouldn't be revisited just to be sure. There seems to be a lot of questions lately on some of the basics as well as queries about instrumentation. My question would be this: In light of different internet technologies, would a blog format be an acceptable way of handling or grouping some of these issues? I'm certainly not advocating a change in Histonet, as it is a fine format that is serving the community well. I'm just offering this as a possibile answer to Barry's concern, if others find his concern worth looking into. Just a thought. William (Bill) O'Donnell, HT (ASCP) QIHC Lead Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Thursday, April 02, 2009 10:45 AM To: HISTONET Subject: RE: [Histonet] So... I Need Some Expert Advice: Might I suggest that what we need on Histonet is to deal with some basic questions in histology that have never been answered in detail but have only been touched upon. Histonet has a great deal of expertise and it seems we should be asking these major questions. Topics such as what is optimal time of fixing, best waxes etc. and why should be issues here. My perception is that we often will give personal opinions as to what the best techniques or materials are but not the science behind the rational for choosing these, maybe because we do not know. However I feel that this is an area that we need to expand. Hope that this makes sense. I've had a week of family medical issues, broken plumbing under the house (guess that better than personal plumbing being broken!), two computer crashes and fire ants to name only the major ones. There is therefore, in my opinion, no way to go but up from here so can we hear your views please? Barry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tp2 <@t> medicine.wisc.edu Thu Apr 2 11:17:39 2009 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Thu Apr 2 11:17:56 2009 Subject: [Histonet] Embedding cell pellets Message-ID: <49D49ED3020000DF00016D95@gwmail.medicine.wisc.edu> Fix the cells, spin them down, decant, and resuspend in 1% agarose. The agar plug can be processed and embedded like any other piece of tissue. Tom Pier >>> "Hartson, Louise" 04/02/09 11:08 AM >>> I am looking for a protocol for embedding cell pellets in paraffin. Thanks, Louise Louise Hartson, BA Senior Technical Associate University of Rochester Louise_Hartson@URMC.Rochester.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SDrew <@t> uwhealth.org Thu Apr 2 11:19:24 2009 From: SDrew <@t> uwhealth.org (Drew Sally A) Date: Thu Apr 2 11:19:29 2009 Subject: [Histonet] Hgb A antibody Message-ID: <738A7878143FF74BB77436E255743C1A1F7B68@UWHC-MAIL03.uwhis.hosp.wisc.edu> Anyone have a favorite anti-Hgb A antibody vendor? We're considering bringing this into our inventory of antibodies for formalin-fixed, paraffin-embedded tissue, but of the catalogs(some rather old) we have here I have only found one vendor... Thanks for any opinions on this subject. Sally Ann Drew, MT (ASCP) sdrew@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 From gvdobbin <@t> ihis.org Thu Apr 2 11:23:35 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Apr 2 11:23:58 2009 Subject: [Histonet] Strange circles in IHC slides Message-ID: Hi V. That my friend is the result of a air bubble on the section. If it had been unremoved xylene the hematoxylin would have been absent as well. Cheers. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "V. Neubert" 4/2/2009 1:12:18 PM >>> Hello, I really have some real links to real pictures of real relevance of the real histonet list. Really promised. http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg So, has ever anyone experienced sth. like this? My conjugate control (every step except the antibody) was fine, nothing to be seen about DAB and no circles at all. I used Shandon single-use coverplates, sterile buffer, fresh antibody aliquots. Any idea? Thanks, V. Neubert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From LSebree <@t> uwhealth.org Thu Apr 2 11:30:58 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Apr 2 11:31:03 2009 Subject: [Histonet] Strange circles in IHC slides In-Reply-To: <49D4E3E2.2010708@vneubert.com> Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A5892CDF47@UWHC-MAIL01.uwhis.hosp.wisc.edu> I have seen something (rarely) like this. We always assume that a few bubbles developed that impeded the flow of one or more critical reagents to the tissue underlying the bubble. Somehow, the impediment vanished later in staining to allow for the Hematoxylin counterstain to take. It would only take the omission of one reagent to halt the immunochemical reaction in that area. Just a thought. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of V. Neubert Sent: Thursday, April 02, 2009 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Strange circles in IHC slides Hello, I really have some real links to real pictures of real relevance of the real histonet list. Really promised. http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg So, has ever anyone experienced sth. like this? My conjugate control (every step except the antibody) was fine, nothing to be seen about DAB and no circles at all. I used Shandon single-use coverplates, sterile buffer, fresh antibody aliquots. Any idea? Thanks, V. Neubert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Thu Apr 2 11:33:30 2009 From: jcline <@t> wchsys.org (Joyce Cline) Date: Thu Apr 2 11:33:34 2009 Subject: [Histonet] validation of processors Message-ID: <3ED43B79E67D4BF78B49428FDE3A0836@wchsys.org> Peloris users- how did you validate the protocols on the Peloris against your old processor? The Peloris 6 hour program processes everything so nicely but my VIP is on a 9 ? hr program. How did you validate the IHC? I have processed tissue already but was wondering if we are following what other labs have done. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From histonet.nospam <@t> vneubert.com Thu Apr 2 11:38:33 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Thu Apr 2 11:38:37 2009 Subject: [Histonet] Strange circles in IHC slides In-Reply-To: <49D4E3E2.2010708@vneubert.com> References: <49D4E3E2.2010708@vneubert.com> Message-ID: <49D4EA09.3030607@vneubert.com> Those appear in all 14 slides I stained - I doubt to have made the same mistake of leaving bubbles fourteen times when mounting them. > That my friend is the result of a air bubble on the section. If it had > been unremoved xylene the hematoxylin would have been absent as well. > I have seen something (rarely) like this. We always assume that a few > bubbles developed that impeded the flow of one or more critical reagents > to the tissue underlying the bubble. Somehow, the impediment vanished > later in staining to allow for the Hematoxylin counterstain to take. It > would only take the omission of one reagent to halt the immunochemical > reaction in that area. Just a thought. From collette2 <@t> mail.llnl.gov Thu Apr 2 11:43:04 2009 From: collette2 <@t> mail.llnl.gov (Nicole Collette) Date: Thu Apr 2 11:43:20 2009 Subject: [Histonet] So... I Need Some Expert Advice (forum for information): In-Reply-To: References: <545850.55712.qm@web55301.mail.re4.yahoo.com><49D48101.80803@comcast.net>< 37B5D2BD-2FE3-48B4-91AE-D0F817A1A07F@email.arizona.edu> <75A0543E23D3A7458012D9E02EDBEC0002E8077203@UTHCMS1.uthouston.edu> Message-ID: Hi, All, I'm not generally one to put in my two cents' worth, but today I'm feeling especially chipper. In my world, (idealistic optimist that I am), I would love to see something like a cross between PLoS One, Wikipedia, and Cold Spring Harbor protocols- A mainstream, accepted (or widely used but still not definitive) protocol, or commonly used equipment, with links to discussion for individual steps or reagents. For example, fixing tissue would list a set of reagents, a basic process, and reagents or steps that are often up for debate (Why use NBF? what is paraformaldehyde? what does glutaraldehyde do? How do you get that picric acid yellow out?) would be associated with a link to discussion, so that people who are just looking for the facts can have them readily available, without side comments, and those who want to know the nitty gritty details that cause controversy can make their own judgements, or add their own comments. Now, I'm not at all technologically able to build this resource, but would give anyone who wanted to take it on an "Attaboy!" or "Attagirl! " And if you do, please send me the link ;) There's a lot of good info here, and some really great expertise, but finding it threaded through the archives is not the easiest way to find this stuff, and it is evident by the discussions that come up over and over. Thanks for listening! Keep up the good work! Sincerely, Nicole Collette _ At 10:13 AM -0600 4/2/09, O'Donnell, Bill wrote: >I'm not in total disagreement with Barry. The FAQ is a good place to >start, and it might be all we need, but I wonder it shouldn't be >revisited just to be sure. There seems to be a lot of questions lately >on some of the basics as well as queries about instrumentation. > >My question would be this: > >In light of different internet technologies, would a blog format be an >acceptable way of handling or grouping some of these issues? I'm >certainly not advocating a change in Histonet, as it is a fine format >that is serving the community well. > >I'm just offering this as a possibile answer to Barry's concern, if >others find his concern worth looking into. > >Just a thought. > >William (Bill) O'Donnell, HT (ASCP) QIHC >Lead Histologist >Good Samaritan Hospital >10 East 31st Street >Kearney, NE 68847 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, >Barry R >Sent: Thursday, April 02, 2009 10:45 AM >To: HISTONET >Subject: RE: [Histonet] So... I Need Some Expert Advice: > >Might I suggest that what we need on Histonet is to deal with some basic >questions in histology that have never been answered in detail but have >only been touched upon. >Histonet has a great deal of expertise and it seems we should be asking >these major questions. >Topics such as what is optimal time of fixing, best waxes etc. and why >should be issues here. >My perception is that we often will give personal opinions as to what >the best techniques or materials are but not the science behind the >rational for choosing these, maybe because we do not know. However I >feel that this is an area that we need to expand. >Hope that this makes sense. I've had a week of family medical issues, >broken plumbing under the house (guess that better than personal >plumbing being broken!), two computer crashes and fire ants to name only >the major ones. >There is therefore, in my opinion, no way to go but up from here so can >we hear your views please? >Barry > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http:// lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http:// lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Thu Apr 2 11:30:32 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Apr 2 11:48:41 2009 Subject: [Histonet] Embedding cell pellets Message-ID: <2017207204-1238690916-cardhu_decombobulator_blackberry.rim.net-1871790370-@bxe1094.bisx.prod.on.blackberry> Histogel works well for this purpose too. ------Original Message------ From: Thomas Pier Sender: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu To: Louise_Hartson@URMC.Rochester.edu Sent: Apr 2, 2009 12:17 PM Subject: Re: [Histonet] Embedding cell pellets Fix the cells, spin them down, decant, and resuspend in 1% agarose. The agar plug can be processed and embedded like any other piece of tissue. Tom Pier >>> "Hartson, Louise" 04/02/09 11:08 AM >>> I am looking for a protocol for embedding cell pellets in paraffin. Thanks, Louise Louise Hartson, BA Senior Technical Associate University of Rochester Louise_Hartson@URMC.Rochester.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Thu Apr 2 11:52:28 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Apr 2 11:55:34 2009 Subject: [Histonet] So... I Need Some Expert Advice (forum for information): In-Reply-To: References: <545850.55712.qm@web55301.mail.re4.yahoo.com><49D48101.80803@comcast.net><37B5D2BD-2FE3-48B4-91AE-D0F817A1A07F@email.arizona.edu><75A0543E23D3A7458012D9E02EDBEC0002E8077203@UTHCMS1.uthouston.edu> Message-ID: <702758903-1238691307-cardhu_decombobulator_blackberry.rim.net-1872220632-@bxe1094.bisx.prod.on.blackberry> True true that would be cool. But I don't mind repeated topics being discussed again and again - it isn't always a bad thing, as although histology is a very old and largely well-established science, there is room for improvements and modifications over time.so I appreciate the ongoing discussions over the years, even if a lot of things get repeated. And there ARE a lot of protocols and stable forum websites out there for histo stuff. As fab as Histonet is, it is one of many online resources I consult daily. Andrea -----Original Message----- From: Nicole Collette Date: Thu, 02 Apr 2009 09:43:04 To: HISTONET Subject: RE: [Histonet] So... I Need Some Expert Advice (forum for information): Hi, All, I'm not generally one to put in my two cents' worth, but today I'm feeling especially chipper. In my world, (idealistic optimist that I am), I would love to see something like a cross between PLoS One, Wikipedia, and Cold Spring Harbor protocols- A mainstream, accepted (or widely used but still not definitive) protocol, or commonly used equipment, with links to discussion for individual steps or reagents. For example, fixing tissue would list a set of reagents, a basic process, and reagents or steps that are often up for debate (Why use NBF? what is paraformaldehyde? what does glutaraldehyde do? How do you get that picric acid yellow out?) would be associated with a link to discussion, so that people who are just looking for the facts can have them readily available, without side comments, and those who want to know the nitty gritty details that cause controversy can make their own judgements, or add their own comments. Now, I'm not at all technologically able to build this resource, but would give anyone who wanted to take it on an "Attaboy!" or "Attagirl! " And if you do, please send me the link ;) There's a lot of good info here, and some really great expertise, but finding it threaded through the archives is not the easiest way to find this stuff, and it is evident by the discussions that come up over and over. Thanks for listening! Keep up the good work! Sincerely, Nicole Collette _ At 10:13 AM -0600 4/2/09, O'Donnell, Bill wrote: >I'm not in total disagreement with Barry. The FAQ is a good place to >start, and it might be all we need, but I wonder it shouldn't be >revisited just to be sure. There seems to be a lot of questions lately >on some of the basics as well as queries about instrumentation. > >My question would be this: > >In light of different internet technologies, would a blog format be an >acceptable way of handling or grouping some of these issues? I'm >certainly not advocating a change in Histonet, as it is a fine format >that is serving the community well. > >I'm just offering this as a possibile answer to Barry's concern, if >others find his concern worth looking into. > >Just a thought. > >William (Bill) O'Donnell, HT (ASCP) QIHC >Lead Histologist >Good Samaritan Hospital >10 East 31st Street >Kearney, NE 68847 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, >Barry R >Sent: Thursday, April 02, 2009 10:45 AM >To: HISTONET >Subject: RE: [Histonet] So... I Need Some Expert Advice: > >Might I suggest that what we need on Histonet is to deal with some basic >questions in histology that have never been answered in detail but have >only been touched upon. >Histonet has a great deal of expertise and it seems we should be asking >these major questions. >Topics such as what is optimal time of fixing, best waxes etc. and why >should be issues here. >My perception is that we often will give personal opinions as to what >the best techniques or materials are but not the science behind the >rational for choosing these, maybe because we do not know. However I >feel that this is an area that we need to expand. >Hope that this makes sense. I've had a week of family medical issues, >broken plumbing under the house (guess that better than personal >plumbing being broken!), two computer crashes and fire ants to name only >the major ones. >There is therefore, in my opinion, no way to go but up from here so can >we hear your views please? >Barry > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http:// lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http:// lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barrickstacey <@t> yahoo.com Thu Apr 2 11:55:47 2009 From: barrickstacey <@t> yahoo.com (Stacey Barrick) Date: Thu Apr 2 11:55:50 2009 Subject: [Histonet] low profile blades and cutting angle Message-ID: <683613.92287.qm@web54303.mail.re2.yahoo.com> Hi everyone, ? What brand low profile blades does everyone prefer and where do you order them? ? Also, what blade angle does everyone prefer for cryosectioning? ? Thanks Stacey From histonet.nospam <@t> vneubert.com Thu Apr 2 12:07:38 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Thu Apr 2 12:07:40 2009 Subject: [Histonet] Strange circles in IHC slides Message-ID: <49D4F0DA.8080200@vneubert.com> > > It looks like bubble trapped between the slide and coverplate. Are you using > sufficient surfactant? That can help, but any of the "cap gap" methodologies > lend themselves to this problem. > > Rinse your slides very thoroughly in buffer with plenty of surfactant before > loading them on the instrument. Actually, I don't use any specific surfactant, as long as it is not included in TBS buffer ;) What do you recommend? Maybe just rinsing in buffer would do? I'll give it a try tomorrow, I think. > The bubbles to which I refer to are not coverslipping bubbles, > rather bubbles that were present on the sections during staining that > keep the antibody(ies) and/or the chromogen from making contact with the > tissues. Still, I would think if having that many slides affected, you > probably would have noticed an inordinate amount of bubbles present > between the coverplates and the slides at the time of staining!?! How can they develop? I did not see any bubbles, because I don't change them between the steps. Thanks for your replies :) From vapatpxs <@t> yahoo.com Thu Apr 2 12:08:23 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Thu Apr 2 12:08:30 2009 Subject: [Histonet] onalighternote Message-ID: <680097.25157.qm@web46101.mail.sp1.yahoo.com> Giuseppe, Guido? Enzo, wasn't there a battle of Enzo? Sicurello isn't much better, and Paula as simple as it is can be made into a variety of names and words that start with P. I have also had a variety of spellings of Sicurello-if you can make a rude work out of it-I've seen it. Start with the letter F and go from there. Those Italians, it's all their fault. Paula ;-) aka Paola Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Thu, 4/2/09, Joe Nocito wrote: > From: Joe Nocito > Subject: Re: [Histonet] onalighternote > To: "Bernie Taupin" , "Va Paula Sicurello" , "Jeanine (CDC/CCID/NCZVED)Bartlett" , "R.E.Edwards" , histonet@lists.utsouthwestern.edu > Date: Thursday, April 2, 2009, 12:02 AM > how about having my name: Giuseppe, > Pasquale, Enzo Guido Nocito. I think I > was named after a pizza. That's why I call myself Joe. (The > toes came later, > heh,heh,heh) > > JTT > ----- Original Message ----- > From: "Bernie Taupin" > To: "Va Paula Sicurello" ; > "Jeanine > (CDC/CCID/NCZVED)Bartlett" ; > "R.E.Edwards" > ; > > Sent: Wednesday, April 01, 2009 8:13 AM > Subject: Re: [Histonet] onalighternote > > > >I don't know what makes some of you people persist in > making fun of a > >complete stranger's name, but I'll have you know I was > born before the > >person you are talking about. > > > > > > > > > > ________________________________ > > From: Va Paula Sicurello > > To: Jeanine (CDC/CCID/NCZVED)Bartlett ;? > R.E.Edwards > > ; > histonet@lists.utsouthwestern.edu; > Bernie Taupin > > > > Sent: Tuesday, March 31, 2009 7:01:14 PM > > Subject: Re: [Histonet] onalighternote > > > > > > Hey, we can't help it if your parents were big fans of > his.? He is a great > > song writer. > > > > ;-) > > > > > > Paula Sicurello > > VA Medical Center San Diego > > Veterans Medical Research Foundation (VMRF) > > Core Research Imaging Center > > 3350 La Jolla Village Dr., MC151 > > San Diego, CA 92161 > > 858-552-8585 x2397 > > > > > > --- On Tue, 3/31/09, Bernie Taupin > wrote: > > > >> From: Bernie Taupin > >> Subject: Re: [Histonet] onalighternote > >> To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , > "Edwards, R.E." > >> , > histonet@lists.utsouthwestern.edu > >> Date: Tuesday, March 31, 2009, 4:52 PM > >> Yeah, hilarious.. I swear, I haven't > >> heart THAT one before > >> > >> > >> > >> > >> ________________________________ > >> From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" > >> To: "Edwards, R..E." ; > >> histonet@lists.utsouthwestern.edu > >> Sent: Tuesday, March 31, 2009 10:18:36 AM > >> Subject: RE: [Histonet] onalighternote > >> > >> I wanted to ask how Elton was but thought that was > a bit > >> too easy....... > >> > >> > >> > >> Jeanine Bartlett > >> Infectious Diseases Pathology Branch > >> (404) 639-3590 > >> jeanine.bartlett@cdc.hhs.gov > >> > >> > >> -----Original Message----- > >> From: histonet-bounces@lists.utsouthwestern.edu > >> [mailto:histonet-bounces@lists.utsouthwestern.edu] > >> On Behalf Of Edwards, > >> R.E. > >> Sent: Tuesday, March 31, 2009 10:16 AM > >> To: histonet@lists.utsouthwestern.edu > >> Subject: [Histonet] onalighternote > >> > >> > >> I have? just? had? eeees from? > Bernie > >> Taupin and Paul Schofield, is > >> this? a? record??. > >> > >> -----Original Message----- > >> From: histonet-bounces@lists.utsouthwestern.edu > >> [mailto:histonet-bounces@lists.utsouthwestern.edu] > >> On Behalf Of Robyn > >> Vazquez > >> Sent: 31 March 2009 15:08 > >> To: Bernie Taupin; histonet@lists.utsouthwestern.edu > >> Subject: RE: [Histonet] Ford Royer > >> > >> I think Ford gets the message...GET ON WITH IT! > >> Antibody talk anyone? > >> > >> -----Original Message----- > >> From: histonet-bounces@lists.utsouthwestern.edu > >> [mailto:histonet-bounces@lists.utsouthwestern.edu] > >> On Behalf Of Bernie > >> Taupin > >> Sent: Tuesday, March 31, 2009 6:50 AM > >> To: histonet@lists.utsouthwestern.edu > >> Subject: [Histonet] Ford Royer > >> > >> > I can personally attest that Ford must have > been > >> having a VERY bad day > >> indeed. > >> > >> Although I can empathize that this might be the > case, I do > >> find it > >> curious that we've heard nothing from Mr. Royer > since his > >> little > >> outburst. > >> > >> I, for one, wouldn't mind an apology to the list > for your > >> antisocial > >> behaviour on it, Mr. Royer. > >> > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From vapatpxs <@t> yahoo.com Thu Apr 2 12:09:57 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Thu Apr 2 12:10:02 2009 Subject: [Histonet] Let's call a truce Message-ID: <882834.88475.qm@web46108.mail.sp1.yahoo.com> Boy Bernie, See if I ever buy your albums again! ;-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Thu, 4/2/09, Bernie Taupin wrote: > From: Bernie Taupin > Subject: Re: [Histonet] Let's call a truce > To: "HistoNet" > Date: Thursday, April 2, 2009, 4:04 AM > At this point,? I think about 20 > or more different people have been involved in this thread! > > Would anyone else like to chime in with their two-cents > about being on-topic or offer their inane peanut-gallery > observations and silly tit-for-tat bickering like Paula > here? > > > > ________________________________ > From: Va Paula Sicurello > To: HistoNet > Sent: Wednesday, April 1, 2009 1:10:30 PM > Subject: [Histonet] Let's call a truce > > > Hello Netters, > > Time to stop the current off topic conversations and get > back to histology. > > I apologize to Bernie for my comment since I contributed to > this off topic topic. > > It's a new month let's start fresh. > > Happy slicing and dicing and may all your stains work > perfectly. > > Paula? :-) > > Paula Sicurello > VA Medical Center San Diego > Veterans Medical Research Foundation (VMRF) > Core Research Imaging Center > 3350 La Jolla Village Dr., MC151 > San Diego, CA 92161 > 858-552-8585 x2397 > > > ? ? ? > > > _______________________________________________ > Histonet mailing list > Histonet@lists..utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ? ? ? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern..edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From yvan_lindekens <@t> yahoo.com Thu Apr 2 12:13:31 2009 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Thu Apr 2 12:13:36 2009 Subject: [Histonet] Dummy question on the old Fisher Histomatic tissue processor 166A Message-ID: <32614.76289.qm@web110212.mail.gq1.yahoo.com> Hi all, What exactly is it supposed to do when the switch "Process mode" ("start program-drain-purge") switch is set to "purge"? I aquired this one allmost for free and I will use it - once it's up and running - in the first place for plant anatomy. Yep: it's some kind of a museum around here. Yvan. From leiker <@t> buffalo.edu Thu Apr 2 12:16:45 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Thu Apr 2 12:16:50 2009 Subject: [Histonet] low profile blades and cutting angle In-Reply-To: <683613.92287.qm@web54303.mail.re2.yahoo.com> References: <683613.92287.qm@web54303.mail.re2.yahoo.com> Message-ID: <5632BA518707563D748984EE@bchwxp2702.ad.med.buffalo.edu> AccuEdge by Sakura Finetek are the best (avail. through VWR) Not only have I tried a few other brands, but our former knowledgeable and experienced histotech here at UB recommends AccuEdge. Merced --On Thursday, April 02, 2009 9:55 AM -0700 Stacey Barrick wrote: > Hi everyone, > ? > What brand low profile blades does everyone prefer and where do you order > them? ? > Also, what blade angle does everyone prefer for cryosectioning? > ? > Thanks > > Stacey > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From b-frederick <@t> northwestern.edu Thu Apr 2 13:18:39 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Apr 2 13:18:51 2009 Subject: [Histonet] Embedding cell pellets In-Reply-To: <49D49ED3020000DF00016D95@gwmail.medicine.wisc.edu> Message-ID: <71364FDF3A804F679AB0AAAA524C13FE@lurie.northwestern.edu> Try histogel from Richard Allan- available from Fisher.Easier than making up agarose! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Pier Sent: Thursday, April 02, 2009 11:18 AM To: histonet@lists.utsouthwestern.edu; Louise_Hartson@URMC.Rochester.edu Subject: Re: [Histonet] Embedding cell pellets Fix the cells, spin them down, decant, and resuspend in 1% agarose. The agar plug can be processed and embedded like any other piece of tissue. Tom Pier >>> "Hartson, Louise" 04/02/09 11:08 AM >>> I am looking for a protocol for embedding cell pellets in paraffin. Thanks, Louise Louise Hartson, BA Senior Technical Associate University of Rochester Louise_Hartson@URMC.Rochester.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu Apr 2 13:19:48 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Apr 2 13:20:00 2009 Subject: [Histonet] low profile blades and cutting angle In-Reply-To: <5632BA518707563D748984EE@bchwxp2702.ad.med.buffalo.edu> Message-ID: <204565E9CCFE4F62B1C2A3DE68F608D0@lurie.northwestern.edu> It's all I ever use and I've tried a bunch. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Thursday, April 02, 2009 12:17 PM To: barrickstacey@yahoo.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] low profile blades and cutting angle AccuEdge by Sakura Finetek are the best (avail. through VWR) Not only have I tried a few other brands, but our former knowledgeable and experienced histotech here at UB recommends AccuEdge. Merced --On Thursday, April 02, 2009 9:55 AM -0700 Stacey Barrick wrote: > Hi everyone, > ? > What brand low profile blades does everyone prefer and where do you order > them? ? > Also, what blade angle does everyone prefer for cryosectioning? > ? > Thanks > > Stacey > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Steven.Joy <@t> capitalhealth.ca Thu Apr 2 13:30:47 2009 From: Steven.Joy <@t> capitalhealth.ca (Steven Joy) Date: Thu Apr 2 13:30:54 2009 Subject: [Histonet] GemCut Parafin Message-ID: <1101A09566B25D4BB3889A44FDC1E7040A9AD2@uantexchg03.capitalhealth.ca> Does anyone use GemCut parafin, and what do you think about it, any good, any concerns? Steve From thisisann <@t> aol.com Thu Apr 2 13:33:06 2009 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Thu Apr 2 13:33:23 2009 Subject: [Histonet] Burned Tissue Message-ID: <8CB81D89985EA77-1380-1BCB@webmail-md08.sysops.aol.com> Recently we are experiencing random blocks of tissue (approx. 3 blocks out of 400/2x) that appear to be "burned".? The tissue is prostate and the staining looks dark.? Upon recutting, sometimes it is better, sometimes it is not, which negates my ability to rule out the stainer completely. We process on a 2 hour schedule using the Peloris and all tissue is processed together.? I am not understanding how, out of a 12 part prostate, only one block stains so dark so randomly.? When recut, some blocks are better, some still have the dark staining. Does anyone have any suggestions? Ann From resendes.ana <@t> gmail.com Thu Apr 2 13:39:58 2009 From: resendes.ana <@t> gmail.com (Ana Resendes) Date: Thu Apr 2 13:40:03 2009 Subject: [Histonet] marine animals and insects/ arthrops histotechnology Message-ID: Anyone out there expertize or with a good background or working experience in marine (fish, algae, shell moluscles crustaceans) and insects or arthropds histotechnology ? Thank you. Ana -- Ana Resendes DVM, MSc, PhD Veterinary Pathologist Centro de Investiga??o de Recursos Naturais (CIRN) Sec??o de Anatomia e Taxonomia Zool?gicas, Departamento de Biologia, Universidade dos A?ores. Rua da M?e de Deus, 58 - Apartado 1422 P - 9501-801 Ponta Delgada (A?ores) Portugal Tel. (+351) 296 650 000 ext. 1109 Fax (+351) 296 650 100 http://www.uac.pt/~pherg/ From JMacDonald <@t> mtsac.edu Thu Apr 2 13:52:01 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Apr 2 13:53:42 2009 Subject: [Histonet] Burned Tissue In-Reply-To: <8CB81D89985EA77-1380-1BCB@webmail-md08.sysops.aol.com> Message-ID: We had this problem in the past, but it was attributed to the excessive use of freezing spray. The tissue was basically freezer burnt. Jennifer thisisann@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 04/02/2009 11:35 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Burned Tissue Recently we are experiencing random blocks of tissue (approx. 3 blocks out of 400/2x) that appear to be "burned".? The tissue is prostate and the staining looks dark.? Upon recutting, sometimes it is better, sometimes it is not, which negates my ability to rule out the stainer completely. We process on a 2 hour schedule using the Peloris and all tissue is processed together.? I am not understanding how, out of a 12 part prostate, only one block stains so dark so randomly.? When recut, some blocks are better, some still have the dark staining. Does anyone have any suggestions? Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Apr 2 14:22:09 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Apr 2 14:22:13 2009 Subject: [Histonet] low profile blades and cutting angle In-Reply-To: <204565E9CCFE4F62B1C2A3DE68F608D0@lurie.northwestern.edu> References: <5632BA518707563D748984EE@bchwxp2702.ad.med.buffalo.edu> <204565E9CCFE4F62B1C2A3DE68F608D0@lurie.northwestern.edu> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D830FF@EMAIL.archildrens.org> We use MX premier microtome blades and order from ThermoFisher. I do have the accuedge blades but prefer the MX blades. The accuedge blades just don't seem to be as good as they used to be...my opinion only. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, April 02, 2009 1:20 PM To: 'Merced Leiker'; barrickstacey@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] low profile blades and cutting angle It's all I ever use and I've tried a bunch. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Thursday, April 02, 2009 12:17 PM To: barrickstacey@yahoo.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] low profile blades and cutting angle AccuEdge by Sakura Finetek are the best (avail. through VWR) Not only have I tried a few other brands, but our former knowledgeable and experienced histotech here at UB recommends AccuEdge. Merced --On Thursday, April 02, 2009 9:55 AM -0700 Stacey Barrick wrote: > Hi everyone, > ? > What brand low profile blades does everyone prefer and where do you order > them? ? > Also, what blade angle does everyone prefer for cryosectioning? > ? > Thanks > > Stacey > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From laurie.colbert <@t> huntingtonhospital.com Thu Apr 2 14:38:01 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Apr 2 14:38:07 2009 Subject: [Histonet] HT licensing in 2011 Message-ID: <57BE698966D5C54EAE8612E8941D7683055536D4@EXCHANGE3.huntingtonhospital.com> Does anyone know about a law that is supposed to go into effect in 2011 that will require histotechs to be licensed? I also heard something about a person being eligible to take the HT exam if they get a state license - which can be obtained by simply paying a fee if they have 5 years of work experience? Laurie Colbert From mucram11 <@t> comcast.net Thu Apr 2 14:41:40 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Thu Apr 2 14:41:43 2009 Subject: [Histonet] low profile blades and cutting angle In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D830FF@EMAIL.archildrens.org> Message-ID: <1727270388.1342991238701300662.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I am with you Hazel.? We cut paraffin and today I am cutting GMA plastic with them.? They are great for us. Pam Marcum UPENN Vet School New Bolton Center ----- Original Message ----- From: "Hazel V Horn" To: histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2009 3:22:09 PM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] low profile blades and cutting angle We use MX premier microtome blades and order from ThermoFisher. ? ? I do have the accuedge blades but prefer the MX blades. ? The accuedge blades just don't seem to be as good as they used to be...my opinion only. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way ? ?Slot 820 Little Rock, AR ? 72202 ? phone ? 501.364.4240 fax ? ? ? ?501.364.3155 ? visit us on the web at: ? ?www.archildrens.org ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, April 02, 2009 1:20 PM To: 'Merced Leiker'; barrickstacey@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] low profile blades and cutting angle It's all I ever use and I've tried a bunch. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Thursday, April 02, 2009 12:17 PM To: barrickstacey@yahoo.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] low profile blades and cutting angle AccuEdge by Sakura Finetek are the best (avail. through VWR) Not only have I tried a few other brands, but our former knowledgeable and experienced histotech here at UB recommends AccuEdge. Merced --On Thursday, April 02, 2009 9:55 AM -0700 Stacey Barrick wrote: > Hi everyone, > ? > What brand low profile blades does everyone prefer and where do you order > them? ? > Also, what blade angle does everyone prefer for cryosectioning? > ? > Thanks > > Stacey > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hlukey <@t> msn.com Thu Apr 2 14:47:03 2009 From: hlukey <@t> msn.com (Hugh Luk) Date: Thu Apr 2 14:47:07 2009 Subject: [Histonet] low profile blades and cutting angle In-Reply-To: <204565E9CCFE4F62B1C2A3DE68F608D0@lurie.northwestern.edu> References: <5632BA518707563D748984EE@bchwxp2702.ad.med.buffalo.edu> <204565E9CCFE4F62B1C2A3DE68F608D0@lurie.northwestern.edu> Message-ID: Stacey, Same for me too with Accuedge! But I also like "Shandon MX35 Premier." Pack of 50 blades may be over $100 (depends on your Fisher pricing). I use these for both microtome and cyrostat sections. I've also used these for MMA sections. Premier Plus should be good also, but I haven't tried it. 30-518-35 Thermo Scientific No.:3051835 Blade angle? About 30 degrees. Thermo states 34 degrees in their catalog. I think I've seen Accuedge blades thru several vendor sites. Cardinal, Fisher, VWR, Electron Microscopy Sciences, ect. For example: http://www.emsdiasum.com/microscopy/products/histology/sectioning.aspx Hugh KP-Hawaii > From: b-frederick@northwestern.edu > To: leiker@buffalo.edu; barrickstacey@yahoo.com; histonet@lists.utsouthwestern.edu > Date: Thu, 2 Apr 2009 13:19:48 -0500 > Subject: RE: [Histonet] low profile blades and cutting angle > CC: > > It's all I ever use and I've tried a bunch. > Bernice > > > Bernice Frederick HTL (ASCP) > Northwestern University > Pathology Core Facility > ECOGPCO-RL > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced > Leiker > Sent: Thursday, April 02, 2009 12:17 PM > To: barrickstacey@yahoo.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] low profile blades and cutting angle > > AccuEdge by Sakura Finetek are the best (avail. through VWR) > > Not only have I tried a few other brands, but our former knowledgeable and > experienced histotech here at UB recommends AccuEdge. > > Merced > > --On Thursday, April 02, 2009 9:55 AM -0700 Stacey Barrick > wrote: > > > Hi everyone, > > > > What brand low profile blades does everyone prefer and where do you order > > them? > > Also, what blade angle does everyone prefer for cryosectioning? > > > > Thanks > > > > Stacey > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ Rediscover Hotmail?: Now available on your iPhone or BlackBerry http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Mobile1_042009 From alyssa <@t> alliedsearchpartners.com Thu Apr 2 14:48:53 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Apr 2 14:48:57 2009 Subject: [Histonet] Question: Slides/Day? Message-ID: Hello Histonetters, I understand most of the professionals on this website are histology professionals, however, I thought I would give it a try since I have not found a cytology listserv yet. Does anyone know how many slides per day a cytotechnologist would screen within a private lab setting (On average)? -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 From CBark <@t> memorialcare.org Thu Apr 2 14:50:12 2009 From: CBark <@t> memorialcare.org (Christine Bark) Date: Thu Apr 2 14:50:29 2009 Subject: [Histonet] Davidson's fixative Message-ID: Hello Histonetters! I was wondering if any one knows of any studies done on Davidson's fixative and breast IHC. We have been using Davidson's for our colons for about two years now (thanks to the Histonet) and I would like to expand its use to our very fatty breast cases. Our head pathologist is worried that it will affect the IHC. Thank you all in advance. Christine Bark HT(ASCP) CM Lead Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Bonnie.Whitaker <@t> osumc.edu Thu Apr 2 14:58:48 2009 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Thu Apr 2 14:59:04 2009 Subject: [Histonet] low profile blades and cutting angle In-Reply-To: <1727270388.1342991238701300662.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D830FF@EMAIL.archildrens.org> <1727270388.1342991238701300662.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F60D6629B@msxc06.OSUMC.EDU> I like both, but use the MX because they are cheaper, and they are, if not quite as good as, certainly a much greater value than the AccuEdge blades (I do think that on average I go through the MX a little faster, but they are still cheaper!!). I alsoo like the MB22 from ThermoFisher for cutting small, thin sections, like renal bx. Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 Bonnie.Whitaker@osumc.edu phone 614.293.5048 fax 614.293.7273 pager 614.346.5013 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, April 02, 2009 3:42 PM To: Hazel V Horn Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] low profile blades and cutting angle I am with you Hazel.? We cut paraffin and today I am cutting GMA plastic with them.? They are great for us. Pam Marcum UPENN Vet School New Bolton Center ----- Original Message ----- From: "Hazel V Horn" To: histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2009 3:22:09 PM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] low profile blades and cutting angle We use MX premier microtome blades and order from ThermoFisher. ? ? I do have the accuedge blades but prefer the MX blades. ? The accuedge blades just don't seem to be as good as they used to be...my opinion only. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way ? ?Slot 820 Little Rock, AR ? 72202 ? phone ? 501.364.4240 fax ? ? ? ?501.364.3155 ? visit us on the web at: ? ?www.archildrens.org ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, April 02, 2009 1:20 PM To: 'Merced Leiker'; barrickstacey@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] low profile blades and cutting angle It's all I ever use and I've tried a bunch. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Thursday, April 02, 2009 12:17 PM To: barrickstacey@yahoo.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] low profile blades and cutting angle AccuEdge by Sakura Finetek are the best (avail. through VWR) Not only have I tried a few other brands, but our former knowledgeable and experienced histotech here at UB recommends AccuEdge. Merced --On Thursday, April 02, 2009 9:55 AM -0700 Stacey Barrick wrote: > Hi everyone, > ? > What brand low profile blades does everyone prefer and where do you > order them? > Also, what blade angle does everyone prefer for cryosectioning? > ? > Thanks > > Stacey > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************************** ***************************************************************************** ***************************************************************************** ***************************************************************************** ***************************************************************************** ***************************************************************************** ***************************************************************************** ***************************************************************************** ************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Apr 2 15:14:23 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 2 15:14:28 2009 Subject: [Histonet] So... I Need Some Expert Advice: In-Reply-To: <75A0543E23D3A7458012D9E02EDBEC0002E8077203@UTHCMS1.uthouston.edu> Message-ID: <572893.59886.qm@web65715.mail.ac4.yahoo.com> Barry: Agree. Pick up? ONE theme and ask for everybody to contribute. If satisfactorily covered, pick another and so on. Ren? J. --- On Thu, 4/2/09, Rittman, Barry R wrote: From: Rittman, Barry R Subject: RE: [Histonet] So... I Need Some Expert Advice: To: "HISTONET" Date: Thursday, April 2, 2009, 11:44 AM Might I suggest that what we need on Histonet is to deal with some basic questions in histology that have never been answered in detail but have only been touched upon. Histonet has a great deal of expertise and it seems we should be asking these major questions. Topics such as what is optimal time of fixing, best waxes etc. and why should be issues here. My perception is that we often will give personal opinions as to what the best techniques or materials are but not the science behind the rational for choosing these, maybe because we do not know. However I feel that this is an area that we need to expand. Hope that this makes sense. I've had a week of family medical issues, broken plumbing under the house (guess that better than personal plumbing being broken!), two computer crashes and fire ants to name only the major ones. There is therefore, in my opinion, no way to go but up from here so can we hear your views please? Barry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Thu Apr 2 15:35:02 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Apr 2 15:36:50 2009 Subject: [Histonet] HT licensing in 2011 In-Reply-To: <57BE698966D5C54EAE8612E8941D7683055536D4@EXCHANGE3.huntingtonhospital.com> Message-ID: Laurie, The bill that everyone has been "quoting" is AB 2156. It does not state that histotechnicians will have to be licensed. The ASCP has specific eligibility requirements for HT certification and at this point they are not related to licensure in any state. Other states that have licensure do not just accept payment for the license. They require some form of competency exam. If a person is already ASCP certified than in some states they will be granted licensure. This is an excerpt from the bill. Under existing law, unlicensed laboratory personnel are authorized to perform specified activities, in a licensed clinical laboratory, under the direct and constant supervision of a physician and surgeon or another licensed person if certain criteria are met. Existing law authorizes these unlicensed laboratory personnel to perform specimen labeling, handling, preservation or fixation, processing or preparation, transportation, and storing. This bill would authorize a certified pathologists' assistant, within the specialty of pathology, demonstrating specified competency, to perform specified activities under the supervision and control of a pathologist. The bill would authorize a pathologists' assistant, a histologic technician, and a histotechnologist to prepare human surgical specimens for gross description and dissection under the direct supervision, as defined, of a qualified pathologist, if he or she meets specified requirements. The bill would authorize the department, on and after January 1, 2011, to adopt regulations establishing additional qualification requirements to perform the duties specified in these provisions. "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/02/2009 12:38 PM To "histonet" cc Subject [Histonet] HT licensing in 2011 Does anyone know about a law that is supposed to go into effect in 2011 that will require histotechs to be licensed? I also heard something about a person being eligible to take the HT exam if they get a state license - which can be obtained by simply paying a fee if they have 5 years of work experience? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Thu Apr 2 15:38:21 2009 From: jcline <@t> wchsys.org (Joyce Cline) Date: Thu Apr 2 15:38:29 2009 Subject: [Histonet] Question: Slides/Day? In-Reply-To: Message-ID: <76452FD25660487796C8533613565A8D@wchsys.org> Our cytotechs screen 40 to 60 a day. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alyssa Peterson Sent: Thursday, April 02, 2009 3:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question: Slides/Day? Hello Histonetters, I understand most of the professionals on this website are histology professionals, however, I thought I would give it a try since I have not found a cytology listserv yet. Does anyone know how many slides per day a cytotechnologist would screen within a private lab setting (On average)? -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From rjbuesa <@t> yahoo.com Thu Apr 2 15:48:00 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 2 15:48:05 2009 Subject: [Histonet] Davidson's fixative In-Reply-To: Message-ID: <493833.28124.qm@web65702.mail.ac4.yahoo.com> Christine: Unfortunately our profession has no standards with regards of how we do things, we tend to add "twists" even to recognized procesures, is in our histotechs DNA. Each histolab processes tissues mostly according to its own experience?with previous results. So I think that the best for you, instead of relying on others experiences, you should try to test in parallel using some cases that you will process in Davidson and using whatever you are using now, including the IHC. That first hand experience I think will be much more helpful for you. Ren? J. --- On Thu, 4/2/09, Christine Bark wrote: From: Christine Bark Subject: [Histonet] Davidson's fixative To: histonet@lists.utsouthwestern.edu Date: Thursday, April 2, 2009, 3:50 PM Hello Histonetters! I was wondering if any one knows of any studies done on Davidson's fixative and breast IHC. We have been using Davidson's for our colons for about two years now (thanks to the Histonet) and I would like to expand its use to our very fatty breast cases. Our head pathologist is worried that it will affect the IHC. Thank you all in advance. Christine Bark HT(ASCP) CM Lead Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Apr 2 16:33:37 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Apr 2 16:33:48 2009 Subject: [Histonet] HT licensing in 2011 In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3AFF@IS-E2K3.grhs.net> Is this a state bill, a federal bill or what. I have never heard of this. And are these "specified requirements" also part of the bill? Where can we read more about this bill? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, April 02, 2009 3:35 PM To: Laurie Colbert Cc: histonet-bounces@lists.utsouthwestern.edu; histonet Subject: Re: [Histonet] HT licensing in 2011 Laurie, The bill that everyone has been "quoting" is AB 2156. It does not state that histotechnicians will have to be licensed. The ASCP has specific eligibility requirements for HT certification and at this point they are not related to licensure in any state. Other states that have licensure do not just accept payment for the license. They require some form of competency exam. If a person is already ASCP certified than in some states they will be granted licensure. This is an excerpt from the bill. Under existing law, unlicensed laboratory personnel are authorized to perform specified activities, in a licensed clinical laboratory, under the direct and constant supervision of a physician and surgeon or another licensed person if certain criteria are met. Existing law authorizes these unlicensed laboratory personnel to perform specimen labeling, handling, preservation or fixation, processing or preparation, transportation, and storing. This bill would authorize a certified pathologists' assistant, within the specialty of pathology, demonstrating specified competency, to perform specified activities under the supervision and control of a pathologist. The bill would authorize a pathologists' assistant, a histologic technician, and a histotechnologist to prepare human surgical specimens for gross description and dissection under the direct supervision, as defined, of a qualified pathologist, if he or she meets specified requirements. The bill would authorize the department, on and after January 1, 2011, to adopt regulations establishing additional qualification requirements to perform the duties specified in these provisions. "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/02/2009 12:38 PM To "histonet" cc Subject [Histonet] HT licensing in 2011 Does anyone know about a law that is supposed to go into effect in 2011 that will require histotechs to be licensed? I also heard something about a person being eligible to take the HT exam if they get a state license - which can be obtained by simply paying a fee if they have 5 years of work experience? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmlaud <@t> gmail.com Thu Apr 2 17:40:18 2009 From: dmlaud <@t> gmail.com (Damien) Date: Thu Apr 2 17:40:21 2009 Subject: [Histonet] Re: Insect/Arthropod histotechnology Message-ID: <6f5a847e0904021540m7e8991b1x5f9aad583b631c97@mail.gmail.com> Hi Ana, I work with insects and related organisms. What did you need to know? -Damien Laudier From bernieb <@t> duke.edu Thu Apr 2 20:08:11 2009 From: bernieb <@t> duke.edu (Bernie Ball) Date: Thu Apr 2 20:08:16 2009 Subject: [Histonet] Tissue-Tek II Manual Message-ID: Does anyone have a copy of the Tissue-Tek II manual that they would be willing to share? (PDF preferred, hard-copy graciously accepted) Thanks (in advance), Bernie Ball Duke University A hat should be taken off when you greet a lady and left off for the rest of your life. Nothing looks more stupid than a hat. P.J. O'Rourke From Tony_Reilly <@t> health.qld.gov.au Thu Apr 2 21:09:32 2009 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Thu Apr 2 21:10:13 2009 Subject: [Histonet] Strange circles in IHC slides In-Reply-To: <49D4E3E2.2010708@vneubert.com> References: <49D4E3E2.2010708@vneubert.com> Message-ID: <49D5FC7B.471C.0039.0@health.qld.gov.au> Hi Looking at the images this appears to be the prozone effect which is caused by the primary antibody concentration being too high and has been well reported in both immunohistochemistry, immunology and serology articles. Do a google serch for prozone phenomenon and you will find an explanation of the theory involved. The classic feature are the circular areas which are unstained and this is reinforced in the images by the amount of background staining in the areas that are stained. Try titrating your primary out and I am sure will eliminate the problem. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> "V. Neubert" 3/04/2009 2:12 am >>> Hello, I really have some real links to real pictures of real relevance of the real histonet list. Really promised. http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg So, has ever anyone experienced sth. like this? My conjugate control (every step except the antibody) was fine, nothing to be seen about DAB and no circles at all. I used Shandon single-use coverplates, sterile buffer, fresh antibody aliquots. Any idea? Thanks, V. Neubert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From d.a.faichney <@t> stir.ac.uk Fri Apr 3 04:31:40 2009 From: d.a.faichney <@t> stir.ac.uk (Deborah Faichney) Date: Fri Apr 3 04:31:52 2009 Subject: [Histonet] marine animals and insects/ arthrops histotechnology In-Reply-To: References: Message-ID: <8ED3F2CA5B78E142B8193376C57330F8E1973BE542@EXCH2007.ad.stir.ac.uk> Hi Ana, Yes, I work for an aquaculture veterinary pathology lab and have experience with some of these, but particularly fish. Please feel free to contact me for more information, I'll help if I can. Debbie Faichney Histopathology Institute of aquaculture University of Stirling Stirling Scotland UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ana Resendes Sent: 02 April 2009 19:40 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marine animals and insects/ arthrops histotechnology Anyone out there expertize or with a good background or working experience in marine (fish, algae, shell moluscles crustaceans) and insects or arthropds histotechnology ? Thank you. Ana -- Ana Resendes DVM, MSc, PhD Veterinary Pathologist Centro de Investiga??o de Recursos Naturais (CIRN) Sec??o de Anatomia e Taxonomia Zool?gicas, Departamento de Biologia, Universidade dos A?ores. Rua da M?e de Deus, 58 - Apartado 1422 P - 9501-801 Ponta Delgada (A?ores) Portugal Tel. (+351) 296 650 000 ext. 1109 Fax (+351) 296 650 100 http://www.uac.pt/~pherg/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Academic Excellence at the Heart of Scotland. The University of Stirling is a charity registered in Scotland, number SC 011159. From tbraud <@t> holyredeemer.com Fri Apr 3 07:42:19 2009 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Apr 3 07:42:23 2009 Subject: [Histonet] RE: Histonet Digest, Vol 65, Issue 7 In-Reply-To: <4ad895dd0002c2fa@HolyRedeemer.com> Message-ID: Hello Histonetters, I understand most of the professionals on this website are histology professionals, however, I thought I would give it a try since I have not found a cytology listserv yet. Does anyone know how many slides per day a cytotechnologist would screen within a private lab setting (On average)? Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 Hi Alyssa - Get ready...here is the dirt CAP follows CLIA-88, and both are quite clear on limits for Cytotech screening. They are "no more than 100 slides in 24 hours" and include gyn and non-gyn including "new routine slides, 10% rescreen slides, and 5 year look back negative slides" "the maximum workload of 100 slides can be completed in no less than an 8-hour workday. This workload can also be expressed as slides per hour and is 12.5 slides/hour. These total limits apply regardless of the number of laboratories in which an individual works on a given day." Wait! it gets better.... "For primary screening of gyn liquid based preps" (read as Thin Prep) "each slide must be counted as a single slide for the purpose of workload recording." "For primary screening of non-gyn liquid based preps" (read as Thin Prep) "each slide may be counted as one-half slide for the purpose of workload recording, provided that the cells are dispersed over one-half or less of the total available slide area." Wait! there's still more... "Workload calculations may vary with the use of automated screening instruments. Laboratories should follow manufacturers instructions for workload calculations and must assure that CLIAA-88 requirements are fulfilled." and just when you thought it was safe.... "The laboratory director must establish the maximum workload (based on the capability/documented performance evaluation) for each individual examining slides and the limit must be reassessed at least every 6 months." The regulations go on to explain how the reassessment is to be done...blah, blah, blah.... Though my background and education are in Histology, I've served as technical supervisor over cytology departments for over 14 years, and the above listed %#@&! is why Cytology can drive you up the wall. Our cytotech's, when we had them, screened no more than 80 slides/day, when all they did was screen. No pulling slides, no running up to do an FNA....just sitting non-stop and screening slides. UGH! Sorry Histo folk - but some of you are probably in the same position as I am, so please feel free to chime in. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From bernieb <@t> duke.edu Fri Apr 3 08:06:13 2009 From: bernieb <@t> duke.edu (Bernie Ball) Date: Fri Apr 3 08:06:18 2009 Subject: [Histonet] Tissue-Tek II Manual In-Reply-To: References: Message-ID: <65497618-B795-4E3B-8C67-6446197BE47E@duke.edu> Updated request with model number. Does anyone have a copy of the Tissue-Tek II manual that they would be willing to share? (PDF preferred, hard-copy graciously accepted) One for a model 4553 Cryostat (apologies for not including that in the original post) Thanks (in advance), Bernie Ball Duke University From rjbuesa <@t> yahoo.com Fri Apr 3 08:27:18 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 3 08:27:23 2009 Subject: [Histonet] RE: Histonet Digest, Vol 65, Issue 7 In-Reply-To: Message-ID: <669698.40696.qm@web65714.mail.ac4.yahoo.com> Terri: Any private setting (including large reference labs) is ruled by the same standards as any other one. The screener threshold (when you need to hire an additional one) is 7,000 slides/year. Under separate cover I am sending you an article I wrote about the Cytology work within the Histology lab. Ren? J. --- On Fri, 4/3/09, Terri Braud wrote: From: Terri Braud Subject: [Histonet] RE: Histonet Digest, Vol 65, Issue 7 To: histonet@lists.utsouthwestern.edu Date: Friday, April 3, 2009, 8:42 AM Hello Histonetters, I understand most of the professionals on this website are histology professionals, however, I thought I would give it a try since I have not found a cytology listserv yet. Does anyone know how many slides per day a cytotechnologist would screen within a private lab setting (On average)? Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 Hi Alyssa - Get ready...here is the dirt CAP follows CLIA-88, and both are quite clear on limits for Cytotech screening. They are "no more than 100 slides in 24 hours" and include gyn and non-gyn including "new routine slides, 10% rescreen slides, and 5 year look back negative slides" "the maximum workload of 100 slides can be completed in no less than an 8-hour workday. This workload can also be expressed as slides per hour and is 12.5 slides/hour. These total limits apply regardless of the number of laboratories in which an individual works on a given day." Wait! it gets better.... "For primary screening of gyn liquid based preps" (read as Thin Prep) "each slide must be counted as a single slide for the purpose of workload recording." "For primary screening of non-gyn liquid based preps" (read as Thin Prep) "each slide may be counted as one-half slide for the purpose of workload recording, provided that the cells are dispersed over one-half or less of the total available slide area." Wait! there's still more... "Workload calculations may vary with the use of automated screening instruments. Laboratories should follow manufacturers instructions for workload calculations and must assure that CLIAA-88 requirements are fulfilled." and just when you thought it was safe.... "The laboratory director must establish the maximum workload (based on the capability/documented performance evaluation) for each individual examining slides and the limit must be reassessed at least every 6 months." The regulations go on to explain how the reassessment is to be done...blah, blah, blah.... Though my background and education are in Histology, I've served as technical supervisor over cytology departments for over 14 years, and the above listed %#@&! is why Cytology can drive you up the wall. Our cytotech's, when we had them, screened no more than 80 slides/day, when all they did was screen. No pulling slides, no running up to do an FNA....just sitting non-stop and screening slides. UGH! Sorry Histo folk - but some of you are probably in the same position as I am, so please feel free to chime in. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tgenade <@t> gmail.com Fri Apr 3 09:49:55 2009 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Fri Apr 3 09:49:59 2009 Subject: [Histonet] keratinocyte marker Message-ID: Hi all, Can anyone suggest a evolutionary conserved keratinocyte marker? I'm working with a fish (not zebrafish!) and need an antibody marker that can ID keratinocytes. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@freeshell.org tel: +27-84-632-1925 (c) ******************************************************************************** "For there is one God, and there is one mediator between God and men, the man Christ Jesus, who gave himself as a ransom for all." 1 Timothy 2:5-6 From SwainFrancesL <@t> uams.edu Fri Apr 3 10:35:09 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Fri Apr 3 10:35:58 2009 Subject: [Histonet] unsubscribe Message-ID: Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tifei <@t> foxmail.com Fri Apr 3 10:43:03 2009 From: tifei <@t> foxmail.com (TF) Date: Fri Apr 3 10:43:21 2009 Subject: [Histonet] Cryostat sections mounting with 0.1M PB solution onto slides References: Message-ID: <200904032342579533934@foxmail.com> After we cut the brain sections on a cryostat (OCT embedded), we brush a drop of 0.1M PB on to the slide before mounting the sections. Do anyone know the side effect of this - for example, the sections will peel off during immunostaining? Another question is about the dry time after mounting and before staining. I know some people asked similar questions before, but they are using fresh tissue for frozen sections. We here perfuse the animal, post-fix the tissue and sink it in sucrose before making cryostat sections. So we may dry the sections in air up to weeks. I would like to ask about the proper heating time (with a heat plate) before dry the sections up in a fumehood. Should we heat the sections at 60 degree for 10 min or 2 hours shortly after mounting? Thanks very much. 2009-04-03 TF From kmerriam2003 <@t> yahoo.com Fri Apr 3 10:47:39 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Apr 3 10:47:43 2009 Subject: [Histonet] Strange circles in IHC slides In-Reply-To: <49D4E3E2.2010708@vneubert.com> References: <49D4E3E2.2010708@vneubert.com> Message-ID: <534413.42568.qm@web50301.mail.re2.yahoo.com> I use this system quite often, when doing manual IHC.??I believe that these are?air bubbles that were in the buffer solution that you used when you first put the slides into the cytoclips; I usually let the buffer settle a?little bit in the beaker before I load my slides (to allow time for any bubbles to pop).? Then I put?about 1-2 mls of buffer (with detergent) at the bottom of the clip and slowly put the slide onto the clip?(just like coverslipping); I always make sure that there are no visible bubbles when loading the slides (again, just like coverslipping).? Then I use an eppendorf repeat-pipettor (set at 2-mls) for my washes (again, with buffer?+ detergent). ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: V. Neubert To: histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2009 12:12:18 PM Subject: [Histonet] Strange circles in IHC slides Hello, I really have some real links to real pictures of real relevance of the real histonet list. Really promised. http://img12.imageshack.us/img12/8513/ts0402162049.jpg http://img13.imageshack.us/img13/6514/ts0402162104.jpg So, has ever anyone experienced sth. like this? My conjugate control (every step except the antibody) was fine, nothing to be seen about DAB and no circles at all. I used Shandon single-use coverplates, sterile buffer, fresh antibody aliquots. Any idea? Thanks, V. Neubert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Fri Apr 3 10:47:33 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Apr 3 10:48:51 2009 Subject: [Histonet] unsubscribe In-Reply-To: References: Message-ID: <49D62F95.7020604@umdnj.edu> Since it's Friday, does that mean that I can once again whine about my "unsubscribe me!!!" pet-peeve, despite this week's earlier ugliness on the list? ;) Swain, Frances L wrote: > Frances L. Swain HT(ASCP) A. A. S. > Special Procedures Technician > Department of Orthopaedic Surgery > Center for Orthopaedic Research > Barton Research Building 2R28 > 4301 West Markham Street > Little Rock AR 72205 > (501) 686-8739 PHONE > (501) 686-8987 FAX > swainfrancesl@uams.edu email > > > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From jmcgough <@t> clinlab.com Fri Apr 3 11:57:15 2009 From: jmcgough <@t> clinlab.com (Jason McGough) Date: Fri Apr 3 11:57:22 2009 Subject: [Histonet] Protein Block In-Reply-To: <534413.42568.qm@web50301.mail.re2.yahoo.com> Message-ID: We recently purchased a Dako Autostainer with the PT Link for our IHC's. We are experiencing more background staining then before when we had the older version of the Dako Autostainer. Does anybody use protein block (serum free) to help minimize background staining? Thank you for your replies. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com From TJJ <@t> stowers.org Fri Apr 3 12:17:34 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Fri Apr 3 12:18:08 2009 Subject: [Histonet] Re: Strange Circles in IHC slides Message-ID: The circles could be either from air bubbles present when you mount the slides onto the coverplates, or they could have arisen after exposure to H2O2 as part of the staining procedure. Doing the H2O2 off instrument in a coplin jar and then rinsing and mounting them in the coverplates will help keep that from happening. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From MadaryJ <@t> MedImmune.com Fri Apr 3 12:28:12 2009 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Fri Apr 3 12:28:17 2009 Subject: [Histonet] Histo license Message-ID: I have a license to drive a car, and a certification that allows me to pay each year to ASCP. I feel so credentialed! I think we should all take Saturday and Sunday off. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory Mgr One Medimmune Way, Lab 2438-Area 4 Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. > P Please consider the environment before printing this e-mail > To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From Charlene.Henry <@t> STJUDE.ORG Fri Apr 3 12:35:14 2009 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri Apr 3 12:35:19 2009 Subject: [Histonet] Tonsil Control Blocks Needed Message-ID: <03E1F5968F60C5448635D49D38B283ED027DDD741C@SJMEMXMBS11.stjude.sjcrh.local> Fellow Histonetters, I am having an extremely hard time obtaining tonsil control tissue and was wondering if anyone would have some they could share. I would be glad to exchange some rhabdomyosarcoma tissue blocks in exchange. Please let me know if you can help. Thanks, Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105 901-595-3191 fax 901-595-3100 ________________________________ Email Disclaimer: www.stjude.org/emaildisclaimer From rosenfeldtek <@t> hotmail.com Fri Apr 3 12:38:58 2009 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Fri Apr 3 12:39:03 2009 Subject: [Histonet] RE: Tonsil Control Blocks Needed In-Reply-To: <03E1F5968F60C5448635D49D38B283ED027DDD741C@SJMEMXMBS11.stjude.sjcrh.local> References: <03E1F5968F60C5448635D49D38B283ED027DDD741C@SJMEMXMBS11.stjude.sjcrh.local> Message-ID: I used to buy tonsil slides from Dako--I used them as a positive control for PCNA. Jerry Ricks Research Scientist University of Washington Department of Pathology > From: Charlene.Henry@STJUDE.ORG > To: histonet@lists.utsouthwestern.edu > Date: Fri, 3 Apr 2009 12:35:14 -0500 > Subject: [Histonet] Tonsil Control Blocks Needed > > Fellow Histonetters, > I am having an extremely hard time obtaining tonsil control tissue and was wondering if anyone would have some they could share. I would be glad to exchange some rhabdomyosarcoma tissue blocks in exchange. Please let me know if you can help. > Thanks, > > Charlene Henry HT (ASCP), QIHC > Anatomic Pathology Section Head > Department of Pathology > St. Jude Children's Research Hospital > 262 Danny Thomas Place > Memphis, TN 38105 > 901-595-3191 > fax 901-595-3100 > > > ________________________________ > Email Disclaimer: www.stjude.org/emaildisclaimer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Rediscover Hotmail?: Get quick friend updates right in your inbox. http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Updates1_042009 From jrobertson <@t> pathologysciences.com Fri Apr 3 12:47:25 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Fri Apr 3 12:47:32 2009 Subject: [Histonet] Tonsil Control Blocks Needed In-Reply-To: <03E1F5968F60C5448635D49D38B283ED027DDD741C@SJMEMXMBS11.stjude.sjcrh.local> References: <03E1F5968F60C5448635D49D38B283ED027DDD741C@SJMEMXMBS11.stjude.sjcrh.local> Message-ID: <518CD6920AA7154193CBE5977CD88073177F36@psmgsrv2.PSMG.local> Contact me off line and I'd be happy to see what we can do to accommodate you at jrobertson@pathologysciences.com Thank you. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Histology Day Supervisor 183 E. 8th Ave. Chico, CA 95926 530-891-6244 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Henry, Charlene Sent: Friday, April 03, 2009 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tonsil Control Blocks Needed Fellow Histonetters, I am having an extremely hard time obtaining tonsil control tissue and was wondering if anyone would have some they could share. I would be glad to exchange some rhabdomyosarcoma tissue blocks in exchange. Please let me know if you can help. Thanks, Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105 901-595-3191 fax 901-595-3100 ________________________________ Email Disclaimer: www.stjude.org/emaildisclaimer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Fri Apr 3 13:00:20 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Apr 3 13:00:29 2009 Subject: [Histonet] Removing PMMA blocks from glass vials Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CAB@LSRIEXCH1.lsmaster.lifespan.org> Someone gave me some small PMMA blocks polymerized inside glass scintillation vials. I always use plastic vials. Obviously the vials have to be broken to get the blocks out. Does anyone have some advice on the best way to do this? The guy who brought them said he thought I could "use liquid nitrogen", but had no specifics. Any help will be appreciated. From Shakun.Aswani <@t> acologix.com Fri Apr 3 13:23:30 2009 From: Shakun.Aswani <@t> acologix.com (Shakun Aswani) Date: Fri Apr 3 13:23:36 2009 Subject: [Histonet] Removing PMMA blocks from glass vials In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835CAB@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E03835CAB@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <777AB0DE519C8E46A6220E2287C5BAD301B346B9@EXCHANGE.acologix.com> Hi Paul, I did so many of those PMMA blocks cracking. Take a under pad, line your blocks not too many and cover with another under pad and start (literally) hammering them one by one with hammer gently I am not kidding. Make sure you wear safety glasses and double gloves to protect your hands. When the blocks are cracked remove them and rinse with tap water and small glass pieces will go into sharp container. I know seems very complicated but it is not. If you have more questions regarding this please fell free to contact me. Good Luck Shakun -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, April 03, 2009 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing PMMA blocks from glass vials Someone gave me some small PMMA blocks polymerized inside glass scintillation vials. I always use plastic vials. Obviously the vials have to be broken to get the blocks out. Does anyone have some advice on the best way to do this? The guy who brought them said he thought I could "use liquid nitrogen", but had no specifics. Any help will be appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Fri Apr 3 13:34:08 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Fri Apr 3 13:34:11 2009 Subject: [Histonet] Removing PMMA blocks from glass vials In-Reply-To: <777AB0DE519C8E46A6220E2287C5BAD301B346B9@EXCHANGE.acologix.com> Message-ID: <1439078926.1521651238783648027.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I agree.? It is a little messy and can be dangerous if you are not paying enough attention.? LN just gets the glass cold and cracks it so you don't need a hammer.? If you leave it in the LN too long the block may crack with the vial. Pam Marcum UPENN Vet School New Bolton Center ----- Original Message ----- From: "Shakun Aswani" To: "Paul Monfils" , histonet@lists.utsouthwestern.edu Sent: Friday, April 3, 2009 2:23:30 PM GMT -05:00 US/Canada Eastern Subject: RE: [Histonet] Removing PMMA blocks from glass vials Hi Paul, I did so many of those PMMA blocks cracking. Take a under pad, line your blocks not too many and cover with another under pad and start (literally) hammering them one by one with hammer gently I am not kidding. Make sure you wear safety glasses and double gloves to protect your hands. When the blocks are cracked remove them and rinse with tap water and small glass pieces will go into sharp container. I know seems very complicated but it is not. If you have more questions regarding this please fell free to contact me. Good Luck Shakun -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, April 03, 2009 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing PMMA blocks from glass vials Someone gave me some small PMMA blocks polymerized inside glass scintillation vials. ?I always use plastic vials. ?Obviously the vials have to be broken to get the blocks out. ?Does anyone have some advice on the best way to do this? ?The guy who brought them said he thought I could "use liquid nitrogen", but had no specifics. ?Any help will be appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Fri Apr 3 14:09:34 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Fri Apr 3 14:09:53 2009 Subject: [Histonet] Entamoeba Trophozoites Message-ID: <49D626AE020000EE00027F0A@smtp-gw.hurleymc.com> I need help! Just received a needle biopsy of liver submitted in normal saline. They did all the routine cultures/ fungal, and are requesting a wet mount on this tissue for Entamoeba Trophozoites. I have contacted all of my resources in and out of state, and they cannot help me. HELP!! thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 From robert_schoonhoven <@t> yahoo.com Fri Apr 3 14:10:05 2009 From: robert_schoonhoven <@t> yahoo.com (Robert Schoonhoven) Date: Fri Apr 3 14:10:07 2009 Subject: [Histonet] Removing PMMA blocks from glass vials In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835CAB@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E03835CAB@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <354336.50493.qm@web31107.mail.mud.yahoo.com> Paul, Put in LN2 (or leave in -80 freezer for about 1 hour). Using the appropriate PPE, wrap in towel, hit with Hammer (gently...), welcome to use this SOP. Bob, Robert Schoonhoven ________________________________ From: "Monfils, Paul" To: histonet@lists.utsouthwestern.edu Sent: Friday, April 3, 2009 2:00:20 PM Subject: [Histonet] Removing PMMA blocks from glass vials Someone gave me some small PMMA blocks polymerized inside glass scintillation vials. I always use plastic vials. Obviously the vials have to be broken to get the blocks out. Does anyone have some advice on the best way to do this? The guy who brought them said he thought I could "use liquid nitrogen", but had no specifics. Any help will be appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SwainFrancesL <@t> uams.edu Fri Apr 3 14:09:09 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Fri Apr 3 14:10:12 2009 Subject: [Histonet] RE: Removing PMMA blocks from glass vials In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835CAB@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E03835CAB@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: I would not use liquid nitrogen. The safest way to do this is to put the blocks in a refrigerator freezer for a couple of hours. Remove quickly, remove caps, wrap in paper towels and hit with a hammer. Be sure to wear protective clothing, eye covers and gloves. When the glass has broken into the towel put the towel in a sharps container, rinse the block and procees with the sectioning. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, April 03, 2009 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing PMMA blocks from glass vials Someone gave me some small PMMA blocks polymerized inside glass scintillation vials. I always use plastic vials. Obviously the vials have to be broken to get the blocks out. Does anyone have some advice on the best way to do this? The guy who brought them said he thought I could "use liquid nitrogen", but had no specifics. Any help will be appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Fri Apr 3 15:12:15 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 3 15:12:19 2009 Subject: [Histonet] Entamoeba Trophozoites In-Reply-To: <49D626AE020000EE00027F0A@smtp-gw.hurleymc.com> Message-ID: <635986.93921.qm@web65713.mail.ac4.yahoo.com> Lynette: Since it was received in saline and the tissue has its normal consistency I would prepare a squash (squash the tissue between 2 glass slides not between coverslips) with a rotary movement so you have 2 large areas of squashed tissue. After that, air dry,?fix with methanol, air dry again and stain with Giemsa. That is what I would do. Try it if you do not have a better option. Ren? J. --- On Fri, 4/3/09, Lynette Pavelich wrote: From: Lynette Pavelich Subject: [Histonet] Entamoeba Trophozoites To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Friday, April 3, 2009, 3:09 PM I need help! Just received a needle biopsy of liver submitted in normal saline. They did all the routine cultures/ fungal, and are requesting a wet mount on this tissue for Entamoeba Trophozoites. I have contacted all of my resources in and out of state, and they cannot help me. HELP!! thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Fri Apr 3 15:11:27 2009 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Fri Apr 3 15:13:43 2009 Subject: [Histonet] RE: Removing PMMA blocks from glass vials In-Reply-To: References: <4EBFF65383B74D49995298C4976D1D5E03835CAB@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <49D66D6F.3090007@umdnj.edu> yup, i used to give them a quick dip in liquid nitrogen then whack them with the edge of a metal spatula. under the hood, of course, with a thick layer of glass between my face/body and the flying glass shards ;) Swain, Frances L wrote: > I would not use liquid nitrogen. The safest way to do this is to put the blocks in a refrigerator freezer for a couple of hours. Remove quickly, remove caps, wrap in paper towels and hit with a hammer. Be sure to wear protective clothing, eye covers and gloves. When the glass has broken into the towel put the towel in a sharps container, rinse the block and procees with the sectioning. > > Frances L. Swain HT(ASCP) A. A. S. > Special Procedures Technician > Department of Orthopaedic Surgery > Center for Orthopaedic Research > Barton Research Building 2R28 > 4301 West Markham Street > Little Rock AR 72205 > (501) 686-8739 PHONE > (501) 686-8987 FAX > swainfrancesl@uams.edu email > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul > Sent: Friday, April 03, 2009 1:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Removing PMMA blocks from glass vials > > Someone gave me some small PMMA blocks polymerized inside glass scintillation vials. I always use plastic vials. Obviously the vials have to be broken to get the blocks out. Does anyone have some advice on the best way to do this? The guy who brought them said he thought I could "use liquid nitrogen", but had no specifics. Any help will be appreciated. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From pjfnefro <@t> duke.edu Fri Apr 3 16:33:54 2009 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Fri Apr 3 16:34:05 2009 Subject: [Histonet] Removing PMMA blocks from glass vials In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835CAB@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E03835CAB@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: OK, silly question time: How do you get them out of *plastic* vials? I've always used the glass scint vials,too. Doesn't the PMMA melt plastic vials (or stick too well to them)? -Pat Flannery Duke Med On Apr 3, 2009, at 2:00 PM, "Monfils, Paul" wrote: > Someone gave me some small PMMA blocks polymerized inside glass > scintillation vials. I always use plastic vials. Obviously the > vials have to be broken to get the blocks out. Does anyone have > some advice on the best way to do this? The guy who brought them > said he thought I could "use liquid nitrogen", but had no > specifics. Any help will be appreciated. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Fri Apr 3 16:47:04 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Apr 3 16:47:04 2009 Subject: [Histonet] Removing PMMA blocks from glass vials In-Reply-To: References: <4EBFF65383B74D49995298C4976D1D5E03835CAB@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: You need to first make sure you are using polypropylene vials.....the polyethylene vials will melt when using MMA. Next, use a box knife to score the sides and pop out the block. Larger plastic molds can be re- used if you tap out the block by beating it on a counter top. Jack On Apr 3, 2009, at 4:33 PM, Pat Flannery wrote: > OK, silly question time: How do you get them out of *plastic* > vials? I've always used the glass scint vials,too. Doesn't the PMMA > melt plastic vials (or stick too well to them)? > > -Pat Flannery > Duke Med > > On Apr 3, 2009, at 2:00 PM, "Monfils, Paul" > wrote: > >> Someone gave me some small PMMA blocks polymerized inside glass >> scintillation vials. I always use plastic vials. Obviously the >> vials have to be broken to get the blocks out. Does anyone have >> some advice on the best way to do this? The guy who brought them >> said he thought I could "use liquid nitrogen", but had no >> specifics. Any help will be appreciated. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jhabecke <@t> fhcrc.org Fri Apr 3 17:54:12 2009 From: jhabecke <@t> fhcrc.org (Randolph-Habecker, Julie) Date: Fri Apr 3 17:54:19 2009 Subject: [Histonet] Problems with mouse brains Message-ID: <040346FA7309BD439C327F97D4C4D69B04A1C481@ISIS.fhcrc.org> Folks, I need some input on a problem we're seeing with some mouse brain samples. The samples are from new born mice P0 to P14 which have been fixed in 10% NBF (Fisher brand and well within expiration date) for 72 hours. They are then transferred to 70% etoh and processed on an 8 hour process. After the tissue is embedded, we are taking sagital sections, drying them overnight, and then baking overnight at 60C. They are then stained with H&E. This has been working great but now all of a sudden we have very light H&E staining, nuclear bubbling, and extensive tissue cracking. I also have noticed some formalin pigment. My first thought is that there was difference in formalin or time in formalin but that is not the case. I also wondered if the tissue might be exposed to excessive heat. We checked all of the temperatures in our processor, embedding center, and water baths - all were within tolerance. Any ideas what might cause all three artifacts? Could it be from inadequate paraffin infiltration? Any help would be greatly appreciated! Thanks, Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org From lori.garcia <@t> medtronic.com Fri Apr 3 18:12:00 2009 From: lori.garcia <@t> medtronic.com (Garcia, Lori, Sr. Scientist) Date: Fri Apr 3 18:12:06 2009 Subject: [Histonet] Technovit 7200 (isobornylmethacrylate) staining Message-ID: <0F844FC5FD7212428416B8565B47FA0202AD9293@STSM1BMSGM03.ent.core.medtronic.com> This question is specifically for Linda Jenkins or anyone who has worked with Technovit 7200, I am trying to do an H&E stain on ~70 um thick ground sections on plastic slides. I have followed the Donath method all the way through processing, but the sections do not pick up stains the way the booklet says they should. I am even having trouble following GMA staining procedures that I have found, in fact am afraid to try too many because the acrylic forms tiny cracks that interfere with microscopy. I have researched the histonet archives, but haven't found anything other than a couple of similar inquiries. I would very much appreciate any advice from people who have worked with this specific formulation. Happy Friday! Lori [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From ratliffjack <@t> hotmail.com Fri Apr 3 18:30:20 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Apr 3 18:30:19 2009 Subject: [Histonet] Technovit 7200 (isobornylmethacrylate) staining In-Reply-To: <0F844FC5FD7212428416B8565B47FA0202AD9293@STSM1BMSGM03.ent.core.medtronic.com> References: <0F844FC5FD7212428416B8565B47FA0202AD9293@STSM1BMSGM03.ent.core.medtronic.com> Message-ID: To my knowledge it is near impossible to obtain an adequate H&E stain on thick resin sections. If this is not true, I would be interested in the same information. Jack On Apr 3, 2009, at 6:12 PM, "Garcia, Lori, Sr. Scientist" wrote: > This question is specifically for Linda Jenkins or anyone who has > worked > with Technovit 7200, > > I am trying to do an H&E stain on ~70 um thick ground sections on > plastic slides. I have followed the Donath method all the way through > processing, but the sections do not pick up stains the way the booklet > says they should. I am even having trouble following GMA staining > procedures that I have found, in fact am afraid to try too many > because > the acrylic forms tiny cracks that interfere with microscopy. I have > researched the histonet archives, but haven't found anything other > than > a couple of similar inquiries. > > I would very much appreciate any advice from people who have worked > with > this specific formulation. > > Happy Friday! > > Lori > > [CONFIDENTIALITY AND PRIVACY NOTICE] > > Information transmitted by this email is proprietary to Medtronic > and is intended for use only by the individual or entity to which it > is addressed, and may contain information that is private, > privileged, confidential or exempt from disclosure under applicable > law. If you are not the intended recipient or it appears that this > mail has been forwarded to you without proper authority, you are > notified that any use or dissemination of this information in any > manner is strictly prohibited. In such cases, please delete this > mail from your records. > > To view this notice in other languages you can either select the > following link or manually copy and paste the link into the address > bar of a web browser: http://emaildisclaimer.medtronic.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bernietaupin <@t> ymail.com Sat Apr 4 00:21:25 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Sat Apr 4 00:21:29 2009 Subject: [Histonet] Entamoeba Trophozoites In-Reply-To: <635986.93921.qm@web65713.mail.ac4.yahoo.com> References: <635986.93921.qm@web65713.mail.ac4.yahoo.com> Message-ID: <511036.54656.qm@web43504.mail.sp1.yahoo.com> Anyone else think this Rene fella comes across as a real know-it-all? ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Lynette Pavelich Sent: Friday, April 3, 2009 4:12:15 PM Subject: [Histonet] Entamoeba Trophozoites Lynette: Since it was received in saline and the tissue has its normal consistency I would prepare a squash (squash the tissue between 2 glass slides not between coverslips) with a rotary movement so you have 2 large areas of squashed tissue. After that, air dry, fix with methanol, air dry again and stain with Giemsa. That is what I would do. Try it if you do not have a better option. Ren? J. --- On Fri, 4/3/09, Lynette Pavelich wrote: From: Lynette Pavelich Subject: [Histonet] Entamoeba Trophozoites To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Friday, April 3, 2009, 3:09 PM I need help! Just received a needle biopsy of liver submitted in normal saline. They did all the routine cultures/ fungal, and are requesting a wet mount on this tissue for Entamoeba Trophozoites. I have contacted all of my resources in and out of state, and they cannot help me. HELP!! thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Sat Apr 4 08:15:02 2009 From: kemlo <@t> f2s.com (kemlo) Date: Sat Apr 4 08:15:18 2009 Subject: [Histonet] Entamoeba Trophozoites In-Reply-To: <511036.54656.qm@web43504.mail.sp1.yahoo.com> References: <635986.93921.qm@web65713.mail.ac4.yahoo.com> <511036.54656.qm@web43504.mail.sp1.yahoo.com> Message-ID: <9ED35C9D2C5B4AFB834241AC979BDEA0@KemloPC> Is there a problem with that sonny? Maybe you ought to get the gender right before you attack someone who tries very hard to help people. Ignorant people, such as you, usually attack those brighter than themselves. The Middle Ages beckons you, sonny! Kemlo Rogerson Pathology Manager used to dealing with half wits (comes with the territory). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: 04 April 2009 06:21 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Entamoeba Trophozoites Anyone else think this Rene fella comes across as a real know-it-all? ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Lynette Pavelich Sent: Friday, April 3, 2009 4:12:15 PM Subject: [Histonet] Entamoeba Trophozoites Lynette: Since it was received in saline and the tissue has its normal consistency I would prepare a squash (squash the tissue between 2 glass slides not between coverslips) with a rotary movement so you have 2 large areas of squashed tissue. After that, air dry, fix with methanol, air dry again and stain with Giemsa. That is what I would do. Try it if you do not have a better option. Ren? J. --- On Fri, 4/3/09, Lynette Pavelich wrote: From: Lynette Pavelich Subject: [Histonet] Entamoeba Trophozoites To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Friday, April 3, 2009, 3:09 PM I need help! Just received a needle biopsy of liver submitted in normal saline. They did all the routine cultures/ fungal, and are requesting a wet mount on this tissue for Entamoeba Trophozoites. I have contacted all of my resources in and out of state, and they cannot help me. HELP!! thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From resendes.ana <@t> gmail.com Sat Apr 4 09:02:54 2009 From: resendes.ana <@t> gmail.com (Ana Resendes) Date: Sat Apr 4 09:03:00 2009 Subject: [Histonet] questions about permiabilisation and fixation of nematode larva for further immunohistochemistry Message-ID: Hello to the Histotects community worldwide. Would like to know if someone out there has any suggestion or have worked with nematode larva: 1- fixation and after (protocol) 2-permeabilization of the chitin outer membrane (protocol) in order to: 3- adhere them to slides (maybe polylisine slides??? any other suggestion or protocol for slide adhesion of larva) 4- Run a immunohistochemistry on them (suggestions or protocol for any adjustments needed for further permiabilization and antigen retrieval if needed) Thanks Ana -- Ana Resendes DVM, MSc, PhD Veterinary Pathologist Centro de Investiga??o de Recursos Naturais (CIRN) Sec??o de Anatomia e Taxonomia Zool?gicas, Departamento de Biologia, Universidade dos A?ores. Rua da M?e de Deus, 58 - Apartado 1422 P - 9501-801 Ponta Delgada (A?ores) Portugal Tel. (+351) 296 650 000 ext. 1109 Fax (+351) 296 650 100 http://www.uac.pt/~pherg/ From resendes.ana <@t> gmail.com Sat Apr 4 09:12:01 2009 From: resendes.ana <@t> gmail.com (Ana Resendes) Date: Sat Apr 4 09:12:07 2009 Subject: [Histonet] Problems with mouse brains In-Reply-To: <040346FA7309BD439C327F97D4C4D69B04A1C481@ISIS.fhcrc.org> References: <040346FA7309BD439C327F97D4C4D69B04A1C481@ISIS.fhcrc.org> Message-ID: 2009/4/3 Randolph-Habecker, Julie > Folks, > > I need some input on a problem we're seeing with some mouse brain > samples. The samples are from new born mice P0 to P14 which have been > fixed in 10% NBF (Fisher brand and well within expiration date) for 72 > hours. They are then transferred to 70% etoh and processed on an 8 hour > process. After the tissue is embedded, we are taking sagital sections, > drying them overnight, and then baking overnight at 60C. They are then > stained with H&E. This has been working great but now all of a sudden we > have very light H&E staining, nuclear bubbling, and extensive tissue > cracking. I also have noticed some formalin pigment. > > My first thought is that there was difference in formalin or time in > formalin but that is not the case. I also wondered if the tissue might > be exposed to excessive heat. We checked all of the temperatures in our > processor, embedding center, and water baths - all were within > tolerance. > > Any ideas what might cause all three artifacts? Could it be from > inadequate paraffin infiltration? > > Any help would be greatly appreciated! > > Thanks, > > Julie > > Julie Randolph-Habecker, Ph.D. > Staff Scientist - Director > Experimental Histopathology Shared Resource > Fred Hutchinson Cancer Research Center > 1100 Fairview Ave, N. DE-360 (Please note new location) > Seattle WA 98109-1024 > 206-667-6119 > jhabecke@fhcrc.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Ana Resendes DVM, MSc, PhD Veterinary Pathologist Centro de Investiga??o de Recursos Naturais (CIRN) Sec??o de Anatomia e Taxonomia Zool?gicas, Departamento de Biologia, Universidade dos A?ores. Rua da M?e de Deus, 58 - Apartado 1422 P - 9501-801 Ponta Delgada (A?ores) Portugal Tel. (+351) 296 650 000 ext. 1109 Fax (+351) 296 650 100 http://www.uac.pt/~pherg/ From resendes.ana <@t> gmail.com Sat Apr 4 09:15:45 2009 From: resendes.ana <@t> gmail.com (Ana Resendes) Date: Sat Apr 4 09:15:50 2009 Subject: [Histonet] Problems with mouse brains In-Reply-To: <040346FA7309BD439C327F97D4C4D69B04A1C481@ISIS.fhcrc.org> References: <040346FA7309BD439C327F97D4C4D69B04A1C481@ISIS.fhcrc.org> Message-ID: have very light H&E staining: first you should check if either your eosin or haematoxilin are over used nuclear bubbling: can be a lot of things but can be inclusion. Brain sections are big so make at least a 24 h inclusion process. and extensive tissue cracking: could be over heating or bad inclusion. You have to check if reagents in inclusors are ok or over passed, I would change all reagents. I also have noticed some formalin pigment: your formol is not well tamponated 2009/4/3 Randolph-Habecker, Julie > Folks, > > I need some input on a problem we're seeing with some mouse brain > samples. The samples are from new born mice P0 to P14 which have been > fixed in 10% NBF (Fisher brand and well within expiration date) for 72 > hours. They are then transferred to 70% etoh and processed on an 8 hour > process. After the tissue is embedded, we are taking sagital sections, > drying them overnight, and then baking overnight at 60C. They are then > stained with H&E. This has been working great but now all of a sudden we > have very light H&E staining, nuclear bubbling, and extensive tissue > cracking. I also have noticed some formalin pigment. > > My first thought is that there was difference in formalin or time in > formalin but that is not the case. I also wondered if the tissue might > be exposed to excessive heat. We checked all of the temperatures in our > processor, embedding center, and water baths - all were within > tolerance. > > Any ideas what might cause all three artifacts? Could it be from > inadequate paraffin infiltration? > > Any help would be greatly appreciated! > > Thanks, > > Julie > > Julie Randolph-Habecker, Ph.D. > Staff Scientist - Director > Experimental Histopathology Shared Resource > Fred Hutchinson Cancer Research Center > 1100 Fairview Ave, N. DE-360 (Please note new location) > Seattle WA 98109-1024 > 206-667-6119 > jhabecke@fhcrc.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Ana Resendes DVM, MSc, PhD Veterinary pathologist Centro de Investiga??o de Recursos Naturais (CIRN) Sec??o de Anatomia e Taxonomia Zool?gicas, Departamento de Biologia, Universidade dos A?ores. Rua da M?e de Deus, 58 - Apartado 1422 P - 9501-801 Ponta Delgada (A?ores) Portugal Tel. (+351) 296 650 000 ext. 1109 Fax (+351) 296 650 100 http://www.uac.pt/~pherg/ From resendes.ana <@t> gmail.com Sat Apr 4 09:19:18 2009 From: resendes.ana <@t> gmail.com (Ana Resendes) Date: Sat Apr 4 09:19:23 2009 Subject: [Histonet] Tonsil Control Blocks Needed In-Reply-To: <03E1F5968F60C5448635D49D38B283ED027DDD741C@SJMEMXMBS11.stjude.sjcrh.local> References: <03E1F5968F60C5448635D49D38B283ED027DDD741C@SJMEMXMBS11.stjude.sjcrh.local> Message-ID: I can provide nomal pig tonsils if they are usefull. Rhabdomyosarcoma block would be nice! 2009/4/3 Henry, Charlene > Fellow Histonetters, > I am having an extremely hard time obtaining tonsil control tissue and was > wondering if anyone would have some they could share. I would be glad to > exchange some rhabdomyosarcoma tissue blocks in exchange. Please let me know > if you can help. > Thanks, > > Charlene Henry HT (ASCP), QIHC > Anatomic Pathology Section Head > Department of Pathology > St. Jude Children's Research Hospital > 262 Danny Thomas Place > Memphis, TN 38105 > 901-595-3191 > fax 901-595-3100 > > > ________________________________ > Email Disclaimer: www.stjude.org/emaildisclaimer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Ana Resendes DVM, MSc, PhD Centro de Investiga??o de Recursos Naturais (CIRN) Sec??o de Anatomia e Taxonomia Zool?gicas, Departamento de Biologia, Universidade dos A?ores. Rua da M?e de Deus, 58 - Apartado 1422 P - 9501-801 Ponta Delgada (A?ores) Portugal Tel. (+351) 296 650 000 ext. 1109 Fax (+351) 296 650 100 http://www.uac.pt/~pherg/ From aj.taylor <@t> blueyonder.co.uk Sat Apr 4 11:42:44 2009 From: aj.taylor <@t> blueyonder.co.uk (alan taylor) Date: Sat Apr 4 11:42:49 2009 Subject: [Histonet] Nice one Kemlo Message-ID: <58F70F8C715A45D0AB2E64C806B81D43@merlin> Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. From rjbuesa <@t> yahoo.com Sat Apr 4 12:01:36 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Apr 4 12:01:40 2009 Subject: [Histonet] Nice one Kemlo In-Reply-To: <58F70F8C715A45D0AB2E64C806B81D43@merlin> Message-ID: <658345.87226.qm@web65701.mail.ac4.yahoo.com> Thanks to Kenlo and Alan?for your nice words, only a detail though: I am a HE and not a SHE?that just?turned 75 on April First, I am "a legitimate April Fool" and I intend to keep doing what I am doing for as long as I can. Ren? J. --- On Sat, 4/4/09, alan taylor wrote: From: alan taylor Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Sat Apr 4 13:28:30 2009 From: kemlo <@t> f2s.com (kemlo) Date: Sat Apr 4 13:28:42 2009 Subject: [Histonet] Nice one Kemlo In-Reply-To: <658345.87226.qm@web65701.mail.ac4.yahoo.com> References: <58F70F8C715A45D0AB2E64C806B81D43@merlin> <658345.87226.qm@web65701.mail.ac4.yahoo.com> Message-ID: I apologise over this error over your gender; maybe it's because you are so caring and the stereotype takes over. But I think anyone that takes you on must go through me, ok? Your student 38 years in Pathology and still learning sometimes from you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 04 April 2009 18:02 To: histonet@lists.utsouthwestern.edu; alan taylor Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan?for your nice words, only a detail though: I am a HE and not a SHE?that just?turned 75 on April First, I am "a legitimate April Fool" and I intend to keep doing what I am doing for as long as I can. Ren? J. --- On Sat, 4/4/09, alan taylor wrote: From: alan taylor Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Apr 4 14:01:46 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Apr 4 14:01:52 2009 Subject: [Histonet] Nice one Kemlo In-Reply-To: Message-ID: <773630.96046.qm@web65704.mail.ac4.yahoo.com> OK! Ren? J. --- On Sat, 4/4/09, kemlo wrote: From: kemlo Subject: RE: [Histonet] Nice one Kemlo To: rjbuesa@yahoo.com, histonet@lists.utsouthwestern.edu, "'alan taylor'" Date: Saturday, April 4, 2009, 2:28 PM I apologise over this error over your gender; maybe it's because you are so caring and the stereotype takes over. But I think anyone that takes you on must go through me, ok? Your student 38 years in Pathology and still learning sometimes from you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 04 April 2009 18:02 To: histonet@lists.utsouthwestern.edu; alan taylor Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan?for your nice words, only a detail though: I am a HE and not a SHE?that just?turned 75 on April First, I am "a legitimate April Fool" and I intend to keep doing what I am doing for as long as I can. Ren? J. --- On Sat, 4/4/09, alan taylor wrote: From: alan taylor Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aj.taylor <@t> blueyonder.co.uk Sat Apr 4 15:10:33 2009 From: aj.taylor <@t> blueyonder.co.uk (alan taylor) Date: Sat Apr 4 15:10:38 2009 Subject: [Histonet] Nice one Kemlo References: <773630.96046.qm@web65704.mail.ac4.yahoo.com> Message-ID: Rene. Sincerest apologies to all parties. But again, I'm with Kemlo on this one. It's that old clash of cultures again. Here in Western Europe 'Rene' would be almost singularly appropriate to the female gender, but as with so many first names they can be freely interchangable between both genders, leading to the confusion. Some of you may recall the great travel, cookery writer and chef Rene Cutforth who made such excellent TV programmes during the 60's & 70's for example. He made complicated French cuisine seem so easy to prepare. Enjoy your weekend. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon England ----- Original Message ----- From: Rene J Buesa To: histonet@lists.utsouthwestern.edu ; 'alan taylor' ; kemlo Sent: Saturday, April 04, 2009 8:01 PM Subject: RE: [Histonet] Nice one Kemlo OK! Ren? J. --- On Sat, 4/4/09, kemlo wrote: From: kemlo Subject: RE: [Histonet] Nice one Kemlo To: rjbuesa@yahoo.com, histonet@lists.utsouthwestern.edu, "'alan taylor'" Date: Saturday, April 4, 2009, 2:28 PM I apologise over this error over your gender; maybe it's because you are so caring and the stereotype takes over. But I think anyone that takes you on must go through me, ok? Your student 38 years in Pathology and still learning sometimes from you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 04 April 2009 18:02 To: histonet@lists.utsouthwestern.edu; alan taylor Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am "a legitimate April Fool" and I intend to keep doing what I am doing for as long as I can. Ren? J. --- On Sat, 4/4/09, alan taylor wrote: From: alan taylor Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Sun Apr 5 02:48:55 2009 From: kemlo <@t> f2s.com (kemlo) Date: Sun Apr 5 02:49:13 2009 Subject: [Histonet] Problems with mouse brains In-Reply-To: References: <040346FA7309BD439C327F97D4C4D69B04A1C481@ISIS.fhcrc.org> Message-ID: Agree, poor formalin fixation will allow nuclear bubbling which some authorities put down to the effects of heat on the sub optimally stabilised proteins. What a marvellous phrase "your formol is not well tamponated". Wish I had thought of that...... I will of course steal it if you allow such plagiarism? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ana Resendes Sent: 04 April 2009 15:16 To: Randolph-Habecker, Julie Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Problems with mouse brains have very light H&E staining: first you should check if either your eosin or haematoxilin are over used nuclear bubbling: can be a lot of things but can be inclusion. Brain sections are big so make at least a 24 h inclusion process. and extensive tissue cracking: could be over heating or bad inclusion. You have to check if reagents in inclusors are ok or over passed, I would change all reagents. I also have noticed some formalin pigment: your formol is not well tamponated 2009/4/3 Randolph-Habecker, Julie > Folks, > > I need some input on a problem we're seeing with some mouse brain > samples. The samples are from new born mice P0 to P14 which have been > fixed in 10% NBF (Fisher brand and well within expiration date) for 72 > hours. They are then transferred to 70% etoh and processed on an 8 hour > process. After the tissue is embedded, we are taking sagital sections, > drying them overnight, and then baking overnight at 60C. They are then > stained with H&E. This has been working great but now all of a sudden we > have very light H&E staining, nuclear bubbling, and extensive tissue > cracking. I also have noticed some formalin pigment. > > My first thought is that there was difference in formalin or time in > formalin but that is not the case. I also wondered if the tissue might > be exposed to excessive heat. We checked all of the temperatures in our > processor, embedding center, and water baths - all were within > tolerance. > > Any ideas what might cause all three artifacts? Could it be from > inadequate paraffin infiltration? > > Any help would be greatly appreciated! > > Thanks, > > Julie > > Julie Randolph-Habecker, Ph.D. > Staff Scientist - Director > Experimental Histopathology Shared Resource > Fred Hutchinson Cancer Research Center > 1100 Fairview Ave, N. DE-360 (Please note new location) > Seattle WA 98109-1024 > 206-667-6119 > jhabecke@fhcrc.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Ana Resendes DVM, MSc, PhD Veterinary pathologist Centro de Investiga??o de Recursos Naturais (CIRN) Sec??o de Anatomia e Taxonomia Zool?gicas, Departamento de Biologia, Universidade dos A?ores. Rua da M?e de Deus, 58 - Apartado 1422 P - 9501-801 Ponta Delgada (A?ores) Portugal Tel. (+351) 296 650 000 ext. 1109 Fax (+351) 296 650 100 http://www.uac.pt/~pherg/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Sun Apr 5 02:52:52 2009 From: kemlo <@t> f2s.com (kemlo) Date: Sun Apr 5 02:53:04 2009 Subject: [Histonet] RE: Histonet Digest, Vol 65, Issue 7 In-Reply-To: References: <4ad895dd0002c2fa@HolyRedeemer.com> Message-ID: <24AE66C82883453D8E8D2472400C9B88@KemloPC> As a recovering Cytologist in the UK I think we have to screen about 7,000 slides per annum, but a 'Checker' less than that and a Medic even less. But the rules may have changed as I've been out of Cytology for a number of years. I know that Jade will have increased the number of women wanting smears (can we can LBC smears?). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: 03 April 2009 13:42 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 65, Issue 7 Hello Histonetters, I understand most of the professionals on this website are histology professionals, however, I thought I would give it a try since I have not found a cytology listserv yet. Does anyone know how many slides per day a cytotechnologist would screen within a private lab setting (On average)? Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 Hi Alyssa - Get ready...here is the dirt CAP follows CLIA-88, and both are quite clear on limits for Cytotech screening. They are "no more than 100 slides in 24 hours" and include gyn and non-gyn including "new routine slides, 10% rescreen slides, and 5 year look back negative slides" "the maximum workload of 100 slides can be completed in no less than an 8-hour workday. This workload can also be expressed as slides per hour and is 12.5 slides/hour. These total limits apply regardless of the number of laboratories in which an individual works on a given day." Wait! it gets better.... "For primary screening of gyn liquid based preps" (read as Thin Prep) "each slide must be counted as a single slide for the purpose of workload recording." "For primary screening of non-gyn liquid based preps" (read as Thin Prep) "each slide may be counted as one-half slide for the purpose of workload recording, provided that the cells are dispersed over one-half or less of the total available slide area." Wait! there's still more... "Workload calculations may vary with the use of automated screening instruments. Laboratories should follow manufacturers instructions for workload calculations and must assure that CLIAA-88 requirements are fulfilled." and just when you thought it was safe.... "The laboratory director must establish the maximum workload (based on the capability/documented performance evaluation) for each individual examining slides and the limit must be reassessed at least every 6 months." The regulations go on to explain how the reassessment is to be done...blah, blah, blah.... Though my background and education are in Histology, I've served as technical supervisor over cytology departments for over 14 years, and the above listed %#@&! is why Cytology can drive you up the wall. Our cytotech's, when we had them, screened no more than 80 slides/day, when all they did was screen. No pulling slides, no running up to do an FNA....just sitting non-stop and screening slides. UGH! Sorry Histo folk - but some of you are probably in the same position as I am, so please feel free to chime in. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax ---------------------------------------------------------------------------- ----- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From resendes.ana <@t> gmail.com Sun Apr 5 06:10:19 2009 From: resendes.ana <@t> gmail.com (Ana Resendes) Date: Sun Apr 5 06:10:27 2009 Subject: [Histonet] Problems with mouse brains In-Reply-To: References: <040346FA7309BD439C327F97D4C4D69B04A1C481@ISIS.fhcrc.org> Message-ID: Hello Kemlo, I am Portuguese-Spanish.... so we use the word "tamp?n" that I think I miss translated to "tamponated"..really do not know if the word exists! but apparently it does not! :) I want to mean when you add salt to the formol dilution in destilated water in order to prepare your 10% buffered formalin... a believe the word is "buffered" So sorry..for my wrong translation....I was in a hurry...what I want to say is THAT YOUR FORMALIN IN NOT WELL BUFFERED :) thank you! and Yes, you should be carefull your brain is well fixated in formalin at least for 24 hour. 2009/4/5 kemlo > Agree, poor formalin fixation will allow nuclear bubbling which some > authorities put down to the effects of heat on the sub optimally stabilised > proteins. What a marvellous phrase "your formol is not well tamponated". > Wish I had thought of that...... > > I will of course steal it if you allow such plagiarism? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ana > Resendes > Sent: 04 April 2009 15:16 > To: Randolph-Habecker, Julie > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Problems with mouse brains > > have very light H&E staining: first you should check if either your eosin > or > haematoxilin are over used > > > nuclear bubbling: can be a lot of things but can be inclusion. > Brain sections are big so make at least a 24 h inclusion process. > > and extensive tissue cracking: could be over heating or bad inclusion. You > have to check if reagents in inclusors are ok or over passed, I would > change > all reagents. > > I also have noticed some formalin pigment: your formol is not well > tamponated > > > 2009/4/3 Randolph-Habecker, Julie > > > Folks, > > > > I need some input on a problem we're seeing with some mouse brain > > samples. The samples are from new born mice P0 to P14 which have been > > fixed in 10% NBF (Fisher brand and well within expiration date) for 72 > > hours. They are then transferred to 70% etoh and processed on an 8 hour > > process. After the tissue is embedded, we are taking sagital sections, > > drying them overnight, and then baking overnight at 60C. They are then > > stained with H&E. This has been working great but now all of a sudden we > > have very light H&E staining, nuclear bubbling, and extensive tissue > > cracking. I also have noticed some formalin pigment. > > > > My first thought is that there was difference in formalin or time in > > formalin but that is not the case. I also wondered if the tissue might > > be exposed to excessive heat. We checked all of the temperatures in our > > processor, embedding center, and water baths - all were within > > tolerance. > > > > Any ideas what might cause all three artifacts? Could it be from > > inadequate paraffin infiltration? > > > > Any help would be greatly appreciated! > > > > Thanks, > > > > Julie > > > > Julie Randolph-Habecker, Ph.D. > > Staff Scientist - Director > > Experimental Histopathology Shared Resource > > Fred Hutchinson Cancer Research Center > > 1100 Fairview Ave, N. DE-360 (Please note new location) > > Seattle WA 98109-1024 > > 206-667-6119 > > jhabecke@fhcrc.org > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Ana Resendes > DVM, MSc, PhD > Veterinary pathologist > Centro de Investiga??o de Recursos Naturais (CIRN) > Sec??o de Anatomia e Taxonomia Zool?gicas, Departamento de Biologia, > Universidade dos A?ores. > Rua da M?e de Deus, 58 - Apartado 1422 > P - 9501-801 Ponta Delgada (A?ores) > Portugal > Tel. (+351) 296 650 000 ext. 1109 > Fax (+351) 296 650 100 > http://www.uac.pt/~pherg/ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- Ana Resendes DVM, MSc, PhD Veterinary Pathologist Centro de Investiga??o de Recursos Naturais (CIRN) Sec??o de Anatomia e Taxonomia Zool?gicas, Departamento de Biologia, Universidade dos A?ores. Rua da M?e de Deus, 58 - Apartado 1422 P - 9501-801 Ponta Delgada (A?ores) Portugal Tel. (+351) 296 650 000 ext. 1109 Fax (+351) 296 650 100 http://www.uac.pt/~pherg/ From arvidsonkristen <@t> yahoo.com Sun Apr 5 10:14:36 2009 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Sun Apr 5 10:14:39 2009 Subject: [Histonet] IHC Stainers Message-ID: <641180.86868.qm@web65702.mail.ac4.yahoo.com> Hello- I am looking for any comments (positive or negative) on IHC stainers.? Any info on DAKO, Ventana, Leica, and Biocare would be great.? Service, quality,?ease of use, etc.? I have very little knowledge of any of these as I have not done IHC for many years.? Thanks for all your input!! -Kristen From rjbuesa <@t> yahoo.com Sun Apr 5 10:28:05 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Apr 5 10:28:09 2009 Subject: [Histonet] IHC Stainers In-Reply-To: <641180.86868.qm@web65702.mail.ac4.yahoo.com> Message-ID: <951563.53738.qm@web65703.mail.ac4.yahoo.com> Very recently (just a few days ago)?this topic was discussed in HistoNet. You would be better?served if you check the Archives for answers. Ren? J. --- On Sun, 4/5/09, kristen arvidson wrote: From: kristen arvidson Subject: [Histonet] IHC Stainers To: "histonet" Date: Sunday, April 5, 2009, 11:14 AM Hello- I am looking for any comments (positive or negative) on IHC stainers.? Any info on DAKO, Ventana, Leica, and Biocare would be great.? Service, quality,?ease of use, etc.? I have very little knowledge of any of these as I have not done IHC for many years.? Thanks for all your input!! -Kristen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From drvet_anjan <@t> hotmail.com Sun Apr 5 11:41:59 2009 From: drvet_anjan <@t> hotmail.com (anjan kumar) Date: Sun Apr 5 11:42:02 2009 Subject: [Histonet] silanising slides Message-ID: hi everyone, can any one tell me how many slides can be processed with 10ml of 3-aminopropyltriethoxysilane. i have a 100ml solution from Sigma. Regards, Dr. Anjan Kumar.K.R M.V.Sc Scholar Dept. of Veterinary Pathology Madras Veterinary College Chennai-7 India email: drvet_anjan@hotmail.com Phone: +91-9940475801 _________________________________________________________________ Windows Live Messenger. Multitasking at its finest. http://www.microsoft.com/india/windows/windowslive/messenger.aspx From rsrichmond <@t> gmail.com Sun Apr 5 12:33:34 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sun Apr 5 12:33:40 2009 Subject: [Histonet] nice one Kemlo Message-ID: The well known German poet Rainer Maria Rilke (1875-1926) was originally named Ren?, and Germanized the name to Rainer when he was a young man. His mother supposedly gave him the name in memory of a recently deceased older sister, but the story that she dressed the future poet as a girl may be untrue. Bernhard Blume, my major professor in 1959 and an elderly man then (I majored in German in college, and wrote my senior thesis on Rilke, back before he got famous in English) told me that his baby pictures showed him dressed quite like the young Rilke. Bob Richmond Knoxville TN From rjbuesa <@t> yahoo.com Sun Apr 5 12:51:43 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Apr 5 12:51:47 2009 Subject: [Histonet] silanising slides In-Reply-To: Message-ID: <523442.29300.qm@web65710.mail.ac4.yahoo.com> Until your solution is used up. The solution is homogeneous and immersing a slide inside it will just take the corresponding amount of silane. Different thing with staining solutions that are usually weak and last for a limited number of slides. Ren? J. --- On Sun, 4/5/09, anjan kumar wrote: From: anjan kumar Subject: [Histonet] silanising slides To: "triple immunohistochem" Date: Sunday, April 5, 2009, 12:41 PM hi everyone, can any one tell me how many slides can be processed with 10ml of 3-aminopropyltriethoxysilane. i have a 100ml solution from Sigma. Regards, Dr. Anjan Kumar.K.R M.V.Sc Scholar Dept. of Veterinary Pathology Madras Veterinary College Chennai-7 India email: drvet_anjan@hotmail.com Phone: +91-9940475801 _________________________________________________________________ Windows Live Messenger. Multitasking at its finest. http://www.microsoft.com/india/windows/windowslive/messenger.aspx_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Apr 5 12:57:30 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Apr 5 12:57:36 2009 Subject: [Histonet] silanising slides In-Reply-To: <523442.29300.qm@web65710.mail.ac4.yahoo.com> References: <523442.29300.qm@web65710.mail.ac4.yahoo.com> Message-ID: <85687299AA1A453F86838B679B3C922C@ihctechq9h2qof> Is your solution supposed to be diluted or used neat? I agree with Rene, the solution will coat the slide and can be used until it is used until it is gone, it does not go bad. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pruegg@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, April 05, 2009 11:52 AM To: triple immunohistochem; anjan kumar Subject: Re: [Histonet] silanising slides Until your solution is used up. The solution is homogeneous and immersing a slide inside it will just take the corresponding amount of silane. Different thing with staining solutions that are usually weak and last for a limited number of slides. Ren? J. --- On Sun, 4/5/09, anjan kumar wrote: From: anjan kumar Subject: [Histonet] silanising slides To: "triple immunohistochem" Date: Sunday, April 5, 2009, 12:41 PM hi everyone, can any one tell me how many slides can be processed with 10ml of 3-aminopropyltriethoxysilane. i have a 100ml solution from Sigma. Regards, Dr. Anjan Kumar.K.R M.V.Sc Scholar Dept. of Veterinary Pathology Madras Veterinary College Chennai-7 India email: drvet_anjan@hotmail.com Phone: +91-9940475801 _________________________________________________________________ Windows Live Messenger. Multitasking at its finest. http://www.microsoft.com/india/windows/windowslive/messenger.aspx___________ ____________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sharon.osborn <@t> comcast.net Sun Apr 5 13:54:36 2009 From: sharon.osborn <@t> comcast.net (Sharon Osborn) Date: Sun Apr 5 13:54:40 2009 Subject: [Histonet] cytology screening requirements Message-ID: <000c01c9b61f$f7e8f290$e7bad7b0$@osborn@comcast.net> Terri and Allysa, Terri, just reading what you have written about CLIA '88 regs for Cytology screening reminds me why I no longer do cytology and just do histology. I did both for over 15 years, leaving several years prior to CLIA 88. I worked in rural area designated as a regional medical center. We had two pathologists, 2 histotechs, 1 cyto tech(me) plus I was backup histo. I did ALL the cytology prep work, staining, cover slipping, screening, filing and ordering. The secretary did the recording and the billing. My work was very interesting for I saw it all in fluids and Paps. We had some very progressive specialist clinicians so that when needle aspirates first came out, we were doing them. My pathologists' attitude was that any meeting/training was worth it if we learned one thing well and implemented it. They would challenge me and I would challenge them on new technics, ideas and best health care practice. Some days a person can screen more slides while other days screen less. This can be due to the type/quality of the slide, or a number of different variables. This was the days before Thin Prep when the spatula was used to scrap the cervix. The quality of the slides depended upon the skill of the clinician or whomever was doing the scraping. My pathologists did a 10% rescreen of my work before it was a requirement because we felt that was the correct process for continuing evaluation. Sharon Osborn IHC-QC ThermoFisherScientific Fremont, CA From kdwyer3322 <@t> aol.com Sun Apr 5 14:19:11 2009 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sun Apr 5 14:19:26 2009 Subject: [Histonet] Texas Society for Histotechnology 2009 Symposium/Convention-Registration is Open Message-ID: <8CB843A88C97526-258-497A@webmail-mh14.sysops.aol.com> Dear Histonetters: It's not too late to sign up for the Texas Society for Histotechnology meeting.? Pre registration for the meeting is still available and deadline is MAY 25, 2009. Attached is the 2009 TSH convention?preview?in Austin,Texas May 29-31, 2009 (not Memorial Day weekend).? The Convention will be held at the Hyatt Regency, 208 Barton Springs Road, Austin,Texas.? Please join us for a great "Texas Style" meeting.? All workshops will be contact hour approved by NSH. For more information or a e-mail copy of the program contact: Veronida@baylorhealth.edu or kdywer3322@aol.com or www.txsh.org Friday, May 29, 2009 WS#1- Skip Brown-Basics Dynamics of Fixation and Processing WS#2 ? Damien Matusiak- Digital Evolution: Taking the Pathologists from the Basement to the Bedside Saturday, May 30, 2009 WS#3 - Jan Gardner - Competency Assessment Program WS#4 ? Jennifer Hofecker ? Understanding CJD WS#5 ? Nora Lacey ? 2009 Latest Antibody Information and IHC Applications WS#6 ? David Tate ? Serial Killers WS#7 ? Skip Brown ? The Wax Museum: Understanding Paraffins WS #8 ? Bill DeSalvo/Lamar Jones ? From Concept to Reality-Implementing Continuous Process Flow in the Histology Lab WS#9 ? Donna Willis ? Rapid Tissue Processing, A New Era 2009 WS#10 ? Jim Chalmers ? Pretreatments ? An Important Toll in Immunohistochemistry WS #11 ? Terri Crook, MD ? Cytology for th e Histotechnologist WS#12 ? Charlotte Sanders ? Safety Can Be Fun!! Sunday, May 31, 2009 WS #13 ? Jennifer Hofecker ? The Wonderful World of Neuropathology WS #14 ? Bonnie Whitaker/Pam Marcum ? Two Crazy Cat Ladies Give Their Opinions of Life, Liberty and Lab Management WS#15 ? Debbie Siena/Brent Hart ? Immunohistochemistry: Validation vs. Optimization WS#16 ? Debra Cohen/Sharon Tandy?? Paraffin-Embedded Tissue Fluorescence In Situ Hybridization (PET FISH) ? From kdwyer3322 <@t> aol.com Sun Apr 5 14:19:11 2009 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sun Apr 5 14:19:28 2009 Subject: [Histonet] Texas Society for Histotechnology 2009 Symposium/Convention-Registration is Open Message-ID: <8CB843A88C97526-258-497A@webmail-mh14.sysops.aol.com> Dear Histonetters: It's not too late to sign up for the Texas Society for Histotechnology meeting.? Pre registration for the meeting is still available and deadline is MAY 25, 2009. Attached is the 2009 TSH convention?preview?in Austin,Texas May 29-31, 2009 (not Memorial Day weekend).? The Convention will be held at the Hyatt Regency, 208 Barton Springs Road, Austin,Texas.? Please join us for a great "Texas Style" meeting.? All workshops will be contact hour approved by NSH. For more information or a e-mail copy of the program contact: Veronida@baylorhealth.edu or kdywer3322@aol.com or www.txsh.org Friday, May 29, 2009 WS#1- Skip Brown-Basics Dynamics of Fixation and Processing WS#2 ? Damien Matusiak- Digital Evolution: Taking the Pathologists from the Basement to the Bedside Saturday, May 30, 2009 WS#3 - Jan Gardner - Competency Assessment Program WS#4 ? Jennifer Hofecker ? Understanding CJD WS#5 ? Nora Lacey ? 2009 Latest Antibody Information and IHC Applications WS#6 ? David Tate ? Serial Killers WS#7 ? Skip Brown ? The Wax Museum: Understanding Paraffins WS #8 ? Bill DeSalvo/Lamar Jones ? From Concept to Reality-Implementing Continuous Process Flow in the Histology Lab WS#9 ? Donna Willis ? Rapid Tissue Processing, A New Era 2009 WS#10 ? Jim Chalmers ? Pretreatments ? An Important Toll in Immunohistochemistry WS #11 ? Terri Crook, MD ? Cytology for th e Histotechnologist WS#12 ? Charlotte Sanders ? Safety Can Be Fun!! Sunday, May 31, 2009 WS #13 ? Jennifer Hofecker ? The Wonderful World of Neuropathology WS #14 ? Bonnie Whitaker/Pam Marcum ? Two Crazy Cat Ladies Give Their Opinions of Life, Liberty and Lab Management WS#15 ? Debbie Siena/Brent Hart ? Immunohistochemistry: Validation vs. Optimization WS#16 ? Debra Cohen/Sharon Tandy?? Paraffin-Embedded Tissue Fluorescence In Situ Hybridization (PET FISH) ? From Jackie.O'Connor <@t> abbott.com Sun Apr 5 20:25:30 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Sun Apr 5 20:25:52 2009 Subject: [Histonet] nice one Kemlo In-Reply-To: Message-ID: My grandfather from Slovakia was Jan. My name is Jackie, I have five sisters, two are Jeri and Stevie. My parents had a son after they had five daughters and named him Ernie. Sometimes people just run out of good names for their kids. I happen to like Rene'. It beats Apple or Moon Unit Zappa. From AnthonyH <@t> chw.edu.au Sun Apr 5 23:21:14 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Apr 5 23:21:32 2009 Subject: [Histonet] Burned Tissue In-Reply-To: <8CB81D89985EA77-1380-1BCB@webmail-md08.sysops.aol.com> Message-ID: I would suggest checking the fixation. We had similar problems if the tissue was not completely fixed. Are the tissues free to move around in the cassettes (ie have free unhindered access to the fixative)? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com Sent: Friday, 3 April 2009 5:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Burned Tissue Recently we are experiencing random blocks of tissue (approx. 3 blocks out of 400/2x) that appear to be "burned".? The tissue is prostate and the staining looks dark.? Upon recutting, sometimes it is better, sometimes it is not, which negates my ability to rule out the stainer completely. We process on a 2 hour schedule using the Peloris and all tissue is processed together.? I am not understanding how, out of a 12 part prostate, only one block stains so dark so randomly.? When recut, some blocks are better, some still have the dark staining. Does anyone have any suggestions? Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From bernietaupin <@t> ymail.com Sun Apr 5 23:25:20 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Sun Apr 5 23:25:25 2009 Subject: [Histonet] Entamoeba Trophozoites In-Reply-To: <9ED35C9D2C5B4AFB834241AC979BDEA0@KemloPC> References: <635986.93921.qm@web65713.mail.ac4.yahoo.com> <511036.54656.qm@web43504.mail.sp1.yahoo.com> <9ED35C9D2C5B4AFB834241AC979BDEA0@KemloPC> Message-ID: <646850.95235.qm@web43508.mail.sp1.yahoo.com> It's amazing how such a simple observation can bring out the worst (and most subjective rants) in some people, eh? How many personal insults and names did you just hurl at me here, "Sonny"? the most ironic part is that people like you jump down my throat for my comments but don't seem to be bright enough to restrain themselves from making the exact same sort of comments. And YOU're the one calling ME a "half wit"! ________________________________ From: kemlo To: Bernie Taupin ; histonet@lists.utsouthwestern.edu Sent: Saturday, April 4, 2009 9:15:02 AM Subject: RE: [Histonet] Entamoeba Trophozoites Is there a problem with that sonny? Maybe you ought to get the gender right before you attack someone who tries very hard to help people. Ignorant people, such as you, usually attack those brighter than themselves. The Middle Ages beckons you, sonny! Kemlo Rogerson Pathology Manager used to dealing with half wits (comes with the territory). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: 04 April 2009 06:21 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Entamoeba Trophozoites Anyone else think this Rene fella comes across as a real know-it-all? ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Lynette Pavelich Sent: Friday, April 3, 2009 4:12:15 PM Subject: [Histonet] Entamoeba Trophozoites Lynette: Since it was received in saline and the tissue has its normal consistency I would prepare a squash (squash the tissue between 2 glass slides not between coverslips) with a rotary movement so you have 2 large areas of squashed tissue. After that, air dry, fix with methanol, air dry again and stain with Giemsa. That is what I would do. Try it if you do not have a better option. Ren? J. --- On Fri, 4/3/09, Lynette Pavelich wrote: From: Lynette Pavelich Subject: [Histonet] Entamoeba Trophozoites To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Friday, April 3, 2009, 3:09 PM I need help! Just received a needle biopsy of liver submitted in normal saline. They did all the routine cultures/ fungal, and are requesting a wet mount on this tissue for Entamoeba Trophozoites. I have contacted all of my resources in and out of state, and they cannot help me. HELP!! thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Sun Apr 5 23:27:47 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Sun Apr 5 23:27:51 2009 Subject: [Histonet] Nice one Kemlo In-Reply-To: <658345.87226.qm@web65701.mail.ac4.yahoo.com> References: <658345.87226.qm@web65701.mail.ac4.yahoo.com> Message-ID: <648478.7269.qm@web43507.mail.sp1.yahoo.com> 1. How is anyone supposed to know your gender online when you have such an androgynous name? 2. what does your gender have to do with this in the first place? gender equality is gender equality. but, not surprisingly, none of the feminists on this list have come out to say that. though i bet some would if i said something sexist, eh? ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; alan taylor Sent: Saturday, April 4, 2009 1:01:36 PM Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am "a legitimate April Fool" and I intend to keep doing what I am doing for as long as I can. Ren? J. --- On Sat, 4/4/09, alan taylor wrote: From: alan taylor Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Sun Apr 5 23:34:12 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Sun Apr 5 23:34:16 2009 Subject: [Histonet] Entamoeba Trophozoites In-Reply-To: <9ED35C9D2C5B4AFB834241AC979BDEA0@KemloPC> References: <635986.93921.qm@web65713.mail.ac4.yahoo.com> <511036.54656.qm@web43504.mail.sp1.yahoo.com> <9ED35C9D2C5B4AFB834241AC979BDEA0@KemloPC> Message-ID: <200075.62640.qm@web43515.mail.sp1.yahoo.com> > I am a HE and not a SHE Oh, HAHAHA! Nice one, Kemlo! Disregard my last noes re: Gender and maybe, I dunno, CHECK YOUR OWN FACTS BEFORE CHEWING SOMEONE OUT FOR THEIRS? Everyone on this list is so quick to jump down someone else's throat for "telling it like it is", completely ignorant of their own breaches in tact, their own ignorances, their own off-topicness. Everyone loves pointing the finger, yet no one can tolerate it when its actually them at fault. You guys crack me up with your logic sometimes. I fear for the world that the safety of so many imporant biopsies are trusted to so many of yours' easily-agitated, ignorant hands! ________________________________ From: kemlo To: Bernie Taupin ; histonet@lists.utsouthwestern.edu Sent: Saturday, April 4, 2009 9:15:02 AM Subject: RE: [Histonet] Entamoeba Trophozoites Is there a problem with that sonny? Maybe you ought to get the gender right before you attack someone who tries very hard to help people. Ignorant people, such as you, usually attack those brighter than themselves. The Middle Ages beckons you, sonny! Kemlo Rogerson Pathology Manager used to dealing with half wits (comes with the territory). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: 04 April 2009 06:21 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Entamoeba Trophozoites Anyone else think this Rene fella comes across as a real know-it-all? ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Lynette Pavelich Sent: Friday, April 3, 2009 4:12:15 PM Subject: [Histonet] Entamoeba Trophozoites Lynette: Since it was received in saline and the tissue has its normal consistency I would prepare a squash (squash the tissue between 2 glass slides not between coverslips) with a rotary movement so you have 2 large areas of squashed tissue. After that, air dry, fix with methanol, air dry again and stain with Giemsa. That is what I would do. Try it if you do not have a better option. Ren? J. --- On Fri, 4/3/09, Lynette Pavelich wrote: From: Lynette Pavelich Subject: [Histonet] Entamoeba Trophozoites To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Friday, April 3, 2009, 3:09 PM I need help! Just received a needle biopsy of liver submitted in normal saline. They did all the routine cultures/ fungal, and are requesting a wet mount on this tissue for Entamoeba Trophozoites. I have contacted all of my resources in and out of state, and they cannot help me. HELP!! thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lactose.intolerant <@t> yahoo.com Sun Apr 5 23:46:28 2009 From: lactose.intolerant <@t> yahoo.com (aa aa) Date: Sun Apr 5 23:46:33 2009 Subject: [Histonet] Entamoeba Trophozoites In-Reply-To: <9ED35C9D2C5B4AFB834241AC979BDEA0@KemloPC> Message-ID: <599459.65491.qm@web55303.mail.re4.yahoo.com> All critique and contention with Bernie's opinions aside, I have recently grown flabbergasted by the surprising unprofessionalism of some of HistoNet's responders, an the complete, utter and profound lack of tact expressed in some of the Reply-to-All messages. I mean, honestly. We have hotheaded individuals like Kemlo mocking and "correcting" people about things that they were correct about to begin with, we have individuals getting laughably snagged by the snares of irresistable, pointless argumentativism and we simply have indivudals proving, over and over again, that the worsat of human nature tends to come out when replying to internet Trolls. So do us all a favor, Bernie, Kemlo, Rene and everyone else here and give it a freakign rest already. If you're engaging trolls in arguments or seemingly-witty (yet in hindsight, ignorantly and laughably incorrect) commentary, then you're as much of a problem as the Troll himself, and I should hope to see such inane individuals (as well as all the collective eye-rolling and grimacing that they cause) exilded from this list!!!!!!! ~L. --- On Sat, 4/4/09, kemlo wrote: From: kemlo Subject: RE: [Histonet] Entamoeba Trophozoites To: "'Bernie Taupin'" , histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 9:15 AM Is there a problem with that sonny? Maybe you ought to get the gender right before you attack someone who tries very hard to help people. Ignorant people, such as you, usually attack those brighter than themselves. The Middle Ages beckons you, sonny! Kemlo Rogerson Pathology Manager used to dealing with half wits (comes with the territory). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: 04 April 2009 06:21 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Entamoeba Trophozoites Anyone else think this Rene fella comes across as a real know-it-all? ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Lynette Pavelich Sent: Friday, April 3, 2009 4:12:15 PM Subject: [Histonet] Entamoeba Trophozoites Lynette: Since it was received in saline and the tissue has its normal consistency I would prepare a squash (squash the tissue between 2 glass slides not between coverslips) with a rotary movement so you have 2 large areas of squashed tissue. After that, air dry, fix with methanol, air dry again and stain with Giemsa. That is what I would do. Try it if you do not have a better option. Ren? J. --- On Fri, 4/3/09, Lynette Pavelich wrote: From: Lynette Pavelich Subject: [Histonet] Entamoeba Trophozoites To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Friday, April 3, 2009, 3:09 PM I need help! Just received a needle biopsy of liver submitted in normal saline. They did all the routine cultures/ fungal, and are requesting a wet mount on this tissue for Entamoeba Trophozoites. I have contacted all of my resources in and out of state, and they cannot help me. HELP!! thanks, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lactose.intolerant <@t> yahoo.com Sun Apr 5 23:55:27 2009 From: lactose.intolerant <@t> yahoo.com (aa aa) Date: Sun Apr 5 23:55:31 2009 Subject: [Histonet] Histology "Expert" Fees/Salaries Message-ID: <915575.95961.qm@web55306.mail.re4.yahoo.com> Did anyone else happen to see that online clip from "Dateline" regarding "Histology "Expert" Fees/Salaries"? I am curious to know exactly what they mean by "expert"...? See clip here: http://tinyurl.com/2g9mqh From llewllew <@t> shaw.ca Mon Apr 6 00:14:25 2009 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Mon Apr 6 00:15:10 2009 Subject: [Histonet] =?iso-8859-1?q?Ren=E9_as_a_name=2E?= In-Reply-To: <648478.7269.qm@web43507.mail.sp1.yahoo.com> References: <658345.87226.qm@web65701.mail.ac4.yahoo.com> <648478.7269.qm@web43507.mail.sp1.yahoo.com> Message-ID: For all those not cognizant of it, including the eminently intellectual Mr. Taupin, the name "Ren?" is a male French name. The tip off is the acute accented "?" at the end. The female name is "Ren?e". Ren?'s name is not androgynous at all, it is quite clearly male, and anyone who has studied French should be aware of it. Mr. Taupin is clearly trolling, so could we please have him removed from this group? Bryan Llewellyn ----- Original Message ----- From: "Bernie Taupin" To: ; ; "alan taylor" Sent: Sunday, April 05, 2009 9:27 PM Subject: Re: [Histonet] Nice one Kemlo 1. How is anyone supposed to know your gender online when you have such an androgynous name? 2. what does your gender have to do with this in the first place? gender equality is gender equality. but, not surprisingly, none of the feminists on this list have come out to say that. though i bet some would if i said something sexist, eh? ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; alan taylor Sent: Saturday, April 4, 2009 1:01:36 PM Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am "a legitimate April Fool" and I intend to keep doing what I am doing for as long as I can. Ren? J. --- On Sat, 4/4/09, alan taylor wrote: From: alan taylor Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Mon Apr 6 01:24:31 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Mon Apr 6 01:24:36 2009 Subject: =?iso-8859-1?Q?Re=3A_=5BHistonet=5D_Ren=E9_as_a_name=2E?= In-Reply-To: References: <658345.87226.qm@web65701.mail.ac4.yahoo.com> <648478.7269.qm@web43507.mail.sp1.yahoo.com> Message-ID: <996419.93479.qm@web43515.mail.sp1.yahoo.com> > For all those not cognizant of it, including the eminently intellectual Mr. Taupin Dear Doug Llewellyn, Please note that I am the one who originally referred to Rene as "he". It is this Kemlo person you should be lecturing, not me. If you see me as being someone "not cognizant of it", then you need to get your money back from whomever taught you such lackluster reading comprehension. And, do please refer back to my earlier email about list-members getting all hot-headed and flying off the handle with incorrect and quote offensively presumptuous replies before thinking them out logically. ________________________________ From: Bryan Llewellyn To: Histonet Sent: Monday, April 6, 2009 1:14:25 AM Subject: [Histonet] Ren? as a name. For all those not cognizant of it, including the eminently intellectual Mr. Taupin, the name "Ren?" is a male French name. The tip off is the acute accented "?" at the end. The female name is "Ren?e". Ren?'s name is not androgynous at all, it is quite clearly male, and anyone who has studied French should be aware of it. Mr. Taupin is clearly trolling, so could we please have him removed from this group? Bryan Llewellyn ----- Original Message ----- From: "Bernie Taupin" To: ; ; "alan taylor" Sent: Sunday, April 05, 2009 9:27 PM Subject: Re: [Histonet] Nice one Kemlo 1. How is anyone supposed to know your gender online when you have such an androgynous name? 2. what does your gender have to do with this in the first place? gender equality is gender equality. but, not surprisingly, none of the feminists on this list have come out to say that. though i bet some would if i said something sexist, eh? ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; alan taylor Sent: Saturday, April 4, 2009 1:01:36 PM Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am "a legitimate April Fool" and I intend to keep doing what I am doing for as long as I can. Ren? J. --- On Sat, 4/4/09, alan taylor wrote: From: alan taylor Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lactose.intolerant <@t> yahoo.com Mon Apr 6 01:27:38 2009 From: lactose.intolerant <@t> yahoo.com (aa aa) Date: Mon Apr 6 01:27:42 2009 Subject: =?iso-8859-1?Q?Re=3A_=5BHistonet=5D_Ren=E9_as_a_name=2E?= In-Reply-To: Message-ID: <862033.32888.qm@web55306.mail.re4.yahoo.com> My goodness, I am afraid to admit that flippant remarks like this from the list are making me think that perhaps Mr. Taupin might have a point after all.... --- On Mon, 4/6/09, Bryan Llewellyn wrote: From: Bryan Llewellyn Subject: [Histonet] Ren? as a name. To: "Histonet" Date: Monday, April 6, 2009, 1:14 AM For all those not cognizant of it, including the eminently intellectual Mr. Taupin, the name "Ren?" is a male French name. The tip off is the acute accented "?" at the end. The female name is "Ren?e". Ren?'s name is not androgynous at all, it is quite clearly male, and anyone who has studied French should be aware of it. Mr. Taupin is clearly trolling, so could we please have him removed from this group? Bryan Llewellyn ----- Original Message ----- From: "Bernie Taupin" To: ; ; "alan taylor" Sent: Sunday, April 05, 2009 9:27 PM Subject: Re: [Histonet] Nice one Kemlo 1. How is anyone supposed to know your gender online when you have such an androgynous name? 2. what does your gender have to do with this in the first place? gender equality is gender equality. but, not surprisingly, none of the feminists on this list have come out to say that. though i bet some would if i said something sexist, eh? ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; alan taylor Sent: Saturday, April 4, 2009 1:01:36 PM Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am "a legitimate April Fool" and I intend to keep doing what I am doing for as long as I can. Ren? J. --- On Sat, 4/4/09, alan taylor wrote: From: alan taylor Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Mon Apr 6 01:52:11 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Mon Apr 6 01:52:14 2009 Subject: [Histonet] onalighternote In-Reply-To: <7722595275A4DD4FA225B92CDBF174A17457783C75@EXC-MBX3.cfs.le.ac.uk> References: <930298.3241.qm@web43515.mail.sp1.yahoo.com> <2A582E8156B45F468A62D1F1D20AF083713FA3@EX-BE08.ohsu.edu> <7722595275A4DD4FA225B92CDBF174A17457783C75@EXC-MBX3.cfs.le.ac.uk> Message-ID: <963685.8582.qm@web43504.mail.sp1.yahoo.com> > I have just had eeees from Bernie Taupin and Paul Schofield, is this a record??. I, for one, am sort of bummed that R. E. Edwards never got back to us in regards to exactly what these "eeees" are. Aren't "eeees" some sort of drug , R. E.? Are you on this drug?? ________________________________ From: "Edwards, R.E." To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, March 31, 2009 10:15:51 AM Subject: [Histonet] onalighternote I have just had eeees from Bernie Taupin and Paul Schofield, is this a record??. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer > I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Apr 6 02:14:35 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Apr 6 02:14:39 2009 Subject: [Histonet] Nice one Kemlo Message-ID: <86ADE4EB583CE64799A9924684A0FBBF06833D06@wahtntex2.waht.swest.nhs.uk> I think the benefit of getting older SHOULD be that one gets wiser. To get old without acquiring wisdom is a waste. One of those wisdoms is to know when a conversation has ended......... or when there's no point due to the immaturity or lack of insight of some of the audience. You can use that Bernie, to stick at the end of your e-mails as a 'wise saying' (g). Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: 06 April 2009 05:28 To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; alan taylor Subject: Re: [Histonet] Nice one Kemlo 1. How is anyone supposed to know your gender online when you have such an androgynous name? 2. what does your gender have to do with this in the first place? gender equality is gender equality. but, not surprisingly, none of the feminists on this list have come out to say that. though i bet some would if i said something sexist, eh? ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; alan taylor Sent: Saturday, April 4, 2009 1:01:36 PM Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am "a legitimate April Fool" and I intend to keep doing what I am doing for as long as I can. Ren? J. --- On Sat, 4/4/09, alan taylor wrote: From: alan taylor Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Mon Apr 6 02:20:18 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Mon Apr 6 02:20:24 2009 Subject: [Histonet] Nice one Kemlo In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF06833D06@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF06833D06@wahtntex2.waht.swest.nhs.uk> Message-ID: <695693.42984.qm@web43505.mail.sp1.yahoo.com> Kemlo, So do you feel like taking a moment to address why you called me out for something even when I was correct to begin with? ________________________________ From: Kemlo Rogerson To: Bernie Taupin ; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; alan taylor Sent: Monday, April 6, 2009 3:14:35 AM Subject: RE: [Histonet] Nice one Kemlo I think the benefit of getting older SHOULD be that one gets wiser. To get old without acquiring wisdom is a waste. One of those wisdoms is to know when a conversation has ended......... or when there's no point due to the immaturity or lack of insight of some of the audience. You can use that Bernie, to stick at the end of your e-mails as a 'wise saying' (g). Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: 06 April 2009 05:28 To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; alan taylor Subject: Re: [Histonet] Nice one Kemlo 1. How is anyone supposed to know your gender online when you have such an androgynous name? 2. what does your gender have to do with this in the first place? gender equality is gender equality. but, not surprisingly, none of the feminists on this list have come out to say that. though i bet some would if i said something sexist, eh? ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; alan taylor Sent: Saturday, April 4, 2009 1:01:36 PM Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am "a legitimate April Fool" and I intend to keep doing what I am doing for as long as I can. Ren? J. --- On Sat, 4/4/09, alan taylor wrote: From: alan taylor Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Mon Apr 6 03:02:59 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Apr 6 03:03:07 2009 Subject: [Histonet] k-ras test Message-ID: <4063AAB542EE43A0BA6346EA2CE9F0D5@dielangs.at> Dear listmembers, Our histolab wants to go into k-ras testing. There's a commercial product from Chipron LCD array-kit. Has anyone experience with this product? Would anyone recommend it? Thanks in advance Gudrun Lang From lcover <@t> wmhs.com Mon Apr 6 04:42:00 2009 From: lcover <@t> wmhs.com (Cover, Lois) Date: Mon Apr 6 04:42:51 2009 Subject: [Histonet] (no subject) Message-ID: <24F24A72BE11F2409C0613BAD15A496B45823B@MAILC.wmhs.com> I HAVE SUBSCRIBED TO THE HISTONET DIGEST TO GAIN VALUABLE INFORMATION FROM MY PEERS. I THINK IT WOULD BE BEST IF THOSE WHO HAVE ISSUES WITH EACH OTHER "TALK" OFF-LINE. I DON'T WANT TO SPEND VALUABLE TIME READING THIS ENDLESS NONSENSE! Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From resendes.ana <@t> gmail.com Mon Apr 6 05:06:39 2009 From: resendes.ana <@t> gmail.com (Ana Resendes) Date: Mon Apr 6 05:06:45 2009 Subject: [Histonet] Histonet list Message-ID: Would like to thank all histonet users for the important and valuable contents that are discussed in this list. The truth is that as a pathologist I am finally understanding issues regarding histotechnology I did not understand and specially learning by the doubts and discussion people put in this list. I think this is a very valuable tool, so it has to be used with consciousness and reasonableness so it does not lose value and credibility and continue to be used and signed by the greater number of people. Of course for that to happen, people have to be consciousness that time is valuable, since we all work with short time, we all have to deal with daily huge amount of responsabilities, and that contents in the list should always focus the interests of histotechnologist and other interested users....otherwise this fantastic tool that is histonet will fall in disuse and its credibility and use decrease if badly used, what I believe would be loss of a resource for everybody. Thank you . -- Ana Resendes DVM, MSc, PhD Veterinary Pathologist Centro de Investiga??o de Recursos Naturais (CIRN) Laborat?rio de Microscopia e Histologia Departamento de Biologia, Universidade dos A?ores. Rua da M?e de Deus, 58 - Apartado 1422 P - 9501-801 Ponta Delgada (A?ores) Portugal Tel. (+351) 296 650 000 ext. 1109 Fax (+351) 296 650 100 http://www.uac.pt/~pherg/ From jennifer.l.hofecker <@t> Vanderbilt.Edu Mon Apr 6 06:21:39 2009 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Mon Apr 6 06:21:43 2009 Subject: [Histonet] Tonsil Control Blocks Needed In-Reply-To: <03E1F5968F60C5448635D49D38B283ED027DDD741C@SJMEMXMBS11.stjude.sjcrh.local> References: <03E1F5968F60C5448635D49D38B283ED027DDD741C@SJMEMXMBS11.stjude.sjcrh.local> Message-ID: <5F3F860CFE0F4741B1D87A88A58FAE9A2560AE@mailbe01.mc.vanderbilt.edu> Hi Charlene, have you considered the NSH Control Bank? The form is now online for you to request what you need and to offer what you have to share. It's a free service to NSH members and is run by the QC committee in conjunction with the IHCRG. If you have any questions, you may contact QC committee chair, William Desalvo @ wdesalvo.cac@hotmail.com. Have a great day! Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Friday, April 03, 2009 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tonsil Control Blocks Needed Fellow Histonetters, I am having an extremely hard time obtaining tonsil control tissue and was wondering if anyone would have some they could share. I would be glad to exchange some rhabdomyosarcoma tissue blocks in exchange. Please let me know if you can help. Thanks, Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105 901-595-3191 fax 901-595-3100 ________________________________ Email Disclaimer: www.stjude.org/emaildisclaimer From petepath <@t> yahoo.com Mon Apr 6 06:15:35 2009 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Mon Apr 6 06:22:20 2009 Subject: [Histonet] Entamoeba Trophozoites Message-ID: <781102.28694.qm@web45111.mail.sp1.yahoo.com> It is obviously too late for this email to be useful in this case but I will offer a suggestion for future reference. When E histolytica ?in volves the liver it typically is in the form of an abscess. Look very carefulls at the specimen and? make a wet prep smear of a small amount of tissue from anything that looks different than the normal brown appearing liver tissue. If you see anything soft or liquifactive yellowish or tan this would be ideal. Crush a speck of this tissue on a slide with a little saline and ?examine immediately with a lot of contrast. If? E. histolitica?are?present you will see these cool little buggers, not much bigger than ?a histiocyte with small cookie cutter round nuckei and?I tiny central dot crawling around the slide with purposeful motion and some containg RBCs. The encysted forms are a bit larger, rounder? and contain 4 nucleoli. If recieved in saline it would be ?worth spinning down the saline an having a look at that. You can stain the slides as discribed above?after looking at the wet ?mount.? Stephen Peters M.D.? 201 847 0052 ?Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 Phone and fax 201 847 7600 www.pathologyinnovations.com From b-frederick <@t> northwestern.edu Mon Apr 6 07:52:31 2009 From: b-frederick <@t> northwestern.edu (b-frederick@northwestern.edu) Date: Mon Apr 6 07:52:36 2009 Subject: [Histonet] Nice one Kemlo Message-ID: <20090406125231.715AE7485@merle.it.northwestern.edu> For those of you that have forgotten or never studied the french language, the female version of René would be Reneé. I'll admit to blanking out momentarily when I first joined histotnet! Bernice ==============Original message text=============== On Mon, 06 Apr 2009 2:20:18 am CDT Bernie Taupin wrote: Kemlo, So do you feel like taking a moment to address why you called me out for something even when I was correct to begin with? ________________________________ From: Kemlo Rogerson To: Bernie Taupin ; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; alan taylor Sent: Monday, April 6, 2009 3:14:35 AM Subject: RE: [Histonet] Nice one Kemlo I think the benefit of getting older SHOULD be that one gets wiser. To get old without acquiring wisdom is a waste. One of those wisdoms is to know when a conversation has ended......... or when there's no point due to the immaturity or lack of insight of some of the audience. You can use that Bernie, to stick at the end of your e-mails as a 'wise saying' (g). Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: 06 April 2009 05:28 To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; alan taylor Subject: Re: [Histonet] Nice one Kemlo 1. How is anyone supposed to know your gender online when you have such an androgynous name? 2. what does your gender have to do with this in the first place? gender equality is gender equality. but, not surprisingly, none of the feminists on this list have come out to say that. though i bet some would if i said something sexist, eh? ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; alan taylor Sent: Saturday, April 4, 2009 1:01:36 PM Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am "a legitimate April Fool" and I intend to keep doing what I am doing for as long as I can. René J. --- On Sat, 4/4/09, alan taylor wrote: From: alan taylor Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ===========End of original message text=========== From SwainFrancesL <@t> uams.edu Mon Apr 6 07:56:23 2009 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Mon Apr 6 07:57:05 2009 Subject: [Histonet] unsubscribe Message-ID: Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From vazquezr <@t> ohsu.edu Mon Apr 6 08:04:54 2009 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon Apr 6 08:05:08 2009 Subject: [Histonet] Nice one Kemlo In-Reply-To: <658345.87226.qm@web65701.mail.ac4.yahoo.com> References: <58F70F8C715A45D0AB2E64C806B81D43@merlin> <658345.87226.qm@web65701.mail.ac4.yahoo.com> Message-ID: <2A582E8156B45F468A62D1F1D20AF0837143D9@EX-BE08.ohsu.edu> Bravo Rene...I learn A LOT from you and your "know it all" vast knowledge, as well as all the knowledgeable people on this list. Bernie, get a life and quit being just down rite mean. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, April 04, 2009 10:02 AM To: histonet@lists.utsouthwestern.edu; alan taylor Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan?for your nice words, only a detail though: I am a HE and not a SHE?that just?turned 75 on April First, I am "a legitimate April Fool" and I intend to keep doing what I am doing for as long as I can. Ren? J. --- On Sat, 4/4/09, alan taylor wrote: From: alan taylor Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Mon Apr 6 08:07:45 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon Apr 6 08:09:14 2009 Subject: [Histonet] Removing PMMA blocks from glass vials References: <4EBFF65383B74D49995298C4976D1D5E03835CAB@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F298B@fhosxchmb006.ADVENTISTCORP.NET> Use a razor blade to slit one side of the vial lengthwise and pull apart. Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pat Flannery Sent: Fri 4/3/2009 5:33 PM To: Histonet Subject: Re: [Histonet] Removing PMMA blocks from glass vials OK, silly question time: How do you get them out of *plastic* vials? I've always used the glass scint vials,too. Doesn't the PMMA melt plastic vials (or stick too well to them)? -Pat Flannery Duke Med On Apr 3, 2009, at 2:00 PM, "Monfils, Paul" wrote: > Someone gave me some small PMMA blocks polymerized inside glass > scintillation vials. I always use plastic vials. Obviously the > vials have to be broken to get the blocks out. Does anyone have > some advice on the best way to do this? The guy who brought them > said he thought I could "use liquid nitrogen", but had no > specifics. Any help will be appreciated. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Janet.Bonner <@t> FLHOSP.ORG Mon Apr 6 08:09:44 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon Apr 6 08:12:24 2009 Subject: [Histonet] cytology screening requirements References: <000c01c9b61f$f7e8f290$e7bad7b0$@osborn@comcast.net> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F298C@fhosxchmb006.ADVENTISTCORP.NET> Dear All, The more I see Histologists who worked one-on-one with a Pathologist/Doctor, the more I see a group of concerned individuals who think out-of-the-box, and they have been extremely enriched by the experience. The new students who have not had this experience have missed out on a wonderful facet of Histology! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sharon Osborn Sent: Sun 4/5/2009 2:54 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] cytology screening requirements Terri and Allysa, Terri, just reading what you have written about CLIA '88 regs for Cytology screening reminds me why I no longer do cytology and just do histology. I did both for over 15 years, leaving several years prior to CLIA 88. I worked in rural area designated as a regional medical center. We had two pathologists, 2 histotechs, 1 cyto tech(me) plus I was backup histo. I did ALL the cytology prep work, staining, cover slipping, screening, filing and ordering. The secretary did the recording and the billing. My work was very interesting for I saw it all in fluids and Paps. We had some very progressive specialist clinicians so that when needle aspirates first came out, we were doing them. My pathologists' attitude was that any meeting/training was worth it if we learned one thing well and implemented it. They would challenge me and I would challenge them on new technics, ideas and best health care practice. Some days a person can screen more slides while other days screen less. This can be due to the type/quality of the slide, or a number of different variables. This was the days before Thin Prep when the spatula was used to scrap the cervix. The quality of the slides depended upon the skill of the clinician or whomever was doing the scraping. My pathologists did a 10% rescreen of my work before it was a requirement because we felt that was the correct process for continuing evaluation. Sharon Osborn IHC-QC ThermoFisherScientific Fremont, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From cathy <@t> wasatchhisto.com Mon Apr 6 08:40:17 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Mon Apr 6 08:40:49 2009 Subject: [Histonet] small equipment for sale Message-ID: 2 600 gram balances for sale, both were certified last November, also have certified weight set 1 slide dryer with cover, we used at one time for paraffin sectioning 2 Premiere microscopes, used for QC of GMA sections at the time of sectioning, has 4x, 10x, 20x and 30x and 40x 1 Leica DME dual head microscope has 4x, 10x, 20x and 30x and 40x, used for QC of staining and final QC of slides Cathy Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 From cathy <@t> wasatchhisto.com Mon Apr 6 09:24:21 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Mon Apr 6 09:24:53 2009 Subject: [Histonet] 5 gal MMA for sale Message-ID: <9D910591674D4408AE99492B27DFBAF1@shop1e2e996aa5> I have a sealed 5 gallon can of Aldrich MMA for sale for $50 plus shipping. We also have 12 or 13 gallons of Stat Lab Reagent Alcohol. Cathy A. Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 From cathy <@t> wasatchhisto.com Mon Apr 6 10:12:33 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Mon Apr 6 10:13:01 2009 Subject: [Histonet] light box for sale Message-ID: I have a light box for $50 plus shipping and a nice Shandon paraffin embedding center. Cathy A. Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 From mcauliff <@t> umdnj.edu Mon Apr 6 10:34:38 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Apr 6 10:34:58 2009 Subject: [Histonet] Problems with mouse brains In-Reply-To: <040346FA7309BD439C327F97D4C4D69B04A1C481@ISIS.fhcrc.org> References: <040346FA7309BD439C327F97D4C4D69B04A1C481@ISIS.fhcrc.org> Message-ID: <49DA210E.8000101@umdnj.edu> Hi Julie: You mentioned that you are buying your fixative from a commercial supplier but that "My first thought is that there was difference in formalin... but that is not the case." How do you know? You did not make the fixative solution so you really have no idea. Not to berate Fisher Scientific, I have been a loyal customer since grad school. Maybe someone had a bad day or was interrupted during the mixing. Mixing buffered formalin in you lab is so easy and you retain control of the most important step in histology. Geoff Randolph-Habecker, Julie wrote: > Folks, > > I need some input on a problem we're seeing with some mouse brain > samples. The samples are from new born mice P0 to P14 which have been > fixed in 10% NBF (Fisher brand and well within expiration date) for 72 > hours. They are then transferred to 70% etoh and processed on an 8 hour > process. After the tissue is embedded, we are taking sagital sections, > drying them overnight, and then baking overnight at 60C. They are then > stained with H&E. This has been working great but now all of a sudden we > have very light H&E staining, nuclear bubbling, and extensive tissue > cracking. I also have noticed some formalin pigment. > > My first thought is that there was difference in formalin or time in > formalin but that is not the case. I also wondered if the tissue might > be exposed to excessive heat. We checked all of the temperatures in our > processor, embedding center, and water baths - all were within > tolerance. > > Any ideas what might cause all three artifacts? Could it be from > inadequate paraffin infiltration? > > Any help would be greatly appreciated! > > Thanks, > > Julie > > Julie Randolph-Habecker, Ph.D. > Staff Scientist - Director > Experimental Histopathology Shared Resource > Fred Hutchinson Cancer Research Center > 1100 Fairview Ave, N. DE-360 (Please note new location) > Seattle WA 98109-1024 > 206-667-6119 > jhabecke@fhcrc.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From sbreeden <@t> nmda.nmsu.edu Mon Apr 6 10:43:49 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Apr 6 10:43:55 2009 Subject: [Histonet] I'm outta here Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E67C1@nmdamailsvr.nmda.ad.nmsu.edu> After almost 5 years of Histonet membership, I am taking a break and unsubscribing. Why? Because I've had enough of the extraneous nonsense. I'll unsubscribe as required but I'm telling you why you won't be coming into this computer for a while. This listserv has so much potential to do good but some are just like the gangsta wannabees that use their smarts for crap. If I need help, I'll hit the books. Ta-ta for now. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From cathy <@t> wasatchhisto.com Mon Apr 6 12:04:08 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Mon Apr 6 12:04:34 2009 Subject: [Histonet] glassware + Tissue Tek II staining trays Message-ID: <828D064AEC31431D94C9B21A4D123DCB@shop1e2e996aa5> I have glass dishes, glass staining racks, beakers, flasks and 3 Tissue Tek II staining trays. I also have lots of the slide holders for the Tissue Tek II staining trays. Cathy A. Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 www.wasatchhisto.com From MLafrini <@t> csmlab.com Mon Apr 6 12:58:20 2009 From: MLafrini <@t> csmlab.com (Michael LaFriniere) Date: Mon Apr 6 12:59:00 2009 Subject: [Histonet] Question: Slides/Day? In-Reply-To: <76452FD25660487796C8533613565A8D@wchsys.org> References: <76452FD25660487796C8533613565A8D@wchsys.org> Message-ID: <49DA0A7D.588C.00AF.0@csmlab.com> Alyssa, As Director of a large AP lab here in Maryland, to answer your question to the best ability, one would need additional information. Currently under accreditation guidelines a Cytotechnologist is "allowed to read "100 non imaged" slides per 24 hour period, or 200 {negative} "imaged" slides per 24 hour period [GYN cases]...however, I find this not practical in the "real world" to include probably not the best practice for high quality patient care... THIS IS ONLY MY OPINION with the 25 years + of experience I encompass....In my lab I like to have a Cytotechnologist with 5+ years of experience reading up to 75-85 slides that are non imaged and 120-140 slides that are imaged. Our quality indicators demonstrate these are the levels that produce the best productivity while maintaining high quality Assurance of patient care.. our lab does have high retainment of Cytotechnologists, in fact I have a waiting list of Cytotech's for openings. I pay less then the 75% percentile for Cytotech wages nation wide, however, I offer a daily flex schedule to allow the Cytotech to come in anytime throughout the day to work their 8-9 hours they feel at their best to meet their production standard and the expectation of maintaining a low FNP. Non GYN's fall under the non imaged category and screening times are dependant on the type of case.... Hope this helps Michael Michael R. LaFriniere Executive Director Cytology Services of Maryland (CSM) 301-206-2555 ext 27 301-206-2595 fax michael.lafriniere@csmlab.com >>> On 4/2/2009 at 4:38 PM, "Joyce Cline" wrote: Our cytotechs screen 40 to 60 a day. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alyssa Peterson Sent: Thursday, April 02, 2009 3:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question: Slides/Day? Hello Histonetters, I understand most of the professionals on this website are histology professionals, however, I thought I would give it a try since I have not found a cytology listserv yet. Does anyone know how many slides per day a cytotechnologist would screen within a private lab setting (On average)? -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lldewe <@t> gmail.com Mon Apr 6 12:59:03 2009 From: lldewe <@t> gmail.com (Loralei Dewe) Date: Mon Apr 6 12:59:06 2009 Subject: [Histonet] Free or inexpensive histology items Message-ID: <7173d3c00904061059h366c77efl1805abe1120e850d@mail.gmail.com> Hi All, I have seen alot of posts recently with folks who are selling histology items very inexpensively or free. I am trying to set up a small histology lab in Northern Calif for vets in the area to get their animal tissues processed without having to send them to large and very pricey processing labs. I can use any/all gently used items I can get my hands on! Thank you in advance!! Loralei Dewe From cathy <@t> wasatchhisto.com Mon Apr 6 13:30:23 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Mon Apr 6 13:30:50 2009 Subject: [Histonet] paraffin slides for sale Message-ID: <69C248470F36403FADBEE281B02E96F0@shop1e2e996aa5> Fisher Scientific slides @ 2 $2/box 20 boxes green 5 boxes yellow Premiere brand 20 boxes green 8 boxes white Cathy A. Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 www.wasatchhisto.com From Charlene.Henry <@t> STJUDE.ORG Mon Apr 6 13:57:21 2009 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Mon Apr 6 13:57:25 2009 Subject: [Histonet] Thanks for Response to Tonsil Controls Needed Message-ID: <03E1F5968F60C5448635D49D38B283ED027DDD743C@SJMEMXMBS11.stjude.sjcrh.local> I want to thank all of you that have reached out and offered tonsil control to our laboratory. I have blocks that are now being sent to me and I think that I will will have plenty to last for a while. You guys are the greatest!!! Again Thanks, Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105 901-595-3191 fax 901-595-3100 ________________________________ Email Disclaimer: www.stjude.org/emaildisclaimer From akemiat3377 <@t> yahoo.com Mon Apr 6 14:41:57 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Mon Apr 6 14:42:01 2009 Subject: [Histonet] Sara Sorry to see you go Message-ID: <453626.30821.qm@web31305.mail.mud.yahoo.com> Hi Sara, I for one will be sad to see your absence. ?Perhaps in the future, you will reconsider and join our group again. ?It is extremely unfortunate to see these grown men acting like FOOLS! ? I have been pretty much a?voyeur?as of late, unless I have something credible to say or ask, but I feel the urge to state my feelings as a fellow histologist. ?And by the way, there are a number of extremely prominent histologists and pathologists that have stated they are voyeurs too! ?They may not go on line, but they see your postings! ?They have all taken note of this stupid on-line communication. ?I don't think these individuals realize that the pathology community observes these communications, and this may come back to bite them in the butt later on down the line! ?It lowers the image of these individuals as?professionals,?and their names and?verbiage?are forever archived in our memories. ?"Once you ring the bell, you can't un-ring it"! The Histonet Forum was initially created and funded by histology vendors and concerned histologists to help us as a group. ?I maybe wrong, but I thought it's purpose was to expand our knowledge in this valuable profession. ?I have been utilizing this wonderful tool, since almost the beginning. ?It was an eye opening experience when I started seeing pathologists joining in. ?It made me realize that they too saw the merit in this wonderful tool for sharing our knowledge and experiences. ? I have been involved in histology since taking classes in histomicrotechnic at Tokyo University in 1968. ?The older I get, the more I realize how little I know. ?Especially with the use of molecular biology, and more and more esoteric testing. ?We are experiencing a metamorphosis in our field, and forums such as this, are extremely valuable! Those of us who use this tool, do so, because we care about giving the best possible patient care.??At least I use to think so........ This is a wonderful tool for histologists, researchers, and concerned pathologists, to come together for the sole purpose to learn and grow?(then there's the Friday good hearted release of steam....) ?The Histonet is especially important for those individuals who are geographically isolated, and do not have the means to attend continuing education classes.? It is my belief that the Histonet was never intended to be used as a tool as these individuals have so irresponsibly done. ?It is childish and unprofessional. ?This sort of use just shows how unthinking and selfish these individuals are. ?I am ashamed that the pathology community sees this sort of communication between histologists. ?It demeans us as a whole. ?I don't think these men realize what fallout may occur. ?We may have this taken away from us because they have not used their good judgement, common sense, and restraint! ?I realize that in the past, I too may have spoken a little out of turn, but this thread has become an?embarrassment. I have recently accepted a employment opportunity to go back to managing a histology lab, and will ask my fellow histologists for their assistance. ?I?truly?hope there will be a Histonet available when I need you! Let's hope we can learn from this experience and move on......... ? Regards,Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, & TMA Tele: (425) 941-4287 E-Mail: akemiat3377@yahoo.com --- On Mon, 4/6/09, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] I'm outta here To: histonet@lists.utsouthwestern.edu Date: Monday, April 6, 2009, 8:43 AM After almost 5 years of Histonet membership, I am taking a break and unsubscribing. Why? Because I've had enough of the extraneous nonsense. I'll unsubscribe as required but I'm telling you why you won't be coming into this computer for a while.? This listserv has so much potential to do good but some are just like the gangsta wannabees that use their smarts for crap.? If I need help, I'll hit the books.? Ta-ta for now. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lactose.intolerant <@t> yahoo.com Mon Apr 6 15:02:01 2009 From: lactose.intolerant <@t> yahoo.com (aa aa) Date: Mon Apr 6 15:02:06 2009 Subject: [Histonet] I'm outta here In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E67C1@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <8952.94524.qm@web55305.mail.re4.yahoo.com> This, ladies and gentlemen, is a textbook example of a "flounce"... you know, that "last post" to tell a bunch of strangers that you're no longer participating on the list... and as expected, sympathetic/apologetic replies to that from others... http://encyclopediadramatica.com/Flounce p.s: thanks also for posting an off-topic post to tell us you're quitting hte list because of too much off-topic content.... --- On Mon, 4/6/09, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] I'm outta here To: histonet@lists.utsouthwestern.edu Date: Monday, April 6, 2009, 11:43 AM After almost 5 years of Histonet membership, I am taking a break and unsubscribing. Why? Because I've had enough of the extraneous nonsense. I'll unsubscribe as required but I'm telling you why you won't be coming into this computer for a while. This listserv has so much potential to do good but some are just like the gangsta wannabees that use their smarts for crap. If I need help, I'll hit the books. Ta-ta for now. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jflinn <@t> gmu.edu Mon Apr 6 15:12:45 2009 From: jflinn <@t> gmu.edu (Jane M Flinn) Date: Mon Apr 6 15:12:49 2009 Subject: [Histonet] I'm outta here In-Reply-To: <8952.94524.qm@web55305.mail.re4.yahoo.com> References: <4D14F0FC9316DD41972D5F03C070908B017E67C1@nmdamailsvr.nmda.ad.nmsu.edu> <8952.94524.qm@web55305.mail.re4.yahoo.com> Message-ID: I don't agree, I have stayed out of this whole thing. I just delete the emails without reading them. I stay with histonet because there is good stuff from time to time. But I have never seen so many time wasting e mails. jane "Life is short - make haste to be kind" Dr. Jane Flinn Director, Undergraduate Neuroscience Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: aa aa Date: Monday, April 6, 2009 4:02 pm Subject: Re: [Histonet] I'm outta here > This, ladies and gentlemen, is a textbook example of a > "flounce"... you know, that "last post" to tell a bunch of > strangers that you're no longer participating on the list... and > as expected, sympathetic/apologetic replies to that from others... > > http://encyclopediadramatica.com/Flounce > > p.s: thanks also for posting an off-topic post to tell us you're > quitting hte list because of too much off-topic content.... > > --- On Mon, 4/6/09, Breeden, Sara wrote: > From: Breeden, Sara > Subject: [Histonet] I'm outta here > To: histonet@lists.utsouthwestern.edu > Date: Monday, April 6, 2009, 11:43 AM > > After almost 5 years of Histonet membership, I am taking a break and > unsubscribing. Why? Because I've had enough of the extraneous > nonsense.I'll unsubscribe as required but I'm telling you why you > won't be > coming > into this computer for a while. This listserv has so much > potential to > do good but some are just like the gangsta wannabees that use their > smarts for crap. If I need help, I'll hit the books. Ta-ta for now. > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From HornHV <@t> archildrens.org Mon Apr 6 15:18:12 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Apr 6 15:18:18 2009 Subject: [Histonet] email address Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83116@EMAIL.archildrens.org> Does anyone have Dr. Margraf or Dr. Hagler's email address?? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From dlschneider <@t> gmail.com Mon Apr 6 15:26:02 2009 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Mon Apr 6 15:26:07 2009 Subject: Moderator? Re: [Histonet] I'm outta here Message-ID: <1085e7000904061326q2e42c94djbefbb4968c756545@mail.gmail.com> Is there a list moderator? From JWeems <@t> sjha.org Mon Apr 6 15:37:07 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Apr 6 15:37:10 2009 Subject: [Histonet] Let's call a truce In-Reply-To: <49D490EC.F783.00DA.0@childrens.com> References: <786753.18109.qm@web46112.mail.sp1.yahoo.com><445593.70778.qm@web43511.mail.sp1.yahoo.com> <49D490EC.F783.00DA.0@childrens.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA542EE68@ITSSSXM01V6.one.ads.che.org> Dr. Margraf asked us to stop last Thurs... It's time now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Thursday, April 02, 2009 11:18 AM To: HistoNet; Bernie Taupin Subject: Re: [Histonet] Let's call a truce Histonet members: As the list moderator, I now feel the need to ask everyone to please get back to the business of histology and related topics. Histonet is run using the University of Texas Southwestern Medical Center's resources and I suspect they would not look favorably on large volumes of off-topic messages. As everyone knows, Histonet is a very useful resource because of the tremendous contributions of all the members (now up to 3200 people) and it would be a shame if a University official happened to review the recent discourse and decide this wasn't a good use of Texas tax dollars. Thanks so much, Linda M Histonet administrator Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From akemiat3377 <@t> yahoo.com Mon Apr 6 16:25:45 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Mon Apr 6 16:25:49 2009 Subject: Moderator? Re: [Histonet] I'm outta here Message-ID: <741464.6244.qm@web31307.mail.mud.yahoo.com> Hi Sara, I for one will be sad to see your absence. ?Perhaps in the future, you will reconsider and join our group again. ?It is extremely unfortunate to see these grown men acting like FOOLS! ? I have been pretty much a?voyeur?as of late, unless I have something credible to say or ask, but I feel the urge to state my feelings as a fellow histologist. ?And by the way, there are a number of extremely prominent histologists and pathologists that have stated they are voyeurs too! ?They may not go on line, but they see your postings! ?They have all taken note of this stupid on-line communication. ?I don't think these individuals realize that the pathology community observes these communications, and this may come back to bite them in the butt later on down the line! ?It lowers the image of these individuals as?professionals,?and their names and?verbiage?are forever archived in our memories. ?"Once you ring the bell, you can't un-ring it"! The Histonet Forum was initially created and funded by histology vendors and concerned histologists to help us as a group. ?I maybe wrong, but I thought it's purpose was to expand our knowledge in this valuable profession. ?I have been utilizing this wonderful resource, since almost the beginning. ?It was an eye opening experience when I started seeing pathologists joining in. ?It made me realize that they too saw the merit in this wonderful?resource,?for sharing our knowledge and experiences. ? I have been involved in histology since taking classes in histomicrotechnic at Tokyo University in 1968. ?The older I get, the more I realize how little I know. ?Especially with the use of molecular biology, and more and more esoteric testing. ?We are experiencing a metamorphosis in our field, and forums such as this, are extremely valuable! Those of us who use this tool, do so, because we care about giving the best possible patient care.??At least I use to think so........ This is a wonderful?resource,?for histologists, researchers, and concerned pathologists, to come together for the sole purpose to learn and grow?(then there's the Friday good hearted release of steam....) ?The Histonet is especially important for those individuals who are geographically isolated, and do not have the means to attend continuing education classes.? It is my belief that the Histonet was never intended to be used in this manner. ?These individuals have used this?resource?irresponsibly. ?It is childish and unprofessional. ?This sort of use just shows how unthinking and selfish these individuals are. ?I am ashamed that the pathology community sees this sort of communication between histologists. ?It demeans us as a whole. ?I don't think these men realize what fallout may occur. ?We may have this taken away from us because they have not used their good judgement, common sense, and restraint! ? I realize that in the past, I too may have spoken a little out of turn, but this thread has become an?embarrassment. I have recently accepted a employment opportunity to go back to managing a histology lab, and will ask my fellow histologists for their assistance. ?I?truly?hope there will be a Histonet available when I need you! Let's hope we can learn from this experience and move on......... ? Regards,Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, & TMA Tele: (425) 941-4287 E-Mail: akemiat3377@yahoo.com --- On Mon, 4/6/09, Daniel Schneider wrote: From: Daniel Schneider Subject: Moderator? Re: [Histonet] I'm outta here To: histonet@lists.utsouthwestern.edu Date: Monday, April 6, 2009, 1:26 PM Is there a list moderator? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Mon Apr 6 17:10:03 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Mon Apr 6 17:11:36 2009 Subject: [Histonet] "FREEZY" spray References: Message-ID: Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it! Claire ________________________________ Federal registry 1910.1030 "All procedures involving blood or other potential infectious materials shall be performed in such a manner as to minimize splashing, spraying, spattering, or generation of droplets of these substances." NCCLS Document M29-T (now CLSI) "Frozen sections done on unfixed tissue pose a high risk because accidents are common. Freezing of tissue does not inactivate infectious agents. Freezing propellants under pressure should not be used for frozen sections as they may cause spattering of droplets of infectious material." Based on the above statements, freezing spray should NOT be used in a cryostat with unfixed tissue. Jennifer MacDonald From JMacDonald <@t> mtsac.edu Mon Apr 6 17:34:03 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Apr 6 17:35:46 2009 Subject: [Histonet] "FREEZY" spray In-Reply-To: Message-ID: Based on my limited knowledge of liquid nitrogen I would say no. Is it used with a propellant? "Ingles Claire " Sent by: histonet-bounces@lists.utsouthwestern.edu 04/06/2009 03:13 PM To undisclosed-recipients:; cc Histonet@lists.utsouthwestern.edu Subject RE: [Histonet] "FREEZY" spray Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it! Claire ________________________________ Federal registry 1910.1030 "All procedures involving blood or other potential infectious materials shall be performed in such a manner as to minimize splashing, spraying, spattering, or generation of droplets of these substances." NCCLS Document M29-T (now CLSI) "Frozen sections done on unfixed tissue pose a high risk because accidents are common. Freezing of tissue does not inactivate infectious agents. Freezing propellants under pressure should not be used for frozen sections as they may cause spattering of droplets of infectious material." Based on the above statements, freezing spray should NOT be used in a cryostat with unfixed tissue. Jennifer MacDonald _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Mon Apr 6 17:37:04 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Mon Apr 6 17:37:10 2009 Subject: [Histonet] Sara Sorry to see you go In-Reply-To: <453626.30821.qm@web31305.mail.mud.yahoo.com> References: <453626.30821.qm@web31305.mail.mud.yahoo.com> Message-ID: <109670.4268.qm@web43505.mail.sp1.yahoo.com> This is a great example of the sort of missive that could more appropriately have been sent privately, off-list, yeah? ________________________________ From: Akemi Allison-Tacha To: histonet@lists.utsouthwestern.edu; SaraBreeden Sent: Monday, April 6, 2009 3:41:57 PM Subject: [Histonet] Sara Sorry to see you go Hi Sara, I for one will be sad to see your absence. Perhaps in the future, you will reconsider and join our group again. It is extremely unfortunate to see these grown men acting like FOOLS! I have been pretty much a voyeur as of late, unless I have something credible to say or ask, but I feel the urge to state my feelings as a fellow histologist. And by the way, there are a number of extremely prominent histologists and pathologists that have stated they are voyeurs too! They may not go on line, but they see your postings! They have all taken note of this stupid on-line communication. I don't think these individuals realize that the pathology community observes these communications, and this may come back to bite them in the butt later on down the line! It lowers the image of these individuals as professionals, and their names and verbiage are forever archived in our memories. "Once you ring the bell, you can't un-ring it"! The Histonet Forum was initially created and funded by histology vendors and concerned histologists to help us as a group. I maybe wrong, but I thought it's purpose was to expand our knowledge in this valuable profession. I have been utilizing this wonderful tool, since almost the beginning. It was an eye opening experience when I started seeing pathologists joining in. It made me realize that they too saw the merit in this wonderful tool for sharing our knowledge and experiences. I have been involved in histology since taking classes in histomicrotechnic at Tokyo University in 1968. The older I get, the more I realize how little I know. Especially with the use of molecular biology, and more and more esoteric testing. We are experiencing a metamorphosis in our field, and forums such as this, are extremely valuable! Those of us who use this tool, do so, because we care about giving the best possible patient care. At least I use to think so........ This is a wonderful tool for histologists, researchers, and concerned pathologists, to come together for the sole purpose to learn and grow (then there's the Friday good hearted release of steam....) The Histonet is especially important for those individuals who are geographically isolated, and do not have the means to attend continuing education classes. It is my belief that the Histonet was never intended to be used as a tool as these individuals have so irresponsibly done. It is childish and unprofessional. This sort of use just shows how unthinking and selfish these individuals are. I am ashamed that the pathology community sees this sort of communication between histologists. It demeans us as a whole. I don't think these men realize what fallout may occur. We may have this taken away from us because they have not used their good judgement, common sense, and restraint! I realize that in the past, I too may have spoken a little out of turn, but this thread has become an embarrassment. I have recently accepted a employment opportunity to go back to managing a histology lab, and will ask my fellow histologists for their assistance. I truly hope there will be a Histonet available when I need you! Let's hope we can learn from this experience and move on......... Regards,Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL President Phoenix Lab Consulting Specializing in Histology, SS, IHC, & TMA Tele: (425) 941-4287 E-Mail: akemiat3377@yahoo.com --- On Mon, 4/6/09, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] I'm outta here To: histonet@lists.utsouthwestern.edu Date: Monday, April 6, 2009, 8:43 AM After almost 5 years of Histonet membership, I am taking a break and unsubscribing. Why? Because I've had enough of the extraneous nonsense. I'll unsubscribe as required but I'm telling you why you won't be coming into this computer for a while. This listserv has so much potential to do good but some are just like the gangsta wannabees that use their smarts for crap. If I need help, I'll hit the books. Ta-ta for now. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Mon Apr 6 17:43:07 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Mon Apr 6 17:43:11 2009 Subject: [Histonet] Nice one Kemlo In-Reply-To: <185260.57134.qm@web65713.mail.ac4.yahoo.com> References: <185260.57134.qm@web65713.mail.ac4.yahoo.com> Message-ID: <453473.65848.qm@web43516.mail.sp1.yahoo.com> > Instead of spitting your venom try to learn Sort of like you might consider learning correct English? I mean, its one thing if its not your first language, but despite how many people on here called you kindly and helpful, I find it perplexing that you feel compelled to call me a "piece of ignorant", and TWICE, no less. I do not speak French. This list is not in French. Ergo, my awareness (or lack thereof) of proper French diction has absolutely nothing at all to do with anything... aside form providing you a vehicle to call me names and lash out. If you have something to say, say it. The simple fact that you do not like me does not give you- or anyone else- wholesale right to insult me or call me names. You should be ashamed of yourself, Rene. I never expected such antisocial behavior from you, and frankly, when you behave so regrettably, I feel no compulsion to "learn" anything form you! ________________________________ From: Rene J Buesa To: Bernie Taupin Sent: Monday, April 6, 2009 9:40:18 AM Subject: Re: [Histonet] Nice one Kemlo My name (Ren?) is not androginous you piece of ignorant. It just shows that you don't know anything about names. Ask any French speaking person about my name or the one reseved for females (Ren?e) and they can tell you that there is no confusion possible, you ignorant. Instead of spitting your venom try to learn Ren? J. --- On Mon, 4/6/09, Bernie Taupin wrote: From: Bernie Taupin Subject: Re: [Histonet] Nice one Kemlo To: rjbuesa@yahoo.com, histonet@lists.utsouthwestern.edu, "alan taylor" Date: Monday, April 6, 2009, 12:27 AM 1. How is anyone supposed to know your gender online when you have such an androgynous name? 2. what does your gender have to do with this in the first place? gender equality is gender equality. but, not surprisingly, none of the feminists on this list have come out to say that. though i bet some would if i said something sexist, eh? ________________________________ From: Rene J Buesa To: histonet@lists.utsouthwestern.edu; alan taylor Sent: Saturday, April 4, 2009 1:01:36 PM Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am "a legitimate April Fool" and I intend to keep doing what I am doing for as long as I can. Ren? J. --- On Sat, 4/4/09, alan taylor wrote: From: alan taylor Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Mon Apr 6 17:44:14 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Mon Apr 6 17:44:19 2009 Subject: [Histonet] paraffin slides for sale In-Reply-To: <69C248470F36403FADBEE281B02E96F0@shop1e2e996aa5> References: <69C248470F36403FADBEE281B02E96F0@shop1e2e996aa5> Message-ID: <30749.39966.qm@web43503.mail.sp1.yahoo.com> Parrafin slides? Does that mean they melt when they go into xylene?? I prefer glass slides, if you ask me.... ________________________________ From: Cathy Mayton To: histonet@lists.utsouthwestern.edu Sent: Monday, April 6, 2009 2:30:23 PM Subject: [Histonet] paraffin slides for sale Fisher Scientific slides @ 2 $2/box 20 boxes green 5 boxes yellow Premiere brand 20 boxes green 8 boxes white Cathy A. Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 www.wasatchhisto.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Mon Apr 6 17:46:59 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Mon Apr 6 17:47:08 2009 Subject: [Histonet] Nice one Kemlo In-Reply-To: <2A582E8156B45F468A62D1F1D20AF0837143D9@EX-BE08.ohsu.edu> References: <58F70F8C715A45D0AB2E64C806B81D43@merlin> <658345.87226.qm@web65701.mail.ac4.yahoo.com> <2A582E8156B45F468A62D1F1D20AF0837143D9@EX-BE08.ohsu.edu> Message-ID: <82217.66816.qm@web43513.mail.sp1.yahoo.com> > Bernie, get a life and quit being just down rite mean. Yes, Robyn... because you telling me to "get a life" isn't mean at all! Do any of you practice what you preach? Or do you all just yell at other people online while continuing to do the very thing yourself that you were yelling at them about doing? ________________________________ From: Robyn Vazquez To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; alan taylor Sent: Monday, April 6, 2009 9:04:54 AM Subject: RE: [Histonet] Nice one Kemlo Bravo Rene...I learn A LOT from you and your "know it all" vast knowledge, as well as all the knowledgeable people on this list. Bernie, get a life and quit being just down rite mean. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, April 04, 2009 10:02 AM To: histonet@lists.utsouthwestern.edu; alan taylor Subject: [Histonet] Nice one Kemlo Thanks to Kenlo and Alan for your nice words, only a detail though: I am a HE and not a SHE that just turned 75 on April First, I am "a legitimate April Fool" and I intend to keep doing what I am doing for as long as I can. Ren? J. --- On Sat, 4/4/09, alan taylor wrote: From: alan taylor Subject: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Date: Saturday, April 4, 2009, 12:42 PM Nice one Kemlo: Rene has always impressed me with the depth and breadth of her obviously great knowledge and skill in the practice of our humble art. Long may she continue to do so. As a histo group we should really strive to help each other and share our many and unique skills and applied techniques. A Giemsa squash prep for seeking Entamoeba, as described, sounds very appropriate to me. Come on guys, there has been a lot of knocking and critiscm of colleagues online lately. Now is the time to stop. Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. Devon. England. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Mon Apr 6 17:48:00 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Mon Apr 6 17:48:04 2009 Subject: [Histonet] (no subject) In-Reply-To: <24F24A72BE11F2409C0613BAD15A496B45823B@MAILC.wmhs.com> References: <24F24A72BE11F2409C0613BAD15A496B45823B@MAILC.wmhs.com> Message-ID: <768749.17646.qm@web43505.mail.sp1.yahoo.com> Lois, do you feel like typing your reply in ALL CAPS helps get your point across better? Many folks consider ALL CAPS to be the inline equivalent of yelling. So, are you a yeller? ________________________________ From: "Cover, Lois" To: histonet@lists.utsouthwestern.edu Sent: Monday, April 6, 2009 5:42:00 AM Subject: [Histonet] (no subject) I HAVE SUBSCRIBED TO THE HISTONET DIGEST TO GAIN VALUABLE INFORMATION FROM MY PEERS. I THINK IT WOULD BE BEST IF THOSE WHO HAVE ISSUES WITH EACH OTHER "TALK" OFF-LINE. I DON'T WANT TO SPEND VALUABLE TIME READING THIS ENDLESS NONSENSE! Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbritten <@t> aol.com Mon Apr 6 17:56:33 2009 From: tbritten <@t> aol.com (tbritten@aol.com) Date: Mon Apr 6 17:53:02 2009 Subject: [Histonet] Re: clinical immunology or microbiology blog sites? Message-ID: <1687944666-1239058378-cardhu_decombobulator_blackberry.rim.net-2105997372-@bxe1007.bisx.prod.on.blackberry> Hello all; I have over the last several years learned much from you all. I'm involved also in the above areas. Can any of you suggest blog sites for these disciplines simliar to this site? Best regards, tom britten ------Original Message------ From: Cathy Mayton Sender: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Apr 6, 2009 9:40 AM Subject: [Histonet] small equipment for sale 2 600 gram balances for sale, both were certified last November, also have certified weight set 1 slide dryer with cover, we used at one time for paraffin sectioning 2 Premiere microscopes, used for QC of GMA sections at the time of sectioning, has 4x, 10x, 20x and 30x and 40x 1 Leica DME dual head microscope has 4x, 10x, 20x and 30x and 40x, used for QC of staining and final QC of slides Cathy Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent from my Verizon Wireless BlackBerry From AnthonyH <@t> chw.edu.au Mon Apr 6 18:07:40 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Apr 6 18:07:48 2009 Subject: [Histonet] I'm outta here In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E67C1@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: But just use the delete key. I do Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, 7 April 2009 1:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I'm outta here After almost 5 years of Histonet membership, I am taking a break and unsubscribing. Why? Because I've had enough of the extraneous nonsense. I'll unsubscribe as required but I'm telling you why you won't be coming into this computer for a while. This listserv has so much potential to do good but some are just like the gangsta wannabees that use their smarts for crap. If I need help, I'll hit the books. Ta-ta for now. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Barry.R.Rittman <@t> uth.tmc.edu Mon Apr 6 18:16:08 2009 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon Apr 6 18:16:11 2009 Subject: [Histonet] Histology Message-ID: <75A0543E23D3A7458012D9E02EDBEC0002E806CFED@UTHCMS1.uthouston.edu> I hate to bring up histological technique for a change of pace but........... Could we get some consensus of opinion as to maximum, minimum and optimal fixation times for different tissues? This is assuming that tissues will be fixed in buffered formalin at room temperature and processed to wax with a standard technique in a processor. This would also require a standard thickness for each tissue type. If there are students out there looking for projects this might seem to be suitable, as a few tissues only could be examined at one time. I know that several papers have been published about fixation in formalin but can't bring to mind any that deal with this aspect of the topic. If there are any students out there who would like a summary of fixation in general I will be happy to email it to them. Barry From jwatson <@t> gnf.org Mon Apr 6 18:23:03 2009 From: jwatson <@t> gnf.org (James Watson) Date: Mon Apr 6 18:25:00 2009 Subject: [Histonet] Histology In-Reply-To: <75A0543E23D3A7458012D9E02EDBEC0002E806CFED@UTHCMS1.uthouston.edu> References: <75A0543E23D3A7458012D9E02EDBEC0002E806CFED@UTHCMS1.uthouston.edu> Message-ID: Formaldehyde Fixation0022-1554/85/83. 30. The. Journal of Histochemistry and Cytochemistry. Copyright ... U.S.A.. Formaldehyde. Fixation. Review. Article. CECIL. H. FOX, ... www.jhc.org/cgi/reprint/33/8/845.pdf - Similar pages by CH Fox - 1985 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Monday, April 06, 2009 4:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology I hate to bring up histological technique for a change of pace but........... Could we get some consensus of opinion as to maximum, minimum and optimal fixation times for different tissues? This is assuming that tissues will be fixed in buffered formalin at room temperature and processed to wax with a standard technique in a processor. This would also require a standard thickness for each tissue type. If there are students out there looking for projects this might seem to be suitable, as a few tissues only could be examined at one time. I know that several papers have been published about fixation in formalin but can't bring to mind any that deal with this aspect of the topic. If there are any students out there who would like a summary of fixation in general I will be happy to email it to them. Barry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SSCALISE <@t> beaumonthospitals.com Mon Apr 6 18:27:35 2009 From: SSCALISE <@t> beaumonthospitals.com (Sharon Scalise) Date: Mon Apr 6 18:27:50 2009 Subject: [Histonet] Autopsy brain processing In-Reply-To: <75A0543E23D3A7458012D9E02EDBEC0002E806CFED@UTHCMS1.uthouston.edu> References: <75A0543E23D3A7458012D9E02EDBEC0002E806CFED@UTHCMS1.uthouston.edu> Message-ID: <49DA57A7.1CA9.00C8.0@beaumonthospitals.com> We are looking to automate our autopsy brain processing (we currently process them manually). I would appreciate any input; you can reply to my direct e-mail address. Thanks. Sharon E. Scalise, HTL (ASCP) Histology Supervisor William Beaumont Hospital Royal Oak, MI 48073 248 898-5981 From raestask <@t> grics.net Mon Apr 6 18:34:10 2009 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Mon Apr 6 18:34:23 2009 Subject: [Histonet] FW: Commercial Source for Direct Immunofluorescence Control Tissue Message-ID: Forwarding this for someone else. Rae Staskiewicz _____ From: Dana Spears [mailto:dspears@mmci.org] Sent: Monday, April 06, 2009 3:11 PM To: histonet-request@lists.utsouthwestern.edu Subject: Commercial Source for Direct Immunofluorescence Control Tissue Anyone know of a commercial source for FITC Control tissue? I've searched the archives and saw the question asked before, but no answer! Thanks to all who respond! Dana Spears, HTL(ASCP) Laboratory Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) (309) 279-3768 (fax) dspears@mmci.org NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. From brian.chelack <@t> pds.usask.ca Mon Apr 6 18:45:16 2009 From: brian.chelack <@t> pds.usask.ca (Brian Chelack) Date: Mon Apr 6 18:45:21 2009 Subject: [Histonet] I'm outta here......me too Message-ID: <4A7078ED1B894AA58189478729872D5E@usask.ca> I read Sally's message a couple of times and I've come to realize that she's right, it's time to go. Recently, the list has been directed by a few persons with outsized egos who are convinced that they (and only they), have all the right answers. I don't think I need to name "names" here, all of us have seen examples of their messages. Woe to those that have ideas that are at odds with this group. Increasingly the responses of these few are adversarial and rude if not down right threatening. Up to now I have stayed with Histonet because I thought that it offered enough good material that I should overlook the excesses of the few. What I realized today was that my staying provides implicit approval of the bullying tactics that have come to be the accepted standard at Histonet. Sorry, but in my view, the information is just not worth it any more. Brian Chelack Special Projects Manager Prairie Diagnostic Services Inc. 52 Campus Drive, Saskatoon, SK S7N 5B4 Phone (306) 966-7211 Fax (306) 966-7244 Email brian.chelack@pds.usask.ca Confidentiality Notice: The information contained in this message and/or attachments may contain confidential and/or privileged information that is intended for the persons or entities addressed above. Any disclosure, copying, retransmission or dissemination is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy the message. From brian.chelack <@t> pds.usask.ca Mon Apr 6 18:45:39 2009 From: brian.chelack <@t> pds.usask.ca (Brian Chelack) Date: Mon Apr 6 18:45:44 2009 Subject: [Histonet] unsubscribe Message-ID: <89A37F40DB4D4C3B922DD9FE91F1E39F@usask.ca> unsubscribe Brian Chelack Special Projects Manager Prairie Diagnostic Services Inc. 52 Campus Drive, Saskatoon, SK S7N 5B4 Phone (306) 966-7211 Fax (306) 966-7244 Email brian.chelack@pds.usask.ca Confidentiality Notice: The information contained in this message and/or attachments may contain confidential and/or privileged information that is intended for the persons or entities addressed above. Any disclosure, copying, retransmission or dissemination is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy the message. From ynwang <@t> u.washington.edu Mon Apr 6 19:04:54 2009 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Mon Apr 6 19:05:00 2009 Subject: [Histonet] Whole human feet Message-ID: <33b7cf2b0904061704g541684bdtd21d4dcd76469661@mail.gmail.com> Hello all, If possible, we would like to obtain histological sections of adult human feet (plane of the anterior-posterior surface). Does anyone know of labs/groups that have done this or something similar? Thank you for your help Yak-Nam Wang From ynwang <@t> u.washington.edu Mon Apr 6 19:16:29 2009 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Mon Apr 6 19:16:38 2009 Subject: [Histonet] Whole human feet In-Reply-To: <33b7cf2b0904061704g541684bdtd21d4dcd76469661@mail.gmail.com> Message-ID: Correction, we would like the cut to be dorsal to plantar surface (e.g. through the metatarsals), not anterior to posterior (that would be a rather large section!) Cheers! Yak-Nam On 4/6/09 5:04 PM, "Yak-Nam Wang" wrote: > Hello all, > > If possible, we would like to obtain histological sections of adult human > feet (plane of the anterior-posterior surface). Does anyone know of > labs/groups that have done this or something similar? > > Thank you for your help > Yak-Nam Wang > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Mon Apr 6 20:37:50 2009 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Mon Apr 6 20:41:31 2009 Subject: [Histonet] Histology Message-ID: <1609291854-1239068484-cardhu_decombobulator_blackberry.rim.net-453420360-@bxe1094.bisx.prod.on.blackberry> Great Barry ... Mouse or human? I think both would be useful to get a poll on. ------Original Message------ From: Rittman, Barry R Sender: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Apr 6, 2009 7:16 PM Subject: [Histonet] Histology I hate to bring up histological technique for a change of pace but........... Could we get some consensus of opinion as to maximum, minimum and optimal fixation times for different tissues? This is assuming that tissues will be fixed in buffered formalin at room temperature and processed to wax with a standard technique in a processor. This would also require a standard thickness for each tissue type. If there are students out there looking for projects this might seem to be suitable, as a few tissues only could be examined at one time. I know that several papers have been published about fixation in formalin but can't bring to mind any that deal with this aspect of the topic. If there are any students out there who would like a summary of fixation in general I will be happy to email it to them. Barry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Tue Apr 7 00:12:05 2009 From: tkngflght <@t> yahoo.com (Cheryl R. Kerry) Date: Tue Apr 7 00:12:09 2009 Subject: [Histonet] email address In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83116@EMAIL.archildrens.org> Message-ID: <25DB4F2AD3C6474CB149407DF2A90D5F@CHERYLSLAPTOP> LMargraf@childmed.dallas.tx.us I sent her an email asking for help on the recent inappropriate activity...if she's not already aware a few concerned emails to her would probably help... Cheryl Kerry, HT(ASCP) Full Staff Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, April 06, 2009 3:18 PM To: histonet@pathology.swmed.edu Subject: [Histonet] email address Does anyone have Dr. Margraf or Dr. Hagler's email address?? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Apr 7 02:10:25 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Apr 7 02:10:29 2009 Subject: [Histonet] Histology Message-ID: <86ADE4EB583CE64799A9924684A0FBBF06833E1D@wahtntex2.waht.swest.nhs.uk> I think one of the things that I've learnt by leaving the world of Histology and dabbling (not too well) in the other disciplines is that there is no such thing as exacts. The maximum and minimum times for fixation for tissue depends on the tissue, the species, the temperature, the manufacturer of the fixative and, ah yes... The time of fixation. I think you need to do what the chemists do and control the reaction; fixatives alter proteins and the rate of reaction is dependent that which I've already said. Why concentrate on one variable? Surely the time taken to fix is just one of the variables and you need to control them all. As in all chemical reactions the time taken is dependent on all the others and you need to determine how that fixative works in your Lab, at your ambient temperature, with your manufacturer of fixative on the species of the tissue you are fixing. Multiple blocks of the same tissue, fixed for differing times and processed the way after being fixed with the same fixative; you can't go wrong. If I've been helpful then I apologise (g). Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: 07 April 2009 00:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology I hate to bring up histological technique for a change of pace but........... Could we get some consensus of opinion as to maximum, minimum and optimal fixation times for different tissues? This is assuming that tissues will be fixed in buffered formalin at room temperature and processed to wax with a standard technique in a processor. This would also require a standard thickness for each tissue type. If there are students out there looking for projects this might seem to be suitable, as a few tissues only could be examined at one time. I know that several papers have been published about fixation in formalin but can't bring to mind any that deal with this aspect of the topic. If there are any students out there who would like a summary of fixation in general I will be happy to email it to them. Barry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From S.J.Ainsworth <@t> bsms.ac.uk Tue Apr 7 02:53:29 2009 From: S.J.Ainsworth <@t> bsms.ac.uk (S.J.Ainsworth@bsms.ac.uk) Date: Tue Apr 7 02:54:26 2009 Subject: [Histonet] Unsubscribe Message-ID: <397EBB11B9C649428A1AB64BC16C66AA01CC18C0@EXCHANGE2.university.brighton.ac.uk> Please can you unsubscribe me from this mailing list. As an inexperienced histologist trying to find my feet I no longer feel like I could ask any questions as I'm sure I would get a torrent of abuse back instead of any helpful comments. Sophie Ainsworth Brighton and Sussex Medical School Medical Research Building Falmer East Sussex BN1 9PX Tel: #44 1273 877886 Fax: #44 1273 877884 From yvan_lindekens <@t> yahoo.com Tue Apr 7 04:05:42 2009 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Tue Apr 7 04:05:47 2009 Subject: [Histonet] Large coverslips? Message-ID: <177710.13415.qm@web110211.mail.gq1.yahoo.com> Hi all, I?m looking for some kind of a DIY coverslip or a tape, plastic foil? anything usable to cover some large slides (+/- 4.5cm * 15cm = 1.8inch * 5.9inch) containing botanical and zoological sections mounted in Canada Balsam. Does anyone has a (cheap?) solution for this? It doesn?t need to be pristine optical quality as the slides are primarely intended to be used on an overhead projector in the class room, but the possibility of viewing them under low power (40x - 100x) would be a real advantage. Thanks in advance four your wisdom and knowledge! Yvan. From yvan_lindekens <@t> yahoo.com Tue Apr 7 04:27:39 2009 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Tue Apr 7 04:27:44 2009 Subject: [Histonet] Thank you! Message-ID: <223083.63651.qm@web110214.mail.gq1.yahoo.com> Many thanks to those who responded to my requist for manuals on Medite COT20 and Shandon Histocentre 2: I received copies of both manuals. Also thanks to those few people with hands-on experience with the Fisher Histomatic 166A, who were willing to answer my questions. After some repair/cleaning on electronics and tubing the unit is up and running. I even begin to understand it's somewhat erratic logic. Yvan. From mthomas <@t> littonlab.com Tue Apr 7 06:47:57 2009 From: mthomas <@t> littonlab.com (Marla Thomas) Date: Tue Apr 7 06:48:06 2009 Subject: [Histonet] Staying... Message-ID: <0KHQ0015TBFDE890@ksle966mailxc2.everestkc.net> I am staying on the list-serve. I have learned a lot from people on this list-serve. I have been in the field since 1970 and I too am still learning things. No one has all of the answers, and sometimes there isn't just one answer. I am staying on the list-serve, and if my input can help one person, or 15 different responses can help me, then the list-serve works. There are still people out there asking questions in between the bickering. Marla Thomas, HT(ASCP) Litton Pathology Associates, PC 700 NW Hunter Dr. Blue Springs, MO 64015 Phone: 816-229-6449 Fax:816-874-4400 CONFIDENTIALITY NOTICE This message and any included attachments are from Litton Pathology Associates, P.C. and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 816-229-6449 and ask for the HIPAA/Compliance Coordinator. From jqb7 <@t> cdc.gov Tue Apr 7 07:10:22 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Apr 7 07:10:56 2009 Subject: [Histonet] Staying... In-Reply-To: <0KHQ0015TBFDE890@ksle966mailxc2.everestkc.net> References: <0KHQ0015TBFDE890@ksle966mailxc2.everestkc.net> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF0511@LTA3VS011.ees.hhs.gov> Amen! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marla Thomas Sent: Tuesday, April 07, 2009 7:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staying... I am staying on the list-serve. I have learned a lot from people on this list-serve. I have been in the field since 1970 and I too am still learning things. No one has all of the answers, and sometimes there isn't just one answer. I am staying on the list-serve, and if my input can help one person, or 15 different responses can help me, then the list-serve works. There are still people out there asking questions in between the bickering. Marla Thomas, HT(ASCP) Litton Pathology Associates, PC 700 NW Hunter Dr. Blue Springs, MO 64015 Phone: 816-229-6449 Fax:816-874-4400 CONFIDENTIALITY NOTICE This message and any included attachments are from Litton Pathology Associates, P.C. and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 816-229-6449 and ask for the HIPAA/Compliance Coordinator. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Tue Apr 7 07:38:52 2009 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Tue Apr 7 07:38:58 2009 Subject: [Histonet] "FREEZY" spray Message-ID: <272572.63083.qm@web45111.mail.sp1.yahoo.com> I?have to agree with Jennifer on the subject of using freezing spray in cryostats?containing potentially infectious agents. I have tried to promote the concept of good cryostat hygiene?with everyone?I train. When I started out in practice I was greeted by ?cryostats that have been left full of shavings.?At our?hospital we?pathologists cut our own frozens, and it seemed to be the policy that the shavings were emptied when you could no longer close the door! One day I opened the door of the cryostat and the draft created by opening the door sent a small blizzard up and I literally inhaled shavings. From that moment on I demanded our?trays be emptied and?wiped clean after each use. It is tough to get some pathologists to comply as they think they are above menial cleaning tasks, and prefer there "histoslaves" to do there clean up. I believe these same culprits wait for their mothers to flush for them. Filthy cryostats also risk cross contamination of slides. If you drop your chuck it will come up covered in coconut. If you chunk out a precious piece of tissue in a freshly cleaned cryostat you?may actually see it?be able to re?embed it. If you use freezing sprays in a cryostat snowstorm you will end up with a blizzard and risk not only inhalation but eye contact as well. If you must use?freezing sprays,?I suggest putting a dot on the chuck at 12:00, removing the chuck and spray the thing back to the ice age if you like.?Return it to the chuck holder with the dot in the 12:00 position.??Bring the chuck back a bit so you can start trimming over again without yet hitting the tissue in case there has been slight change in X-Y orientation. When you are done wipe it out and empty the tray. If you think this is over kill I can recommend a few polluted Beach's here in the New York metropolitan area to take your family this summer! Stephen Peters M.D.? Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 Phone and fax 201 847 7600 www.pathologyinnovations.com From petepath <@t> yahoo.com Tue Apr 7 07:40:59 2009 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Tue Apr 7 07:41:04 2009 Subject: [Histonet] Fw: "FREEZY" spray Message-ID: <699511.33663.qm@web45109.mail.sp1.yahoo.com> ? I?have to agree with Jennifer on the subject of using freezing spray in cryostats?containing potentially infectious agents. I have?tried to promote the concept of good cryostat hygiene?with everyone?I train. When I started out in practice I was greeted?by ?cryostats that have been left full of shavings.?At our?hospital we?pathologists cut our own frozens, and it seemed to be the ?policy that the shavings were emptied when you could no longer close the door! One day I opened the door of the cryostat and the draft created by opening the door sent a small blizzard up and I literally inhaled shavings. From that moment on I demanded our?trays be emptied and?wiped clean after each use. It is tough to get some pathologists to comply as they think they ?are above menial cleaning tasks, and prefer there "histoslaves" to do there clean up. I believe these same culprits wait for their mothers to flush for them. Filthy cryostats also risk cross contamination of slides. If you drop your chuck it will come up covered in coconut. If you chunk out a precious piece of tissue in a freshly cleaned cryostat you?may actually see it?be able t o re?embed it. If you use freezing sprays in a cryostat snowstorm you will end up with a blizzard and risk not only inhalation but eye contact as well. If you must use?freezing sprays,?I suggest putting a dot on the chuck at 12:00, removing the chuck and spray the thing back to the ice age if you like.?Return it to the chuck holder with the dot in the 12:00 position.??Bring the chuck back a bit so you can start trimming over again without yet hitting the tissue in case there has been slight change in X-Y orientation. When you ?are done wipe it out and empty the tray. If you think this is over kill I can recommend a few polluted Beach's here in the New York metropolitan area to take your family this summer! Stephen Peters M.D.? Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 Phone and fax 201 847 7600 www.pathologyinnovations.com From MAGODLEY <@t> aol.com Mon Apr 6 19:10:56 2009 From: MAGODLEY <@t> aol.com (MAGODLEY@aol.com) Date: Tue Apr 7 07:59:36 2009 Subject: [Histonet] gi microwave processing Message-ID: I am starting a gi lab and using a microwave processor for my first time. Any suggestions? SHUR Wave processor. Thanks, Nancy **************A Good Credit Score is 700 or Above. See yours in just 2 easy steps! (http://pr.atwola.com/promoclk/100126575x1221621488x1201450096/aol?redir=http:%2F%2Fwww.freecreditreport.com%2Fpm%2Fdefault.aspx%3Fsc%3D668072%26hmpgID %3D62%26bcd%3DAprilfooterNO62) From crochieresteve <@t> aol.com Tue Apr 7 08:01:18 2009 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Tue Apr 7 08:01:40 2009 Subject: [Histonet] HT openings Message-ID: <8CB859813A164F8-908-6D6@webmailbeta-m08.sysops.aol.com> I have 2 full time openings in my lab at this time. One for a supervisory level HT with IHC experience and one for a becnch level tech. Days, no weekends. If interested please call: Steve Crochiere,HT(ASCP) Histology Supervisor LifePath Partners, LLC @ Mercy Medical Center Springfield, MA 01104 413-748-9543 From Masterson_John <@t> Allergan.com Tue Apr 7 08:37:21 2009 From: Masterson_John <@t> Allergan.com (Masterson_John) Date: Tue Apr 7 08:38:37 2009 Subject: [Histonet] Please unsubscribe me Message-ID: <0C58C4F16F0B67448318A38041CADE4B02991E0A@IRMAIL133.irvine.allergan.com>

This e-mail, including any attachments, is meant only for the intended recipient and may be a confidential communication or a communication privileged by law. If you received this e-mail in error, any review, use, dissemination, distribution, or copying of this e-mail is strictly prohibited. Please notify the sender immediately of the error by return e-mail and please delete this message from your system. Thank you in advance for your cooperation.

From rjbuesa <@t> yahoo.com Tue Apr 7 08:39:19 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 7 08:39:24 2009 Subject: [Histonet] Large coverslips? In-Reply-To: <177710.13415.qm@web110211.mail.gq1.yahoo.com> Message-ID: <81522.20033.qm@web65711.mail.ac4.yahoo.com> Yvan: You could use a transparent thin polystyrene sheet cut to the size you need and cement it to your slides with the Canada balsam. I just got somewhat confused with what you wrote about "low power 40x - 100x". If you are referring to an objective magnification, a 40x is always considered a "high dry power" and a 100x is always an oil immersion (unless it is a "dry" mineralogical high power objective). Any way, a polystyrene sheet will allow you to use the 40x objective. Ren? J. ? --- On Tue, 4/7/09, yvan lindekens wrote: From: yvan lindekens Subject: [Histonet] Large coverslips? To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 5:05 AM Hi all, I?m looking for some kind of a DIY coverslip or a tape, plastic foil? anything usable to cover some large slides (+/- 4.5cm * 15cm = 1.8inch * 5.9inch) containing botanical and zoological sections mounted in Canada Balsam. Does anyone has a (cheap?) solution for this? It doesn?t need to be pristine optical quality as the slides are primarely intended to be used on an overhead projector in the class room, but the possibility of viewing them under low power (40x - 100x) would be a real advantage. Thanks in advance four your wisdom and knowledge! Yvan. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From making <@t> ufl.edu Tue Apr 7 08:43:08 2009 From: making <@t> ufl.edu (MKing) Date: Tue Apr 7 08:43:15 2009 Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 14 In-Reply-To: <200904062309.n36N9lYQ009723@smtp.ufl.edu> References: <200904062309.n36N9lYQ009723@smtp.ufl.edu> Message-ID: <49DB586C.9000608@ufl.edu> a motion is hereby offered to have the active members of histonet vote this troll off the island, or at least request that the list moderator do so. this person has proven to have absolutely nothing of value to contribute to this group, and is only intent on disrupting it. second? ----------------- Message: 16 Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) From: Bernie Taupin Subject: Re: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Message-ID: <453473.65848.qm@web43516.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 > > Instead of spitting your venom try to learn Sort of like you might consider learning correct English? I mean, its one thing if its not your first language, but despite how many people on here called you kindly and helpful, I find it perplexing that you feel compelled to call me a "piece of ignorant", and TWICE, no less. I do not speak French. This list is not in French. Ergo, my awareness (or lack thereof) of proper French diction has absolutely nothing at all to do with anything... aside form providing you a vehicle to call me names and lash out. If you have something to say, say it. The simple fact that you do not like me does not give you- or anyone else- wholesale right to insult me or call me names. You should be ashamed of yourself, Rene. I never expected such antisocial behavior from you, and frankly, when you behave so regrettably, I feel no compulsion to "learn" anything form you! From thaas <@t> mhhcc.org Tue Apr 7 08:47:09 2009 From: thaas <@t> mhhcc.org (Tina Haas/mhhcc.org) Date: Tue Apr 7 08:44:42 2009 Subject: [Histonet] please unsubscribe Message-ID: please unsubscribe This e-mail is for the use of the intended recipient(s) only. The information contained in this communication may be confidential, privileged, or protected by copyright, and may be subject to confidentiality agreements. If you are the intended recipient and you do not wish to receive similar electronic messages from us in future then please respond to the sender to this effect. If you have received this email in error, please notify the sender immediately and then delete it. If you are not the intended recipient, you must not keep, use, disclose, copy or distribute this email without the author's prior permission. From vazquezr <@t> ohsu.edu Tue Apr 7 08:44:45 2009 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Apr 7 08:44:55 2009 Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 14 In-Reply-To: <49DB586C.9000608@ufl.edu> References: <200904062309.n36N9lYQ009723@smtp.ufl.edu> <49DB586C.9000608@ufl.edu> Message-ID: <2A582E8156B45F468A62D1F1D20AF0837144D9@EX-BE08.ohsu.edu> I second it!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MKing Sent: Tuesday, April 07, 2009 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 14 a motion is hereby offered to have the active members of histonet vote this troll off the island, or at least request that the list moderator do so. this person has proven to have absolutely nothing of value to contribute to this group, and is only intent on disrupting it. second? ----------------- Message: 16 Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) From: Bernie Taupin Subject: Re: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Message-ID: <453473.65848.qm@web43516.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 > > Instead of spitting your venom try to learn Sort of like you might consider learning correct English? I mean, its one thing if its not your first language, but despite how many people on here called you kindly and helpful, I find it perplexing that you feel compelled to call me a "piece of ignorant", and TWICE, no less. I do not speak French. This list is not in French. Ergo, my awareness (or lack thereof) of proper French diction has absolutely nothing at all to do with anything... aside form providing you a vehicle to call me names and lash out. If you have something to say, say it. The simple fact that you do not like me does not give you- or anyone else- wholesale right to insult me or call me names. You should be ashamed of yourself, Rene. I never expected such antisocial behavior from you, and frankly, when you behave so regrettably, I feel no compulsion to "learn" anything form you! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Apr 7 08:50:14 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Apr 7 08:50:21 2009 Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 14 Message-ID: <86ADE4EB583CE64799A9924684A0FBBF06833EEC@wahtntex2.waht.swest.nhs.uk> Can I just say that you are giving him more importance than he's worth and you are perpetuating it? Obviously he must feed off your responses so stop being manipulated; just ignore or delete or make an Outlook rule, as I have, that his e-mails go to trash!! Bernie, Bernie, where are you? I can't see you. Well I can cos people him replying to you and the craps at the bottom of the e-mail..... My point so stop inflicting me on him, please!!!!! Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 07 April 2009 14:45 To: MKing; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Histonet Digest, Vol 65, Issue 14 I second it!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MKing Sent: Tuesday, April 07, 2009 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 14 a motion is hereby offered to have the active members of histonet vote this troll off the island, or at least request that the list moderator do so. this person has proven to have absolutely nothing of value to contribute to this group, and is only intent on disrupting it. second? ----------------- Message: 16 Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) From: Bernie Taupin Subject: Re: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Message-ID: <453473.65848.qm@web43516.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 > > Instead of spitting your venom try to learn Sort of like you might consider learning correct English? I mean, its one thing if its not your first language, but despite how many people on here called you kindly and helpful, I find it perplexing that you feel compelled to call me a "piece of ignorant", and TWICE, no less. I do not speak French. This list is not in French. Ergo, my awareness (or lack thereof) of proper French diction has absolutely nothing at all to do with anything... aside form providing you a vehicle to call me names and lash out. If you have something to say, say it. The simple fact that you do not like me does not give you- or anyone else- wholesale right to insult me or call me names. You should be ashamed of yourself, Rene. I never expected such antisocial behavior from you, and frankly, when you behave so regrettably, I feel no compulsion to "learn" anything form you! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From khicks71 <@t> comcast.net Tue Apr 7 09:00:10 2009 From: khicks71 <@t> comcast.net (khicks71@comcast.net) Date: Tue Apr 7 09:00:13 2009 Subject: [Histonet] Pricing of blocks Message-ID: <1789926878.1562381239112810821.JavaMail.root@sz0061a.emeryville.ca.mail.comcast.net> Good Morning everyone! I am opening a Histology Lab in our Dermatology practice, we routinely do approx. 10,000 biopsies a year.? I would love some input from anyone out there that can assisit me with obtaining a fair price for this task.? The doctors are going to be paying me per block.? I will be grossing in, processing, cutting &?staining, maintaining CLIA, and of course maintaining quality. Thanks so much. Kathy Hicks H.T.(ASCP) DPNS Surgical Center 400 Skokie Blvd. Suite 450 Northbrook, Illinois? 60062 From michelle.broome <@t> novartis.com Tue Apr 7 09:00:45 2009 From: michelle.broome <@t> novartis.com (michelle.broome@novartis.com) Date: Tue Apr 7 09:00:56 2009 Subject: [Histonet] Job opportunity in Cambridge, MA In-Reply-To: <200904071346.n37DkcgE013564@ch1ssaenov01.novartis.com> Message-ID: Hello all! We are a small pathology group within a large, Cambridge, MA based organization looking for a histologist. Our main function is to support drug development in a preclinical setting. The work would involve basic histology, IHC, and special staining on rodent tissue. Our ideal candidate is self-motivated, able to work independently but also as a team player, enthusiastic, and able to multitask. If that sounds like you, please visit our job posting at: http://www.novartis.com/careers/job-search/brassring/index.shtml and search for Job ID: 48106BR. We hope to speak with you soon! Regards, Michelle Broome Team Leader Preclinical Safety, Pathology Translational Sciences Novartis Institutes for BioMedical Research, Inc. 250 Massachusetts Avenue Cambridge, MA 02139 USA Email : michelle.broome@novartis.com From barbara.schormair <@t> helmholtz-muenchen.de Tue Apr 7 09:05:19 2009 From: barbara.schormair <@t> helmholtz-muenchen.de (Barbara Schormair) Date: Tue Apr 7 09:05:25 2009 Subject: [Histonet] Paraffin embedded mouse embryos - too dry Message-ID: <49DB5D9F.5030009@helmholtz-muenchen.de> Dear all, I'm having trouble with my paraffin embedded mouse embryos. We use E12.5 to E15.5 embryos and we embed and cut the whole embryo. When we try to cut, the paraffin sheets are fine, but as soon as we get to the embryo, the tissue disrupts and tears. I also hear rasping or scraping sounds when cutting throught the embryo, so the problem seems to be that the tissue is too dry or too hard. Here is my current protocol: Dissect embryos from the uterus and amnion in 1x PBS, then fix overnight at 4?C in 4% PFA. Transfer to 70% Ethanol and store at 4?C. After 2 days to several weeks, we continue with the processing as follows: E12.5: 96% Ethanol for 15min, 100% Ethanol for 15min, Xylol for 15min (I also tried 10min Xylol and 8min Xylol, but with the same results). E15.5: 96% Ethanol for 30min, 100% Ethanol for 30min, Xylol for 30min (I also tried 20min Xylol and 15min Xylol, but with the same results). Then bring into Paraffin and leave overnight at 65?C, next day embedding and cooling down on-5?C cooling plate. Store at 4?C. What is the problem? Storage in 70% Ethanol for too long? Xylol incubation times too long or too short? What is the critical step - the ethanol incubation or the xylol incubation - should I try different times in Xylol or also try and change the Ethanol incubation times? Thanks in advance for any help! Best regards, Barbara -- Dipl. Biol. Barbara Schormair PhD student Institute of Human Genetics Tel.: 0049-89-3187-3953 Fax: 0049-89-3187-3297 e-Mail: barbara.schormair@helmholtz-muenchen.de Helmholtz Zentrum M?nchen German Research Center for Environmental Health (GmbH) Ingolstaedter Landstra?e 1 D-85764 Neuherberg Germany Chairman of Supervisory Board: MinDir Dr. Peter Lange Board of Directors: Prof. Dr. G?nther Wess and Dr. Nikolaus Blum Register of Societies: Amtsgericht M?nchen HRB 6466 From joost.bruijntjes <@t> tno.nl Tue Apr 7 09:13:44 2009 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Tue Apr 7 09:13:53 2009 Subject: [Histonet] Eosinophilic granulocytes Message-ID: <8865601DD17A554CB489C17FFD8A51B20242440C@MAIL04.tsn.tno.nl> Hi Is anyone of you aware of a marker/method to stain eosinophilic granulocytes in mouse lung tissue which is formalin fixed and paraffin embedded? Thanks Joost Bruijntjes TNO Quality of Life Zeist Holland TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From llewllew <@t> shaw.ca Tue Apr 7 09:11:46 2009 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Apr 7 09:13:58 2009 Subject: [Histonet] Large coverslips? In-Reply-To: <177710.13415.qm@web110211.mail.gq1.yahoo.com> References: <177710.13415.qm@web110211.mail.gq1.yahoo.com> Message-ID: <84174F5D9EBD4F01B181116A23E7E2D1@BryanPC> Whenever I needed to prepare projection slides I coverslipped with another regular glass slide, then cleaned up the edges after the mounting medium dried. Low power microscopy was still possible providing the working distance for the objective lens was greater than 1 mm. Bryan Llewellyn ----- Original Message ----- From: "yvan lindekens" To: Sent: Tuesday, April 07, 2009 2:05 AM Subject: [Histonet] Large coverslips? Hi all, I?m looking for some kind of a DIY coverslip or a tape, plastic foil? anything usable to cover some large slides (+/- 4.5cm * 15cm = 1.8inch * 5.9inch) containing botanical and zoological sections mounted in Canada Balsam. Does anyone has a (cheap?) solution for this? It doesn?t need to be pristine optical quality as the slides are primarely intended to be used on an overhead projector in the class room, but the possibility of viewing them under low power (40x - 100x) would be a real advantage. Thanks in advance four your wisdom and knowledge! Yvan. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Apr 7 09:16:28 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Apr 7 09:16:32 2009 Subject: [Histonet] Job opportunity in Cambridge, MA Message-ID: <86ADE4EB583CE64799A9924684A0FBBF06833EFB@wahtntex2.waht.swest.nhs.uk> Try: Apply this rule after message arrives with Bernie Taulin in the senders address Delete it Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of michelle.broome@novartis.com Sent: 07 April 2009 15:01 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Job opportunity in Cambridge, MA Hello all! We are a small pathology group within a large, Cambridge, MA based organization looking for a histologist. Our main function is to support drug development in a preclinical setting. The work would involve basic histology, IHC, and special staining on rodent tissue. Our ideal candidate is self-motivated, able to work independently but also as a team player, enthusiastic, and able to multitask. If that sounds like you, please visit our job posting at: http://www.novartis.com/careers/job-search/brassring/index.shtml and search for Job ID: 48106BR. We hope to speak with you soon! Regards, Michelle Broome Team Leader Preclinical Safety, Pathology Translational Sciences Novartis Institutes for BioMedical Research, Inc. 250 Massachusetts Avenue Cambridge, MA 02139 USA Email : michelle.broome@novartis.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Apr 7 09:23:30 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Apr 7 09:23:35 2009 Subject: [Histonet] Getting rid of pests Message-ID: <86ADE4EB583CE64799A9924684A0FBBF06833F02@wahtntex2.waht.swest.nhs.uk> Or Bernie Taupin....... Even. Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: 07 April 2009 15:16 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Job opportunity in Cambridge, MA Try: Apply this rule after message arrives with Bernie Taulin in the senders address Delete it Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of michelle.broome@novartis.com Sent: 07 April 2009 15:01 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Job opportunity in Cambridge, MA Hello all! We are a small pathology group within a large, Cambridge, MA based organization looking for a histologist. Our main function is to support drug development in a preclinical setting. The work would involve basic histology, IHC, and special staining on rodent tissue. Our ideal candidate is self-motivated, able to work independently but also as a team player, enthusiastic, and able to multitask. If that sounds like you, please visit our job posting at: http://www.novartis.com/careers/job-search/brassring/index.shtml and search for Job ID: 48106BR. We hope to speak with you soon! Regards, Michelle Broome Team Leader Preclinical Safety, Pathology Translational Sciences Novartis Institutes for BioMedical Research, Inc. 250 Massachusetts Avenue Cambridge, MA 02139 USA Email : michelle.broome@novartis.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Tue Apr 7 09:23:39 2009 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Apr 7 09:23:45 2009 Subject: [Histonet] FW: Cervical cancer mission in Peru Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4367D3A9A79D@HPEMX3.HealthPartners.int> ________________________________ From: Savaloja, Lynnette C Sent: Thursday, April 02, 2009 11:06 AM To: Webb, Dorothy L Cc: Semerad, Shelly A Subject: FW: Cervical cancer mission in Peru Importance: High Hey Dorothy, Got an old microtome laying around (see below)? Let me know... L ;) ________________________________ From: Fischer, Andrew [mailto:Andrew.Fischer@umassmemorial.org] Sent: Thursday, April 02, 2009 10:58 AM To: members@lists.cytopathology.org Subject: [ASC Listserv] Cervical cancer mission in Peru Dear ASC members, Does anyone have a working microtome that could be donated to the INCCA (International Cervical Cancer Foundation) cervical cancer screening and treatment mission in Peru? This important program does not yet have a microtome to allow evaluation of the biopsies/cones. We will need it by the end of May. Please contact me if you can help. For more information on the Peru mission, see http://www.theincca.org/indextest2.html Sincerely, Andy Fischer UMASS The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, transmission, re-transmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and delete the material from any computer. ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 From billodonnell <@t> catholichealth.net Tue Apr 7 09:32:03 2009 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Apr 7 09:32:17 2009 Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 14 In-Reply-To: <49DB586C.9000608@ufl.edu> References: <200904062309.n36N9lYQ009723@smtp.ufl.edu> <49DB586C.9000608@ufl.edu> Message-ID: I have tried for the past few days to stay out of this. Might I suggest that if there is a person or persons causing anyone discomfort or problems, simply add them to your blocked list in you e-mail program. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MKing Sent: Tuesday, April 07, 2009 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 14 a motion is hereby offered to have the active members of histonet vote this troll off the island, or at least request that the list moderator do so. this person has proven to have absolutely nothing of value to contribute to this group, and is only intent on disrupting it. second? ----------------- Message: 16 Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) From: Bernie Taupin Subject: Re: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Message-ID: <453473.65848.qm@web43516.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 > > Instead of spitting your venom try to learn Sort of like you might consider learning correct English? I mean, its one thing if its not your first language, but despite how many people on here called you kindly and helpful, I find it perplexing that you feel compelled to call me a "piece of ignorant", and TWICE, no less. I do not speak French. This list is not in French. Ergo, my awareness (or lack thereof) of proper French diction has absolutely nothing at all to do with anything... aside form providing you a vehicle to call me names and lash out. If you have something to say, say it. The simple fact that you do not like me does not give you- or anyone else- wholesale right to insult me or call me names. You should be ashamed of yourself, Rene. I never expected such antisocial behavior from you, and frankly, when you behave so regrettably, I feel no compulsion to "learn" anything form you! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pritchm <@t> ccf.org Tue Apr 7 09:32:13 2009 From: pritchm <@t> ccf.org (Pritchard, Michele) Date: Tue Apr 7 09:35:01 2009 Subject: [Histonet] Eosinophilic granulocytes In-Reply-To: <8865601DD17A554CB489C17FFD8A51B20242440C@MAIL04.tsn.tno.nl> Message-ID: <7CEB62F1535B9E44AD8A5FEFB2E10F6E0215143F@CCHSCLEXMB56.cc.ad.cchs.net> Good morning: I have good luck by simply using haematoxylin and eosin staining....the eosinophils pop right out by virtue of their bright pink/red granules! When used in conjunction with nuclear morphology, one can, with confidence, call these cells eosinophils. I have never performed IHC for eosinophils, but just did a quick search and found this helpful website. I am sure there are more websites like this, but this one will certainly get you started. http://www.antibodybeyond.com/reviews/cell-markers/eosinophil-marker.htm Best of luck -->mtp Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: pritchm@ccf.org Lab location: Lerner Research Institute NE4-214 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruijntjes, J.P. (Joost) Sent: Tuesday, April 07, 2009 10:14 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosinophilic granulocytes Hi Is anyone of you aware of a marker/method to stain eosinophilic granulocytes in mouse lung tissue which is formalin fixed and paraffin embedded? Thanks Joost Bruijntjes TNO Quality of Life Zeist Holland TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================================== P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From algranth <@t> email.arizona.edu Tue Apr 7 09:39:59 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Tue Apr 7 09:40:05 2009 Subject: [Histonet] Paraffin embedded mouse embryos - too dry In-Reply-To: <49DB5D9F.5030009@helmholtz-muenchen.de> References: <49DB5D9F.5030009@helmholtz-muenchen.de> Message-ID: <4F399656-FA17-4157-BE2F-1B7A7AA597D0@email.arizona.edu> I sometimes get embryos in the lab for processing and here are the protocols that I use for embryos in these stages: E12.5 - 70% Ethanol - 12 min 95% Ethanol - 12 min 100% Ethanol x 2 - 12 min each Xylene x 2 - 12 min each Paraffin - 30 min Paraffin x 2 - 1 hr each no vacuum or pressure used except for the last paraffin E15.5 - 70% Ethanol - 2 hrs (or two changes for an hour each) 95% Ethanol - 1 hr 95% Ethanol - 2 hrs 100% Ethanol x 3 - 1 hr. eash Xylene x 2 - 2 hrs each Paraffin x 4 - total of 10 hrs I use some vacuum and pressure - in the last Xylene and in the paraffins These protocols were provided to my by Gayle Callis and I have modified them a little. They work great for me and I don't have any problems with the sectioning. Good luck! Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello On Apr 7, 2009, at 7:05 AM, Barbara Schormair wrote: > Dear all, > > I'm having trouble with my paraffin embedded mouse embryos. We use > E12.5 to E15.5 embryos and we embed and cut the whole embryo. When > we try to cut, the paraffin sheets are fine, but as soon as we get > to the embryo, the tissue disrupts and tears. I also hear rasping or > scraping sounds when cutting throught the embryo, so the problem > seems to be that the tissue is too dry or too hard. > Here is my current protocol: > Dissect embryos from the uterus and amnion in 1x PBS, then fix > overnight at 4?C in 4% PFA. Transfer to 70% Ethanol and store at 4?C. > After 2 days to several weeks, we continue with the processing as > follows: > E12.5: 96% Ethanol for 15min, 100% Ethanol for 15min, Xylol for > 15min (I also tried 10min Xylol and 8min Xylol, but with the same > results). > E15.5: 96% Ethanol for 30min, 100% Ethanol for 30min, Xylol for > 30min (I also tried 20min Xylol and 15min Xylol, but with the same > results). > Then bring into Paraffin and leave overnight at 65?C, next day > embedding and cooling down on-5?C cooling plate. Store at 4?C. > > What is the problem? Storage in 70% Ethanol for too long? Xylol > incubation times too long or too short? > What is the critical step - the ethanol incubation or the xylol > incubation - should I try different times in Xylol or also try and > change the Ethanol incubation times? > > Thanks in advance for any help! > > Best regards, > > Barbara > > > > -- > Dipl. Biol. Barbara Schormair > PhD student Institute of Human Genetics > Tel.: 0049-89-3187-3953 > Fax: 0049-89-3187-3297 > e-Mail: barbara.schormair@helmholtz-muenchen.de > > Helmholtz Zentrum M?nchen > German Research Center for Environmental Health (GmbH) > Ingolstaedter Landstra?e 1 > D-85764 Neuherberg > Germany > > > Chairman of Supervisory Board: MinDir Dr. Peter Lange > Board of Directors: Prof. Dr. G?nther Wess and Dr. Nikolaus Blum > Register of Societies: Amtsgericht M?nchen HRB 6466 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PMonfils <@t> Lifespan.org Tue Apr 7 09:44:59 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Apr 7 09:45:22 2009 Subject: [Histonet] Entamoeba Trophozoites In-Reply-To: <49D626AE020000EE00027F0A@smtp-gw.hurleymc.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CAD@LSRIEXCH1.lsmaster.lifespan.org> I haven't checked my email for a few days! Yikes, over 150 messages! But I'll offer this anyway, however late. Entamoeba trophs can easily be demonstrated either in dried fixed smears or in paraffin sections by PAS. Their cytoplasm is loaded with glycogen so they stain very dark. From PMonfils <@t> Lifespan.org Tue Apr 7 09:48:04 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Apr 7 09:48:31 2009 Subject: [Histonet] When sectioning small long bones in PMMA .... Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CAE@LSRIEXCH1.lsmaster.lifespan.org> ... such as a mouse femur, do you prefer to orient the bone parallel to the knife edge or perpendicular to the knife edge (or diagonal)? From nefff <@t> staff.uni-marburg.de Tue Apr 7 09:57:12 2009 From: nefff <@t> staff.uni-marburg.de (Dr. Frauke Neff) Date: Tue Apr 7 09:57:22 2009 Subject: [Histonet] cryoprotection possible-thanks! Message-ID: <20090407165712.z5uv933xcg4gcgkk@home.staff.uni-marburg.de> Dear Histonetters, I just wanted to say a big "thank you" to all who responded to my cryoprotection request. We managed it to fix the samples with GA and do a sucrose step accourding to your suggestions: it worked! happy eastern to all, Frauke From algranth <@t> email.arizona.edu Tue Apr 7 09:59:14 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Tue Apr 7 09:59:22 2009 Subject: [Histonet] When sectioning small long bones in PMMA .... In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835CAE@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E03835CAE@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <394EE124-15C5-48F0-B5E9-808C4D57E90D@email.arizona.edu> neither - I like it on the diagonal. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello On Apr 7, 2009, at 7:48 AM, Monfils, Paul wrote: > ... such as a mouse femur, do you prefer to orient the bone parallel > to the knife edge or perpendicular to the knife edge (or diagonal)? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ratliffjack <@t> hotmail.com Tue Apr 7 10:00:15 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Apr 7 10:00:27 2009 Subject: [Histonet] When sectioning small long bones in PMMA .... In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835CAE@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E03835CAE@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: I have always orientated the specimen in an AP view and taken longitudinal/frontal sections. As for parallel or perpendicular, I may not be completely sure of what you mean. Jack On Apr 7, 2009, at 9:48 AM, "Monfils, Paul" wrote: > ... such as a mouse femur, do you prefer to orient the bone parallel > to the knife edge or perpendicular to the knife edge (or diagonal)? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PMonfils <@t> Lifespan.org Tue Apr 7 09:58:41 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Apr 7 10:00:57 2009 Subject: [Histonet] Removing PMMA blocks from glass vials - feedback Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CAF@LSRIEXCH1.lsmaster.lifespan.org> Thanks to all who offered advice on this topic. I followed the advice given but my experience was a bit different from that which was described, so I thought I would share it and see if anyone else has had a similar experience. I placed the vials in the -20 freezer for an hour, then went back intending to take them out, wrap them in an underpad and hit them with a hammer. But the vials had already shattered, apparently with some force since bits of glass were everywhere. Has anyone else experienced this? It may be due to the type of vials I used, scintillation vials, which are made of a glass that seems quite hard and somewhat thin. From cmiller <@t> physlab.com Tue Apr 7 10:30:59 2009 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Apr 7 10:31:03 2009 Subject: [Histonet] Nice one Kemlo In-Reply-To: <58F70F8C715A45D0AB2E64C806B81D43@merlin> References: <58F70F8C715A45D0AB2E64C806B81D43@merlin> Message-ID: I for one would be lost with out this group. I need and miss the collaboration of fellow histotechs on a daily basis. Cheri -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of alan taylor Sent: Saturday, April 04, 2009 11:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nice one Kemlo Nice one Kemlo: /histonet From Vickroy.Jim <@t> mhsil.com Tue Apr 7 10:53:36 2009 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Apr 7 10:53:50 2009 Subject: [Histonet] MAMMOGLOBIN Message-ID: <24A4826E8EF0964D86BC5317306F58A52BB259341F@mmc-mail.ad.mhsil.com> Has anyone used the DAKO predilute antibody, Mammoglobin, on the Ventana Benchmark XT? And if so.....could you share the protocol you used? Finally looking on the Dako website it appears that they have ready to use, could you share with me which antibody should be used with the Ventana I-view detection kit? Thanks Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy.jim@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From keithc <@t> mmc.org Tue Apr 7 11:41:20 2009 From: keithc <@t> mmc.org (Cynthia Keith) Date: Tue Apr 7 11:41:50 2009 Subject: [Histonet] unsubscribe Message-ID: <49DB49F0.C640.000E.0@mmc.org> Cynthia Keith HT(ASCP) Histology Supervisor NorDx 102 Campus drive Scarborough, ME 04074 Tel: [207] 885-7907 Fax:[207] 885-7538 Email:keithc@mmc.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the use of the intended recipient(s) only and may contain information that is privileged, confidential, and prohibited from unauthorized disclosure under applicable law. If you are not the intended recipient of this message, any dissemination, distribution, or copying of this message is strictly prohibited. If you received this message in error, please notify the sender by reply email and destroy all copies of the original message and attachments. From LINDA.MARGRAF <@t> childrens.com Tue Apr 7 11:52:17 2009 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Tue Apr 7 11:52:28 2009 Subject: [Histonet] histonet off-topic comments In-Reply-To: <49DB586C.9000608@ufl.edu> References: <200904062309.n36N9lYQ009723@smtp.ufl.edu> <49DB586C.9000608@ufl.edu> Message-ID: <49DB3DD7.F783.00DA.0@childrens.com> Dear Histonet members: I have removed Bernie Taupin from the list. Let's please get back to the topic of histology and related fields.Thanks Linda M Histonet administrator >>> MKing 4/7/2009 8:43 AM >>> a motion is hereby offered to have the active members of histonet vote this troll off the island, or at least request that the list moderator do so. this person has proven to have absolutely nothing of value to contribute to this group, and is only intent on disrupting it. second? ----------------- Message: 16 Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) From: Bernie Taupin Subject: Re: [Histonet] Nice one Kemlo To: histonet@lists.utsouthwestern.edu Message-ID: <453473.65848.qm@web43516.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 > > Instead of spitting your venom try to learn Sort of like you might consider learning correct English? I mean, its one thing if its not your first language, but despite how many people on here called you kindly and helpful, I find it perplexing that you feel compelled to call me a "piece of ignorant", and TWICE, no less. I do not speak French. This list is not in French. Ergo, my awareness (or lack thereof) of proper French diction has absolutely nothing at all to do with anything... aside form providing you a vehicle to call me names and lash out. If you have something to say, say it. The simple fact that you do not like me does not give you- or anyone else- wholesale right to insult me or call me names. You should be ashamed of yourself, Rene. I never expected such antisocial behavior from you, and frankly, when you behave so regrettably, I feel no compulsion to "learn" anything form you! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please consider the environment before printing this e-mail

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From Tammy.Barnhart <@t> mckennan.org Tue Apr 7 11:53:37 2009 From: Tammy.Barnhart <@t> mckennan.org (Tammy Barnhart) Date: Tue Apr 7 11:53:45 2009 Subject: [Histonet] AE1/AE3 nuclear staining Message-ID: <650B78513FAF9D41B8AE22F991AFCA62024D740D@mck235.averamail.net> We have recently been having dark nuclear staining with our AE1/AE3 antibody. This is a recent event and we cannot figure out what is going on. It started gradually and has been increasing in intensity. We have not changed antibody lots or any other part of our protocol. Has anyone seen this before? Any suggestions on what is happening here? Tammy Barnhart, BS, HTL(ASCP) Avera McKennan Hospital ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From janderson <@t> halozyme.com Tue Apr 7 11:54:11 2009 From: janderson <@t> halozyme.com (Jennifer Anderson) Date: Tue Apr 7 11:54:28 2009 Subject: [Histonet] Paraffin embedded mouse embryos - too dry In-Reply-To: <4F399656-FA17-4157-BE2F-1B7A7AA597D0@email.arizona.edu> References: <49DB5D9F.5030009@helmholtz-muenchen.de> <4F399656-FA17-4157-BE2F-1B7A7AA597D0@email.arizona.edu> Message-ID: Good morning. A colleague of mine recently placed the 10% formalin fixed mouse embryos in 2% agarose, let them solidify, then process at 30 minutes per station under pressure/vacuum. The protocol was published, but right now I don't have that information. Hope this helps in some way! Jen Anderson The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Tuesday, April 07, 2009 7:40 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin embedded mouse embryos - too dry I sometimes get embryos in the lab for processing and here are the protocols that I use for embryos in these stages: E12.5 - 70% Ethanol - 12 min 95% Ethanol - 12 min 100% Ethanol x 2 - 12 min each Xylene x 2 - 12 min each Paraffin - 30 min Paraffin x 2 - 1 hr each no vacuum or pressure used except for the last paraffin E15.5 - 70% Ethanol - 2 hrs (or two changes for an hour each) 95% Ethanol - 1 hr 95% Ethanol - 2 hrs 100% Ethanol x 3 - 1 hr. eash Xylene x 2 - 2 hrs each Paraffin x 4 - total of 10 hrs I use some vacuum and pressure - in the last Xylene and in the paraffins These protocols were provided to my by Gayle Callis and I have modified them a little. They work great for me and I don't have any problems with the sectioning. Good luck! Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello On Apr 7, 2009, at 7:05 AM, Barbara Schormair wrote: > Dear all, > > I'm having trouble with my paraffin embedded mouse embryos. We use > E12.5 to E15.5 embryos and we embed and cut the whole embryo. When > we try to cut, the paraffin sheets are fine, but as soon as we get > to the embryo, the tissue disrupts and tears. I also hear rasping or > scraping sounds when cutting throught the embryo, so the problem > seems to be that the tissue is too dry or too hard. > Here is my current protocol: > Dissect embryos from the uterus and amnion in 1x PBS, then fix > overnight at 4?C in 4% PFA. Transfer to 70% Ethanol and store at 4?C. > After 2 days to several weeks, we continue with the processing as > follows: > E12.5: 96% Ethanol for 15min, 100% Ethanol for 15min, Xylol for > 15min (I also tried 10min Xylol and 8min Xylol, but with the same > results). > E15.5: 96% Ethanol for 30min, 100% Ethanol for 30min, Xylol for > 30min (I also tried 20min Xylol and 15min Xylol, but with the same > results). > Then bring into Paraffin and leave overnight at 65?C, next day > embedding and cooling down on-5?C cooling plate. Store at 4?C. > > What is the problem? Storage in 70% Ethanol for too long? Xylol > incubation times too long or too short? > What is the critical step - the ethanol incubation or the xylol > incubation - should I try different times in Xylol or also try and > change the Ethanol incubation times? > > Thanks in advance for any help! > > Best regards, > > Barbara > > > > -- > Dipl. Biol. Barbara Schormair > PhD student Institute of Human Genetics > Tel.: 0049-89-3187-3953 > Fax: 0049-89-3187-3297 > e-Mail: barbara.schormair@helmholtz-muenchen.de > > Helmholtz Zentrum M?nchen > German Research Center for Environmental Health (GmbH) > Ingolstaedter Landstra?e 1 > D-85764 Neuherberg > Germany > > > Chairman of Supervisory Board: MinDir Dr. Peter Lange > Board of Directors: Prof. Dr. G?nther Wess and Dr. Nikolaus Blum > Register of Societies: Amtsgericht M?nchen HRB 6466 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vapatpxs <@t> yahoo.com Tue Apr 7 12:17:43 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Tue Apr 7 12:17:47 2009 Subject: [Histonet] Large coverslips? Message-ID: <693405.22892.qm@web46103.mail.sp1.yahoo.com> Hi Yvan, Does it have to be a coverslip? There are some mounting media that harden and form a barrier with optical qualities similar to that of glass. It might not be a perfect solution, but it might work. I think the stuff I used was called Crystal Mount (?). Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr.., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Tue, 4/7/09, yvan lindekens wrote: > From: yvan lindekens > Subject: [Histonet] Large coverslips? > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, April 7, 2009, 9:05 AM > > Hi all, > > I?m looking for some kind of a DIY coverslip or a tape, > plastic foil? anything usable to cover some large slides > (+/- 4.5cm * 15cm = 1.8inch * 5.9inch) containing botanical > and zoological sections mounted in Canada Balsam. > > Does anyone has a (cheap?) solution for this? It > doesn?t need to be pristine optical quality as the slides > are primarely intended to be used on an overhead projector > in the class room, but the possibility of viewing them under > low power (40x - 100x) would be a real advantage. > > Thanks in advance four your wisdom and knowledge! > > Yvan. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cathy <@t> wasatchhisto.com Tue Apr 7 12:23:02 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Tue Apr 7 12:23:31 2009 Subject: [Histonet] HI Downdraft fume extractor for sale Message-ID: <38D5EDE70AE14F2B9C3ABF7029193E5A@shop1e2e996aa5> I have a HI downdraft fume extractor for $50 and we will provide carbon to refill the drawer. Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 From cathy <@t> wasatchhisto.com Tue Apr 7 12:28:44 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Tue Apr 7 12:29:05 2009 Subject: [Histonet] breaking glass jar/vials for MMA embedded specimens Message-ID: <173DDB7C55744B8A8E88B90232846319@shop1e2e996aa5> Yes, the scintillation vials are notorious for shattering in the freezer. However, if you put them in a plastic container with a lid on, the shattered glass will be contained and not all over your freezer. When breaking larger glass containers, again place them in the freezer, remove from the freezer after 20 minutes or so, take the lid off, wrap jar in paper towels and hold the ends closed. Wear glovesand eye protection in case shards of glass escape. Strike the jar with a hammer and unroll the paper towel over a garbage can. Rinse the block in tap water to remove any small shards of glass. This is a great way to vent frustration!! Cathy A. Mayton Wasatch Histo Consultants, Inc. From gu.lang <@t> gmx.at Tue Apr 7 12:36:14 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Apr 7 12:36:23 2009 Subject: AW: [Histonet] MAMMOGLOBIN In-Reply-To: <24A4826E8EF0964D86BC5317306F58A52BB259341F@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A52BB259341F@mmc-mail.ad.mhsil.com> Message-ID: I tried also this antibody on Ventana Benchmark. Pity, I had no results. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Vickroy, Jim Gesendet: Dienstag, 07. April 2009 17:54 An: 'Histonet@lists.utsouthwestern.edu' Betreff: [Histonet] MAMMOGLOBIN Has anyone used the DAKO predilute antibody, Mammoglobin, on the Ventana Benchmark XT? And if so.....could you share the protocol you used? Finally looking on the Dako website it appears that they have ready to use, could you share with me which antibody should be used with the Ventana I-view detection kit? Thanks Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy.jim@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Apr 7 14:16:44 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 7 14:16:48 2009 Subject: [Histonet] AE1/AE3 nuclear staining In-Reply-To: <650B78513FAF9D41B8AE22F991AFCA62024D740D@mck235.averamail.net> Message-ID: <937057.4220.qm@web65706.mail.ac4.yahoo.com> Have you changed any of the reagents in your X-press tissue processor or other aspect of your tissue processing protocol? This could be?a factor. Ren? J. --- On Tue, 4/7/09, Tammy Barnhart wrote: From: Tammy Barnhart Subject: [Histonet] AE1/AE3 nuclear staining To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 12:53 PM We have recently been having dark nuclear staining with our AE1/AE3 antibody. This is a recent event and we cannot figure out what is going on. It started gradually and has been increasing in intensity. We have not changed antibody lots or any other part of our protocol. Has anyone seen this before? Any suggestions on what is happening here? Tammy Barnhart, BS, HTL(ASCP) Avera McKennan Hospital ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Tue Apr 7 14:22:13 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Apr 7 14:22:19 2009 Subject: [Histonet] AE1/AE3 nuclear staining In-Reply-To: <650B78513FAF9D41B8AE22F991AFCA62024D740D@mck235.averamail.net> References: <650B78513FAF9D41B8AE22F991AFCA62024D740D@mck235.averamail.net> Message-ID: <5b6eb13e0904071222q37c9449fj3ad723ffb6e57bfe@mail.gmail.com> When was the last time you checked the pH of your retrieval solution? Or are you using an enzyme? Mark Tarango On Tue, Apr 7, 2009 at 9:53 AM, Tammy Barnhart wrote: > We have recently been having dark nuclear staining with our AE1/AE3 > antibody. This is a recent event and we cannot figure out what is going > on. It started gradually and has been increasing in intensity. We have > not changed antibody lots or any other part of our protocol. Has anyone > seen this before? Any suggestions on what is happening here? > > Tammy Barnhart, BS, HTL(ASCP) > > Avera McKennan Hospital > > > > ----------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure, or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gentras <@t> vetmed.auburn.edu Tue Apr 7 14:28:16 2009 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Tue Apr 7 14:28:21 2009 Subject: [Histonet] RE: Tissue Capture Pen Message-ID: <49DBA950.2080301@vetmed.auburn.edu> hello, if any of you are using and/or have used the Ted Pella, Inc. Tissue Capture Pen will you be so kind as to share with me your experience (s), pros, cons and whatever else you deem necessary? Also, does it's use require special glass slides? Any info you can provide on it's use ASAP will be much appreciated. Thank you kindly, Atoska From asmith <@t> mail.barry.edu Tue Apr 7 14:40:35 2009 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Apr 7 14:42:18 2009 Subject: [Histonet] RE: I'm outta here Message-ID: I can spot spam and o.t. posts and delete them in a half second each. The questions on Histonet make me aware of the extent of use of special stains, and the potential for new stains. Jjob postings are forwarded to the career service office here. The safety warnings are often very useful. HIstonet is a great source of information on antigen retrieval techniques and antibodies. I would really miss Histonet. --Allen A Smith, Ph.D. Barry University School of Podiatric Medicine From DKnutson <@t> primecare.org Tue Apr 7 15:06:13 2009 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Tue Apr 7 15:06:05 2009 Subject: [Histonet] Controls needed! Message-ID: <4F0B7161A6CD524FAD8017D52E1553400A768881@exchangent> We are looking for H. Pylori control tissue and also GMS/fungus control tissue. Is there anyone out there that might have extra to share? We have good GRAM control blocks that we would be happy to exchange. Please let me know if you can help us out. Thank you! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, N. Dak. 58506 701-530-6730 Fax 701-530-6735 From gp62 <@t> georgetown.edu Tue Apr 7 15:59:58 2009 From: gp62 <@t> georgetown.edu (Guillermo Palchik) Date: Tue Apr 7 16:00:03 2009 Subject: [Histonet] Fixation question - Cerebellar granular cells Message-ID: Dear Histoneters, I am doing some IHC on rat cerebellar granular cells (NOT TISSUE) and I need to fix them with PF. I have gotten 4 different answers on how to go about this, and I wanted to run this by the list to see what you think. 1) Fix in COLD, 4% PF in 1x PBS 2) Fix in COLD, 4% PF in 1x PBS with 4% Sucrose. 3) Fix in WARM (37 C) ,in 4% PF in 1x PBS. 4) Fix in WARM (37 C), in 1x PBS with 4% Sucrose. I get the fact that the PF might need to be at 37 C, since it is the temperature that the cells are in the incubator and it would probably temperature shock them. What about the sucrose? does it remove the water? I'd appreciate your thoughts... Best, Guillermo Guillermo Palchik gp62@georgetown.edu From Rcartun <@t> harthosp.org Tue Apr 7 17:30:04 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Apr 7 17:30:15 2009 Subject: [Histonet] Recycled formalin Message-ID: <49DB9BAC020000770000AC8F@gwmail4.harthosp.org> Is anyone using recycled formalin for primary fixation of either surgical or autopsy tissue? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From AnthonyH <@t> chw.edu.au Tue Apr 7 17:44:12 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 7 17:44:18 2009 Subject: [Histonet] Unsubscribe In-Reply-To: <397EBB11B9C649428A1AB64BC16C66AA01CC18C0@EXCHANGE2.university.brighton.ac.uk> Message-ID: Sophie, I for one would never throw a torrent of abuse at any one (mmm unless they are politicians - in between football seasons!) So if my (and most others on Histonet) comments are not of some worth then we apologise. We need to try harder. (also please remember the delete key, I unfortunately have to regularly use it) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of S.J.Ainsworth@bsms.ac.uk Sent: Tuesday, 7 April 2009 5:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Unsubscribe Please can you unsubscribe me from this mailing list. As an inexperienced histologist trying to find my feet I no longer feel like I could ask any questions as I'm sure I would get a torrent of abuse back instead of any helpful comments. Sophie Ainsworth Brighton and Sussex Medical School Medical Research Building Falmer East Sussex BN1 9PX Tel: #44 1273 877886 Fax: #44 1273 877884 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Tue Apr 7 17:48:53 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 7 17:49:02 2009 Subject: [Histonet] gi microwave processing In-Reply-To: Message-ID: Nancy, Best advice I can offer is to ensure as much fixation as you can. We start with 30 minutes at 30oC and the ramp it up to 40oC for 30-60 minutes, rinse in 70% ethanol (5min)then continue microwave processing with isopropanol and wax. You may also consider decreasing the processing temperatures. GI biopsies being quite small do not need over-the-top processing, but in my experience, fixation is still the key. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MAGODLEY@aol.com Sent: Tuesday, 7 April 2009 10:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] gi microwave processing I am starting a gi lab and using a microwave processor for my first time. Any suggestions? SHUR Wave processor. Thanks, Nancy **************A Good Credit Score is 700 or Above. See yours in just 2 easy steps! (http://pr.atwola.com/promoclk/100126575x1221621488x1201450096/aol?redir =http:%2F%2Fwww.freecreditreport.com%2Fpm%2Fdefault.aspx%3Fsc%3D668072%2 6hmpgID %3D62%26bcd%3DAprilfooterNO62) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Tue Apr 7 19:06:52 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 7 19:06:56 2009 Subject: [Histonet] AE1/AE3 nuclear staining In-Reply-To: <650B78513FAF9D41B8AE22F991AFCA62024D740D@mck235.averamail.net> Message-ID: Possibly the concentration of hydrogen peroxide in the DAB solution has been slowly increasing (maybe micropipette creep?) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tammy Barnhart Sent: Wednesday, 8 April 2009 2:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AE1/AE3 nuclear staining We have recently been having dark nuclear staining with our AE1/AE3 antibody. This is a recent event and we cannot figure out what is going on. It started gradually and has been increasing in intensity. We have not changed antibody lots or any other part of our protocol. Has anyone seen this before? Any suggestions on what is happening here? Tammy Barnhart, BS, HTL(ASCP) Avera McKennan Hospital ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From gvdobbin <@t> ihis.org Tue Apr 7 19:55:26 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Tue Apr 7 19:55:58 2009 Subject: [Histonet] Recycled formalin Message-ID: Yes. ?? Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Richard Cartun" 04/07/09 7:30 PM >>> Is anyone using recycled formalin for primary fixation of either surgical or autopsy tissue? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From tifei <@t> foxmail.com Tue Apr 7 22:07:21 2009 From: tifei <@t> foxmail.com (TF) Date: Tue Apr 7 22:08:09 2009 Subject: [Histonet] Fixation question - Cerebellar granular cells References: Message-ID: <200904081107164484802@foxmail.com> SGksIGkgbm9ybWFsbHkgd29yayBvbiB0aXNzdWUuIA0KSSB0aGluayA0JSBQRkEgaW4gMC4xTSBQ QiBpcyBmaW5lLiBzbyBhcHByb2FjaCAzLg0KDQoNCjIwMDktMDQtMDggDQoNCg0KDQpURiANCg0K DQoNCreivP7Iy6O6IEd1aWxsZXJtbyBQYWxjaGlrIA0Kt6LLzcqxvOSjuiAyMDA5LTA0LTA4ICAw NTowMzoxMyANCsrVvP7Iy6O6IGhpc3RvbmV0IA0Ks63LzaO6IA0K1vfM4qO6IFtIaXN0b25ldF0g Rml4YXRpb24gcXVlc3Rpb24gLSBDZXJlYmVsbGFyIGdyYW51bGFyIGNlbGxzIA0KIA0KRGVhciBI aXN0b25ldGVycywNCkkgYW0gZG9pbmcgc29tZSBJSEMgb24gcmF0IGNlcmViZWxsYXIgZ3JhbnVs YXIgY2VsbHMgKE5PVCBUSVNTVUUpIGFuZCAgDQpJIG5lZWQgdG8gZml4IHRoZW0gd2l0aCBQRi4N CkkgaGF2ZSBnb3R0ZW4gNCBkaWZmZXJlbnQgYW5zd2VycyBvbiBob3cgdG8gZ28gYWJvdXQgdGhp cywgYW5kIEkgIA0Kd2FudGVkIHRvIHJ1biB0aGlzIGJ5IHRoZSBsaXN0IHRvIHNlZSB3aGF0IHlv dSB0aGluay4NCjEpIEZpeCBpbiBDT0xELCA0JSBQRiBpbiAxeCBQQlMNCjIpIEZpeCBpbiBDT0xE LCA0JSBQRiBpbiAxeCBQQlMgd2l0aCA0JSBTdWNyb3NlLg0KMykgRml4IGluIFdBUk0gKDM3IEMp ICxpbiAgNCUgUEYgaW4gMXggUEJTLg0KNCkgRml4IGluIFdBUk0gKDM3IEMpLCBpbiAxeCBQQlMg d2l0aCA0JSBTdWNyb3NlLg0KSSBnZXQgdGhlIGZhY3QgdGhhdCB0aGUgUEYgbWlnaHQgbmVlZCB0 byBiZSBhdCAzNyBDLCBzaW5jZSBpdCBpcyB0aGUgIA0KdGVtcGVyYXR1cmUgdGhhdCB0aGUgY2Vs bHMgYXJlIGluIHRoZSBpbmN1YmF0b3IgYW5kIGl0IHdvdWxkIHByb2JhYmx5ICANCnRlbXBlcmF0 dXJlIHNob2NrIHRoZW0uIFdoYXQgYWJvdXQgdGhlIHN1Y3Jvc2U/IGRvZXMgaXQgcmVtb3ZlIHRo ZSAgDQp3YXRlcj8NCkknZCBhcHByZWNpYXRlIHlvdXIgdGhvdWdodHMuLi4NCkJlc3QsDQpHdWls bGVybW8NCkd1aWxsZXJtbyBQYWxjaGlrDQpncDYyQGdlb3JnZXRvd24uZWR1DQpfX19fX19fX19f X19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fXw0KSGlzdG9uZXQgbWFpbGluZyBs aXN0DQpIaXN0b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUNCmh0dHA6Ly9saXN0cy51dHNv dXRod2VzdGVybi5lZHUvbWFpbG1hbi9saXN0aW5mby9oaXN0b25ldA0K From bernietaupin <@t> ymail.com Tue Apr 7 22:40:32 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Tue Apr 7 22:42:07 2009 Subject: [Histonet] Staying... In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE1BF0511@LTA3VS011.ees.hhs.gov> References: <0KHQ0015TBFDE890@ksle966mailxc2.everestkc.net> <9A16CB5D55FC1648ADF11B63E72A1BE1BF0511@LTA3VS011.ees.hhs.gov> Message-ID: <69593.25777.qm@web43509.mail.sp1.yahoo.com> Yes, Well-done to all of you who have decided to stay! It really separates the wheat from the chaff, as it were. Or the weenies from the real adults, more or less. And even the French-speakers from the non-French-speakers, in the case of Kemlo/Rene. Ha ha. That was a joke. Learn to laugh a little, folks. I, myself, have decided to stay. I'll try to play more nicely. Here's hoping the rest of you can be so cool about it all. SO BIG UPS TO ALL THE HISTOLOGISTS IN THE HOUSE! CAN I GET A WHUT-WHUT! ________________________________ From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: Marla Thomas ; histonet@lists.utsouthwestern.edu Sent: Tuesday, April 7, 2009 8:10:22 AM Subject: RE: [Histonet] Staying... Amen! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marla Thomas Sent: Tuesday, April 07, 2009 7:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staying... I am staying on the list-serve. I have learned a lot from people on this list-serve. I have been in the field since 1970 and I too am still learning things. No one has all of the answers, and sometimes there isn't just one answer. I am staying on the list-serve, and if my input can help one person, or 15 different responses can help me, then the list-serve works. There are still people out there asking questions in between the bickering. Marla Thomas, HT(ASCP) Litton Pathology Associates, PC 700 NW Hunter Dr. Blue Springs, MO 64015 Phone: 816-229-6449 Fax:816-874-4400 CONFIDENTIALITY NOTICE This message and any included attachments are from Litton Pathology Associates, P.C. and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 816-229-6449 and ask for the HIPAA/Compliance Coordinator. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Tue Apr 7 22:40:32 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Tue Apr 7 22:47:19 2009 Subject: [Histonet] Staying... In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE1BF0511@LTA3VS011.ees.hhs.gov> References: <0KHQ0015TBFDE890@ksle966mailxc2.everestkc.net> <9A16CB5D55FC1648ADF11B63E72A1BE1BF0511@LTA3VS011.ees.hhs.gov> Message-ID: <69593.25777.qm@web43509.mail.sp1.yahoo.com> Yes, Well-done to all of you who have decided to stay! It really separates the wheat from the chaff, as it were. Or the weenies from the real adults, more or less. And even the French-speakers from the non-French-speakers, in the case of Kemlo/Rene. Ha ha. That was a joke. Learn to laugh a little, folks. I, myself, have decided to stay. I'll try to play more nicely. Here's hoping the rest of you can be so cool about it all. SO BIG UPS TO ALL THE HISTOLOGISTS IN THE HOUSE! CAN I GET A WHUT-WHUT! ________________________________ From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: Marla Thomas ; histonet@lists.utsouthwestern.edu Sent: Tuesday, April 7, 2009 8:10:22 AM Subject: RE: [Histonet] Staying... Amen! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marla Thomas Sent: Tuesday, April 07, 2009 7:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staying... I am staying on the list-serve. I have learned a lot from people on this list-serve. I have been in the field since 1970 and I too am still learning things. No one has all of the answers, and sometimes there isn't just one answer. I am staying on the list-serve, and if my input can help one person, or 15 different responses can help me, then the list-serve works. There are still people out there asking questions in between the bickering. Marla Thomas, HT(ASCP) Litton Pathology Associates, PC 700 NW Hunter Dr. Blue Springs, MO 64015 Phone: 816-229-6449 Fax:816-874-4400 CONFIDENTIALITY NOTICE This message and any included attachments are from Litton Pathology Associates, P.C. and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 816-229-6449 and ask for the HIPAA/Compliance Coordinator. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lactose.intolerant <@t> yahoo.com Tue Apr 7 22:56:34 2009 From: lactose.intolerant <@t> yahoo.com (aa aa) Date: Tue Apr 7 22:56:38 2009 Subject: [Histonet] Unsubscribe In-Reply-To: Message-ID: <143187.83928.qm@web55306.mail.re4.yahoo.com> Dear Histonetters: It sure is a real shame to see so many familiar names leave the list just due to a little annoyance. Sorry to send yet *another* note about all of this to the whole list, but I wanted to pass on a link my supervisor sent to me when we were having similar real-life crisis in my lab... it really does put things into perfect perspective: http://smouch.net/lol/ Have a great day everyone, and keep HistoNetting! ~L. --- On Tue, 4/7/09, Tony Henwood wrote: From: Tony Henwood Subject: RE: [Histonet] Unsubscribe To: S.J.Ainsworth@bsms.ac.uk, histonet@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 6:44 PM Sophie, I for one would never throw a torrent of abuse at any one (mmm unless they are politicians - in between football seasons!) So if my (and most others on Histonet) comments are not of some worth then we apologise. We need to try harder. (also please remember the delete key, I unfortunately have to regularly use it) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of S.J.Ainsworth@bsms.ac.uk Sent: Tuesday, 7 April 2009 5:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Unsubscribe Please can you unsubscribe me from this mailing list. As an inexperienced histologist trying to find my feet I no longer feel like I could ask any questions as I'm sure I would get a torrent of abuse back instead of any helpful comments. Sophie Ainsworth Brighton and Sussex Medical School Medical Research Building Falmer East Sussex BN1 9PX Tel: #44 1273 877886 Fax: #44 1273 877884 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Tue Apr 7 23:07:13 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Tue Apr 7 23:07:17 2009 Subject: [Histonet] Whole human feet In-Reply-To: <33b7cf2b0904061704g541684bdtd21d4dcd76469661@mail.gmail.com> References: <33b7cf2b0904061704g541684bdtd21d4dcd76469661@mail.gmail.com> Message-ID: <633861.20535.qm@web43505.mail.sp1.yahoo.com> No offense, dude, but GROSS. That's probably why nobody has bitten yet, in regards to this query. ________________________________ From: Yak-Nam Wang To: histonet@lists.utsouthwestern.edu Sent: Monday, April 6, 2009 8:04:54 PM Subject: [Histonet] Whole human feet Hello all, If possible, we would like to obtain histological sections of adult human feet (plane of the anterior-posterior surface). Does anyone know of labs/groups that have done this or something similar? Thank you for your help Yak-Nam Wang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Tue Apr 7 23:06:44 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Tue Apr 7 23:08:10 2009 Subject: [Histonet] "FREEZY" spray In-Reply-To: References: Message-ID: <82489.18598.qm@web43513.mail.sp1.yahoo.com> > Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it! I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen. kisses, flowers and rainbows, Bernie Taupin, King of Cryomicrotomy, Esq. From akemiat3377 <@t> yahoo.com Wed Apr 8 00:03:04 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Apr 8 00:03:07 2009 Subject: [Histonet] "FREEZY" spray Message-ID: <610276.4454.qm@web31301.mail.mud.yahoo.com> Bernie, I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. ? Years later, I developed the "MATSSE (pH3.4) 1-Step Trichrome Stain Kit" (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. ?This kit has long since discontinued by the way...This came from Biocare's Data Sheet. NOTE: Muscle tissue should be suitably obtained and frozen within 30 minutes after excision.? The usual method of freezing is to first mount a transverse section of the muscle on a chuck using 10% tragacanth gum or equivalent commercial frozen section mounting media as the adhesive.? The chuck with the mounted specimen is then immersed in prechilled isopentane until frozen.? Isopentane is precooled when a container of the substance is placed into liquid nitrogen.? At a temperature of -160? C, the isopentane has a slightly syrupy consistency.? Care should be taken to remove the muscle sample when freezing is complete, usually after 25 to 30 seconds.? Too short a freezing time produces artifacts; prolonged freezing can produce cracking of the block. ? NOTE:?If the sample cannot be sectioned immediately after freezing, it may be wrapped in foil and placed in a plastic-lidded container along with a small amount of ice for moisture.? Specimens may be stored at -70? C until sectioned.? Regards,Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com --- On Tue, 4/7/09, Bernie Taupin wrote: From: Bernie Taupin Subject: Re: [Histonet] "FREEZY" spray To: "Jennifer MacDonald" , "Ingles Claire" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 9:06 PM > Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it! I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen. kisses, flowers and rainbows, Bernie Taupin, King of Cryomicrotomy, Esq. ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Wed Apr 8 00:11:53 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 8 00:11:57 2009 Subject: [Histonet] "FREEZY" spray In-Reply-To: <610276.4454.qm@web31301.mail.mud.yahoo.com> References: <610276.4454.qm@web31301.mail.mud.yahoo.com> Message-ID: <422706.46324.qm@web43512.mail.sp1.yahoo.com> I use isopentane suspended in liquid nitrogen, too. sorry if this sounds curt, but due to your cut-and-pasting, im not entirely sure what point youre trying to get at...? ________________________________ From: Akemi Allison-Tacha To: Jennifer MacDonald ; Ingles Claire ; Bernie Taupin Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:03:04 AM Subject: Re: [Histonet] "FREEZY" spray Bernie, I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. Years later, I developed the "MATSSE (pH3.4) 1-Step Trichrome Stain Kit" (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. This kit has long since discontinued by the way....This came from Biocare's Data Sheet. NOTE:Muscle tissue should be suitably obtained and frozen within 30 minutes after excision. The usual method of freezing is to first mount a transverse section of the muscle on a chuck using 10% tragacanth gum or equivalent commercial frozen section mounting media as the adhesive. The chuck with the mounted specimen is then immersed in prechilled isopentane until frozen. Isopentane is precooled when a container of the substance is placed into liquid nitrogen. At a temperature of -160? C, the isopentane has a slightly syrupy consistency. Care should be taken to remove the muscle sample when freezing is complete, usually after 25 to 30 seconds. Too short a freezing time produces artifacts; prolonged freezing can produce cracking of the block. NOTE: If the sample cannot be sectioned immediately after freezing, it may be wrapped in foil and placed in a plastic-lidded container along with a small amount of ice for moisture. Specimens may be stored at -70? C until sectioned. Regards, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com --- On Tue, 4/7/09, Bernie Taupin wrote: From: Bernie Taupin Subject: Re: [Histonet] "FREEZY" spray To: "Jennifer MacDonald" , "Ingles Claire" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 9:06 PM > Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it! I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen. kisses, flowers and rainbows, Bernie Taupin, King of Cryomicrotomy, Esq. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern..edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Apr 8 00:27:35 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Apr 8 00:27:40 2009 Subject: [Histonet] "FREEZY" spray Message-ID: <984458.1466.qm@web31307.mail.mud.yahoo.com> Sorry, I too noticed that the text looked weird then I did a cut and paste. ?I keep all the Data Sheets I created in my files, and this was just a portion of the Notes from the original Data sheet. ?I thought it might be helpful because you stated you had difficulty with cracking.? You did not mention using isopentane. ?Some people?immerse?the tissue straight into liquid nitrogen, which could cause freezing artifacts. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com --- On Tue, 4/7/09, Bernie Taupin wrote: From: Bernie Taupin Subject: Re: [Histonet] "FREEZY" spray To: "Akemi Allison-Tacha" , "Jennifer MacDonald" , "Ingles Claire" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 10:11 PM I use isopentane suspended in liquid nitrogen, too. sorry if this sounds curt, but due to your cut-and-pasting, im not entirely sure what point youre trying to get at...? From: Akemi Allison-Tacha To: Jennifer MacDonald ; Ingles Claire ; Bernie Taupin Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:03:04 AM Subject: Re: [Histonet] "FREEZY" spray Bernie, I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. ? Years later, I developed the "MATSSE (pH3.4) 1-Step Trichrome Stain Kit" (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. ?This kit has long since discontinued by the way...This came from Biocare's Data Sheet. NOTE: Muscle tissue should be suitably obtained and frozen within 30 minutes after excision.? The usual method of freezing is to first mount a transverse section of the muscle on a chuck using 10% tragacanth gum or equivalent commercial frozen section mounting media as the adhesive.? The chuck with the mounted specimen is then immersed in prechilled isopentane until frozen.? Isopentane is precooled when a container of the substance is placed into liquid nitrogen.? At a temperature of -160? C, the isopentane has a slightly syrupy consistency.? Care should be taken to remove the muscle sample when freezing is complete, usually after 25 to 30 seconds.? Too short a freezing time produces artifacts; prolonged freezing can produce cracking of the block. ? NOTE:?If the sample cannot be sectioned immediately after freezing, it may be wrapped in foil and placed in a plastic-lidded container along with a small amount of ice for moisture.? Specimens may be stored at -70? C until sectioned..? Regards,Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo..com --- On Tue, 4/7/09, Bernie Taupin wrote: From: Bernie Taupin Subject: Re: [Histonet] "FREEZY" spray To: "Jennifer MacDonald" , "Ingles Claire" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 9:06 PM > Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it! I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen. kisses, flowers and rainbows, Bernie Taupin, King of Cryomicrotomy, Esq. ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Wed Apr 8 00:38:00 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 8 00:39:08 2009 Subject: [Histonet] "FREEZY" spray In-Reply-To: <984458.1466.qm@web31307.mail.mud.yahoo.com> References: <984458.1466.qm@web31307.mail.mud.yahoo.com> Message-ID: <958249.26146.qm@web43503.mail.sp1.yahoo.com> Sorry, again, I'm confused... are you responding to me? I'm not the original poster... I never stated I had trouble with cracking, because, well, I don't. I'm Bernie Taupin, the King of Cryomicrotomy, Esq. ________________________________ From: Akemi Allison-Tacha To: Jennifer MacDonald ; Ingles Claire ; Bernie Taupin Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:27:35 AM Subject: Re: [Histonet] "FREEZY" spray Sorry, I too noticed that the text looked weird then I did a cut and paste. I keep all the Data Sheets I created in my files, and this was just a portion of the Notes from the original Data sheet. I thought it might be helpful because you stated you had difficulty with cracking. You did not mention using isopentane. Some people immerse the tissue straight into liquid nitrogen, which could cause freezing artifacts. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com --- On Tue, 4/7/09, Bernie Taupin wrote: From: Bernie Taupin Subject: Re: [Histonet] "FREEZY" spray To: "Akemi Allison-Tacha" , "Jennifer MacDonald" , "Ingles Claire" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 10:11 PM I use isopentane suspended in liquid nitrogen, too. sorry if this sounds curt, but due to your cut-and-pasting, im not entirely sure what point youre trying to get at...? ________________________________ From: Akemi Allison-Tacha To: Jennifer MacDonald ; Ingles Claire ; Bernie Taupin Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:03:04 AM Subject: Re: [Histonet] "FREEZY" spray Bernie, I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. Years later, I developed the "MATSSE (pH3.4) 1-Step Trichrome Stain Kit" (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. This kit has long since discontinued by the way...This came from Biocare's Data Sheet. NOTE:Muscle tissue should be suitably obtained and frozen within 30 minutes after excision. The usual method of freezing is to first mount a transverse section of the muscle on a chuck using 10% tragacanth gum or equivalent commercial frozen section mounting media as the adhesive. The chuck with the mounted specimen is then immersed in prechilled isopentane until frozen. Isopentane is precooled when a container of the substance is placed into liquid nitrogen. At a temperature of -160? C, the isopentane has a slightly syrupy consistency. Care should be taken to remove the muscle sample when freezing is complete, usually after 25 to 30 seconds.. Too short a freezing time produces artifacts; prolonged freezing can produce cracking of the block. NOTE: If the sample cannot be sectioned immediately after freezing, it may be wrapped in foil and placed in a plastic-lidded container along with a small amount of ice for moisture. Specimens may be stored at -70? C until sectioned.. Regards, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo..com --- On Tue, 4/7/09, Bernie Taupin wrote: From: Bernie Taupin Subject: Re: [Histonet] "FREEZY" spray To: "Jennifer MacDonald" , "Ingles Claire" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 9:06 PM > Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it! I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen. kisses, flowers and rainbows, Bernie Taupin, King of Cryomicrotomy, Esq. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Wed Apr 8 02:58:22 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Apr 8 02:58:31 2009 Subject: [Histonet] "FREEZY" spray In-Reply-To: <958249.26146.qm@web43503.mail.sp1.yahoo.com> Message-ID: Hey, Would you believe that putting the word "Taupin" into my outlook rules actually works. Now every obnoxious post from "Taupin" actually disappears into my Junk folder and I do not have to read the drivel. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, 8 April 2009 3:38 PM To: Akemi Allison-Tacha; Jennifer MacDonald; Ingles Claire Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] "FREEZY" spray Sorry, again, I'm confused... are you responding to me? I'm not the original poster... I never stated I had trouble with cracking, because, well, I don't. I'm Bernie Taupin, the King of Cryomicrotomy, Esq. ________________________________ From: Akemi Allison-Tacha To: Jennifer MacDonald ; Ingles Claire ; Bernie Taupin Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:27:35 AM Subject: Re: [Histonet] "FREEZY" spray Sorry, I too noticed that the text looked weird then I did a cut and paste. I keep all the Data Sheets I created in my files, and this was just a portion of the Notes from the original Data sheet. I thought it might be helpful because you stated you had difficulty with cracking. You did not mention using isopentane. Some people immerse the tissue straight into liquid nitrogen, which could cause freezing artifacts. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com --- On Tue, 4/7/09, Bernie Taupin wrote: From: Bernie Taupin Subject: Re: [Histonet] "FREEZY" spray To: "Akemi Allison-Tacha" , "Jennifer MacDonald" , "Ingles Claire" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 10:11 PM I use isopentane suspended in liquid nitrogen, too. sorry if this sounds curt, but due to your cut-and-pasting, im not entirely sure what point youre trying to get at...? ________________________________ From: Akemi Allison-Tacha To: Jennifer MacDonald ; Ingles Claire ; Bernie Taupin Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:03:04 AM Subject: Re: [Histonet] "FREEZY" spray Bernie, I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. Years later, I developed the "MATSSE (pH3.4) 1-Step Trichrome Stain Kit" (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. This kit has long since discontinued by the way...This came from Biocare's Data Sheet. NOTE:Muscle tissue should be suitably obtained and frozen within 30 minutes after excision. The usual method of freezing is to first mount a transverse section of the muscle on a chuck using 10% tragacanth gum or equivalent commercial frozen section mounting media as the adhesive. The chuck with the mounted specimen is then immersed in prechilled isopentane until frozen. Isopentane is precooled when a container of the substance is placed into liquid nitrogen. At a temperature of -160? C, the isopentane has a slightly syrupy consistency. Care should be taken to remove the muscle sample when freezing is complete, usually after 25 to 30 seconds.. Too short a freezing time produces artifacts; prolonged freezing can produce cracking of the block. NOTE: If the sample cannot be sectioned immediately after freezing, it may be wrapped in foil and placed in a plastic-lidded container along with a small amount of ice for moisture. Specimens may be stored at -70? C until sectioned.. Regards, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo..com --- On Tue, 4/7/09, Bernie Taupin wrote: From: Bernie Taupin Subject: Re: [Histonet] "FREEZY" spray To: "Jennifer MacDonald" , "Ingles Claire" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 9:06 PM > Does this apply to Liquid Nitrogen as well? I'd hate to have to try to > cut fatty sections in the cryostat without it! I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen. kisses, flowers and rainbows, Bernie Taupin, King of Cryomicrotomy, Esq. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Apr 8 03:05:39 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Apr 8 03:05:50 2009 Subject: [Histonet] "FREEZY" spray Message-ID: <86ADE4EB583CE64799A9924684A0FBBF06833F5F@wahtntex2.waht.swest.nhs.uk> Say thank you Uncle Kemlo!! Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: 08 April 2009 08:58 To: Bernie Taupin; Akemi Allison-Tacha; Jennifer MacDonald; Ingles Claire Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] "FREEZY" spray Hey, Would you believe that putting the word "Taupin" into my outlook rules actually works. Now every obnoxious post from "Taupin" actually disappears into my Junk folder and I do not have to read the drivel. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, 8 April 2009 3:38 PM To: Akemi Allison-Tacha; Jennifer MacDonald; Ingles Claire Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] "FREEZY" spray Sorry, again, I'm confused... are you responding to me? I'm not the original poster... I never stated I had trouble with cracking, because, well, I don't. I'm Bernie Taupin, the King of Cryomicrotomy, Esq. ________________________________ From: Akemi Allison-Tacha To: Jennifer MacDonald ; Ingles Claire ; Bernie Taupin Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:27:35 AM Subject: Re: [Histonet] "FREEZY" spray Sorry, I too noticed that the text looked weird then I did a cut and paste. I keep all the Data Sheets I created in my files, and this was just a portion of the Notes from the original Data sheet. I thought it might be helpful because you stated you had difficulty with cracking. You did not mention using isopentane. Some people immerse the tissue straight into liquid nitrogen, which could cause freezing artifacts. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com --- On Tue, 4/7/09, Bernie Taupin wrote: From: Bernie Taupin Subject: Re: [Histonet] "FREEZY" spray To: "Akemi Allison-Tacha" , "Jennifer MacDonald" , "Ingles Claire" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 10:11 PM I use isopentane suspended in liquid nitrogen, too. sorry if this sounds curt, but due to your cut-and-pasting, im not entirely sure what point youre trying to get at...? ________________________________ From: Akemi Allison-Tacha To: Jennifer MacDonald ; Ingles Claire ; Bernie Taupin Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:03:04 AM Subject: Re: [Histonet] "FREEZY" spray Bernie, I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. Years later, I developed the "MATSSE (pH3.4) 1-Step Trichrome Stain Kit" (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. This kit has long since discontinued by the way...This came from Biocare's Data Sheet. NOTE:Muscle tissue should be suitably obtained and frozen within 30 minutes after excision. The usual method of freezing is to first mount a transverse section of the muscle on a chuck using 10% tragacanth gum or equivalent commercial frozen section mounting media as the adhesive. The chuck with the mounted specimen is then immersed in prechilled isopentane until frozen. Isopentane is precooled when a container of the substance is placed into liquid nitrogen. At a temperature of -160? C, the isopentane has a slightly syrupy consistency. Care should be taken to remove the muscle sample when freezing is complete, usually after 25 to 30 seconds.. Too short a freezing time produces artifacts; prolonged freezing can produce cracking of the block. NOTE: If the sample cannot be sectioned immediately after freezing, it may be wrapped in foil and placed in a plastic-lidded container along with a small amount of ice for moisture. Specimens may be stored at -70? C until sectioned.. Regards, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo..com --- On Tue, 4/7/09, Bernie Taupin wrote: From: Bernie Taupin Subject: Re: [Histonet] "FREEZY" spray To: "Jennifer MacDonald" , "Ingles Claire" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 9:06 PM > Does this apply to Liquid Nitrogen as well? I'd hate to have to try to > cut fatty sections in the cryostat without it! I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen. kisses, flowers and rainbows, Bernie Taupin, King of Cryomicrotomy, Esq. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From njoydobro <@t> aol.com Wed Apr 8 06:58:00 2009 From: njoydobro <@t> aol.com (njoydobro@aol.com) Date: Wed Apr 8 06:58:15 2009 Subject: [Histonet] Staying... In-Reply-To: <69593.25777.qm@web43509.mail.sp1.yahoo.com> References: <0KHQ0015TBFDE890@ksle966mailxc2.everestkc.net><9A16CB5D55FC1648ADF11B63E72A1BE1BF0511@LTA3VS011.ees.hhs.gov> <69593.25777.qm@web43509.mail.sp1.yahoo.com> Message-ID: <8CB86586605F0BA-BA8-2C37@webmail-db04.sysops.aol.com> is your name really Bernie Taupin or are? you just a?fan of his? Gene -----Original Message----- From: Bernie Taupin To: Bartlett, Jeanine (CDC/CCID/NCZVED) ; Marla Thomas ; histonet@lists.utsouthwestern.edu Sent: Tue, 7 Apr 2009 11:40 pm Subject: Re: [Histonet] Staying... Yes, Well-done to all of you who have decided to stay! It really separates the wheat from the chaff, as it were. Or the weenies from the real adults, more or less. And even the French-speakers from the non-French-speakers, in the case of Kemlo/Rene. Ha ha. That was a joke. Learn to laugh a little, folks. I, myself, have decided to stay. I'll try to play more nicely. Here's hoping the rest of you can be so cool about it all. SO BIG UPS TO ALL THE HISTOLOGISTS IN THE HOUSE! CAN I GET A WHUT-WHUT! ________________________________ From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: Marla Thomas ; histonet@lists.utsouthwestern.edu Sent: Tuesday, April 7, 2009 8:10:22 AM Subject: RE: [Histonet] Staying... Amen! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marla Thomas Sent: Tuesday, April 07, 2009 7:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staying... I am staying on the list-serve. I have learned a lot from people on this list-serve. I have been in the field since 1970 and I too am still learning things. No one has all of the answers, and sometimes there isn't just one answer. I am staying on the list-serve, and if my input can help one person, or 15 different responses can help me, then the list-serve works. There are still people out there asking questions in between the bickering. Marla Thomas, HT(ASCP) Litton Pathology Associates, PC 700 NW Hunter Dr. Blue Springs, MO 64015 Phone: 816-229-6449 Fax:816-874-4400 CONFIDENTIALITY NOTICE This message and any included attachments are from Litton Pathology Associates, P.C. and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwardin g, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 816-229-6449 and ask for the HIPAA/Compliance Coordinator. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed Apr 8 07:14:21 2009 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed Apr 8 07:14:26 2009 Subject: [Histonet] Controls needed! In-Reply-To: <4F0B7161A6CD524FAD8017D52E1553400A768881@exchangent> References: <4F0B7161A6CD524FAD8017D52E1553400A768881@exchangent> Message-ID: <5F3F860CFE0F4741B1D87A88A58FAE9A256101@mailbe01.mc.vanderbilt.edu> Hi Deanne, Have you checked with the NSH Control Tissue Bank? The form to request tissue is online on the NSH web page (www.nsh.org). The bank is run by the Quality Control committee in conjunction with the IHCRG. It is a free service to NSH members. You may contact the committee chair, William DeSalvo at wdesalvo.cac@hotmail.com with any specific questions. Have a great week. Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: Knutson, Deanne [mailto:DKnutson@primecare.org] Sent: Tuesday, April 07, 2009 3:06 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Controls needed! We are looking for H. Pylori control tissue and also GMS/fungus control tissue. Is there anyone out there that might have extra to share? We have good GRAM control blocks that we would be happy to exchange. Please let me know if you can help us out. Thank you! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, N. Dak. 58506 701-530-6730 Fax 701-530-6735 From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Apr 8 07:50:41 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Apr 8 07:50:46 2009 Subject: [Histonet] Controls needed! Message-ID: <86ADE4EB583CE64799A9924684A0FBBF06833FD3@wahtntex2.waht.swest.nhs.uk> It's just a thought, can't you get the control from your Microbiology Lab? You just need to make a cell block out of the bacteria that they've grown; I'm not aware that Hpylori has to go through a human system before you can demonstrate it. Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hofecker, Jennifer L Sent: 08 April 2009 13:14 To: Knutson, Deanne; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls needed! Hi Deanne, Have you checked with the NSH Control Tissue Bank? The form to request tissue is online on the NSH web page (www.nsh.org). The bank is run by the Quality Control committee in conjunction with the IHCRG. It is a free service to NSH members. You may contact the committee chair, William DeSalvo at wdesalvo.cac@hotmail.com with any specific questions. Have a great week. Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: Knutson, Deanne [mailto:DKnutson@primecare.org] Sent: Tuesday, April 07, 2009 3:06 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Controls needed! We are looking for H. Pylori control tissue and also GMS/fungus control tissue. Is there anyone out there that might have extra to share? We have good GRAM control blocks that we would be happy to exchange. Please let me know if you can help us out. Thank you! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, N. Dak. 58506 701-530-6730 Fax 701-530-6735 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dchihc <@t> yahoo.com Wed Apr 8 08:43:36 2009 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Wed Apr 8 08:50:30 2009 Subject: [Histonet] Benchmark XT Message-ID: <332647.97028.qm@web43512.mail.sp1.yahoo.com> Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with? needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 ?hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 From algranth <@t> email.arizona.edu Wed Apr 8 09:18:30 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Wed Apr 8 09:18:40 2009 Subject: [Histonet] histonet off-topic comments In-Reply-To: <49DB3DD7.F783.00DA.0@childrens.com> References: <200904062309.n36N9lYQ009723@smtp.ufl.edu> <49DB586C.9000608@ufl.edu> <49DB3DD7.F783.00DA.0@childrens.com> Message-ID: <3BCF841B-461C-4519-99CB-EA83DDA281E0@email.arizona.edu> Linda, This really is too much - now I have this thing on my computer maliciously put there by "aa aa" that I can not get to close. More than one person has to be removed from the list and if Bernie was removed why are there emails from him this morning? Histonet has helped me so much but I'm afraid this is the last straw. I'm going to have to unsubscribe. Sad to see such a wonderful resource go down the tubes because of a handful of inconsiderate bastards. Andi Grantham Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello On Apr 7, 2009, at 9:52 AM, LINDA MARGRAF wrote: > Dear Histonet members: > I have removed Bernie Taupin from the list. > Let's please get back to the topic of histology and related > fields.Thanks > Linda M > Histonet administrator > >>>> MKing 4/7/2009 8:43 AM >>> > a motion is hereby offered to have the active members of histonet vote > this troll off the island, or at least request that the list moderator > do so. this person has proven to have absolutely nothing of value to > contribute to this group, and is only intent on disrupting it. > > second? > > ----------------- > Message: 16 > Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) > From: Bernie Taupin > Subject: Re: [Histonet] Nice one Kemlo > To: histonet@lists.utsouthwestern.edu > Message-ID: <453473.65848.qm@web43516.mail.sp1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > >>> Instead of spitting your venom try to learn > > Sort of like you might consider learning correct English? I mean, its > one thing if its not your first language, but despite how many > people on > here called you kindly and helpful, I find it perplexing that you feel > compelled to call me a "piece of ignorant", and TWICE, no less. > > I do not speak French. This list is not in French. Ergo, my awareness > (or lack thereof) of proper French diction has absolutely nothing at > all > to do with anything... aside form providing you a vehicle to call me > names and lash out. > > If you have something to say, say it. The simple fact that you do not > like me does not give you- or anyone else- wholesale right to insult > me > or call me names. > > You should be ashamed of yourself, Rene. I never expected such > antisocial behavior from you, and frankly, when you behave so > regrettably, I feel no compulsion to "learn" anything form you! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Please consider the > environment before printing this e-mail
>
> > This e-mail, facsimile, or letter and > any files or attachments transmitted with it contains
> information that is confidential and privileged. This information > is intended only for the use of the
> individual(s) and entity(ies) to whom it is addressed. If you are > the intended recipient, further
> > disclosures are prohibited without proper authorization. If you > are not the intended recipient, any
> disclosure, copying, printing, or use of this information is > strictly prohibited and possibly a
> violation of federal or state law and regulations. If you have > received this information in error,
> please notify Children's Medical Center Dallas immediately at > 214-456-4444 or via e-mail at
> privacy@childrens.com. Children's Medical Center Dallas and its > affiliates hereby claim all
> applicable privileges related to this information.
> >
> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From algranth <@t> email.arizona.edu Wed Apr 8 09:21:58 2009 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Wed Apr 8 09:22:02 2009 Subject: [Histonet] UNSUBSCRIBE Message-ID: <0FC09FE5-238D-4D69-A9B9-B5E87E934F6E@email.arizona.edu> UNSUBSCRIBE Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello From mucram11 <@t> comcast.net Wed Apr 8 09:28:49 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Wed Apr 8 09:28:53 2009 Subject: [Histonet] histonet off-topic comments In-Reply-To: <3BCF841B-461C-4519-99CB-EA83DDA281E0@email.arizona.edu> References: <200904062309.n36N9lYQ009723@smtp.ufl.edu> <49DB586C.9000608@ufl.edu> <49DB3DD7.F783.00DA.0@childrens.com> <3BCF841B-461C-4519-99CB-EA83DDA281E0@email.arizona.edu> Message-ID: <001501c9b856$555c4b60$0014e220$@net> AMEN!! Andi - after a while you get tired of first reviewing every message for the good ones and then deleting everything else as fast as you can without opening them. This is too valuable a service to Histology to lose over a few malicious individuals who obviously have no interest in the field or what we do or worse may not even be histologists. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Wednesday, April 08, 2009 10:19 AM To: LINDA MARGRAF Cc: histonet@lists.utsouthwestern.edu; MKing Subject: Re: [Histonet] histonet off-topic comments Linda, This really is too much - now I have this thing on my computer maliciously put there by "aa aa" that I can not get to close. More than one person has to be removed from the list and if Bernie was removed why are there emails from him this morning? Histonet has helped me so much but I'm afraid this is the last straw. I'm going to have to unsubscribe. Sad to see such a wonderful resource go down the tubes because of a handful of inconsiderate bastards. Andi Grantham Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello On Apr 7, 2009, at 9:52 AM, LINDA MARGRAF wrote: > Dear Histonet members: > I have removed Bernie Taupin from the list. > Let's please get back to the topic of histology and related > fields.Thanks > Linda M > Histonet administrator > >>>> MKing 4/7/2009 8:43 AM >>> > a motion is hereby offered to have the active members of histonet vote > this troll off the island, or at least request that the list moderator > do so. this person has proven to have absolutely nothing of value to > contribute to this group, and is only intent on disrupting it. > > second? > > ----------------- > Message: 16 > Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) > From: Bernie Taupin > Subject: Re: [Histonet] Nice one Kemlo > To: histonet@lists.utsouthwestern.edu > Message-ID: <453473.65848.qm@web43516.mail.sp1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > >>> Instead of spitting your venom try to learn > > Sort of like you might consider learning correct English? I mean, its > one thing if its not your first language, but despite how many > people on > here called you kindly and helpful, I find it perplexing that you feel > compelled to call me a "piece of ignorant", and TWICE, no less. > > I do not speak French. This list is not in French. Ergo, my awareness > (or lack thereof) of proper French diction has absolutely nothing at > all > to do with anything... aside form providing you a vehicle to call me > names and lash out. > > If you have something to say, say it. The simple fact that you do not > like me does not give you- or anyone else- wholesale right to insult > me > or call me names. > > You should be ashamed of yourself, Rene. I never expected such > antisocial behavior from you, and frankly, when you behave so > regrettably, I feel no compulsion to "learn" anything form you! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Please consider the > environment before printing this e-mail
>
> > This e-mail, facsimile, or letter and > any files or attachments transmitted with it contains
> information that is confidential and privileged. This information > is intended only for the use of the
> individual(s) and entity(ies) to whom it is addressed. If you are > the intended recipient, further
> > disclosures are prohibited without proper authorization. If you > are not the intended recipient, any
> disclosure, copying, printing, or use of this information is > strictly prohibited and possibly a
> violation of federal or state law and regulations. If you have > received this information in error,
> please notify Children's Medical Center Dallas immediately at > 214-456-4444 or via e-mail at
> privacy@childrens.com. Children's Medical Center Dallas and its > affiliates hereby claim all
> applicable privileges related to this information.
> >
> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Apr 8 10:16:57 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Apr 8 10:17:02 2009 Subject: [Histonet] Re: BEWARE "aa aa" staying with a link Message-ID: <235835.15787.qm@web31304.mail.mud.yahoo.com> Hi,Late last night I saw the posting from??"aa aa"?regarding staying, and there was a link, which I stupidly linked on to. ?What a BIG MISTAKE! ?It is a musical video. ?I started listening to it and it was cute, then I tried to close it. It stayed on, and on, and on! ?I have a Mac Book Pro Laptop, and I couldn't even do a force quit! ?I had to remove the battery and do a start-up cycle. ?I don't know if anyone else has had this problem, but DON'T TAKE THE CHANCE! Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com --- On Wed, 4/8/09, Andrea Grantham wrote: From: Andrea Grantham Subject: Re: [Histonet] histonet off-topic comments To: "LINDA MARGRAF" Cc: histonet@lists.utsouthwestern.edu, "MKing" Date: Wednesday, April 8, 2009, 7:18 AM Linda, This really is too much - now I have this thing on my computer maliciously put there by "aa aa" that I can not get to close. More than one person has to be removed from the list and if Bernie was removed why are there emails from him this morning? Histonet has helped me so much but I'm afraid this is the last straw. I'm going to have to unsubscribe. Sad to see such a wonderful resource go down the tubes because of a handful of inconsiderate bastards. Andi Grantham Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415? ???Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello On Apr 7, 2009, at 9:52 AM, LINDA MARGRAF wrote: > Dear Histonet members: > I have removed Bernie Taupin from the list. > Let's please get back to the topic of histology and related fields.Thanks > Linda M > Histonet administrator > >>>> MKing 4/7/2009 8:43 AM >>> > a motion is hereby offered to have the active members of histonet vote > this troll off the island, or at least request that the list moderator > do so.? this person has proven to have absolutely nothing of value to > contribute to this group, and is only intent on disrupting it. > > second? > > ----------------- > Message: 16 > Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) > From: Bernie Taupin > Subject: Re: [Histonet] Nice one Kemlo > To: histonet@lists.utsouthwestern.edu > Message-ID: <453473.65848.qm@web43516.mail.sp1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > >>> Instead of spitting your venom try to learn > > Sort of like you might consider learning correct English? I mean, its > one thing if its not your first language, but despite how many people on > here called you kindly and helpful, I find it perplexing that you feel > compelled to call me a "piece of ignorant", and TWICE, no less. > > I do not speak French. This list is not in French. Ergo, my awareness > (or lack thereof) of proper French diction has absolutely nothing at all > to do with anything... aside form providing you a vehicle to call me > names and lash out. > > If you have something to say, say it. The simple fact that you do not > like me does not give you- or anyone else- wholesale right to insult me > or call me names. > > You should be ashamed of yourself, Rene. I never expected such > antisocial behavior from you, and frankly, when you behave so > regrettably, I feel no compulsion to "learn" anything form you! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ??? Please consider the environment before printing this e-mail
> ???
> ??? > ??? This e-mail, facsimile, or letter and any files or attachments transmitted with it contains
> ??? ??? information that is confidential and privileged. This information is intended only for the use of the
> ??? ??? individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further
> > ??? ??? disclosures are prohibited without proper authorization. If you are not the intended recipient, any
> ??? ??? disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
> ??? ??? violation of federal or state law and regulations. If you have received this information in error,
> ??? ??? please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at
> ??? ??? privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all
> ??? ??? applicable privileges related to this information.
> > ???
> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gmartin <@t> marshallmedical.org Wed Apr 8 10:38:35 2009 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Wed Apr 8 10:38:39 2009 Subject: [Histonet] Re: BEWARE "aa aa" staying with a link In-Reply-To: <235835.15787.qm@web31304.mail.mud.yahoo.com> References: <235835.15787.qm@web31304.mail.mud.yahoo.com> Message-ID: <6ED9D4252F278841A0593D3D788AF24C04F8B1DA@mailsvr.MARSHMED.local> If you have clicked on the "aa aa" attachment and can't get out of the loop ... use the ALT control delete buttons pushed simultaneously and you will get to the task manager ... highlight the attachment and click the button that says "end task now" that should get you out of the loop. Gary (California) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Wednesday, April 08, 2009 8:17 AM To: LINDA MARGRAF; Andrea Grantham Cc: histonet@lists.utsouthwestern.edu; MKing Subject: [Histonet] Re: BEWARE "aa aa" staying with a link Hi,Late last night I saw the posting from??"aa aa"?regarding staying, and there was a link, which I stupidly linked on to. ?What a BIG MISTAKE! ?It is a musical video. ?I started listening to it and it was cute, then I tried to close it. It stayed on, and on, and on! ?I have a Mac Book Pro Laptop, and I couldn't even do a force quit! ?I had to remove the battery and do a start-up cycle. ?I don't know if anyone else has had this problem, but DON'T TAKE THE CHANCE! Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com --- On Wed, 4/8/09, Andrea Grantham wrote: From: Andrea Grantham Subject: Re: [Histonet] histonet off-topic comments To: "LINDA MARGRAF" Cc: histonet@lists.utsouthwestern.edu, "MKing" Date: Wednesday, April 8, 2009, 7:18 AM Linda, This really is too much - now I have this thing on my computer maliciously put there by "aa aa" that I can not get to close. More than one person has to be removed from the list and if Bernie was removed why are there emails from him this morning? Histonet has helped me so much but I'm afraid this is the last straw. I'm going to have to unsubscribe. Sad to see such a wonderful resource go down the tubes because of a handful of inconsiderate bastards. Andi Grantham Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415? ???Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello On Apr 7, 2009, at 9:52 AM, LINDA MARGRAF wrote: > Dear Histonet members: > I have removed Bernie Taupin from the list. > Let's please get back to the topic of histology and related fields.Thanks > Linda M > Histonet administrator > >>>> MKing 4/7/2009 8:43 AM >>> > a motion is hereby offered to have the active members of histonet vote > this troll off the island, or at least request that the list moderator > do so.? this person has proven to have absolutely nothing of value to > contribute to this group, and is only intent on disrupting it. > > second? > > ----------------- > Message: 16 > Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) > From: Bernie Taupin > Subject: Re: [Histonet] Nice one Kemlo > To: histonet@lists.utsouthwestern.edu > Message-ID: <453473.65848.qm@web43516.mail.sp1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > >>> Instead of spitting your venom try to learn > > Sort of like you might consider learning correct English? I mean, its > one thing if its not your first language, but despite how many people on > here called you kindly and helpful, I find it perplexing that you feel > compelled to call me a "piece of ignorant", and TWICE, no less. > > I do not speak French. This list is not in French. Ergo, my awareness > (or lack thereof) of proper French diction has absolutely nothing at all > to do with anything... aside form providing you a vehicle to call me > names and lash out. > > If you have something to say, say it. The simple fact that you do not > like me does not give you- or anyone else- wholesale right to insult me > or call me names. > > You should be ashamed of yourself, Rene. I never expected such > antisocial behavior from you, and frankly, when you behave so > regrettably, I feel no compulsion to "learn" anything form you! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ??? Please consider the environment before printing this e-mail
> ???
> ??? > ??? This e-mail, facsimile, or letter and any files or attachments transmitted with it contains
> ??? ??? information that is confidential and privileged. This information is intended only for the use of the
> ??? ??? individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further
> > ??? ??? disclosures are prohibited without proper authorization. If you are not the intended recipient, any
> ??? ??? disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
> ??? ??? violation of federal or state law and regulations. If you have received this information in error,
> ??? ??? please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at
> ??? ??? privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all
> ??? ??? applicable privileges related to this information.
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> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> uropartners.com Wed Apr 8 10:41:06 2009 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Wed Apr 8 10:41:12 2009 Subject: [Histonet] Take care openning ANY attachments or links Message-ID: Just a general word of warning. Anytime you are opening email from unknown mailers (including people on listserves) be careful about opening links and attachments. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 From mucram11 <@t> comcast.net Wed Apr 8 11:16:32 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Wed Apr 8 11:16:37 2009 Subject: [Histonet] histonet off-topic comments In-Reply-To: References: <001501c9b856$555c4b60$0014e220$@net> Message-ID: <000d01c9b865$61d07970$25716c50$@net> Unfortunately some of us don't use our work e-mail address due to rules or convenience. Pam Marcum -----Original Message----- From: Kevin Gillinder [mailto:k.r.gillinder@newcastle.ac.uk] Sent: Wednesday, April 08, 2009 12:03 PM To: Pamela Marcum; Andrea Grantham; LINDA MARGRAF Cc: histonet@lists.utsouthwestern.edu; MKing Subject: Re: [Histonet] histonet off-topic comments Easily Fixed. Remove any 'non-educational / institutional ' email addresses from the listserv...... -kevin On 08/04/2009 15:28, "Pamela Marcum" wrote: AMEN!! Andi - after a while you get tired of first reviewing every message for the good ones and then deleting everything else as fast as you can without opening them. This is too valuable a service to Histology to lose over a few malicious individuals who obviously have no interest in the field or what we do or worse may not even be histologists. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Wednesday, April 08, 2009 10:19 AM To: LINDA MARGRAF Cc: histonet@lists.utsouthwestern.edu; MKing Subject: Re: [Histonet] histonet off-topic comments Linda, This really is too much - now I have this thing on my computer maliciously put there by "aa aa" that I can not get to close. More than one person has to be removed from the list and if Bernie was removed why are there emails from him this morning? Histonet has helped me so much but I'm afraid this is the last straw. I'm going to have to unsubscribe. Sad to see such a wonderful resource go down the tubes because of a handful of inconsiderate bastards. Andi Grantham Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello On Apr 7, 2009, at 9:52 AM, LINDA MARGRAF wrote: > Dear Histonet members: > I have removed Bernie Taupin from the list. > Let's please get back to the topic of histology and related > fields.Thanks > Linda M > Histonet administrator > >>>> MKing 4/7/2009 8:43 AM >>> > a motion is hereby offered to have the active members of histonet vote > this troll off the island, or at least request that the list moderator > do so. this person has proven to have absolutely nothing of value to > contribute to this group, and is only intent on disrupting it. > > second? > > ----------------- > Message: 16 > Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) > From: Bernie Taupin > Subject: Re: [Histonet] Nice one Kemlo > To: histonet@lists.utsouthwestern.edu > Message-ID: <453473.65848.qm@web43516.mail.sp1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > >>> Instead of spitting your venom try to learn > > Sort of like you might consider learning correct English? I mean, its > one thing if its not your first language, but despite how many > people on > here called you kindly and helpful, I find it perplexing that you feel > compelled to call me a "piece of ignorant", and TWICE, no less. > > I do not speak French. This list is not in French. Ergo, my awareness > (or lack thereof) of proper French diction has absolutely nothing at > all > to do with anything... aside form providing you a vehicle to call me > names and lash out. > > If you have something to say, say it. The simple fact that you do not > like me does not give you- or anyone else- wholesale right to insult > me > or call me names. > > You should be ashamed of yourself, Rene. I never expected such > antisocial behavior from you, and frankly, when you behave so > regrettably, I feel no compulsion to "learn" anything form you! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Please consider the > environment before printing this e-mail
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> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dspears <@t> mmci.org Wed Apr 8 11:23:49 2009 From: dspears <@t> mmci.org (Dana Spears) Date: Wed Apr 8 11:24:24 2009 Subject: [Histonet] Benchmark XT Message-ID: I would definitely check your slides - we have found that when we get a bad lot of charged slides, things start washing off on the XT - regardless of detection, antibodies, whatever. It was happening to us on some tissue types and not on others, but a new batch of charged slides did the trick. I've never had to dry 60 min in the oven - 20 does it for us when we aren't having slide issues. Also, if you are using any Sta-on or any adhesive in your waterbath with the charged slides it can cause the slides to sort of repel the tissue and negate the "charged" effect.... again certain tissues will fall off, others stay on.... Dana Spears, HTL(ASCP) Laboratory Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) (309) 279-3768 (fax) dspears@mmci.org >>> Phyllis Thaxton 4/8/2009 8:43:36 AM >>> Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. From gu.lang <@t> gmx.at Wed Apr 8 11:25:27 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Apr 8 11:25:36 2009 Subject: AW: [Histonet] Benchmark XT In-Reply-To: <332647.97028.qm@web43512.mail.sp1.yahoo.com> References: <332647.97028.qm@web43512.mail.sp1.yahoo.com> Message-ID: Phyllis, we mainly don't have this problem and have been using the ultraview for at least one year. Do you airdry the slides horizontally? We only have the problem of loosing tissue, when the water under the sections was not moved away well enough. And we often start the run directly after cutting - no longer airdrying or oven. I would recommend to put the slides directly after cutting in the 60?C oven and dry vertically. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Phyllis Thaxton Gesendet: Mittwoch, 08. April 2009 15:44 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Benchmark XT Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with? needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 ?hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nestor.barrezueta <@t> bms.com Wed Apr 8 11:30:28 2009 From: nestor.barrezueta <@t> bms.com (Barrezueta, Nestor) Date: Wed Apr 8 11:30:34 2009 Subject: [Histonet] anti-parvalbumin antibody protocol Message-ID: I am looking for a protocol to detect parvalbumin in frozen sections of human brain. Antibody suggestion is also needed. Thanks, Nestor Nestor X. Barrezueta Bristol-Myers Squibb Company Applied Genomics 5 Research Parkway Wallingford, CT 06492-7660 203.677.6353 fax 203.677.3990 nestor.barrezueta@bms.com From Jacqueline.Farnsworth <@t> cls.ab.ca Wed Apr 8 11:31:26 2009 From: Jacqueline.Farnsworth <@t> cls.ab.ca (Jacqueline Farnsworth) Date: Wed Apr 8 11:31:31 2009 Subject: [Histonet] Formula 83 users? Message-ID: HI all. I have searched the Histonet archives and found a lot of very positive reviews regarding Formula 83. I am wondering if anyone has encountered any issues with using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there any miscibility issues? Are you soaking in xylene prior to tape coverslipping required? Any helpful hints/tips/tricks? Thank you in advance. Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From BoozerKA <@t> ah.org Wed Apr 8 12:26:47 2009 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Wed Apr 8 12:27:06 2009 Subject: [Histonet] Colloidal Iron control Message-ID: <49DC7BE5.4AA8.00C0.0@ah.org> What are you using? From agrobe2555 <@t> aol.com Wed Apr 8 12:30:21 2009 From: agrobe2555 <@t> aol.com (agrobe2555@aol.com) Date: Wed Apr 8 12:31:01 2009 Subject: [Histonet] Marker for Rabbit B-cells Message-ID: <8CB8686D419E53F-AAC-C00@WEBMAIL-DZ07.sysops.aol.com> Can anyone provide me with information for antibodies that will detect rabbit B-cells/plasmacytes?? This would be used for FFPE tissues. Thanks Albert Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana From jrobertson <@t> pathologysciences.com Wed Apr 8 12:33:09 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Wed Apr 8 12:33:16 2009 Subject: [Histonet] Eosinophilic granulocytes In-Reply-To: <7CEB62F1535B9E44AD8A5FEFB2E10F6E0215143F@CCHSCLEXMB56.cc.ad.cchs.net> References: <8865601DD17A554CB489C17FFD8A51B20242440C@MAIL04.tsn.tno.nl> <7CEB62F1535B9E44AD8A5FEFB2E10F6E0215143F@CCHSCLEXMB56.cc.ad.cchs.net> Message-ID: <518CD6920AA7154193CBE5977CD880733A8933@psmgsrv2.PSMG.local> Chromotrope 2R also works well. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Histology Day Supervisor 183 E. 8th Ave. Chico, CA 95926 530-891-6244 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pritchard, Michele Sent: Tuesday, April 07, 2009 7:32 AM To: Bruijntjes, J.P. (Joost); Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Eosinophilic granulocytes Good morning: I have good luck by simply using haematoxylin and eosin staining....the eosinophils pop right out by virtue of their bright pink/red granules! When used in conjunction with nuclear morphology, one can, with confidence, call these cells eosinophils. I have never performed IHC for eosinophils, but just did a quick search and found this helpful website. I am sure there are more websites like this, but this one will certainly get you started. http://www.antibodybeyond.com/reviews/cell-markers/eosinophil-marker.htm Best of luck -->mtp Michele T. Pritchard, Ph.D. Research Associate Nagy Laboratory Department of Pathobiology/NE40 Lerner Research Institute Cleveland Clinic 9500 Euclid Avenue Cleveland, OH 44195 phone: 216.444.8613 fax: 216.636.1493 email: pritchm@ccf.org Lab location: Lerner Research Institute NE4-214 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruijntjes, J.P. (Joost) Sent: Tuesday, April 07, 2009 10:14 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosinophilic granulocytes Hi Is anyone of you aware of a marker/method to stain eosinophilic granulocytes in mouse lung tissue which is formalin fixed and paraffin embedded? Thanks Joost Bruijntjes TNO Quality of Life Zeist Holland TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================================== P Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S. News & World Report (2008). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SEsparza <@t> seton.org Wed Apr 8 12:33:25 2009 From: SEsparza <@t> seton.org (Esparza, Sandra) Date: Wed Apr 8 12:33:29 2009 Subject: FW: [Histonet] Benchmark XT Message-ID: <3D79F47DC92B204F9E5D35C885DFC5CB01003CAD@AUSEX2VS1.seton.org> Sandra Sandra Esparza HT(ASCP)QIHC Dell Children's Hospital of Central Texas 4900 Mueller Boulevard Austin, Texas 78723 512-324-0000 x87061 sesparza@seton.org -----Original Message----- From: Esparza, Sandra Sent: Wednesday, April 08, 2009 12:23 PM To: 'gu.lang@gmx.at' Subject: RE: [Histonet] Benchmark XT We had this happen to us. It turned out that the reaction buffer and the EZ prep were mixed up. We emptied all the carboids and started fresh. The problem solved. Hope this helps Sandra Sandra Esparza HT(ASCP)QIHC Dell Children's Hospital of Central Texas 4900 Mueller Boulevard Austin, Texas 78723 512-324-0000 x87061 sesparza@seton.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Wednesday, April 08, 2009 11:25 AM To: 'Phyllis Thaxton' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Benchmark XT Phyllis, we mainly don't have this problem and have been using the ultraview for at least one year. Do you airdry the slides horizontally? We only have the problem of loosing tissue, when the water under the sections was not moved away well enough. And we often start the run directly after cutting - no longer airdrying or oven. I would recommend to put the slides directly after cutting in the 60?C oven and dry vertically. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Phyllis Thaxton Gesendet: Mittwoch, 08. April 2009 15:44 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Benchmark XT Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with? needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 ?hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jrobertson <@t> pathologysciences.com Wed Apr 8 12:38:39 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Wed Apr 8 12:38:45 2009 Subject: [Histonet] MAMMOGLOBIN In-Reply-To: References: <24A4826E8EF0964D86BC5317306F58A52BB259341F@mmc-mail.ad.mhsil.com> Message-ID: <518CD6920AA7154193CBE5977CD880733A8934@psmgsrv2.PSMG.local> I use the Ultra View Detection kit on the Ventana Benchmark XT with the following protocol: Deparaffinization CC1 Mild Mammaglobin - 32 min. Ultra Wash Hematoxylin - 4 min. Bluing - 4 min. We have very good results. Mammaglobin isn't expressed in every breast case either, so the control could be the culprit. We had to try a few tissues before we found one that yielded good results. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Histology Day Supervisor 183 E. 8th Ave. Chico, CA 95926 530-891-6244 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, April 07, 2009 10:36 AM To: 'Vickroy, Jim' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] MAMMOGLOBIN I tried also this antibody on Ventana Benchmark. Pity, I had no results. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Vickroy, Jim Gesendet: Dienstag, 07. April 2009 17:54 An: 'Histonet@lists.utsouthwestern.edu' Betreff: [Histonet] MAMMOGLOBIN Has anyone used the DAKO predilute antibody, Mammoglobin, on the Ventana Benchmark XT? And if so.....could you share the protocol you used? Finally looking on the Dako website it appears that they have ready to use, could you share with me which antibody should be used with the Ventana I-view detection kit? Thanks Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy.jim@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dchihc <@t> yahoo.com Wed Apr 8 12:43:10 2009 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Wed Apr 8 12:43:13 2009 Subject: [Histonet] Michael LaFriniere Message-ID: <232405.46746.qm@web43506.mail.sp1.yahoo.com> Please give me a call stranger...205-759-7487 Phyllis From gu.lang <@t> gmx.at Wed Apr 8 12:49:24 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Apr 8 12:49:34 2009 Subject: AW: [Histonet] Colloidal Iron control In-Reply-To: <49DC7BE5.4AA8.00C0.0@ah.org> References: <49DC7BE5.4AA8.00C0.0@ah.org> Message-ID: You can use tissue, that is also alcianblue-positiv. Like skin, aorta, cardilage, umbilical cord.. Renal clearcellcarcinoma (I don't know the exact english term). Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Kathleen Boozer Gesendet: Mittwoch, 08. April 2009 19:27 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Colloidal Iron control What are you using? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dchihc <@t> yahoo.com Wed Apr 8 13:01:30 2009 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Wed Apr 8 13:01:33 2009 Subject: [Histonet] Michael LaFriniere Message-ID: <203045.40727.qm@web43515.mail.sp1.yahoo.com> please call me?at work?205-759-7487 Phyllis From jflinn <@t> gmu.edu Wed Apr 8 12:21:41 2009 From: jflinn <@t> gmu.edu (Jane M Flinn) Date: Wed Apr 8 13:32:23 2009 Subject: [Histonet] Re: BEWARE "aa aa" staying with a link In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C04F8B1DA@mailsvr.MARSHMED.local> References: <235835.15787.qm@web31304.mail.mud.yahoo.com> <6ED9D4252F278841A0593D3D788AF24C04F8B1DA@mailsvr.MARSHMED.local> Message-ID: I had to turn off my PC. Luckily that killed it. jane "Life is short - make haste to be kind" Dr. Jane Flinn Director, Undergraduate Neuroscience Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: "Martin, Gary" Date: Wednesday, April 8, 2009 11:38 am Subject: RE: [Histonet] Re: BEWARE "aa aa" staying with a link > If you have clicked on the "aa aa" attachment and can't get out of > the loop ... use the ALT control delete buttons pushed > simultaneously and you will get to the task manager ... highlight > the attachment and click the button that says "end task now" that > should get you out of the loop. > Gary (California) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha > Sent: Wednesday, April 08, 2009 8:17 AM > To: LINDA MARGRAF; Andrea Grantham > Cc: histonet@lists.utsouthwestern.edu; MKing > Subject: [Histonet] Re: BEWARE "aa aa" staying with a link > > Hi,Late last night I saw the posting from??"aa aa"?regarding > staying, and there was a link, which I stupidly linked on to. > ?What a BIG MISTAKE! ?It is a musical video. ?I started listening > to it and it was cute, then I tried to close it. It stayed on, and > on, and on! ?I have a Mac Book Pro Laptop, and I couldn't even do > a force quit! ?I had to remove the battery and do a start-up > cycle. ?I don't know if anyone else has had this problem, but > DON'T TAKE THE CHANCE! > > Akemi > Akemi Allison-Tacha BS, HT (ASCP) HTL > > Histology Manager > > Associated Pathology Medical Group Laboratories > > 105A Cooper Court, Los Gatos, CA 95032 > > Cell: (425) 941-4287 > > E-Mail: akemiat3377@yahoo.com > > --- On Wed, 4/8/09, Andrea Grantham > wrote: > From: Andrea Grantham > Subject: Re: [Histonet] histonet off-topic comments > To: "LINDA MARGRAF" > Cc: histonet@lists.utsouthwestern.edu, "MKing" > Date: Wednesday, April 8, 2009, 7:18 AM > > Linda, > > This really is too much - now I have this thing on my computer > maliciously put there by "aa aa" that I can not get to close. More > than one person has to be removed from the list and if Bernie was > removed why are there emails from him this morning? Histonet has > helped me so much but I'm afraid this is the last straw. I'm going > to have to unsubscribe. Sad to see such a wonderful resource go > down the tubes because of a handful of inconsiderate bastards. > > Andi Grantham > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cell Biology and Anatomy > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415? ???Fax: 520.626.2097 > > "happy slicing and dicing and may all your stains work perfectly" - > Paula Sicurello > > > > > On Apr 7, 2009, at 9:52 AM, LINDA MARGRAF wrote: > > > Dear Histonet members: > > I have removed Bernie Taupin from the list. > > Let's please get back to the topic of histology and related > fields.Thanks> Linda M > > Histonet administrator > > > >>>> MKing 4/7/2009 8:43 AM >>> > > a motion is hereby offered to have the active members of > histonet vote > > this troll off the island, or at least request that the list > moderator> do so.? this person has proven to have absolutely > nothing of value to > > contribute to this group, and is only intent on disrupting it. > > > > second? > > > > ----------------- > > Message: 16 > > Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) > > From: Bernie Taupin > > Subject: Re: [Histonet] Nice one Kemlo > > To: histonet@lists.utsouthwestern.edu > > Message-ID: <453473.65848.qm@web43516.mail.sp1.yahoo.com> > > Content-Type: text/plain; charset=iso-8859-1 > > > >>> Instead of spitting your venom try to learn > > > > Sort of like you might consider learning correct English? I > mean, its > > one thing if its not your first language, but despite how many > people on > > here called you kindly and helpful, I find it perplexing that > you feel > > compelled to call me a "piece of ignorant", and TWICE, no less. > > > > I do not speak French. This list is not in French. Ergo, my > awareness> (or lack thereof) of proper French diction has > absolutely nothing at all > > to do with anything... aside form providing you a vehicle to > call me > > names and lash out. > > > > If you have something to say, say it. The simple fact that you > do not > > like me does not give you- or anyone else- wholesale right to > insult me > > or call me names. > > > > You should be ashamed of yourself, Rene. I never expected such > > antisocial behavior from you, and frankly, when you behave so > > regrettably, I feel no compulsion to "learn" anything form you! > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ??? Please consider the > environment before printing this e-mail
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> > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From micropathlabs <@t> yahoo.com Wed Apr 8 13:57:19 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Wed Apr 8 13:57:23 2009 Subject: [Histonet] Negative IHC controls Message-ID: <858441.67063.qm@web57806.mail.re3.yahoo.com> I have a question concerning ANP.22570 on the CAP checklist.?Could?you all tell me?how you are handling negative controls for IHC staining? The question actually states (and I've confirmed with CAP) that we should be running two types of negative controls. One for reagents and one for?each antibody in a run. I'd like to know what?the practice is. This seems very costly and time consuming. Thanks in advance! ? Sheila Haas Laboratory Supervisor Micro Path Laboratories From kbradshaw <@t> lcpath.com Wed Apr 8 14:13:19 2009 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Wed Apr 8 14:13:25 2009 Subject: [Histonet] Benchmark XT In-Reply-To: Message-ID: <20e8a532dd829543bf5bc49072095389@mail2.lcpath.com> We just recently went through a similar problem with irregular staining, light staining, sometimes no stain of any kind including counterstain,tissue falling off, and a handfull of false negative patient tissues, but positive controls (on the same slide). With two XT's running continuously it taken several days and everyone's help isolating the problem....not to mention several different lots numbers of slides....yikes! Next time I'll blame the slides right off the bat. Kari L. Bradshaw HT(ASCP) Laboratory Manager Lower Columbia Pathologists (360)425-5620 kbradshaw@lcpath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Spears Sent: Wednesday, April 08, 2009 9:24 AM To: histonet@lists.utsouthwestern.edu; Phyllis Thaxton Subject: Re: [Histonet] Benchmark XT I would definitely check your slides - we have found that when we get a bad lot of charged slides, things start washing off on the XT - regardless of detection, antibodies, whatever. It was happening to us on some tissue types and not on others, but a new batch of charged slides did the trick. I've never had to dry 60 min in the oven - 20 does it for us when we aren't having slide issues. Also, if you are using any Sta-on or any adhesive in your waterbath with the charged slides it can cause the slides to sort of repel the tissue and negate the "charged" effect.... again certain tissues will fall off, others stay on.... Dana Spears, HTL(ASCP) Laboratory Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) (309) 279-3768 (fax) dspears@mmci.org >>> Phyllis Thaxton 4/8/2009 8:43:36 AM >>> Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garret.t.miyamoto <@t> us.army.mil Wed Apr 8 14:17:17 2009 From: garret.t.miyamoto <@t> us.army.mil (Miyamoto, Garret T Mr CIV USA USAMEDCOM) Date: Wed Apr 8 14:17:28 2009 Subject: [Histonet] Re: Recycled Formalin In-Reply-To: <0KHR00MNQNXM4N10@mail23.us.army.mil> References: <0KHR00MNQNXM4N10@mail23.us.army.mil> Message-ID: ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009 7:15 pm Subject: Histonet Digest, Vol 65, Issue 17 To: histonet@lists.utsouthwestern.edu > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Large coverslips? (Va Paula Sicurello) > 2. HI Downdraft fume extractor for sale (Cathy Mayton) > 3. breaking glass jar/vials for MMA embedded specimens (Cathy > Mayton) 4. AW: [Histonet] MAMMOGLOBIN (Gudrun Lang) > 5. Re: AE1/AE3 nuclear staining (Rene J Buesa) > 6. Re: AE1/AE3 nuclear staining (Mark Tarango) > 7. RE: Tissue Capture Pen (Atoska Gentry) > 8. RE: I'm outta here (Smith, Allen) > 9. Controls needed! (Knutson, Deanne) > 10. Fixation question - Cerebellar granular cells (Guillermo > Palchik) 11. Recycled formalin (Richard Cartun) > 12. RE: Unsubscribe (Tony Henwood) > 13. RE: gi microwave processing (Tony Henwood) > 14. RE: AE1/AE3 nuclear staining (Tony Henwood) > 15. Re: Recycled formalin (Greg Dobbin) > 16. Re: Fixation question - Cerebellar granular cells (TF) > 17. Re: Staying... (Bernie Taupin) > 18. Re: Staying... (Bernie Taupin) > 19. RE: Unsubscribe (aa aa) > 20. Re: Whole human feet (Bernie Taupin) > 21. Re: "FREEZY" spray (Bernie Taupin) > 22. Re: "FREEZY" spray (Akemi Allison-Tacha) > 23. Re: "FREEZY" spray (Bernie Taupin) > > > ------------------------------------------------------------------- > --- > > Message: 1 > Date: Tue, 7 Apr 2009 10:17:43 -0700 (PDT) > From: Va Paula Sicurello < > Subject: Re: [Histonet] Large coverslips? > To: histonet@lists.utsouthwestern.edu, yvan lindekens > < > Message-ID: < > Content-Type: text/plain; charset=utf-8 > > > Hi Yvan, > > Does it have to be a coverslip? There are some mounting media that harden and form a barrier with optical qualities similar to that of glass. > > It might not be a perfect solution, but it might work. > > I think the stuff I used was called Crystal Mount (?). > > Paula :-) > > Paula Sicurello > VA Medical Center San Diego > Veterans Medical Research Foundation (VMRF) > Core Research Imaging Center > 3350 La Jolla Village Dr.., MC151 > San Diego, CA 92161 > 858-552-8585 x2397 > > > --- On Tue, 4/7/09, yvan lindekens < wrote: > > > From: yvan lindekens < > > Subject: [Histonet] Large coverslips? > > To: histonet@lists.utsouthwestern.edu > > Date: Tuesday, April 7, 2009, 9:05 AM > > > > Hi all, > > > > I???m looking for some kind of a DIY coverslip or a tape, > > plastic foil??? anything usable to cover some large slides > > (+/- 4.5cm * 15cm = 1.8inch * 5.9inch) containing botanical > > and zoological sections mounted in Canada Balsam. > > > > Does anyone has a (cheap???) solution for this? It > > doesn???t need to be pristine optical quality as the slides > > are primarely intended to be used on an overhead projector > > in the class room, but the possibility of viewing them under > > low power (40x - 100x) would be a real advantage. > > > > Thanks in advance four your wisdom and knowledge! > > > > Yvan. > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > ------------------------------ > > Message: 2 > Date: Tue, 7 Apr 2009 10:23:02 -0700 > From: "Cathy Mayton" < > Subject: [Histonet] HI Downdraft fume extractor for sale > To: < > Message-ID: < > Content-Type: text/plain; charset="iso-8859-1" > > I have a HI downdraft fume extractor for $50 and we will provide carbon to refill the drawer. > > Cathy A. Mayton > Wasatch Histo Consultants, Inc. > 775-625-4425 > > > > ------------------------------ > > Message: 3 > Date: Tue, 7 Apr 2009 10:28:44 -0700 > From: "Cathy Mayton" < > Subject: [Histonet] breaking glass jar/vials for MMA embedded > specimens > To: < > Message-ID: < > Content-Type: text/plain; charset="iso-8859-1" > > Yes, the scintillation vials are notorious for shattering in the freezer. However, if you put them in a plastic container with a lid on, the shattered glass will be contained and not all over your freezer. When breaking larger glass containers, again place them in the freezer, remove from the freezer after 20 minutes or so, take the lid off, wrap jar in paper towels and hold the ends closed. Wear glovesand eye protection in case shards of glass escape. Strike the jar with a hammer and unroll the paper towel over a garbage can. Rinse the block in tap water to remove any small shards of glass. This is a great way to vent frustration!! > > Cathy A. Mayton > Wasatch Histo Consultants, Inc. > > > > ------------------------------ > > Message: 4 > Date: Tue, 7 Apr 2009 19:36:14 +0200 > From: "Gudrun Lang" < > Subject: AW: [Histonet] MAMMOGLOBIN > To: "'Vickroy, Jim'" < > Cc: histonet@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset="iso-8859-1" > > I tried also this antibody on Ventana Benchmark. Pity, I had no results. > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Vickroy, > Jim > Gesendet: Dienstag, 07. April 2009 17:54 > An: 'Histonet@lists.utsouthwestern.edu' > Betreff: [Histonet] MAMMOGLOBIN > > > Has anyone used the DAKO predilute antibody, Mammoglobin, on the Ventana > Benchmark XT? And if so.....could you share the protocol you used? > Finally looking on the Dako website it appears that they have ready to use, > could you share with me which antibody should be used with the Ventana > I-view detection kit? > > Thanks > > > > > Jim Vickroy BS, HT(ASCP) > Technical Supervisor - Surgical and Autopsy Pathology > Memorial Medical Center > 217-788-4046 > vickroy.jim@mhsil.com > > > > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 5 > Date: Tue, 7 Apr 2009 12:16:44 -0700 (PDT) > From: Rene J Buesa < > Subject: Re: [Histonet] AE1/AE3 nuclear staining > To: histonet@lists.utsouthwestern.edu, Tammy Barnhart > < > Message-ID: < > Content-Type: text/plain; charset=iso-8859-1 > > Have you changed any of the reagents in your X-press tissue processor or other aspect of your tissue processing protocol? This could be?a factor. > Ren? J. > > --- On Tue, 4/7/09, Tammy Barnhart < wrote: > > From: Tammy Barnhart < > Subject: [Histonet] AE1/AE3 nuclear staining > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, April 7, 2009, 12:53 PM > > We have recently been having dark nuclear staining with our AE1/AE3 > antibody. This is a recent event and we cannot figure out what is going > on. It started gradually and has been increasing in intensity. We have > not changed antibody lots or any other part of our protocol. Has anyone > seen this before? Any suggestions on what is happening here? > > Tammy Barnhart, BS, HTL(ASCP) > > Avera McKennan Hospital > > > > ----------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is for > the sole use of the intended recipient(s) and may contain confidential and > privileged information. Any unauthorized review, use, disclosure, or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 6 > Date: Tue, 7 Apr 2009 12:22:13 -0700 > From: Mark Tarango < > Subject: Re: [Histonet] AE1/AE3 nuclear staining > To: Tammy Barnhart < > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > < > Content-Type: text/plain; charset=ISO-8859-1 > > When was the last time you checked the pH of your retrieval solution? Or > are you using an enzyme? > > Mark Tarango > > On Tue, Apr 7, 2009 at 9:53 AM, Tammy Barnhart > > > We have recently been having dark nuclear staining with our AE1/AE3 > > antibody. This is a recent event and we cannot figure out what is going > > on. It started gradually and has been increasing in intensity. We have > > not changed antibody lots or any other part of our protocol. Has anyone > > seen this before? Any suggestions on what is happening here? > > > > Tammy Barnhart, BS, HTL(ASCP) > > > > Avera McKennan Hospital > > > > > > > > ----------------------------------------- > > Confidentiality Notice: This e-mail message, including any attachments, is > > for the sole use of the intended recipient(s) and may contain confidential > > and privileged information. Any unauthorized review, use, disclosure, or > > distribution is prohibited. If you are not the intended recipient, please > > contact the sender by reply e-mail and destroy all copies of the original > > message. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 7 > Date: Tue, 07 Apr 2009 14:28:16 -0500 > From: Atoska Gentry < > Subject: [Histonet] RE: Tissue Capture Pen > To: Histonet < > Message-ID: < > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > hello, if any of you are using and/or have used the Ted Pella, Inc. > Tissue Capture Pen will you be so kind as to share with me your > experience (s), pros, cons and whatever else you deem necessary? Also, > does it's use require special glass slides? Any info you can provide on > it's use ASAP will be much appreciated. Thank you kindly, Atoska > > > > ------------------------------ > > Message: 8 > Date: Tue, 7 Apr 2009 15:40:35 -0400 > From: "Smith, Allen" < > Subject: [Histonet] RE: I'm outta here > To: "'Histonet@lists.utsouthwestern.edu'" > < > Message-ID: > < > > Content-Type: text/plain; charset="us-ascii" > > I can spot spam and o.t. posts and delete them in a half second each. The questions on Histonet make me aware of the extent of use of special stains, and the potential for new stains. Jjob postings are forwarded to the career service office here. The safety warnings are often very useful. HIstonet is a great source of information on antigen retrieval techniques and antibodies. I would really miss Histonet. > --Allen A Smith, Ph.D. > Barry University School of Podiatric Medicine > > > ------------------------------ > > Message: 9 > Date: Tue, 7 Apr 2009 15:06:13 -0500 > From: "Knutson, Deanne" < > Subject: [Histonet] Controls needed! > To: "'histonet@lists.utsouthwestern.edu'" > < > Message-ID: < > Content-Type: text/plain > > We are looking for H. Pylori control tissue and also GMS/fungus control > tissue. Is there anyone out there that might have extra to share? We have > good GRAM control blocks that we would be happy to exchange. Please let me > know if you can help us out. Thank you! > > > > Deanne Knutson > > Anatomic Pathology Supervisor > > St. Alexius Medical Center > > Bismarck, N. Dak. 58506 > > 701-530-6730 > > Fax 701-530-6735 > > > > ------------------------------ > > Message: 10 > Date: Tue, 7 Apr 2009 16:59:58 -0400 > From: Guillermo Palchik < > Subject: [Histonet] Fixation question - Cerebellar granular cells > To: histonet@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes > > Dear Histoneters, > > I am doing some IHC on rat cerebellar granular cells (NOT TISSUE) and > I need to fix them with PF. > I have gotten 4 different answers on how to go about this, and I > wanted to run this by the list to see what you think. > > 1) Fix in COLD, 4% PF in 1x PBS > > 2) Fix in COLD, 4% PF in 1x PBS with 4% Sucrose. > > 3) Fix in WARM (37 C) ,in 4% PF in 1x PBS. > > 4) Fix in WARM (37 C), in 1x PBS with 4% Sucrose. > > I get the fact that the PF might need to be at 37 C, since it is the > temperature that the cells are in the incubator and it would probably > temperature shock them. What about the sucrose? does it remove the > water? > > I'd appreciate your thoughts... > Best, > > Guillermo > > Guillermo Palchik > gp62@georgetown.edu > > > > > > > > > > ------------------------------ > Richard, We do use recycled formalin for our surgical and autopsy tissues. We have a "Procycler" from B/R Instrument Corporation that recycles used formalin. It comes with a kit to test the recycled product and also a "buffering solution" to add to it. We have no problems with fixation using recycled formalin. Garret Miyamoto Tripler Army Medical Center > Message: 11 > Date: Tue, 07 Apr 2009 18:30:04 -0400 > From: "Richard Cartun" < > Subject: [Histonet] Recycled formalin > To: "Histonet" < > Message-ID: < > Content-Type: text/plain; charset=US-ASCII > > Is anyone using recycled formalin for primary fixation of either surgical or autopsy tissue? Thanks. > > Richard > > Richard W. Cartun, Ph.D. > Director, Histology & Immunopathology > Director, Biospecimens > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > > > > ------------------------------ > > Message: 12 > Date: Wed, 8 Apr 2009 08:44:12 +1000 > From: "Tony Henwood" < > Subject: RE: [Histonet] Unsubscribe > To: <, < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Sophie, > I for one would never throw a torrent of abuse at any one (mmm unless > they are politicians - in between football seasons!) > > So if my (and most others on Histonet) comments are not of some worth > then we apologise. > > We need to try harder. > > (also please remember the delete key, I unfortunately have to regularly > use it) > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > S.J.Ainsworth@bsms.ac.uk > Sent: Tuesday, 7 April 2009 5:53 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Unsubscribe > > > Please can you unsubscribe me from this mailing list. As an > inexperienced histologist trying to find my feet I no longer feel like I > could ask any questions as I'm sure I would get a torrent of abuse back > instead of any helpful comments. > > > > Sophie Ainsworth > Brighton and Sussex Medical School > Medical Research Building > Falmer > East Sussex > BN1 9PX > > Tel: #44 1273 877886 > Fax: #44 1273 877884 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > > > > > ------------------------------ > > Message: 13 > Date: Wed, 8 Apr 2009 08:48:53 +1000 > From: "Tony Henwood" < > Subject: RE: [Histonet] gi microwave processing > To: <, < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Nancy, > > Best advice I can offer is to ensure as much fixation as you can. > We start with 30 minutes at 30oC and the ramp it up to 40oC for 30-60 > minutes, rinse in 70% ethanol (5min)then continue microwave processing > with isopropanol and wax. You may also consider decreasing the > processing temperatures. > > GI biopsies being quite small do not need over-the-top processing, but > in my experience, fixation is still the key. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > MAGODLEY@aol.com > Sent: Tuesday, 7 April 2009 10:11 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] gi microwave processing > > > I am starting a gi lab and using a microwave processor for my first > time. > Any suggestions? SHUR Wave processor. Thanks, Nancy > **************A Good Credit Score is 700 or Above. See yours in just 2 > easy > steps! > (http://pr.atwola.com/promoclk/100126575x1221621488x1201450096/aol?redir > =http:%2F%2Fwww.freecreditreport.com%2Fpm%2Fdefault.aspx%3Fsc%3D668072%2 > 6hmpgID > %3D62%26bcd%3DAprilfooterNO62) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > > > > > ------------------------------ > > Message: 14 > Date: Wed, 8 Apr 2009 10:06:52 +1000 > From: "Tony Henwood" < > Subject: RE: [Histonet] AE1/AE3 nuclear staining > To: "Tammy Barnhart" <, > < > Message-ID: < > Content-Type: text/plain; charset="us-ascii" > > Possibly the concentration of hydrogen peroxide in the DAB solution has > been slowly increasing (maybe micropipette creep?) > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tammy > Barnhart > Sent: Wednesday, 8 April 2009 2:54 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] AE1/AE3 nuclear staining > > > We have recently been having dark nuclear staining with our AE1/AE3 > antibody. This is a recent event and we cannot figure out what is going > on. It started gradually and has been increasing in intensity. We have > not changed antibody lots or any other part of our protocol. Has anyone > seen this before? Any suggestions on what is happening here? > > Tammy Barnhart, BS, HTL(ASCP) > > Avera McKennan Hospital > > > > ----------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are not the intended > recipient, please contact the sender by reply e-mail and destroy all > copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > > > > > ------------------------------ > > Message: 15 > Date: Tue, 07 Apr 2009 21:55:26 -0300 > From: "Greg Dobbin" < > Subject: Re: [Histonet] Recycled formalin > To: <,< > Message-ID: < > Content-Type: text/plain; charset=US-ASCII > > Yes. ?? > Greg > > Greg Dobbin, R.T. > Chief Technologist, Anatomic Pathology > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > "I find that the harder I work, the > more luck I seem to have." > - Thomas Jefferson > > >>> "Richard Cartun" < 04/07/09 7:30 PM >>> > Is anyone using recycled formalin for primary fixation of either > surgical or autopsy tissue? Thanks. > > Richard > > Richard W. Cartun, Ph.D. > Director, Histology & Immunopathology > Director, Biospecimens > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------- > Statement of Confidentiality > This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. > > D?claration de confidentialit? > Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. > ------------------------- > > > > > ------------------------------ > > Message: 16 > Date: Wed, 8 Apr 2009 11:07:21 +0800 > From: "TF" < > Subject: Re: [Histonet] Fixation question - Cerebellar granular cells > To: "Guillermo Palchik" <, "histonet" > < > Message-ID: < > Content-Type: text/plain; charset="gb2312" > > Hi, i normally work on tissue. > I think 4% PFA in 0.1M PB is fine. so approach 3. > > > 2009-04-08 > > > > TF > > > > ???????? Guillermo Palchik > ?????????? 2009-04-08 05:03:13 > ???????? histonet > ?????? > ?????? [Histonet] Fixation question - Cerebellar granular cells > > Dear Histoneters, > I am doing some IHC on rat cerebellar granular cells (NOT TISSUE) and > I need to fix them with PF. > I have gotten 4 different answers on how to go about this, and I > wanted to run this by the list to see what you think. > 1) Fix in COLD, 4% PF in 1x PBS > 2) Fix in COLD, 4% PF in 1x PBS with 4% Sucrose. > 3) Fix in WARM (37 C) ,in 4% PF in 1x PBS. > 4) Fix in WARM (37 C), in 1x PBS with 4% Sucrose. > I get the fact that the PF might need to be at 37 C, since it is the > temperature that the cells are in the incubator and it would probably > temperature shock them. What about the sucrose? does it remove the > water? > I'd appreciate your thoughts... > Best, > Guillermo > Guillermo Palchik > gp62@georgetown.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 17 > Date: Tue, 7 Apr 2009 20:40:32 -0700 (PDT) > From: Bernie Taupin < > Subject: Re: [Histonet] Staying... > To: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" <, Marla > Thomas <, histonet@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset=us-ascii > > Yes, Well-done to all of you who have decided to stay! It really separates the wheat from the chaff, as it were. Or the weenies from the real adults, more or less. And even the French-speakers from the non-French-speakers, in the case of Kemlo/Rene. Ha ha. That was a joke. Learn to laugh a little, folks. > > I, myself, have decided to stay. > > I'll try to play more nicely. Here's hoping the rest of you can be so cool about it all. > > SO BIG UPS TO ALL THE HISTOLOGISTS IN THE HOUSE! CAN I GET A WHUT-WHUT! > > > > > ________________________________ > From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" < > To: Marla Thomas <; histonet@lists.utsouthwestern.edu > Sent: Tuesday, April 7, 2009 8:10:22 AM > Subject: RE: [Histonet] Staying... > > Amen! > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marla > Thomas > Sent: Tuesday, April 07, 2009 7:48 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Staying... > > I am staying on the list-serve. I have learned a lot from people on > this list-serve. I have been in the field since 1970 and I too am still > learning things. No one has all of the answers, and sometimes there > isn't just one answer. > > I am staying on the list-serve, and if my input can help one person, or > 15 different responses can help me, then the list-serve works. There > are still people out there asking questions in between the bickering. > > Marla Thomas, HT(ASCP) > Litton Pathology Associates, PC > 700 NW Hunter Dr. > Blue Springs, MO 64015 > Phone: 816-229-6449 Fax:816-874-4400 > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Litton Pathology > Associates, P.C. and are intended only for the addressee. The > information contained in this message is confidential and may contain > privileged, confidential, proprietary and/or exemption from disclosure > under applicable law. Unauthorized forwarding, printing, copying, > distribution, or use of such information is strictly prohibited and may > be unlawful. If you are not the addressee, please promptly delete this > message and notify the sender of the delivery error by e-mail or you may > call 816-229-6449 and ask for the HIPAA/Compliance Coordinator. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 18 > Date: Tue, 7 Apr 2009 20:40:32 -0700 (PDT) > From: Bernie Taupin < > Subject: Re: [Histonet] Staying... > To: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" <, Marla > Thomas <, histonet@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset=us-ascii > > Yes, Well-done to all of you who have decided to stay! It really separates the wheat from the chaff, as it were. Or the weenies from the real adults, more or less. And even the French-speakers from the non-French-speakers, in the case of Kemlo/Rene. Ha ha. That was a joke. Learn to laugh a little, folks. > > I, myself, have decided to stay. > > I'll try to play more nicely. Here's hoping the rest of you can be so cool about it all. > > SO BIG UPS TO ALL THE HISTOLOGISTS IN THE HOUSE! CAN I GET A WHUT-WHUT! > > > > > ________________________________ > From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" < > To: Marla Thomas <; histonet@lists.utsouthwestern.edu > Sent: Tuesday, April 7, 2009 8:10:22 AM > Subject: RE: [Histonet] Staying... > > Amen! > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marla > Thomas > Sent: Tuesday, April 07, 2009 7:48 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Staying... > > I am staying on the list-serve. I have learned a lot from people on > this list-serve. I have been in the field since 1970 and I too am still > learning things. No one has all of the answers, and sometimes there > isn't just one answer. > > I am staying on the list-serve, and if my input can help one person, or > 15 different responses can help me, then the list-serve works. There > are still people out there asking questions in between the bickering. > > Marla Thomas, HT(ASCP) > Litton Pathology Associates, PC > 700 NW Hunter Dr. > Blue Springs, MO 64015 > Phone: 816-229-6449 Fax:816-874-4400 > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Litton Pathology > Associates, P.C. and are intended only for the addressee. The > information contained in this message is confidential and may contain > privileged, confidential, proprietary and/or exemption from disclosure > under applicable law. Unauthorized forwarding, printing, copying, > distribution, or use of such information is strictly prohibited and may > be unlawful. If you are not the addressee, please promptly delete this > message and notify the sender of the delivery error by e-mail or you may > call 816-229-6449 and ask for the HIPAA/Compliance Coordinator. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 19 > Date: Tue, 7 Apr 2009 20:56:34 -0700 (PDT) > From: aa aa < > Subject: RE: [Histonet] Unsubscribe > To: S.J.Ainsworth@bsms.ac.uk, histonet@lists.utsouthwestern.edu, Tony > Henwood < > Message-ID: < > Content-Type: text/plain; charset=us-ascii > > Dear Histonetters: > > > It sure is a real shame to see so many familiar names leave the list > just due to a little annoyance. Sorry to send yet *another* note about > all of this to the whole list, but I wanted to pass on a link my > supervisor sent to me when we were having similar real-life crisis in > my lab... it really does put things into perfect perspective: > http://smouch.net/lol/ > > > > Have a great day everyone, and keep HistoNetting! > > > > ~L. > > > > --- On Tue, 4/7/09, Tony Henwood < wrote: > From: Tony Henwood < > Subject: RE: [Histonet] Unsubscribe > To: S.J.Ainsworth@bsms.ac.uk, histonet@lists.utsouthwestern.edu > Date: Tuesday, April 7, 2009, 6:44 PM > > Sophie, > I for one would never throw a torrent of abuse at any one (mmm unless > they are politicians - in between football seasons!) > > So if my (and most others on Histonet) comments are not of some worth > then we apologise. > > We need to try harder. > > (also please remember the delete key, I unfortunately have to regularly > use it) > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > S.J.Ainsworth@bsms.ac.uk > Sent: Tuesday, 7 April 2009 5:53 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Unsubscribe > > > Please can you unsubscribe me from this mailing list. As an > inexperienced histologist trying to find my feet I no longer feel like I > could ask any questions as I'm sure I would get a torrent of abuse back > instead of any helpful comments. > > > > Sophie Ainsworth > Brighton and Sussex Medical School > Medical Research Building > Falmer > East Sussex > BN1 9PX > > Tel: #44 1273 877886 > Fax: #44 1273 877884 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. If > you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual > sender, and are not necessarily the views of The Children's Hospital at > Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens > Hospital at Westmead accepts no liability for any consequential damage resulting > from email containing computer viruses. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 20 > Date: Tue, 7 Apr 2009 21:07:13 -0700 (PDT) > From: Bernie Taupin < > Subject: Re: [Histonet] Whole human feet > To: Yak-Nam Wang <, > histonet@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset=us-ascii > > No offense, dude, but GROSS. > > That's probably why nobody has bitten yet, in regards to this query. > > > > > ________________________________ > From: Yak-Nam Wang < > To: histonet@lists.utsouthwestern.edu > Sent: Monday, April 6, 2009 8:04:54 PM > Subject: [Histonet] Whole human feet > > Hello all, > > If possible, we would like to obtain histological sections of adult human > feet (plane of the anterior-posterior surface). Does anyone know of > labs/groups that have done this or something similar? > > Thank you for your help > Yak-Nam Wang > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 21 > Date: Tue, 7 Apr 2009 21:06:44 -0700 (PDT) > From: Bernie Taupin < > Subject: Re: [Histonet] "FREEZY" spray > To: Jennifer MacDonald <, Ingles Claire > < > Cc: Histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset=us-ascii > > > Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it! > > I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? > > I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen. > > kisses, flowers and rainbows, > Bernie Taupin, King of Cryomicrotomy, Esq. > > > > > > ------------------------------ > > Message: 22 > Date: Tue, 7 Apr 2009 22:03:04 -0700 (PDT) > From: Akemi Allison-Tacha < > Subject: Re: [Histonet] "FREEZY" spray > To: Jennifer MacDonald <, Ingles Claire > <, Bernie Taupin < > Cc: Histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset=iso-8859-1 > > Bernie, > I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. ? > Years later, I developed the "MATSSE (pH3.4) 1-Step Trichrome Stain Kit" (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. ?This kit has long since discontinued by the way...This came from Biocare's Data Sheet. > > > NOTE: Muscle tissue should be suitably obtained and > frozen within 30 minutes after excision.? The usual > method of freezing is to first mount a transverse section of the muscle on a > chuck using 10% tragacanth gum or equivalent commercial frozen section mounting > media as the adhesive.? The chuck > with the mounted specimen is then immersed in prechilled isopentane until frozen.? Isopentane is precooled when a container of the substance is > placed into liquid nitrogen.? At a > temperature of -160? C, the isopentane has a slightly syrupy > consistency.? Care should be > taken to remove the muscle sample when freezing is complete, usually after 25 > to 30 seconds.? Too short a > freezing time produces artifacts; prolonged freezing can produce cracking of > the block. > > ? > > NOTE:?If > the sample cannot be sectioned immediately after freezing, it may be wrapped in > foil and placed in a plastic-lidded container along with a small amount of ice > for moisture.? Specimens may be > stored at -70? > C until sectioned.? > > > > > > Regards,Akemi > Akemi Allison-Tacha BS, HT (ASCP) HTL > > Histology Manager > > Associated Pathology Medical Group Laboratories > > 105A Cooper Court, Los Gatos, CA 95032 > > Cell: (425) 941-4287 > > E-Mail: akemiat3377@yahoo.com > > --- On Tue, 4/7/09, Bernie Taupin < wrote: > > From: Bernie Taupin < > Subject: Re: [Histonet] "FREEZY" spray > To: "Jennifer MacDonald" <, "Ingles Claire" < > Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu > Date: Tuesday, April 7, 2009, 9:06 PM > > > Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it! > > I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? > > I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen. > > kisses, flowers and rainbows, > Bernie Taupin, King of Cryomicrotomy, Esq. > > > > ? ? ? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 23 > Date: Tue, 7 Apr 2009 22:11:53 -0700 (PDT) > From: Bernie Taupin < > Subject: Re: [Histonet] "FREEZY" spray > To: Akemi Allison-Tacha <, Jennifer MacDonald > <, Ingles Claire < > Cc: Histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: < > Content-Type: text/plain; charset=iso-8859-1 > > I use isopentane suspended in liquid nitrogen, too. > > sorry if this sounds curt, but due to your cut-and-pasting, im not entirely sure what point youre trying to get at...? > > > > > ________________________________ > From: Akemi Allison-Tacha < > To: Jennifer MacDonald <; Ingles Claire <; Bernie Taupin < > Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > Sent: Wednesday, April 8, 2009 1:03:04 AM > Subject: Re: [Histonet] "FREEZY" spray > > > Bernie, > > I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. > > Years later, I developed the "MATSSE (pH3.4) 1-Step Trichrome Stain Kit" (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. This kit has long since discontinued by the way....This came from Biocare's Data Sheet. > > NOTE:Muscle tissue should be suitably obtained and > frozen within 30 minutes after excision. The usual > method of freezing is to first mount a transverse section of the muscle on a > chuck using 10% tragacanth gum or equivalent commercial frozen section mounting > media as the adhesive. The chuck > with the mounted specimen is then immersed in prechilled isopentane until frozen. Isopentane is precooled when a container of the substance is > placed into liquid nitrogen. At a > temperature of -160? C, the isopentane has a slightly syrupy > consistency. Care should be > taken to remove the muscle sample when freezing is complete, usually after 25 > to 30 seconds. Too short a > freezing time produces artifacts; prolonged freezing can produce cracking of > the block. > > NOTE: If > the sample cannot be sectioned immediately after freezing, it may be wrapped in > foil and placed in a plastic-lidded container along with a small amount of ice > for moisture. Specimens may be > stored at -70? C until sectioned. > Regards, > Akemi > > Akemi Allison-Tacha BS, HT (ASCP) HTL > Histology Manager > Associated Pathology Medical Group Laboratories > 105A Cooper Court, Los Gatos, CA 95032 > Cell: (425) 941-4287 > E-Mail: akemiat3377@yahoo.com > > --- On Tue, 4/7/09, Bernie Taupin < wrote: > > > From: Bernie Taupin < > Subject: Re: [Histonet] "FREEZY" spray > To: "Jennifer MacDonald" <, "Ingles Claire" < > Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu > Date: Tuesday, April 7, 2009, 9:06 PM > > > > Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it! > > I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? > > I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen. > > kisses, flowers and rainbows, > Bernie Taupin, King of Cryomicrotomy, Esq. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern..edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 65, Issue 17 > **************************************** From pbaldwin <@t> theadvisoryboardprogram.com Wed Apr 8 14:33:41 2009 From: pbaldwin <@t> theadvisoryboardprogram.com (Peter Baldwin) Date: Wed Apr 8 14:34:16 2009 Subject: [Histonet] Formula 83 users? Message-ID: I can't speak to its functionality, but per its MSDS it is extremely flammable (FP= 45F) and would, thus be considered a hazardous waste by the EPA. EPA's regulations for all "solid" hazardous waste (which includes liquids) produced by healthcare facilities and others require that they be handled (including monitoring, storage, and disposal) in accordance with EPA's requirements, and specifically state that hazardous wastes generated in the (healthcare) laboratory "cannot be disposed into drains." Since Micro-ClearT is not classified as hazardous by EPA, it is drain-disposable under its regulations. ? ?Peter Peter G. Baldwin Director of Sales, Marketing & Business Development pbaldwin@MicronEnvironmental.com Micron Environmental Industries, Inc. Green Chemistry for LifeSM www.MicronEnvironmental.com 1221 Cameron Street Alexandria, VA 22314 703-548-2776 703-548-7988/Fax From sjchtascp <@t> yahoo.com Wed Apr 8 15:23:15 2009 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Wed Apr 8 15:23:21 2009 Subject: [Histonet] Looking for "WORK" Message-ID: <210131.62501.qm@web38201.mail.mud.yahoo.com> From akemiat3377 <@t> yahoo.com Wed Apr 8 15:59:55 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Apr 8 16:00:00 2009 Subject: [Histonet] Virus planted ? BEWARE "aa aa" staying with a link Message-ID: <386164.17176.qm@web31302.mail.mud.yahoo.com> Hi All, I guess I was stupid to link on this file. ?I just hope "aa aa" didn't do this as an act of?malicious?behavior, and now we have viruses in our computers! ?I usually delete any unknown sender, and fortunately my MAC filters out most spams. ?Now I will have to do a diagnostic check. ?I recommend any of you who foolishly opened the link to do the same. Regards,Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com --- On Wed, 4/8/09, Jane M Flinn wrote: From: Jane M Flinn Subject: Re: RE: [Histonet] Re: BEWARE "aa aa" staying with a link To: "Martin, Gary" Cc: "Akemi Allison-Tacha" , "LINDA MARGRAF" , "Andrea Grantham" , histonet@lists.utsouthwestern.edu, "MKing" Date: Wednesday, April 8, 2009, 10:21 AM I had to turn off my PC. Luckily that killed it. jane "Life is short - make haste to be kind" Dr. Jane Flinn Director, Undergraduate Neuroscience Program George Mason University, 3F5 4400 University Dr. Fairfax, VA? 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: "Martin, Gary" Date: Wednesday, April 8, 2009 11:38 am Subject: RE: [Histonet] Re: BEWARE "aa aa" staying with a link > If you have clicked on the "aa aa" attachment and can't get out of > the loop ... use the ALT control delete buttons pushed > simultaneously and you will get to the task manager ... highlight > the attachment and click the button that says "end task now" that > should get you out of the loop. > Gary (California) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- > bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha > Sent: Wednesday, April 08, 2009 8:17 AM > To: LINDA MARGRAF; Andrea Grantham > Cc: histonet@lists.utsouthwestern.edu; MKing > Subject: [Histonet] Re: BEWARE "aa aa" staying with a link > > Hi,Late last night I saw the posting from??"aa aa"?regarding > staying, and there was a link, which I stupidly linked on to. > ?What a BIG MISTAKE! ?It is a musical video. ?I started listening > to it and it was cute, then I tried to close it. It stayed on, and > on, and on! ?I have a Mac Book Pro Laptop, and I couldn't even do > a force quit! ?I had to remove the battery and do a start-up > cycle. ?I don't know if anyone else has had this problem, but > DON'T TAKE THE CHANCE! > > Akemi > Akemi Allison-Tacha BS, HT (ASCP) HTL > > Histology Manager > > Associated Pathology Medical Group Laboratories > > 105A Cooper Court, Los Gatos, CA 95032 > > Cell: (425) 941-4287 > > E-Mail: akemiat3377@yahoo.com > > --- On Wed, 4/8/09, Andrea Grantham > wrote: > From: Andrea Grantham > Subject: Re: [Histonet] histonet off-topic comments > To: "LINDA MARGRAF" > Cc: histonet@lists.utsouthwestern.edu, "MKing" > Date: Wednesday, April 8, 2009, 7:18 AM > > Linda, > > This really is too much - now I have this thing on my computer > maliciously put there by "aa aa" that I can not get to close. More > than one person has to be removed from the list and if Bernie was > removed why are there emails from him this morning? Histonet has > helped me so much but I'm afraid this is the last straw. I'm going > to have to unsubscribe. Sad to see such a wonderful resource go > down the tubes because of a handful of inconsiderate bastards. > > Andi Grantham > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cell Biology and Anatomy > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415? ???Fax: 520.626.2097 > > "happy slicing and dicing and may all your stains work perfectly" - > Paula Sicurello > > > > > On Apr 7, 2009, at 9:52 AM, LINDA MARGRAF wrote: > > > Dear Histonet members: > > I have removed Bernie Taupin from the list. > > Let's please get back to the topic of histology and related > fields.Thanks> Linda M > > Histonet administrator > > > >>>> MKing 4/7/2009 8:43 AM >>> > > a motion is hereby offered to have the active members of > histonet vote > > this troll off the island, or at least request that the list > moderator> do so.? this person has proven to have absolutely > nothing of value to > > contribute to this group, and is only intent on disrupting it. > > > > second? > > > > ----------------- > > Message: 16 > > Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) > > From: Bernie Taupin > > Subject: Re: [Histonet] Nice one Kemlo > > To: histonet@lists.utsouthwestern.edu > > Message-ID: <453473.65848.qm@web43516.mail.sp1.yahoo.com> > > Content-Type: text/plain; charset=iso-8859-1 > > > >>> Instead of spitting your venom try to learn > > > > Sort of like you might consider learning correct English? I > mean, its > > one thing if its not your first language, but despite how many > people on > > here called you kindly and helpful, I find it perplexing that > you feel > > compelled to call me a "piece of ignorant", and TWICE, no less. > > > > I do not speak French. This list is not in French. Ergo, my > awareness> (or lack thereof) of proper French diction has > absolutely nothing at all > > to do with anything... aside form providing you a vehicle to > call me > > names and lash out. > > > > If you have something to say, say it. The simple fact that you > do not > > like me does not give you- or anyone else- wholesale right to > insult me > > or call me names. > > > > You should be ashamed of yourself, Rene. I never expected such > > antisocial behavior from you, and frankly, when you behave so > > regrettably, I feel no compulsion to "learn" anything form you! > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ??? Please consider the > environment before printing this e-mail
> > ???
> > ??? > > ??? This e-mail, facsimile, or > letter and any files or attachments transmitted with it > contains
> > ??? ??? information that is confidential and privileged. This > information is intended only for the use of the
> > ??? ??? individual(s) and entity(ies) to whom it is addressed. > If you are the intended recipient, further
> > > > ??? ??? disclosures are prohibited without proper authorization. > If you are not the intended recipient, any
> > ??? ??? disclosure, copying, printing, or use of this > information is strictly prohibited and possibly a
> > ??? ??? violation of federal or state law and regulations. If > you have received this information in error,
> > ??? ??? please notify Children's Medical Center Dallas > immediately at 214-456-4444 or via e-mail at
> > ??? ??? privacy@childrens.com. Children's Medical Center Dallas > and its affiliates hereby claim all
> > ??? ??? applicable privileges related to this > information.
> > > > ???
> > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jnocito <@t> satx.rr.com Wed Apr 8 16:08:02 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Apr 8 16:08:16 2009 Subject: [Histonet] Benchmark XT In-Reply-To: <20e8a532dd829543bf5bc49072095389@mail2.lcpath.com> References: <20e8a532dd829543bf5bc49072095389@mail2.lcpath.com> Message-ID: <6C14869D8BA84515848C2072D84F65A5@JoePC> another issue could be the mixer blowing too hard. I've had that happen too. JTT ----- Original Message ----- From: "Kari Bradshaw" To: "Dana Spears" ; ; "Phyllis Thaxton" Sent: Wednesday, April 08, 2009 2:13 PM Subject: RE: [Histonet] Benchmark XT We just recently went through a similar problem with irregular staining, light staining, sometimes no stain of any kind including counterstain,tissue falling off, and a handfull of false negative patient tissues, but positive controls (on the same slide). With two XT's running continuously it taken several days and everyone's help isolating the problem....not to mention several different lots numbers of slides....yikes! Next time I'll blame the slides right off the bat. Kari L. Bradshaw HT(ASCP) Laboratory Manager Lower Columbia Pathologists (360)425-5620 kbradshaw@lcpath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Spears Sent: Wednesday, April 08, 2009 9:24 AM To: histonet@lists.utsouthwestern.edu; Phyllis Thaxton Subject: Re: [Histonet] Benchmark XT I would definitely check your slides - we have found that when we get a bad lot of charged slides, things start washing off on the XT - regardless of detection, antibodies, whatever. It was happening to us on some tissue types and not on others, but a new batch of charged slides did the trick. I've never had to dry 60 min in the oven - 20 does it for us when we aren't having slide issues. Also, if you are using any Sta-on or any adhesive in your waterbath with the charged slides it can cause the slides to sort of repel the tissue and negate the "charged" effect.... again certain tissues will fall off, others stay on.... Dana Spears, HTL(ASCP) Laboratory Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) (309) 279-3768 (fax) dspears@mmci.org >>> Phyllis Thaxton 4/8/2009 8:43:36 AM >>> Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Apr 8 16:18:27 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Apr 8 16:18:39 2009 Subject: [Histonet] Negative IHC controls In-Reply-To: <858441.67063.qm@web57806.mail.re3.yahoo.com> References: <858441.67063.qm@web57806.mail.re3.yahoo.com> Message-ID: <10BF5ECBF8E44391A4F116B7C8F716D1@JoePC> we're running one negative normal serum slide per block. For example, if we're performing mouse antibodies i.e. CD-45, HMB-45, pan Cytokeratin, we run one IgG mouse normal serum control. If we are running rabbit polyclonals (or rabbit monoclonals) and mouse monoclonals on the same case, we usually would run a IgG normal mouse and normal rabbit serum slide. Now, I know the purists out there are going to tell me what if I use an IgM mouse monoclonal, I should be running an IgM mouse normal serum. I agree, but my budget doesn't allow to run a negative for all scenarios. In that frame of mind, if we run a case with multiple different pretreatments, we run a negative on the harshest pretreatment. Again, not ideal, but I think it is better than just putting PBS or TBS buffer on a slide and calling that negative. That's my 3 cents worth and I'm sticking to that story. JTT ----- Original Message ----- From: "Sheila Haas" To: Sent: Wednesday, April 08, 2009 1:57 PM Subject: [Histonet] Negative IHC controls I have a question concerning ANP.22570 on the CAP checklist. Could you all tell me how you are handling negative controls for IHC staining? The question actually states (and I've confirmed with CAP) that we should be running two types of negative controls. One for reagents and one for each antibody in a run. I'd like to know what the practice is. This seems very costly and time consuming. Thanks in advance! Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cathy <@t> wasatchhisto.com Wed Apr 8 16:45:04 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Wed Apr 8 16:45:26 2009 Subject: [Histonet] hand held pH meter for sale Message-ID: <6D5647BB396F499E81AA85B98392F244@shop1e2e996aa5> I have a Fisher Scientific Accumet hand held pH meter for sale. Cathy A. Mayton Wasatch Histo Consultants, Inc. From jcox90 <@t> yahoo.com Wed Apr 8 16:50:39 2009 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Wed Apr 8 16:50:42 2009 Subject: [Histonet] Part-Time Histologist position for private lab in Phoenix Message-ID: <363462.7991.qm@web56806.mail.re3.yahoo.com> Hi Netters, We have a part-time histologist position for a wonderful Dermatology lab here in Phoenix AZ. Hours are flexible, wonderful work environment, great Doctors, please call Jill at 602-481-1424 if interested or would like more info. Jill Cox HT (ASCP) ? ? ? ? From dermpathsy <@t> gmail.com Wed Apr 8 17:31:58 2009 From: dermpathsy <@t> gmail.com (Sate Hamza) Date: Wed Apr 8 17:32:02 2009 Subject: [Histonet] histonet off-topic comments In-Reply-To: <000d01c9b865$61d07970$25716c50$@net> References: <001501c9b856$555c4b60$0014e220$@net> <000d01c9b865$61d07970$25716c50$@net> Message-ID: <8854ff80904081531s52d10833ied1bef1310393bae@mail.gmail.com> Yes. I use Gmail, because my hospital gives only a mediocre 10 MBs of inbox space for the hospital account. Many others on the list use Yahoo or Gmail, or other provider email accounts. I use this Gmail account to subscribe to 5 or 6 mailing lists relevant to my practice. I have been a member of this list for a relatively short period of time, but it is quite evident to me that it is a very valuable resource. The few queries that I posted to the list all resulted in very helpful replies, without exception. I would not leave this list no matter what the problems are. I would trust the moderators to find a solution for any problem (e.g. turning the moderation bit on, for any disruptive list members, in Mailman, the list's software). If one is overwhelmed by the number of messages received daily, one can stop the delivery of messages temporarily by editing the personal options in the list's web page. Members should stop sending their "unsubscribe" request messages to the list. If one wants to unsubscribe, this can be done by sending a message to the list owner or by going to the list's page, and editing subscription options. On 4/8/09, Pamela Marcum wrote: > Unfortunately some of us don't use our work e-mail address due to rules or > convenience. Pam Marcum > > -----Original Message----- > From: Kevin Gillinder [mailto:k.r.gillinder@newcastle.ac.uk] > Sent: Wednesday, April 08, 2009 12:03 PM > To: Pamela Marcum; Andrea Grantham; LINDA MARGRAF > Cc: histonet@lists.utsouthwestern.edu; MKing > Subject: Re: [Histonet] histonet off-topic comments > > Easily Fixed. > > Remove any 'non-educational / institutional ' email addresses from the > listserv...... > > -kevin > > > On 08/04/2009 15:28, "Pamela Marcum" wrote: > > AMEN!! Andi - after a while you get tired of first reviewing every message > for the good ones and then deleting everything else as fast as you can > without opening them. This is too valuable a service to Histology to lose > over a few malicious individuals who obviously have no interest in the field > or what we do or worse may not even be histologists. > > Pam Marcum > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea > Grantham > Sent: Wednesday, April 08, 2009 10:19 AM > To: LINDA MARGRAF > Cc: histonet@lists.utsouthwestern.edu; MKing > Subject: Re: [Histonet] histonet off-topic comments > > Linda, > > This really is too much - now I have this thing on my computer > maliciously put there by "aa aa" that I can not get to close. More > than one person has to be removed from the list and if Bernie was > removed why are there emails from him this morning? Histonet has > helped me so much but I'm afraid this is the last straw. I'm going to > have to unsubscribe. Sad to see such a wonderful resource go down the > tubes because of a handful of inconsiderate bastards. > > Andi Grantham > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cell Biology and Anatomy > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > "happy slicing and dicing and may all your stains work perfectly" - > Paula Sicurello > > > > > On Apr 7, 2009, at 9:52 AM, LINDA MARGRAF wrote: > >> Dear Histonet members: >> I have removed Bernie Taupin from the list. >> Let's please get back to the topic of histology and related >> fields.Thanks >> Linda M >> Histonet administrator >> >>>>> MKing 4/7/2009 8:43 AM >>> >> a motion is hereby offered to have the active members of histonet vote >> this troll off the island, or at least request that the list moderator >> do so. this person has proven to have absolutely nothing of value to >> contribute to this group, and is only intent on disrupting it. >> >> second? >> >> ----------------- >> Message: 16 >> Date: Mon, 6 Apr 2009 15:43:07 -0700 (PDT) >> From: Bernie Taupin >> Subject: Re: [Histonet] Nice one Kemlo >> To: histonet@lists.utsouthwestern.edu >> Message-ID: <453473.65848.qm@web43516.mail.sp1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >>>> Instead of spitting your venom try to learn >> >> Sort of like you might consider learning correct English? I mean, its >> one thing if its not your first language, but despite how many >> people on >> here called you kindly and helpful, I find it perplexing that you feel >> compelled to call me a "piece of ignorant", and TWICE, no less. >> >> I do not speak French. This list is not in French. Ergo, my awareness >> (or lack thereof) of proper French diction has absolutely nothing at >> all >> to do with anything... aside form providing you a vehicle to call me >> names and lash out. >> >> If you have something to say, say it. The simple fact that you do not >> like me does not give you- or anyone else- wholesale right to insult >> me >> or call me names. >> >> You should be ashamed of yourself, Rene. I never expected such >> antisocial behavior from you, and frankly, when you behave so >> regrettably, I feel no compulsion to "learn" anything form you! >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> Please consider the >> environment before printing this e-mail
>>
>> >> This e-mail, facsimile, or letter and > >> any files or attachments transmitted with it contains
>> information that is confidential and privileged. This > information >> is intended only for the use of the
>> individual(s) and entity(ies) to whom it is addressed. If > you are >> the intended recipient, further
>> >> disclosures are prohibited without proper authorization. If > you >> are not the intended recipient, any
>> disclosure, copying, printing, or use of this information is > >> strictly prohibited and possibly a
>> violation of federal or state law and regulations. If you > have >> received this information in error,
>> please notify Children's Medical Center Dallas immediately > at >> 214-456-4444 or via e-mail at
>> privacy@childrens.com. Children's Medical Center Dallas and > its >> affiliates hereby claim all
>> applicable privileges related to this information.
/> >> >>
>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada From dermpathsy <@t> gmail.com Wed Apr 8 17:55:37 2009 From: dermpathsy <@t> gmail.com (Sate Hamza) Date: Wed Apr 8 17:55:56 2009 Subject: [Histonet] Colloidal Iron control In-Reply-To: <8854ff80904081504y4b72be4eif02840c29d6e6a98@mail.gmail.com> References: <49DC7BE5.4AA8.00C0.0@ah.org> <8854ff80904081504y4b72be4eif02840c29d6e6a98@mail.gmail.com> Message-ID: <8854ff80904081555y6ba03f5bn45b8a6277401033f@mail.gmail.com> A skin biopsy from a discoid lupus erythematosus (DLE) case. Sate On 4/8/09, Kathleen Boozer wrote: > What are you using? -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada From AnthonyH <@t> chw.edu.au Wed Apr 8 18:09:10 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Apr 8 18:09:17 2009 Subject: [Histonet] Colloidal Iron control In-Reply-To: <49DC7BE5.4AA8.00C0.0@ah.org> Message-ID: Kathleen, Controls: Mucin Control (Appendix/umbilical cord). Also include kidney and skin. A second slide should be left in water until step 6 (to detect endogenous iron) Alcian Blue (pH 2.5) can be run in parallel. Tickoo et al (1998 Am J Surg Pathol 22(4): 419-424) recommend that when staining a suggested chromophobe renal cell carcinoma, a block containing normal renal cortex, along with the tumour, be selected. Slides are acceptable for interpretation only if the glomerular mesangium stained distinctly and the tubular epithelium showed no staining. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Thursday, 9 April 2009 3:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Colloidal Iron control What are you using? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From cathy <@t> wasatchhisto.com Wed Apr 8 18:45:37 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Wed Apr 8 18:46:02 2009 Subject: [Histonet] wood slide boxes for sale Message-ID: <034B7988DDEC4EB999238AEA3E0A2D01@shop1e2e996aa5> I have 5 nice wood slide boxes that hold 100 slides for sale Cathy A. Mayton Wasatch Histo Consultants, Inc. From bernietaupin <@t> ymail.com Wed Apr 8 20:03:02 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 8 20:03:06 2009 Subject: [Histonet] UNSUBSCRIBE Message-ID: <355849.29836.qm@web43507.mail.sp1.yahoo.com> I've heard a few other people mention it, but you know that replying "UNSUBSCRIBE" to the entire list doesn't do anything but send an email containing only the word "UNSUBSCRIBE" out to all the list-members, yeah? ________________________________ From: Andrea Grantham To: HISTONET Sent: Wednesday, April 8, 2009 10:21:58 AM Subject: [Histonet] UNSUBSCRIBE UNSUBSCRIBE Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cell Biology and Anatomy Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 "happy slicing and dicing and may all your stains work perfectly" - Paula Sicurello _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernietaupin <@t> ymail.com Wed Apr 8 20:03:38 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 8 20:03:43 2009 Subject: [Histonet] "FREEZY" spray Message-ID: <234467.40041.qm@web43515.mail.sp1.yahoo.com> I see that Kemlo is an HistoNet charlatan like the rest of us. He's the first complain about off-topic notes to the list, then goes on to send several of his own. Thank you Uncle Kemlo! :-P Way to keep this ball rolling! ________________________________ From: Kemlo Rogerson To: Tony Henwood ; Bernie Taupin ; Akemi Allison-Tacha ; Jennifer MacDonald ; Ingles Claire Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 4:05:39 AM Subject: RE: [Histonet] "FREEZY" spray Say thank you Uncle Kemlo!! Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: 08 April 2009 08:58 To: Bernie Taupin; Akemi Allison-Tacha; Jennifer MacDonald; Ingles Claire Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] "FREEZY" spray Hey, Would you believe that putting the word "Taupin" into my outlook rules actually works. Now every obnoxious post from "Taupin" actually disappears into my Junk folder and I do not have to read the drivel. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, 8 April 2009 3:38 PM To: Akemi Allison-Tacha; Jennifer MacDonald; Ingles Claire Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] "FREEZY" spray Sorry, again, I'm confused... are you responding to me? I'm not the original poster... I never stated I had trouble with cracking, because, well, I don't. I'm Bernie Taupin, the King of Cryomicrotomy, Esq. ________________________________ From: Akemi Allison-Tacha To: Jennifer MacDonald ; Ingles Claire ; Bernie Taupin Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:27:35 AM Subject: Re: [Histonet] "FREEZY" spray Sorry, I too noticed that the text looked weird then I did a cut and paste. I keep all the Data Sheets I created in my files, and this was just a portion of the Notes from the original Data sheet. I thought it might be helpful because you stated you had difficulty with cracking. You did not mention using isopentane. Some people immerse the tissue straight into liquid nitrogen, which could cause freezing artifacts. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com --- On Tue, 4/7/09, Bernie Taupin wrote: From: Bernie Taupin Subject: Re: [Histonet] "FREEZY" spray To: "Akemi Allison-Tacha" , "Jennifer MacDonald" , "Ingles Claire" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 10:11 PM I use isopentane suspended in liquid nitrogen, too. sorry if this sounds curt, but due to your cut-and-pasting, im not entirely sure what point youre trying to get at...? ________________________________ From: Akemi Allison-Tacha To: Jennifer MacDonald ; Ingles Claire ; Bernie Taupin Cc: Histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 1:03:04 AM Subject: Re: [Histonet] "FREEZY" spray Bernie, I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. Years later, I developed the "MATSSE (pH3.4) 1-Step Trichrome Stain Kit" (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. This kit has long since discontinued by the way....This came from Biocare's Data Sheet. NOTE:Muscle tissue should be suitably obtained and frozen within 30 minutes after excision. The usual method of freezing is to first mount a transverse section of the muscle on a chuck using 10% tragacanth gum or equivalent commercial frozen section mounting media as the adhesive. The chuck with the mounted specimen is then immersed in prechilled isopentane until frozen. Isopentane is precooled when a container of the substance is placed into liquid nitrogen. At a temperature of -160? C, the isopentane has a slightly syrupy consistency. Care should be taken to remove the muscle sample when freezing is complete, usually after 25 to 30 seconds.. Too short a freezing time produces artifacts; prolonged freezing can produce cracking of the block. NOTE: If the sample cannot be sectioned immediately after freezing, it may be wrapped in foil and placed in a plastic-lidded container along with a small amount of ice for moisture. Specimens may be stored at -70? C until sectioned.. Regards, Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Court, Los Gatos, CA 95032 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo..com --- On Tue, 4/7/09, Bernie Taupin wrote: From: Bernie Taupin Subject: Re: [Histonet] "FREEZY" spray To: "Jennifer MacDonald" , "Ingles Claire" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Date: Tuesday, April 7, 2009, 9:06 PM > Does this apply to Liquid Nitrogen as well? I'd hate to have to try to > cut fatty sections in the cryostat without it! I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about? I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen. kisses, flowers and rainbows, Bernie Taupin, King of Cryomicrotomy, Esq. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jacobc <@t> mmc.org Wed Apr 8 20:10:01 2009 From: jacobc <@t> mmc.org (Christine Jacobs) Date: Wed Apr 8 20:10:12 2009 Subject: [Histonet] unsubscribe Message-ID: <49DD12A9.C070.0066.0@mmc.org> Please cancel my subscription to histonet CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the use of the intended recipient(s) only and may contain information that is privileged, confidential, and prohibited from unauthorized disclosure under applicable law. If you are not the intended recipient of this message, any dissemination, distribution, or copying of this message is strictly prohibited. If you received this message in error, please notify the sender by reply email and destroy all copies of the original message and attachments. From sccrshlly <@t> yahoo.com Wed Apr 8 20:17:22 2009 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Wed Apr 8 20:17:25 2009 Subject: [Histonet] Re: "burned tissue" Message-ID: <34705.84538.qm@web90305.mail.mud.yahoo.com> Ann, ? We had this problem at one time and found that the tissue was being left out to dry during grossing, and the result was that the sections were actually cutting thicker due to difficulty sectioning.? Is this a possibility for you?? The drying out could also happen at the physicians office as well, if they lay them out on gauze and then put them in formalin.? The biopsies dry out quickly due to their size. ? Good luck, Shelly From jqb7 <@t> cdc.gov Wed Apr 8 20:22:38 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Apr 8 20:28:22 2009 Subject: [Histonet] unsubscribe In-Reply-To: <49DD12A9.C070.0066.0@mmc.org> References: <49DD12A9.C070.0066.0@mmc.org> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF0551@LTA3VS011.ees.hhs.gov> In order to unsubscribe please follow the directions you were provided when you signed up initially. You must actually do it yourself. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christine Jacobs Sent: Wednesday, April 08, 2009 9:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] unsubscribe Please cancel my subscription to histonet CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the use of the intended recipient(s) only and may contain information that is privileged, confidential, and prohibited from unauthorized disclosure under applicable law. If you are not the intended recipient of this message, any dissemination, distribution, or copying of this message is strictly prohibited. If you received this message in error, please notify the sender by reply email and destroy all copies of the original message and attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dbpiontek <@t> hotmail.com Wed Apr 8 20:49:06 2009 From: dbpiontek <@t> hotmail.com (Denise Piontek) Date: Wed Apr 8 20:49:10 2009 Subject: [Histonet] Controls needed! In-Reply-To: <5F3F860CFE0F4741B1D87A88A58FAE9A256101@mailbe01.mc.vanderbilt.edu> References: <4F0B7161A6CD524FAD8017D52E1553400A768881@exchangent> <5F3F860CFE0F4741B1D87A88A58FAE9A256101@mailbe01.mc.vanderbilt.edu> Message-ID: Newcomer Supply has been a reliable control source, when necessary the have "customized orders". > Date: Wed, 8 Apr 2009 07:14:21 -0500 > From: jennifer.l.hofecker@Vanderbilt.Edu > To: DKnutson@primecare.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Controls needed! > CC: > > Hi Deanne, > Have you checked with the NSH Control Tissue Bank? The form to request > tissue is online on the NSH web page (www.nsh.org). The bank is run by > the Quality Control committee in conjunction with the IHCRG. It is a > free service to NSH members. > You may contact the committee chair, William DeSalvo at > wdesalvo.cac@hotmail.com with any specific questions. > Have a great week. > > Jennifer L. Hofecker HT(ASCP) > Vanderbilt University Medical Center > Division of Neuropathology > Nashville, TN > ph 615.343.0083 > fax 615.343.7089 > -----Original Message----- > From: Knutson, Deanne [mailto:DKnutson@primecare.org] > Sent: Tuesday, April 07, 2009 3:06 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Controls needed! > > We are looking for H. Pylori control tissue and also GMS/fungus control > tissue. Is there anyone out there that might have extra to share? We > have > good GRAM control blocks that we would be happy to exchange. Please let > me > know if you can help us out. Thank you! > > > > Deanne Knutson > > Anatomic Pathology Supervisor > > St. Alexius Medical Center > > Bismarck, N. Dak. 58506 > > 701-530-6730 > > Fax 701-530-6735 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Rediscover Hotmail?: Get quick friend updates right in your inbox. http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Updates1_042009 From bernietaupin <@t> ymail.com Wed Apr 8 23:05:16 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Wed Apr 8 23:05:21 2009 Subject: [Histonet] unsubscribe In-Reply-To: <9A16CB5D55FC1648ADF11B63E72A1BE1BF0551@LTA3VS011.ees.hhs.gov> References: <49DD12A9.C070.0066.0@mmc.org> <9A16CB5D55FC1648ADF11B63E72A1BE1BF0551@LTA3VS011.ees.hhs.gov> Message-ID: <367521.27352.qm@web43511.mail.sp1.yahoo.com> You'd think that managing one's subscription to a mailing-list was rocket-science or something! ________________________________ From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: Christine Jacobs ; histonet@lists.utsouthwestern.edu Sent: Wednesday, April 8, 2009 9:22:38 PM Subject: RE: [Histonet] unsubscribe In order to unsubscribe please follow the directions you were provided when you signed up initially. You must actually do it yourself. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christine Jacobs Sent: Wednesday, April 08, 2009 9:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] unsubscribe Please cancel my subscription to histonet CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the use of the intended recipient(s) only and may contain information that is privileged, confidential, and prohibited from unauthorized disclosure under applicable law. If you are not the intended recipient of this message, any dissemination, distribution, or copying of this message is strictly prohibited. If you received this message in error, please notify the sender by reply email and destroy all copies of the original message and attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From u_deserve_2_know <@t> yahoo.com Wed Apr 8 23:41:35 2009 From: u_deserve_2_know <@t> yahoo.com (u know) Date: Wed Apr 8 23:41:40 2009 Subject: [Histonet] Spyware from an Attachment: Message-ID: <251132.29274.qm@web38203.mail.mud.yahoo.com> Dear Listers, If you ever opened any strange attachments from this list, you should really consider scanning your Hard Disk for spyware. There is a great free anti-spyware program we use, which you can get at http://tinyurl.com/ynupj4 <----that is a virus-proof link, by the way. From tissuearray <@t> hotmail.com Thu Apr 9 00:27:18 2009 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Thu Apr 9 00:27:22 2009 Subject: [Histonet] TMA for cell pellets? In-Reply-To: <1089A756B010074CA09E65CD92401608018A8C8B@MEDMAIL.urmc-sh.rochester.edu> References: <1089A756B010074CA09E65CD92401608018A8C8B@MEDMAIL.urmc-sh.rochester.edu> Message-ID: Have you tried to embed your cell pellets into a tissue microarray? Two companies I would recommend: www.arraymold.com www.beecherinstruments.com Two of the best TMA products on the market. cheers, > Date: Thu, 2 Apr 2009 12:05:39 -0400 > From: Louise_Hartson@URMC.Rochester.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Embedding cell pellets > > > I am looking for a protocol for embedding cell pellets in paraffin. > Thanks, > Louise > > Louise Hartson, BA > Senior Technical Associate > University of Rochester > Louise_Hartson@URMC.Rochester.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009 From bernietaupin <@t> ymail.com Thu Apr 9 00:50:26 2009 From: bernietaupin <@t> ymail.com (Bernie Taupin) Date: Thu Apr 9 00:50:29 2009 Subject: [Histonet] TMA for cell pellets? In-Reply-To: References: <1089A756B010074CA09E65CD92401608018A8C8B@MEDMAIL.urmc-sh.rochester.edu> Message-ID: <338565.20208.qm@web43513.mail.sp1.yahoo.com> Recently, someone mentioned putting a library of protocols online, perhaps in the HistoNet archives. I'd like to second that emotion if its possible to make happen... What a great resource that would be! ________________________________ From: Thom Jensen To: louise_hartson@urmc.rochester.edu; histonet@lists.utsouthwestern.edu Sent: Thursday, April 9, 2009 1:27:18 AM Subject: [Histonet] TMA for cell pellets? Have you tried to embed your cell pellets into a tissue microarray? Two companies I would recommend: www.arraymold.com www.beecherinstruments.com Two of the best TMA products on the market. cheers, > Date: Thu, 2 Apr 2009 12:05:39 -0400 > From: Louise_Hartson@URMC.Rochester.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Embedding cell pellets > > > I am looking for a protocol for embedding cell pellets in paraffin. > Thanks, > Louise > > Louise Hartson, BA > Senior Technical Associate > University of Rochester > Louise_Hartson@URMC.Rochester.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonet.nospam <@t> vneubert.com Thu Apr 9 03:07:54 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Thu Apr 9 03:08:01 2009 Subject: [Histonet] To all spam gourmets Message-ID: <49DDACDA.8040503@vneubert.com> Please, stop spamming this list. Comment if you know something useful. Otherwise keep it. I don't mind what you had for breakfast in June 1st last year or your opinion on my clothes' colour. Believe it or not, there IS a real life ( link: http://en.wikipedia.org/wiki/Real_life_(reality) ), and maybe you should check it out - it has way more to offer than waiting for responses to mock about. Thanks. Valentin PS: What about a moderated board? Every inappropriate comment on HISTO TOPIC could be moved or even deleted, except in the spamming forum which can be found on nearly every board on the net. From hlukey <@t> msn.com Thu Apr 9 03:13:22 2009 From: hlukey <@t> msn.com (Hugh Luk) Date: Thu Apr 9 03:13:26 2009 Subject: [Histonet] I recommend NOT opening that Attachment: In-Reply-To: <251132.29274.qm@web38203.mail.mud.yahoo.com> References: <251132.29274.qm@web38203.mail.mud.yahoo.com> Message-ID: Fellow histology and related fields professionals, I'm not opening this. From a call name of "u know (u_deserve_2_know@yahoo.com)." The last time I opened something like this, from a name like this, I ended up owing money for Viagra treatments for Panda bears or something like that. It may be real, but it probably is spam. There...has been...a lot...of it...recently! By the way, there is no such thing as a "Virus-proof LINK!" Not sure? Just Google it. If you need Spyware or Antivirus software, check your IT department. Also, CNET has free downloads of AdAware, or AVG antivirus 8. And now for a joke (I think we all need it); A physician is doing his rounds and a nurse stops him in the hallway, "Doctor, can you sign these reports?" "Fine" he says and proceeds to pull out a rectal thermometer from his coat pocket. He looks at it in disbelief and exclaims, "Darn it! Some a$$ has my pen!" Respectfully, Hugh Luk, HTL (ASCP) Cancer Research center of Hawaii Pathology shared resources lab manager KP- Hawaii > Date: Wed, 8 Apr 2009 21:41:35 -0700 > From: u_deserve_2_know@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Spyware from an Attachment: > > Dear Listers, > > If you ever opened any strange attachments from this list, you should really consider scanning your Hard Disk for spyware. There is a great free anti-spyware program we use, which you can get at http://DELETED<----that is a virus-proof link, by the way. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Quick access to your favorite MSN content and Windows Live with Internet Explorer 8. http://ie8.msn.com/microsoft/internet-explorer-8/en-us/ie8.aspx?ocid=B037MSN55C0701A From Louise_Hartson <@t> URMC.Rochester.edu Thu Apr 9 07:29:23 2009 From: Louise_Hartson <@t> URMC.Rochester.edu (Hartson, Louise) Date: Thu Apr 9 07:32:09 2009 Subject: [Histonet] TMA for cell pellets? References: <1089A756B010074CA09E65CD92401608018A8C8B@MEDMAIL.urmc-sh.rochester.edu> <338565.20208.qm@web43513.mail.sp1.yahoo.com> Message-ID: <1089A756B010074CA09E65CD92401608018A8CAC@MEDMAIL.urmc-sh.rochester.edu> Thank you to everyone who responded!! I was given the cell pellets already in the matrix so I only had to process!! It was easier than the mouse tissues!! Since we do so few 20-40 at a time I do everything by hand...no automation in our lab!! -----Original Message----- From: Bernie Taupin [mailto:bernietaupin@ymail.com] Sent: Thu 4/9/2009 1:50 AM To: Thom Jensen; Hartson, Louise; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] TMA for cell pellets? Recently, someone mentioned putting a library of protocols online, perhaps in the HistoNet archives. I'd like to second that emotion if its possible to make happen... What a great resource that would be! ________________________________ From: Thom Jensen To: louise_hartson@urmc.rochester.edu; histonet@lists.utsouthwestern.edu Sent: Thursday, April 9, 2009 1:27:18 AM Subject: [Histonet] TMA for cell pellets? Have you tried to embed your cell pellets into a tissue microarray? Two companies I would recommend: www.arraymold.com www.beecherinstruments.com Two of the best TMA products on the market. cheers, > Date: Thu, 2 Apr 2009 12:05:39 -0400 > From: Louise_Hartson@URMC.Rochester.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Embedding cell pellets > > > I am looking for a protocol for embedding cell pellets in paraffin. > Thanks, > Louise > > Louise Hartson, BA > Senior Technical Associate > University of Rochester > Louise_Hartson@URMC.Rochester.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail? is up to 70% faster. Now good news travels really fast. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Apr 9 08:56:21 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 9 08:56:25 2009 Subject: [Histonet] Formula 83 users? In-Reply-To: Message-ID: <613905.80912.qm@web65715.mail.ac4.yahoo.com> Jacqueline: Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant. Ren? J. --- On Wed, 4/8/09, Jacqueline Farnsworth wrote: From: Jacqueline Farnsworth Subject: [Histonet] Formula 83 users? To: "Histonet" Date: Wednesday, April 8, 2009, 12:31 PM HI all. I have searched the Histonet archives and found a lot of very positive reviews regarding Formula 83. I am wondering if anyone has encountered any issues with using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there any miscibility issues? Are you soaking in xylene prior to tape coverslipping required? Any helpful hints/tips/tricks? Thank you in advance. Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Apr 9 08:58:45 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 9 08:58:50 2009 Subject: [Histonet] Negative IHC controls In-Reply-To: <858441.67063.qm@web57806.mail.re3.yahoo.com> Message-ID: <292901.13223.qm@web65712.mail.ac4.yahoo.com> I used to run a negative control per case or per block within any given case when doing IHC, but not for reagents. Ren? J. --- On Wed, 4/8/09, Sheila Haas wrote: From: Sheila Haas Subject: [Histonet] Negative IHC controls To: histonet@lists.utsouthwestern.edu Date: Wednesday, April 8, 2009, 2:57 PM I have a question concerning ANP.22570 on the CAP checklist.?Could?you all tell me?how you are handling negative controls for IHC staining? The question actually states (and I've confirmed with CAP) that we should be running two types of negative controls. One for reagents and one for?each antibody in a run. I'd like to know what?the practice is. This seems very costly and time consuming. Thanks in advance! ? Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Thu Apr 9 09:14:48 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Apr 9 09:09:53 2009 Subject: [Histonet] Formula 83 users? In-Reply-To: <613905.80912.qm@web65715.mail.ac4.yahoo.com> References: <613905.80912.qm@web65715.mail.ac4.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E3908778D156E@IBMB7Exchange.digestivespecialists.com> With all due respect Ren?; I have been using Formula 83 with my coverslipper with no problems at all though have a glass coverslipper not a tape. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, April 09, 2009 9:56 AM To: Histonet; Jacqueline Farnsworth Subject: Re: [Histonet] Formula 83 users? Jacqueline: Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant. Ren? J. --- On Wed, 4/8/09, Jacqueline Farnsworth wrote: From: Jacqueline Farnsworth Subject: [Histonet] Formula 83 users? To: "Histonet" Date: Wednesday, April 8, 2009, 12:31 PM HI all. I have searched the Histonet archives and found a lot of very positive reviews regarding Formula 83. I am wondering if anyone has encountered any issues with using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there any miscibility issues? Are you soaking in xylene prior to tape coverslipping required? Any helpful hints/tips/tricks? Thank you in advance. Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Thu Apr 9 09:11:11 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Apr 9 09:12:14 2009 Subject: [Histonet] Spyware from an Attachment: In-Reply-To: <251132.29274.qm@web38203.mail.mud.yahoo.com> References: <251132.29274.qm@web38203.mail.mud.yahoo.com> Message-ID: <49DE01FF.100@umdnj.edu> Dear List: I strongly suggest that you NOT use any links offered by people with no name and no affiliation. Yes, there are good spyware detection programs available at no cost (Adaware for example) but I don't think this is one of them. There are at least 3 potential saboteurs on this list. Geoff u know wrote: > Dear Listers, > > If you ever opened any strange attachments from this list, you should really consider scanning your Hard Disk for spyware. There is a great free anti-spyware program we use, which you can get at http://tinyurl.com/ynupj4 <----that is a virus-proof link, by the way. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Jacqueline.Farnsworth <@t> cls.ab.ca Thu Apr 9 09:12:54 2009 From: Jacqueline.Farnsworth <@t> cls.ab.ca (Jacqueline Farnsworth) Date: Thu Apr 9 09:12:58 2009 Subject: [Histonet] Formula 83-Thank you! Message-ID: Thank you all for taking time to respond to my inquiry about Formula 83. We received a free sample and will perform some trials and I will post our results on the Histonet asap. Of course, there can always be hidden challenges bringing reagents over the Canadian border, so that in itself can prove interesting, (eh!) Thanks Jacquie Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From fudo <@t> ufl.edu Thu Apr 9 09:25:56 2009 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Thu Apr 9 09:26:00 2009 Subject: [Histonet] HISTOS 5 processor Message-ID: <1520390329.62531239287156589.JavaMail.osg@osgjas01.cns.ufl.edu> Hi, all We get some money recently and want to spend it on the HISTOS 5 proceesor from Smilestone. But we are not sure whether it is worth purchasing. Does anyone here have any experiences or suggestions of it? Thank you, Ann Fu University of Flodrida From alyssa <@t> alliedsearchpartners.com Thu Apr 9 09:25:55 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Apr 9 09:26:03 2009 Subject: [Histonet] Position Open In Mass Message-ID: Position Open in Mass Good morning and Happy Spring to everyone. I have a full time permanent position open in Burlington, Ma area. I have included the details below, and don?t forget about our $1000 referral program in place. Please forward this to whomever you feel fit, and if we place one of your referrals in a position than we pay you $1000 as a referral bonus. Thank you histonet and have a great day! *Qualifications: * - Necropsies experience needed - B.S. in Life Sciences or equivalent experience - Animal handling & restraint experience with both large and small animals - Dose administration and biological sample collection techniques experience needed - Ability to lift 50 lbs. *Title: * Pharma Technician *Department:* * * Pharma/chem. Services *Status: *Exempt *Shift: *Monday-Friday, Full Time, 8am-5pm, and as required for study performance and weekends. Weekly schedule may be Tuesday-Sat. or Sunday-Thursday. *Benefits: *Healthcare benefits, PTO, Vacation time, 401K, and much more! -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 From robert_schoonhoven <@t> yahoo.com Thu Apr 9 10:36:09 2009 From: robert_schoonhoven <@t> yahoo.com (Robert Schoonhoven) Date: Thu Apr 9 10:36:12 2009 Subject: [Histonet] HISTOS 5 processor In-Reply-To: <1520390329.62531239287156589.JavaMail.osg@osgjas01.cns.ufl.edu> References: <1520390329.62531239287156589.JavaMail.osg@osgjas01.cns.ufl.edu> Message-ID: <519647.24613.qm@web31104.mail.mud.yahoo.com> Ann, I was able to investigate this unit at Milestone Medical as their US headquarters is located locally here in Kalamazoo, MI. They have an excellent line of products and I recommend them highly. Disclaimer: I have no commercial interest in Milestone Medical. Regards, Bob Schoonhoven, HT/HTL (ASCP) ________________________________ From: "FU,DONGTAO" To: Histonet Sent: Thursday, April 9, 2009 10:25:56 AM Subject: [Histonet] HISTOS 5 processor Hi, all We get some money recently and want to spend it on the HISTOS 5 proceesor from Smilestone. But we are not sure whether it is worth purchasing. Does anyone here have any experiences or suggestions of it? Thank you, Ann Fu University of Flodrida _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robert_schoonhoven <@t> yahoo.com Thu Apr 9 10:44:51 2009 From: robert_schoonhoven <@t> yahoo.com (Robert Schoonhoven) Date: Thu Apr 9 10:44:56 2009 Subject: [Histonet] Travelling Histotechs Message-ID: <788204.30784.qm@web31104.mail.mud.yahoo.com> I have noticed in the past several posts looking for traveling histo techs. Would one of these agencies please contact me off list? 35+ years of experience recently become available. Robert Schoonhoven HT/HTL (ASCP) 269-615-0576 From Kris.Caldwell <@t> leica-microsystems.com Thu Apr 9 11:31:11 2009 From: Kris.Caldwell <@t> leica-microsystems.com (Kris.Caldwell@leica-microsystems.com) Date: Thu Apr 9 11:31:22 2009 Subject: [Histonet] Regional Sales Manager position- West Coast Message-ID: This person will need to be based on the West Coast, California preferred. Leica Microsystems is a leading global designer and producer of innovative high-tech precision optics systems for the analysis of microstructures. It is one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries This person will drive annual sales strategies and goals through management of Sales Representatives and field technical support staff. Develop area sales plans in conjunction with the Biosystems Division Director of Sales to support Leica?s Histology customers.? Provide the direction, leadership, coaching and mentoring for direct sales representatives.? Advance the professional development, professional conduct, sales effectiveness and efficiency of staff. Regional Sales Management Achieve and exceed Area?s quarterly and annual sales quota, strategic and unit sales mix objectives, profit goal and market share goals. Develop sales plans and strategies to increase market penetration within Area. Participate in annual sales strategy and OPEX planning. Develop annual sales quotas for sales representatives and dealers. Manage and control department expenses at or below budgeted levels. Approve pricing , within authority, for new and demonstration instrument sales. Maintain strategy, support and pricing consistency between sales territories for National/Regional Accounts and National contracts. Maintain relationships with key customers and influencers at major institutions in Area. Sales Representative Management Manage, coach, mentor and support a team of direct sales representatives. Ensure direct sales representative, specialists and ERD?s achieve and exceed individual quarterly and annual sales quota, strategic and unit sales mix objectives. Travel with sales representatives and specialists providing individual coaching and professional development to members of the regional selling team (target 3 days per week or 60% of available time). Complete Field Sales Management Reports and distribute report for each field visit. Establish Standards of Performance for direct reports and set expectations on completion of responsibilities and duties. Ensure direct reports are compliant with all aspects of Leica CRM tool. Ensure sales team adherence to and consistent use of the Leica Value Selling tool. Experience Required BA/BS Degree in Life Science or equivalent 5 plus years experience in sales Preferred MBA/Masters Degree in Life Science 3 plus years sales management experience in selling capital equipment Histology/Cytology/Immunohistochemistry market knowledge Experience in distributor management a plus Leica offers competitive salary, benefits including medical, dental, vision, prescription, long-term care, life insurance, STD, LTD, and 401 (k). Please email your resume to- kris.caldwell@leica-microsystems.com Kris Caldwell Human Resources Recruiter Leica Microsystems, Inc. 2345 Waukegan Road Bannockburn, IL 60015 www.leica-microsystems.com Kris.Caldwell@Leica-Microsystems.com 847-405-5432 - phone 847-236-3035 - fax 847-323-6169- cellular ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From putnamj <@t> ggclinic.com Thu Apr 9 11:50:06 2009 From: putnamj <@t> ggclinic.com (Putnam, Jodi) Date: Thu Apr 9 11:50:20 2009 Subject: [Histonet] New lab, need some info Message-ID: Hi, I called the NSH and they said I might have better luck posting on the histonet but they are sending me all that they have in the way of help in writing manuals for the lab. I am working in a new lab that just opened a few months ago, all derm, building from the ground up. I have all my log sheets generated (all that I can think of anyway) to document temps, maintenance on machines, non pathologist grossing log, block and slide count etc. I have to write all my manuals from scratch. I know that they have to be in NCCLS format but was wondering if anyone could just give me some advise. I have my first inspection, by the state, in August and we are eventually going to be CAP certified. So, I don't want to miss anything. I am the only tech and doing all the grossing, typing my transcription, ordering, basic histology, special stains, IF etc. Any words of wisdom would be greatly appreciated. I just don't want to miss anything and I have OCD and worry myself into a frenzy. I work for a great doctor and want her practice to grow rapidly and I know it will. Thanks for any help you can give. Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From jtaylor <@t> meriter.com Thu Apr 9 13:11:41 2009 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Thu Apr 9 13:11:44 2009 Subject: [Histonet] TdT Message-ID: <328CBAE62F31C642B422970E879DFADC03A36A0A@pcwex01> I'm wondering where labs are purchasing their TdT antibody from. I'm currently using the Dako Autostainer and would be interested in any information on the procedure as well. Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI From JMacDonald <@t> mtsac.edu Thu Apr 9 13:26:52 2009 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Apr 9 13:28:52 2009 Subject: [Histonet] New lab, need some info In-Reply-To: Message-ID: NCCLS is now known as CLSI. They have a standard (document) for the writing of procedure manuals. CAP will require this, so you may want to purchase that document now. "Putnam, Jodi" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/09/2009 09:51 AM To cc Subject [Histonet] New lab, need some info Hi, I called the NSH and they said I might have better luck posting on the histonet but they are sending me all that they have in the way of help in writing manuals for the lab. I am working in a new lab that just opened a few months ago, all derm, building from the ground up. I have all my log sheets generated (all that I can think of anyway) to document temps, maintenance on machines, non pathologist grossing log, block and slide count etc. I have to write all my manuals from scratch. I know that they have to be in NCCLS format but was wondering if anyone could just give me some advise. I have my first inspection, by the state, in August and we are eventually going to be CAP certified. So, I don't want to miss anything. I am the only tech and doing all the grossing, typing my transcription, ordering, basic histology, special stains, IF etc. Any words of wisdom would be greatly appreciated. I just don't want to miss anything and I have OCD and worry myself into a frenzy. I work for a great doctor and want her practice to grow rapidly and I know it will. Thanks for any help you can give. Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Samuel_Perry <@t> DFCI.HARVARD.EDU Thu Apr 9 13:31:15 2009 From: Samuel_Perry <@t> DFCI.HARVARD.EDU (Perry, Samuel) Date: Thu Apr 9 13:31:19 2009 Subject: [Histonet] Edge effect on IHC-P Message-ID: Hi All, I am having variable staining when performing IHC. I seem to get an edge effect where the edge of the tissue shows increased DAB staining compared to the middle of the tissue. I have tried testing many fixation variables and still haven't found an edge affect culprit. I'm wondering if anyone in the community has experienced and corrected this problem. Thanks for you help! -Sam Perry Dana Farber Cancer Institute Boston MA Research Technician The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From shive003 <@t> umn.edu Thu Apr 9 13:45:17 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Apr 9 13:45:22 2009 Subject: [Histonet] Edge effect on IHC-P References: Message-ID: <327E0DFA077A490F977F4AF6F464B642@auxs.umn.edu> Sam, Could you give us a few facts from your protocol? They may help pin down what might be causing your edge effect. How long does your tissue stay on the grossing/trimming table before being put into fixative? What type of fixative are you using? How fresh is it? How long to you fix? What temps to you perform IHC at? How long are your incubations? Etc. Jan Shivers UMN VDL ----- Original Message ----- From: "Perry, Samuel" To: Sent: Thursday, April 09, 2009 1:31 PM Subject: [Histonet] Edge effect on IHC-P Hi All, I am having variable staining when performing IHC. I seem to get an edge effect where the edge of the tissue shows increased DAB staining compared to the middle of the tissue. I have tried testing many fixation variables and still haven't found an edge affect culprit. I'm wondering if anyone in the community has experienced and corrected this problem. Thanks for you help! -Sam Perry Dana Farber Cancer Institute Boston MA Research Technician The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Samuel_Perry <@t> DFCI.HARVARD.EDU Thu Apr 9 13:54:13 2009 From: Samuel_Perry <@t> DFCI.HARVARD.EDU (Perry, Samuel) Date: Thu Apr 9 13:54:18 2009 Subject: [Histonet] Edge effect IHC-P follow-up Message-ID: I am seeing this when I work with mouse tissue. After necropsy I extract the tissue from the mouse, cassette it, put it in fresh 10% NBF and let the tissue fix 24hrs before processing. I also see an edge effect when I fix with 4% PFA. During IHC-P I usually retrieve with a pressure cooker (120C) and citrate buffer solution. I use the DAKO envision kit and incubate the primary antibody at room temp. for 1 hour. I also see an edge affect when I take frozen tissue and thaw it in RT 10%NBF overnight followed by processing. The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From rjbuesa <@t> yahoo.com Thu Apr 9 14:12:38 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 9 14:12:41 2009 Subject: [Histonet] TdT In-Reply-To: <328CBAE62F31C642B422970E879DFADC03A36A0A@pcwex01> Message-ID: <576958.41984.qm@web65703.mail.ac4.yahoo.com> I used the Tdt from DAKO using their autostainer, diluted 1:10, HIER at pH8 and thymus as control. Ren? J. --- On Thu, 4/9/09, Taylor, Jean wrote: From: Taylor, Jean Subject: [Histonet] TdT To: histonet@lists.utsouthwestern.edu Date: Thursday, April 9, 2009, 2:11 PM I'm wondering where labs are purchasing their TdT antibody from. I'm currently using the Dako Autostainer and would be interested in any information on the procedure as well. Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ayreskyle <@t> yahoo.com Thu Apr 9 14:31:51 2009 From: ayreskyle <@t> yahoo.com (kyle ayres) Date: Thu Apr 9 14:31:54 2009 Subject: [Histonet] Effects of decalcification for special stains Message-ID: <417081.89096.qm@web31901.mail.mud.yahoo.com> Are there negative effects associated with acid decalcification and special stains, for example,?AFB, GMS, and PAS (for fungus). All tissues are FFPE. ? Thanks, Kyle?Ayres HT BS Nacogdoches Memorial Hospital? From SDrew <@t> uwhealth.org Thu Apr 9 15:35:29 2009 From: SDrew <@t> uwhealth.org (Drew Sally A) Date: Thu Apr 9 15:35:34 2009 Subject: [Histonet] INI1 antibody? Message-ID: <738A7878143FF74BB77436E255743C1A1F7B76@UWHC-MAIL03.uwhis.hosp.wisc.edu> Is anyone aware of a reference lab that has INI1 antibody one their menu? An inquiring neuropathologist wants to know... All positive responses will be happily forwarded to him... Thank you! Sally Ann Drew, MT (ASCP) sdrew@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 From rjbuesa <@t> yahoo.com Thu Apr 9 15:53:18 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 9 15:53:23 2009 Subject: [Histonet] Effects of decalcification for special stains In-Reply-To: <417081.89096.qm@web31901.mail.mud.yahoo.com> Message-ID: <883420.2362.qm@web65709.mail.ac4.yahoo.com> Usually not, unless the tissues remain too much time in the decalcifyier, meaning more time than needed to decalcify. For IHC the adverse?effect is greater and because of that, when possible, the decalficication should be done with EDTA. Ren? J. --- On Thu, 4/9/09, kyle ayres wrote: From: kyle ayres Subject: [Histonet] Effects of decalcification for special stains To: histonet@lists.utsouthwestern.edu Date: Thursday, April 9, 2009, 3:31 PM Are there negative effects associated with acid decalcification and special stains, for example,?AFB, GMS, and PAS (for fungus). All tissues are FFPE. ? Thanks, Kyle?Ayres HT BS Nacogdoches Memorial Hospital? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Thu Apr 9 17:08:25 2009 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Thu Apr 9 17:08:29 2009 Subject: [Histonet]( FE on BM Cores)Effects of decalcification for special stains Message-ID: <750263.58883.qm@web31305.mail.mud.yahoo.com> Don't forget the effects on?decalcified?Bone Marrow Cores. ?Even if you use EDTA decalcification solution, there will be diminished FE staining on Bone Marrow Cores or any other tissue that is decalcified and then stained for FE. Regards,Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 Cell: 425.941.4287? E-Mail: akemiat3377@yahoo.com --- On Thu, 4/9/09, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] Effects of decalcification for special stains To: histonet@lists.utsouthwestern.edu, "kyle ayres" Date: Thursday, April 9, 2009, 1:53 PM Usually not, unless the tissues remain too much time in the decalcifyier, meaning more time than needed to decalcify. For IHC the adverse?effect is greater and because of that, when possible, the decalficication should be done with EDTA. Ren? J. --- On Thu, 4/9/09, kyle ayres wrote: From: kyle ayres Subject: [Histonet] Effects of decalcification for special stains To: histonet@lists.utsouthwestern.edu Date: Thursday, April 9, 2009, 3:31 PM Are there negative effects associated with acid decalcification and special stains, for example,?AFB, GMS, and PAS (for fungus). All tissues are FFPE. ? Thanks, Kyle?Ayres HT BS Nacogdoches Memorial Hospital? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu Apr 9 17:28:18 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Apr 9 17:28:23 2009 Subject: [Histonet] Used Microtome Parts Available Message-ID: <57BE698966D5C54EAE8612E8941D768305554135@EXCHANGE3.huntingtonhospital.com> Are there any used equipment companies out there that would be interested in two used "Upper part for disposable blade carrier "E" ' for a Microm HM325 microtome? Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 From akemiat3377 <@t> yahoo.com Thu Apr 9 18:42:11 2009 From: akemiat3377 <@t> yahoo.com (akemiat3377@yahoo.com) Date: Thu Apr 9 18:42:13 2009 Subject: [Histonet] Not Histology:Early Friday and Safe Link RE: 10 most amazing images from Hubble Message-ID: <237955.21460.qm@web31305.mail.mud.yahoo.com> Hi All, I was deleting my unwanted e-mails last night and came across this link which is amazing and safe. ?If you haven't previewed this, you may want too. ?There is a higher power watching over us! ?http://www.dailymail.co.uk/legacygallery/gallery-9139/Hubble--The-amazing-space-photographs-universe.html?selectedImage=79814 Have a stellar day!Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032 Cell: 425.941.4287? E-Mail: akemiat3377@yahoo.com From akbitting <@t> geisinger.edu Thu Apr 9 20:13:43 2009 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Apr 9 20:14:05 2009 Subject: [Histonet] Benchmark XT In-Reply-To: <6C14869D8BA84515848C2072D84F65A5@JoePC> References: <20e8a532dd829543bf5bc49072095389@mail2.lcpath.com> <6C14869D8BA84515848C2072D84F65A5@JoePC> Message-ID: <49DE6507.2B7F.00C9.0@geisinger.edu> I just had the same problem with a bad lot of charged slides. It is, indeed, maddening. >>> "Joe Nocito" 4/8/2009 5:08 PM >>> another issue could be the mixer blowing too hard. I've had that happen too. JTT ----- Original Message ----- From: "Kari Bradshaw" To: "Dana Spears" ; ; "Phyllis Thaxton" Sent: Wednesday, April 08, 2009 2:13 PM Subject: RE: [Histonet] Benchmark XT We just recently went through a similar problem with irregular staining, light staining, sometimes no stain of any kind including counterstain,tissue falling off, and a handfull of false negative patient tissues, but positive controls (on the same slide). With two XT's running continuously it taken several days and everyone's help isolating the problem....not to mention several different lots numbers of slides....yikes! Next time I'll blame the slides right off the bat. Kari L. Bradshaw HT(ASCP) Laboratory Manager Lower Columbia Pathologists (360)425-5620 kbradshaw@lcpath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Spears Sent: Wednesday, April 08, 2009 9:24 AM To: histonet@lists.utsouthwestern.edu; Phyllis Thaxton Subject: Re: [Histonet] Benchmark XT I would definitely check your slides - we have found that when we get a bad lot of charged slides, things start washing off on the XT - regardless of detection, antibodies, whatever. It was happening to us on some tissue types and not on others, but a new batch of charged slides did the trick. I've never had to dry 60 min in the oven - 20 does it for us when we aren't having slide issues. Also, if you are using any Sta-on or any adhesive in your waterbath with the charged slides it can cause the slides to sort of repel the tissue and negate the "charged" effect.... again certain tissues will fall off, others stay on.... Dana Spears, HTL(ASCP) Laboratory Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) (309) 279-3768 (fax) dspears@mmci.org >>> Phyllis Thaxton 4/8/2009 8:43:36 AM >>> Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From jnocito <@t> satx.rr.com Thu Apr 9 20:30:23 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Apr 9 20:30:28 2009 Subject: [Histonet] Controls needed! In-Reply-To: References: <4F0B7161A6CD524FAD8017D52E1553400A768881@exchangent><5F3F860CFE0F4741B1D87A88A58FAE9A256101@mailbe01.mc.vanderbilt.edu> Message-ID: <857D8C00831545D89856F8E1A0B6A6A4@JoePC> I second that. Marsha is the loveliest person I know. ----- Original Message ----- From: "Denise Piontek" To: Sent: Wednesday, April 08, 2009 8:49 PM Subject: RE: [Histonet] Controls needed! Newcomer Supply has been a reliable control source, when necessary the have "customized orders". > Date: Wed, 8 Apr 2009 07:14:21 -0500 > From: jennifer.l.hofecker@Vanderbilt.Edu > To: DKnutson@primecare.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Controls needed! > CC: > > Hi Deanne, > Have you checked with the NSH Control Tissue Bank? The form to request > tissue is online on the NSH web page (www.nsh.org). The bank is run by > the Quality Control committee in conjunction with the IHCRG. It is a > free service to NSH members. > You may contact the committee chair, William DeSalvo at > wdesalvo.cac@hotmail.com with any specific questions. > Have a great week. > > Jennifer L. Hofecker HT(ASCP) > Vanderbilt University Medical Center > Division of Neuropathology > Nashville, TN > ph 615.343.0083 > fax 615.343.7089 > -----Original Message----- > From: Knutson, Deanne [mailto:DKnutson@primecare.org] > Sent: Tuesday, April 07, 2009 3:06 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Controls needed! > > We are looking for H. Pylori control tissue and also GMS/fungus control > tissue. Is there anyone out there that might have extra to share? We > have > good GRAM control blocks that we would be happy to exchange. Please let > me > know if you can help us out. Thank you! > > > > Deanne Knutson > > Anatomic Pathology Supervisor > > St. Alexius Medical Center > > Bismarck, N. Dak. 58506 > > 701-530-6730 > > Fax 701-530-6735 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Rediscover Hotmail?: Get quick friend updates right in your inbox. http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Updates1_042009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dchihc <@t> yahoo.com Thu Apr 9 20:50:02 2009 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Thu Apr 9 20:54:29 2009 Subject: [Histonet] Many Thanks Message-ID: <648479.12691.qm@web43502.mail.sp1.yahoo.com> I think our problem may very well be the slides. We tried many slides in the past and always came back to the superfrost brand that was most reliable, but I have found different opinions on these slides ;ately. I think it is time to change brands. Thank you all...Hope to see everyone in Birmingham Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From sheila_adey <@t> hotmail.com Fri Apr 10 05:30:28 2009 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Fri Apr 10 05:30:38 2009 Subject: [Histonet] Need a call from the Ventanna Rep please Message-ID: Hello, We are looking to possibly demo the Ventanna special stainer. Thanks. Sheila Adey HT MLT Port Huron Hospital Michigan _________________________________________________________________ Create a cool, new character for your Windows Live? Messenger. http://go.microsoft.com/?linkid=9656621 From relia1 <@t> earthlink.net Fri Apr 10 09:18:27 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Apr 10 09:18:33 2009 Subject: [Histonet] RELIA JOB Alert 4/10/09 Lead Histotechnologist opportunity in Springfield, MA Message-ID: Hi Histonetters! I hope you are having a great day. I have a new opportunity I want to tell you about! I am working with a premier client in Springfield, MA. My client is in need of a lead histotechnologist for a busy community surgical pathology lab. Knowledge of routine histology and immunohistochemistry is required. Knowledge of fluorescence and chromogenic in-situ hybridization techniques is a plus. In addition the ability to direct and support the work of other histotechnologists is required. This is a permanent fulltime M-F day shift position. My client offers a laboratory equipped with state of the art instrumentation, a competitive salary and excellent benefits. If you or someone you know might be interested please contact me. I can be reached toll free at 866-607-3542 or relia1@earthlink.net Happy Easter, Happy Passover and Have a Great Weekend! Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia From kenneth.metzger <@t> aruplab.com Fri Apr 10 09:21:57 2009 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Fri Apr 10 09:22:02 2009 Subject: [Histonet] Whole Mount Immuno Question (again) Message-ID: Can anyone tell me where to buy a slide holder or chamber to perform manual whole mount immunos? Something like the slide master from Scytek would be great but I won't get my hopes up!! Thanks, ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metzger@aruplab.com (801) 583-2787 x 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From laurie.colbert <@t> huntingtonhospital.com Fri Apr 10 09:11:54 2009 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Apr 10 10:18:27 2009 Subject: [Histonet] Benchmark XT Message-ID: <57BE698966D5C54EAE8612E8941D76830555418F@EXCHANGE3.huntingtonhospital.com> What kind of slides do you use?? Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, April 09, 2009 6:14 PM To: Kari Bradshaw; histonet@lists.utsouthwestern.edu; Dana Spears; Joe Nocito; Phyllis Thaxton Subject: Re: [Histonet] Benchmark XT I just had the same problem with a bad lot of charged slides. It is, indeed, maddening. >>> "Joe Nocito" 4/8/2009 5:08 PM >>> another issue could be the mixer blowing too hard. I've had that happen too. JTT ----- Original Message ----- From: "Kari Bradshaw" To: "Dana Spears" ; ; "Phyllis Thaxton" Sent: Wednesday, April 08, 2009 2:13 PM Subject: RE: [Histonet] Benchmark XT We just recently went through a similar problem with irregular staining, light staining, sometimes no stain of any kind including counterstain,tissue falling off, and a handfull of false negative patient tissues, but positive controls (on the same slide). With two XT's running continuously it taken several days and everyone's help isolating the problem....not to mention several different lots numbers of slides....yikes! Next time I'll blame the slides right off the bat. Kari L. Bradshaw HT(ASCP) Laboratory Manager Lower Columbia Pathologists (360)425-5620 kbradshaw@lcpath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Spears Sent: Wednesday, April 08, 2009 9:24 AM To: histonet@lists.utsouthwestern.edu; Phyllis Thaxton Subject: Re: [Histonet] Benchmark XT I would definitely check your slides - we have found that when we get a bad lot of charged slides, things start washing off on the XT - regardless of detection, antibodies, whatever. It was happening to us on some tissue types and not on others, but a new batch of charged slides did the trick. I've never had to dry 60 min in the oven - 20 does it for us when we aren't having slide issues. Also, if you are using any Sta-on or any adhesive in your waterbath with the charged slides it can cause the slides to sort of repel the tissue and negate the "charged" effect.... again certain tissues will fall off, others stay on.... Dana Spears, HTL(ASCP) Laboratory Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) (309) 279-3768 (fax) dspears@mmci.org >>> Phyllis Thaxton 4/8/2009 8:43:36 AM >>> Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cathy <@t> wasatchhisto.com Fri Apr 10 11:15:00 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Fri Apr 10 11:15:29 2009 Subject: [Histonet] glassware for sale Message-ID: <0E28829006B74240B937902317411A28@shop1e2e996aa5> I have the following glassware that needs a home Glass beakers 2 1000 ml 5 600 ml 1 400 ml 3 100 ml Plastic cylinders 1 500 ml 1 250 ml 2 100 ml Small glass staining dishes 4 dishes 3 staining racks Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 From tkngflght <@t> yahoo.com Fri Apr 10 12:42:24 2009 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Apr 10 12:42:27 2009 Subject: [Histonet] A Friday afternoon Funny for the H-net folks :) Message-ID: <618326.58291.qm@web50904.mail.re2.yahoo.com> This was forwarded to me by a path lab?friend--the link is clean and a nice giggle going into a holiday weekend. ? Cheryl Kerry, HT(ASCP) From: Dr. P.H. Sent: Friday, April 10, 2009 10:20 AM To: Subject: Critical Health Alert! ? ? As a physician, I am obligated to inform those individuals I care for about a critical health alert just released from the CDC in Atlanta , GA. ? The alert comes as the result of a huge study which was performed to try and explain a recent spike in unexplained illnesses. The researchers discovered that the inside of almost all computer screens are covered with bacteria, mites (and their feces), plus considerable microscopic industrial waste from the production of monitors overseas. All of this material seems to be the cause of the unexplained illnesses. Therefore, I urge you to immediately go to the link below to access a program specifically developed by the CDC to clean up and sterilize the inside of your monitor screen. Please, do this now so that I may rest at ease. Thank you. Phil Click on this link to clean the inside of your screen: http://www.raincitystory.com/flash/screenclean.swf? ? ? From Lynne.Bell <@t> cvmc.org Fri Apr 10 13:49:26 2009 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Fri Apr 10 13:49:31 2009 Subject: [Histonet] A Friday afternoon Funny for the H-net folks :) In-Reply-To: <618326.58291.qm@web50904.mail.re2.yahoo.com> Message-ID: Thanks, Cheryl! That's just what we needed after the week we have had here on the Histonet. Lynne Bell, HT(ASCP) Central Vermont Hospital 130 Fisher Road Barre, VT 05641 (802)371-4923 From schaundrawalton <@t> yahoo.com Fri Apr 10 14:16:41 2009 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Fri Apr 10 14:16:43 2009 Subject: [Histonet] Job Opportunity Message-ID: <83329.4748.qm@web58902.mail.re1.yahoo.com> I have a part-time HT/HTL day position open.??For more information please see the following link: ?http://www.swedishamerican.org/careers/job_opportunities/?a1=details&p=89 ? Please send all inquiries to the Human Resources department at (815) 966-2686, toll-free at (800) 322-4724, or by sending an e-mail message to: HR@swedishamerican.org.? ? -Schaundra Walton HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL From denise.woodward <@t> uconn.edu Fri Apr 10 15:11:11 2009 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Fri Apr 10 15:11:42 2009 Subject: [Histonet] Whole human feet In-Reply-To: <633861.20535.qm@web43505.mail.sp1.yahoo.com> References: <33b7cf2b0904061704g541684bdtd21d4dcd76469661@mail.gmail.com> <633861.20535.qm@web43505.mail.sp1.yahoo.com> Message-ID: I believe there is a computerized image file of an entire male and a female human body somewhere on the WWW. Maybe NIH?? These were whole mount histologic sections. Don't know if the plane is correct for your needs with regard to the sections of feet. News about it came out about 4-5 years ago. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 08, 2009 12:07 AM To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Whole human feet No offense, dude, but GROSS. That's probably why nobody has bitten yet, in regards to this query. ________________________________ From: Yak-Nam Wang To: histonet@lists.utsouthwestern.edu Sent: Monday, April 6, 2009 8:04:54 PM Subject: [Histonet] Whole human feet Hello all, If possible, we would like to obtain histological sections of adult human feet (plane of the anterior-posterior surface). Does anyone know of labs/groups that have done this or something similar? Thank you for your help Yak-Nam Wang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ynwang <@t> u.washington.edu Fri Apr 10 15:26:19 2009 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Fri Apr 10 15:26:23 2009 Subject: [Histonet] Whole human feet In-Reply-To: References: <33b7cf2b0904061704g541684bdtd21d4dcd76469661@mail.gmail.com> <633861.20535.qm@web43505.mail.sp1.yahoo.com> Message-ID: <33b7cf2b0904101326l352ce13dm7c1185e2c0329a7d@mail.gmail.com> Denise, Thanks, someone else pointed out these images and they are indeed in the correct plane (http://www.nlm.nih.gov/research/visible/image/feet.jpg). I think these are 1 mm sections from a frozen body, unstained. We were actually hoping that we could process feet (procured from a company), section and stain the sections with the basic H&E, modified Hart's, Picro sirius red and such like. Any help would be greatly appreciated. Thanks Yak-Nam On Fri, Apr 10, 2009 at 1:11 PM, Woodward, Denise wrote: > I believe there is a computerized image file of an entire male and a > female human body somewhere on the WWW. Maybe NIH?? These were whole mount > histologic sections. Don't know if the plane is correct for your needs with > regard to the sections of feet. News about it came out about 4-5 years ago. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin > Sent: Wednesday, April 08, 2009 12:07 AM > To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Whole human feet > > No offense, dude, but GROSS. > > That's probably why nobody has bitten yet, in regards to this query. > > > > > ________________________________ > From: Yak-Nam Wang > To: histonet@lists.utsouthwestern.edu > Sent: Monday, April 6, 2009 8:04:54 PM > Subject: [Histonet] Whole human feet > > Hello all, > > If possible, we would like to obtain histological sections of adult human > feet (plane of the anterior-posterior surface). Does anyone know of > labs/groups that have done this or something similar? > > Thank you for your help > Yak-Nam Wang > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Fri Apr 10 15:34:19 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 10 15:34:29 2009 Subject: [Histonet] Whole human feet In-Reply-To: <33b7cf2b0904101326l352ce13dm7c1185e2c0329a7d@mail.gmail.com> Message-ID: <654899.71755.qm@web65711.mail.ac4.yahoo.com> As far as I know such a large thin section that also involves decalcifying hard bones has never been published. In the past E. Leitz (now Leica) manufactured sliding microtomes for large specimens but were used mostly to prepare thin brain sections embedded in celloidin?but not for what you would like to do. If you are able to do it, for sure we all would like to know. Ren? J. --- On Fri, 4/10/09, Yak-Nam Wang wrote: From: Yak-Nam Wang Subject: Re: [Histonet] Whole human feet To: "Woodward, Denise" Cc: "histonet@lists.utsouthwestern.edu" Date: Friday, April 10, 2009, 4:26 PM Denise, Thanks, someone else pointed out these images and they are indeed in the correct plane (http://www.nlm.nih.gov/research/visible/image/feet.jpg). I think these are 1 mm sections from a frozen body, unstained. We were actually hoping that we could process feet (procured from a company), section and stain the sections with the basic H&E, modified Hart's, Picro sirius red and such like. Any help would be greatly appreciated. Thanks Yak-Nam On Fri, Apr 10, 2009 at 1:11 PM, Woodward, Denise wrote: > I believe there is a computerized image file of an entire male and a > female human body somewhere on the WWW. Maybe NIH?? These were whole mount > histologic sections. Don't know if the plane is correct for your needs with > regard to the sections of feet. News about it came out about 4-5 years ago. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin > Sent: Wednesday, April 08, 2009 12:07 AM > To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Whole human feet > > No offense, dude, but GROSS. > > That's probably why nobody has bitten yet, in regards to this query. > > > > > ________________________________ > From: Yak-Nam Wang > To: histonet@lists.utsouthwestern.edu > Sent: Monday, April 6, 2009 8:04:54 PM > Subject: [Histonet] Whole human feet > > Hello all, > > If possible, we would like to obtain histological sections of adult human > feet (plane of the anterior-posterior surface). Does anyone know of > labs/groups that have done this or something similar? > > Thank you for your help > Yak-Nam Wang > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Apr 10 15:41:55 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Apr 10 15:42:11 2009 Subject: [Histonet] Whole human feet In-Reply-To: Message-ID: Virtual autopsy web site? They have the whole body ct scanned and sliced. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Woodward, Denise Sent: Friday, April 10, 2009 3:11 PM To: 'Bernie Taupin'; Yak-Nam Wang; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Whole human feet I believe there is a computerized image file of an entire male and a female human body somewhere on the WWW. Maybe NIH?? These were whole mount histologic sections. Don't know if the plane is correct for your needs with regard to the sections of feet. News about it came out about 4-5 years ago. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 08, 2009 12:07 AM To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Whole human feet No offense, dude, but GROSS. That's probably why nobody has bitten yet, in regards to this query. ________________________________ From: Yak-Nam Wang To: histonet@lists.utsouthwestern.edu Sent: Monday, April 6, 2009 8:04:54 PM Subject: [Histonet] Whole human feet Hello all, If possible, we would like to obtain histological sections of adult human feet (plane of the anterior-posterior surface). Does anyone know of labs/groups that have done this or something similar? Thank you for your help Yak-Nam Wang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ynwang <@t> u.washington.edu Fri Apr 10 15:48:19 2009 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Fri Apr 10 15:48:24 2009 Subject: [Histonet] Whole human feet In-Reply-To: <654899.71755.qm@web65711.mail.ac4.yahoo.com> References: <33b7cf2b0904101326l352ce13dm7c1185e2c0329a7d@mail.gmail.com> <654899.71755.qm@web65711.mail.ac4.yahoo.com> Message-ID: <33b7cf2b0904101348w1ea35c0cl1e39cc2fd261511@mail.gmail.com> Thanks Rene, I have heard of the sliding microtomes and a colleague thought that as long as I could fix and decalcify, it might be possible with one of these. I fear I may have to pre-cut the foot in to manageable portions before processing. Yes, if I find out how to do it I will definitely let you know! Yak-Nam On Fri, Apr 10, 2009 at 1:34 PM, Rene J Buesa wrote: > As far as I know such a large thin section that also involves decalcifying > hard bones has never been published. In the past E. Leitz (now Leica) > manufactured sliding microtomes for large specimens but were used mostly to > prepare thin brain sections embedded in celloidin but not for what you would > like to do. > If you are able to do it, for sure we all would like to know. > Ren? J. > > --- On *Fri, 4/10/09, Yak-Nam Wang * wrote: > > From: Yak-Nam Wang > Subject: Re: [Histonet] Whole human feet > To: "Woodward, Denise" > Cc: "histonet@lists.utsouthwestern.edu" > > Date: Friday, April 10, 2009, 4:26 PM > > > Denise, > > Thanks, someone else pointed out these images and they are indeed in the > correct plane (http://www.nlm.nih.gov/research/visible/image/feet.jpg). I > think these are 1 mm sections from a frozen body, unstained. We were > actually hoping that we could process feet (procured from a company), > section and stain the sections with the basic H&E, modified Hart's, > Picro > sirius red and such like. > > Any help would be greatly appreciated. > Thanks > Yak-Nam > > On Fri, Apr 10, 2009 at 1:11 PM, Woodward, Denise > wrote: > > > I believe there is a computerized image file of an entire male and a > > female human body somewhere on the WWW. Maybe NIH?? These were whole > mount > > histologic sections. Don't know if the plane is correct for your needs > with > > regard to the sections of feet. News about it came out about 4-5 years > ago. > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin > > Sent: Wednesday, April 08, 2009 12:07 AM > > To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Whole human feet > > > > No offense, dude, but GROSS. > > > > That's probably why nobody has bitten yet, in regards to this query. > > > > > > > > > > ________________________________ > > From: Yak-Nam Wang > > To: histonet@lists.utsouthwestern.edu > > Sent: Monday, April 6, 2009 8:04:54 PM > > Subject: [Histonet] Whole human feet > > > > Hello all, > > > > If possible, we would like to obtain histological sections of adult human > > feet (plane of the anterior-posterior surface). Does anyone know of > > labs/groups that have done this or something similar? > > > > Thank you for your help > > Yak-Nam Wang > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From bernie_taupin <@t> ymail.com Fri Apr 10 16:20:34 2009 From: bernie_taupin <@t> ymail.com (Bernie Taupin) Date: Fri Apr 10 16:21:15 2009 Subject: [Histonet] HISTOS 5 processor Message-ID: <888372.81380.qm@web43508.mail.sp1.yahoo.com> Hi Ann, How are things down there in "Flodrida"? Glad you "get some money" to buy something form "Smilestone"! Love, Bernie Taupin _____________________________ Hi, all We get some money recently and want to spend it on the HISTOS 5 proceesor from Smilestone. But we are not sure whether it is worth purchasing. Does anyone here have any experiences or suggestions of it? Thank you, Ann Fu University of Flodrida From dlschneider <@t> gmail.com Fri Apr 10 16:24:06 2009 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Fri Apr 10 16:24:10 2009 Subject: [Histonet] Histotech jobs in West Texas Message-ID: <1085e7000904101424s43dad971p90fb3afa15e2f2e6@mail.gmail.com> I'm a pathologist in a group practice in West Texas. We're seeking a histology supervisor, as well as skilled histotechs at bench level, for long term employment. If you're interested, or know someone who might be, drop me an email and we'll discuss the specifics. Daniel Schneider, MD (No offense, but I'm not a headhunter, and I'm not fishing for replies from headhunters either. If I get desperate I know where to find you guys. Thanks!) From dwaugh <@t> kent.edu Fri Apr 10 17:55:14 2009 From: dwaugh <@t> kent.edu (David Waugh) Date: Fri Apr 10 17:55:20 2009 Subject: [Histonet] List abuse In-Reply-To: <888372.81380.qm@web43508.mail.sp1.yahoo.com> References: <888372.81380.qm@web43508.mail.sp1.yahoo.com> Message-ID: <07DC637C-B083-4443-9A22-1B94814D6244@kent.edu> "Bernie", list moderators, and people who are better at tracking things: Looking at the long headers (that contain all the IP addresses of senders/receivers) "Bernie Taupin" emails originate from UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY and an IP address in Brooklyn NYC. Also, one can note, looking at the histonet archives, that "Bernie" and "aa" (in the long header, one can see that "aa" also used the address lactose.intolerant@yahoo.com) use the same IPs and send messages within close proximity of each other. Both senders should clearly be ignored, and maybe someone from UMDNJ could look into the matter. Bernie is most likely in the medical profession, and posts to the list under his or her real name. This is clearly a case of professional misconduct and a misuse of public resources. I am not a IT professional, my analysis could be faulty, but, someone with the proper skills could track this down. -David On Apr 10, 2009, at 5:20 PM, Bernie Taupin wrote: > Hi Ann, > > How are things down there in "Flodrida"? Glad you "get some money" > to buy something form "Smilestone"! > > Love, > Bernie Taupin > > _____________________________ > > Hi, all > > > We get some money recently and want to spend it on the HISTOS 5 > proceesor from Smilestone. But we are not sure whether it is worth > purchasing. Does anyone here have any experiences or suggestions > of it? > > Thank you, > > > Ann Fu > University of Flodrida > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micro <@t> superlink.net Sat Apr 11 20:51:34 2009 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Sat Apr 11 20:51:46 2009 Subject: [Histonet] glassware for sale References: <0E28829006B74240B937902317411A28@shop1e2e996aa5> Message-ID: Are you exempt from the advertising ban on the Histonet? ----- Original Message ----- From: "Cathy Mayton" To: Sent: Friday, April 10, 2009 12:15 PM Subject: [Histonet] glassware for sale I have the following glassware that needs a home Glass beakers 2 1000 ml 5 600 ml 1 400 ml 3 100 ml Plastic cylinders 1 500 ml 1 250 ml 2 100 ml Small glass staining dishes 4 dishes 3 staining racks Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Sun Apr 12 20:18:35 2009 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Apr 12 20:18:40 2009 Subject: [Histonet] glassware for sale Message-ID: <582736990904121818u1fe6b3c8qe9c796cbce287ea9@mail.gmail.com> Markus, I really don't think this would necessarily qualify as "advertising". I have had accidental oversupplies of previously used glassware (and new come to think of it), and needed to find a new home for it. Based on this list of items I really don't think she has a commercial interest in promoting a business of glassware redistribution. I hope this snap to cynical replies is not contagious. We all need to chill a bit. Peace ... REALLY! Amos > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 11 Apr 2009 21:51:34 -0400 > From: "Markus F. Meyenhofer" > Subject: Re: [Histonet] glassware for sale > To: "Cathy Mayton" , > > Message-ID: < DBECEB0E5C71456CBDD9D0340E041F74@DJ4VDH31> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Are you exempt from the advertising ban on the Histonet? > ----- Original Message ----- > From: "Cathy Mayton" > To: > Sent: Friday, April 10, 2009 12:15 PM > Subject: [Histonet] glassware for sale > > > I have the following glassware that needs a home > > Glass beakers > 2 1000 ml > 5 600 ml > 1 400 ml > 3 100 ml > > Plastic cylinders > 1 500 ml > 1 250 ml > 2 100 ml > > Small glass staining dishes > > 4 dishes > 3 staining racks > > Cathy A. Mayton > Wasatch Histo Consultants, Inc. > 775-625-4425 From hilda_1075 <@t> yahoo.com Mon Apr 13 00:27:36 2009 From: hilda_1075 <@t> yahoo.com (shazana hilda) Date: Mon Apr 13 00:27:40 2009 Subject: [Histonet] query regarding silde preparation for LCM Message-ID: <808376.28508.qm@web38808.mail.mud.yahoo.com> Dear Histonetters, i've got few problem regarding the slide preparation for laser microdissection. for your information, i'm using a PEN-membrane from PALM to be used on laser microdissection pressure catapult (LMPC), Ziess. According to the protocol i need to UV the slide prior staining about 30min in order to make it hydrophilic. i've done it. then continue fish the tissue after sectioned.(i'm using polyester wax embedded similar like paraffin embedded jst this is low melting wax for RNA work). then i've heat the slide on slide heater (to dewax) bout 45min at 37celcius. i've tried multiple period but seems like the tissue hard to attach. after dip in 1st 100% ethanol (rehydration step) all tissue detached. if using conventional immunohistochemistry protocol, the slide should be heat bout 1hr at 60celcius.i'm affraid if prolong heat the slide to 1hr at 37celcius will be detrimental to the RNA. This slide will be stained suing Cresyl Violet. Does anyone have any suggestion to solve this matter? Many thanks. Hilda MSc.of Molecular Pathology, Dept. of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. Email: hilda_1075@yahoo.com From kemlo <@t> f2s.com Mon Apr 13 02:18:25 2009 From: kemlo <@t> f2s.com (kemlo) Date: Mon Apr 13 02:18:39 2009 Subject: [Histonet] List abuse In-Reply-To: <07DC637C-B083-4443-9A22-1B94814D6244@kent.edu> References: <888372.81380.qm@web43508.mail.sp1.yahoo.com> <07DC637C-B083-4443-9A22-1B94814D6244@kent.edu> Message-ID: <1FBDA340939C461288A1A653B8478F51@KemloPC> Is he still here? I thought he'd gone, at least the moderator told me so last week; Oh well never mind but I wish people wouldn't reply to his/ her (must be a 'his') as it gets round my outlook rule and he doesn't go to the trash can. Please don't reply to whatever 'he' goads you into; please respect my trashcan!!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Waugh Sent: 10 April 2009 23:55 To: Bernie Taupin; Histonet@lists.utsouthwestern.edu Subject: [Histonet] List abuse "Bernie", list moderators, and people who are better at tracking things: Looking at the long headers (that contain all the IP addresses of senders/receivers) "Bernie Taupin" emails originate from UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY and an IP address in Brooklyn NYC. Also, one can note, looking at the histonet archives, that "Bernie" and "aa" (in the long header, one can see that "aa" also used the address lactose.intolerant@yahoo.com) use the same IPs and send messages within close proximity of each other. Both senders should clearly be ignored, and maybe someone from UMDNJ could look into the matter. Bernie is most likely in the medical profession, and posts to the list under his or her real name. This is clearly a case of professional misconduct and a misuse of public resources. I am not a IT professional, my analysis could be faulty, but, someone with the proper skills could track this down. -David On Apr 10, 2009, at 5:20 PM, Bernie Taupin wrote: > Hi Ann, > > How are things down there in "Flodrida"? Glad you "get some money" > to buy something form "Smilestone"! > > Love, > Bernie Taupin > > _____________________________ > > Hi, all > > > We get some money recently and want to spend it on the HISTOS 5 > proceesor from Smilestone. But we are not sure whether it is worth > purchasing. Does anyone here have any experiences or suggestions > of it? > > Thank you, > > > Ann Fu > University of Flodrida > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jamie.erickson <@t> abbott.com Mon Apr 13 06:56:45 2009 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Mon Apr 13 06:56:56 2009 Subject: [Histonet] Re: Whole human feet- Cryomacrotome In-Reply-To: Message-ID: Hi All, It would not be a paraffin sections but you could do a whole human foot with a cryomacrotome (frozen). It can section a small monkey (sagittally) so I think a human foot would be not a problem. There are contract labs (Convance and Quest Pharmaceuticals that do contract whole body work you could contract them. For more information check out the societies web page @ http://www.autoradiography.net/index.html Jamie _______________________________ Jamie Erickson Sr. Research Associate II M.S. HTL (ASCP) Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From relia1 <@t> earthlink.net Mon Apr 13 08:52:02 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Apr 13 08:52:10 2009 Subject: [Histonet] RELIA Job Alert Lead Histotech needed in Springfield MA - In case you missed it over the holiday weekend. Message-ID: Hi Histonetters! I am reposting this in case you missed it over the holiday weekend I hope you are having a great day. I have a new opportunity I want to tell you about! I am working with a premier client in Springfield, MA. My client is in need of a lead histotechnologist for a busy community surgical pathology lab. Knowledge of routine histology and immunohistochemistry is required. Knowledge of fluorescence and chromogenic in-situ hybridization techniques is a plus. In addition the ability to direct and support the work of other histotechnologists is required. This is a permanent fulltime M-F day shift position. My client offers a laboratory equipped with state of the art instrumentation, a competitive salary and excellent benefits. If you or someone you know might be interested please contact me. I can be reached toll free at 866-607-3542 or relia1@earthlink.net Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia From DixonM <@t> vetmed.ufl.edu Mon Apr 13 08:55:43 2009 From: DixonM <@t> vetmed.ufl.edu (MaryAnn Dixon) Date: Mon Apr 13 08:55:48 2009 Subject: [Histonet] travelling histotechs Message-ID: <530D827EC657DE418C3572ADD63FCDC32496DA@EXGVMCNETWORK.vetmed.ufl.edu> Hi histonetters, I had some questions about travelling and would like to be contacted offline from a few of you experts that have done this or are currently buzzing around the country. Thanks! MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 352-2235 Ext. 4517 From gokland <@t> ameripath.com Mon Apr 13 10:00:01 2009 From: gokland <@t> ameripath.com (Okland, Gloria) Date: Mon Apr 13 10:00:31 2009 Subject: [Histonet] processing artifact Message-ID: <6240949703BA0B4FB724CF6B404BC3F10304442597@MWNMAIL00.ameripath.local> We are seeing this opaque artifact in some tissues. It can affect one biopsy but not the two others in the same cassette. It is very random, appears as this white haze, obscuring nuclear detail, making the slides very difficult to read. Thinking it was water in the processor, I did a test run over the weekend and everything looks great. No reagents had been changed or adjustments made for the test run. Any suggestions of what else this could be and a remedy would be greatly appreciated!!.. Thank you. Gloria Gloria Okland AmeriPath | Histology Please think about resource conservation before you print this message CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Mon Apr 13 10:36:28 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 13 10:36:32 2009 Subject: [Histonet] processing artifact In-Reply-To: <6240949703BA0B4FB724CF6B404BC3F10304442597@MWNMAIL00.ameripath.local> Message-ID: <991623.14237.qm@web65712.mail.ac4.yahoo.com> Gloria: Frequently the problems that appear in the sections are attributed to processing defects when they usually originate before processing, during fixation. Small pieces of tissue left to dry before being placed in the fixative. You write that sometimes happens to one biopsy in a group of several so consider the following: 4 biopsies are going to be taken and all are placed over gauze before being placed in the fixative. The one that was taken first was the one that had the greater chance to dry out totally or partially. The others stayed on the gauze less time, the one taken the first being the one that dried the most, the one that fixed less and the one perhaps that appears now with poor staining. Check how those biopsy cases that you have problem now were taken, perhaps then you can find an explanation. Hope this will help you Ren? J. --- On Mon, 4/13/09, Okland, Gloria wrote: From: Okland, Gloria Subject: [Histonet] processing artifact To: "histonet@lists.utsouthwestern.edu" Date: Monday, April 13, 2009, 11:00 AM We are seeing this opaque artifact in some tissues. It can affect one biopsy but not the two others in the same cassette. It is very random, appears as this white haze, obscuring nuclear detail, making the slides very difficult to read. Thinking it was water in the processor, I did a test run over the weekend and everything looks great. No reagents had been changed or adjustments made for the test run. Any suggestions of what else this could be and a remedy would be greatly appreciated!!.. Thank you. Gloria Gloria Okland AmeriPath | Histology Please think about resource conservation before you print this message CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mlbukhar <@t> ucalgary.ca Mon Apr 13 10:41:38 2009 From: mlbukhar <@t> ucalgary.ca (maureen bukhari) Date: Mon Apr 13 10:41:58 2009 Subject: [Histonet] Xylene substitute Message-ID: <001c01c9bc4e$55e3c030$01ab4090$@ca> Does anyone out there in histoland use Formula 83 by CBG Biotech as a replacement for xylene. If so, how does it compare monetarily and as a clearant? Maureen Bukhari MLT (CSMLS) Histology Technologist Lab 2B26A HRIC Building, 3330 Hospital Drive, NW, University of Calgary Faculty of Veterinary Medicine Calgary, Alberta T2N 4N1 Phone:403-210-6524 e-mail: mlbukhar@ucalgary.ca From Brenda <@t> nsh.org Mon Apr 13 10:50:54 2009 From: Brenda <@t> nsh.org (Brenda Royce) Date: Mon Apr 13 10:51:07 2009 Subject: [Histonet] NSH Summer Symposium Invitation Message-ID: Registration is now open for our 2nd NSH Summer Symposium. This year's 2 day event will be held in the exciting city of Las Vegas, Nevada on June 15-16, 2009. Don't miss this cost effective opportunity to earn up to 10 contact hours while sharing ideas with other attendees in your field. Seats are filling up, so register today! Please visit our website for more information at www.nsh.org . If you have any questions, feel free to call the office at 443-535-4060 or e-mail histo@nsh.com We hope to see your there! From lblazek <@t> digestivespecialists.com Mon Apr 13 10:57:02 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Apr 13 10:52:01 2009 Subject: [Histonet] Xylene substitute In-Reply-To: <001c01c9bc4e$55e3c030$01ab4090$@ca> References: <001c01c9bc4e$55e3c030$01ab4090$@ca> Message-ID: <5A2BD13465E061429D6455C8D6B40E3908778D1586@IBMB7Exchange.digestivespecialists.com> Maureen, I have used Formula 83 from CBG for several years now and have no complaints about the product. I use it in processing, staining and on my coverslipper. I also recycle it. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of maureen bukhari Sent: Monday, April 13, 2009 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene substitute Does anyone out there in histoland use Formula 83 by CBG Biotech as a replacement for xylene. If so, how does it compare monetarily and as a clearant? Maureen Bukhari MLT (CSMLS) Histology Technologist Lab 2B26A HRIC Building, 3330 Hospital Drive, NW, University of Calgary Faculty of Veterinary Medicine Calgary, Alberta T2N 4N1 Phone:403-210-6524 e-mail: mlbukhar@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Mon Apr 13 11:12:46 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Mon Apr 13 11:13:04 2009 Subject: [Histonet] processing artifact Message-ID: <49E32C3E020000EE00028299@smtp-gw.hurleymc.com> Gloria, We have had issues with our small biopsies in the past also. We actually had several things going on that we were able to solve with a little help from our friends in the field! Rene's explaination is very valid as it was also one of our issues. Since your processing schedule/rotation sounds good, have you considered the type of cassettes you are using? We were using Histoscreen Cassettes and found that they processed good some days, and bad other times........and this was with multiple biopsies in the same cassette. We found that during the processing, air bubbles within the cassettes were causing the incomplete processing. Those little screens, while very handy in containing the specimen, created air bubbles when the solutions were pumped in/out. If one of the small biopsies got caught in a air bubble, it missed that part of the processing. That took us a while to figure that one out. Now that we've omitted them, our inconsistant processing is solved. We had little raisins for nuclei. Not good!! Hope this helped. Let us know what you find out...... Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810-257-9948 fax: 810-762-7082 >>> "Okland, Gloria" 04/13/09 11:00 AM >>> We are seeing this opaque artifact in some tissues. It can affect one biopsy but not the two others in the same cassette. It is very random, appears as this white haze, obscuring nuclear detail, making the slides very difficult to read. Thinking it was water in the processor, I did a test run over the weekend and everything looks great. No reagents had been changed or adjustments made for the test run. Any suggestions of what else this could be and a remedy would be greatly appreciated!!.. Thank you. Gloria Gloria Okland AmeriPath | Histology Please think about resource conservation before you print this message CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Apr 13 11:18:36 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 13 11:18:40 2009 Subject: [Histonet] Xylene substitute In-Reply-To: <001c01c9bc4e$55e3c030$01ab4090$@ca> Message-ID: <348777.35154.qm@web65713.mail.ac4.yahoo.com> Formula 83 costs 33% less than xylene (Formula 83 = 0.67 xylene). ren? J. --- On Mon, 4/13/09, maureen bukhari wrote: From: maureen bukhari Subject: [Histonet] Xylene substitute To: histonet@lists.utsouthwestern.edu Date: Monday, April 13, 2009, 11:41 AM Does anyone out there in histoland use Formula 83 by CBG Biotech as a replacement for xylene. If so, how does it compare monetarily and as a clearant? Maureen Bukhari MLT (CSMLS) Histology Technologist Lab 2B26A HRIC Building, 3330 Hospital Drive, NW, University of Calgary Faculty of Veterinary Medicine Calgary, Alberta T2N 4N1 Phone:403-210-6524 e-mail: mlbukhar@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From d-emge <@t> northwestern.edu Mon Apr 13 11:31:50 2009 From: d-emge <@t> northwestern.edu (Donna J Emge) Date: Mon Apr 13 11:32:01 2009 Subject: [Histonet] Need Quotes for IHC Open System Autostainer ASAP Message-ID: <000501c9bc55$589bd720$09d38560$@edu> Ok all you sales reps. I need quotes early this week!! for an open system immunohistochemistry autostainer. This is for a very small research lab doing mainly mouse and rat histology. Ideally I would also like it to do in situ hybridization, but systems without this are fine. I also need to have an idea of some of the hidden costs. Big factors in the decision of which system to buy will be: functionality, price, customer service and support, ease of use, hidden costs, and is it a truly open system. So far the Biocare Intellipath is at the top of the list because of the satisfaction of several people on the Histonet. If anyone wants to share more information about their system and why they love it or hate it I would be glad to have that information also. I did take a look at several of the archives recently, but love the detailed information you Histonetters provide. Donna J. Emge, Phenotyping Core Manager Northwestern University Olson Building room 8-335 710 North Fairbanks Court Chicago, IL 60611 d-emge@northwestern.edu Cell 708-715-5720 Lab 312-503-2679 From fudo <@t> ufl.edu Mon Apr 13 11:38:42 2009 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Mon Apr 13 11:38:47 2009 Subject: [Histonet] HISTOS 5 processor-Thank you Message-ID: <1182033601.82331239640722545.JavaMail.osg@osgjas01.cns.ufl.edu> Hi, all Thank you very much to the people who responded to my email and gave me a lot of suggestions & information. I will work on it. Thank you again and have a nice Monday, Ann On Fri Apr 10 17:20:34 EDT 2009, Bernie Taupin wrote: > Hi Ann, > > How are things down there in "Flodrida"? Glad you "get some > money" to buy something form "Smilestone"! > > Love, > Bernie Taupin > > _____________________________ > > Hi, all > > > We get some money recently and want to spend it on the HISTOS 5 > proceesor from Smilestone. But we are not sure whether it is > worth purchasing. Does anyone here have any experiences or > suggestions of it? > > Thank you, > > > Ann Fu > University of Flodrida > > > From jcline <@t> wchsys.org Mon Apr 13 12:05:37 2009 From: jcline <@t> wchsys.org (Joyce Cline) Date: Mon Apr 13 12:05:40 2009 Subject: [Histonet] Xylene substitute In-Reply-To: <001c01c9bc4e$55e3c030$01ab4090$@ca> Message-ID: I have used Formula 83 for 4 years. I use it in the processor, stainer and for coverslipping (by hand). The paths like it and we like it. We also recycle it. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of maureen bukhari Sent: Monday, April 13, 2009 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene substitute Does anyone out there in histoland use Formula 83 by CBG Biotech as a replacement for xylene. If so, how does it compare monetarily and as a clearant? Maureen Bukhari MLT (CSMLS) Histology Technologist Lab 2B26A HRIC Building, 3330 Hospital Drive, NW, University of Calgary Faculty of Veterinary Medicine Calgary, Alberta T2N 4N1 Phone:403-210-6524 e-mail: mlbukhar@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From mweirauch <@t> crittenton.com Mon Apr 13 10:58:10 2009 From: mweirauch <@t> crittenton.com (Maray Weirauch) Date: Mon Apr 13 12:14:32 2009 Subject: [Histonet] Looking for Cerner users Message-ID: Our histology department currently uses Cerner Millenium Pathnet. Our specimens have always arrived with hard copy requisitions, but our surgical departments are installing the Cerner surgical application - Surginet. Is anyone familiar with using this system to electronically 'order' the pathology specimens? We want to go paperless, but it seems that the process for surgery to enter pre- and post- op diagnosis and specimen information gets rather cumbersome to use. When we ask Cerner what other places use both Pathnet and Surginet, we can't get an answer. We really don't want to re-work the wheel- Anyone have experience with this? Thanks in advance! From mtighe <@t> trudeauinstitute.org Mon Apr 13 12:31:32 2009 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Mon Apr 13 12:31:56 2009 Subject: [Histonet] BSL3 clean-up and Paraffin odor Message-ID: <49E33EA6.26E4.00EE.0@trudeauinstitute.org> We have a BSL3 laboratory with a cryostat (TB research). The cryostat user asked me for the best method to decontaminate the cryostat. My suggestion was to clean the cryostat (while cold) with 95% ethanol followed by absolute ethanol after wards. Then to defrost the cryostat and clean with a stronger disinfectant such as amphyl/lysol. Does this sound reasonable to those of you who work with BSL3 level pathogens? Is there any thing you might add to this protocol? Second, someone asked me if smelling the paraffin (not xylene) was hazardous to your health. I have never been concerned about the smell of paraffin alone but since it was asked of me I thought I would see if any of you have had to worry about this? Thanks!! Mike From jrobertson <@t> pathologysciences.com Mon Apr 13 12:51:46 2009 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Mon Apr 13 12:51:51 2009 Subject: [Histonet] Xylene substitute In-Reply-To: <001c01c9bc4e$55e3c030$01ab4090$@ca> References: <001c01c9bc4e$55e3c030$01ab4090$@ca> Message-ID: <518CD6920AA7154193CBE5977CD880733A8942@psmgsrv2.PSMG.local> We use it in our processors and it works wonderfully. It does have a strong odor and that's why we only use it in our processors. It also distills great. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Histology Day Supervisor 183 E. 8th Ave. Chico, CA 95926 530-891-6244 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of maureen bukhari Sent: Monday, April 13, 2009 8:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene substitute Does anyone out there in histoland use Formula 83 by CBG Biotech as a replacement for xylene. If so, how does it compare monetarily and as a clearant? Maureen Bukhari MLT (CSMLS) Histology Technologist Lab 2B26A HRIC Building, 3330 Hospital Drive, NW, University of Calgary Faculty of Veterinary Medicine Calgary, Alberta T2N 4N1 Phone:403-210-6524 e-mail: mlbukhar@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Mon Apr 13 13:16:52 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Mon Apr 13 13:16:57 2009 Subject: [Histonet] Re: Xylene substitute Message-ID: You can view the MSDS for CBG Biotech's "Formula 83" xylene substitute at www.cbgbiotech.com/msds/MSDS-Formula83071007.pdf It's described as a "naphthenic hydrocarbon blend". I'd note the low flash point (7 C, 45 F), considerably lower than xylene's. Other aliphatic or naphthenic (cycloalkane) mixtures offered as clearing agents have considerably higher flash points. Remember that if you want to recover it by distillation, you must make sure that your still has a distillation routine for it, and you cannot change clearing agents without changing the distillation routine, nor can you mix the solvents and recover them by distillation. If your lab manager shares cost information with you (in my experience most do not) then cost will certainly be a consideration, if it's indeed cheaper than xylene. According to the MSDS, disposal is done like any other aliphatic or naphthenic clearing agent. Bob Richmond Samurai Pathologist Knoxville TN From alyssa <@t> alliedsearchpartners.com Mon Apr 13 14:27:07 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Mon Apr 13 14:27:15 2009 Subject: [Histonet] Position In Corpus Christi, TX Message-ID: Hello to all. I have a position in *Corpus Christi**, **TX** area. *If this is not the job for you, however, you are interested in exploring new opportunities please send me a copy of your updated resume. Also, we offer to pay you up to $1000 for any referral that we place in a position, so please forward this to anyone who seems fit for this position and have them contact me right away! *Position: Histology Technician* Department:Histology Specialty Area:Allied Health Supervisor's Title:Histology Manager Work Schedule:FT Early Morning* MONDAY-FRIDAY WITH WEEKEND ROTATION* ------------------------------ Experience Required: ? Approximately 6moths-1 year on-the-job experience necessary Job Description: ? Performs various histological procedures to prepare and process tissue specimens for examination by pathologist. ? Provides information for diagnosis and treatment by conducting histological procedures following policies and procedures. Education: ? HT certification by Board of Registry of the American Society of Clinical Pathologists. *Benefits: * ? 1. competitive compensation package, compensation continuously reviewed and updated. Relocation Assistance. Medical Benefits, and much more! -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 From asachau <@t> titanmed.com Mon Apr 13 16:03:33 2009 From: asachau <@t> titanmed.com (April Sachau) Date: Mon Apr 13 16:05:07 2009 Subject: [Histonet] Permanent Histology positions available in New England!!! In-Reply-To: <33b7cf2b0904101326l352ce13dm7c1185e2c0329a7d@mail.gmail.com> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F701E1A0C5@titansbs1.corp.titanmed.com> Hello Histoland! I have 2 permanent (routine) Histology positions available at this time. One is a rotating shift, the other 4am-12pm. If you are interested, or know someone who maybe, please contact me at asachau@titanmed.com. I look forward to hearing from you!!! :) April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From indytreegers <@t> sbcglobal.net Tue Apr 14 08:40:24 2009 From: indytreegers <@t> sbcglobal.net (Lyn) Date: Tue Apr 14 08:40:29 2009 Subject: [Histonet] indytreegers@sbcglobal.net has invited you to have a 3D avatar chat Message-ID: <200904141340.n3EDeOVe005793@AF001299.prod.imvu.com> From: Lyn Avatar: Guest_LynTreeger To: Histo Hey Histo,Lyn has added you as a friend on IMVU. Is Lyn your friend? Yes   No Please respond or Lyn may think you said no :) IMVU is the world's greatest 3D chat! Dress up your Avatar with 3D clothes. Chat with your friends & meet new ones. Decorate your own 3D Room with furniture . FREE to download & use! http://www.imvu.com Copyright © 2009 IMVU, Inc. 411 High Street, Palo Alto, CA 94301. This email was sent via IMVU by Lyn (indytreegers@sbcglobal.net) to histonet@lists.utsouthwestern.edu. If you want to prevent any future emails from IMVU, you can remove yourself by pointing your web browser to http://www.imvu.com/catalog/web_nonregisteredoptout.php?code=c4aa48&email=histonet@lists.utsouthwestern.edu. Your unsubscribe confirmation code is c4aa48 From micropathlabs <@t> yahoo.com Tue Apr 14 08:44:30 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Tue Apr 14 08:44:34 2009 Subject: [Histonet] IMP-3 Protocol Message-ID: <472311.1469.qm@web57806.mail.re3.yahoo.com> Hi all. Has anyone worked up the IMP-3 antibody for the Benchmark? I am interested in a dilution that works?with the I-view detection if you'd be willing to share. Thank you! ? Sheila Haas Laboratory Supervisor Micro Path Laboratories From shimjudy <@t> gmail.com Tue Apr 14 08:54:07 2009 From: shimjudy <@t> gmail.com (Yeonju Shim) Date: Tue Apr 14 08:54:11 2009 Subject: [Histonet] Embedding Stamp??? Message-ID: <7c363b8a0904140654i5b514858na8950f4051f01e46@mail.gmail.com> Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy From jqb7 <@t> cdc.gov Tue Apr 14 08:56:55 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Apr 14 08:57:11 2009 Subject: [Histonet] Embedding Stamp??? In-Reply-To: <7c363b8a0904140654i5b514858na8950f4051f01e46@mail.gmail.com> References: <7c363b8a0904140654i5b514858na8950f4051f01e46@mail.gmail.com> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF0595@LTA3VS011.ees.hhs.gov> It's called a tamper and I know Fisher sells them.....search embedding tamper on the web site. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yeonju Shim Sent: Tuesday, April 14, 2009 9:54 AM To: histonet; histonet-owner@lists.utsouthwestern.edu Subject: [Histonet] Embedding Stamp??? Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rfields <@t> gidocs.net Tue Apr 14 08:56:49 2009 From: rfields <@t> gidocs.net (Rosa Fields) Date: Tue Apr 14 08:59:31 2009 Subject: [Histonet] Embedding Stamp??? References: <7c363b8a0904140654i5b514858na8950f4051f01e46@mail.gmail.com> Message-ID: <07732CE52EC3174AB891DE1C62DB4D8F6F95EF@GIEXCHANGE.gidocs.net> Look at your local hardware store for different size bolts.. you should be able to find a nice range of sizes to suit your needs.. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yeonju Shim Sent: Tuesday, April 14, 2009 8:54 AM To: histonet; histonet-owner@lists.utsouthwestern.edu Subject: [Histonet] Embedding Stamp??? Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Apr 14 09:00:56 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Apr 14 09:01:03 2009 Subject: [Histonet] Embedding Stamp??? In-Reply-To: <7c363b8a0904140654i5b514858na8950f4051f01e46@mail.gmail.com> References: <7c363b8a0904140654i5b514858na8950f4051f01e46@mail.gmail.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA5477BB5@ITSSSXM01V6.one.ads.che.org> They are called tampers and are available at Sakura - 1551 and 1552. They are also available through Cardinal, but I'm not sure the item numbers for Cardinal. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yeonju Shim Sent: Tuesday, April 14, 2009 9:54 AM To: histonet; histonet-owner@lists.utsouthwestern.edu Subject: [Histonet] Embedding Stamp??? Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From rjbuesa <@t> yahoo.com Tue Apr 14 09:03:34 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 14 09:03:38 2009 Subject: [Histonet] Embedding Stamp??? In-Reply-To: <7c363b8a0904140654i5b514858na8950f4051f01e46@mail.gmail.com> Message-ID: <466147.26545.qm@web65701.mail.ac4.yahoo.com> Some people call it a "thumper" (I do not like the name at all) and others call it a tissue "pressing tool" or a tissue "flattening tool". Always made of aluminum with a "T" shape. Ren? J. --- On Tue, 4/14/09, Yeonju Shim wrote: From: Yeonju Shim Subject: [Histonet] Embedding Stamp??? To: "histonet" , histonet-owner@lists.utsouthwestern.edu Date: Tuesday, April 14, 2009, 9:54 AM Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Tue Apr 14 09:08:11 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Tue Apr 14 09:08:14 2009 Subject: [Histonet] Embedding Stamp??? In-Reply-To: <466147.26545.qm@web65701.mail.ac4.yahoo.com> Message-ID: <60463118.78501239718091193.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> It is also called a "tamper".? Pam Marcum ----- Original Message ----- From: "Rene J Buesa" To: "histonet" , histonet-owner@lists.utsouthwestern.edu, "Yeonju Shim" Sent: Tuesday, April 14, 2009 10:03:34 AM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] Embedding Stamp??? Some people call it a "thumper" (I do not like the name at all) and others call it a tissue "pressing tool" or a tissue "flattening tool". Always made of aluminum with a "T" shape. Ren? J. --- On Tue, 4/14/09, Yeonju Shim wrote: From: Yeonju Shim Subject: [Histonet] Embedding Stamp??? To: "histonet" , histonet-owner@lists.utsouthwestern.edu Date: Tuesday, April 14, 2009, 9:54 AM Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From indytreegers <@t> sbcglobal.net Tue Apr 14 09:08:54 2009 From: indytreegers <@t> sbcglobal.net (indytreegers@sbcglobal.net) Date: Tue Apr 14 09:08:57 2009 Subject: [Histonet] "Avatar" Message--Apology to List Message-ID: <277472.32943.qm@web81906.mail.mud.yahoo.com> Hello Everyone, ? This crazy program got ahold of my email list and sent this mess out.? I apologize to one and all for the inconvenience!! ? Sorry, ? Lyn From gmartin <@t> marshallmedical.org Tue Apr 14 09:12:23 2009 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Tue Apr 14 09:12:26 2009 Subject: [Histonet] Embedding Stamp??? In-Reply-To: <7c363b8a0904140654i5b514858na8950f4051f01e46@mail.gmail.com> References: <7c363b8a0904140654i5b514858na8950f4051f01e46@mail.gmail.com> Message-ID: <6ED9D4252F278841A0593D3D788AF24C0505011F@mailsvr.MARSHMED.local> Go to a Pipe shop and look into tobacco tampers. Very useful ... they have a longer handle and don't seem quite as hot as the square Histo tamper. Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yeonju Shim Sent: Tuesday, April 14, 2009 6:54 AM To: histonet; histonet-owner@lists.utsouthwestern.edu Subject: [Histonet] Embedding Stamp??? Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lac65 <@t> email.med.yale.edu Tue Apr 14 09:20:40 2009 From: lac65 <@t> email.med.yale.edu (Lori Charette) Date: Tue Apr 14 09:20:49 2009 Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 22 In-Reply-To: <200904091701.n39H1W3e015535@mr4.its.yale.edu> References: <200904091701.n39H1W3e015535@mr4.its.yale.edu> Message-ID: <49E49BB8.9090303@email.med.yale.edu> Give me a shout in regards to cell pellets & TMA's. There are several methods and some are less expensive then others. Have a good one. Lori Beecher is usually difficult to deal with.histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: TMA for cell pellets? (Bernie Taupin) > 2. To all spam gourmets (V. Neubert) > 3. RE: I recommend NOT opening that Attachment: (Hugh Luk) > 4. RE: TMA for cell pellets? (Hartson, Louise) > 5. Re: Formula 83 users? (Rene J Buesa) > 6. Re: Negative IHC controls (Rene J Buesa) > 7. RE: Formula 83 users? (Blazek, Linda) > 8. Re: Spyware from an Attachment: (Geoff McAuliffe) > 9. Formula 83-Thank you! (Jacqueline Farnsworth) > 10. HISTOS 5 processor (FU,DONGTAO) > 11. Position Open In Mass (Alyssa Peterson) > 12. Re: HISTOS 5 processor (Robert Schoonhoven) > 13. Travelling Histotechs (Robert Schoonhoven) > 14. Regional Sales Manager position- West Coast > (Kris.Caldwell@leica-microsystems.com) > 15. New lab, need some info (Putnam, Jodi) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 8 Apr 2009 22:50:26 -0700 (PDT) > From: Bernie Taupin > Subject: Re: [Histonet] TMA for cell pellets? > To: Thom Jensen , > louise_hartson@urmc.rochester.edu, histonet@lists.utsouthwestern.edu > Message-ID: <338565.20208.qm@web43513.mail.sp1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Recently, someone mentioned putting a library of protocols online, perhaps in the HistoNet archives. I'd like to second that emotion if its possible to make happen... What a great resource that would be! > > > > > ________________________________ > From: Thom Jensen > To: louise_hartson@urmc.rochester.edu; histonet@lists.utsouthwestern.edu > Sent: Thursday, April 9, 2009 1:27:18 AM > Subject: [Histonet] TMA for cell pellets? > > > > Have you tried to embed your cell pellets into a tissue microarray? Two companies I would recommend: > www.arraymold.com > www.beecherinstruments.com > > Two of the best TMA products on the market. > > > cheers, > > >> Date: Thu, 2 Apr 2009 12:05:39 -0400 >> From: Louise_Hartson@URMC.Rochester.edu >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Embedding cell pellets >> >> >> I am looking for a protocol for embedding cell pellets in paraffin. >> Thanks, >> Louise >> >> Louise Hartson, BA >> Senior Technical Associate >> University of Rochester >> Louise_Hartson@URMC.Rochester.edu >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _________________________________________________________________ > Hotmail? is up to 70% faster. Now good news travels really fast. > http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 2 > Date: Thu, 09 Apr 2009 10:07:54 +0200 > From: "V. Neubert" > Subject: [Histonet] To all spam gourmets > To: histonet@lists.utsouthwestern.edu > Message-ID: <49DDACDA.8040503@vneubert.com> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Please, stop spamming this list. > Comment if you know something useful. Otherwise keep it. > I don't mind what you had for breakfast in June 1st last year or your > opinion on my clothes' colour. > > Believe it or not, there IS a real life ( link: > http://en.wikipedia.org/wiki/Real_life_(reality) ), and maybe you should > check it out - it has way more to offer than waiting for responses to > mock about. > > Thanks. > Valentin > > PS: What about a moderated board? Every inappropriate comment on HISTO > TOPIC could be moved or even deleted, except in the spamming forum which > can be found on nearly every board on the net. > > > > ------------------------------ > > Message: 3 > Date: Wed, 8 Apr 2009 22:13:22 -1000 > From: Hugh Luk > Subject: RE: [Histonet] I recommend NOT opening that Attachment: > To: histonet > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > > > > > > > > > > > > > > > > > > > Fellow histology and related fields professionals, > > > > > > > > > > > > I'm not opening this. From a call name of "u know (u_deserve_2_know@yahoo.com)." The last time I opened something like this, from a name like this, I ended up owing money for Viagra treatments for Panda bears or something like that. It may be real, but it probably is spam. There...has been...a lot...of it...recently! > > By the way, there is no such thing as a "Virus-proof LINK!" Not sure? Just Google it. > > If you need Spyware or Antivirus software, check your IT department. Also, CNET has free downloads of AdAware, or AVG antivirus 8. > > And now for a joke (I think we all need it); A physician is doing his rounds and a nurse stops him in the hallway, "Doctor, can you sign these reports?" "Fine" he says and proceeds to pull out a rectal thermometer from his coat pocket. He looks at it in disbelief and exclaims, "Darn it! Some a$$ has my pen!" > > Respectfully, > Hugh Luk, HTL (ASCP) > Cancer Research center of Hawaii > Pathology shared resources lab manager > KP- Hawaii > > > > >> Date: Wed, 8 Apr 2009 21:41:35 -0700 >> From: u_deserve_2_know@yahoo.com >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Spyware from an Attachment: >> >> Dear Listers, >> >> If you ever opened any strange attachments from this list, you should really consider scanning your Hard Disk for spyware. There is a great free anti-spyware program we use, which you can get at http://DELETED<----that is a virus-proof link, by the way. >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _________________________________________________________________ > Quick access to your favorite MSN content and Windows Live with Internet Explorer 8. > http://ie8.msn.com/microsoft/internet-explorer-8/en-us/ie8.aspx?ocid=B037MSN55C0701A > > ------------------------------ > > Message: 4 > Date: Thu, 9 Apr 2009 08:29:23 -0400 > From: "Hartson, Louise" > Subject: RE: [Histonet] TMA for cell pellets? > To: "Bernie Taupin" , "Thom Jensen" > , > Message-ID: > <1089A756B010074CA09E65CD92401608018A8CAC@MEDMAIL.urmc-sh.rochester.edu> > > Content-Type: text/plain; charset="iso-8859-1" > > Thank you to everyone who responded!! I was given the cell pellets already in the matrix so I only had to process!! It was easier than the mouse tissues!! Since we do so few 20-40 at a time I do everything by hand...no automation in our lab!! > > > -----Original Message----- > From: Bernie Taupin [mailto:bernietaupin@ymail.com] > Sent: Thu 4/9/2009 1:50 AM > To: Thom Jensen; Hartson, Louise; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] TMA for cell pellets? > > Recently, someone mentioned putting a library of protocols online, perhaps in the HistoNet archives. I'd like to second that emotion if its possible to make happen... What a great resource that would be! > > > > > ________________________________ > From: Thom Jensen > To: louise_hartson@urmc.rochester.edu; histonet@lists.utsouthwestern.edu > Sent: Thursday, April 9, 2009 1:27:18 AM > Subject: [Histonet] TMA for cell pellets? > > > > Have you tried to embed your cell pellets into a tissue microarray? Two companies I would recommend: > www.arraymold.com > www.beecherinstruments.com > > Two of the best TMA products on the market. > > > cheers, > > >> Date: Thu, 2 Apr 2009 12:05:39 -0400 >> From: Louise_Hartson@URMC.Rochester.edu >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Embedding cell pellets >> >> >> I am looking for a protocol for embedding cell pellets in paraffin. >> Thanks, >> Louise >> >> Louise Hartson, BA >> Senior Technical Associate >> University of Rochester >> Louise_Hartson@URMC.Rochester.edu >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _________________________________________________________________ > Hotmail? is up to 70% faster. Now good news travels really fast. > http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > Message: 5 > Date: Thu, 9 Apr 2009 06:56:21 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Formula 83 users? > To: Histonet , Jacqueline > Farnsworth > Message-ID: <613905.80912.qm@web65715.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Jacqueline: > Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant. > Ren? J. > > --- On Wed, 4/8/09, Jacqueline Farnsworth wrote: > > From: Jacqueline Farnsworth > Subject: [Histonet] Formula 83 users? > To: "Histonet" > Date: Wednesday, April 8, 2009, 12:31 PM > > HI all. > I have searched the Histonet archives and found a lot of very positive reviews > regarding Formula 83. I am wondering if anyone has encountered any issues with > using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there > any miscibility issues? Are you soaking in xylene prior to tape coverslipping > required? Any helpful hints/tips/tricks? > > Thank you in advance. > > > Jacqueline Farnsworth > Anatomic Pathology, Tech III > Foothills Medical Centre > Calgary Laboratory Services > > Ph: 403-944-1578 > Fax: 403-944-4748 > P Please consider the environment before printing this email. > > ________________________________ > This message and any attached documents are only for the use of the intended > recipient(s), are confidential and may contain privileged information. Any > unauthorized review, use, retransmission, or other disclosure is strictly > prohibited. If you have received this message in error, please notify the sender > immediately, and then delete the original message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 6 > Date: Thu, 9 Apr 2009 06:58:45 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Negative IHC controls > To: histonet@lists.utsouthwestern.edu, Sheila Haas > > Message-ID: <292901.13223.qm@web65712.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I used to run a negative control per case or per block within any given case when doing IHC, but not for reagents. > Ren? J. > > --- On Wed, 4/8/09, Sheila Haas wrote: > > From: Sheila Haas > Subject: [Histonet] Negative IHC controls > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, April 8, 2009, 2:57 PM > > I have a question concerning ANP.22570 on the CAP checklist. Could you all > tell me how you are handling > negative controls for IHC staining? The question actually states (and I've > confirmed with CAP) that we should be running two types of negative controls. > One for reagents and one for each antibody in a run. I'd like to know > what the practice is. This seems very costly and time consuming. Thanks in > advance! > > Sheila Haas > Laboratory Supervisor > Micro Path Laboratories > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 7 > Date: Thu, 9 Apr 2009 10:14:48 -0400 > From: "Blazek, Linda" > Subject: RE: [Histonet] Formula 83 users? > To: "'rjbuesa@yahoo.com'" , Histonet > , Jacqueline Farnsworth > > Message-ID: > <5A2BD13465E061429D6455C8D6B40E3908778D156E@IBMB7Exchange.digestivespecialists.com> > > Content-Type: text/plain; charset="iso-8859-1" > > > With all due respect Ren?; I have been using Formula 83 with my coverslipper with no problems at all though have a glass coverslipper not a tape. > Linda > > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Thursday, April 09, 2009 9:56 AM > To: Histonet; Jacqueline Farnsworth > Subject: Re: [Histonet] Formula 83 users? > > Jacqueline: > Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant. > Ren? J. > > --- On Wed, 4/8/09, Jacqueline Farnsworth wrote: > > From: Jacqueline Farnsworth > Subject: [Histonet] Formula 83 users? > To: "Histonet" > Date: Wednesday, April 8, 2009, 12:31 PM > > HI all. > I have searched the Histonet archives and found a lot of very positive reviews > regarding Formula 83. I am wondering if anyone has encountered any issues with > using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there > any miscibility issues? Are you soaking in xylene prior to tape coverslipping > required? Any helpful hints/tips/tricks? > > Thank you in advance. > > > Jacqueline Farnsworth > Anatomic Pathology, Tech III > Foothills Medical Centre > Calgary Laboratory Services > > Ph: 403-944-1578 > Fax: 403-944-4748 > P Please consider the environment before printing this email. > > ________________________________ > This message and any attached documents are only for the use of the intended > recipient(s), are confidential and may contain privileged information. Any > unauthorized review, use, retransmission, or other disclosure is strictly > prohibited. If you have received this message in error, please notify the sender > immediately, and then delete the original message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Thu, 09 Apr 2009 10:11:11 -0400 > From: Geoff McAuliffe > Subject: Re: [Histonet] Spyware from an Attachment: > To: u know > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <49DE01FF.100@umdnj.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Dear List: > > I strongly suggest that you NOT use any links offered by people with no > name and no affiliation. Yes, there are good spyware detection programs > available at no cost (Adaware for example) but I don't think this is one > of them. There are at least 3 potential saboteurs on this list. > > Geoff > > u know wrote: > >> Dear Listers, >> >> If you ever opened any strange attachments from this list, you should really consider scanning your Hard Disk for spyware. There is a great free anti-spyware program we use, which you can get at http://tinyurl.com/ynupj4 <----that is a virus-proof link, by the way. >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > From bill501 <@t> mindspring.com Tue Apr 14 09:28:38 2009 From: bill501 <@t> mindspring.com (Bill B.) Date: Tue Apr 14 09:28:49 2009 Subject: [Histonet] Embedding Stamp??? In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C0505011F@mailsvr.MARSHMED.local> References: <7c363b8a0904140654i5b514858na8950f4051f01e46@mail.gmail.com> <6ED9D4252F278841A0593D3D788AF24C0505011F@mailsvr.MARSHMED.local> Message-ID: Ack! You beat me to it ;-) We use "pipe nails" which come from a local pipe store and are very inexpensive. They work well for prostate and other core biopsies. Bill B. At 7:12 AM -0700 4/14/09, Martin, Gary wrote: >Go to a Pipe shop and look into tobacco tampers. Very useful ... they >have a longer handle and don't seem quite as hot as the square Histo >tamper. From rcharles <@t> state.pa.us Tue Apr 14 09:40:33 2009 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Tue Apr 14 09:40:39 2009 Subject: [Histonet] TBS vs PBS Message-ID: <3809C163DC1DA54AA534B3C7794D07B63298E4C546@ENHBGMBX01.PA.LCL> Hello all, Would proteinase K diluted in PBS be any different then PK diluted in Tris buffer when used for a fluorescent antibody test? I'm having trouble duplicating a published testing method and the only difference is PK diluted in TBS instead of PBS. Thanks in advance for all the wonderful information from all. Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rcharles@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! From relia1 <@t> earthlink.net Tue Apr 14 10:16:23 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Apr 14 10:17:07 2009 Subject: [Histonet] RELIA Histology Job Alert Histology tech needed in Atlanta! Message-ID: Hi Histonetters! I hope everyone is having a great day! I am excited to tell you about a brand new position in a private pathology lab in the Atlanta area. I know this client very well and it is an excellent place to work. They are an established lab with a growing practice. The supervisor is great to work with and the salary and benefits are very competitive for the Atlanta area. This is a permanent full time dayshift position. The schedule is M-F dayshift. My client is in need of an ASCP certified tech with several years of experience. This person will need strong cutting and embedding skills and Ability to work under pressure, attention to detail, excellent fine motor skills for the cutting work required for the tissue, a very ?light touch? when handing delicate biopsies and tissue and strong laboratory skills. While the core schedule is M-F dayshift some flexibility in days and hours is preferred. If you or anyone you know might be interested in this position please contact me. I can be reached toll free at 866-607-3542 or relia1@earthlink.net Have a great day!! I Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net < mailto:relia1@earthlink.net> << http://home.earthlink.net/~relia1>> www.myspace.com/pamatrelia < http://www.myspace.com/pamatrelia> From Vickroy.Jim <@t> mhsil.com Tue Apr 14 10:42:23 2009 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Apr 14 10:44:01 2009 Subject: [Histonet] FORMULA 83 Message-ID: <24A4826E8EF0964D86BC5317306F58A52BB2593441@mmc-mail.ad.mhsil.com> We are experimenting with formula 83 as a clearing agent and chemical in our automated Meisei coverslipper. However it is not compatible with our current mounting medium on the automated coverslipper (TBS shur/mount xylene based. The reason for trying the formula 83 is that we want to completely get rid of xylene, which is now used on the coverslipper. I do not want to however switch from one problem to another since I know that certain mounting mediums do not work well in the automated coverslipper. I believe the "thicker" mounting mediums can cause other issues with the automated coverslipper and that is why we started using the TBS shur/mount xylene based medium in the first place. Does anybody who is using Formula 83 have an alternative that works well in the automated coverslipper and it has approximately the same viscosity as the TBS shur/mount medium? Thanks for your help. Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy.jim@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From integrated.histo <@t> gmail.com Tue Apr 14 10:52:10 2009 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Tue Apr 14 10:52:15 2009 Subject: [Histonet] 5 Reasons Message-ID: <5d9104a30904140852h583d8516h25f3f3e423176afd@mail.gmail.com> Please help me out here.... I am suppose to post 5 Reasons to become a histotech in our lab. It is a competition for lab week. Each department has to post 5 reasons you should work in their department. I am looking for some great answers.... Thanks, Cindy From jlhowery <@t> yrmc.org Tue Apr 14 10:55:41 2009 From: jlhowery <@t> yrmc.org (jeff) Date: Tue Apr 14 10:55:48 2009 Subject: [Histonet] (no subject) Message-ID: <000601c9bd19$76a1ff50$3394640a@yrmc.org> Is anyone using their target retrieval for dako more than 1 time? Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From HornHV <@t> archildrens.org Tue Apr 14 11:04:52 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Apr 14 11:05:54 2009 Subject: [Histonet] decaling bones Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D8313C@EMAIL.archildrens.org> We are having the hardest time decaling bones. Femurs and tibias. Is there such as thing as overdecaling and the bone becoming hard again? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From ian.montgomery <@t> bio.gla.ac.uk Tue Apr 14 11:21:03 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Tue Apr 14 11:21:23 2009 Subject: [Histonet] Blocking Message-ID: <0B6419DD384E4FB9AE0183770A604308@IBLS.GLA.AC.UK> About to use the antibody anti-connexin 43. On the data sheet it tells me to block using 5% milk, so it's casein blocking. In 1992 Dave Tacha published a paper (J. Histotech. 15. 132-137. Casein reduces non-specific background staining in immunolabelling techniques), regarding casein blocking where he recommended using it at 5%. Later however, Mary Vaughan on Histonet suggested reducing the concentration, after a series of experiments, to 0.03% for 30 minutes. What's the current view on casein blocking, concentration, time etc. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From alyssa <@t> alliedsearchpartners.com Tue Apr 14 11:47:14 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Tue Apr 14 11:47:26 2009 Subject: [Histonet] Research/Pharma Position In Mass Message-ID: *Hi,* * * *My name is Alyssa Peterson, and I am the director of Lab/Research recruitment for Allied Search Partners. I wanted to follow up with everyone on Histonet about a position that I am currently setting up interviews for. Please read over the job description and let me know if you are interested in exploring this opportunity and send me an updated resume. If not, we do offer to pay up to $1000 as a referral bonus, so please feel free to forward this to whomever you feel fit. * ** *LOCATION* 20 miles Northwest of Boston, MA *TITLE/POSITION* * * Status: Exempt. Regular, full-time. Work Shift: 8:00am - 5:00pm, M-F, and as required. Flex hours as necessary. Weekly schedule may be Tuesday ? Saturday or Sunday ? Thursday. *QUALIFICATIONS* B.S. in Life Sciences or equivalent experience. Animal handling & restraint experience with both large and small animals required. Must be experienced with all methods of dose administration and biological sample collection techniques in all laboratory species. Self-disciplined, independent worker who can work well on his/her own and with a team. Must possess good time- management skills. Ability to work in a fast-paced environment. Attention to detail. Ability to lift 50 lbs. Must be able to work late nights for study performance and weekends. Prior experience performing necropsies needed. *RESPONSIBILITIES* Conduct In Vivo/Pharma testing under appropriate guidelines (FDA, EPA, GLP, ISO, OECD). Prepare testing solutions, perform dose administration (using IM, IP, ID, IV, SQ, topical, nasal and oral gavage), and perform blood collection on small animals (retro orbital, IC in mice & rats, and ear vein in rabbits) and large animals (cephalic, saphenous, IC, etc.) Handle mice, rats, hamster, rabbits, guinea pigs, cats, dogs, swine, sheep, goats and primates. Performs multiple projects with the emphasis on small animal PK/TOX studies and manages all aspects of these studies. These would include the dosing, bleeding, processing of the samples taken and all data associated with these studies. Will work closely with the Study Directors as it pertains to the studies. Daily responsibilities will included but are not limited to clinical observation, body weights, food consumption, ECG?s, etc. Generates and maintains all paperwork associated with each project. Assist with limited animal care activities, such as feeding, water and bedding/cage changes as necessary. Preparation and review of data packages, utilized in the collection of study raw data. * * Maintain conformance with quality mission statement, goals and values. Adhere to compliance with ISO 17025 certification through the laboratories and operations. Ensuring Compliance with health and safety programs, radiation and other regulatory programs.** * * Assist management in special projects, as requested. * * -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 From under017 <@t> umn.edu Tue Apr 14 11:48:09 2009 From: under017 <@t> umn.edu (Anne Undersander) Date: Tue Apr 14 11:48:15 2009 Subject: [Histonet] F4/80 Message-ID: <000001c9bd20$cb8209f0$f66e5486@ad.ahc.umn.edu> Hi Histonet, We have a working IHC protocol for F4/80 on mouse tissues. However, when we try to switch this procedure to IF it doesn't work. Would anyone please have any suggestions as to why this won't work? The pretreatment that we use is trypsin at 37 degrees for 15 min. Does this enzyme inhibit the Cy5 which is the secondary fluorophore that we're using? Any advice/help is greatly appreciated! Thanks! Anne From Janet.Bonner <@t> FLHOSP.ORG Tue Apr 14 12:03:04 2009 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Apr 14 12:07:51 2009 Subject: [Histonet] 5 Reasons References: <5d9104a30904140852h583d8516h25f3f3e423176afd@mail.gmail.com> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F29BB@fhosxchmb006.ADVENTISTCORP.NET> How about the ability to diversify among several curricula : special staining, IHC, sectioning, special procedures (muscle, nerve biopsies), Electron Microscopy, Immunoflourescence....... ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Tue 4/14/2009 11:52 AM To: Histonet Subject: [Histonet] 5 Reasons Please help me out here.... I am suppose to post 5 Reasons to become a histotech in our lab. It is a competition for lab week. Each department has to post 5 reasons you should work in their department. I am looking for some great answers.... Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From cathy <@t> wasatchhisto.com Tue Apr 14 12:31:28 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Tue Apr 14 12:31:50 2009 Subject: [Histonet] Tissue Tek II- last one Message-ID: <7A74A595CDD64A6D8AE23AD28417AD58@shop1e2e996aa5> Almost have the lab boxed up but I have 1Tissue Tek II staining tray with 12 staining dishes along with 14 more other staining dishes left. Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 From micropathlabs <@t> yahoo.com Tue Apr 14 13:11:05 2009 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Tue Apr 14 13:11:09 2009 Subject: [Histonet] 5 Reasons In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47807F29BB@fhosxchmb006.ADVENTISTCORP.NET> References: <5d9104a30904140852h583d8516h25f3f3e423176afd@mail.gmail.com> <5F31F38C96781A4FBE3196EBC22D47807F29BB@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <334666.94140.qm@web57808.mail.re3.yahoo.com> First on your list should be: To help to provide quality care to patients ? Sheila Haas Laboratory Supervisor Micro Path Laboratories ? ________________________________ From: "Bonner, Janet" To: Cindy DuBois ; Histonet Sent: Tuesday, April 14, 2009 1:03:04 PM Subject: RE: [Histonet] 5 Reasons How about the ability to diversify among several curricula : special staining, IHC, sectioning, special procedures (muscle, nerve biopsies), Electron Microscopy, Immunoflourescence....... ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Tue 4/14/2009 11:52 AM To: Histonet Subject: [Histonet] 5 Reasons Please help me out here.... I am suppose to post 5 Reasons to become a histotech in our lab.? It is a competition for lab week.? Each department has to post 5 reasons you should work in their department. I am looking for some great answers.... Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure.? If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited.? If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cathy <@t> wasatchhisto.com Tue Apr 14 13:28:59 2009 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Tue Apr 14 13:29:21 2009 Subject: [Histonet] Tissue Tek II gone Message-ID: <41B05A53BD6F43118D3AD749393E4A6C@shop1e2e996aa5> Thanks for all the inquiries but the Tissue Tek II staining tray and all the extra dishes are gone. Cathy A. Mayton Wasatch Histo Consultants, Inc. From pbaldwin <@t> theadvisoryboardprogram.com Tue Apr 14 13:44:31 2009 From: pbaldwin <@t> theadvisoryboardprogram.com (Peter Baldwin) Date: Tue Apr 14 13:45:29 2009 Subject: [Histonet] Xylene substitute Message-ID: Formula 83, like xylene and many xylene substitutes, is categorized as flammable according to its MSDS and, thus, must be handled as a hazardous waste in accordance with EPA regulations, including monitoring its exposure, storage, and disposal. Peter Peter G. Baldwin Director of Sales, Marketing & Business Development pbaldwin@MicronEnvironmental.com Micron Environmental Industries, Inc. Green Chemistry for LifeSM www.MicronEnvironmental.com 1221 Cameron Street Alexandria, VA 22314 703-548-2776 703-548-7988/Fax From matthewtclose <@t> gmail.com Tue Apr 14 14:01:23 2009 From: matthewtclose <@t> gmail.com (Matthew Close) Date: Tue Apr 14 14:01:27 2009 Subject: [Histonet] re: decaling bone Message-ID: <6abc767b0904141201s403a0ffcp1865a0cc79d7f5c6@mail.gmail.com> I don't think the bone matrix will re-calcify with normal bone histo solutions. What exactly are you using to decalcify the tissue? Hardening can occur at many steps following decal. I stopped using a commercial decalcifying agent (which consisted of formic acid with chelating agents added) because my tissue samples would harden more rapidly after dehydration, clearing and infiltration (paraffin) and wouldn't infiltrate properly. Not sure if it was the chelating additives or not, but I went back to using Formic A solution with 5-10% formic depending on the type of tissue. Matt From Lori.Disher <@t> HCAhealthcare.com Tue Apr 14 14:12:40 2009 From: Lori.Disher <@t> HCAhealthcare.com (Disher Lori) Date: Tue Apr 14 14:12:48 2009 Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts Message-ID: <778DD853CF606049A37FC2059C8BA07A2ACBE5D75B@FWDCWPMSGCMS04.hca.corpad.net> Hello, I was wondering if anyone has some histo trivial-fun facts to share for Lab Week? I remember a supervisor told me long ago that she was told while in training, that if you took a hard boilded egg and sectioned it at 2 microns you would have enough sections to cover a football field. Has anyone ever heard that one before? Can anyone contribute any others? We are trying to come up with some games for lab week. Lori From Barry.R.Rittman <@t> uth.tmc.edu Tue Apr 14 14:24:09 2009 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Apr 14 14:24:15 2009 Subject: [Histonet] RE: Lab Week-Histology Trivial or Fun Facts In-Reply-To: <778DD853CF606049A37FC2059C8BA07A2ACBE5D75B@FWDCWPMSGCMS04.hca.corpad.net> References: <778DD853CF606049A37FC2059C8BA07A2ACBE5D75B@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: <75A0543E23D3A7458012D9E02EDBEC0002E8077A61@UTHCMS1.uthouston.edu> Lori Trivia but may not be fun. A fact re electron microscope. Glass knives have been used in electron microscopy since around the 1950s for preparation of thin sections. (The best glass was from antique windows. As glass is a viscous material that is constantly flowing, antique glass has less strain lines in it.) However this was not the first instance in which glass knives were used. I believe the first use was by Gutav Mann. In his book on histologic technique in 1902 he recommended the use of glass instead of steel knives in those instances when tissues were to be examined for iron, as he was afraid that steel knives used during cutting would contaminate the tissue. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Disher Lori Sent: Tuesday, April 14, 2009 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts Hello, I was wondering if anyone has some histo trivial-fun facts to share for Lab Week? I remember a supervisor told me long ago that she was told while in training, that if you took a hard boilded egg and sectioned it at 2 microns you would have enough sections to cover a football field. Has anyone ever heard that one before? Can anyone contribute any others? We are trying to come up with some games for lab week. Lori _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMcCormick <@t> schosp.org Tue Apr 14 14:33:21 2009 From: JMcCormick <@t> schosp.org (McCormick, James) Date: Tue Apr 14 14:33:27 2009 Subject: [Histonet] Embedding Stamp??? In-Reply-To: <7c363b8a0904140654i5b514858na8950f4051f01e46@mail.gmail.com> References: <7c363b8a0904140654i5b514858na8950f4051f01e46@mail.gmail.com> Message-ID: <0672286797B07E40AA3414F534B7CB80040A7C82E9@EXCHCCRMB.schosp.org> Histonet friends, Try using the "hex" head of a 3/8 inch diameter 1-2 1/2" long bolt. This works quite well and costs about 15 cents at the hardware store. J.B.McCormick, M.D. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yeonju Shim Sent: Tuesday, April 14, 2009 8:54 AM To: histonet; histonet-owner@lists.utsouthwestern.edu Subject: [Histonet] Embedding Stamp??? Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anne.lewin <@t> bms.com Tue Apr 14 14:39:38 2009 From: anne.lewin <@t> bms.com (Lewin, Anne) Date: Tue Apr 14 14:39:46 2009 Subject: [Histonet] RE: Lab Week-Histology Trivial or Fun Facts In-Reply-To: <778DD853CF606049A37FC2059C8BA07A2ACBE5D75B@FWDCWPMSGCMS04.hca.corpad.net> References: <778DD853CF606049A37FC2059C8BA07A2ACBE5D75B@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: <4895A1696F956D4CB56011A8C6131282010098E177@ushpwbmsmmp008.one.ads.bms.com> Histology world has some cute games and fun facts: http://www.histology-world.com/ >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Disher Lori >Sent: Tuesday, April 14, 2009 3:13 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts > >Hello, > I was wondering if anyone has some histo trivial-fun facts to share >for Lab Week? I remember a supervisor told me long ago that she was >told while in training, that if you took a hard boilded egg and >sectioned it at 2 microns you would have enough sections to cover a >football field. Has anyone ever heard that one before? Can anyone >contribute any others? We are trying to come up with some games for lab >week. >Lori > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stephanie.d.rivera <@t> gsk.com Tue Apr 14 15:01:04 2009 From: stephanie.d.rivera <@t> gsk.com (stephanie.d.rivera@gsk.com) Date: Tue Apr 14 15:01:23 2009 Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts In-Reply-To: <778DD853CF606049A37FC2059C8BA07A2ACBE5D75B@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: Hi All, No. I never heard the hard boiled egg sectioning fact. 1. Match the baby picture to the tech. 2. Gather information on if one weren't a histotech what would one do? Create a form and have the techs match names to the profession. It could be fun. I participated in another lab once and the techs were funny. If I weren't a histotech I'd travel on the speech circuit. That particular person talked all day, literally. OR you can come up with professions based on the techs personality and have everyone guess on the form. Who ever get the most correct gets $5.00 Wawa card or something. 3. Unscramble laboratory terms from all labs including clinical. 4.Double check this info, Who developed the diamond knife for electron microscopy AND improved the ultramicrotome Answer: Humberto Fernandez-Moran--- Venezuela Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 "Disher Lori" Sent by: histonet-bounces@lists.utsouthwestern.edu 14-Apr-2009 15:12 To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Lab Week-Histology Trivial or Fun Facts Hello, I was wondering if anyone has some histo trivial-fun facts to share for Lab Week? I remember a supervisor told me long ago that she was told while in training, that if you took a hard boilded egg and sectioned it at 2 microns you would have enough sections to cover a football field. Has anyone ever heard that one before? Can anyone contribute any others? We are trying to come up with some games for lab week. Lori _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Tue Apr 14 15:07:06 2009 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Tue Apr 14 15:07:17 2009 Subject: [Histonet] Embedding Stamp??? In-Reply-To: <0672286797B07E40AA3414F534B7CB80040A7C82E9@EXCHCCRMB.schosp.org> References: <7c363b8a0904140654i5b514858na8950f4051f01e46@mail.gmail.com> <0672286797B07E40AA3414F534B7CB80040A7C82E9@EXCHCCRMB.schosp.org> Message-ID: Sakura used to sell items called "Tissue Tampers" just for this purpose in different sizes. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Yeonju Shim >Sent: Tuesday, April 14, 2009 8:54 AM >To: histonet; histonet-owner@lists.utsouthwestern.edu >Subject: [Histonet] Embedding Stamp??? > >Hi, >I am trying to order a tool that I can flatten wavy tissues down at the >bottom of the mold for embedding. >It looks like a little metal square (kind of) stamp. >Do anyone know what it's called and where I can order? >Thank you, >Judy -- From rgrow <@t> bmnet.com Tue Apr 14 15:28:25 2009 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Tue Apr 14 15:28:30 2009 Subject: [Histonet] Job opening in East Tennessee Message-ID: Blount Memorial Hospital in Maryville, TN has a histology technician position open Monday thru Friday 7-3:30. We are located just minutes from the beautiful Smoky Mountains National Park and experience 4 wonderful seasons! All types of outdoor activities are possible. Maryville is host to the annual Foothills Fall Festival with top name entertainment and crafts, and just 30 minutes from Knoxville's culture events and entertainment, as well as UT football, basketball, etc. Applicants must meet the educational and training requirements necessary for certification by the American Society of Clinical Pathology as a Histology Technician or have experience equal to certification. General histology experience preferred. Must demonstrate competency and successfully complete the on-the-job orientation through the histology section of the laboratory. Performs all duties of a Histology Technician and other duties as assigned. Technically, it is listed as part time, but there will be 40 hrs/wk needed. Good benefits are offered. Anyone interested please visit our website at blountmemorial.org. to fill out an application and attach a resume. Or, you may just send me a resume. Thanks, Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. From TJJ <@t> stowers.org Tue Apr 14 15:29:12 2009 From: TJJ <@t> stowers.org (Johnson, Teri) Date: Tue Apr 14 15:29:54 2009 Subject: [Histonet] 2009 MO Society for Histotechnology Symposium Message-ID: Mark your calendar for this years MO Society for Histotechnology Symposium to be held at the beautiful Lake of the Ozarks, Port Arrowhead Resort on May 29-30, with a vendor reception and MSH Quarterly Business Meeting on Thursday evening, May 28. Our program is as follows: Thursday, May 28: 6:00 pm - 8:00 pm - Registration and Exhibit Hall Open 8:00 pm - Enjoy the Portside Lounge with friends or attend the MSH Quarterly Business Meeting Friday, May 29: 7:00 - 7:45 am - Registration 7:45 am - Welcome - Amanda Kelley, MSH President 8:00 - 9:30 - Brave New World: Introduction to Molecular Pathology, Part 1 - Dr. Thomas Haas, DO, FASCP 9:30 - 10:00 - Exhibit Hall Open 10:00 - 11:15 - Brave New World: Introduction to Molecular Pathology, Part 2 - Dr. Thomas Haas, DO, FASCP 11:30 - 12:30 - Green Histology - Laurence Patton BS, HT(ASCP) 12:30 - 1:15 - Lunch on your own 1:30 - 5:00 Workshop 1 - Muscle Biopsies: Gross Room to Reading Room - Konnie Zeitner, HT(ASCP)HTL SLS Workshop 2 - The Histology Workout: Getting Lean in the Lab - Christine "Charlie" Dorner, HT(ASCP)QIHC Workshop 3 - Methyl Methacrylate - Why and How? - Jack Ratliff, BA 6:00 - 8:00 Tropic Island Dinner Cruise (Cash Bar) Saturday, May 30 7:00 - 7:45 am - Registration 7:45 - Welcome - JP Rey, MSH Vice-President 8:00 - 8:45 - Mohs at a Glance, The Benefits of Moh's Surgery - Gina Rodriguez, HT(ASCP) 8:45 - 10:00 - Where We are and Where we have been with Tissue Processing, Part 1 - Christine "Charlie" Dorner, HT(ASCP)QIHC 10:00 - 10:30 - Exhibit Hall Open 10:30 - 12:00 - Where We are and Where we have been with Tissue Processing, Part 2 - Christine "Charlie" Dorner, HT(ASCP)QIHC 12:00 - 1:30 - MSH Awards lunch with Exhibitors 1:30 - 4:30 Workshop 4 - The Clinical, Technical, and Financial Benefits of Multi-antigen Immunostaining (MAIS) Procedures - Joseph D. Myers, MS, CT(ASCP) Workshop 5 - Tissue Identification for the Histotechnologist - Dr. Thomas Haas, DO, FASCP Workshop 6 - The What, When, Where, and How of Disaster Preparedness - Sylvia Jackson Casey, HT(ASCP), MA/MA All workshops are CEU approved by NSH. For complete information and a pdf copy of the brochure, visit www.missouri-histo.org or email Amanda Kelley kelleypath1@charter.net or JP Rey jp1000r@hotmail.com Hotel reservation deadline is April 27, 2009, and the toll free number is 1-800-532-3575. Let them know you're attending the MSH symposium for a reduced room rate. From rjbuesa <@t> yahoo.com Tue Apr 14 16:01:47 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 14 16:01:51 2009 Subject: [Histonet] decaling bones In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D8313C@EMAIL.archildrens.org> Message-ID: <940132.75988.qm@web65702.mail.ac4.yahoo.com> No, once it is decalcified, that's it! Ren? J. --- On Tue, 4/14/09, Horn, Hazel V wrote: From: Horn, Hazel V Subject: [Histonet] decaling bones To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 14, 2009, 12:04 PM We are having the hardest time decaling bones. Femurs and tibias. Is there such as thing as overdecaling and the bone becoming hard again? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kris.Caldwell <@t> leica-microsystems.com Tue Apr 14 16:07:55 2009 From: Kris.Caldwell <@t> leica-microsystems.com (Kris.Caldwell@leica-microsystems.com) Date: Tue Apr 14 16:08:01 2009 Subject: [Histonet] Sales Account Executive- Chicagoland Message-ID: This person will need to be based in Northern Illinois or Southern Wisconsin. Leica Microsystems is a leading global designer and producer of innovative high-tech precision optics systems for the analysis of microstructures. It is one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries, Leica Microsystems is a leading global designer and producer of innovative high-tech precision optics systems for the analysis of microstructures. It is one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. To achieve Leica sales and profitability goals within an assigned territory through the implementation of aggressive, direct end-user selling techniques.?Promote Leica as the Histology market leader in quality and innovation. - Achieve monthly, quarterly, annual and strategic product mix sales goals for the territory. - Plan and schedules face-to-face account calls to current and potential end-users of Leica products. -?Identify and develop key accounts in the territory. -?Manage assigned national accounts within territory requiring corporate coordination to enable closure and compliance of contracts. -?Install/set-up instrumentation in customer laboratories.? Perform demonstrations.? Maintain demonstration equipment in a clean and operational manner. Train customers on the use of instrumentation. Prepare monthly territory status reports to Regional Sales Manager (including, but not limited to, Target Account Lists, Won/Lost accounts, Forecasts, expense reports and travel calendars). Maintain current knowledge of competition and market through study of competitive marketing information, competitive literature, and field surveillance of competition. Requirements -?? BA/BS in Life Sciences or equivalent -?? 1-3 years sales experience in capital equipment -?? Demonstrated success in selling -?? Experience selling Microtomes, cryostats, or autostainers -?? Understanding of Histology marketplace or a related discipline -?? 1-3 years Histology laboratory experience in clinical, research or industrial setting desirable but not required - Histotechnologist background is a plus -?? Good selling, negotiating, closing and account management skills -?? Self motivated -?? Goal oriented, results driven -?? Good interpersonal skills -?? Ability to comprehend scientific applications/markets -?? Good time management, good organizational skills -?? Works independently but able to interact as a team member -?? Good communications skills Proficient in computer skills (Excel, Word, PowerPoint, Lotus Notes, SAP) Send your resume to- kris.caldwell@leica-microsystems.com Leica offers competitive salary, benefits including medical, dental, vision, prescription, long-term care, life insurance, STD, LTD, and 401 (k). Please, no solicitations from 3rd Party Vendors. Thank you. Kris Caldwell Human Resources Recruiter Leica Microsystems, Inc. 2345 Waukegan Road Bannockburn, IL 60015 www.leica-microsystems.com Kris.Caldwell@Leica-Microsystems.com 847-405-5432 - phone 847-236-3035 - fax 847-323-6169- cellular ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From AnthonyH <@t> chw.edu.au Tue Apr 14 16:13:44 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 14 16:13:55 2009 Subject: [Histonet] Embedding Stamp??? In-Reply-To: <7c363b8a0904140654i5b514858na8950f4051f01e46@mail.gmail.com> Message-ID: Try Thermo Shandon or any of the histo equipment suppliers. If they sell embedding centres then they are bound to have the "tampers" Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yeonju Shim Sent: Tuesday, 14 April 2009 11:54 PM To: histonet; histonet-owner@lists.utsouthwestern.edu Subject: [Histonet] Embedding Stamp??? Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Tue Apr 14 16:16:49 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 14 16:16:56 2009 Subject: [Histonet] TBS vs PBS In-Reply-To: <3809C163DC1DA54AA534B3C7794D07B63298E4C546@ENHBGMBX01.PA.LCL> Message-ID: I am not sure of the effect of buffer type on the protease but it might be possible that the proteinase K is the problem. My experience is that they can vary between lots. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Wednesday, 15 April 2009 12:41 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] TBS vs PBS Hello all, Would proteinase K diluted in PBS be any different then PK diluted in Tris buffer when used for a fluorescent antibody test? I'm having trouble duplicating a published testing method and the only difference is PK diluted in TBS instead of PBS. Thanks in advance for all the wonderful information from all. Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rcharles@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From HornHV <@t> archildrens.org Tue Apr 14 16:17:19 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Apr 14 16:17:25 2009 Subject: [Histonet] decaling bones In-Reply-To: <940132.75988.qm@web65702.mail.ac4.yahoo.com> References: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D8313C@EMAIL.archildrens.org> <940132.75988.qm@web65702.mail.ac4.yahoo.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83142@EMAIL.archildrens.org> The bone appears soft and easy to cut with a razor blade but after processing the tissue is as hard as brick. Any suggestions? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Tuesday, April 14, 2009 4:02 PM To: histonet@lists.utsouthwestern.edu; Horn, Hazel V Subject: Re: [Histonet] decaling bones No, once it is decalcified, that's it! Ren? J. --- On Tue, 4/14/09, Horn, Hazel V wrote: From: Horn, Hazel V Subject: [Histonet] decaling bones To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 14, 2009, 12:04 PM We are having the hardest time decaling bones. Femurs and tibias. Is there such as thing as overdecaling and the bone becoming hard again? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From AnthonyH <@t> chw.edu.au Tue Apr 14 16:19:04 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Apr 14 16:19:10 2009 Subject: [Histonet] 5 Reasons In-Reply-To: <5d9104a30904140852h583d8516h25f3f3e423176afd@mail.gmail.com> Message-ID: Cindy, A much respected and knowledgeable Histotech (who is now retired) said: "Histotechnology - you either hate it or love it with a passion!" Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Wednesday, 15 April 2009 1:52 AM To: Histonet Subject: [Histonet] 5 Reasons Please help me out here.... I am suppose to post 5 Reasons to become a histotech in our lab. It is a competition for lab week. Each department has to post 5 reasons you should work in their department. I am looking for some great answers.... Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Barry.R.Rittman <@t> uth.tmc.edu Tue Apr 14 16:36:12 2009 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Apr 14 16:36:16 2009 Subject: [Histonet] decaling bones In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83142@EMAIL.archildrens.org> References: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D8313C@EMAIL.archildrens.org> <940132.75988.qm@web65702.mail.ac4.yahoo.com> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83142@EMAIL.archildrens.org> Message-ID: <75A0543E23D3A7458012D9E02EDBEC0002E8077A9B@UTHCMS1.uthouston.edu> Hazel Processing using alcohol then xylene? If so then suspect too long in alcohol or using xylene is your major problem with paraffin wax a close second. Can use chloroform as an intermediary agent and bone will not harden as much. If you must use xylene, try to soak in a mixture of xylene:paraffin wax (1:1) at room temperature for at least a few hours. This allows some wax to penetrate into the tissue and cuts down time in paraffin wax. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, April 14, 2009 4:17 PM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] decaling bones The bone appears soft and easy to cut with a razor blade but after processing the tissue is as hard as brick. Any suggestions? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Tuesday, April 14, 2009 4:02 PM To: histonet@lists.utsouthwestern.edu; Horn, Hazel V Subject: Re: [Histonet] decaling bones No, once it is decalcified, that's it! Ren? J. --- On Tue, 4/14/09, Horn, Hazel V wrote: From: Horn, Hazel V Subject: [Histonet] decaling bones To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 14, 2009, 12:04 PM We are having the hardest time decaling bones. Femurs and tibias. Is there such as thing as overdecaling and the bone becoming hard again? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Tue Apr 14 16:55:54 2009 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Apr 14 16:56:00 2009 Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts In-Reply-To: References: <778DD853CF606049A37FC2059C8BA07A2ACBE5D75B@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: <75A0543E23D3A7458012D9E02EDBEC0002E8077AA0@UTHCMS1.uthouston.edu> You are correct about the diamond knife introduced by Humberto Fernandez-Moran in 1953 (Exp. Cell Res. 5. 255. 1953). He was a very innovative electron microscopist, introducing single filament etc. Can see his biography on Google. The use of glass knives in electron microscopy was introduced by H. Latta and J. F. Hartmann, "The use of a Glass Edge in Thin Sectioning for Electron Microscopy". Proc. Soc. Exptl. Biol. Med. 74. 436-439. 1950 Referenced in "Introduction to Electron Microscopy" by Cecil E. Hall. McGraw Hill Book Co., p357. 1953. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of stephanie.d.rivera@gsk.com Sent: Tuesday, April 14, 2009 3:01 PM To: Disher Lori Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Lab Week-Histology Trivial or Fun Facts Hi All, No. I never heard the hard boiled egg sectioning fact. 1. Match the baby picture to the tech. 2. Gather information on if one weren't a histotech what would one do? Create a form and have the techs match names to the profession. It could be fun. I participated in another lab once and the techs were funny. If I weren't a histotech I'd travel on the speech circuit. That particular person talked all day, literally. OR you can come up with professions based on the techs personality and have everyone guess on the form. Who ever get the most correct gets $5.00 Wawa card or something. 3. Unscramble laboratory terms from all labs including clinical. 4.Double check this info, Who developed the diamond knife for electron microscopy AND improved the ultramicrotome Answer: Humberto Fernandez-Moran--- Venezuela Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 "Disher Lori" Sent by: histonet-bounces@lists.utsouthwestern.edu 14-Apr-2009 15:12 To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Lab Week-Histology Trivial or Fun Facts Hello, I was wondering if anyone has some histo trivial-fun facts to share for Lab Week? I remember a supervisor told me long ago that she was told while in training, that if you took a hard boilded egg and sectioned it at 2 microns you would have enough sections to cover a football field. Has anyone ever heard that one before? Can anyone contribute any others? We are trying to come up with some games for lab week. Lori _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Tue Apr 14 17:54:23 2009 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Apr 14 17:54:49 2009 Subject: [Histonet] Freezing rodent brains for Mass Spec In-Reply-To: <75A0543E23D3A7458012D9E02EDBEC0002E8077AA0@UTHCMS1.uthouston.edu> Message-ID: I have a colleague who requires snap frozen rodent brains to perform Mass Spec on 10micron frozen sections mounted on plates. Because OCT interferes with mass spec, they are currently using ethanol to slow the freezing process to prevent cracking of the brain in liquid nitrogen. The alcohol, however, causes some detriment to the surface of the brains - in science-speak "they get mushy". Does anyone have any suggestions for snap freezing brains without any substrate while preventing cracking? Jackie O' From integrated.histo <@t> gmail.com Tue Apr 14 18:53:02 2009 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Tue Apr 14 18:53:06 2009 Subject: [Histonet] Histo Trivia Message-ID: <5d9104a30904141653m35bac661s9c722ada3e52f3e3@mail.gmail.com> The NSH journal that just came had an article on the history of Histology. I am only halfway through the article but their were some interesting facts that you could probably use. I also want to thank all of you who helped me put together 5 reasons to work in histology. I am paring the list down to 5 and will post them here probably tomorrow. Cindy From Tony_Reilly <@t> health.qld.gov.au Tue Apr 14 19:30:42 2009 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Tue Apr 14 19:31:58 2009 Subject: [Histonet] (no subject) In-Reply-To: <000601c9bd19$76a1ff50$3394640a@yrmc.org> References: <000601c9bd19$76a1ff50$3394640a@yrmc.org> Message-ID: <49E5B751.471C.0039.0@health.qld.gov.au> I have used DAKO Target Retrieval in the past and it is definitely possible to re-use the solution. The important thing to do is to check the pH each day before use as this is the best indicator that the solution needs to be changed. It has bee an while since I used it but from my shaky memory you should get at least a week from the solution. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> "jeff" 15/04/2009 1:55 am >>> Is anyone using their target retrieval for dako more than 1 time? Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From kwuny <@t> email.cs.nsw.gov.au Tue Apr 14 22:06:00 2009 From: kwuny <@t> email.cs.nsw.gov.au (Young Kwun) Date: Tue Apr 14 22:06:11 2009 Subject: [Histonet] Fli-1 antibody - Please help Message-ID: <002201c9bd77$1ace85f0$c680310a@cs.nsw.gov.au> Dear Histonetters, I was wondering whether anyone had success in Fli-1 immunohistochemistry on formalin-fixed, paraffin embedded human sections. I tried two polyclonal, commercial antibodies from abcam (ab15289) and Santa Cruz Biotech's Fli-1 (C-19, sc-356) without any positive stainings. Both antibodies were recommended for application on paraffin sections. Abcam's application notes even mentioned that perform enzymatic antigen retrieval BEFORE commencing with HIER procedure. The procedure still did not give any stains and the morphology was unacceptable. I tried with/without HIER with different buffers as well. I used primary dilutions 1:50 - 1:200 as recommended and I am using Leica's BondMax Autostainer. Any comments would be appreciated. Young Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au From kwuny <@t> email.cs.nsw.gov.au Tue Apr 14 22:29:24 2009 From: kwuny <@t> email.cs.nsw.gov.au (Young Kwun) Date: Tue Apr 14 22:29:30 2009 Subject: FW: [Histonet] (no subject) Message-ID: <002a01c9bd7a$5f7a9a60$c680310a@cs.nsw.gov.au> I also used Dako's Target Retrieval buffer in the past and it was also possible to dilute further up to 1:20 or 1:40 (instead of 1:10) with good results. Young -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony Reilly Sent: Wednesday, 15 April 2009 10:31 AM To: histonet@lists.utsouthwestern.edu; jeff Subject: Re: [Histonet] (no subject) I have used DAKO Target Retrieval in the past and it is definitely possible to re-use the solution. The important thing to do is to check the pH each day before use as this is the best indicator that the solution needs to be changed. It has bee an while since I used it but from my shaky memory you should get at least a week from the solution. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> "jeff" 15/04/2009 1:55 am >>> Is anyone using their target retrieval for dako more than 1 time? Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************************** ****** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____________________________________________________________________ This email has been scanned for the Sydney South West Area Health Service by the MessageLabs Email Security System. SSWAHS regularly monitors emails and attachments to ensure compliance with the NSW Government's Electronic Messaging Policy. "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From koellingr <@t> comcast.net Tue Apr 14 23:01:55 2009 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue Apr 14 23:01:57 2009 Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts LONG OFF TOPIC In-Reply-To: <778DD853CF606049A37FC2059C8BA07A2ACBE5D75B@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: <882626479.1023781239768115064.JavaMail.root@sz0001a.emeryville.ca.mail.comcast.net> Hi in grad school taking microanatomy and pathology classes, 2 that I heard are this:? The surface area of all the alveoli in the lungs of an adult is between 40-70 square meters. That seems reasonable in having a 40-70 square meter surface (where all gas exchange takes place) represent all the gas exchange in lungs. Have seen that figure numerous times so while can't test it, can believe it.? The other one that I also can't test and is hard to believe is that the sum total length of all vessels (large small, artery vein down to every single capillary) in one adult measures about 100,000 kilometers (62,000 miles). Again there are many disparate medical and anatomical references so either all right or all wrong. The 2 micron sectioned egg I don't believe. (1) There are 25,400 microns in an inch. A 2 inch long egg is about 50,000 microns long. At 2 microns per section thats about 25,000 egg sections.? Even is each section is 2 square inches (that's generous since each end isn't close to 2 squre inches in area), thats 100,000 square inches. At 1,296 square inches per square yard, that's about 40 square yards which is far short of a football field (100 yards x 53 yards). (2) If you calculate the volume of a "solid rectangle" covering a football feild that is 100 yards x 53 yards x 2 microns and of course converting all to yards or microns, the answer is a specific volume.? If you take the volume of an ellipsoid which is four thirds times pi times a times b times c with a, b and c being the lenggth of the 3 axis of the ellipsoid, and using approximate measurements for the egg, I come up with far , far less volume in egg than in the "rectangular solid" covering football field. (3) This is a classical calculus definte integral washer problem. Whether this egg as an ellipsoid is scalene, oblate or prolate, integrating volume over the limits of integration gives me much, much less volume than is needed to cover a football field 2 microns thick.? Have tried all 3 methods and converting everything to? microns or yards using scientific notation. So 6 calculations.? Everytime I come up somewhere close to the area of 2 micron slices covering approximately 1/100 of the football field. Unless my math is all wrong, or this is a humongous, enormous?ostrich and not chicken egg. Ray Raymond Koelling PhenoPath Labs Seattle, WA ----- Original Message ----- From: "Disher Lori" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 14, 2009 12:12:40 PM GMT -08:00 US/Canada Pacific Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts Hello, ??I was wondering if anyone has some histo trivial-fun facts to share for Lab Week? ?I remember a supervisor told me long ago that she was told while in training, that if you took a hard boilded egg and sectioned it at 2 microns you would have enough sections to cover a football field. ?Has anyone ever heard that one before? ?Can anyone contribute any others? ?We are trying to come up with some games for lab week. Lori _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tony_Reilly <@t> health.qld.gov.au Wed Apr 15 00:54:39 2009 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Wed Apr 15 00:55:30 2009 Subject: FW: [Histonet] (no subject) In-Reply-To: <002a01c9bd7a$5f7a9a60$c680310a@cs.nsw.gov.au> References: <002a01c9bd7a$5f7a9a60$c680310a@cs.nsw.gov.au> Message-ID: <49E6033E.471C.0039.0@health.qld.gov.au> As per Young's response, I have never diluted the solution further but found that if there was some drop in the level of the solution due to evaporation it could be topped up with deionised water as long as the pH was not changed. This was on the advice of the DAKO rep at the time. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> "Young Kwun" 15/04/2009 1:29 pm >>> I also used Dako's Target Retrieval buffer in the past and it was also possible to dilute further up to 1:20 or 1:40 (instead of 1:10) with good results. Young -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony Reilly Sent: Wednesday, 15 April 2009 10:31 AM To: histonet@lists.utsouthwestern.edu; jeff Subject: Re: [Histonet] (no subject) I have used DAKO Target Retrieval in the past and it is definitely possible to re-use the solution. The important thing to do is to check the pH each day before use as this is the best indicator that the solution needs to be changed. It has bee an while since I used it but from my shaky memory you should get at least a week from the solution. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> "jeff" 15/04/2009 1:55 am >>> Is anyone using their target retrieval for dako more than 1 time? Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************************** ****** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____________________________________________________________________ This email has been scanned for the Sydney South West Area Health Service by the MessageLabs Email Security System. SSWAHS regularly monitors emails and attachments to ensure compliance with the NSW Government's Electronic Messaging Policy. "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Wed Apr 15 02:54:55 2009 From: tifei <@t> foxmail.com (TF) Date: Wed Apr 15 02:55:18 2009 Subject: [Histonet] BrdU + TUNEL, which one first Message-ID: <200904151554499709006@foxmail.com> Hi all, in many IHC co-labeling with BrdU, we perform the other antigen first. How about TUNEL? Do BrdU antigen retrieval (HCL or citrate buffer) affect TUNEL staining? Thanks very much. 2009-04-15 TF From ree3 <@t> leicester.ac.uk Wed Apr 15 03:46:11 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed Apr 15 03:46:25 2009 Subject: [Histonet] Leica TP 1020 processor Message-ID: <7722595275A4DD4FA225B92CDBF174A17457783CCF@EXC-MBX3.cfs.le.ac.uk> Does anybody know anything good or bad even about the above tissue processor, for example can it be used on the open bench?, without any external extraction. Many thanks Richard Edwards Leicester University...U.K. From nyilmaz <@t> mersin.edu.tr Wed Apr 15 03:59:37 2009 From: nyilmaz <@t> mersin.edu.tr (Nejat Yilmaz) Date: Wed Apr 15 04:03:49 2009 Subject: [Histonet] Antibody for ZP Proteins Message-ID: <000601c9bda8$818210b0$2101a8c0@nejat1> Dear Colleagues, We're planning to study ZP1, ZP2 and ZP3 protein immunohistochemistry on mice ovary. We couldn't find commercially available antibodies for these proteins suitable for IHC. Does anybody know sources for buying this items? Thanks in advance... Dr. Necat Yilmaz MD, PhD University of Mersin From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Apr 15 06:00:23 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Apr 15 06:00:28 2009 Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts LONG OFF TOPIC Message-ID: <86ADE4EB583CE64799A9924684A0FBBF068342B4@wahtntex2.waht.swest.nhs.uk> I can only say Pathology needs more kids like you. I've no idea what your saying but it looks very interesting... Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: 15 April 2009 05:02 To: Disher Lori Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Lab Week-Histology Trivial or Fun Facts LONG OFF TOPIC Hi in grad school taking microanatomy and pathology classes, 2 that I heard are this:? The surface area of all the alveoli in the lungs of an adult is between 40-70 square meters. That seems reasonable in having a 40-70 square meter surface (where all gas exchange takes place) represent all the gas exchange in lungs. Have seen that figure numerous times so while can't test it, can believe it.? The other one that I also can't test and is hard to believe is that the sum total length of all vessels (large small, artery vein down to every single capillary) in one adult measures about 100,000 kilometers (62,000 miles). Again there are many disparate medical and anatomical references so either all right or all wrong. The 2 micron sectioned egg I don't believe. (1) There are 25,400 microns in an inch. A 2 inch long egg is about 50,000 microns long. At 2 microns per section thats about 25,000 egg sections.? Even is each section is 2 square inches (that's generous since each end isn't close to 2 squre inches in area), thats 100,000 square inches. At 1,296 square inches per square yard, that's about 40 square yards which is far short of a football field (100 yards x 53 yards). (2) If you calculate the volume of a "solid rectangle" covering a football feild that is 100 yards x 53 yards x 2 microns and of course converting all to yards or microns, the answer is a specific volume.? If you take the volume of an ellipsoid which is four thirds times pi times a times b times c with a, b and c being the lenggth of the 3 axis of the ellipsoid, and using approximate measurements for the egg, I come up with far , far less volume in egg than in the "rectangular solid" covering football field. (3) This is a classical calculus definte integral washer problem. Whether this egg as an ellipsoid is scalene, oblate or prolate, integrating volume over the limits of integration gives me much, much less volume than is needed to cover a football field 2 microns thick.? Have tried all 3 methods and converting everything to? microns or yards using scientific notation. So 6 calculations.? Everytime I come up somewhere close to the area of 2 micron slices covering approximately 1/100 of the football field. Unless my math is all wrong, or this is a humongous, enormous?ostrich and not chicken egg. Ray Raymond Koelling PhenoPath Labs Seattle, WA ----- Original Message ----- From: "Disher Lori" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 14, 2009 12:12:40 PM GMT -08:00 US/Canada Pacific Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts Hello, ??I was wondering if anyone has some histo trivial-fun facts to share for Lab Week? ?I remember a supervisor told me long ago that she was told while in training, that if you took a hard boilded egg and sectioned it at 2 microns you would have enough sections to cover a football field. ?Has anyone ever heard that one before? ?Can anyone contribute any others? ?We are trying to come up with some games for lab week. Lori _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Apr 15 06:13:28 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Apr 15 06:13:35 2009 Subject: [Histonet] 5 Reasons Message-ID: <86ADE4EB583CE64799A9924684A0FBBF068342BA@wahtntex2.waht.swest.nhs.uk> The Technical Staff in Pathology seem to fall into two types; those that manage machines and computers and try to optimise their outputs. The others use manual skills to create something, be it a slide, smear or result from an agar plate (although I concede Micro is becoming automated). I guess you choose the discipline that reflects your skills and I guess the skills of a HistTech are manual dexterity, good hand eye co-ordination and the ability to follow a procedure without deviation. We don't have the machines, by and large, to automate our procedures (except for H&E, Immunocytochemistry and many of the routine stains). I guess one of the 5 is how you personally are 'wired'; are you a techy or are you a craftsman. I don't believe that any of those genre to be subservient to the other, just different skills. You could turn a Microbiologist into a Histotech but can you turn a Chemist or a Haematologist? As a Histotech that became a Cytologist (a morphologist) and then a Manager, I'm interested in the skills that make us good (or not) at what we do; I for one am still looking (g). Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: 14 April 2009 16:52 To: Histonet Subject: [Histonet] 5 Reasons Please help me out here.... I am suppose to post 5 Reasons to become a histotech in our lab. It is a competition for lab week. Each department has to post 5 reasons you should work in their department. I am looking for some great answers.... Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Wed Apr 15 06:19:10 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Wed Apr 15 06:19:18 2009 Subject: [Histonet] 5 Reasons In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF068342BA@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF068342BA@wahtntex2.waht.swest.nhs.uk> Message-ID: <001d01c9bdbc$0027f610$0077e230$@net> You will create a work of art everyday that may save or extend a life. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Wednesday, April 15, 2009 7:13 AM To: Cindy DuBois; Histonet Subject: RE: [Histonet] 5 Reasons The Technical Staff in Pathology seem to fall into two types; those that manage machines and computers and try to optimise their outputs. The others use manual skills to create something, be it a slide, smear or result from an agar plate (although I concede Micro is becoming automated). I guess you choose the discipline that reflects your skills and I guess the skills of a HistTech are manual dexterity, good hand eye co-ordination and the ability to follow a procedure without deviation. We don't have the machines, by and large, to automate our procedures (except for H&E, Immunocytochemistry and many of the routine stains). I guess one of the 5 is how you personally are 'wired'; are you a techy or are you a craftsman. I don't believe that any of those genre to be subservient to the other, just different skills. You could turn a Microbiologist into a Histotech but can you turn a Chemist or a Haematologist? As a Histotech that became a Cytologist (a morphologist) and then a Manager, I'm interested in the skills that make us good (or not) at what we do; I for one am still looking (g). Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: 14 April 2009 16:52 To: Histonet Subject: [Histonet] 5 Reasons Please help me out here.... I am suppose to post 5 Reasons to become a histotech in our lab. It is a competition for lab week. Each department has to post 5 reasons you should work in their department. I am looking for some great answers.... Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From resendes.ana <@t> gmail.com Wed Apr 15 06:55:07 2009 From: resendes.ana <@t> gmail.com (Ana Resendes) Date: Wed Apr 15 06:55:13 2009 Subject: [Histonet] BrdU + TUNEL, which one first In-Reply-To: <200904151554499709006@foxmail.com> References: <200904151554499709006@foxmail.com> Message-ID: I used proteinase K....for Tunel antigen retrieval... 2009/4/15 TF > Hi all, in many IHC co-labeling with BrdU, we perform the other antigen > first. > How about TUNEL? > Do BrdU antigen retrieval (HCL or citrate buffer) affect TUNEL staining? > Thanks very much. > > 2009-04-15 > > > > TF > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Ana Resendes DVM, MSc, PhD, Veterinary Pathologist Postdoctoral DRCT-fellow research scientist Centro de Investiga??o de Recursos Naturais (CIRN) Laborat?rio de Microscopia e Histologia Departamento de Biologia, Universidade dos A?ores. Rua da M?e de Deus, 58 - Apartado 1422 P - 9501-801 Ponta Delgada (A?ores) Portugal Tel. (+351) 296 650 111 Fax (+351) 296 650 100 http://www.uac.pt/~pherg/ From putnamj <@t> ggclinic.com Wed Apr 15 07:55:46 2009 From: putnamj <@t> ggclinic.com (Putnam, Jodi) Date: Wed Apr 15 07:55:56 2009 Subject: [Histonet] Liver (control tissue for PAS/D) Message-ID: Hi again. I was wondering if anyone knew of a good source for liver tissue for PAS/D control blocks. I am currently having to buy them and it is ~42.00 for 10 slides. I am hoping to find a better price than that as my doctor orders a fair amount of these. I don't have access to autopsy tissue and when I did there was a problem with autolysis. I work in a derm lab so I am limited. I have asked several of my former employers and so far no luck. Any help acquiring the tissue or just telling me about a better price with a different vendor would be great. I will be totally out of tissue within ~2 weeks (if I am lucky to have it last that long). Thanks and everyone have a great day. Thanks to everyone for info for manuals. I am so glad I signed up for the histonet. Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From putnamj <@t> ggclinic.com Wed Apr 15 08:05:20 2009 From: putnamj <@t> ggclinic.com (Putnam, Jodi) Date: Wed Apr 15 08:05:30 2009 Subject: [Histonet] tissue control bank NSH Message-ID: Hi. I sent out a message before I thought to check with the NSH. I am a member and went to the site but am not finding the info on the tissue control bank. I tried to call but they are not open for another hour and I know that I will forget to call back then and be lost in the work flow for the day. Does anyone know how to access that on their site? I just need a little help finding it if it is on there. Thanks. Sorry if it's obvious on the site and I missed it. I haven't had any caffeine yet. Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Apr 15 08:31:37 2009 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Apr 15 08:31:42 2009 Subject: [Histonet] Liver (control tissue for PAS/D) Message-ID: <86ADE4EB583CE64799A9924684A0FBBF068342F5@wahtntex2.waht.swest.nhs.uk> I know this might be a stupid reply but as it's just for PAS/D can't you use animal liver? Why not pig's liver? Go to an slaughter house and get fresh pig's liver and bingo (I assume pig glycogen is the same as our's?)....... You could fry the residual with onions, very nice. Kemlo Rogerson e-mail kemlorogerson@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Putnam, Jodi Sent: 15 April 2009 13:56 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Liver (control tissue for PAS/D) Hi again. I was wondering if anyone knew of a good source for liver tissue for PAS/D control blocks. I am currently having to buy them and it is ~42.00 for 10 slides. I am hoping to find a better price than that as my doctor orders a fair amount of these. I don't have access to autopsy tissue and when I did there was a problem with autolysis. I work in a derm lab so I am limited. I have asked several of my former employers and so far no luck. Any help acquiring the tissue or just telling me about a better price with a different vendor would be great. I will be totally out of tissue within ~2 weeks (if I am lucky to have it last that long). Thanks and everyone have a great day. Thanks to everyone for info for manuals. I am so glad I signed up for the histonet. Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Wed Apr 15 08:35:45 2009 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Apr 15 08:35:49 2009 Subject: [Histonet] tissue control bank NSH In-Reply-To: References: Message-ID: Jodi, Go to NSH.org site. The link at the bottom takes you to this page. It can be found under About NSH/Board/Committees/click on QC committee. http://www.e-guana.net/organizations.php3?action=printContentItem&orgid=111&typeID=1162&itemID=17799William DeSalvo B.S. HTL(ASCP) Chair Person Quality Control Committee wdesalvo.cac@hotmail.com > Date: Wed, 15 Apr 2009 08:05:20 -0500 > From: putnamj@ggclinic.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] tissue control bank NSH > > Hi. I sent out a message before I thought to check with the NSH. I am a member and went to the site but am not finding the info on the tissue control bank. I tried to call but they are not open for another hour and I know that I will forget to call back then and be lost in the work flow for the day. Does anyone know how to access that on their site? I just need a little help finding it if it is on there. > Thanks. Sorry if it's obvious on the site and I missed it. I haven't had any caffeine yet. > > > Jodi Putnam (HT,ASCP) > Graves Gilbert Clinic > Pathology Department > 201 Park Street > Bowling Green, KY 42102 > (270) 393-2728 (voicemail) > (270) 393-2795 > Fax : (270) 393-2736 > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Graves-Gilbert Clinic. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Rediscover Hotmail?: Now available on your iPhone or BlackBerry http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Mobile1_042009 From b-frederick <@t> northwestern.edu Wed Apr 15 08:43:49 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Apr 15 08:44:02 2009 Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts In-Reply-To: Message-ID: <3F96010512CD447690CAF433C955D7C0@lurie.northwestern.edu> All, We actually brought in pictures of our parents one year for lab week. Another good one I saw was that someone snuck around and took pictures of people's feet- it was wild. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of stephanie.d.rivera@gsk.com Sent: Tuesday, April 14, 2009 3:01 PM To: Disher Lori Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Lab Week-Histology Trivial or Fun Facts Hi All, No. I never heard the hard boiled egg sectioning fact. 1. Match the baby picture to the tech. 2. Gather information on if one weren't a histotech what would one do? Create a form and have the techs match names to the profession. It could be fun. I participated in another lab once and the techs were funny. If I weren't a histotech I'd travel on the speech circuit. That particular person talked all day, literally. OR you can come up with professions based on the techs personality and have everyone guess on the form. Who ever get the most correct gets $5.00 Wawa card or something. 3. Unscramble laboratory terms from all labs including clinical. 4.Double check this info, Who developed the diamond knife for electron microscopy AND improved the ultramicrotome Answer: Humberto Fernandez-Moran--- Venezuela Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 "Disher Lori" Sent by: histonet-bounces@lists.utsouthwestern.edu 14-Apr-2009 15:12 To "histonet@lists.utsouthwestern.edu" cc Subject [Histonet] Lab Week-Histology Trivial or Fun Facts Hello, I was wondering if anyone has some histo trivial-fun facts to share for Lab Week? I remember a supervisor told me long ago that she was told while in training, that if you took a hard boilded egg and sectioned it at 2 microns you would have enough sections to cover a football field. Has anyone ever heard that one before? Can anyone contribute any others? We are trying to come up with some games for lab week. Lori _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Apr 15 08:45:29 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 15 08:45:36 2009 Subject: [Histonet] (no subject) In-Reply-To: <000601c9bd19$76a1ff50$3394640a@yrmc.org> Message-ID: <757892.38406.qm@web65705.mail.ac4.yahoo.com> Some people do, others do not. I never used it more than once and, since they are quite expensive, that is why I prepared my own. Ren? J. --- On Tue, 4/14/09, jeff wrote: From: jeff Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 14, 2009, 11:55 AM Is anyone using their target retrieval for dako more than 1 time? Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From integrated.histo <@t> gmail.com Wed Apr 15 08:53:20 2009 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Wed Apr 15 08:53:27 2009 Subject: [Histonet] 5 Reasons - The list Message-ID: <5d9104a30904150653u165d35f2k47cba9d3d433e294@mail.gmail.com> Thanks to so many of you I have come up with the following list: 1. You get to dig into peoples brains like no psychologist can. 2. Job security - no one else wants to work in the "human parts department". 3. It is never boring - "How did the patient get THAT in THERE?" 4. You get to do arts and crafts (Wax & Stains). 5. Most important - Providing quality care to patients. Cind Dubois Integrated Pathology Stockton, CA From mtitford <@t> aol.com Wed Apr 15 08:57:27 2009 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Apr 15 08:57:48 2009 Subject: [Histonet] More Lab Week trivia Message-ID: <8CB8BE93F108EE7-8A8-223D@webmail-dx16.sysops.aol.com> One year, everyone brought in photographs of their pets. There was a competition to guess which pets belonged to which lab employees. They say people look like their pets.... Mike Titford Pathology USA Mobile AL From jfray80 <@t> hotmail.com Wed Apr 15 09:35:20 2009 From: jfray80 <@t> hotmail.com (JOSEPH FRAZEE) Date: Wed Apr 15 09:35:24 2009 Subject: [Histonet] Shur-Wave Processor Message-ID: I would like some input on the Shur-Wave microwave system. I need to know pro's and con's. and is Isopropol alc. used on all processing. Thanks Histojoe _________________________________________________________________ Rediscover Hotmail?: Get quick friend updates right in your inbox. http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Updates1_042009 From ian.montgomery <@t> bio.gla.ac.uk Wed Apr 15 09:49:28 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Apr 15 09:51:14 2009 Subject: FW: [Histonet] decaling bones Message-ID: <1117E0CD8C214EECB4B900A446E0354F@IBLS.GLA.AC.UK> Hazel, Asked the boys here in Anatomy about your question and according to them if you're using a propriety decal agent you have to be careful as the bones do get harder if they are left in the solution. The calcium comes out but the bones are really tough to cut. Interestingly, it's not such a problem if using EDTA or acid, just the propriety agents. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: 14 April 2009 17:05 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decaling bones We are having the hardest time decaling bones. Femurs and tibias. Is there such as thing as overdecaling and the bone becoming hard again? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mark.webber <@t> nuigalway.ie Wed Apr 15 09:56:35 2009 From: mark.webber <@t> nuigalway.ie (Webber, Mark) Date: Wed Apr 15 09:59:13 2009 Subject: [Histonet] Suggestions for alternatives to Envision+ Message-ID: <6B017AD2AE2F6F489087FC986588136B07942D7E@EVS1.ac.nuigalway.ie> We are looking to use an amplification method for DAB manual staining and the DAKO envision+ had been recommended but as they are discontinuing the small pack sizes this would be of no use to us. Does anyone have experience with alternatives preferably from companies that are easily accessed from Europe. One product that seemed a possibility is Invitrogen/Zymed SuperPicture 3rd Gen, does anyone have any experience with this product. Thanks in advance for your help Mark Senior Technical Officer Department of Pathology National University of Ireland, Galway Clinical Science Institute Costello Road Galway From Rcartun <@t> harthosp.org Wed Apr 15 10:07:10 2009 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Apr 15 10:07:22 2009 Subject: [Histonet] Fli-1 antibody - Please help In-Reply-To: <002201c9bd77$1ace85f0$c680310a@cs.nsw.gov.au> References: <002201c9bd77$1ace85f0$c680310a@cs.nsw.gov.au> Message-ID: <49E5BFDF020000770000B050@gwmail4.harthosp.org> We are now using an IVD-labeled antibody from BioCare with good results. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Young Kwun" 4/14/2009 11:06 PM >>> Dear Histonetters, I was wondering whether anyone had success in Fli-1 immunohistochemistry on formalin-fixed, paraffin embedded human sections. I tried two polyclonal, commercial antibodies from abcam (ab15289) and Santa Cruz Biotech's Fli-1 (C-19, sc-356) without any positive stainings. Both antibodies were recommended for application on paraffin sections. Abcam's application notes even mentioned that perform enzymatic antigen retrieval BEFORE commencing with HIER procedure. The procedure still did not give any stains and the morphology was unacceptable. I tried with/without HIER with different buffers as well. I used primary dilutions 1:50 - 1:200 as recommended and I am using Leica's BondMax Autostainer. Any comments would be appreciated. Young Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From grudow1 <@t> jhmi.edu Wed Apr 15 11:01:55 2009 From: grudow1 <@t> jhmi.edu (Gay Rudow) Date: Wed Apr 15 11:02:01 2009 Subject: [Histonet] Freezing rodent brains for Mass Spec In-Reply-To: References: <75A0543E23D3A7458012D9E02EDBEC0002E8077AA0@UTHCMS1.uthouston.edu> Message-ID: <864A73846CE8F04D995603351682C29C871BA8E440@JHEMTEXVS2.win.ad.jhu.edu> We freeze mouse brains that have been perfused with 4% paraformaldehyde, put into PBS for 24 hours, and then into PBS with 30% sucrose overnight. We snap freeze them in methyl butane on dry ice. You have to put a thermometer into the methyl butane periodically to make sure that it doesn't get colder than -20 C or the brain will crack. Will the sucrose get in the way of what you are doing, or will this work for you? Gay Rudow -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Tuesday, April 14, 2009 6:54 PM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Freezing rodent brains for Mass Spec I have a colleague who requires snap frozen rodent brains to perform Mass Spec on 10micron frozen sections mounted on plates. Because OCT interferes with mass spec, they are currently using ethanol to slow the freezing process to prevent cracking of the brain in liquid nitrogen. The alcohol, however, causes some detriment to the surface of the brains - in science-speak "they get mushy". Does anyone have any suggestions for snap freezing brains without any substrate while preventing cracking? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From knemitz <@t> chpnet.org Wed Apr 15 11:26:50 2009 From: knemitz <@t> chpnet.org (Kyla Nemitz) Date: Wed Apr 15 11:27:40 2009 Subject: [Histonet] Histo Tech position in Brooklyn, NY - Long Island College Hospital Message-ID: <9DCA1D2278CBDA46936E075D0DB3E1741843C9@CHPMAIL1.AD.CHPNET.ORG> Hello Histonet! Long Island College Hospital (LICH), which serves as the hub of Continuum's services in Brooklyn, is a 516-bed teaching hospital located in the Brooklyn Heights/Cobble Hill section and is looking for a Histology Technician to join its team! The ideal candidate for this full-time position will have experience in Histology that includes: grossing, special staining and embedding methodologies. He/she will be able to process tissue samples from fixation to paraffin, as well as use a microtome to cut samples and prepare slides for staining. Qualified candidates for this position must have: - 2-3 years experience working in a clinical lab. - At least an Associates Degree, preferably in Medical Technology or related field. - A NYS certification as a Histology Technician or Technologist (or eligible). - Strong communication (written and verbal) skills. Additional Information: Interested candidates apply online to: www.chpnycareers.com Continuum Health Partners is the non-profit hospital system that supports Long Island College Hospital *Continuum Health Partners is committed to diversity and equal opportunity. **No Agency Recruiters please Feel free to contact me with any additional questions. Thank you! Kyla ****************** Kyla Nemitz Recruiter - Long Island College Hospital Continuum Health Partners 555 W. 57th St New York, NY 10019 Tel: (212)523-4050 Fax: (212)523-5776 knemitz@chpnet.org From ttruscot <@t> vetmed.wsu.edu Wed Apr 15 11:52:12 2009 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Wed Apr 15 11:52:19 2009 Subject: [Histonet] 5 Reasons - The list In-Reply-To: <5d9104a30904150653u165d35f2k47cba9d3d433e294@mail.gmail.com> References: <5d9104a30904150653u165d35f2k47cba9d3d433e294@mail.gmail.com> Message-ID: <44F1D6D7EB8CC84F92859EE5C4E6ECB42826B9D041@CVMMBX.vetmed.wsu.edu> Hi Cindy, Maybe the next 5 would be ; The pay is great, The bosses are very caring, The hours are flexible, The equipment is topnotch, The coworkers are brilliant. Have a great day, Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Wednesday, April 15, 2009 5:53 AM To: Histonet Subject: [Histonet] 5 Reasons - The list Thanks to so many of you I have come up with the following list: 1. You get to dig into peoples brains like no psychologist can. 2. Job security - no one else wants to work in the "human parts department". 3. It is never boring - "How did the patient get THAT in THERE?" 4. You get to do arts and crafts (Wax & Stains). 5. Most important - Providing quality care to patients. Cind Dubois Integrated Pathology Stockton, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed Apr 15 12:27:35 2009 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Apr 15 12:22:28 2009 Subject: [Histonet] 5 Reasons - The list In-Reply-To: <44F1D6D7EB8CC84F92859EE5C4E6ECB42826B9D041@CVMMBX.vetmed.wsu.edu> References: <5d9104a30904150653u165d35f2k47cba9d3d433e294@mail.gmail.com> <44F1D6D7EB8CC84F92859EE5C4E6ECB42826B9D041@CVMMBX.vetmed.wsu.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E390879CB9051@IBMB7Exchange.digestivespecialists.com> Tom, You must work at my facility! Those are all of the reasons here. (And that isn't said with tongue in cheek at all) Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Wednesday, April 15, 2009 12:52 PM To: 'Cindy DuBois'; Histonet Subject: RE: [Histonet] 5 Reasons - The list Hi Cindy, Maybe the next 5 would be ; The pay is great, The bosses are very caring, The hours are flexible, The equipment is topnotch, The coworkers are brilliant. Have a great day, Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Wednesday, April 15, 2009 5:53 AM To: Histonet Subject: [Histonet] 5 Reasons - The list Thanks to so many of you I have come up with the following list: 1. You get to dig into peoples brains like no psychologist can. 2. Job security - no one else wants to work in the "human parts department". 3. It is never boring - "How did the patient get THAT in THERE?" 4. You get to do arts and crafts (Wax & Stains). 5. Most important - Providing quality care to patients. Cind Dubois Integrated Pathology Stockton, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Marilyn.A.Weiss <@t> kp.org Wed Apr 15 12:51:58 2009 From: Marilyn.A.Weiss <@t> kp.org (Marilyn.A.Weiss@kp.org) Date: Wed Apr 15 12:51:13 2009 Subject: [Histonet] Marilyn A Weiss is out of the office as of 11:30 a.m. 4/15/2009 Message-ID: I will be out of the office starting 04/15/2009 and will not return until 04/20/2009. I will respond to your message when I return.In my absence please ask for Mary Campbell at 619-528-6801 if this is urgent. From jamie.erickson <@t> abbott.com Wed Apr 15 13:26:55 2009 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Wed Apr 15 13:27:04 2009 Subject: [Histonet] mouse tibia 200um sections-low speed saw? Message-ID: Hi Histonetters, Can any one help.... I am trying to repeat what I see in this paper and it said it cut cortical mouse bone tibia and I quote "For the cortical bone, a pair of 100?200 um-thick transverse sections were cut in the middle of the tibia with a low-speed diamond blade saw." I tried to cut this thin but I can't not get that thin... I am using a buehler isomet low speed saw with a 4" 15HC wafering blade. My micrometer reads every complete turn from zero to zero = 625um or 25 MIL and that is about as thin as I can cut mouse tibia. Please help am I missing something, what is the trick...?? Thanks Jamie _______________________________ Jamie Erickson Sr. Research Associate II M.S. HTL (ASCP) Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From jp1000r <@t> hotmail.com Wed Apr 15 13:26:19 2009 From: jp1000r <@t> hotmail.com (JP Rey) Date: Wed Apr 15 13:28:05 2009 Subject: [Histonet] Missouri Society for Histotechnology Annual Symposium Message-ID: The Missouri Society for Histotechnology will have its annual symposium hold at beautiful Lake of the Ozarks in a remarkable facility, Resort at Port Arrowhead from Thursday May 28th to Saturday May 30th, 2009. For more information check our website at: http://www.missouri-histo.org/index.cfm?p=4 &pid=4274&spid=4295 Or our video at: http://www.youtube.com/watch?v=6TgQAJ7J6UM We are looking forward meeting you there! Jean-Philippe REY MHT Vice President 4441 Gillham Road Kansas City, MO -64110 jp1000r@hotmail.com www.missouri-histo.org Tel.:(816) 213-2558 Tel.:(913) 945-6763 Fax.: (913) 660-0637 From jp1000r <@t> hotmail.com Wed Apr 15 13:30:03 2009 From: jp1000r <@t> hotmail.com (Jean-Philippe REY) Date: Wed Apr 15 13:30:08 2009 Subject: [Histonet] Missouri Society for Histotechnology 2009 Symposium Message-ID: The Missouri Society for Histotechnology will have its annual symposium hold at the beautiful Lake of the Ozarks in a remarkable facility, Resort at Port Arrowhead from Thursday May 28th to Saturday May 30th, 2009. To obtain more information: Check our website at: http://www.missouri-histo.org/index.cfm?p=4&pid=4274&spid=4295 Or our video at: http://www.youtube.com/watch?v=6TgQAJ7J6UM We are looking forward meeting you there! Jean-Philippe REY MHT Vice President 4441 Gillham Road Kansas City, MO -64110 jp1000r@hotmail.com www.missouri-histo.org Tel.:(816) 213-2558 Tel.:(913) 945-6763 Fax.: (913) 660-0637 From srishan <@t> mail.holyname.org Wed Apr 15 13:35:57 2009 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Wed Apr 15 13:36:29 2009 Subject: [Histonet] revalidation form for renewal of QIHC Message-ID: Hello All Does anyone know what website to use to print out the QIHC revalidation form inorder to renew the certification? Thanks Nirmala Srishan __________________ You can view the MSDS for CBG Biotech's "Formula 83" xylene substitute at www.cbgbiotech.com/msds/MSDS-Formula83071007.pdf It's described as a "naphthenic hydrocarbon blend". I'd note the low flash point (7 C, 45 F), considerably lower than xylene's. Other aliphatic or naphthenic (cycloalkane) mixtures offered as clearing agents have considerably higher flash points. Remember that if you want to recover it by distillation, you must make sure that your still has a distillation routine for it, and you cannot change clearing agents without changing the distillation routine, nor can you mix the solvents and recover them by distillation. If your lab manager shares cost information with you (in my experience most do not) then cost will certainly be a consideration, if it's indeed cheaper than xylene. According to the MSDS, disposal is done like any other aliphatic or naphthenic clearing agent. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Hospital is the recipient of: 2005, 2006, 2007 & 2008 Distinguished Hospital Awards for Clinical Excellence, HealthGrades 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades 2006 & 2007 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Primary Stroke Center Designation, The Joint Commission Chest Pain Center Accreditation , Society of Chest Pain Centers 2006, 2007 & 2008 Best Places to Work in New Jersey, NJBIZ The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or taking of any action in reliance on the content of this message is strictly prohibited. From anitaibsc <@t> aol.com Wed Apr 15 14:55:30 2009 From: anitaibsc <@t> aol.com (anitaibsc@aol.com) Date: Wed Apr 15 14:56:03 2009 Subject: [Histonet] Microtome and Human Tissue Blocks In-Reply-To: <3c7e700904151245q7a1f6de9k5f4f8d88dcfd90e7@mail.gmail.com> References: <3c7e700904151245q7a1f6de9k5f4f8d88dcfd90e7@mail.gmail.com> Message-ID: <8CB8C1B438FBE9A-E88-1248@webmail-mf05.sysops.aol.com> Hello Histonetters, We are looking for: 1) A used Microm Microtome, in good condition, either automated rotary or retracted rotary or other kind. 2) Human tissue blocks, Formalin-Fixed-Paraffin-Embedded (FFPE), several cancers and normal tissues. Thank you. Bader Sidikki, PhD R&D Director Email: baderbo@gmail.com Web site: www.ImmunoBioScience.com From RCazares <@t> schosp.org Wed Apr 15 15:11:49 2009 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Wed Apr 15 15:17:45 2009 Subject: [Histonet] Amyloid A- IHC stain Message-ID: <0672286797B07E40AA3414F534B7CB80040A84D7CA@EXCHCCRMB.schosp.org> Hello fellow Histonetters, I was wondering if anyone is running Biocare's Amyloid A or Amyloid P antibodies on Ventana's Benchmark. If so, are you using the concentrated or predilute antibody? Also, are you running these alone or with a Congo Red stain (as in a panel)? Any information is greatly appreciated. Thank you, Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 From stephanie.d.rivera <@t> gsk.com Wed Apr 15 15:24:28 2009 From: stephanie.d.rivera <@t> gsk.com (stephanie.d.rivera@gsk.com) Date: Wed Apr 15 15:24:50 2009 Subject: [Histonet] revalidation form for renewal of QIHC In-Reply-To: Message-ID: Hello, Go to ascp.org, click certification, icon get qualified(should be on the left), go down to Step # 7 Revalidate after 5 years click QIHC. Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 srishan@mail.holyname.org Sent by: histonet-bounces@lists.utsouthwestern.edu 15-Apr-2009 14:35 To histonet-bounces@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu cc Subject [Histonet] revalidation form for renewal of QIHC Hello All Does anyone know what website to use to print out the QIHC revalidation form inorder to renew the certification? Thanks Nirmala Srishan __________________ You can view the MSDS for CBG Biotech's "Formula 83" xylene substitute at www.cbgbiotech.com/msds/MSDS-Formula83071007.pdf It's described as a "naphthenic hydrocarbon blend". I'd note the low flash point (7 C, 45 F), considerably lower than xylene's. Other aliphatic or naphthenic (cycloalkane) mixtures offered as clearing agents have considerably higher flash points. Remember that if you want to recover it by distillation, you must make sure that your still has a distillation routine for it, and you cannot change clearing agents without changing the distillation routine, nor can you mix the solvents and recover them by distillation. If your lab manager shares cost information with you (in my experience most do not) then cost will certainly be a consideration, if it's indeed cheaper than xylene. According to the MSDS, disposal is done like any other aliphatic or naphthenic clearing agent. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Hospital is the recipient of: 2005, 2006, 2007 & 2008 Distinguished Hospital Awards for Clinical Excellence, HealthGrades 2008 Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades 2006 & 2007 Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Primary Stroke Center Designation, The Joint Commission Chest Pain Center Accreditation , Society of Chest Pain Centers 2006, 2007 & 2008 Best Places to Work in New Jersey, NJBIZ The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or taking of any action in reliance on the content of this message is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Wed Apr 15 15:39:24 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Apr 15 15:39:27 2009 Subject: [Histonet] container for undiluted bleach Message-ID: Hello again We keep a small squirt bottle of undiluted bleach in our hood but it's constantly eating through the plastic. Any ideas on other ways to store about 150 ml in a squirt bottle or what type of plastic is bleach-proof? The point here is that we want to store a small amount in the hood and possibly be able to squirt instead of pour it. Emily prometheus, thief of light, giver of light, bound by the gods, must have been a book. -mark danielewski, house of leaves From amylee779 <@t> yahoo.com Wed Apr 15 16:06:57 2009 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Wed Apr 15 16:07:00 2009 Subject: [Histonet] collagen III antibody Message-ID: <733879.19536.qm@web38007.mail.mud.yahoo.com> Hello, I?am?looking for a collagen III antibody that works on FFPE mouse tissue.?Could anybody?recommand one please? ? Thanks, Amy From putnamj <@t> ggclinic.com Wed Apr 15 17:48:19 2009 From: putnamj <@t> ggclinic.com (Putnam, Jodi) Date: Wed Apr 15 17:48:56 2009 Subject: [Histonet] prostate biopsy kits (LONG message sorry) Message-ID: Hi again. I am in need of information and pricing on prostate biopsy kits. We have just started taking on prostate biopsies. The kit that I am receiving holds 12 "20 ml" specimen bottles and they are made by Bostwick Labs. The urologists were sending them out to be processed but now that we have a pathology lab, it is in everyone's best interest to keep them in house. I love that we can give a quicker turnaround time for the doctors that are used to sending them out. My problem is this, once I use up all of the kits that the urologists have already bought, I will need my own. I need to keep the same order that they are used to, all of the 12 sites are preprinted below the hole that holds the specimen bottle in the box. (Maybe the list is a standard template used by all doctors now for prostate biopsies, I don't know.) I was going to try to come up with something myself to save money, homemade. I don't have time to shop around for the best price, but is it best for me to buy the kits? I just did a quick glance online and saw some kits (12 specimen) for 7.50/each. Is that good or can I do better? OR do any of you have an idea for the homemade version? I just need to make sure that my sites are exactly in the order that the doctors are used to seeing and 3 rows, left to right, top to bottom. I was going to reuse the boxes if they were not visibly contaminated and just put in 20 ml prefilled formalin bottles, but I am not sure that is a good thing. I worry about hygiene, cross contamination etc. Any help or words of wisdom would be great. If I had the time I would shop around but I have no spare time right now. I thought about getting something like the old plastic test tube racks (only with bigger holes) and putting solvent resistant labels on them and sanitizing them prior to returning to the urology dept. with new prefilled formalin bottles. Is that feasible? Any sales reps that are on the histonet, please email me any quotes on the kits. I am having a hard time fielding all the phone calls right now. Thanks so much. Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From dspears <@t> mmci.org Wed Apr 15 17:59:09 2009 From: dspears <@t> mmci.org (Dana Spears) Date: Wed Apr 15 17:59:33 2009 Subject: [Histonet] prostate biopsy kits (LONG message sorry) Message-ID: Jodi, I order my prostate kits from PATH-TEC. I met them at the NSH and they are such an awesome company! They customized the whole kit AND reqs for me. They even fold the reqs and pack them in the box! I can't say enough about this company-i was just amazed at the service and price. They really go above and beyond and can even set it up so your clients go online and just "order" your kits and they get billed to you. Good luck! Dana Spears, HTL Laboratory Manager Methodist Medical CTR 309.672.4930 (office) 309.255.7214 (cell) 309.279.3768 (fax) Sent from my Verizon Wireless BlackBerry -----Original Message----- From: "Putnam, Jodi" To: Sent: 4/15/2009 5:48:19 PM Subject: [Histonet] prostate biopsy kits (LONG message sorry) Hi again. I am in need of information and pricing on prostate biopsy kits. We have just started taking on prostate biopsies. The kit that I am receiving holds 12 "20 ml" specimen bottles and they are made by Bostwick Labs. The urologists were sending them out to be processed but now that we have a pathology lab, it is in everyone's best interest to keep them in house. I love that we can give a quicker turnaround time for the doctors that are used to sending them out. My problem is this, once I use up all of the kits that the urologists have already bought, I will need my own. I need to keep the same order that they are used to, all of the 12 sites are preprinted below the hole that holds the specimen bottle in the box. (Maybe the list is a standard template used by all doctors now for prostate biopsies, I don't know.) I was going to try to come up with something myself to save money, homemade. I don't have time to shop around for the best price, but is it best for me to buy the kits? I just did a quick glance online and saw some kits (12 specimen) for 7.50/each. Is that good or can I do better? OR do any of you have an idea for the homemade version? I just need to make sure that my sites are exactly in the order that the doctors are used to seeing and 3 rows, left to right, top to bottom. I was going to reuse the boxes if they were not visibly contaminated and just put in 20 ml prefilled formalin bottles, but I am not sure that is a good thing. I worry about hygiene, cross contamination etc. Any help or words of wisdom would be great. If I had the time I would shop around but I have no spare time right now. I thought about getting something like the old plastic test tube racks (only with bigger holes) and putting solvent resistant labels on them and sanitizing them prior to returning to the urology dept. with new prefilled formalin bottles. Is that feasible? Any sales reps that are on the histonet, please email me any quotes on the kits. I am having a hard time fielding all the phone calls right now. Thanks so much. Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. From AnthonyH <@t> chw.edu.au Wed Apr 15 18:15:16 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Apr 15 18:15:26 2009 Subject: [Histonet] container for undiluted bleach In-Reply-To: Message-ID: Why do need to store or use undiluted bleach? I would dilute it to a working solution and use that. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, 16 April 2009 6:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] container for undiluted bleach Hello again We keep a small squirt bottle of undiluted bleach in our hood but it's constantly eating through the plastic. Any ideas on other ways to store about 150 ml in a squirt bottle or what type of plastic is bleach-proof? The point here is that we want to store a small amount in the hood and possibly be able to squirt instead of pour it. Emily prometheus, thief of light, giver of light, bound by the gods, must have been a book. -mark danielewski, house of leaves _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From tissuearray <@t> hotmail.com Wed Apr 15 22:41:43 2009 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Wed Apr 15 22:41:52 2009 Subject: [Histonet] RE: TMAs for Cell Pellets - Histonet Digest, Vol 65, Issue 22 In-Reply-To: <49E49BB8.9090303@email.med.yale.edu> References: <200904091701.n39H1W3e015535@mr4.its.yale.edu> <49E49BB8.9090303@email.med.yale.edu> Message-ID: If you are making a cell blocks with your cell pellets then punching the cell block and inserting it into a TMA then is ideal. The cell pellets we have made were created like a cell block. The cells are spon down, the fluid is poured off and the cell button is mixed with a augar type substance (venders would have info on the augar type material used for making cell blocks). Once the solution solidifies it is placed in a paper or mesh bage and placed into a cassette for processing. Once processed the cells are imbedded into a paraffin block as you would tissue. A fresh H&E is cut off the block and areas of interest are marked on the slide to show the best spot to punch for the TMA. You will also find that the TMA instrument can pay for itself sometimes after one protocal. By this I mean you will save on antibody cost and have consistency in your staining since all samples are on one slide instead of many slides. I have constructed regular paraffin TMAs, cell arrays, sponge arrays (cells growing in a sponge type material processed and embedded in paraffin) and frozen TMAs. It just takes a little expermenting, some patience and pratice making TMAs. cheers, Thom > Date: Tue, 14 Apr 2009 10:20:40 -0400 > From: lac65@email.med.yale.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 22 > > Give me a shout in regards to cell pellets & TMA's. > There are several methods and some are less expensive then others. > Have a good one. > Lori > > > > Beecher is usually difficult to deal > with.histonet-request@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > > histonet@lists.utsouthwestern.edu > > > > To subscribe or unsubscribe via the World Wide Web, visit > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > or, via email, send a message with subject or body 'help' to > > histonet-request@lists.utsouthwestern.edu > > > > You can reach the person managing the list at > > histonet-owner@lists.utsouthwestern.edu > > > > When replying, please edit your Subject line so it is more specific > > than "Re: Contents of Histonet digest..." > > > > > > Today's Topics: > > > > 1. Re: TMA for cell pellets? (Bernie Taupin) > > 2. To all spam gourmets (V. Neubert) > > 3. RE: I recommend NOT opening that Attachment: (Hugh Luk) > > 4. RE: TMA for cell pellets? (Hartson, Louise) > > 5. Re: Formula 83 users? (Rene J Buesa) > > 6. Re: Negative IHC controls (Rene J Buesa) > > 7. RE: Formula 83 users? (Blazek, Linda) > > 8. Re: Spyware from an Attachment: (Geoff McAuliffe) > > 9. Formula 83-Thank you! (Jacqueline Farnsworth) > > 10. HISTOS 5 processor (FU,DONGTAO) > > 11. Position Open In Mass (Alyssa Peterson) > > 12. Re: HISTOS 5 processor (Robert Schoonhoven) > > 13. Travelling Histotechs (Robert Schoonhoven) > > 14. Regional Sales Manager position- West Coast > > (Kris.Caldwell@leica-microsystems.com) > > 15. New lab, need some info (Putnam, Jodi) > > > > > > ---------------------------------------------------------------------- > > > > Message: 1 > > Date: Wed, 8 Apr 2009 22:50:26 -0700 (PDT) > > From: Bernie Taupin > > Subject: Re: [Histonet] TMA for cell pellets? > > To: Thom Jensen , > > louise_hartson@urmc.rochester.edu, histonet@lists.utsouthwestern.edu > > Message-ID: <338565.20208.qm@web43513.mail.sp1.yahoo.com> > > Content-Type: text/plain; charset=iso-8859-1 > > > > Recently, someone mentioned putting a library of protocols online, perhaps in the HistoNet archives. I'd like to second that emotion if its possible to make happen... What a great resource that would be! > > > > > > > > > > ________________________________ > > From: Thom Jensen > > To: louise_hartson@urmc.rochester.edu; histonet@lists.utsouthwestern.edu > > Sent: Thursday, April 9, 2009 1:27:18 AM > > Subject: [Histonet] TMA for cell pellets? > > > > > > > > Have you tried to embed your cell pellets into a tissue microarray? Two companies I would recommend: > > www.arraymold.com > > www.beecherinstruments.com > > > > Two of the best TMA products on the market. > > > > > > cheers, > > > > > >> Date: Thu, 2 Apr 2009 12:05:39 -0400 > >> From: Louise_Hartson@URMC.Rochester.edu > >> To: histonet@lists.utsouthwestern.edu > >> Subject: [Histonet] Embedding cell pellets > >> > >> > >> I am looking for a protocol for embedding cell pellets in paraffin. > >> Thanks, > >> Louise > >> > >> Louise Hartson, BA > >> Senior Technical Associate > >> University of Rochester > >> Louise_Hartson@URMC.Rochester.edu > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > _________________________________________________________________ > > Hotmail? is up to 70% faster. Now good news travels really fast. > > http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009_______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > ------------------------------ > > > > Message: 2 > > Date: Thu, 09 Apr 2009 10:07:54 +0200 > > From: "V. Neubert" > > Subject: [Histonet] To all spam gourmets > > To: histonet@lists.utsouthwestern.edu > > Message-ID: <49DDACDA.8040503@vneubert.com> > > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > > > Please, stop spamming this list. > > Comment if you know something useful. Otherwise keep it. > > I don't mind what you had for breakfast in June 1st last year or your > > opinion on my clothes' colour. > > > > Believe it or not, there IS a real life ( link: > > http://en.wikipedia.org/wiki/Real_life_(reality) ), and maybe you should > > check it out - it has way more to offer than waiting for responses to > > mock about. > > > > Thanks. > > Valentin > > > > PS: What about a moderated board? Every inappropriate comment on HISTO > > TOPIC could be moved or even deleted, except in the spamming forum which > > can be found on nearly every board on the net. > > > > > > > > ------------------------------ > > > > Message: 3 > > Date: Wed, 8 Apr 2009 22:13:22 -1000 > > From: Hugh Luk > > Subject: RE: [Histonet] I recommend NOT opening that Attachment: > > To: histonet > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > Fellow histology and related fields professionals, > > > > > > > > > > > > > > > > > > > > > > > > I'm not opening this. From a call name of "u know (u_deserve_2_know@yahoo.com)." The last time I opened something like this, from a name like this, I ended up owing money for Viagra treatments for Panda bears or something like that. It may be real, but it probably is spam. There...has been...a lot...of it...recently! > > > > By the way, there is no such thing as a "Virus-proof LINK!" Not sure? Just Google it. > > > > If you need Spyware or Antivirus software, check your IT department. Also, CNET has free downloads of AdAware, or AVG antivirus 8. > > > > And now for a joke (I think we all need it); A physician is doing his rounds and a nurse stops him in the hallway, "Doctor, can you sign these reports?" "Fine" he says and proceeds to pull out a rectal thermometer from his coat pocket. He looks at it in disbelief and exclaims, "Darn it! Some a$$ has my pen!" > > > > Respectfully, > > Hugh Luk, HTL (ASCP) > > Cancer Research center of Hawaii > > Pathology shared resources lab manager > > KP- Hawaii > > > > > > > > > >> Date: Wed, 8 Apr 2009 21:41:35 -0700 > >> From: u_deserve_2_know@yahoo.com > >> To: histonet@lists.utsouthwestern.edu > >> Subject: [Histonet] Spyware from an Attachment: > >> > >> Dear Listers, > >> > >> If you ever opened any strange attachments from this list, you should really consider scanning your Hard Disk for spyware. There is a great free anti-spyware program we use, which you can get at http://DELETED<----that is a virus-proof link, by the way. > >> > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > _________________________________________________________________ > > Quick access to your favorite MSN content and Windows Live with Internet Explorer 8. > > http://ie8.msn.com/microsoft/internet-explorer-8/en-us/ie8.aspx?ocid=B037MSN55C0701A > > > > ------------------------------ > > > > Message: 4 > > Date: Thu, 9 Apr 2009 08:29:23 -0400 > > From: "Hartson, Louise" > > Subject: RE: [Histonet] TMA for cell pellets? > > To: "Bernie Taupin" , "Thom Jensen" > > , > > Message-ID: > > <1089A756B010074CA09E65CD92401608018A8CAC@MEDMAIL.urmc-sh.rochester.edu> > > > > Content-Type: text/plain; charset="iso-8859-1" > > > > Thank you to everyone who responded!! I was given the cell pellets already in the matrix so I only had to process!! It was easier than the mouse tissues!! Since we do so few 20-40 at a time I do everything by hand...no automation in our lab!! > > > > > > -----Original Message----- > > From: Bernie Taupin [mailto:bernietaupin@ymail.com] > > Sent: Thu 4/9/2009 1:50 AM > > To: Thom Jensen; Hartson, Louise; histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] TMA for cell pellets? > > > > Recently, someone mentioned putting a library of protocols online, perhaps in the HistoNet archives. I'd like to second that emotion if its possible to make happen... What a great resource that would be! > > > > > > > > > > ________________________________ > > From: Thom Jensen > > To: louise_hartson@urmc.rochester.edu; histonet@lists.utsouthwestern.edu > > Sent: Thursday, April 9, 2009 1:27:18 AM > > Subject: [Histonet] TMA for cell pellets? > > > > > > > > Have you tried to embed your cell pellets into a tissue microarray? Two companies I would recommend: > > www.arraymold.com > > www.beecherinstruments.com > > > > Two of the best TMA products on the market. > > > > > > cheers, > > > > > >> Date: Thu, 2 Apr 2009 12:05:39 -0400 > >> From: Louise_Hartson@URMC.Rochester.edu > >> To: histonet@lists.utsouthwestern.edu > >> Subject: [Histonet] Embedding cell pellets > >> > >> > >> I am looking for a protocol for embedding cell pellets in paraffin. > >> Thanks, > >> Louise > >> > >> Louise Hartson, BA > >> Senior Technical Associate > >> University of Rochester > >> Louise_Hartson@URMC.Rochester.edu > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > _________________________________________________________________ > > Hotmail? is up to 70% faster. Now good news travels really fast. > > http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009_______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > > > > > ------------------------------ > > > > Message: 5 > > Date: Thu, 9 Apr 2009 06:56:21 -0700 (PDT) > > From: Rene J Buesa > > Subject: Re: [Histonet] Formula 83 users? > > To: Histonet , Jacqueline > > Farnsworth > > Message-ID: <613905.80912.qm@web65715.mail.ac4.yahoo.com> > > Content-Type: text/plain; charset=iso-8859-1 > > > > Jacqueline: > > Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant. > > Ren? J. > > > > --- On Wed, 4/8/09, Jacqueline Farnsworth wrote: > > > > From: Jacqueline Farnsworth > > Subject: [Histonet] Formula 83 users? > > To: "Histonet" > > Date: Wednesday, April 8, 2009, 12:31 PM > > > > HI all. > > I have searched the Histonet archives and found a lot of very positive reviews > > regarding Formula 83. I am wondering if anyone has encountered any issues with > > using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there > > any miscibility issues? Are you soaking in xylene prior to tape coverslipping > > required? Any helpful hints/tips/tricks? > > > > Thank you in advance. > > > > > > Jacqueline Farnsworth > > Anatomic Pathology, Tech III > > Foothills Medical Centre > > Calgary Laboratory Services > > > > Ph: 403-944-1578 > > Fax: 403-944-4748 > > P Please consider the environment before printing this email. > > > > ________________________________ > > This message and any attached documents are only for the use of the intended > > recipient(s), are confidential and may contain privileged information. Any > > unauthorized review, use, retransmission, or other disclosure is strictly > > prohibited. If you have received this message in error, please notify the sender > > immediately, and then delete the original message. Thank you. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > ------------------------------ > > > > Message: 6 > > Date: Thu, 9 Apr 2009 06:58:45 -0700 (PDT) > > From: Rene J Buesa > > Subject: Re: [Histonet] Negative IHC controls > > To: histonet@lists.utsouthwestern.edu, Sheila Haas > > > > Message-ID: <292901.13223.qm@web65712.mail.ac4.yahoo.com> > > Content-Type: text/plain; charset=iso-8859-1 > > > > I used to run a negative control per case or per block within any given case when doing IHC, but not for reagents. > > Ren? J. > > > > --- On Wed, 4/8/09, Sheila Haas wrote: > > > > From: Sheila Haas > > Subject: [Histonet] Negative IHC controls > > To: histonet@lists.utsouthwestern.edu > > Date: Wednesday, April 8, 2009, 2:57 PM > > > > I have a question concerning ANP.22570 on the CAP checklist. Could you all > > tell me how you are handling > > negative controls for IHC staining? The question actually states (and I've > > confirmed with CAP) that we should be running two types of negative controls. > > One for reagents and one for each antibody in a run. I'd like to know > > what the practice is. This seems very costly and time consuming. Thanks in > > advance! > > > > Sheila Haas > > Laboratory Supervisor > > Micro Path Laboratories > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > ------------------------------ > > > > Message: 7 > > Date: Thu, 9 Apr 2009 10:14:48 -0400 > > From: "Blazek, Linda" > > Subject: RE: [Histonet] Formula 83 users? > > To: "'rjbuesa@yahoo.com'" , Histonet > > , Jacqueline Farnsworth > > > > Message-ID: > > <5A2BD13465E061429D6455C8D6B40E3908778D156E@IBMB7Exchange.digestivespecialists.com> > > > > Content-Type: text/plain; charset="iso-8859-1" > > > > > > With all due respect Ren?; I have been using Formula 83 with my coverslipper with no problems at all though have a glass coverslipper not a tape. > > Linda > > > > > > Linda Blazek HT (ASCP) > > Manager/Supervisor > > GI Pathology of Dayton > > 7415 Brandt Pike > > Huber Heights, OH 45424 > > Phone: (937) 293-4424 ext 7118 > > Email: lblazek@digestivespecialists.com > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > > Sent: Thursday, April 09, 2009 9:56 AM > > To: Histonet; Jacqueline Farnsworth > > Subject: Re: [Histonet] Formula 83 users? > > > > Jacqueline: > > Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant. > > Ren? J. > > > > --- On Wed, 4/8/09, Jacqueline Farnsworth wrote: > > > > From: Jacqueline Farnsworth > > Subject: [Histonet] Formula 83 users? > > To: "Histonet" > > Date: Wednesday, April 8, 2009, 12:31 PM > > > > HI all. > > I have searched the Histonet archives and found a lot of very positive reviews > > regarding Formula 83. I am wondering if anyone has encountered any issues with > > using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there > > any miscibility issues? Are you soaking in xylene prior to tape coverslipping > > required? Any helpful hints/tips/tricks? > > > > Thank you in advance. > > > > > > Jacqueline Farnsworth > > Anatomic Pathology, Tech III > > Foothills Medical Centre > > Calgary Laboratory Services > > > > Ph: 403-944-1578 > > Fax: 403-944-4748 > > P Please consider the environment before printing this email. > > > > ________________________________ > > This message and any attached documents are only for the use of the intended > > recipient(s), are confidential and may contain privileged information. Any > > unauthorized review, use, retransmission, or other disclosure is strictly > > prohibited. If you have received this message in error, please notify the sender > > immediately, and then delete the original message. Thank you. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > > > Message: 8 > > Date: Thu, 09 Apr 2009 10:11:11 -0400 > > From: Geoff McAuliffe > > Subject: Re: [Histonet] Spyware from an Attachment: > > To: u know > > Cc: histonet@lists.utsouthwestern.edu > > Message-ID: <49DE01FF.100@umdnj.edu> > > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > > > Dear List: > > > > I strongly suggest that you NOT use any links offered by people with no > > name and no affiliation. Yes, there are good spyware detection programs > > available at no cost (Adaware for example) but I don't think this is one > > of them. There are at least 3 potential saboteurs on this list. > > > > Geoff > > > > u know wrote: > > > >> Dear Listers, > >> > >> If you ever opened any strange attachments from this list, you should really consider scanning your Hard Disk for spyware. There is a great free anti-spyware program we use, which you can get at http://tinyurl.com/ynupj4 <----that is a virus-proof link, by the way. > >> > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >> > >> > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Rediscover Hotmail?: Now available on your iPhone or BlackBerry http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Mobile1_042009 From carl.hobbs <@t> kcl.ac.uk Thu Apr 16 04:12:30 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Thu Apr 16 04:14:34 2009 Subject: [Histonet] re:Leica TP 1020 processor Message-ID: <11D9615B89C10747B1C985966A63D7CA2961FE0D46@KCL-MAIL04.kclad.ds.kcl.ac.uk> I have one: bought it 5yrs ago. It sits on an open bench. The perspex skirt and the internal extract fan venting via a xylene filter ( formalin one too, if you use it, on the machine) prevent smells, I am assured ( well, having worked as a histologist for 30yrs, I probably can't smell it ;-). One can vent it into a fume hood, via tubing. Not a heavily used machine ( x3 runs /week). I like it very much. No problems. In my previous post, I also purchased one and used it for the same time/usage with never a problem. Carl From ree3 <@t> leicester.ac.uk Thu Apr 16 05:22:38 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Apr 16 05:22:45 2009 Subject: [Histonet] 5 Reasons - The list In-Reply-To: <44F1D6D7EB8CC84F92859EE5C4E6ECB42826B9D041@CVMMBX.vetmed.wsu.edu> References: <5d9104a30904150653u165d35f2k47cba9d3d433e294@mail.gmail.com> <44F1D6D7EB8CC84F92859EE5C4E6ECB42826B9D041@CVMMBX.vetmed.wsu.edu> Message-ID: <7722595275A4DD4FA225B92CDBF174A17457783CED@EXC-MBX3.cfs.le.ac.uk> The 11th reason might be that one can possess a knowing smile when watching Quincy or CSI!. --Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: 15 April 2009 17:52 To: 'Cindy DuBois'; Histonet Subject: RE: [Histonet] 5 Reasons - The list Hi Cindy, Maybe the next 5 would be ; The pay is great, The bosses are very caring, The hours are flexible, The equipment is topnotch, The coworkers are brilliant. Have a great day, Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Wednesday, April 15, 2009 5:53 AM To: Histonet Subject: [Histonet] 5 Reasons - The list Thanks to so many of you I have come up with the following list: 1. You get to dig into peoples brains like no psychologist can. 2. Job security - no one else wants to work in the "human parts department". 3. It is never boring - "How did the patient get THAT in THERE?" 4. You get to do arts and crafts (Wax & Stains). 5. Most important - Providing quality care to patients. Cind Dubois Integrated Pathology Stockton, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Apr 16 08:07:32 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 16 08:07:37 2009 Subject: [Histonet] prostate biopsy kits (LONG message sorry) In-Reply-To: Message-ID: <936930.85687.qm@web65701.mail.ac4.yahoo.com> First get the bottles at the best price you can. After that put them in the order you are going to use them to determine the surface area?they?will occupy. Knowing that surface buy several pieces of solid hard wood and bore the holes to fit the bottles you have. Identify the holes and apply plastic resin to all the pieces. The plastic will prevent the numbers to be washed out and will allow you to sanitize the wood. Make as many as cases your office will have per day. Ren? J.?? --- On Wed, 4/15/09, Putnam, Jodi wrote: From: Putnam, Jodi Subject: [Histonet] prostate biopsy kits (LONG message sorry) To: histonet@lists.utsouthwestern.edu Date: Wednesday, April 15, 2009, 6:48 PM Hi again. I am in need of information and pricing on prostate biopsy kits. We have just started taking on prostate biopsies. The kit that I am receiving holds 12 "20 ml" specimen bottles and they are made by Bostwick Labs. The urologists were sending them out to be processed but now that we have a pathology lab, it is in everyone's best interest to keep them in house. I love that we can give a quicker turnaround time for the doctors that are used to sending them out. My problem is this, once I use up all of the kits that the urologists have already bought, I will need my own. I need to keep the same order that they are used to, all of the 12 sites are preprinted below the hole that holds the specimen bottle in the box. (Maybe the list is a standard template used by all doctors now for prostate biopsies, I don't know.) I was going to try to come up with something myself to save money, homemade. I don't have time to shop around for the best price, but is it best for me to buy the kits? I just did a quick glance online and saw some kits (12 specimen) for 7.50/each. Is that good or can I do better? OR do any of you have an idea for the homemade version? I just need to make sure that my sites are exactly in the order that the doctors are used to seeing and 3 rows, left to right, top to bottom. I was going to reuse the boxes if they were not visibly contaminated and just put in 20 ml prefilled formalin bottles, but I am not sure that is a good thing. I worry about hygiene, cross contamination etc. Any help or words of wisdom would be great. If I had the time I would shop around but I have no spare time right now. I thought about getting something like the old plastic test tube racks (only with bigger holes) and putting solvent resistant labels on them and sanitizing them prior to returning to the urology dept. with new prefilled formalin bottles. Is that feasible? Any sales reps that are on the histonet, please email me any quotes on the kits. I am having a hard time fielding all the phone calls right now. Thanks so much. Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Apr 16 09:06:27 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 16 09:06:31 2009 Subject: [Histonet] PROCEDURES/PROTOCOLS Message-ID: <25806.61629.qm@web65703.mail.ac4.yahoo.com> On Richard Yeo's behalf. --- On Thu, 4/16/09, Richard Yeo wrote: From: Richard Yeo Subject: RE: PROCEDURES/PROTOCOLS To: rjbuesa@yahoo.com Date: Thursday, April 16, 2009, 9:46 AM Rene, can you please ask on histo-net about this for me? I tried several times and it won?t go through. ? Thanks I really appreciate it.???????????? Rich Y --- On Wed, 4/15/09, Richard Yeo wrote: From: Richard Yeo ryeo@wchosp.org My pathologist wants me to get some so he can train 1 or 2 techs that do qualify to do grossing. ? I would greatly appreciate any help you can give me ? ?????????????? ???????????????????????????????????????????????Thanks Rich Y ? Richard E Yeo HT(ASCP) Histology Supervisor Wooster Community Hospital Wooster Ohio ryeo@wchosp.org PRIVILEGE AND CONFIDENTIALITY NOTICE The information in this electronic mail is intended for the named recipients only. It may contain privileged and confidential material and may be protected under law by the Health Insurance Portability and Accountability Act. Any use of this information by anyone other than the intended receiver is prohibited. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately by replying to this electronic e-mail. Please delete it from your computer. Thank you ? PRIVILEGE AND CONFIDENTIALITY NOTICE The information in this electronic mail is intended for the named recipients only. It may contain privileged and confidential material and may be protected under law by the Health Insurance Portability and Accountability Act. Any use of this information by anyone other than the intended receiver is prohibited. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately by replying to this electronic e-mail. Please delete it from your computer. Thank you From lac65 <@t> email.med.yale.edu Thu Apr 16 09:11:49 2009 From: lac65 <@t> email.med.yale.edu (Lori Charette) Date: Thu Apr 16 09:12:12 2009 Subject: [Histonet] Re: TMAs for Cell Pellets - Histonet Digest, Vol 65, Issue 22 In-Reply-To: References: <200904091701.n39H1W3e015535@mr4.its.yale.edu> <49E49BB8.9090303@email.med.yale.edu> Message-ID: <49E73CA5.7050704@email.med.yale.edu> Cell pellet density is important and the medium in which the cells are suspended in (i.e. agar, etc...) makes a difference as well. The medium consistency and type is important to the cell block preparation if they are to be used in a TMA. This is true when cutting tissue micro-arrays that contain both cell pellets and tissue samples. If you are looking to make TMA's of cell-pellets, tissue, or both on a regular basis then perhaps purchasing an arrayer is the way to go but for making control TMA's or TMA's of interest then there are other avenues. Lori Thom Jensen wrote: > If you are making a cell blocks with your cell pellets then punching > the cell block and inserting it into a TMA then is ideal. The cell > pellets we have made were created like a cell block. The cells are > spon down, the fluid is poured off and the cell button is mixed with a > augar type substance (venders would have info on the augar type > material used for making cell blocks). Once the solution solidifies > it is placed in a paper or mesh bage and placed into a cassette for > processing. Once processed the cells are imbedded into a paraffin > block as you would tissue. A fresh H&E is cut off the block and areas > of interest are marked on the slide to show the best spot to punch for > the TMA. You will also find that the TMA instrument can pay for itself > sometimes after one protocal. By this I mean you will save on > antibody cost and have consistency in your staining since all samples > are on one slide instead of many slides. I have constructed regular > paraffin TMAs, cell arrays, sponge arrays (cells growing in a sponge > type material processed and embedded in paraffin) and frozen TMAs. It > just takes a little expermenting, some patience and pratice making TMAs. > > cheers, > Thom > > > > Date: Tue, 14 Apr 2009 10:20:40 -0400 > > From: lac65@email.med.yale.edu > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Re: Histonet Digest, Vol 65, Issue 22 > > > > Give me a shout in regards to cell pellets & TMA's. > > There are several methods and some are less expensive then others. > > Have a good one. > > Lori > > > > > > > > Beecher is usually difficult to deal > > with.histonet-request@lists.utsouthwestern.edu wrote: > > > Send Histonet mailing list submissions to > > > histonet@lists.utsouthwestern.edu > > > > > > To subscribe or unsubscribe via the World Wide Web, visit > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > or, via email, send a message with subject or body 'help' to > > > histonet-request@lists.utsouthwestern.edu > > > > > > You can reach the person managing the list at > > > histonet-owner@lists.utsouthwestern.edu > > > > > > When replying, please edit your Subject line so it is more specific > > > than "Re: Contents of Histonet digest..." > > > > > > > > > Today's Topics: > > > > > > 1. Re: TMA for cell pellets? (Bernie Taupin) > > > 2. To all spam gourmets (V. Neubert) > > > 3. RE: I recommend NOT opening that Attachment: (Hugh Luk) > > > 4. RE: TMA for cell pellets? (Hartson, Louise) > > > 5. Re: Formula 83 users? (Rene J Buesa) > > > 6. Re: Negative IHC controls (Rene J Buesa) > > > 7. RE: Formula 83 users? (Blazek, Linda) > > > 8. Re: Spyware from an Attachment: (Geoff McAuliffe) > > > 9. Formula 83-Thank you! (Jacqueline Farnsworth) > > > 10. HISTOS 5 processor (FU,DONGTAO) > > > 11. Position Open In Mass (Alyssa Peterson) > > > 12. Re: HISTOS 5 processor (Robert Schoonhoven) > > > 13. Travelling Histotechs (Robert Schoonhoven) > > > 14. Regional Sales Manager position- West Coast > > > (Kris.Caldwell@leica-microsystems.com) > > > 15. New lab, need some info (Putnam, Jodi) > > > > > > > > > ---------------------------------------------------------------------- > > > > > > Message: 1 > > > Date: Wed, 8 Apr 2009 22:50:26 -0700 (PDT) > > > From: Bernie Taupin > > > Subject: Re: [Histonet] TMA for cell pellets? > > > To: Thom Jensen , > > > louise_hartson@urmc.rochester.edu, histonet@lists.utsouthwestern.edu > > > Message-ID: <338565.20208.qm@web43513.mail.sp1.yahoo.com> > > > Content-Type: text/plain; charset=iso-8859-1 > > > > > > Recently, someone mentioned putting a library of protocols online, > perhaps in the HistoNet archives. I'd like to second that emotion if > its possible to make happen... What a great resource that would be! > > > > > > > > > > > > > > > ________________________________ > > > From: Thom Jensen > > > To: louise_hartson@urmc.rochester.edu; > histonet@lists.utsouthwestern.edu > > > Sent: Thursday, April 9, 2009 1:27:18 AM > > > Subject: [Histonet] TMA for cell pellets? > > > > > > > > > > > > Have you tried to embed your cell pellets into a tissue > microarray? Two companies I would recommend: > > > www.arraymold.com > > > www.beecherinstruments.com > > > > > > Two of the best TMA products on the market. > > > > > > > > > cheers, > > > > > > > > >> Date: Thu, 2 Apr 2009 12:05:39 -0400 > > >> From: Louise_Hartson@URMC.Rochester.edu > > >> To: histonet@lists.utsouthwestern.edu > > >> Subject: [Histonet] Embedding cell pellets > > >> > > >> > > >> I am looking for a protocol for embedding cell pellets in paraffin. > > >> Thanks, > > >> Louise > > >> > > >> Louise Hartson, BA > > >> Senior Technical Associate > > >> University of Rochester > > >> Louise_Hartson@URMC.Rochester.edu > > >> _______________________________________________ > > >> Histonet mailing list > > >> Histonet@lists.utsouthwestern.edu > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >> > > > > > > _________________________________________________________________ > > > Hotmail? is up to 70% faster. Now good news travels really fast. > > > > http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009_______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > > > > > > > ------------------------------ > > > > > > Message: 2 > > > Date: Thu, 09 Apr 2009 10:07:54 +0200 > > > From: "V. Neubert" > > > Subject: [Histonet] To all spam gourmets > > > To: histonet@lists.utsouthwestern.edu > > > Message-ID: <49DDACDA.8040503@vneubert.com> > > > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > > > > > Please, stop spamming this list. > > > Comment if you know something useful. Otherwise keep it. > > > I don't mind what you had for breakfast in June 1st last year or your > > > opinion on my clothes' colour. > > > > > > Believe it or not, there IS a real life ( link: > > > http://en.wikipedia.org/wiki/Real_life_(reality) ), and maybe you > should > > > check it out - it has way more to offer than waiting for responses to > > > mock about. > > > > > > Thanks. > > > Valentin > > > > > > PS: What about a moderated board? Every inappropriate comment on > HISTO > > > TOPIC could be moved or even deleted, except in the spamming forum > which > > > can be found on nearly every board on the net. > > > > > > > > > > > > ------------------------------ > > > > > > Message: 3 > > > Date: Wed, 8 Apr 2009 22:13:22 -1000 > > > From: Hugh Luk > > > Subject: RE: [Histonet] I recommend NOT opening that Attachment: > > > To: histonet > > > Message-ID: > > > Content-Type: text/plain; charset="iso-8859-1" > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > Fellow histology and related fields professionals, > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > I'm not opening this. From a call name of "u know > (u_deserve_2_know@yahoo.com)." The last time I opened something like > this, from a name like this, I ended up owing money for Viagra > treatments for Panda bears or something like that. It may be real, but > it probably is spam. There...has been...a lot...of it...recently! > > > > > > By the way, there is no such thing as a "Virus-proof LINK!" Not > sure? Just Google it. > > > > > > If you need Spyware or Antivirus software, check your IT > department. Also, CNET has free downloads of AdAware, or AVG antivirus 8. > > > > > > And now for a joke (I think we all need it); A physician is doing > his rounds and a nurse stops him in the hallway, "Doctor, can you sign > these reports?" "Fine" he says and proceeds to pull out a rectal > thermometer from his coat pocket. He looks at it in disbelief and > exclaims, "Darn it! Some a$$ has my pen!" > > > > > > Respectfully, > > > Hugh Luk, HTL (ASCP) > > > Cancer Research center of Hawaii > > > Pathology shared resources lab manager > > > KP- Hawaii > > > > > > > > > > > > > > >> Date: Wed, 8 Apr 2009 21:41:35 -0700 > > >> From: u_deserve_2_know@yahoo.com > > >> To: histonet@lists.utsouthwestern.edu > > >> Subject: [Histonet] Spyware from an Attachment: > > >> > > >> Dear Listers, > > >> > > >> If you ever opened any strange attachments from this list, you > should really consider scanning your Hard Disk for spyware. There is a > great free anti-spyware program we use, which you can get at > http://DELETED<----that is a virus-proof link, by the way. > > >> > > >> > > >> > > >> > > >> _______________________________________________ > > >> Histonet mailing list > > >> Histonet@lists.utsouthwestern.edu > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >> > > > > > > _________________________________________________________________ > > > Quick access to your favorite MSN content and Windows Live with > Internet Explorer 8. > > > > http://ie8.msn.com/microsoft/internet-explorer-8/en-us/ie8.aspx?ocid=B037MSN55C0701A > > > > > > ------------------------------ > > > > > > Message: 4 > > > Date: Thu, 9 Apr 2009 08:29:23 -0400 > > > From: "Hartson, Louise" > > > Subject: RE: [Histonet] TMA for cell pellets? > > > To: "Bernie Taupin" , "Thom Jensen" > > > , > > > Message-ID: > > > > <1089A756B010074CA09E65CD92401608018A8CAC@MEDMAIL.urmc-sh.rochester.edu> > > > > > > Content-Type: text/plain; charset="iso-8859-1" > > > > > > Thank you to everyone who responded!! I was given the cell pellets > already in the matrix so I only had to process!! It was easier than > the mouse tissues!! Since we do so few 20-40 at a time I do everything > by hand...no automation in our lab!! > > > > > > > > > -----Original Message----- > > > From: Bernie Taupin [mailto:bernietaupin@ymail.com] > > > Sent: Thu 4/9/2009 1:50 AM > > > To: Thom Jensen; Hartson, Louise; histonet@lists.utsouthwestern.edu > > > Subject: Re: [Histonet] TMA for cell pellets? > > > > > > Recently, someone mentioned putting a library of protocols online, > perhaps in the HistoNet archives. I'd like to second that emotion if > its possible to make happen... What a great resource that would be! > > > > > > > > > > > > > > > ________________________________ > > > From: Thom Jensen > > > To: louise_hartson@urmc.rochester.edu; > histonet@lists.utsouthwestern.edu > > > Sent: Thursday, April 9, 2009 1:27:18 AM > > > Subject: [Histonet] TMA for cell pellets? > > > > > > > > > > > > Have you tried to embed your cell pellets into a tissue > microarray? Two companies I would recommend: > > > www.arraymold.com > > > www.beecherinstruments.com > > > > > > Two of the best TMA products on the market. > > > > > > > > > cheers, > > > > > > > > >> Date: Thu, 2 Apr 2009 12:05:39 -0400 > > >> From: Louise_Hartson@URMC.Rochester.edu > > >> To: histonet@lists.utsouthwestern.edu > > >> Subject: [Histonet] Embedding cell pellets > > >> > > >> > > >> I am looking for a protocol for embedding cell pellets in paraffin. > > >> Thanks, > > >> Louise > > >> > > >> Louise Hartson, BA > > >> Senior Technical Associate > > >> University of Rochester > > >> Louise_Hartson@URMC.Rochester.edu > > >> _______________________________________________ > > >> Histonet mailing list > > >> Histonet@lists.utsouthwestern.edu > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >> > > > > > > _________________________________________________________________ > > > Hotmail? is up to 70% faster. Now good news travels really fast. > > > > http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_70faster_032009_______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > > > > > > > > > > > > > ------------------------------ > > > > > > Message: 5 > > > Date: Thu, 9 Apr 2009 06:56:21 -0700 (PDT) > > > From: Rene J Buesa > > > Subject: Re: [Histonet] Formula 83 users? > > > To: Histonet , Jacqueline > > > Farnsworth > > > Message-ID: <613905.80912.qm@web65715.mail.ac4.yahoo.com> > > > Content-Type: text/plain; charset=iso-8859-1 > > > > > > Jacqueline: > > > Formula 83 is a naphthenic hydrocarbon that cannot be used with > coverslippers, is recyclable and while some people have been using it > for more than 20 years, others find it irritant. > > > Ren? J. > > > > > > --- On Wed, 4/8/09, Jacqueline Farnsworth > wrote: > > > > > > From: Jacqueline Farnsworth > > > Subject: [Histonet] Formula 83 users? > > > To: "Histonet" > > > Date: Wednesday, April 8, 2009, 12:31 PM > > > > > > HI all. > > > I have searched the Histonet archives and found a lot of very > positive reviews > > > regarding Formula 83. I am wondering if anyone has encountered any > issues with > > > using Formula 83 on a stainer and then xylene on a tape > coverslipper. Are there > > > any miscibility issues? Are you soaking in xylene prior to tape > coverslipping > > > required? Any helpful hints/tips/tricks? > > > > > > Thank you in advance. > > > > > > > > > Jacqueline Farnsworth > > > Anatomic Pathology, Tech III > > > Foothills Medical Centre > > > Calgary Laboratory Services > > > > > > Ph: 403-944-1578 > > > Fax: 403-944-4748 > > > P Please consider the environment before printing this email. > > > > > > ________________________________ > > > This message and any attached documents are only for the use of > the intended > > > recipient(s), are confidential and may contain privileged > information. Any > > > unauthorized review, use, retransmission, or other disclosure is > strictly > > > prohibited. If you have received this message in error, please > notify the sender > > > immediately, and then delete the original message. Thank you. > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > > > > > > > ------------------------------ > > > > > > Message: 6 > > > Date: Thu, 9 Apr 2009 06:58:45 -0700 (PDT) > > > From: Rene J Buesa > > > Subject: Re: [Histonet] Negative IHC controls > > > To: histonet@lists.utsouthwestern.edu, Sheila Haas > > > > > > Message-ID: <292901.13223.qm@web65712.mail.ac4.yahoo.com> > > > Content-Type: text/plain; charset=iso-8859-1 > > > > > > I used to run a negative control per case or per block within any > given case when doing IHC, but not for reagents. > > > Ren? J. > > > > > > --- On Wed, 4/8/09, Sheila Haas wrote: > > > > > > From: Sheila Haas > > > Subject: [Histonet] Negative IHC controls > > > To: histonet@lists.utsouthwestern.edu > > > Date: Wednesday, April 8, 2009, 2:57 PM > > > > > > I have a question concerning ANP.22570 on the CAP checklist. Could > you all > > > tell me how you are handling > > > negative controls for IHC staining? The question actually states > (and I've > > > confirmed with CAP) that we should be running two types of > negative controls. > > > One for reagents and one for each antibody in a run. I'd like to know > > > what the practice is. This seems very costly and time consuming. > Thanks in > > > advance! > > > > > > Sheila Haas > > > Laboratory Supervisor > > > Micro Path Laboratories > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > > > > > > > ------------------------------ > > > > > > Message: 7 > > > Date: Thu, 9 Apr 2009 10:14:48 -0400 > > > From: "Blazek, Linda" > > > Subject: RE: [Histonet] Formula 83 users? > > > To: "'rjbuesa@yahoo.com'" , Histonet > > > , Jacqueline Farnsworth > > > > > > Message-ID: > > > > <5A2BD13465E061429D6455C8D6B40E3908778D156E@IBMB7Exchange.digestivespecialists.com> > > > > > > Content-Type: text/plain; charset="iso-8859-1" > > > > > > > > > With all due respect Ren?; I have been using Formula 83 with my > coverslipper with no problems at all though have a glass coverslipper > not a tape. > > > Linda > > > > > > > > > Linda Blazek HT (ASCP) > > > Manager/Supervisor > > > GI Pathology of Dayton > > > 7415 Brandt Pike > > > Huber Heights, OH 45424 > > > Phone: (937) 293-4424 ext 7118 > > > Email: lblazek@digestivespecialists.com > > > > > > > > > > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > > > Sent: Thursday, April 09, 2009 9:56 AM > > > To: Histonet; Jacqueline Farnsworth > > > Subject: Re: [Histonet] Formula 83 users? > > > > > > Jacqueline: > > > Formula 83 is a naphthenic hydrocarbon that cannot be used with > coverslippers, is recyclable and while some people have been using it > for more than 20 years, others find it irritant. > > > Ren? J. > > > > > > --- On Wed, 4/8/09, Jacqueline Farnsworth > wrote: > > > > > > From: Jacqueline Farnsworth > > > Subject: [Histonet] Formula 83 users? > > > To: "Histonet" > > > Date: Wednesday, April 8, 2009, 12:31 PM > > > > > > HI all. > > > I have searched the Histonet archives and found a lot of very > positive reviews > > > regarding Formula 83. I am wondering if anyone has encountered any > issues with > > > using Formula 83 on a stainer and then xylene on a tape > coverslipper. Are there > > > any miscibility issues? Are you soaking in xylene prior to tape > coverslipping > > > required? Any helpful hints/tips/tricks? > > > > > > Thank you in advance. > > > > > > > > > Jacqueline Farnsworth > > > Anatomic Pathology, Tech III > > > Foothills Medical Centre > > > Calgary Laboratory Services > > > > > > Ph: 403-944-1578 > > > Fax: 403-944-4748 > > > P Please consider the environment before printing this email. > > > > > > ________________________________ > > > This message and any attached documents are only for the use of > the intended > > > recipient(s), are confidential and may contain privileged > information. Any > > > unauthorized review, use, retransmission, or other disclosure is > strictly > > > prohibited. If you have received this message in error, please > notify the sender > > > immediately, and then delete the original message. Thank you. > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > ------------------------------ > > > > > > Message: 8 > > > Date: Thu, 09 Apr 2009 10:11:11 -0400 > > > From: Geoff McAuliffe > > > Subject: Re: [Histonet] Spyware from an Attachment: > > > To: u know > > > Cc: histonet@lists.utsouthwestern.edu > > > Message-ID: <49DE01FF.100@umdnj.edu> > > > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > > > > > Dear List: > > > > > > I strongly suggest that you NOT use any links offered by people > with no > > > name and no affiliation. Yes, there are good spyware detection > programs > > > available at no cost (Adaware for example) but I don't think this > is one > > > of them. There are at least 3 potential saboteurs on this list. > > > > > > Geoff > > > > > > u know wrote: > > > > > >> Dear Listers, > > >> > > >> If you ever opened any strange attachments from this list, you > should really consider scanning your Hard Disk for spyware. There is a > great free anti-spyware program we use, which you can get at > http://tinyurl.com/ynupj4 <----that is a virus-proof link, by the way. > > >> > > >> > > >> > > >> > > >> _______________________________________________ > > >> Histonet mailing list > > >> Histonet@lists.utsouthwestern.edu > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >> > > >> > > >> > > >> > > > > > > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------------------ > Rediscover Hotmail?: Now available on your iPhone or BlackBerry Check > it out. > From nw30 <@t> columbia.edu Thu Apr 16 09:15:13 2009 From: nw30 <@t> columbia.edu (Nan Wang) Date: Thu Apr 16 09:15:20 2009 Subject: [Histonet] email Message-ID: <49E73D71.8040600@columbia.edu> Hi, Can you remove me from the email list? Too many emails. Thanks. nan wang From JWeems <@t> sjha.org Thu Apr 16 09:29:20 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Apr 16 09:29:26 2009 Subject: [Histonet] container for undiluted bleach In-Reply-To: References: Message-ID: <5D64396A0D4A5346BEBC759022AAEAA5477F5C@ITSSSXM01V6.one.ads.che.org> I would use an empty Tilex bottle - with the labels stripped off. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, 16 April 2009 6:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] container for undiluted bleach Hello again We keep a small squirt bottle of undiluted bleach in our hood but it's constantly eating through the plastic. Any ideas on other ways to store about 150 ml in a squirt bottle or what type of plastic is bleach-proof? The point here is that we want to store a small amount in the hood and possibly be able to squirt instead of pour it. Emily prometheus, thief of light, giver of light, bound by the gods, must have been a book. -mark danielewski, house of leaves _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From jqb7 <@t> cdc.gov Thu Apr 16 09:29:53 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Apr 16 09:30:14 2009 Subject: [Histonet] email In-Reply-To: <49E73D71.8040600@columbia.edu> References: <49E73D71.8040600@columbia.edu> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE1BF05D6@LTA3VS011.ees.hhs.gov> You must go to the Histonet site and follow the instructions to remove yourself. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nan Wang Sent: Thursday, April 16, 2009 10:15 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] email Hi, Can you remove me from the email list? Too many emails. Thanks. nan wang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jane.Moose <@t> NewberryHospital.net Thu Apr 16 09:41:45 2009 From: Jane.Moose <@t> NewberryHospital.net (Jane C. Moose) Date: Thu Apr 16 09:47:22 2009 Subject: [Histonet] ISO Message-ID: <8BB5FC36DDA373488E60177757BBD69852C32C@ncmhexchbe01.NewberryHospital.net> Is anyone in histoland ISO certified or working on it. Our rural hospital for which the lab is CAP accredited has decided to become ISO certified, all departments. It is a 2 year plan and we have just started. It is quite a process to correlate CAP and ISO. We are not doing the CAP-- ISO process. Would appreciate any insight or suggestions. Thanks Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 From histonet.nospam <@t> vneubert.com Thu Apr 16 10:03:02 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Thu Apr 16 10:03:12 2009 Subject: [Histonet] email In-Reply-To: <49E73D71.8040600@columbia.edu> References: <49E73D71.8040600@columbia.edu> Message-ID: <49E748A6.1050806@vneubert.com> You can do that on your own - see http://lists.utsouthwestern.edu/mailman/listinfo/histonet Spam stopped - why not stay and share histo knowledge? :) Nan Wang wrote: > Hi, > Can you remove me from the email list? Too many emails. Thanks. > > nan wang > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Beth.Fye <@t> HCAhealthcare.com Thu Apr 16 10:30:23 2009 From: Beth.Fye <@t> HCAhealthcare.com (Fye Beth) Date: Thu Apr 16 10:30:36 2009 Subject: [Histonet] prostate biopsy kits Message-ID: <938F8EC5A524D34EB5796E23E52781D3043204B6F4@NADCWPMSGCMS05.hca.corpad.net> Hi Jodi, We get ours from STATLAB for under $5.00 a box. There is a pricing difference for the 8 vs. 12 bottle kit. It is almost identical to Bostwick's box. Considering the number of bx's that you are able to charge for the price is worth it, I think. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 From Lori.Disher <@t> HCAhealthcare.com Thu Apr 16 10:33:38 2009 From: Lori.Disher <@t> HCAhealthcare.com (Disher Lori) Date: Thu Apr 16 10:33:47 2009 Subject: [Histonet] Lab Week-Histology Trivial or Fun Facts Message-ID: <778DD853CF606049A37FC2059C8BA07A2ACBEADBAC@FWDCWPMSGCMS04.hca.corpad.net> Thank you all for you creative ideas and helpful web sites. I want to wish everyone a great National Medical Laboratory Professionals Week! From rjbuesa <@t> yahoo.com Thu Apr 16 10:34:34 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 16 10:34:39 2009 Subject: [Histonet] ISO In-Reply-To: <8BB5FC36DDA373488E60177757BBD69852C32C@ncmhexchbe01.NewberryHospital.net> Message-ID: <723850.38920.qm@web65709.mail.ac4.yahoo.com> Last?year CAP began issuing ISO certifications for the med lab (ISO15189-2003) which uses more statistical analysis and is adaptable to the med lab where most of the results are quantitative. CAP offered the certification as an additional one and not to substitute its own certification. As for the histolab, the only ISO that could be used is ISO9001-2000 that is just a management tool where the results are guaranteed to be obtained according to standards that are not general but particular of each lab, is like saying that the procedures follow what?is described in the SOP. Regarding the histolab productivity each one will have to define their own standards and assure that they are met. The CAP certification for histolabs is more than enough, but in the competitive health care?environment any good salesperson working for any given med lab can use an "ISO certification" as a sales enticement for the potential clients, even if they do not know what ISO is. Ren? J. --- On Thu, 4/16/09, Jane C. Moose wrote: From: Jane C. Moose Subject: [Histonet] ISO To: histonet@lists.utsouthwestern.edu Date: Thursday, April 16, 2009, 10:41 AM Is anyone in histoland ISO certified or working on it. Our rural hospital for which the lab is CAP accredited has decided to become ISO certified, all departments. It is a 2 year plan and we have just started. It is quite a process to correlate CAP and ISO. We are not doing the CAP-- ISO process. Would appreciate any insight or suggestions. Thanks Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu Apr 16 10:56:31 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Apr 16 10:56:41 2009 Subject: [Histonet] Acetone fixed tissue for PPFE Message-ID: Question: I have acetone fixed tissue (someone trying to duplicate a paper) for paraffin embedding? Should I go straight to absolute for processing? Thanks, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 From rjbuesa <@t> yahoo.com Thu Apr 16 12:02:12 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 16 12:02:16 2009 Subject: [Histonet] Acetone fixed tissue for PPFE In-Reply-To: Message-ID: <733951.28784.qm@web65712.mail.ac4.yahoo.com> Acetone acts as a dehydrating agent, so you do not need to go to ethanol now, but you will have to go to the ante medium (clearing agent) you use before the paraffin infiltration. It is possible that because the tissue were in acetone for a more than "conventional" period of time, the tissues have become brittle and you may have problems when sectioning. Ren? J. --- On Thu, 4/16/09, Bernice Frederick wrote: From: Bernice Frederick Subject: [Histonet] Acetone fixed tissue for PPFE To: histonet@lists.utsouthwestern.edu Date: Thursday, April 16, 2009, 11:56 AM Question: I have acetone fixed tissue (someone trying to duplicate a paper) for paraffin embedding? Should I go straight to absolute for processing? Thanks, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryeo <@t> wchosp.org Thu Apr 16 12:04:32 2009 From: ryeo <@t> wchosp.org (Richard Yeo) Date: Thu Apr 16 12:06:34 2009 Subject: [Histonet] training materials/policies/protocols Message-ID: Hey, Histonet Gurus My pathologist wants me to find any related materials/policies/protocols for training a histo-tech that qualifies through both CLIA/CAP to do grossing. I would greatly appreciate any help I can get as far as either sharing anything or where I can go to find it myself. Thanks Rich Y Richard E Yeo HT(ASCP) Histology Supervisor Wooster Community Hospital Wooster Ohio ryeo@wchosp.org ph-(330)263-8563 fx-(330)263-8582 PRIVILEGE AND CONFIDENTIALITY NOTICE The information in this electronic mail is intended for the named recipients only. It may contain privileged and confidential material and may be protected under law by the Health Insurance Portability and Accountability Act. Any use of this information by anyone other than the intended receiver is prohibited. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately by replying to this electronic e-mail. Please delete it from your computer. Thank you From jp1000r <@t> hotmail.com Thu Apr 16 12:19:59 2009 From: jp1000r <@t> hotmail.com (JP Rey) Date: Thu Apr 16 12:21:04 2009 Subject: [Histonet] Missouri Society for Histotechnology 2009 Symposium Message-ID: The Missouri Society for Histotechnology will have its annual symposium hold at the beautiful Lake of the Ozarks in a remarkable facility, Resort at Port Arrowhead from Thursday May 28th to Saturday May 30th, 2009. To obtain more information: * Check our website at: http://www.missouri-histo.org/index.cfm?p=4&pid=4274&spid=4295 * Or our video at: http://www.youtube.com/watch?v=6TgQAJ7J6UM We are looking forward meeting you there! Jean-Philippe REY MHT Vice President 4441 Gillham Road Kansas City, MO -64110 jp1000r@hotmail.com www.missouri-histo.org Tel.:(816) 213-2558 Tel.:(913) 945-6763 Fax.: (913) 660-0637 From dvigil000 <@t> centurytel.net Thu Apr 16 13:30:48 2009 From: dvigil000 <@t> centurytel.net (Debbie Vigil) Date: Thu Apr 16 13:27:41 2009 Subject: [Histonet] pH meter In-Reply-To: <200904151711.n3FHBL6o012128@ppsfilter4.tr.txstate.edu> References: <200904151711.n3FHBL6o012128@ppsfilter4.tr.txstate.edu> Message-ID: I am looking for a pH meter for Histology. We only need it for Hematoxylin, Eosin, and Water. I would appreciate any info on which probes to use, model, and protocols. Thanks, D Vigil From wilson_c <@t> ricerca.com Thu Apr 16 14:29:50 2009 From: wilson_c <@t> ricerca.com (Wilson, Carol) Date: Thu Apr 16 14:29:56 2009 Subject: [Histonet] Human tumors from mouse xenograft IHC HELP! Message-ID: <9D443EB9D0270143B5AAF190CB1A58A309746DF5@dogwood.ricerca.com> Can anybody out there provide protocols and/or vendors for the following antibodies for human tumors from mouse xenograft: ORC1L, MCM4, MCM6, MCM7, Cyclin D3, and FEN1? I am trying to show my boss that this is not as simple as he seems to think it is for a lab that is not basically set up to do even basic IHC. Thanks in advance... Carol Carol Wilson, HT(ASCP) Lead Technician/Histology From BMolinari <@t> heart.thi.tmc.edu Thu Apr 16 14:30:05 2009 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Apr 16 14:30:12 2009 Subject: [Histonet] glass Message-ID: I have some glass knife strips measuring 16"x1.5"x1/4" . We found them during some lab spring cleaning. They are free to whoever may want them. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 From CMCCOLLOUGH <@t> dnr.state.md.us Thu Apr 16 14:54:01 2009 From: CMCCOLLOUGH <@t> dnr.state.md.us (McCollough, Carol) Date: Thu Apr 16 14:54:08 2009 Subject: [Histonet] microtome maintenance training Message-ID: <236F0AA9C2DD9B43B16FAE14308FD9A33FA6A9@CENTMAIL.langroup.dnr.md> Hello everyone: I've been off Histonet for a few years, and am glad to be back. I have a tech who is interested in learning to service (PM) microtomes. Has there been an NSH or regional workshop on this topic recently? Can anyone suggest a vendor or other program, if not NSH? Many thanks! Regards - Carol B. McCollough Aquatic Animal Research Pathologist Oyster Disease Research Project Fisheries Service Maryland Department of Natural Resources Cooperative Oxford Laboratory 904 S. Morris Street Oxford, Maryland 21654 410-226-5193 The world is full of signals that we don't perceive." -- Stephen Jay Gould, (1941-2002) paleontologist, evolutionary biologist, science historian From lucie.s.guernsey <@t> gmail.com Thu Apr 16 17:07:26 2009 From: lucie.s.guernsey <@t> gmail.com (Lucie Guernsey) Date: Thu Apr 16 17:07:46 2009 Subject: [Histonet] Masson Trichrome Message-ID: Hi Histonetters - I could really use your help! I recently received a protocol for a Masson Trichrome stain that, if followed exactly, does not seem to work. I've been doing a lot of online and publication research, and yet I still have questions. I'm currently attempting to stain 3 um thick PFA-fixed paraffin kidney sections (mouse and rat). The following is my current protocol with my questions/problems in *bold*. Thank you so much in advance - I'm pulling my hair out over this! 1. Standard deparaffinization/rehydration. 2. Bouin's solution for 30 min at 56-60 degrees C 3. Running tap water for 8 min (or until not yellow). Rinse with dH2O. 4. Weigert's iron hematoxylin for *1 hr (all protocols claim 5 min - what am I doing wrong???? Do the stock solutions need to ripen before use????)* - Solution A: 5 g Hematoxylin + 500 mL 95% EtOH - Solution B: 20 mL 30% aqueous Ferric Chloride + 500 mL dH2O + 5 mL HCl, concentrated - WORK solution: equal parts Solution A and Solution B - made immediately before use - turns black 5. Running tap water for 5 min. Rinse with dH2O. 6. Scarlet Acid Fuchsin for 5 min. *(I realize that most protocols call for Biebrich Scarlet - are Biebrich or Xylidine Ponceau* *necessary????) * - 0.5 g Acid Fuchsin + 0.5 mL Acetic Acid, glacial + 100 mL dH2O 6. Rinse with dH2O. 7. 1% Phosphotungstic acid for 8 min *(most protocols call for a phosphotungstic/phosphomolybdic acid mixture - is PMA necessary????)* - 1 g Phosphotungstic Acid + 100 mL dH2O 8. Aniline blue - *the time for this is what I'm struggling to determine - have tried 5, 10, 15, 20, 25 min and all have been very light/pale - will try even longer times, though most protocols suggest 5-10 min....* - 2.5 g Aniline Blue + 2.5 mL Acetic Acid, glacial + 100 mL dH2O 9. Rinse with dH2O. 10. 1% Acetic acid for 1 min 30 sec *(I will try decreasing to 1 min in an attempt to get my aniline blue to stay darker)* 11. Rinse with dH2O. 12. Dehydrate: 95%, 100%, 100% EtOH, 30 sec. each. 13. Clear: Xylene, 3x, 3 min. each. 14. Mount using DPX (salicylic balsam based mounting medium) * While my hematoxylin works if I stain for an hour, I would love to know how people are able to stain for only 5 min - when I try that, it all just rinses out by the time I mount the slides.... * My scarlet acid works ok - light pink to reddish - but if I was to use Beibrich or Ponceau, would it make it better/clearer? * Is phosphomolybdic acid necessary for good differentiation? If so, does anyone have a quality, but inexpensive PMA that they use and can recommend? * How long for aniline blue/acetic acid? * I get quite a bit of purple - obviously a mix of red and blue - but is it too much red and too much blue, or is it that blue hasn't replaced all the red yet???? * How many times can you reuse the scarlet acid and aniline blue solutions? The hematoxylin, phosphotungstic acid, and acetic acid solutions are one-time use, correct? Thank you in advance for all your suggestions! Lucie Guernsey University of California, San Diego From AnthonyH <@t> chw.edu.au Thu Apr 16 18:06:07 2009 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Apr 16 18:06:16 2009 Subject: [Histonet] Masson Trichrome In-Reply-To: Message-ID: A few comments that might be of help: Allow the alcoholic haematoxylin to ripen for a few weeks prior to use or if in a hurry use a celestine blue-Harris's Haematoxylin stain. Use the smooth muscle cells in blood vessels to gauge the differentiation step. My experience is to differentiate until a little pink still remains in the collagen since the acidic blue or green solution will continue to differentiate the red muscle stain. The blue collagen solution stains faster than the green solutions so use for a shorter time. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Friday, 17 April 2009 8:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Masson Trichrome Hi Histonetters - I could really use your help! I recently received a protocol for a Masson Trichrome stain that, if followed exactly, does not seem to work. I've been doing a lot of online and publication research, and yet I still have questions. I'm currently attempting to stain 3 um thick PFA-fixed paraffin kidney sections (mouse and rat). The following is my current protocol with my questions/problems in *bold*. Thank you so much in advance - I'm pulling my hair out over this! 1. Standard deparaffinization/rehydration. 2. Bouin's solution for 30 min at 56-60 degrees C 3. Running tap water for 8 min (or until not yellow). Rinse with dH2O. 4. Weigert's iron hematoxylin for *1 hr (all protocols claim 5 min - what am I doing wrong???? Do the stock solutions need to ripen before use????)* - Solution A: 5 g Hematoxylin + 500 mL 95% EtOH - Solution B: 20 mL 30% aqueous Ferric Chloride + 500 mL dH2O + 5 mL HCl, concentrated - WORK solution: equal parts Solution A and Solution B - made immediately before use - turns black 5. Running tap water for 5 min. Rinse with dH2O. 6. Scarlet Acid Fuchsin for 5 min. *(I realize that most protocols call for Biebrich Scarlet - are Biebrich or Xylidine Ponceau* *necessary????) * - 0.5 g Acid Fuchsin + 0.5 mL Acetic Acid, glacial + 100 mL dH2O 6. Rinse with dH2O. 7. 1% Phosphotungstic acid for 8 min *(most protocols call for a phosphotungstic/phosphomolybdic acid mixture - is PMA necessary????)* - 1 g Phosphotungstic Acid + 100 mL dH2O 8. Aniline blue - *the time for this is what I'm struggling to determine - have tried 5, 10, 15, 20, 25 min and all have been very light/pale - will try even longer times, though most protocols suggest 5-10 min....* - 2.5 g Aniline Blue + 2.5 mL Acetic Acid, glacial + 100 mL dH2O 9. Rinse with dH2O. 10. 1% Acetic acid for 1 min 30 sec *(I will try decreasing to 1 min in an attempt to get my aniline blue to stay darker)* 11. Rinse with dH2O. 12. Dehydrate: 95%, 100%, 100% EtOH, 30 sec. each. 13. Clear: Xylene, 3x, 3 min. each. 14. Mount using DPX (salicylic balsam based mounting medium) * While my hematoxylin works if I stain for an hour, I would love to know how people are able to stain for only 5 min - when I try that, it all just rinses out by the time I mount the slides.... * My scarlet acid works ok - light pink to reddish - but if I was to use Beibrich or Ponceau, would it make it better/clearer? * Is phosphomolybdic acid necessary for good differentiation? If so, does anyone have a quality, but inexpensive PMA that they use and can recommend? * How long for aniline blue/acetic acid? * I get quite a bit of purple - obviously a mix of red and blue - but is it too much red and too much blue, or is it that blue hasn't replaced all the red yet???? * How many times can you reuse the scarlet acid and aniline blue solutions? The hematoxylin, phosphotungstic acid, and acetic acid solutions are one-time use, correct? Thank you in advance for all your suggestions! Lucie Guernsey University of California, San Diego _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From lpwenk <@t> sbcglobal.net Thu Apr 16 19:14:06 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Apr 16 19:14:16 2009 Subject: [Histonet] Masson Trichrome In-Reply-To: Message-ID: <63D609073E9A4425BD418C06F9F8B2D5@HPPav2> I'll send you our procedure under a separate cover. Couple of thoughts: 1. Try extending time in Bouins to 1 hour. If you put room temperature Bouins in the 60 degree oven with your slides, at 30 minutes, it's just finally getting up to 60 degrees. So extend the time to 45-60 minutes, so it has 15-30 minutes at 60 degrees. 2. Weigert's usually ends up being pulled out. Most of the time, the pathologists don't even notice that the nuclei are red. They just care to differentiate blue collagen from red muscle/cytoplasm. 3. There are a variety of red dyes that can be used, each one giving you a slightly different shade and intensity of red. Most are interchangeable - many of the Ponceau varieties can be used, as well as biebrich scarlet, acid fuchsin, etc. Of the Ponceau, the Ponceau S (CI #27195) seems to be most intense, but is a little more on the pinky/magenta side of red. 4. We use just PTA. PMA costs a lot more, and we didn't find any improvement over plain ole PTA. 5. The Aniline blue is probably the most important step, and works best if checked with the microscope. Some specimens become blue very quickly, and look best if pulled out after 1-2 minutes. Some need 5 minutes. Some need 10 minutes. I've found, it the collagen doesn't stain by 10 minutes, it's not going to get any bluer, and in fact the red muscle begins to pick up some of the blue after 10 minutes. I also found, the more you wash in water to check the intensity of blue, the more the red muscle picks up blue, and the blue collagen begins to look purplish. So I check it at 2 minutes and 5 minutes, and pull it out at 2 or 5, if it's the right intensity. If the blue is too pale at 5 minutes, I leave it for the full 10 minutes, and then pull it out at that point. 6. Cut down the time of water rinses after aniline blue to a couple of changes, 5 seconds each. (see #5 for reason). 7. If you are having problems with pale blue intensity, skip the acetic acid rinse completely. Make the stain more "delicate", but can be skipped. Peggy A. Wenk, HTL(ASCP)SL Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Thursday, April 16, 2009 6:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Masson Trichrome Hi Histonetters - I could really use your help! I recently received a protocol for a Masson Trichrome stain that, if followed exactly, does not seem to work. I've been doing a lot of online and publication research, and yet I still have questions. I'm currently attempting to stain 3 um thick PFA-fixed paraffin kidney sections (mouse and rat). The following is my current protocol with my questions/problems in *bold*. Thank you so much in advance - I'm pulling my hair out over this! 1. Standard deparaffinization/rehydration. 2. Bouin's solution for 30 min at 56-60 degrees C 3. Running tap water for 8 min (or until not yellow). Rinse with dH2O. 4. Weigert's iron hematoxylin for *1 hr (all protocols claim 5 min - what am I doing wrong???? Do the stock solutions need to ripen before use????)* - Solution A: 5 g Hematoxylin + 500 mL 95% EtOH - Solution B: 20 mL 30% aqueous Ferric Chloride + 500 mL dH2O + 5 mL HCl, concentrated - WORK solution: equal parts Solution A and Solution B - made immediately before use - turns black 5. Running tap water for 5 min. Rinse with dH2O. 6. Scarlet Acid Fuchsin for 5 min. *(I realize that most protocols call for Biebrich Scarlet - are Biebrich or Xylidine Ponceau* *necessary????) * - 0.5 g Acid Fuchsin + 0.5 mL Acetic Acid, glacial + 100 mL dH2O 6. Rinse with dH2O. 7. 1% Phosphotungstic acid for 8 min *(most protocols call for a phosphotungstic/phosphomolybdic acid mixture - is PMA necessary????)* - 1 g Phosphotungstic Acid + 100 mL dH2O 8. Aniline blue - *the time for this is what I'm struggling to determine - have tried 5, 10, 15, 20, 25 min and all have been very light/pale - will try even longer times, though most protocols suggest 5-10 min....* - 2.5 g Aniline Blue + 2.5 mL Acetic Acid, glacial + 100 mL dH2O 9. Rinse with dH2O. 10. 1% Acetic acid for 1 min 30 sec *(I will try decreasing to 1 min in an attempt to get my aniline blue to stay darker)* 11. Rinse with dH2O. 12. Dehydrate: 95%, 100%, 100% EtOH, 30 sec. each. 13. Clear: Xylene, 3x, 3 min. each. 14. Mount using DPX (salicylic balsam based mounting medium) * While my hematoxylin works if I stain for an hour, I would love to know how people are able to stain for only 5 min - when I try that, it all just rinses out by the time I mount the slides.... * My scarlet acid works ok - light pink to reddish - but if I was to use Beibrich or Ponceau, would it make it better/clearer? * Is phosphomolybdic acid necessary for good differentiation? If so, does anyone have a quality, but inexpensive PMA that they use and can recommend? * How long for aniline blue/acetic acid? * I get quite a bit of purple - obviously a mix of red and blue - but is it too much red and too much blue, or is it that blue hasn't replaced all the red yet???? * How many times can you reuse the scarlet acid and aniline blue solutions? The hematoxylin, phosphotungstic acid, and acetic acid solutions are one-time use, correct? Thank you in advance for all your suggestions! Lucie Guernsey University of California, San Diego _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Thu Apr 16 21:23:27 2009 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Apr 16 21:23:37 2009 Subject: [Histonet] Masson Trichrome In-Reply-To: References: Message-ID: Go to the StainsFile web site and read the pages on trichrome staining. http://stainsfile.info/StainsFile/theory/tri_gen.htm The subject is covered in detail. The site also has several variants of Masson's trichrome, both his original methods with the dye combinations he proposed and several modifications. Bryan Llewellyn ----- Original Message ----- From: "Lucie Guernsey" To: Sent: Thursday, April 16, 2009 3:07 PM Subject: [Histonet] Masson Trichrome > Hi Histonetters - I could really use your help! I recently received a > protocol for a Masson Trichrome stain that, if followed exactly, does not > seem to work. I've been doing a lot of online and publication research, > and > yet I still have questions. I'm currently attempting to stain 3 um thick > PFA-fixed paraffin kidney sections (mouse and rat). The following is my > current protocol with my questions/problems in *bold*. Thank you so much > in > advance - I'm pulling my hair out over this! > > 1. Standard deparaffinization/rehydration. > 2. Bouin's solution for 30 min at 56-60 degrees C > 3. Running tap water for 8 min (or until not yellow). Rinse with dH2O. > 4. Weigert's iron hematoxylin for *1 hr (all protocols claim 5 min - what > am > I doing wrong???? Do the stock solutions need to ripen before use????)* > - Solution A: 5 g Hematoxylin + 500 mL 95% EtOH > - Solution B: 20 mL 30% aqueous Ferric Chloride + 500 mL dH2O + 5 > mL HCl, concentrated > - WORK solution: equal parts Solution A and Solution B - made > immediately before use - turns black > 5. Running tap water for 5 min. Rinse with dH2O. > 6. Scarlet Acid Fuchsin for 5 min. *(I realize that most protocols call > for > Biebrich Scarlet - are Biebrich or Xylidine Ponceau* *necessary????) > * - 0.5 g Acid Fuchsin + 0.5 mL Acetic Acid, glacial + 100 mL dH2O > 6. Rinse with dH2O. > 7. 1% Phosphotungstic acid for 8 min *(most protocols call for a > phosphotungstic/phosphomolybdic acid mixture - is PMA necessary????)* > - 1 g Phosphotungstic Acid + 100 mL dH2O > 8. Aniline blue - *the time for this is what I'm struggling to determine - > have tried 5, 10, 15, 20, 25 min and all have been very light/pale - will > try even longer times, though most protocols suggest 5-10 min....* > - 2.5 g Aniline Blue + 2.5 mL Acetic Acid, glacial + 100 mL dH2O > 9. Rinse with dH2O. > 10. 1% Acetic acid for 1 min 30 sec *(I will try decreasing to 1 min in an > attempt to get my aniline blue to stay darker)* > 11. Rinse with dH2O. > 12. Dehydrate: 95%, 100%, 100% EtOH, 30 sec. each. > 13. Clear: Xylene, 3x, 3 min. each. > 14. Mount using DPX (salicylic balsam based mounting medium) > > > * While my hematoxylin works if I stain for an hour, I would love to know > how people are able to stain for only 5 min - when I try that, it all just > rinses out by the time I mount the slides.... > * My scarlet acid works ok - light pink to reddish - but if I was to use > Beibrich or Ponceau, would it make it better/clearer? > * Is phosphomolybdic acid necessary for good differentiation? If so, does > anyone have a quality, but inexpensive PMA that they use and can > recommend? > * How long for aniline blue/acetic acid? > * I get quite a bit of purple - obviously a mix of red and blue - but is > it > too much red and too much blue, or is it that blue hasn't replaced all the > red yet???? > * How many times can you reuse the scarlet acid and aniline blue > solutions? > The hematoxylin, phosphotungstic acid, and acetic acid solutions are > one-time use, correct? > > Thank you in advance for all your suggestions! > > Lucie Guernsey > University of California, San Diego > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Apr 17 07:39:48 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 17 07:39:52 2009 Subject: [Histonet] pH meter In-Reply-To: Message-ID: <839706.65647.qm@web65716.mail.ac4.yahoo.com> You really do not need?a pH meter for hematoxylin or eosin. Perhaps for water? Ren? J. --- On Thu, 4/16/09, Debbie Vigil wrote: From: Debbie Vigil Subject: [Histonet] pH meter To: histonet@lists.utsouthwestern.edu Date: Thursday, April 16, 2009, 2:30 PM I am looking for a pH meter for Histology. We only need it for Hematoxylin, Eosin, and Water. I would appreciate any info on which probes to use, model, and protocols. Thanks, D Vigil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Fri Apr 17 09:36:59 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Apr 17 09:37:05 2009 Subject: [Histonet] CNS Fixation Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E67FD@nmdamailsvr.nmda.ad.nmsu.edu> Yes, I'm back. I need the expertise of this group. I am trying to convince my three pathologists that fixation in alcoholic formalin is the best route for whole brain and spinal cord. This has been an uphill battle and in order to prevent sections from peeling off during staining, I am still pre-drying necropsy brain slides for an extra 20 minutes before putting on the auto-stainer (even this does not prevent all instances). I need some expert ammunition for my "battle" despite the fact that the SOP I've written requires it and it is rarely followed. If alcoholic formalin is not as good as some other method, I'm open to suggestion. Of course, convincing them that thinner sections would also help - but one step at a time! Thank you in advance! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From JWeems <@t> sjha.org Fri Apr 17 09:49:49 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Apr 17 09:50:14 2009 Subject: [Histonet] CNS Fixation In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E67FD@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E67FD@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA547810C@ITSSSXM01V6.one.ads.che.org> I have found that brain sections that are air-dried overnight - then dried in the oven rarely ever wash off. We fix in 10% NBF. This is human - not sure if animal would be different. Best, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, April 17, 2009 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CNS Fixation Yes, I'm back. I need the expertise of this group. I am trying to convince my three pathologists that fixation in alcoholic formalin is the best route for whole brain and spinal cord. This has been an uphill battle and in order to prevent sections from peeling off during staining, I am still pre-drying necropsy brain slides for an extra 20 minutes before putting on the auto-stainer (even this does not prevent all instances). I need some expert ammunition for my "battle" despite the fact that the SOP I've written requires it and it is rarely followed. If alcoholic formalin is not as good as some other method, I'm open to suggestion. Of course, convincing them that thinner sections would also help - but one step at a time! Thank you in advance! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From kgrobert <@t> rci.rutgers.edu Fri Apr 17 10:22:19 2009 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Fri Apr 17 10:09:06 2009 Subject: [Histonet] CNS Fixation In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E67FD@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E67FD@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <49E89EAB.50600@rci.rutgers.edu> Sara- Welcome back! We use 10% NBF also to fix our mouse & rat brains and spinal cords, and I use Ultrastick slides for sectioning (6 um sections are standard here, that's what my boss likes). I bake them in a 60 degrees C oven for 30 minutes-overnight (depending on my schedule). It's rare that I have tissue come off the Ultrastick slides during staining...but then I stain my slides manually since we don't have an autostainer yet. Good luck, Kathleen Roberts Principal Lab Technician Neurotoxicology Laboratories Dept of Pharmacology & Toxicology Ernest Mario School of Pharmacy Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854 Breeden, Sara wrote: >Yes, I'm back. I need the expertise of this group. > > > >I am trying to convince my three pathologists that fixation in alcoholic >formalin is the best route for whole brain and spinal cord. This has >been an uphill battle and in order to prevent sections from peeling off >during staining, I am still pre-drying necropsy brain slides for an >extra 20 minutes before putting on the auto-stainer (even this does not >prevent all instances). I need some expert ammunition for my "battle" >despite the fact that the SOP I've written requires it and it is rarely >followed. If alcoholic formalin is not as good as some other method, >I'm open to suggestion. Of course, convincing them that thinner sections >would also help - but one step at a time! Thank you in advance! > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 4700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rjbuesa <@t> yahoo.com Fri Apr 17 10:27:53 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 17 10:27:56 2009 Subject: [Histonet] CNS Fixation In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E67FD@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <223288.34506.qm@web65713.mail.ac4.yahoo.com> Sara: We always fixed human brains and CNS material in NBF and I really would not like to use a mixed fixative that has 2 different ways of?action (coagulation and cross-linking). The problem can be solved at the sections level (6-8 ?m) and? totally draining the water from underneath the section, followed by oven drying at 60?C for at least 30 minutes. Our sections seldom fell. Ren? J. --- On Fri, 4/17/09, Breeden, Sara wrote: From: Breeden, Sara Subject: [Histonet] CNS Fixation To: histonet@lists.utsouthwestern.edu Date: Friday, April 17, 2009, 10:36 AM Yes, I'm back. I need the expertise of this group. I am trying to convince my three pathologists that fixation in alcoholic formalin is the best route for whole brain and spinal cord. This has been an uphill battle and in order to prevent sections from peeling off during staining, I am still pre-drying necropsy brain slides for an extra 20 minutes before putting on the auto-stainer (even this does not prevent all instances). I need some expert ammunition for my "battle" despite the fact that the SOP I've written requires it and it is rarely followed. If alcoholic formalin is not as good as some other method, I'm open to suggestion. Of course, convincing them that thinner sections would also help - but one step at a time! Thank you in advance! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Fri Apr 17 10:59:44 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Apr 17 10:59:48 2009 Subject: [Histonet] CNS Fixation In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA547810C@ITSSSXM01V6.one.ads.che.org> References: <4D14F0FC9316DD41972D5F03C070908B017E67FD@nmdamailsvr.nmda.ad.nmsu.edu> <5D64396A0D4A5346BEBC759022AAEAA547810C@ITSSSXM01V6.one.ads.che.org> Message-ID: <706447.78462.qm@web1111.biz.mail.sk1.yahoo.com> Ditto Joyce. This works with animal brain too. PKP ________________________________ From: "Weems, Joyce" To: "Breeden, Sara" ; histonet@lists.utsouthwestern.edu Sent: Friday, April 17, 2009 9:49:49 AM Subject: RE: [Histonet] CNS Fixation I have found that brain sections that are air-dried overnight - then dried in the oven rarely ever wash off. We fix in 10% NBF. This is human - not sure if animal would be different. Best, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, April 17, 2009 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CNS Fixation Yes, I'm back.? I need the expertise of this group. I am trying to convince my three pathologists that fixation in alcoholic formalin is the best route for whole brain and spinal cord.? This has been an uphill battle and in order to prevent sections from peeling off during staining, I am still pre-drying necropsy brain slides for an extra 20 minutes before putting on the auto-stainer (even this does not prevent all instances). I need some expert ammunition for my "battle" despite the fact that the SOP I've written requires it and it is rarely followed.? If alcoholic formalin is not as good as some other method, I'm open to suggestion. Of course, convincing them that thinner sections would also help - but one step at a time!? Thank you in advance! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbaraaalbert <@t> yahoo.com Fri Apr 17 11:15:03 2009 From: barbaraaalbert <@t> yahoo.com (Barbara Albert) Date: Fri Apr 17 11:15:12 2009 Subject: [Histonet] CNS Fixation In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA547810C@ITSSSXM01V6.one.ads.che.org> References: <4D14F0FC9316DD41972D5F03C070908B017E67FD@nmdamailsvr.nmda.ad.nmsu.edu> <5D64396A0D4A5346BEBC759022AAEAA547810C@ITSSSXM01V6.one.ads.che.org> Message-ID: <417666.16473.qm@web63704.mail.re1.yahoo.com> We've found this also.? Even 30-60 minutes air drying helps alot with human brain.?We use regular slides and NBF. Barbara Albert UCSF Medical Center San Francisco ________________________________ From: "Weems, Joyce" To: "Breeden, Sara" ; histonet@lists.utsouthwestern.edu Sent: Friday, April 17, 2009 7:49:49 AM Subject: RE: [Histonet] CNS Fixation I have found that brain sections that are air-dried overnight - then dried in the oven rarely ever wash off. We fix in 10% NBF. This is human - not sure if animal would be different. Best, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, April 17, 2009 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CNS Fixation Yes, I'm back.? I need the expertise of this group. I am trying to convince my three pathologists that fixation in alcoholic formalin is the best route for whole brain and spinal cord.? This has been an uphill battle and in order to prevent sections from peeling off during staining, I am still pre-drying necropsy brain slides for an extra 20 minutes before putting on the auto-stainer (even this does not prevent all instances). I need some expert ammunition for my "battle" despite the fact that the SOP I've written requires it and it is rarely followed.? If alcoholic formalin is not as good as some other method, I'm open to suggestion. Of course, convincing them that thinner sections would also help - but one step at a time!? Thank you in advance! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM? 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Fri Apr 17 11:33:59 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Apr 17 11:34:40 2009 Subject: [Histonet] CNS Fixation In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E67FD@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E67FD@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <49E8AF77.4030600@umdnj.edu> Hi Sara: I, and it seems others, disagree with the idea that alcoholic formalin is the best fixative for CNS. Buffered formalin is the fix of choice for animal and human CNS. Geoff Breeden, Sara wrote: > Yes, I'm back. I need the expertise of this group. > > > > I am trying to convince my three pathologists that fixation in alcoholic > formalin is the best route for whole brain and spinal cord. This has > been an uphill battle and in order to prevent sections from peeling off > during staining, I am still pre-drying necropsy brain slides for an > extra 20 minutes before putting on the auto-stainer (even this does not > prevent all instances). I need some expert ammunition for my "battle" > despite the fact that the SOP I've written requires it and it is rarely > followed. If alcoholic formalin is not as good as some other method, > I'm open to suggestion. Of course, convincing them that thinner sections > would also help - but one step at a time! Thank you in advance! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From ryeo <@t> wchosp.org Fri Apr 17 12:02:58 2009 From: ryeo <@t> wchosp.org (Richard Yeo) Date: Fri Apr 17 12:05:03 2009 Subject: FW: [Histonet] training materials/policies/protocols Message-ID: I'm not sure if my prior post went through or not. If it did indeed go through please for give me for posting it again. I didn't get any responses and my pathologist is really breathing down my back for anything I can come up with so he can start to train some histotechs. Thanks again Rich Y -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Yeo Sent: Thursday, April 16, 2009 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] training materials/policies/protocols Hey, Histonet Gurus My pathologist wants me to find any related materials/policies/protocols for training a histo-tech that qualifies through both CLIA/CAP to do grossing. I would greatly appreciate any help I can get as far as either sharing anything or where I can go to find it myself. Thanks Rich Y Richard E Yeo HT(ASCP) Histology Supervisor Wooster Community Hospital Wooster Ohio ryeo@wchosp.org ph-(330)263-8563 fx-(330)263-8582 PRIVILEGE AND CONFIDENTIALITY NOTICE The information in this electronic mail is intended for the named recipients only. It may contain privileged and confidential material and may be protected under law by the Health Insurance Portability and Accountability Act. Any use of this information by anyone other than the intended receiver is prohibited. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately by replying to this electronic e-mail. Please delete it from your computer. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGE AND CONFIDENTIALITY NOTICE The information in this electronic mail is intended for the named recipients only. It may contain privileged and confidential material and may be protected under law by the Health Insurance Portability and Accountability Act. Any use of this information by anyone other than the intended receiver is prohibited. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately by replying to this electronic e-mail. Please delete it from your computer. Thank you From pruegg <@t> ihctech.net Fri Apr 17 12:10:41 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Apr 17 12:10:48 2009 Subject: [Histonet] training materials/policies/protocols In-Reply-To: References: Message-ID: <6334AC72DB40429384AC85A6B4F1B40F@prueggihctechlt> Rich, There have been workshops at National (NSH), Regional and State meetings on grossing, but I do not know of any training programs per se. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Yeo Sent: Friday, April 17, 2009 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] training materials/policies/protocols I'm not sure if my prior post went through or not. If it did indeed go through please for give me for posting it again. I didn't get any responses and my pathologist is really breathing down my back for anything I can come up with so he can start to train some histotechs. Thanks again Rich Y -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Yeo Sent: Thursday, April 16, 2009 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] training materials/policies/protocols Hey, Histonet Gurus My pathologist wants me to find any related materials/policies/protocols for training a histo-tech that qualifies through both CLIA/CAP to do grossing. I would greatly appreciate any help I can get as far as either sharing anything or where I can go to find it myself. Thanks Rich Y Richard E Yeo HT(ASCP) Histology Supervisor Wooster Community Hospital Wooster Ohio ryeo@wchosp.org ph-(330)263-8563 fx-(330)263-8582 PRIVILEGE AND CONFIDENTIALITY NOTICE The information in this electronic mail is intended for the named recipients only. It may contain privileged and confidential material and may be protected under law by the Health Insurance Portability and Accountability Act. Any use of this information by anyone other than the intended receiver is prohibited. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately by replying to this electronic e-mail. Please delete it from your computer. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGE AND CONFIDENTIALITY NOTICE The information in this electronic mail is intended for the named recipients only. It may contain privileged and confidential material and may be protected under law by the Health Insurance Portability and Accountability Act. Any use of this information by anyone other than the intended receiver is prohibited. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately by replying to this electronic e-mail. Please delete it from your computer. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Fri Apr 17 12:10:48 2009 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Apr 17 12:10:54 2009 Subject: [Histonet] CNS Fixation References: <4D14F0FC9316DD41972D5F03C070908B017E67FD@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1F46C3E396F24598817EF6D54DB2AB16@auxs.umn.edu> Sara, This is what we do here with animal brain tissue: 1) Brain should fix for a 24-48 hrs in NBF. 2) They are trimmed in to about 5 mm thick (for embedding cassette). 3) Place cassettes face UP on the ice tray before sectioning (not face down). 3) The sections are cut at 4 um. 4) Waterbath temp is between 38-40. If they are exploding on the waterbath, then turn it down to about 34-36, making sure the wrinkles flatten out before sections are picked up. 5) Use adhesive slides. 6) For routine stains (H&E), the slides can either go in a 56C oven for 15 minutes to get the water out or go directly onto the autostainer. For IHC, they go into the oven at 80C for 30 minutes, then proceed with the IHC staining protocols. Brains usually don't stay on if they are not fixed long enough, processed completely or have to much moisture in them from the ice tray.So, we don't put the brains face down on the ice tray. They are face up on the ice tray. This way they don't soak up water, they just get chilled. Jan Shivers Section Head HIstology/IHC/EM Univ. of Minnesota Veterinary Diagnostic Lab St. Paul, MN ----- Original Message ----- From: "Breeden, Sara" To: Sent: Friday, April 17, 2009 9:36 AM Subject: [Histonet] CNS Fixation Yes, I'm back. I need the expertise of this group. I am trying to convince my three pathologists that fixation in alcoholic formalin is the best route for whole brain and spinal cord. This has been an uphill battle and in order to prevent sections from peeling off during staining, I am still pre-drying necropsy brain slides for an extra 20 minutes before putting on the auto-stainer (even this does not prevent all instances). I need some expert ammunition for my "battle" despite the fact that the SOP I've written requires it and it is rarely followed. If alcoholic formalin is not as good as some other method, I'm open to suggestion. Of course, convincing them that thinner sections would also help - but one step at a time! Thank you in advance! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Apr 17 12:23:59 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Apr 17 12:24:05 2009 Subject: [Histonet] aggrecan Message-ID: <4C43E13EE5314723AC25DF1A1CAB541C@prueggihctechlt> Does anyone on Histonet use aggrecan ab to label cartilage cells? I have an ab from Abcam ?ab3778 which is a mouse monoclonal, but they do not give any dilution recommendations for ffpe tissue IHC or pretreatments. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org From pruegg <@t> ihctech.net Fri Apr 17 12:26:36 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Apr 17 12:26:41 2009 Subject: [Histonet] brdu on ffpe rat fixed for up to 2 years in NBF Message-ID: Anybody tried doing IHC for brdu on ffpe rat intestine and skin fixed for up to 2 years in NBF? Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org From Barbara.Stancel <@t> fsis.usda.gov Fri Apr 17 12:29:02 2009 From: Barbara.Stancel <@t> fsis.usda.gov (Stancel, Barbara) Date: Fri Apr 17 12:29:09 2009 Subject: [Histonet] RE: ISO for Pathology In-Reply-To: <65F9BA4B48O568200-01@tweed3east> References: <65F9BA4B48O568200-01@tweed3east> Message-ID: Our laboratory is ISO 17025 certified/accredited. Having said that: Pathology is not under "scope:" our Chemistry and Microbiology sections are under "scope." Our certifying (external auditing)agency is AALA (American Association for Laboratory Accreditation). They have been auditing Chem and Micro for about 10 years. AALA (aka: A2LA) keep saying they will be covering the histology/pathology when they get people trained to perform audits. We have two levels of internal auditing: Laboratory Quality Assurance Division (LQAD) and our Quality Managers (QM). Again, we have a chem QM and a micro QM. Since our section is so small (9 people of the 90 folks) the Micro QM handles Pathology. I am getting ready for our spring internal audit. Pathology is expected to jump through ALL of the same hoops as chem and micro. I must say we have work instructions, tracking, and recording forms for everything except bathroom breaks and I am expecting them soon. My e-mail address is below. But if I am not in the lab I cannot access our work e-mail. So be patient with my responses. Histologically yours, Barbara Stancel USDA, FSIS, EL, Pathology Athens, Georgia 30605 706-546-3698 or 706-546-3556 barbara.stancel@fsis.usda.gov Subject: [Histonet] ISO Is anyone in histoland ISO certified or working on it. Our rural hospital for which the lab is CAP accredited has decided to become ISO certified, all departments. It is a 2 year plan and we have just started. It is quite a process to correlate CAP and ISO. We are not doing the CAP-- ISO process. Would appreciate any insight or suggestions. Thanks Jane Moose Jane.Moose@NewberryHospital.net LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 Subject: Re: [Histonet] ISO Last year CAP began issuing ISO certifications for the med lab (ISO15189-2003) which uses more statistical analysis and is adaptable to the med lab where most of the results are quantitative. CAP offered the certification as an additional one and not to substitute its own certification. As for the histolab, the only ISO that could be used is ISO9001-2000 that is just a management tool where the results are guaranteed to be obtained according to standards that are not general but particular of each lab, is like saying that the procedures follow what is described in the SOP. Regarding the histolab productivity each one will have to define their own standards and assure that they are met. The CAP certification for histolabs is more than enough, but in the competitive health care environment any good salesperson working for any given med lab can use an "ISO certification" as a sales enticement for the potential clients, even if they do not know what ISO is. Ren? J. Rene J Buesa From Jennifer.Bull <@t> northwestpathology.com Fri Apr 17 12:56:54 2009 From: Jennifer.Bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Fri Apr 17 12:57:00 2009 Subject: [Histonet] Question about floating sections off slides Message-ID: <85760CECEC18444BB95F26D5E88DAEAA223F261C06@hinet2.hinet.org> Does anyone have any experience in taking a stained H&E slide (non-charged), working backwards to rehydration, and floating the individual tissue sections off into a waterbath to be picked up on a charged slide? I can't come up with any possible way to successfully do this, but one of our Pathologists says he has heard of it being done and wants us to do it. Any advice will be much appreciated. Jennifer Bull Histology Supervisor Northwest Pathology jennifer.bull@nwpathology.com From victor <@t> pathology.washington.edu Fri Apr 17 13:04:26 2009 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Apr 17 13:04:31 2009 Subject: [Histonet] Question about floating sections off slides In-Reply-To: <85760CECEC18444BB95F26D5E88DAEAA223F261C06@hinet2.hinet.org> References: <85760CECEC18444BB95F26D5E88DAEAA223F261C06@hinet2.hinet.org> Message-ID: <49E8C4AA.6080200@pathology.washington.edu> Jennitfer, Found this in the archives from "Joe The Toe". I remember the procedure. 1. Take off the cover slip and soak in xylene or substitute for 15 minutes. 2. Apply enough Mount Quick to cover sections and place in an 80 degree oven for 30 minutes or until hardened. 3. place slide in tissue floatation bath for 1 hour or until the section can be lifted off with forceps. 4. wet slide(s) that will receive the transferred section with water. 5 remove the section carefully. If the section does not come off easy, keep in waterbath longer. This is important--- the side that was on the old slide must be placed on the slide exactly how it was removed because this will have a flat surface. 6. dry in oven again for 1 hour 7. clear, dehydrate and use as needed. One problem I encountered was with plus slides. I had to leave the slide in the floatation bath overnight to get better results, otherwise, only parts of the tissue would transfer. JTT Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Bull, Jennifer L. wrote: > Does anyone have any experience in taking a stained H&E slide (non-charged), working backwards to rehydration, and floating the individual tissue sections off into a waterbath to be picked up on a charged slide? I can't come up with any possible way to successfully do this, but one of our Pathologists says he has heard of it being done and wants us to do it. Any advice will be much appreciated. > > > Jennifer Bull > Histology Supervisor > Northwest Pathology > jennifer.bull@nwpathology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Montina.VanMeter <@t> pbrc.edu Fri Apr 17 13:39:07 2009 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Fri Apr 17 13:39:15 2009 Subject: [Histonet] 2009 Louisiana Society for Histotechnology Symposium/Convention Message-ID: <4FE7FB862E90E448AE32388E759220E5012CD577@pbrcas31.pbrc.edu> Hello Histonetters, The Louisiana Society for Histotechnology is pleased to announce the 26th Annual Symposium/Convention: "Your Histeaux Surplus Package" June 12 & 13, 2009 at the Bourbon Orleans Hotel 717 Orleans St. New Orleans, LA 70117 www.bourbonorleans.com The LSH block of rooms will be held until May 11, 2009. For those attendees who may want to visit the beautiful sights and sounds of New Orleans, the Bourbon Orleans Hotel will honor the room rates for three days prior and three days after the meeting. Special on-site parking rates for LSH attendees are available as well as public parking around Jackson Square. For reservations call 1-504-523-2222 or 1-866-513-9744 and mention you are with the LSH group. Do to relocation of many of our members we would ask everyone who would like to receive a brochure/membership form in the mail, email or fax, to please contact Tina Van Meter at 225-603-0953, vanmetmj@pbrc.edu , or Dixie Benoit at 337-233-1951, dixiehistochick@bellsouth.net. Walk-ins are always welcome, but pre-registrants will receive a complimentary buffet lunch each day! Please make additional copies of your registration form for coworkers in your lab or facility that might be interested in attending. The LSH would also like to extend the invitation to our fellow technologists and pathologists from surrounding states. We encourage everyone to attend in order to build our networking potential, earn those valuable CEU's and enjoy the beautiful city of New Orleans. We have a variety of topics presented by experienced speakers that promises to benefit everyone. The attendees will have access to several scientific vendor exhibits during the entire symposium. I have listed the workshops below and encourage y'all to come on down to the Bourbon Orleans Hotel in the French Quarter! 2009 LSH State Meeting Workshops: WS#1 - Am I Really Ready for this CAP Inspection? WS#2 - Mouse to Horse: Differences in working with human and animal tissue WS#3 - Breast Cancer and the Standardization of HER2/neu Testing WS#4 - Contemporary Trends in Immunohistochemistry WS#5 - Troubleshooting Routine Special Stains WS#6 - Which Code Do I Use? (CPT Coding) WS#7 - Are You REALLY Ready for the Next Catastrophe That Will Affect Your Hospital or City? WS#8 - 2 Crazy Cat Ladies Give Their Opinions of Life, Liberty, and Lab Management Thank you, Tina Van Meter Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 (Lab) From lucie.s.guernsey <@t> gmail.com Fri Apr 17 13:47:44 2009 From: lucie.s.guernsey <@t> gmail.com (Lucie Guernsey) Date: Fri Apr 17 13:48:03 2009 Subject: [Histonet] Re: Masson Trichrome In-Reply-To: References: Message-ID: Thank you all for your suggestions! Though, if anyone still has ideas, please don't consider this thank you as a closing of the subject - I (and I'm sure, others who are in my situation) would love to hear any and all variations that work. Now I'm off to cut more tissue so I can run more stains using your suggestions and, cross your fingers for me, hopefully (finally) optimize this stain for my lab.... Thanks again! Lucie Guernsey University of California, San Diego On Thu, Apr 16, 2009 at 3:07 PM, Lucie Guernsey wrote: > Hi Histonetters - I could really use your help! I recently received a > protocol for a Masson Trichrome stain that, if followed exactly, does not > seem to work. I've been doing a lot of online and publication research, and > yet I still have questions. I'm currently attempting to stain 3 um thick > PFA-fixed paraffin kidney sections (mouse and rat). The following is my > current protocol with my questions/problems in *bold*. Thank you so much > in advance - I'm pulling my hair out over this! > > 1. Standard deparaffinization/rehydration. > 2. Bouin's solution for 30 min at 56-60 degrees C > 3. Running tap water for 8 min (or until not yellow). Rinse with dH2O. > 4. Weigert's iron hematoxylin for *1 hr (all protocols claim 5 min - what > am I doing wrong???? Do the stock solutions need to ripen before use????)* > - Solution A: 5 g Hematoxylin + 500 mL 95% EtOH > - Solution B: 20 mL 30% aqueous Ferric Chloride + 500 mL dH2O + 5 > mL HCl, concentrated > - WORK solution: equal parts Solution A and Solution B - made > immediately before use - turns black > 5. Running tap water for 5 min. Rinse with dH2O. > 6. Scarlet Acid Fuchsin for 5 min. *(I realize that most protocols call > for Biebrich Scarlet - are Biebrich or Xylidine Ponceau* *necessary????) > * - 0.5 g Acid Fuchsin + 0.5 mL Acetic Acid, glacial + 100 mL dH2O > 6. Rinse with dH2O. > 7. 1% Phosphotungstic acid for 8 min *(most protocols call for a > phosphotungstic/phosphomolybdic acid mixture - is PMA necessary????)* > - 1 g Phosphotungstic Acid + 100 mL dH2O > 8. Aniline blue - *the time for this is what I'm struggling to determine - > have tried 5, 10, 15, 20, 25 min and all have been very light/pale - will > try even longer times, though most protocols suggest 5-10 min....* > - 2.5 g Aniline Blue + 2.5 mL Acetic Acid, glacial + 100 mL dH2O > 9. Rinse with dH2O. > 10. 1% Acetic acid for 1 min 30 sec *(I will try decreasing to 1 min in an > attempt to get my aniline blue to stay darker)* > 11. Rinse with dH2O. > 12. Dehydrate: 95%, 100%, 100% EtOH, 30 sec. each. > 13. Clear: Xylene, 3x, 3 min. each. > 14. Mount using DPX (salicylic balsam based mounting medium) > > > * While my hematoxylin works if I stain for an hour, I would love to know > how people are able to stain for only 5 min - when I try that, it all just > rinses out by the time I mount the slides.... > * My scarlet acid works ok - light pink to reddish - but if I was to use > Beibrich or Ponceau, would it make it better/clearer? > * Is phosphomolybdic acid necessary for good differentiation? If so, does > anyone have a quality, but inexpensive PMA that they use and can recommend? > * How long for aniline blue/acetic acid? > * I get quite a bit of purple - obviously a mix of red and blue - but is it > too much red and too much blue, or is it that blue hasn't replaced all the > red yet???? > * How many times can you reuse the scarlet acid and aniline blue solutions? > The hematoxylin, phosphotungstic acid, and acetic acid solutions are > one-time use, correct? > > Thank you in advance for all your suggestions! > > Lucie Guernsey > University of California, San Diego > From vapatpxs <@t> yahoo.com Fri Apr 17 15:11:18 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Fri Apr 17 15:11:23 2009 Subject: [Histonet] Biowave DFR-10 owner's manual Message-ID: <374878.67140.qm@web46110.mail.sp1.yahoo.com> Hello All, I have inherited a Biowave DFR-10 (a fancy Ted Pella microwave) but I did not inherit the owner's manual. Does anyone have a copy that they can send me? Thanks and have a nice weekend. Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 From baza0013 <@t> umn.edu Fri Apr 17 16:12:29 2009 From: baza0013 <@t> umn.edu (Adam Bazama) Date: Fri Apr 17 16:12:36 2009 Subject: [Histonet] HIF-1alpha or Ref-1 immunohistochemistry on mouse FFPE tissues Message-ID: <001201c9bfa1$37b098d0$a711ca70$@edu> Does anyone have any suggestions or a working immunofluorescence protocol for staining mouse FFPE tissues with HIF-1alpha rabbit polyclonal or Ref-1 rabbit polyclonal? Both from Santa Cruz. Thank you, Adam Adam Bazama baza0013@umn.edu Lillehei Heart Institute Histology Microscopy Research Core University of Minnesota 4-266 Nils Hasselmo Hall Minneapolis, MN 55455 612-625-6779 From Steven.Joy <@t> capitalhealth.ca Fri Apr 17 16:10:31 2009 From: Steven.Joy <@t> capitalhealth.ca (Steven Joy) Date: Fri Apr 17 16:14:32 2009 Subject: [Histonet] Gross Photography Message-ID: <1101A09566B25D4BB3889A44FDC1E7040A9B08@uantexchg03.capitalhealth.ca> Is there a range of practice among centers as to what specimens are photographed at gross, does anyone photograph pretty much all specimens? Steve Joy, BSc. MLT Research and Development Technologist 5B2.03 Anatomical Pathology University of Alberta Hospital 8440-112 st Edmonton Ab T6G 2B7 Phone: (780) 407-8015 Fax: (780) 407-3009 Email: steven.joy@capitalhealth.ca This communication is intended for the sole use of the recipient to which it was addressed and may contain confidential, personal or privileged information. Please contact the sender immediately if you are not the intended recipient of this information and do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. Thank you. From mpence <@t> grhs.net Fri Apr 17 16:20:39 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Apr 17 16:20:44 2009 Subject: [Histonet] Gross Photography In-Reply-To: <1101A09566B25D4BB3889A44FDC1E7040A9B08@uantexchg03.capitalhealth.ca> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3B15@IS-E2K3.grhs.net> I only photograph specimens that are not "routine" type specimens. Something that you might see only a few times a year or that once in a lifetime specimen. We also will get request from the surgeon to photograph a specimen for them at gross. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Joy Sent: Friday, April 17, 2009 4:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gross Photography Is there a range of practice among centers as to what specimens are photographed at gross, does anyone photograph pretty much all specimens? Steve Joy, BSc. MLT Research and Development Technologist 5B2.03 Anatomical Pathology University of Alberta Hospital 8440-112 st Edmonton Ab T6G 2B7 Phone: (780) 407-8015 Fax: (780) 407-3009 Email: steven.joy@capitalhealth.ca This communication is intended for the sole use of the recipient to which it was addressed and may contain confidential, personal or privileged information. Please contact the sender immediately if you are not the intended recipient of this information and do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bridget.maryott <@t> ventana.roche.com Fri Apr 17 16:38:57 2009 From: bridget.maryott <@t> ventana.roche.com (Maryott, Bridget) Date: Fri Apr 17 16:39:05 2009 Subject: [Histonet] microtome maintenance training Message-ID: <8642F0E8460EA64BB18056A8958CBC50020B5D34@dtumsem1.nala.roche.com> I have a tech who is interested in learning to service (PM) microtomes. Has there been an NSH or regional workshop on this topic recently? Can anyone suggest a vendor or other program, if not NSH? First off let me preface this noting that this is my first ever reply, so hopefully I am doing this correctly. Prior to being in the field of Histology my father and I had a small company doing Microscope/Microtome Service and repairs. Lucky for me Leica sent my dad to school to learn how to do microtome service. I in turn learned from him. I too looked into finding a program to become certified and found that none existed. Unless you work for an authorized microtome manufacturer nobody is interested in training you or selling you parts for repairs. Also the micrometers required to properly calibrate the instrument are VERY pricey and vary from instrument to instrument. Also the procedure for calibrating the instrument depends on the make and model. I think you may see where i'm heading with this. So I guess my answer is no, but while we are on the topic I can provide a few hints to making sure the microtome service you pay for is being done correct. 1) Be there when they calibrate the instrument. 2) Make sure they take the case off and use a micrometer while testing the section thickness. 3) Ask for a calibration report, including what thickness your instrument is cutting at, the allowed tolerance for that instrument, and if any adjustments were made. All to many times I have come upon service companies that have been charging customers for calibration when the case had never even been taken off instrument. The more you know the better. I know that's not the answer you were looking for but I hope it helps. Bridget Maryott Advanced Staining Ventana Medical Systems, Inc. a member of the Roche Group 1910 E. Innovation Park Drive Tucson, Arizona 85755 Tel: +1 520 229 4022 mailto: bridget.maryott@ventana.roche.com From tfountain <@t> exchange.hsc.mb.ca Fri Apr 17 17:26:10 2009 From: tfountain <@t> exchange.hsc.mb.ca (Tiana Fountain) Date: Fri Apr 17 17:35:56 2009 Subject: [Histonet] New Antibodies? Message-ID: Hello, I am looking for a recommendation for a new CD15 .. our current clone is LeuM1 and I would like to know the preferred one out there ... also I am looking at working up a Troma-1 and I was curious if anyone is doing it the with DAKO Envision plus system?? Anyone?? Thanks! Tiana -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From ccrowder <@t> vetmed.lsu.edu Fri Apr 17 17:57:03 2009 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Fri Apr 17 17:57:23 2009 Subject: [Histonet] 2009 Louisiana Society for Histotechnology 26th Annual Symposium/Convention Message-ID: Hello Histonetters, The Louisiana Society for Histotechnology is pleased to announce the 26th Annual Symposium/Convention: "Your Histeaux Surplus Package" June 12 & 13, 2009 at the Bourbon Orleans Hotel 717 Orleans St. New Orleans, LA 70117 www.bourbonorleans.com The LSH block of rooms will be held until May 11, 2009. For those attendees who may want to visit the beautiful sights and sounds of New Orleans, the Bourbon Orleans Hotel will honor the room rates for three days prior and three days after the meeting. Special on-site parking rates for LSH attendees are available as well as public parking around Jackson Square. For reservations call 1-504-523-2222 or 1-866-513-9744 and mention you are with the LSH group. Do to relocation of many of our members we would ask everyone who would like to receive a brochure/membership form in the mail, email or fax, to please contact Tina Van Meter at 225-603-0953, vanmetmj@pbrc.edu, or Dixie Benoit at 337-233-1951. Walk-ins are always welcome, but pre-registrants will receive a complimentary buffet lunch each day! Please make additional copies of your registration form for coworkers in your lab or facility that might be interested in attending. The LSH would also like to extend the invitation to our fellow technologists and pathologists from surrounding states. We encourage everyone to attend in order to build our networking potential, earn those valuable CEU's and enjoy the beautiful city of New Orleans. We have a variety of topics presented by experienced speakers that promises to benefit everyone. The attendees will have access to several scientific vendor exhibits during the entire symposium. I have listed the workshops below and encourage y?all to come on down to the Bourbon Orleans Hotel in the French Quarter! 2009 LSH State Meeting Workshops: WS#1 - Am I Really Ready for this CAP Inspection? WS#2 - Mouse to Horse: Differences in working with human and animal tissue WS#3 - Breast Cancer and the Standardization of HER2/neu Testing WS#4 - Contemporary Trends in Immunohistochemistry WS#5 - Troubleshooting Routine Special Stains WS#6 - Which Code Do I Use? WS#7 - Are You REALLY Ready for the Next Catastrophe That Will Affect Your Hospital or City? WS#8 - 2 Crazy Cat Ladies Give Their Opinions of Life, Liberty, and Lab Management Thank you, Tina Van Meter Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 From jnocito <@t> satx.rr.com Fri Apr 17 18:30:09 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Apr 17 18:30:16 2009 Subject: [Histonet] Gross Photography In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3B15@IS-E2K3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C017A3B15@IS-E2K3.grhs.net> Message-ID: <8948BE6AB7D44DB0914DD21F818B10BE@JoePC> like Mike, we only photograph unusual specimens. Seems photographing specimens has become less and less important. Kind of like autopsies. JTT ----- Original Message ----- From: "Mike Pence" To: "Steven Joy" ; Sent: Friday, April 17, 2009 4:20 PM Subject: RE: [Histonet] Gross Photography I only photograph specimens that are not "routine" type specimens. Something that you might see only a few times a year or that once in a lifetime specimen. We also will get request from the surgeon to photograph a specimen for them at gross. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Joy Sent: Friday, April 17, 2009 4:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gross Photography Is there a range of practice among centers as to what specimens are photographed at gross, does anyone photograph pretty much all specimens? Steve Joy, BSc. MLT Research and Development Technologist 5B2.03 Anatomical Pathology University of Alberta Hospital 8440-112 st Edmonton Ab T6G 2B7 Phone: (780) 407-8015 Fax: (780) 407-3009 Email: steven.joy@capitalhealth.ca This communication is intended for the sole use of the recipient to which it was addressed and may contain confidential, personal or privileged information. Please contact the sender immediately if you are not the intended recipient of this information and do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dermpathsy <@t> gmail.com Sat Apr 18 00:37:24 2009 From: dermpathsy <@t> gmail.com (Sate Hamza) Date: Sat Apr 18 00:37:42 2009 Subject: [Histonet] Gross Photography In-Reply-To: <8948BE6AB7D44DB0914DD21F818B10BE@JoePC> References: <661949901A768E4F9CC16D8AF8F2838C017A3B15@IS-E2K3.grhs.net> <8948BE6AB7D44DB0914DD21F818B10BE@JoePC> Message-ID: <8854ff80904172237t67076079xd8faf2323700ff35@mail.gmail.com> As a pathologist, I am a strong proponent of ample gross photography in the cutting room. When I first started in my current place, I thought that not much gross photography was being done. This has increased in recent years in our center. I always encourage our residents to take digital gross photographs. I recently bought an easy to use digital camera and gave it as a gift to our cutting room to encourage more digital photographs. I think that the availability of easy to use digital cameras has made taking pictures much easier. A picture is a great tool for documentation and for communication. No matter how skillful and expressive the gross description is, a picture can make things much easier for sign out. If sections need to be mapped for margins or other considerations, one can take a digital picture, make a quick print out of it and map the sections on it. Such pictures are so helpful, for example, for excisions of flat pigmented lesions from sun-damaged skin. Gross-microscopic correlation can help so much in assessing margin status (this can be so difficult with microscopy alone). It also helps in excisions of vulvar lesions/tumors and in irregularly shaped complex excisions from any site. The digital photos can be taken quickly. They do not need to be textbook quality. The goal usually is to facilitate communication and facilitate the sign-out process. The pictures that our PAs take are placed on a network server in folders that are named with the accession number. The printouts with sections mapped are kept in a binder in the cutting room where a pathologist can find them when the need arises. Sate On Fri, Apr 17, 2009 at 6:30 PM, Joe Nocito wrote: > like Mike, > we only photograph unusual specimens. Seems photographing specimens has > become less and less important. Kind of like autopsies. > -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada From kemlo <@t> f2s.com Sat Apr 18 03:00:45 2009 From: kemlo <@t> f2s.com (kemlo) Date: Sat Apr 18 03:01:11 2009 Subject: [Histonet] Gross Photography In-Reply-To: <8854ff80904172237t67076079xd8faf2323700ff35@mail.gmail.com> References: <661949901A768E4F9CC16D8AF8F2838C017A3B15@IS-E2K3.grhs.net> <8948BE6AB7D44DB0914DD21F818B10BE@JoePC> <8854ff80904172237t67076079xd8faf2323700ff35@mail.gmail.com> Message-ID: As a Biomedical Scientist I agree with you totally. One of the weaknesses of Biomedical scientists performing the 'grossing' is that the original evidence at dissection is lost to the Pathologist (that is until that Time Biomedical Scientists carry out the interpretation). Taking digital photos at all stages of dissection retains the evidence for the reporting Pathologist. I did this for many years when dissecting samples for my Pathologist; saved drawing diagrams. I guess you'd agree that 80% of all interpretations could be carried out by a Biomedical Scientist (Histotech) once competency is attained and the envelope of responsibility is agreed. It's happened in Cytopathology in the UK! Kemlo Rogerson MSc MIBiol CBiol CSci DMS FIBMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sate Hamza Sent: 18 April 2009 06:37 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Gross Photography As a pathologist, I am a strong proponent of ample gross photography in the cutting room. When I first started in my current place, I thought that not much gross photography was being done. This has increased in recent years in our center. I always encourage our residents to take digital gross photographs. I recently bought an easy to use digital camera and gave it as a gift to our cutting room to encourage more digital photographs. I think that the availability of easy to use digital cameras has made taking pictures much easier. A picture is a great tool for documentation and for communication. No matter how skillful and expressive the gross description is, a picture can make things much easier for sign out. If sections need to be mapped for margins or other considerations, one can take a digital picture, make a quick print out of it and map the sections on it. Such pictures are so helpful, for example, for excisions of flat pigmented lesions from sun-damaged skin. Gross-microscopic correlation can help so much in assessing margin status (this can be so difficult with microscopy alone). It also helps in excisions of vulvar lesions/tumors and in irregularly shaped complex excisions from any site. The digital photos can be taken quickly. They do not need to be textbook quality. The goal usually is to facilitate communication and facilitate the sign-out process. The pictures that our PAs take are placed on a network server in folders that are named with the accession number. The printouts with sections mapped are kept in a binder in the cutting room where a pathologist can find them when the need arises. Sate On Fri, Apr 17, 2009 at 6:30 PM, Joe Nocito wrote: > like Mike, > we only photograph unusual specimens. Seems photographing specimens has > become less and less important. Kind of like autopsies. > -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Sat Apr 18 07:46:18 2009 From: mike <@t> pathview.com (Michael Mihalik) Date: Sat Apr 18 07:46:51 2009 Subject: [Histonet] Gross Photography In-Reply-To: References: <661949901A768E4F9CC16D8AF8F2838C017A3B15@IS-E2K3.grhs.net> <8948BE6AB7D44DB0914DD21F818B10BE@JoePC> <8854ff80904172237t67076079xd8faf2323700ff35@mail.gmail.com> Message-ID: <013f01c9c023$b2afbde0$180f39a0$@com> Just as another endorsement for this practice. Digital images seem so important to us that in our information system, a hyperlink to all images is included in case query. Hence, you can see the image at the same time you're reading all the other details of the case. It's just one more piece of information that helps provide a better diagnosis. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kemlo Sent: Saturday, April 18, 2009 3:01 AM To: 'Sate Hamza'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gross Photography As a Biomedical Scientist I agree with you totally. One of the weaknesses of Biomedical scientists performing the 'grossing' is that the original evidence at dissection is lost to the Pathologist (that is until that Time Biomedical Scientists carry out the interpretation). Taking digital photos at all stages of dissection retains the evidence for the reporting Pathologist. I did this for many years when dissecting samples for my Pathologist; saved drawing diagrams. I guess you'd agree that 80% of all interpretations could be carried out by a Biomedical Scientist (Histotech) once competency is attained and the envelope of responsibility is agreed. It's happened in Cytopathology in the UK! Kemlo Rogerson MSc MIBiol CBiol CSci DMS FIBMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sate Hamza Sent: 18 April 2009 06:37 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Gross Photography As a pathologist, I am a strong proponent of ample gross photography in the cutting room. When I first started in my current place, I thought that not much gross photography was being done. This has increased in recent years in our center. I always encourage our residents to take digital gross photographs. I recently bought an easy to use digital camera and gave it as a gift to our cutting room to encourage more digital photographs. I think that the availability of easy to use digital cameras has made taking pictures much easier. A picture is a great tool for documentation and for communication. No matter how skillful and expressive the gross description is, a picture can make things much easier for sign out. If sections need to be mapped for margins or other considerations, one can take a digital picture, make a quick print out of it and map the sections on it. Such pictures are so helpful, for example, for excisions of flat pigmented lesions from sun-damaged skin. Gross-microscopic correlation can help so much in assessing margin status (this can be so difficult with microscopy alone). It also helps in excisions of vulvar lesions/tumors and in irregularly shaped complex excisions from any site. The digital photos can be taken quickly. They do not need to be textbook quality. The goal usually is to facilitate communication and facilitate the sign-out process. The pictures that our PAs take are placed on a network server in folders that are named with the accession number. The printouts with sections mapped are kept in a binder in the cutting room where a pathologist can find them when the need arises. Sate On Fri, Apr 17, 2009 at 6:30 PM, Joe Nocito wrote: > like Mike, > we only photograph unusual specimens. Seems photographing specimens has > become less and less important. Kind of like autopsies. > -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Sat Apr 18 07:58:35 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Apr 18 07:58:43 2009 Subject: [Histonet] Gross Photography In-Reply-To: <013f01c9c023$b2afbde0$180f39a0$@com> References: <661949901A768E4F9CC16D8AF8F2838C017A3B15@IS-E2K3.grhs.net> <8948BE6AB7D44DB0914DD21F818B10BE@JoePC> <8854ff80904172237t67076079xd8faf2323700ff35@mail.gmail.com> <013f01c9c023$b2afbde0$180f39a0$@com> Message-ID: In government facilities, we are now banned from using flash drives, memory sticks, and other portable devices because some knucklehead at some military installation downloaded a nasty worm that affected many military computers (glad I wasn't that person, probably digging latrines in Iraq or Afghanistan now). This puts us in a tough spot because I was able to shoot pictures in the grossing room. them emailing them to the sign out pathologist. Many times, the path I'm grossing for is out for one reason or another and it helped them see the specimen before I laid blade to specimen. Now, we have to wait for the path to come to the grossing area or put the specimen aside until they can come by. JTT ----- Original Message ----- From: "Michael Mihalik" To: "'kemlo'" ; "'Sate Hamza'" ; Sent: Saturday, April 18, 2009 7:46 AM Subject: RE: [Histonet] Gross Photography > Just as another endorsement for this practice. Digital images seem so > important to us that in our information system, a hyperlink to all images > is > included in case query. Hence, you can see the image at the same time > you're reading all the other details of the case. > > It's just one more piece of information that helps provide a better > diagnosis. > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kemlo > Sent: Saturday, April 18, 2009 3:01 AM > To: 'Sate Hamza'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Gross Photography > > As a Biomedical Scientist I agree with you totally. One of the weaknesses > of > Biomedical scientists performing the 'grossing' is that the original > evidence at dissection is lost to the Pathologist (that is until that Time > Biomedical Scientists carry out the interpretation). Taking digital photos > at all stages of dissection retains the evidence for the reporting > Pathologist. > > I did this for many years when dissecting samples for my Pathologist; > saved > drawing diagrams. I guess you'd agree that 80% of all interpretations > could > be carried out by a Biomedical Scientist (Histotech) once competency is > attained and the envelope of responsibility is agreed. It's happened in > Cytopathology in the UK! > > Kemlo Rogerson MSc MIBiol CBiol CSci DMS FIBMS > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sate Hamza > Sent: 18 April 2009 06:37 > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Gross Photography > > As a pathologist, I am a strong proponent of ample gross photography in > the > cutting room. When I first started in my current place, I thought that not > much gross photography was being done. This has increased in recent years > in > our center. I always encourage our residents to take digital gross > photographs. I recently bought an easy to use digital camera and gave it > as > a gift to our cutting room to encourage more digital photographs. > > I think that the availability of easy to use digital cameras has made > taking > pictures much easier. A picture is a great tool for documentation and for > communication. No matter how skillful and expressive the gross description > is, a picture can make things much easier for sign out. If sections need > to > be mapped for margins or other considerations, one can take a digital > picture, make a quick print out of it and map the sections on it. Such > pictures are so helpful, for example, for excisions of flat pigmented > lesions from sun-damaged skin. Gross-microscopic correlation can help so > much in assessing margin status (this can be so difficult with microscopy > alone). It also helps in excisions of vulvar lesions/tumors and in > irregularly shaped complex excisions from any site. > > The digital photos can be taken quickly. They do not need to be textbook > quality. The goal usually is to facilitate communication and facilitate > the > sign-out process. > > The pictures that our PAs take are placed on a network server in folders > that are named with the accession number. The printouts with sections > mapped > are kept in a binder in the cutting room where a pathologist can find them > when the need arises. > > Sate > > On Fri, Apr 17, 2009 at 6:30 PM, Joe Nocito wrote: > >> like Mike, >> we only photograph unusual specimens. Seems photographing specimens has >> become less and less important. Kind of like autopsies. >> > > > > -- > Sate Hamza, MD, FRCPC > Dermatopathologist > Winnipeg, Canada > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat Apr 18 08:16:31 2009 From: rsrichmond <@t> gmail.com (Robert Richmond) Date: Sat Apr 18 08:16:34 2009 Subject: [Histonet] Re: Gross Photography Message-ID: In my travels as a locum tenens pathologist, I've never seen a pathology service with functioning gross photography, and most of the pathologists I've worked with have been quite hostile to the idea. When a surgeon requests a gross photograph, either an ancient Polaroid camera is hauled out of a forgotten drawer and used to take an out-of-focus print, or else somebody calls Public Relations and half an hour later a dresser-for-successer comes click-click-clicking into the lab in her high heels, goes eeeeyyewww, and takes an out-of-focus photograph using high-end equipment. I've done a fair amount of digital gross photography, using my own camera. Very useful for tumor conferences and the like. Yesterday I shot the photographic protocol for a hospital's tumor board, photographing through the microscope's ocular with my Nikon Coolpix. Bob Richmond Samurai Pathologist Knoxville TN From petepath <@t> yahoo.com Sat Apr 18 08:54:43 2009 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Sat Apr 18 08:54:47 2009 Subject: [Histonet] Gross Photography Message-ID: <655100.51279.qm@web45111.mail.sp1.yahoo.com> I am in full agreement with Dr. Hamza's comments.?I would like to add the impotance of gross photos of any specimens with the potential of medicoleagally?issues. Breast implants, heart valves, any sponges or clamps inadvertently?left inside of anyone?ect. If you smell a lawyer take a picture.. Stephen Peters M.D.?? Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 Phone and fax 201 847 7600 www.pathologyinnovations.com From JWeems <@t> sjha.org Sat Apr 18 09:44:37 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Sat Apr 18 09:43:10 2009 Subject: [Histonet] Gross Photography In-Reply-To: <8948BE6AB7D44DB0914DD21F818B10BE@JoePC> References: <661949901A768E4F9CC16D8AF8F2838C017A3B15@IS-E2K3.grhs.net> <8948BE6AB7D44DB0914DD21F818B10BE@JoePC> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA54781EB@ITSSSXM01V6.one.ads.che.org> We are moving in that direction for grossing - but not to keep everything. We will photograph the specimens so that the pathologist can see exactly what the PA is talking about - and import it into the LIS for viewing. It can be kept if necessary or discarded to save space. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Joy Sent: Friday, April 17, 2009 4:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gross Photography Is there a range of practice among centers as to what specimens are photographed at gross, does anyone photograph pretty much all specimens? Steve Joy, BSc. MLT Research and Development Technologist 5B2.03 Anatomical Pathology University of Alberta Hospital 8440-112 st Edmonton Ab T6G 2B7 Phone: (780) 407-8015 Fax: (780) 407-3009 Email: steven.joy@capitalhealth.ca Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From areed46254 <@t> yahoo.com Sat Apr 18 20:31:19 2009 From: areed46254 <@t> yahoo.com (Amy Reed) Date: Sat Apr 18 20:31:36 2009 Subject: [Histonet] Amy's Calendar Message-ID: Hi I am creating a birthday calendar of all my friends and family. Can you please click on the link below to enter your birthday for me? http://www.birthdayalarm.com/bd2/85011951a953628167b1465146222c89627498d905 Thanks, Amy From Pathrm35 <@t> comcast.net Sun Apr 19 09:20:57 2009 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Sun Apr 19 09:20:59 2009 Subject: [Histonet] HTL (ASCP) seeks employment opportunites In-Reply-To: <594131054.3807551240150752939.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: <24603928.3807971240150857034.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Degreed HTL/QIHC certified tech seeking opportunities in hi volume private labs in the southeastern US. I am a Florida licensed supervisor w/ 20 years experience including dermpath, IHC and lead positions.?Interested in all shifts, IHC,dermpath ?and molecular path opportunities. Please feel free to pass my email along to anyone you know who may be interested. Thanks in advance. No recruiters please! From rjbuesa <@t> yahoo.com Sun Apr 19 09:28:21 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Apr 19 09:28:25 2009 Subject: [Histonet] HTL (ASCP) seeks employment opportunites In-Reply-To: <24603928.3807971240150857034.JavaMail.root@sz0123a.westchester.pa.mail.comcast.net> Message-ID: <445732.61867.qm@web65703.mail.ac4.yahoo.com> In the last issue of Advance for Medical Lab. Professionals there was an advertisement by Quest Diagnostics where they are looking for a supervisor at their Miramar histolab.I think you should contact them ASAP Ren? J. --- On Sun, 4/19/09, Pathrm35@comcast.net wrote: From: Pathrm35@comcast.net Subject: [Histonet] HTL (ASCP) seeks employment opportunites To: histonet@lists.utsouthwestern.edu Date: Sunday, April 19, 2009, 10:20 AM Degreed HTL/QIHC certified tech seeking opportunities in hi volume private labs in the southeastern US. I am a Florida licensed supervisor w/ 20 years experience including dermpath, IHC and lead positions.?Interested in all shifts, IHC,dermpath ?and molecular path opportunities. Please feel free to pass my email along to anyone you know who may be interested. Thanks in advance. No recruiters please! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mscgeary <@t> gmail.com Sun Apr 19 10:25:21 2009 From: mscgeary <@t> gmail.com (Ms C Geary) Date: Sun Apr 19 10:25:28 2009 Subject: [Histonet] training materials/policies/protocols Message-ID: <53ad611e0904190825j2122f36aq1ea92650b10f7278@mail.gmail.com> Dear Rich Not USA based but may provide a useful starting point/resource - training and qualifications set up in the UK by the IBMS. http://www.ibms.org/index.cfm?method=education_and_careers.expert_and_extended_practice&subpage=histological_dissection . Regards Claire Geary Consultant Biomedical Scientist in Cervical Cytology Cytology Dept Addenbrookes Hospital Cambridge Subject: [Histonet] training materials/policies/protocols Hey, Histonet Gurus My pathologist wants me to find any related materials/policies/protocols for training a histo-tech that qualifies through both CLIA/CAP to do grossing. I would greatly appreciate any help I can get as far as either sharing anything or where I can go to find it myself. Thanks Rich Y From putnamj <@t> ggclinic.com Sun Apr 19 11:11:33 2009 From: putnamj <@t> ggclinic.com (Putnam, Jodi) Date: Sun Apr 19 11:11:44 2009 Subject: [Histonet] prostate bx processing time and staining time Message-ID: I was wondering if some of you would share your prostate bx processing times and staining times with me. I am using Richard Allan paraffin type 6. I'm cutting at 4um. Initially the pathologist said that they looked good but a fellow pathologist said that they could be too thick or over stained. I am using the same staining program that I use for all my other derm specimens. Do any of you use a certain program (time) or staining program for prostates? I can adjust the times on the processor to a shorter run or adjust the staining times in H & E but didn't want to just start trouble shooting without some feedback. I am worried that the overnight protocol on the processor might be too much for the bx's. At some labs we used a short run at others we used the over night run but there were other factors like the difference in paraffin etc. Thanks. Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. 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From hodges420 <@t> msn.com Sun Apr 19 20:35:26 2009 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Sun Apr 19 20:35:31 2009 Subject: [Histonet] (no subject) Message-ID: Good evening all, I have a problem and need your input How many blocks and slides should a person cut a day and get gross ready and in the computer or how many blocks and slides should one embed, do specials, immunos and help put gross in the computer both situations in an 8 hour day I have docs that think the PA should have control of gross and ended up with 785 slides cut , have of which a also embedded,specials and immunos. I plan on going to the Lab manger and need some facts Thank you all for your help Tere Hodges _________________________________________________________________ Rediscover Hotmail?: Get e-mail storage that grows with you. http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Storage2_042009 From tifei <@t> foxmail.com Mon Apr 20 01:41:05 2009 From: tifei <@t> foxmail.com (TF) Date: Mon Apr 20 01:41:25 2009 Subject: [Histonet] CNS Fixation References: <4D14F0FC9316DD41972D5F03C070908B017E67FD@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <200904201441004800817@foxmail.com> V2hhdCBhcmUgdGhlIGRpZmZlcmVuY2VzIGJldHdlZW4gYWxjb2hvbGljIGZvcm1hbGluIGFuZCBQ RkEsIE5CRj8NCg0KV2UgaGVyZSBwZXJmdXNlIGJyYWluIHdpdGggNCUgUEZBLCBmb2xsb3dlZCB3 aXRoIDQlIFBGQSBwb3N0LWZpeGF0aW9uIGZvciA2IGhvdXJzLXNldmVyYWwgZGF5cy4NClRoZW4g d2UgY3V0IGJyYWluIGludG8gc2VjdGlvbnMsIG1vdW50IHRvIHNsaWRlcy4NCg0KMjAwOS0wNC0y MCANCg0KDQoNClRGIA0KDQoNCg0Kt6K8/sjLo7ogQnJlZWRlbiwgU2FyYSANCreiy83Ksbzko7og MjAwOS0wNC0xNyAgMjI6NDA6MzcgDQrK1bz+yMujuiBoaXN0b25ldEBsaXN0cy51dHNvdXRod2Vz dGVybi5lZHUgDQqzrcvNo7ogDQrW98zio7ogW0hpc3RvbmV0XSBDTlMgRml4YXRpb24gDQogDQpZ ZXMsIEknbSBiYWNrLiAgSSBuZWVkIHRoZSBleHBlcnRpc2Ugb2YgdGhpcyBncm91cC4NCg0KSSBh bSB0cnlpbmcgdG8gY29udmluY2UgbXkgdGhyZWUgcGF0aG9sb2dpc3RzIHRoYXQgZml4YXRpb24g aW4gYWxjb2hvbGljDQpmb3JtYWxpbiBpcyB0aGUgYmVzdCByb3V0ZSBmb3Igd2hvbGUgYnJhaW4g YW5kIHNwaW5hbCBjb3JkLiAgVGhpcyBoYXMNCmJlZW4gYW4gdXBoaWxsIGJhdHRsZSBhbmQgaW4g b3JkZXIgdG8gcHJldmVudCBzZWN0aW9ucyBmcm9tIHBlZWxpbmcgb2ZmDQpkdXJpbmcgc3RhaW5p bmcsIEkgYW0gc3RpbGwgcHJlLWRyeWluZyBuZWNyb3BzeSBicmFpbiBzbGlkZXMgZm9yIGFuDQpl eHRyYSAyMCBtaW51dGVzIGJlZm9yZSBwdXR0aW5nIG9uIHRoZSBhdXRvLXN0YWluZXIgKGV2ZW4g dGhpcyBkb2VzIG5vdA0KcHJldmVudCBhbGwgaW5zdGFuY2VzKS4gSSBuZWVkIHNvbWUgZXhwZXJ0 IGFtbXVuaXRpb24gZm9yIG15ICJiYXR0bGUiDQpkZXNwaXRlIHRoZSBmYWN0IHRoYXQgdGhlIFNP UCBJJ3ZlIHdyaXR0ZW4gcmVxdWlyZXMgaXQgYW5kIGl0IGlzIHJhcmVseQ0KZm9sbG93ZWQuICBJ ZiBhbGNvaG9saWMgZm9ybWFsaW4gaXMgbm90IGFzIGdvb2QgYXMgc29tZSBvdGhlciBtZXRob2Qs DQpJJ20gb3BlbiB0byBzdWdnZXN0aW9uLiBPZiBjb3Vyc2UsIGNvbnZpbmNpbmcgdGhlbSB0aGF0 IHRoaW5uZXIgc2VjdGlvbnMNCndvdWxkIGFsc28gaGVscCAtIGJ1dCBvbmUgc3RlcCBhdCBhIHRp bWUhICBUaGFuayB5b3UgaW4gYWR2YW5jZSENCg0KU2FsbHkgQnJlZWRlbiwgSFQoQVNDUCkNCk5N IERlcHQuIG9mIEFncmljdWx0dXJlDQpWZXRlcmluYXJ5IERpYWdub3N0aWMgU2VydmljZXMNClBP IEJveCA0NzAwDQpBbGJ1cXVlcnF1ZSwgTk0gIDg3MTA2DQo1MDUtODQxLTI1NzYNCg0KX19fX19f X19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX18NCkhpc3RvbmV0IG1haWxp bmcgbGlzdA0KSGlzdG9uZXRAbGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1DQpodHRwOi8vbGlzdHMu dXRzb3V0aHdlc3Rlcm4uZWR1L21haWxtYW4vbGlzdGluZm8vaGlzdG9uZXQNCg== From tifei <@t> foxmail.com Mon Apr 20 01:42:46 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Mon Apr 20 01:43:12 2009 Subject: =?utf-8?B?UmU6IFJlOiBbSGlzdG9uZXRdIENyeW9zdGF0IHNlY3Rpb25zIG1vdW50aW5nIHdpdGggMC4xTSBQQiBzb2x1dGlvbiBvbnRvIHNsaWRlcw==?= References: , <200904032342579533934@foxmail.com>, <908441.53282.qm@web1107.biz.mail.sk1.yahoo.com> Message-ID: <200904201442413184650@foxmail.com> How about to use a drop of water? Sometimes the section is a bit big, and it is hard to mount the section very flat on slides without a bubble... 2009-04-20 TF ???? Paula Pierce ????? 2009-04-03 23:59:43 ???? tifei ??? ??? Re: [Histonet] Cryostat sections mounting with 0.1M PB solution onto slides Hi TF, you should not place the drop of buffer on the slide under the section. The section will wash off. You will get buffer crystals under the section during drying and although they will dissolve out later, they will cause the section to not stick to the slide where they are. Place the section directly on a warm (room temperature) slide and the section will adhere. Paula From: TF To: Histonet Sent: Friday, April 3, 2009 10:43:03 AM Subject: [Histonet] Cryostat sections mounting with 0.1M PB solution onto slides After we cut the brain sections on a cryostat (OCT embedded), we brush a drop of 0.1M PB on to the slide before mounting the sections. Do anyone know the side effect of this - for example, the sections will peel off during immunostaining? Another question is about the dry time after mounting and before staining. I know some people asked similar questions before, but they are using fresh tissue for frozen sections. We here perfuse the animal, post-fix the tissue and sink it in sucrose before making cryostat sections. So we may dry the sections in air up to weeks. I would like to ask about the proper heating time (with a heat plate) before dry the sections up in a fumehood. Should we heat the sections at 60 degree for 10 min or 2 hours shortly after mounting? Thanks very much. 2009-04-03 TF _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon Apr 20 08:06:44 2009 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Apr 20 08:06:48 2009 Subject: [Histonet] Re: Gross Photography In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D83153@EMAIL.archildrens.org> We are fortunate to have a wonderful gross photography set up. Our residents take many pictures. The photos are downloaded and kept in our LIS system. One nice thing for taking photos is for the mapping of bone tumors. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Saturday, April 18, 2009 8:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Gross Photography In my travels as a locum tenens pathologist, I've never seen a pathology service with functioning gross photography, and most of the pathologists I've worked with have been quite hostile to the idea. When a surgeon requests a gross photograph, either an ancient Polaroid camera is hauled out of a forgotten drawer and used to take an out-of-focus print, or else somebody calls Public Relations and half an hour later a dresser-for-successer comes click-click-clicking into the lab in her high heels, goes eeeeyyewww, and takes an out-of-focus photograph using high-end equipment. I've done a fair amount of digital gross photography, using my own camera. Very useful for tumor conferences and the like. Yesterday I shot the photographic protocol for a hospital's tumor board, photographing through the microscope's ocular with my Nikon Coolpix. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Imcgonnell <@t> RVC.AC.UK Mon Apr 20 08:38:19 2009 From: Imcgonnell <@t> RVC.AC.UK (McGonnell, Imelda Mary) Date: Mon Apr 20 08:38:26 2009 Subject: [Histonet] suppliers of chlorantine fast red 5B Message-ID: <193A454735ABFE438D08FA139B54B005058AA258@cmw2kex01.rvc.ac.uk> I am working on developing bone in the chick embryo and have a dwindling stock of chlorantine fast red 5B solution, which is a good marker of developing bone in histological sections. Does anyone know of a current supplier of this chemical, I am finding it very hard to find one. Many thanks Imelda Dr Imelda McGonnell Lecturer Dept. Veterinary Basic Sciences Royal Veterinary College Royal College St London NW1 0TU Phone : Extn. 5453 Direct dial 020 7468 1223 From bhewlett <@t> cogeco.ca Mon Apr 20 09:09:33 2009 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon Apr 20 09:09:34 2009 Subject: [Histonet] suppliers of chlorantine fast red 5B References: <193A454735ABFE438D08FA139B54B005058AA258@cmw2kex01.rvc.ac.uk> Message-ID: Imelda, Chlorantine fast red 5B, AKA direct red 81 and Sirius red 4B, CI 28160 is listed by VWR International UK(tel 800-932-5000). Bryan ----- Original Message ----- From: "McGonnell, Imelda Mary" To: Sent: Monday, April 20, 2009 9:38 AM Subject: [Histonet] suppliers of chlorantine fast red 5B I am working on developing bone in the chick embryo and have a dwindling stock of chlorantine fast red 5B solution, which is a good marker of developing bone in histological sections. Does anyone know of a current supplier of this chemical, I am finding it very hard to find one. Many thanks Imelda Dr Imelda McGonnell Lecturer Dept. Veterinary Basic Sciences Royal Veterinary College Royal College St London NW1 0TU Phone : Extn. 5453 Direct dial 020 7468 1223 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Apr 20 09:18:53 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 20 09:18:57 2009 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: <310437.97796.qm@web65703.mail.ac4.yahoo.com> Mary: The averages are 25 blocks/hour sectioning and 60 blocks/hour embedding. Under separate cover I am sending productivity values for other tasks. Another thing is what you are expecting of the multi-tasking, take-care-of-everything person you are describing. That is a special situation and the standards probably will be different to those I will send you. Ren? J. --- On Sun, 4/19/09, MARY T HODGES wrote: From: MARY T HODGES Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Date: Sunday, April 19, 2009, 9:35 PM Good evening all, I have a problem and need your input How many blocks and slides should a person cut a day and get gross ready and in the computer or how many blocks and slides should one embed, do specials, immunos and help put gross in the computer both situations in an 8 hour day I have docs that think the PA should have control of gross and ended up with 785 slides cut , have of which a also embedded,specials and immunos. I plan on going to the Lab manger and need some facts Thank you all for your help Tere Hodges _________________________________________________________________ Rediscover Hotmail?: Get e-mail storage that grows with you. http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Storage2_042009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robert_schoonhoven <@t> yahoo.com Mon Apr 20 11:11:48 2009 From: robert_schoonhoven <@t> yahoo.com (Robert Schoonhoven) Date: Mon Apr 20 11:11:51 2009 Subject: [Histonet] brdu on ffpe rat fixed for up to 2 years in NBF In-Reply-To: References: Message-ID: <504850.49621.qm@web31108.mail.mud.yahoo.com> Patsy, If the animal was treated with BrdU initially, then yes I have. Unfortunately All of my papers and reference materials are boxed up at the moment (preparing to move) but I have done this procedure so many times that I can pretty much reconstruct it from memory. 1) Hydrate slides to ddH2O as per SOP. 2) Hydrolyze with 4N HCl at 37oC for 20 minutes. 3) Rinse with ddH2O for 1 minute at RT. 4) Transfer slides to a staining dish filled with ddH2O (kept at 37oC) for 5 minutes. 5) Incubate in pepsin solution (Dako) for 15 minutes at 37oC. All of the remaining steps are at room temp. 6) Rinse 2X with ddH2O, 1 minute each rinse. 7) Rinse 2X with PBSt, 3 minutes each rinse 8) Peroxidase Blocking Reagent (Dako), 5 minute incubation. 9) Repeat step #7. 10) Primary antibody (anti-BrdU), incubate 10 minutes at a 1:200 dilution. [5 ?L antibody to 995 ?L 1% BSA in PBS] 11) Repeat step #7. 12) Polymer labeled secondary antibody (Dako Envision HRP), incubate for 10 minutes. 13) Repeat step #7. 14) Incubate in working Dako DAB solution for 8 minutes (1 drop DAB Chromogen per 1 ml substrate buffer). 15) Rinse well with ddH2O. 16) DAB enhancer solution (2.5% CoCl2 , 2.0% NiSO4(NH4)2SO4 in ddH2O) for 8 minutes 17) Repeat step #15. 18) Richard Allan Hematoxylin 1 for 25 seconds, rinse with tap water until there is no blue color in the water. 19) Let sit in tap water for 5 minutes. 20) dehydrate and coverslip in accordance with SOP?s. Best of luck, Bob Schoonhoven ________________________________ From: Patsy Ruegg To: histonet@lists.utsouthwestern.edu Sent: Friday, April 17, 2009 1:26:36 PM Subject: [Histonet] brdu on ffpe rat fixed for up to 2 years in NBF Anybody tried doing IHC for brdu on ffpe rat intestine and skin fixed for up to 2 years in NBF? Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Mon Apr 20 11:17:48 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Mon Apr 20 11:17:58 2009 Subject: [Histonet] protease XIV Message-ID: I'm trying to find a source for protease XIV other than Sigma. Any suggestions? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From cforster <@t> umn.edu Mon Apr 20 11:32:32 2009 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Apr 20 11:32:35 2009 Subject: [Histonet] CNS Fixation In-Reply-To: <417666.16473.qm@web63704.mail.re1.yahoo.com> References: <4D14F0FC9316DD41972D5F03C070908B017E67FD@nmdamailsvr.nmda.ad.nmsu.edu> <5D64396A0D4A5346BEBC759022AAEAA547810C@ITSSSXM01V6.one.ads.che.org> <417666.16473.qm@web63704.mail.re1.yahoo.com> Message-ID: <49ECA3A0.4010607@umn.edu> Animal brain is the same. IF you aior dry overnight and then into the oven before starting your stains you will not lose sections. Colleen Forster U of MN Barbara Albert wrote: > We've found this also. Even 30-60 minutes air drying helps alot with human brain. We use regular slides and NBF. > > Barbara Albert > UCSF Medical Center > San Francisco > > > > > ________________________________ > From: "Weems, Joyce" > To: "Breeden, Sara" ; histonet@lists.utsouthwestern.edu > Sent: Friday, April 17, 2009 7:49:49 AM > Subject: RE: [Histonet] CNS Fixation > > I have found that brain sections that are air-dried overnight - then > dried in the oven rarely ever wash off. We fix in 10% NBF. This is human > - not sure if animal would be different. > > Best, j > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, > Sara > Sent: Friday, April 17, 2009 10:37 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CNS Fixation > > Yes, I'm back. I need the expertise of this group. > > > > I am trying to convince my three pathologists that fixation in alcoholic > formalin is the best route for whole brain and spinal cord. This has > been an uphill battle and in order to prevent sections from peeling off > during staining, I am still pre-drying necropsy brain slides for an > extra 20 minutes before putting on the auto-stainer (even this does not > prevent all instances). I need some expert ammunition for my "battle" > despite the fact that the SOP I've written requires it and it is rarely > followed. If alcoholic formalin is not as good as some other method, > I'm open to suggestion. Of course, convincing them that thinner sections > would also help - but one step at a time! Thank you in advance! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From mitchellja <@t> email.chop.edu Mon Apr 20 11:48:06 2009 From: mitchellja <@t> email.chop.edu (Janice Mitchell) Date: Mon Apr 20 11:48:45 2009 Subject: [Histonet] Good Afternoon, Anyone having trouble with fiber contamination from disposable cytofunnels filter? Sometimes it is worse than others and it is interferring with our silver stains to the point where they are unreadable. We used to buy the individually wrapped funnels but, recently we have been using the bulk supply. We think this could be the problem but we are not sure. Any suggestions? Thanks, Janice Message-ID: Good Afternoon, Anyone having trouble with fiber contamination from disposable cytofunnels filter? Sometimes it is worse than others and it is interferring with our silver stains to the point where they are unreadable. We used to buy the individually wrapped funnels but, recently we have been using the bulk supply. We think this could be the problem but we are not sure. Any suggestions? Thanks, Janice From katherine-walters <@t> uiowa.edu Mon Apr 20 13:18:44 2009 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Mon Apr 20 13:18:59 2009 Subject: [Histonet] BRDU, tetracycline and calcein IV in bone Message-ID: This may be a question for Gayle, or someone else who specializes in bone. I will be doing a project involving the facial bones of 8 week-old rabbits. We want to look at regeneration of bone in this area. I have some experience with BRDU in mouse tissues. My questions are: 1.) Will decalcification affect the BRDU signal? If not, what is the best method for decalcification? Approximately how long? 2.) Do I need to use a ground section for tetracycline and calcein IV or can I decalcify and paraffin embed? 3.) What is the best fixation procedure? 4.) Anything I need to know, but am too naive to ask? Thanks for any information you can share! Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 katherine-walters@uiowa.edu www.uiowa.edu/~cemrf Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. From sjchtascp <@t> yahoo.com Mon Apr 20 14:28:08 2009 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Mon Apr 20 14:28:11 2009 Subject: [Histonet] Looking for Pool Work Message-ID: <285680.40896.qm@web38207.mail.mud.yahoo.com> I live in south central WI just south of Madison, WI and just north of Rockford, Ill.? I'm looking for pool or as needed work as an HT or Mohs Tech.? I will consider all work within a 100 mile radius.? Short-term outside of this area.? I've done clinical and research Histology and?am experienced in Mohs cryosectioning. ? Respectfully, ? Steven Coakley 608-879-9556 From kerry.l.crabb <@t> gsk.com Mon Apr 20 17:31:51 2009 From: kerry.l.crabb <@t> gsk.com (kerry.l.crabb@gsk.com) Date: Mon Apr 20 17:32:07 2009 Subject: [Histonet] Mold for OCT samples Message-ID: I've had a request for a mold that is large enough to freeze a cross section of human jejunum in OCT. Suggestions would be appreciated. Kerry From immrstambo <@t> hotmail.com Mon Apr 20 18:03:23 2009 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Mon Apr 20 18:03:27 2009 Subject: [Histonet] Mold for OCT samples In-Reply-To: References: Message-ID: HAPPY LAB WEEK EVERYONE! HOPE YOU ALL GET TREATED WELL!!! Christine Tambasco, HT (ASCP) ALbany Medical Center NY > To: histonet@lists.utsouthwestern.edu > From: kerry.l.crabb@gsk.com > Date: Mon, 20 Apr 2009 18:31:51 -0400 > Subject: [Histonet] Mold for OCT samples > > I've had a request for a mold that is large enough to freeze a cross > section of human jejunum in OCT. Suggestions would be appreciated. > Kerry > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows Live?: Keep your life in sync. http://windowslive.com/explore?ocid=TXT_TAGLM_WL_allup_1a_explore_042009 From vapatpxs <@t> yahoo.com Mon Apr 20 18:07:37 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Mon Apr 20 18:07:43 2009 Subject: [Histonet] OptiQuip Model 1200 Message-ID: <801623.76495.qm@web46101.mail.sp1.yahoo.com> Hello Everyone, In my continuing quest to find out how to finish putting the bulb assembly back together on my fluorescence microscope I've found out some additional information. The power supply and bulb assembly were made by a company called "OptiQuip". I have called the company, which still exists, but have yet to receive a return phone call. Anyway, if you have such a beasty and have put the bulb assembly back together, it holds either a xenon or mercury bulb, please let me know. Even OptiQuip's owner's manual does not have a drawing or explain it clearly enough for me to understand. Once more with feeling, Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 From thisisann <@t> aol.com Mon Apr 20 21:17:29 2009 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Mon Apr 20 21:17:56 2009 Subject: [Histonet] Peloris Tissue Processor Message-ID: <8CB903E74C96EB0-1248-D8A@webmail-dd16.sysops.aol.com> Does anyone use recycled and/or fresh Formula 83 as a clearant on their Peloris Tissue Processor?? If so, can you tell me what final threshold percentage you use. Thanks, Ann From petepath <@t> yahoo.com Mon Apr 20 21:59:02 2009 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Mon Apr 20 21:59:06 2009 Subject: [Histonet] Mold for OCT samples Message-ID: <345976.62040.qm@web45103.mail.sp1.yahoo.com> Hi Kerry, I have well bars with wells of? 30 x 50 mm 30 x 30 mm which will should handle your task. I can also custom make larger ones if necessary if you an wait a few months. I offer all colleagues to first demo the apparatus with?no obligation. If you are not familiar with my sytem visit: http://www.pathologyinnovations.com/index.html to learn about the Precision Cryoembedding System. ? Stephen Peters M.D.? 201 847 0052 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 Phone and fax 201 847 7600 www.pathologyinnovations.com From ooi.ting.huay <@t> nhc.com.sg Tue Apr 21 00:33:18 2009 From: ooi.ting.huay <@t> nhc.com.sg (ooi.ting.huay@nhc.com.sg) Date: Tue Apr 21 00:33:52 2009 Subject: [Histonet] Cloudy MMA embedded block Message-ID: Hi, I am trying very hard to get a clear MMA embedded block. I am appreciate if there is any advice on doing it. I am using 100% MMA (liquid) and 0.5% Perkadox 16 (powder) to do the embedding. I have tried to embed the sample in different conditions. However, it seems to be extremely hard to get a clear embedded block. Below are the methods that I have tried. 1. Before embedding, bring the MMA and perkadox 16 to room temperature. After a few mix it by invertion, I aliquot the solution to the glass vials that contain samples or simply glass vials that without sample. I put the glass vials (both with and without sample) in 4 degree, room temperature and even 37degree oven. 2. Mix the MMA and perkadox when it is cold, invert it a few times. Aliquot it to glass vials that contain sample or without sample. Put in 4degree, room temperature and 37degree oven. I have also tried to speed up the polymerization process by using vacumm for a few samples... Throughout all the sample that I have, I only manage to get a clear empty glass vial which I put in the 4degree for one month and a clear embedded sample which I put in 4 degree overnight and bring it out to room temperature. The rest of the glass vials (with or without sample) are cloudy. I have repeated the methods that I used to get a clear embedded block, I did not get any clear block (for both with and without sample). I am glad if there is any expert advice or guidance. We need a clear embedded sample. Thank you very much! Regards, Ooi _________________________________________________ Confidential information. Unauthorized use or disclosure prohibited. Refer http://www.singhealth.com.sg/ContactUs/#Disclaimer From yvan_lindekens <@t> yahoo.com Tue Apr 21 05:14:12 2009 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Tue Apr 21 05:14:18 2009 Subject: [Histonet] Affordable microtome knife reconditioning in the EC? Message-ID: <617698.83386.qm@web110212.mail.gq1.yahoo.com> Hi all, I have some large (25 - 30cm) type B, C and D, both steel and tungsten carbide, microtome knives that need sharpening and/or reconditioning. Does anyone knows a company in Europe (preferably within the EC) that does that kind of work at an affordable price? By the way: what is a *normal* price for those things? Impossible to answer question, I suppose... Thanks in advance! Yvan. From yvan_lindekens <@t> yahoo.com Tue Apr 21 05:28:46 2009 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Tue Apr 21 05:28:50 2009 Subject: [Histonet] Replacement glass plates for Reichert Microtome Knife Sharpener 903? Message-ID: <902069.70864.qm@web110214.mail.gq1.yahoo.com> Hi all, It struck me that all those microtome knife sharpeners (AO 935, Reichert 903, AO 903...) all look very much the same. Are the glass plates interchangeable? Would the glass plates for the current Leica sharpener SP9000 (part.no. 14041819698, dimensions 292mm x 127mm x 6 mm) fit the Reichert Microtome Knife Sharpener 903? Thanks in advance for your answers! Yvan. From ratliffjack <@t> hotmail.com Tue Apr 21 07:48:48 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Apr 21 07:48:57 2009 Subject: [Histonet] Affordable microtome knife reconditioning in the EC? In-Reply-To: <617698.83386.qm@web110212.mail.gq1.yahoo.com> References: <617698.83386.qm@web110212.mail.gq1.yahoo.com> Message-ID: I don't know about Europe, but here in the US there are a few vendors that provide these services (Dorn and Hart Microedge, Sturkey, and Delaware Diamond Knives). I am not sure of the pricing for all three of these vendors, but for the past 11 years I have sent my D-profile rotary wedge and sledge/polycut tungsten-carbide knives for re- sharpening services to Dorn and Hart Microedge. They are located near Chicago, Illinois and their prices are very reasonable. Maybe you can google all three vendors for information if you get in a bind, but I am pretty sure they provide services outside the US. Jack On Apr 21, 2009, at 5:14 AM, yvan lindekens wrote: > > Hi all, > > I have some large (25 - 30cm) type B, C and D, both steel and > tungsten carbide, microtome knives that need sharpening and/or > reconditioning. > > Does anyone knows a company in Europe (preferably within the EC) > that does that kind of work at an affordable price? > > By the way: what is a *normal* price for those things? Impossible to > answer question, I suppose... > > Thanks in advance! > > Yvan. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Samuel_Perry <@t> DFCI.HARVARD.EDU Tue Apr 21 08:13:02 2009 From: Samuel_Perry <@t> DFCI.HARVARD.EDU (Perry, Samuel) Date: Tue Apr 21 08:13:07 2009 Subject: [Histonet] melanoma markers on mouse Message-ID: Hi All, I am trying to perform IHC for melanoma markers on mouse tissue. In particular, does anyone have hands-on experience with antibodies against Pmel17/Silv/gp100 (recognized by HMB-45 antibody in humans), Melan-A/MART-1, or MITF on mouse? Thanks Sam Perry Research Technician DFCI, Boston MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From ratliffjack <@t> hotmail.com Tue Apr 21 08:24:29 2009 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Tue Apr 21 08:24:39 2009 Subject: [Histonet] Cloudy MMA embedded block In-Reply-To: References: Message-ID: Ooi, Somehow you are getting moisture/water mixed in with the MMA solution(s). I only remember visiting the IMCB facility, so if this is not your facility then I am not sure of your laboratory environment (air temperature/humidity). The best thing I can tell you at this point is to try and find ways to reduce the chance for moisture accumulation. I am pretty sure that at a minimum you are storing your catalyst and monomer (MMA) at 4C and your softner (DBP) at room temp. Maybe you should take extra steps to ensure your chemicals are at room temp before any use. Lastly, your polymerization is taking too long for your small 20mL vials. My specimens of similar size require only 3-5 days to completely polymerize. Now, I dont remember your concentration of catalyst, but if it is around 2.5g/1L embedding solution, try skipping the 4C step and use room temp (<23C) until the specimens are polymerized. Then finish them off in the oven when they are tacky or mostly hard on top. The 4C step is mostly to slow the polymerization for larger specimens requiring a larger volume of solution and/or when the lab temp cannot be controlled routinely at or below 23C. The water bath then also helps to regulate the temperature of the polymerization in the case where the lab environment is too cool (<20C) and retarding the rate of polymerzation. The main thing to keep in mind is that the lab environment pretty much controls the process and is probably the main variable that will contrast labs that perform the exact same method. Jack On Apr 21, 2009, at 12:33 AM, ooi.ting.huay@nhc.com.sg wrote: > Hi, > > I am trying very hard to get a clear MMA embedded block. I am > appreciate > if there is any advice on doing it. I am using 100% MMA (liquid) and > 0.5% > Perkadox 16 (powder) to do the embedding. I have tried to embed the > sample > in different conditions. However, it seems to be extremely hard to > get a > clear embedded block. > > Below are the methods that I have tried. > > 1. Before embedding, bring the MMA and perkadox 16 to room > temperature. > After a few mix it by invertion, I aliquot the solution to the glass > vials > that contain samples or simply glass vials that without sample. I > put the > glass vials (both with and without sample) in 4 degree, room > temperature > and even 37degree oven. > > 2. Mix the MMA and perkadox when it is cold, invert it a few times. > Aliquot it to glass vials that contain sample or without sample. Put > in > 4degree, room temperature and 37degree oven. > > I have also tried to speed up the polymerization process by using > vacumm > for a few samples... > > Throughout all the sample that I have, I only manage to get a clear > empty > glass vial which I put in the 4degree for one month and a clear > embedded > sample which I put in 4 degree overnight and bring it out to room > temperature. The rest of the glass vials (with or without sample) are > cloudy. > > I have repeated the methods that I used to get a clear embedded > block, I > did not get any clear block (for both with and without sample). > > I am glad if there is any expert advice or guidance. We need a clear > embedded sample. Thank you very much! > > > > Regards, > Ooi > _________________________________________________ > Confidential information. Unauthorized use or disclosure prohibited. > Refer http://www.singhealth.com.sg/ContactUs/#Disclaimer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mucram11 <@t> comcast.net Tue Apr 21 08:45:05 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Tue Apr 21 08:45:09 2009 Subject: [Histonet] Cloudy MMA embedded block In-Reply-To: Message-ID: <698272155.25811240321505599.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Hi, We don't store our MMA or DBP in the refirgerator as the movement between 4C and room temperature can cause condensation in the bottles.? This just adds water to the material and as it is cooled and re-warmed you are adding more each time and in both directions.?? When it comes to room temperature and then is replaced in the refrigerator it again will develop condensation.? Over time the MMA will have enough water in it to become discolored.? I am surprised you are not seeing the discoloration in the MMA liquid as it poured as a warning not to use it.? We have fair control of temperature in the laboratory and are generally between 68F to 75F with the average as around 70F.? We have never had a problem with the MMA polymerizing or developing a water content.? It is stored in a dark cabinet that is in an area I would say is a little cooler than the room.? We generally don't polymerize in the cold at 4C however, you timing seems long for the process.? It is best not to disturb the vials too much once you are in the embedding phase.? Movement or inversion of the vials can cause a problem as the MMA will polymerize from the bottom up and remixing it can slow the process.? Also make sure you tops are tight at this step. Pam Marcum UPENN Vet Sch New Botlon Center ----- Original Message ----- From: "Jack Ratliff" To: "Ooi Ting Huay" , " " Sent: Tuesday, April 21, 2009 9:24:29 AM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] Cloudy MMA embedded block Ooi, Somehow you are getting moisture/water mixed in with the MMA ? solution(s). I only remember visiting the IMCB facility, so if this is ? not your facility then I am not sure of your laboratory environment ? (air temperature/humidity). The best thing I can tell you at this ? point is to try and find ways to reduce the chance for moisture ? accumulation. I am pretty sure that at a minimum you are storing your catalyst and ? monomer (MMA) at 4C and your softner (DBP) at room temp. Maybe you ? should take extra steps to ensure your chemicals are at room temp ? before any use. Lastly, your polymerization is taking too long for ? your small 20mL vials. My specimens of similar size require only 3-5 ? days to completely polymerize. Now, I dont remember your concentration ? of catalyst, but if it is around 2.5g/1L embedding solution, try ? skipping the 4C step and use room temp (<23C) until the specimens are ? polymerized. Then finish them off in the oven when they are tacky or ? mostly hard on top. The 4C step is mostly to slow the polymerization ? for larger specimens requiring a larger volume of solution and/or when ? the lab temp cannot be controlled routinely at or below 23C. The water ? bath then also helps to regulate the temperature of the polymerization ? in the case where the lab environment is too cool (<20C) and retarding ? the rate of polymerzation. The main thing to keep in mind is that the lab environment pretty much ? controls the process and is probably the main variable that will ? contrast labs that perform the exact same method. Jack On Apr 21, 2009, at 12:33 AM, ooi.ting.huay@nhc.com.sg wrote: > Hi, > > I am trying very hard to get a clear MMA embedded block. I am ? > appreciate > if there is any advice on doing it. I am using 100% MMA (liquid) and ? > 0.5% > Perkadox 16 (powder) to do the embedding. I have tried to embed the ? > sample > in different conditions. However, it seems to be extremely hard to ? > get a > clear embedded block. > > Below are the methods that I have tried. > > 1. Before embedding, bring the MMA and perkadox 16 to room ? > temperature. > After a few mix it by invertion, I aliquot the solution to the glass ? > vials > that contain samples or simply glass vials that without sample. I ? > put the > glass vials (both with and without sample) in 4 degree, room ? > temperature > and even 37degree oven. > > 2. Mix the MMA and perkadox when it is cold, invert it a few times. > Aliquot it to glass vials that contain sample or without sample. Put ? > in > 4degree, room temperature and 37degree oven. > > I have also tried to speed up the polymerization process by using ? > vacumm > for a few samples... > > Throughout all the sample that I have, I only manage to get a clear ? > empty > glass vial which I put in the 4degree for one month and a clear ? > embedded > sample which I put in 4 degree overnight and bring it out to room > temperature. The rest of the glass vials (with or without sample) are > cloudy. > > I have repeated the methods that I used to get a clear embedded ? > block, I > did not get any clear block (for both with and without sample). > > I am glad if there is any expert advice or guidance. We need a clear > embedded sample. Thank you very much! > > > > Regards, > Ooi > _________________________________________________ > Confidential information. Unauthorized use or disclosure prohibited. > Refer http://www.singhealth.com.sg/ContactUs/#Disclaimer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From putnamj <@t> ggclinic.com Tue Apr 21 09:34:55 2009 From: putnamj <@t> ggclinic.com (Putnam, Jodi) Date: Tue Apr 21 09:35:02 2009 Subject: [Histonet] rulers Message-ID: Thanks to everyone that has given me feedback on the prostate situation. I am testing a shorter run today and will hope that solves the issue. I was wondering if anyone (vendors included) knew of anywhere I could get some of the cheap plastic rulers (the kind they give away usually) that are flexible. I use them to measure my skin lesions and I had a few that seem to have walked away. I am using my scalpel handle currently to measure (in cm) but would really love some of the plastic ones. Thanks and have a great day. Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From suhyoung.jeong <@t> gmail.com Tue Apr 21 09:51:35 2009 From: suhyoung.jeong <@t> gmail.com (Suhyoung Jeong) Date: Tue Apr 21 09:51:38 2009 Subject: [Histonet] Vectashield mounting media problem? Message-ID: <450012a20904210751ja20d62ai897ade0df3e1d9e7@mail.gmail.com> Hello everyone, Our lab has been using 'Vectashield mounting media w/ DAPI' for many years and had no problem of fading. Recently, two bottles with different lot numbers in a row gone bad in around four months (the signal disappears in front of your eyes, tested on a couple of different microscopes). 1) Has anyone experienced same problem? Vector lab only says that this is a very stable product and should not be happening when stored at 4C. 2) Can anyone recommend a good aqueous mounting media with DAPI for immunofluorescence? Thank you in advance, Regards, Suh From Montina.VanMeter <@t> pbrc.edu Tue Apr 21 09:55:25 2009 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Tue Apr 21 09:56:19 2009 Subject: [Histonet] Vectashield mounting media problem? In-Reply-To: <450012a20904210751ja20d62ai897ade0df3e1d9e7@mail.gmail.com> References: <450012a20904210751ja20d62ai897ade0df3e1d9e7@mail.gmail.com> Message-ID: <4FE7FB862E90E448AE32388E759220E5012CD6E8@pbrcas31.pbrc.edu> ProLong Gold w/ DAPI (Invitrogen/Molecular Probes) This is a permanent mounting media - works great! Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suhyoung Jeong Sent: Tuesday, April 21, 2009 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Vectashield mounting media problem? Hello everyone, Our lab has been using 'Vectashield mounting media w/ DAPI' for many years and had no problem of fading. Recently, two bottles with different lot numbers in a row gone bad in around four months (the signal disappears in front of your eyes, tested on a couple of different microscopes). 1) Has anyone experienced same problem? Vector lab only says that this is a very stable product and should not be happening when stored at 4C. 2) Can anyone recommend a good aqueous mounting media with DAPI for immunofluorescence? Thank you in advance, Regards, Suh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Tue Apr 21 10:43:59 2009 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Tue Apr 21 10:44:04 2009 Subject: [Histonet] Vectashield mounting media problem? In-Reply-To: <450012a20904210751ja20d62ai897ade0df3e1d9e7@mail.gmail.com> References: <450012a20904210751ja20d62ai897ade0df3e1d9e7@mail.gmail.com> Message-ID: <7ED48E50DA675B1CC66DFF39@bchwxp2702.ad.med.buffalo.edu> We had the EXACT same problem about a year ago! We don't believe we had storage issues, either. We've been using SlowFade Gold (Molecular Probes, Invitrogen) for about a year now and it's been a great aqueous mounting medium. We add our own DAPI (2ul of a 1mg/ml DAPI solution per 500ul SlowFade) and it works fine. Merced --On Tuesday, April 21, 2009 10:51 AM -0400 Suhyoung Jeong wrote: > Hello everyone, > > Our lab has been using 'Vectashield mounting media w/ DAPI' for many years > and had no problem of fading. Recently, two bottles with different lot > numbers in a row gone bad in around four months (the signal disappears in > front of your eyes, tested on a couple of different microscopes). > > 1) Has anyone experienced same problem? Vector lab only says that this is > a very stable product and should not be happening when stored at 4C. > > 2) Can anyone recommend a good aqueous mounting media with DAPI for > immunofluorescence? > > Thank you in advance, > > Regards, Suh > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. From Shirley.Chu <@t> moldev.com Tue Apr 21 10:46:29 2009 From: Shirley.Chu <@t> moldev.com (Chu, Shirley) Date: Tue Apr 21 11:03:20 2009 Subject: [Histonet] Reminder - California Society for Histotechnology Annual Symposium Message-ID: Just a reminder, registration deadline for the upcoming California Society for Histotechnology Annual Symposium/Convention is May 1st. This is a 3.5 day education forum on May 14-17 and will be held at the Westin San Francisco Airport. For further information, please see our website at: www.californiahistology.org or contact: Shirley Chu at 408-747-3765 or by email at: shirley.chu@moldev.com. Cut-off date for hotel registration is April 22nd (online hotel registration: http://www.starwoodmeeting.com/StarGroupsWeb/res?id=0806209635&key=F3921 ) Shirley Chu Application Scientist, Arcturus LCM Products Molecular Devices (now a part of MDS Analytical Technologies) 408-747-3765 | www.moleculardevices.com This e-mail and any files transmitted with it may contain privileged and/or confidential information and may be read or used only by the intended recipient. If you are not the intended recipient of the e-mail or any of its attachments, please be advised that you have received this e-mail in error and any use, dissemination, distribution, forwarding, printing or copying of this e-mail or any attached files is strictly prohibited. If you have received this e-mail in error, please immediately purge it and all attachments and notify the sender by reply e-mail or contact the sender at the number listed. From jcline <@t> wchsys.org Tue Apr 21 11:32:37 2009 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue Apr 21 11:32:44 2009 Subject: [Histonet] Peloris Tissue Processor In-Reply-To: <8CB903E74C96EB0-1248-D8A@webmail-dd16.sysops.aol.com> Message-ID: <4B4B66F6AFEB4E518679996481312ED8@wchsys.org> You cannot use recycled Formula 83 in the Peloris. The threshold is 88%. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com Sent: Monday, April 20, 2009 10:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peloris Tissue Processor Does anyone use recycled and/or fresh Formula 83 as a clearant on their Peloris Tissue Processor?? If so, can you tell me what final threshold percentage you use. Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From kelly_colpitts <@t> hotmail.com Tue Apr 21 11:43:59 2009 From: kelly_colpitts <@t> hotmail.com (Kelly Colpitts) Date: Tue Apr 21 11:44:03 2009 Subject: [Histonet] Reusing specimen containers Message-ID: Hi Histoland! I'm just wondering what folks out there are doing about specimen containers. Is anyone cleaning them out and reusing them? Is there any CAP or Joint Commission regulations that say that all specimen containers can only be used once or can you reuse them as long as they have been thoroughly cleaned? Thanks for you all your input, Kelly _________________________________________________________________ Windows Live? SkyDrive?: Get 25 GB of free online storage. http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_skydrive_042009 From Jim.Chalmers <@t> leica-microsystems.com Tue Apr 21 11:48:45 2009 From: Jim.Chalmers <@t> leica-microsystems.com (Jim.Chalmers@leica-microsystems.com) Date: Tue Apr 21 11:49:06 2009 Subject: [Histonet] Peloris Tissue Processor In-Reply-To: <4B4B66F6AFEB4E518679996481312ED8@wchsys.org> Message-ID: Hello Joyce, Thank you for addressing this. You are correct, it is not recommended to use recycled clearing reagents on the Peloris; recycled alcohol is the only recycled reagent acceptable for use on Peloris. Kindest regards, James Chalmers, MLT (ASCP), MT (HHS) Applications Specialist/Trainer Leica Microsystems - Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 Phone: (847)405-5431 Fax: (847)405-7945 "Joyce Cline" To Sent by: "Histonet" histonet-bounces@ lists.utsouthwest cc ern.edu Subject RE: [Histonet] Peloris Tissue 04/21/2009 11:32 Processor AM You cannot use recycled Formula 83 in the Peloris. The threshold is 88%. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of thisisann@aol.com Sent: Monday, April 20, 2009 10:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peloris Tissue Processor Does anyone use recycled and/or fresh Formula 83 as a clearant on their Peloris Tissue Processor?? If so, can you tell me what final threshold percentage you use. Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From mward <@t> wfubmc.edu Tue Apr 21 11:52:06 2009 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Apr 21 11:52:19 2009 Subject: [Histonet] Beta F1 antibody Message-ID: <61135F0455D33347B5AAE209B903A30429D34DD5@EXCHVS2.medctr.ad.wfubmc.edu> I have had a request for this antibody and have had some difficulty locating a vendor for it. We will be using the Leica Bond Max and if anyone has any tips, vendor, etc. I would appreciate some advice. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Molecular Diagnostics Lab Wake Forest University Baptist Medical Center Winston-Salem, NC From nancy.troiano <@t> yale.edu Tue Apr 21 12:37:01 2009 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Tue Apr 21 12:37:07 2009 Subject: [Histonet] BRDU, Tetracycline, calcein in bone Message-ID: <5.2.1.1.2.20090421132853.0215ce78@email.med.yale.edu> I would recommend fixing the bones in 70% ethanol, embedding in methylmethacrylate undecalcified and cutting 8 - 10 micron thick sections for visualization of tetracycline and/or calcein labels. Decalcification will remove fluorescent labels bound to the mineral in bone as mineral is removed by decal procedures. You may need a larger microtome (Polycut) to cut these bones depending on the size. The fluorescent labels in combination with a nice von Kossa stain would certainly show formation/ mineralization of bone in this area which you can quantify using an Osteomeasure or other system, however, you may want to do a few in paraffin for optimal BRDU label preservation. I would recommend a slow embedding method for these bones - you can check our article in the JOH, June 2004, Vol. 27(2) pp119-130 which describes our slow embedding method for rat bones which can be adapted for rabbit bones. You can contact me for suggestions and with questions if you would like to. From MAUGER <@t> email.chop.edu Tue Apr 21 12:52:10 2009 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Tue Apr 21 12:52:41 2009 Subject: [Histonet] Beta F1 antibody In-Reply-To: <61135F0455D33347B5AAE209B903A30429D34DD5@EXCHVS2.medctr.ad.wfubmc.edu> References: <61135F0455D33347B5AAE209B903A30429D34DD5@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: <49EDCF8A0200003100000EAE@email.chop.edu> Martha, I think you want the TCR-BF1 antibody. It is a T cell recptor for Beta F1. We get it from Pierce (Endogen), cat# TCR1151. We run it on the Bondmax and it works well. Jo Mauger >>> "Martha Ward" 04/21/09 12:52 PM >>> I have had a request for this antibody and have had some difficulty locating a vendor for it. We will be using the Leica Bond Max and if anyone has any tips, vendor, etc. I would appreciate some advice. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Molecular Diagnostics Lab Wake Forest University Baptist Medical Center Winston-Salem, NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lori.garcia <@t> medtronic.com Tue Apr 21 13:11:45 2009 From: lori.garcia <@t> medtronic.com (Garcia, Lori, Sr. Scientist) Date: Tue Apr 21 13:11:50 2009 Subject: [Histonet] Cloudy MMA embedded block In-Reply-To: <698272155.25811240321505599.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <698272155.25811240321505599.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <0F844FC5FD7212428416B8565B47FA0202BF369A@STSM1BMSGM03.ent.core.medtronic.com> In our lab we do store our MMA solutions in a flammable storage refrigerator per EHS requirements; however we put Molecular Sieves in all solutions to keep moisture from contaminating them. We rarely have any problems with cloudy blocks. We order them from VWR, catalog # JT2708-1, 500 g, ~$40 each; manufacturer is J.T. Baker. They last a very long time and are useful for any open non-aqueous solution. Just add a few (6-12) to each container. Hope this helps! Lori -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Tuesday, April 21, 2009 6:45 AM To: Jack Ratliff Cc: Subject: Re: [Histonet] Cloudy MMA embedded block Hi, We don't store our MMA or DBP in the refirgerator as the movement between 4C and room temperature can cause condensation in the bottles.? This just adds water to the material and as it is cooled and re-warmed you are adding more each time and in both directions.?? When it comes to room temperature and then is replaced in the refrigerator it again will develop condensation.? Over time the MMA will have enough water in it to become discolored.? I am surprised you are not seeing the discoloration in the MMA liquid as it poured as a warning not to use it.? We have fair control of temperature in the laboratory and are generally between 68F to 75F with the average as around 70F.? We have never had a problem with the MMA polymerizing or developing a water content.? It is stored in a dark cabinet that is in an area I would say is a little cooler than the room.? We generally don't polymerize in the cold at 4C however, you timing seems long for the process.? It is best not to disturb the vials too much once you are in the embedding phase.? Movement or inversion of the vials can cause a problem as the MMA will polymerize from the bottom up and remixing it can slow the process.? Also make sure you tops are tight at this step. Pam Marcum UPENN Vet Sch New Botlon Center ----- Original Message ----- From: "Jack Ratliff" To: "Ooi Ting Huay" , " " Sent: Tuesday, April 21, 2009 9:24:29 AM GMT -05:00 US/Canada Eastern Subject: Re: [Histonet] Cloudy MMA embedded block Ooi, Somehow you are getting moisture/water mixed in with the MMA solution(s). I only remember visiting the IMCB facility, so if this is not your facility then I am not sure of your laboratory environment (air temperature/humidity). The best thing I can tell you at this point is to try and find ways to reduce the chance for moisture accumulation. I am pretty sure that at a minimum you are storing your catalyst and monomer (MMA) at 4C and your softner (DBP) at room temp. Maybe you should take extra steps to ensure your chemicals are at room temp before any use. Lastly, your polymerization is taking too long for your small 20mL vials. My specimens of similar size require only 3-5 days to completely polymerize. Now, I dont remember your concentration of catalyst, but if it is around 2.5g/1L embedding solution, try skipping the 4C step and use room temp (<23C) until the specimens are polymerized. Then finish them off in the oven when they are tacky or mostly hard on top. The 4C step is mostly to slow the polymerization for larger specimens requiring a larger volume of solution and/or when the lab temp cannot be controlled routinely at or below 23C. The water bath then also helps to regulate the temperature of the polymerization in the case where the lab environment is too cool (<20C) and retarding the rate of polymerzation. The main thing to keep in mind is that the lab environment pretty much controls the process and is probably the main variable that will contrast labs that perform the exact same method. Jack On Apr 21, 2009, at 12:33 AM, ooi.ting.huay@nhc.com.sg wrote: > Hi, > > I am trying very hard to get a clear MMA embedded block. I am ? > appreciate > if there is any advice on doing it. I am using 100% MMA (liquid) and ? > 0.5% > Perkadox 16 (powder) to do the embedding. I have tried to embed the ? > sample > in different conditions. However, it seems to be extremely hard to ? > get a > clear embedded block. > > Below are the methods that I have tried. > > 1. Before embedding, bring the MMA and perkadox 16 to room ? > temperature. > After a few mix it by invertion, I aliquot the solution to the glass ? > vials > that contain samples or simply glass vials that without sample. I ? > put the > glass vials (both with and without sample) in 4 degree, room ? > temperature > and even 37degree oven. > > 2. Mix the MMA and perkadox when it is cold, invert it a few times. > Aliquot it to glass vials that contain sample or without sample. Put ? > in > 4degree, room temperature and 37degree oven. > > I have also tried to speed up the polymerization process by using ? > vacumm > for a few samples... > > Throughout all the sample that I have, I only manage to get a clear ? > empty > glass vial which I put in the 4degree for one month and a clear ? > embedded > sample which I put in 4 degree overnight and bring it out to room > temperature. The rest of the glass vials (with or without sample) are > cloudy. > > I have repeated the methods that I used to get a clear embedded ? > block, I > did not get any clear block (for both with and without sample). > > I am glad if there is any expert advice or guidance. We need a clear > embedded sample. Thank you very much! > > > > Regards, > Ooi > _________________________________________________ > Confidential information. Unauthorized use or disclosure prohibited. > Refer http://www.singhealth.com.sg/ContactUs/#Disclaimer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From robert_schoonhoven <@t> yahoo.com Tue Apr 21 13:20:01 2009 From: robert_schoonhoven <@t> yahoo.com (Robert Schoonhoven) Date: Tue Apr 21 13:20:05 2009 Subject: [Histonet] BRDU, Tetracycline, calcein in bone In-Reply-To: <5.2.1.1.2.20090421132853.0215ce78@email.med.yale.edu> References: <5.2.1.1.2.20090421132853.0215ce78@email.med.yale.edu> Message-ID: <702016.34757.qm@web31103.mail.mud.yahoo.com> You can also perform BrdU on the MMA sections the BrdU label is quite hardy. Robert Schoonhoven ________________________________ From: Nancy W. Troiano To: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 21, 2009 1:37:01 PM Subject: [Histonet] BRDU, Tetracycline, calcein in bone I would recommend fixing the bones in 70% ethanol, embedding in methylmethacrylate undecalcified and cutting 8 - 10 micron thick sections for visualization of tetracycline and/or calcein labels. Decalcification will remove fluorescent labels bound to the mineral in bone as mineral is removed by decal procedures. You may need a larger microtome (Polycut) to cut these bones depending on the size. The fluorescent labels in combination with a nice von Kossa stain would certainly show formation/ mineralization of bone in this area which you can quantify using an Osteomeasure or other system, however, you may want to do a few in paraffin for optimal BRDU label preservation. I would recommend a slow embedding method for these bones - you can check our article in the JOH, June 2004, Vol. 27(2) pp119-130 which describes our slow embedding method for rat bones which can be adapted for rabbit bones. You can contact me for suggestions and with questions if you would like to. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dlschneider <@t> gmail.com Tue Apr 21 13:53:11 2009 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Tue Apr 21 13:53:16 2009 Subject: [Histonet] Reusing specimen containers In-Reply-To: References: Message-ID: <1085e7000904211153x42fa1bb0y3eb1bfb2cb983a1b@mail.gmail.com> I have no idea what CAP or JCAHO says about this but it's a very bad idea. Cross contamination happens. If you reuse containers -- and I don't care what precautions you take -- you will rarely or occasionally fail to adequately clean a container and you will end up mixing patient tissues. The cost of a specimen container is a minute fraction of the cost of processing a specimen. Ask yourself how much money you're really saving. You're scrapping for pennies. Or is this some sort of "green" initiative? In any case, it's asking for problems Daniel Schneider On Tue, Apr 21, 2009 at 11:43 AM, Kelly Colpitts wrote: > > Hi Histoland! > > > > I'm just wondering what folks out there are doing about specimen > containers. Is anyone cleaning them out and reusing them? Is there any CAP > or Joint Commission regulations that say that all specimen containers can > only be used once or can you reuse them as long as they have been thoroughly > cleaned? > > > > Thanks for you all your input, > > Kelly > > _________________________________________________________________ > Windows Live? SkyDrive?: Get 25 GB of free online storage. > > http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_skydrive_042009_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From petepath <@t> yahoo.com Tue Apr 21 17:40:50 2009 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Tue Apr 21 17:40:54 2009 Subject: [Histonet] Reusing specimen containers Message-ID: <600847.9181.qm@web45103.mail.sp1.yahoo.com> I do not know the regulation on this but certainly fixing pans are used repeatedly and probably often without meticulous cleaning. I would venture to ?guess that before there were so many disposables available, reusing containers was common place.? I have to agree with Daniel Schneider. Risk of cross contamination of small biopsy container would not be worth the cost savings.?And if it was cleaned very well what would cost more the container or the person cleaning it? On the other hand?I hate seeing all that plastic in the garbage and ending?up incinerated.?I wonder if it would be legal to autoclave it and recycle it??Is anyone recycling all this plastic? Sounds like a noble business venture. Stephen Peters M.D.? 201 847 0052 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 Phone and fax 201 847 7600 www.pathologyinnovations.com From gmartin <@t> marshallmedical.org Tue Apr 21 18:07:58 2009 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Tue Apr 21 18:08:04 2009 Subject: [Histonet] Reusing specimen containers In-Reply-To: <1085e7000904211153x42fa1bb0y3eb1bfb2cb983a1b@mail.gmail.com> References: <1085e7000904211153x42fa1bb0y3eb1bfb2cb983a1b@mail.gmail.com> Message-ID: <6ED9D4252F278841A0593D3D788AF24C0513A540@mailsvr.MARSHMED.local> We cleaned bottles everyday for years and never had a problem ... having said that ... it not a good idea in the end because of the time and the exposure to the formalin. Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider Sent: Tuesday, April 21, 2009 11:53 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Reusing specimen containers I have no idea what CAP or JCAHO says about this but it's a very bad idea. Cross contamination happens. If you reuse containers -- and I don't care what precautions you take -- you will rarely or occasionally fail to adequately clean a container and you will end up mixing patient tissues. The cost of a specimen container is a minute fraction of the cost of processing a specimen. Ask yourself how much money you're really saving. You're scrapping for pennies. Or is this some sort of "green" initiative? In any case, it's asking for problems Daniel Schneider On Tue, Apr 21, 2009 at 11:43 AM, Kelly Colpitts wrote: > > Hi Histoland! > > > > I'm just wondering what folks out there are doing about specimen > containers. Is anyone cleaning them out and reusing them? Is there any CAP > or Joint Commission regulations that say that all specimen containers can > only be used once or can you reuse them as long as they have been thoroughly > cleaned? > > > > Thanks for you all your input, > > Kelly > > _________________________________________________________________ > Windows Live(tm) SkyDrive(tm): Get 25 GB of free online storage. > > http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_skydrive_042009 _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nefff <@t> staff.uni-marburg.de Wed Apr 22 01:58:23 2009 From: nefff <@t> staff.uni-marburg.de (Dr. Frauke Neff) Date: Wed Apr 22 01:58:35 2009 Subject: [Histonet] Reusing specimen containers In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C0513A540@mailsvr.MARSHMED.local> References: <1085e7000904211153x42fa1bb0y3eb1bfb2cb983a1b@mail.gmail.com> <6ED9D4252F278841A0593D3D788AF24C0513A540@mailsvr.MARSHMED.local> Message-ID: <20090422085823.idlnm1w54wkgogsg@home.staff.uni-marburg.de> We did it like Gary wrote: We had an extra "dish washer" for the containers, that produce a nice "melange" of formalin, damp and desinfectant. Cross contamination did not occur by washing the containers but at the point of gross section or even at the OR itself.... Frauke Zitat von "Martin, Gary" : > We cleaned bottles everyday for years and never had a problem ... having > said that ... it not a good idea in the end because of the time and the > exposure to the formalin. > Gary > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel > Schneider > Sent: Tuesday, April 21, 2009 11:53 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Reusing specimen containers > > I have no idea what CAP or JCAHO says about this but it's a very bad > idea. > Cross contamination happens. If you reuse containers -- and I don't > care > what precautions you take -- you will rarely or occasionally fail to > adequately clean a container and you will end up mixing patient tissues. > > The cost of a specimen container is a minute fraction of the cost of > processing a specimen. Ask yourself how much money you're really > saving. > You're scrapping for pennies. Or is this some sort of "green" > initiative? > In any case, it's asking for problems > > Daniel Schneider > > > > > > On Tue, Apr 21, 2009 at 11:43 AM, Kelly Colpitts > > wrote: > >> >> Hi Histoland! >> >> >> >> I'm just wondering what folks out there are doing about specimen >> containers. Is anyone cleaning them out and reusing them? Is there > any CAP >> or Joint Commission regulations that say that all specimen containers > can >> only be used once or can you reuse them as long as they have been > thoroughly >> cleaned? >> >> >> >> Thanks for you all your input, >> >> Kelly >> >> _________________________________________________________________ >> Windows Live(tm) SkyDrive(tm): Get 25 GB of free online storage. >> >> > http://windowslive.com/online/skydrive?ocid=TXT_TAGLM_WL_skydrive_042009 > _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From ree3 <@t> leicester.ac.uk Wed Apr 22 03:15:08 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed Apr 22 03:15:37 2009 Subject: [Histonet] haemotol autostainer? Message-ID: <7722595275A4DD4FA225B92CDBF174A17457783D1A@EXC-MBX3.cfs.le.ac.uk> A colleague is looking to purchase a staining machine, primarily for Romanowsky type staining, any recommendations or brick bats please. Richard Edwards Leicester University...UK. From Imcgonnell <@t> RVC.AC.UK Wed Apr 22 09:20:22 2009 From: Imcgonnell <@t> RVC.AC.UK (McGonnell, Imelda Mary) Date: Wed Apr 22 09:20:29 2009 Subject: [Histonet] tissue non specific alkaline phosphatase antibodies Message-ID: <193A454735ABFE438D08FA139B54B005058AA288@cmw2kex01.rvc.ac.uk> Hi, I want to find an alkaline phosphatase antibody that will label bone in sections of chick embryonic tissue. Does anyone know if such an antibody exists? Many thanks for your help Imelda Dr Imelda McGonnell Lecturer Dept. Veterinary Basic Sciences Royal Veterinary College Royal College St London NW1 0TU Phone : Extn. 5453 Direct dial 020 7468 1223 From asachau <@t> titanmed.com Wed Apr 22 10:59:48 2009 From: asachau <@t> titanmed.com (April Sachau) Date: Wed Apr 22 11:01:30 2009 Subject: [Histonet] Mohs Histology position!!! In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390872FAF961@IBMB7Exchange.digestivespecialists.com> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F701E67C03@titansbs1.corp.titanmed.com> Hello Histo-land! I have a temporary Mohs Histology position available at this time. Dayshift, Monday through Friday. Please let me know if you are interested or know someone seeking a temporary position at this time. Thanks!!! :) April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From mabosso <@t> unipathllc.com Wed Apr 22 12:30:24 2009 From: mabosso <@t> unipathllc.com (Mary Abosso) Date: Wed Apr 22 12:34:53 2009 Subject: [Histonet] CAP question Message-ID: <43A451981FF6634795BE83B1B5494D631BDF8C@exchange.unipathllc.corp> Hi all, I am updating procedures and am wondering if there is a better format for compliance with ANP.22250 - Does the procedure manual address all methods and antibodies currently in use? Just wondering if I need to include every antibody or a generic procedure covering most antibodies, with the exceptions only reference within the same procedure? Any help would be appreciated. Thanks, Mary Abosso From j.aflleje <@t> integrated-pathology.com Wed Apr 22 12:48:46 2009 From: j.aflleje <@t> integrated-pathology.com (James Aflleje) Date: Wed Apr 22 12:48:51 2009 Subject: [Histonet] Hello Message-ID: <1BEBBEAB73EB8F40B2CEF99ACD5B33A7AA6757B0E3@Exchange.IPath.local> Hello fellow histologists from Phoenix, Arizona. I am fairly new to posting on this histonet site; although, I have used this site for references and troubleshooting. I am wondering if anyone knows anybody that would like an opportunity for a full time position as a histologist for a small anatomical pathology reference laboratory in the west phoenix area in Arizona. From portera <@t> msu.edu Wed Apr 22 12:55:45 2009 From: portera <@t> msu.edu (Amy Porter) Date: Wed Apr 22 12:55:45 2009 Subject: [Histonet] CAP question References: <43A451981FF6634795BE83B1B5494D631BDF8C@exchange.unipathllc.corp> Message-ID: Mary - I have one generic protocol with a spreadsheet attached stating the following: Name of Antibody CD/CK # Clone Species Pretreatment Dilution Incubation time Enzyme (ABC or AP) Lot # in Use Hope this helps out it has passed in the past. take care. amy Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Mary Abosso" To: "Histonet" Sent: Wednesday, April 22, 2009 1:30 PM Subject: [Histonet] CAP question Hi all, I am updating procedures and am wondering if there is a better format for compliance with ANP.22250 - Does the procedure manual address all methods and antibodies currently in use? Just wondering if I need to include every antibody or a generic procedure covering most antibodies, with the exceptions only reference within the same procedure? Any help would be appreciated. Thanks, Mary Abosso _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pkarlisch <@t> hmc.psu.edu Wed Apr 22 12:51:06 2009 From: pkarlisch <@t> hmc.psu.edu (Patricia Karlisch) Date: Wed Apr 22 12:58:23 2009 Subject: [Histonet] RE: Reusing Specimen Containers Message-ID: <49EF2258.07B7.008C.0@hmc.psu.edu> I think reusing a specimen container is not a good idea. It only takes one incident of cross contamination to bring a potential lawsuit. However, what about labeling the containers. Do you have to peel all the labels off, including the formalin chemical hazard label? I believe too that the tech time is much more costly and the exposure to formalin is unnecessary. Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From rjbuesa <@t> yahoo.com Wed Apr 22 13:39:14 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 22 13:39:18 2009 Subject: [Histonet] CAP question In-Reply-To: <43A451981FF6634795BE83B1B5494D631BDF8C@exchange.unipathllc.corp> Message-ID: <675341.86508.qm@web65713.mail.ac4.yahoo.com> I used to have a generic protocol for each type of procedure. For example: the lectin procedure for Ulex europaeus was summarized apart for the general used with most of the Abs using HIER. There was also a general protocol when the procedure was completed manually of with the Dako autostainer that we used.The procedure for Her2-neu was also apart. Also I had a spread sheet for the different antibodies in use and for each it was stated the HIER pH required?and other relevant data for the antigen, as well as the type of control used. Ren? J. --- On Wed, 4/22/09, Mary Abosso wrote: From: Mary Abosso Subject: [Histonet] CAP question To: "Histonet" Date: Wednesday, April 22, 2009, 1:30 PM Hi all, I am updating procedures and am wondering if there is a better format for compliance with ANP.22250 - Does the procedure manual address all methods and antibodies currently in use? Just wondering if I need to include every antibody or a generic procedure covering most antibodies, with the exceptions only reference within the same procedure? Any help would be appreciated. Thanks, Mary Abosso _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Wed Apr 22 14:08:39 2009 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Wed Apr 22 14:09:52 2009 Subject: [Histonet] Myeloperoxidase Antibody Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BAEBE18E0E@NADCWPMSGCMS03.hca.corpad.net> Good Afternoon Everyone, Where do you recommend ordering the pre-dilute antibody Myeloperoxidase MPO Ab-2? Biocare and Lab Vision have both discontinued carrying it. Please help!!!!! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax From LSebree <@t> uwhealth.org Wed Apr 22 14:34:16 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Apr 22 14:34:49 2009 Subject: [Histonet] Myeloperoxidase Antibody In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BAEBE18E0E@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A5892CDF8F@UWHC-MAIL01.uwhis.hosp.wisc.edu> I don't know if I wholeheartedly recommend it but we switched to Cell Marque. The reason for my qualifying statement is because we went from not needing any HIER to having to use a pretty harsh retrieval. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Wednesday, April 22, 2009 2:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Myeloperoxidase Antibody Good Afternoon Everyone, Where do you recommend ordering the pre-dilute antibody Myeloperoxidase MPO Ab-2? Biocare and Lab Vision have both discontinued carrying it. Please help!!!!! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From drvet_anjan <@t> hotmail.com Wed Apr 22 15:41:36 2009 From: drvet_anjan <@t> hotmail.com (anjan kumar) Date: Wed Apr 22 15:41:40 2009 Subject: [Histonet] levamisole Message-ID: hi everyone, can anyone tell me how to prepare 125mM of levamisole. Dr. Anjan Kumar.K.R M.V.Sc Scholar Dept. of Veterinary Pathology Madras Veterinary College Chennai-7 India email: drvet_anjan@hotmail.com Phone: +91-9940475801 _________________________________________________________________ How fun is this? IMing with Windows Live Messenger just got better. http://www.microsoft.com/india/windows/windowslive/messenger.aspx From mari.ann.mailhiot <@t> leica-microsystems.com Wed Apr 22 16:02:59 2009 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Wed Apr 22 16:03:07 2009 Subject: [Histonet] Replacement glass plates for Reichert Microtome Knife Sharpener 903? In-Reply-To: <902069.70864.qm@web110214.mail.gq1.yahoo.com> Message-ID: Yvan Unfortunetly we don't have any plates that will fit the AO903 and the AO935 knife sharpener. The SP9000 plates can only be used on the SP9000. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com yvan lindekens To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Replacement glass plates for Reichert Microtome Knife 04/21/2009 05:28 Sharpener 903? AM Hi all, It struck me that all those microtome knife sharpeners (AO 935, Reichert 903, AO 903...) all look very much the same. Are the glass plates interchangeable? Would the glass plates for the current Leica sharpener SP9000 (part.no. 14041819698, dimensions 292mm x 127mm x 6 mm) fit the Reichert Microtome Knife Sharpener 903? Thanks in advance for your answers! Yvan. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From JWeems <@t> sjha.org Wed Apr 22 17:25:25 2009 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Apr 22 17:25:30 2009 Subject: [Histonet] Elizabeth Sheppard - are you there? Message-ID: <5D64396A0D4A5346BEBC759022AAEAA54C2CAA@ITSSSXM01V6.one.ads.che.org> Would you please contact me? Have some questions about molecular test billing. Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From cfarish <@t> csu.edu.au Wed Apr 22 18:14:06 2009 From: cfarish <@t> csu.edu.au (Farish, Craig) Date: Wed Apr 22 18:14:17 2009 Subject: [Histonet] Finding iron in decalcified sections Message-ID: <79A000B60AFE8045BA2581119DFEC444056254C6@ESWW01.CSUMain.csu.edu.au> Hi folks - I'd very much appreciate some help with this problem. I've been asked to performing the routine histo for a trial investigating the ability of chickens to sense direction. They are believed to have areas of iron rich tissue in their beaks which can detect the magnetic field of the earth in the same fashion as homing pigeons. The problem is this - to section the beaks I need to decalcify them, however I'm pretty sure that standard decalcification removes iron, at least partly. I know that the levels of iron in the tissue are very low and I've just trialled my technique on a pigeon with no success. I'm using a standard Perl stain (2% HCl + 2% K Ferrocyanide for 20 mins) on FFPE tissue which usually works very well for me, fixation is for a minimum of 24 hours in either 10%NBF or Bouins and decalcification is in either 5% Nitric acid or formic acid. Would anyone have any suggestions as to how to decal without removing iron or another stain which I could use in this situation? Thanks in advance for any and all suggestions, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga, Australia c farish@csu.edu.au ' I don't want to achieve immortality through my work, I want to achieve immortality through not dying' - Woody Allen From lpwenk <@t> sbcglobal.net Thu Apr 23 04:50:53 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Apr 23 04:51:05 2009 Subject: [Histonet] Finding iron in decalcified sections In-Reply-To: <79A000B60AFE8045BA2581119DFEC444056254C6@ESWW01.CSUMain.csu.edu.au> Message-ID: <840BC78E3140424BA08FA8C287F9F4EA@HPPav2> Acid decalcifiers will remove some to all iron. Don't use Bouins - picric ACID and acetic ACID will start the decalicification process, and will definitely remove small amounts of iron. Use the NBF. Can you switch to a milder decalcifier for a longer time? - FASC = formic acid-sodium citrate - EDTA The Bancroft book has the procedures for these. If you need them, let me know. Now, with that said, I have a question - what type of iron is it? I know the Prussian Blue iron stain will demonstrate hemosiderin, with ferric ions (FE +3) in the center. And that hemosiderin also contains ferritin, which is a ferric ion (FE +3) protein complex. Prussian blue will demonstrate ferric chloride (Fe +3 ions) when put in goblet cells for the Colloidal iron stain. But Prussian blue will not stain the iron bound in RBC in the form of hemoglobin. That iron is bound to oxygen and proteins, and isn't available for Prussian blue staining. So, I don't happen to know (and would love to learn) what type of iron is in chicken beaks. Is it iron metal filings (no charge, and I'm guessing probably won't stain with Prussian blue), or iron ions but bound to proteins and/or other chemicals that won't stain with Prussian blue? Or is it the type of ferric ion that is available for Prussian blue staining? Or is there another technique that's going to be used to demonstrate the iron? (I love HistoNet. Get to learn new things every day!) Happy National Medical Laboratory Professionals Week! Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48037 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Farish, Craig Sent: Wednesday, April 22, 2009 7:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Finding iron in decalcified sections Hi folks - I'd very much appreciate some help with this problem. I've been asked to performing the routine histo for a trial investigating the ability of chickens to sense direction. They are believed to have areas of iron rich tissue in their beaks which can detect the magnetic field of the earth in the same fashion as homing pigeons. The problem is this - to section the beaks I need to decalcify them, however I'm pretty sure that standard decalcification removes iron, at least partly. I know that the levels of iron in the tissue are very low and I've just trialled my technique on a pigeon with no success. I'm using a standard Perl stain (2% HCl + 2% K Ferrocyanide for 20 mins) on FFPE tissue which usually works very well for me, fixation is for a minimum of 24 hours in either 10%NBF or Bouins and decalcification is in either 5% Nitric acid or formic acid. Would anyone have any suggestions as to how to decal without removing iron or another stain which I could use in this situation? Thanks in advance for any and all suggestions, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga, Australia c farish@csu.edu.au ' I don't want to achieve immortality through my work, I want to achieve immortality through not dying' - Woody Allen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Thu Apr 23 07:12:11 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Apr 23 07:15:03 2009 Subject: [Histonet] Buffer. Message-ID: <417448B4823B47FBBFE2A3658519BDB3@IBLS.GLA.AC.UK> At the moment I'm doing a large batch of myosin ATPase staining on human skeletal muscle. 9.4 and the reversal at 4.3 are excellent but the 4.6 is not so good. I will alter the reversal pH in a range from 4.5 to 4.7 but I'm wondering, does the buffer have an effect. I use a glycine buffer and have done for many years with good results but this time, mmm, not so good. Others have used acetate or barbitone and I have myself but always found glycine to give clean staining after the sulphide rinse. Comments, stick to glycine but alter the reversal pH, or change the whole system and try another buffer? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From rjbuesa <@t> yahoo.com Thu Apr 23 08:20:11 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 23 08:20:17 2009 Subject: [Histonet] Finding iron in decalcified sections Message-ID: <15904.85321.qm@web65713.mail.ac4.yahoo.com> The mandible and maxilla of chicken's' beaks are porous bone covered by horny keratin so it is very likely that you will have to do a double procedure. First to soften the covering keratin with 10% sodium or potasium hydroxide aq. solution and later decalcify the bone. Since it is a poroues bone? you will be able to decalcify it with EDTA, as is done with bone marrow core biopsies, and if you do it that way, the iron will not be removed, as it is not removed from the core BM biopsies. Ren? J. ? ? ? --- On Wed, 4/22/09, Farish, Craig wrote: From: Farish, Craig Subject: [Histonet] Finding iron in decalcified sections To: histonet@lists.utsouthwestern.edu Date: Wednesday, April 22, 2009, 7:14 PM Hi folks - I'd very much appreciate some help with this problem. I've been asked to performing the routine histo for a trial investigating the ability of chickens to sense direction. They are believed to have areas of iron rich tissue in their beaks which can detect the magnetic field of the earth in the same fashion as homing pigeons. The problem is this - to section the beaks I need to decalcify them, however I'm pretty sure that standard decalcification removes iron, at least partly. I know that the levels of iron in the tissue are very low and I've just trialled my technique on a pigeon with no success. I'm using a standard Perl stain (2% HCl + 2% K Ferrocyanide for 20 mins) on FFPE tissue which usually works very well for me, fixation is for a minimum of 24 hours in either 10%NBF or Bouins and decalcification is in either 5% Nitric acid or formic acid. Would anyone have any suggestions as to how to decal without removing iron or another stain which I could use in this situation? Thanks in advance for any and all suggestions, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga, Australia c farish@csu.edu.au ' I don't want to achieve immortality through my work, I want to achieve immortality through not dying' - Woody Allen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Apr 23 08:20:11 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 23 08:22:01 2009 Subject: [Histonet] Finding iron in decalcified sections Message-ID: <289538.86885.qm@web65716.mail.ac4.yahoo.com> The mandible and maxilla of chicken's' beaks are porous bone covered by horny keratin so it is very likely that you will have to do a double procedure. First to soften the covering keratin with 10% sodium or potasium hydroxide aq. solution and later decalcify the bone. Since it is a poroues bone? you will be able to decalcify it with EDTA, as is done with bone marrow core biopsies, and if you do it that way, the iron will not be removed, as it is not removed from the core BM biopsies. Ren? J. ? ? ? --- On Wed, 4/22/09, Farish, Craig wrote: From: Farish, Craig Subject: [Histonet] Finding iron in decalcified sections To: histonet@lists.utsouthwestern.edu Date: Wednesday, April 22, 2009, 7:14 PM Hi folks - I'd very much appreciate some help with this problem. I've been asked to performing the routine histo for a trial investigating the ability of chickens to sense direction. They are believed to have areas of iron rich tissue in their beaks which can detect the magnetic field of the earth in the same fashion as homing pigeons. The problem is this - to section the beaks I need to decalcify them, however I'm pretty sure that standard decalcification removes iron, at least partly. I know that the levels of iron in the tissue are very low and I've just trialled my technique on a pigeon with no success. I'm using a standard Perl stain (2% HCl + 2% K Ferrocyanide for 20 mins) on FFPE tissue which usually works very well for me, fixation is for a minimum of 24 hours in either 10%NBF or Bouins and decalcification is in either 5% Nitric acid or formic acid. Would anyone have any suggestions as to how to decal without removing iron or another stain which I could use in this situation? Thanks in advance for any and all suggestions, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga, Australia c farish@csu.edu.au ' I don't want to achieve immortality through my work, I want to achieve immortality through not dying' - Woody Allen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Thu Apr 23 08:24:25 2009 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Apr 23 08:24:34 2009 Subject: [Histonet] Happy National Medical Laboratory Professionals Week 4/20-4/25 2009 Message-ID: Hi Histonetters! I hope you are doing well. I wanted to send a special bulletin wishing you a Happy National Medical Laboratory Professionals Week. I hope your lab has been having some fun, informative and special events to celebrate because you deserve it. I have heard of some really cool things that labs are doing to mark the occasion including luncheons, costume, trivia and photo contests and displays to educate others about what you do. If you happen to be on Facebook be sure and check out the ASCP and Histology groups there and the event created by the ASCP for National Medical Laboratory Professionals Week. Also I would love to hear what you are doing in your lab to celebrate so please shoot me back an e-mail and let me know. I also wanted to let you know about the great job opportunities I am currently working on. I have supervisor and lead positions in Los Angeles, California, Bedford and Springfield, Massachusetts. I have histotechnician/histotechnologist positions in Los Angeles, CA, Waco, TX, Atlanta, GA, Cape Cod, MA and New York City, NY. I also have a Flow Cytometry position in South Florida. All of my positions are full time permanent positions on the dayshift except for the one in NYC. My clients offer excellent compensation, benefits and relocation assistance. If you or anyone you know might be interested please contact me. I can be reached toll free at 866-607-3542 or at relia1@earthlink.net Thanks-Pam Thank You! Pam Barker President RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free: (866)607-3542 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia From contact <@t> excaliburpathology.com Thu Apr 23 08:30:21 2009 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Apr 23 08:30:26 2009 Subject: [Histonet] Finding iron in decalcified sections Message-ID: <560531.76851.qm@web1115.biz.mail.sk1.yahoo.com> Another thought about iron staining. It may be ferrous iron instead of ferric in the beaks. Prussian blue staining uses potassium ferrocyanide, you might also try using potassium ferricyanide for Turnbull's staining. Paula From PMonfils <@t> Lifespan.org Thu Apr 23 09:16:16 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Apr 23 09:16:41 2009 Subject: [Histonet] Finding iron in decalcified sections In-Reply-To: <79A000B60AFE8045BA2581119DFEC444056254C6@ESWW01.CSUMain.csu.edu.au> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CB8@LSRIEXCH1.lsmaster.lifespan.org> The other approach of course would be to avoid decalcification altogether and embed them in methacrylate, provided you are set up to embed and section such blocks. From estellamireles <@t> gmail.com Thu Apr 23 10:55:46 2009 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Thu Apr 23 10:55:52 2009 Subject: [Histonet] EM Knife Message-ID: All EM Techs. Which Ultra knife is better. Diatome or Pelco? Is there any other company that I should consider. I plan to purchase an Ultra and a Histo knife. Thank You Stella From rjbuesa <@t> yahoo.com Thu Apr 23 10:59:14 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 23 10:59:19 2009 Subject: [Histonet] EM Knife In-Reply-To: Message-ID: <720992.28181.qm@web65705.mail.ac4.yahoo.com> We always used Diatome and they also took care of "resharpening" our diamond knifes. Always satisfied with their service. Ren? J. --- On Thu, 4/23/09, Stella Mireles wrote: From: Stella Mireles Subject: [Histonet] EM Knife To: Histonet@lists.utsouthwestern.edu Date: Thursday, April 23, 2009, 11:55 AM All EM Techs. Which Ultra knife is better. Diatome or Pelco? Is there any other company that I should consider. I plan to purchase an Ultra and a Histo knife. Thank You Stella _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Thu Apr 23 10:58:45 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Apr 23 10:59:56 2009 Subject: [Histonet] Buffer. In-Reply-To: <3CE20ED86C4A114EBDF3BCE8DEFD8F60D6634F@msxc06.OSUMC.EDU> References: <417448B4823B47FBBFE2A3658519BDB3@IBLS.GLA.AC.UK> <3CE20ED86C4A114EBDF3BCE8DEFD8F60D6634F@msxc06.OSUMC.EDU> Message-ID: Bonnie, You hate ATPase, meet your friend, bane of my life, one of the most capricious techniques ever to punish a poor Histotech. Although I'm doing a large number of subjects I keep the numbers down and like your friend 8-9 slides per batch. 9.4 and 4.3 are always ok it's the 4.6 that gives the grief. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: Whitaker, Bonnie [mailto:Bonnie.Whitaker@osumc.edu] Sent: 23 April 2009 16:33 To: ian.montgomery@bio.gla.ac.uk Subject: RE: [Histonet] Buffer. Ian, I hate ATPase, but when I was at University of TX, we did them. One of my techs noticed that she did not have as good luck with large batches. I can't really say if this was the case, but she swore they were much better if she broke them down into smaller batches. I think she didn't like to do more than 8-9 slides per batch. Good Luck!! Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 Bonnie.Whitaker@osumc.edu phone 614.293.5048 fax 614.293.7273 pager 614.346.5013 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Thursday, April 23, 2009 8:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Buffer. At the moment I'm doing a large batch of myosin ATPase staining on human skeletal muscle. 9.4 and the reversal at 4.3 are excellent but the 4.6 is not so good. I will alter the reversal pH in a range from 4.5 to 4.7 but I'm wondering, does the buffer have an effect. I use a glycine buffer and have done for many years with good results but this time, mmm, not so good. Others have used acetate or barbitone and I have myself but always found glycine to give clean staining after the sulphide rinse. Comments, stick to glycine but alter the reversal pH, or change the whole system and try another buffer? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jessgrocki <@t> yahoo.com Thu Apr 23 11:09:02 2009 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Thu Apr 23 11:09:05 2009 Subject: [Histonet] Negative Tissue Controls for IHC Validations Message-ID: <844248.45763.qm@web82005.mail.mud.yahoo.com> Hi Everyone, We are having a hard time figuring out what tissue to use in testing for specificity and sensitivity for the following antibodies: CDX-2 Chromogranin A HCG NSE Synaptophysin H.pylori Factor XIIIa CK 5/6 If anyone wouldn't mind sharing with us what they know we would really appreciate it!! Thanks! ? Jessica Piche-Grocki, HT(ASCP) From Margaret.Perry <@t> sdstate.edu Thu Apr 23 11:53:28 2009 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Apr 23 11:53:42 2009 Subject: [Histonet] ground meat samples Message-ID: We will be doing IHC on ground pork and would like tips on how to process and embed the samples. Can you leave the processed sample in a paper biopsy bag and embed both the meat and the paper? Will cutting the paper make the blade dull to quickly? Is there anything else we can use to hold the tissue together? We had thought to maybe use pig intestine and pack the meat into it to make it cut better. Thank you in advance for your help. Histonet has helped me out many times and I greatly appreciate it. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From rcharles <@t> state.pa.us Thu Apr 23 12:20:37 2009 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Thu Apr 23 12:20:44 2009 Subject: [Histonet] RE: ground meat samples In-Reply-To: Message-ID: <3809C163DC1DA54AA534B3C7794D07B632A1C80A45@ENHBGMBX01.PA.LCL> Sorry Histonetter's But it is my Friday and wouldn't this method give new meaning to the term "sausage block." :) I wish Margaret all the luck in her quest and if it was me, I would try either or both methods. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rcharles@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Thursday, April 23, 2009 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ground meat samples We will be doing IHC on ground pork and would like tips on how to process and embed the samples. Can you leave the processed sample in a paper biopsy bag and embed both the meat and the paper? Will cutting the paper make the blade dull to quickly? Is there anything else we can use to hold the tissue together? We had thought to maybe use pig intestine and pack the meat into it to make it cut better. Thank you in advance for your help. Histonet has helped me out many times and I greatly appreciate it. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Thu Apr 23 12:48:17 2009 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Apr 23 12:48:24 2009 Subject: [Histonet] RE: ground meat samples In-Reply-To: <3809C163DC1DA54AA534B3C7794D07B632A1C80A45@ENHBGMBX01.PA.LCL> References: <3809C163DC1DA54AA534B3C7794D07B632A1C80A45@ENHBGMBX01.PA.LCL> Message-ID: <75A0543E23D3A7458012D9E02EDBEC0002E9248A46@UTHCMS1.uthouston.edu> Roger If I were you I would compress this into a block before fixation. Can use paper up to wax but would not use paper covering in the embedded block it will make cutting too difficult. Alternative is to use sausage skin as the covering and process, embed and cut with this. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Thursday, April 23, 2009 12:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: ground meat samples Sorry Histonetter's But it is my Friday and wouldn't this method give new meaning to the term "sausage block." :) I wish Margaret all the luck in her quest and if it was me, I would try either or both methods. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rcharles@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Thursday, April 23, 2009 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ground meat samples We will be doing IHC on ground pork and would like tips on how to process and embed the samples. Can you leave the processed sample in a paper biopsy bag and embed both the meat and the paper? Will cutting the paper make the blade dull to quickly? Is there anything else we can use to hold the tissue together? We had thought to maybe use pig intestine and pack the meat into it to make it cut better. Thank you in advance for your help. Histonet has helped me out many times and I greatly appreciate it. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Thu Apr 23 12:50:06 2009 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Apr 23 12:50:11 2009 Subject: [Histonet] Ground meat processing Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E682B@nmdamailsvr.nmda.ad.nmsu.edu> What would be wrong with just submitting the ground sample as one would with a curetting or similar - that is if the pieces are small, submit them for processing in a lens paper packet or one of those paper histo bags, then scrape the pieces off the paper and embed into paraffin as usual? Perhaps I'm thrown off a bit by the part about embedding the paper along with the tissue? Do you have some special requirement for processing for IHC that makes it necessary to consider embedding the paper? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Shakun.Aswani <@t> acologix.com Thu Apr 23 12:52:15 2009 From: Shakun.Aswani <@t> acologix.com (Shakun Aswani) Date: Thu Apr 23 12:52:21 2009 Subject: [Histonet] levamisole In-Reply-To: References: Message-ID: <777AB0DE519C8E46A6220E2287C5BAD301B34E9E@EXCHANGE.acologix.com> Hi Anjan, Here is the molecular weight of the levamisole, from this you can calculate the 125mM Levamisole Analytical Information * Synonym: leva (l)-Tetramisole * Chemical Name: (6S)-2,3,5,6-Tetrahydro-6-phenylimidazo[2,1-b]thiazole * Chemical Formula: C11H12N2S*HCl * Molecular Weight: 204.3 * Formula Weight: 240.8 * pKa: 8.0 * Levamisole is extracted using a basic liquid-liquid chlorobutane extraction with an acid back extraction. * Levamisole is easily detected on the GC/NPD and GC/MS. * Elution Order: Doxylamine, LEVAMISOLE, Chlorpheniramine I hope this will help you Regards, Shakun Note: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anjan kumar Sent: Wednesday, April 22, 2009 1:42 PM To: triple immunohistochem Subject: [Histonet] levamisole hi everyone, can anyone tell me how to prepare 125mM of levamisole. Dr. Anjan Kumar.K.R M.V.Sc Scholar Dept. of Veterinary Pathology Madras Veterinary College Chennai-7 India email: drvet_anjan@hotmail.com Phone: +91-9940475801 _________________________________________________________________ How fun is this? IMing with Windows Live Messenger just got better. http://www.microsoft.com/india/windows/windowslive/messenger.aspx_______ ________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Thu Apr 23 12:56:57 2009 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Apr 23 12:57:54 2009 Subject: [Histonet] EM Knife In-Reply-To: References: Message-ID: <49F0ABE9.5050704@umdnj.edu> Hi Stella: All of our knives are Diatome and I have no complaints. On the other hand, I have been doing business with Pelco for many years with no problems ever. It seems unlilely that they are making their own knives so they are probably buying and rebranding. You will probably be fine with either. Geoff Stella Mireles wrote: > All EM Techs. Which Ultra knife is better. Diatome or Pelco? Is there any > other company that I should consider. I plan to purchase an Ultra and a > Histo knife. > Thank You > Stella > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From MadaryJ <@t> MedImmune.com Thu Apr 23 13:08:05 2009 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Thu Apr 23 13:08:14 2009 Subject: [Histonet] new vs used microtomes(regular rotary) Message-ID: I bought a couple of Microms and so far they have been great. Wondering if anyone has purchased a new rotary microtome recently for a decent price that they like. I have bought a couple of used as well many years ago from a guy I trusted no longer in the business. Any suggestions there? I have purchased used equipment from IMEB and BelAir both have been fine. Just checking my options here. I have an RMS that I never really bothered using that has electronic advance/retract that works fine but I am a little old school and that little feature is more annoying where some folks love it. If there are any vendors out there who deal in used equipment and would give me something on a trade in, that might make me consider you over someone else. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From schaundrawalton <@t> yahoo.com Thu Apr 23 13:25:22 2009 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Thu Apr 23 13:25:25 2009 Subject: [Histonet] Grossing Message-ID: <435583.27358.qm@web58908.mail.re1.yahoo.com> We are evaluating some of our grossing procedures and cut off times and I was wondering what other people are doing. ? Who does your grossing, pathologist, PA, or other qualified individuals? Who does you accessioning, histotechs, support staff, or other? What is your cut off time for grossing tissues? ? Right now our pathologists gross all tissues, the histotechs accession, and grossing is done up until 6pm (which is when the processor begins). ? Thanks for the info! ? -Schaundra Walton HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL From Jennifer.Bull <@t> northwestpathology.com Thu Apr 23 13:27:07 2009 From: Jennifer.Bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Thu Apr 23 13:27:12 2009 Subject: [Histonet] Fatty Breast Tissue processing Message-ID: <85760CECEC18444BB95F26D5E88DAEAA223F261C25@hinet2.hinet.org> Is anyone willing to share their protocol for processing fatty breast tissue? It is difficult to find any documentation or processes that work really well. Thank you in advance, you have all been extremely helpful to me! Jennifer Bull Histology Supervisor jennifer.bull@nwpathology.com From marktarango <@t> gmail.com Thu Apr 23 13:36:23 2009 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Apr 23 13:36:27 2009 Subject: [Histonet] Herpes Message-ID: <5b6eb13e0904231136j4bccbb31i55c757c86e053378@mail.gmail.com> Does anyone have a herpes control for which they'd be willing to trade? I can trade just about any tumor or control material you might need.... thanks Mark Tarango From Dolores_Fischer <@t> baxter.com Thu Apr 23 13:37:01 2009 From: Dolores_Fischer <@t> baxter.com (Dolores_Fischer@baxter.com) Date: Thu Apr 23 13:37:09 2009 Subject: [Histonet] ground meat processing Message-ID: I agree with Sally, why not just process as a curretting by loosely placing pieces of ground meat in a cassette and embed in an appropriate sized mold for your IHC needs. Paper will shred, it does not section well, if at all. Dolores Fischer The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From Steven.Joy <@t> capitalhealth.ca Thu Apr 23 13:38:58 2009 From: Steven.Joy <@t> capitalhealth.ca (Steven Joy) Date: Thu Apr 23 13:39:03 2009 Subject: [Histonet] commercial control slides Message-ID: <1101A09566B25D4BB3889A44FDC1E7040A9B18@uantexchg03.capitalhealth.ca> Can anyone recommend a brand or line of commercial control slides for oil red O? Thanks, Steve Joy, BSc. MLT Research and Development Technologist 5B2.03 Anatomical Pathology University of Alberta Hospital 8440-112 st Edmonton Ab T6G 2B7 Phone: (780) 407-8015 Fax: (780) 407-3009 Email: steven.joy@capitalhealth.ca This communication is intended for the sole use of the recipient to which it was addressed and may contain confidential, personal or privileged information. Please contact the sender immediately if you are not the intended recipient of this information and do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. Thank you. From tifei <@t> foxmail.com Thu Apr 23 13:44:12 2009 From: tifei <@t> foxmail.com (TF) Date: Thu Apr 23 13:44:52 2009 Subject: [Histonet] Myeloperoxidase Antibody References: <9E2D36CE2D7CBA4A94D9B22E8328A3BAEBE18E0E@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <200904240244042496604@foxmail.com> SGksIHdlIHVzZSANClJiIGEgSHUgTXllbG9wZXJveGlkYXNlICANCkRha29jeXRvbWF0IA0KQTAz OTgyOSANCkl0IHdvcmtzIGdyZWF0IG9uIHJhdCB0aXNzdWUhDQoNCjIwMDktMDQtMjQgDQoNCg0K DQpURiANCg0KDQoNCreivP7Iy6O6IFNtaXRoIFdhbmRhIA0Kt6LLzcqxvOSjuiAyMDA5LTA0LTIz ICAwMzoxNjozNiANCsrVvP7Iy6O6IGhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdSAN CrOty82juiANCtb3zOKjuiBbSGlzdG9uZXRdIE15ZWxvcGVyb3hpZGFzZSBBbnRpYm9keSANCiAN Ckdvb2QgQWZ0ZXJub29uIEV2ZXJ5b25lLA0KV2hlcmUgZG8geW91IHJlY29tbWVuZCBvcmRlcmlu ZyB0aGUgcHJlLWRpbHV0ZSBhbnRpYm9keSBNeWVsb3Blcm94aWRhc2UgTVBPIEFiLTI/ICBCaW9j YXJlIGFuZCBMYWIgVmlzaW9uIGhhdmUgYm90aCBkaXNjb250aW51ZWQgY2FycnlpbmcgaXQuICBQ bGVhc2UgaGVscCEhISEhDQpUaGFua3MsDQpXYW5kYQ0KV0FOREEgRy4gU01JVEgsIEhUTChBU0NQ KUhUDQpQYXRob2xvZ3kgU3VwZXJ2aXNvcg0KVFJJREVOVCBNRURJQ0FMIENFTlRFUg0KOTMzMCBN ZWRpY2FsIFBsYXphIERyaXZlDQpDaGFybGVzdG9uLCBTQyAgMjk0MDYNCjg0My04NDctNDU4Ng0K ODQzLTg0Ny00Mjk2IGZheA0KX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19f X19fX19fX18NCkhpc3RvbmV0IG1haWxpbmcgbGlzdA0KSGlzdG9uZXRAbGlzdHMudXRzb3V0aHdl c3Rlcm4uZWR1DQpodHRwOi8vbGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1L21haWxtYW4vbGlzdGlu Zm8vaGlzdG9uZXQNCg== From CIngles <@t> uwhealth.org Thu Apr 23 13:52:08 2009 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Thu Apr 23 13:52:13 2009 Subject: [Histonet] commercial control slides References: <1101A09566B25D4BB3889A44FDC1E7040A9B18@uantexchg03.capitalhealth.ca> Message-ID: Steve: I would think of keeping a frozen block of fatty tissue around and cutting fresh control when you do the stain or throw some precut slides in the freezer after fixation, as the specimen needs to be fresh frozen anyway and cannot come in contact with any alcohols. Skin is an OK tissue, as the sebaceous glands as well as the subdermal fat lights up. I would think it would be pretty difficult, if not impossible to ship slides that need to be kept frozen to prevent staining sensitivity degradation. I can only think of shipping them immersed in formalin? Claire Can anyone recommend a brand or line of commercial control slides for oil red O? Thanks, Steve Joy, BSc. MLT Research and Development Technologist 5B2.03 Anatomical Pathology University of Alberta Hospital 8440-112 st Edmonton Ab T6G 2B7 Phone: (780) 407-8015 Fax: (780) 407-3009 Email: steven.joy@capitalhealth.ca This communication is intended for the sole use of the recipient to which it was addressed and may contain confidential, personal or privileged information. Please contact the sender immediately if you are not the intended recipient of this information and do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Apr 23 15:03:16 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Apr 23 15:03:23 2009 Subject: AW: [Histonet] ground meat samples In-Reply-To: References: Message-ID: <2696E9BF0D3D4D3681AAC57D3B6150E1@dielangs.at> I think producing a "cytoblock" with histogel or similar could help. You can also take citratplasma and coagulate it to a small block with the meat-pieces inside. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Perry, Margaret Gesendet: Donnerstag, 23. April 2009 18:53 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] ground meat samples We will be doing IHC on ground pork and would like tips on how to process and embed the samples. Can you leave the processed sample in a paper biopsy bag and embed both the meat and the paper? Will cutting the paper make the blade dull to quickly? Is there anything else we can use to hold the tissue together? We had thought to maybe use pig intestine and pack the meat into it to make it cut better. Thank you in advance for your help. Histonet has helped me out many times and I greatly appreciate it. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Thu Apr 23 18:47:20 2009 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Thu Apr 23 18:48:37 2009 Subject: [Histonet] ground meat samples Message-ID: <688810.59118.qm@web45101.mail.sp1.yahoo.com> I?thing handling it like a curretting?wrapped in lens paper or the sausage?ideas will both work well. I will add make sure your tissue is no more than 3 mm thick?when you process it. Probably better to be a bit thinner. I imagine there will be some beef fat in there and assume it will?need ?good penetration. Stephen Peters M.D.? Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 Phone and fax 201 847 7600 www.pathologyinnovations.com From jnocito <@t> satx.rr.com Thu Apr 23 19:39:12 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Apr 23 19:39:26 2009 Subject: [Histonet] commercial control slides In-Reply-To: <1101A09566B25D4BB3889A44FDC1E7040A9B18@uantexchg03.capitalhealth.ca> References: <1101A09566B25D4BB3889A44FDC1E7040A9B18@uantexchg03.capitalhealth.ca> Message-ID: <3569DFB947964FD2B2054ABF331AB343@JoePC> this is hard to do because of the frozen section requirement. However, we make smears out of mayonnaise when we have Oil Red O stain and it works well. Good luck JTT ----- Original Message ----- From: "Steven Joy" To: Sent: Thursday, April 23, 2009 1:38 PM Subject: [Histonet] commercial control slides Can anyone recommend a brand or line of commercial control slides for oil red O? Thanks, Steve Joy, BSc. MLT Research and Development Technologist 5B2.03 Anatomical Pathology University of Alberta Hospital 8440-112 st Edmonton Ab T6G 2B7 Phone: (780) 407-8015 Fax: (780) 407-3009 Email: steven.joy@capitalhealth.ca This communication is intended for the sole use of the recipient to which it was addressed and may contain confidential, personal or privileged information. Please contact the sender immediately if you are not the intended recipient of this information and do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Apr 23 19:47:17 2009 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Apr 23 19:47:31 2009 Subject: [Histonet] Grossing In-Reply-To: <435583.27358.qm@web58908.mail.re1.yahoo.com> References: <435583.27358.qm@web58908.mail.re1.yahoo.com> Message-ID: <628FD19EBC8F444593AA7DAE461F282B@JoePC> Schaundra, we have residents and 2 PAs perform all the grossing. In real tight situations, we have a couple of qualified grossing histotechs we can rely on in a pinch. we have histotechs set up the specimens, but the residents and PA's gross alone. We are responsible for annotating any special procedures that need to be ordered. Our grossing cutoff time is 5:30 PM because of the start time of the tissue processors (we start embedding at 3:00 AM). JTT ----- Original Message ----- From: "Schaundra Walton" To: "Histonet" Sent: Thursday, April 23, 2009 1:25 PM Subject: [Histonet] Grossing We are evaluating some of our grossing procedures and cut off times and I was wondering what other people are doing. Who does your grossing, pathologist, PA, or other qualified individuals? Who does you accessioning, histotechs, support staff, or other? What is your cut off time for grossing tissues? Right now our pathologists gross all tissues, the histotechs accession, and grossing is done up until 6pm (which is when the processor begins). Thanks for the info! -Schaundra Walton HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Thu Apr 23 20:14:54 2009 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Apr 23 20:15:06 2009 Subject: [Histonet] commercial control slides In-Reply-To: <1101A09566B25D4BB3889A44FDC1E7040A9B18@uantexchg03.capitalhealth.ca> Message-ID: <779326963F2F4FF0964AFCF96B0452A4@HPPav2> Frozen section of adrenal (from autopsy) is a great control. Freeze, cut 50-100 sections, put in a slide box, put in freezer, good for about a year. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Joy Sent: Thursday, April 23, 2009 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] commercial control slides Can anyone recommend a brand or line of commercial control slides for oil red O? Thanks, Steve Joy, BSc. MLT Research and Development Technologist 5B2.03 Anatomical Pathology University of Alberta Hospital 8440-112 st Edmonton Ab T6G 2B7 Phone: (780) 407-8015 Fax: (780) 407-3009 Email: steven.joy@capitalhealth.ca This communication is intended for the sole use of the recipient to which it was addressed and may contain confidential, personal or privileged information. Please contact the sender immediately if you are not the intended recipient of this information and do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sarah_tarran <@t> wmi.usyd.edu.au Thu Apr 23 23:58:08 2009 From: sarah_tarran <@t> wmi.usyd.edu.au (Sarah Tarran) Date: Fri Apr 24 01:14:56 2009 Subject: [Histonet] non-specific fluorescence in artery Message-ID: <2183.172.19.58.90.1240549088.squirrel@www.wmi.usyd.edu.au> Hi Histonetters, We have a problem. We have optimised LSP-1 (leukocyte specific protein) on spleen that has been fixed in 4% PFA/PBS for 2h, incubated in 18%sucrose/PBS overnight and then embedded in OCT. We now want to test it on our arteries that have been fixed and frozen the same way as the spleen. The staining protocol used is also identical for both tissues (see below). However, unlike the specific staining we observe in spleen (on the leukocytes), we observe very obvious non-specific background in the arteries. Pretty much all of the stroma surrounding the tissue is positively staining. The negative controls look good. Could anyone please offer any reasons for what we're seeing? Our method is: H2O to get rid of OCT Citrate buffer - 10min at 60C (H2O bath) Wash in running water - 10 min Soaked 0.3%Triton/PBS - 3changes, 3 min each Dako Protein block - 10 min Primary Ab (rabbit anti-mouse), 1:50 diln - 1h TBS with tween - 3x 3 changes secondary Ab (Alexa Fluor 594, goat anti-rabbit), 1:800 - 1h TBS with tween - 3x 3 changes DAPI Thanks, Sarah Sarah Tarran Postdoctoral Fellow Vascular Biology Research Centre, Department of Surgery Westmead Hospital, Westmead, NSW, 2145 sarah_tarran@wmi.usyd.edu.au From tifei <@t> foxmail.com Fri Apr 24 05:25:20 2009 From: tifei <@t> foxmail.com (TF) Date: Fri Apr 24 05:25:40 2009 Subject: [Histonet] Doublecortin staining with Abcam antibody References: <5998F3BDFF7AAC4091C7AE93A7A1A5892CDF8F@UWHC-MAIL01.uwhis.hosp.wisc.edu> Message-ID: <200904241825152967272@foxmail.com> hi all, I am staining 40 um brain frozen sections with Abcam anti-DCX (rabbit source). With 10% goat serum blocking for 1 hour before primary antibody, I observed a lot of non-specific fluorescence on my slide. Anyone experienced this before? Second, I did not perform the antigen retrieval before the staining. The sections are made on a microtome, and floating sections are then mounted on slides. But I added 0.3% triton to the primary antibody dilution. Will very long incubation (1-2 nights room temperature) with high concentration of triton affect the staining result? Such as "leaking" pattern of staining. 2009-04-24 TF From alyssa <@t> alliedsearchpartners.com Fri Apr 24 09:23:14 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Fri Apr 24 09:23:18 2009 Subject: [Histonet] Opening In Texas Message-ID: Happy Friday Everyone, I have an opening for a histotech in Corpus Christi, TX. The position is full time/permanent, and full relocation is paid. Please let me know if you or anyone you know would be interested in this position. Thank you! ** *Position: Histology Technician* Department:Histology Specialty Area:Allied Health Supervisor's Title:Histology Manager Work Schedule:FT Early Morning* MONDAY-FRIDAY WITH WEEKEND ROTATION* ------------------------------ Experience Required: ? Approximately 6moths-1 year on-the-job experience necessary Job Description: ? Performs various histological procedures to prepare and process tissue specimens for examination by pathologist. ? Provides information for diagnosis and treatment by conducting histological procedures following policies and procedures. Education: ? HT certification by Board of Registry of the American Society of Clinical Pathologists. *Benefits: * ? 1. Highly competitive compensation package, compensation continuously reviewed and updated. Relocation Assistance. Medical Benefits, and much more! -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 From ian.montgomery <@t> bio.gla.ac.uk Fri Apr 24 09:45:38 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Apr 24 09:49:33 2009 Subject: [Histonet] Mega cassettes Message-ID: <706A809EABE9491C8BB67361AF32FBBC@IBLS.GLA.AC.UK> Looking for a supplier of mega cassettes in the UK. VWR are a wee bit expensive. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From Montina.VanMeter <@t> pbrc.edu Fri Apr 24 10:33:50 2009 From: Montina.VanMeter <@t> pbrc.edu (Montina Van Meter) Date: Fri Apr 24 10:34:00 2009 Subject: [Histonet] Doublecortin staining with Abcam antibody References: <5998F3BDFF7AAC4091C7AE93A7A1A5892CDF8F@UWHC-MAIL01.uwhis.hosp.wisc.edu> <200904241825152967272@foxmail.com> Message-ID: <4FE7FB862E90E448AE32388E759220E5DC1757@pbrcas31.pbrc.edu> Tifei, I routinely section rat brain at 40 microns and free-float them through the immuno protocol. Here are a few suggestions: 1. Did you perform a dilution series to optimize the best antibody concentration? 2. I antigen retrieve difficult antibodies (especially with thick sections) in a Decloaker (Biocare), thus opening up the binding sites. 3. I'm assuming the tissue is fixed - we incubate the sections in a 1% sodium borohydride solution (after Decloaker step) to rid excess aldehyde in tissue prior to the blocking step (20-30 mins.) Follow with PBS or TBS rinses. 3. We also use 0.3% triton - this is not a high concentration for 40 um sections. 4. I use 0.3% triton in my antibody diluent - overnight incubation. 5. Make sure you have at least 3 rinses in between incubations to rid nonspecific "glow garbage" from the secondary antibody. Hope this helps, Tina Montina J. Van Meter Lab Manager Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70808 225-763-2564 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of TF Sent: Fri 4/24/2009 5:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Doublecortin staining with Abcam antibody hi all, I am staining 40 um brain frozen sections with Abcam anti-DCX (rabbit source). With 10% goat serum blocking for 1 hour before primary antibody, I observed a lot of non-specific fluorescence on my slide. Anyone experienced this before? Second, I did not perform the antigen retrieval before the staining. The sections are made on a microtome, and floating sections are then mounted on slides. But I added 0.3% triton to the primary antibody dilution. Will very long incubation (1-2 nights room temperature) with high concentration of triton affect the staining result? Such as "leaking" pattern of staining. 2009-04-24 TF _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera <@t> msu.edu Fri Apr 24 10:39:39 2009 From: portera <@t> msu.edu (Amy Porter) Date: Fri Apr 24 10:39:41 2009 Subject: [Histonet] Mega cassettes References: <706A809EABE9491C8BB67361AF32FBBC@IBLS.GLA.AC.UK> Message-ID: <907F27D1D1AA41C9AF86E0AD4373C4F6@histolab> You could try Surgipath Medical. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Ian Montgomery" To: Sent: Friday, April 24, 2009 10:45 AM Subject: [Histonet] Mega cassettes > Looking for a supplier of mega cassettes in the UK. VWR are a > wee bit expensive. > > Ian. > > > > > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mcadams <@t> u.washington.edu Thu Apr 23 19:43:09 2009 From: mcadams <@t> u.washington.edu (Ryan McAdams) Date: Fri Apr 24 10:58:58 2009 Subject: [Histonet] paraffin embedding Message-ID: I am trying to embed adult rat aortas in paraffin for histology purposes. Do you have any recommendations on an optimal method to keep the aortas in a vertical position while the paraffin solidifies? I am hoping to do transverse sectioning of the thoracic aortas to analyze wall thickness. Thanks, Ryan Ryan McAdams Assistant Professor of Pediatrics Division of Neonatology University of Washington Box 356320 Seattle, WA 98195-6320 Telephone: (206)-616-8246 From AMaruska <@t> mngastro.com Fri Apr 24 11:53:23 2009 From: AMaruska <@t> mngastro.com (Maruska, Ann) Date: Fri Apr 24 11:53:27 2009 Subject: [Histonet] benzene/paraffin Message-ID: Hi Histonetters, In reviewing the MSDS sheets in our histology lab, I noticed that the GemCut paraffin we are using contains 2% benzene. I am concerned about this. Is this the exception or the norm? Does other paraffin contain products that would be considered hazardous or are they labeled in such a manner that the consumer is unaware? If someone is using a product that they know is safe and performs with high quality, please let me know. Thanks! Ann Ann Maruska Pathology Lab manager Minnesota Gastroenterology email: amaruska@mngastro.com office: 651-605-3070 cell: 612-462-9699 fax: 612-870-5863 From histonet.nospam <@t> vneubert.com Fri Apr 24 13:07:12 2009 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Fri Apr 24 13:07:17 2009 Subject: [Histonet] paraffin embedding In-Reply-To: References: Message-ID: <49F1FFD0.6050104@vneubert.com> Take some paraffin into the mould, then grab your tissue, press it to the bottom, hold it and get onto cooling plate. Wait a few seconds for the paraffin to start getting solid, then fill up with hot paraffin. I use this "technique" everyday. Ryan McAdams wrote: > I am trying to embed adult rat aortas in paraffin for histology purposes. > Do you have any recommendations on an optimal method to keep the aortas in a > vertical position while the paraffin solidifies? I am hoping to do > transverse sectioning of the thoracic aortas to analyze wall thickness. > > > > Thanks, > > > > Ryan > > > > Ryan McAdams > > Assistant Professor of Pediatrics > > Division of Neonatology University of Washington > > Box 356320 Seattle, WA 98195-6320 > > Telephone: (206)-616-8246 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cbarone <@t> NEMOURS.ORG Fri Apr 24 14:06:41 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Fri Apr 24 14:06:43 2009 Subject: [Histonet] RE: Is this ok for Histonet? In-Reply-To: Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A71EA@wlmmsx01.nemours.org> Is this OK for the Histonet: ATTENTION!:VA, PA, MD, NJ, DE, W. VA, and DC Earn CEU's at a state hosted regional meeting to be held in Virginia Beach, VA. Saturday, May 16th. Hosted by NJ,DE,MD and PA...One day event/ earn up to 5 CEU's. Hosted by PA, MD, DE, and NJ. Sponsored by Biocare. For more info contact C. Barone @ 302.651.6827. Will send Registration Information! DEADLINE for pre-reg in May 12th. -Antibody Panels in the Anatomic Laboratory -Multiplex Stains for the Anatomic Pathology Laboratory -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, April 24, 2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 65, Issue 42 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: ground meat samples (Charles, Roger) 2. RE: ground meat samples (Rittman, Barry R) 3. Ground meat processing (Breeden, Sara) 4. RE: levamisole (Shakun Aswani) 5. Re: EM Knife (Geoff McAuliffe) 6. new vs used microtomes(regular rotary) (Madary, Joseph) 7. Grossing (Schaundra Walton) 8. Fatty Breast Tissue processing (Bull, Jennifer L.) 9. Herpes (Mark Tarango) 10. ground meat processing (Dolores_Fischer@baxter.com) 11. commercial control slides (Steven Joy) 12. Re: Myeloperoxidase Antibody (TF) 13. RE: commercial control slides (Ingles Claire ) 14. AW: [Histonet] ground meat samples (Gudrun Lang) 15. ground meat samples (Stephen Peters M.D.) 16. Re: commercial control slides (Joe Nocito) 17. Re: Grossing (Joe Nocito) 18. RE: commercial control slides (Lee & Peggy Wenk) 19. non-specific fluorescence in artery (Sarah Tarran) 20. Doublecortin staining with Abcam antibody (TF) 21. Opening In Texas (Alyssa Peterson) 22. Mega cassettes (Ian Montgomery) 23. RE: Doublecortin staining with Abcam antibody (Montina Van Meter) 24. Re: Mega cassettes (Amy Porter) 25. paraffin embedding (Ryan McAdams) 26. benzene/paraffin (Maruska, Ann) ---------------------------------------------------------------------- Message: 1 Date: Thu, 23 Apr 2009 13:20:37 -0400 From: "Charles, Roger" Subject: [Histonet] RE: ground meat samples To: "histonet@lists.utsouthwestern.edu" Message-ID: <3809C163DC1DA54AA534B3C7794D07B632A1C80A45@ENHBGMBX01.PA.LCL> Content-Type: text/plain; charset="us-ascii" Sorry Histonetter's But it is my Friday and wouldn't this method give new meaning to the term "sausage block." :) I wish Margaret all the luck in her quest and if it was me, I would try either or both methods. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rcharles@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Thursday, April 23, 2009 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ground meat samples We will be doing IHC on ground pork and would like tips on how to process and embed the samples. Can you leave the processed sample in a paper biopsy bag and embed both the meat and the paper? Will cutting the paper make the blade dull to quickly? Is there anything else we can use to hold the tissue together? We had thought to maybe use pig intestine and pack the meat into it to make it cut better. Thank you in advance for your help. Histonet has helped me out many times and I greatly appreciate it. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 23 Apr 2009 12:48:17 -0500 From: "Rittman, Barry R" Subject: [Histonet] RE: ground meat samples To: "histonet@lists.utsouthwestern.edu" Message-ID: <75A0543E23D3A7458012D9E02EDBEC0002E9248A46@UTHCMS1.uthouston.edu> Content-Type: text/plain; charset="us-ascii" Roger If I were you I would compress this into a block before fixation. Can use paper up to wax but would not use paper covering in the embedded block it will make cutting too difficult. Alternative is to use sausage skin as the covering and process, embed and cut with this. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Thursday, April 23, 2009 12:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: ground meat samples Sorry Histonetter's But it is my Friday and wouldn't this method give new meaning to the term "sausage block." :) I wish Margaret all the luck in her quest and if it was me, I would try either or both methods. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 rcharles@state.pa.us No trees were hurt in the sending of this email, However many electrons were severely inconvenienced! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Thursday, April 23, 2009 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ground meat samples We will be doing IHC on ground pork and would like tips on how to process and embed the samples. Can you leave the processed sample in a paper biopsy bag and embed both the meat and the paper? Will cutting the paper make the blade dull to quickly? Is there anything else we can use to hold the tissue together? We had thought to maybe use pig intestine and pack the meat into it to make it cut better. Thank you in advance for your help. Histonet has helped me out many times and I greatly appreciate it. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Thu, 23 Apr 2009 11:50:06 -0600 From: "Breeden, Sara" Subject: [Histonet] Ground meat processing To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E682B@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" What would be wrong with just submitting the ground sample as one would with a curetting or similar - that is if the pieces are small, submit them for processing in a lens paper packet or one of those paper histo bags, then scrape the pieces off the paper and embed into paraffin as usual? Perhaps I'm thrown off a bit by the part about embedding the paper along with the tissue? Do you have some special requirement for processing for IHC that makes it necessary to consider embedding the paper? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 4 Date: Thu, 23 Apr 2009 10:52:15 -0700 From: "Shakun Aswani" Subject: RE: [Histonet] levamisole To: "anjan kumar" , "triple immunohistochem" Message-ID: <777AB0DE519C8E46A6220E2287C5BAD301B34E9E@EXCHANGE.acologix.com> Content-Type: text/plain; charset="US-ASCII" Hi Anjan, Here is the molecular weight of the levamisole, from this you can calculate the 125mM Levamisole Analytical Information * Synonym: leva (l)-Tetramisole * Chemical Name: (6S)-2,3,5,6-Tetrahydro-6-phenylimidazo[2,1-b]thiazole * Chemical Formula: C11H12N2S*HCl * Molecular Weight: 204.3 * Formula Weight: 240.8 * pKa: 8.0 * Levamisole is extracted using a basic liquid-liquid chlorobutane extraction with an acid back extraction. * Levamisole is easily detected on the GC/NPD and GC/MS. * Elution Order: Doxylamine, LEVAMISOLE, Chlorpheniramine I hope this will help you Regards, Shakun Note: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anjan kumar Sent: Wednesday, April 22, 2009 1:42 PM To: triple immunohistochem Subject: [Histonet] levamisole hi everyone, can anyone tell me how to prepare 125mM of levamisole. Dr. Anjan Kumar.K.R M.V.Sc Scholar Dept. of Veterinary Pathology Madras Veterinary College Chennai-7 India email: drvet_anjan@hotmail.com Phone: +91-9940475801 _________________________________________________________________ How fun is this? IMing with Windows Live Messenger just got better. http://www.microsoft.com/india/windows/windowslive/messenger.aspx_______ ________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 23 Apr 2009 13:56:57 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] EM Knife To: Stella Mireles Cc: Histonet@lists.utsouthwestern.edu Message-ID: <49F0ABE9.5050704@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi Stella: All of our knives are Diatome and I have no complaints. On the other hand, I have been doing business with Pelco for many years with no problems ever. It seems unlilely that they are making their own knives so they are probably buying and rebranding. You will probably be fine with either. Geoff Stella Mireles wrote: > All EM Techs. Which Ultra knife is better. Diatome or Pelco? Is there any > other company that I should consider. I plan to purchase an Ultra and a > Histo knife. > Thank You > Stella > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 6 Date: Thu, 23 Apr 2009 14:08:05 -0400 From: "Madary, Joseph" Subject: [Histonet] new vs used microtomes(regular rotary) To: Message-ID: Content-Type: text/plain; charset="us-ascii" I bought a couple of Microms and so far they have been great. Wondering if anyone has purchased a new rotary microtome recently for a decent price that they like. I have bought a couple of used as well many years ago from a guy I trusted no longer in the business. Any suggestions there? I have purchased used equipment from IMEB and BelAir both have been fine. Just checking my options here. I have an RMS that I never really bothered using that has electronic advance/retract that works fine but I am a little old school and that little feature is more annoying where some folks love it. If there are any vendors out there who deal in used equipment and would give me something on a trade in, that might make me consider you over someone else. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ------------------------------ Message: 7 Date: Thu, 23 Apr 2009 11:25:22 -0700 (PDT) From: Schaundra Walton Subject: [Histonet] Grossing To: Histonet Message-ID: <435583.27358.qm@web58908.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We are evaluating some of our grossing procedures and cut off times and I was wondering what other people are doing. ? Who does your grossing, pathologist, PA, or other qualified individuals? Who does you accessioning, histotechs, support staff, or other? What is your cut off time for grossing tissues? ? Right now our pathologists gross all tissues, the histotechs accession, and grossing is done up until 6pm (which is when the processor begins). ? Thanks for the info! ? -Schaundra Walton HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL ------------------------------ Message: 8 Date: Thu, 23 Apr 2009 11:27:07 -0700 From: "Bull, Jennifer L." Subject: [Histonet] Fatty Breast Tissue processing To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <85760CECEC18444BB95F26D5E88DAEAA223F261C25@hinet2.hinet.org> Content-Type: text/plain; charset="us-ascii" Is anyone willing to share their protocol for processing fatty breast tissue? It is difficult to find any documentation or processes that work really well. Thank you in advance, you have all been extremely helpful to me! Jennifer Bull Histology Supervisor jennifer.bull@nwpathology.com ------------------------------ Message: 9 Date: Thu, 23 Apr 2009 11:36:23 -0700 From: Mark Tarango Subject: [Histonet] Herpes To: "histonet@lists.utsouthwestern.edu" Message-ID: <5b6eb13e0904231136j4bccbb31i55c757c86e053378@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Does anyone have a herpes control for which they'd be willing to trade? I can trade just about any tumor or control material you might need.... thanks Mark Tarango ------------------------------ Message: 10 Date: Thu, 23 Apr 2009 13:37:01 -0500 From: Dolores_Fischer@baxter.com Subject: [Histonet] ground meat processing To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" I agree with Sally, why not just process as a curretting by loosely placing pieces of ground meat in a cassette and embed in an appropriate sized mold for your IHC needs. Paper will shred, it does not section well, if at all. Dolores Fischer The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer ------------------------------ Message: 11 Date: Thu, 23 Apr 2009 12:38:58 -0600 From: "Steven Joy" Subject: [Histonet] commercial control slides To: Message-ID: <1101A09566B25D4BB3889A44FDC1E7040A9B18@uantexchg03.capitalhealth.ca> Content-Type: text/plain; charset="US-ASCII" Can anyone recommend a brand or line of commercial control slides for oil red O? Thanks, Steve Joy, BSc. MLT Research and Development Technologist 5B2.03 Anatomical Pathology University of Alberta Hospital 8440-112 st Edmonton Ab T6G 2B7 Phone: (780) 407-8015 Fax: (780) 407-3009 Email: steven.joy@capitalhealth.ca This communication is intended for the sole use of the recipient to which it was addressed and may contain confidential, personal or privileged information. Please contact the sender immediately if you are not the intended recipient of this information and do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. Thank you. ------------------------------ Message: 12 Date: Fri, 24 Apr 2009 02:44:12 +0800 From: "TF" Subject: Re: [Histonet] Myeloperoxidase Antibody To: "Smith Wanda" , "histonet@lists.utsouthwestern.edu" Message-ID: <200904240244042496604@foxmail.com> Content-Type: text/plain; charset="gb2312" Hi, we use Rb a Hu Myeloperoxidase Dakocytomat A039829 It works great on rat tissue! 2009-04-24 TF ???????? Smith Wanda ?????????? 2009-04-23 03:16:36 ???????? histonet@lists.utsouthwestern.edu ?????? ?????? [Histonet] Myeloperoxidase Antibody Good Afternoon Everyone, Where do you recommend ordering the pre-dilute antibody Myeloperoxidase MPO Ab-2? Biocare and Lab Vision have both discontinued carrying it. Please help!!!!! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Thu, 23 Apr 2009 13:52:08 -0500 From: "Ingles Claire " Subject: RE: [Histonet] commercial control slides To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Steve: I would think of keeping a frozen block of fatty tissue around and cutting fresh control when you do the stain or throw some precut slides in the freezer after fixation, as the specimen needs to be fresh frozen anyway and cannot come in contact with any alcohols. Skin is an OK tissue, as the sebaceous glands as well as the subdermal fat lights up. I would think it would be pretty difficult, if not impossible to ship slides that need to be kept frozen to prevent staining sensitivity degradation. I can only think of shipping them immersed in formalin? Claire Can anyone recommend a brand or line of commercial control slides for oil red O? Thanks, Steve Joy, BSc. MLT Research and Development Technologist 5B2.03 Anatomical Pathology University of Alberta Hospital 8440-112 st Edmonton Ab T6G 2B7 Phone: (780) 407-8015 Fax: (780) 407-3009 Email: steven.joy@capitalhealth.ca This communication is intended for the sole use of the recipient to which it was addressed and may contain confidential, personal or privileged information. Please contact the sender immediately if you are not the intended recipient of this information and do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 23 Apr 2009 22:03:16 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] ground meat samples To: "'Perry, Margaret'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <2696E9BF0D3D4D3681AAC57D3B6150E1@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" I think producing a "cytoblock" with histogel or similar could help. You can also take citratplasma and coagulate it to a small block with the meat-pieces inside. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Perry, Margaret Gesendet: Donnerstag, 23. April 2009 18:53 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] ground meat samples We will be doing IHC on ground pork and would like tips on how to process and embed the samples. Can you leave the processed sample in a paper biopsy bag and embed both the meat and the paper? Will cutting the paper make the blade dull to quickly? Is there anything else we can use to hold the tissue together? We had thought to maybe use pig intestine and pack the meat into it to make it cut better. Thank you in advance for your help. Histonet has helped me out many times and I greatly appreciate it. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 23 Apr 2009 16:47:20 -0700 (PDT) From: "Stephen Peters M.D." Subject: [Histonet] ground meat samples To: histonet@lists.utsouthwestern.edu Message-ID: <688810.59118.qm@web45101.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I?thing handling it like a curretting?wrapped in lens paper or the sausage?ideas will both work well. I will add make sure your tissue is no more than 3 mm thick?when you process it. Probably better to be a bit thinner. I imagine there will be some beef fat in there and assume it will?need ?good penetration. Stephen Peters M.D.? Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 Phone and fax 201 847 7600 www.pathologyinnovations.com ------------------------------ Message: 16 Date: Thu, 23 Apr 2009 19:39:12 -0500 From: "Joe Nocito" Subject: Re: [Histonet] commercial control slides To: "Steven Joy" , Message-ID: <3569DFB947964FD2B2054ABF331AB343@JoePC> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original this is hard to do because of the frozen section requirement. However, we make smears out of mayonnaise when we have Oil Red O stain and it works well. Good luck JTT ----- Original Message ----- From: "Steven Joy" To: Sent: Thursday, April 23, 2009 1:38 PM Subject: [Histonet] commercial control slides Can anyone recommend a brand or line of commercial control slides for oil red O? Thanks, Steve Joy, BSc. MLT Research and Development Technologist 5B2.03 Anatomical Pathology University of Alberta Hospital 8440-112 st Edmonton Ab T6G 2B7 Phone: (780) 407-8015 Fax: (780) 407-3009 Email: steven.joy@capitalhealth.ca This communication is intended for the sole use of the recipient to which it was addressed and may contain confidential, personal or privileged information. Please contact the sender immediately if you are not the intended recipient of this information and do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 23 Apr 2009 19:47:17 -0500 From: "Joe Nocito" Subject: Re: [Histonet] Grossing To: , "Histonet" Message-ID: <628FD19EBC8F444593AA7DAE461F282B@JoePC> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Schaundra, we have residents and 2 PAs perform all the grossing. In real tight situations, we have a couple of qualified grossing histotechs we can rely on in a pinch. we have histotechs set up the specimens, but the residents and PA's gross alone. We are responsible for annotating any special procedures that need to be ordered. Our grossing cutoff time is 5:30 PM because of the start time of the tissue processors (we start embedding at 3:00 AM). JTT ----- Original Message ----- From: "Schaundra Walton" To: "Histonet" Sent: Thursday, April 23, 2009 1:25 PM Subject: [Histonet] Grossing We are evaluating some of our grossing procedures and cut off times and I was wondering what other people are doing. Who does your grossing, pathologist, PA, or other qualified individuals? Who does you accessioning, histotechs, support staff, or other? What is your cut off time for grossing tissues? Right now our pathologists gross all tissues, the histotechs accession, and grossing is done up until 6pm (which is when the processor begins). Thanks for the info! -Schaundra Walton HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Thu, 23 Apr 2009 21:14:54 -0400 From: "Lee & Peggy Wenk" Subject: RE: [Histonet] commercial control slides To: "'Steven Joy'" , Message-ID: <779326963F2F4FF0964AFCF96B0452A4@HPPav2> Content-Type: text/plain; charset="us-ascii" Frozen section of adrenal (from autopsy) is a great control. Freeze, cut 50-100 sections, put in a slide box, put in freezer, good for about a year. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Joy Sent: Thursday, April 23, 2009 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] commercial control slides Can anyone recommend a brand or line of commercial control slides for oil red O? Thanks, Steve Joy, BSc. MLT Research and Development Technologist 5B2.03 Anatomical Pathology University of Alberta Hospital 8440-112 st Edmonton Ab T6G 2B7 Phone: (780) 407-8015 Fax: (780) 407-3009 Email: steven.joy@capitalhealth.ca This communication is intended for the sole use of the recipient to which it was addressed and may contain confidential, personal or privileged information. Please contact the sender immediately if you are not the intended recipient of this information and do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Fri, 24 Apr 2009 14:58:08 +1000 (EST) From: "Sarah Tarran" Subject: [Histonet] non-specific fluorescence in artery To: histonet@lists.utsouthwestern.edu Message-ID: <2183.172.19.58.90.1240549088.squirrel@www.wmi.usyd.edu.au> Content-Type: text/plain;charset=iso-8859-1 Hi Histonetters, We have a problem. We have optimised LSP-1 (leukocyte specific protein) on spleen that has been fixed in 4% PFA/PBS for 2h, incubated in 18%sucrose/PBS overnight and then embedded in OCT. We now want to test it on our arteries that have been fixed and frozen the same way as the spleen. The staining protocol used is also identical for both tissues (see below). However, unlike the specific staining we observe in spleen (on the leukocytes), we observe very obvious non-specific background in the arteries. Pretty much all of the stroma surrounding the tissue is positively staining. The negative controls look good. Could anyone please offer any reasons for what we're seeing? Our method is: H2O to get rid of OCT Citrate buffer - 10min at 60C (H2O bath) Wash in running water - 10 min Soaked 0.3%Triton/PBS - 3changes, 3 min each Dako Protein block - 10 min Primary Ab (rabbit anti-mouse), 1:50 diln - 1h TBS with tween - 3x 3 changes secondary Ab (Alexa Fluor 594, goat anti-rabbit), 1:800 - 1h TBS with tween - 3x 3 changes DAPI Thanks, Sarah Sarah Tarran Postdoctoral Fellow Vascular Biology Research Centre, Department of Surgery Westmead Hospital, Westmead, NSW, 2145 sarah_tarran@wmi.usyd.edu.au ------------------------------ Message: 20 Date: Fri, 24 Apr 2009 18:25:20 +0800 From: "TF" Subject: [Histonet] Doublecortin staining with Abcam antibody To: "histonet@lists.utsouthwestern.edu" Message-ID: <200904241825152967272@foxmail.com> Content-Type: text/plain; charset="us-ascii" hi all, I am staining 40 um brain frozen sections with Abcam anti-DCX (rabbit source). With 10% goat serum blocking for 1 hour before primary antibody, I observed a lot of non-specific fluorescence on my slide. Anyone experienced this before? Second, I did not perform the antigen retrieval before the staining. The sections are made on a microtome, and floating sections are then mounted on slides. But I added 0.3% triton to the primary antibody dilution. Will very long incubation (1-2 nights room temperature) with high concentration of triton affect the staining result? Such as "leaking" pattern of staining. 2009-04-24 TF ------------------------------ Message: 21 Date: Fri, 24 Apr 2009 10:23:14 -0400 From: Alyssa Peterson Subject: [Histonet] Opening In Texas To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Happy Friday Everyone, I have an opening for a histotech in Corpus Christi, TX. The position is full time/permanent, and full relocation is paid. Please let me know if you or anyone you know would be interested in this position. Thank you! ** *Position: Histology Technician* Department:Histology Specialty Area:Allied Health Supervisor's Title:Histology Manager Work Schedule:FT Early Morning* MONDAY-FRIDAY WITH WEEKEND ROTATION* ------------------------------ Experience Required: ? Approximately 6moths-1 year on-the-job experience necessary Job Description: ? Performs various histological procedures to prepare and process tissue specimens for examination by pathologist. ? Provides information for diagnosis and treatment by conducting histological procedures following policies and procedures. Education: ? HT certification by Board of Registry of the American Society of Clinical Pathologists. *Benefits: * ? 1. Highly competitive compensation package, compensation continuously reviewed and updated. Relocation Assistance. Medical Benefits, and much more! -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 ------------------------------ Message: 22 Date: Fri, 24 Apr 2009 15:45:38 +0100 From: "Ian Montgomery" Subject: [Histonet] Mega cassettes To: Message-ID: <706A809EABE9491C8BB67361AF32FBBC@IBLS.GLA.AC.UK> Content-Type: text/plain; charset="us-ascii" Looking for a supplier of mega cassettes in the UK. VWR are a wee bit expensive. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. ------------------------------ Message: 23 Date: Fri, 24 Apr 2009 10:33:50 -0500 From: "Montina Van Meter" Subject: RE: [Histonet] Doublecortin staining with Abcam antibody To: , Message-ID: <4FE7FB862E90E448AE32388E759220E5DC1757@pbrcas31.pbrc.edu> Content-Type: text/plain; charset="iso-8859-1" Tifei, I routinely section rat brain at 40 microns and free-float them through the immuno protocol. Here are a few suggestions: 1. Did you perform a dilution series to optimize the best antibody concentration? 2. I antigen retrieve difficult antibodies (especially with thick sections) in a Decloaker (Biocare), thus opening up the binding sites. 3. I'm assuming the tissue is fixed - we incubate the sections in a 1% sodium borohydride solution (after Decloaker step) to rid excess aldehyde in tissue prior to the blocking step (20-30 mins.) Follow with PBS or TBS rinses. 3. We also use 0.3% triton - this is not a high concentration for 40 um sections. 4. I use 0.3% triton in my antibody diluent - overnight incubation. 5. Make sure you have at least 3 rinses in between incubations to rid nonspecific "glow garbage" from the secondary antibody. Hope this helps, Tina Montina J. Van Meter Lab Manager Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70808 225-763-2564 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of TF Sent: Fri 4/24/2009 5:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Doublecortin staining with Abcam antibody hi all, I am staining 40 um brain frozen sections with Abcam anti-DCX (rabbit source). With 10% goat serum blocking for 1 hour before primary antibody, I observed a lot of non-specific fluorescence on my slide. Anyone experienced this before? Second, I did not perform the antigen retrieval before the staining. The sections are made on a microtome, and floating sections are then mounted on slides. But I added 0.3% triton to the primary antibody dilution. Will very long incubation (1-2 nights room temperature) with high concentration of triton affect the staining result? Such as "leaking" pattern of staining. 2009-04-24 TF _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 24 Date: Fri, 24 Apr 2009 11:39:39 -0400 From: "Amy Porter" Subject: Re: [Histonet] Mega cassettes To: , Message-ID: <907F27D1D1AA41C9AF86E0AD4373C4F6@histolab> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original You could try Surgipath Medical. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Ian Montgomery" To: Sent: Friday, April 24, 2009 10:45 AM Subject: [Histonet] Mega cassettes > Looking for a supplier of mega cassettes in the UK. VWR are a > wee bit expensive. > > Ian. > > > > > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 25 Date: Thu, 23 Apr 2009 17:43:09 -0700 From: "Ryan McAdams" Subject: [Histonet] paraffin embedding To: Message-ID: Content-Type: text/plain; charset="us-ascii" I am trying to embed adult rat aortas in paraffin for histology purposes. Do you have any recommendations on an optimal method to keep the aortas in a vertical position while the paraffin solidifies? I am hoping to do transverse sectioning of the thoracic aortas to analyze wall thickness. Thanks, Ryan Ryan McAdams Assistant Professor of Pediatrics Division of Neonatology University of Washington Box 356320 Seattle, WA 98195-6320 Telephone: (206)-616-8246 ------------------------------ Message: 26 Date: Fri, 24 Apr 2009 11:53:23 -0500 From: "Maruska, Ann" Subject: [Histonet] benzene/paraffin To: "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Histonetters, In reviewing the MSDS sheets in our histology lab, I noticed that the GemCut paraffin we are using contains 2% benzene. I am concerned about this. Is this the exception or the norm? Does other paraffin contain products that would be considered hazardous or are they labeled in such a manner that the consumer is unaware? If someone is using a product that they know is safe and performs with high quality, please let me know. Thanks! Ann Ann Maruska Pathology Lab manager Minnesota Gastroenterology email: amaruska@mngastro.com office: 651-605-3070 cell: 612-462-9699 fax: 612-870-5863 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 65, Issue 42 **************************************** From gagnone <@t> KGH.KARI.NET Fri Apr 24 14:52:41 2009 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Fri Apr 24 14:52:48 2009 Subject: [Histonet] Control slides for Oil Red O Message-ID: I was always of the opinion that since a delipidized frozen section, treated with toluene/methanol, run in parallel with the patient slide constituted adequate control. Recently though, I've mellowed and realized that only shows a difference in staining between the two slides, not the effectiveness of your stain. Using a piece of fat for a control slide confirms microscopically what we can confirm macroscopically...yep, it's a piece of fat. The adrenal and skin ideas sound better to me than using a piece of breast/fatty tissue. The era of performing staining methods without controls seems to be at an end, which isn't necessarily a bad thing. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario,Canada From estellamireles <@t> gmail.com Fri Apr 24 15:16:27 2009 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Fri Apr 24 15:16:35 2009 Subject: [Histonet] EM Techs -RMC powertome ultramicrotome question Message-ID: Is anyone using this ultramicrotome? I would like know how it compares to a Leica ultramicrotome? Thanks Stella From sprice2003 <@t> gmail.com Fri Apr 24 16:58:40 2009 From: sprice2003 <@t> gmail.com (Sally Price) Date: Fri Apr 24 16:58:45 2009 Subject: [Histonet] Need refurbished H&E stainer Message-ID: Fellow 'Netters: I'm helping a friend set up a new lab and they'd like to get a refurbished H&E stainer. Any suggestions, including likely price range, will be greatly appreciated. The lab's located in Ohio, if that makes a difference to potential sellers. Thx, Sally From Timothy.Morken <@t> ucsfmedctr.org Fri Apr 24 17:44:00 2009 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Tim) Date: Fri Apr 24 17:44:07 2009 Subject: [Histonet] New postiion Message-ID: <1AAF670737F193429070841C6B2ADD4C01A9093A@EXMBMCB15.ucsfmedicalcenter.org> For all my friends and acquaintances on Histonet, please note I have left Thermo Scientific and am now at University of California San Francisco Medical Center. My contact information: Tim Morken, HTL (ASCP) Supervisor, Histology / IPOX UCSF Medical Center Box 1656 1600 Divisadero St. San Francisco, CA 94143-1656 USA Phone: (415) 514-6042 Pager: (415) 443-6509 Fax: (415) 885-7409 Email: tim.morken@ucsfmedctr.org From amosbrooks <@t> gmail.com Fri Apr 24 23:57:06 2009 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Apr 24 23:57:11 2009 Subject: [Histonet] ground meat samples Message-ID: <582736990904242157n3150a0f0qbbff2c1743142ee9@mail.gmail.com> Hi Margaret, I would do whatever I could to avoid cutting paper. Sounds like a nightmare that could be avoided. The suggestion of Histogel has merit, but the fact that it is often difficult to cut is a limiting factor as well as the cost to use it compared to a much cheaper alternative. This would probably require some experimentation to find out what would work best, but you had mentioned using pig intestine as a casing. Pig intestine might be considered a contaminent as you probably don't want the same intestinal surface in all the samples. There are alternatives of course. Other non intestinal casings can be used. There are collagenous casings as well as synthetic casings that would be a much better alternative and at $10- $12 it is a more cost effective option. You may even find these at your local butcher. (Check Kosher products) As to which type will cut best, that is where the experimentation will come in. A quick check came up with quite a few alternatives, check: http://www.sausagemaker.com/index.asp?PageAction=VIEWCATS&Category=118 This sounds like a facinating project, Amos Message: 23 Date: Thu, 23 Apr 2009 11:53:28 -0500 From: "Perry, Margaret" Subject: [Histonet] ground meat samples To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We will be doing IHC on ground pork and would like tips on how to process and embed the samples. Can you leave the processed sample in a paper biopsy bag and embed both the meat and the paper? Will cutting the paper make the blade dull to quickly? Is there anything else we can use to hold the tissue together? We had thought to maybe use pig intestine and pack the meat into it to make it cut better. Thank you in advance for your help. Histonet has helped me out many times and I greatly appreciate it. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From rjbuesa <@t> yahoo.com Sat Apr 25 08:55:40 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Apr 25 08:55:49 2009 Subject: [Histonet] Productivity in histology Message-ID: <997415.5727.qm@web65711.mail.ac4.yahoo.com> Dear Colleagues: I am trying to expand?the data base on histology productivity from the study I did in 2005 and, as always, will need your help. ? All of you who are interested in contributing and receive the summary that this study will produce, please get in contact with me so I can send you the "Productivity form". ? I am interested in histology labs working in human and veterinary pathology?from the US and also from any other country. ? I hope to receive your cooperation?for this study ? Ren? J. From nicole.rahde <@t> terra.com.br Sat Apr 25 10:40:32 2009 From: nicole.rahde <@t> terra.com.br (nicole.rahde@terra.com.br) Date: Sat Apr 25 10:40:37 2009 Subject: [Histonet] TRAP staining for osteoclasts Message-ID: <1916.1240674032@terra.com.br> Hi! I am having trouble with TRAP staining on formalin fixed, paraffin embedded, formic acid decalcified rat bone. The positive control (wich was not decalcified) showed beautifully marked osteoclasts, but the rat bone did not at all. Would the formic acid used in decalcification impair the reaction? Does anyone know something I could do to to make the reaction work on these specimens? Thanks a lot! Best regards, Nicole Rahde DDS, MSc, PhD student, Oral Medicine, PUC-RS Porto Alegre, Brazil. From mshaeffer <@t> cox.net Sat Apr 25 12:15:50 2009 From: mshaeffer <@t> cox.net (Marc Shaeffer) Date: Sat Apr 25 12:16:00 2009 Subject: [Histonet] paraffin embedding Message-ID: Don't forget to put the plastic cassette on top the mold prior to filling and solidifying the paraffin. From mikael.niku <@t> helsinki.fi Sat Apr 25 15:24:56 2009 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Sat Apr 25 15:25:09 2009 Subject: [Histonet] Long term storage for IHC? Message-ID: <49F37198.5030907@helsinki.fi> Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland From tifei <@t> foxmail.com Sun Apr 26 00:19:53 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Sun Apr 26 00:20:37 2009 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gTG9uZyB0ZXJtIHN0b3JhZ2UgZm9yIElIQz8=?= References: <49F37198.5030907@helsinki.fi> Message-ID: <200904261319483656380@foxmail.com> Hi, i embeded all of them in OCT and put them under -20. To prevent water vapor leakage, use some parafilm to pack the OCT block or seal it. We tested this in samples harvested 5 years ago (2004-2009). 2009-04-26 TF ???? Mikael Niku ????? 2009-04-26 04:31:05 ???? histonet ??? ??? [Histonet] Long term storage for IHC? Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sun Apr 26 05:46:50 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Apr 26 05:47:00 2009 Subject: AW: [Histonet] Long term storage for IHC? In-Reply-To: <49F37198.5030907@helsinki.fi> References: <49F37198.5030907@helsinki.fi> Message-ID: <7224DDE4D9664677A8281EECFDDD9309@dielangs.at> I guess the formalin-fixation will be reversed although in a very slow manner in the 70% ethanol. And I think, overnight fixation depending on the tissue size will give insufficient formalin-fixation. This will result in ethanol-fixation in the 70% and especially in the 100% ethanol. If you want long time storage in ethanol, be sure that formalin-fixation is sufficient. In my opinion your regular immunhistoprotocol has also to be adapted after long time storage (more than 2 months), no matter if in formaldehyd or ethanol. What is more effort? Storage and administration of large numbers of wet tissue in refrigerator or processing to paraffin blocks and storage in drawers? Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Mikael Niku Gesendet: Samstag, 25. April 2009 22:25 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Long term storage for IHC? Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Apr 26 09:38:53 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Apr 26 09:38:58 2009 Subject: [Histonet] Long term storage for IHC ? Message-ID: <863080.7096.qm@web65704.mail.ac4.yahoo.com> Hi Mikael: Long term storage of?formalin fixed?tissue is usually never a good option. If you keep them in NBF the antigenic sites could be so compromised that their unmasking could prove to be unsuccessful. In 70% EthOL they will macerate and, as you point out, could end as if they were alcohol fixed. There are really 2?options: 1- process the tissues and? save the uncut blocks, or 2- select the most interesting pieces?and fix them for 48 hours to assure full fixation and after washing in PBS freeze them and keep them?frozen at -80?C. When the moment arises that you will need them, thaw and process them. I think that? you should go with cryoperservation. I am attaching an article I wrote about formalin fixation. Ren? J. ? Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From janet.dertien <@t> ttuhsc.edu Sun Apr 26 14:16:58 2009 From: janet.dertien <@t> ttuhsc.edu (Dertien, Janet) Date: Sun Apr 26 14:19:05 2009 Subject: [Histonet] help References: <20090426170521.1C41F164FADA@lub12bar100702.ttuhsc.edu> Message-ID: Hello, I have a friend who is having trouble with ziehl-neesen stainin. Her sections appear red (with no blue). I have a feeling she may not be destaining adequately. Any suggestions? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of histonet-request@lists.utsouthwestern.edu Sent: Sun 4/26/2009 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 65, Issue 44 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. paraffin embedding (Marc Shaeffer) 2. Long term storage for IHC? (Mikael Niku) 3. Re: Long term storage for IHC? ( TF ) 4. AW: [Histonet] Long term storage for IHC? (Gudrun Lang) 5. Long term storage for IHC ? (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Sat, 25 Apr 2009 10:15:50 -0700 From: "Marc Shaeffer" Subject: [Histonet] paraffin embedding To: Message-ID: Content-Type: text/plain; charset="us-ascii" Don't forget to put the plastic cassette on top the mold prior to filling and solidifying the paraffin. ------------------------------ Message: 2 Date: Sat, 25 Apr 2009 23:24:56 +0300 From: Mikael Niku Subject: [Histonet] Long term storage for IHC? To: histonet@lists.utsouthwestern.edu Message-ID: <49F37198.5030907@helsinki.fi> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland ------------------------------ Message: 3 Date: Sun, 26 Apr 2009 13:19:53 +0800 From: " TF " Subject: Re: [Histonet] Long term storage for IHC? To: " Mikael Niku " , " histonet " Message-ID: <200904261319483656380@foxmail.com> Content-Type: text/plain; charset="utf-8" Hi, i embeded all of them in OCT and put them under -20. To prevent water vapor leakage, use some parafilm to pack the OCT block or seal it. We tested this in samples harvested 5 years ago (2004-2009). 2009-04-26 TF ??'????????s Mikael Niku ??'????-??-???s 2009-04-26 04:31:05 ?"?????????s histonet ?S"?????s ?????~??s [Histonet] Long term storage for IHC? Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sun, 26 Apr 2009 12:46:50 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Long term storage for IHC? To: "'Mikael Niku'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <7224DDE4D9664677A8281EECFDDD9309@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" I guess the formalin-fixation will be reversed although in a very slow manner in the 70% ethanol. And I think, overnight fixation depending on the tissue size will give insufficient formalin-fixation. This will result in ethanol-fixation in the 70% and especially in the 100% ethanol. If you want long time storage in ethanol, be sure that formalin-fixation is sufficient. In my opinion your regular immunhistoprotocol has also to be adapted after long time storage (more than 2 months), no matter if in formaldehyd or ethanol. What is more effort? Storage and administration of large numbers of wet tissue in refrigerator or processing to paraffin blocks and storage in drawers? Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Mikael Niku Gesendet: Samstag, 25. April 2009 22:25 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Long term storage for IHC? Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Sun, 26 Apr 2009 07:38:53 -0700 (PDT) From: Rene J Buesa Subject: [Histonet] Long term storage for IHC ? To: histonet@lists.utsouthwestern.edu Message-ID: <863080.7096.qm@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Mikael: Long term storage of?formalin fixed?tissue is usually never a good option. If you keep them in NBF the antigenic sites could be so compromised that their unmasking could prove to be unsuccessful. In 70% EthOL they will macerate and, as you point out, could end as if they were alcohol fixed. There are really 2?options: 1- process the tissues and? save the uncut blocks, or 2- select the most interesting pieces?and fix them for 48 hours to assure full fixation and after washing in PBS freeze them and keep them?frozen at -80?C. When the moment arises that you will need them, thaw and process them. I think that? you should go with cryoperservation. I am attaching an article I wrote about formalin fixation. Ren? J. ? Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 65, Issue 44 **************************************** From rjbuesa <@t> yahoo.com Sun Apr 26 14:33:39 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Apr 26 14:33:44 2009 Subject: [Histonet] help In-Reply-To: Message-ID: <787723.60379.qm@web65711.mail.ac4.yahoo.com> Your advise to her is correct: probably the sections are not destained completely. Any section stained with the Ziehl-Nielsen Carbol Fucsin has to be treated with acid alcohol until no more red color leaches from the section no matter how much time that takes. No more red coming out of the section is the end point of the destaining. Ren? J.? --- On Sun, 4/26/09, Dertien, Janet wrote: From: Dertien, Janet Subject: [Histonet] help To: histonet@lists.utsouthwestern.edu Date: Sunday, April 26, 2009, 3:16 PM Hello, I have a friend who is having trouble with ziehl-neesen stainin. Her sections appear red (with no blue). I have a feeling she may not be destaining adequately. Any suggestions? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of histonet-request@lists.utsouthwestern.edu Sent: Sun 4/26/2009 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 65, Issue 44 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. paraffin embedding (Marc Shaeffer) 2. Long term storage for IHC? (Mikael Niku) 3. Re: Long term storage for IHC? ( TF ) 4. AW: [Histonet] Long term storage for IHC? (Gudrun Lang) 5. Long term storage for IHC ? (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Sat, 25 Apr 2009 10:15:50 -0700 From: "Marc Shaeffer" Subject: [Histonet] paraffin embedding To: Message-ID: Content-Type: text/plain; charset="us-ascii" Don't forget to put the plastic cassette on top the mold prior to filling and solidifying the paraffin. ------------------------------ Message: 2 Date: Sat, 25 Apr 2009 23:24:56 +0300 From: Mikael Niku Subject: [Histonet] Long term storage for IHC? To: histonet@lists.utsouthwestern.edu Message-ID: <49F37198.5030907@helsinki.fi> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland ------------------------------ Message: 3 Date: Sun, 26 Apr 2009 13:19:53 +0800 From: " TF " Subject: Re: [Histonet] Long term storage for IHC? To: " Mikael Niku " , " histonet " Message-ID: <200904261319483656380@foxmail.com> Content-Type: text/plain; charset="utf-8" Hi, i embeded all of them in OCT and put them under -20. To prevent water vapor leakage, use some parafilm to pack the OCT block or seal it. We tested this in samples harvested 5 years ago (2004-2009). 2009-04-26 TF ??'????????s Mikael Niku ??'????-??-???s 2009-04-26 04:31:05 ?"?????????s histonet ?S"?????s ?????~??s [Histonet] Long term storage for IHC? Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sun, 26 Apr 2009 12:46:50 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Long term storage for IHC? To: "'Mikael Niku'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <7224DDE4D9664677A8281EECFDDD9309@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" I guess the formalin-fixation will be reversed although in a very slow manner in the 70% ethanol. And I think, overnight fixation depending on the tissue size will give insufficient formalin-fixation. This will result in ethanol-fixation in the 70% and especially in the 100% ethanol. If you want long time storage in ethanol, be sure that formalin-fixation is sufficient. In my opinion your regular immunhistoprotocol has also to be adapted after long time storage (more than 2 months), no matter if in formaldehyd or ethanol. What is more effort? Storage and administration of large numbers of wet tissue in refrigerator or processing to paraffin blocks and storage in drawers? Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Mikael Niku Gesendet: Samstag, 25. April 2009 22:25 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Long term storage for IHC? Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Sun, 26 Apr 2009 07:38:53 -0700 (PDT) From: Rene J Buesa Subject: [Histonet] Long term storage for IHC ? To: histonet@lists.utsouthwestern.edu Message-ID: <863080.7096.qm@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Mikael: Long term storage of?formalin fixed?tissue is usually never a good option. If you keep them in NBF the antigenic sites could be so compromised that their unmasking could prove to be unsuccessful. In 70% EthOL they will macerate and, as you point out, could end as if they were alcohol fixed. There are really 2?options: 1- process the tissues and? save the uncut blocks, or 2- select the most interesting pieces?and fix them for 48 hours to assure full fixation and after washing in PBS freeze them and keep them?frozen at -80?C. When the moment arises that you will need them, thaw and process them. I think that? you should go with cryoperservation. I am attaching an article I wrote about formalin fixation. Ren? J. ? Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 65, Issue 44 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfarish <@t> csu.edu.au Sun Apr 26 18:31:07 2009 From: cfarish <@t> csu.edu.au (Farish, Craig) Date: Sun Apr 26 18:31:19 2009 Subject: [Histonet] RE Finding Iron in Decalcified Sections Message-ID: <79A000B60AFE8045BA2581119DFEC44405625926@ESWW01.CSUMain.csu.edu.au> Thanks to all who have offered me advice and ideas. I've taken it on board and I'll try a few new approaches in the coming weeks. I'll let you know the outcome. Cheers, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga, Australia c farish@csu.edu.au ' I don't want to achieve immortality through my work, I want to achieve immortality through not dying' - Woody Allen From tifei <@t> foxmail.com Sun Apr 26 23:13:18 2009 From: tifei <@t> foxmail.com (TF) Date: Sun Apr 26 23:13:47 2009 Subject: [Histonet] Doublecortin, Ki67, Caspase-3: need antigen retrieval ? Message-ID: <200904271213137323714@foxmail.com> Hi all i am working on brain. After perfusion with 4% PFA, I put the brain in the fixative for post-fixation overnight. Then I put the brain into 30% surcose until sink. Then I perform cryostat or microtome sections. Do you think it is necessary to perform antigen retrieval of DCX (doublecortin), ki67, and caspase-3? I got very weak staining signals. I am not sure whether it is due to the low primary incubation concentration? Or insufficient antigen binding site exposure? 2009-04-27 TF From cfarish <@t> csu.edu.au Mon Apr 27 01:23:01 2009 From: cfarish <@t> csu.edu.au (Farish, Craig) Date: Mon Apr 27 01:23:59 2009 Subject: [Histonet] RE Megacassettes Message-ID: <79A000B60AFE8045BA2581119DFEC44405625B5A@ESWW01.CSUMain.csu.edu.au> Hi Ian, Try Cellpath - www.cellpath.co.uk. It was cheaper for me to buy them from the UK and pay for shipping than it was to buy them in Australia. According to the catalogue they have a distributor in Cumbernauld called Surgical Supply Services but they're general number is 01686 611333. Good luck, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Boorooma Street Wagga Wagga NSW 2678 Australia "I don't want to achieve immortality through my work, I want to achieve immortality through not dying" Woody Allen From Stella_quan <@t> DNAR.com Mon Apr 27 06:59:20 2009 From: Stella_quan <@t> DNAR.com (Stella_quan@DNAR.com) Date: Mon Apr 27 06:59:40 2009 Subject: [Histonet] RE: Histonet Digest, Vol 65, Issue 44 Message-ID: <380-220094127115920885@M2W035.mail2web.com> Hi Bill, You can look under California Society of Histology Meeting held in Millbrae CA in May. If you need any other information, please let me know. Have a great day! Best Stella Original Message: ----------------- From: histonet-request@lists.utsouthwestern.edu histonet-request@lists.utsouthwestern.edu Date: Sun, 26 Apr 2009 13:08:30 -0400 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 65, Issue 44 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. paraffin embedding (Marc Shaeffer) 2. Long term storage for IHC? (Mikael Niku) 3. Re: Long term storage for IHC? ( TF ) 4. AW: [Histonet] Long term storage for IHC? (Gudrun Lang) 5. Long term storage for IHC ? (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Sat, 25 Apr 2009 10:15:50 -0700 From: "Marc Shaeffer" Subject: [Histonet] paraffin embedding To: Message-ID: Content-Type: text/plain; charset="us-ascii" Don't forget to put the plastic cassette on top the mold prior to filling and solidifying the paraffin. ------------------------------ Message: 2 Date: Sat, 25 Apr 2009 23:24:56 +0300 From: Mikael Niku Subject: [Histonet] Long term storage for IHC? To: histonet@lists.utsouthwestern.edu Message-ID: <49F37198.5030907@helsinki.fi> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland ------------------------------ Message: 3 Date: Sun, 26 Apr 2009 13:19:53 +0800 From: " TF " Subject: Re: [Histonet] Long term storage for IHC? To: " Mikael Niku " , " histonet " Message-ID: <200904261319483656380@foxmail.com> Content-Type: text/plain; charset="utf-8" Hi, i embeded all of them in OCT and put them under -20. To prevent water vapor leakage, use some parafilm to pack the OCT block or seal it. We tested this in samples harvested 5 years ago (2004-2009). 2009-04-26 TF ???????????? Mikael Niku ??????????????? 2009-04-26 04:31:05 ???????????? histonet ????????? ????????? [Histonet] Long term storage for IHC? Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sun, 26 Apr 2009 12:46:50 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Long term storage for IHC? To: "'Mikael Niku'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <7224DDE4D9664677A8281EECFDDD9309@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" I guess the formalin-fixation will be reversed although in a very slow manner in the 70% ethanol. And I think, overnight fixation depending on the tissue size will give insufficient formalin-fixation. This will result in ethanol-fixation in the 70% and especially in the 100% ethanol. If you want long time storage in ethanol, be sure that formalin-fixation is sufficient. In my opinion your regular immunhistoprotocol has also to be adapted after long time storage (more than 2 months), no matter if in formaldehyd or ethanol. What is more effort? Storage and administration of large numbers of wet tissue in refrigerator or processing to paraffin blocks and storage in drawers? Gudrun Lang -----Urspr???ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Mikael Niku Gesendet: Samstag, 25. April 2009 22:25 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Long term storage for IHC? Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Sun, 26 Apr 2009 07:38:53 -0700 (PDT) From: Rene J Buesa Subject: [Histonet] Long term storage for IHC ? To: histonet@lists.utsouthwestern.edu Message-ID: <863080.7096.qm@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Mikael: Long term storage of???formalin fixed???tissue is usually never a good option. If you keep them in NBF the antigenic sites could be so compromised that their unmasking could prove to be unsuccessful. In 70% EthOL they will macerate and, as you point out, could end as if they were alcohol fixed. There are really 2???options: 1- process the tissues and??? save the uncut blocks, or 2- select the most interesting pieces???and fix them for 48 hours to assure full fixation and after washing in PBS freeze them and keep them???frozen at -80???C. When the moment arises that you will need them, thaw and process them. I think that??? you should go with cryoperservation. I am attaching an article I wrote about formalin fixation. Ren??? J. ??? Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 65, Issue 44 **************************************** -------------------------------------------------------------------- mail2web.com ? What can On Demand Business Solutions do for you? http://link.mail2web.com/Business/SharePoint From dchihc <@t> yahoo.com Mon Apr 27 09:02:16 2009 From: dchihc <@t> yahoo.com (Phyllis Thaxton) Date: Mon Apr 27 09:02:23 2009 Subject: [Histonet] AMMONIUM HYDROXIDE Message-ID: <183835.78611.qm@web43509.mail.sp1.yahoo.com> Hi all, ? I looked in archives to see if I could find any info on this but did not. Remember, we are having a problem with some of the sections floating off during IHC.? ?? If?paraffin blocks are faced and then placed? in ammonium hydroxide before cutting the H&E,?would this cause the sections to float off if the unstained sections that are cut in addition to the H&E's are also produced in this manner? Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From LSebree <@t> uwhealth.org Mon Apr 27 09:06:07 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Apr 27 09:06:10 2009 Subject: [Histonet] AMMONIUM HYDROXIDE In-Reply-To: <183835.78611.qm@web43509.mail.sp1.yahoo.com> Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A5892CDF98@UWHC-MAIL01.uwhis.hosp.wisc.edu> We've never seen that correlation Phyllis but that doesn't mean there isn't one. Our ammonium hydroxide is used at 5% followed by chilling the block, then sectioning. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phyllis Thaxton Sent: Monday, April 27, 2009 9:02 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] AMMONIUM HYDROXIDE Hi all, ? I looked in archives to see if I could find any info on this but did not. Remember, we are having a problem with some of the sections floating off during IHC.? ?? If?paraffin blocks are faced and then placed? in ammonium hydroxide before cutting the H&E,?would this cause the sections to float off if the unstained sections that are cut in addition to the H&E's are also produced in this manner? Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Mon Apr 27 09:07:10 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Apr 27 09:07:21 2009 Subject: [Histonet] AMMONIUM HYDROXIDE In-Reply-To: <183835.78611.qm@web43509.mail.sp1.yahoo.com> References: <183835.78611.qm@web43509.mail.sp1.yahoo.com> Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE101AD2F83@LTA3VS011.ees.hhs.gov> Phyllis, I have used ammonia frequently in the past for soaking certain troublesome blocks but it has never resulted in sections washing off. We do use Plus slides, though. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phyllis Thaxton Sent: Monday, April 27, 2009 10:02 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] AMMONIUM HYDROXIDE Hi all, ? I looked in archives to see if I could find any info on this but did not. Remember, we are having a problem with some of the sections floating off during IHC. ?? If?paraffin blocks are faced and then placed? in ammonium hydroxide before cutting the H&E,?would this cause the sections to float off if the unstained sections that are cut in addition to the H&E's are also produced in this manner? Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Mon Apr 27 09:17:27 2009 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Apr 27 09:17:40 2009 Subject: AW: [Histonet] help In-Reply-To: References: <20090426170521.1C41F164FADA@lub12bar100702.ttuhsc.edu> Message-ID: Methylenblue doesn't adhere to the tissue properly, if the acid isn't washed out well with tapwater. The staining pH has to be alkalineto make a overall stain. Methylenblue is washed out easily in diluted ethanols. Just wash in tapwater, blot on paper and let dry for a few seconds and dehydrate quickly in absolute ethanol to xylen (or similar). Some tissue (especially connective tissue) tends to refuse methylenblue staining. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Dertien, Janet Gesendet: Sonntag, 26. April 2009 21:17 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] help Hello, I have a friend who is having trouble with ziehl-neesen stainin. Her sections appear red (with no blue). I have a feeling she may not be destaining adequately. Any suggestions? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of histonet-request@lists.utsouthwestern.edu Sent: Sun 4/26/2009 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 65, Issue 44 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. paraffin embedding (Marc Shaeffer) 2. Long term storage for IHC? (Mikael Niku) 3. Re: Long term storage for IHC? ( TF ) 4. AW: [Histonet] Long term storage for IHC? (Gudrun Lang) 5. Long term storage for IHC ? (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Sat, 25 Apr 2009 10:15:50 -0700 From: "Marc Shaeffer" Subject: [Histonet] paraffin embedding To: Message-ID: Content-Type: text/plain; charset="us-ascii" Don't forget to put the plastic cassette on top the mold prior to filling and solidifying the paraffin. ------------------------------ Message: 2 Date: Sat, 25 Apr 2009 23:24:56 +0300 From: Mikael Niku Subject: [Histonet] Long term storage for IHC? To: histonet@lists.utsouthwestern.edu Message-ID: <49F37198.5030907@helsinki.fi> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland ------------------------------ Message: 3 Date: Sun, 26 Apr 2009 13:19:53 +0800 From: " TF " Subject: Re: [Histonet] Long term storage for IHC? To: " Mikael Niku " , " histonet " Message-ID: <200904261319483656380@foxmail.com> Content-Type: text/plain; charset="utf-8" Hi, i embeded all of them in OCT and put them under -20. To prevent water vapor leakage, use some parafilm to pack the OCT block or seal it. We tested this in samples harvested 5 years ago (2004-2009). 2009-04-26 TF ??'????????s Mikael Niku ??'????-??-???s 2009-04-26 04:31:05 ?"?????????s histonet ?S"?????s ?????~??s [Histonet] Long term storage for IHC? Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sun, 26 Apr 2009 12:46:50 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] Long term storage for IHC? To: "'Mikael Niku'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <7224DDE4D9664677A8281EECFDDD9309@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" I guess the formalin-fixation will be reversed although in a very slow manner in the 70% ethanol. And I think, overnight fixation depending on the tissue size will give insufficient formalin-fixation. This will result in ethanol-fixation in the 70% and especially in the 100% ethanol. If you want long time storage in ethanol, be sure that formalin-fixation is sufficient. In my opinion your regular immunhistoprotocol has also to be adapted after long time storage (more than 2 months), no matter if in formaldehyd or ethanol. What is more effort? Storage and administration of large numbers of wet tissue in refrigerator or processing to paraffin blocks and storage in drawers? Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Mikael Niku Gesendet: Samstag, 25. April 2009 22:25 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Long term storage for IHC? Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Sun, 26 Apr 2009 07:38:53 -0700 (PDT) From: Rene J Buesa Subject: [Histonet] Long term storage for IHC ? To: histonet@lists.utsouthwestern.edu Message-ID: <863080.7096.qm@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Mikael: Long term storage of?formalin fixed?tissue is usually never a good option. If you keep them in NBF the antigenic sites could be so compromised that their unmasking could prove to be unsuccessful. In 70% EthOL they will macerate and, as you point out, could end as if they were alcohol fixed. There are really 2?options: 1- process the tissues and? save the uncut blocks, or 2- select the most interesting pieces?and fix them for 48 hours to assure full fixation and after washing in PBS freeze them and keep them?frozen at -80?C. When the moment arises that you will need them, thaw and process them. I think that? you should go with cryoperservation. I am attaching an article I wrote about formalin fixation. Ren? J. ? Dear Histonetters, what do you think is the best way to store formalin-fixed tissues for immunohistochemistry, long-term (several years)? We are currently fixing overnight, then rinsing in buffer and storing in 70% or 100% ethanol, +4C or -20C. However, I don't really KNOW what happens here and how the tissues will work after the years. I guess basically the formalin fixation will be at least somewhat reversed and after some time the tissues will be more like ethanol fixed. But are there better options? Long term storage in formalin will make IHC difficult. And this is about large numbers of tissue samples, only some of which will be actually used later, so we wouldn't like to process all of them to paraffin either. With best regards, Mikael Niku University of Helsinki, Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 65, Issue 44 **************************************** From DixonM <@t> vetmed.ufl.edu Mon Apr 27 09:24:39 2009 From: DixonM <@t> vetmed.ufl.edu (MaryAnn Dixon) Date: Mon Apr 27 09:24:43 2009 Subject: [Histonet] HT exam Message-ID: <530D827EC657DE418C3572ADD63FCDC3249724@EXGVMCNETWORK.vetmed.ufl.edu> Hey histonetters, Just wanted to tell you all that I PASSED my HT exam this past Saturday. Thank you to all for your wonderful advice and support! MaryAnn Dixon BS, HT (ASCP) Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 352-2235 Ext. 4517 From mucram11 <@t> comcast.net Mon Apr 27 09:45:09 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Mon Apr 27 09:45:12 2009 Subject: [Histonet] HT exam In-Reply-To: <530D827EC657DE418C3572ADD63FCDC3249724@EXGVMCNETWORK.vetmed.ufl.edu> Message-ID: <1081425617.39121240843509881.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> CONGRADULATIONS!!! Pam Marcum UPENN Vet Sch New Bolton Center ----- Original Message ----- From: "MaryAnn Dixon" To: histonet@lists.utsouthwestern.edu Sent: Monday, April 27, 2009 10:24:39 AM GMT -05:00 US/Canada Eastern Subject: [Histonet] HT exam Hey histonetters, ? Just wanted to tell you all that I PASSED my HT exam this ?past Saturday. ?Thank you to all for your wonderful advice and support! ? ? MaryAnn Dixon BS, HT (ASCP) Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 352-2235 Ext. 4517 ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jessgrocki <@t> yahoo.com Mon Apr 27 09:59:38 2009 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Mon Apr 27 09:59:50 2009 Subject: [Histonet] Sensitivity and Specificity of Antibodies for IHC Message-ID: <248047.21047.qm@web82001.mail.mud.yahoo.com> Thanks to everyone who responded to my previous email regarding negative control tissue for antibodies. We are looking for tissue that will not stain with the antibodies I listed. ? I guess what I should have asked is what kind of procedure are all of you doing to stain for sensitivity and specificity of your antibodies? If anyone would like to share their procedure, that would be great! ? Hope everyone had a great weekend and thanks again! ? Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital CT From stephanie.d.rivera <@t> gsk.com Mon Apr 27 10:05:36 2009 From: stephanie.d.rivera <@t> gsk.com (stephanie.d.rivera@gsk.com) Date: Mon Apr 27 10:06:05 2009 Subject: [Histonet] HT exam In-Reply-To: <1081425617.39121240843509881.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: Congratulations MaryAnn! Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 "Pamela Marcum" Sent by: histonet-bounces@lists.utsouthwestern.edu 27-Apr-2009 10:45 To "MaryAnn Dixon" cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] HT exam CONGRADULATIONS!!! Pam Marcum UPENN Vet Sch New Bolton Center ----- Original Message ----- From: "MaryAnn Dixon" To: histonet@lists.utsouthwestern.edu Sent: Monday, April 27, 2009 10:24:39 AM GMT -05:00 US/Canada Eastern Subject: [Histonet] HT exam Hey histonetters, Just wanted to tell you all that I PASSED my HT exam this past Saturday. Thank you to all for your wonderful advice and support! MaryAnn Dixon BS, HT (ASCP) Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 352-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sharon.Davis-Devine <@t> carle.com Mon Apr 27 10:07:42 2009 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Mon Apr 27 10:07:49 2009 Subject: [Histonet] Embedding issues Message-ID: <44780C571F28624DBB446DE55C4D733A04257CFD@EXCHANGEBE1.carle.com> We are having issues with our embedding. We are routinely seeing bubbles when we go to cut the blocks. Our embedders are very careful about tamping the tissue down to prevent this but it still seems to be happening. We have narrowed down to one particular cassette so that may be the problem. We were just wondering is anyone out there in Histoland has had similar problems and if you have how did you solve it? As always thanks for the help. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com From Kathleen.Caleri <@t> RoswellPark.org Mon Apr 27 10:39:40 2009 From: Kathleen.Caleri <@t> RoswellPark.org (Caleri, Kathleen) Date: Mon Apr 27 10:38:48 2009 Subject: [Histonet] breast tissue Message-ID: "Could everyone please share their "method of transportation" for breast tissue-from surgery to the gross room? Is it being placed immediately into formalin? or brought up fresh? If you need fresh tissue for procurement, how is that handled?" This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From ree3 <@t> leicester.ac.uk Mon Apr 27 10:40:59 2009 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Apr 27 10:41:30 2009 Subject: [Histonet] RE: haemotol autostainer? In-Reply-To: <7722595275A4DD4FA225B92CDBF174A17457783D1A@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A17457783D1A@EXC-MBX3.cfs.le.ac.uk> Message-ID: <7722595275A4DD4FA225B92CDBF174A17457783D35@EXC-MBX3.cfs.le.ac.uk> A colleague is looking to purchase a staining machine, primarily for Romanowsky type staining, any recommendations or brick bats please. Richard Edwards Leicester University...UK. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Apr 27 11:03:33 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 27 11:03:42 2009 Subject: [Histonet] AMMONIUM HYDROXIDE In-Reply-To: <183835.78611.qm@web43509.mail.sp1.yahoo.com> Message-ID: <8649.17270.qm@web65716.mail.ac4.yahoo.com> If it was strong ("pure" or 28%) ammonium hydroxide, and the sections were not completely drained after placing them in the slides, that could be the cause. Ren? J. --- On Mon, 4/27/09, Phyllis Thaxton wrote: From: Phyllis Thaxton Subject: [Histonet] AMMONIUM HYDROXIDE To: Histonet@lists.utsouthwestern.edu Date: Monday, April 27, 2009, 10:02 AM Hi all, ? I looked in archives to see if I could find any info on this but did not. Remember, we are having a problem with some of the sections floating off during IHC.? ?? If?paraffin blocks are faced and then placed? in ammonium hydroxide before cutting the H&E,?would this cause the sections to float off if the unstained sections that are cut in addition to the H&E's are also produced in this manner? Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From putnamj <@t> ggclinic.com Mon Apr 27 11:03:11 2009 From: putnamj <@t> ggclinic.com (Putnam, Jodi) Date: Mon Apr 27 11:03:52 2009 Subject: [Histonet] Fontana Masson Message-ID: Hello. Do any of you know of a source for a kit for the Fontana Masson? I am used to doing it on the bench and making up all the solutions, but under the circumstances, a kit might be better. I have checked Sigma and Newcomersupply so far and they don't have kits. Any help would be greatly appreciated. Thanks! Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From rjbuesa <@t> yahoo.com Mon Apr 27 11:07:25 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 27 11:07:28 2009 Subject: [Histonet] Embedding issues In-Reply-To: <44780C571F28624DBB446DE55C4D733A04257CFD@EXCHANGEBE1.carle.com> Message-ID: <977075.37787.qm@web65707.mail.ac4.yahoo.com> Sometimes screened cassettes have been blamed for defects in processing and to cause "bubbles" to?be maintained in the tissues surfaces. So?if that is the type of cassette your have narrowed your problem to that could be the cause. Ren? J. --- On Mon, 4/27/09, Sharon.Davis-Devine wrote: From: Sharon.Davis-Devine Subject: [Histonet] Embedding issues To: histonet@lists.utsouthwestern.edu Date: Monday, April 27, 2009, 11:07 AM We are having issues with our embedding. We are routinely seeing bubbles when we go to cut the blocks. Our embedders are very careful about tamping the tissue down to prevent this but it still seems to be happening. We have narrowed down to one particular cassette so that may be the problem. We were just wondering is anyone out there in Histoland has had similar problems and if you have how did you solve it? As always thanks for the help. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Mon Apr 27 11:10:05 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Mon Apr 27 11:10:07 2009 Subject: [Histonet] Fontana Masson In-Reply-To: Message-ID: <1075279596.60831240848605235.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Try Polyscientific R&D I think they have it. Pam Marcum UPENN Vet Sch New Bolton Center ----- Original Message ----- From: "Jodi Putnam" To: histonet@lists.utsouthwestern.edu Sent: Monday, April 27, 2009 12:03:11 PM GMT -05:00 US/Canada Eastern Subject: [Histonet] Fontana Masson Hello. Do any of you know of a source for a kit for the Fontana Masson? I am used to doing it on the bench and making up all the solutions, but under the circumstances, a kit might be better. I have checked Sigma and Newcomersupply so far and they don't have kits. Any help would be greatly appreciated. Thanks! Jodi ? Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpaveli1 <@t> hurleymc.com Mon Apr 27 11:28:30 2009 From: lpaveli1 <@t> hurleymc.com (Lynette Pavelich) Date: Mon Apr 27 11:28:41 2009 Subject: [Histonet] AMMONIUM HYDROXIDE Message-ID: <49F5A4EE020000EE000289BD@smtp-gw.hurleymc.com> We've used ammonium hydroxide on occasion with no problems. My experiences with wash off's have turned out to be processing issues. Lynette >>> Rene J Buesa 04/27/09 12:03 PM >>> If it was strong ("pure" or 28%) ammonium hydroxide, and the sections were not completely drained after placing them in the slides, that could be the cause. Ren? J. --- On Mon, 4/27/09, Phyllis Thaxton wrote: From: Phyllis Thaxton Subject: [Histonet] AMMONIUM HYDROXIDE To: Histonet@lists.utsouthwestern.edu Date: Monday, April 27, 2009, 10:02 AM Hi all, I looked in archives to see if I could find any info on this but did not. Remember, we are having a problem with some of the sections floating off during IHC. If paraffin blocks are faced and then placed in ammonium hydroxide before cutting the H&E, would this cause the sections to float off if the unstained sections that are cut in addition to the H&E's are also produced in this manner? Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PIXLEYSK <@t> UCMAIL.UC.EDU Mon Apr 27 11:54:14 2009 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Mon Apr 27 11:54:21 2009 Subject: [Histonet] long term storage for IHC In-Reply-To: <200904271610.LBK00242@mprelay1.uc.edu> References: <200904271610.LBK00242@mprelay1.uc.edu> Message-ID: Here is our method for long term storage for IHC when doing cryostat sectioning, a generous gift from a colleague who kept materials for many years, then did freezing and cryostat sectioning and antibody staining with no losses. Unfortunately, it does take refrigerator space. This was designed for animal tissues (brain) perfused with 4% paraformaldehyde. I might suggest a washing step(s) in phosphate buffer to go from formalin (which has some alcohol) to the cryoprotectant: 1. After fixing brain in paraformaldehyde, put directly (no washing) in cryoprotectant 2. Store in refrigerator for years. 3. Remove tissue and place in pure sucrose solution until tissue sinks, at least 24 hours. Can keep in sucrose 1 week or so. 4. Freeze and section on cryostat. Cryoprotectant: (30% ethylene glycol v/v; 30 % sucrose, 0.02% Na Azide, 0.04 M Phosphate Buffer) Total volume: 1 liter: Add, in order indicated: 1. 200 ml dd water 2. 200 ml 0.2 M phosphate buffer (PB) (final conc. 0.04M PB) 3. 300 ml Ethylene Glycol 4. 10 ml of 2% Na Azide 5. 300 g sucrose: add slowly (~100 g at a time) with stirring (if you use lower sucrose, make this lower also) 6. Stir until dissolved 7. Check volume, but should be 1 liter. 8. Refrigerate We used to embed our specimens in our cryomaterial (i.e. OCT), freeze them and kept them frozen in film canisters, similar to what another person suggested. However we kept them at -80 degrees C and we would put a few pieces of regular ice in to make sure they stayed hydrated. However, sometimes there were air leaks or cracks and the specimens would lose water ("freeze-dry"). As per another suggestion here, I would be wary of using parafilm because it cracks when frozen. This cryoprotectant method (above) is therefore better than these because of these problems, although the antigenicity is retained when you keep the blocks frozen. We do know that you CANNOT keep the frozen sections for very long, because they definitely lose antigenicity. Best, Sarah From azdudley <@t> hotmail.com Mon Apr 27 12:07:26 2009 From: azdudley <@t> hotmail.com (anita dudley) Date: Mon Apr 27 12:07:30 2009 Subject: [Histonet] ventana Message-ID: just wondering for those who have the benchmark, do you print out the reports for every run and have the dr.s sign? we were told the every line on the form needs to be signed. it is a lot of extra writing we feel and are trying to come up with a qa sheet for immuno, special stains and h&e all on one. does anyone have any suggestions? thanks anita providence hosp mobile alabama _________________________________________________________________ Windows Live? Hotmail?:?more than just e-mail. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_more_042009 From jqb7 <@t> cdc.gov Mon Apr 27 12:11:24 2009 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Apr 27 12:11:35 2009 Subject: [Histonet] Fontana Masson In-Reply-To: References: Message-ID: <9A16CB5D55FC1648ADF11B63E72A1BE101AD2F91@LTA3VS011.ees.hhs.gov> I love the one from American Mastertech. www.americanmastertech.com Product code: KTFMA Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Putnam, Jodi Sent: Monday, April 27, 2009 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fontana Masson Hello. Do any of you know of a source for a kit for the Fontana Masson? I am used to doing it on the bench and making up all the solutions, but under the circumstances, a kit might be better. I have checked Sigma and Newcomersupply so far and they don't have kits. Any help would be greatly appreciated. Thanks! Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mabosso <@t> unipathllc.com Mon Apr 27 12:15:04 2009 From: mabosso <@t> unipathllc.com (Mary Abosso) Date: Mon Apr 27 12:17:45 2009 Subject: [Histonet] Another CAP question Message-ID: <43A451981FF6634795BE83B1B5494D631BDFA5@exchange.unipathllc.corp> Good Monday all - We were just talking about this reg. and wondering how everyone is answering this? Are instrument maintenance records acceptable? ANP.23075 - Is there evidence of ongoing evaluation of results of instrument maintenance and function for all devices? Mary Abosso From portera <@t> msu.edu Mon Apr 27 12:27:54 2009 From: portera <@t> msu.edu (Amy Porter) Date: Mon Apr 27 12:28:00 2009 Subject: [Histonet] Another CAP question References: <43A451981FF6634795BE83B1B5494D631BDFA5@exchange.unipathllc.corp> Message-ID: I use my yearly p.m. records, certified themometer maintenance records, I concentrated more on the function item and not the ongoing results. In my mind if something is broken or repaired the ongoing results have not been satisfactory. Hope this helps - Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Mary Abosso" To: "Histonet" Sent: Monday, April 27, 2009 1:15 PM Subject: [Histonet] Another CAP question Good Monday all - We were just talking about this reg. and wondering how everyone is answering this? Are instrument maintenance records acceptable? ANP.23075 - Is there evidence of ongoing evaluation of results of instrument maintenance and function for all devices? Mary Abosso _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robinsoc <@t> mercyhealth.com Mon Apr 27 12:35:07 2009 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Mon Apr 27 12:36:41 2009 Subject: [Histonet] ventana In-Reply-To: References: Message-ID: <49F5A67C.59AC.00AF.0@mercyhealth.com> We print a worksheet for each pathologist who will be reading slides from the IHC run. If we had to circulate one sheet to get every line signed it would be problematic as we service two locations. We just were CAP inspected and it went great. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robinsoc@mercyhealth.com >>> anita dudley 04/27/2009 12:07 PM >>> just wondering for those who have the benchmark, do you print out the reports for every run and have the dr.s sign? we were told the every line on the form needs to be signed. it is a lot of extra writing we feel and are trying to come up with a qa sheet for immuno, special stains and h&e all on one. does anyone have any suggestions? thanks anita providence hosp mobile alabama _________________________________________________________________ Windows Live? Hotmail?:?more than just e-mail. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_more_042009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbarone <@t> NEMOURS.ORG Mon Apr 27 12:37:30 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Mon Apr 27 12:37:23 2009 Subject: [Histonet] VA mtg Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A71FE@wlmmsx01.nemours.org> Histo-netter's: Earn 5 CEU's in one Saturday at the beach!. Virginia Beach, Virgina. Hosted by the states of DE, MD, NJ and PA...this is not a Region II meeting, rather a meeting hosted by sister states to help the Virginia Society back up to it's feet...while you earn CEU's in one day....and spend Sunday at the Beach! To be held at the Old Dominion University Higher Education Center, on May 16th. Here are the topics: Antibody Panels in the Anatomic Laboratory - Tara Kennedy/Application Specialist and Multiplex Stains for the Anatomic Pathology Laboratory- Kathy Bowden/application Specialist. For more info, e-mail: cbarone@ nemours.org. From jlhowery <@t> yrmc.org Mon Apr 27 13:00:22 2009 From: jlhowery <@t> yrmc.org (jeff) Date: Mon Apr 27 13:00:32 2009 Subject: [Histonet] Cryo Decontam Message-ID: <000601c9c762$09de5d20$3394640a@yrmc.org> I know we have spoke of this before but I can't find it. We Decontam our cryostats every 6 months or if we have a granuloma. Now if you cut a good number of frozens should the cryostat be done more often. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From b-frederick <@t> northwestern.edu Mon Apr 27 13:22:45 2009 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Apr 27 13:23:01 2009 Subject: [Histonet] Fontana Masson In-Reply-To: Message-ID: <7E1104312B1343378374097997453248@lurie.northwestern.edu> Rowley biochemical may also have it. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Putnam, Jodi Sent: Monday, April 27, 2009 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fontana Masson Hello. Do any of you know of a source for a kit for the Fontana Masson? I am used to doing it on the bench and making up all the solutions, but under the circumstances, a kit might be better. I have checked Sigma and Newcomersupply so far and they don't have kits. Any help would be greatly appreciated. Thanks! Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MDiCarlo <@t> KaleidaHealth.Org Mon Apr 27 14:16:39 2009 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Mon Apr 27 14:16:51 2009 Subject: [Histonet] different microtome? Message-ID: <1B73766A27A1554CB2729B6432E81301015C69FE@KALEXMB04.KaleidaHealth.org> Hello Everyone, I am attempting to cut decaled (with EDTA) dog bone tissue which is attached to a partial tooth, manually processed and embedded in Tissue Prep 2 paraffin. I have cut one block but have only gotten thick and thin sections. I am currently using a Microm HM 325 using high profile disposable blades. My boss wanted me to find out if there is a better microtome out there that could cut this tissue serially. Are there any? Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 2008 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From mucram11 <@t> comcast.net Mon Apr 27 14:25:51 2009 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Mon Apr 27 14:25:55 2009 Subject: [Histonet] different microtome? In-Reply-To: <1B73766A27A1554CB2729B6432E81301015C69FE@KALEXMB04.KaleidaHealth.org> Message-ID: <1196899391.27621240860351739.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Peggy, We are cutting decal in paraffin and non-decal bone in MMA on Leica RM2255 microtomes almost daily.? The motor is strong enough for the MMA and we have great success using both manual and motorized sectioning for decal bone.? We do large pieces of sheep spine for both.? The sections have always been even for just picking up a few sections or serial sections.? Hope this helps. Pam Marcum UPENN Vet Sch New Bolton Center ----- Original Message ----- From: "Margaret DiCarlo" To: histonet@pathology.swmed.edu Sent: Monday, April 27, 2009 3:16:39 PM GMT -05:00 US/Canada Eastern Subject: [Histonet] different microtome? Hello Everyone, ? I am attempting to cut decaled (with EDTA) dog bone tissue which is attached to a partial tooth, manually processed and embedded in Tissue Prep 2 paraffin. ?I have cut one block but have only gotten thick and thin sections. ?I am currently using a Microm HM 325 using high profile disposable blades. ?My boss wanted me to find out if there is a better microtome out there that could cut this tissue serially. ?Are there any? ? Thank you. ? Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY ?14203 716-859-1293 ? 2008 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Apr 27 15:37:57 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 27 15:38:02 2009 Subject: [Histonet] RE: haemotol autostainer? In-Reply-To: <7722595275A4DD4FA225B92CDBF174A17457783D35@EXC-MBX3.cfs.le.ac.uk> Message-ID: <31984.9506.qm@web65703.mail.ac4.yahoo.com> These type of stainers are regularly used in the hematology sections of the med lab. There is where he can find some answers Ren? J. --- On Mon, 4/27/09, Edwards, R.E. wrote: From: Edwards, R.E. Subject: [Histonet] RE: haemotol autostainer? To: "Histonet" Date: Monday, April 27, 2009, 11:40 AM A colleague is looking to purchase a staining machine, primarily for Romanowsky type staining, any recommendations or brick bats please. Richard Edwards Leicester University...UK. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Mon Apr 27 15:43:14 2009 From: jcline <@t> wchsys.org (Joyce Cline) Date: Mon Apr 27 15:43:18 2009 Subject: [Histonet] Another CAP question In-Reply-To: <43A451981FF6634795BE83B1B5494D631BDFA5@exchange.unipathllc.corp> Message-ID: <2D16EE4265724552995975A3DC9EE232@wchsys.org> I review and sign each week to prove the maintenance and temperature records are checked. I was asked by an inspector about a few days that the temps were out of bounds for an oven. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Abosso Sent: Monday, April 27, 2009 1:15 PM To: Histonet Subject: [Histonet] Another CAP question Good Monday all - We were just talking about this reg. and wondering how everyone is answering this? Are instrument maintenance records acceptable? ANP.23075 - Is there evidence of ongoing evaluation of results of instrument maintenance and function for all devices? Mary Abosso _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From jcline <@t> wchsys.org Mon Apr 27 15:51:55 2009 From: jcline <@t> wchsys.org (Joyce Cline) Date: Mon Apr 27 15:52:03 2009 Subject: [Histonet] ventana In-Reply-To: Message-ID: <0D5A5E5E16A94BE2B16589C38851FF9A@wchsys.org> The technician signs the Ventana printout for the +/- on the controls for the IHC and the Specials. We have separate Quality control worksheets for the specials and the IHC that the pathologists sign. These worksheets have the case #, who cut/ran the stains, quality of cut/stain, and pathologist approval. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Monday, April 27, 2009 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ventana just wondering for those who have the benchmark, do you print out the reports for every run and have the dr.s sign? we were told the every line on the form needs to be signed. it is a lot of extra writing we feel and are trying to come up with a qa sheet for immuno, special stains and h&e all on one. does anyone have any suggestions? thanks anita providence hosp mobile alabama _________________________________________________________________ Windows LiveT HotmailR:.more than just e-mail. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_more_042009______ _________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From sccrshlly <@t> yahoo.com Mon Apr 27 16:16:04 2009 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Mon Apr 27 16:16:09 2009 Subject: [Histonet] Re: Embedding Issues Message-ID: <555911.53131.qm@web90306.mail.mud.yahoo.com> I am not sure if you are referring to bubble between the tissue and the bottom of the molds, or bubbles causing holes between the tissue and the cassette, causing the block to "collapse".? The previous would probably have something to do with the paraffin feeding into the block, perhaps there is a leak in the system causing air bubbles in the paraffin.? As for the latter, we have experienced this with the cassettes that we use, and have found that 3-4 good hard taps on the top of the cassette after filling with paraffin has helped.? Also, we do have to be sure that the initial fill of paraffin is just above the lip of the mold, so that when we set the cassette on top, there is a small amount of paraffin in the cassette already.? These two steps have all but eliminated the air bubbles between the tissue and the cassette (blocks collapsing).? ? Good luck and hope this helps, Shelly --- On Mon, 4/27/09, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 65, Issue 45 To: histonet@lists.utsouthwestern.edu Date: Monday, April 27, 2009, 1:57 PM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. help (Dertien, Janet) ???2. Re: help (Rene J Buesa) ???3. RE Finding Iron in Decalcified Sections (Farish, Craig) ???4. Doublecortin, Ki67, Caspase-3: need antigen retrieval ? (TF) ???5. RE Megacassettes (Farish, Craig) ???6. RE: Histonet Digest, Vol 65, Issue 44 (Stella_quan@DNAR.com) ???7. AMMONIUM HYDROXIDE (Phyllis Thaxton) ???8. RE: AMMONIUM HYDROXIDE (Sebree Linda A) ???9. RE: AMMONIUM HYDROXIDE (Bartlett, Jeanine (CDC/CCID/NCZVED)) ? 10. AW: [Histonet] help (Gudrun Lang) ? 11. HT exam (MaryAnn Dixon) ? 12. Re: HT exam (Pamela Marcum) ? 13. Sensitivity and Specificity of Antibodies for IHC (Jessica Piche) ? 14. Re: HT exam (stephanie.d.rivera@gsk.com) ? 15. Embedding issues (Sharon.Davis-Devine) ? 16. breast tissue (Caleri, Kathleen) ? 17. RE: haemotol autostainer? (Edwards, R.E.) ? 18. Re: AMMONIUM HYDROXIDE (Rene J Buesa) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Mon Apr 27 14:04:26 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Mon Apr 27 16:24:04 2009 Subject: [Histonet] Cracked or shattered LN sections Message-ID: Hi Folks, Some lymph node sections (usually bigger ones-maybe almost as big as a dime) have a shattered appearance on the block surface. Subsequently the sections look like shattered glass or a mosaic of tissue pieces making up the whole. I think this is happening at the embedding station from trying to make sure that the tissue is flat in the mold (ie pressing the tissue down). Has anyone else out there have any similar experience with this problem? Is the solution as simple as cutting smaller pieces into the cassette at the time of trimming? Thanks. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From peddinti_2002us <@t> yahoo.co.in Mon Apr 27 16:55:42 2009 From: peddinti_2002us <@t> yahoo.co.in (kamal prasad) Date: Mon Apr 27 16:55:50 2009 Subject: [Histonet] Zheil nelson stain Message-ID: <924360.55013.qm@web95212.mail.in2.yahoo.com> Dear Janet, ? Are you heating the sections during corbol fuchsin incubation? What percentage of decolorizer are you using? You can use?either 3%Acid alcohol or 1%HCL. Please check under microscope wether its properly decolorized or not, then add methylene blue or malachite green as counter stain. ? Regards, Kamal Now surf faster and smarter ! Check out the new Firefox 3 - Yahoo! Edition http://downloads.yahoo.com/in/firefox/?fr=om_email_firefox From LSebree <@t> uwhealth.org Mon Apr 27 17:36:12 2009 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Apr 27 17:38:14 2009 Subject: [Histonet] Cracked or shattered LN sections References: Message-ID: <5998F3BDFF7AAC4091C7AE93A7A1A58931A9ED@UWHC-MAIL01.uwhis.hosp.wisc.edu> Greg, Are you sure no one is using freezing spray when sectioning? The effect of freezing spray on the block would yield the appearance you describe. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Greg Dobbin Sent: Mon 4/27/2009 2:04 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cracked or shattered LN sections Hi Folks, Some lymph node sections (usually bigger ones-maybe almost as big as a dime) have a shattered appearance on the block surface. Subsequently the sections look like shattered glass or a mosaic of tissue pieces making up the whole. I think this is happening at the embedding station from trying to make sure that the tissue is flat in the mold (ie pressing the tissue down). Has anyone else out there have any similar experience with this problem? Is the solution as simple as cutting smaller pieces into the cassette at the time of trimming? Thanks. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Apr 27 18:00:07 2009 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Apr 27 18:00:19 2009 Subject: [Histonet] processing tissues with silicone Message-ID: <22ED851776B7471AAC9AA368273D013D@Patsyoffice> Does anyone have experience processing tissues that have silicone in them. We assume the silicone will be dissolved by paraffin processing? I suggested using xylene substitute on the processor (I use this for processing cells on plastic constructs and it works well) or perhaps glycol methacrylate embedding which does not need to use xylene. Any ideas? Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From dogsloveus2 <@t> yahoo.com Mon Apr 27 20:25:26 2009 From: dogsloveus2 <@t> yahoo.com (Tara Manierre) Date: Mon Apr 27 20:25:31 2009 Subject: [Histonet] Post Please Message-ID: <770646.70020.qm@web59908.mail.ac4.yahoo.com> Hi, I am an ASCP certified Histotechnologist with over eight years of experience.? Relocating in May to Des Moines, Iowa.? Does anyone know of any openings in Des Moines or surrounding areas?? Any help would be greatly appreciated! Thanks very much! TJ? dogsloveus2@yahoo.com From nicholasprosenbaum <@t> yahoo.com Mon Apr 27 20:26:11 2009 From: nicholasprosenbaum <@t> yahoo.com (Nicholas Rosenbaum) Date: Mon Apr 27 20:26:15 2009 Subject: [Histonet] Fontana Masson In-Reply-To: References: Message-ID: <362413.95988.qm@web35205.mail.mud.yahoo.com> Try Poly Scientific Nicholas Rosenbaum HT, ASCP University of Virginia Medical Center ________________________________ From: "Putnam, Jodi" To: histonet@lists.utsouthwestern.edu Sent: Monday, April 27, 2009 12:03:11 PM Subject: [Histonet] Fontana Masson Hello. Do any of you know of a source for a kit for the Fontana Masson? I am used to doing it on the bench and making up all the solutions, but under the circumstances, a kit might be better. I have checked Sigma and Newcomersupply so far and they don't have kits. Any help would be greatly appreciated. Thanks! Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Mon Apr 27 21:26:53 2009 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Mon Apr 27 21:27:14 2009 Subject: [Histonet] Cracked or shattered LN sections Message-ID: I am positive no freezing spray being used. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Sebree Linda A" 04/27/09 7:36 PM >>> Greg, Are you sure no one is using freezing spray when sectioning? The effect of freezing spray on the block would yield the appearance you describe. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Greg Dobbin Sent: Mon 4/27/2009 2:04 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cracked or shattered LN sections Hi Folks, Some lymph node sections (usually bigger ones-maybe almost as big as a dime) have a shattered appearance on the block surface. Subsequently the sections look like shattered glass or a mosaic of tissue pieces making up the whole. I think this is happening at the embedding station from trying to make sure that the tissue is flat in the mold (ie pressing the tissue down). Has anyone else out there have any similar experience with this problem? Is the solution as simple as cutting smaller pieces into the cassette at the time of trimming? Thanks. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From kdwyer3322 <@t> aol.com Mon Apr 27 21:26:54 2009 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Mon Apr 27 21:27:37 2009 Subject: [Histonet] Texas Society for Histotechnology Symposium Convention May 29-31, 2009 -Austin,Texas Message-ID: <8CB95BFEE836785-1134-2374@FWM-M16.sysops.aol.com> Histonetters! It's not too late to sign up for the Texas Society for Histotechnology meeting.? Pre registration for the meeting is still available!!??Rooms at the hotel are filling up fast so make your hotel reservations as soon as possible.? The Symposium/Convention is?in beautiful Austin,Texas May 29-31, 2009 (not Memorial Day weekend).? The Convention will be held at the Hyatt Regency, 208 Barton Springs Road, Austin,Texas.? Please join us for a great "Texas Style" meeting.? All 16 workshops are contact hour approved by NSH. If you are a vendor and want to exhibit at the meeting we still have a few available booths.? Contact Becki Ruser at histobr@yahoo.com. For more information or a e-mail copy of the program contact: Veronida@baylorhealth.edu or kdywer3322@aol.com or www.txsh.org From kdwyer3322 <@t> aol.com Mon Apr 27 21:26:54 2009 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Mon Apr 27 21:27:42 2009 Subject: [Histonet] Texas Society for Histotechnology Symposium Convention May 29-31, 2009 -Austin,Texas Message-ID: <8CB95BFEE836785-1134-2374@FWM-M16.sysops.aol.com> Histonetters! It's not too late to sign up for the Texas Society for Histotechnology meeting.? Pre registration for the meeting is still available!!??Rooms at the hotel are filling up fast so make your hotel reservations as soon as possible.? The Symposium/Convention is?in beautiful Austin,Texas May 29-31, 2009 (not Memorial Day weekend).? The Convention will be held at the Hyatt Regency, 208 Barton Springs Road, Austin,Texas.? Please join us for a great "Texas Style" meeting.? All 16 workshops are contact hour approved by NSH. If you are a vendor and want to exhibit at the meeting we still have a few available booths.? Contact Becki Ruser at histobr@yahoo.com. For more information or a e-mail copy of the program contact: Veronida@baylorhealth.edu or kdywer3322@aol.com or www.txsh.org From nicholasprosenbaum <@t> yahoo.com Mon Apr 27 22:02:12 2009 From: nicholasprosenbaum <@t> yahoo.com (Nicholas Rosenbaum) Date: Mon Apr 27 22:02:18 2009 Subject: [Histonet] Cracked or shattered LN sections In-Reply-To: References: Message-ID: <194735.9037.qm@web35207.mail.mud.yahoo.com> How thick are you cutting your lymph nodes? Nicholas Rosenbaum HT, ASCP University of Virginia Medical Center ________________________________ From: Greg Dobbin To: Histonet@lists.utsouthwestern.edu; LSebree@uwhealth.org Sent: Monday, April 27, 2009 10:26:53 PM Subject: RE: [Histonet] Cracked or shattered LN sections I am positive no freezing spray being used. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson >>> "Sebree Linda A" 04/27/09 7:36 PM >>> Greg, Are you sure no one is using freezing spray when sectioning? The effect of freezing spray on the block would yield the appearance you describe. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Greg Dobbin Sent: Mon 4/27/2009 2:04 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cracked or shattered LN sections Hi Folks, Some lymph node sections (usually bigger ones-maybe almost as big as a dime) have a shattered appearance on the block surface. Subsequently the sections look like shattered glass or a mosaic of tissue pieces making up the whole. I think this is happening at the embedding station from trying to make sure that the tissue is flat in the mold (ie pressing the tissue down). Has anyone else out there have any similar experience with this problem? Is the solution as simple as cutting smaller pieces into the cassette at the time of trimming? Thanks. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dogsloveus2 <@t> yahoo.com Mon Apr 27 21:26:47 2009 From: dogsloveus2 <@t> yahoo.com (Tara Manierre) Date: Mon Apr 27 22:21:31 2009 Subject: [Histonet] Posting Message-ID: <66425.90834.qm@web59902.mail.ac4.yahoo.com> Hi, Could you please remove my post regarding Des Moines?? Sorry.... causing problems for me. Thank you! Tara From rjbuesa <@t> yahoo.com Tue Apr 28 08:32:23 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 28 08:32:28 2009 Subject: [Histonet] Cracked or shattered LN sections Message-ID: <874859.56754.qm@web65713.mail.ac4.yahoo.com> Shattering is not produced at the embedding section. It is caused during processing specially because of improper dehydration. If the LN are too thick to begin with, it will also contribute to improper dehydration and as a consequence poor infiltration. Ren? J. --- On Mon, 4/27/09, Greg Dobbin wrote: From: Greg Dobbin Subject: [Histonet] Cracked or shattered LN sections To: Histonet@lists.utsouthwestern.edu Date: Monday, April 27, 2009, 3:04 PM Hi Folks, Some lymph node sections (usually bigger ones-maybe almost as big as a dime) have a shattered appearance on the block surface. Subsequently the sections look like shattered glass or a mosaic of tissue pieces making up the whole. I think this is happening at the embedding station from trying to make sure that the tissue is flat in the mold (ie pressing the tissue down). Has anyone else out there have any similar experience with this problem? Is the solution as simple as cutting smaller pieces into the cassette at the time of trimming? Thanks. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "I find that the harder I work, the more luck I seem to have." - Thomas Jefferson ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kalschev <@t> svm.vetmed.wisc.edu Tue Apr 28 09:01:50 2009 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Tue Apr 28 09:03:38 2009 Subject: [Histonet] canine bone, EDTA Message-ID: <002001c9c809$e0650ef0$c5d76880@vetmed.wisc.edu> Peggy, If your microtome will hold a sharp D profile or a tungsten carbide blade, you might be able to get consistent sections. I find that EDTA decalcification hardens the tissue ( as if it wasn't hard enough already). Also, extending the times for the paraffin infiltration is helpful and soaking the block in icy/soapy detergent in the freezer works on some samples. ~ VIcki From AWempren <@t> skcc.org Tue Apr 28 11:03:21 2009 From: AWempren <@t> skcc.org (Alexina Wempren) Date: Tue Apr 28 11:03:58 2009 Subject: [Histonet] Alkaline Phos. Message-ID: <8A4D3B8263530D4E8D00159CE84F352844DB4C1C48@mail.skcc.org> Hi all, Is there a way to knock out endogenous Alk. Phos? Thanks From ian.montgomery <@t> bio.gla.ac.uk Tue Apr 28 11:33:32 2009 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Tue Apr 28 11:35:29 2009 Subject: FW: [Histonet] Alkaline Phos. Message-ID: <531C533F9EF44F9D9B46045D14798595@IBLS.GLA.AC.UK> Quabain. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alexina Wempren Sent: 28 April 2009 17:03 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Alkaline Phos. Hi all, Is there a way to knock out endogenous Alk. Phos? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From n.singh <@t> biogenex.com Tue Apr 28 12:15:40 2009 From: n.singh <@t> biogenex.com (Neeraj K. Singh) Date: Tue Apr 28 12:18:12 2009 Subject: [Histonet] (no subject) Message-ID: <37DC9F93CF7F864182D0463EF93D571B999366@ISLETON2.california.biogenex.com> Hi, You can use Levamisole to block endogenous Alk. phosphatase. Block it for 10-15 mins. Thanks Neeraj From kmerriam2003 <@t> yahoo.com Tue Apr 28 13:11:42 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Apr 28 13:11:46 2009 Subject: [Histonet] firefly luciferase Message-ID: <610310.7738.qm@web50302.mail.re2.yahoo.com> Hi, Is anyone doing firefly luciferase staining in FFPE mouse tissues?? Any antibody recommendations? Thanks, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From kgrobert <@t> rci.rutgers.edu Tue Apr 28 14:29:48 2009 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Tue Apr 28 14:16:31 2009 Subject: [Histonet] Destaining H&E slides Message-ID: <49F7592C.3070307@rci.rutgers.edu> I searched the archives and found Gary Gill's method for destaining H&E slides, and for destaining eosin he calls for 1.5% NH4OH in 70% ethanol....the problem is, I don't have any NH4OH in the lab. Is there another way to fully destain eosin? Thanks, Kathleen Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854 (732) 445-6914 FAX (732) 445-6905 From tammy <@t> surgicalpathlabs.com Tue Apr 28 14:07:53 2009 From: tammy <@t> surgicalpathlabs.com (Tammy de Leon) Date: Tue Apr 28 14:35:54 2009 Subject: [Histonet] Florida Histo Openings Message-ID: We are a Pathology Lab in Pinellas Park, Florida. We're looking to add to our team! Candidates should be an HTL or HT (ASCP) or equivalent, have a valid driver's license and proof of insurability. Primary responsibilities include on site frozen sections including mobile laboratory units. We offer a full benefits package (SPL pays 100% of the premium for Medical, Dental, Life and LTD) with a great working environment. View our Company Info and job details at www.surgicalpathlabs.com. Thanks. Tammy R. de Leon Surgical Pathology Laboratories Human Resources Officer 8455 66th Street N Pinellas Park, Fl 33781 Office Phone - 727-209-1215 Fax - 727-545-1644 From sfeher <@t> CMC-NH.ORG Tue Apr 28 14:40:14 2009 From: sfeher <@t> CMC-NH.ORG (Feher, Stephen) Date: Tue Apr 28 14:42:35 2009 Subject: [Histonet] Floor materials Message-ID: <73A7ED895EE0C24D9267ED814911DF190E13B587@exchange.cmc-nh.org> We're building a pathology lab and we're at project phase where flooring materials is being discussed. The initial choice of the architect is to use sheet vinyl flooring in the working areas of the lab (with the exception of the morgue). I'm not particularly impressed with the way vinyl flooring stands up to stains, solvents and wax. Do any of you have suggestions or experience in a type of flooring for the path lab that is superior to commercial vinyl? Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org From rjbuesa <@t> yahoo.com Tue Apr 28 15:10:08 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 28 15:10:12 2009 Subject: [Histonet] Destaining H&E slides In-Reply-To: <49F7592C.3070307@rci.rutgers.edu> Message-ID: <975537.69357.qm@web65701.mail.ac4.yahoo.com> You can destained eosin with diluted (1:20) bleach. Ren? J. --- On Tue, 4/28/09, Kathleen Roberts wrote: From: Kathleen Roberts Subject: [Histonet] Destaining H&E slides To: "'histonet@lists.utsouthwestern.edu'" Date: Tuesday, April 28, 2009, 3:29 PM I searched the archives and found Gary Gill's method for destaining H&E slides, and for destaining eosin he calls for 1.5% NH4OH in 70% ethanol....the problem is, I don't have any NH4OH in the lab. Is there another way to fully destain eosin? Thanks, Kathleen Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854 (732) 445-6914 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Apr 28 15:44:51 2009 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Apr 28 15:44:56 2009 Subject: [Histonet] Floor materials In-Reply-To: <73A7ED895EE0C24D9267ED814911DF190E13B587@exchange.cmc-nh.org> Message-ID: <338214.96621.qm@web65713.mail.ac4.yahoo.com> We used to have a cement floor and anti slipping mats that were changed weekly by a company that returned them to us when cleaned and dewaxed. All slipping problems were solved this way. The lab should have mats, regardless of the surface. Ren? J. --- On Tue, 4/28/09, Feher, Stephen wrote: From: Feher, Stephen Subject: [Histonet] Floor materials To: histonet@lists.utsouthwestern.edu Date: Tuesday, April 28, 2009, 3:40 PM We're building a pathology lab and we're at project phase where flooring materials is being discussed. The initial choice of the architect is to use sheet vinyl flooring in the working areas of the lab (with the exception of the morgue). I'm not particularly impressed with the way vinyl flooring stands up to stains, solvents and wax. Do any of you have suggestions or experience in a type of flooring for the path lab that is superior to commercial vinyl? Thanks, Steve Stephen A. Feher, MS, SCT (ASCP) Pathology Supervisor Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6707 sfeher@cmc-nh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dearolf <@t> hendrix.edu Tue Apr 28 17:52:23 2009 From: Dearolf <@t> hendrix.edu (Dearolf, Jennifer) Date: Tue Apr 28 17:52:27 2009 Subject: [Histonet] Rolling sections Message-ID: <9F3B003643FC5F42930EFB83E276C8608B02EA7CE0@HNXEXCH.hendrix.local> Greetings, Histonetters! First, I wanted to thank all of you that responded to my e-mail a few years back about freezing small pieces of muscle tissue. We have found a method that works for us, and if anyone is interested, I would be happy to share. It still involves the wonderfully explosive isopentane, but it allows us to freeze fetal guinea pig muscle without artifact. I am writing today to ask a question about cutting frozen sections with a cryostat. We are having problems with the sections rolling once they come off the knife and before we can get them on a slide. We have a Microm 505E cryostat, and we cut our OCT mounted specimens at around -25 degrees C. We use Accuedge high profile blades, cut sections between 8 and 12 microns thick, and use a brush to pull the sections off. But, when we remove the brush, the sections roll up. Sometimes, they just arc up and other times they completely roll into a jellyroll. I have tried putting 70% EtOH in a beaker in the cryostat. This method was suggested to us by a vendor, but it doesn't seem to work consistently. We can also flatten the sections with a brush, but unless we are really quick, the sections roll up before we can get them on the slide. It makes it difficult to get serial sections. Any advice would be appreciated. Thanks again for all your help so far. Sincerely, Jenn Jennifer Dearolf, Ph.D. Associate Professor Biology Department Hendrix College 1600 Washington Ave. Conway, AR 72032 (501) 450-4530 (office) From becky.garrison <@t> jax.ufl.edu Tue Apr 28 18:28:43 2009 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Tue Apr 28 18:28:49 2009 Subject: [Histonet] Rolling sections In-Reply-To: <9F3B003643FC5F42930EFB83E276C8608B02EA7CE0@HNXEXCH.hendrix.local> References: <9F3B003643FC5F42930EFB83E276C8608B02EA7CE0@HNXEXCH.hendrix.local> Message-ID: Are you freezing muscle? If so, for our muscle biopsies we use OCT to adhere the base of the tissue to a piece of cork (which is frozen in the isopentane); the tissue is not surrounded by OCT. The tissue section is guided onto the blade holder with a brush and then picked up on the slide. All our other tissues are frozen surrounded by OCT. Becky Garrison Shands Jacksonville 904-244-6237 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dearolf, Jennifer Sent: Tuesday, April 28, 2009 6:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rolling sections Greetings, Histonetters! First, I wanted to thank all of you that responded to my e-mail a few years back about freezing small pieces of muscle tissue. We have found a method that works for us, and if anyone is interested, I would be happy to share. It still involves the wonderfully explosive isopentane, but it allows us to freeze fetal guinea pig muscle without artifact. I am writing today to ask a question about cutting frozen sections with a cryostat. We are having problems with the sections rolling once they come off the knife and before we can get them on a slide. We have a Microm 505E cryostat, and we cut our OCT mounted specimens at around -25 degrees C. We use Accuedge high profile blades, cut sections between 8 and 12 microns thick, and use a brush to pull the sections off. But, when we remove the brush, the sections roll up. Sometimes, they just arc up and other times they completely roll into a jellyroll. I have tried putting 70% EtOH in a beaker in the cryostat. This method was suggested to us by a vendor, but it doesn't seem to work consistently. We can also flatten the sections with a brush, but unless we are really quick, the sections roll up before we can get them on the slide. It makes it difficult to get serial sections. Any advice would be appreciated. Thanks again for all your help so far. Sincerely, Jenn Jennifer Dearolf, Ph.D. Associate Professor Biology Department Hendrix College 1600 Washington Ave. Conway, AR 72032 (501) 450-4530 (office) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Tue Apr 28 22:00:31 2009 From: tifei <@t> foxmail.com (TF) Date: Tue Apr 28 22:01:11 2009 Subject: [Histonet] Rolling sections References: <9F3B003643FC5F42930EFB83E276C8608B02EA7CE0@HNXEXCH.hendrix.local> Message-ID: <200904291100263073097@foxmail.com> SSBhbHNvIGhhdmUgcHJvYmxlbXMgaW4gbW91bnRpbmcgYnJhaW4gc2VjdGlvbnMuLi4xMHVtLTMw dW0gaW4gTGVpY2EgQ3J5b3N0YXQuLi4uaG9wZSBhbnlvbmUgY2FuIHNoYXJlIHRoZWlyIGV4cGVy aWVuY2U/DQoNCnNvbWV0aW1lcyB3ZSB1c2UgYSBicnVzaCB0byBwYWludCBhIG1pbmkgZHJvcCBv ZiBQQi93YXRlciBvbiB0byB0aGUgc2xpZGUsIGFuZCB0aGVuIG1vdW50IHRoZSBzZWN0aW9ucy4g aXQgaXMgdmVyeSBmbGF0IHRoZW4uDQoNCg0KMjAwOS0wNC0yOSANCg0KDQoNClRGIA0KDQoNCg0K 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b3IgYWxsIHlvdXIgaGVscCBzbyBmYXIuDQpTaW5jZXJlbHksDQpKZW5uDQpKZW5uaWZlciBEZWFy b2xmLCBQaC5ELg0KQXNzb2NpYXRlIFByb2Zlc3Nvcg0KQmlvbG9neSBEZXBhcnRtZW50DQpIZW5k cml4IENvbGxlZ2UgDQoxNjAwIFdhc2hpbmd0b24gQXZlLg0KQ29ud2F5LCBBUiA3MjAzMg0KKDUw MSkgNDUwLTQ1MzAgKG9mZmljZSkNCl9fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19f X19fX19fX19fX19fDQpIaXN0b25ldCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxpc3RzLnV0c291 dGh3ZXN0ZXJuLmVkdQ0KaHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xp c3RpbmZvL2hpc3RvbmV0DQo= From tifei <@t> foxmail.com Tue Apr 28 22:03:23 2009 From: tifei <@t> foxmail.com (TF) Date: Tue Apr 28 22:03:52 2009 Subject: [Histonet] BSA blocking and Goat-serum only blocking Message-ID: <200904291103184699514@foxmail.com> Hi all i want to ask a question about blocking in IHC I sometimes use 1% BSA + 2-10% goat serum blocking for goat-sourced secondary antibody I also tried only 10% goat serum blocking. it seems that the later method sometimes worked quite well, but i also observed some more background in recent days any comment is appreciated 2009-04-29 TF From ooi.ting.huay <@t> nhc.com.sg Wed Apr 29 00:14:20 2009 From: ooi.ting.huay <@t> nhc.com.sg (ooi.ting.huay@nhc.com.sg) Date: Wed Apr 29 00:15:47 2009 Subject: [Histonet] Rolling sections In-Reply-To: <200904291100263073097@foxmail.com> Message-ID: Tm9ybWFsbHksIHdlIHdpbGwgdXNlIGJydXNoIHRvIGhvbGQgdGhlIHNlY3Rpb24gYWZ0ZXIgd2Ug aGF2ZSBzZWN0aW9uZWQgDQpvbmUgdGhpcmQgb2YgdGhlIHNhbXBsZS4gQWZ0ZXIgdGhhdCwgd2Ug d2lsbCBjb250aW51ZSBzZWN0aW9uIHRoZSBzYW1wbGUgDQp1bnRpbGwgdGhlIGVuZC4gSWYgdGhl IHNhbXBsZSBpcyByb2xsZWQgdXAsIHdlIHVzZSB0aGUgYnJ1c2ggdG8gc21vb3RoZW4gDQppdC4g VGhlbiBxdWlja2x5IGZsaXAgdGhlIGdsYXNzIHNsaWRlIHRvIGZpc2ggdGhlIHNhbXBsZS4gQXMg dGhlIGdsYXNzIA0Kc2xpZGUgaXMga2VlcCBpbiByb29tIHRlbXBlcmF0dXJlICh3YXJtKSwgd2hl biBwdXQgaW5zaWRlIHRoZSBjcnlvc3RhdCANCm1hY2hpbmUgKGluc2lkZSBpcyBjb2xkKSwgdGhl IHNlY3Rpb24gd2lsbCBqdXN0IHN0aWNrIHRvIHRoZSBnbGFzcyBzbGlkZS4gDQpUaGlzIHdvcmtz IHF1aXRlIHdlbGwgaW4gb3VyIGxhYi4gSG9wZSB0aGlzIGNhbiBoZWxwLi4uDQoNCg0KDQpSZWdh 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Mounting sections, rolling sections and peeling off sections Message-ID: <200904291430585165549@foxmail.com> WWVzIHRoaXMgaXMgYSB2ZXJ5IGNvbW1vbiBhcHByb2FjaC4uLg0Kd2UgZXhwZXJpZW5jZSBkaWZm aWN1bHR5IHBhcnRseSBiZWNhdXNlIHdlIGFyZSBjdXR0aW5nIHRoaW4gc2VjdGlvbnMgb24gbGFy Z2Ugc2FtcGxlcyBmcm9tIHdvdW5kZWQgdGlzc3VlLi4uDQp0aGUgd291bmRlZCB0aXNzdWUgaXMg YSBiaXQgbW9yZSBmcmFnaWxlOyBhbHNvLCBpZiB0aGUgc2VjdGlvbiBpcyBsYXJnZSwgaXQgaXMg dmVyeSBoYXJkIHRvIHVzZSB0aGUgc21hbGwgYnJ1c2ggdG8gaG9sZCBpdCBkdXJpbmcgY3V0dGlu Zy4uLg0KDQppbiB0aGUgc2Vuc2Ugb2YgYnJ1c2hpbmcgc29tZSB3YXRlciBvciBQQiAodmVyeSB0 aW55IGFtb3VudCksIGkgd291bGQgbGlrZSB0byBhc2sgdGhlIHF1ZXN0aW9uIHRoYXQgaG93IGNh biBzZWN0aW9ucyBzdGljayB0byBzbGlkZXM/DQpXaWxsIHRoaXMgd2F0ZXIgbWVtYnJhbmUgKGFm dGVyIHlvdSBoYXZlIG1vdW50ZWQgc2VjdGlvbnMsIGl0IHNob3VsZCBiZSBhYnNvcmJlZCBieSB0 aGUgc2VjdGlvbikgY2F1c2VzIHNlY3Rpb25zIHRvIHBlZWwgb2ZmIGR1cmluZyBJSEMgb3Igb3Ro ZXIgc3RhaW5pbmcgcHJvY2VkdXJlcz8gSXMgdGhlIHdhcm0gdGVtcGVyYXR1cmUgb2YgYSBzbGlk 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IEF2ZS4NCkNvbndheSwgQVIgNzIwMzINCig1MDEpIDQ1MC00NTMwIChvZmZpY2UpDQpfX19fX19f X19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fXw0KSGlzdG9uZXQgbWFpbGlu ZyBsaXN0DQpIaXN0b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUNCmh0dHA6Ly9saXN0cy51 dHNvdXRod2VzdGVybi5lZHUvbWFpbG1hbi9saXN0aW5mby9oaXN0b25ldA0KX19fX19fX19fX19f X19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX18NCkhpc3RvbmV0IG1haWxpbmcgbGlz dA0KSGlzdG9uZXRAbGlzdHMudXRzb3V0aHdlc3Rlcm4uZWR1DQpodHRwOi8vbGlzdHMudXRzb3V0 aHdlc3Rlcm4uZWR1L21haWxtYW4vbGlzdGluZm8vaGlzdG9uZXQNCg0KDQpfX19fX19fX19fX19f X19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fIA0KQ29uZmlkZW50aWFsIGluZm9y bWF0aW9uLiBVbmF1dGhvcml6ZWQgdXNlIG9yIGRpc2Nsb3N1cmUgcHJvaGliaXRlZC4NClJlZmVy IGh0dHA6Ly93d3cuc2luZ2hlYWx0aC5jb20uc2cvQ29udGFjdFVzLyNEaXNjbGFpbWVyIA0K From louise.renton <@t> gmail.com Wed Apr 29 02:10:05 2009 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Apr 29 02:10:12 2009 Subject: [Histonet] Floor materials In-Reply-To: <73A7ED895EE0C24D9267ED814911DF190E13B587@exchange.cmc-nh.org> References: <73A7ED895EE0C24D9267ED814911DF190E13B587@exchange.cmc-nh.org> Message-ID: Vinyl floors can be almost lethally slippery if wax gets on them.... its ok if staff wear flat shoes but woe betide someone coming in with heels! On 4/28/09, Feher, Stephen wrote: > > We're building a pathology lab and we're at project phase where flooring > materials is being discussed. The initial choice of the architect is to > use sheet vinyl flooring in the working areas of the lab (with the > exception of the morgue). I'm not particularly impressed with the way > vinyl flooring stands up to stains, solvents and wax. Do any of you > have suggestions or experience in a type of flooring for the path lab > that is superior to commercial vinyl? > > Thanks, > > Steve > > > Stephen A. Feher, MS, SCT (ASCP) > > Pathology Supervisor > > Catholic Medical Center > > 100 McGregor Street > > Manchester, NH 03102 > > 603-663-6707 > > sfeher@cmc-nh.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From adam_anthony <@t> mba.berkeley.edu Wed Apr 29 02:22:19 2009 From: adam_anthony <@t> mba.berkeley.edu (Adam Anthony) Date: Wed Apr 29 02:23:43 2009 Subject: [Histonet] UC Berkeley students in need of help In-Reply-To: <671FB8AFBC3FD548A2CA262D71937E6A01596DB779B3@EXMAIL7.haas.uc.berkeley.edu> References: <671FB8AFBC3FD548A2CA262D71937E6A01596DB779B3@EXMAIL7.haas.uc.berkeley.edu> Message-ID: <671FB8AFBC3FD548A2CA262D71937E6A01596DB779C5@EXMAIL7.haas.uc.berkeley.edu> Fellow Histoneters, Can you spare less 2 minutes of your time to help out a group of 4 MBA students at UC Berkeley? We are conducting market research, by way of a simple online survey, for a class project. Our focus is on ways in which labs can cut significant costs on reagents and materials. There are 11 short questions- please help if you have time. Link to online survey: http://www.surveymonkey.com/s.aspx?sm=X3ssptgNq4oh7o0vwy1gCg_3d_3d Please contact me with any questions. Thanks, Adam Anthony adam_anthony@mba.berkeley.edu From kmerriam2003 <@t> yahoo.com Wed Apr 29 06:10:22 2009 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Apr 29 06:10:26 2009 Subject: [Histonet] Floor materials In-Reply-To: References: <73A7ED895EE0C24D9267ED814911DF190E13B587@exchange.cmc-nh.org> Message-ID: <833136.54832.qm@web50308.mail.re2.yahoo.com> I know they are expensive, but I think that epoxy floors are best in any kind of lab that uses solvents. ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: louise renton To: Histonet@lists.utsouthwestern.edu Sent: Wednesday, April 29, 2009 3:10:05 AM Subject: Re: [Histonet] Floor materials Vinyl floors can be almost lethally slippery if wax gets on them.... its ok if staff wear flat shoes but woe betide someone coming in with heels! On 4/28/09, Feher, Stephen wrote: > > We're building a pathology lab and we're at project phase where flooring > materials is being discussed.? The initial choice of the architect is to > use sheet vinyl flooring in the working areas of the lab (with the > exception of the morgue).? I'm not particularly impressed with the way > vinyl flooring stands up to stains, solvents and wax.? Do any of you > have suggestions or experience in a type of flooring for the path lab > that is superior to commercial vinyl? > > Thanks, > > Steve > > > Stephen A. Feher, MS, SCT (ASCP) > > Pathology Supervisor > > Catholic Medical Center > > 100 McGregor Street > > Manchester, NH 03102 > > 603-663-6707 > > sfeher@cmc-nh.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Wed Apr 29 08:04:15 2009 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Wed Apr 29 08:07:02 2009 Subject: [Histonet] Rolling sections In-Reply-To: <9F3B003643FC5F42930EFB83E276C8608B02EA7CE0@HNXEXCH.hendrix.local> References: <9F3B003643FC5F42930EFB83E276C8608B02EA7CE0@HNXEXCH.hendrix.local> Message-ID: <2A582E8156B45F468A62D1F1D20AF0837E5E6C@EX-BE08.ohsu.edu> Jennifer, It sounds like the sections are a little thick. Have you tried just barely blowing on the tissue with your breath to warm the OCT? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dearolf, Jennifer Sent: Tuesday, April 28, 2009 3:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rolling sections Greetings, Histonetters! First, I wanted to thank all of you that responded to my e-mail a few years back about freezing small pieces of muscle tissue. We have found a method that works for us, and if anyone is interested, I would be happy to share. It still involves the wonderfully explosive isopentane, but it allows us to freeze fetal guinea pig muscle without artifact. I am writing today to ask a question about cutting frozen sections with a cryostat. We are having problems with the sections rolling once they come off the knife and before we can get them on a slide. We have a Microm 505E cryostat, and we cut our OCT mounted specimens at around -25 degrees C. We use Accuedge high profile blades, cut sections between 8 and 12 microns thick, and use a brush to pull the sections off. But, when we remove the brush, the sections roll up. Sometimes, they just arc up and other times they completely roll into a jellyroll. I have tried putting 70% EtOH in a beaker in the cryostat. This method was suggested to us by a vendor, but it doesn't seem to work consistently. We can also flatten the sections with a brush, but unless we are really quick, the sections roll up before we can get them on the slide. It makes it difficult to get serial sections. Any advice would be appreciated. Thanks again for all your help so far. Sincerely, Jenn Jennifer Dearolf, Ph.D. Associate Professor Biology Department Hendrix College 1600 Washington Ave. Conway, AR 72032 (501) 450-4530 (office) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Wed Apr 29 08:36:11 2009 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Apr 29 08:44:59 2009 Subject: [Histonet] Destaining H&E slides In-Reply-To: <49F7592C.3070307@rci.rutgers.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835CBA@LSRIEXCH1.lsmaster.lifespan.org> Anything mildly basic will quickly destain eosin. Running tap water will do it, usually in 10 minutes or so. 0.1% NaOH or KOH in water or in 70% ethanol will do it quickly. From kgrobert <@t> rci.rutgers.edu Wed Apr 29 09:32:51 2009 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Wed Apr 29 09:19:32 2009 Subject: [Histonet] Destaining H&E slides In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835CBA@LSRIEXCH1.lsmaster.lifespan.org> References: <4EBFF65383B74D49995298C4976D1D5E03835CBA@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <49F86513.5050806@rci.rutgers.edu> Thanks to everybody for their destaining tips! -Kathleen Monfils, Paul wrote: >Anything mildly basic will quickly destain eosin. Running tap water will do it, usually in 10 minutes or so. 0.1% NaOH or KOH in water or in 70% ethanol will do it quickly. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Linda.Watson <@t> bms.com Wed Apr 29 09:39:36 2009 From: Linda.Watson <@t> bms.com (Watson, Linda) Date: Wed Apr 29 09:39:44 2009 Subject: [Histonet] Rolling sections In-Reply-To: <2A582E8156B45F468A62D1F1D20AF0837E5E6C@EX-BE08.ohsu.edu> References: <9F3B003643FC5F42930EFB83E276C8608B02EA7CE0@HNXEXCH.hendrix.local> <2A582E8156B45F468A62D1F1D20AF0837E5E6C@EX-BE08.ohsu.edu> Message-ID: <351A66CE7FB11D40AB8AC8FE5559EC7E020E4AB1F4@ushpwbmsmmp008.one.ads.bms.com> May I suggest trying the following. I too have experienced cryosectioned slices rolling up. 1) try cutting sections at -20 instead of -25 (it may be too cold) 2) try cutting sections at 6 microns instead of 8-12 microns 3) allow some frost to build up on the metal plate surface and gently with two brushes hold either side down until it gently sticks then quickly pick the specimen up with your slide. This can be very tedious and slow going but at least I was able to get the sections I needed. 4) the "breath" suggestion also worked, although not consistently, for me. 5) take your gloved "thumb" and quickly place over your sample then immediately section. This works too, but again not consistently-is worth a try. Good luck, Linda >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez >Sent: Wednesday, April 29, 2009 9:04 AM >To: Dearolf, Jennifer; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Rolling sections > >Jennifer, >It sounds like the sections are a little thick. Have you tried just >barely blowing on the tissue with your breath to warm the OCT? > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet- >bounces@lists.utsouthwestern.edu] On Behalf Of Dearolf, Jennifer >Sent: Tuesday, April 28, 2009 3:52 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Rolling sections > >Greetings, Histonetters! > >First, I wanted to thank all of you that responded to my e-mail a few >years back about freezing small pieces of muscle tissue. We have found >a method that works for us, and if anyone is interested, I would be >happy to share. It still involves the wonderfully explosive isopentane, >but it allows us to freeze fetal guinea pig muscle without artifact. > >I am writing today to ask a question about cutting frozen sections with >a cryostat. We are having problems with the sections rolling once they >come off the knife and before we can get them on a slide. We have a >Microm 505E cryostat, and we cut our OCT mounted specimens at around -25 >degrees C. We use Accuedge high profile blades, cut sections between 8 >and 12 microns thick, and use a brush to pull the sections off. But, >when we remove the brush, the sections roll up. Sometimes, they just >arc up and other times they completely roll into a jellyroll. > >I have tried putting 70% EtOH in a beaker in the cryostat. This method >was suggested to us by a vendor, but it doesn't seem to work >consistently. We can also flatten the sections with a brush, but unless >we are really quick, the sections roll up before we can get them on the >slide. It makes it difficult to get serial sections. > >Any advice would be appreciated. Thanks again for all your help so far. > >Sincerely, >Jenn > >Jennifer Dearolf, Ph.D. >Associate Professor >Biology Department >Hendrix College >1600 Washington Ave. >Conway, AR 72032 >(501) 450-4530 (office) >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Wed Apr 29 10:17:16 2009 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Apr 29 10:17:52 2009 Subject: [Histonet] CoPath computer question Message-ID: <8CB96F4B754C3B0-184-D1@FWM-D34.sysops.aol.com> We are about to start using the CoPath computer system from Sunquest. With previous systems we could obtain a surgical pathology accession number (for a frozen section for example) and put the demographic data and history in later. We have been told we cannot do that with CoPath. What do other labs with CoPath do? Thank you in advance Michael Titford Pathology USA Mobile AL From mpence <@t> grhs.net Wed Apr 29 10:41:12 2009 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Apr 29 10:41:18 2009 Subject: [Histonet] CoPath computer question In-Reply-To: <8CB96F4B754C3B0-184-D1@FWM-D34.sysops.aol.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3B25@IS-E2K3.grhs.net> We have a running list of path numbers that are kept in a book and this serves two purposes: 1. we have a number to give to cases that we are not ready to put into CoPath. We then match the case to the book when we start entering then into CoPath. and 2. it is how we continue to log cases if the computer system is down for a period of time. You may contact me off line if you have more CoPath ? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Wednesday, April 29, 2009 10:17 AM To: Histonet@pathology.swmed.edu Subject: [Histonet] CoPath computer question We are about to start using the CoPath computer system from Sunquest. With previous systems we could obtain a surgical pathology accession number (for a frozen section for example) and put the demographic data and history in later. We have been told we cannot do that with CoPath. What do other labs with CoPath do? Thank you in advance Michael Titford Pathology USA Mobile AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Wed Apr 29 11:09:06 2009 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Apr 29 11:09:12 2009 Subject: [Histonet] Rolling sections In-Reply-To: References: <200904291100263073097@foxmail.com> Message-ID: If your cryostat is set up correctly (ie, the roll plate isn't causing the rolling up), then I would try 100% EtOH instead of 70%, I have no idea why it works but sometimes, it does. Also, when our cryostat starts doing this, I just clean the blade and the knife and walk away for a few minutes--but our rolling-up is due to static electricity. You can also ground the blade by touching it with something metal. I wouldn't change your section thickness--rolling up has nothing to do with that if you've got your cryostat set right. Also don't breathe on your sections--that's not a particularly scientific way to get what you need, and you might need RNAse free sections in the future (RNAse free, your breath is not.) Emily prometheus, thief of light, giver of light, bound by the gods, must have been a book. -mark danielewski, house of leaves > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _________________________________________________ > Confidential information. Unauthorized use or disclosure prohibited. > Refer http://www.singhealth.com.sg/ContactUs/#Disclaimer > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From cbarone <@t> NEMOURS.ORG Wed Apr 29 11:37:41 2009 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Wed Apr 29 11:37:52 2009 Subject: [Histonet] Re: mid-atlantic regional histo meeting Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8033A721D@wlmmsx01.nemours.org> Histonetter's: Looking to get those needed CEU's or just want to learn something new? The states of PA, MD, NJ and DE are hosting a regional one day event on Saturday,May 16th, 2009 ...for Virginians and state and NSH members, at the Old Dominion University Higher Education Center, in Virginia Beach, VA, Topics: Seminar I: Antibody Panels, gien by tara kennedy and Seminar II: Multiplex Staining, given by Kathy Bowden (both, Application Specialists). Also a one hour open forum will be held to discuss your probleme from your labs. Both half and full day registration available. Lunch is included with full-day registration. For more Info on how to register, call: 302.651.6827. Discounts availavbe for those with State memberships and for those registering "paid in full" by May 12th. Come for the education, and stay a day for the sun and fun! Professional development is where the jobs are! From John.McGinley <@t> ColoState.EDU Wed Apr 29 14:20:27 2009 From: John.McGinley <@t> ColoState.EDU (McGinley,John) Date: Wed Apr 29 14:20:31 2009 Subject: [Histonet] Colorado Society of Histotechnology - Meeting May 8th & 9th in Colorado Springs Message-ID: Hi, The Colorado Society of Histotechnology (CSH) meeting will be held at the Cheyenne Mountain Resort in Colorado Springs, May 8th and 9th, 2009. If you are interested in attending the meeting and have not registered, please visit our website at http://www.coloradohisto.org/2009/meeting.htm. Online registration is available and payment can made on the day of the meeting (check only. Sorry, no credit cards accepted at this time). Thank you and I look forward to seeing you at the meeting. Regards, John McGinley CSH Secretary From JMitchell <@t> uwhealth.org Wed Apr 29 23:17:52 2009 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Wed Apr 29 23:17:56 2009 Subject: [Histonet] NSH Call for Awards Applications/Nominations References: <102601c7755b$887d1140$fb291c42@webhost12> Message-ID: <2108AECB05DBFF48A9C436A792155740010C349D@UWHC-MAIL03.uwhis.hosp.wisc.edu> Last Call for Nominations/Applications: You still have time to make the NSH 2009 Awards/Scholarship May 1st deadline! Each year, NSH recognizes the commitment and achievements of individuals in the histology profession, spotlights students and their achievements, and applauds the innovation of members dedicated to advancing our science. We invite you to apply and or nominate a professional for the 2009 NSH Awards and Scholarship Program. Please review the detailed descriptions of each Award and their respective criteria at www.nsh.org and then nominate the deserving professional. Be sure to submit applications by FRIDAY; the nomination deadline of May 1, 2009. Jean Mitchell - Chair, 2009 NSH Awards Committee From PIXLEYSK <@t> UCMAIL.UC.EDU Thu Apr 30 08:48:05 2009 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Thu Apr 30 08:48:17 2009 Subject: [Histonet] Rolling sections In-Reply-To: <200904291612.MJQ45438@mprelay2.uc.edu> References: <200904291612.MJQ45438@mprelay2.uc.edu> Message-ID: At one time, when we were using knives (not disposable blades), and we were using a roller plate, I found that the Shandon (now Thermo Scientific) M1 Embedding Matrix: http://www.thermo.com/com/cda/product/detail/1,1055,21902,00.html appeared to cause less rolling than OCT. You could try that. (However, now my lab is now using OCT, with disposable blades, without a roller plate, and they are not having difficulties cutting rat brains.) Another thing to check is to make sure all the settings are correct, as in previous responses: temperature, thickness, etc. Also, be sure that the knife angle is adjusted perfectly for your tissue and your temperature. Subtle changes in angle can help sectioning in many ways. Finally, if static electricity is a problem, you can try removing gloves (if you are not trying to be RNase free). Gloves can sometimes, depending on the type, increase static. Best, Sarah From rashmil_histotechnology <@t> yahoo.com Thu Apr 30 09:40:34 2009 From: rashmil_histotechnology <@t> yahoo.com (Rashmil) Date: Thu Apr 30 09:40:37 2009 Subject: [Histonet] mounting media for IHC Message-ID: <896581.68977.qm@web59307.mail.re1.yahoo.com> Hi Is there a mounting solution that is better than another for IHC slides? I am using Histomount from ZYMED. And have no problems with it. But I need to order some more and was wondering if there was one product that was better than the other? ?I was especially interested to hear about Ecomount from Biocare. Thanks, Rashmil From jtaylor <@t> meriter.com Thu Apr 30 09:47:42 2009 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Thu Apr 30 09:47:47 2009 Subject: [Histonet] MMR protein antibodies for Lynch Syndrome Message-ID: <466B666475DE6547BBB0641E540A4BB504874767D1@EXVS1.meriter.com> Hi Everyone, I'm looking for information about MLH1, MSH2, MSH6 and PMS2 and any other IVD antibody that labs are using to identify Lynch Syndrome. Would appreciate any information regarding antibody source, retrieval methods, dilutions, etc... Thank you! Jean Taylor, HT(ASCP) QIHC IHC Tech Meriter Health Services 36 S. Brooks St. Madison, WI 53715 From jmcgough <@t> clinlab.com Thu Apr 30 10:09:10 2009 From: jmcgough <@t> clinlab.com (Jason McGough) Date: Thu Apr 30 10:09:16 2009 Subject: [Histonet] Reusing Citrate Antigen Retrieval Buffer In-Reply-To: <466B666475DE6547BBB0641E540A4BB504874767D1@EXVS1.meriter.com> Message-ID: We are looking into using our .01M Citrate Antigen Retrieval Buffer in our Dako PT Link for Antigen Retrieval on our IHC stains. The question that we are wondering about is anybody reusing this solution for multiple times? If so, how many times? What is your dilution? Like the High pH Antigen Retrieval solution from Dako, it is recommended to use up to 3 time before replacement. We are having several problems with the High pH antigen retrieval solution from Dako and want to try Citrate, since that is what we have been using for many years with great results. We previously used the Pascal Pressure Cooker from Dako for Antigen Retrieval but now have the PT Link. Thank you in advance for your replies. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com From alyssa <@t> alliedsearchpartners.com Thu Apr 30 10:26:13 2009 From: alyssa <@t> alliedsearchpartners.com (Alyssa Peterson) Date: Thu Apr 30 10:26:18 2009 Subject: [Histonet] Position In Florida Message-ID: Hello, My name is Alyssa Peterson, and I am the director of lab/pathology recruitment at Allied Search Partners, and I wanted to follow up with you in reference to a full time/permanent job opportunity for a talented histotechnologist. The position is in* Auburndale, Fl area. **If this is not the job for you, however, you are interested in exploring new opportunities please send me a copy of your updated resume and what you are looking for*. Also, we offer to pay you up to $1000 for any referral that we place in a position, so please forward this to anyone who seems fit for this position and have them contact me right away! *Position: Histotechnologist* * * *Shift* *Full time, day shift* * * *Job Description:* Responsible for performance of specimen processing, embedding, cutting, routine and special histologic staining, frozen sectioning and mounting of preparations from surgical and autopsy materials for microscopic interpretation by the Pathologist. In addition, measures and describes needle core biopsies of the breast, liver, and all endoscopy biopsies. Assists Pathologist by measuring, apportioning muscle biopsies; sural nerve biopsies, and needle core biopsies of the kidney. Implements and tests new techniques and procedures. The Histotechnologist identifies tissue structures, cell components, and their staining characteristics, relating them to physiological functions, making judgments concerning the results of quality control measures. Also prepares fresh unfixed tissue for transporting to a reference lab. Requirements & Working Conditions ? High School Diploma Required. ? Completion of structured training and education in Histology. ? Licensed by the State of Florida as a Histology Technologist. ASCP certification preferred. ? Ability to stand and walk for approximately twenty percent of work time when preparing tissues, and ability to lift, carry and manipulate solvents/solvent containers, supplies, or equipment to a maximum of 25 lbs. ? Is on-call occasionally or adjusts work schedule to respond for frozen sections. ? Works in a laboratory histology section with frequent (up to thirty percent of work time) exposure to unpleasant odors, chemicals and bio-hazardous specimens. ? Potential exposure to cuts on hands and fingers from microtome knives but hazards are limited when established handling procedures are followed. -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 From tifei <@t> foxmail.com Thu Apr 30 11:24:12 2009 From: tifei <@t> foxmail.com (TF) Date: Thu Apr 30 11:24:56 2009 Subject: [Histonet] Reusing Citrate Antigen Retrieval Buffer References: Message-ID: <200905010024074693324@foxmail.com> V2UgbmV2ZXIgdGlyZWQgdGhhdCBidXQgSSB0aGluayB5b3Ugc2hvdWxkIG5vdCBkbyB0aGF0Lg0K Rm9yIHRoZSBzYWtlIG9mIHRoZSAybmQgYmF0Y2ggb2Ygc2VjdGlvbnM7IGFsc28sIHRoZSBzb2x1 dGlvbiBwSCwgY29uY2VudHJhdGlvbiwgaW9uIHN0cmVuZ3RoIGFmdGVyIGJvaWxpbmcgc2hvdWxk IGhhdmUgY2hhbmdlZC8NCg0KDQoyMDA5LTA1LTAxIA0KDQoNCg0KVEYgDQoNCg0KDQq3orz+yMuj uiBKYXNvbiBNY0dvdWdoIA0Kt6LLzcqxvOSjuiAyMDA5LTA0LTMwICAyMzoxNTozMSANCsrVvP7I y6O6IGloY3JnQGdvb2dsZWdyb3Vwcy5jb207IGhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJu LmVkdSANCrOty82juiANCtb3zOKjuiBbSGlzdG9uZXRdIFJldXNpbmcgQ2l0cmF0ZSBBbnRpZ2Vu IFJldHJpZXZhbCBCdWZmZXIgDQogDQpXZSBhcmUgbG9va2luZyBpbnRvIHVzaW5nIG91ciAuMDFN IENpdHJhdGUgQW50aWdlbiBSZXRyaWV2YWwgQnVmZmVyIGluIG91cg0KRGFrbyBQVCBMaW5rIGZv ciBBbnRpZ2VuIFJldHJpZXZhbCBvbiBvdXIgSUhDIHN0YWlucy4gVGhlIHF1ZXN0aW9uIHRoYXQg d2UNCmFyZSB3b25kZXJpbmcgYWJvdXQgaXMgYW55Ym9keSByZXVzaW5nIHRoaXMgc29sdXRpb24g Zm9yIG11bHRpcGxlIHRpbWVzPyBJZg0Kc28sIGhvdyBtYW55IHRpbWVzPyBXaGF0IGlzIHlvdXIg ZGlsdXRpb24/IExpa2UgdGhlIEhpZ2ggcEggQW50aWdlbg0KUmV0cmlldmFsIHNvbHV0aW9uIGZy b20gRGFrbywgaXQgaXMgcmVjb21tZW5kZWQgdG8gdXNlIHVwIHRvIDMgdGltZSBiZWZvcmUNCnJl cGxhY2VtZW50Lg0KV2UgYXJlIGhhdmluZyBzZXZlcmFsIHByb2JsZW1zIHdpdGggdGhlIEhpZ2gg cEggYW50aWdlbiByZXRyaWV2YWwgc29sdXRpb24NCmZyb20gRGFrbyBhbmQgd2FudCB0byB0cnkg Q2l0cmF0ZSwgc2luY2UgdGhhdCBpcyB3aGF0IHdlIGhhdmUgYmVlbiB1c2luZyBmb3INCm1hbnkg eWVhcnMgd2l0aCBncmVhdCByZXN1bHRzLiBXZSBwcmV2aW91c2x5IHVzZWQgdGhlIFBhc2NhbCBQ cmVzc3VyZSBDb29rZXINCmZyb20gRGFrbyBmb3IgQW50aWdlbiBSZXRyaWV2YWwgYnV0IG5vdyBo YXZlIHRoZSBQVCBMaW5rLiBUaGFuayB5b3UgaW4NCmFkdmFuY2UgZm9yIHlvdXIgcmVwbGllcy4N Ckphc29uIE1jR291Z2ggSFQoQVNDUCkNCkFjY291bnQgUmVwcmVzZW50YXRpdmUgLSBBbmF0b21p YyBQYXRob2xvZ3kNCkNsaW5pY2FsIExhYm9yYXRvcnkgb2YgdGhlIEJsYWNrIEhpbGxzDQoyODA1 IDV0aCBTdHJlZXQgU3VpdGUgMjEwDQpSYXBpZCBDaXR5LCBTRCA1NzcwMQ0KNjA1LTM0My0yMjY3 IEV4dCAxMjcNCjYwNS03MTgtMzc3OSAoRmF4KQ0Kam1jZ291Z2hAY2xpbmxhYi5jb20NCl9fX19f X19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fDQpIaXN0b25ldCBtYWls aW5nIGxpc3QNCkhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdQ0KaHR0cDovL2xpc3Rz LnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0DQo= From tifei <@t> foxmail.com Thu Apr 30 11:25:10 2009 From: tifei <@t> foxmail.com (=?utf-8?B?VEY=?=) Date: Thu Apr 30 11:25:39 2009 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gbW91bnRpbmcgbWVkaWEgZm9yIElIQw==?= References: <896581.68977.qm@web59307.mail.re1.yahoo.com> Message-ID: <200905010025053558870@foxmail.com> Hi, we are using DAKO fluorescent mounting medium. We also have DAPI-mouting medium so that you dont have to stain for DAPI/hoechest. 2009-05-01 TF ???? Rashmil ????? 2009-04-30 22:44:48 ???? histonet ??? ??? [Histonet] mounting media for IHC Hi Is there a mounting solution that is better than another for IHC slides? I am using Histomount from ZYMED. And have no problems with it. But I need to order some more and was wondering if there was one product that was better than the other? ? was especially interested to hear about Ecomount from Biocare. Thanks, Rashmil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From collette2 <@t> mail.llnl.gov Thu Apr 30 12:16:29 2009 From: collette2 <@t> mail.llnl.gov (Nicole Collette) Date: Thu Apr 30 12:16:45 2009 Subject: [Histonet] IHC double labeling question Message-ID: Hi, All, I am starting to do some IHC on FFPE mouse tissues, and have several antibodies working individually on my tissues (with the same retrieval protocol). The next step is to move on to double labeling, and my generic protocol calls for each label to be done sequentially (primary, secondary, followed by primary, secondary). My question is, if both of my primary antibodies are raised in different species, and are also different from my host species, can they be done together (mix the primaries for one incubation, mix the secondaries for detection)? It would save a day. I expect to see colocalization, is it better to do both primaries in one incubation so that binding of one doesn't exclude the other? I understand that I will have better control over the post-antibody washes if I do them separately, but is there another reason to do them sequentially if the retrieval is the same? Thanks for the advice! Sincerely, Nicole Collette LLNL/UCB From Kimberly.Marshall <@t> ahss.org Thu Apr 30 12:42:01 2009 From: Kimberly.Marshall <@t> ahss.org (Marshall, Kimberly) Date: Thu Apr 30 12:42:42 2009 Subject: [Histonet] Tissue release Message-ID: Hello Histo net. I have asked before for your help with this but it was a while ago so I could use some more input. How do the other Histology Labs out there handle the release of fetal tissue less than 500 grams or less than 20 weeks? We have had this issue several times in the past few years and each time the hospital and Pathologist have handled it a little differently. I need to submit a policy to the legal folks and am not sure where to start. I think I also need to create a consent form the patient should sign before being release. Any help would be appreciated. Kimberly ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From carl.hobbs <@t> kcl.ac.uk Thu Apr 30 13:17:35 2009 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Thu Apr 30 13:18:42 2009 Subject: [Histonet] RE: mounting media for IHC Message-ID: <11D9615B89C10747B1C985966A63D7CA2961FE0DB3@KCL-MAIL04.kclad.ds.kcl.ac.uk> Hi. I use DPX from VWR for all of my std dye-based stainings (such as H&E, Alcian blue/PAS) and also for my DAB IHC: 500ml for 35UK?. I note that the equivilent Zymed product, Histomount is a similar UK price.....but, for 15ml. Perhaps I am wrong? I have used Histomount and DPX and do not notice any difference to the stainings under the microscope. carl ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: 30 April 2009 18:03 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 65, Issue 51 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Colorado Society of Histotechnology - Meeting May 8th & 9th in Colorado Springs (McGinley,John) 2. NSH Call for Awards Applications/Nominations (Mitchell Jean A) 3. Rolling sections (Pixley, Sarah (pixleysk)) 4. mounting media for IHC (Rashmil) 5. MMR protein antibodies for Lynch Syndrome (Taylor, Jean) 6. Reusing Citrate Antigen Retrieval Buffer (Jason McGough) 7. Position In Florida (Alyssa Peterson) 8. Re: Reusing Citrate Antigen Retrieval Buffer (TF) 9. Re: mounting media for IHC ( TF ) ---------------------------------------------------------------------- Message: 1 Date: Wed, 29 Apr 2009 13:20:27 -0600 From: "McGinley,John" Subject: [Histonet] Colorado Society of Histotechnology - Meeting May 8th & 9th in Colorado Springs To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, The Colorado Society of Histotechnology (CSH) meeting will be held at the Cheyenne Mountain Resort in Colorado Springs, May 8th and 9th, 2009. If you are interested in attending the meeting and have not registered, please visit our website at http://www.coloradohisto.org/2009/meeting.htm. Online registration is available and payment can made on the day of the meeting (check only. Sorry, no credit cards accepted at this time). Thank you and I look forward to seeing you at the meeting. Regards, John McGinley CSH Secretary ------------------------------ Message: 2 Date: Wed, 29 Apr 2009 23:17:52 -0500 From: "Mitchell Jean A" Subject: [Histonet] NSH Call for Awards Applications/Nominations To: Message-ID: <2108AECB05DBFF48A9C436A792155740010C349D@UWHC-MAIL03.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" Last Call for Nominations/Applications: You still have time to make the NSH 2009 Awards/Scholarship May 1st deadline! Each year, NSH recognizes the commitment and achievements of individuals in the histology profession, spotlights students and their achievements, and applauds the innovation of members dedicated to advancing our science. We invite you to apply and or nominate a professional for the 2009 NSH Awards and Scholarship Program. Please review the detailed descriptions of each Award and their respective criteria at www.nsh.org and then nominate the deserving professional. Be sure to submit applications by FRIDAY; the nomination deadline of May 1, 2009. Jean Mitchell - Chair, 2009 NSH Awards Committee ------------------------------ Message: 3 Date: Thu, 30 Apr 2009 09:48:05 -0400 From: "Pixley, Sarah (pixleysk)" Subject: [Histonet] Rolling sections To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" At one time, when we were using knives (not disposable blades), and we were using a roller plate, I found that the Shandon (now Thermo Scientific) M1 Embedding Matrix: http://www.thermo.com/com/cda/product/detail/1,1055,21902,00.html appeared to cause less rolling than OCT. You could try that. (However, now my lab is now using OCT, with disposable blades, without a roller plate, and they are not having difficulties cutting rat brains.) Another thing to check is to make sure all the settings are correct, as in previous responses: temperature, thickness, etc. Also, be sure that the knife angle is adjusted perfectly for your tissue and your temperature. Subtle changes in angle can help sectioning in many ways. Finally, if static electricity is a problem, you can try removing gloves (if you are not trying to be RNase free). Gloves can sometimes, depending on the type, increase static. Best, Sarah ------------------------------ Message: 4 Date: Thu, 30 Apr 2009 07:40:34 -0700 (PDT) From: Rashmil Subject: [Histonet] mounting media for IHC To: histonet@lists.utsouthwestern.edu Message-ID: <896581.68977.qm@web59307.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Is there a mounting solution that is better than another for IHC slides? I am using Histomount from ZYMED. And have no problems with it. But I need to order some more and was wondering if there was one product that was better than the other? ?I was especially interested to hear about Ecomount from Biocare. Thanks, Rashmil ------------------------------ Message: 5 Date: Thu, 30 Apr 2009 09:47:42 -0500 From: "Taylor, Jean" Subject: [Histonet] MMR protein antibodies for Lynch Syndrome To: "'ihcrg@googlegroups.com'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <466B666475DE6547BBB0641E540A4BB504874767D1@EXVS1.meriter.com> Content-Type: text/plain; charset="us-ascii" Hi Everyone, I'm looking for information about MLH1, MSH2, MSH6 and PMS2 and any other IVD antibody that labs are using to identify Lynch Syndrome. Would appreciate any information regarding antibody source, retrieval methods, dilutions, etc... Thank you! Jean Taylor, HT(ASCP) QIHC IHC Tech Meriter Health Services 36 S. Brooks St. Madison, WI 53715 ------------------------------ Message: 6 Date: Thu, 30 Apr 2009 09:09:10 -0600 From: "Jason McGough" Subject: [Histonet] Reusing Citrate Antigen Retrieval Buffer To: , Message-ID: Content-Type: text/plain; charset="us-ascii" We are looking into using our .01M Citrate Antigen Retrieval Buffer in our Dako PT Link for Antigen Retrieval on our IHC stains. The question that we are wondering about is anybody reusing this solution for multiple times? If so, how many times? What is your dilution? Like the High pH Antigen Retrieval solution from Dako, it is recommended to use up to 3 time before replacement. We are having several problems with the High pH antigen retrieval solution from Dako and want to try Citrate, since that is what we have been using for many years with great results. We previously used the Pascal Pressure Cooker from Dako for Antigen Retrieval but now have the PT Link. Thank you in advance for your replies. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com ------------------------------ Message: 7 Date: Thu, 30 Apr 2009 11:26:13 -0400 From: Alyssa Peterson Subject: [Histonet] Position In Florida To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello, My name is Alyssa Peterson, and I am the director of lab/pathology recruitment at Allied Search Partners, and I wanted to follow up with you in reference to a full time/permanent job opportunity for a talented histotechnologist. The position is in* Auburndale, Fl area. **If this is not the job for you, however, you are interested in exploring new opportunities please send me a copy of your updated resume and what you are looking for*. Also, we offer to pay you up to $1000 for any referral that we place in a position, so please forward this to anyone who seems fit for this position and have them contact me right away! *Position: Histotechnologist* * * *Shift* *Full time, day shift* * * *Job Description:* Responsible for performance of specimen processing, embedding, cutting, routine and special histologic staining, frozen sectioning and mounting of preparations from surgical and autopsy materials for microscopic interpretation by the Pathologist. In addition, measures and describes needle core biopsies of the breast, liver, and all endoscopy biopsies. Assists Pathologist by measuring, apportioning muscle biopsies; sural nerve biopsies, and needle core biopsies of the kidney. Implements and tests new techniques and procedures. The Histotechnologist identifies tissue structures, cell components, and their staining characteristics, relating them to physiological functions, making judgments concerning the results of quality control measures. Also prepares fresh unfixed tissue for transporting to a reference lab. Requirements & Working Conditions ? High School Diploma Required. ? Completion of structured training and education in Histology. ? Licensed by the State of Florida as a Histology Technologist. ASCP certification preferred. ? Ability to stand and walk for approximately twenty percent of work time when preparing tissues, and ability to lift, carry and manipulate solvents/solvent containers, supplies, or equipment to a maximum of 25 lbs. ? Is on-call occasionally or adjusts work schedule to respond for frozen sections. ? Works in a laboratory histology section with frequent (up to thirty percent of work time) exposure to unpleasant odors, chemicals and bio-hazardous specimens. ? Potential exposure to cuts on hands and fingers from microtome knives but hazards are limited when established handling procedures are followed. -- Alyssa Peterson Allied Search Partners O: 770.621.2639 ext. 4 F: 770.621.2640 ------------------------------ Message: 8 Date: Fri, 1 May 2009 00:24:12 +0800 From: "TF" Subject: Re: [Histonet] Reusing Citrate Antigen Retrieval Buffer To: "Jason McGough" , "ihcrg@googlegroups.com" , "histonet@lists.utsouthwestern.edu" Message-ID: <200905010024074693324@foxmail.com> Content-Type: text/plain; charset="gb2312" We never tired that but I think you should not do that. For the sake of the 2nd batch of sections; also, the solution pH, concentration, ion strength after boiling should have changed/ 2009-05-01 TF ???????? Jason McGough ?????????? 2009-04-30 23:15:31 ???????? ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu ?????? ?????? [Histonet] Reusing Citrate Antigen Retrieval Buffer We are looking into using our .01M Citrate Antigen Retrieval Buffer in our Dako PT Link for Antigen Retrieval on our IHC stains. The question that we are wondering about is anybody reusing this solution for multiple times? If so, how many times? What is your dilution? Like the High pH Antigen Retrieval solution from Dako, it is recommended to use up to 3 time before replacement. We are having several problems with the High pH antigen retrieval solution from Dako and want to try Citrate, since that is what we have been using for many years with great results. We previously used the Pascal Pressure Cooker from Dako for Antigen Retrieval but now have the PT Link. Thank you in advance for your replies. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 1 May 2009 00:25:10 +0800 From: " TF " Subject: Re: [Histonet] mounting media for IHC To: " rashmil_histotechnology " , " histonet " Message-ID: <200905010025053558870@foxmail.com> Content-Type: text/plain; charset="utf-8" Hi, we are using DAKO fluorescent mounting medium. We also have DAPI-mouting medium so that you dont have to stain for DAPI/hoechest. 2009-05-01 TF ???????????? Rashmil ??????????????? 2009-04-30 22:44:48 ???????????? histonet ????????? ????????? [Histonet] mounting media for IHC Hi Is there a mounting solution that is better than another for IHC slides? I am using Histomount from ZYMED. And have no problems with it. But I need to order some more and was wondering if there was one product that was better than the other? ??? was especially interested to hear about Ecomount from Biocare. Thanks, Rashmil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 65, Issue 51 **************************************** From zerfasp <@t> ors.od.nih.gov Thu Apr 30 14:24:19 2009 From: zerfasp <@t> ors.od.nih.gov (Zerfas, Patricia (NIH/OD/ORS) [E]) Date: Thu Apr 30 14:24:24 2009 Subject: [Histonet] HAM 56 Message-ID: <424D930E0319E0469DD9C61502A50E300210222D@NIHCESMLBX4.nih.gov> Does any know of a source for the antibody HAM 56? DAKO is no longer manufacturing it. I tried Biocare and an internet search with no luck. Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 From adam_anthony <@t> mba.berkeley.edu Thu Apr 30 14:24:25 2009 From: adam_anthony <@t> mba.berkeley.edu (Adam Anthony) Date: Thu Apr 30 14:24:30 2009 Subject: [Histonet] UC Berkeley students in need of help: follow-up Message-ID: <671FB8AFBC3FD548A2CA262D71937E6A01596DB779CE@EXMAIL7.haas.uc.berkeley.edu> Histoneters, Thank you for your support of our class project at UC Berkeley. At the time of writing, 35 individuals have responded to the survey, which was posted just 36 hours ago! As a reminder, we are 4 MBA students conducting market research through a simple online survey. Our focus is on ways in which labs can cut significant costs on reagents and materials, which I'm sure that many of you are tasked with doing. Due to the unanticipated response, we've decided to leave the survey open through the weekend (and possible a few days longer). If you would like to participate in this quick 11 question survey, here's how you can: Link to online survey- should only take 2 minutes to complete: http://www.surveymonkey.com/s.aspx?sm=X3ssptgNq4oh7o0vwy1gCg_3d_3d Please contact me with any questions. Please note that results will be made available to respondents upon request. Thanks, Adam Anthony adam_anthony@mba.berkeley.edu From portera <@t> msu.edu Thu Apr 30 14:45:30 2009 From: portera <@t> msu.edu (Amy Porter) Date: Thu Apr 30 14:45:38 2009 Subject: [Histonet] IHC double labeling question References: Message-ID: <490480201B644954962DEDB400F46A3B@histolab> I would do them together making sure your end volume holds the same concentration of each reagent as you use singely. It should work fine for you and cut down on your time. You will need to find a generic protein block to utilize if your secondaries are made in two different hosts. If your antigens are co-localized you may possibly have a difficult time demonstrating them together. Good luck, Amy Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Nicole Collette" To: Sent: Thursday, April 30, 2009 1:16 PM Subject: [Histonet] IHC double labeling question > Hi, All, > > I am starting to do some IHC on FFPE mouse tissues, and have several > antibodies working individually on my tissues (with the same retrieval > protocol). The next step is to move on to double labeling, and my generic > protocol calls for each label to be done sequentially (primary, secondary, > followed by primary, secondary). > > My question is, if both of my primary antibodies are raised in different > species, and are also different from my host species, can they be done > together (mix the primaries for one incubation, mix the secondaries for > detection)? It would save a day. I expect to see colocalization, is it > better to do both primaries in one incubation so that binding of one > doesn't exclude the other? I understand that I will have better control > over the post-antibody washes if I do them separately, but is there > another reason to do them sequentially if the retrieval is the same? > Thanks for the advice! > > Sincerely, > Nicole Collette > LLNL/UCB > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From david.kinsley <@t> spcorp.com Thu Apr 30 15:56:08 2009 From: david.kinsley <@t> spcorp.com (Kinsley, David) Date: Thu Apr 30 15:56:14 2009 Subject: [Histonet] Oil red o mounting media Message-ID: Hi, I will be staining many samples for Oil red O in the near future. I was wondering if there were any aqueous mounting medias out there that perform superior to Glycergel? Thanks Dave ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From DKnutson <@t> primecare.org Thu Apr 30 16:03:26 2009 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Thu Apr 30 16:03:22 2009 Subject: [Histonet] Controls slides Message-ID: <4F0B7161A6CD524FAD8017D52E1553400A76891C@exchangent> I just wanted to thank all you fellow histonetters who answered my plea for H. Pylori and / or GMS control blocks. They are great! This trading of controls is so much nicer than having to buy them. Thanks again for your help! Deanne Knutson From vapatpxs <@t> yahoo.com Thu Apr 30 17:22:08 2009 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Thu Apr 30 17:28:52 2009 Subject: [Histonet] Processing Fat for Paraffin Message-ID: <490013.63581.qm@web46103.mail.sp1.yahoo.com> Hello out there in fat processing land, I have been given human fat samples and need to embed them in paraffin. In the past I've used a VIP processor for this and now I have an Autotechnicon (vintage dual model) with a timing wheel. I know I need to process these fatties slowly, my question is--can I use 2 hours per step and have it turn out OK? I have a timing wheel punched out for 2 hour steps. My steps would be alcohols: 70, 80, 95 x 2, 100 x 3, Citrisolv x3, paraffin x2 and another paraffin step under vacuum. Let me know your wise and experienced opinions or protocols. I don't have anything else to use except the 43 year old Autotechnicon so don't even suggest it. You make me feel bad that I can't get my research foundation to buy me something new or even newer. ;-) Stewing in somebody else's fat (eew!), Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 From laurie.reilly <@t> jcu.edu.au Thu Apr 30 18:15:16 2009 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Thu Apr 30 18:17:43 2009 Subject: [Histonet] Processing Fat for Paraffin In-Reply-To: <490013.63581.qm@web46103.mail.sp1.yahoo.com> References: <490013.63581.qm@web46103.mail.sp1.yahoo.com> Message-ID: <51365B7576FD4AF89A5651BBA32BC36B@health.ad.jcu.edu.au> Dear Paula, Don't knock the Autotechnicon, they can still do a great job of processing. We processed with a Shandon-Elliott Duplex up until 3 years ago and it was great (apart from the fumes in the lab). We run the cycle below as a 16hour, overnight process successfully. I don't know if your citrisolv will be as effective at dissolving fat as xylene, but it would be easy to do a little trial. The major problem with processing fatty tissues, assuming that they are fixed properly, is that Ethanol is not a good solvent for fat and therefore connot penetrate the tissues completely, so the tissues are inadequately dehydrated. We have had some success with lipomas by adding a "degreasing" step of xylene into the processing schedule. 70% ethanol 80% ethanol 90% ethanol 95% ethanol Absolute ethanol Xylene Absolute ethanol Xylene Xylene Paraffin Paraffin Paraffin The first Absolute ethanol will dehydrate the tissue to some extent. The next Xylene step will remove most of the fat and then the second Absolute ethanol can complete the dehydration. A compromise situation that we use routinely is to have Absolute ethanol 50:50 Absolute ethanol:Xylene Xylene This is not quite as effective but it is less disruptive to the normal schedule and handles moderately fatty tissues. Good luck with your fat. Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Va Paula Sicurello Sent: Friday, 1 May 2009 8:22 AM To: HistoNet; MSA BB Subject: [Histonet] Processing Fat for Paraffin Hello out there in fat processing land, I have been given human fat samples and need to embed them in paraffin. In the past I've used a VIP processor for this and now I have an Autotechnicon (vintage dual model) with a timing wheel. I know I need to process these fatties slowly, my question is--can I use 2 hours per step and have it turn out OK? I have a timing wheel punched out for 2 hour steps. My steps would be alcohols: 70, 80, 95 x 2, 100 x 3, Citrisolv x3, paraffin x2 and another paraffin step under vacuum. Let me know your wise and experienced opinions or protocols. I don't have anything else to use except the 43 year old Autotechnicon so don't even suggest it. You make me feel bad that I can't get my research foundation to buy me something new or even newer. ;-) Stewing in somebody else's fat (eew!), Paula :-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lmoffatt <@t> susquehannahealth.org Tue Apr 28 13:06:04 2009 From: lmoffatt <@t> susquehannahealth.org (Moffatt, Loretta) Date: Fri May 1 12:18:20 2009 Subject: [Histonet] xylene Message-ID: We have recently had a problem with poor tissue processing (soft to cut/ poor staining). None of the tissue was processed the first time, and ultimately, some fragile tissue did not survive. The alcohols checked out and the processor, itself, does not appear to be an issue. We used recycled xylene in the processor first, followed by fresh xylene. The xylene, which came from a CBG recycler, failed the test for contamination, after the fact. Has anyone seen this kind of problem? We would appreciate any input. Thank you. Loretta Moffatt, MT(ASCP),MHA Laboratory Manager 777 Rural Avenue Williamsport, PA 17701 570-321-2326 (F)570-321-2489 lmoffatt@susquehannahealth.org Patience is never more important than when you are on the verge of losing it. Confidentiality Notice: This message and any attachments originate by electronic mail from Susquehanna Health System and their subsidiaries/affiliates ("SHS"). Both this document and any attachments are intended for the sole use of the addressee indicated above and may contain proprietary, privileged and/or confidential information. If you are not the intended recipient of this message, you are hereby notified that any use or disclosure of this information is strictly prohibited. If you received this message in error, or have reason to believe you are not authorized to receive it, please notify the sender by reply email, with a copy to ITSecurity@susquehannahealth.org < mailto:ITSecurity@susquehannahealth.org>, and then promptly delete the original and reply messages. Thank you for your cooperation. From Chuck.Rippin <@t> dako.com Tue Apr 28 15:58:02 2009 From: Chuck.Rippin <@t> dako.com (Chuck Rippin) Date: Fri May 1 12:18:24 2009 Subject: [Histonet] Need refurbished H&E stainer Message-ID: <8B07D141BCDE434285DC12B3290E3FB30310CDD1@exbackca.caus.dako.net> Sally; I am the rep from Dako. Would you please provide your contact information? Thanks Chuck Rippin From JeanetteMontgomery <@t> shannonhealth.org Wed Apr 29 10:09:30 2009 From: JeanetteMontgomery <@t> shannonhealth.org (Jeanette Montgomery) Date: Fri May 1 12:18:27 2009 Subject: [Histonet] Texas Society for Histotechnology 2009 Message-ID: <45C264396E872B49B6D818E3CD18E38A097E16EA30@SVR-EXCHANGECCR.shannonhealth.local> Hello Was interested in receiving cost information on the meeting in Austin. Would be driving down on Saturday morning, for Saturday attendance only. Possibly to attend one or two sessions depending on start time. Am not a member of Texas society. Am registered HTL/CT ASCP. Please send appropriate schedules, forms, and information. Thanks Jeanette Montgomery Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please destroy all copies of the original message. From sylviad67 <@t> live.com Wed Apr 29 16:24:51 2009 From: sylviad67 <@t> live.com (Sylvia Don) Date: Fri May 1 12:18:31 2009 Subject: [Histonet] Cancer Diagnostics v. Surgipath Message-ID: Does anyone know what happened between Cancer Diagnostics and Surgipath? I heard that someone who worked with Surgipath secretly and possibly illegally set up Cancer Diagnostics and was funneling sales leads away from Surgipath to Cancer Diagnostics. I purchase from CDI and I am worried that my products will be interrupted, but I cannot get any answers. I am sorry if I am feeding the rumor mill, but I am just worried. Sylvia D. _________________________________________________________________ Windows Live? Hotmail?:?more than just e-mail. http://windowslive.com/online/hotmail?ocid=TXT_TAGLM_WL_HM_more_042009