From carl.hobbs <@t> kcl.ac.uk Mon Sep 1 14:05:35 2008 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Mon Sep 1 14:06:35 2008 Subject: [Histonet] RE: DAB: old-fashioned style Message-ID: <11D9615B89C10747B1C985966A63D7CA2857ECB49F@KCL-MAIL04.kclad.ds.kcl.ac.uk> I do it in the Old-fashioned way too;-) May not be as sexy but, works every time and the costs are small, relative to commercial kits...;-) I jest prefer to make a bulk and freeze the aliquots, rather than weigh out DAB! So: 5g of Tim's most excellent "dust"...aka DAB. Place 50 1-5ml m'centrifuge tubes in a rack. Heat 50ml distilled water in a 250ml beaker to ~30C Add enough heated water to the 5g bottle to 3/4 fill it, screw on the lid, mix gently, in a fume cabinet. Return this to the 250ml beaker. No more than a couple of backwards and forwards between bottle and beaker will ensure complete dissolution of stock DAB.( yep, if you wish , add a magnetic stirrer bar) Aliquot 1ml into each, in a rack. Place rack in freezer for a couple of hrs while you have lunch......store in a box at -20C I like to incubate my slides in a 200ml container of DAB ( I do not usually like to apply it sequentially onto slides on a tray....such a pain but, but there are always exceptions. So you can make up smaller aliquots, if you wish! Or just use 200mls...the cost is trivial). I defrost a 1ml aliquot of DAB in running hot water, make up my 200ml TB buffer ( from a 10x stock), add 200microL 100vol H2O2, my 1ml of defrosted DAB stock, mix well and add my rack of slides, gently up-and-down the rack a couple of times and then leave for 10 mins. Then take out rack into tap water and give it a good rinse in running water before going into Haemalum.....etc Carl PS: do not leave the DAB aliquot in hot water for longer than it takes to dissolve/mix all solutions well. Also, reaction of H2O2/HRP/DAB should go to completion within 5mins but....after 10mins, imho, using above relative volumes, the effective reaction is complete, for my IHC reactions. Sure, I am very happy to state my ways, if only to test them within Histonet! Constructive critiscisms would be very welcome. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: 01 September 2008 18:03 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 58, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: (para)formaldehyde (Tony Henwood) 2. RE: (para)formaldehyde (Tony Henwood) 3. RE: DAB: old-fashioned style! (Tony Henwood) ---------------------------------------------------------------------- Message: 1 Date: Mon, 1 Sep 2008 09:26:51 +1000 From: "Tony Henwood" Subject: RE: [Histonet] (para)formaldehyde To: "MKing" , Message-ID: Content-Type: text/plain; charset="us-ascii" I agree Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MKing Sent: Saturday, 30 August 2008 12:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (para)formaldehyde The statement that 4% paraformaldehyde solution contains nothing but water and formaldehyde is simply not true: as has been discussed thoroughly and often on Histonet (see archives), formaldehyde in aqueous solution spontaneously decomposes. This is precisely why formalin solutions are used, with methanol 'parking' the chemical reaction. Without measurement one never knows exactly how much formaldehyde is really present in solutions made from paraformaldehyde, although freshly made solutions will have concentrations closest to 4%. It is highly improbable that 3.8 and 4.0% solutions would produce any detectable and reproducible differences on any histological procedure, although the methanol added to formalin might. Mike King UF Pharmacology & Therapeutics ----------------- Message: 5 Date: Wed, 27 Aug 2008 17:26:19 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] (para)formaldehyde To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C54@LSRIEXCH1.lsmaster.lifespan.or g> Content-Type: text/plain; charset="iso-8859-1" 10% formalin and 4% paraformaldehyde are interchangeable for most purposes (in histology at least). However there are a couple of minor differences. First, commercial formaldehyde solution contains 37% to 38% formaldehyde. Therefore diluting it 1:9 results in a solution containing 3.7% to 3.8% formaldehyde, while a 4% solution of paraformaldehyde in water contains a full 4% formaldehyde. Secondly, commercial formaldehyde solution contains 10% to 15% methanol as a preservative. Therefore diluting it 1:9 results in a solution containing 1.0% to 1.5% methanol. This is not a problem for most histological applications, but it could be a problem in a procedure where sources of methylation have to be avoided. 4% paraformaldehyde solution contains no methanol - nothing but water and formaldehyde. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 2 Date: Mon, 1 Sep 2008 09:32:17 +1000 From: "Tony Henwood" Subject: RE: [Histonet] (para)formaldehyde To: , , "MKing" Message-ID: Content-Type: text/plain; charset="iso-8859-1" AND I agree. Its now getting more precise in the Science. I would suggest that the NSH put together a committee to determine policy on the nomenclature of formalin solutions so that we can call the lemon the lemon and not the "Sprite" or "Solo" that we seem to have found ourselves in. Hopefully we can then agree on what we are going to call the stuff. There is definitely enough publications out there to start from. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, 30 August 2008 12:28 AM To: histonet@lists.utsouthwestern.edu; MKing Subject: Re: [Histonet] (para)formaldehyde Just for detail sake, methanal (formaldehyde) does NOT decompose in water, it just reacts with it to become methanediol (methylene glycol) and the amount not reacting and remaining as formaldehyde has been estimated in about 0.1% of the total in a 4% formalin solution. The alcohol methanol is added NOT to prevent the formaldehyde hydration, but to as a stabilizer, to retard its polymerization, so it is very likely that between the moment a 4% formaldehyde solution is prepared using paraformaldehyde to the moment it starts to penetrate, bind and cross-link it will be very close to 4% Ren? J. --- On Fri, 8/29/08, MKing wrote: From: MKing Subject: [Histonet] (para)formaldehyde To: histonet@lists.utsouthwestern.edu Date: Friday, August 29, 2008, 10:14 AM The statement that 4% paraformaldehyde solution contains nothing but water and formaldehyde is simply not true: as has been discussed thoroughly and often on Histonet (see archives), formaldehyde in aqueous solution spontaneously decomposes. This is precisely why formalin solutions are used, with methanol 'parking' the chemical reaction. Without measurement one never knows exactly how much formaldehyde is really present in solutions made from paraformaldehyde, although freshly made solutions will have concentrations closest to 4%. It is highly improbable that 3.8 and 4.0% solutions would produce any detectable and reproducible differences on any histological procedure, although the methanol added to formalin might. Mike King UF Pharmacology & Therapeutics ----------------- Message: 5 Date: Wed, 27 Aug 2008 17:26:19 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] (para)formaldehyde To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C54@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" 10% formalin and 4% paraformaldehyde are interchangeable for most purposes (in histology at least). However there are a couple of minor differences. First, commercial formaldehyde solution contains 37% to 38% formaldehyde. Therefore diluting it 1:9 results in a solution containing 3.7% to 3.8% formaldehyde, while a 4% solution of paraformaldehyde in water contains a full 4% formaldehyde. Secondly, commercial formaldehyde solution contains 10% to 15% methanol as a preservative. Therefore diluting it 1:9 results in a solution containing 1.0% to 1.5% methanol. This is not a problem for most histological applications, but it could be a problem in a procedure where sources of methylation have to be avoided. 4% paraformaldehyde solution contains no methanol - nothing but water and formaldehyde. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 3 Date: Mon, 1 Sep 2008 09:35:08 +1000 From: "Tony Henwood" Subject: RE: [Histonet] DAB: old-fashioned style! To: "Kim Merriam" , "Histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-7" Try this DAB Reagent: 1. Take 40ml Tris Buffer-Wash and add 6mg of DAB (3,3, Diaminobenzidine Tetrahydrochloride Grade II (Sigma D5637)), mix. 2. Add 50?l of hydrogen peroxide, mix. 3. The solution is ready to use. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Saturday, 30 August 2008 3:07 AM To: Histonet Subject: [Histonet] DAB: old-fashioned style! Hi All, It's been many moons since I had to make DAB by hand and I have lost my recipe. Can anyone let me know how to make DAB from powder and H2O2? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 58, Issue 1 *************************************** From Allison_Scott <@t> hchd.tmc.edu Mon Sep 1 17:41:39 2008 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Mon Sep 1 17:41:51 2008 Subject: [Histonet] Job Description for cytology assistant Message-ID: <1872B4A455B7974391609AD8034C79FC082E52@LBEXCH01.hchd.local> Hi histonetters, Can someone be so kind as to share a job description with me for a cytology dept. assistant. They would be required to process gyns, non gyns and FNA's. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From jayncube <@t> yahoo.com Tue Sep 2 03:08:44 2008 From: jayncube <@t> yahoo.com (j ncube) Date: Tue Sep 2 03:08:49 2008 Subject: [Histonet] processing fibroadenomas Message-ID: <303863.50105.qm@web63404.mail.re1.yahoo.com> Hie, ? May someone out there please help me with a protocol for processing fibroadenomas and other dense tissues. Jabulani Ncube Biomedical Scientist Department of Pathology Diagnofirm Medical Laboratories Tel:+(267)3950007 Mobile:+(267)72 917 869 From b-frederick <@t> northwestern.edu Tue Sep 2 08:11:18 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Sep 2 08:11:37 2008 Subject: [Histonet] Gustav Message-ID: <002a01c90cfd$6749f890$d00f7ca5@lurie.northwestern.edu> Hope everyone down on the gulf coast that was affected by Gustav are doing okay! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 From michelle.steinkrauss <@t> novartis.com Tue Sep 2 12:36:24 2008 From: michelle.steinkrauss <@t> novartis.com (michelle.steinkrauss@novartis.com) Date: Tue Sep 2 12:36:36 2008 Subject: [Histonet] Michelle Steinkrauss is out of the office. Message-ID: I will be out of the office starting 09/02/2008 and will not return until 09/03/2008. If you require immediate assistance for study-related matters, please contact Michelle Broome at x 47477. For clinical pathology assistance, please contact Rhonda Osborne at x 47099. From cmiller <@t> physlab.com Tue Sep 2 14:05:48 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Sep 2 14:05:57 2008 Subject: [Histonet] Documenting control tissue source? In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5AD@lmhsmail.lmhealth.org> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5AD@lmhsmail.lmhealth.org> Message-ID: <00a101c90d2e$e95fdbc0$3d02a8c0@plab.local> I agree with you. We just had our inspection in July and it wasn't an issue. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, August 15, 2008 6:12 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Documenting control tissue source? We are finishing up our JCAHO inspection today. The inspector asked about our control tissues and where we documented the source. We get nearly all of our controls from patient samples and she said that most histo labs mark their control blocks with the source case number. They then keep records of the control source used when staining new cases. I can see no logical reason to keep this info. You use a control appropriate for the element being stained. Why does it matter where the control came from? Am I wrong? Do you do this? Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From trathborne <@t> somerset-healthcare.com Tue Sep 2 14:26:52 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Sep 2 14:27:00 2008 Subject: [Histonet] Job opening Message-ID: Somerset Medical Center, located in central NJ, has a daytime opening for a FT Histotech. Embedding, microtomy, knowledge of special stains and IHC required. Apply online at http://somersetmedicalcenter.com/, or send resume to: trathborne@somerset-healthcare.com Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville,NJ 08876 908-595-2367 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From sbruce <@t> vetpathservicesinc.com Tue Sep 2 15:51:40 2008 From: sbruce <@t> vetpathservicesinc.com (Suzanne Bruce) Date: Tue Sep 2 15:51:48 2008 Subject: [Histonet] NSH Conference Message-ID: <26DB1FDFBF9EE14AB3AACC873519A4A2515149@vpss1.VetPathServicesInc.local> Hello, this message is mainly for the boneheads and plastics users (specifically Technovit 7200, GMA and others using the Exakt system). I will be attending the NSH conference for the first time. I don't know many people involved in plastics, so I was wondering who else would be attending that is familiar w/the plastics I mentioned above. I'd certainly like to meet a few of you! Also any friendly words of advice about getting the most out of the conference would be appreciated! Thanks so much! ________________________________________ Suzanne Bruce, R.V.T. Histologist / Necropsy Coordinator Vet Path Services, Inc. (VPS) 6450 Castle Dr. Mason, OH 45040 Lab: (513) 469-0777 ext 19 Fax: (513) 469-2474 Email: sbruce@vetpathservicesinc.com www.vetpathservicesinc.com From jstaruk <@t> masshistology.com Tue Sep 2 19:26:31 2008 From: jstaruk <@t> masshistology.com (jstaruk) Date: Tue Sep 2 19:26:36 2008 Subject: [Histonet] Differentiating a neutrophil from a de-granulated eosinophil ....antibody or stain? In-Reply-To: <26DB1FDFBF9EE14AB3AACC873519A4A2515149@vpss1.VetPathServicesInc.local> Message-ID: Hi all, Does anyone know if the anti-eosinophil antibody will adhere to a degranulated eosinophil or does the antibody only stick to the eosinophil granules and not the cell itself? I'm performing a differential count in a certain fluid and I'm highly suspicious that many of the neutrophils are actually de-granulated eosinophils (a bit larger, thicker, less segmented nuclei, etc). Is there any other method to differentiate a neutrophils from a degranulated eosinophil that I'm not aware of? Thank you! Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com From marktarango <@t> gmail.com Tue Sep 2 19:32:14 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Sep 2 19:32:21 2008 Subject: [Histonet] Differentiating a neutrophil from a de-granulated eosinophil ....antibody or stain? In-Reply-To: References: <26DB1FDFBF9EE14AB3AACC873519A4A2515149@vpss1.VetPathServicesInc.local> Message-ID: <5b6eb13e0809021732j11d1c969nc41921d7f3628091@mail.gmail.com> Got a picture? On 9/2/08, jstaruk wrote: > Hi all, > > Does anyone know if the anti-eosinophil antibody will adhere to a > degranulated eosinophil or does the antibody only stick to the eosinophil > granules and not the cell itself? > > I'm performing a differential count in a certain fluid and I'm highly > suspicious that many of the neutrophils are actually de-granulated > eosinophils (a bit larger, thicker, less segmented nuclei, etc). Is there > any other method to differentiate a neutrophils from a degranulated > eosinophil that I'm not aware of? > > Thank you! > > Jim > > _____________________ > Jim Staruk > Mass Histology Service > www.masshistology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amosbrooks <@t> gmail.com Wed Sep 3 09:24:47 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Sep 3 09:24:55 2008 Subject: [Histonet] BUGZ Message-ID: <582736990809030724p3a842a4fscedd9fc487f35c21@mail.gmail.com> Hi, Here's an oddball question. I have a researcher that wants to bring me mosquitoes for paraffin sectioning. I immediatly had a chill when thinking of cutting the chitin. Has anyone worked with similar bugs, and if so how did you process them to make them cuttable? What fixative would be best? Thanks, Amos From Lkorczyn <@t> gbmc.org Wed Sep 3 09:26:11 2008 From: Lkorczyn <@t> gbmc.org (LAURA KORCZYNSKI) Date: Wed Sep 3 09:26:34 2008 Subject: [Histonet] Histology Position Available - Baltimore Maryland Message-ID: <48BE6640.B3F2.00C2.0@gbmc.org> HISTOTECHNOLOGIST Full Time -Day Shift/1 Saturday Every 6 weeks Fully automated histology team is looking for an experienced individual that is ASCP registered or is eligible. At least 2 years histology experience is desired. Duties include embedding, cutting, H&E and Histochemical Staining of tissue specimens. Free on-site parking. Qualified candidates can apply on-line to GBMC.org and/or send resume to LKorczyn@gbmc.org. Laura Korczynski Histology Supervisor Greater Baltimore Medical Center Phone: 443-849-2831 Fax: 443-849-3016 _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. From shive003 <@t> umn.edu Wed Sep 3 09:49:20 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Sep 3 09:49:30 2008 Subject: [Histonet] IHC buffer expiration date Message-ID: <3FEAA312E14E47989C362072225F5E04@auxs.umn.edu> Hi folks, What is the general concensus on expiration dates of IHC buffers that one prepares from scratch and keeps at room temperature (TBS, Citrate, etc.)? I've found one source that states 3 months as a storage time period. Is this the general assumption or do you use a different timeline? Jan Shivers Histology/IHC/EM University of Minnesota From lac65 <@t> email.med.yale.edu Wed Sep 3 10:09:48 2008 From: lac65 <@t> email.med.yale.edu (Lori Charette) Date: Wed Sep 3 10:08:03 2008 Subject: [Histonet] Hello Message-ID: <48BEA8BC.1070508@email.med.yale.edu> Hello -- Lori A. Charette, MSM, MT, HT (ASCP) Manager, Research Histology / Tissue Microarray Facility 310 Cedar St. LH-202 New Haven, CT 06510 phone (203)737-4198 fax (203) 737-1198 From pjfnefro <@t> duke.edu Wed Sep 3 10:33:11 2008 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Wed Sep 3 10:33:19 2008 Subject: [Histonet] Hello In-Reply-To: <48BEA8BC.1070508@email.med.yale.edu> References: <48BEA8BC.1070508@email.med.yale.edu> Message-ID: <6EEF0B68-5BD2-482E-A13B-87C71F995155@duke.edu> Well, hello right back, Lori! Nice to hear from you, but did you have a question or suggestion for the group? :-) -Pat Flannery (Duke wise guy) On Sep 3, 2008, at 11:09 AM, Lori Charette wrote: > Hello > > -- > Lori A. Charette, MSM, MT, HT (ASCP) > Manager, Research Histology / Tissue Microarray Facility > 310 Cedar St. > LH-202 > New Haven, CT 06510 > phone (203)737-4198 > fax (203) 737-1198 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NHeath <@t> Lifespan.org Wed Sep 3 10:59:59 2008 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Wed Sep 3 11:00:04 2008 Subject: [Histonet] What to do when there is no liquid nitrogen :p Message-ID: <130E8991F210424096EFC6F42EA33B240337B930@LSCOEXCH1.lsmaster.lifespan.org> Hi Everyone, Does anyone have alternate methods for snap freezing muscle when there is no liquid nitrogen?? I've been told to make a slurry with isopentane & dry ice or to put isopentane in a plastic cup (?) and put into the minus 70* freezer till it gets cold. Also how volatile / dangerous is isopentane to work with??? Other than a flammable cabinet are there any other things I need to know about isopentane??? Thanks, Nancy From CarterK <@t> MedImmune.com Wed Sep 3 12:06:44 2008 From: CarterK <@t> MedImmune.com (Carter, Kendra) Date: Wed Sep 3 12:07:23 2008 Subject: [Histonet] Career Opportunities Message-ID: <4FF56D2ABFD3FE47A0C41D5631372133F4AD5C@MD1EV001.medimmune.com> Good Afternoon Everyone, A close friend of mine is looking to get into the scientific field after graduation. Does anyone know of an entry level position in the Maryland, DC, and Northern Virginia area? Kendra Leigh Carter GLP Documentation & Archive Specialist MedImmune One MedImmune Way Gaithersburg, MD 20878 PH: 301-398-4956 Fax: 301-398-9956 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From rosenfeldtek <@t> hotmail.com Wed Sep 3 12:08:07 2008 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Wed Sep 3 12:08:12 2008 Subject: [Histonet] What to do when there is no liquid nitrogen :p In-Reply-To: <130E8991F210424096EFC6F42EA33B240337B930@LSCOEXCH1.lsmaster.lifespan.org> References: <130E8991F210424096EFC6F42EA33B240337B930@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: Yes, you can use isopentane-dry ice slurry, or dry ice and ethanol. Yes, Isopentane is very volatile and very, very flammable. No, for heaven's sake don't put it in your -80 freezer. The dry ice will chill it down just fine. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Wed, 3 Sep 2008 11:59:59 -0400 > From: NHeath@Lifespan.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] What to do when there is no liquid nitrogen :p > > Hi Everyone, > > Does anyone have alternate methods for snap freezing muscle when there > is no liquid nitrogen?? I've been told to make a slurry with isopentane > & dry ice or to put isopentane in a plastic cup (?) and put into the > minus 70* freezer till it gets cold. Also how volatile / dangerous is > isopentane to work with??? Other than a flammable cabinet are there any > other things I need to know about isopentane??? > > Thanks, > Nancy > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Stay up to date on your PC, the Web, and your mobile phone with Windows Live. http://clk.atdmt.com/MRT/go/msnnkwxp1020093185mrt/direct/01/ From brian.chelack <@t> pds.usask.ca Wed Sep 3 12:09:35 2008 From: brian.chelack <@t> pds.usask.ca (Brian Chelack) Date: Wed Sep 3 12:09:41 2008 Subject: [Histonet] Looking for a source for Fisher Code-On absorbent pads Message-ID: <48A69EFF0CFF4F85990A403045827808@usask.ca> We are having difficulty sourcing the absorbent pads for our old (very very old) Fisher Code-On immunostainer. These absorbent pads are 31/2 X31/2 X 1 inch thick. I would like to keep this old relic going for a few more years (it still is one of the cheapest immunostainers to operate and is well suited to running large numbers of a single stain at a time. Our Thermo Fisher rep is only able to obtain the newer and smaller pads which are not suitable. Does anyone else still use these pads and if so can you tell me where you get them? Otherwise I will have to convert a great many surveillance protocols over to our Ventana equipment. Happily stuck in the IHC stone age, Brian Chelack Special Projects Manager Prairie Diagnostic Services Inc. 52 Campus Drive, Saskatoon, SK S7N 5B4 Phone (306) 966-7211 Fax (306) 966-7244 Email brian.chelack@pds.usask.ca Confidentiality Notice: The information contained in this message and/or attachments may contain confidential and/or privileged information that is intended for the persons or entities addressed above. Any disclosure, copying, retransmission or dissemination is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy the message. From nancy.troiano <@t> yale.edu Wed Sep 3 12:11:29 2008 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Wed Sep 3 12:11:35 2008 Subject: [Histonet] NSH conference and Plastics Message-ID: <5.2.1.1.2.20080903130847.021de408@email.med.yale.edu> Hi Suzanne - a great place to meet others who work with plastics like myself is at the Hard Tissue Society meeting which will be held around mid-day on Saturday, Sept. 13th. Also, stop by the table set up by the Hard Tissue society. From brian.chelack <@t> pds.usask.ca Wed Sep 3 12:20:22 2008 From: brian.chelack <@t> pds.usask.ca (Brian Chelack) Date: Wed Sep 3 12:20:30 2008 Subject: [Histonet] Re: What to do when there is no liquid nitrogen Message-ID: <7E2899A216564F95B375D2C8BEF76E03@usask.ca> Rather than fussing with iso-pentane in your -70 freezer you could just place a metal block in the freezer for a few hours and freeze your tissues in a small mold placed directly on top of it. The metal block will freeze the tissues in a minute or so. I have done this with small biopsy materials in OCT and have found the results to be adequate. I rarely have problems with cracked OCT blocks that can occur when using liquid nitrogen. The key is to keep the sample material as small as possible to allow for quick freezing. You should run a small trial of normally frozen materials vs. the alternate method before implementing. Brian Chelack Special Projects Manager Prairie Diagnostic Services Inc. 52 Campus Drive, Saskatoon, SK S7N 5B4 Phone (306) 966-7211 Fax (306) 966-7244 Email brian.chelack@pds.usask.ca Confidentiality Notice: The information contained in this message and/or attachments may contain confidential and/or privileged information that is intended for the persons or entities addressed above. Any disclosure, copying, retransmission or dissemination is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy the message. From bob.nienhuis <@t> gmail.com Wed Sep 3 12:22:35 2008 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Wed Sep 3 12:22:41 2008 Subject: [Histonet] What to do when there is no liquid nitrogen :p In-Reply-To: <130E8991F210424096EFC6F42EA33B240337B930@LSCOEXCH1.lsmaster.lifespan.org> References: <130E8991F210424096EFC6F42EA33B240337B930@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: <45109da50809031022l3653c81dv5cd02e8930ddbd10@mail.gmail.com> At our last safety class they showed pictures of a couple of blown-up hoods and cabinets caused by exploding isopentane. Especially dangerous when there is only a small amount in the bottle so that vapors can form. Flash point in near room temperature. Bob On 9/3/08, Heath, Nancy L. wrote: > > Hi Everyone, > > Does anyone have alternate methods for snap freezing muscle when there > is no liquid nitrogen?? I've been told to make a slurry with isopentane > & dry ice or to put isopentane in a plastic cup (?) and put into the > minus 70* freezer till it gets cold. Also how volatile / dangerous is > isopentane to work with??? Other than a flammable cabinet are there any > other things I need to know about isopentane??? > > Thanks, > Nancy > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kalschev <@t> svm.vetmed.wisc.edu Wed Sep 3 12:27:47 2008 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Wed Sep 3 12:28:58 2008 Subject: [Histonet] Hard Tissue Group - Suzanne Bruce and other's welcome Message-ID: <004001c90dea$62096120$c5d76880@vetmed.wisc.edu> Suzanne and other interested attendees: The National Society for Histotechnology Hard Tissue Committee is meeting in Pittsburgh on Saturday, September 13th; room 413/415, of the David L. Lawrence Convention Center. The time is 12:00 p.m. - 12:45 p.m. We welcome new members. This is a good place to meet others working in plastics. There is also a Hard Tissue Display in the registration area with materials and application. It is often a gathering spot for attendees involved in this specialty, from Saturday, September 13 - Tuesday morning, September 16th. I look forward to meeting you. Vicki Kalscheur, Hard Tissue Committee Chair Department of Surgical Sciences School of Veterinary Medicine University of Wisconsin 2015 Linden Drive Madison, WI 53706-1102 Phone: 608-262-8534 FAX: 608-263-7930 kalschev@svm.vetmed.wisc.edu www.vetmed.wisc.edu/research/orthop From burch007 <@t> mc.duke.edu Wed Sep 3 12:30:34 2008 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Wed Sep 3 12:30:48 2008 Subject: [Histonet] Looking for a source for Fisher Code-On absorbent pads In-Reply-To: <48A69EFF0CFF4F85990A403045827808@usask.ca> Message-ID: Brian: Always willing to help out an old cap gappper! We still use a manual cap gap method. The Code On pads can be ordered from CCP Industries. Catalog # 7151872. Contact Eilene Foss at 1-866-431-4227, ext. 234 or efoss@ccpind.com to make sure you get the correct size. My last price was $96.72 a case. I think they offer free shipping with an order of 6 cases. Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" Brian Chelack Sent by: histonet-bounces@lists.utsouthwestern.edu 09/03/2008 01:12 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Looking for a source for Fisher Code-On absorbent pads We are having difficulty sourcing the absorbent pads for our old (very very old) Fisher Code-On immunostainer. These absorbent pads are 31/2 X31/2 X 1 inch thick. I would like to keep this old relic going for a few more years (it still is one of the cheapest immunostainers to operate and is well suited to running large numbers of a single stain at a time. Our Thermo Fisher rep is only able to obtain the newer and smaller pads which are not suitable. Does anyone else still use these pads and if so can you tell me where you get them? Otherwise I will have to convert a great many surveillance protocols over to our Ventana equipment. Happily stuck in the IHC stone age, Brian Chelack Special Projects Manager Prairie Diagnostic Services Inc. 52 Campus Drive, Saskatoon, SK S7N 5B4 Phone (306) 966-7211 Fax (306) 966-7244 Email brian.chelack@pds.usask.ca Confidentiality Notice: The information contained in this message and/or attachments may contain confidential and/or privileged information that is intended for the persons or entities addressed above. Any disclosure, copying, retransmission or dissemination is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lac65 <@t> email.med.yale.edu Wed Sep 3 12:39:26 2008 From: lac65 <@t> email.med.yale.edu (Lori Charette) Date: Wed Sep 3 12:37:43 2008 Subject: [Histonet] Re: Histonet Digest, Vol 58, Issue 3 In-Reply-To: <200809031700.m83H0pg7020320@mr5.its.yale.edu> References: <200809031700.m83H0pg7020320@mr5.its.yale.edu> Message-ID: <48BECBCE.4080909@email.med.yale.edu> Hi Jim, There is an interesting paper that may help. It discusses nasal lavages, tissue eosinophils, and immuno. Here is the link. The title of the paper is Eosinophil degranulation status in allergic rhinitis: observations before and during seasonal allergen exposure. http://www.erj.ersjournals.com/cgi/content/full/24/5/750 ~Lori histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Michelle Steinkrauss is out of the office. > (michelle.steinkrauss@novartis.com) > 2. RE: Documenting control tissue source? (Cheri Miller) > 3. Job opening (Rathborne, Toni) > 4. NSH Conference (Suzanne Bruce) > 5. Differentiating a neutrophil from a de-granulated eosinophil > ....antibody or stain? (jstaruk) > 6. Re: Differentiating a neutrophil from a de-granulated > eosinophil ....antibody or stain? (Mark Tarango) > 7. BUGZ (Amos Brooks) > 8. Histology Position Available - Baltimore Maryland > (LAURA KORCZYNSKI) > 9. IHC buffer expiration date (Jan Shivers) > 10. Hello (Lori Charette) > 11. Re: Hello (Pat Flannery) > 12. What to do when there is no liquid nitrogen :p (Heath, Nancy L.) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 2 Sep 2008 13:36:24 -0400 > From: michelle.steinkrauss@novartis.com > Subject: [Histonet] Michelle Steinkrauss is out of the office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=US-ASCII > > > I will be out of the office starting 09/02/2008 and will not return until > 09/03/2008. > > If you require immediate assistance for study-related matters, please > contact Michelle Broome at x 47477. For clinical pathology assistance, > please contact Rhonda Osborne at x 47099. > > > > > ------------------------------ > > Message: 2 > Date: Tue, 2 Sep 2008 14:05:48 -0500 > From: "Cheri Miller" > Subject: RE: [Histonet] Documenting control tissue source? > To: "'Tom McNemar'" , > > Message-ID: <00a101c90d2e$e95fdbc0$3d02a8c0@plab.local> > Content-Type: text/plain; charset="us-ascii" > > I agree with you. We just had our inspection in July and it wasn't an issue. > > > Cheryl Miller HT (ASCP) > Histology Supervisor > Physicians Laboratory,P.C. > Omaha, Ne. > 402 738 5052 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar > Sent: Friday, August 15, 2008 6:12 AM > To: histonet@pathology.swmed.edu > Subject: [Histonet] Documenting control tissue source? > > We are finishing up our JCAHO inspection today. The inspector asked about > our control tissues and where we documented the source. We get nearly all > of our controls from patient samples and she said that most histo labs mark > their control blocks with the source case number. They then keep records of > the control source used when staining new cases. I can see no logical > reason to keep this info. You use a control appropriate for the element > being stained. Why does it matter where the control came from? Am I wrong? > Do you do this? > > Thanks. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you have > received this message in error, please notify the sender immediately and > delete this email from your system. > > > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 2 Sep 2008 15:26:52 -0400 > From: "Rathborne, Toni" > Subject: [Histonet] Job opening > To: > Message-ID: > > > Content-Type: text/plain; charset="utf-8" > > > Somerset Medical Center, located in central NJ, has a daytime opening for a FT Histotech. Embedding, microtomy, knowledge of special stains and IHC required. Apply online at http://somersetmedicalcenter.com/, or send resume to: > > trathborne@somerset-healthcare.com > > Toni Rathborne > Pathology Supervisor > Somerset Medical Center > 110 Rehill Ave. > Somerville,NJ 08876 > 908-595-2367 > > > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > ------------------------------ > > Message: 4 > Date: Tue, 2 Sep 2008 16:51:40 -0400 > From: "Suzanne Bruce" > Subject: [Histonet] NSH Conference > To: "histonet" > Message-ID: > <26DB1FDFBF9EE14AB3AACC873519A4A2515149@vpss1.VetPathServicesInc.local> > > Content-Type: text/plain; charset="iso-8859-1" > > Hello, this message is mainly for the boneheads and plastics users (specifically Technovit 7200, GMA and others using the Exakt system). I will be attending the NSH conference for the first time. I don't know many people involved in plastics, so I was wondering who else would be attending that is familiar w/the plastics I mentioned above. I'd certainly like to meet a few of you! Also any friendly words of advice about getting the most out of the conference would be appreciated! > > Thanks so much! > ________________________________________ > Suzanne Bruce, R.V.T. > Histologist / Necropsy Coordinator > Vet Path Services, Inc. (VPS) > 6450 Castle Dr. > Mason, OH 45040 > Lab: (513) 469-0777 ext 19 > Fax: (513) 469-2474 > Email: sbruce@vetpathservicesinc.com > www.vetpathservicesinc.com > > > > ------------------------------ > > Message: 5 > Date: Tue, 2 Sep 2008 20:26:31 -0400 > From: "jstaruk" > Subject: [Histonet] Differentiating a neutrophil from a de-granulated > eosinophil ....antibody or stain? > To: "'histonet'" > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Hi all, > > Does anyone know if the anti-eosinophil antibody will adhere to a > degranulated eosinophil or does the antibody only stick to the eosinophil > granules and not the cell itself? > > I'm performing a differential count in a certain fluid and I'm highly > suspicious that many of the neutrophils are actually de-granulated > eosinophils (a bit larger, thicker, less segmented nuclei, etc). Is there > any other method to differentiate a neutrophils from a degranulated > eosinophil that I'm not aware of? > > Thank you! > > Jim > > _____________________ > Jim Staruk > Mass Histology Service > www.masshistology.com > > > > ------------------------------ > > Message: 6 > Date: Tue, 2 Sep 2008 17:32:14 -0700 > From: "Mark Tarango" > Subject: Re: [Histonet] Differentiating a neutrophil from a > de-granulated eosinophil ....antibody or stain? > To: jstaruk > Cc: histonet > Message-ID: > <5b6eb13e0809021732j11d1c969nc41921d7f3628091@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Got a picture? > > On 9/2/08, jstaruk wrote: > >> Hi all, >> >> Does anyone know if the anti-eosinophil antibody will adhere to a >> degranulated eosinophil or does the antibody only stick to the eosinophil >> granules and not the cell itself? >> >> I'm performing a differential count in a certain fluid and I'm highly >> suspicious that many of the neutrophils are actually de-granulated >> eosinophils (a bit larger, thicker, less segmented nuclei, etc). Is there >> any other method to differentiate a neutrophils from a degranulated >> eosinophil that I'm not aware of? >> >> Thank you! >> >> Jim >> >> _____________________ >> Jim Staruk >> Mass Histology Service >> www.masshistology.com >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > ------------------------------ > > Message: 7 > Date: Wed, 3 Sep 2008 10:24:47 -0400 > From: "Amos Brooks" > Subject: [Histonet] BUGZ > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <582736990809030724p3a842a4fscedd9fc487f35c21@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hi, > Here's an oddball question. I have a researcher that wants to bring me > mosquitoes for paraffin sectioning. I immediatly had a chill when thinking > of cutting the chitin. Has anyone worked with similar bugs, and if so how > did you process them to make them cuttable? What fixative would be best? > > Thanks, > Amos > > > ------------------------------ > > Message: 8 > Date: Wed, 03 Sep 2008 10:26:11 -0400 > From: "LAURA KORCZYNSKI" > Subject: [Histonet] Histology Position Available - Baltimore Maryland > To: > Message-ID: <48BE6640.B3F2.00C2.0@gbmc.org> > Content-Type: text/plain; charset=US-ASCII > > > > HISTOTECHNOLOGIST > Full Time -Day Shift/1 Saturday Every 6 weeks > > Fully automated histology team is looking for an experienced individual that is ASCP registered or is eligible. At least 2 years histology experience is desired. Duties include embedding, cutting, H&E and Histochemical Staining of tissue specimens. Free on-site parking. Qualified candidates can apply on-line to GBMC.org and/or send resume to LKorczyn@gbmc.org. > > > > > Laura Korczynski > > Histology Supervisor > Greater Baltimore Medical Center > Phone: 443-849-2831 > Fax: 443-849-3016 > > _______________________________________________________________________________________ > > This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. > > > > > ------------------------------ > > Message: 9 > Date: Wed, 3 Sep 2008 09:49:20 -0500 > From: "Jan Shivers" > Subject: [Histonet] IHC buffer expiration date > To: "histonet" > Message-ID: <3FEAA312E14E47989C362072225F5E04@auxs.umn.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Hi folks, > > What is the general concensus on expiration dates of IHC buffers that one prepares from scratch and keeps at room temperature (TBS, Citrate, etc.)? I've found one source that states 3 months as a storage time period. Is this the general assumption or do you use a different timeline? > > Jan Shivers > Histology/IHC/EM > University of Minnesota > > > ------------------------------ > > Message: 10 > Date: Wed, 03 Sep 2008 11:09:48 -0400 > From: Lori Charette > Subject: [Histonet] Hello > To: histonet@lists.utsouthwestern.edu > Message-ID: <48BEA8BC.1070508@email.med.yale.edu> > Content-Type: text/plain; format=flowed; charset=ISO-8859-1 > > Hello > > -- Lori A. Charette, MSM, MT, HT (ASCP) Manager, Research Histology / Tissue Microarray Facility 310 Cedar St. LH-202 New Haven, CT 06510 phone (203)737-4198 fax (203) 737-1198 From lac65 <@t> email.med.yale.edu Wed Sep 3 13:27:50 2008 From: lac65 <@t> email.med.yale.edu (Lori Charette) Date: Wed Sep 3 13:26:01 2008 Subject: [Histonet] BUGZ In-Reply-To: <200809031700.m83H0pg7020320@mr5.its.yale.edu> References: <200809031700.m83H0pg7020320@mr5.its.yale.edu> Message-ID: <48BED726.8060503@email.med.yale.edu> Hi Amos, You can decalcify with hydrochloric acid with a chelating agent like EDTA. 0.5M EDTA pH 8.0 (GIBCO) 30ml ddH2O 70ml 6N HCL 1.2ml pH 6.4-6.8 1. Wash fixed tissue with PBS 20min X3. 2. Wash tissue with ddH2O 20min X3. 3. Change tissue to EDTA solution with agitation at RT for 2-3 days depend on tissue size. 4. Change to fresh EDTA solution if need further decalcification. 5. Rinse with ddH2O 20min X3. 6. Wash with ddH2O 20min X3. 7. Dehydration and embedding I did not see much in the insect world but there is some info in the invertebrate world. Lots of exoskeleton and chitin there. Hope this helps. Lori histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Michelle Steinkrauss is out of the office. > (michelle.steinkrauss@novartis.com) > 2. RE: Documenting control tissue source? (Cheri Miller) > 3. Job opening (Rathborne, Toni) > 4. NSH Conference (Suzanne Bruce) > 5. Differentiating a neutrophil from a de-granulated eosinophil > ....antibody or stain? (jstaruk) > 6. Re: Differentiating a neutrophil from a de-granulated > eosinophil ....antibody or stain? (Mark Tarango) > 7. BUGZ (Amos Brooks) > 8. Histology Position Available - Baltimore Maryland > (LAURA KORCZYNSKI) > 9. IHC buffer expiration date (Jan Shivers) > 10. Hello (Lori Charette) > 11. Re: Hello (Pat Flannery) > 12. What to do when there is no liquid nitrogen :p (Heath, Nancy L.) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 2 Sep 2008 13:36:24 -0400 > From: michelle.steinkrauss@novartis.com > Subject: [Histonet] Michelle Steinkrauss is out of the office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=US-ASCII > > > I will be out of the office starting 09/02/2008 and will not return until > 09/03/2008. > > If you require immediate assistance for study-related matters, please > contact Michelle Broome at x 47477. For clinical pathology assistance, > please contact Rhonda Osborne at x 47099. > > > > > ------------------------------ > > Message: 2 > Date: Tue, 2 Sep 2008 14:05:48 -0500 > From: "Cheri Miller" > Subject: RE: [Histonet] Documenting control tissue source? > To: "'Tom McNemar'" , > > Message-ID: <00a101c90d2e$e95fdbc0$3d02a8c0@plab.local> > Content-Type: text/plain; charset="us-ascii" > > I agree with you. We just had our inspection in July and it wasn't an issue. > > > Cheryl Miller HT (ASCP) > Histology Supervisor > Physicians Laboratory,P.C. > Omaha, Ne. > 402 738 5052 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar > Sent: Friday, August 15, 2008 6:12 AM > To: histonet@pathology.swmed.edu > Subject: [Histonet] Documenting control tissue source? > > We are finishing up our JCAHO inspection today. The inspector asked about > our control tissues and where we documented the source. We get nearly all > of our controls from patient samples and she said that most histo labs mark > their control blocks with the source case number. They then keep records of > the control source used when staining new cases. I can see no logical > reason to keep this info. You use a control appropriate for the element > being stained. Why does it matter where the control came from? Am I wrong? > Do you do this? > > Thanks. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you have > received this message in error, please notify the sender immediately and > delete this email from your system. > > > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 2 Sep 2008 15:26:52 -0400 > From: "Rathborne, Toni" > Subject: [Histonet] Job opening > To: > Message-ID: > > > Content-Type: text/plain; charset="utf-8" > > > Somerset Medical Center, located in central NJ, has a daytime opening for a FT Histotech. Embedding, microtomy, knowledge of special stains and IHC required. Apply online at http://somersetmedicalcenter.com/, or send resume to: > > trathborne@somerset-healthcare.com > > Toni Rathborne > Pathology Supervisor > Somerset Medical Center > 110 Rehill Ave. > Somerville,NJ 08876 > 908-595-2367 > > > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > ------------------------------ > > Message: 4 > Date: Tue, 2 Sep 2008 16:51:40 -0400 > From: "Suzanne Bruce" > Subject: [Histonet] NSH Conference > To: "histonet" > Message-ID: > <26DB1FDFBF9EE14AB3AACC873519A4A2515149@vpss1.VetPathServicesInc.local> > > Content-Type: text/plain; charset="iso-8859-1" > > Hello, this message is mainly for the boneheads and plastics users (specifically Technovit 7200, GMA and others using the Exakt system). I will be attending the NSH conference for the first time. I don't know many people involved in plastics, so I was wondering who else would be attending that is familiar w/the plastics I mentioned above. I'd certainly like to meet a few of you! Also any friendly words of advice about getting the most out of the conference would be appreciated! > > Thanks so much! > ________________________________________ > Suzanne Bruce, R.V.T. > Histologist / Necropsy Coordinator > Vet Path Services, Inc. (VPS) > 6450 Castle Dr. > Mason, OH 45040 > Lab: (513) 469-0777 ext 19 > Fax: (513) 469-2474 > Email: sbruce@vetpathservicesinc.com > www.vetpathservicesinc.com > > > > ------------------------------ > > Message: 5 > Date: Tue, 2 Sep 2008 20:26:31 -0400 > From: "jstaruk" > Subject: [Histonet] Differentiating a neutrophil from a de-granulated > eosinophil ....antibody or stain? > To: "'histonet'" > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Hi all, > > Does anyone know if the anti-eosinophil antibody will adhere to a > degranulated eosinophil or does the antibody only stick to the eosinophil > granules and not the cell itself? > > I'm performing a differential count in a certain fluid and I'm highly > suspicious that many of the neutrophils are actually de-granulated > eosinophils (a bit larger, thicker, less segmented nuclei, etc). Is there > any other method to differentiate a neutrophils from a degranulated > eosinophil that I'm not aware of? > > Thank you! > > Jim > > _____________________ > Jim Staruk > Mass Histology Service > www.masshistology.com > > > > ------------------------------ > > Message: 6 > Date: Tue, 2 Sep 2008 17:32:14 -0700 > From: "Mark Tarango" > Subject: Re: [Histonet] Differentiating a neutrophil from a > de-granulated eosinophil ....antibody or stain? > To: jstaruk > Cc: histonet > Message-ID: > <5b6eb13e0809021732j11d1c969nc41921d7f3628091@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Got a picture? > > On 9/2/08, jstaruk wrote: > >> Hi all, >> >> Does anyone know if the anti-eosinophil antibody will adhere to a >> degranulated eosinophil or does the antibody only stick to the eosinophil >> granules and not the cell itself? >> >> I'm performing a differential count in a certain fluid and I'm highly >> suspicious that many of the neutrophils are actually de-granulated >> eosinophils (a bit larger, thicker, less segmented nuclei, etc). Is there >> any other method to differentiate a neutrophils from a degranulated >> eosinophil that I'm not aware of? >> >> Thank you! >> >> Jim >> >> _____________________ >> Jim Staruk >> Mass Histology Service >> www.masshistology.com >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > ------------------------------ > > Message: 7 > Date: Wed, 3 Sep 2008 10:24:47 -0400 > From: "Amos Brooks" > Subject: [Histonet] BUGZ > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <582736990809030724p3a842a4fscedd9fc487f35c21@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hi, > Here's an oddball question. I have a researcher that wants to bring me > mosquitoes for paraffin sectioning. I immediatly had a chill when thinking > of cutting the chitin. Has anyone worked with similar bugs, and if so how > did you process them to make them cuttable? What fixative would be best? > > Thanks, > Amos > > > ------------------------------ > > Message: 8 > Date: Wed, 03 Sep 2008 10:26:11 -0400 > From: "LAURA KORCZYNSKI" > Subject: [Histonet] Histology Position Available - Baltimore Maryland > To: > Message-ID: <48BE6640.B3F2.00C2.0@gbmc.org> > Content-Type: text/plain; charset=US-ASCII > > > > HISTOTECHNOLOGIST > Full Time -Day Shift/1 Saturday Every 6 weeks > > Fully automated histology team is looking for an experienced individual that is ASCP registered or is eligible. At least 2 years histology experience is desired. Duties include embedding, cutting, H&E and Histochemical Staining of tissue specimens. Free on-site parking. Qualified candidates can apply on-line to GBMC.org and/or send resume to LKorczyn@gbmc.org. > > > > > Laura Korczynski > > Histology Supervisor > Greater Baltimore Medical Center > Phone: 443-849-2831 > Fax: 443-849-3016 > > _______________________________________________________________________________________ > > This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. > > > > > ------------------------------ > > Message: 9 > Date: Wed, 3 Sep 2008 09:49:20 -0500 > From: "Jan Shivers" > Subject: [Histonet] IHC buffer expiration date > To: "histonet" > Message-ID: <3FEAA312E14E47989C362072225F5E04@auxs.umn.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Hi folks, > > What is the general concensus on expiration dates of IHC buffers that one prepares from scratch and keeps at room temperature (TBS, Citrate, etc.)? I've found one source that states 3 months as a storage time period. Is this the general assumption or do you use a different timeline? > > Jan Shivers > Histology/IHC/EM > University of Minnesota > > > ------------------------------ > > Message: 10 > Date: Wed, 03 Sep 2008 11:09:48 -0400 > From: Lori Charette > Subject: [Histonet] Hello > To: histonet@lists.utsouthwestern.edu > Message-ID: <48BEA8BC.1070508@email.med.yale.edu> > Content-Type: text/plain; format=flowed; charset=ISO-8859-1 > > Hello > > -- Lori A. Charette, MSM, MT, HT (ASCP) Manager, Research Histology / Tissue Microarray Facility 310 Cedar St. LH-202 New Haven, CT 06510 phone (203)737-4198 fax (203) 737-1198 From laurie.colbert <@t> huntingtonhospital.com Wed Sep 3 13:40:28 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Sep 3 13:40:33 2008 Subject: [Histonet] Pituitary Control Tissue Message-ID: <57BE698966D5C54EAE8612E8941D768303ACC84F@EXCHANGE3.huntingtonhospital.com> Does anyone know where I can purchase pituitary tissue for IHC controls? Laurie From katelin <@t> cuttingedgehistology.com Wed Sep 3 13:41:32 2008 From: katelin <@t> cuttingedgehistology.com (Katelin Lester) Date: Wed Sep 3 13:41:37 2008 Subject: [Histonet] Cornea Processing and Cutting Message-ID: <000e01c90df4$afbb0360$4010a8c0@Front> I am working, for the first time, with monkey cornea and I am hoping for some advice on how many microns to cut the tissue at. Also, these are paraffin embedded sections and we are working on the best way to process them. Any knowledge you have would be greatly appreciated! Katelin Lester Cutting Edge Histology Services, LLC From tere.insausti <@t> univ-tours.fr Wed Sep 3 14:01:18 2008 From: tere.insausti <@t> univ-tours.fr (Teresita Insausti) Date: Wed Sep 3 14:01:36 2008 Subject: [Histonet] BUGZ In-Reply-To: <582736990809030724p3a842a4fscedd9fc487f35c21@mail.gmail.com> References: <582736990809030724p3a842a4fscedd9fc487f35c21@mail.gmail.com> Message-ID: <8BBBAB362ED348BA82B73DF2F8C7CFAC@PCdeTere> Hi Amos, I have a long experience working with insects and the best way to cut cuticle is to include the sample in plastic resin, the same as used in preparations for electronic microscopy (Araldite or Durcupan). The hardness of the paraffin is very different to that of cuticle and as a consequence the cuts break down. The only problem of using resin is that it does not allow certain coloration methods, but for a general picture it is very good. As for the fixer, it depends on the tissue that you want to study, and with which details. For general purposes the mixture alcohol - formaldehyde- acetic acid can works well. If you are interested, I can send to you the detailled protocols. Teresita Dr. Teresita Insausti Institut de Recherche sur la Biologie de l'Insecte (IRBI) Universit? Fran?ois Rabelais - Parc Grandmont 37200 Tours France e-mail: tere.insausti@univ-tours.fr Phone: +33 (0)2 47 36 73 66 Fax: +33 (0)2 47 36 69 66 http://www.univ-tours.fr/irbi/ ----- Original Message ----- From: "Amos Brooks" To: Sent: Wednesday, September 03, 2008 4:24 PM Subject: [Histonet] BUGZ > Hi, > Here's an oddball question. I have a researcher that wants to bring me > mosquitoes for paraffin sectioning. I immediatly had a chill when thinking > of cutting the chitin. Has anyone worked with similar bugs, and if so how > did you process them to make them cuttable? What fixative would be best? > > Thanks, > Amos > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.169 / Virus Database: 270.6.15/1649 - Release Date: 03/09/2008 07:15 From sjchtascp <@t> yahoo.com Wed Sep 3 14:39:16 2008 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Wed Sep 3 14:39:20 2008 Subject: [Histonet] Looking for work Madison, WI Message-ID: <631962.5160.qm@web38208.mail.mud.yahoo.com> Looking for HT opportunities in Madison, WI.?? Temp work in Milwaulee, WI? 3-4 week temp anywhere in the US ? Thanks everyone, ? Steve From marktarango <@t> gmail.com Wed Sep 3 16:58:53 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Sep 3 16:58:58 2008 Subject: [Histonet] Hello In-Reply-To: <6EEF0B68-5BD2-482E-A13B-87C71F995155@duke.edu> References: <48BEA8BC.1070508@email.med.yale.edu> <6EEF0B68-5BD2-482E-A13B-87C71F995155@duke.edu> Message-ID: <5b6eb13e0809031458m26c556efx2addbb7edaa11a0f@mail.gmail.com> Hey nothing's wrong w/ just saying hi.... hehe On 9/3/08, Pat Flannery wrote: > Well, hello right back, Lori! Nice to hear from you, but did you have a > question or suggestion for the group? :-) > > -Pat Flannery (Duke wise guy) > > > On Sep 3, 2008, at 11:09 AM, Lori Charette wrote: > > > Hello > > > > -- > > Lori A. Charette, MSM, MT, HT (ASCP) > > Manager, Research Histology / Tissue Microarray Facility > > 310 Cedar St. > > LH-202 > > New Haven, CT 06510 > > phone (203)737-4198 > > fax (203) 737-1198 > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sprin119 <@t> umn.edu Wed Sep 3 17:59:52 2008 From: sprin119 <@t> umn.edu (Jennifer Springsteen) Date: Wed Sep 3 18:04:25 2008 Subject: [Histonet] Luciferase detection in frozen sections Message-ID: Can anyone recommend a good protocol/product to detect luciferase in frozen sections? I've seen lots of recommendations for antibodies in the archives, but I'm wondering if there is reliable, relatively simple enzymatic kit available. Or, if anyone has a tried and true antibody protocol, that would be great, too! Thanks! -- Jennifer L. Springsteen, B.S. Lab Manager, Assistant Scientist Lillehei Heart Institute Molecular Histopathology Research Facility University of Minnesota Medical School, Division of Cardiology 4-266 Nils Hasselmo Hall 312 Church Street SE Minneapolis, MN 55455 Lab: 612-626-3090 Cell: 651-357-7916 Fax: 612-624-8118 From D.E.Hilling <@t> lumc.nl Thu Sep 4 05:58:06 2008 From: D.E.Hilling <@t> lumc.nl (D.E.Hilling@lumc.nl) Date: Thu Sep 4 05:58:16 2008 Subject: [Histonet] anti-collagenase staining paraffine tissue Message-ID: Does anybody have any experience with anti-collagenase staining on paraffine sections? In frozen sections we get a sensible staining pattern (the way we expected it to be), but we have problems transferring this to paraffine sections of the same tissue (human pancreasses), were the staining seems to be less intense and atypical (despite changing concentrations, duration). I reckon, it might be a cross-linking problem (due to the formaline fixation en paraffine embedding??) ..... does anybody know if (and how) I can get rid of these or maybe have other suggestions? Thanks! Denise Hilling Denise Hilling MD PhD student Department of Surgery Leiden University Medical Center The Netherlands From NHeath <@t> Lifespan.org Thu Sep 4 06:08:23 2008 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Thu Sep 4 06:08:29 2008 Subject: [Histonet] Freezing W/O Liquid Nitrogen...THANKS EVERYONE :) Message-ID: <130E8991F210424096EFC6F42EA33B240337B9B8@LSCOEXCH1.lsmaster.lifespan.org> Hi, Thanks to all who answered my questions about my problem with having no liquid nitrogen. I have made a special folder to keep everyones replies in so I have a reference to go to if this ever happens again....OMG I hope it doesn't happen again that day was a fiasco!! Thanks again :) Nancy From lab.mef <@t> smmc.org Thu Sep 4 06:44:00 2008 From: lab.mef <@t> smmc.org (Meredith Fuller-Fedorczyk) Date: Thu Sep 4 06:44:11 2008 Subject: [Histonet] What to do when there is no liquid nitrogen :p References: <130E8991F210424096EFC6F42EA33B240337B930@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: <00da01c90e83$85ed0ee0$240c0180@PCLAB11> We send our muscle biopsies to Mayo Labs and they recommend 100% alcohol or Acetone with dry ice for the slurry. It than can be stored in the -70 degree freezer. Isopentane is just so risky to store. ----- Original Message ----- From: "Heath, Nancy L." To: Sent: Wednesday, September 03, 2008 11:59 AM Subject: [Histonet] What to do when there is no liquid nitrogen :p Hi Everyone, Does anyone have alternate methods for snap freezing muscle when there is no liquid nitrogen?? I've been told to make a slurry with isopentane & dry ice or to put isopentane in a plastic cup (?) and put into the minus 70* freezer till it gets cold. Also how volatile / dangerous is isopentane to work with??? Other than a flammable cabinet are there any other things I need to know about isopentane??? Thanks, Nancy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Thu Sep 4 08:52:31 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Sep 4 08:52:39 2008 Subject: [Histonet] RE: What to do when there is no liquid nitrogen :p In-Reply-To: <130E8991F210424096EFC6F42EA33B240337B930@LSCOEXCH1.lsmaster.lifespan.org> References: <130E8991F210424096EFC6F42EA33B240337B930@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: Unless your ultra low freezer is rated explosion proof, absolutely do NOT put isopentane in your freezer. In May 2003 HistoLogic featured an article about a lab that sustained about $250,000 in damages resulting from a researcher doing this very thing. There is a potential loss of life should individuals be working in a lab during such event. If you wish to read this article or show it to those suggesting you use isopentane in the freezer, you can find it at this link http://www.sakura-americas.com/histologic/pdf/03_may.pdf Secondly, freezing at -70 degrees C is not considered adequate for avoiding freeze artifact (ice crystal artifact) in skeletal muscle samples. Liquid nitrogen chilled isopentane freezing occurs at about -150 degrees C Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heath, Nancy L. Sent: Wednesday, September 03, 2008 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What to do when there is no liquid nitrogen :p Hi Everyone, Does anyone have alternate methods for snap freezing muscle when there is no liquid nitrogen?? I've been told to make a slurry with isopentane & dry ice or to put isopentane in a plastic cup (?) and put into the minus 70* freezer till it gets cold. Also how volatile / dangerous is isopentane to work with??? Other than a flammable cabinet are there any other things I need to know about isopentane??? Thanks, Nancy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stema_cba <@t> yahoo.it Thu Sep 4 09:25:23 2008 From: stema_cba <@t> yahoo.it (Stefano Mantero) Date: Thu Sep 4 09:25:34 2008 Subject: [Histonet] Leica TP1020 Message-ID: <475336.45675.qm@web23105.mail.ird.yahoo.com> Hi, My institute has acquired a Leica Tissue Processor TP1020. Unfortunately this instrument sometimes gives to us of the inclusion problems: badly dehydrated samples or a excessive drying. You have some suggestions in order to help me to install the system? We work with mouse tissue fixed with 10% Formalin or 4% PFA. Many Thanks in advance Stefano Mantero __________________________________________________ Do You Yahoo!? Poco spazio e tanto spam? Yahoo! Mail ti protegge dallo spam e ti da tanto spazio gratuito per i tuoi file e i messaggi http://mail.yahoo.it From dellav <@t> musc.edu Thu Sep 4 09:29:19 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Sep 4 09:29:37 2008 Subject: [Histonet] Histotech to be pregnant with questions In-Reply-To: References: Message-ID: I didn't see any responses to Julie's question on the list but she may have been contacted back channel. The problem is that anecdotal information is just that, and I would suspect that in the absence of formal scientific study, individuals reporting birth effects resulting from lab exposures would fall more into the realm of supposition rather than hard data. To some degree the answer to your question lies in the exposure monitoring levels in your lab. If they have not been tested recently, request that testing be conducted. I'm not certain anyone can comment with certainty regarding risk probabilities. The link below will lead you to an interesting scientific study regarding xylene toxicity that appears comprehensive and specifically mentions histology labs. It may be of interest to others on the list. http://oehha.ca.gov/water/phg/pdf/xylen_c.pdf the following quote comes from this document. "The possibility of fetotoxic effects from concentrations to which humans would be exposed in the workplace seems slight (Brown-Woodman et al., 1991; 1994)." They further state that xylene is not mutagenic or teratogenic. I recommend that you read the entire document however. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Julie Trejo Sent: Wednesday, August 27, 2008 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotech to be pregnant with questions Hi. I'm a histotech about to start on getting pregnant to start our family, yet concerned on the birth defects that come from the xylene fumes. My great co-workers have decided to change processors etc to keep me away from chemicals, which is great for me, just still concerned about the fumes. I would only be embedding and cutting for the most part and we do have alot of automation ie: H&E stainer, Artisian (special stains), and immuno's (BondMax). Automation will keep my hands out of xylene which is wonderful, just worried. I've read some things about histotechs that were pregnant, some ok and some bad (over 20 years ago). Just haven't found out about the recent ones and how their child was. I asked one of our pathologists if it was okay to work in this enviorment but she thinks its okay. I've worked with alot of women whom had children but they never were working in histology when they were pregnant. Basically, I do enjoy my job and don't want to leave where I am, but worry about being pregnant in the histo lab. Is it okay to stay or should I get out or what can I do to stay without harming/risking my baby? I would love to hear from past pregnant mom's in histo lab and let me know if it worked etc. Thanks -- Julie Trejo, HT(ASCP)cm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 4 09:57:05 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 4 09:57:12 2008 Subject: [Histonet] Leica TP1020 In-Reply-To: <475336.45675.qm@web23105.mail.ird.yahoo.com> Message-ID: <406896.90015.qm@web65716.mail.ac4.yahoo.com> Mouse tissue requires LESS dehydration time, you should try to reduce your dehydration steps first of all. Ren? J. --- On Thu, 9/4/08, Stefano Mantero wrote: From: Stefano Mantero Subject: [Histonet] Leica TP1020 To: histonet@lists.utsouthwestern.edu Date: Thursday, September 4, 2008, 10:25 AM Hi, My institute has acquired a Leica Tissue Processor TP1020. Unfortunately this instrument sometimes gives to us of the inclusion problems: badly dehydrated samples or a excessive drying. You have some suggestions in order to help me to install the system? We work with mouse tissue fixed with 10% Formalin or 4% PFA. Many Thanks in advance Stefano Mantero __________________________________________________ Do You Yahoo!? Poco spazio e tanto spam? Yahoo! Mail ti protegge dallo spam e ti da tanto spazio gratuito per i tuoi file e i messaggi http://mail.yahoo.it _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jtrejo2 <@t> slu.edu Thu Sep 4 10:31:20 2008 From: jtrejo2 <@t> slu.edu (Julie Trejo) Date: Thu Sep 4 10:32:51 2008 Subject: [Histonet] Histotech to be pregnant with questions In-Reply-To: References: Message-ID: Thank you for your response and as I thank everyone's wonderfully,overwhelming response. Yes, I have been searching for a long time for the hard data that just couldn't be found by me. I'm not the best at computer searches. I finally had to ask my collegues for their input and I appreciate it greatly. Yes, we are going to check our exposure for formalin and xylene in two ways, badges and the university (enviromental safety). We just started this lab May 5,2008 in sections; H&E routine first, specials second and IHC last until in full swing in August so now would be the best time to test. I have great co-workers who will deal with all the chemicals, so I will just embed and cut and non-chemical things. I may be a over-thinker and worry-wart, but just don't want to risk it for the baby, so "over-kill" will be my nickname for sure. Genetics are something I can't control other than with alot of Folic acid and a healthy diet. Cancergenetic and toxic reagents that I work with, well, that I would like to control the exposure of that. Thank you everyone!!! It calmed me down and gave me food for thought and things to do. I appreciate it. I'll be at NSH 2008 in Pittsburgh. See you there :) Julie 9/4/08, Della Speranza, Vinnie wrote: > I didn't see any responses to Julie's question on the list but she may have > been contacted back channel. The problem is that anecdotal information is > just that, and I would suspect that in the absence of formal scientific > study, individuals reporting birth effects resulting from lab exposures > would fall more into the realm of supposition rather than hard data. > > To some degree the answer to your question lies in the exposure monitoring > levels in your lab. If they have not been tested recently, request that > testing be conducted. > > I'm not certain anyone can comment with certainty regarding risk > probabilities. > > The link below will lead you to an interesting scientific study regarding > xylene toxicity that appears comprehensive and specifically mentions > histology labs. It may be of interest to others on the list. > > http://oehha.ca.gov/water/phg/pdf/xylen_c.pdf > > the following quote comes from this document. > "The possibility of fetotoxic effects from concentrations to which humans > would be exposed in the workplace seems slight (Brown-Woodman et al., 1991; > 1994)." > They further state that xylene is not mutagenic or teratogenic. > I recommend that you read the entire document however. > > > > Vinnie Della Speranza > Manager for Anatomic Pathology Services > 165 Ashley Avenue Suite 309 > Charleston, South Carolina 29425 > Tel: (843) 792-6353 > Fax: (843) 792-8974 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Julie Trejo > Sent: Wednesday, August 27, 2008 3:41 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histotech to be pregnant with questions > > Hi. I'm a histotech about to start on getting pregnant to start our > family, > yet concerned on the birth defects that come from the xylene fumes. My > great co-workers have decided to change processors etc to keep me away from > chemicals, which is great for me, just still concerned about the fumes. I > would only be embedding and cutting for the most part and we do have alot > of > automation ie: H&E stainer, Artisian (special stains), and immuno's > (BondMax). Automation will keep my hands out of xylene which is wonderful, > just worried. I've read some things about histotechs that were pregnant, > some ok and some bad (over 20 years ago). Just haven't found out about the > recent ones and how their child was. I asked one of our pathologists if it > was okay to work in this enviorment but she thinks its okay. I've worked > with alot of women whom had children but they never were working in > histology when they were pregnant. > Basically, I do enjoy my job and don't want to leave where I am, but worry > about being pregnant in the histo lab. Is it okay to stay or should I get > out or what can I do to stay without harming/risking my baby? > I would love to hear from past pregnant mom's in histo lab and let me know > if it worked etc. > > Thanks > > > -- > Julie Trejo, HT(ASCP)cm > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Julie Trejo, HT(ASCP)cm Saint Louis University Dermatology 314-256-3413 From SharonC <@t> celligent.net Thu Sep 4 10:32:44 2008 From: SharonC <@t> celligent.net (Sharon Campbell) Date: Thu Sep 4 10:32:54 2008 Subject: [Histonet] submitting a cytology specimen for FISH Message-ID: Hi everyone, I have a little question about cytology and FISH. We have a client that would like to send a cytology specimen for FISH. Can this specimen be put in a Cytolyt solution? Thanks in advance. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 (704) 549-8444 x104 sharonc@celligent.net From mari.ann.mailhiot <@t> leica-microsystems.com Thu Sep 4 10:36:19 2008 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Thu Sep 4 10:36:46 2008 Subject: [Histonet] Leica TP1020 In-Reply-To: <475336.45675.qm@web23105.mail.ird.yahoo.com> Message-ID: Stefano I will be more than happy to work with you on a protocol for the TP1020. Can you please send me a copy of the protocol you are presently using. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com Stefano Mantero To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Leica TP1020 09/04/2008 09:25 AM Hi, My institute has acquired a Leica Tissue Processor TP1020. Unfortunately this instrument sometimes gives to us of the inclusion problems: badly dehydrated samples or a excessive drying. You have some suggestions in order to help me to install the system? We work with mouse tissue fixed with 10% Formalin or 4% PFA. Many Thanks in advance Stefano Mantero __________________________________________________ Do You Yahoo!? Poco spazio e tanto spam? Yahoo! Mail ti protegge dallo spam e ti da tanto spazio gratuito per i tuoi file e i messaggi http://mail.yahoo.it _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Allison_Scott <@t> hchd.tmc.edu Thu Sep 4 10:41:53 2008 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Thu Sep 4 10:46:55 2008 Subject: [Histonet] Cassette Printers Message-ID: <1872B4A455B7974391609AD8034C79FC082E63@LBEXCH01.hchd.local> Hello to all in histoland. My lab is looking to replace out cassette printer. I would like any opinions on the TBS shur mark if any one is using it. Does it have a integrated computer that does not require a separate pc. Your help in this matter will be greatly appreciated. Allison Scott HT (ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From JWeems <@t> sjha.org Thu Sep 4 11:07:55 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Sep 4 11:08:02 2008 Subject: [Histonet] submitting a cytology specimen for FISH In-Reply-To: References: Message-ID: <982A0A9461F9BF438C7B19A6E425A38358FD2B@ITSSSXM01V6.one.ads.che.org> Would it be a urine for Uryovision? If so, carbowax is generally supplied by the reference lab that does the testing - or PreservCyt will work if you don't have the kits. It must be added in a certain amount of time. This web site tells about it... http://www.urovysion.com/HealthcareProfessionals_7.aspx And if you are sending to a reference lab, you can get kits and specs from them. Hope this helps, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From rjbuesa <@t> yahoo.com Thu Sep 4 11:17:12 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 4 11:17:20 2008 Subject: [Histonet] submitting a cytology specimen for FISH In-Reply-To: <982A0A9461F9BF438C7B19A6E425A38358FD2B@ITSSSXM01V6.one.ads.che.org> Message-ID: <62591.60709.qm@web65707.mail.ac4.yahoo.com> This is the second time that I read that CARBOWAX is used for this procedure. A word of alert: CARBOWAX is an extremely dangerous and carcinogen product. Handle it accordingly! Ren? J. --- On Thu, 9/4/08, Weems, Joyce wrote: From: Weems, Joyce Subject: RE: [Histonet] submitting a cytology specimen for FISH To: "Sharon Campbell" , histonet@lists.utsouthwestern.edu Date: Thursday, September 4, 2008, 12:07 PM Would it be a urine for Uryovision? If so, carbowax is generally supplied by the reference lab that does the testing - or PreservCyt will work if you don't have the kits. It must be added in a certain amount of time. This web site tells about it... http://www.urovysion.com/HealthcareProfessionals_7.aspx And if you are sending to a reference lab, you can get kits and specs from them. Hope this helps, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Sep 4 11:21:40 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Sep 4 11:21:44 2008 Subject: [Histonet] submitting a cytology specimen for FISH In-Reply-To: <62591.60709.qm@web65707.mail.ac4.yahoo.com> References: <982A0A9461F9BF438C7B19A6E425A38358FD2B@ITSSSXM01V6.one.ads.che.org> <62591.60709.qm@web65707.mail.ac4.yahoo.com> Message-ID: <982A0A9461F9BF438C7B19A6E425A38358FD4A@ITSSSXM01V6.one.ads.che.org> Isn't it antifreeze? ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, September 04, 2008 12:17 PM To: Sharon Campbell; histonet@lists.utsouthwestern.edu; Weems, Joyce Subject: RE: [Histonet] submitting a cytology specimen for FISH This is the second time that I read that CARBOWAX is used for this procedure. A word of alert: CARBOWAX is an extremely dangerous and carcinogen product. Handle it accordingly! Ren? J. --- On Thu, 9/4/08, Weems, Joyce wrote: From: Weems, Joyce Subject: RE: [Histonet] submitting a cytology specimen for FISH To: "Sharon Campbell" , histonet@lists.utsouthwestern.edu Date: Thursday, September 4, 2008, 12:07 PM Would it be a urine for Uryovision? If so, carbowax is generally supplied by the reference lab that does the testing - or PreservCyt will work if you don't have the kits. It must be added in a certain amount of time. This web site tells about it... http://www.urovysion.com/HealthcareProfessionals_7.aspx And if you are sending to a reference lab, you can get kits and specs from them. Hope this helps, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From cdemarinis <@t> SARATOGACARE.ORG Thu Sep 4 13:19:19 2008 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis, Carolyn) Date: Thu Sep 4 13:20:25 2008 Subject: [Histonet] ceu's Message-ID: Now that we are NYS licensed histotechnologists we are required to obtain several (12?) CEU credits to maintain licensure. Other than HQUIP, how are techs getting their CEU's without paying a fee? This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. From godsgalnow <@t> aol.com Thu Sep 4 13:40:41 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Sep 4 13:40:58 2008 Subject: [Histonet] FISH Message-ID: <8CADCD4DE14781C-1A88-16D9@FWM-D07.sysops.aol.com> Hello everyone, I have some questions that I would like if you could all answer for me, a survey if you will. ???????????????? Do you do FISH at your facility?? If so what kind (UroVysion, ProVysion, etc)? ???????????????? Who does the preparation (Histotechs, Cytotechs)? ???????????????? Who does the reclassification (Histotechs, Cytotechs, Pathologists)? ?????????????????What kind of volume do you have? ???????????????? Does you state require licensure? ???????????????? What department does FISH belong to (Histology, Cytology, Cytogenetics, Molecular)? ???????????????? Any comments you would like to make? thanks Roxanne From rfields <@t> gidocs.net Thu Sep 4 13:47:55 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Thu Sep 4 13:48:07 2008 Subject: [Histonet] ceu's Message-ID: <07732CE52EC3174AB891DE1C62DB4D8F43EBEF@GIEXCHANGE.gidocs.net> There are several ways to earn CEU's without a fee: I copied this from the ASCP website: 1. Formal continuing education courses: 1 contact hour 1 **ACCME, ASCP CMLE, AACC ACCENT, ASCLS PACE, (50-60 minutes) CE programs sponsored by other professional societies (including state, regional and local societies and chapters), universities and colleges 2. Employer-offered courses (in-service, instrument training, 1 contact hour 1 vendor-sponsored, etc.) (50-60 minutes) 3. College/university coursework 1 quarter hour 10 (science, computer management, education or any other 1 semester hour 15 science related courses) 4. Teleconferences, subscription or online self-instructional 1 contact hour 1 courses for which ACCME, CMLE, ACCENT, PACE or other (50-60 minutes) professional society credits are awarded (see page 5) 5. Completion of advanced ASCP certification or qualification: Specialist/Diplomate certification 25 Categorical or higher level certification 12 Qualification 12 6. Competence Assessment by employer 2 (must use ASCP/BOR Employer Assessment Form) (per year/max 4 pts.) 7. Research & preparation for presenting a workshop each contact hour 3 or course (first time only) of presentation (50-60 minutes) 8. Authoring journal articles for peer-reviewed publications 5 9. Authoring a book (over 300 pages) 21 (under 300 pages) 14 (chapter in a book) 7 10. Editing a book 5 11. Doctoral dissertation 10 12. Master's thesis 4 13. Presenting posters/exhibits 3 14. Serving on examination committees 2 (per year) 15. Serving on committees/boards related to the profession (national, state, regional, local) 2 (per year) 16. Role of on-site inspector for laboratory accreditation (CAP, JCAHO, AABB, COLA, state agency) 1 (per year) or training program accreditation (NAACLS, CAAHEP) Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Demarinis, Carolyn Sent: Thursday, September 04, 2008 1:19 PM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] ceu's Now that we are NYS licensed histotechnologists we are required to obtain several (12?) CEU credits to maintain licensure. Other than HQUIP, how are techs getting their CEU's without paying a fee? This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hana444 <@t> gmail.com Thu Sep 4 14:07:21 2008 From: hana444 <@t> gmail.com (Hana Peter) Date: Thu Sep 4 14:07:29 2008 Subject: [Histonet] Hematoxylin stability In-Reply-To: <48B71065.8020206@gmail.com> References: <48B71065.8020206@gmail.com> Message-ID: <48C031E9.8060808@gmail.com> Hello, thanks to all who answered my question and shared their experiences in hematoxylin making with me. I have decided to make a Lillie-Mayer modification :-) Thanks! Hana Peter From HornHV <@t> archildrens.org Thu Sep 4 15:01:46 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Sep 4 15:01:43 2008 Subject: [Histonet] styrofoam slide mailers Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82DC6@EMAIL.archildrens.org> Several years ago (probably 10) I ordered Styrofoam slide mailers from somewhere. They hold 5-10 slides, are about 3 inches by 4 inches and have a Styrofoam top. I have googled these containers and cannot find anything close to what I want. Does anyone know where I might could find them? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From judi.ford <@t> roche.com Thu Sep 4 16:02:31 2008 From: judi.ford <@t> roche.com (Ford, Judi) Date: Thu Sep 4 16:02:45 2008 Subject: [Histonet] unsubscribe Message-ID: Please take me off the list. I'm heading out on vacation. Thanks, Judi From sharon.osborn <@t> comcast.net Thu Sep 4 16:57:22 2008 From: sharon.osborn <@t> comcast.net (sharon.osborn@comcast.net) Date: Thu Sep 4 16:57:28 2008 Subject: [Histonet] Thom Jannson Message-ID: <090420082157.29647.48C059C2000046A7000073CF2215575114029D010D9C01D202019D0E089C@comcast.net> I am looking for Thom Jannson and if he still has his website up about making TMAs. Please contact me with the website information. I remember the website from a few years ago and ordered some instructional DVDs from it. thanks, sharon osborn IHC QC Lab Vision Fremont, CA From Dorothy.L.Webb <@t> HealthPartners.Com Fri Sep 5 08:04:02 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Sep 5 08:04:11 2008 Subject: [Histonet] Nationals Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635957@hpes1.HealthPartners.int> To all of you who are able to attend Nationals in Pittsburgh, have a GREAT time and learn a lot!! I cannot attend this year due to budget cuts within our hospital system. Anyone learns anything mind shattering or sees any new and exciting piece of equipment, please share!!!!!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From christiegowan <@t> msn.com Fri Sep 5 08:22:28 2008 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Fri Sep 5 08:22:39 2008 Subject: [Histonet] Thom Jannson In-Reply-To: <090420082157.29647.48C059C2000046A7000073CF2215575114029D010D9C01D202019D0E089C@comcast.net> Message-ID: arrayworkshop.com >From: sharon.osborn@comcast.net >To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thom Jannson >Date: Thu, 04 Sep 2008 21:57:22 +0000 > >I am looking for Thom Jannson and if he still has his website up about >making TMAs. Please contact me with the website information. I remember >the website from a few years ago and ordered some instructional DVDs from >it. > >thanks, > >sharon osborn >IHC QC >Lab Vision >Fremont, CA > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Sep 5 09:29:55 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Sep 5 09:30:16 2008 Subject: [Histonet] Nationals In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2705635957@hpes1.HealthPartners.int> References: <0E394B648E5284478A6CCB78E5AFDA2705635957@hpes1.HealthPartners.int> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C156@LTA3VS011.ees.hhs.gov> We'll be thinking of you! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, September 05, 2008 9:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nationals To all of you who are able to attend Nationals in Pittsburgh, have a GREAT time and learn a lot!! I cannot attend this year due to budget cuts within our hospital system. Anyone learns anything mind shattering or sees any new and exciting piece of equipment, please share!!!!!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From njoydobro <@t> aol.com Fri Sep 5 10:06:59 2008 From: njoydobro <@t> aol.com (njoydobro@aol.com) Date: Fri Sep 5 10:07:32 2008 Subject: [Histonet] Antigen Retreival Message-ID: <8CADD802DDA2AC9-111C-10F9@webmail-df01.sysops.aol.com> Does anyone out there have any methods of antigen retreival not using heat? Thanks, Gene From JWeems <@t> sjha.org Fri Sep 5 10:14:01 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Sep 5 10:14:06 2008 Subject: [Histonet] HBME Message-ID: <982A0A9461F9BF438C7B19A6E425A383590116@ITSSSXM01V6.one.ads.che.org> What brand do you all highly recommend? Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From wilson_jm <@t> ciimar.up.pt Fri Sep 5 10:24:53 2008 From: wilson_jm <@t> ciimar.up.pt (Jonathan Wilson) Date: Fri Sep 5 10:25:30 2008 Subject: [Histonet] Antigen Retreival In-Reply-To: <8CADD802DDA2AC9-111C-10F9@webmail-df01.sysops.aol.com> Message-ID: <000401c90f6b$8cc4e100$8edea8c0@cobitis> Gene Try 1%SDS/PBS for 5 min. Rinse well. Works for some antibodies that recognize denatured epitopes. Jon Jonathan Wilson Ecofisiologia-CIIMAR Rua dos Bragas 289 4050-123 Porto Portugal tel +351 22 340 1809 fax + 351 22 339 0608 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of njoydobro@aol.com Sent: Friday, September 05, 2008 4:07 PM To: Histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com Subject: [Histonet] Antigen Retreival Does anyone out there have any methods of antigen retreival not using heat? Thanks, Gene _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Fri Sep 5 10:26:53 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Sep 5 10:26:57 2008 Subject: [Histonet] Antigen Retreival In-Reply-To: <8CADD802DDA2AC9-111C-10F9@webmail-df01.sysops.aol.com> Message-ID: <132532.81363.qm@web53610.mail.re2.yahoo.com> You can try citrate overnight at room temp or there's also enzyme retreival ie: Pro-K, ect. Larry A. Woody Seattle, Wa. --- On Fri, 9/5/08, njoydobro@aol.com wrote: From: njoydobro@aol.com Subject: [Histonet] Antigen Retreival To: Histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com Date: Friday, September 5, 2008, 8:06 AM Does anyone out there have any methods of antigen retreival not using heat? Thanks, Gene _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Sep 5 11:27:06 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Sep 5 11:27:22 2008 Subject: [Histonet] HBME In-Reply-To: <982A0A9461F9BF438C7B19A6E425A383590116@ITSSSXM01V6.one.ads.che.org> Message-ID: <000001c90f74$404a6490$d00f7ca5@lurie.northwestern.edu> We use Dako. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, September 05, 2008 10:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HBME What brand do you all highly recommend? Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jonstonge <@t> hotmail.com Fri Sep 5 11:29:25 2008 From: jonstonge <@t> hotmail.com (Jon St. Onge) Date: Fri Sep 5 11:29:29 2008 Subject: [Histonet] H&E Stainer Message-ID: Good Afternoon and Happy Friday, We are a small research lab looking to purchase a refurbished H&E stainer. Please a little help. What are people using for their routine H&E's? What instruments are work horses and which are lemons to avoid? This instrument would be strictly for H&E's no IHC,Specials, etc. Thank you for any information you provide Refurbished dealer solicitations accepted. Keep smiling it's Friday, Jon "In the end, we will remember not the words of our enemies, but the silence of our friends." --Dr. Martin Luther King, Jr. _________________________________________________________________ Want to do more with Windows Live? Learn ?10 hidden secrets? from Jamie. http://windowslive.com/connect/post/jamiethomson.spaces.live.com-Blog-cns!550F681DAD532637!5295.entry?ocid=TXT_TAGLM_WL_domore_092008 From nancy_schmitt <@t> pa-ucl.com Fri Sep 5 11:32:58 2008 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Fri Sep 5 11:33:05 2008 Subject: [Histonet] amyloid control for congo red Message-ID: <9FC023A4AB52BB4D87DC6456081A822C087D4C@mercury.pa-ucl.com> Happy Friday Histonetters, Can amyloid control slides be cut ahead? Should the block be dipped in paraffin after each use? Having a bit of trouble with this stain - have a new block coming - but want to move forward doing this properly. Thanks for your help Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From rjbuesa <@t> yahoo.com Fri Sep 5 11:36:35 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 5 11:36:39 2008 Subject: [Histonet] Antigen Retreival In-Reply-To: <8CADD802DDA2AC9-111C-10F9@webmail-df01.sysops.aol.com> Message-ID: <15881.75740.qm@web65710.mail.ac4.yahoo.com> You can always "open" the antigenic sites with enzymatic digestions. The antigen retrieval is, by definition, using heat, and sometimes additional pressure to have an even higher heat level. Ren? J. --- On Fri, 9/5/08, njoydobro@aol.com wrote: From: njoydobro@aol.com Subject: [Histonet] Antigen Retreival To: Histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com Date: Friday, September 5, 2008, 11:06 AM Does anyone out there have any methods of antigen retreival not using heat? Thanks, Gene _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From litepath2000 <@t> yahoo.com Fri Sep 5 12:21:11 2008 From: litepath2000 <@t> yahoo.com (NYSHistotech) Date: Fri Sep 5 12:21:16 2008 Subject: [Histonet] CEUs: Histonet Digest, Vol 58, Issue 6 Message-ID: <50694.3084.qm@web58802.mail.re1.yahoo.com> Hi Carolyn and All I just wanted to make sure that everyone is aware that there is NO CEU requirement as part of licensing in New york state. The law (article 165 and the most recent amendment that was passed 6/08) does not contain any specific language regarding a requirement for continuing education.? However, ASCP requires CEU's to maintain certification.? Please check their website for more info. If you have any additional questions, please do not hesitate Best Luis ------- Luis Chiriboga Ph.D. Assistant Professor of Pathology NYU School of Medicine Vice President New York State Histotechnological Society www.nyhisto.org http://tech.groups.yahoo.com/group/NYSHS1972/ Message: 1 Date: Thu, 4 Sep 2008 14:19:19 -0400 From: "Demarinis, Carolyn" Subject: [Histonet] ceu's To: Message-ID: ??? Content-Type: text/plain;??? charset="us-ascii" Now that we are NYS licensed histotechnologists we are required to obtain several (12?) CEU credits to maintain licensure. Other than HQUIP, how are techs getting their CEU's without paying a fee? From brian.chelack <@t> pds.usask.ca Fri Sep 5 12:30:29 2008 From: brian.chelack <@t> pds.usask.ca (Brian Chelack) Date: Fri Sep 5 12:30:36 2008 Subject: [Histonet] RE: Histonet Digest, Vol 58, Issue 6 In-Reply-To: <20080905170822.A1452CD8001@spamf5.usask.ca> References: <20080905170822.A1452CD8001@spamf5.usask.ca> Message-ID: <5BC5B8B1546C49AC8D990B9AE4F5671F@usask.ca> I would beg to differ with Rene on this one. I don't believe antigen retrieval is "by definition" using heat and sometimes pressure. I would define antigen retrieval as any process that restores the original three dimensional structure to a molecule altered by fixation (typically formalin fixation) such that it can be recognized by the detecting antibody. I have performed antigen retrieval by leaving slides overnight in the refrigerator immersed in PBS + 0.05% Tween 20. I'm sure there are many other ways to accomplish antigen retrieval, heat merely accelerates the process. Brian Chelack Special Projects Manager Prairie Diagnostic Services Inc. 52 Campus Drive, Saskatoon, SK S7N 5B4 Phone (306) 966-7211 Fax (306) 966-7244 Email brian.chelack@pds.usask.ca Message: 18 Date: Fri, 5 Sep 2008 09:36:35 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Antigen Retreival To: Histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com, njoydobro@aol.com Message-ID: <15881.75740.qm@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You can always "open" the antigenic sites with enzymatic digestions. The antigen retrieval is, by definition, using heat, and sometimes additional pressure to have an even higher heat level. Reni J. --- On Fri, 9/5/08, njoydobro@aol.com wrote: From: njoydobro@aol.com Subject: [Histonet] Antigen Retreival To: Histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com Date: Friday, September 5, 2008, 11:06 AM Does anyone out there have any methods of antigen retreival not using heat? Thanks, Gene From laurie.colbert <@t> huntingtonhospital.com Fri Sep 5 12:52:36 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 5 12:52:43 2008 Subject: [Histonet] amyloid control for congo red Message-ID: <57BE698966D5C54EAE8612E8941D768303ACCCF7@EXCHANGE3.huntingtonhospital.com> We try not to cut amyloid controls too far in advance - we have had trouble with old slides and poor staining in the past. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, September 05, 2008 9:33 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] amyloid control for congo red Happy Friday Histonetters, Can amyloid control slides be cut ahead? Should the block be dipped in paraffin after each use? Having a bit of trouble with this stain - have a new block coming - but want to move forward doing this properly. Thanks for your help Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathology.histology <@t> gmail.com Fri Sep 5 13:13:00 2008 From: pathology.histology <@t> gmail.com (path lab) Date: Fri Sep 5 13:13:06 2008 Subject: [Histonet] reagent question Message-ID: <73841640809051113w793d111dsd35dde997b64da7@mail.gmail.com> We currently are using a pre made dehydrant (Isopropyl and Methyl alcohol) on our processors and stainer but was wondering about switching to another reagent alcohol (Ethanol, Isopropanol and Methanol). Is there a preference as to what type of alcohol is best for processing routine Histology specimens? Thanks in advance From jqb7 <@t> cdc.gov Fri Sep 5 13:21:05 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Sep 5 13:22:10 2008 Subject: [Histonet] reagent question In-Reply-To: <73841640809051113w793d111dsd35dde997b64da7@mail.gmail.com> References: <73841640809051113w793d111dsd35dde997b64da7@mail.gmail.com> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C160@LTA3VS011.ees.hhs.gov> We use ethanol. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of path lab Sent: Friday, September 05, 2008 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] reagent question We currently are using a pre made dehydrant (Isopropyl and Methyl alcohol) on our processors and stainer but was wondering about switching to another reagent alcohol (Ethanol, Isopropanol and Methanol). Is there a preference as to what type of alcohol is best for processing routine Histology specimens? Thanks in advance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Sep 5 13:32:34 2008 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Sep 5 13:33:21 2008 Subject: [Histonet] amyloid control for congo red References: <57BE698966D5C54EAE8612E8941D768303ACCCF7@EXCHANGE3.huntingtonhospital.com> Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB0028AE7DC@e2k3ms1.urmc-sh.rochester.edu> We would cut them in small batches as well and cut them a little thicker than routine cases. Like at 6 microns. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Colbert Sent: Fri 9/5/2008 1:52 PM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] amyloid control for congo red We try not to cut amyloid controls too far in advance - we have had trouble with old slides and poor staining in the past. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, September 05, 2008 9:33 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] amyloid control for congo red Happy Friday Histonetters, Can amyloid control slides be cut ahead? Should the block be dipped in paraffin after each use? Having a bit of trouble with this stain - have a new block coming - but want to move forward doing this properly. Thanks for your help Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> hitchcock.org Fri Sep 5 13:41:01 2008 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Fri Sep 5 13:41:08 2008 Subject: [Histonet] New cryostat questions Message-ID: I am hoping to purchase a new cryostat within the next few weeks. Currently we have an antiquated Tissue Tek that was purchased in 1988 (!). Right now we have a Leica CM1950 that we are demoing and I am looking at demoing a Thermo Scientific Microm HM550. We really like the Leica (this one has a vacuum and UV disinfection), but of course I haven't tried any other brand to compare. I would appreciate any comments regarding these two cryostats or any others that you feel would merit a demonstration. We do not perform a huge amount of frozen sections, but enough so that we need a unit that is reliable, easy to use, and is ergonomic. Thanks! Lynne Lynne A. Bell, HT (ASCP) Central Vermont Medical Center P. O. Box 547 130 Fisher Road Barre, VT 05641 802-371-4923 From laurie.colbert <@t> huntingtonhospital.com Fri Sep 5 13:41:34 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 5 13:41:41 2008 Subject: [Histonet] reagent question Message-ID: <57BE698966D5C54EAE8612E8941D768303ACCD3C@EXCHANGE3.huntingtonhospital.com> We use reagent alcohol for processing and staining. It is a mixture of isopropyl, ethanol, and methanol. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of path lab Sent: Friday, September 05, 2008 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] reagent question We currently are using a pre made dehydrant (Isopropyl and Methyl alcohol) on our processors and stainer but was wondering about switching to another reagent alcohol (Ethanol, Isopropanol and Methanol). Is there a preference as to what type of alcohol is best for processing routine Histology specimens? Thanks in advance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Fri Sep 5 13:43:11 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Sep 5 13:43:33 2008 Subject: [Histonet] amyloid control for congo red In-Reply-To: <57BE698966D5C54EAE8612E8941D768303ACCCF7@EXCHANGE3.huntingtonhospital.com> Message-ID: Amyloid degrades on cut sections. Slides have to be cut fresh for optimal results. Jackie O' "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/05/2008 12:52 PM To "Nancy Schmitt" , cc Subject RE: [Histonet] amyloid control for congo red We try not to cut amyloid controls too far in advance - we have had trouble with old slides and poor staining in the past. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, September 05, 2008 9:33 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] amyloid control for congo red Happy Friday Histonetters, Can amyloid control slides be cut ahead? Should the block be dipped in paraffin after each use? Having a bit of trouble with this stain - have a new block coming - but want to move forward doing this properly. Thanks for your help Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Fri Sep 5 13:46:10 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Fri Sep 5 13:46:48 2008 Subject: [Histonet] Air purifiers Message-ID: Hello All, Does anyone out there have first hand experience with the air purifiers sold by EMS (Electron Microscopy Sciences)? The web link is pasted below and the pitch (from that site) is also pasted below. I called to see if I could "demo" one but they don't do that. So, obviously I'm reluctant to purchase without having some assurance that they will indeed make a difference in the air quality in my gross tissue dissection room (formalin, xylene, biologic odors, etc.) Are there others in the marketplace that people could recommend? Thanks in advance. Greg http://www.emsdiasum.com/microscopy/products/clean/labair.aspx Lab Air System; Electronic Air Purifiers How do they work ? Electronic Air Purifiers produce a controlled level of Ozone electrically by converting molecules of Oxygen (O2 ) into molecules of Ozone (O3). Ozone, sometimes called activated oxygen, as part of the process of returning to oxygen, casts off its extra atom. That extra atom combines with the molecule of the odor's source and thereby destroys the odor by oxidation. Once Ozone's extra atom is consumed fresh air is left behind which was created by a natural process. Lab-Air Units produce a safe unsaturated level of activated oxygen that neutralizes harmful fumes and eliminates problem odors from organic materials as well as some commonly used chemicals such as: Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone. Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "Success is not the key to happiness. Happiness is the key to success. If you love what you are doing, you will be successful." - Albert Schweitzer ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From themagoos <@t> rushmore.com Fri Sep 5 13:53:16 2008 From: themagoos <@t> rushmore.com (Jason and Heather) Date: Fri Sep 5 13:53:24 2008 Subject: [Histonet] Film Coverslippers Message-ID: <4657.1220640796@rushmore.com> I am wondering if anybody knows of a film coverslipper besides the Tissue-Tek made by Sakura. I know all about this one but I am wondering if there is anything else out there as far as competition. Thank you in advance for your response. Jason McGough HT(ASCP) Acount Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Rapid City, SD 57701 605-343-2267 jmcgough@clinlab.com From mickie25 <@t> netzero.net Fri Sep 5 14:34:48 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Fri Sep 5 14:35:20 2008 Subject: [Histonet] Air purifiers In-Reply-To: References: Message-ID: Greg, Call Rex Johnson (no relation to me) at Creative Waste Solutions in Portland, OR. His number is 503-963-8037, toll free 888-795-8300. He has a very effective charcoal filtered air purifier as well as some organic odor reducer spray which works amazingly well. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Friday, September 05, 2008 11:46 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Air purifiers Hello All, Does anyone out there have first hand experience with the air purifiers sold by EMS (Electron Microscopy Sciences)? The web link is pasted below and the pitch (from that site) is also pasted below. I called to see if I could "demo" one but they don't do that. So, obviously I'm reluctant to purchase without having some assurance that they will indeed make a difference in the air quality in my gross tissue dissection room (formalin, xylene, biologic odors, etc.) Are there others in the marketplace that people could recommend? Thanks in advance. Greg http://www.emsdiasum.com/microscopy/products/clean/labair.aspx Lab Air System; Electronic Air Purifiers How do they work ? Electronic Air Purifiers produce a controlled level of Ozone electrically by converting molecules of Oxygen (O2 ) into molecules of Ozone (O3). Ozone, sometimes called activated oxygen, as part of the process of returning to oxygen, casts off its extra atom. That extra atom combines with the molecule of the odor's source and thereby destroys the odor by oxidation. Once Ozone's extra atom is consumed fresh air is left behind which was created by a natural process. Lab-Air Units produce a safe unsaturated level of activated oxygen that neutralizes harmful fumes and eliminates problem odors from organic materials as well as some commonly used chemicals such as: Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone. Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "Success is not the key to happiness. Happiness is the key to success. If you love what you are doing, you will be successful." - Albert Schweitzer ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.169 / Virus Database: 270.6.16/1654 - Release Date: 9/5/2008 1:24 PM From rjbuesa <@t> yahoo.com Fri Sep 5 14:55:48 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 5 14:55:51 2008 Subject: [Histonet] H&E Stainer In-Reply-To: Message-ID: <287746.10848.qm@web65702.mail.ac4.yahoo.com> Sakura manufactures work horses. Ren? J. --- On Fri, 9/5/08, Jon St. Onge wrote: From: Jon St. Onge Subject: [Histonet] H&E Stainer To: histonet@lists.utsouthwestern.edu Date: Friday, September 5, 2008, 12:29 PM Good Afternoon and Happy Friday, We are a small research lab looking to purchase a refurbished H&E stainer. Please a little help. What are people using for their routine H&E's? What instruments are work horses and which are lemons to avoid? This instrument would be strictly for H&E's no IHC,Specials, etc. Thank you for any information you provide Refurbished dealer solicitations accepted. Keep smiling it's Friday, Jon "In the end, we will remember not the words of our enemies, but the silence of our friends." --Dr. Martin Luther King, Jr. _________________________________________________________________ Want to do more with Windows Live? Learn ?10 hidden secrets? from Jamie. http://windowslive.com/connect/post/jamiethomson.spaces.live.com-Blog-cns!550F681DAD532637!5295.entry?ocid=TXT_TAGLM_WL_domore_092008_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Sep 5 14:59:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 5 14:59:34 2008 Subject: [Histonet] RE: Histonet Digest, Vol 58, Issue 6 In-Reply-To: <5BC5B8B1546C49AC8D990B9AE4F5671F@usask.ca> Message-ID: <127819.23053.qm@web65707.mail.ac4.yahoo.com> By leaving sections in buffer or even water during prolonged periods of time you are just undoing the cross linkage with formalin, if you want to call that antigen retrieval, OK Ren? J. --- On Fri, 9/5/08, Brian Chelack wrote: From: Brian Chelack Subject: [Histonet] RE: Histonet Digest, Vol 58, Issue 6 To: histonet@lists.utsouthwestern.edu Date: Friday, September 5, 2008, 1:30 PM I would beg to differ with Rene on this one. I don't believe antigen retrieval is "by definition" using heat and sometimes pressure. I would define antigen retrieval as any process that restores the original three dimensional structure to a molecule altered by fixation (typically formalin fixation) such that it can be recognized by the detecting antibody. I have performed antigen retrieval by leaving slides overnight in the refrigerator immersed in PBS + 0.05% Tween 20. I'm sure there are many other ways to accomplish antigen retrieval, heat merely accelerates the process. Brian Chelack Special Projects Manager Prairie Diagnostic Services Inc. 52 Campus Drive, Saskatoon, SK S7N 5B4 Phone (306) 966-7211 Fax (306) 966-7244 Email brian.chelack@pds.usask.ca Message: 18 Date: Fri, 5 Sep 2008 09:36:35 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Antigen Retreival To: Histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com, njoydobro@aol.com Message-ID: <15881.75740.qm@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You can always "open" the antigenic sites with enzymatic digestions. The antigen retrieval is, by definition, using heat, and sometimes additional pressure to have an even higher heat level. Reni J. --- On Fri, 9/5/08, njoydobro@aol.com wrote: From: njoydobro@aol.com Subject: [Histonet] Antigen Retreival To: Histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com Date: Friday, September 5, 2008, 11:06 AM Does anyone out there have any methods of antigen retreival not using heat? Thanks, Gene _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Sep 5 15:01:59 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 5 15:02:02 2008 Subject: [Histonet] New cryostat questions In-Reply-To: Message-ID: <574695.4842.qm@web65704.mail.ac4.yahoo.com> The Leica is technologically top of the line. Ren? J. ? --- On Fri, 9/5/08, Bell, Lynne wrote: From: Bell, Lynne Subject: [Histonet] New cryostat questions To: "histonet" Date: Friday, September 5, 2008, 2:41 PM I am hoping to purchase a new cryostat within the next few weeks. Currently we have an antiquated Tissue Tek that was purchased in 1988 (!). Right now we have a Leica CM1950 that we are demoing and I am looking at demoing a Thermo Scientific Microm HM550. We really like the Leica (this one has a vacuum and UV disinfection), but of course I haven't tried any other brand to compare. I would appreciate any comments regarding these two cryostats or any others that you feel would merit a demonstration. We do not perform a huge amount of frozen sections, but enough so that we need a unit that is reliable, easy to use, and is ergonomic. Thanks! Lynne Lynne A. Bell, HT (ASCP) Central Vermont Medical Center P. O. Box 547 130 Fisher Road Barre, VT 05641 802-371-4923 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Sep 5 15:01:45 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Sep 5 15:02:05 2008 Subject: [Histonet] Air purifiers In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E390D664EDE@IBMB7Exchange.digestivespecialists.com> I agree with Mickie. I have been using the unit from Creative Waste Solutions for awhile now and feel it works very well. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Friday, September 05, 2008 3:35 PM To: 'Greg Dobbin'; Histonet@lists.utsouthwestern.edu Cc: Rex Johnson Subject: RE: [Histonet] Air purifiers Greg, Call Rex Johnson (no relation to me) at Creative Waste Solutions in Portland, OR. His number is 503-963-8037, toll free 888-795-8300. He has a very effective charcoal filtered air purifier as well as some organic odor reducer spray which works amazingly well. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Friday, September 05, 2008 11:46 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Air purifiers Hello All, Does anyone out there have first hand experience with the air purifiers sold by EMS (Electron Microscopy Sciences)? The web link is pasted below and the pitch (from that site) is also pasted below. I called to see if I could "demo" one but they don't do that. So, obviously I'm reluctant to purchase without having some assurance that they will indeed make a difference in the air quality in my gross tissue dissection room (formalin, xylene, biologic odors, etc.) Are there others in the marketplace that people could recommend? Thanks in advance. Greg http://www.emsdiasum.com/microscopy/products/clean/labair.aspx Lab Air System; Electronic Air Purifiers How do they work ? Electronic Air Purifiers produce a controlled level of Ozone electrically by converting molecules of Oxygen (O2 ) into molecules of Ozone (O3). Ozone, sometimes called activated oxygen, as part of the process of returning to oxygen, casts off its extra atom. That extra atom combines with the molecule of the odor's source and thereby destroys the odor by oxidation. Once Ozone's extra atom is consumed fresh air is left behind which was created by a natural process. Lab-Air Units produce a safe unsaturated level of activated oxygen that neutralizes harmful fumes and eliminates problem odors from organic materials as well as some commonly used chemicals such as: Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone. Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "Success is not the key to happiness. Happiness is the key to success. If you love what you are doing, you will be successful." - Albert Schweitzer ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.169 / Virus Database: 270.6.16/1654 - Release Date: 9/5/2008 1:24 PM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carlos <@t> clubstaffing.com Fri Sep 5 15:11:08 2008 From: carlos <@t> clubstaffing.com (Carlos Stolk) Date: Fri Sep 5 15:11:13 2008 Subject: [Histonet] Air purifiers In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390D664EDE@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E390D664EDE@IBMB7Exchange.digestivespecialists.com> Message-ID: The air purifiers manufactured by The Sharper Image that have the ozone guard are also a great alternative. Carlos Stolk Account Representative Georgia | New Mexico South Carolina| Texas Phone: 800-875-8999 ext. 258 Fax: 561-367-0884 carlos@clubstaffing.com Club Staffing, Inc. 5901 Broken Sound Pkwy, Ste 500 Boca Raton, FL 33487 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, September 05, 2008 4:02 PM To: 'mickie25@netzero.net'; 'Greg Dobbin'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Air purifiers I agree with Mickie. I have been using the unit from Creative Waste Solutions for awhile now and feel it works very well. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Friday, September 05, 2008 3:35 PM To: 'Greg Dobbin'; Histonet@lists.utsouthwestern.edu Cc: Rex Johnson Subject: RE: [Histonet] Air purifiers Greg, Call Rex Johnson (no relation to me) at Creative Waste Solutions in Portland, OR. His number is 503-963-8037, toll free 888-795-8300. He has a very effective charcoal filtered air purifier as well as some organic odor reducer spray which works amazingly well. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Friday, September 05, 2008 11:46 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Air purifiers Hello All, Does anyone out there have first hand experience with the air purifiers sold by EMS (Electron Microscopy Sciences)? The web link is pasted below and the pitch (from that site) is also pasted below. I called to see if I could "demo" one but they don't do that. So, obviously I'm reluctant to purchase without having some assurance that they will indeed make a difference in the air quality in my gross tissue dissection room (formalin, xylene, biologic odors, etc.) Are there others in the marketplace that people could recommend? Thanks in advance. Greg http://www.emsdiasum.com/microscopy/products/clean/labair.aspx Lab Air System; Electronic Air Purifiers How do they work ? Electronic Air Purifiers produce a controlled level of Ozone electrically by converting molecules of Oxygen (O2 ) into molecules of Ozone (O3). Ozone, sometimes called activated oxygen, as part of the process of returning to oxygen, casts off its extra atom. That extra atom combines with the molecule of the odor's source and thereby destroys the odor by oxidation. Once Ozone's extra atom is consumed fresh air is left behind which was created by a natural process. Lab-Air Units produce a safe unsaturated level of activated oxygen that neutralizes harmful fumes and eliminates problem odors from organic materials as well as some commonly used chemicals such as: Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone. Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "Success is not the key to happiness. Happiness is the key to success. If you love what you are doing, you will be successful." - Albert Schweitzer ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.169 / Virus Database: 270.6.16/1654 - Release Date: 9/5/2008 1:24 PM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Fri Sep 5 15:46:34 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Fri Sep 5 15:47:03 2008 Subject: [Histonet] RE: antigen retrieval In-Reply-To: <127819.23053.qm@web65707.mail.ac4.yahoo.com> References: <5BC5B8B1546C49AC8D990B9AE4F5671F@usask.ca> <127819.23053.qm@web65707.mail.ac4.yahoo.com> Message-ID: Brian Chelack wrote "I would beg to differ with Rene on this one. I don't believe antigen retrieval is "by definition" using heat and sometimes pressure. I would define antigen retrieval as any process that restores the original three dimensional structure to a molecule altered by fixation (typically formalinfixation) such that it can be recognized by the detecting antibody. I have performed antigen retrieval by leaving slides overnight in the refrigerator immersed in PBS + 0.05% Tween 20. I'm sure there are many other ways to accomplish antigen retrieval, heat merely accelerates the process." I think Brian is right, but many don't see it that way, maybe because the heat method has become so prevalent and was the first to be called "antigen Retrieval." Before that we simply used digestion, or "enzyme treatment" and called it that. However, in their book "Antigen Retrieval Techniques," Shan-Rong Shi et al, the inventors of heat antigen retrieval, BEGGED people to use the term "antigen retrieval" within ANY description, for ANY method that improves the antigen/antibody reaction by pretreating the tissue - enzyme, heat, non-heat, chemical methods, whatever. Their reasoning was that 1) all these methods "retrieve" antigen reaction with antibodies and 2) there has been such an explosion of terms used to describe the process of enhancing antigen/antibody reaction that it has become impossible to search out all the articles that use such methods. A standard term, even if others are added to more fully describe the process, will allow all of us to keep up with the literature without trying to guess what the next person may call the method they use. Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology ThermoFisher Scientific From Maxim_71 <@t> mail.ru Sat Sep 6 14:38:42 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Sat Sep 6 15:34:33 2008 Subject: [Histonet] reagent question Message-ID: <03082354.20080906233842@mail.ru> More than one year we uses isopropanol as dehydratant and enjoy for quality of specimens, which processed with this reagent. It is very useful for bone, cartillage, uterus, breast, colon, skin, eyeball and other difficult specimens. Some unpleasant odor of "cat urine" does not disturb to use it in manual processing. We believes, that will be time, when we will have a vacuum processor... For slides we use acetone as H&E as SS. We stain all our slides manually. Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From SHargrove <@t> urhcs.org Sun Sep 7 17:25:51 2008 From: SHargrove <@t> urhcs.org (SHargrove@urhcs.org) Date: Sun Sep 7 17:26:10 2008 Subject: [Histonet] Susie Hargrove is out of the office. Message-ID: I will be out of the office starting 09/07/2008 and will not return until 09/11/2008. I will respond to your message when I return. If immediate assistance is needed please call 3198. From Stephen.Eyres <@t> sanofi-aventis.com Mon Sep 8 09:09:04 2008 From: Stephen.Eyres <@t> sanofi-aventis.com (Stephen.Eyres@sanofi-aventis.com) Date: Mon Sep 8 09:09:15 2008 Subject: [Histonet] Information on the use of the Medite USE 33 ultrasonic decalcifier. Message-ID: <90B6684A9D6DAF468F7A5DC148754E1DC21B39@ALPW31.f2.enterprise> Hi, In an effort to speed up our decalcification process, we purchased a USE 33 ultrasonic decalcifier. However, results were variable and the UK agent disappeared off the face of the earth, whilst Medite failed to respond also. I'd be interested in the experiences of other users of the system. Cheers Steve From NMargaryan <@t> childrensmemorial.org Mon Sep 8 10:39:00 2008 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Sep 8 10:40:07 2008 Subject: [Histonet] Air purifiers Message-ID: Hi histonetters, Is there any Air purifiers for DAB? I guess I am allergic to that reagent; I can test it after using. I am OK to Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone because I use them in the hood, but DAB is a step in autostainer. Regards, Naira From dellav <@t> musc.edu Mon Sep 8 11:15:42 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Mon Sep 8 11:15:46 2008 Subject: [Histonet] Air purifiers In-Reply-To: References: Message-ID: Greg, I looked into an ozone generator for odor control in the morgue. At the time, I discovered that the safety of ozone generators is apparently controversial but I don't recall the details. Apparently the Feds consider ozone hazardous if my memory is correct on this. Would be worth an internet search at the least if you are considering going this route. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Friday, September 05, 2008 2:46 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Air purifiers Hello All, Does anyone out there have first hand experience with the air purifiers sold by EMS (Electron Microscopy Sciences)? The web link is pasted below and the pitch (from that site) is also pasted below. I called to see if I could "demo" one but they don't do that. So, obviously I'm reluctant to purchase without having some assurance that they will indeed make a difference in the air quality in my gross tissue dissection room (formalin, xylene, biologic odors, etc.) Are there others in the marketplace that people could recommend? Thanks in advance. Greg http://www.emsdiasum.com/microscopy/products/clean/labair.aspx Lab Air System; Electronic Air Purifiers How do they work ? Electronic Air Purifiers produce a controlled level of Ozone electrically by converting molecules of Oxygen (O2 ) into molecules of Ozone (O3). Ozone, sometimes called activated oxygen, as part of the process of returning to oxygen, casts off its extra atom. That extra atom combines with the molecule of the odor's source and thereby destroys the odor by oxidation. Once Ozone's extra atom is consumed fresh air is left behind which was created by a natural process. Lab-Air Units produce a safe unsaturated level of activated oxygen that neutralizes harmful fumes and eliminates problem odors from organic materials as well as some commonly used chemicals such as: Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone. Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "Success is not the key to happiness. Happiness is the key to success. If you love what you are doing, you will be successful." - Albert Schweitzer ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Sep 8 11:47:21 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 8 11:47:26 2008 Subject: [Histonet] Air purifiers In-Reply-To: Message-ID: <387811.82365.qm@web65706.mail.ac4.yahoo.com> If you can taste DAB after working with it (either weighing of diluting it) it means that you need to wear an AIR?MASK, the air purifier will not be enough. Remember that DAB is cancerigenous. Ren? J. --- On Mon, 9/8/08, Margaryan, Naira wrote: From: Margaryan, Naira Subject: [Histonet] Air purifiers To: histonet@lists.utsouthwestern.edu Cc: "mickie25@netzero.net" <'mickie25@netzero.net'>, "Rex Johnson" Date: Monday, September 8, 2008, 11:39 AM Hi histonetters, Is there any Air purifiers for DAB? I guess I am allergic to that reagent; I can test it after using. I am OK to Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone because I use them in the hood, but DAB is a step in autostainer. Regards, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Sep 8 11:48:57 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 8 11:49:00 2008 Subject: Fw: Re: [Histonet] Air purifiers Message-ID: <278910.12957.qm@web65713.mail.ac4.yahoo.com> --- On Mon, 9/8/08, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] Air purifiers To: histonet@lists.utsouthwestern.edu, Date: Monday, September 8, 2008, 12:47 PM If you can taste DAB after working with it (either weighing of diluting it) it means that you need to wear an AIR?MASK, the air purifier will not be enough. Remember that DAB is cancerigenous. Ren? J. --- On Mon, 9/8/08, Margaryan, Naira wrote: From: Margaryan, Naira Subject: [Histonet] Air purifiers To: histonet@lists.utsouthwestern.edu Cc: "mickie25@netzero.net" <'mickie25@netzero.net'>, "Rex Johnson" Date: Monday, September 8, 2008, 11:39 AM Hi histonetters, Is there any Air purifiers for DAB? I guess I am allergic to that reagent; I can test it after using. I am OK to Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone because I use them in the hood, but DAB is a step in autostainer. Regards, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sarah.Hale <@t> uvm.edu Mon Sep 8 12:36:38 2008 From: Sarah.Hale <@t> uvm.edu (Hale, Sarah) Date: Mon Sep 8 12:36:42 2008 Subject: [Histonet] VEGFR1 and 2 Message-ID: <36830148D2AC3E48A318C1436C89462B088BED78@MED05.med.uvm> Hi! Does anyone have any experience with IHC for VEGFR1 and 2 in rat arteries? Our lab has had trouble getting good staining especially with paraffin sections. Thanks! -Sarah __________________________________________________ Sarah Hale, Ph.D. Postdoctoral Associate Dept. of OB/Gyn and Reproductive Sciences C215 Given Bldg. University of Vermont Burlington, VT 05405 ph: 802.656.1207 email: sarah.hale@uvm.edu From nsnwl <@t> neuro.hfh.edu Mon Sep 8 12:53:16 2008 From: nsnwl <@t> neuro.hfh.edu (Nancy Lemke) Date: Mon Sep 8 12:53:26 2008 Subject: [Histonet] VEGFR1 and 2 Message-ID: Hi Sarah, I can heartily recommend Santa Cruz sc-6251. FFPE: retrieve in citrate buffer, pH 6.0- boil for 10 minutes, cool for 20. Block, then incubate in sc-6251 at 1:1500 for 30 minutes at room temp. I use Biocare 4+ detection as given on data sheet and Biocare Betazoid DAB for 4 minutes and get beautiful, clean results. I use this in rat sections. Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Hale, Sarah" Sarah.Hale@uvm.edu Date: Mon, 08 Sep 2008 13:37:43 -0400 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VEGFR1 and 2 > Hi! > > Does anyone have any experience with IHC for VEGFR1 and 2 in rat > arteries? Our lab has had trouble getting good staining especially with > paraffin sections. > > Thanks! > -Sarah > > __________________________________________________ > Sarah Hale, Ph.D. > Postdoctoral Associate > Dept. of OB/Gyn and Reproductive Sciences > C215 Given Bldg. > University of Vermont > Burlington, VT 05405 > ph: 802.656.1207 > email: sarah.hale@uvm.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From Sharon.Davis-Devine <@t> carle.com Mon Sep 8 16:15:05 2008 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Mon Sep 8 16:15:10 2008 Subject: [Histonet] Using Ventana with new antibody lots Message-ID: <44780C571F28624DBB446DE55C4D733A021E0A94@EXCHANGEBE1.carle.com> Ok, Histonetters I have another question for you. According the CAP checklist question: ANP.22750 Phase II Has the laboratory documented evaluation of new antibody lots and new antibodies, prior to use in patient diagnosis? NOTE: For newly introduced antibodies, staining conditions should be evaluated in cases expected to be positive and negative for the antigen of interest. Ideally, a series of sufficient size should be run to give the laboratory an idea of the sensitivity and specificity of the test. How are you folks handling this question when using Ventana? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com From AnthonyH <@t> chw.edu.au Mon Sep 8 17:49:07 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Sep 8 17:49:24 2008 Subject: [Histonet] Air purifiers In-Reply-To: Message-ID: Are you sure that it not the hydrogen peroxide that you may be allergic to. The concentrated (30%) form will bleach your fingers if you are not wearing gloves (or if there is a pinhole in them!) making them quite itchy. I am not aware of any cases of DAB allergy, though you may be the first or, most probably I need to research this more. Is there anyone else who might have a DAB allergy? We probably need to have our OH&S committee look into it. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Tuesday, 9 September 2008 1:39 AM To: histonet@lists.utsouthwestern.edu Cc: mickie25@netzero.net; Rex Johnson Subject: [Histonet] Air purifiers Hi histonetters, Is there any Air purifiers for DAB? I guess I am allergic to that reagent; I can test it after using. I am OK to Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone because I use them in the hood, but DAB is a step in autostainer. Regards, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Mon Sep 8 18:43:52 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Sep 8 18:44:05 2008 Subject: [Histonet] Air purifiers In-Reply-To: <387811.82365.qm@web65706.mail.ac4.yahoo.com> Message-ID: Ozone may indeed be of some concern. Some research notes: "High levels of atmospheric ozone in Florence air correlated with DNA damage, and to the prevalence of inflammatory pathologies of the upper respiratory tract, although the ozone concentrations were below the Italian recommended attention level. Furthermore, higher levels of DNA damage were correlated with a dysfunction in the ability to maintain a normal epithelial cell structure. These data suggest an association between ozone air levels and damage in the upper respiratory tract. It remains unclear whether ozone itself or other associated pollutants are responsible for the observed alterations." (Environ. Mol. Mutagen. 42:127-135, 2003) "Beagle dogs exposed for 8 hours a day to 3 ppm O3 for 18 months showed cytologic changes that indicate metabolic alterations. The endoplasmic reticulum of the type 2 alveolar epithelial cells in the proximal alveoli were frequently dilated and contained a moderately electron-dense substance having a periodicity of approximately 754 A. This condition was accompanied by a substantial reduction in the lamellar membranes in the characteristic lamellar bodies of the type 2 cells, suggesting a sequestering of protein in the endoplasmic reticulum. In addition, endothelial cells contained paracrystalline arrays of cytoplasmic membranes not observed in control animals" (Am J Pathol. 1973 December; 73(3): 711-726) "The interaction of ozone with some molecules results in an increased production of free radicals. The objective of this study was to identify whether acute ozone exposure to 1-1.5 ppm for 4 h, produced cytological and ultrastructural modifications in the olfactory bulb cells. The results showed that in rats exposed to ozone there was a significant loss of dendritic spines on primary and secondary dendrites of granule cells, whereas the control rats did not present such changes. Besides these exposed cells showed vacuolation of neuronal cytoplasm, swelling of Golgi apparatus and mitochondrion, dilation cisterns of the rough endoplasmic reticulum. These findings suggest that oxidative stress produced by ozone induces alterations in the granule layer of the olfactory bulb, which may be related to functional modifications" (Neuroscience Letters Volume 274, Issue 1, 15 October 1999, Pages 1-4) "Inhalation of the pulmonary irritant ozone is associated with an accumulation of macrophages in the lung. These cells, along with type II epithelial cells, are activated to release increased quantities of hydrogen peroxide and nitric oxide, two reactive mediators that have been implicated in tissue injury" (Am J Respir Cell Mol Biol. 1996 Jun ;14 (6):516-25). "ozone-induced high-protein alveolar edema is pathogenetically linked to pulmonary hyperemia, deficiency of surfactant tubular myelin, and associated lung dysfunctions" (Toxicol Appl Pharmacol Vol. 117 Issue 1 Pg. 37-45 Nov 1992). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, 9 September 2008 2:47 AM To: histonet@lists.utsouthwestern.edu; Margaryan, Naira Cc: Rex Johnson; mickie25@netzero.net Subject: Re: [Histonet] Air purifiers If you can taste DAB after working with it (either weighing of diluting it) it means that you need to wear an AIR?MASK, the air purifier will not be enough. Remember that DAB is cancerigenous. Ren? J. --- On Mon, 9/8/08, Margaryan, Naira wrote: From: Margaryan, Naira Subject: [Histonet] Air purifiers To: histonet@lists.utsouthwestern.edu Cc: "mickie25@netzero.net" <'mickie25@netzero.net'>, "Rex Johnson" Date: Monday, September 8, 2008, 11:39 AM Hi histonetters, Is there any Air purifiers for DAB? I guess I am allergic to that reagent; I can test it after using. I am OK to Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone because I use them in the hood, but DAB is a step in autostainer. Regards, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From blebl2 <@t> lsuhsc.edu Tue Sep 9 06:03:09 2008 From: blebl2 <@t> lsuhsc.edu (Leblanc, Barbara Ann) Date: Tue Sep 9 06:04:01 2008 Subject: [Histonet] Varistain Gemini Auto Stainer Message-ID: Hi everyone: We have a Varistain Gemini ES autostainer. We are having variability in the stain quality of the slides. Some slides are stained properly while others are stained lighter. This is sometimes on the same slide (top section stained good; bottom section not good); sometimes some slides in the rack will stain okay and others in the same rack will not. Does anyone have this stainer and has experienced this problem? Thank you, Barbara From lblazek <@t> digestivespecialists.com Tue Sep 9 06:21:15 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Sep 9 06:21:16 2008 Subject: [Histonet] RE: Varistain Gemini Auto Stainer In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39119A05C3@IBMB7Exchange.digestivespecialists.com> Barbara, I don't have that stainer but have had that problem from time to time. If you are using recycled alcohol in your last alcohol station or if your alcohol is not really at 100% that may be the problem. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leblanc, Barbara Ann Sent: Tuesday, September 09, 2008 7:03 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Varistain Gemini Auto Stainer Hi everyone: We have a Varistain Gemini ES autostainer. We are having variability in the stain quality of the slides. Some slides are stained properly while others are stained lighter. This is sometimes on the same slide (top section stained good; bottom section not good); sometimes some slides in the rack will stain okay and others in the same rack will not. Does anyone have this stainer and has experienced this problem? Thank you, Barbara _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Sep 9 07:25:22 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 9 07:25:25 2008 Subject: [Histonet] Using Ventana with new antibody lots In-Reply-To: <44780C571F28624DBB446DE55C4D733A021E0A94@EXCHANGEBE1.carle.com> Message-ID: <397080.62015.qm@web65709.mail.ac4.yahoo.com> This applies to ALL new lots or antibodies, not just for Ventana. 1- when I received a new lot, I tested it WITHOUT? a patient section (just with a + control) before using it with patients. The results were documented in the Abs log. 2- the other question refers to the introduction of a new Ab, something you had not used before. In that case you have to run it with a series of potentially + controls, at different dilution rates to determine the working concentration and the suitability for the purpose it is intended for. It is like a validation test and should be documented also. The pathologist has to sign the validation and concentration tests. Ren? J. --- On Mon, 9/8/08, Sharon.Davis-Devine wrote: From: Sharon.Davis-Devine Subject: [Histonet] Using Ventana with new antibody lots To: histonet@lists.utsouthwestern.edu Date: Monday, September 8, 2008, 5:15 PM Ok, Histonetters I have another question for you. According the CAP checklist question: ANP.22750 Phase II Has the laboratory documented evaluation of new antibody lots and new antibodies, prior to use in patient diagnosis? NOTE: For newly introduced antibodies, staining conditions should be evaluated in cases expected to be positive and negative for the antigen of interest. Ideally, a series of sufficient size should be run to give the laboratory an idea of the sensitivity and specificity of the test. How are you folks handling this question when using Ventana? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Sep 9 07:43:14 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 9 07:43:18 2008 Subject: [Histonet] Varistain Gemini Auto Stainer In-Reply-To: Message-ID: <613411.20328.qm@web65707.mail.ac4.yahoo.com> Confronted with an instrument problem that I could not solve by myself, the best option always is to contact the instrument vendor. They usually are able to help. I advise you to do the same. Ren? J. --- On Tue, 9/9/08, Leblanc, Barbara Ann wrote: From: Leblanc, Barbara Ann Subject: [Histonet] Varistain Gemini Auto Stainer To: histonet@pathology.swmed.edu Date: Tuesday, September 9, 2008, 7:03 AM Hi everyone: We have a Varistain Gemini ES autostainer. We are having variability in the stain quality of the slides. Some slides are stained properly while others are stained lighter. This is sometimes on the same slide (top section stained good; bottom section not good); sometimes some slides in the rack will stain okay and others in the same rack will not. Does anyone have this stainer and has experienced this problem? Thank you, Barbara _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gdeville <@t> deltapathology.com Tue Sep 9 08:46:19 2008 From: gdeville <@t> deltapathology.com (Gwen Deville) Date: Tue Sep 9 08:49:57 2008 Subject: [Histonet] Using Ventana with new antibody lots In-Reply-To: <397080.62015.qm@web65709.mail.ac4.yahoo.com> References: <44780C571F28624DBB446DE55C4D733A021E0A94@EXCHANGEBE1.carle.com> <397080.62015.qm@web65709.mail.ac4.yahoo.com> Message-ID: <003901c91282$708d59d0$51a80d70$@com> I recently asked CAP for specific details of how antibody validation should be handled since there are many different opinions out there, from lab to lab, tech to tech, and CAP and CLIA. Especially with CLIA enforcing more regs on the anatomic pathology lab. In the past, we in AP did not feel like many of these regs fit in our department, most seemed to be more geared to the clinical laboratory. After experiencing a CLIA visit, I can tell you we have changed our thinking. Dr. Patrick Fitzgibbons responded to my question. There is a book that CAP publishes that every laboratory should have called, "QUALITY MANAGEMENT IN ANATOMIC PATHOLOGY". Chapter 8 deals specifically with IHC and Antibody validation. It details to what extent they should be tested prior to patient testing. Who would know better what is expected in this area? Gwen Deville, Histology Supv. Delta Pathology, Mid-Louisiana Box 30113, 211 Fourth Street Alexandria, LA 71301 ? Work: (318)473-3943 / (318) 473-3180 Pager: (318) 427-5444 Email: gdeville@deltapathology.com ? NOTICE:? This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient.? If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissenmination, distribution, forwarding, printing, or copying of the email or any attached files is strictly prohibited.? If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, September 09, 2008 7:25 AM To: histonet@lists.utsouthwestern.edu; Sharon.Davis-Devine Subject: Re: [Histonet] Using Ventana with new antibody lots This applies to ALL new lots or antibodies, not just for Ventana. 1- when I received a new lot, I tested it WITHOUT? a patient section (just with a + control) before using it with patients. The results were documented in the Abs log. 2- the other question refers to the introduction of a new Ab, something you had not used before. In that case you have to run it with a series of potentially + controls, at different dilution rates to determine the working concentration and the suitability for the purpose it is intended for. It is like a validation test and should be documented also. The pathologist has to sign the validation and concentration tests. Ren? J. --- On Mon, 9/8/08, Sharon.Davis-Devine wrote: From: Sharon.Davis-Devine Subject: [Histonet] Using Ventana with new antibody lots To: histonet@lists.utsouthwestern.edu Date: Monday, September 8, 2008, 5:15 PM Ok, Histonetters I have another question for you. According the CAP checklist question: ANP.22750 Phase II Has the laboratory documented evaluation of new antibody lots and new antibodies, prior to use in patient diagnosis? NOTE: For newly introduced antibodies, staining conditions should be evaluated in cases expected to be positive and negative for the antigen of interest. Ideally, a series of sufficient size should be run to give the laboratory an idea of the sensitivity and specificity of the test. How are you folks handling this question when using Ventana? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Tue Sep 9 09:07:20 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Sep 9 09:07:31 2008 Subject: [Histonet] Formaldehyde and scrubs Message-ID: <57BE698966D5C54EAE8612E8941D768303ACD1BE@EXCHANGE3.huntingtonhospital.com> Does anyone know of any regulations (OSHA or otherwise) that require employers to provide scrubs to anyone who works with formaldehyde (in order to prevent them from taking contaminated clothing home)? Laurie Colbert From rjbuesa <@t> yahoo.com Tue Sep 9 09:28:48 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 9 09:28:52 2008 Subject: [Histonet] Formaldehyde and scrubs In-Reply-To: <57BE698966D5C54EAE8612E8941D768303ACD1BE@EXCHANGE3.huntingtonhospital.com> Message-ID: <422996.27861.qm@web65710.mail.ac4.yahoo.com> That is a general OSHA regulation. Exposed clothes to any product or hazardous environment should not be taken home. Ren? J. --- On Tue, 9/9/08, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Formaldehyde and scrubs To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 9, 2008, 10:07 AM Does anyone know of any regulations (OSHA or otherwise) that require employers to provide scrubs to anyone who works with formaldehyde (in order to prevent them from taking contaminated clothing home)? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Tue Sep 9 09:38:39 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Sep 9 09:39:17 2008 Subject: [Histonet] Formaldehyde and scrubs In-Reply-To: <57BE698966D5C54EAE8612E8941D768303ACD1BE@EXCHANGE3.huntingtonhospital.com> Message-ID: We just have fluid resistant labcoats. My daughter is an emergency room tech, and she has to purchase her own scrubs for work, which she wears home, and washes them herself. That's kinda strange to me. Cheap hospital, I guess. "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/09/2008 09:07 AM To cc Subject [Histonet] Formaldehyde and scrubs Does anyone know of any regulations (OSHA or otherwise) that require employers to provide scrubs to anyone who works with formaldehyde (in order to prevent them from taking contaminated clothing home)? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Sep 9 10:33:36 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Sep 9 10:33:46 2008 Subject: [Histonet] Formaldehyde and scrubs In-Reply-To: <57BE698966D5C54EAE8612E8941D768303ACD1BE@EXCHANGE3.huntingtonhospital.com> Message-ID: As far as I can remember, OHSA requires that employees wear a fluid impervious lab coat to protect their clothing and it is the responsibility of the employer to provide and launder the lab coats. Jennifer "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/09/2008 07:10 AM To cc Subject [Histonet] Formaldehyde and scrubs Does anyone know of any regulations (OSHA or otherwise) that require employers to provide scrubs to anyone who works with formaldehyde (in order to prevent them from taking contaminated clothing home)? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Sep 9 10:42:02 2008 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Sep 9 10:43:16 2008 Subject: [Histonet] Formaldehyde and scrubs References: Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB0028AE7E8@e2k3ms1.urmc-sh.rochester.edu> I believe that the employer can also provide disposable lab aprons that do the same, not just a lab coat. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jennifer MacDonald Sent: Tue 9/9/2008 11:33 AM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Formaldehyde and scrubs As far as I can remember, OHSA requires that employees wear a fluid impervious lab coat to protect their clothing and it is the responsibility of the employer to provide and launder the lab coats. Jennifer "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/09/2008 07:10 AM To cc Subject [Histonet] Formaldehyde and scrubs Does anyone know of any regulations (OSHA or otherwise) that require employers to provide scrubs to anyone who works with formaldehyde (in order to prevent them from taking contaminated clothing home)? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From YinongChen <@t> texashealth.org Tue Sep 9 12:23:25 2008 From: YinongChen <@t> texashealth.org (Chen, Yinong) Date: Tue Sep 9 12:23:31 2008 Subject: [Histonet] HISTOTECHNICIAN/HISTOTECHNOLOGIS-Dallas,Texas Message-ID: HISTOTECHNICIAN/HISTOTECHNOLOGIST Presbyterian Hospital of Dallas,is currently seeking a registered or eligible candidate for first shift full-time employment. Responsibilities include embedding,microtomy,special stains,frozen sections,and gross dictation assistance. Excellent benefits,including:onsite child care, hospital wide bonus eligibility, matching 401K, competitive salaries, tuition reimbursement, annual merit increase, and employee referral bonus. Interested candidates please contact: maureenhoops@texashealth.org or apply on line at http://www.texashealth.org The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From YinongChen <@t> texashealth.org Tue Sep 9 12:23:25 2008 From: YinongChen <@t> texashealth.org (Chen, Yinong) Date: Tue Sep 9 12:23:34 2008 Subject: [Histonet] HISTOTECHNICIAN/HISTOTECHNOLOGIS-Dallas,Texas Message-ID: HISTOTECHNICIAN/HISTOTECHNOLOGIST Presbyterian Hospital of Dallas,is currently seeking a registered or eligible candidate for first shift full-time employment. Responsibilities include embedding,microtomy,special stains,frozen sections,and gross dictation assistance. Excellent benefits,including:onsite child care, hospital wide bonus eligibility, matching 401K, competitive salaries, tuition reimbursement, annual merit increase, and employee referral bonus. Interested candidates please contact: maureenhoops@texashealth.org or apply on line at http://www.texashealth.org The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From kenneth.a.troutman <@t> Vanderbilt.Edu Tue Sep 9 13:05:17 2008 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Tue Sep 9 13:05:24 2008 Subject: [Histonet] Varistain Gemini Auto Stainer Message-ID: <37DEF9AF72994947AF693956A59B9B660127FFA3@mailbe03.mc.vanderbilt.edu> There are a few things that could be wrong: 1) Recycled reagents (which I believe someone mentioned) 2) If you are using the heaters on the stainer, one of them may be malfunctioning. If you are using a hot plate, make sure all of your slides are completely dry before staining. 3) Check the level of all reagents (to make sure the entire slide is immersed in solution. This may not be the case since you are seeing better staining on the top of the slide.) 4) I have also seen this where it is microtomist dependent. I would definitely call the vendor. My money is on the heater :), but a qualified engineer should look at it. Good luck, Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN Message: 6 Date: Tue, 9 Sep 2008 06:03:09 -0500 From: "Leblanc, Barbara Ann" Subject: [Histonet] Varistain Gemini Auto Stainer To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi everyone: We have a Varistain Gemini ES autostainer. We are having variability in the stain quality of the slides. Some slides are stained properly while others are stained lighter. This is sometimes on the same slide (top section stained good; bottom section not good); sometimes some slides in the rack will stain okay and others in the same rack will not. Does anyone have this stainer and has experienced this problem? Thank you, Barbara From YinongChen <@t> texashealth.org Tue Sep 9 13:14:05 2008 From: YinongChen <@t> texashealth.org (Chen, Yinong) Date: Tue Sep 9 13:14:10 2008 Subject: [Histonet] Dallas, Texas - HISTOTECHNICIAN / HISTOTECHNOLOGIST job opening Message-ID: HISTOTECHNICIAN / HISTOTECHNOLOGIST Presbyterian Hospital of Dallas,is currently seeking a registered or eligible candidate for first shift full-time employment. Responsibilities include embedding, microtomy, special stains, frozen sections, and gross dictation assistance. Excellent benefits, including: onsite child care, hospital wide bonus eligibility, matching 401K, competitive salaries, tuition reimbursement, annual merit increase, and employee referral bonus. Interested candidates please contact: maureenhoops@texashealth.org or apply on line at http://www.texashealth.org The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From YinongChen <@t> texashealth.org Tue Sep 9 13:14:05 2008 From: YinongChen <@t> texashealth.org (Chen, Yinong) Date: Tue Sep 9 13:14:13 2008 Subject: [Histonet] Dallas, Texas - HISTOTECHNICIAN / HISTOTECHNOLOGIST job opening Message-ID: HISTOTECHNICIAN / HISTOTECHNOLOGIST Presbyterian Hospital of Dallas,is currently seeking a registered or eligible candidate for first shift full-time employment. Responsibilities include embedding, microtomy, special stains, frozen sections, and gross dictation assistance. Excellent benefits, including: onsite child care, hospital wide bonus eligibility, matching 401K, competitive salaries, tuition reimbursement, annual merit increase, and employee referral bonus. Interested candidates please contact: maureenhoops@texashealth.org or apply on line at http://www.texashealth.org The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From MadaryJ <@t> MedImmune.com Tue Sep 9 13:41:11 2008 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Tue Sep 9 13:41:31 2008 Subject: [Histonet] Formaldehyde and scrubs In-Reply-To: Message-ID: What I like is when people who wear scrubs walk the hallways and even eat in the cafeteria with blood, tissue, and smelling of chemicals on their scrubs. I really like that a lot. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory Mgr One Medimmune Way, Lab 2438-Area 4 Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, September 09, 2008 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 58, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. VEGFR1 and 2 (Hale, Sarah) 2. Re: VEGFR1 and 2 (Nancy Lemke) 3. Using Ventana with new antibody lots (Sharon.Davis-Devine) 4. RE: Air purifiers (Tony Henwood) 5. RE: Air purifiers (Tony Henwood) 6. Varistain Gemini Auto Stainer (Leblanc, Barbara Ann) 7. RE: Varistain Gemini Auto Stainer (Blazek, Linda) 8. Re: Using Ventana with new antibody lots (Rene J Buesa) 9. Re: Varistain Gemini Auto Stainer (Rene J Buesa) 10. RE: Using Ventana with new antibody lots (Gwen Deville) 11. Formaldehyde and scrubs (Laurie Colbert) 12. Re: Formaldehyde and scrubs (Rene J Buesa) 13. Re: Formaldehyde and scrubs (Jackie M O'Connor) 14. Re: Formaldehyde and scrubs (Jennifer MacDonald) 15. RE: Formaldehyde and scrubs (McMahon, Loralee A) ---------------------------------------------------------------------- Message: 1 Date: Mon, 8 Sep 2008 13:36:38 -0400 From: "Hale, Sarah" Subject: [Histonet] VEGFR1 and 2 To: Message-ID: <36830148D2AC3E48A318C1436C89462B088BED78@MED05.med.uvm> Content-Type: text/plain; charset="US-ASCII" Hi! Does anyone have any experience with IHC for VEGFR1 and 2 in rat arteries? Our lab has had trouble getting good staining especially with paraffin sections. Thanks! -Sarah __________________________________________________ Sarah Hale, Ph.D. Postdoctoral Associate Dept. of OB/Gyn and Reproductive Sciences C215 Given Bldg. University of Vermont Burlington, VT 05405 ph: 802.656.1207 email: sarah.hale@uvm.edu ------------------------------ Message: 2 Date: Mon, 08 Sep 2008 13:53:16 -0400 From: "Nancy Lemke" Subject: Re: [Histonet] VEGFR1 and 2 To: "Hale, Sarah" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hi Sarah, I can heartily recommend Santa Cruz sc-6251. FFPE: retrieve in citrate buffer, pH 6.0- boil for 10 minutes, cool for 20. Block, then incubate in sc-6251 at 1:1500 for 30 minutes at room temp. I use Biocare 4+ detection as given on data sheet and Biocare Betazoid DAB for 4 minutes and get beautiful, clean results. I use this in rat sections. Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Hale, Sarah" Sarah.Hale@uvm.edu Date: Mon, 08 Sep 2008 13:37:43 -0400 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VEGFR1 and 2 > Hi! > > Does anyone have any experience with IHC for VEGFR1 and 2 in rat > arteries? Our lab has had trouble getting good staining especially > with paraffin sections. > > Thanks! > -Sarah > > __________________________________________________ > Sarah Hale, Ph.D. > Postdoctoral Associate > Dept. of OB/Gyn and Reproductive Sciences > C215 Given Bldg. > University of Vermont > Burlington, VT 05405 > ph: 802.656.1207 > email: sarah.hale@uvm.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== ------------------------------ Message: 3 Date: Mon, 8 Sep 2008 16:15:05 -0500 From: "Sharon.Davis-Devine" Subject: [Histonet] Using Ventana with new antibody lots To: Message-ID: <44780C571F28624DBB446DE55C4D733A021E0A94@EXCHANGEBE1.carle.com> Content-Type: text/plain; charset="us-ascii" Ok, Histonetters I have another question for you. According the CAP checklist question: ANP.22750 Phase II Has the laboratory documented evaluation of new antibody lots and new antibodies, prior to use in patient diagnosis? NOTE: For newly introduced antibodies, staining conditions should be evaluated in cases expected to be positive and negative for the antigen of interest. Ideally, a series of sufficient size should be run to give the laboratory an idea of the sensitivity and specificity of the test. How are you folks handling this question when using Ventana? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com ------------------------------ Message: 4 Date: Tue, 9 Sep 2008 08:49:07 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Air purifiers To: "Margaryan, Naira" , Cc: Rex Johnson Message-ID: Content-Type: text/plain; charset="us-ascii" Are you sure that it not the hydrogen peroxide that you may be allergic to. The concentrated (30%) form will bleach your fingers if you are not wearing gloves (or if there is a pinhole in them!) making them quite itchy. I am not aware of any cases of DAB allergy, though you may be the first or, most probably I need to research this more. Is there anyone else who might have a DAB allergy? We probably need to have our OH&S committee look into it. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Tuesday, 9 September 2008 1:39 AM To: histonet@lists.utsouthwestern.edu Cc: mickie25@netzero.net; Rex Johnson Subject: [Histonet] Air purifiers Hi histonetters, Is there any Air purifiers for DAB? I guess I am allergic to that reagent; I can test it after using. I am OK to Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone because I use them in the hood, but DAB is a step in autostainer. Regards, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 5 Date: Tue, 9 Sep 2008 09:43:52 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Air purifiers To: , , "Margaryan, Naira" Cc: Rex Johnson Message-ID: Content-Type: text/plain; charset="iso-8859-1" Ozone may indeed be of some concern. Some research notes: "High levels of atmospheric ozone in Florence air correlated with DNA damage, and to the prevalence of inflammatory pathologies of the upper respiratory tract, although the ozone concentrations were below the Italian recommended attention level. Furthermore, higher levels of DNA damage were correlated with a dysfunction in the ability to maintain a normal epithelial cell structure. These data suggest an association between ozone air levels and damage in the upper respiratory tract. It remains unclear whether ozone itself or other associated pollutants are responsible for the observed alterations." (Environ. Mol. Mutagen. 42:127-135, 2003) "Beagle dogs exposed for 8 hours a day to 3 ppm O3 for 18 months showed cytologic changes that indicate metabolic alterations. The endoplasmic reticulum of the type 2 alveolar epithelial cells in the proximal alveoli were frequently dilated and contained a moderately electron-dense substance having a periodicity of approximately 754 A. This condition was accompanied by a substantial reduction in the lamellar membranes in the characteristic lamellar bodies of the type 2 cells, suggesting a sequestering of protein in the endoplasmic reticulum. In addition, endothelial cells contained paracrystalline arrays of cytoplasmic membranes not observed in control animals" (Am J Pathol. 1973 December; 73(3): 711-726) "The interaction of ozone with some molecules results in an increased production of free radicals. The objective of this study was to identify whether acute ozone exposure to 1-1.5 ppm for 4 h, produced cytological and ultrastructural modifications in the olfactory bulb cells. The results showed that in rats exposed to ozone there was a significant loss of dendritic spines on primary and secondary dendrites of granule cells, whereas the control rats did not present such changes. Besides these exposed cells showed vacuolation of neuronal cytoplasm, swelling of Golgi apparatus and mitochondrion, dilation cisterns of the rough endoplasmic reticulum. These findings suggest that oxidative stress produced by ozone induces alterations in the granule layer of the olfactory bulb, which may be related to functional modifications" (Neuroscience Letters Volume 274, Issue 1, 15 October 1999, Pages 1-4) "Inhalation of the pulmonary irritant ozone is associated with an accumulation of macrophages in the lung. These cells, along with type II epithelial cells, are activated to release increased quantities of hydrogen peroxide and nitric oxide, two reactive mediators that have been implicated in tissue injury" (Am J Respir Cell Mol Biol. 1996 Jun ;14 (6):516-25). "ozone-induced high-protein alveolar edema is pathogenetically linked to pulmonary hyperemia, deficiency of surfactant tubular myelin, and associated lung dysfunctions" (Toxicol Appl Pharmacol Vol. 117 Issue 1 Pg. 37-45 Nov 1992). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, 9 September 2008 2:47 AM To: histonet@lists.utsouthwestern.edu; Margaryan, Naira Cc: Rex Johnson; mickie25@netzero.net Subject: Re: [Histonet] Air purifiers If you can taste DAB after working with it (either weighing of diluting it) it means that you need to wear an AIR?MASK, the air purifier will not be enough. Remember that DAB is cancerigenous. Ren? J. --- On Mon, 9/8/08, Margaryan, Naira wrote: From: Margaryan, Naira Subject: [Histonet] Air purifiers To: histonet@lists.utsouthwestern.edu Cc: "mickie25@netzero.net" <'mickie25@netzero.net'>, "Rex Johnson" Date: Monday, September 8, 2008, 11:39 AM Hi histonetters, Is there any Air purifiers for DAB? I guess I am allergic to that reagent; I can test it after using. I am OK to Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone because I use them in the hood, but DAB is a step in autostainer. Regards, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 6 Date: Tue, 9 Sep 2008 06:03:09 -0500 From: "Leblanc, Barbara Ann" Subject: [Histonet] Varistain Gemini Auto Stainer To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi everyone: We have a Varistain Gemini ES autostainer. We are having variability in the stain quality of the slides. Some slides are stained properly while others are stained lighter. This is sometimes on the same slide (top section stained good; bottom section not good); sometimes some slides in the rack will stain okay and others in the same rack will not. Does anyone have this stainer and has experienced this problem? Thank you, Barbara ------------------------------ Message: 7 Date: Tue, 9 Sep 2008 07:21:15 -0400 From: "Blazek, Linda" Subject: [Histonet] RE: Varistain Gemini Auto Stainer To: "'Leblanc, Barbara Ann'" , "histonet@pathology.swmed.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E39119A05C3@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Barbara, I don't have that stainer but have had that problem from time to time. If you are using recycled alcohol in your last alcohol station or if your alcohol is not really at 100% that may be the problem. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leblanc, Barbara Ann Sent: Tuesday, September 09, 2008 7:03 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Varistain Gemini Auto Stainer Hi everyone: We have a Varistain Gemini ES autostainer. We are having variability in the stain quality of the slides. Some slides are stained properly while others are stained lighter. This is sometimes on the same slide (top section stained good; bottom section not good); sometimes some slides in the rack will stain okay and others in the same rack will not. Does anyone have this stainer and has experienced this problem? Thank you, Barbara _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 9 Sep 2008 05:25:22 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Using Ventana with new antibody lots To: histonet@lists.utsouthwestern.edu, "Sharon.Davis-Devine" Message-ID: <397080.62015.qm@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 This applies to ALL new lots or antibodies, not just for Ventana. 1- when I received a new lot, I tested it WITHOUT? a patient section (just with a + control) before using it with patients. The results were documented in the Abs log. 2- the other question refers to the introduction of a new Ab, something you had not used before. In that case you have to run it with a series of potentially + controls, at different dilution rates to determine the working concentration and the suitability for the purpose it is intended for. It is like a validation test and should be documented also. The pathologist has to sign the validation and concentration tests. Ren? J. --- On Mon, 9/8/08, Sharon.Davis-Devine wrote: From: Sharon.Davis-Devine Subject: [Histonet] Using Ventana with new antibody lots To: histonet@lists.utsouthwestern.edu Date: Monday, September 8, 2008, 5:15 PM Ok, Histonetters I have another question for you. According the CAP checklist question: ANP.22750 Phase II Has the laboratory documented evaluation of new antibody lots and new antibodies, prior to use in patient diagnosis? NOTE: For newly introduced antibodies, staining conditions should be evaluated in cases expected to be positive and negative for the antigen of interest. Ideally, a series of sufficient size should be run to give the laboratory an idea of the sensitivity and specificity of the test. How are you folks handling this question when using Ventana? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 9 Sep 2008 05:43:14 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Varistain Gemini Auto Stainer To: histonet@pathology.swmed.edu, "Leblanc, Barbara Ann" Message-ID: <613411.20328.qm@web65707.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Confronted with an instrument problem that I could not solve by myself, the best option always is to contact the instrument vendor. They usually are able to help. I advise you to do the same. Ren? J. --- On Tue, 9/9/08, Leblanc, Barbara Ann wrote: From: Leblanc, Barbara Ann Subject: [Histonet] Varistain Gemini Auto Stainer To: histonet@pathology.swmed.edu Date: Tuesday, September 9, 2008, 7:03 AM Hi everyone: We have a Varistain Gemini ES autostainer. We are having variability in the stain quality of the slides. Some slides are stained properly while others are stained lighter. This is sometimes on the same slide (top section stained good; bottom section not good); sometimes some slides in the rack will stain okay and others in the same rack will not. Does anyone have this stainer and has experienced this problem? Thank you, Barbara _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 9 Sep 2008 08:46:19 -0500 From: "Gwen Deville" Subject: RE: [Histonet] Using Ventana with new antibody lots To: , , "'Sharon.Davis-Devine'" Message-ID: <003901c91282$708d59d0$51a80d70$@com> Content-Type: text/plain; charset="iso-8859-1" I recently asked CAP for specific details of how antibody validation should be handled since there are many different opinions out there, from lab to lab, tech to tech, and CAP and CLIA. Especially with CLIA enforcing more regs on the anatomic pathology lab. In the past, we in AP did not feel like many of these regs fit in our department, most seemed to be more geared to the clinical laboratory. After experiencing a CLIA visit, I can tell you we have changed our thinking. Dr. Patrick Fitzgibbons responded to my question. There is a book that CAP publishes that every laboratory should have called, "QUALITY MANAGEMENT IN ANATOMIC PATHOLOGY". Chapter 8 deals specifically with IHC and Antibody validation. It details to what extent they should be tested prior to patient testing. Who would know better what is expected in this area? Gwen Deville, Histology Supv. Delta Pathology, Mid-Louisiana Box 30113, 211 Fourth Street Alexandria, LA 71301 ? Work: (318)473-3943 / (318) 473-3180 Pager: (318) 427-5444 Email: gdeville@deltapathology.com ? NOTICE:? This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient.? If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissenmination, distribution, forwarding, printing, or copying of the email or any attached files is strictly prohibited.? If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, September 09, 2008 7:25 AM To: histonet@lists.utsouthwestern.edu; Sharon.Davis-Devine Subject: Re: [Histonet] Using Ventana with new antibody lots This applies to ALL new lots or antibodies, not just for Ventana. 1- when I received a new lot, I tested it WITHOUT? a patient section (just with a + control) before using it with patients. The results were documented in the Abs log. 2- the other question refers to the introduction of a new Ab, something you had not used before. In that case you have to run it with a series of potentially + controls, at different dilution rates to determine the working concentration and the suitability for the purpose it is intended for. It is like a validation test and should be documented also. The pathologist has to sign the validation and concentration tests. Ren? J. --- On Mon, 9/8/08, Sharon.Davis-Devine wrote: From: Sharon.Davis-Devine Subject: [Histonet] Using Ventana with new antibody lots To: histonet@lists.utsouthwestern.edu Date: Monday, September 8, 2008, 5:15 PM Ok, Histonetters I have another question for you. According the CAP checklist question: ANP.22750 Phase II Has the laboratory documented evaluation of new antibody lots and new antibodies, prior to use in patient diagnosis? NOTE: For newly introduced antibodies, staining conditions should be evaluated in cases expected to be positive and negative for the antigen of interest. Ideally, a series of sufficient size should be run to give the laboratory an idea of the sensitivity and specificity of the test. How are you folks handling this question when using Ventana? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Tue, 9 Sep 2008 07:07:20 -0700 From: "Laurie Colbert" Subject: [Histonet] Formaldehyde and scrubs To: Message-ID: <57BE698966D5C54EAE8612E8941D768303ACD1BE@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" Does anyone know of any regulations (OSHA or otherwise) that require employers to provide scrubs to anyone who works with formaldehyde (in order to prevent them from taking contaminated clothing home)? Laurie Colbert ------------------------------ Message: 12 Date: Tue, 9 Sep 2008 07:28:48 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Formaldehyde and scrubs To: histonet@lists.utsouthwestern.edu, Laurie Colbert Message-ID: <422996.27861.qm@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 That is a general OSHA regulation. Exposed clothes to any product or hazardous environment should not be taken home. Ren? J. --- On Tue, 9/9/08, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Formaldehyde and scrubs To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 9, 2008, 10:07 AM Does anyone know of any regulations (OSHA or otherwise) that require employers to provide scrubs to anyone who works with formaldehyde (in order to prevent them from taking contaminated clothing home)? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 9 Sep 2008 09:38:39 -0500 From: Jackie M O'Connor Subject: Re: [Histonet] Formaldehyde and scrubs To: "Laurie Colbert" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We just have fluid resistant labcoats. My daughter is an emergency room tech, and she has to purchase her own scrubs for work, which she wears home, and washes them herself. That's kinda strange to me. Cheap hospital, I guess. "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/09/2008 09:07 AM To cc Subject [Histonet] Formaldehyde and scrubs Does anyone know of any regulations (OSHA or otherwise) that require employers to provide scrubs to anyone who works with formaldehyde (in order to prevent them from taking contaminated clothing home)? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 9 Sep 2008 08:33:36 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Formaldehyde and scrubs To: "Laurie Colbert" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" As far as I can remember, OHSA requires that employees wear a fluid impervious lab coat to protect their clothing and it is the responsibility of the employer to provide and launder the lab coats. Jennifer "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/09/2008 07:10 AM To cc Subject [Histonet] Formaldehyde and scrubs Does anyone know of any regulations (OSHA or otherwise) that require employers to provide scrubs to anyone who works with formaldehyde (in order to prevent them from taking contaminated clothing home)? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Tue, 9 Sep 2008 11:42:02 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] Formaldehyde and scrubs To: "Jennifer MacDonald" , "Laurie Colbert" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB0028AE7E8@e2k3ms1.urmc-sh.rochester.edu> Content-Type: text/plain; charset="iso-8859-1" I believe that the employer can also provide disposable lab aprons that do the same, not just a lab coat. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jennifer MacDonald Sent: Tue 9/9/2008 11:33 AM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Formaldehyde and scrubs As far as I can remember, OHSA requires that employees wear a fluid impervious lab coat to protect their clothing and it is the responsibility of the employer to provide and launder the lab coats. Jennifer "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/09/2008 07:10 AM To cc Subject [Histonet] Formaldehyde and scrubs Does anyone know of any regulations (OSHA or otherwise) that require employers to provide scrubs to anyone who works with formaldehyde (in order to prevent them from taking contaminated clothing home)? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 58, Issue 10 **************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From lblazek <@t> digestivespecialists.com Tue Sep 9 13:53:17 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Sep 9 13:53:29 2008 Subject: [Histonet] RE: Formaldehyde and scrubs In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39119A05CB@IBMB7Exchange.digestivespecialists.com> Lets see..... naked histology tech walking the halls, eating in the cafeteria.... Yummm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph Sent: Tuesday, September 09, 2008 2:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde and scrubs What I like is when people who wear scrubs walk the hallways and even eat in the cafeteria with blood, tissue, and smelling of chemicals on their scrubs. I really like that a lot. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory Mgr One Medimmune Way, Lab 2438-Area 4 Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, September 09, 2008 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 58, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. VEGFR1 and 2 (Hale, Sarah) 2. Re: VEGFR1 and 2 (Nancy Lemke) 3. Using Ventana with new antibody lots (Sharon.Davis-Devine) 4. RE: Air purifiers (Tony Henwood) 5. RE: Air purifiers (Tony Henwood) 6. Varistain Gemini Auto Stainer (Leblanc, Barbara Ann) 7. RE: Varistain Gemini Auto Stainer (Blazek, Linda) 8. Re: Using Ventana with new antibody lots (Rene J Buesa) 9. Re: Varistain Gemini Auto Stainer (Rene J Buesa) 10. RE: Using Ventana with new antibody lots (Gwen Deville) 11. Formaldehyde and scrubs (Laurie Colbert) 12. Re: Formaldehyde and scrubs (Rene J Buesa) 13. Re: Formaldehyde and scrubs (Jackie M O'Connor) 14. Re: Formaldehyde and scrubs (Jennifer MacDonald) 15. RE: Formaldehyde and scrubs (McMahon, Loralee A) ---------------------------------------------------------------------- Message: 1 Date: Mon, 8 Sep 2008 13:36:38 -0400 From: "Hale, Sarah" Subject: [Histonet] VEGFR1 and 2 To: Message-ID: <36830148D2AC3E48A318C1436C89462B088BED78@MED05.med.uvm> Content-Type: text/plain; charset="US-ASCII" Hi! Does anyone have any experience with IHC for VEGFR1 and 2 in rat arteries? Our lab has had trouble getting good staining especially with paraffin sections. Thanks! -Sarah __________________________________________________ Sarah Hale, Ph.D. Postdoctoral Associate Dept. of OB/Gyn and Reproductive Sciences C215 Given Bldg. University of Vermont Burlington, VT 05405 ph: 802.656.1207 email: sarah.hale@uvm.edu ------------------------------ Message: 2 Date: Mon, 08 Sep 2008 13:53:16 -0400 From: "Nancy Lemke" Subject: Re: [Histonet] VEGFR1 and 2 To: "Hale, Sarah" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hi Sarah, I can heartily recommend Santa Cruz sc-6251. FFPE: retrieve in citrate buffer, pH 6.0- boil for 10 minutes, cool for 20. Block, then incubate in sc-6251 at 1:1500 for 30 minutes at room temp. I use Biocare 4+ detection as given on data sheet and Biocare Betazoid DAB for 4 minutes and get beautiful, clean results. I use this in rat sections. Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Hale, Sarah" Sarah.Hale@uvm.edu Date: Mon, 08 Sep 2008 13:37:43 -0400 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VEGFR1 and 2 > Hi! > > Does anyone have any experience with IHC for VEGFR1 and 2 in rat > arteries? Our lab has had trouble getting good staining especially > with paraffin sections. > > Thanks! > -Sarah > > __________________________________________________ > Sarah Hale, Ph.D. > Postdoctoral Associate > Dept. of OB/Gyn and Reproductive Sciences > C215 Given Bldg. > University of Vermont > Burlington, VT 05405 > ph: 802.656.1207 > email: sarah.hale@uvm.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. 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If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== ------------------------------ Message: 3 Date: Mon, 8 Sep 2008 16:15:05 -0500 From: "Sharon.Davis-Devine" Subject: [Histonet] Using Ventana with new antibody lots To: Message-ID: <44780C571F28624DBB446DE55C4D733A021E0A94@EXCHANGEBE1.carle.com> Content-Type: text/plain; charset="us-ascii" Ok, Histonetters I have another question for you. According the CAP checklist question: ANP.22750 Phase II Has the laboratory documented evaluation of new antibody lots and new antibodies, prior to use in patient diagnosis? NOTE: For newly introduced antibodies, staining conditions should be evaluated in cases expected to be positive and negative for the antigen of interest. Ideally, a series of sufficient size should be run to give the laboratory an idea of the sensitivity and specificity of the test. How are you folks handling this question when using Ventana? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com ------------------------------ Message: 4 Date: Tue, 9 Sep 2008 08:49:07 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Air purifiers To: "Margaryan, Naira" , Cc: Rex Johnson Message-ID: Content-Type: text/plain; charset="us-ascii" Are you sure that it not the hydrogen peroxide that you may be allergic to. The concentrated (30%) form will bleach your fingers if you are not wearing gloves (or if there is a pinhole in them!) making them quite itchy. I am not aware of any cases of DAB allergy, though you may be the first or, most probably I need to research this more. Is there anyone else who might have a DAB allergy? We probably need to have our OH&S committee look into it. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Tuesday, 9 September 2008 1:39 AM To: histonet@lists.utsouthwestern.edu Cc: mickie25@netzero.net; Rex Johnson Subject: [Histonet] Air purifiers Hi histonetters, Is there any Air purifiers for DAB? I guess I am allergic to that reagent; I can test it after using. I am OK to Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone because I use them in the hood, but DAB is a step in autostainer. Regards, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 5 Date: Tue, 9 Sep 2008 09:43:52 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Air purifiers To: , , "Margaryan, Naira" Cc: Rex Johnson Message-ID: Content-Type: text/plain; charset="iso-8859-1" Ozone may indeed be of some concern. Some research notes: "High levels of atmospheric ozone in Florence air correlated with DNA damage, and to the prevalence of inflammatory pathologies of the upper respiratory tract, although the ozone concentrations were below the Italian recommended attention level. Furthermore, higher levels of DNA damage were correlated with a dysfunction in the ability to maintain a normal epithelial cell structure. These data suggest an association between ozone air levels and damage in the upper respiratory tract. It remains unclear whether ozone itself or other associated pollutants are responsible for the observed alterations." (Environ. Mol. Mutagen. 42:127-135, 2003) "Beagle dogs exposed for 8 hours a day to 3 ppm O3 for 18 months showed cytologic changes that indicate metabolic alterations. The endoplasmic reticulum of the type 2 alveolar epithelial cells in the proximal alveoli were frequently dilated and contained a moderately electron-dense substance having a periodicity of approximately 754 A. This condition was accompanied by a substantial reduction in the lamellar membranes in the characteristic lamellar bodies of the type 2 cells, suggesting a sequestering of protein in the endoplasmic reticulum. In addition, endothelial cells contained paracrystalline arrays of cytoplasmic membranes not observed in control animals" (Am J Pathol. 1973 December; 73(3): 711-726) "The interaction of ozone with some molecules results in an increased production of free radicals. The objective of this study was to identify whether acute ozone exposure to 1-1.5 ppm for 4 h, produced cytological and ultrastructural modifications in the olfactory bulb cells. The results showed that in rats exposed to ozone there was a significant loss of dendritic spines on primary and secondary dendrites of granule cells, whereas the control rats did not present such changes. Besides these exposed cells showed vacuolation of neuronal cytoplasm, swelling of Golgi apparatus and mitochondrion, dilation cisterns of the rough endoplasmic reticulum. These findings suggest that oxidative stress produced by ozone induces alterations in the granule layer of the olfactory bulb, which may be related to functional modifications" (Neuroscience Letters Volume 274, Issue 1, 15 October 1999, Pages 1-4) "Inhalation of the pulmonary irritant ozone is associated with an accumulation of macrophages in the lung. These cells, along with type II epithelial cells, are activated to release increased quantities of hydrogen peroxide and nitric oxide, two reactive mediators that have been implicated in tissue injury" (Am J Respir Cell Mol Biol. 1996 Jun ;14 (6):516-25). "ozone-induced high-protein alveolar edema is pathogenetically linked to pulmonary hyperemia, deficiency of surfactant tubular myelin, and associated lung dysfunctions" (Toxicol Appl Pharmacol Vol. 117 Issue 1 Pg. 37-45 Nov 1992). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, 9 September 2008 2:47 AM To: histonet@lists.utsouthwestern.edu; Margaryan, Naira Cc: Rex Johnson; mickie25@netzero.net Subject: Re: [Histonet] Air purifiers If you can taste DAB after working with it (either weighing of diluting it) it means that you need to wear an AIR MASK, the air purifier will not be enough. Remember that DAB is cancerigenous. Ren? J. --- On Mon, 9/8/08, Margaryan, Naira wrote: From: Margaryan, Naira Subject: [Histonet] Air purifiers To: histonet@lists.utsouthwestern.edu Cc: "mickie25@netzero.net" <'mickie25@netzero.net'>, "Rex Johnson" Date: Monday, September 8, 2008, 11:39 AM Hi histonetters, Is there any Air purifiers for DAB? I guess I am allergic to that reagent; I can test it after using. I am OK to Formaldehyde, Xylene, Toluene, Glutaraldehyde, Alcohol, Acetone because I use them in the hood, but DAB is a step in autostainer. Regards, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 6 Date: Tue, 9 Sep 2008 06:03:09 -0500 From: "Leblanc, Barbara Ann" Subject: [Histonet] Varistain Gemini Auto Stainer To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi everyone: We have a Varistain Gemini ES autostainer. We are having variability in the stain quality of the slides. Some slides are stained properly while others are stained lighter. This is sometimes on the same slide (top section stained good; bottom section not good); sometimes some slides in the rack will stain okay and others in the same rack will not. Does anyone have this stainer and has experienced this problem? Thank you, Barbara ------------------------------ Message: 7 Date: Tue, 9 Sep 2008 07:21:15 -0400 From: "Blazek, Linda" Subject: [Histonet] RE: Varistain Gemini Auto Stainer To: "'Leblanc, Barbara Ann'" , "histonet@pathology.swmed.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E39119A05C3@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Barbara, I don't have that stainer but have had that problem from time to time. If you are using recycled alcohol in your last alcohol station or if your alcohol is not really at 100% that may be the problem. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leblanc, Barbara Ann Sent: Tuesday, September 09, 2008 7:03 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Varistain Gemini Auto Stainer Hi everyone: We have a Varistain Gemini ES autostainer. We are having variability in the stain quality of the slides. Some slides are stained properly while others are stained lighter. This is sometimes on the same slide (top section stained good; bottom section not good); sometimes some slides in the rack will stain okay and others in the same rack will not. Does anyone have this stainer and has experienced this problem? Thank you, Barbara _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 9 Sep 2008 05:25:22 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Using Ventana with new antibody lots To: histonet@lists.utsouthwestern.edu, "Sharon.Davis-Devine" Message-ID: <397080.62015.qm@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 This applies to ALL new lots or antibodies, not just for Ventana. 1- when I received a new lot, I tested it WITHOUT a patient section (just with a + control) before using it with patients. The results were documented in the Abs log. 2- the other question refers to the introduction of a new Ab, something you had not used before. In that case you have to run it with a series of potentially + controls, at different dilution rates to determine the working concentration and the suitability for the purpose it is intended for. It is like a validation test and should be documented also. The pathologist has to sign the validation and concentration tests. Ren? J. --- On Mon, 9/8/08, Sharon.Davis-Devine wrote: From: Sharon.Davis-Devine Subject: [Histonet] Using Ventana with new antibody lots To: histonet@lists.utsouthwestern.edu Date: Monday, September 8, 2008, 5:15 PM Ok, Histonetters I have another question for you. According the CAP checklist question: ANP.22750 Phase II Has the laboratory documented evaluation of new antibody lots and new antibodies, prior to use in patient diagnosis? NOTE: For newly introduced antibodies, staining conditions should be evaluated in cases expected to be positive and negative for the antigen of interest. Ideally, a series of sufficient size should be run to give the laboratory an idea of the sensitivity and specificity of the test. How are you folks handling this question when using Ventana? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 9 Sep 2008 05:43:14 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Varistain Gemini Auto Stainer To: histonet@pathology.swmed.edu, "Leblanc, Barbara Ann" Message-ID: <613411.20328.qm@web65707.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Confronted with an instrument problem that I could not solve by myself, the best option always is to contact the instrument vendor. They usually are able to help. I advise you to do the same. Ren? J. --- On Tue, 9/9/08, Leblanc, Barbara Ann wrote: From: Leblanc, Barbara Ann Subject: [Histonet] Varistain Gemini Auto Stainer To: histonet@pathology.swmed.edu Date: Tuesday, September 9, 2008, 7:03 AM Hi everyone: We have a Varistain Gemini ES autostainer. We are having variability in the stain quality of the slides. Some slides are stained properly while others are stained lighter. This is sometimes on the same slide (top section stained good; bottom section not good); sometimes some slides in the rack will stain okay and others in the same rack will not. Does anyone have this stainer and has experienced this problem? Thank you, Barbara _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 9 Sep 2008 08:46:19 -0500 From: "Gwen Deville" Subject: RE: [Histonet] Using Ventana with new antibody lots To: , , "'Sharon.Davis-Devine'" Message-ID: <003901c91282$708d59d0$51a80d70$@com> Content-Type: text/plain; charset="iso-8859-1" I recently asked CAP for specific details of how antibody validation should be handled since there are many different opinions out there, from lab to lab, tech to tech, and CAP and CLIA. Especially with CLIA enforcing more regs on the anatomic pathology lab. In the past, we in AP did not feel like many of these regs fit in our department, most seemed to be more geared to the clinical laboratory. After experiencing a CLIA visit, I can tell you we have changed our thinking. Dr. Patrick Fitzgibbons responded to my question. There is a book that CAP publishes that every laboratory should have called, "QUALITY MANAGEMENT IN ANATOMIC PATHOLOGY". Chapter 8 deals specifically with IHC and Antibody validation. It details to what extent they should be tested prior to patient testing. Who would know better what is expected in this area? Gwen Deville, Histology Supv. Delta Pathology, Mid-Louisiana Box 30113, 211 Fourth Street Alexandria, LA 71301 Work: (318)473-3943 / (318) 473-3180 Pager: (318) 427-5444 Email: gdeville@deltapathology.com NOTICE: This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissenmination, distribution, forwarding, printing, or copying of the email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, September 09, 2008 7:25 AM To: histonet@lists.utsouthwestern.edu; Sharon.Davis-Devine Subject: Re: [Histonet] Using Ventana with new antibody lots This applies to ALL new lots or antibodies, not just for Ventana. 1- when I received a new lot, I tested it WITHOUT a patient section (just with a + control) before using it with patients. The results were documented in the Abs log. 2- the other question refers to the introduction of a new Ab, something you had not used before. In that case you have to run it with a series of potentially + controls, at different dilution rates to determine the working concentration and the suitability for the purpose it is intended for. It is like a validation test and should be documented also. The pathologist has to sign the validation and concentration tests. Ren? J. --- On Mon, 9/8/08, Sharon.Davis-Devine wrote: From: Sharon.Davis-Devine Subject: [Histonet] Using Ventana with new antibody lots To: histonet@lists.utsouthwestern.edu Date: Monday, September 8, 2008, 5:15 PM Ok, Histonetters I have another question for you. According the CAP checklist question: ANP.22750 Phase II Has the laboratory documented evaluation of new antibody lots and new antibodies, prior to use in patient diagnosis? NOTE: For newly introduced antibodies, staining conditions should be evaluated in cases expected to be positive and negative for the antigen of interest. Ideally, a series of sufficient size should be run to give the laboratory an idea of the sensitivity and specificity of the test. How are you folks handling this question when using Ventana? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Tue, 9 Sep 2008 07:07:20 -0700 From: "Laurie Colbert" Subject: [Histonet] Formaldehyde and scrubs To: Message-ID: <57BE698966D5C54EAE8612E8941D768303ACD1BE@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" Does anyone know of any regulations (OSHA or otherwise) that require employers to provide scrubs to anyone who works with formaldehyde (in order to prevent them from taking contaminated clothing home)? Laurie Colbert ------------------------------ Message: 12 Date: Tue, 9 Sep 2008 07:28:48 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Formaldehyde and scrubs To: histonet@lists.utsouthwestern.edu, Laurie Colbert Message-ID: <422996.27861.qm@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 That is a general OSHA regulation. Exposed clothes to any product or hazardous environment should not be taken home. Ren? J. --- On Tue, 9/9/08, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Formaldehyde and scrubs To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 9, 2008, 10:07 AM Does anyone know of any regulations (OSHA or otherwise) that require employers to provide scrubs to anyone who works with formaldehyde (in order to prevent them from taking contaminated clothing home)? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 9 Sep 2008 09:38:39 -0500 From: Jackie M O'Connor Subject: Re: [Histonet] Formaldehyde and scrubs To: "Laurie Colbert" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We just have fluid resistant labcoats. My daughter is an emergency room tech, and she has to purchase her own scrubs for work, which she wears home, and washes them herself. That's kinda strange to me. Cheap hospital, I guess. "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/09/2008 09:07 AM To cc Subject [Histonet] Formaldehyde and scrubs Does anyone know of any regulations (OSHA or otherwise) that require employers to provide scrubs to anyone who works with formaldehyde (in order to prevent them from taking contaminated clothing home)? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 9 Sep 2008 08:33:36 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Formaldehyde and scrubs To: "Laurie Colbert" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" As far as I can remember, OHSA requires that employees wear a fluid impervious lab coat to protect their clothing and it is the responsibility of the employer to provide and launder the lab coats. Jennifer "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/09/2008 07:10 AM To cc Subject [Histonet] Formaldehyde and scrubs Does anyone know of any regulations (OSHA or otherwise) that require employers to provide scrubs to anyone who works with formaldehyde (in order to prevent them from taking contaminated clothing home)? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Tue, 9 Sep 2008 11:42:02 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] Formaldehyde and scrubs To: "Jennifer MacDonald" , "Laurie Colbert" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB0028AE7E8@e2k3ms1.urmc-sh.rochester.edu> Content-Type: text/plain; charset="iso-8859-1" I believe that the employer can also provide disposable lab aprons that do the same, not just a lab coat. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jennifer MacDonald Sent: Tue 9/9/2008 11:33 AM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Formaldehyde and scrubs As far as I can remember, OHSA requires that employees wear a fluid impervious lab coat to protect their clothing and it is the responsibility of the employer to provide and launder the lab coats. Jennifer "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 09/09/2008 07:10 AM To cc Subject [Histonet] Formaldehyde and scrubs Does anyone know of any regulations (OSHA or otherwise) that require employers to provide scrubs to anyone who works with formaldehyde (in order to prevent them from taking contaminated clothing home)? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 58, Issue 10 **************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Tue Sep 9 15:22:19 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Sep 9 15:22:24 2008 Subject: [Histonet] Re: Formaldehyde and scrubs Message-ID: Truly amazing. Formaldehyde is what puts the press in permanent press fabrics. A number of years ago I visited a small clothing factory in a southeastern state that doesn't enforce environmental regulations. Bolts of cloth hung from the ceiling, ready for use. The smell of formaldehyde was so strong I could have closed my eyes and imagined I was grossing. Bob Richmond Samurai Pathologist Knoxville TN From Margaret.Perry <@t> sdstate.edu Tue Sep 9 15:22:27 2008 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Tue Sep 9 15:22:33 2008 Subject: [Histonet] methylene blue Message-ID: Help! We have been asked to do a methylene blue stain for Myxosporidia and don't have a procedure. Of course the pathologist wants it quickly. I can't get into the archives. It must be down right now. Can someone help us? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From leiker <@t> buffalo.edu Tue Sep 9 15:54:43 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Tue Sep 9 15:54:49 2008 Subject: [Histonet] Manual Paraffin Embedding In-Reply-To: References: Message-ID: <6089D76726392CD3B3399BFC@bchwxp2702.ad.med.buffalo.edu> Does anyone process and embed tissues manually instead of using automated and expensive equipment? Can you tell me how you do it? Thanks. Merced From contact <@t> excaliburpathology.com Tue Sep 9 16:19:57 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Sep 9 16:20:01 2008 Subject: [Histonet] Manual Paraffin Embedding Message-ID: <548890.39203.qm@web1116.biz.mail.sk1.yahoo.com> I sometimes still use a paraffin dispenser for really large blocks. It looks like a big coffee maker, like caterers use. You will?still need molds. Embedding centers work on the same principle, they just incorporate the melted paraffin bay and dispenser with a cold plate on the side. Paula Pierce ----- Original Message ---- From: Merced Leiker To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 9, 2008 3:54:43 PM Subject: [Histonet] Manual Paraffin Embedding Does anyone process and embed tissues manually instead of using automated and expensive equipment?? Can you tell me how you do it?? Thanks. Merced _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Tue Sep 9 17:21:02 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Sep 9 17:21:57 2008 Subject: [Histonet] Manual Paraffin Embedding References: <6089D76726392CD3B3399BFC@bchwxp2702.ad.med.buffalo.edu> Message-ID: <9A9BD3D0B0284D23904E676EA082BD4F@Compaq> I don't do this anymore, nor for 40 years now, but this is what we used to do aeons ago. 1. Fix in 10% NBF for 48 hours. 2 Rinse off excess with tap water for 1 minute. 3. Select pieces of tissue with maximum dimensions of 2cm x 1.5 cm x 0.3 cm. 4. Place into cassettes if you have them, then into a large container. If you do not have cassettes, place into small jars, each case in a different jar. Place a label in each cassette or each jar with the case ID. 5. Cover the tissue or cassettes with 70% ethanol, methylated spirits or isopropanol, agitate gently. Leave overnight. 6. Next morning, replace the 70& alcohol with 85% alcohol, leave for the day, agitating gently periodically. 7. Before leaving in the evening, replace the alcohol with 95% alcohol, agitate gently and leave overnight. 8. Next morning, replace the alcohol with 100% alcohol, gently agitate periodically. Repeat at noon and before you 9. leave for the evening, gently agitating. 10. Next morning, replace the alcohol with clearant, preferably xylene or toluene. Leave for one hour, gently agitating periodically. 11. Repeat the clearant twice more, agitate gently. 12. Place into premelted paraffin wax for 1 hour at 65C. Check periodically and when all congealed wax has remelted, agitate gently. 13. Repeat at least twice more, preferably under vacuum - not too strong. Some technologists used to leave the final change overnight. Doing so doesn't do much harm and improves penetration. Agitation can't be done under vacuum, so release the vacuum periodically, agitate and reapply it. 14. Block out into molds. (If you do not have molds, use a metal - tinned steel or aluminum - lid with a depth of 1cm. LIGHTLY coat it with glycerol first.) Place a thin (3mm) layer of hot wax in the mold and place the tissue, with the surface to be sectioned down, into it. Top up the mold with wax so there is at least 2-3 mm wax over the top of the tissue. Put the ID label conspiciously next to the tissue. Do NOT block out more than one tissue or case without placing the ID labels, this WILL lead to serious identification errors. Do all this by the oven and keep the door closed as much as possible. Work fast so that the wax around the tissues does not begin to congeal as that causes problems during sectioning and floating out. You must get the wax around the tissue and the wax in the mold to blend completely, so if the wax congeals around the tissue, leave it to remelt before blocking out. Many of us used to keep a bunsen burner alight and flame the top of the molds to keep the wax molten - a practice probably considered unsafe now. 15. Allow the wax in the mold to skin over thoroughly, then GENTLY lower into cold tap water to cool. 16. When completely cold and solid, use a heavy knife to score and trim the wax blocks. 17. To section, melt the trimmed block onto the block holder. Bryan Llewellyn ----- Original Message ----- From: "Merced Leiker" To: Sent: Tuesday, September 09, 2008 1:54 PM Subject: [Histonet] Manual Paraffin Embedding > Does anyone process and embed tissues manually instead of using automated > and expensive equipment? Can you tell me how you do it? Thanks. > > Merced > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccrowder <@t> vetmed.lsu.edu Tue Sep 9 17:54:41 2008 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Tue Sep 9 17:57:04 2008 Subject: [Histonet] Re: Histonet Digest, Vol 58, Issue 8 In-Reply-To: References: Message-ID: Maxim - You sent me an e-mail in August. I replied several times, but the e-mail did not go through. Is your address complete or is there another I can use? Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 -----Original Message----- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Date: Sun, 07 Sep 2008 12:02:56 -0500 Subject: Histonet Digest, Vol 58, Issue 8 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet [http://lists.utsouthwestern.edu/mailman/listinfo/histonet] or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: reagent question (Maxim_71@mail.ru) ---------------------------------------------------------------------- Message: 1 Date: Sat, 6 Sep 2008 23:38:42 +0400 From: Maxim_71@mail.ru Subject: RE: [Histonet] reagent question To: pathology.histology@gmail.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <03082354.20080906233842@mail.ru> Content-Type: text/plain; charset=us-ascii More than one year we uses isopropanol as dehydratant and enjoy for quality of specimens, which processed with this reagent. It is very useful for bone, cartillage, uterus, breast, colon, skin, eyeball and other difficult specimens. Some unpleasant odor of "cat urine" does not disturb to use it in manual processing. We believes, that will be time, when we will have a vacuum processor... For slides we use acetone as H&E as SS. We stain all our slides manually. Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [http://lists.utsouthwestern.edu/mailman/listinfo/histonet] End of Histonet Digest, Vol 58, Issue 8 *************************************** From rjbuesa <@t> yahoo.com Wed Sep 10 07:00:17 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 10 07:00:27 2008 Subject: [Histonet] methylene blue In-Reply-To: Message-ID: <285564.59557.qm@web65708.mail.ac4.yahoo.com> Methylene blue does not require anything special, this stain never fails. Just hydrate your section, stain with 1% aq. methBlue for 5 minutes, wash and dehydrate QUICKLYLY cover and that is all. wrote: From: Perry, Margaret Subject: [Histonet] methylene blue To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 9, 2008, 4:22 PM Help! We have been asked to do a methylene blue stain for Myxosporidia and don't have a procedure. Of course the pathologist wants it quickly. I can't get into the archives. It must be down right now. Can someone help us? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Sep 10 07:09:14 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 10 07:09:33 2008 Subject: [Histonet] Manual Paraffin Embedding In-Reply-To: <6089D76726392CD3B3399BFC@bchwxp2702.ad.med.buffalo.edu> Message-ID: <577961.55420.qm@web65715.mail.ac4.yahoo.com> Merced: Processing and embedding tissues manually is still done occasionally here in the US and specially abroad. As a matter of fact 2% us US labs and 14% of foreign labs routinely manually process their tissues. ? It implies running the tissues through all the dehydration, clearing and infiltration steps to finally prepare the blocks, also manually, using melted paraffin and dispensed in paper molds, or using Leuckhart rectangles. The description would be very long for an e-mail, so my advise is to get a histotechnique book, like Bolles-Lee's "Microtomist Vade-Mecum", or Peter Gray's "The Microtomist's formulary and guide", both are for sale at Amazon.com/books or you may find a copy at the University of Buffalo. Ren? J. --- On Tue, 9/9/08, Merced Leiker wrote: From: Merced Leiker Subject: [Histonet] Manual Paraffin Embedding To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 9, 2008, 4:54 PM Does anyone process and embed tissues manually instead of using automated and expensive equipment? Can you tell me how you do it? Thanks. Merced _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Wed Sep 10 08:31:17 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Sep 10 08:31:22 2008 Subject: [Histonet] Manual Paraffin Embedding In-Reply-To: <548890.39203.qm@web1116.biz.mail.sk1.yahoo.com> References: <548890.39203.qm@web1116.biz.mail.sk1.yahoo.com> Message-ID: We just use melted paraffin in a vacuum oven. The paraffin is kept at 60C in small glass petri dishes and is moved from dish to dish every half hour for about 3 hours. Then you fill a plastic mold halfway, let the paraffin cool until it's hard enough to pour more on without it melting again, but not fully cooled, put in your tissue, cover it with melted paraffin and let it cool overnight. You don't need the vacuum oven, it just gets rid of bubbles in the paraffin. You also need to use heated instruments (we just warm them briefly in an alcohol lamp flame) when transferring the embryo in paraffin. Emily -- An overcivilized people grow complacent and careless and leave the door open for a tribe of fanatical savages, through a mixture of luck, treachery, and the foulest inhumanity, to usurp their place for a few years. -Richard Adams, "Shardik", 1974 From mpb1211 <@t> uwyo.edu Tue Sep 9 15:50:33 2008 From: mpb1211 <@t> uwyo.edu (Mary P. Brownson) Date: Wed Sep 10 08:40:05 2008 Subject: [Histonet] Paraformaldehyde Message-ID: <85F291B637727346BA8274B0A43C24FF02946E2C@TELEGRAPH1.uwyo.edu> Hello, I plan to use paraformaldehyde for perfusion of rat brains. My question is; what grade paraformaldehde should I be using? Reagent grade, 95% or other? Or does it not matter? Thank you, Mary Brownson School of Pharmacy University of Wyoming Laramie, WY 82070 mpb1211@uwyo.edu From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Sep 10 09:10:35 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Sep 10 09:10:44 2008 Subject: [Histonet] Manual Paraffin Embedding Message-ID: <86ADE4EB583CE64799A9924684A0FBBF054834F2@wahtntex2.waht.swest.nhs.uk> Does anyone process and embed tissues manually instead of using automated and expensive equipment? Can you tell me how you do it? Thanks. Merced When I was a lad I used to manual process Brains and other stuff. In retrospect I wonder why we use the same processing schedules for disparate organs. We ought to have a kidney schedule and a heart schedule specifically for each organ type. I always knew a tissue was processed adequately as it changed in its translucency. If the was water still present in the tissue you could see its presence in the clearing agent and smell clearing agent in the wax. However you exposed yourself to carcinogenic vapours and I guess that it is right and proper we automated and contained. I wonder if that's why I've grown two heads? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From cmiller <@t> physlab.com Wed Sep 10 10:06:56 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Sep 10 10:07:06 2008 Subject: [Histonet] Histology Positions Message-ID: <000001c91356$de4ccc60$3d02a8c0@plab.local> Is there anyone looking for a Histotech or a Histology Supervisor in the Omaha, Council Bluffs or Lincoln area?? Let me know. Thanks, Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From RFISHER <@t> gbmc.org Wed Sep 10 11:16:54 2008 From: RFISHER <@t> gbmc.org (RENEE FISHER) Date: Wed Sep 10 11:17:10 2008 Subject: [Histonet] Help Message-ID: <48C7BAB5.5C87.00CA.0@gbmc.org> please un scribe _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. From RFISHER <@t> gbmc.org Wed Sep 10 11:19:12 2008 From: RFISHER <@t> gbmc.org (RENEE FISHER) Date: Wed Sep 10 11:19:28 2008 Subject: [Histonet] please un subscribe Message-ID: <48C7BB3F.5C87.00CA.0@gbmc.org> please un subscribe _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. From Bauer.Karen <@t> mayo.edu Wed Sep 10 11:58:09 2008 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Wed Sep 10 11:58:25 2008 Subject: [Histonet] Recyclers Message-ID: Hello, I'm in the market to demo some recyclers. Any vendors are welcome to contact me personally. I've already contacted CBG and BR Instruments, so vendors from those areas need not reply to this message. Anyone on Histonet that would like to give me their likes and dislikes of certain recyclers, I'm all ears. Thanks much, Karen Karen L. Bauer HT(ASCP) Department of Pathology Histology Section Chief Luther Hospital Eau Claire, WI 715-838-3205 ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From rjbuesa <@t> yahoo.com Wed Sep 10 12:10:42 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 10 12:10:46 2008 Subject: [Histonet] Paraformaldehyde In-Reply-To: <85F291B637727346BA8274B0A43C24FF02946E2C@TELEGRAPH1.uwyo.edu> Message-ID: <978708.80996.qm@web65704.mail.ac4.yahoo.com> It does not really matter, BUT it will have to be taken into consideration when calculating the final concentraiton you want to use. Ren? J. --- On Tue, 9/9/08, Mary P. Brownson wrote: From: Mary P. Brownson Subject: [Histonet] Paraformaldehyde To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 9, 2008, 4:50 PM Hello, I plan to use paraformaldehyde for perfusion of rat brains. My question is; what grade paraformaldehde should I be using? Reagent grade, 95% or other? Or does it not matter? Thank you, Mary Brownson School of Pharmacy University of Wyoming Laramie, WY 82070 mpb1211@uwyo.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.D.Renko <@t> osfhealthcare.org Wed Sep 10 13:45:47 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Wed Sep 10 13:46:25 2008 Subject: [Histonet] re: Bedside procedure for FNA in US and CT Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09E147@pmc-rfd-mx01.intranet.osfnet.org> Looking for someone out there who is performing assistance bedside for radiology and ultrasound with a pathologist and diff quik stains. Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From paintedsplashes <@t> yahoo.com Wed Sep 10 13:56:56 2008 From: paintedsplashes <@t> yahoo.com (Jeanne Clark) Date: Wed Sep 10 13:57:03 2008 Subject: [Histonet] Re: Histonet Digest, Vol 58, Issue 12 Recyclers Message-ID: <157912.99063.qm@web30701.mail.mud.yahoo.com> I used a CBG recycler for years.....many years and absolutely loved it!? Worked great, excellent recovery (xylene) and minimal maintenance. Great (people) support from the company too. ? Jeanne Jeanne Clark HT/MLT (ASCP) Pathology Manager Mission Hospitals Asheville, NC 423-612-1213 ? ? --- On Wed, 9/10/08, histonet-request@lists.utsouthwestern.edu wrote: From: histonet-request@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 58, Issue 12 To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 10, 2008, 1:21 PM Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Manual Paraffin Embedding (Merced Leiker) 2. Manual Paraffin Embedding (Paula Pierce) 3. Re: Manual Paraffin Embedding (Bryan Llewellyn) 4. Re: Histonet Digest, Vol 58, Issue 8 (Cheryl Crowder) 5. Re: methylene blue (Rene J Buesa) 6. Re: Manual Paraffin Embedding (Rene J Buesa) 7. Re: Manual Paraffin Embedding (Emily Sours) 8. Paraformaldehyde (Mary P. Brownson) 9. RE: Manual Paraffin Embedding (Kemlo Rogerson) 10. Histology Positions (Cheri Miller) 11. Help (RENEE FISHER) 12. please un subscribe (RENEE FISHER) 13. Recyclers (Bauer, Karen) ---------------------------------------------------------------------- Message: 1 Date: Tue, 09 Sep 2008 16:54:43 -0400 From: Merced Leiker Subject: [Histonet] Manual Paraffin Embedding To: histonet@lists.utsouthwestern.edu Message-ID: <6089D76726392CD3B3399BFC@bchwxp2702.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed Does anyone process and embed tissues manually instead of using automated and expensive equipment? Can you tell me how you do it? Thanks. Merced ------------------------------ Message: 2 Date: Tue, 9 Sep 2008 14:19:57 -0700 (PDT) From: Paula Pierce Subject: [Histonet] Manual Paraffin Embedding To: Merced Leiker , Histonet Message-ID: <548890.39203.qm@web1116.biz.mail.sk1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I sometimes still use a paraffin dispenser for really large blocks. It looks like a big coffee maker, like caterers use. You will?still need molds. Embedding centers work on the same principle, they just incorporate the melted paraffin bay and dispenser with a cold plate on the side. Paula Pierce ----- Original Message ---- From: Merced Leiker To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 9, 2008 3:54:43 PM Subject: [Histonet] Manual Paraffin Embedding Does anyone process and embed tissues manually instead of using automated and expensive equipment?? Can you tell me how you do it?? Thanks. Merced _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 9 Sep 2008 15:21:02 -0700 From: "Bryan Llewellyn" Subject: Re: [Histonet] Manual Paraffin Embedding To: Message-ID: <9A9BD3D0B0284D23904E676EA082BD4F@Compaq> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=response I don't do this anymore, nor for 40 years now, but this is what we used to do aeons ago. 1. Fix in 10% NBF for 48 hours. 2 Rinse off excess with tap water for 1 minute. 3. Select pieces of tissue with maximum dimensions of 2cm x 1.5 cm x 0.3 cm. 4. Place into cassettes if you have them, then into a large container. If you do not have cassettes, place into small jars, each case in a different jar. Place a label in each cassette or each jar with the case ID. 5. Cover the tissue or cassettes with 70% ethanol, methylated spirits or isopropanol, agitate gently. Leave overnight. 6. Next morning, replace the 70& alcohol with 85% alcohol, leave for the day, agitating gently periodically. 7. Before leaving in the evening, replace the alcohol with 95% alcohol, agitate gently and leave overnight. 8. Next morning, replace the alcohol with 100% alcohol, gently agitate periodically. Repeat at noon and before you 9. leave for the evening, gently agitating. 10. Next morning, replace the alcohol with clearant, preferably xylene or toluene. Leave for one hour, gently agitating periodically. 11. Repeat the clearant twice more, agitate gently. 12. Place into premelted paraffin wax for 1 hour at 65C. Check periodically and when all congealed wax has remelted, agitate gently. 13. Repeat at least twice more, preferably under vacuum - not too strong. Some technologists used to leave the final change overnight. Doing so doesn't do much harm and improves penetration. Agitation can't be done under vacuum, so release the vacuum periodically, agitate and reapply it. 14. Block out into molds. (If you do not have molds, use a metal - tinned steel or aluminum - lid with a depth of 1cm. LIGHTLY coat it with glycerol first.) Place a thin (3mm) layer of hot wax in the mold and place the tissue, with the surface to be sectioned down, into it. Top up the mold with wax so there is at least 2-3 mm wax over the top of the tissue. Put the ID label conspiciously next to the tissue. Do NOT block out more than one tissue or case without placing the ID labels, this WILL lead to serious identification errors. Do all this by the oven and keep the door closed as much as possible. Work fast so that the wax around the tissues does not begin to congeal as that causes problems during sectioning and floating out. You must get the wax around the tissue and the wax in the mold to blend completely, so if the wax congeals around the tissue, leave it to remelt before blocking out. Many of us used to keep a bunsen burner alight and flame the top of the molds to keep the wax molten - a practice probably considered unsafe now. 15. Allow the wax in the mold to skin over thoroughly, then GENTLY lower into cold tap water to cool. 16. When completely cold and solid, use a heavy knife to score and trim the wax blocks. 17. To section, melt the trimmed block onto the block holder. Bryan Llewellyn ----- Original Message ----- From: "Merced Leiker" To: Sent: Tuesday, September 09, 2008 1:54 PM Subject: [Histonet] Manual Paraffin Embedding > Does anyone process and embed tissues manually instead of using automated > and expensive equipment? Can you tell me how you do it? Thanks. > > Merced > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 09 Sep 2008 17:54:41 -0500 From: "Cheryl Crowder" Subject: [Histonet] Re: Histonet Digest, Vol 58, Issue 8 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" Maxim - You sent me an e-mail in August. I replied several times, but the e-mail did not go through. Is your address complete or is there another I can use? Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 -----Original Message----- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Date: Sun, 07 Sep 2008 12:02:56 -0500 Subject: Histonet Digest, Vol 58, Issue 8 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet [http://lists.utsouthwestern.edu/mailman/listinfo/histonet] or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: reagent question (Maxim_71@mail.ru) ---------------------------------------------------------------------- Message: 1 Date: Sat, 6 Sep 2008 23:38:42 +0400 From: Maxim_71@mail.ru Subject: RE: [Histonet] reagent question To: pathology.histology@gmail.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <03082354.20080906233842@mail.ru> Content-Type: text/plain; charset=us-ascii More than one year we uses isopropanol as dehydratant and enjoy for quality of specimens, which processed with this reagent. It is very useful for bone, cartillage, uterus, breast, colon, skin, eyeball and other difficult specimens. Some unpleasant odor of "cat urine" does not disturb to use it in manual processing. We believes, that will be time, when we will have a vacuum processor... For slides we use acetone as H&E as SS. We stain all our slides manually. Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [http://lists.utsouthwestern.edu/mailman/listinfo/histonet] End of Histonet Digest, Vol 58, Issue 8 *************************************** ------------------------------ Message: 5 Date: Wed, 10 Sep 2008 05:00:17 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] methylene blue To: histonet@lists.utsouthwestern.edu, "Perry, Margaret" Message-ID: <285564.59557.qm@web65708.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Methylene blue does not require anything special, this stain never fails. Just hydrate your section, stain with 1% aq. methBlue for 5 minutes, wash and dehydrate QUICKLYLY cover and that is all. wrote: From: Perry, Margaret Subject: [Histonet] methylene blue To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 9, 2008, 4:22 PM Help! We have been asked to do a methylene blue stain for Myxosporidia and don't have a procedure. Of course the pathologist wants it quickly. I can't get into the archives. It must be down right now. Can someone help us? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 10 Sep 2008 05:09:14 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Manual Paraffin Embedding To: histonet@lists.utsouthwestern.edu, Merced Leiker Message-ID: <577961.55420.qm@web65715.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Merced: Processing and embedding tissues manually is still done occasionally here in the US and specially abroad. As a matter of fact 2% us US labs and 14% of foreign labs routinely manually process their tissues. ? It implies running the tissues through all the dehydration, clearing and infiltration steps to finally prepare the blocks, also manually, using melted paraffin and dispensed in paper molds, or using Leuckhart rectangles. The description would be very long for an e-mail, so my advise is to get a histotechnique book, like Bolles-Lee's "Microtomist Vade-Mecum", or Peter Gray's "The Microtomist's formulary and guide", both are for sale at Amazon.com/books or you may find a copy at the University of Buffalo. Ren? J. --- On Tue, 9/9/08, Merced Leiker wrote: From: Merced Leiker Subject: [Histonet] Manual Paraffin Embedding To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 9, 2008, 4:54 PM Does anyone process and embed tissues manually instead of using automated and expensive equipment? Can you tell me how you do it? Thanks. Merced _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 10 Sep 2008 09:31:17 -0400 From: "Emily Sours" Subject: Re: [Histonet] Manual Paraffin Embedding To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 We just use melted paraffin in a vacuum oven. The paraffin is kept at 60C in small glass petri dishes and is moved from dish to dish every half hour for about 3 hours. Then you fill a plastic mold halfway, let the paraffin cool until it's hard enough to pour more on without it melting again, but not fully cooled, put in your tissue, cover it with melted paraffin and let it cool overnight. You don't need the vacuum oven, it just gets rid of bubbles in the paraffin. You also need to use heated instruments (we just warm them briefly in an alcohol lamp flame) when transferring the embryo in paraffin. Emily -- An overcivilized people grow complacent and careless and leave the door open for a tribe of fanatical savages, through a mixture of luck, treachery, and the foulest inhumanity, to usurp their place for a few years. -Richard Adams, "Shardik", 1974 ------------------------------ Message: 8 Date: Tue, 9 Sep 2008 14:50:33 -0600 From: "Mary P. Brownson" Subject: [Histonet] Paraformaldehyde To: Message-ID: <85F291B637727346BA8274B0A43C24FF02946E2C@TELEGRAPH1.uwyo.edu> Content-Type: text/plain; charset="us-ascii" Hello, I plan to use paraformaldehyde for perfusion of rat brains. My question is; what grade paraformaldehde should I be using? Reagent grade, 95% or other? Or does it not matter? Thank you, Mary Brownson School of Pharmacy University of Wyoming Laramie, WY 82070 mpb1211@uwyo.edu ------------------------------ Message: 9 Date: Wed, 10 Sep 2008 15:10:35 +0100 From: "Kemlo Rogerson" Subject: RE: [Histonet] Manual Paraffin Embedding To: "Merced Leiker" , Message-ID: <86ADE4EB583CE64799A9924684A0FBBF054834F2@wahtntex2.waht.swest.nhs.uk> Content-Type: text/plain; charset="us-ascii" Does anyone process and embed tissues manually instead of using automated and expensive equipment? Can you tell me how you do it? Thanks. Merced When I was a lad I used to manual process Brains and other stuff. In retrospect I wonder why we use the same processing schedules for disparate organs. We ought to have a kidney schedule and a heart schedule specifically for each organ type. I always knew a tissue was processed adequately as it changed in its translucency. If the was water still present in the tissue you could see its presence in the clearing agent and smell clearing agent in the wax. However you exposed yourself to carcinogenic vapours and I guess that it is right and proper we automated and contained. I wonder if that's why I've grown two heads? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation ------------------------------ Message: 10 Date: Wed, 10 Sep 2008 10:06:56 -0500 From: "Cheri Miller" Subject: [Histonet] Histology Positions To: Cc: Message-ID: <000001c91356$de4ccc60$3d02a8c0@plab.local> Content-Type: text/plain; charset="us-ascii" Is there anyone looking for a Histotech or a Histology Supervisor in the Omaha, Council Bluffs or Lincoln area?? Let me know. Thanks, Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 11 Date: Wed, 10 Sep 2008 12:16:54 -0400 From: "RENEE FISHER" Subject: [Histonet] Help To: Message-ID: <48C7BAB5.5C87.00CA.0@gbmc.org> Content-Type: text/plain; charset=US-ASCII please un scribe _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. ------------------------------ Message: 12 Date: Wed, 10 Sep 2008 12:19:12 -0400 From: "RENEE FISHER" Subject: [Histonet] please un subscribe To: Message-ID: <48C7BB3F.5C87.00CA.0@gbmc.org> Content-Type: text/plain; charset=US-ASCII please un subscribe _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. ------------------------------ Message: 13 Date: Wed, 10 Sep 2008 11:58:09 -0500 From: "Bauer, Karen" Subject: [Histonet] Recyclers To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello, I'm in the market to demo some recyclers. Any vendors are welcome to contact me personally. I've already contacted CBG and BR Instruments, so vendors from those areas need not reply to this message. Anyone on Histonet that would like to give me their likes and dislikes of certain recyclers, I'm all ears. Thanks much, Karen Karen L. Bauer HT(ASCP) Department of Pathology Histology Section Chief Luther Hospital Eau Claire, WI 715-838-3205 ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 58, Issue 12 **************************************** From leiker <@t> buffalo.edu Wed Sep 10 14:05:31 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Wed Sep 10 14:05:37 2008 Subject: [Histonet] Manual Paraffin Embedding In-Reply-To: <9A9BD3D0B0284D23904E676EA082BD4F@Compaq> References: <6089D76726392CD3B3399BFC@bchwxp2702.ad.med.buffalo.edu> <9A9BD3D0B0284D23904E676EA082BD4F@Compaq> Message-ID: <8EEF817B88BE45B668DB4F25@bchwxp2702.ad.med.buffalo.edu> Thanks for all the detail, Bryan! We will try this and see how it works. Thanks to everyone else who replied as well, your tips are taken into consideration, too. Merced --On Tuesday, September 09, 2008 3:21 PM -0700 Bryan Llewellyn wrote: > I don't do this anymore, nor for 40 years now, but this is what we used > to do aeons ago. > > 1. Fix in 10% NBF for 48 hours. > 2 Rinse off excess with tap water for 1 minute. > 3. Select pieces of tissue with maximum dimensions of 2cm x 1.5 cm x 0.3 > cm. > 4. Place into cassettes if you have them, then into a large container. > If you do not have cassettes, place into small jars, each case in a > different jar. Place a label in each cassette or each jar with the case > ID. > 5. Cover the tissue or cassettes with 70% ethanol, methylated spirits or > isopropanol, agitate gently. Leave overnight. > 6. Next morning, replace the 70& alcohol with 85% alcohol, leave for the > day, agitating gently periodically. > 7. Before leaving in the evening, replace the alcohol with 95% alcohol, > agitate gently and leave overnight. > 8. Next morning, replace the alcohol with 100% alcohol, gently agitate > periodically. Repeat at noon and before you 9. leave for the evening, > gently agitating. > 10. Next morning, replace the alcohol with clearant, preferably xylene or > toluene. Leave for one hour, gently agitating periodically. > 11. Repeat the clearant twice more, agitate gently. > 12. Place into premelted paraffin wax for 1 hour at 65C. Check > periodically and when all congealed wax has remelted, agitate gently. > 13. Repeat at least twice more, preferably under vacuum - not too strong. > Some technologists used to leave the final change overnight. Doing so > doesn't do much harm and improves penetration. Agitation can't be done > under vacuum, so release the vacuum periodically, agitate and reapply it. > 14. Block out into molds. (If you do not have molds, use a metal - > tinned steel or aluminum - lid with a depth of 1cm. LIGHTLY coat it with > glycerol first.) Place a thin (3mm) layer of hot wax in the mold and > place the tissue, with the surface to be sectioned down, into it. Top up > the mold with wax so there is at least 2-3 mm wax over the top of the > tissue. Put the ID label conspiciously next to the tissue. Do NOT block > out more than one tissue or case without placing the ID labels, this WILL > lead to serious identification errors. Do all this by the oven and keep > the door closed as much as possible. Work fast so that the wax around > the tissues does not begin to congeal as that causes problems during > sectioning and floating out. You must get the wax around the tissue and > the wax in the mold to blend completely, so if the wax congeals around > the tissue, leave it to remelt before blocking out. Many of us used to > keep a bunsen burner alight and flame the top of the molds to keep the > wax molten - a practice probably considered unsafe now. > 15. Allow the wax in the mold to skin over thoroughly, then GENTLY lower > into cold tap water to cool. > 16. When completely cold and solid, use a heavy knife to score and trim > the wax blocks. > 17. To section, melt the trimmed block onto the block holder. > > Bryan Llewellyn > > > ----- Original Message ----- From: "Merced Leiker" > To: > Sent: Tuesday, September 09, 2008 1:54 PM > Subject: [Histonet] Manual Paraffin Embedding > > >> Does anyone process and embed tissues manually instead of using >> automated and expensive equipment? Can you tell me how you do it? >> Thanks. >> >> Merced >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 In order to put yourself in someone else's shoes, you must first take off yours. From rjbuesa <@t> yahoo.com Wed Sep 10 14:17:02 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 10 14:17:06 2008 Subject: [Histonet] Recyclers In-Reply-To: Message-ID: <468104.15344.qm@web65703.mail.ac4.yahoo.com> The recycler I used was a BR Instruments, a real workhorse. Ren? J. --- On Wed, 9/10/08, Bauer, Karen wrote: From: Bauer, Karen Subject: [Histonet] Recyclers To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 10, 2008, 12:58 PM Hello, I'm in the market to demo some recyclers. Any vendors are welcome to contact me personally. I've already contacted CBG and BR Instruments, so vendors from those areas need not reply to this message. Anyone on Histonet that would like to give me their likes and dislikes of certain recyclers, I'm all ears. Thanks much, Karen Karen L. Bauer HT(ASCP) Department of Pathology Histology Section Chief Luther Hospital Eau Claire, WI 715-838-3205 ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gedinite <@t> gmail.com Wed Sep 10 15:38:26 2008 From: gedinite <@t> gmail.com (Richard Nelson) Date: Wed Sep 10 15:38:31 2008 Subject: [Histonet] TBS Cassette labeler Message-ID: Hello: I am familiar with the TBS cassette labeler and would be happy to answer any questions about it. It is a work horse and can be adapted for many uses including bar codes. Rick gedinite@gmail.com From gedinite <@t> gmail.com Wed Sep 10 15:43:13 2008 From: gedinite <@t> gmail.com (Richard Nelson) Date: Wed Sep 10 15:43:16 2008 Subject: [Histonet] HM 550 Cryostat Message-ID: Hello: I have an HM 550 Cryostat if anyone is interested. Rick gedinite@gmail.com From scoop <@t> mail.nih.gov Wed Sep 10 16:15:27 2008 From: scoop <@t> mail.nih.gov (Sharon) Date: Wed Sep 10 16:15:34 2008 Subject: [Histonet] does anyone know what the bone marrow looks like in aceruloplasminemia or does anyone have an appropriate reference? Message-ID: Dear All, Does anyone know a good reference for appearance of the bone marrow biopsy in aceruloplasminemia? I know I should be able to look it up but I can't find it. Thanks, Sharon From leiker <@t> buffalo.edu Wed Sep 10 16:24:37 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Wed Sep 10 16:24:42 2008 Subject: [Histonet] Manual Paraffin Embedding In-Reply-To: <8EEF817B88BE45B668DB4F25@bchwxp2702.ad.med.buffalo.edu> References: <6089D76726392CD3B3399BFC@bchwxp2702.ad.med.buffalo.edu> <9A9BD3D0B0284D23904E676EA082BD4F@Compaq> <8EEF817B88BE45B668DB4F25@bchwxp2702.ad.med.buffalo.edu> Message-ID: Bryan, Just one question on applying the vacuum: Do you keep the melted paraffin at 65 while applying it, or slightly higher to make sure the wax doesn't solidify on the surface? Thanks, Merced --On Wednesday, September 10, 2008 3:05 PM -0400 Merced Leiker wrote: > Thanks for all the detail, Bryan! We will try this and see how it works. > Thanks to everyone else who replied as well, your tips are taken into > consideration, too. > > Merced > > --On Tuesday, September 09, 2008 3:21 PM -0700 Bryan Llewellyn > wrote: > >> I don't do this anymore, nor for 40 years now, but this is what we used >> to do aeons ago. >> >> 1. Fix in 10% NBF for 48 hours. >> 2 Rinse off excess with tap water for 1 minute. >> 3. Select pieces of tissue with maximum dimensions of 2cm x 1.5 cm x 0.3 >> cm. >> 4. Place into cassettes if you have them, then into a large container. >> If you do not have cassettes, place into small jars, each case in a >> different jar. Place a label in each cassette or each jar with the case >> ID. >> 5. Cover the tissue or cassettes with 70% ethanol, methylated spirits or >> isopropanol, agitate gently. Leave overnight. >> 6. Next morning, replace the 70& alcohol with 85% alcohol, leave for the >> day, agitating gently periodically. >> 7. Before leaving in the evening, replace the alcohol with 95% alcohol, >> agitate gently and leave overnight. >> 8. Next morning, replace the alcohol with 100% alcohol, gently agitate >> periodically. Repeat at noon and before you 9. leave for the evening, >> gently agitating. >> 10. Next morning, replace the alcohol with clearant, preferably xylene or >> toluene. Leave for one hour, gently agitating periodically. >> 11. Repeat the clearant twice more, agitate gently. >> 12. Place into premelted paraffin wax for 1 hour at 65C. Check >> periodically and when all congealed wax has remelted, agitate gently. >> 13. Repeat at least twice more, preferably under vacuum - not too strong. >> Some technologists used to leave the final change overnight. Doing so >> doesn't do much harm and improves penetration. Agitation can't be done >> under vacuum, so release the vacuum periodically, agitate and reapply it. >> 14. Block out into molds. (If you do not have molds, use a metal - >> tinned steel or aluminum - lid with a depth of 1cm. LIGHTLY coat it with >> glycerol first.) Place a thin (3mm) layer of hot wax in the mold and >> place the tissue, with the surface to be sectioned down, into it. Top up >> the mold with wax so there is at least 2-3 mm wax over the top of the >> tissue. Put the ID label conspiciously next to the tissue. Do NOT block >> out more than one tissue or case without placing the ID labels, this WILL >> lead to serious identification errors. Do all this by the oven and keep >> the door closed as much as possible. Work fast so that the wax around >> the tissues does not begin to congeal as that causes problems during >> sectioning and floating out. You must get the wax around the tissue and >> the wax in the mold to blend completely, so if the wax congeals around >> the tissue, leave it to remelt before blocking out. Many of us used to >> keep a bunsen burner alight and flame the top of the molds to keep the >> wax molten - a practice probably considered unsafe now. >> 15. Allow the wax in the mold to skin over thoroughly, then GENTLY lower >> into cold tap water to cool. >> 16. When completely cold and solid, use a heavy knife to score and trim >> the wax blocks. >> 17. To section, melt the trimmed block onto the block holder. >> >> Bryan Llewellyn >> >> >> ----- Original Message ----- From: "Merced Leiker" >> To: >> Sent: Tuesday, September 09, 2008 1:54 PM >> Subject: [Histonet] Manual Paraffin Embedding >> >> >>> Does anyone process and embed tissues manually instead of using >>> automated and expensive equipment? Can you tell me how you do it? >>> Thanks. >>> >>> Merced >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > 354 BRB (Lee Lab) / 140 Farber Hall (mail) > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > In order to put yourself in someone else's shoes, > you must first take off yours. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 In order to put yourself in someone else's shoes, you must first take off yours. From amosbrooks <@t> gmail.com Wed Sep 10 18:50:18 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Sep 10 18:50:23 2008 Subject: [Histonet] Manual Paraffin Embedding Message-ID: <582736990809101650v60de0120je0192535876626d6@mail.gmail.com> Hi, I have taken to manually processing mouse hearts for a particularly finicky researcher. I do it rather like a special stain. I set the cassettes in a covered jar and set a timer. Change solutions when the timer goes off. Infiltration is tricky with out a vacuum, but it can be done it just takes longer. Embedding can be done with a paraffin dispenser as has been mentioned. You can however do it without embedding molds if you have more time than money. You can make little boxes from aluminum foil by folding the edges. There is a nice description of how to do this in Gretchen Humanson's Animal Tissue Techniques. Just think Old Skool histology. (BTW to Rene: Great book recommendation! I'm lovin' it!) Good Luck, Amos Message: 1 Date: Tue, 09 Sep 2008 16:54:43 -0400 From: Merced Leiker Subject: [Histonet] Manual Paraffin Embedding To: histonet@lists.utsouthwestern.edu Message-ID: <6089D76726392CD3B3399BFC@bchwxp2702.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed Does anyone process and embed tissues manually instead of using automated and expensive equipment? Can you tell me how you do it? Thanks. Merced From joost.bruijntjes <@t> tno.nl Thu Sep 11 05:04:13 2008 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Thu Sep 11 05:05:19 2008 Subject: [Histonet] BrdU Message-ID: <8865601DD17A554CB489C17FFD8A51B20193B18A@MAIL04.tsn.tno.nl> Hi Histonetters I do have a problem with BrdU, and I hope someone of you can give a kind of solution/explanation. I work with NALT (Nose Associated Lymphoid Tissue) of both rats and mice, which were fixed in a fixative composed of acetic acid, formaldehyde, ethanol and aqua dest. After 48 hours this fixative was replaced by ethanol. Later on the tissues were dehydrated and embedded in paraffin. Just because the lymphoid tissues material is so small, about 10 paraffin slides were collected. I started with the rat NALT's. One paraffin slide was stained with HE, and a few other slides were stained with a monoclonal antibody directed against BrdU. I was satisfied with the staining, so far no problem. After the rats, the same story with the mice NALT's. But I did not get any positive staining. Some days later I repeated the first try-out included with some positive controls from the rat-study with the primary antibody I used in the first part, and the biotin conjugated primary antibody as well, but again no positive staining. The pre-treatment of the slides for rat and mice is the same. The only difference lies in the primary and secondary antibody. For rat tissue I use a non-conjugated monoclonal antibody, followed by HRP-conjugated powervision. For mice tissue I use a biotin conjugated monoclonal antibody, followed by a HRP-conjugated streptavidin. Can anyone give me an explanation? The storage of the slides was in a room with an equal temperature (about 20-21?C) and humidity. Is it possible that the epitopes in the single slides are destroyed so that they are not recognizable anymore by the antibody? The time between preparing the slides and the BrdU staining of the rats NALT slides was at the most 4 weeks, while the first negative staining on the mice NALT's appeared after about 8 weeks. Thanks in advance Joost Bruijntjes TNO Quality of Life Zeist The Netherlands TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From dellav <@t> musc.edu Thu Sep 11 08:31:34 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Sep 11 08:31:42 2008 Subject: [Histonet] NSH Blog In-Reply-To: References: Message-ID: I didn't see this announcement posted on the list and thought it might be of interest to some. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 FYI BLOGGING NSH -------------------------------------------------------------------------------- The National Society for Histotechnology's Annual Symposium/Convention is one of the preeminent events for histology and pathology laboratory administrators and professionals. Each year it continues to grow in popularity and attendance as NSH members and people from all facets of the histotechnology industry come together to share ideas and get a first-hand look at the new and exciting technologies that are helping labs work smarter and become more effective. This year it's being held at the David Lawerence Convention Center in Pittsburgh, PA on Sept 12 - 18. General Data will be showing our ID/Positive line of bar code specimen identification solutions (we're at booth 506 & 508). If you're coming to the show, please stop by our booth and say hi. If you can't make it to the event, stay tuned to this blog. In addition to exhibiting, we'll be blogging the show. During (and after) the show, we'll be posting pictures, video interviews, and other information on what we find at the NSH Convention - what's new, what's hot, what's on attendees' minds. Our intention is for this blog to be the eyes and ears at NSH for the entire histo community, particularly for those who can't attend the event. Please pass this on to anyone who you feel might be interested in following our posts from the NSH Convention. And if you have any comments or suggestions regarding topics or other subject matter from NSH you would like us to cover, just let us know by e-mail or in the comments section. Tags : posted By : Ralph Moher on 9/8/2008 12:31:18 PM in http://www.general-data.com/Blogs/post.aspx?id=35 From MVaughan4 <@t> ucok.edu Thu Sep 11 09:07:22 2008 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Thu Sep 11 09:07:32 2008 Subject: [Histonet] Antigen retrieval 'tween night and next day In-Reply-To: Message-ID: Brian, I haven't seen your procedure for antigen retrieval before. I usually see heat/citrate buffer or some kind of protease treatment. It seems pretty straightforward. Have you ever used this procedure for keratins or basement membrane proteins? Brian wrote: I have performed antigen retrieval by leaving slides overnight in the refrigerator immersed in PBS + 0.05% Tween 20. I'm sure there are many other ways to accomplish antigen retrieval, heat merely accelerates the process. Thanks. Mel Melville B. Vaughan, Ph. D. Assistant Professor of Biology Campus Coordinator, NSF Sure-Step program University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 Telephone 405-974-5725 Fax 405-974-5726 ----------------------------------------- **CONFIDENTIALITY** -This email (including any attachments) may contain confidential, proprietary and privileged information. Any unauthorized disclosure or use of this information is prohibited. From David.Burk <@t> pbrc.edu Thu Sep 11 09:25:51 2008 From: David.Burk <@t> pbrc.edu (David Burk) Date: Thu Sep 11 09:31:52 2008 Subject: [Histonet] Quantifying lipid in rat liver Message-ID: <6749EC8B7FC98943A6C22C4D16BF79460153F78F@pbrcas30.pbrc.edu> Oh esteemed experts who have no peer, I am in need of advice on how to quickly and easily quantify lipid content in rat liver. I believe a traditional approach is to process/embed in paraffin/section/H&E and then look for the holes left by the lipid droplets. While this is certainly doable it does require that your samples 'look nice'. Unfortunately, many of the sections we have seen here are kind of banged up and morphology is poor. I was wondering if you all have another method that you prefer for this type of analysis. For example, can I just cryosection and stain with Bodipy or Oil Red-O? In those cases I could use the fluorescent nature of the probe to quantify fat content very quickly on a computer. Here, damage caused during sectioning should not create too many problems in quantification as the stain is specific for lipid. If we wished to avoid image analysis altogether, could we just drop a rat liver in an NMR and get content that way? Thanks so much for all your help! David Burk Pennington Biomedical Research Center Baton Rouge, LA 70808 From dlschneider <@t> gmail.com Thu Sep 11 10:40:21 2008 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Thu Sep 11 10:40:29 2008 Subject: [Histonet] Protocol: Saving unstained slides... Message-ID: <1085e7000809110840k4b370fc5ld3b7e5be3f738d36@mail.gmail.com> I thought I'd poll the histonet community on an issue we're facing: On needle biopsies for tumor, what, if any, is your protocol for making unstained slides "on the front end" for potential use with immunostains later (so that the specimen isn't exhausted producing levels x 3, and you have good sections of tumor for immunostains)? Is this protocol followed on all needle biopsies for tumor? And what happens to any unused, unstained slides? Are they discarded (if so, after how long?) or kept indefinitely? Thanks for your input! Daniel Schneider From jqb7 <@t> cdc.gov Thu Sep 11 11:09:03 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Sep 11 11:09:32 2008 Subject: [Histonet] test: please delete Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C1BD@LTA3VS011.ees.hhs.gov> From gu.lang <@t> gmx.at Thu Sep 11 12:53:11 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Sep 11 12:53:35 2008 Subject: [Histonet] formalin-formula and osmolarity Message-ID: <4C83F5D6B69040FDAA5FD44BF65A3CA5@dielangs.at> Hi all, I need the help of those with a talent in chemical calculation. This formula is used in our institute since 1970, but nobody knows the origin or a publication. Formalin-formula: 8% buffered Formaldehyd 2 Liter 36% Formaldehyd 2 Liter Phosphatpuffer pH 7,8 6 Liter Aqua dest. EndpH 7,4 commercial Phosphatpuffer contents: Natrium di-hydrogenphosphat monohydrat 1,810 g di-Natrium monohydrogenphosphat dihydrat 3,756 g Natriumchlorid 4,4 g Water ad 1000 ml Phosphatebuffer has a molarity of 34,2 mmol/l. Can anybody calculate the osmolarity of the ready formalin-solution for me? What could be the advantages or disadvantages compared to Lillies 4% NBF or Carson's 4% NBF? Is the buffer-effect big enough in comparison to other formulas with 0,1 M Phosphatebuffer? Pleas help, any input is appreciated. Gudrun Lang Linz, Austria . From gvdobbin <@t> ihis.org Thu Sep 11 13:02:09 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Sep 11 13:02:40 2008 Subject: [Histonet] Protocol: Saving unstained slides... Message-ID: Hi Daniel, All of our "blanks" are saved for a short time (ie 2-4 weeks) just in case, but those from cases that have very little tissue left in the block or small malignant foci are filed with the H&E slides (and any other stained slides of course) that go with the case. The idea is that if in 2 years we want to restain the tumour for something new or (heaven forbid!) a re-check, the pathologist would generally be asking to see the original H&E's anyway; and at that time we would discover that indeed blanks do exist on the case already. Hope this helps (Disclaimer-I'm in Canada and none of this response necessarily addresses either CAP or CLIA regulations). Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "Success is not the key to happiness. Happiness is the key to success. If you love what you are doing, you will be successful." - Albert Schweitzer Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "Success is not the key to happiness. Happiness is the key to success. If you love what you are doing, you will be successful." - Albert Schweitzer >>> "Daniel Schneider" 9/11/2008 12:40 PM >>> I thought I'd poll the histonet community on an issue we're facing: On needle biopsies for tumor, what, if any, is your protocol for making unstained slides "on the front end" for potential use with immunostains later (so that the specimen isn't exhausted producing levels x 3, and you have good sections of tumor for immunostains)? Is this protocol followed on all needle biopsies for tumor? And what happens to any unused, unstained slides? Are they discarded (if so, after how long?) or kept indefinitely? Thanks for your input! Daniel Schneider _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From MVaughan4 <@t> ucok.edu Thu Sep 11 13:27:29 2008 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Thu Sep 11 13:27:39 2008 Subject: [Histonet] Unstained slide storage In-Reply-To: Message-ID: Daniel, I have found that saving slides with FFPE skin sections 5-10 micrometer thick, the antigenicity declines with age, even when stored in the fridge. I have tested slides from the same block freshly cut versus 2-3 years stored on a microscope slide in the fridge. Whether this applies to needle biopsies of tumor tissue I don't know...now I just section what I need and return the block to the fridge. Mel Melville B. Vaughan, Ph. D. Assistant Professor of Biology Campus Coordinator, NSF Sure-Step program University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 phone 405-974-5725 Message: 15 Date: Thu, 11 Sep 2008 10:40:21 -0500 From: "Daniel Schneider" Subject: [Histonet] Protocol: Saving unstained slides... To: histonet@lists.utsouthwestern.edu Message-ID: <1085e7000809110840k4b370fc5ld3b7e5be3f738d36@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I thought I'd poll the histonet community on an issue we're facing: On needle biopsies for tumor, what, if any, is your protocol for making unstained slides "on the front end" for potential use with immunostains later (so that the specimen isn't exhausted producing levels x 3, and you have good sections of tumor for immunostains)? Is this protocol followed on all needle biopsies for tumor? And what happens to any unused, unstained slides? Are they discarded (if so, after how long?) or kept indefinitely? Thanks for your input! Daniel Schneider ----------------------------------------- **CONFIDENTIALITY** -This email (including any attachments) may contain confidential, proprietary and privileged information. Any unauthorized disclosure or use of this information is prohibited. From Alistair.Lindsay <@t> btinternet.com Thu Sep 11 13:42:44 2008 From: Alistair.Lindsay <@t> btinternet.com (Alistair Lindsay) Date: Thu Sep 11 13:43:14 2008 Subject: [Histonet] Pig endothelium Message-ID: Dear All, Does anyone have any experience with staining pig endothelium, specifically keeping rings of blood vessels alive? I am collecting fresh tissue from the slaughterhouse and incubating it with tumour necrosis factor to induce VCAM-1 expression. However, I suspect that the tissue quickly loses viability and this is why I can?t visualise any VCAM with IHC. Does anyone have any tips for keeping the rings of tissue going/fixing/antigen retrieval? Many thanks Alistair Lindsay MBChB MRCP Tel.: 07989404808 Alistair.Lindsay@btinternet.com Alistair.Lindsay@cardiov.ox.ac.uk From JWeems <@t> sjha.org Thu Sep 11 13:44:19 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Sep 11 13:44:35 2008 Subject: [Histonet] Pig endothelium In-Reply-To: References: Message-ID: <982A0A9461F9BF438C7B19A6E425A383600C3E@ITSSSXM01V6.one.ads.che.org> Lipstick, maybe? Sorry, couldn't help it... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alistair Lindsay Sent: Thursday, September 11, 2008 2:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pig endothelium Dear All, Does anyone have any experience with staining pig endothelium, specifically keeping rings of blood vessels alive? I am collecting fresh tissue from the slaughterhouse and incubating it with tumour necrosis factor to induce VCAM-1 expression. However, I suspect that the tissue quickly loses viability and this is why I can?t visualise any VCAM with IHC. Does anyone have any tips for keeping the rings of tissue going/fixing/antigen retrieval? Many thanks Alistair Lindsay MBChB MRCP Tel.: 07989404808 Alistair.Lindsay@btinternet.com Alistair.Lindsay@cardiov.ox.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From rjbuesa <@t> yahoo.com Thu Sep 11 14:48:29 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 11 14:48:38 2008 Subject: [Histonet] Unstained slide storage In-Reply-To: Message-ID: <579701.32831.qm@web65715.mail.ac4.yahoo.com> Decreased antigenicity applies to any section stored and unprotected from oxygen (air) oxidation. Ren? J. --- On Thu, 9/11/08, MVaughan4@ucok.edu wrote: From: MVaughan4@ucok.edu Subject: [Histonet] Unstained slide storage To: histonet@lists.utsouthwestern.edu Date: Thursday, September 11, 2008, 2:27 PM Daniel, I have found that saving slides with FFPE skin sections 5-10 micrometer thick, the antigenicity declines with age, even when stored in the fridge. I have tested slides from the same block freshly cut versus 2-3 years stored on a microscope slide in the fridge. Whether this applies to needle biopsies of tumor tissue I don't know...now I just section what I need and return the block to the fridge. Mel Melville B. Vaughan, Ph. D. Assistant Professor of Biology Campus Coordinator, NSF Sure-Step program University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 phone 405-974-5725 Message: 15 Date: Thu, 11 Sep 2008 10:40:21 -0500 From: "Daniel Schneider" Subject: [Histonet] Protocol: Saving unstained slides... To: histonet@lists.utsouthwestern.edu Message-ID: <1085e7000809110840k4b370fc5ld3b7e5be3f738d36@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I thought I'd poll the histonet community on an issue we're facing: On needle biopsies for tumor, what, if any, is your protocol for making unstained slides "on the front end" for potential use with immunostains later (so that the specimen isn't exhausted producing levels x 3, and you have good sections of tumor for immunostains)? Is this protocol followed on all needle biopsies for tumor? And what happens to any unused, unstained slides? Are they discarded (if so, after how long?) or kept indefinitely? Thanks for your input! Daniel Schneider ----------------------------------------- **CONFIDENTIALITY** -This email (including any attachments) may contain confidential, proprietary and privileged information. Any unauthorized disclosure or use of this information is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagnone <@t> KGH.KARI.NET Thu Sep 11 14:48:40 2008 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Thu Sep 11 14:48:56 2008 Subject: [Histonet] Saving unstained slides Message-ID: We pick up 10 sections on any needle/core biopsy of suspected tumour/lymphoma. Usually if immunostains are ordered, the request comes the same day our routine slides go out, or possibly the next day. Cutting them "up front" seems the prudent way to go if there is any risk of the biopsy being cut away during subsequent sectioning for immunostains. How about 1 stained and 3-5 unstained at each level? This has saved us precious tissue many times, especially when multiple markers are requested. If more markers are requested than there are unstained sections, the pathologist will priorize his request and we then cut more slides, trying to conserve as much tissue as possible. As for retaining these unstained slides, they're definitely worth keeping until after the dx. is made, after that it's up to you how long you want to retain them in your protocol. Antigenicity and storage capacity/cost are among issues to be considered. As Greg mentioned, this is from a Canadian viewpoint. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From marktarango <@t> gmail.com Thu Sep 11 14:50:44 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Sep 11 14:50:54 2008 Subject: [Histonet] Pig endothelium In-Reply-To: References: Message-ID: <5b6eb13e0809111250x106f7e2fnd66ea80266188035@mail.gmail.com> Are you putting the tissue into media like RPMI or DMEM to keep it viable until you use it? P.S. Joyce, I loved the lipstick comment hehe On Thu, Sep 11, 2008 at 11:42 AM, Alistair Lindsay < Alistair.Lindsay@btinternet.com> wrote: > Dear All, > > Does anyone have any experience with staining pig endothelium, specifically > keeping rings of blood vessels alive? > > I am collecting fresh tissue from the slaughterhouse and incubating it with > tumour necrosis factor to induce VCAM-1 expression. However, I suspect > that > the tissue quickly loses viability and this is why I can?t visualise any > VCAM with IHC. > > Does anyone have any tips for keeping the rings of tissue > going/fixing/antigen retrieval? > > Many thanks > > Alistair Lindsay MBChB MRCP > > Tel.: 07989404808 > > Alistair.Lindsay@btinternet.com > Alistair.Lindsay@cardiov.ox.ac.uk > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MDiCarlo <@t> KaleidaHealth.Org Thu Sep 11 15:04:39 2008 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Thu Sep 11 15:04:51 2008 Subject: [Histonet] bottom of water bath Message-ID: <1B73766A27A1554CB2729B6432E81301E5D648@KALEXMB04.KaleidaHealth.org> Hello Histonetters, Working in a Bone Lab, I cut large sections which often require using 5 X 7 inch slides. I finally found a new water bath which is deep and wide enough for my lab needs. Unfortunately, the inside of the water bath is stainless steel which means silver in color. Since it can not be painted, does anyone have any suggestions as to what I can do or use to make the bottom of the water bath black so I can see the tissue easier? I would appreciate your help. Thank you. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From KKay <@t> chr.ab.ca Thu Sep 11 15:08:26 2008 From: KKay <@t> chr.ab.ca (Kay, Karen) Date: Thu Sep 11 15:11:15 2008 Subject: [Histonet] RE: Histonet Digest, Vol 58, Issue 13 - RECYCLERS In-Reply-To: Message-ID: <9C0BD812BAB0BA4DB7E7FD4F5FA3CAA80A494CA7@exbe.chr.ab.ca> Hello, We have used the CBG product for a few years now and are very happy with both the equipment and support/service. The recycler is simple to use. We have been able to fit it into the "tasks" of the day. It has been a very reliable instrument. The staff are always willing to help whenever we have called them. Karen J Kay, MLT Pathology Supervisor Laboratory Chinook Regional Hospital Lethbridge, Alberta, Canada ************************************************************************ Message: 13 Date: Wed, 10 Sep 2008 11:58:09 -0500 From: "Bauer, Karen" Subject: [Histonet] Recyclers To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello, I'm in the market to demo some recyclers. Any vendors are welcome to contact me personally. I've already contacted CBG and BR Instruments, so vendors from those areas need not reply to this message. Anyone on Histonet that would like to give me their likes and dislikes of certain recyclers, I'm all ears. Thanks much, Karen Karen L. Bauer HT(ASCP) Department of Pathology Histology Section Chief Luther Hospital Eau Claire, WI 715-838-3205 This communication is intended for the use of the recipient to which it is addressed, and may contain confidential, personal and or privileged information. Please contact us immediately if you are not the intended recipient. Do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. From mcauliff <@t> umdnj.edu Thu Sep 11 15:18:11 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Sep 11 15:17:52 2008 Subject: [Histonet] bottom of water bath In-Reply-To: <1B73766A27A1554CB2729B6432E81301E5D648@KALEXMB04.KaleidaHealth.org> References: <1B73766A27A1554CB2729B6432E81301E5D648@KALEXMB04.KaleidaHealth.org> Message-ID: <48C97D03.3020002@umdnj.edu> You can paint stainless steel if you prepare the surface and prime correctly. Call the help line of Benjamin Moore, Sherman-Williams, Zinsser or ?? and tell them what you need. Geoff DiCarlo, Margaret wrote: > Hello Histonetters, > > > > Working in a Bone Lab, I cut large sections which often require using 5 > X 7 inch slides. I finally found a new water bath which is deep and > wide enough for my lab needs. Unfortunately, the inside of the water > bath is stainless steel which means silver in color. Since it can not > be painted, does anyone have any suggestions as to what I can do or use > to make the bottom of the water bath black so I can see the tissue > easier? I would appreciate your help. > > > > Thank you. > > Peggy DiCarlo HT (ASCP) > > Orthopedic Bone Lab > > Buffalo General Hospital > > 100 High St. > > Buffalo, NY 14203 > > 716-859-1293 > > > > > > 2007 Best Places to Work Finalist > Visit our careers page at www.kaleidahealth.org/careers > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From pmarcum <@t> vet.upenn.edu Thu Sep 11 15:25:15 2008 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Sep 11 15:25:24 2008 Subject: [Histonet] bottom of water bath In-Reply-To: <48C97D03.3020002@umdnj.edu> References: <1B73766A27A1554CB2729B6432E81301E5D648@KALEXMB04.KaleidaHealth.org> <48C97D03.3020002@umdnj.edu> Message-ID: <000001c9144c$80490970$095a5b82@vet.upenn.edu> Margaret, Look for a silicone microwave oven mat and cut it to size. They come in colours and should lay nicely on the bottom. If need be it could be weighted down along the sides with fishing weights or even a small metal bar cut to size. It is easier than painting and makes cleaning a breeze as it lifts out and rinse clean when the water bath is emptied. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Thursday, September 11, 2008 4:18 PM To: DiCarlo, Margaret Cc: histonet@pathology.swmed.edu Subject: Re: [Histonet] bottom of water bath You can paint stainless steel if you prepare the surface and prime correctly. Call the help line of Benjamin Moore, Sherman-Williams, Zinsser or ?? and tell them what you need. Geoff DiCarlo, Margaret wrote: > Hello Histonetters, > > > > Working in a Bone Lab, I cut large sections which often require using 5 > X 7 inch slides. I finally found a new water bath which is deep and > wide enough for my lab needs. Unfortunately, the inside of the water > bath is stainless steel which means silver in color. Since it can not > be painted, does anyone have any suggestions as to what I can do or use > to make the bottom of the water bath black so I can see the tissue > easier? I would appreciate your help. > > > > Thank you. > > Peggy DiCarlo HT (ASCP) > > Orthopedic Bone Lab > > Buffalo General Hospital > > 100 High St. > > Buffalo, NY 14203 > > 716-859-1293 > > > > > > 2007 Best Places to Work Finalist > Visit our careers page at www.kaleidahealth.org/careers > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jwatson <@t> gnf.org Thu Sep 11 17:29:37 2008 From: jwatson <@t> gnf.org (James Watson) Date: Thu Sep 11 17:29:42 2008 Subject: [Histonet] San Diego Chapter of CSH field trip Message-ID: The San Diego chapter of the Ca. Society of Histotechnology is sponsoring a tour of all the labs at the Beckman Research Center (Genetics and Microdiagnostic labs) adjacent to the Wild Animal Park in Escondido: Thursday September 25, 2005 2p.m. This tour is at no cost and should last about an hour. This tour will not involve admission to the Wild Animal Park. So if you want to go to Wild Animal Park you may do that on your own. This trip is open to members of the chapter and other interested adults. We can accommodate no more than thirty people. Make you reservation early so you will not miss out on this. Parking is limited so carpool if you can. We will need to know how many cars are going so when you RSVP let Kris know. Traveling east on 78, go past the main entrance and then make the next left. There is a closed gate. To gain excess, press the button on the keypad and identify yourself. http://maps.google.com/maps?f=q&hl=en&geocode=&q=Wild+animal+park,+escon dido,+ca&ie=UTF8&ll=33.086076,-117.039928&spn=0.064578,0.109863&z=13&iwl oc=A The cut off date and time for reservations are at 4pm Monday September 22, 2008. R.S.V.P. cwright@gnf.org If you are interested in becoming a member of the San Diego chapter, please contact me. James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org From AnthonyH <@t> chw.edu.au Thu Sep 11 17:33:18 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Sep 11 17:33:32 2008 Subject: [Histonet] Quantifying lipid in rat liver In-Reply-To: <6749EC8B7FC98943A6C22C4D16BF79460153F78F@pbrcas30.pbrc.edu> Message-ID: You could try this: Linoleic Acid Method for the demonstration of Lipids in Paraffin Sections Tracy & Walia (2002) have described an ingenious method to fix lipids for staining fat embolism in paraffin sections: (Tracy, R.E., Walia, P., (2002) "A Method to fix lipids for staining fat embolism in paraffin sections" Histopath 41:75-79) Solutions: 1. Saturated solution of linoleic acid in 70% ethylene glycol To 500ml of 70% ethylene glycol, add 5g linoleic acid and 2g lecithin, mix for one hour and stand for several hours. Draw off the lower phase in a separatory funnel 2. 2% chromic acid 3. 70 % ethanol 4. 5% Sodium bicarbonate Method: 1. Place 2mm thick sections in solution 1 for 3 days at 56oC 2. Rinse for 8 hours in 70% ethanol 3. Rinse in water for 8 hours 4. Immerse in 2% chromic acid for 24 hours at 4oC. 5. Rinse in water for 24 hours 6. Place in 5% sodium bicarbonate for 24 hours 7. Rinse in water 8 hours 8. Process from 70% ethanol via ethanol and xylene to wax. 9. Cut paraffin sections and stain with the usual fat stains (eg oil red O) and mount in an aqueous mountant. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Burk Sent: Friday, 12 September 2008 12:26 AM To: histonet@lists.utsouthwestern.edu; Microscopy@microscopy.com Subject: [Histonet] Quantifying lipid in rat liver Oh esteemed experts who have no peer, I am in need of advice on how to quickly and easily quantify lipid content in rat liver. I believe a traditional approach is to process/embed in paraffin/section/H&E and then look for the holes left by the lipid droplets. While this is certainly doable it does require that your samples 'look nice'. Unfortunately, many of the sections we have seen here are kind of banged up and morphology is poor. I was wondering if you all have another method that you prefer for this type of analysis. For example, can I just cryosection and stain with Bodipy or Oil Red-O? In those cases I could use the fluorescent nature of the probe to quantify fat content very quickly on a computer. Here, damage caused during sectioning should not create too many problems in quantification as the stain is specific for lipid. If we wished to avoid image analysis altogether, could we just drop a rat liver in an NMR and get content that way? Thanks so much for all your help! David Burk Pennington Biomedical Research Center Baton Rouge, LA 70808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From dw18 <@t> uchicago.edu Thu Sep 11 19:16:59 2008 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Thu Sep 11 19:17:07 2008 Subject: [Histonet] Re: BrdU Message-ID: <20080911191659.BEB59005@m4500-03.uchicago.edu> Hi Joost & the Histonet I have some general comments on BrdU that i hope help. Maybe someone else has more specific ideas relating to your tissue too? It seems you are having problems with 2 things at once: a) switching species b) switching antibodies My guess is you only have control over the latter but either could be the problem! a) species - since DNA is DNA, you don't have to worry about species specificity of your antibody. Unless you are doing tissue culture, it's not however always simple to get the same BrdU incorporation in different species. I take it that someone else - possibly different people - labelled the mice and rats and also that it was done in vivo. Even repeat experiments on the same species are variable. The BrdU doesn't always go into solution equally (it needs a dilute base, not buffered saline) and it has to have a phosphate added on inside the cell before it is incorporated. So metabolic rate is important not just dose/kg. It's a good idea to collect control tissue from every animal to confirm incorporation - pick any abundant, proliferating tissue. I've often used gut. You then also have lots of material to work up and compare different antibodies. I'm probably not the only one who'll say you have to try your 2 antibodies side by side on the same specimen. b) Different antibodies. Hmmm. You mention fixation procedures but don't say much about what you did to recover antigenicity in your paraffin sections before staining. With BrdU you have to do something just to make it accessible. In Eukaryotes, (BrdU-containing) nuclear DNA is highly compacted into nucleosomes with lots of histones and other proteins all around it and then squashed down a whole lot more. Fixation will lock all these proteins together and make it hard for the Ab to find its target. I think it's a truism that all nuclear antigens, including proteins, need some kind of harsh treatment (heat, strong acid or base, proteinase) to make the nucleus accessible. It's interesting that your unconjugated MAb worked but not the larger conjugated one - maybe it's just penetration. On this point, you didn't say whether your protocol was exactly the same for each Ab. I've used a bunch of different anti-BrdU Abs and the manufacturers have quite different recommendations about pretreatment - 2N HCl, formamide, SSC, NaOH, proteinase. If you've only got a tiny bit of tissue, you MUST do exactly what the manufacturer says [at least the first time] even if that's different from what worked for you before with a different Ab (I'd do both!). It often helps NOT to block, so the Ab can hit the naked DNA, however exposed. [c) time. You mentioned different delays before processing. BrdU staining is one of the few things where that won't matter. As DNA doesn't degrade like proteins, antigenicity isn't lost with time.] Try Again! Since (same logic) the DNA will still be there on your slides, IF BrdU was actually incorporated (see a), there's a very high likelihood that your negative slides WILL stain with either a different antibody and/or harsher pretreatment as recommended by the manufacturer. I liked BD Bioscience's MAb (clone B44) which only needed a few minutes in 0.07N NaOH to unmask to BrdU. I think it's still available. Good luck! -David -------- David A. Wright PhD University of Chicago Section of Neurosurgery ---- Original message ---- Histonet Digest, Vol 58, Issue 13 Message: 11 Date: Thu, 11 Sep 2008 12:04:13 +0200 From: "Bruijntjes, J.P. (Joost)" Subject: [Histonet] BrdU Hi Histonetters I do have a problem with BrdU, and I hope someone of you can give a kind of solution/explanation. I work with NALT (Nose Associated Lymphoid Tissue) of both rats and mice, which were fixed in a fixative composed of acetic acid, formaldehyde, ethanol and aqua dest. After 48 hours this fixative was replaced by ethanol. Later on the tissues were dehydrated and embedded in paraffin. Just because the lymphoid tissues material is so small, about 10 paraffin slides were collected. I started with the rat NALT's. One paraffin slide was stained with HE, and a few other slides were stained with a monoclonal antibody directed against BrdU. I was satisfied with the staining, so far no problem. After the rats, the same story with the mice NALT's. But I did not get any positive staining. Some days later I repeated the first try-out included with some positive controls from the rat-study with the primary antibody I used in the first part, and the biotin conjugated primary antibody as well, but again no positive staining. The pre-treatment of the slides for rat and mice is the same. The only difference lies in the primary and secondary antibody. For rat tissue I use a non-conjugated monoclonal antibody, followed by HRP-conjugated powervision. For mice tissue I use a biotin conjugated monoclonal antibody, followed by a HRP-conjugated streptavidin. Can anyone give me an explanation? The storage of the slides was in a room with an equal temperature (about 20-21?C) and humidity. Is it possible that the epitopes in the single slides are destroyed so that they are not recognizable anymore by the antibody? The time between preparing the slides and the BrdU staining of the rats NALT slides was at the most 4 weeks, while the first negative staining on the mice NALT's appeared after about 8 weeks. Thanks in advance Joost Bruijntjes TNO Quality of Life Zeist The Netherlands TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl David A. Wright, Ph.D. University of Chicago Section of Neurosurgery, MC3026 5841 S. Maryland Ave Chicago, IL 60637 USA [W] 773-834-1485 (Office) SBRI Rm J328 773-834-0395 (Lab) SBRI Rm J338 [H] 773-643-6976 Pager (@773-753-1880) #9776 Text page 120 char E-mail 7738456834@myairmail.com or web: http://www.myairmail.com to 7738456834 FAX (Neurosurgery) 773.702.3518 Web FAX & personal voicemail: (815)642-9630 Skype: davidanthonywright ================================================ Does 2+2=5 for large values of 2? From dw18 <@t> uchicago.edu Thu Sep 11 19:33:17 2008 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Thu Sep 11 19:33:24 2008 Subject: [Histonet] Re: paraformaldehyde grade Message-ID: <20080911193317.BEB59808@m4500-03.uchicago.edu> Hi Mary, Ren?, & Histonet I agree with Ren? completely about concentration & different paraformaldehyde grades but you might want to consider a couple of other issues too: a) physical state. A coarse prill is much easier to weigh out without contamination than a fine powder. b) filtration. Before perfusing the brains you have to filter out anything that might clog the capillaries. There's usually a milky haze left after dissolving the paraformaldehyde which you have to remove. I imagine that a lower grade might have more undissolved matter, and you have to change filters a lot even with good stuff (approx every half liter). -David ------ David A. Wright PhD University of Chicago Section of Neurosurgery ---- Original message ---- >Date: Thu, 11 Sep 2008 12:09:50 -0500 (CDT) >From: histonet-request@lists.utsouthwestern.edu >Subject: Histonet Digest, Vol 58, Issue 13 Message: 1 >Date: Wed, 10 Sep 2008 10:10:42 -0700 (PDT) >From: Rene J Buesa >Subject: Re: [Histonet] Paraformaldehyde >To: histonet@lists.utsouthwestern.edu, "Mary P. Brownson" > > >It does not really matter, BUT it will have to be taken into consideration when calculating the final concentration you want to use. >Ren? J. > >--- On Tue, 9/9/08, Mary P. Brownson wrote: > >From: Mary P. Brownson >Subject: [Histonet] Paraformaldehyde >To: histonet@lists.utsouthwestern.edu >Date: Tuesday, September 9, 2008, 4:50 PM > >Hello, > >I plan to use paraformaldehyde for perfusion of rat brains. My question >is; what grade paraformaldehde should I be using? Reagent grade, 95% or >other? Or does it not matter? > >Thank you, >Mary Brownson >School of Pharmacy >University of Wyoming >Laramie, WY 82070 >mpb1211@uwyo.edu From lpwenk <@t> sbcglobal.net Fri Sep 12 04:20:29 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Sep 12 04:20:49 2008 Subject: [Histonet] Quantifying lipid in rat liver In-Reply-To: <6749EC8B7FC98943A6C22C4D16BF79460153F78F@pbrcas30.pbrc.edu> Message-ID: <000401c914b8$cd5864b0$0402a8c0@HPPav2> Could you fix the tissue in osmium tetroxide, similar to what they do in EM? - Fix first in 10% neutral buffered formalin to preserve morphology of other cells, rinse in buffer, then fix in 1% OsO4, rinse again in buffer. That would fix and stain all the fat black. - Then process the tissue as usual through alcohols and xylene (or substitute). Embed in paraffin. - Section as usual (might want to put section on charged slides). 5 um would probably work just fine. Dry slides in oven. Then, depending upon what you want, either: - deparaffinize and coverslip with synthetic mounting media - do an entire H&E on slide. Cytoplasm will look pink-brown, and nuclei will look blue-brown, but at least you can see other tissue components. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Burk Sent: Thursday, September 11, 2008 10:26 AM To: histonet@lists.utsouthwestern.edu; Microscopy@microscopy.com Subject: [Histonet] Quantifying lipid in rat liver Oh esteemed experts who have no peer, I am in need of advice on how to quickly and easily quantify lipid content in rat liver. I believe a traditional approach is to process/embed in paraffin/section/H&E and then look for the holes left by the lipid droplets. While this is certainly doable it does require that your samples 'look nice'. Unfortunately, many of the sections we have seen here are kind of banged up and morphology is poor. I was wondering if you all have another method that you prefer for this type of analysis. For example, can I just cryosection and stain with Bodipy or Oil Red-O? In those cases I could use the fluorescent nature of the probe to quantify fat content very quickly on a computer. Here, damage caused during sectioning should not create too many problems in quantification as the stain is specific for lipid. If we wished to avoid image analysis altogether, could we just drop a rat liver in an NMR and get content that way? Thanks so much for all your help! David Burk Pennington Biomedical Research Center Baton Rouge, LA 70808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MDiCarlo <@t> KaleidaHealth.Org Fri Sep 12 06:54:33 2008 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Fri Sep 12 06:54:42 2008 Subject: [Histonet] bottom of water bath In-Reply-To: <000001c9144c$80490970$095a5b82@vet.upenn.edu> References: <1B73766A27A1554CB2729B6432E81301E5D648@KALEXMB04.KaleidaHealth.org> <48C97D03.3020002@umdnj.edu> <000001c9144c$80490970$095a5b82@vet.upenn.edu> Message-ID: <1B73766A27A1554CB2729B6432E81301E5D64F@KALEXMB04.KaleidaHealth.org> Thank you everyone for all your excellent ideas for the bottom of a new stainless steel waterbath!! I can paint it after all which is what I originally planned to do. I am very grateful for this website since I work alone and have no one to ask if I have a problem, so I do appreciate all your responses. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 -----Original Message----- From: Pamela Marcum [mailto:pmarcum@vet.upenn.edu] Sent: Thursday, September 11, 2008 16:25 To: 'Geoff McAuliffe'; DiCarlo, Margaret Cc: histonet@pathology.swmed.edu Subject: RE: [Histonet] bottom of water bath Margaret, Look for a silicone microwave oven mat and cut it to size. They come in colours and should lay nicely on the bottom. If need be it could be weighted down along the sides with fishing weights or even a small metal bar cut to size. It is easier than painting and makes cleaning a breeze as it lifts out and rinse clean when the water bath is emptied. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Thursday, September 11, 2008 4:18 PM To: DiCarlo, Margaret Cc: histonet@pathology.swmed.edu Subject: Re: [Histonet] bottom of water bath You can paint stainless steel if you prepare the surface and prime correctly. Call the help line of Benjamin Moore, Sherman-Williams, Zinsser or ?? and tell them what you need. Geoff DiCarlo, Margaret wrote: > Hello Histonetters, > > > > Working in a Bone Lab, I cut large sections which often require using 5 > X 7 inch slides. I finally found a new water bath which is deep and > wide enough for my lab needs. Unfortunately, the inside of the water > bath is stainless steel which means silver in color. Since it can not > be painted, does anyone have any suggestions as to what I can do or use > to make the bottom of the water bath black so I can see the tissue > easier? I would appreciate your help. > > > > Thank you. > > Peggy DiCarlo HT (ASCP) > > Orthopedic Bone Lab > > Buffalo General Hospital > > 100 High St. > > Buffalo, NY 14203 > > 716-859-1293 > > > > > > 2007 Best Places to Work Finalist > Visit our careers page at www.kaleidahealth.org/careers > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From integrated.histo <@t> gmail.com Fri Sep 12 10:42:09 2008 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Fri Sep 12 10:42:13 2008 Subject: [Histonet] KOH for Toenails Message-ID: <5d9104a30809120842v50951003u3cde7c29c1e19af9@mail.gmail.com> Has anyone used (or seen) the KOH method of testing toenails for fungus? I am looking for a procedure as our pathologists said they want us to start using this method. Also, what are the pros and cons of this method? Cindy From rjbuesa <@t> yahoo.com Fri Sep 12 11:04:04 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 12 11:04:10 2008 Subject: [Histonet] KOH for Toenails In-Reply-To: <5d9104a30809120842v50951003u3cde7c29c1e19af9@mail.gmail.com> Message-ID: <640176.46702.qm@web65702.mail.ac4.yahoo.com> Under separate cover I am sending a review I wrote on this issue with the procedure you are looking for. Ren? J. --- On Fri, 9/12/08, Cindy DuBois wrote: From: Cindy DuBois Subject: [Histonet] KOH for Toenails To: histonet@lists.utsouthwestern.edu Date: Friday, September 12, 2008, 11:42 AM Has anyone used (or seen) the KOH method of testing toenails for fungus? I am looking for a procedure as our pathologists said they want us to start using this method. Also, what are the pros and cons of this method? Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From awatanabe <@t> tgen.org Fri Sep 12 11:50:48 2008 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Fri Sep 12 11:50:57 2008 Subject: [Histonet] Re: Unstained slide storage In-Reply-To: <20080912161208.8080CDF07@mr2.tgen.org> Message-ID: I consistently cut more slides than I need and store them in the refrigerator after I dip them in melted paraffin. I have seen decreased antigenicity over years with some antibodies but not all. Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatanabe@tgen.org www.tgen.org From ardavis <@t> wlgore.com Fri Sep 12 12:00:36 2008 From: ardavis <@t> wlgore.com (Araceli Davis) Date: Fri Sep 12 12:00:54 2008 Subject: [Histonet] Araceli Davis is out of the office will return 9/19/08 Message-ID: I will be out of the office starting 09/12/2008 and will not return until 09/19/2008. Any histology request contact Histology@WLGore.com From gedinite <@t> gmail.com Fri Sep 12 12:03:18 2008 From: gedinite <@t> gmail.com (Richard Nelson) Date: Fri Sep 12 12:03:24 2008 Subject: [Histonet] stainers Message-ID: Hello: From my experience the DRS 2000 and the Leica XL are dependable hard working units. If you have any questions contact me. Best wishes, Rick gedinite@gmail.com From Joyce.Rush <@t> sjmcmn.org Fri Sep 12 12:25:07 2008 From: Joyce.Rush <@t> sjmcmn.org (Rush, Joyce) Date: Fri Sep 12 12:26:17 2008 Subject: [Histonet] Stainers Message-ID: <2337362E8548BE4C85EE5DD5D526B0850122C93A@sjw3smail2.SJMCMN.ORG> We are a low volume lab and currently do a limited menu of special and IHC stains on a stainer. We do our H&E's by hand. The stains for our IHC stainer are getting harder and harder to get. We are considering sending our IHC's out and getting a stainer for H&E and special stains. I'd appreciate any ideas from smaller volume labs. We do about 15,000 surgicals per year. I do apologize for asking about this as I know it's been covered over the years. Thanks so much! Joyce Joyce A. Rush, BS, MT(ASCP) Director, Laboratory Services St. Joseph Medical Center 523 N. Third Street Brainerd, MN 56401 Phone: 218-828-7505 FAX: 218-828-7510 This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From NMargaryan <@t> childrensmemorial.org Fri Sep 12 13:59:14 2008 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Sep 12 14:00:23 2008 Subject: [Histonet] Unstained slide storage In-Reply-To: References: Message-ID: Hi Aprill, Why do you keep paraffin-embedded slides in refrigerator? Is it the rule? Naira -----Original Message----- Message: 1 Date: Fri, 12 Sep 2008 09:50:48 -0700 From: Aprill Watanabe Subject: [Histonet] Re: Unstained slide storage To: Message-ID: Content-Type: text/plain; charset="US-ASCII" I consistently cut more slides than I need and store them in the refrigerator after I dip them in melted paraffin. I have seen decreased antigenicity over years with some antibodies but not all. Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatanabe@tgen.org www.tgen.org From rjbuesa <@t> yahoo.com Fri Sep 12 14:24:34 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 12 14:24:41 2008 Subject: [Histonet] Stainers In-Reply-To: <2337362E8548BE4C85EE5DD5D526B0850122C93A@sjw3smail2.SJMCMN.ORG> Message-ID: <400176.92957.qm@web65705.mail.ac4.yahoo.com> I would buy a Sakura autostainer for H&E (it can also run some HC procedures), but with that work volume you will probably have about 20-25 HC per day and that amount can be handled cheaply manually. Ren? J. --- On Fri, 9/12/08, Rush, Joyce wrote: From: Rush, Joyce Subject: [Histonet] Stainers To: histonet@lists.utsouthwestern.edu Date: Friday, September 12, 2008, 1:25 PM We are a low volume lab and currently do a limited menu of special and IHC stains on a stainer. We do our H&E's by hand. The stains for our IHC stainer are getting harder and harder to get. We are considering sending our IHC's out and getting a stainer for H&E and special stains. I'd appreciate any ideas from smaller volume labs. We do about 15,000 surgicals per year. I do apologize for asking about this as I know it's been covered over the years. Thanks so much! Joyce Joyce A. Rush, BS, MT(ASCP) Director, Laboratory Services St. Joseph Medical Center 523 N. Third Street Brainerd, MN 56401 Phone: 218-828-7505 FAX: 218-828-7510 From jacobc <@t> mmc.org Fri Sep 12 14:24:49 2008 From: jacobc <@t> mmc.org (Christine Jacobs) Date: Fri Sep 12 14:25:02 2008 Subject: [Histonet] AZF fixed bone marrows and IHC's Message-ID: <20080912T152449Z_C07000110000@mmc.org> Is anyone having problems with IHC staining after a combination os AZF fixation and decal? There was a recent string on the Histonet regarding B-Plus but I can't remember exactly what was discussed. We are experiencing problems with TdT staining. We get nothing on the core biopsies and only edge artifact staining on most clots. The FFPE thymus tissue looks GREAT. We are using Cell Marque's predilute antibody on the Ventana BenchMark XT. Any and all comments will be appreciated. Chris Jacobs, HT(ASCP), QIHC Jason Wheaton, HTL (ASCP) NorDx Labs Scarborough, ME CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the use of the intended recipient(s) only and may contain information that is privileged, confidential, and prohibited from unauthorized disclosure under applicable law. If you are not the intended recipient of this message, any dissemination, distribution, or copying of this message is strictly prohibited. If you received this message in error, please notify the sender by reply email and destroy all copies of the original message and attachments. From rjbuesa <@t> yahoo.com Fri Sep 12 14:32:23 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Sep 12 14:32:27 2008 Subject: [Histonet] Unstained slide storage In-Reply-To: Message-ID: <472727.7581.qm@web65707.mail.ac4.yahoo.com> No it is not?the rule. The thing is that sections covered with paraffin will be isolated from the air and the epitopes will not be readily?oxidized. You can also keep them in a container?immersed in?mineral oil. If you do either, you do not need to keep them at low temperature. Ren? J. --- On Fri, 9/12/08, Margaryan, Naira wrote: From: Margaryan, Naira Subject: [Histonet] Unstained slide storage To: histonet@lists.utsouthwestern.edu Date: Friday, September 12, 2008, 2:59 PM Hi Aprill, Why do you keep paraffin-embedded slides in refrigerator? Is it the rule? Naira From marktarango <@t> gmail.com Fri Sep 12 14:53:31 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Sep 12 14:53:37 2008 Subject: [Histonet] AZF fixed bone marrows and IHC's In-Reply-To: <20080912T152449Z_C07000110000@mmc.org> References: <20080912T152449Z_C07000110000@mmc.org> Message-ID: <5b6eb13e0809121253m2669e0a0tae1f53d7fbc2ae21@mail.gmail.com> I had the same problem but our cores were fixed in 10% NBF. I tried everything I could think of to get it to work as far as retrieval, so I ended up having to do a real TUNEL assay. We had to assume the antibody wasn't specific enough. Same antibody and staining platform as you. Mark On Fri, Sep 12, 2008 at 12:24 PM, Christine Jacobs wrote: > Is anyone having problems with IHC staining after a combination os AZF > fixation and decal? There was a recent string on the Histonet regarding > B-Plus but I can't remember exactly what was discussed. We are experiencing > problems with TdT staining. We get nothing on the core biopsies and only > edge artifact staining on most clots. The FFPE thymus tissue looks GREAT. We > are using Cell Marque's predilute antibody on the Ventana BenchMark XT. > Any and all comments will be appreciated. > Chris Jacobs, HT(ASCP), QIHC > Jason Wheaton, HTL (ASCP) > NorDx Labs > Scarborough, ME > > CONFIDENTIALITY NOTICE: This email message, including any attachments, is > for the use of the intended recipient(s) only and may contain information > that is privileged, confidential, and prohibited from unauthorized > disclosure under applicable law. If you are not the intended recipient of > this message, any dissemination, distribution, or copying of this message is > strictly prohibited. If you received this message in error, please notify > the sender by reply email and destroy all copies of the original message and > attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jcresor <@t> lcpath.com Fri Sep 12 15:03:12 2008 From: jcresor <@t> lcpath.com (Jennifer Cresor) Date: Fri Sep 12 15:03:18 2008 Subject: [Histonet] Histotech looking for work Message-ID: <5b3c236a3a16c546aae920359ea5288b@mail2.lcpath.com> Hello all, Is anyone in need of a histotech in the Inland empire area of Southern California? I will be moving there soon. Thanks! Jennifer Cresor (360)274-5292 jcresor@lcpath.com From marktarango <@t> gmail.com Fri Sep 12 15:23:12 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Sep 12 15:23:17 2008 Subject: [Histonet] AZF fixed bone marrows and IHC's In-Reply-To: <5b6eb13e0809121253m2669e0a0tae1f53d7fbc2ae21@mail.gmail.com> References: <20080912T152449Z_C07000110000@mmc.org> <5b6eb13e0809121253m2669e0a0tae1f53d7fbc2ae21@mail.gmail.com> Message-ID: <5b6eb13e0809121323k312c4822h684bce3640100945@mail.gmail.com> Actually, i think the conclusion we reached was that the decal was the problem... On Fri, Sep 12, 2008 at 12:53 PM, Mark Tarango wrote: > I had the same problem but our cores were fixed in 10% NBF. I tried > everything I could think of to get it to work as far as retrieval, so I > ended up having to do a real TUNEL assay. We had to assume the antibody > wasn't specific enough. Same antibody and staining platform as you. > > Mark > > On Fri, Sep 12, 2008 at 12:24 PM, Christine Jacobs wrote: > >> Is anyone having problems with IHC staining after a combination os AZF >> fixation and decal? There was a recent string on the Histonet regarding >> B-Plus but I can't remember exactly what was discussed. We are experiencing >> problems with TdT staining. We get nothing on the core biopsies and only >> edge artifact staining on most clots. The FFPE thymus tissue looks GREAT. We >> are using Cell Marque's predilute antibody on the Ventana BenchMark XT. >> Any and all comments will be appreciated. >> Chris Jacobs, HT(ASCP), QIHC >> Jason Wheaton, HTL (ASCP) >> NorDx Labs >> Scarborough, ME >> >> CONFIDENTIALITY NOTICE: This email message, including any attachments, is >> for the use of the intended recipient(s) only and may contain information >> that is privileged, confidential, and prohibited from unauthorized >> disclosure under applicable law. If you are not the intended recipient of >> this message, any dissemination, distribution, or copying of this message is >> strictly prohibited. If you received this message in error, please notify >> the sender by reply email and destroy all copies of the original message and >> attachments. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From rfisher <@t> gbmc.org Fri Sep 12 15:38:59 2008 From: rfisher <@t> gbmc.org (RENEE FISHER) Date: Fri Sep 12 15:39:18 2008 Subject: [Histonet] please unsubscribe Message-ID: <48CA9B24.09C2.00CA.0@gbmc.org> please unsubscribe thank you _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. From tifei <@t> foxmail.com Fri Sep 12 21:31:29 2008 From: tifei <@t> foxmail.com (tf) Date: Fri Sep 12 21:31:58 2008 Subject: [Histonet] Improving the BrdU staining References: <20080911191659.BEB59005@m4500-03.uchicago.edu> Message-ID: <200809131031242715656@foxmail.com> RGVhciBBbGw6DQoNCkkgYW0gd3JpdGluZyB0byBkaXNjdXNzIHRoZSBmdW5jdGlvbiBvZiBlYWNo IHN0ZXAgaW4gQnJkVSBzdGFpbmluZy4NCkFzIHlvdSBtaWdodCBrbm93LCBDaXRyYXRlIGJ1ZmZl ciByZXRyaWV2YWwgYXQgOTUgZGVncmVlIGZvciAzMCBtaW4sIGFuZCAyTiBIQ2wgZm9yIGFub3Ro ZXIgMzAgbWluIGF0IFJUIGFyZSB0d28gY3JpdGljYWwgc3RlcHMgZm9yIEJyZFUgc3RhaW5pbmcu DQoNCigxKSBGb3IgY2l0cmF0ZSBidWZmZXIgcmV0cmlldmFsOiBJcyB0aGlzIHJlYWxseSBhbnRp Z2VuIHJldHJpZXZhbD8gT3Igd2UgYXJlIGp1c3QgdXNpbmcgY2l0cmF0ZSBidWZmZXIgdG8gcG9y aXplIHRoZSBjZWxsIG1lbWJyYW5lPyBGb3IgYW50aWdlbiByZXRyaWV2YWwgeW91IHNob3VsZCBz bG93bHkgY29vbCBkb3duIHRoZSBzbGlkZXMgaW4gY2l0cmF0ZSBidWZmZXIgZm9sbG93aW5nIGhl YXRpbmcgLSBidXQgSSBqdXN0IHRvb2sgdGhvc2Ugc2xpZGVzIG91dCBhbmQgcHV0IHRoZW0gaW4g 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cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0DQo= From talulahgosh <@t> gmail.com Fri Sep 12 23:27:05 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Sep 12 23:27:10 2008 Subject: [Histonet] please unsubscribe In-Reply-To: <48CA9B24.09C2.00CA.0@gbmc.org> References: <48CA9B24.09C2.00CA.0@gbmc.org> Message-ID: Are you new to the intertubes? Apparently, you are. Since that's the case, here's the address to unsubscribe THAT APPEARS AT THE END OF ALL EMAILS SENT TO HISTONET**. http://lists.utsouthwestern.edu/mailman/listinfo/histonet Emily **this includes the three unsubscribe messages you sent to histonet. -- the velocity of time turns her voice into sugar water http://www.youtube.com/watch?v=jNA6zzoObxg From carl.hobbs <@t> kcl.ac.uk Sat Sep 13 11:51:16 2008 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Sep 13 11:52:38 2008 Subject: [Histonet] Re: paraformaldehyde grade Message-ID: <11D9615B89C10747B1C985966A63D7CA2857ECB4F0@KCL-MAIL04.kclad.ds.kcl.ac.uk> Hi. I am interested in David's post: I too wished to change to Prills for weighing out PFA because the usual crystaline PFA is such a fine powder that it tends to "float" around, when weighing. ( conforming to Health and Safety recommendations by weighing the latter out in a fume hood.... The powder just went all over the place ;-) Hence "Prills".... i thought that they would be the answer! However, as Prills do not dissolve completely, as David indicated ( crystals dissolve completely), I judged that the additional filtration of the Prill-derived Formalin fixing fluid is another "complication" that is more likely to cause problems (spillage, inappropriate disposal of filters...for eg) So, I would be very grateful for any thoughts/recommendations on this. NB: Not practicable to use commercial vials as we do a lot of perfusions. Best wishes. Carl From Laura.Roan <@t> pbrc.edu Sat Sep 13 12:24:30 2008 From: Laura.Roan <@t> pbrc.edu (Laura Roan) Date: Sat Sep 13 12:24:37 2008 Subject: [Histonet] Leica Bond Message-ID: <6749EC8B7FC98943A6C22C4D16BF79461E64B6@pbrcas30.pbrc.edu> Hi Angela, My colleague, Courtney, and I have had a Bond Max on demo for a couple of months now and generally, we like it. We have also had demos from Dako and Lab Vision, but we are leaning more towards the Bond. This demo process has revealed that all the instruments have their strong points and weak points, and getting one instrument will have good and bad tradeoffs over another one, so you just have to pick what will most closely suit your lab needs with the fewest negative traits. We are in a research lab, so we have a wider range of needs from it than most clinical labs do, and the Bond has met most of these needs. We have tested thick tissues (30-60um) on it, and the staining is pretty good. The bigger challenge with the thick tissue, to our surprise, has been getting the tissue to adhere to the slide, not to get them to stain. Probably 75% of them have stayed, but we are still working with some other protocols for adherence, and I think we can get this number to increase. We have been really impressed with the service and support from Leica as well. They brought in two specialists on two different occasions to help us get things set up, one of them had a background mostly in research, which was a tremendous help. At one point, we were concerned about some differences in staining intensities between racks and within a day or so, a service rep was here to test the instrument. The bond seems to be well-made, and it has been fairly easy to use. The software could use some improving, especially for the research end, but it isn't bad enough that it would sway our opinions. We liked the software on the LabVision a little better, but this is mostly because it is a little more customizable, since we have many different protocols. I really like the feature of the Bond that all three of the racks work independently of one another, so you can start a run on one rack (as long as the times are the same in the protocols within a rack), and then later add another rack. This is not an option on the Dako or the LabVision. They have a "Stat Slide" feature which allows you to add slides during a run, but it will stop the first run, stain the new slides, and then finish the first run after. The Bond is also a little more automatic in that it does antigen retrieval on the instrument, not separately in a different apparatus. The bond can also heat the slides, which is not an option on the other two. Now for the things we don't like about it. First of all, it is NOT a completely open system, even if you buy the "research instrument with dongle." For the clinical instrument, you must buy their detection kits, even if you don't use them. For the research instrument, you are not locked into buying the detection kit, BUT you have to buy the research kit, which is simply a barcode to put on one of the reagent racks. AND, the research kit will expire after you use 40 mL out of any vile registered to the kit, in which case you have to buy another one. On top of that, in order to get the research option, you have to purchase the dongle (which only unlocks a few software features to allow the use of the research kit) and it costs 20 or 30 thousand dollars in addition to the cost of the instrument. The whole addition of the dongle/research kit also complicates the software a little bit as well. However, we have been bargaining with them since we are buying lots of other equipment as well, and I think we are going to reach a better agreement. There are some things that are not as customizable on the Bond as on the LabVision, such as the antigen retrieval. On the LabVision, you can retrieve as long as and as hot as and with whatever you want, whereas on the Bond, it is preset for both time and temperature and you have to use the Bond ER solutions. (Not always desirable for some of the tissues we will be using). Since we can't even trick the software into changing this, we would basically have to antigen retrieve elsewhere (ie pressure cooker offline) to customize our retrieval. Also, you are unable to incubate antibodies on tissues for more than 60 min at a time on the Bond, but you can trick it and add more of the same step, which will reapply the antibody for each step. We do like the mechanism of how the reagents are applied because it is very gentle, and seems to work for thick sections. Each slide is placed under a reusable, washable cover tile and the reagents are dispensed onto the end of the cover tile and then the reagent gently runs down the sections with the aid of a little vacuum port at the end of the slide. The covertile also prevents drying as well. The other two simply squirt the reagent onto the bare slide from a distance, which I don't like as much. Now that I've written a short book, I'll stop here. I think that is the majority of what we think about the stainers. I hope this helps and if you have any questions, please feel free to email me. Laura Laura E. Roan Cell Biology and Bioimaging Core Pennington Biomedical Research Center 6400 Perkins Road Baton Rouge, LA 70808 Email: RoanLE@pbrc.edu Phone: (225) 763-2653 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of angela smith Sent: Thu 8/28/2008 6:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond Is anyone using the Leica Bond Max IHC stainer? I will be evaluating it for several weeks and would like some feed back on those that are using it or have purchased it. Thank you, Angela _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Sat Sep 13 14:55:54 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Sep 13 14:55:59 2008 Subject: [Histonet] Stainers Message-ID: <582736990809131255q7b673f5egb91aaf2dfb539f6e@mail.gmail.com> Hi Joyce, We have an Autostainer (Dako) for the majority of our IHC. Often, however, I use a product from Thermo. The Sequenza IHC system is a bit bloated. It has a timer and a place to put the reagents ... kinda silly honestly. But, the Sequenza staining racks with cover plates (rest of the system aside) are nothing short of AWESOME! The racks hold 10 slides vertically which have cover plates on them. You put the reagents into a reservoir on top and they displace the previous reagent which drips out the bottom. The racks can be left in the fridge overnight if you like. The capillary gap prevents the reagents from sloshing around and giving you dried out sections like horizontal humidity chambers. BTW you can re-use the coverplates, just wash them really well and don't scratch or warp them. Separately I'll attach a pdf of the product because it is only in the older Thermo catalog, not the new one. Please note I do NOT work for Thermo or anything. I only reccommend really cool stuff that I've used and works well. "A (cool product) is a (cool product). That which (comes from one company) by any other name would still (be as cool)" -- Shakespeare -ish :-) Amos Message: 3 Date: Fri, 12 Sep 2008 12:25:07 -0500 From: "Rush, Joyce" Subject: [Histonet] Stainers To: Message-ID: <2337362E8548BE4C85EE5DD5D526B0850122C93A@sjw3smail2.SJMCMN.ORG> Content-Type: text/plain; charset="us-ascii" We are a low volume lab and currently do a limited menu of special and IHC stains on a stainer. We do our H&E's by hand. The stains for our IHC stainer are getting harder and harder to get. We are considering sending our IHC's out and getting a stainer for H&E and special stains. I'd appreciate any ideas from smaller volume labs. We do about 15,000 surgicals per year. I do apologize for asking about this as I know it's been covered over the years. Thanks so much! From contact <@t> excaliburpathology.com Sat Sep 13 16:21:08 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Sat Sep 13 16:21:13 2008 Subject: [Histonet] Stainers Message-ID: <451756.10919.qm@web1104.biz.mail.sk1.yahoo.com> I love my Sequenza. It is all I use. (Have 2 old Ventanas gathering dust) I only purchased the racks and?reuse the coverplates. I do not work for Thermo either. Paula Pierce Excalibur Pathology, Inc. 631 N Broadway Ave. Moore, OK 73160 405-759-3953 www.excaliburpathology.com ----- Original Message ---- From: Amos Brooks To: Joyce.Rush@sjmcmn.org; "histonet@lists.utsouthwestern.edu" Sent: Saturday, September 13, 2008 2:55:54 PM Subject: [Histonet] Stainers Hi Joyce, ? We have an Autostainer (Dako) for the majority of our IHC. Often, however,? I use a product from Thermo. The Sequenza IHC system is a bit bloated. It has a timer and a place to put the reagents ... kinda silly honestly. But, the Sequenza staining racks with cover plates (rest of the system aside) are nothing short of AWESOME! The racks hold 10 slides vertically which have cover plates on them. You put the reagents into a reservoir on top and they displace the previous reagent which drips out the bottom. The racks can be left in the fridge overnight if you like. The capillary gap prevents the reagents from sloshing around and giving you dried out sections like horizontal humidity chambers. BTW you can re-use the coverplates, just wash them really well and don't scratch or warp them. ? Separately I'll attach a pdf of the product because it is only in the older Thermo catalog, not the new one. Please note I do NOT work for Thermo or anything. I only reccommend really cool stuff that I've used and works well. "A (cool product) is a (cool product). That which (comes from one company) by any other name would still (be as cool)" -- Shakespeare -ish :-) Amos Message: 3 Date: Fri, 12 Sep 2008 12:25:07 -0500 From: "Rush, Joyce" Subject: [Histonet] Stainers To: Message-ID: ? ? ? <2337362E8548BE4C85EE5DD5D526B0850122C93A@sjw3smail2.SJMCMN.ORG> Content-Type: text/plain;? ? ? charset="us-ascii" We are a low volume lab and currently do a limited menu of special and IHC stains on a stainer.? We do our H&E's by hand.? The stains for our IHC stainer are getting harder and harder to get.? We are considering sending our IHC's out and getting a stainer for H&E and special stains. I'd appreciate any ideas from smaller volume labs.? We do about 15,000 surgicals per year.? I do apologize for asking about this as I know it's been covered over the years. Thanks so much! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From haozhang1 <@t> gmail.com Sat Sep 13 22:11:55 2008 From: haozhang1 <@t> gmail.com (hao zhang) Date: Sat Sep 13 22:11:59 2008 Subject: [Histonet] antigen retrieval for 5 year pap smear slides Message-ID: <65992c0c0809132011oc935550q1952710d7d6b6a23@mail.gmail.com> Hi, everyone I want to do IHC from a 5 year old pap smear slides for pan-keratin stain. I tried many methods of antigen retrieval protocols (10 mM citric buffer, ph 6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at 95C for 40 minutes. I also used Triton x 100 (0.2%) pretreatment 10 minutes and then SDS (0.5% or 0.25%) treatment for 10 minutes. But all method did not work. Any replies will be very appreciated. -- Hao ZHANG, Ph.D Scientist From rjbuesa <@t> yahoo.com Sun Sep 14 07:45:14 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Sep 14 07:45:18 2008 Subject: [Histonet] antigen retrieval for 5 year pap smear slides In-Reply-To: <65992c0c0809132011oc935550q1952710d7d6b6a23@mail.gmail.com> Message-ID: <767109.81120.qm@web65716.mail.ac4.yahoo.com> Usually PAP smears are NOT fixed with NBF so they should NOT be treated with HIER before doing IHC. If you have some spare smear do IHC directly (after destaining). Ren? J. --- On Sat, 9/13/08, hao zhang wrote: From: hao zhang Subject: [Histonet] antigen retrieval for 5 year pap smear slides To: histonet@lists.utsouthwestern.edu Date: Saturday, September 13, 2008, 11:11 PM Hi, everyone I want to do IHC from a 5 year old pap smear slides for pan-keratin stain. I tried many methods of antigen retrieval protocols (10 mM citric buffer, ph 6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at 95C for 40 minutes. I also used Triton x 100 (0.2%) pretreatment 10 minutes and then SDS (0.5% or 0.25%) treatment for 10 minutes. But all method did not work. Any replies will be very appreciated. -- Hao ZHANG, Ph.D Scientist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Sun Sep 14 12:00:27 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Sun Sep 14 12:02:38 2008 Subject: [Histonet] RE: Normal solution In-Reply-To: <221007.43462.qm@web38606.mail.mud.yahoo.com> References: <221007.43462.qm@web38606.mail.mud.yahoo.com> Message-ID: Since there is only one mole of ammonium ion in each mole of ammonium chloride, 1 M = 1 N. Thus 0.1 mole of ammonium chloride in 0.1 liter of water is a 1 N solution. Thus 5.35 g ammonium chloride in 100 ml of water is a 1 N solution. ________________________________ From: Ramadan El Sokary [ramadan_elsokary2003@yahoo.com] Sent: Sunday, September 14, 2008 5:29 AM To: Smith, Allen; histonet@pathology.swmed.edu Subject: Normal solution Help! Can someone tell me exactly how to make a 1 N solution of Ammonium chloride? From AnthonyH <@t> chw.edu.au Sun Sep 14 19:26:28 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Sep 14 19:26:41 2008 Subject: [Histonet] antigen retrieval for 5 year pap smear slides In-Reply-To: <65992c0c0809132011oc935550q1952710d7d6b6a23@mail.gmail.com> Message-ID: Do not bother to retrieve. Usually PAP smears are not fixed in formalin but are alcohol fixed. Alcohol is an excellent fixative for intermediate filaments as well as keratins. I would just de-coverslip the slides, rinse in buffer, block endogenous enzyme, add your diluted antibody (you will probably find that you may need to dilute your antibody further than for FFPE sections (maybe up to 1-100 times more)) and finally demonstrate the bound antibody. For titreing prepare some buccal smears, fix in the same fixative used for the PAP smears (95% ethanol or whatever commercial Cyto spray fix that is used), and work up the titreing as usual. No antigen retrieval is required unless formalin cross-links have been formed. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hao zhang Sent: Sunday, 14 September 2008 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antigen retrieval for 5 year pap smear slides Hi, everyone I want to do IHC from a 5 year old pap smear slides for pan-keratin stain. I tried many methods of antigen retrieval protocols (10 mM citric buffer, ph 6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at 95C for 40 minutes. I also used Triton x 100 (0.2%) pretreatment 10 minutes and then SDS (0.5% or 0.25%) treatment for 10 minutes. But all method did not work. Any replies will be very appreciated. -- Hao ZHANG, Ph.D Scientist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Sep 14 21:49:22 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Sep 14 21:49:33 2008 Subject: [Histonet] antigen retrieval for 5 year pap smear slides In-Reply-To: <65992c0c0809141922o375e713vd11157be3852d3a5@mail.gmail.com> Message-ID: Hao, It might be possible that not all of the mountant has been removed. Though I am surprised that the staining is not better. It just might be one of those instances where the keratin epitopes are damaged during the 5 year storage. Possibly the phosphotungstic acid, often included in the PAP stains, might also be effecting the epitopes. Again try these variables on buccal or sputum smears and compare the results. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: hao zhang [mailto:haozhang1@gmail.com] Sent: Monday, 15 September 2008 12:22 PM To: Tony Henwood Subject: Re: [Histonet] antigen retrieval for 5 year pap smear slides Hi, Tony, Ren? and all, I agree that pap smear is fixed by alcohol instead of formalin. Problem for me now is that when I do IHC for pan-keratin stain I only find small part cell stained (around 10%) even when I dont do antigen retrival by HIER. I know it is around 5 year old slides but I dont know why I can not get higher positive percentage because I used Hela cell as a control I got around 90% positive percentage. In addition, I find a lot of pink color material remained in the slide. I used 3% acid alcohol for destaining treatment and destaining effect get improved but finally I still get less positive percentage for pan-keratin. any suggest will be very appreciated. On Sun, Sep 14, 2008 at 5:26 PM, Tony Henwood wrote: Do not bother to retrieve. Usually PAP smears are not fixed in formalin but are alcohol fixed. Alcohol is an excellent fixative for intermediate filaments as well as keratins. I would just de-coverslip the slides, rinse in buffer, block endogenous enzyme, add your diluted antibody (you will probably find that you may need to dilute your antibody further than for FFPE sections (maybe up to 1-100 times more)) and finally demonstrate the bound antibody. For titreing prepare some buccal smears, fix in the same fixative used for the PAP smears (95% ethanol or whatever commercial Cyto spray fix that is used), and work up the titreing as usual. No antigen retrieval is required unless formalin cross-links have been formed. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hao zhang Sent: Sunday, 14 September 2008 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antigen retrieval for 5 year pap smear slides Hi, everyone I want to do IHC from a 5 year old pap smear slides for pan-keratin stain. I tried many methods of antigen retrieval protocols (10 mM citric buffer, ph 6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at 95C for 40 minutes. I also used Triton x 100 (0.2%) pretreatment 10 minutes and then SDS (0.5% or 0.25%) treatment for 10 minutes. But all method did not work. Any replies will be very appreciated. -- Hao ZHANG, Ph.D Scientist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** -- Hao ZHANG, Ph.D Scientist Diamics, Inc ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From nfournier <@t> sasktel.net Mon Sep 15 02:38:48 2008 From: nfournier <@t> sasktel.net (Neil Fournier) Date: Mon Sep 15 02:39:00 2008 Subject: [Histonet] help! buffer calculation Message-ID: <000801c91706$18379bf0$da9b2fcf@NEIL> To make a 1-L 0.2 M phosphate buffer stock my recipe calls for 21.87 g Na2HPO4 (anhydrous dibasic) and 6.35 g NaH2PO4.H2O (monohydrate monobasic) dissolved in 1-L of water. (fairly standard procedure). I am sure my undergraduate chemistry professor is turning over in his grave, but I am embarrass to say that I am having difficulty figuring out how the amount of anhydrous dibasic and monohydrate monobasic was determined. Could someone please show me the calculations. I have played around with Henderson-Hasselbalch equation to no end. Much obliged Neil From kgreen <@t> hsh.org Mon Sep 15 08:05:58 2008 From: kgreen <@t> hsh.org (Green, Kathy) Date: Mon Sep 15 08:06:50 2008 Subject: [Histonet] question about Her2 neu Message-ID: First, is there a time frame involved for fixation for formalin issue when running Her2neu? If it is FDA approved is it necessary to monitor it (time frames). We are having a problem on our trials that the ours are running higher (ours would be a 1+ vs. 0) than the company we previously sent them to. Could it be due to the fact that they don't have an FDA approved HER 2 neu and maybe not from the same company (we use Ventana antibodies/equipment) that we use? Thanks for any help or pointers in this area. Please respond to my personal email any responses. Kathy Green, HT Manager Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 kgreen@hsh.org (717) 763-2930 Blackberry: (717) 370-1726 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From rjbuesa <@t> yahoo.com Mon Sep 15 08:19:32 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Sep 15 08:19:36 2008 Subject: [Histonet] question about Her2 neu In-Reply-To: Message-ID: <403231.48037.qm@web65703.mail.ac4.yahoo.com> The ASCO/CAP recommendation is 6 to 8 hours fixation in NBF and even this fixation period does not assure an effective crosslinking. The fact that they are biopsies is irrelevant because the NBF fixation time is the same for specimens from 160?m to 4 mm thick (the crosslinking time is independent of tissue thickness). You cannot compare your results to others unless you know exactly how your methodology compares. M wrote: From: Green, Kathy Subject: [Histonet] question about Her2 neu To: histonet@lists.utsouthwestern.edu Date: Monday, September 15, 2008, 9:05 AM First, is there a time frame involved for fixation for formalin issue when running Her2neu? If it is FDA approved is it necessary to monitor it (time frames). We are having a problem on our trials that the ours are running higher (ours would be a 1+ vs. 0) than the company we previously sent them to. Could it be due to the fact that they don't have an FDA approved HER 2 neu and maybe not from the same company (we use Ventana antibodies/equipment) that we use? Thanks for any help or pointers in this area. Please respond to my personal email any responses. Kathy Green, HT Manager Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 kgreen@hsh.org (717) 763-2930 Blackberry: (717) 370-1726 From gu.lang <@t> gmx.at Mon Sep 15 09:09:09 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Sep 15 09:09:35 2008 Subject: AW: [Histonet] question about Her2 neu In-Reply-To: Message-ID: <07000CF975E64CD286AB239B330449F7@dielangs.at> We had the problem, that our protocol with the dako-antibody on the benchmark xt gave also too high scores. You have to validate your protocol against a "gold-standard". That would be for example the hercep-test. We switched to the ventana-antibody, with the effect, that the 3+ nearly disappeard in our statistic. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Green, Kathy Gesendet: Montag, 15. September 2008 15:06 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] question about Her2 neu First, is there a time frame involved for fixation for formalin issue when running Her2neu? If it is FDA approved is it necessary to monitor it (time frames). We are having a problem on our trials that the ours are running higher (ours would be a 1+ vs. 0) than the company we previously sent them to. Could it be due to the fact that they don't have an FDA approved HER 2 neu and maybe not from the same company (we use Ventana antibodies/equipment) that we use? Thanks for any help or pointers in this area. Please respond to my personal email any responses. Kathy Green, HT Manager Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 kgreen@hsh.org (717) 763-2930 Blackberry: (717) 370-1726 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gedinite <@t> gmail.com Mon Sep 15 11:24:36 2008 From: gedinite <@t> gmail.com (Richard Nelson) Date: Mon Sep 15 11:24:41 2008 Subject: [Histonet] 2030 microtomes Message-ID: I've got two 2030 microtomes if anyone is interested. Rick gedinite@gmail.com From Rcartun <@t> harthosp.org Mon Sep 15 11:33:09 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Sep 15 11:33:25 2008 Subject: [Histonet] antigen retrieval for 5 year pap smear slides In-Reply-To: <65992c0c0809132011oc935550q1952710d7d6b6a23@mail.gmail.com> References: <65992c0c0809132011oc935550q1952710d7d6b6a23@mail.gmail.com> Message-ID: <48CE56050200007700005630@gwmail6.harthosp.org> Are your PAP smear slides fixed in alcohol? If so, you probably don't need antigen retrieval. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "hao zhang" 09/13/08 11:11 PM >>> Hi, everyone I want to do IHC from a 5 year old pap smear slides for pan-keratin stain. I tried many methods of antigen retrieval protocols (10 mM citric buffer, ph 6; 1 mM EDTA, Ph8; 10 mM Tris buffer, Ph 10; ) and heat at 95C for 40 minutes. I also used Triton x 100 (0.2%) pretreatment 10 minutes and then SDS (0.5% or 0.25%) treatment for 10 minutes. But all method did not work. Any replies will be very appreciated. -- Hao ZHANG, Ph.D Scientist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Vacca <@t> HCAhealthcare.com Mon Sep 15 12:05:58 2008 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Mon Sep 15 12:06:29 2008 Subject: [Histonet] question about Her2 neu In-Reply-To: <403231.48037.qm@web65703.mail.ac4.yahoo.com> References: <403231.48037.qm@web65703.mail.ac4.yahoo.com> Message-ID: <41E16A15CE78374EA45B57E0F94339B8045B82F5@ORLEV01.hca.corpad.net> Does this all pertain to if you do Her2nu in-house or for all Breast whether you send them out in the event a breast profile has been requested? We do not do them in-house. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, September 15, 2008 9:20 AM To: histonet@lists.utsouthwestern.edu; Green, Kathy Subject: Re: [Histonet] question about Her2 neu The ASCO/CAP recommendation is 6 to 8 hours fixation in NBF and even this fixation period does not assure an effective crosslinking. The fact that they are biopsies is irrelevant because the NBF fixation time is the same for specimens from 160?m to 4 mm thick (the crosslinking time is independent of tissue thickness). You cannot compare your results to others unless you know exactly how your methodology compares. M wrote: From: Green, Kathy Subject: [Histonet] question about Her2 neu To: histonet@lists.utsouthwestern.edu Date: Monday, September 15, 2008, 9:05 AM First, is there a time frame involved for fixation for formalin issue when running Her2neu? If it is FDA approved is it necessary to monitor it (time frames). We are having a problem on our trials that the ours are running higher (ours would be a 1+ vs. 0) than the company we previously sent them to. Could it be due to the fact that they don't have an FDA approved HER 2 neu and maybe not from the same company (we use Ventana antibodies/equipment) that we use? Thanks for any help or pointers in this area. Please respond to my personal email any responses. Kathy Green, HT Manager Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 kgreen@hsh.org (717) 763-2930 Blackberry: (717) 370-1726 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Mon Sep 15 13:32:26 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Sep 15 13:32:57 2008 Subject: [Histonet] Ventana Symphony query In-Reply-To: <40026EDDE64CDA47AB382C52619ACD3C0A09E147@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: Anyone have any comments on the use of the Venana Symphony for H+E? Jackie O' From NMargaryan <@t> childrensmemorial.org Mon Sep 15 15:14:18 2008 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Sep 15 15:15:42 2008 Subject: [Histonet] Endogenous Peroxide Treatment -IHC In-Reply-To: References: Message-ID: Hi histonetters, I fully agree with Isaac. We all know that the liver always gives a background even if 4 steps of blocking were used (H2O2, Avidin, Biotin, Protein Block). Why in TMAs always we found a liver as a control sample, which gives us false result??????????????? Thanks, Naira >>>>>>>>>>>>>>>>>>>>>> Message: 2 Date: Sat, 15 Mar 2008 11:29:08 -0700 From: "Isaac Milos" Subject: RE: [Histonet] Endogenous Peroxide Treatment - Immunohistochemistry To: "Marilyn Johnson" , Message-ID: <7F2A2AE306CE254DB7279E86A51A740657BE92@CMROCEX01.cellmarque.local> Content-Type: text/plain; charset="us-ascii" Hi Marilyn, If you are running your IHC stains with an HRP detection kit with a biotinylated secondary antibody, endogenous biotin within the tissue can cause background staining to occur. Often times, you'll see endogenous biotin in a tissue that is particularly bloody, such as liver, kidney, or brain. You can use an avidin/biotin block before secondary antibody is applied to account for this. You can find this block through many different companies - mine (Cell Marque) offers a good version. The part number is CMX222 and it can be ordered at 1-800-665-7284. Happy staining! Isaac From cfranci <@t> rigel.com Mon Sep 15 16:11:00 2008 From: cfranci <@t> rigel.com (Christian Franci) Date: Mon Sep 15 16:11:08 2008 Subject: [Histonet] necrosis Message-ID: Hello Histonetters! I was just wondering what is the best way to look for necrosis on FFPE tissue sections. Is H&E the best way or are there other ways to visualize it that are more striking? I need to take lots of photos for presentations and I'm searching for a stain/ IHC that is easy to see. Thanks a bunch, Chris From cbass <@t> wfubmc.edu Mon Sep 15 16:49:05 2008 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Mon Sep 15 16:49:14 2008 Subject: [Histonet] EGFP IHC in floating brain sections Message-ID: Hello, I?m hoping someone could help me troubleshoot my staining protocol. I am staining to EGFP in microglia (produced by a virus I injected in the brain). Here?s my general protocol: Deactive endogenous peroxidases (0.1M PB + 20% methanol + titon-x100 (80 ul in 40 ml total) + 0.3% h2O2) for 15 min. Wash 3X15min in 0.1MPB+0.3%titon Incubate in 0.1MPB+0.3%triton+1%normal donkey serum for 1 hr Incubate with primary (goat anti-GFP from Rockland) overnight at 4 degrees (1:1000) Wash 3x15 min 0.1M PB + 0.3% triton Incubate in with secondary (Jackson Donkey anti-goat, biotin labeled) overnight at 4 degrees (1:500) Wash 3x15 min in 0.1M PB Use ABC kit and vector SG for substrate (can?t remember how long I let it develop, but it was within a couple of minutes). So, the bottom line is that I did a test section and I thought it had a lot of background, so I incubated the other sections for less time. However, when I took a closer look, the sections with little background had no microglial signal, while the one with high background had a very clear and noticeable signal in the place it should be. So, the question is how to cut back on the noise. Would increasing the secondary concentration to 1:1000 help? I?m following a protocol someone else came up with, but it seems strange that the secondary is at a higher concentration than the primary. Thanks! Caroline From AnthonyH <@t> chw.edu.au Mon Sep 15 18:12:55 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Sep 15 18:13:07 2008 Subject: [Histonet] Endogenous Peroxide Treatment -IHC In-Reply-To: Message-ID: >>"Why in TMAs always we found a liver as a control sample, which gives us false result???????????????">> Probably because it does. If using the ABC demonstration method, I would always include a small sample of liver in my negative control to gauge the technique's ability to block endogenous biotin. And would you believe that the amount of biotin stained would vary from batch to batch (why??- maybe slide drying temp/time OR efficiency of antigen retrieval OR age of biotinylates antibody OR age of blocking solutions OR ....) For this reason we now use polymer technology. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Tuesday, 16 September 2008 6:14 AM To: histonet@lists.utsouthwestern.edu Cc: Marilyn Johnson Subject: [Histonet] Endogenous Peroxide Treatment -IHC Hi histonetters, I fully agree with Isaac. We all know that the liver always gives a background even if 4 steps of blocking were used (H2O2, Avidin, Biotin, Protein Block). Why in TMAs always we found a liver as a control sample, which gives us false result??????????????? Thanks, Naira >>>>>>>>>>>>>>>>>>>>>> Message: 2 Date: Sat, 15 Mar 2008 11:29:08 -0700 From: "Isaac Milos" Subject: RE: [Histonet] Endogenous Peroxide Treatment - Immunohistochemistry To: "Marilyn Johnson" , Message-ID: <7F2A2AE306CE254DB7279E86A51A740657BE92@CMROCEX01.cellmarque.local> Content-Type: text/plain; charset="us-ascii" Hi Marilyn, If you are running your IHC stains with an HRP detection kit with a biotinylated secondary antibody, endogenous biotin within the tissue can cause background staining to occur. Often times, you'll see endogenous biotin in a tissue that is particularly bloody, such as liver, kidney, or brain. You can use an avidin/biotin block before secondary antibody is applied to account for this. You can find this block through many different companies - mine (Cell Marque) offers a good version. The part number is CMX222 and it can be ordered at 1-800-665-7284. Happy staining! Isaac _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Mon Sep 15 18:14:55 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Sep 15 18:15:00 2008 Subject: [Histonet] necrosis In-Reply-To: Message-ID: A good H&E is easiest. I have used poly T mRNA in situ hybridisation to demonstrate areas of necrosis quite well (absence of signal) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christian Franci Sent: Tuesday, 16 September 2008 7:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] necrosis Hello Histonetters! I was just wondering what is the best way to look for necrosis on FFPE tissue sections. Is H&E the best way or are there other ways to visualize it that are more striking? I need to take lots of photos for presentations and I'm searching for a stain/ IHC that is easy to see. Thanks a bunch, Chris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From nyilmaz <@t> mersin.edu.tr Tue Sep 16 01:17:12 2008 From: nyilmaz <@t> mersin.edu.tr (Nejat Yilmaz) Date: Tue Sep 16 01:17:51 2008 Subject: [Histonet] BrdU incorporating and staining Message-ID: <000b01c917c3$db9ab2e0$6b01a8c0@nejat1> Dear Netters, We are setting up a cell therapy model in neonate rats by injecting primary cultured astrocytes intracerebrally. My questions are: 1. Is it possible to incorporate BrdU in all cells by adding it 10 uM concentration into culture media from beginning of the culture? 2. If so, can we demonstrate BrdU in confluenced cell nuclei by using IHC or IF on petri culture dishes? (Fixed with %4 PF for 20 min.) 3. Should we use detergents or any other agents for facilitating penetration of the antibodies in to the nuclei, any special protocol suggestion? 4. If we accomplish this difficulties, can we demonstrate these labelled donor cells in host animal brains a few weeks later by using IHC or IF? And the last thing I have to point out is: unfortunately we don't have any possibility to obtain knock-in animal or vector virus treated cells. We would be really appreciated your helpful comments. Necat Yilmaz From tifei <@t> foxmail.com Tue Sep 16 03:29:42 2008 From: tifei <@t> foxmail.com (tf) Date: Tue Sep 16 03:30:18 2008 Subject: [Histonet] BrdU incorporating and staining References: <000b01c917c3$db9ab2e0$6b01a8c0@nejat1> Message-ID: <200809161629374952134@foxmail.com> aGksDQoxLiBubyBpZGVhLg0KMi4geWVzLiBpZiB0aGV5IHByb2xpZmVyYXRlLg0KMy4ganVzdCB0 cmVhdCB3aXRoIFBCU1QgKFRyaXMtYnVmZmVyZWQgUEJTKSBiZWZvcmUgeW91ciBhZGRpbmcgb2Yg YW50aWJvZHkuDQo0LiBwb3NzaWJseS4uanVzdCBub3RpY2Ugc29tZSBCcmRVIHBvc2l0aXZlIGNl bGxzIGNvdWxkIGJlIG1pY3JvZ2xpYS4uYmUgY2FyZWZ1bCBvZiB0aGUgbGVha2FnZS4uYW5kIGlm IHlvdSBkb250IGhhdmUgYSBHRlAtcmF0LCBoYXZlIHlvdSBjb25zaWRlcmVkIG9mIHVzaW5nIGFz dHJvY3l0ZXMgZnJvbSBtaWNlIGZvciBpbmplY3Rpb24gaW50byB0aGUgcmF0IGJyYWluPw0KDQoN CjIwMDgtMDktMTYgDQoNCg0KDQp0ZiANCg0KDQoNCreivP7Iy6O6IE5lamF0IFlpbG1heiANCrei y83Ksbzko7ogMjAwOC0wOS0xNiAgMTQ6MjQ6MTEgDQrK1bz+yMujuiBoaXN0b25ldEBsaXN0cy51 dHNvdXRod2VzdGVybi5lZHUgDQqzrcvNo7ogDQrW98zio7ogW0hpc3RvbmV0XSBCcmRVIGluY29y cG9yYXRpbmcgYW5kIHN0YWluaW5nIA0KIA0KRGVhciBOZXR0ZXJzLA0KV2UgYXJlIHNldHRpbmcg dXAgYSBjZWxsIHRoZXJhcHkgbW9kZWwgaW4gbmVvbmF0ZSByYXRzIGJ5IGluamVjdGluZyAgcHJp bWFyeSANCmN1bHR1cmVkIGFzdHJvY3l0ZXMgaW50cmFjZXJlYnJhbGx5Lg0KTXkgcXVlc3Rpb25z IGFyZToNCjEuIElzIGl0IHBvc3NpYmxlIHRvIGluY29ycG9yYXRlIEJyZFUgaW4gYWxsIGNlbGxz IGJ5IGFkZGluZyBpdCAxMCB1TSANCmNvbmNlbnRyYXRpb24gaW50byBjdWx0dXJlIG1lZGlhIGZy b20gYmVnaW5uaW5nIG9mIHRoZSBjdWx0dXJlPw0KMi4gSWYgc28sIGNhbiB3ZSBkZW1vbnN0cmF0 ZSBCcmRVIGluIGNvbmZsdWVuY2VkIGNlbGwgbnVjbGVpIGJ5IHVzaW5nIElIQyBvciANCklGIG9u IHBldHJpIGN1bHR1cmUgZGlzaGVzPyAoRml4ZWQgd2l0aCAlNCBQRiBmb3IgMjAgbWluLikNCjMu IFNob3VsZCB3ZSB1c2UgZGV0ZXJnZW50cyBvciBhbnkgb3RoZXIgYWdlbnRzIGZvciBmYWNpbGl0 YXRpbmcgcGVuZXRyYXRpb24gDQpvZiB0aGUgYW50aWJvZGllcyBpbiB0byB0aGUgbnVjbGVpLCBh bnkgc3BlY2lhbCBwcm90b2NvbCBzdWdnZXN0aW9uPw0KNC4gSWYgd2UgYWNjb21wbGlzaCB0aGlz IGRpZmZpY3VsdGllcywgY2FuIHdlIGRlbW9uc3RyYXRlIHRoZXNlIGxhYmVsbGVkIA0KZG9ub3Ig Y2VsbHMgaW4gaG9zdCBhbmltYWwgYnJhaW5zIGEgZmV3IHdlZWtzIGxhdGVyIGJ5IHVzaW5nIElI QyBvciBJRj8NCkFuZCB0aGUgbGFzdCB0aGluZyBJIGhhdmUgdG8gcG9pbnQgb3V0IGlzOiB1bmZv cnR1bmF0ZWx5IHdlIGRvbid0IGhhdmUgYW55IA0KcG9zc2liaWxpdHkgdG8gb2J0YWluIGtub2Nr LWluIGFuaW1hbCBvciB2ZWN0b3IgdmlydXMgdHJlYXRlZCBjZWxscy4NCldlIHdvdWxkIGJlIHJl YWxseSBhcHByZWNpYXRlZCB5b3VyIGhlbHBmdWwgY29tbWVudHMuDQpOZWNhdCBZaWxtYXogDQpf X19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fXw0KSGlzdG9uZXQg bWFpbGluZyBsaXN0DQpIaXN0b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUNCg== From ian.montgomery <@t> bio.gla.ac.uk Tue Sep 16 07:47:11 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Tue Sep 16 07:47:24 2008 Subject: [Histonet] Slides. Message-ID: <8A5DCCBB9FD24D1DA993567B01C04E35@IBLS.GLA.AC.UK> Does anyone know of a UK supplier for large slides? I'm talking twice, three times wider than normal. Look at your wrist, that size. Plus of course, coverglasses. We've been using old glass E.M. plates stripped off emulsion but this supply is rapidly dwindling. Isn't it great working with anatomists? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From PMonfils <@t> Lifespan.org Tue Sep 16 08:55:56 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Sep 16 08:56:09 2008 Subject: [Histonet] Slides. In-Reply-To: <8A5DCCBB9FD24D1DA993567B01C04E35@IBLS.GLA.AC.UK> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C5B@LSRIEXCH1.lsmaster.lifespan.org> Hope you find a supplier in UK. I get my large slides and coverglasses from http://www.brainresearchlab.com/ From dlschneider <@t> gmail.com Tue Sep 16 09:43:20 2008 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Tue Sep 16 09:43:25 2008 Subject: [Histonet] Storage of unstained slides? (was Re: Saving unstained slides Message-ID: <1085e7000809160743y5fb605edwb913b73647163457@mail.gmail.com> Thank you everyone, that was very helpful information. How do you store your unstained slides? Specifically, how do you store them during the initial period between cutting and sign-out, and, if they are kept after sign-out, how are they stored, if differently? We have a not so insignificant volume, and I'm concerned that my histotechnologists will kill me, so practical advice as far as how to do this in a work-flow friendly, organized, efficient fashion is appreciated. Obviously preserving/protecting the exposed tissue on the slide is of key importance. Also, our lab is not unusual in that space is scarce. Thanks! Daniel Schneider From gagnone <@t> KGH.KARI.NET Tue Sep 16 09:56:18 2008 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Tue Sep 16 09:56:32 2008 Subject: [Histonet] Storage of Unstained Slides Message-ID: Hi Daniel, Between cutting and sign-out, we keep unstained slides in our 60-degree oven overnight, or longer if there are section adhesion problems. Then we store them in baskets or on trays at room temperature. A day's unstained slides do not take up too much bench space if stored this way. Once the case is signed out, our unstained slides are filed with all our stained slides. We will generally take slides in for filing when the tray gets full, usually about once a week. Over time, we should probably stop storing these unstained slides, once we can convince pathologists to use fresh-cut sections. That change is coming, but I don't think we're there yet. They do take up a lot of room when dealing with a not so insignificant volume, you're right! Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From SAllen <@t> exchange.hsc.mb.ca Tue Sep 16 10:01:16 2008 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Tue Sep 16 10:01:25 2008 Subject: [Histonet] NSE histochemical stain Message-ID: Hi, I am looking for some help with NSE histology staining on frozen muscle tissue. I would really like a method, with all the little tricks (if any), that is working well for someone. I have been trying the method from Dubowitz (Muscle Biopsy, A Practical Approach & the Armed Forces Institute of Pathology. But am not getting a strong reaction. Having never seen a positive slide I nor the Neuropathologist know for sure what we are to be seeing. Does anyone have a picture of a slide they would be willing to share with us. Thanks in advance for any help you can give me. Sharon Allen Neuropathology Lab Health Sciences Centre Wpg, MB Canada sallen@hsc.mb.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From tojo <@t> novonordisk.com Tue Sep 16 10:11:13 2008 From: tojo <@t> novonordisk.com (TOJO (Torben Seested Johansen)) Date: Tue Sep 16 10:11:22 2008 Subject: [Histonet] (no subject) Message-ID: <070F7D8AAB80E049B8D6583739F5C9AE096E98ECB3@exdkmbx005.corp.novocorp.net> Hi, I do not seem to be able to achieve acceptble morphology of perfused rat liver. I seems as if some of the cytosol of the hepatocytes are "washed away" as seen in toluidine blue stained cryo-sections (see image18.jpg). I am to use the tissue cryo-sections in immunohistochemistry and transmission electron microscopy. I my attempts to get an acceptable/good morphology I have tried fixation buffers consisting of 2, 4, 6 or 8 % paraformaldehyde (w/wo 0.1% glutaraldehyde). My fixing procedure is as follows (all at room temperature); a rat (~250g) is anasthestized and a perfusion needle is inserted into the left ventricle. The atrium is cut and PBS is flushed thru the rat at 20ml/min for 5min. The rat is thereafter fixated at 10ml/min for 10 min with fixation buffer (I have so far tried 2, 4, 6 or 8 % paraformaldehyde (with and with out 0.1% glutaraldehyde) in 0.1M cacodylatebuffer (pH 7.4) all with same unacceptable result (see image18.jpg). The liver is cut into smaller pieces (~2*2*2mm) post fixed for 1h in the respective fixative, transferred into 2.3M sucrose for minimum 1h (preferably over night) and frozen in liquid nitrogen. Any idea why teh cytosol of my hepatocytes seems to be "gone" (hydropic degeneration?) ? I have searched the net and it seems as if theres a 1000's of different ways of performing rat liver perfusion fixation. Some use cold buffers and others also perfuse with sucrose solution (either in combination with the fixative or with sucrose alone post-fixation) what can I do to try and optimise my perfusion ? ______________________________________ Torben Seested Johansen Post Doc Exploratory ADME, Biopharmaceuticals Novo Nordisk A/S Novo Nordisk Park E9.S.22 DK-2760 M?l?v Denmark +45 44 43 14 84 (direct) +45 44 66 39 39 (fax) tojo@novonordisk.com www.novonordisk.com This e-mail (including any attachments) is intended for the addressee(s) stated above only and may contain confidential information protected by law. You are hereby notified that any unauthorized reading, disclosure, copying or distribution of this e-mail or use of information contained herein is strictly prohibited and may violate rights to proprietary information. If you are not an intended recipient, please return this e-mail to the sender and delete it immediately hereafter. Thank you. From Dorothy.L.Webb <@t> HealthPartners.Com Tue Sep 16 10:16:51 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Sep 16 10:16:59 2008 Subject: [Histonet] Amyloid Message-ID: <0E394B648E5284478A6CCB78E5AFDA270563597D@hpes1.HealthPartners.int> We have been cutting our tissue for amyloid staining @ 8 microns. One of my pathologists heard that 10 microns is now standard and to use a negative and weakly positive control. Does anyone have any new information in this area? Thanks ahead of time! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From STRAUSSJ <@t> upstate.edu Tue Sep 16 10:26:50 2008 From: STRAUSSJ <@t> upstate.edu (Judy Strauss) Date: Tue Sep 16 10:27:31 2008 Subject: [Histonet] FFPE mouse tissue problem Message-ID: <48CF97FA020000A400022252@gwmta2.upstate.edu> Good Morning, For the first time I find myself working with decalcified mouse tissue and all is not well. Bone is fine but the muscle tissue in the block is "crispy" and shreds when sectioned. I do not know if the tissue is over/under fixed or over/under processed. I would like to know why mouse tissue behaves so differently from rat. I would appreciate a protocol (along with helpful hints) for embedding decalcified mouse hind limbs in paraffin. Thanks in advance, Judith Strauss SUNY Upstate Medical University Department of Orthopedic Surgery IHP room 3118 505 Irving Avenue Syracuse, NY 13120 phone: (315) 464-9960 fax: (315) 464-6638 From rjbuesa <@t> yahoo.com Tue Sep 16 11:33:53 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 16 11:34:01 2008 Subject: [Histonet] FFPE mouse tissue problem In-Reply-To: <48CF97FA020000A400022252@gwmta2.upstate.edu> Message-ID: <711293.55071.qm@web65706.mail.ac4.yahoo.com> The difference resides in the fat contents. Decalcification tends to increase the difficulties. If you have enough time to process, try to decalcify using EDTA instead of an acid decalcification. Your dehydration times should also be shortened and use 2-propanol instead of ethanol, and if you want to really overcome the difficulties, substitute xylene with mixtures of 2-propanol:mineral oil (5:1 and 2:1) at 50?C followed by pure mineral oil at 50?C before your usual paraffin baths.? Ren? J. --- On Tue, 9/16/08, Judy Strauss wrote: From: Judy Strauss Subject: [Histonet] FFPE mouse tissue problem To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 16, 2008, 11:26 AM Good Morning, For the first time I find myself working with decalcified mouse tissue and all is not well. Bone is fine but the muscle tissue in the block is "crispy" and shreds when sectioned. I do not know if the tissue is over/under fixed or over/under processed. I would like to know why mouse tissue behaves so differently from rat. I would appreciate a protocol (along with helpful hints) for embedding decalcified mouse hind limbs in paraffin. Thanks in advance, Judith Strauss SUNY Upstate Medical University Department of Orthopedic Surgery IHP room 3118 505 Irving Avenue Syracuse, NY 13120 phone: (315) 464-9960 fax: (315) 464-6638 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Sep 16 11:36:06 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 16 11:36:13 2008 Subject: [Histonet] Amyloid In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA270563597D@hpes1.HealthPartners.int> Message-ID: <608057.46402.qm@web65703.mail.ac4.yahoo.com> No, but you should ask your pathologist where that "standard" comes from. S/he should know since s/he brought the issue up. Ren? J. --- On Tue, 9/16/08, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] Amyloid To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 16, 2008, 11:16 AM We have been cutting our tissue for amyloid staining @ 8 microns. One of my pathologists heard that 10 microns is now standard and to use a negative and weakly positive control. Does anyone have any new information in this area? Thanks ahead of time! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Sep 16 11:36:54 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 16 11:36:58 2008 Subject: [Histonet] Amyloid In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA270563597D@hpes1.HealthPartners.int> Message-ID: <861313.44069.qm@web65714.mail.ac4.yahoo.com> No, but you should ask your pathologist where that "standard" comes from. S/he should know since s/he brought the issue up. Ren? J. --- On Tue, 9/16/08, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] Amyloid To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 16, 2008, 11:16 AM We have been cutting our tissue for amyloid staining @ 8 microns. One of my pathologists heard that 10 microns is now standard and to use a negative and weakly positive control. Does anyone have any new information in this area? Thanks ahead of time! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tchistology <@t> earthlink.net Tue Sep 16 12:45:39 2008 From: tchistology <@t> earthlink.net (Randall Carpenter) Date: Tue Sep 16 12:45:42 2008 Subject: [Histonet] Looking for H&E schedule for Varistain 24-4 Message-ID: <16648622.1221587139951.JavaMail.root@elwamui-royal.atl.sa.earthlink.net> Greetings Histonet, It's been a while since I was here and now I'm back. I'm now Twin Cities Histology and it's a busy business right now. I just purchased a used Varistain 24-4 and was wondering if anyone had a good schedule for the Harris Hematoxylin (regressive). I've been doing the AFP method by hand for the last year or so, but now I'm up against it and had to buy the machine. Any help would be appreciated. Thanks. Randy Carpenter Twin Cities Histology From mcauliff <@t> umdnj.edu Tue Sep 16 13:17:48 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Sep 16 13:17:25 2008 Subject: [Histonet] (no subject) In-Reply-To: <070F7D8AAB80E049B8D6583739F5C9AE096E98ECB3@exdkmbx005.corp.novocorp.net> References: <070F7D8AAB80E049B8D6583739F5C9AE096E98ECB3@exdkmbx005.corp.novocorp.net> Message-ID: <48CFF84C.2060502@umdnj.edu> Dear Tojo: Your problem could be due to 3 things. 1. Waiting too long to fix the tissue. 2. Freezing too slowly. 3. Interpreting the lack of staining of hepatocyte glycogen as an artifact. 1. You are waiting 5 minutes after death to fix the tissue. There is absolutely NO reason to spend 5 min pumping 100 ml of PBS through the circ. system. You will never wash out all of the blood and there is no need to. AND when you finally get to fixative, the flow of fixative is too low. Here is the solution to your problem. Use a 16 g needle in the L. vent. Pump 10-15 ml of room temperature PBS into the rat in 15-20 seconds. Pump 300 ml of room temperatue fix in 5 minutes. The fix is 4% paraformaldehyde for light microscopy. For transmission EM the fix is 2 or 3 or 4% paraformaldehyde with 1% glutaraldehyde. Buffer can be either phosphate or cacodylate. The advantage of cac. buffer is that you can add some calcium salts to stabilize membranes for EM (2milleMole) without percipitation.Yes, you can add Ca salts to phos. buffer but it will precipitate instantly (look up the solubility of CaPhosphate, or should I say the insolubility). Of course, cacodylate has arsenic in it so don't lick your fingers. Remove liver and cut into appropriate sized pieces. For light microscopy fix as long as possible, formalin reacts with tissue very slowly. Glutaraldehyde fixed much faster, an hour or two is plenty. Cryoprotect with sucrose. 2. Tissue must be frozen VERY QUICKLY with 2-methylbutane (isopentane) cooled with dry ice or liquid nitrogen otherwise you will have large holes due to ice crystal formation. 3. The stain you are using, Tol.Blue will not stain glycogen so you may have empty-looking areas because of this. Use Periodic acid Schiff for glycogen but NOT after glutaraldehyde fixation. Geoff TOJO (Torben Seested Johansen) wrote: > Hi, > I do not seem to be able to achieve acceptble morphology of perfused rat liver. I seems as if some of the cytosol of the hepatocytes are "washed away" as seen in toluidine blue stained cryo-sections (see image18.jpg). I am to use the tissue cryo-sections in immunohistochemistry and transmission electron microscopy. > > I my attempts to get an acceptable/good morphology I have tried fixation buffers consisting of 2, 4, 6 or 8 % paraformaldehyde (w/wo 0.1% glutaraldehyde). > > My fixing procedure is as follows (all at room temperature); > > a rat (~250g) is anasthestized and a perfusion needle is inserted into the left ventricle. The atrium is cut and PBS is flushed thru the rat at 20ml/min for 5min. The rat is thereafter fixated at 10ml/min for 10 min with fixation buffer (I have so far tried 2, 4, 6 or 8 % paraformaldehyde (with and with out 0.1% glutaraldehyde) in 0.1M cacodylatebuffer (pH 7.4) all with same unacceptable result (see image18.jpg). The liver is cut into smaller pieces (~2*2*2mm) post fixed for 1h in the respective fixative, transferred into 2.3M sucrose for minimum 1h (preferably over night) and frozen in liquid nitrogen. > > Any idea why teh cytosol of my hepatocytes seems to be "gone" (hydropic degeneration?) ? > > I have searched the net and it seems as if theres a 1000's of different ways of performing rat liver perfusion fixation. Some use cold buffers and others also perfuse with sucrose solution (either in combination with the fixative or with sucrose alone post-fixation) > > what can I do to try and optimise my perfusion ? > > ______________________________________ > > Torben Seested Johansen > Post Doc > Exploratory ADME, Biopharmaceuticals > > Novo Nordisk A/S > Novo Nordisk Park > E9.S.22 > DK-2760 M?l?v > Denmark > +45 44 43 14 84 (direct) > +45 44 66 39 39 (fax) > tojo@novonordisk.com > www.novonordisk.com > > This e-mail (including any attachments) is intended for the addressee(s) stated above only and may contain confidential information protected by law. You are hereby notified that any unauthorized reading, disclosure, copying or distribution of this e-mail or use of information contained herein is strictly prohibited and may violate rights to proprietary information. If you are not an intended recipient, please return this e-mail to the sender and delete it immediately hereafter. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From azdudley <@t> hotmail.com Tue Sep 16 13:52:42 2008 From: azdudley <@t> hotmail.com (anita dudley) Date: Tue Sep 16 13:52:48 2008 Subject: [Histonet] (no subject) Message-ID: job in fairhope alabama, beautiful area along the bay. dematologist office looking for someone to do the mohs surgery. contact anita, providence hosp. mobile alabama, 251-633-1422. _________________________________________________________________ See how Windows connects the people, information, and fun that are part of your life. http://clk.atdmt.com/MRT/go/msnnkwxp1020093175mrt/direct/01/ From Maxim_71 <@t> mail.ru Tue Sep 16 14:05:58 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Tue Sep 16 14:08:34 2008 Subject: [Histonet] Amyloid Message-ID: <596251904.20080916230558@mail.ru> Dorothy: We does Highman's method (Bancroft&Stevens, 1977 p.164) Both 8 and 10 microns give good results. Maxim Peshkov Russia, Taganrog. mailto:Maxim_71@mail.ru From mburton1 <@t> bu.edu Tue Sep 16 14:31:30 2008 From: mburton1 <@t> bu.edu (Mark A Burton) Date: Tue Sep 16 14:31:56 2008 Subject: [Histonet] Amyloid In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA270563597D@hpes1.HealthPartners.int> References: <0E394B648E5284478A6CCB78E5AFDA270563597D@hpes1.HealthPartners.int> Message-ID: <000c01c91832$d24f65e0$76ee31a0$@edu> Dorothy, We've done several comparisons like this for amyloid staining. In our experience, 8um works fine. We did see stronger staining (birefringence) in some thicker sections with Congo Red but I don't think there would be a significant improvement in staining (sensitivity?) from 8um to 10um. I wouldn't cut sections any thinner than 8um but you can still get amyloid staining at 4um. It would be relatively easy to take your controls and cut them different thicknesses just to prove the point. Good luck! Mark A Burton HTL ASCP Lab Manager & Sr. Histotechnologist Molecular Aging & Development Lab Boston University Medical Campus -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, September 16, 2008 11:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amyloid We have been cutting our tissue for amyloid staining @ 8 microns. One of my pathologists heard that 10 microns is now standard and to use a negative and weakly positive control. Does anyone have any new information in this area? Thanks ahead of time! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Tue Sep 16 14:32:08 2008 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Sep 16 14:32:15 2008 Subject: [Histonet] NSH class Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701EB6B95@giamail2.Gia.com> Hi! I was wondering if anyone was going to NSH and attending the class for "Are you Ready for the HT board of Registry Exam"? by speaker: Robert L Lott, BS, HTL (ASCP), Trinity Medial Center/LabFirst, Inc., Birmingham, AL ? I am not able to attend NSH this year, and I'm really interested in the material that Robert will be presenting about. I am already HT certified, but I want to take the HTL soon and thought this class would be a good start for study material. I emailed Pebbles at NSH for the speakers email address to contact him personally, but I never heard back from her. If anyone is planning on going to this class, could you send me a copy of any handouts? Thanks, Amber McKenzie, B.S., HT (ASCP) 1405 N. State St., Suite 400 Jackson, MS 39202 (ph) 601-863-0388 (fax) 601-326-3532 From kynemitz <@t> travmax.com Tue Sep 16 15:24:29 2008 From: kynemitz <@t> travmax.com (Kyla Nemitz) Date: Tue Sep 16 15:24:51 2008 Subject: [Histonet] Permanent histo job in Austin, TX Message-ID: <9416D9FA37C1C04FA83D69684E95D2E71E4DDD95@exbk2.maxhealth.com> Hello all - I am looking for an ASCP Histo tech for a permanent position in Austin, TX. The position is day shift, M-F and pays $55-60K/year. The facility is also helping with relocation. If you or anyone you know is currently looking to relocation to Texas, please contact me. Also - I specialize in placing histo techs on travel and permanent positions Nationwide. If you have any questions or are looking for a position, please feel free to call or email. Thank you in advance, your help is appreciated! Kyla Nemitz TravelMax Medical Professionals - Maxim Healthcare * 888.800.1855 or 813.371.5175 7 800.294.1248 www.TravelMaxAllied.com From Herrick.James <@t> mayo.edu Tue Sep 16 17:11:46 2008 From: Herrick.James <@t> mayo.edu (Herrick, James L.) Date: Tue Sep 16 17:12:10 2008 Subject: [Histonet] Question on staining Message-ID: Hi all, I am trying to stain for osteoid and bone in pig tibia (need to quantify osteoid and bone volumes). Does anyone have experience with these types of stains in 40-60 ?m thick, MMA embedded, sections? I have tried a Masson's trichrome and Toluidine blue, but have been unable to get well defined stain differentiation with this thick of sections. I would really appreciate any help I can get. Thanks again. Jim From PVerden <@t> UROPARTNERS.COM Wed Sep 17 10:29:04 2008 From: PVerden <@t> UROPARTNERS.COM (Paul Verden) Date: Wed Sep 17 10:29:12 2008 Subject: [Histonet] stain Message-ID: I am interested in finding the Alternate Bielschowsky Stain Protocol. There are problems getting the silver to clear. From Barry.R.Rittman <@t> uth.tmc.edu Wed Sep 17 10:38:58 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Sep 17 10:39:06 2008 Subject: [Histonet] Question on staining In-Reply-To: References: Message-ID: There is a question on the validity of measuring osteoid on thick sections. The reason is that it is rare in bone to have sections where edges are absolutely vertical throughout the section. You therefore have several planes superimposed and several edges in these planes resulting in a penumbra effect. The greater the angle to the vertical edge of the bone such as edges of trabeculae or Haversian canals, the greater the errors in measuring. The most accurate measurements are those utilizing sections that are 5 microns or below. Is there some particular reason that you must use sections this thick? Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. Sent: Tuesday, September 16, 2008 5:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question on staining Hi all, I am trying to stain for osteoid and bone in pig tibia (need to quantify osteoid and bone volumes). Does anyone have experience with these types of stains in 40-60 ?m thick, MMA embedded, sections? I have tried a Masson's trichrome and Toluidine blue, but have been unable to get well defined stain differentiation with this thick of sections. I would really appreciate any help I can get. Thanks again. Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From azdudley <@t> hotmail.com Wed Sep 17 10:50:47 2008 From: azdudley <@t> hotmail.com (anita dudley) Date: Wed Sep 17 10:50:51 2008 Subject: [Histonet] job opening Message-ID: sorry I failed to put anything in the subject line on the previous note. anita job is in fairhope ala. mohs lab in need of a histologist. 251-633-1422 _________________________________________________________________ Get more out of the Web. Learn 10 hidden secrets of Windows Live. http://windowslive.com/connect/post/jamiethomson.spaces.live.com-Blog-cns!550F681DAD532637!5295.entry?ocid=TXT_TAGLM_WL_domore_092008 From brod033 <@t> gmail.com Wed Sep 17 10:58:01 2008 From: brod033 <@t> gmail.com (Ben Spirto) Date: Wed Sep 17 10:58:38 2008 Subject: [Histonet] stain In-Reply-To: References: Message-ID: <287d127c0809170858g53d73752v6cb4152870407ff1@mail.gmail.com> *Bielschowski Stain* *20% Silver Nitrate Solution:* 1. Silver Nitrate=20g 2. dWater=100ml *Ammonia Water:* 1. dWater=100ml 2. Ammonium Hydroxide=8 drops *Developer:* 1. dWater=100ml 2. Formalin=20ml 3. Citric Acid=0.5g 4. Concentrated Nitric Acid=2 drops *Carcinogenic* *5%Sodium Thiosulfate (Hypo)* 1. Sodium Thiosulfate=5g 2.dWater=100ml Ammonium Silver Solution 1. 20% silver nitrate=100ml 2. Ammonium Hydroxide=10ml Protocol: On the stir plate to the 20% silver nitrate solution add 10-15 ml of ammonium hydroxide, until brown precipitate forms, adding one drop at the time. Keep adding more of ammonium hydroxide until solution is clear (while vigorously stirring). Add couple drops of 20 % of silver nitrate until solution is gold. Slides are placed in the ammonium silver solution in the dark for 15-20 min. After that slides are placed in ammonia water. To the ammonium silver solution add the developing solution (developer) in the ratio of 8 drops of developer per 100ml of solution while stirring. Slides are developed 3-5 min On Wed, Sep 17, 2008 at 10:29 AM, Paul Verden wrote: > I am interested in finding the Alternate Bielschowsky Stain Protocol. > There are problems getting the silver to clear. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ratliffjack <@t> hotmail.com Wed Sep 17 11:08:21 2008 From: ratliffjack <@t> hotmail.com (Jack Ratliff ) Date: Wed Sep 17 11:08:28 2008 Subject: [Histonet] Question on staining Message-ID: Jim, I would like to respond to your question regarding the pig tibia. After reading Barry's reply, I too question why you would not want to cut thin (5 micron) sections. It then occurred to me that maybe you are not able to do so because this would require the use of a sledge or polycut microtome. Given the size of your specimen, I agree that you you will have better and more consistent results with your histomorphometry endpoints if you cut thinner sections using a Reicher-Jung Polycut or a Leica SM2500. Upon completion, you can then deplastify the sections (if you use MMA) and yield excellent staining results by employing a Von Kossa reaction with a MacNeals tetrachrome counterstain and/or a standard Goldners trichrome stain. If you need further assistance or more information, please feel free to contact me Jack Ratliff -----Original Message----- From: Rittman Barry R Sent: Wednesday, September 17, 2008 11:39 AM To: Herrick.James@mayo.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Question on staining There is a question on the validity of measuring osteoid on thick sections. The reason is that it is rare in bone to have sections where edges are absolutely vertical throughout the section. You therefore have several planes superimposed and several edges in these planes resulting in a penumbra effect. The greater the angle to the vertical edge of the bone such as edges of trabeculae or Haversian canals, the greater the errors in measuring. The most accurate measurements are those utilizing sections that are 5 microns or below. Is there some particular reason that you must use sections this thick? Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. Sent: Tuesday, September 16, 2008 5:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question on staining Hi all, I am trying to stain for osteoid and bone in pig tibia (need to quantify osteoid and bone volumes). Does anyone have experience with these types of stains in 40-60 ?m thick, MMA embedded, sections? I have tried a Masson's trichrome and Toluidine blue, but have been unable to get well defined stain differentiation with this thick of sections. I would really appreciate any help I can get. Thanks again. Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jm.lapointe <@t> accellab.com Wed Sep 17 12:28:00 2008 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Wed Sep 17 12:29:21 2008 Subject: [Histonet] Re: (no subject) (Geoff McAuliffe) References: <200809171709.m8HH9b2I008087@gateway5.lastspam.com> Message-ID: Tojo, Geoff provides very good pointers, but I think his option 3. is the correct one in your case, i.e. what looks like 'holes' are areas of glycogen and/or lipid that are unstained with the technique you are using. If your rats are not fasted prior to euthanasia, the cytoplasm of the hepatocytes will be filled with glycogen. Trying a PAS would likely confirm this, by staining the clear areas positive. Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, dACVP AccelLAB Inc ? ------------------------------ Date: Tue, 16 Sep 2008 14:17:48 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] (no subject) To: "TOJO (Torben Seested Johansen)" Dear Tojo: Your problem could be due to 3 things. 1. Waiting too long to fix the tissue. 2. Freezing too slowly. 3. Interpreting the lack of staining of hepatocyte glycogen as an artifact. 1. You are waiting 5 minutes after death to fix the tissue. There is absolutely NO reason to spend 5 min pumping 100 ml of PBS through the circ. system. You will never wash out all of the blood and there is no need to. AND when you finally get to fixative, the flow of fixative is too low. Here is the solution to your problem. Use a 16 g needle in the L. vent. Pump 10-15 ml of room temperature PBS into the rat in 15-20 seconds. Pump 300 ml of room temperatue fix in 5 minutes. The fix is 4% paraformaldehyde for light microscopy. For transmission EM the fix is 2 or 3 or 4% paraformaldehyde with 1% glutaraldehyde. Buffer can be either phosphate or cacodylate. The advantage of cac. buffer is that you can add some calcium salts to stabilize membranes for EM (2milleMole) without percipitation.Yes, you can add Ca salts to phos. buffer but it will precipitate instantly (look up the solubility of CaPhosphate, or should I say the insolubility). Of course, cacodylate has arsenic in it so don't lick your fingers. Remove liver and cut into appropriate sized pieces. For light microscopy fix as long as possible, formalin reacts with tissue very slowly. Glutaraldehyde fixed much faster, an hour or two is plenty. Cryoprotect with sucrose. 2. Tissue must be frozen VERY QUICKLY with 2-methylbutane (isopentane) cooled with dry ice or liquid nitrogen otherwise you will have large holes due to ice crystal formation. 3. The stain you are using, Tol.Blue will not stain glycogen so you may have empty-looking areas because of this. Use Periodic acid Schiff for glycogen but NOT after glutaraldehyde fixation. Geoff TOJO (Torben Seested Johansen) wrote: > Hi, > I do not seem to be able to achieve acceptble morphology of perfused rat liver. I seems as if some of the cytosol of the hepatocytes are "washed away" as seen in toluidine blue stained cryo-sections (see image18.jpg). I am to use the tissue cryo-sections in immunohistochemistry and transmission electron microscopy. > > I my attempts to get an acceptable/good morphology I have tried fixation buffers consisting of 2, 4, 6 or 8 % paraformaldehyde (w/wo 0.1% glutaraldehyde). > > My fixing procedure is as follows (all at room temperature); > > a rat (~250g) is anasthestized and a perfusion needle is inserted into the left ventricle. The atrium is cut and PBS is flushed thru the rat at 20ml/min for 5min. The rat is thereafter fixated at 10ml/min for 10 min with fixation buffer (I have so far tried 2, 4, 6 or 8 % paraformaldehyde (with and with out 0.1% glutaraldehyde) in 0.1M cacodylatebuffer (pH 7.4) all with same unacceptable result (see image18.jpg). The liver is cut into smaller pieces (~2*2*2mm) post fixed for 1h in the respective fixative, transferred into 2.3M sucrose for minimum 1h (preferably over night) and frozen in liquid nitrogen. > > Any idea why teh cytosol of my hepatocytes seems to be "gone" (hydropic degeneration?) ? > > I have searched the net and it seems as if theres a 1000's of different ways of performing rat liver perfusion fixation. Some use cold buffers and others also perfuse with sucrose solution (either in combination with the fixative or with sucrose alone post-fixation) > > what can I do to try and optimise my perfusion ? > > ______________________________________ > > Torben Seested Johansen > Post Doc > Exploratory ADME, Biopharmaceuticals From mroark <@t> sfmc.net Wed Sep 17 12:39:31 2008 From: mroark <@t> sfmc.net (Matt Roark) Date: Wed Sep 17 12:39:47 2008 Subject: [Histonet] Helicobacter Message-ID: <000a01c918ec$5a178890$06570181@sfmc.net> What is everyone using for a Helicobacter control (non-ihc)? We are currently buying our Helico control slides from Master Tech and using the Diff Quick method to stain them. Our pathologist is not very happy with the controls so we need to find a different source. Any help would be appreciated! Thanks!!! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 From cbass <@t> wfubmc.edu Wed Sep 17 13:12:19 2008 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Wed Sep 17 13:12:48 2008 Subject: [Histonet] EGFP IHC in floating brain sections In-Reply-To: Message-ID: Hello, Sorry if this is a double post but I didn't see my first one in the digest. I?m hoping someone could help me troubleshoot my staining protocol. I am staining to EGFP in microglia (produced by a virus I injected in the brain). Here?s my general protocol: Deactive endogenous peroxidases (0.1M PB + 20% methanol + titon-x100 (80 ul in 40 ml total) + 0.3% h2O2) for 15 min. Wash 3X15min in 0.1MPB+0.3%titon Incubate in 0.1MPB+0.3%triton+1%normal donkey serum for 1 hr Incubate with primary (goat anti-GFP from Rockland) overnight at 4 degrees (1:1000) Wash 3x15 min 0.1M PB + 0.3% triton Incubate in with secondary (Jackson Donkey anti-goat, biotin labeled) overnight at 4 degrees (1:500) Wash 3x15 min in 0.1M PB Use ABC kit and vector SG for substrate (can?t remember how long I let it develop, but it was within a couple of minutes). So, the bottom line is that I did a test section and I thought it had a lot of background, so I incubated the other sections for less time. However, when I took a closer look, the sections with little background had no microglial signal, while the one with high background had a very clear and noticeable signal in the place it should be. So, the question is how to cut back on the noise. Would increasing the secondary concentration to 1:1000 help? I?m following a protocol someone else came up with, but it seems strange that the secondary is at a higher concentration than the primary. Thanks! Caroline From Rcartun <@t> harthosp.org Wed Sep 17 13:24:50 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Sep 17 13:25:01 2008 Subject: [Histonet] Helicobacter In-Reply-To: <000a01c918ec$5a178890$06570181@sfmc.net> References: <000a01c918ec$5a178890$06570181@sfmc.net> Message-ID: <48D1133202000077000057F3@gwmail6.harthosp.org> Why not use a positive case from your laboratory? Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Matt Roark" 09/17/08 1:39 PM >>> What is everyone using for a Helicobacter control (non-ihc)? We are currently buying our Helico control slides from Master Tech and using the Diff Quick method to stain them. Our pathologist is not very happy with the controls so we need to find a different source. Any help would be appreciated! Thanks!!! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Wed Sep 17 13:32:01 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Sep 17 13:32:06 2008 Subject: [Histonet] Helicobacter In-Reply-To: <000a01c918ec$5a178890$06570181@sfmc.net> References: <000a01c918ec$5a178890$06570181@sfmc.net> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82E0C@EMAIL.archildrens.org> We use positive cases from our GI biopsies. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Roark Sent: Wednesday, September 17, 2008 12:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter What is everyone using for a Helicobacter control (non-ihc)? We are currently buying our Helico control slides from Master Tech and using the Diff Quick method to stain them. Our pathologist is not very happy with the controls so we need to find a different source. Any help would be appreciated! Thanks!!! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From dmlaud <@t> gmail.com Wed Sep 17 13:33:29 2008 From: dmlaud <@t> gmail.com (Damien) Date: Wed Sep 17 13:33:39 2008 Subject: [Histonet] Re: Osteoid staning (Damien Laudier) Message-ID: <6f5a847e0809171133u761fbf62q66184784ae3e5019@mail.gmail.com> Hi Jim, Does your study require Osteoid measurements in the transverse plane (you did not mention this)? If so, obviously you will need to use ground sections (30-40 microns). If your samples have not been processed, I would recommend doing an osteochrome bulk stain (the Villanueva method) and take your measurements using fluorescence. (Osteoid will be a vivid orange). Even if you've already processed, there are still options for using thick sections. Assuming your cutting, polishing and mounting techniques are consistent ,you won't have any measurement problems. It is perfectly acceptable to use thick sections for osteoid measurement. -Damien Laudier On Wed, Sep 17, 2008 at 1:01 PM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Looking for H&E schedule for Varistain 24-4 (Randall Carpenter) > 2. Re: (no subject) (Geoff McAuliffe) > 3. (no subject) (anita dudley) > 4. Re: Amyloid (Maxim_71@mail.ru) > 5. RE: Amyloid (Mark A Burton) > 6. NSH class (Amber McKenzie) > 7. Permanent histo job in Austin, TX (Kyla Nemitz) > 8. Question on staining (Herrick, James L.) > 9. stain (Paul Verden) > 10. RE: Question on staining (Rittman, Barry R) > 11. job opening (anita dudley) > 12. Re: stain (Ben Spirto) > 13. RE: Question on staining (Jack Ratliff ) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 16 Sep 2008 10:45:39 -0700 (GMT-07:00) > From: Randall Carpenter > Subject: [Histonet] Looking for H&E schedule for Varistain 24-4 > To: Histonet > Message-ID: > < > 16648622.1221587139951.JavaMail.root@elwamui-royal.atl.sa.earthlink.net> > > Content-Type: text/plain; charset=UTF-8 > > Greetings Histonet, > > It's been a while since I was here and now I'm back. I'm now Twin Cities > Histology and it's a busy business right now. I just purchased a used > Varistain > 24-4 and was wondering if anyone had a good schedule for the Harris > Hematoxylin > (regressive). I've been doing the AFP method by hand for the last year or > so, > but now I'm up against it and had to buy the machine. Any help would be > appreciated. > Thanks. > > Randy Carpenter > Twin Cities Histology > > > > ------------------------------ > > Message: 2 > Date: Tue, 16 Sep 2008 14:17:48 -0400 > From: Geoff McAuliffe > Subject: Re: [Histonet] (no subject) > To: "TOJO (Torben Seested Johansen)" > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: <48CFF84C.2060502@umdnj.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Dear Tojo: > > Your problem could be due to 3 things. 1. Waiting too long to fix > the tissue. 2. Freezing too slowly. 3. Interpreting the lack of staining > of hepatocyte glycogen as an artifact. > > 1. You are waiting 5 minutes after death to fix the tissue. There is > absolutely NO reason to spend 5 min pumping 100 ml of PBS through the > circ. system. You will never wash out all of the blood and there is no > need to. AND when you finally get to fixative, the flow of fixative is > too low. Here is the solution to your problem. > > Use a 16 g needle in the L. vent. > Pump 10-15 ml of room temperature PBS into the rat in 15-20 seconds. > Pump 300 ml of room temperatue fix in 5 minutes. The fix is 4% > paraformaldehyde for light microscopy. For transmission EM the fix is 2 > or 3 or 4% paraformaldehyde with 1% glutaraldehyde. Buffer can be > either phosphate or cacodylate. The advantage of cac. buffer is that you > can add some calcium salts to stabilize membranes for EM (2milleMole) > without percipitation.Yes, you can add Ca salts to phos. buffer but it > will precipitate instantly (look up the solubility of CaPhosphate, or > should I say the insolubility). Of course, cacodylate has arsenic in it > so don't lick your fingers. > Remove liver and cut into appropriate sized pieces. For light microscopy > fix as long as possible, formalin reacts with tissue very slowly. > Glutaraldehyde fixed much faster, an hour or two is plenty. > Cryoprotect with sucrose. > 2. Tissue must be frozen VERY QUICKLY with 2-methylbutane (isopentane) > cooled with dry ice or liquid nitrogen otherwise you will have large > holes due to ice crystal formation. > 3. The stain you are using, Tol.Blue will not stain glycogen so you may > have empty-looking areas because of this. Use Periodic acid Schiff for > glycogen but NOT after glutaraldehyde fixation. > > Geoff > > > TOJO (Torben Seested Johansen) wrote: > > Hi, > > I do not seem to be able to achieve acceptble morphology of perfused rat > liver. I seems as if some of the cytosol of the hepatocytes are "washed > away" as seen in toluidine blue stained cryo-sections (see image18.jpg). I > am to use the tissue cryo-sections in immunohistochemistry and transmission > electron microscopy. > > > > I my attempts to get an acceptable/good morphology I have tried fixation > buffers consisting of 2, 4, 6 or 8 % paraformaldehyde (w/wo 0.1% > glutaraldehyde). > > > > My fixing procedure is as follows (all at room temperature); > > > > a rat (~250g) is anasthestized and a perfusion needle is inserted into > the left ventricle. The atrium is cut and PBS is flushed thru the rat at > 20ml/min for 5min. The rat is thereafter fixated at 10ml/min for 10 min with > fixation buffer (I have so far tried 2, 4, 6 or 8 % paraformaldehyde (with > and with out 0.1% glutaraldehyde) in 0.1M cacodylatebuffer (pH 7.4) all with > same unacceptable result (see image18.jpg). The liver is cut into smaller > pieces (~2*2*2mm) post fixed for 1h in the respective fixative, transferred > into 2.3M sucrose for minimum 1h (preferably over night) and frozen in > liquid nitrogen. > > > > Any idea why teh cytosol of my hepatocytes seems to be "gone" (hydropic > degeneration?) ? > > > > I have searched the net and it seems as if theres a 1000's of different > ways of performing rat liver perfusion fixation. Some use cold buffers and > others also perfuse with sucrose solution (either in combination with the > fixative or with sucrose alone post-fixation) > > > > what can I do to try and optimise my perfusion ? > > > > ______________________________________ > > > > Torben Seested Johansen > > Post Doc > > Exploratory ADME, Biopharmaceuticals > > > > Novo Nordisk A/S > > Novo Nordisk Park > > E9.S.22 > > DK-2760 M?l?v > > Denmark > > +45 44 43 14 84 (direct) > > +45 44 66 39 39 (fax) > > tojo@novonordisk.com > > www.novonordisk.com > > > > This e-mail (including any attachments) is intended for the addressee(s) > stated above only and may contain confidential information protected by law. > You are hereby notified that any unauthorized reading, disclosure, copying > or distribution of this e-mail or use of information contained herein is > strictly prohibited and may violate rights to proprietary information. If > you are not an intended recipient, please return this e-mail to the sender > and delete it immediately hereafter. Thank you. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 16 Sep 2008 13:52:42 -0500 > From: anita dudley > Subject: [Histonet] (no subject) > To: "histonet@pathology.swmed.edu" > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > job in fairhope alabama, beautiful area along the bay. dematologist office > looking for someone to do the mohs surgery. contact anita, providence hosp. > mobile alabama, 251-633-1422. > _________________________________________________________________ > See how Windows connects the people, information, and fun that are part of > your life. > http://clk.atdmt.com/MRT/go/msnnkwxp1020093175mrt/direct/01/ > > ------------------------------ > > Message: 4 > Date: Tue, 16 Sep 2008 23:05:58 +0400 > From: Maxim_71@mail.ru > Subject: Re: [Histonet] Amyloid > To: Dorothy.L.Webb@HealthPartners.Com > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <596251904.20080916230558@mail.ru> > Content-Type: text/plain; charset=us-ascii > > Dorothy: > We does Highman's method (Bancroft&Stevens, 1977 p.164) > Both 8 and 10 microns give good results. > Maxim Peshkov > Russia, > Taganrog. > mailto:Maxim_71@mail.ru > > > > > ------------------------------ > > Message: 5 > Date: Tue, 16 Sep 2008 15:31:30 -0400 > From: "Mark A Burton" > Subject: RE: [Histonet] Amyloid > To: "'Webb, Dorothy L'" , > > Message-ID: <000c01c91832$d24f65e0$76ee31a0$@edu> > Content-Type: text/plain; charset="US-ASCII" > > Dorothy, > > We've done several comparisons like this for amyloid staining. In our > experience, 8um works fine. We did see stronger staining (birefringence) in > some thicker sections with Congo Red but I don't think there would be a > significant improvement in staining (sensitivity?) from 8um to 10um. I > wouldn't cut sections any thinner than 8um but you can still get amyloid > staining at 4um. It would be relatively easy to take your controls and cut > them different thicknesses just to prove the point. Good luck! > > > Mark A Burton HTL ASCP > Lab Manager & Sr. Histotechnologist > Molecular Aging & Development Lab > Boston University Medical Campus > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Tuesday, September 16, 2008 11:17 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Amyloid > > We have been cutting our tissue for amyloid staining @ 8 microns. One > of my pathologists heard that 10 microns is now standard and to use a > negative and weakly positive control. Does anyone have any new > information in this area? Thanks ahead of time! > > Dorothy Webb, HT (ASCP) > Histology Technical Supervisor > Regions Hospital, Pathology Department > 640 Jackson Street, Saint Paul, MN 55101-2595 > Phone: 651-254-2962 > Fax: 651-254-2741 > Regions Hospital is part of the HealthPartners family of care > ________________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 6 > Date: Tue, 16 Sep 2008 14:32:08 -0500 > From: "Amber McKenzie" > Subject: [Histonet] NSH class > To: > Message-ID: > <03C921A1EAF7F541B16543F6EC6A4B3701EB6B95@giamail2.Gia.com> > Content-Type: text/plain; charset="us-ascii" > > Hi! I was wondering if anyone was going to NSH and attending the class > for "Are you Ready for the HT board of Registry Exam"? by speaker: > Robert L Lott, BS, HTL (ASCP), Trinity Medial Center/LabFirst, Inc., > Birmingham, AL ? I am not able to attend NSH this year, and I'm really > interested in the material that Robert will be presenting about. I am > already HT certified, but I want to take the HTL soon and thought this > class would be a good start for study material. I emailed Pebbles at NSH > for the speakers email address to contact him personally, but I never > heard back from her. If anyone is planning on going to this class, > could you send me a copy of any handouts? > > > > Thanks, > > Amber McKenzie, B.S., HT (ASCP) > > 1405 N. State St., Suite 400 > > Jackson, MS 39202 > > (ph) 601-863-0388 > > (fax) 601-326-3532 > > > > > > > > ------------------------------ > > Message: 7 > Date: Tue, 16 Sep 2008 16:24:29 -0400 > From: "Kyla Nemitz" > Subject: [Histonet] Permanent histo job in Austin, TX > To: > Message-ID: > <9416D9FA37C1C04FA83D69684E95D2E71E4DDD95@exbk2.maxhealth.com> > Content-Type: text/plain; charset="us-ascii" > > Hello all - > > > > I am looking for an ASCP Histo tech for a permanent position in Austin, > TX. The position is day shift, M-F and pays $55-60K/year. The facility > is also helping with relocation. > > > > If you or anyone you know is currently looking to relocation to Texas, > please contact me. > > > > Also - I specialize in placing histo techs on travel and permanent > positions Nationwide. If you have any questions or are looking for a > position, please feel free to call or email. > > > > Thank you in advance, your help is appreciated! > > > > Kyla Nemitz > > TravelMax Medical Professionals - Maxim Healthcare > > * 888.800.1855 or 813.371.5175 > > 7 800.294.1248 > > www.TravelMaxAllied.com < > http://www.travelmaxallied.com/> > > > > > > ------------------------------ > > Message: 8 > Date: Tue, 16 Sep 2008 17:11:46 -0500 > From: "Herrick, James L." > Subject: [Histonet] Question on staining > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Hi all, > > I am trying to stain for osteoid and bone in pig tibia (need to quantify > osteoid and bone volumes). Does anyone have experience with these types of > stains in 40-60 ?m thick, MMA embedded, sections? I have tried a Masson's > trichrome and Toluidine blue, but have been unable to get well defined stain > differentiation with this thick of sections. I would really appreciate any > help I can get. Thanks again. > > Jim > > > > ------------------------------ > > Message: 9 > Date: Wed, 17 Sep 2008 10:29:04 -0500 > From: "Paul Verden" > Subject: [Histonet] stain > To: > Message-ID: > > > Content-Type: text/plain; charset="US-ASCII" > > I am interested in finding the Alternate Bielschowsky Stain Protocol. > There are problems getting the silver to clear. > > > > > > > > ------------------------------ > > Message: 10 > Date: Wed, 17 Sep 2008 10:38:58 -0500 > From: "Rittman, Barry R" > Subject: RE: [Histonet] Question on staining > To: "Herrick, James L." , > > Message-ID: > < > EA1FDD2A141B7448B4B1AFFFCAC08DE40C048465@UTHEVS1.mail.uthouston.edu> > Content-Type: text/plain; charset="iso-8859-1" > > There is a question on the validity of measuring osteoid on thick sections. > The reason is that it is rare in bone to have sections where edges are > absolutely vertical throughout the section. You therefore have several > planes superimposed and several edges in these planes resulting in a > penumbra effect. The greater the angle to the vertical edge of the bone such > as edges of trabeculae or Haversian canals, the greater the errors in > measuring. > The most accurate measurements are those utilizing sections that are 5 > microns or below. > Is there some particular reason that you must use sections this thick? > Barry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. > Sent: Tuesday, September 16, 2008 5:12 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question on staining > > Hi all, > > I am trying to stain for osteoid and bone in pig tibia (need to quantify > osteoid and bone volumes). Does anyone have experience with these types of > stains in 40-60 ?m thick, MMA embedded, sections? I have tried a Masson's > trichrome and Toluidine blue, but have been unable to get well defined stain > differentiation with this thick of sections. I would really appreciate any > help I can get. Thanks again. > > Jim > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 11 > Date: Wed, 17 Sep 2008 10:50:47 -0500 > From: anita dudley > Subject: [Histonet] job opening > To: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > sorry I failed to put anything in the subject line on the previous note. > anita job is in fairhope ala. mohs lab in need of a histologist. > 251-633-1422 > _________________________________________________________________ > Get more out of the Web. Learn 10 hidden secrets of Windows Live. > > http://windowslive.com/connect/post/jamiethomson.spaces.live.com-Blog-cns!550F681DAD532637!5295.entry?ocid=TXT_TAGLM_WL_domore_092008 > > ------------------------------ > > Message: 12 > Date: Wed, 17 Sep 2008 10:58:01 -0500 > From: "Ben Spirto" > Subject: Re: [Histonet] stain > To: "Paul Verden" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <287d127c0809170858g53d73752v6cb4152870407ff1@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > *Bielschowski Stain* > > > *20% Silver Nitrate Solution:* > 1. Silver Nitrate=20g > 2. dWater=100ml > > > *Ammonia Water:* > 1. dWater=100ml > 2. Ammonium Hydroxide=8 drops > > > *Developer:* > 1. dWater=100ml > 2. Formalin=20ml > 3. Citric Acid=0.5g > 4. Concentrated Nitric Acid=2 drops > *Carcinogenic* > > *5%Sodium Thiosulfate (Hypo)* > 1. Sodium Thiosulfate=5g > 2.dWater=100ml > > > Ammonium Silver Solution > 1. 20% silver nitrate=100ml > 2. Ammonium Hydroxide=10ml > > > Protocol: > > On the stir plate to the 20% silver nitrate solution add 10-15 ml of > ammonium hydroxide, until brown precipitate forms, adding one drop at the > time. Keep adding more of ammonium hydroxide until solution is clear (while > vigorously stirring). Add couple drops of 20 % of silver nitrate until > solution is gold. Slides are placed in the ammonium silver solution in the > dark for 15-20 min. After that slides are placed in ammonia water. To the > ammonium silver solution add the developing solution (developer) in the > ratio of 8 drops of developer per 100ml of solution while stirring. Slides > are developed 3-5 min > > On Wed, Sep 17, 2008 at 10:29 AM, Paul Verden >wrote: > > > I am interested in finding the Alternate Bielschowsky Stain Protocol. > > There are problems getting the silver to clear. > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 13 > Date: Wed, 17 Sep 2008 16:08:21 +0000 > From: "Jack Ratliff " > Subject: RE: [Histonet] Question on staining > To: "Barry.R.Rittman@uth.tmc.edu " , > "Herrick.James@mayo.edu " , > "histonet@lists.utsouthwestern.edu " > > Message-ID: > Content-Type: text/plain; charset="iso-8859-15" > > Jim, > > I would like to respond to your question regarding the pig tibia. After > reading Barry's reply, I too question why you would not want to cut thin (5 > micron) sections. It then occurred to me that maybe you are not able to do > so because this would require the use of a sledge or polycut microtome. > > Given the size of your specimen, I agree that you you will have better and > more consistent results with your histomorphometry endpoints if you cut > thinner sections using a Reicher-Jung Polycut or a Leica SM2500. Upon > completion, you can then deplastify the sections (if you use MMA) and yield > excellent staining results by employing a Von Kossa reaction with a MacNeals > tetrachrome counterstain and/or a standard Goldners trichrome stain. > > If you need further assistance or more information, please feel free to > contact me > > Jack Ratliff > > -----Original Message----- > From: Rittman Barry R > Sent: Wednesday, September 17, 2008 11:39 AM > To: Herrick.James@mayo.edu; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Question on staining > > There is a question on the validity of measuring osteoid on thick sections. > The reason is that it is rare in bone to have sections where edges are > absolutely vertical throughout the section. You therefore have several > planes superimposed and several edges in these planes resulting in a > penumbra effect. The greater the angle to the vertical edge of the bone such > as edges of trabeculae or Haversian canals, the greater the errors in > measuring. > The most accurate measurements are those utilizing sections that are 5 > microns or below. > Is there some particular reason that you must use sections this thick? > Barry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. > Sent: Tuesday, September 16, 2008 5:12 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question on staining > > Hi all, > > I am trying to stain for osteoid and bone in pig tibia (need to quantify > osteoid and bone volumes). Does anyone have experience with these types of > stains in 40-60 ?m thick, MMA embedded, sections? I have tried a Masson's > trichrome and Toluidine blue, but have been unable to get well defined stain > differentiation with this thick of sections. I would really appreciate any > help I can get. Thanks again. > > Jim > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 58, Issue 20 > **************************************** > From dmlaud <@t> gmail.com Wed Sep 17 13:35:52 2008 From: dmlaud <@t> gmail.com (Damien) Date: Wed Sep 17 13:36:03 2008 Subject: [Histonet] Re: Osteoid staining Message-ID: <6f5a847e0809171135j1d5835f1pc4d59c3a8e0a5d6f@mail.gmail.com> Hi Jim, Does your study require Osteoid measurements in the transverse plane (you did not mention this)? If so, obviously you will need to use ground sections (30-40 microns). If your samples have not been processed, I would recommend doing an osteochrome bulk stain (the Villanueva method) stain and take your measurements using fluorescence. (Osteoid will be a vivid orange). Even if you've already processed, there are still options for using thick sections. Assuming your polishing technique is consistent (section thickness), you won't have any analysis problems. It is perfectly acceptable to use thick sections for osteoid measurement. -Damien Laudier On Wed, Sep 17, 2008 at 1:01 PM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Looking for H&E schedule for Varistain 24-4 (Randall Carpenter) > 2. Re: (no subject) (Geoff McAuliffe) > 3. (no subject) (anita dudley) > 4. Re: Amyloid (Maxim_71@mail.ru) > 5. RE: Amyloid (Mark A Burton) > 6. NSH class (Amber McKenzie) > 7. Permanent histo job in Austin, TX (Kyla Nemitz) > 8. Question on staining (Herrick, James L.) > 9. stain (Paul Verden) > 10. RE: Question on staining (Rittman, Barry R) > 11. job opening (anita dudley) > 12. Re: stain (Ben Spirto) > 13. RE: Question on staining (Jack Ratliff ) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 16 Sep 2008 10:45:39 -0700 (GMT-07:00) > From: Randall Carpenter > Subject: [Histonet] Looking for H&E schedule for Varistain 24-4 > To: Histonet > Message-ID: > < > 16648622.1221587139951.JavaMail.root@elwamui-royal.atl.sa.earthlink.net> > > Content-Type: text/plain; charset=UTF-8 > > Greetings Histonet, > > It's been a while since I was here and now I'm back. I'm now Twin Cities > Histology and it's a busy business right now. I just purchased a used > Varistain > 24-4 and was wondering if anyone had a good schedule for the Harris > Hematoxylin > (regressive). I've been doing the AFP method by hand for the last year or > so, > but now I'm up against it and had to buy the machine. Any help would be > appreciated. > Thanks. > > Randy Carpenter > Twin Cities Histology > > > > ------------------------------ > > Message: 2 > Date: Tue, 16 Sep 2008 14:17:48 -0400 > From: Geoff McAuliffe > Subject: Re: [Histonet] (no subject) > To: "TOJO (Torben Seested Johansen)" > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: <48CFF84C.2060502@umdnj.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Dear Tojo: > > Your problem could be due to 3 things. 1. Waiting too long to fix > the tissue. 2. Freezing too slowly. 3. Interpreting the lack of staining > of hepatocyte glycogen as an artifact. > > 1. You are waiting 5 minutes after death to fix the tissue. There is > absolutely NO reason to spend 5 min pumping 100 ml of PBS through the > circ. system. You will never wash out all of the blood and there is no > need to. AND when you finally get to fixative, the flow of fixative is > too low. Here is the solution to your problem. > > Use a 16 g needle in the L. vent. > Pump 10-15 ml of room temperature PBS into the rat in 15-20 seconds. > Pump 300 ml of room temperatue fix in 5 minutes. The fix is 4% > paraformaldehyde for light microscopy. For transmission EM the fix is 2 > or 3 or 4% paraformaldehyde with 1% glutaraldehyde. Buffer can be > either phosphate or cacodylate. The advantage of cac. buffer is that you > can add some calcium salts to stabilize membranes for EM (2milleMole) > without percipitation.Yes, you can add Ca salts to phos. buffer but it > will precipitate instantly (look up the solubility of CaPhosphate, or > should I say the insolubility). Of course, cacodylate has arsenic in it > so don't lick your fingers. > Remove liver and cut into appropriate sized pieces. For light microscopy > fix as long as possible, formalin reacts with tissue very slowly. > Glutaraldehyde fixed much faster, an hour or two is plenty. > Cryoprotect with sucrose. > 2. Tissue must be frozen VERY QUICKLY with 2-methylbutane (isopentane) > cooled with dry ice or liquid nitrogen otherwise you will have large > holes due to ice crystal formation. > 3. The stain you are using, Tol.Blue will not stain glycogen so you may > have empty-looking areas because of this. Use Periodic acid Schiff for > glycogen but NOT after glutaraldehyde fixation. > > Geoff > > > TOJO (Torben Seested Johansen) wrote: > > Hi, > > I do not seem to be able to achieve acceptble morphology of perfused rat > liver. I seems as if some of the cytosol of the hepatocytes are "washed > away" as seen in toluidine blue stained cryo-sections (see image18.jpg). I > am to use the tissue cryo-sections in immunohistochemistry and transmission > electron microscopy. > > > > I my attempts to get an acceptable/good morphology I have tried fixation > buffers consisting of 2, 4, 6 or 8 % paraformaldehyde (w/wo 0.1% > glutaraldehyde). > > > > My fixing procedure is as follows (all at room temperature); > > > > a rat (~250g) is anasthestized and a perfusion needle is inserted into > the left ventricle. The atrium is cut and PBS is flushed thru the rat at > 20ml/min for 5min. The rat is thereafter fixated at 10ml/min for 10 min with > fixation buffer (I have so far tried 2, 4, 6 or 8 % paraformaldehyde (with > and with out 0.1% glutaraldehyde) in 0.1M cacodylatebuffer (pH 7.4) all with > same unacceptable result (see image18.jpg). The liver is cut into smaller > pieces (~2*2*2mm) post fixed for 1h in the respective fixative, transferred > into 2.3M sucrose for minimum 1h (preferably over night) and frozen in > liquid nitrogen. > > > > Any idea why teh cytosol of my hepatocytes seems to be "gone" (hydropic > degeneration?) ? > > > > I have searched the net and it seems as if theres a 1000's of different > ways of performing rat liver perfusion fixation. Some use cold buffers and > others also perfuse with sucrose solution (either in combination with the > fixative or with sucrose alone post-fixation) > > > > what can I do to try and optimise my perfusion ? > > > > ______________________________________ > > > > Torben Seested Johansen > > Post Doc > > Exploratory ADME, Biopharmaceuticals > > > > Novo Nordisk A/S > > Novo Nordisk Park > > E9.S.22 > > DK-2760 M?l?v > > Denmark > > +45 44 43 14 84 (direct) > > +45 44 66 39 39 (fax) > > tojo@novonordisk.com > > www.novonordisk.com > > > > This e-mail (including any attachments) is intended for the addressee(s) > stated above only and may contain confidential information protected by law. > You are hereby notified that any unauthorized reading, disclosure, copying > or distribution of this e-mail or use of information contained herein is > strictly prohibited and may violate rights to proprietary information. If > you are not an intended recipient, please return this e-mail to the sender > and delete it immediately hereafter. Thank you. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 16 Sep 2008 13:52:42 -0500 > From: anita dudley > Subject: [Histonet] (no subject) > To: "histonet@pathology.swmed.edu" > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > job in fairhope alabama, beautiful area along the bay. dematologist office > looking for someone to do the mohs surgery. contact anita, providence hosp. > mobile alabama, 251-633-1422. > _________________________________________________________________ > See how Windows connects the people, information, and fun that are part of > your life. > http://clk.atdmt.com/MRT/go/msnnkwxp1020093175mrt/direct/01/ > > ------------------------------ > > Message: 4 > Date: Tue, 16 Sep 2008 23:05:58 +0400 > From: Maxim_71@mail.ru > Subject: Re: [Histonet] Amyloid > To: Dorothy.L.Webb@HealthPartners.Com > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <596251904.20080916230558@mail.ru> > Content-Type: text/plain; charset=us-ascii > > Dorothy: > We does Highman's method (Bancroft&Stevens, 1977 p.164) > Both 8 and 10 microns give good results. > Maxim Peshkov > Russia, > Taganrog. > mailto:Maxim_71@mail.ru > > > > > ------------------------------ > > Message: 5 > Date: Tue, 16 Sep 2008 15:31:30 -0400 > From: "Mark A Burton" > Subject: RE: [Histonet] Amyloid > To: "'Webb, Dorothy L'" , > > Message-ID: <000c01c91832$d24f65e0$76ee31a0$@edu> > Content-Type: text/plain; charset="US-ASCII" > > Dorothy, > > We've done several comparisons like this for amyloid staining. In our > experience, 8um works fine. We did see stronger staining (birefringence) in > some thicker sections with Congo Red but I don't think there would be a > significant improvement in staining (sensitivity?) from 8um to 10um. I > wouldn't cut sections any thinner than 8um but you can still get amyloid > staining at 4um. It would be relatively easy to take your controls and cut > them different thicknesses just to prove the point. Good luck! > > > Mark A Burton HTL ASCP > Lab Manager & Sr. Histotechnologist > Molecular Aging & Development Lab > Boston University Medical Campus > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, > Dorothy L > Sent: Tuesday, September 16, 2008 11:17 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Amyloid > > We have been cutting our tissue for amyloid staining @ 8 microns. One > of my pathologists heard that 10 microns is now standard and to use a > negative and weakly positive control. Does anyone have any new > information in this area? Thanks ahead of time! > > Dorothy Webb, HT (ASCP) > Histology Technical Supervisor > Regions Hospital, Pathology Department > 640 Jackson Street, Saint Paul, MN 55101-2595 > Phone: 651-254-2962 > Fax: 651-254-2741 > Regions Hospital is part of the HealthPartners family of care > ________________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 6 > Date: Tue, 16 Sep 2008 14:32:08 -0500 > From: "Amber McKenzie" > Subject: [Histonet] NSH class > To: > Message-ID: > <03C921A1EAF7F541B16543F6EC6A4B3701EB6B95@giamail2.Gia.com> > Content-Type: text/plain; charset="us-ascii" > > Hi! I was wondering if anyone was going to NSH and attending the class > for "Are you Ready for the HT board of Registry Exam"? by speaker: > Robert L Lott, BS, HTL (ASCP), Trinity Medial Center/LabFirst, Inc., > Birmingham, AL ? I am not able to attend NSH this year, and I'm really > interested in the material that Robert will be presenting about. I am > already HT certified, but I want to take the HTL soon and thought this > class would be a good start for study material. I emailed Pebbles at NSH > for the speakers email address to contact him personally, but I never > heard back from her. If anyone is planning on going to this class, > could you send me a copy of any handouts? > > > > Thanks, > > Amber McKenzie, B.S., HT (ASCP) > > 1405 N. State St., Suite 400 > > Jackson, MS 39202 > > (ph) 601-863-0388 > > (fax) 601-326-3532 > > > > > > > > ------------------------------ > > Message: 7 > Date: Tue, 16 Sep 2008 16:24:29 -0400 > From: "Kyla Nemitz" > Subject: [Histonet] Permanent histo job in Austin, TX > To: > Message-ID: > <9416D9FA37C1C04FA83D69684E95D2E71E4DDD95@exbk2.maxhealth.com> > Content-Type: text/plain; charset="us-ascii" > > Hello all - > > > > I am looking for an ASCP Histo tech for a permanent position in Austin, > TX. The position is day shift, M-F and pays $55-60K/year. The facility > is also helping with relocation. > > > > If you or anyone you know is currently looking to relocation to Texas, > please contact me. > > > > Also - I specialize in placing histo techs on travel and permanent > positions Nationwide. If you have any questions or are looking for a > position, please feel free to call or email. > > > > Thank you in advance, your help is appreciated! > > > > Kyla Nemitz > > TravelMax Medical Professionals - Maxim Healthcare > > * 888.800.1855 or 813.371.5175 > > 7 800.294.1248 > > www.TravelMaxAllied.com < > http://www.travelmaxallied.com/> > > > > > > ------------------------------ > > Message: 8 > Date: Tue, 16 Sep 2008 17:11:46 -0500 > From: "Herrick, James L." > Subject: [Histonet] Question on staining > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Hi all, > > I am trying to stain for osteoid and bone in pig tibia (need to quantify > osteoid and bone volumes). Does anyone have experience with these types of > stains in 40-60 ?m thick, MMA embedded, sections? I have tried a Masson's > trichrome and Toluidine blue, but have been unable to get well defined stain > differentiation with this thick of sections. I would really appreciate any > help I can get. Thanks again. > > Jim > > > > ------------------------------ > > Message: 9 > Date: Wed, 17 Sep 2008 10:29:04 -0500 > From: "Paul Verden" > Subject: [Histonet] stain > To: > Message-ID: > > > Content-Type: text/plain; charset="US-ASCII" > > I am interested in finding the Alternate Bielschowsky Stain Protocol. > There are problems getting the silver to clear. > > > > > > > > ------------------------------ > > Message: 10 > Date: Wed, 17 Sep 2008 10:38:58 -0500 > From: "Rittman, Barry R" > Subject: RE: [Histonet] Question on staining > To: "Herrick, James L." , > > Message-ID: > < > EA1FDD2A141B7448B4B1AFFFCAC08DE40C048465@UTHEVS1.mail.uthouston.edu> > Content-Type: text/plain; charset="iso-8859-1" > > There is a question on the validity of measuring osteoid on thick sections. > The reason is that it is rare in bone to have sections where edges are > absolutely vertical throughout the section. You therefore have several > planes superimposed and several edges in these planes resulting in a > penumbra effect. The greater the angle to the vertical edge of the bone such > as edges of trabeculae or Haversian canals, the greater the errors in > measuring. > The most accurate measurements are those utilizing sections that are 5 > microns or below. > Is there some particular reason that you must use sections this thick? > Barry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. > Sent: Tuesday, September 16, 2008 5:12 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question on staining > > Hi all, > > I am trying to stain for osteoid and bone in pig tibia (need to quantify > osteoid and bone volumes). Does anyone have experience with these types of > stains in 40-60 ?m thick, MMA embedded, sections? I have tried a Masson's > trichrome and Toluidine blue, but have been unable to get well defined stain > differentiation with this thick of sections. I would really appreciate any > help I can get. Thanks again. > > Jim > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 11 > Date: Wed, 17 Sep 2008 10:50:47 -0500 > From: anita dudley > Subject: [Histonet] job opening > To: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > sorry I failed to put anything in the subject line on the previous note. > anita job is in fairhope ala. mohs lab in need of a histologist. > 251-633-1422 > _________________________________________________________________ > Get more out of the Web. Learn 10 hidden secrets of Windows Live. > > http://windowslive.com/connect/post/jamiethomson.spaces.live.com-Blog-cns!550F681DAD532637!5295.entry?ocid=TXT_TAGLM_WL_domore_092008 > > ------------------------------ > > Message: 12 > Date: Wed, 17 Sep 2008 10:58:01 -0500 > From: "Ben Spirto" > Subject: Re: [Histonet] stain > To: "Paul Verden" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <287d127c0809170858g53d73752v6cb4152870407ff1@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > *Bielschowski Stain* > > > *20% Silver Nitrate Solution:* > 1. Silver Nitrate=20g > 2. dWater=100ml > > > *Ammonia Water:* > 1. dWater=100ml > 2. Ammonium Hydroxide=8 drops > > > *Developer:* > 1. dWater=100ml > 2. Formalin=20ml > 3. Citric Acid=0.5g > 4. Concentrated Nitric Acid=2 drops > *Carcinogenic* > > *5%Sodium Thiosulfate (Hypo)* > 1. Sodium Thiosulfate=5g > 2.dWater=100ml > > > Ammonium Silver Solution > 1. 20% silver nitrate=100ml > 2. Ammonium Hydroxide=10ml > > > Protocol: > > On the stir plate to the 20% silver nitrate solution add 10-15 ml of > ammonium hydroxide, until brown precipitate forms, adding one drop at the > time. Keep adding more of ammonium hydroxide until solution is clear (while > vigorously stirring). Add couple drops of 20 % of silver nitrate until > solution is gold. Slides are placed in the ammonium silver solution in the > dark for 15-20 min. After that slides are placed in ammonia water. To the > ammonium silver solution add the developing solution (developer) in the > ratio of 8 drops of developer per 100ml of solution while stirring. Slides > are developed 3-5 min > > On Wed, Sep 17, 2008 at 10:29 AM, Paul Verden >wrote: > > > I am interested in finding the Alternate Bielschowsky Stain Protocol. > > There are problems getting the silver to clear. > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 13 > Date: Wed, 17 Sep 2008 16:08:21 +0000 > From: "Jack Ratliff " > Subject: RE: [Histonet] Question on staining > To: "Barry.R.Rittman@uth.tmc.edu " , > "Herrick.James@mayo.edu " , > "histonet@lists.utsouthwestern.edu " > > Message-ID: > Content-Type: text/plain; charset="iso-8859-15" > > Jim, > > I would like to respond to your question regarding the pig tibia. After > reading Barry's reply, I too question why you would not want to cut thin (5 > micron) sections. It then occurred to me that maybe you are not able to do > so because this would require the use of a sledge or polycut microtome. > > Given the size of your specimen, I agree that you you will have better and > more consistent results with your histomorphometry endpoints if you cut > thinner sections using a Reicher-Jung Polycut or a Leica SM2500. Upon > completion, you can then deplastify the sections (if you use MMA) and yield > excellent staining results by employing a Von Kossa reaction with a MacNeals > tetrachrome counterstain and/or a standard Goldners trichrome stain. > > If you need further assistance or more information, please feel free to > contact me > > Jack Ratliff > > -----Original Message----- > From: Rittman Barry R > Sent: Wednesday, September 17, 2008 11:39 AM > To: Herrick.James@mayo.edu; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Question on staining > > There is a question on the validity of measuring osteoid on thick sections. > The reason is that it is rare in bone to have sections where edges are > absolutely vertical throughout the section. You therefore have several > planes superimposed and several edges in these planes resulting in a > penumbra effect. The greater the angle to the vertical edge of the bone such > as edges of trabeculae or Haversian canals, the greater the errors in > measuring. > The most accurate measurements are those utilizing sections that are 5 > microns or below. > Is there some particular reason that you must use sections this thick? > Barry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. > Sent: Tuesday, September 16, 2008 5:12 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question on staining > > Hi all, > > I am trying to stain for osteoid and bone in pig tibia (need to quantify > osteoid and bone volumes). Does anyone have experience with these types of > stains in 40-60 ?m thick, MMA embedded, sections? I have tried a Masson's > trichrome and Toluidine blue, but have been unable to get well defined stain > differentiation with this thick of sections. I would really appreciate any > help I can get. Thanks again. > > Jim > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 58, Issue 20 > **************************************** > From rjbuesa <@t> yahoo.com Wed Sep 17 14:06:52 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 17 14:06:56 2008 Subject: [Histonet] Helicobacter In-Reply-To: <000a01c918ec$5a178890$06570181@sfmc.net> Message-ID: <987538.12532.qm@web65705.mail.ac4.yahoo.com> We always used positive cases, selected by our pathologists. Ren? J. --- On Wed, 9/17/08, Matt Roark wrote: From: Matt Roark Subject: [Histonet] Helicobacter To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 17, 2008, 1:39 PM What is everyone using for a Helicobacter control (non-ihc)? We are currently buying our Helico control slides from Master Tech and using the Diff Quick method to stain them. Our pathologist is not very happy with the controls so we need to find a different source. Any help would be appreciated! Thanks!!! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Sep 17 15:10:35 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Sep 17 15:10:39 2008 Subject: [Histonet] Malloys hematoxylin Message-ID: Does anyone have a protocol on how to make up malloys hematoxylin. I have tried the web and did not come up with much. Any help is appreciated and thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 From JWeems <@t> sjha.org Wed Sep 17 15:13:55 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Sep 17 15:14:12 2008 Subject: [Histonet] Malloys hematoxylin In-Reply-To: References: Message-ID: <982A0A9461F9BF438C7B19A6E425A383637100@ITSSSXM01V6.one.ads.che.org> Would it be Mallory's instead of Malloy maybe? There's plenty of info on that... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, September 17, 2008 4:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Malloys hematoxylin Does anyone have a protocol on how to make up malloys hematoxylin. I have tried the web and did not come up with much. Any help is appreciated and thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From mpence <@t> grhs.net Wed Sep 17 15:41:07 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Sep 17 15:41:13 2008 Subject: [Histonet] Helicobacter In-Reply-To: <000a01c918ec$5a178890$06570181@sfmc.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3906@IS-E2K3.grhs.net> We take a + case from our micro dept and the plate it off for us. We grow 4-6 plates and then swab the growth off and suspend it in broth. The broth is sectioned, processed and placed in block. This will last us for a couple years at a time. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Roark Sent: Wednesday, September 17, 2008 12:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter What is everyone using for a Helicobacter control (non-ihc)? We are currently buying our Helico control slides from Master Tech and using the Diff Quick method to stain them. Our pathologist is not very happy with the controls so we need to find a different source. Any help would be appreciated! Thanks!!! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Wed Sep 17 17:33:18 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Sep 17 17:33:23 2008 Subject: [Histonet] PSLIM Printer Message-ID: <48D185AE.9010906@pathology.washington.edu> A quick update. We just started playing with it and right out of the box it does not print 2D barcodes. It will print an image of whatever you send it. Our IT dept is creating a driver for it. It might be plug and play depending upon what application you are working with. Getting the print to line up correctly needs some work and it doesn't seem as fast as expected. It sounds like the Microwriter cassette printer with the stylus only quieter. I'll keep you posted Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From marktarango <@t> gmail.com Thu Sep 18 00:23:08 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Sep 18 00:23:15 2008 Subject: [Histonet] EGFP IHC in floating brain sections In-Reply-To: References: Message-ID: <5b6eb13e0809172223r5bf0c527o59aa4f2ecd75badb@mail.gmail.com> Hi Caroline, I didn't see an avidin/biotin block but noticed that your donkey anti-goat has biotin as its label. Do you think that a blocking step might clear up the background on the ones w/ the strong signal (because of endogenous biotin in the brain getting stained)? Or maybe it would be easier to clean up the staining if you tried something in the range of 1:600- 1:1000 for that biotin labeled seconary. Just some thoughts, Mark On Wed, Sep 17, 2008 at 11:12 AM, Caroline Bass wrote: > Hello, > > Sorry if this is a double post but I didn't see my first one in the digest. > > I?m hoping someone could help me troubleshoot my staining protocol. I am > staining to EGFP in microglia (produced by a virus I injected in the > brain). > Here?s my general protocol: > > Deactive endogenous peroxidases (0.1M PB + 20% methanol + titon-x100 (80 ul > in 40 ml total) + 0.3% h2O2) for 15 min. > Wash 3X15min in 0.1MPB+0.3%titon > Incubate in 0.1MPB+0.3%triton+1%normal donkey serum for 1 hr > Incubate with primary (goat anti-GFP from Rockland) overnight at 4 degrees > (1:1000) > Wash 3x15 min 0.1M PB + 0.3% triton > Incubate in with secondary (Jackson Donkey anti-goat, biotin labeled) > overnight at 4 degrees (1:500) > Wash 3x15 min in 0.1M PB > > Use ABC kit and vector SG for substrate (can?t remember how long I let it > develop, but it was within a couple of minutes). > > So, the bottom line is that I did a test section and I thought it had a lot > of background, so I incubated the other sections for less time. However, > when I took a closer look, the sections with little background had no > microglial signal, while the one with high background had a very clear and > noticeable signal in the place it should be. So, the question is how to cut > back on the noise. Would increasing the secondary concentration to 1:1000 > help? I?m following a protocol someone else came up with, but it seems > strange that the secondary is at a higher concentration than the primary. > > Thanks! > > Caroline > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From marktarango <@t> gmail.com Thu Sep 18 00:34:16 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Sep 18 00:34:22 2008 Subject: [Histonet] EGFP IHC in floating brain sections In-Reply-To: <5b6eb13e0809172223r5bf0c527o59aa4f2ecd75badb@mail.gmail.com> References: <5b6eb13e0809172223r5bf0c527o59aa4f2ecd75badb@mail.gmail.com> Message-ID: <5b6eb13e0809172234w43ae07e1tb08ced0284151888@mail.gmail.com> I just realized that you suggested going to 1:1000 for that antibody but you said "increasing the concentration." Going from 1:500 to 1:100 is diluting it out further... anyway I do think that should help, if it doest... again maybe an avidin/biotin blocking kit would help. Mark On Wed, Sep 17, 2008 at 10:23 PM, Mark Tarango wrote: > Hi Caroline, > > I didn't see an avidin/biotin block but noticed that your donkey anti-goat > has biotin as its label. Do you think that a blocking step might clear up > the background on the ones w/ the strong signal (because of endogenous > biotin in the brain getting stained)? > > Or maybe it would be easier to clean up the staining if you tried something > in the range of 1:600- 1:1000 for that biotin labeled seconary. > > Just some thoughts, > > Mark > > On Wed, Sep 17, 2008 at 11:12 AM, Caroline Bass wrote: > >> Hello, >> >> Sorry if this is a double post but I didn't see my first one in the >> digest. >> >> I?m hoping someone could help me troubleshoot my staining protocol. I am >> staining to EGFP in microglia (produced by a virus I injected in the >> brain). >> Here?s my general protocol: >> >> Deactive endogenous peroxidases (0.1M PB + 20% methanol + titon-x100 (80 >> ul >> in 40 ml total) + 0.3% h2O2) for 15 min. >> Wash 3X15min in 0.1MPB+0.3%titon >> Incubate in 0.1MPB+0.3%triton+1%normal donkey serum for 1 hr >> Incubate with primary (goat anti-GFP from Rockland) overnight at 4 degrees >> (1:1000) >> Wash 3x15 min 0.1M PB + 0.3% triton >> Incubate in with secondary (Jackson Donkey anti-goat, biotin labeled) >> overnight at 4 degrees (1:500) >> Wash 3x15 min in 0.1M PB >> >> Use ABC kit and vector SG for substrate (can?t remember how long I let it >> develop, but it was within a couple of minutes). >> >> So, the bottom line is that I did a test section and I thought it had a >> lot >> of background, so I incubated the other sections for less time. However, >> when I took a closer look, the sections with little background had no >> microglial signal, while the one with high background had a very clear and >> noticeable signal in the place it should be. So, the question is how to >> cut >> back on the noise. Would increasing the secondary concentration to 1:1000 >> help? I?m following a protocol someone else came up with, but it seems >> strange that the secondary is at a higher concentration than the primary. >> >> Thanks! >> >> Caroline >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From davew <@t> uic.edu Thu Sep 18 01:07:57 2008 From: davew <@t> uic.edu (David Wirtshafter) Date: Thu Sep 18 01:08:05 2008 Subject: [Histonet] EGFP IHC in floating brain sections In-Reply-To: <200809180537.m8I5bHff021861@mailin-16.priv.cc.uic.edu> References: <200809180537.m8I5bHff021861@mailin-16.priv.cc.uic.edu> Message-ID: <200809180607.m8I67vPQ005429@mail-4.priv.cc.uic.edu> >Caroline , There are a lot of things you could try, but I'd recommend, as a first shot, that you omit the Trition from the rinses after the primary and from the secondary solution. I don't understand exactly why, and it's certainly not intuitive, but for quite a few primaries, including Triton at these steps can dramatically increase background staining. I'd be surprised if changing the secondary concentration improved things, but it's quite possible that reducing the primary concentration might have a beneficial effect. Dave >Message: 3 >Date: Wed, 17 Sep 2008 14:12:19 -0400 >From: Caroline Bass >Subject: >To: >Message-ID: >Content-Type: text/plain; charset="ISO-8859-1" > >Hello, > >Sorry if this is a double post but I didn't see my first one in the digest. > >I?m hoping someone could help me troubleshoot my staining protocol. I am >staining to EGFP in microglia (produced by a virus I injected in the brain). >Here?s my general protocol: > >Deactive endogenous peroxidases (0.1M PB + 20% methanol + titon-x100 (80 ul >in 40 ml total) + 0.3% h2O2) for 15 min. >Wash 3X15min in 0.1MPB+0.3%titon >Incubate in 0.1MPB+0.3%triton+1%normal donkey serum for 1 hr >Incubate with primary (goat anti-GFP from Rockland) overnight at 4 degrees >(1:1000) >Wash 3x15 min 0.1M PB + 0.3% triton >Incubate in with secondary (Jackson Donkey anti-goat, biotin labeled) >overnight at 4 degrees (1:500) >Wash 3x15 min in 0.1M PB > >Use ABC kit and vector SG for substrate (can?t remember how long I let it >develop, but it was within a couple of minutes). > >So, the bottom line is that I did a test section and I thought it had a lot >of background, so I incubated the other sections for less time. However, >when I took a closer look, the sections with little background had no >microglial signal, while the one with high background had a very clear and >noticeable signal in the place it should be. So, the question is how to cut >back on the noise. Would increasing the secondary concentration to 1:1000 >help? I?m following a protocol someone else came up with, but it seems >strange that the secondary is at a higher concentration than the primary. > >Thanks! > >Caroline > > Dr. David Wirtshafter Laboratory of Integrative Neuroscience Department of Psychology, m/c 285 University of Illinois at Chicago Room 1009 Behavioral Sciences Building 1007 W. Harrison St. Chicago, IL 60607-7137 From C.J.Milligan <@t> leeds.ac.uk Thu Sep 18 02:59:40 2008 From: C.J.Milligan <@t> leeds.ac.uk (Carol Milligan) Date: Thu Sep 18 03:00:06 2008 Subject: [Histonet] mouse monocytes isolation Message-ID: <705BBF854F6EFD4AB1B8E2217AACB8B15B4EB6@HERMES1.ds.leeds.ac.uk> I would like to make some electrophysiological recordings from mouse monocytes using an automated planar patchclamp system. The problem is, that I need at least 1x106cells /ml to do this. I have never isolated monocytes from mice so I would like to locate a lab in the UK who routinely isolate pure preparations of mouse monocytes so that I can arrange to visit to learn the technique first hand. Can anybody help me in the matter? Carol From bgv <@t> sun.ac.za Thu Sep 18 05:20:34 2008 From: bgv <@t> sun.ac.za (Versfeld, BG, Mr ) Date: Thu Sep 18 05:22:07 2008 Subject: [Histonet] RE: Histonet Digest, Vol 58, Issue 20 References: Message-ID: <2E07007E881F8340B11F94C8773E51568B6172@TYGEVS01.tyg.sun.ac.za> can someone please provide me with a recipe for hirschprung's disease. thanx. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of histonet-request@lists.utsouthwestern.edu Sent: Wed 2008/09/17 07:06 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 58, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Looking for H&E schedule for Varistain 24-4 (Randall Carpenter) 2. Re: (no subject) (Geoff McAuliffe) 3. (no subject) (anita dudley) 4. Re: Amyloid (Maxim_71@mail.ru) 5. RE: Amyloid (Mark A Burton) 6. NSH class (Amber McKenzie) 7. Permanent histo job in Austin, TX (Kyla Nemitz) 8. Question on staining (Herrick, James L.) 9. stain (Paul Verden) 10. RE: Question on staining (Rittman, Barry R) 11. job opening (anita dudley) 12. Re: stain (Ben Spirto) 13. RE: Question on staining (Jack Ratliff ) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Sep 2008 10:45:39 -0700 (GMT-07:00) From: Randall Carpenter Subject: [Histonet] Looking for H&E schedule for Varistain 24-4 To: Histonet Message-ID: <16648622.1221587139951.JavaMail.root@elwamui-royal.atl.sa.earthlink.net> Content-Type: text/plain; charset=UTF-8 Greetings Histonet, It's been a while since I was here and now I'm back. I'm now Twin Cities Histology and it's a busy business right now. I just purchased a used Varistain 24-4 and was wondering if anyone had a good schedule for the Harris Hematoxylin (regressive). I've been doing the AFP method by hand for the last year or so, but now I'm up against it and had to buy the machine. Any help would be appreciated. Thanks. Randy Carpenter Twin Cities Histology ------------------------------ Message: 2 Date: Tue, 16 Sep 2008 14:17:48 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] (no subject) To: "TOJO (Torben Seested Johansen)" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <48CFF84C.2060502@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Dear Tojo: Your problem could be due to 3 things. 1. Waiting too long to fix the tissue. 2. Freezing too slowly. 3. Interpreting the lack of staining of hepatocyte glycogen as an artifact. 1. You are waiting 5 minutes after death to fix the tissue. There is absolutely NO reason to spend 5 min pumping 100 ml of PBS through the circ. system. You will never wash out all of the blood and there is no need to. AND when you finally get to fixative, the flow of fixative is too low. Here is the solution to your problem. Use a 16 g needle in the L. vent. Pump 10-15 ml of room temperature PBS into the rat in 15-20 seconds. Pump 300 ml of room temperatue fix in 5 minutes. The fix is 4% paraformaldehyde for light microscopy. For transmission EM the fix is 2 or 3 or 4% paraformaldehyde with 1% glutaraldehyde. Buffer can be either phosphate or cacodylate. The advantage of cac. buffer is that you can add some calcium salts to stabilize membranes for EM (2milleMole) without percipitation.Yes, you can add Ca salts to phos. buffer but it will precipitate instantly (look up the solubility of CaPhosphate, or should I say the insolubility). Of course, cacodylate has arsenic in it so don't lick your fingers. Remove liver and cut into appropriate sized pieces. For light microscopy fix as long as possible, formalin reacts with tissue very slowly. Glutaraldehyde fixed much faster, an hour or two is plenty. Cryoprotect with sucrose. 2. Tissue must be frozen VERY QUICKLY with 2-methylbutane (isopentane) cooled with dry ice or liquid nitrogen otherwise you will have large holes due to ice crystal formation. 3. The stain you are using, Tol.Blue will not stain glycogen so you may have empty-looking areas because of this. Use Periodic acid Schiff for glycogen but NOT after glutaraldehyde fixation. Geoff TOJO (Torben Seested Johansen) wrote: > Hi, > I do not seem to be able to achieve acceptble morphology of perfused rat liver. I seems as if some of the cytosol of the hepatocytes are "washed away" as seen in toluidine blue stained cryo-sections (see image18.jpg). I am to use the tissue cryo-sections in immunohistochemistry and transmission electron microscopy. > > I my attempts to get an acceptable/good morphology I have tried fixation buffers consisting of 2, 4, 6 or 8 % paraformaldehyde (w/wo 0.1% glutaraldehyde). > > My fixing procedure is as follows (all at room temperature); > > a rat (~250g) is anasthestized and a perfusion needle is inserted into the left ventricle. The atrium is cut and PBS is flushed thru the rat at 20ml/min for 5min. The rat is thereafter fixated at 10ml/min for 10 min with fixation buffer (I have so far tried 2, 4, 6 or 8 % paraformaldehyde (with and with out 0.1% glutaraldehyde) in 0.1M cacodylatebuffer (pH 7.4) all with same unacceptable result (see image18.jpg). The liver is cut into smaller pieces (~2*2*2mm) post fixed for 1h in the respective fixative, transferred into 2.3M sucrose for minimum 1h (preferably over night) and frozen in liquid nitrogen. > > Any idea why teh cytosol of my hepatocytes seems to be "gone" (hydropic degeneration?) ? > > I have searched the net and it seems as if theres a 1000's of different ways of performing rat liver perfusion fixation. Some use cold buffers and others also perfuse with sucrose solution (either in combination with the fixative or with sucrose alone post-fixation) > > what can I do to try and optimise my perfusion ? > > ______________________________________ > > Torben Seested Johansen > Post Doc > Exploratory ADME, Biopharmaceuticals > > Novo Nordisk A/S > Novo Nordisk Park > E9.S.22 > DK-2760 M?l?v > Denmark > +45 44 43 14 84 (direct) > +45 44 66 39 39 (fax) > tojo@novonordisk.com > www.novonordisk.com > > This e-mail (including any attachments) is intended for the addressee(s) stated above only and may contain confidential information protected by law. You are hereby notified that any unauthorized reading, disclosure, copying or distribution of this e-mail or use of information contained herein is strictly prohibited and may violate rights to proprietary information. If you are not an intended recipient, please return this e-mail to the sender and delete it immediately hereafter. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 3 Date: Tue, 16 Sep 2008 13:52:42 -0500 From: anita dudley Subject: [Histonet] (no subject) To: "histonet@pathology.swmed.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" job in fairhope alabama, beautiful area along the bay. dematologist office looking for someone to do the mohs surgery. contact anita, providence hosp. mobile alabama, 251-633-1422. _________________________________________________________________ See how Windows connects the people, information, and fun that are part of your life. http://clk.atdmt.com/MRT/go/msnnkwxp1020093175mrt/direct/01/ ------------------------------ Message: 4 Date: Tue, 16 Sep 2008 23:05:58 +0400 From: Maxim_71@mail.ru Subject: Re: [Histonet] Amyloid To: Dorothy.L.Webb@HealthPartners.Com Cc: histonet@lists.utsouthwestern.edu Message-ID: <596251904.20080916230558@mail.ru> Content-Type: text/plain; charset=us-ascii Dorothy: We does Highman's method (Bancroft&Stevens, 1977 p.164) Both 8 and 10 microns give good results. Maxim Peshkov Russia, Taganrog. mailto:Maxim_71@mail.ru ------------------------------ Message: 5 Date: Tue, 16 Sep 2008 15:31:30 -0400 From: "Mark A Burton" Subject: RE: [Histonet] Amyloid To: "'Webb, Dorothy L'" , Message-ID: <000c01c91832$d24f65e0$76ee31a0$@edu> Content-Type: text/plain; charset="US-ASCII" Dorothy, We've done several comparisons like this for amyloid staining. In our experience, 8um works fine. We did see stronger staining (birefringence) in some thicker sections with Congo Red but I don't think there would be a significant improvement in staining (sensitivity?) from 8um to 10um. I wouldn't cut sections any thinner than 8um but you can still get amyloid staining at 4um. It would be relatively easy to take your controls and cut them different thicknesses just to prove the point. Good luck! Mark A Burton HTL ASCP Lab Manager & Sr. Histotechnologist Molecular Aging & Development Lab Boston University Medical Campus -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, September 16, 2008 11:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amyloid We have been cutting our tissue for amyloid staining @ 8 microns. One of my pathologists heard that 10 microns is now standard and to use a negative and weakly positive control. Does anyone have any new information in this area? Thanks ahead of time! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 16 Sep 2008 14:32:08 -0500 From: "Amber McKenzie" Subject: [Histonet] NSH class To: Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701EB6B95@giamail2.Gia.com> Content-Type: text/plain; charset="us-ascii" Hi! I was wondering if anyone was going to NSH and attending the class for "Are you Ready for the HT board of Registry Exam"? by speaker: Robert L Lott, BS, HTL (ASCP), Trinity Medial Center/LabFirst, Inc., Birmingham, AL ? I am not able to attend NSH this year, and I'm really interested in the material that Robert will be presenting about. I am already HT certified, but I want to take the HTL soon and thought this class would be a good start for study material. I emailed Pebbles at NSH for the speakers email address to contact him personally, but I never heard back from her. If anyone is planning on going to this class, could you send me a copy of any handouts? Thanks, Amber McKenzie, B.S., HT (ASCP) 1405 N. State St., Suite 400 Jackson, MS 39202 (ph) 601-863-0388 (fax) 601-326-3532 ------------------------------ Message: 7 Date: Tue, 16 Sep 2008 16:24:29 -0400 From: "Kyla Nemitz" Subject: [Histonet] Permanent histo job in Austin, TX To: Message-ID: <9416D9FA37C1C04FA83D69684E95D2E71E4DDD95@exbk2.maxhealth.com> Content-Type: text/plain; charset="us-ascii" Hello all - I am looking for an ASCP Histo tech for a permanent position in Austin, TX. The position is day shift, M-F and pays $55-60K/year. The facility is also helping with relocation. If you or anyone you know is currently looking to relocation to Texas, please contact me. Also - I specialize in placing histo techs on travel and permanent positions Nationwide. If you have any questions or are looking for a position, please feel free to call or email. Thank you in advance, your help is appreciated! Kyla Nemitz TravelMax Medical Professionals - Maxim Healthcare * 888.800.1855 or 813.371.5175 7 800.294.1248 www.TravelMaxAllied.com ------------------------------ Message: 8 Date: Tue, 16 Sep 2008 17:11:46 -0500 From: "Herrick, James L." Subject: [Histonet] Question on staining To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi all, I am trying to stain for osteoid and bone in pig tibia (need to quantify osteoid and bone volumes). Does anyone have experience with these types of stains in 40-60 ?m thick, MMA embedded, sections? I have tried a Masson's trichrome and Toluidine blue, but have been unable to get well defined stain differentiation with this thick of sections. I would really appreciate any help I can get. Thanks again. Jim ------------------------------ Message: 9 Date: Wed, 17 Sep 2008 10:29:04 -0500 From: "Paul Verden" Subject: [Histonet] stain To: Message-ID: Content-Type: text/plain; charset="US-ASCII" I am interested in finding the Alternate Bielschowsky Stain Protocol. There are problems getting the silver to clear. ------------------------------ Message: 10 Date: Wed, 17 Sep 2008 10:38:58 -0500 From: "Rittman, Barry R" Subject: RE: [Histonet] Question on staining To: "Herrick, James L." , Message-ID: Content-Type: text/plain; charset="iso-8859-1" There is a question on the validity of measuring osteoid on thick sections. The reason is that it is rare in bone to have sections where edges are absolutely vertical throughout the section. You therefore have several planes superimposed and several edges in these planes resulting in a penumbra effect. The greater the angle to the vertical edge of the bone such as edges of trabeculae or Haversian canals, the greater the errors in measuring. The most accurate measurements are those utilizing sections that are 5 microns or below. Is there some particular reason that you must use sections this thick? Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. Sent: Tuesday, September 16, 2008 5:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question on staining Hi all, I am trying to stain for osteoid and bone in pig tibia (need to quantify osteoid and bone volumes). Does anyone have experience with these types of stains in 40-60 ?m thick, MMA embedded, sections? I have tried a Masson's trichrome and Toluidine blue, but have been unable to get well defined stain differentiation with this thick of sections. I would really appreciate any help I can get. Thanks again. Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 17 Sep 2008 10:50:47 -0500 From: anita dudley Subject: [Histonet] job opening To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" sorry I failed to put anything in the subject line on the previous note. anita job is in fairhope ala. mohs lab in need of a histologist. 251-633-1422 _________________________________________________________________ Get more out of the Web. Learn 10 hidden secrets of Windows Live. http://windowslive.com/connect/post/jamiethomson.spaces.live.com-Blog-cns!550F681DAD532637!5295.entry?ocid=TXT_TAGLM_WL_domore_092008 ------------------------------ Message: 12 Date: Wed, 17 Sep 2008 10:58:01 -0500 From: "Ben Spirto" Subject: Re: [Histonet] stain To: "Paul Verden" Cc: histonet@lists.utsouthwestern.edu Message-ID: <287d127c0809170858g53d73752v6cb4152870407ff1@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 *Bielschowski Stain* *20% Silver Nitrate Solution:* 1. Silver Nitrate=20g 2. dWater=100ml *Ammonia Water:* 1. dWater=100ml 2. Ammonium Hydroxide=8 drops *Developer:* 1. dWater=100ml 2. Formalin=20ml 3. Citric Acid=0.5g 4. Concentrated Nitric Acid=2 drops *Carcinogenic* *5%Sodium Thiosulfate (Hypo)* 1. Sodium Thiosulfate=5g 2.dWater=100ml Ammonium Silver Solution 1. 20% silver nitrate=100ml 2. Ammonium Hydroxide=10ml Protocol: On the stir plate to the 20% silver nitrate solution add 10-15 ml of ammonium hydroxide, until brown precipitate forms, adding one drop at the time. Keep adding more of ammonium hydroxide until solution is clear (while vigorously stirring). Add couple drops of 20 % of silver nitrate until solution is gold. Slides are placed in the ammonium silver solution in the dark for 15-20 min. After that slides are placed in ammonia water. To the ammonium silver solution add the developing solution (developer) in the ratio of 8 drops of developer per 100ml of solution while stirring. Slides are developed 3-5 min On Wed, Sep 17, 2008 at 10:29 AM, Paul Verden wrote: > I am interested in finding the Alternate Bielschowsky Stain Protocol. > There are problems getting the silver to clear. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Wed, 17 Sep 2008 16:08:21 +0000 From: "Jack Ratliff " Subject: RE: [Histonet] Question on staining To: "Barry.R.Rittman@uth.tmc.edu " , "Herrick.James@mayo.edu " , "histonet@lists.utsouthwestern.edu " Message-ID: Content-Type: text/plain; charset="iso-8859-15" Jim, I would like to respond to your question regarding the pig tibia. After reading Barry's reply, I too question why you would not want to cut thin (5 micron) sections. It then occurred to me that maybe you are not able to do so because this would require the use of a sledge or polycut microtome. Given the size of your specimen, I agree that you you will have better and more consistent results with your histomorphometry endpoints if you cut thinner sections using a Reicher-Jung Polycut or a Leica SM2500. Upon completion, you can then deplastify the sections (if you use MMA) and yield excellent staining results by employing a Von Kossa reaction with a MacNeals tetrachrome counterstain and/or a standard Goldners trichrome stain. If you need further assistance or more information, please feel free to contact me Jack Ratliff -----Original Message----- From: Rittman Barry R Sent: Wednesday, September 17, 2008 11:39 AM To: Herrick.James@mayo.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Question on staining There is a question on the validity of measuring osteoid on thick sections. The reason is that it is rare in bone to have sections where edges are absolutely vertical throughout the section. You therefore have several planes superimposed and several edges in these planes resulting in a penumbra effect. The greater the angle to the vertical edge of the bone such as edges of trabeculae or Haversian canals, the greater the errors in measuring. The most accurate measurements are those utilizing sections that are 5 microns or below. Is there some particular reason that you must use sections this thick? Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. Sent: Tuesday, September 16, 2008 5:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question on staining Hi all, I am trying to stain for osteoid and bone in pig tibia (need to quantify osteoid and bone volumes). Does anyone have experience with these types of stains in 40-60 ?m thick, MMA embedded, sections? I have tried a Masson's trichrome and Toluidine blue, but have been unable to get well defined stain differentiation with this thick of sections. I would really appreciate any help I can get. Thanks again. Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 58, Issue 20 **************************************** From Beverly.Rowe <@t> easternhealth.ca Thu Sep 18 06:09:18 2008 From: Beverly.Rowe <@t> easternhealth.ca (Beverly Rowe) Date: Thu Sep 18 06:09:29 2008 Subject: [Histonet] ?bacterial/fungal contamination on slides Message-ID: We are experiencing contamination of GMS and PAS-F stained slides with a filamentous microorganism, bacterial or possibly fungal. Our flotation baths and stainers are cleaned daily with Sparkleen and water. We use distilled water to fill the baths. We use the Ventana system for the stains, which is decontaminated regularly. Anyone one else experience this? Any suggestions to the cause of this. Thanks, Beverly Rowe, RT. Pathology Quality Management Coordinator Pathology Dept.Rm.2423 St.Clare's Mercy Hospital Eastern Health St. John's, NL 709-777-5658 From RSRICHMOND <@t> aol.com Thu Sep 18 08:49:57 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Sep 18 08:50:06 2008 Subject: [Histonet] Re: Helicobacter stain control Message-ID: Since gastric biopsy specimens with abundant Helicobacter are rather common, it's easy to find Helicobacter controls among your own specimens, and almost every laboratory I've worked in has obtained controls out of their own material. When I see a case that would make a good control (not every positive case is suitable) I write a note on a slip of paper "S08-xxxx good Helicobacter control" and give it to the responsible histotechnologist. Fortunately gastrectomy specimens for acute ulcer disease have become rare, but if you ever get one, you can get enough Helicobacter controls to last you forever, so be on the lookout for such specimens. I don't think making controls from bacterial cultures would be very satisfactory. For one thing, Helicobacter pylori is extremely difficult to culture. I frequently see Helicobacter controls that don't contain any bugs, and histologically don't look like they should contain any (no neutrophils present). If the dye stain looks satisfactory, I sign it out, with the words "a suitable control slide" rather than "a positive control slide" in my microscopic note. I don't do this in labs that do IHC - there the control has to be positive. Bob Richmond Samurai Pathologist Knoxville TN From mroark <@t> sfmc.net Thu Sep 18 09:46:46 2008 From: mroark <@t> sfmc.net (Matt Roark) Date: Thu Sep 18 09:47:13 2008 Subject: [Histonet] Helicobacter References: <661949901A768E4F9CC16D8AF8F2838C017A3906@IS-E2K3.grhs.net> Message-ID: <004501c9199d$62a52600$06570181@sfmc.net> Thanks everyone! I also thought that we should be using positive patient controls but our pathologist wasn't to keen on the idea for some reason. But now with all of your responses maybe we will be. Thanks again! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 From TMcNemar <@t> lmhealth.org Thu Sep 18 10:00:30 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Sep 18 10:00:44 2008 Subject: [Histonet] ?bacterial/fungal contamination on slides In-Reply-To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5B7@lmhsmail.lmhealth.org> We had a similar problem awhile back and traced it to our precut control slides. We cut certain control slides ahead and store them in slide boxes. We started seeing fungus where it should not have been. We were just cutting the control slides, putting the wet slides into the boxes, covering the box, and then (as if that wasn't enough) we stored them over the embedding station. We think that the combination of moist, dark, and warm slides made the perfect environment for fungus. We got rid of those slides and boxes and have since allowed the slides to dry completely before putting them away. No more contamination.... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Beverly Rowe Sent: Thursday, September 18, 2008 7:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ?bacterial/fungal contamination on slides We are experiencing contamination of GMS and PAS-F stained slides with a filamentous microorganism, bacterial or possibly fungal. Our flotation baths and stainers are cleaned daily with Sparkleen and water. We use distilled water to fill the baths. We use the Ventana system for the stains, which is decontaminated regularly. Anyone one else experience this? Any suggestions to the cause of this. Thanks, Beverly Rowe, RT. Pathology Quality Management Coordinator Pathology Dept.Rm.2423 St.Clare's Mercy Hospital Eastern Health St. John's, NL 709-777-5658 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu Sep 18 10:43:12 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Sep 18 10:43:17 2008 Subject: [Histonet] ?bacterial/fungal contamination on slides Message-ID: <57BE698966D5C54EAE8612E8941D768303BD438B@EXCHANGE3.huntingtonhospital.com> We see this every once in a while, and have traced it to the water stations on our stainer. They are supposed to be cleaned with bleach once a week, but that wasn't happening. Another time, we had contamination on the spigot of our 5 gallon deionized water bottle. We are supposed to clean that with bleach quarterly. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beverly Rowe Sent: Thursday, September 18, 2008 4:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ?bacterial/fungal contamination on slides We are experiencing contamination of GMS and PAS-F stained slides with a filamentous microorganism, bacterial or possibly fungal. Our flotation baths and stainers are cleaned daily with Sparkleen and water. We use distilled water to fill the baths. We use the Ventana system for the stains, which is decontaminated regularly. Anyone one else experience this? Any suggestions to the cause of this. Thanks, Beverly Rowe, RT. Pathology Quality Management Coordinator Pathology Dept.Rm.2423 St.Clare's Mercy Hospital Eastern Health St. John's, NL 709-777-5658 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Sep 18 10:51:48 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 18 10:51:51 2008 Subject: [Histonet] Helicobacter In-Reply-To: <004501c9199d$62a52600$06570181@sfmc.net> Message-ID: <389222.3480.qm@web65713.mail.ac4.yahoo.com> The reason why?the pathologists are usually reluctant to use (+) cases, is because if they are used as a (+) controls?they will be?probably used up totally within a short period of time and IF there is some sort of legal action against them or the hospital in one of those cases there will be no a block to return to and make additional sections for litigation purposes, and it is an understandable position. Ren? J. --- On Thu, 9/18/08, Matt Roark wrote: From: Matt Roark Subject: Re: [Histonet] Helicobacter To: histonet@lists.utsouthwestern.edu Date: Thursday, September 18, 2008, 10:46 AM Thanks everyone! I also thought that we should be using positive patient controls but our pathologist wasn't to keen on the idea for some reason. But now with all of your responses maybe we will be. Thanks again! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Thu Sep 18 11:08:30 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Sep 18 11:08:30 2008 Subject: [Histonet] Helicobacter In-Reply-To: <389222.3480.qm@web65713.mail.ac4.yahoo.com> Message-ID: We used to cut just a number of slides from each positive block that we had. This way no block was exhausted in the process. Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 09/18/2008 08:55 AM Please respond to rjbuesa@yahoo.com To histonet@lists.utsouthwestern.edu, Matt Roark cc Subject Re: [Histonet] Helicobacter The reason why the pathologists are usually reluctant to use (+) cases, is because if they are used as a (+) controls they will be probably used up totally within a short period of time and IF there is some sort of legal action against them or the hospital in one of those cases there will be no a block to return to and make additional sections for litigation purposes, and it is an understandable position. Ren? J. --- On Thu, 9/18/08, Matt Roark wrote: From: Matt Roark Subject: Re: [Histonet] Helicobacter To: histonet@lists.utsouthwestern.edu Date: Thursday, September 18, 2008, 10:46 AM Thanks everyone! I also thought that we should be using positive patient controls but our pathologist wasn't to keen on the idea for some reason. But now with all of your responses maybe we will be. Thanks again! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robinsoc <@t> mercyhealth.com Thu Sep 18 11:26:33 2008 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Thu Sep 18 11:26:56 2008 Subject: [Histonet] Helicobacter In-Reply-To: <389222.3480.qm@web65713.mail.ac4.yahoo.com> References: <004501c9199d$62a52600$06570181@sfmc.net> <389222.3480.qm@web65713.mail.ac4.yahoo.com> Message-ID: <48D23AE9.59AC.00AF.0@mercyhealth.com> We keep track of our positive cases and only cut one ribbon for use as a positive control. This way we don't use up the block. Cindi >>> Rene J Buesa 09/18/2008 10:51 AM >>> The reason why the pathologists are usually reluctant to use (+) cases, is because if they are used as a (+) controls they will be probably used up totally within a short period of time and IF there is some sort of legal action against them or the hospital in one of those cases there will be no a block to return to and make additional sections for litigation purposes, and it is an understandable position. Ren? J. --- On Thu, 9/18/08, Matt Roark wrote: From: Matt Roark Subject: Re: [Histonet] Helicobacter To: histonet@lists.utsouthwestern.edu Date: Thursday, September 18, 2008, 10:46 AM Thanks everyone! I also thought that we should be using positive patient controls but our pathologist wasn't to keen on the idea for some reason. But now with all of your responses maybe we will be. Thanks again! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu Sep 18 14:18:02 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Sep 18 14:18:07 2008 Subject: [Histonet] Ventana Retic Stain Message-ID: <57BE698966D5C54EAE8612E8941D768303BD4484@EXCHANGE3.huntingtonhospital.com> Is anyone out there having problems with their Retic stain on the Ventana Nexus Special Stainer? Please contact me offline. Laurie Colbert From histoinfo <@t> comcast.net Thu Sep 18 14:47:34 2008 From: histoinfo <@t> comcast.net (histoinfo@comcast.net) Date: Thu Sep 18 14:47:39 2008 Subject: [Histonet] Cassette Holders Message-ID: <091820081947.12914.48D2B056000CBE5500003272221652585601000207019B9C0708@comcast.net> Greetings Netters, For those of you that remember the old metal cassette holders. It seems that they are no longer available (no surprise), and I cant find anything like them on the market. They were sold by tissue-tek I think. They are about 4 inches square and one inch tall. They have 5 rows that hold 3 cassettes each for a total of 15 cassettes. We are looking for more of these, if anyone has some they are no longer using please contact me. Many Thanks, Jennifer Sauners HT (ASCP) From kynemitz <@t> travmax.com Thu Sep 18 17:20:52 2008 From: kynemitz <@t> travmax.com (Kyla Nemitz) Date: Thu Sep 18 17:21:14 2008 Subject: [Histonet] Travel/Temp position in Beautiful Orange County, California Message-ID: <9416D9FA37C1C04FA83D69684E95D2E71E4DDE7F@exbk2.maxhealth.com> Hello All - One of the facilities I work closely with has asked me to search for a travel histo tech. A facility in Orange County, CA needs a contract ASCP histo tech for a 13 week travel assignment starting somewhat as soon as possible. I specialize in placing histo techs on travel and permanent positions Nationwide. If you have any questions or are looking for a position, please feel free to call or email. I can be reached via email kynemitz@travmax.com or 8-6 EST 888-800-1855 ext. 5175 Thank you! Kyla Nemitz TravelMax Medical Professionals - Maxim Healthcare Tel: 888.800.1855 or 813.371.5175 Fax: 800.294.1248 www.TravelMaxAllied.com From jhabecke <@t> fhcrc.org Thu Sep 18 17:36:34 2008 From: jhabecke <@t> fhcrc.org (Randolph-Habecker, Julie) Date: Thu Sep 18 17:36:43 2008 Subject: [Histonet] Humidity levels in the lab Message-ID: <040346FA7309BD439C327F97D4C4D69B04A1C06C@ISIS.fhcrc.org> Folks, We just moved to a new lab space in a new building. We are currently running at 45% or lower humidity. I am working with our facilities on raising the level but I am looking for some references for what level we should aim for in regard to paraffin cutting. Does anyone have some ideas or places to look for information? Thanks!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org From joost.bruijntjes <@t> tno.nl Fri Sep 19 02:23:57 2008 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Fri Sep 19 02:24:13 2008 Subject: [Histonet] FW: BrdU Message-ID: <8865601DD17A554CB489C17FFD8A51B2019C8D53@MAIL04.tsn.tno.nl> Thanks to those with an answer, problem is solved. That means that one of the pretreatment steps (pronase) stopped working. Changing to another batch pronase was enough to see BrdU staining in both rats and mice Thanks agian Joost ________________________________ From: Bruijntjes, J.P. (Joost) Sent: donderdag 11 september 2008 12:04 To: histonet@lists.utsouthwestern.edu Subject: BrdU Hi Histonetters I do have a problem with BrdU, and I hope someone of you can give a kind of solution/explanation. I work with NALT (Nose Associated Lymphoid Tissue) of both rats and mice, which were fixed in a fixative composed of acetic acid, formaldehyde, ethanol and aqua dest. After 48 hours this fixative was replaced by ethanol. Later on the tissues were dehydrated and embedded in paraffin. Just because the lymphoid tissues material is so small, about 10 paraffin slides were collected. I started with the rat NALT's. One paraffin slide was stained with HE, and a few other slides were stained with a monoclonal antibody directed against BrdU. I was satisfied with the staining, so far no problem. After the rats, the same story with the mice NALT's. But I did not get any positive staining. Some days later I repeated the first try-out included with some positive controls from the rat-study with the primary antibody I used in the first part, and the biotin conjugated primary antibody as well, but again no positive staining. The pre-treatment of the slides for rat and mice is the same. The only difference lies in the primary and secondary antibody. For rat tissue I use a non-conjugated monoclonal antibody, followed by HRP-conjugated powervision. For mice tissue I use a biotin conjugated monoclonal antibody, followed by a HRP-conjugated streptavidin. Can anyone give me an explanation? The storage of the slides was in a room with an equal temperature (about 20-21?C) and humidity. Is it possible that the epitopes in the single slides are destroyed so that they are not recognizable anymore by the antibody? The time between preparing the slides and the BrdU staining of the rats NALT slides was at the most 4 weeks, while the first negative staining on the mice NALT's appeared after about 8 weeks. Thanks in advance Joost Bruijntjes TNO Quality of Life Zeist The Netherlands TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From mwich <@t> 7thwavelabs.com Fri Sep 19 08:09:26 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Fri Sep 19 08:09:37 2008 Subject: [Histonet] immuno diluent vs. buffer question Message-ID: <62A8156F8071C8439080D626DF8C33A602E4C1@wave-mail.7thwave.local> I have a question for those doing immunos by hand. I typically dilute my primary, secondary and tertiary antibodies with manufacturer supplied diluent. Recently, I was running a test to determine the source of a mysterious precipitate that had begun to appear on my slides (It has since then disappeared just as mysteriously). In one of the conditions, I substituted the diluent with TBST for all dilutions. I found that this particular slide had noticeably less background. When inquiring as to what I should be diluting these reagents with, I received conflicting answers from tech support. I heard that the secondary and tertiary antibodies should be diluted with what they are actually in, i.e. .05% TBS or 1M PBS. I also was told that I could dilute everything with diluent. Can anyone tell me which is correct? Or why using the buffer yielded less background than the diluent? Aside from sodium azide and BSA I don't know what's in the proprietary reagent. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From Susan.Weber2 <@t> va.gov Fri Sep 19 08:15:34 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Fri Sep 19 08:15:41 2008 Subject: [Histonet] ?bacterial/fungal contamination on slides In-Reply-To: References: Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76E52@VHAV10MSGA1.v10.med.va.gov> Have you checked your water source for contamination? We culture our DI water regularly. Make sure you are culturing from the tap you actually obtain the water from. We had this problem at another hospital a few years ago and found contamination at the tap. Hope this helps. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beverly Rowe Sent: Thursday, September 18, 2008 7:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ?bacterial/fungal contamination on slides We are experiencing contamination of GMS and PAS-F stained slides with a filamentous microorganism, bacterial or possibly fungal. Our flotation baths and stainers are cleaned daily with Sparkleen and water. We use distilled water to fill the baths. We use the Ventana system for the stains, which is decontaminated regularly. Anyone one else experience this? Any suggestions to the cause of this. Thanks, Beverly Rowe, RT. Pathology Quality Management Coordinator Pathology Dept.Rm.2423 St.Clare's Mercy Hospital Eastern Health St. John's, NL 709-777-5658 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Fri Sep 19 08:36:33 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Sep 19 08:36:53 2008 Subject: [Histonet] barcode labels vs handwritten Message-ID: <48D372A1.2B7F.00C9.0@geisinger.edu> Hi Folks, I am running into resistance with a few of our pathologists regarding the use of barcoded labels. They want us to handwrite our slides and then apply our permanent labels over top of that. We are using the Stainershield labels that are solvent-resistant. We eliminated the step of relabeling our slides with the permanent labels at the sorting area, and began having the cutter label their slides with the permanent labels at the microtome area as part of a Lean workflow initiative. However, a few of our Pathologists like having the handwriting underneath the label so they can see who cut the slide if it is of poor quality. Also, if they think it is mislabeled they say that gives them a clue as to where the slide really came from. I guess I feel like, if the slide is mislabeled it doesn't matter where it came from, it still needs to be recut from the correct block. I'd appreciate your comments and suggestions about this issue. Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From RossS <@t> BaylorHealth.edu Fri Sep 19 08:47:07 2008 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 19 08:47:12 2008 Subject: [Histonet] barcode labels vs handwritten In-Reply-To: <48D372A1.2B7F.00C9.0@geisinger.edu> Message-ID: I'm very interested in this subject. I've thought of using this approach for labeling slides. However, I am nervous about the labels staying on years down the line. Also there is the double check of the handwriting. There are 2 checks of identity when handwriting first, but there are also 2 opportunities to mislabel the slide. What is best practice with only using barcoded labels and not handwriting? Is the practice widespread enough that there is a consensus. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, September 19, 2008 8:37 AM To: histonet Subject: [Histonet] barcode labels vs handwritten Hi Folks, I am running into resistance with a few of our pathologists regarding the use of barcoded labels. They want us to handwrite our slides and then apply our permanent labels over top of that. We are using the Stainershield labels that are solvent-resistant. We eliminated the step of relabeling our slides with the permanent labels at the sorting area, and began having the cutter label their slides with the permanent labels at the microtome area as part of a Lean workflow initiative. However, a few of our Pathologists like having the handwriting underneath the label so they can see who cut the slide if it is of poor quality. Also, if they think it is mislabeled they say that gives them a clue as to where the slide really came from. I guess I feel like, if the slide is mislabeled it doesn't matter where it came from, it still needs to be recut from the correct block. I'd appreciate your comments and suggestions about this issue. Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From RSRICHMOND <@t> aol.com Fri Sep 19 09:16:25 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Sep 19 09:16:29 2008 Subject: [Histonet] Re: Amyloid Message-ID: I think I've posted to Histonet on this subject before, with no response - about amyloid controls. Amyloid control tissue is extremely hard to get, since amyloidosis is quite a rare disease, and very few autopsies are performed these days. Controls I've seen lately are usually from surgically resected medullary thyroid carcinomas, but this is a rare disease also, and something of an off-brand amyloid (formed from calcitonin) is present in only about 25% of them. The need to cut fresh sections every time means that blocks are quickly exhausted. Amyloidosis of the AA type is rather easily induced in mice - see http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2765 for a little information and a brief bibliography (I have no connection with Sigma-Aldrich). I would think that this mouse tissue containing amyloid could be used as a control. Does anyone use it? Bob Richmond Samurai Pathologist Knoxville TN From PMonfils <@t> Lifespan.org Fri Sep 19 09:30:21 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Sep 19 09:30:42 2008 Subject: [Histonet] ?bacterial/fungal contamination on slides In-Reply-To: <16C83872A53F4346AA9C3A18E3A3AAB903F76E52@VHAV10MSGA1.v10.med.va.gov> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C5D@LSRIEXCH1.lsmaster.lifespan.org> Fungi can grow in a number of different aqueous stain solutions. One time a tech brought me a slide she had stained with GMS for fungi, together with the positive control slide she had run. I could see fungi on the test slide but there was no sign of silver staining. The control slide showed positively stained fungi as expected, but also the same unstained fungi seen on the test slide. It turned out a fungus was growing in the aqueous solution of Fast Green we used as a counterstain for the GMS. From JEllin <@t> yumaregional.org Fri Sep 19 09:41:44 2008 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Sep 19 09:42:10 2008 Subject: [Histonet] barcode labels vs handwritten References: Message-ID: <29BE166A2CF48D459853F8EC57CD37E8016111EC@EXCHANGECLUSTER.yumaregional.local> I understand the issues that you are going through at this time and how to go forward with this project,, I feel that barcoding is going to open new doors within the Histology field,, we will be able to fill the gaps and begin to enter a new area of process workflows and techniques within our departments and facilities,, The hardest thing is to choose the correct project or gradient for success,, We here at Yuma Regional went through several phases of barcoding, with applying lean concepts,, we came to discover alot of pros and cons on both sides of the coin,, but very little cons,, We started out using single "D" barcoding with paper labels as you all,, but noticed several issues with them,, 1. To make sure that you purchase quality lables and #2 That they can withstand the staining process,, but finding a vendor for labels was not difficult, and intially the labels staying on there was not a problem,, but over time when either there needs to be re slipping of the slide or slides stuck together within a drawer,, we found that no label no matter how much we were assured by the vendor they ended up failing,, there are also issue with coverslipping, at times the labels cause the cover slip to not sit right on the slide or if we labeled them after the staining process we were spending additional time,, so we ended up going to a slide writer that has reduced alot of time wasted on slide issue, it improved quality and it is dependable,, we also changedc to 2D barcodes will allow for additional programming for future project builds.. I would say that we as a field need to embrace this technology sooner than later and also look to the future at what this technology can bring,, if any one has any questions feel free email or call me. Jesus Ellin HT/PA ASCP Yuma Regional Medical Center Department of Pathology 928-336-1144 or 928-336-7444 jellin@yumaregional.org This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. From asachau <@t> titanmed.com Fri Sep 19 09:47:44 2008 From: asachau <@t> titanmed.com (April Sachau) Date: Fri Sep 19 09:48:05 2008 Subject: [Histonet] PA position available! In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8016111EC@EXCHANGECLUSTER.yumaregional.local> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F7017F510C@titansbs1.corp.titanmed.com> Hello! I have a temporary Pathologist's Assistant position available currently on the East Coast. This would be a 10-13 week assignment. Dayshift, Monday through Friday. All interested candidates contact me for details!!! Thanks! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From JWeems <@t> sjha.org Fri Sep 19 09:57:08 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Sep 19 09:57:12 2008 Subject: [Histonet] barcode labels vs handwritten In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8016111EC@EXCHANGECLUSTER.yumaregional.local> References: <29BE166A2CF48D459853F8EC57CD37E8016111EC@EXCHANGECLUSTER.yumaregional.local> Message-ID: <982A0A9461F9BF438C7B19A6E425A383637681@ITSSSXM01V6.one.ads.che.org> And we use barcodeing, even tho all of the end users do not. We'll get there eventually! This big of a change takes a while. We get labels from Printer's Plus and are very happy with them. They are very thin and very secure. We also now have a Sakura slide printer - that is interfaced with our LIS. We still write our initial on the slides. We have unique bar codes for the case, each specimen, each block, and each slide. If they are used properly, many chances for error are eliminated. Good luck to us all as we move into the future. Hope everyone is surviving the stock market challenge. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From victor <@t> pathology.washington.edu Fri Sep 19 10:00:04 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Sep 19 10:00:33 2008 Subject: [Histonet] barcode labels vs handwritten In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8016111EC@EXCHANGECLUSTER.yumaregional.local> References: <29BE166A2CF48D459853F8EC57CD37E8016111EC@EXCHANGECLUSTER.yumaregional.local> Message-ID: <48D3BE74.3050804@pathology.washington.edu> Accountability can be handled through computer software and workflow. When a tech is ready to cut a block, they scan the bar coded block and the labels or slides are printed. Who scanned and cut the block is captured in the LIS. Not all systems are there yet, but progress is moving rapidly in this direction. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Jesus Ellin wrote: > I understand the issues that you are going through at this time and how to go forward with this project,, I feel that barcoding is going to open new doors within the Histology field,, we will be able to fill the gaps and begin to enter a new area of process workflows and techniques within our departments and facilities,, The hardest thing is to choose the correct project or gradient for success,, We here at Yuma Regional went through several phases of barcoding, with applying lean concepts,, we came to discover alot of pros and cons on both sides of the coin,, but very little cons,, We started out using single "D" barcoding with paper labels as you all,, but noticed several issues with them,, 1. To make sure that you purchase quality lables and #2 That they can withstand the staining process,, but finding a vendor for labels was not difficult, and intially the labels staying on there was not a problem,, but over time when either there needs to be re slipping of the slide or slides stuck together within a drawer,, we found that no label no matter how much we were assured by the vendor they ended up failing,, there are also issue with coverslipping, at times the labels cause the cover slip to not sit right on the slide or if we labeled them after the staining process we were spending additional time,, so we ended up going to a slide writer that has reduced alot of time wasted on slide issue, it improved quality and it is dependable,, we also changedc to 2D barcodes will allow for additional programming for future project builds.. I would say that we as a field need to embrace this technology sooner than later and also look to the future at what this technology can bring,, if any one has any questions feel free email or call me. > > > Jesus Ellin HT/PA ASCP > Yuma Regional Medical Center > Department of Pathology > 928-336-1144 or 928-336-7444 > jellin@yumaregional.org > > > > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank You. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Shirley_PHUA <@t> hsa.gov.sg Fri Sep 19 13:03:01 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Fri Sep 19 13:04:57 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 19-09-2008 to 20-09-2008. I am on half day off on 19/09/2008 afternoon ... Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From tjasper <@t> copc.net Fri Sep 19 17:01:34 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri Sep 19 17:01:43 2008 Subject: [Histonet] Humidity levels in the lab References: <040346FA7309BD439C327F97D4C4D69B04A1C06C@ISIS.fhcrc.org> Message-ID: <90354A475B420441B2A0396E5008D496731768@copc-sbs.COPC.local> Julie, I can't offer you any references, but here's something to consider. A cool mist humidifier in your lab. We are in Bend, OR., on the dry side of the Cascades. Running the humidifier reduces static and makes sectioning a bit easier. No hard science with this, just a little more atmospheric moisture. By the way, the humidifier I bought is a big blue and white penguin. The mist streams out the beak. Just looking at it makes you smile. I bought it at Target for ~ $40.00 - it works for us. Have a good weekend. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Randolph-Habecker, Julie Sent: Thursday, September 18, 2008 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Humidity levels in the lab Folks, We just moved to a new lab space in a new building. We are currently running at 45% or lower humidity. I am working with our facilities on raising the level but I am looking for some references for what level we should aim for in regard to paraffin cutting. Does anyone have some ideas or places to look for information? Thanks!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Sat Sep 20 06:11:31 2008 From: tifei <@t> foxmail.com (tf) Date: Sat Sep 20 06:12:08 2008 Subject: [Histonet] Improving the BrdU staining References: <20080911191659.BEB59005@m4500-03.uchicago.edu> Message-ID: <200809201911259246894@foxmail.com> RGVhciBBbGw6DQoNCkkgYW0gd3JpdGluZyB0byBkaXNjdXNzIHRoZSBmdW5jdGlvbiBvZiBlYWNo IHN0ZXAgaW4gQnJkVSBzdGFpbmluZy4NCkFzIHlvdSBtaWdodCBrbm93LCBDaXRyYXRlIGJ1ZmZl ciByZXRyaWV2YWwgYXQgOTUgZGVncmVlIGZvciAzMCBtaW4sIGFuZCAyTiBIQ2wgZm9yIGFub3Ro ZXIgMzAgbWluIGF0IFJUIGFyZSB0d28gY3JpdGljYWwgc3RlcHMgZm9yIEJyZFUgc3RhaW5pbmcu DQoNCigxKSBGb3IgY2l0cmF0ZSBidWZmZXIgcmV0cmlldmFsOiBJcyB0aGlzIHJlYWxseSBhbnRp Z2VuIHJldHJpZXZhbD8gT3Igd2UgYXJlIGp1c3QgdXNpbmcgY2l0cmF0ZSBidWZmZXIgdG8gcG9y 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cGVhcmVkDQphZnRlciBhYm91dCA4IHdlZWtzLg0KVGhhbmtzIGluIGFkdmFuY2UNCkpvb3N0IEJy dWlqbnRqZXMNClROTyBRdWFsaXR5IG9mIExpZmUNClplaXN0DQpUaGUgTmV0aGVybGFuZHMNClRO Ty5OTCA8aHR0cDovL3d3dy50bm8ubmwvPg0KSm9vc3QgQnJ1aWpudGplcw0KVCArMzEgMzAgNjk0 IDQ0IDgwDQpGICszMSAzMCA2OTQgNDkgODYNCkUgam9vc3QuYnJ1aWpudGplc0B0bm8ubmwgPG1h aWx0bzpqb29zdC5icnVpam50amVzQHRuby5ubD4NCkRhdmlkIEEuIFdyaWdodCwgUGguRC4NClVu aXZlcnNpdHkgb2YgQ2hpY2Fnbw0KU2VjdGlvbiBvZiBOZXVyb3N1cmdlcnksIE1DMzAyNg0KNTg0 MSBTLiBNYXJ5bGFuZCBBdmUNCkNoaWNhZ28sIElMIDYwNjM3IFVTQQ0KW1ddIDc3My04MzQtMTQ4 NSAoT2ZmaWNlKSBTQlJJIFJtIEozMjgNCiAgICA3NzMtODM0LTAzOTUgKExhYikgU0JSSSBSbSBK MzM4DQpbSF0gNzczLTY0My02OTc2DQpQYWdlciAoQDc3My03NTMtMTg4MCkgIzk3NzYNClRleHQg cGFnZSAxMjAgY2hhciBFLW1haWwgNzczODQ1NjgzNEBteWFpcm1haWwuY29tDQpvciB3ZWI6IGh0 dHA6Ly93d3cubXlhaXJtYWlsLmNvbSB0byA3NzM4NDU2ODM0DQpGQVggKE5ldXJvc3VyZ2VyeSkg NzczLjcwMi4zNTE4DQpXZWIgRkFYICYgcGVyc29uYWwgdm9pY2VtYWlsOiAoODE1KTY0Mi05NjMw DQpTa3lwZTogZGF2aWRhbnRob255d3JpZ2h0DQogPT09PT09PT09PT09PT09PT09PT09PT09PT09 PT09PT09PT09PT09PT09PT09PT09DQpEb2VzIDIrMj01IGZvciBsYXJnZSB2YWx1ZXMgb2YgMj8N Cl9fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fDQpIaXN0b25l dCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdQ0KaHR0cDov L2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0DQo= From pruegg <@t> ihctech.net Sat Sep 20 12:29:35 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Sep 20 12:29:40 2008 Subject: [Histonet] Improving the BrdU staining In-Reply-To: <200809201911259246894@foxmail.com> Message-ID: Joost, You might want to join the NSH IHC Resource Group online at www.ihcrg.org we are a committee thru NSH of over 500 from all over the world working and sharing our expertise in IHC and Molecular Methods. We have a list serve similar to histonet that is focused just on IHC issues. Membership with NSH is the only requirement to be part of this resource group. If you are not a member apply for group membership online anyway and we will tell you how to join NSH. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tf Sent: Saturday, September 20, 2008 5:12 AM To: histonet@lists.utsouthwestern.ed Subject: [Histonet] Improving the BrdU staining Dear All: I am writing to discuss the function of each step in BrdU staining. As you might know, Citrate buffer retrieval at 95 degree for 30 min, and 2N HCl for another 30 min at RT are two critical steps for BrdU staining. (1) For citrate buffer retrieval: Is this really antigen retrieval? Or we are just using citrate buffer to porize the cell membrane? For antigen retrieval you should slowly cool down the slides in citrate buffer following heating - but I just took those slides out and put them in PBS, and go on with other steps- I can get quite good results. However if I slowly cool down the slides, I found some "dirty spots" between two sections on my slide - do anyone have such experiences? I read papers that some people using protease for this step in BrdU staining - I think that's also for porization on cell membrane. Thus, can we just use TBS (Tween or Tris??) to make pores on cell membrane before HCl treatment? What're the differences? (2) For HCl treatment: Do anyone has other suggestions on the use of concentration and time for HCl treatment so that the nucleus structure for DAPI staining wont be completely disrupted? The 2N HCl for 30 min at 37 degree really brings very weak DAPI staining then. (3) do anyone tried only Citrate buffer step or HCl step? Or you have other topics want to discuss here? Cheers! 2008-09-13 tf ???? David A. Wright ????? 2008-09-12 08:21:47 ???? histonet@lists.utsouthwestern.edu ??? ??? [Histonet] Re: BrdU Hi Joost & the Histonet I have some general comments on BrdU that i hope help. Maybe someone else has more specific ideas relating to your tissue too? It seems you are having problems with 2 things at once: a) switching species b) switching antibodies My guess is you only have control over the latter but either could be the problem! a) species - since DNA is DNA, you don't have to worry about species specificity of your antibody. Unless you are doing tissue culture, it's not however always simple to get the same BrdU incorporation in different species. I take it that someone else - possibly different people - labelled the mice and rats and also that it was done in vivo. Even repeat experiments on the same species are variable. The BrdU doesn't always go into solution equally (it needs a dilute base, not buffered saline) and it has to have a phosphate added on inside the cell before it is incorporated. So metabolic rate is important not just dose/kg. It's a good idea to collect control tissue from every animal to confirm incorporation - pick any abundant, proliferating tissue. I've often used gut. You then also have lots of material to work up and compare different antibodies. I'm probably not the only one who'll say you have to try your 2 antibodies side by side on the same specimen. b) Different antibodies. Hmmm. You mention fixation procedures but don't say much about what you did to recover antigenicity in your paraffin sections before staining. With BrdU you have to do something just to make it accessible. In Eukaryotes, (BrdU-containing) nuclear DNA is highly compacted into nucleosomes with lots of histones and other proteins all around it and then squashed down a whole lot more. Fixation will lock all these proteins together and make it hard for the Ab to find its target. I think it's a truism that all nuclear antigens, including proteins, need some kind of harsh treatment (heat, strong acid or base, proteinase) to make the nucleus accessible. It's interesting that your unconjugated MAb worked but not the larger conjugated one - maybe it's just penetration. On this point, you didn't say whether your protocol was exactly the same for each Ab. I've used a bunch of different anti-BrdU Abs and the manufacturers have quite different recommendations about pretreatment - 2N HCl, formamide, SSC, NaOH, proteinase. If you've only got a tiny bit of tissue, you MUST do exactly what the manufacturer says [at least the first time] even if that's different from what worked for you before with a different Ab (I'd do both!). It often helps NOT to block, so the Ab can hit the naked DNA, however exposed. [c) time. You mentioned different delays before processing. BrdU staining is one of the few things where that won't matter. As DNA doesn't degrade like proteins, antigenicity isn't lost with time.] Try Again! Since (same logic) the DNA will still be there on your slides, IF BrdU was actually incorporated (see a), there's a very high likelihood that your negative slides WILL stain with either a different antibody and/or harsher pretreatment as recommended by the manufacturer. I liked BD Bioscience's MAb (clone B44) which only needed a few minutes in 0.07N NaOH to unmask to BrdU. I think it's still available. Good luck! -David -------- David A. Wright PhD University of Chicago Section of Neurosurgery ---- Original message ---- Histonet Digest, Vol 58, Issue 13 Message: 11 Date: Thu, 11 Sep 2008 12:04:13 +0200 From: "Bruijntjes, J.P. (Joost)" Subject: [Histonet] BrdU Hi Histonetters I do have a problem with BrdU, and I hope someone of you can give a kind of solution/explanation. I work with NALT (Nose Associated Lymphoid Tissue) of both rats and mice, which were fixed in a fixative composed of acetic acid, formaldehyde, ethanol and aqua dest. After 48 hours this fixative was replaced by ethanol. Later on the tissues were dehydrated and embedded in paraffin. Just because the lymphoid tissues material is so small, about 10 paraffin slides were collected. I started with the rat NALT's. One paraffin slide was stained with HE, and a few other slides were stained with a monoclonal antibody directed against BrdU. I was satisfied with the staining, so far no problem. After the rats, the same story with the mice NALT's. But I did not get any positive staining. Some days later I repeated the first try-out included with some positive controls from the rat-study with the primary antibody I used in the first part, and the biotin conjugated primary antibody as well, but again no positive staining. The pre-treatment of the slides for rat and mice is the same. The only difference lies in the primary and secondary antibody. For rat tissue I use a non-conjugated monoclonal antibody, followed by HRP-conjugated powervision. For mice tissue I use a biotin conjugated monoclonal antibody, followed by a HRP-conjugated streptavidin. Can anyone give me an explanation? The storage of the slides was in a room with an equal temperature (about 20-21?C) and humidity. Is it possible that the epitopes in the single slides are destroyed so that they are not recognizable anymore by the antibody? The time between preparing the slides and the BrdU staining of the rats NALT slides was at the most 4 weeks, while the first negative staining on the mice NALT's appeared after about 8 weeks. Thanks in advance Joost Bruijntjes TNO Quality of Life Zeist The Netherlands TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl David A. Wright, Ph.D. University of Chicago Section of Neurosurgery, MC3026 5841 S. Maryland Ave Chicago, IL 60637 USA [W] 773-834-1485 (Office) SBRI Rm J328 773-834-0395 (Lab) SBRI Rm J338 [H] 773-643-6976 Pager (@773-753-1880) #9776 Text page 120 char E-mail 7738456834@myairmail.com or web: http://www.myairmail.com to 7738456834 FAX (Neurosurgery) 773.702.3518 Web FAX & personal voicemail: (815)642-9630 Skype: davidanthonywright ================================================ Does 2+2=5 for large values of 2? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From borsay.dodi <@t> epamail.epa.gov Tue Sep 16 13:06:57 2008 From: borsay.dodi <@t> epamail.epa.gov (borsay.dodi@epamail.epa.gov) Date: Mon Sep 22 10:25:08 2008 Subject: [Histonet] Oversized microscope slide source Message-ID: Here's a website for a company based in California, US. http://www.tedpella.com/histo_html/slides-large.htm The UK distributor is: Agar Scientific Ltd 66a Cambridge Road Stansted, Essex CM24 8DA United Kingdom Tel.: 44-1279-813519 sales@agarscientific.com http://www.agarscientific.com Dodi Borsay Horowitz, Biologist US Environmental Protection Agency Atlantic Ecology Division 27 Tarzwell Drive Narragansett, Rhode Island 02882 401/782-3042 borsay.dodi@epamail.epa.gov From radhikab <@t> drreddys.com Fri Sep 19 00:49:08 2008 From: radhikab <@t> drreddys.com (radhikab@drreddys.com) Date: Mon Sep 22 10:25:10 2008 Subject: [Histonet] Microtome micron calibration and validation Message-ID: Hi, I want to know ur comments and thoughts on microtome micron calibration and validation.Is there any method for doing it,please explain Thanks and Regards B.Radhika Junior Scientist Preclinical safety evaluation Dr Reddy's Discovery Research Disclaimer This message contains legally privileged and/or confidential information. If you are not the intended recipient(s), or employee or agent responsible for delivery of this message to the intended recipient(s), you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this message in error, please immediately notify the sender and delete this e-mail message from your computer. WARNING: Computer viruses can be transmitted via email. The recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email. From hambrightd <@t> mail.nih.gov Mon Sep 22 10:44:22 2008 From: hambrightd <@t> mail.nih.gov (Dustin Hambright) Date: Mon Sep 22 10:44:32 2008 Subject: [Histonet] Beta-Gal antibody Message-ID: Hello, Does anyone know a reliable source for a Beta-Gal (lac-z) antibody for mouse frozen immunohistochemistry? Do you know the company and dilution? Thank you in advance, Dustin From JMacDonald <@t> mtsac.edu Mon Sep 22 11:18:43 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Sep 22 11:18:54 2008 Subject: [Histonet] CE opportunity Message-ID: Continuing Education Opportunity (3 CE units) Diagnostic BioSystems is sponsoring a workshop in cooperation with the California Society for Histotechnology. The workshop will be held at Mt. San Antonio College. Date: Saturday, October 18 Time: 9:30 AM to 1:00 PM Title: IHC: Basics and Beyond Maximum attendance: 60 For more information or to RSVP, please contact Jennifer MacDonald at jmacdonald@mtsac.edu From Lynn.Burton <@t> Illinois.gov Mon Sep 22 11:38:32 2008 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Mon Sep 22 11:40:20 2008 Subject: [Histonet] Humidity levels in the lab References: <040346FA7309BD439C327F97D4C4D69B04A1C06C@ISIS.fhcrc.org> <90354A475B420441B2A0396E5008D496731768@copc-sbs.COPC.local> Message-ID: In the summertime ours is at around 60-70%, which seems to work well. In the winter time it is less than 0 and I run a humidifier full out to try to achieve 20%. It is definitely easier in the summer. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Thomas Jasper Sent: Fri 9/19/2008 5:01 PM To: Randolph-Habecker, Julie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Humidity levels in the lab Julie, I can't offer you any references, but here's something to consider. A cool mist humidifier in your lab. We are in Bend, OR., on the dry side of the Cascades. Running the humidifier reduces static and makes sectioning a bit easier. No hard science with this, just a little more atmospheric moisture. By the way, the humidifier I bought is a big blue and white penguin. The mist streams out the beak. Just looking at it makes you smile. I bought it at Target for ~ $40.00 - it works for us. Have a good weekend. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Randolph-Habecker, Julie Sent: Thursday, September 18, 2008 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Humidity levels in the lab Folks, We just moved to a new lab space in a new building. We are currently running at 45% or lower humidity. I am working with our facilities on raising the level but I am looking for some references for what level we should aim for in regard to paraffin cutting. Does anyone have some ideas or places to look for information? Thanks!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tchistology <@t> earthlink.net Mon Sep 22 12:53:19 2008 From: tchistology <@t> earthlink.net (Randall Carpenter) Date: Mon Sep 22 12:53:25 2008 Subject: [Histonet] Re: Microtome micron calibration and validation Message-ID: <6919864.1222106000120.JavaMail.root@elwamui-little.atl.sa.earthlink.net> I just did an IQ, OQ, PQ on my process. The OQ on the microtome involved using a dial micrometer. Set the thickness at 5 microns. Tare or zero the dial micrometer on a point on your sample holder. Pull the micrometer point back, turn the wheel X number of times and take your new reading. It should add up to X times 5 microns +/- your tolerances. The rest of the "validation" process depends on it being installed properly and that it actually cuts sections suitable for pathologic interpretation. As we all know, just because the dial on the microtome says 5, doesn't mean the section is 5 microns. The point of the Operation Qualification is only to demonstrate that the machine works like it's supposed to. The dial micrometer needs to be calibrated/traceable. I got a guy in town to do it for $20. Of course everything needs to be documented and QA should sign off on it. Hope it helps. Randy Carpenter Twin Cities Histology Hi, I want to know ur comments and thoughts on microtome micron calibration and validation.Is there any method for doing it,please explain Thanks and Regards B.Radhika Junior Scientist Preclinical safety evaluation Dr Reddy's Discovery Research From RSRICHMOND <@t> aol.com Mon Sep 22 12:53:29 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Mon Sep 22 12:53:34 2008 Subject: [Histonet] Re: Humidity levels in the lab Message-ID: >>In the summertime [the humidity in our laboratory] is at around 60-70%, which seems to work well. In the winter time it is less than 0 and I run a humidifier full out to try to achieve 20%.<< If your relative humidity is less than zero, you're probably putting too much thiotimoline in your water bath! (If you're too young for this literary allusion, see http://en.wikipedia.org/wiki/Thiotimoline ) and make the acquaintance of the late great Isaac Asimov! Bob Richmond Samurai Pathologist Knoxville TN From Jerry <@t> ralambusa.com Mon Sep 22 13:04:56 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Mon Sep 22 13:05:07 2008 Subject: [Histonet] RE: Oversized microscope supply source (IN USA) Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B5CA8@server.ralambusa.com> We also supply many larger sizes, see www.ralamb.com Thanks, ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, September 22, 2008 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 58, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Oversized microscope slide source (borsay.dodi@epamail.epa.gov) 2. Microtome micron calibration and validation (radhikab@drreddys.com) 3. Beta-Gal antibody (Dustin Hambright) 4. CE opportunity (Jennifer MacDonald) 5. RE: Humidity levels in the lab (Burton, Lynn) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Sep 2008 14:06:57 -0400 From: borsay.dodi@epamail.epa.gov Subject: [Histonet] Oversized microscope slide source To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Here's a website for a company based in California, US. http://www.tedpella.com/histo_html/slides-large.htm The UK distributor is: Agar Scientific Ltd 66a Cambridge Road Stansted, Essex CM24 8DA United Kingdom Tel.: 44-1279-813519 sales@agarscientific.com http://www.agarscientific.com Dodi Borsay Horowitz, Biologist US Environmental Protection Agency Atlantic Ecology Division 27 Tarzwell Drive Narragansett, Rhode Island 02882 401/782-3042 borsay.dodi@epamail.epa.gov ------------------------------ Message: 2 Date: Fri, 19 Sep 2008 11:19:08 +0530 From: radhikab@drreddys.com Subject: [Histonet] Microtome micron calibration and validation To: Histonet@lists.utsouthwestern.edu, clarkda@ohsu.edu Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, I want to know ur comments and thoughts on microtome micron calibration and validation.Is there any method for doing it,please explain Thanks and Regards B.Radhika Junior Scientist Preclinical safety evaluation Dr Reddy's Discovery Research Disclaimer This message contains legally privileged and/or confidential information. If you are not the intended recipient(s), or employee or agent responsible for delivery of this message to the intended recipient(s), you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this message in error, please immediately notify the sender and delete this e-mail message from your computer. WARNING: Computer viruses can be transmitted via email. The recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email. ------------------------------ Message: 3 Date: Mon, 22 Sep 2008 11:44:22 -0400 From: Dustin Hambright Subject: [Histonet] Beta-Gal antibody To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello, Does anyone know a reliable source for a Beta-Gal (lac-z) antibody for mouse frozen immunohistochemistry? Do you know the company and dilution? Thank you in advance, Dustin ------------------------------ Message: 4 Date: Mon, 22 Sep 2008 09:18:43 -0700 From: Jennifer MacDonald Subject: [Histonet] CE opportunity To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Continuing Education Opportunity (3 CE units) Diagnostic BioSystems is sponsoring a workshop in cooperation with the California Society for Histotechnology. The workshop will be held at Mt. San Antonio College. Date: Saturday, October 18 Time: 9:30 AM to 1:00 PM Title: IHC: Basics and Beyond Maximum attendance: 60 For more information or to RSVP, please contact Jennifer MacDonald at jmacdonald@mtsac.edu ------------------------------ Message: 5 Date: Mon, 22 Sep 2008 11:38:32 -0500 From: "Burton, Lynn" Subject: RE: [Histonet] Humidity levels in the lab To: "Thomas Jasper" , "Randolph-Habecker, Julie" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" In the summertime ours is at around 60-70%, which seems to work well. In the winter time it is less than 0 and I run a humidifier full out to try to achieve 20%. It is definitely easier in the summer. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Thomas Jasper Sent: Fri 9/19/2008 5:01 PM To: Randolph-Habecker, Julie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Humidity levels in the lab Julie, I can't offer you any references, but here's something to consider. A cool mist humidifier in your lab. We are in Bend, OR., on the dry side of the Cascades. Running the humidifier reduces static and makes sectioning a bit easier. No hard science with this, just a little more atmospheric moisture. By the way, the humidifier I bought is a big blue and white penguin. The mist streams out the beak. Just looking at it makes you smile. I bought it at Target for ~ $40.00 - it works for us. Have a good weekend. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Randolph-Habecker, Julie Sent: Thursday, September 18, 2008 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Humidity levels in the lab Folks, We just moved to a new lab space in a new building. We are currently running at 45% or lower humidity. I am working with our facilities on raising the level but I am looking for some references for what level we should aim for in regard to paraffin cutting. Does anyone have some ideas or places to look for information? Thanks!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 58, Issue 26 **************************************** From Jackie.O'Connor <@t> abbott.com Mon Sep 22 14:54:20 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Sep 22 14:54:46 2008 Subject: [Histonet] Histology schools/training in San Antonio, TX In-Reply-To: <982A0A9461F9BF438C7B19A6E425A383637681@ITSSSXM01V6.one.ads.che.org> Message-ID: Can someone advise me of the schools available in the San Antonio area, and what the prerequisites are? Thanks, Jackie From histobeach <@t> aol.com Mon Sep 22 15:28:07 2008 From: histobeach <@t> aol.com (histobeach@aol.com) Date: Mon Sep 22 15:28:20 2008 Subject: [Histonet] Microtome Message-ID: <8CAEB08DB6DE055-EF4-16E5@webmail-nc12.sysops.aol.com> Hello, We will be purchasing another microtome and I wanted to get feedback from the list.? If you wouldn't mind giving me your thoughts on the microtome you like the best.? We are not getting an automated one, not in our budget, but a simple, ever-lasting one.? We have the Leica 2135 and 2125 and sometimes the blade holder gives us problems, but I'm sure they all do.? Actually, we do have a Microm 330 and that one hasn't given us any problems at all. I just would like to know the one that gets a lot of positive feedback. Thanks so much in advance, Stacey, HT Biomed?Group From michael.owen <@t> fda.hhs.gov Mon Sep 22 15:30:48 2008 From: michael.owen <@t> fda.hhs.gov (Owen, Michael P) Date: Mon Sep 22 15:30:52 2008 Subject: [Histonet] Seattle-Tacoma craigslist: Manager/Senior Manager, Histology and Laboratory Resources (Bothell, WA) Message-ID: <449E51C6DA0AD840B44F57C7A6EB07BF04220657@FMD3VS022.fda.gov> seattle-tacoma craigslist / jobs / biotech/sci http://seattle.craigslist.org http://seattle.craigslist.org/see/sci/850615554.html Manager/Senior Manager, Histology and Laboratory Resources (Bothell, WA) ------------------------------------------------------------------------ -------- Reply to: see below Date: 2008-09-22, 9:23AM PDT Would you like to work for a premier provider of innovative solutions in drug discovery and development? MDS Pharma Services has an exciting opportunity as a Manager/Senior Manager, Histology and Laboratory Resources at our Bothell, WA location. In this full-time position, you will be responsible for the management of our histology and bioassay laboratory and staff in support of preclinical efficacy pharmacology studies. The job is expected to be split approximately 70% managerial functions and 30% histology or bioassay laboratory bench work. Technical experience in the preparation of paraffin, plastic and/or frozen tissue specimens is required. As the laboratory manager, you will develop and administer budgets, performance requirements, and schedules. You will also monitor and control labor and capital expenditures and will interpret results of laboratory activities. You will provide operational oversight of the laboratory and technical guidance with regard to feasibility and timing to project teams. Other staff functions include interviewing, hiring, and training employees as well as addressing staff complaints and oversight of the development of new methods or assays. A BS/BA degree in a scientific field and 8+ years of experience is desired along with 2+ years of supervisory/management experience. Equivalent education and experience is also acceptable. Experience in histology and /or, histomorphometry is required. Experience with ELISA, RIA or clinical pathology laboratory procedures are highly desirable. Experience in working skeletal or CNS diseases is a plus. Familiarity with GLP and CLIA requirements are desirable as well. AA/EEO Please apply on-line at www.mdsps.com. Location: Bothell, WA Compensation: Great pay and benefits! Principals only. Recruiters, please don't contact this job poster. Please, no phone calls about this job! Please do not contact job poster about other services, products or commercial interests. PostingID: 850615554 Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From saby_joseph_a <@t> yahoo.com Mon Sep 22 19:00:37 2008 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Mon Sep 22 19:00:42 2008 Subject: [Histonet] Cassette Holders Message-ID: <857916.89293.qm@web33804.mail.mud.yahoo.com> Jennifer- ? Be of good cheer! ? I saw them at the NSH convention. ? Now if I could only remember where... ? Joe ----- Original Message ---- From: "histoinfo@comcast.net" To: Histonet@lists.utsouthwestern.edu Sent: Thursday, September 18, 2008 3:47:34 PM Subject: [Histonet] Cassette Holders Greetings Netters, For those of you that remember the old metal cassette holders. It seems that they are no longer available (no surprise), and I cant find anything like them on the market. They were sold by tissue-tek I think. They are about 4 inches square and one inch tall. They have 5 rows that hold 3 cassettes each for a total of 15 cassettes. We are looking for more of these, if anyone has some they are no longer using please contact me. Many Thanks, Jennifer Sauners HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ht.ascp <@t> yahoo.com Mon Sep 22 19:11:33 2008 From: ht.ascp <@t> yahoo.com (Jon Google) Date: Mon Sep 22 19:11:37 2008 Subject: [Histonet] BioCare Message-ID: <681179.96425.qm@web59916.mail.ac4.yahoo.com> Did anyone see the new biocare IHC stainer at NSH? could you give your impressions?? We are looking at the Bond, Leica Bond, but we have just heard about the biocare system. ? Thanks in advance, From godsgalnow <@t> aol.com Mon Sep 22 19:33:18 2008 From: godsgalnow <@t> aol.com (Roxanne Soto) Date: Mon Sep 22 19:34:36 2008 Subject: [Histonet] BioCare In-Reply-To: <681179.96425.qm@web59916.mail.ac4.yahoo.com> References: <681179.96425.qm@web59916.mail.ac4.yahoo.com> Message-ID: <9E679682-16C0-43F1-B524-588DD966F603@aol.com> This happens to be my favorite of all the newer instruments. If it does everything proposed-wow, what a time saver it would be. To be able to load it without the other program being finished. DAKO tried an instrument like this a couple of years ago but it bombed. I would set yourself up for a demo. Once they are released they are going to go quick- we are getting 4 of them throughout our system. Sent from my iPhone On Sep 22, 2008, at 8:11 PM, Jon Google wrote: > Did anyone see the new biocare IHC stainer at NSH? could you give > your impressions? We are looking at the Bond, Leica Bond, but we > have just heard about the biocare system. > > Thanks in advance, > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Christian.Orlowski <@t> petermac.org Mon Sep 22 23:02:15 2008 From: Christian.Orlowski <@t> petermac.org (Orlowski Christian) Date: Mon Sep 22 23:06:59 2008 Subject: [Histonet] Cryosectioning dorsal mouse skin Message-ID: Greetings all, I'm wondering whether there are any recommendations for how to effectively cryosection dorsal mouse skin for immunohistochemistry. I'm having difficulty with much of the mouse skin slipping off the slide during the fixation and washing steps, which seems to result in loss of the epidermis in particular, that part of the tissue that I happen to be interested in. I apply the dorsal mouse skin cryosections onto SuperFrost Plus (Menzel-Glaser) slides and air dry them for 2 hours. I use acetone for fixation, 10 minutes, and use PBS for washing. Any suggestions? This email (including any attachments or links) may contain confidential and/or legally privileged information and is intended only to be read or used by the addressee. If you are not the intended addressee, any use, distribution, disclosure or copying of this email is strictly prohibited. Confidentiality and legal privilege attached to this email (including any attachments) are not waived or lost by reason of its mistaken delivery to you. If you have received this email in error, please delete it and notify us immediately by telephone or email. Peter MacCallum Cancer Centre provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered and will not be liable for any delay in its receipt. From sohail_e <@t> yahoo.com Tue Sep 23 05:37:21 2008 From: sohail_e <@t> yahoo.com (Sohail Ejaz) Date: Tue Sep 23 05:37:25 2008 Subject: [Histonet] detection of brain infarcts Message-ID: <551193.17390.qm@web39503.mail.mud.yahoo.com> HI Everybody ? I am trying to find some stain that can detect brain infarct. Plreviously i was using TTC, but now we have ban on that. ? So, looking forward for a best alternative. ? Thanks ? Sohail From mprice26 <@t> juno.com Tue Sep 23 07:07:08 2008 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Tue Sep 23 07:09:00 2008 Subject: [Histonet] Re: Blind Review Policy Message-ID: <20080923.070708.18021.0@webmail06.dca.untd.com> Does anyone have a Blind Case review Policy they could share with me? This is where when there is only one Pathologist and he does not have another pathologist to do peer review,In a one Pathologist lab. Is anyone else doing this? Thank you. Marsha Price ____________________________________________________________ Click to consolidate your debt in minutes, stop late or over-limit fees, pay less. http://thirdpartyoffers.juno.com/TGL2141/fc/Ioyw6i3m2bkZgujH4vUMxAF4GxmsePRHuCIW6Y6ywMqxb9PWv5ADBn/ From froyer <@t> bitstream.net Tue Sep 23 08:37:15 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Tue Sep 23 08:37:30 2008 Subject: [Histonet] Cassette Holders In-Reply-To: <857916.89293.qm@web33804.mail.mud.yahoo.com> References: <857916.89293.qm@web33804.mail.mud.yahoo.com> Message-ID: I am not sure if this is what you are looking for but it is the one that I am familiar with that is still being manufactured. http://www.innovativelabacrylics.com/products/pages/cassette.html Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 Web: http://www.minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joseph Saby Sent: Monday, September 22, 2008 7:01 PM To: histoinfo@comcast.net; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cassette Holders Jennifer- ? Be of good cheer! ? I saw them at the NSH convention. ? Now if I could only remember where... ? Joe ----- Original Message ---- From: "histoinfo@comcast.net" To: Histonet@lists.utsouthwestern.edu Sent: Thursday, September 18, 2008 3:47:34 PM Subject: [Histonet] Cassette Holders Greetings Netters, For those of you that remember the old metal cassette holders. It seems that they are no longer available (no surprise), and I cant find anything like them on the market. They were sold by tissue-tek I think. They are about 4 inches square and one inch tall. They have 5 rows that hold 3 cassettes each for a total of 15 cassettes. We are looking for more of these, if anyone has some they are no longer using please contact me. Many Thanks, Jennifer Sauners HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dusko.trajkovic <@t> pfizer.com Tue Sep 23 08:58:05 2008 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Tue Sep 23 08:58:11 2008 Subject: [Histonet] Effect of 5%Aeti acid on Nuclei In-Reply-To: Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B2080F9C1C@lajamrexm01.amer.pfizer.com> Good day to Everyone and for those that attended the NSH, WELCOME BACK. I would hope that the parties were good and I am truly sorry that I had to miss all of them. I have a friend who asked me to post this for him. Any answer or suggestion would be appreciated. Thank you Dusko Trajkovic I'm asking because in a paper I'm reviewing a paper where someone is using a concentration of 45-60% Acetic acid to fix isolated nuclei, then fixing again with Carnoy's, and then doing FISH (fluorescence in situ hyb) on them. I'm trying to figure out the effects on chromatin...does it cause the nuclei and/or chromatin to swell? I'd appreciate if you could ask around. Thanks, Yun From rjbuesa <@t> yahoo.com Tue Sep 23 09:11:22 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 23 09:11:28 2008 Subject: [Histonet] Effect of 5%Aeti acid on Nuclei In-Reply-To: <3AD0BD3142459B4E9B12CBEAFF2B89B2080F9C1C@lajamrexm01.amer.pfizer.com> Message-ID: <509501.27454.qm@web65703.mail.ac4.yahoo.com> Yes, the immediate effect of acetic is nuclear swelling. Ren?? J. --- On Tue, 9/23/08, Trajkovic, Dusko wrote: From: Trajkovic, Dusko Subject: [Histonet] Effect of 5%Aeti acid on Nuclei To: Histonet@lists.utsouthwestern.edu Date: Tuesday, September 23, 2008, 9:58 AM Good day to Everyone and for those that attended the NSH, WELCOME BACK. I would hope that the parties were good and I am truly sorry that I had to miss all of them. I have a friend who asked me to post this for him. Any answer or suggestion would be appreciated. Thank you Dusko Trajkovic I'm asking because in a paper I'm reviewing a paper where someone is using a concentration of 45-60% Acetic acid to fix isolated nuclei, then fixing again with Carnoy's, and then doing FISH (fluorescence in situ hyb) on them. I'm trying to figure out the effects on chromatin...does it cause the nuclei and/or chromatin to swell? I'd appreciate if you could ask around. Thanks, Yun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mprice26 <@t> juno.com Tue Sep 23 09:47:51 2008 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Tue Sep 23 09:59:57 2008 Subject: [Histonet] Re: Blind Review Policy Message-ID: <20080923.094751.6345.0@webmail10.dca.untd.com> Does anyone have a policy for a Blind Case Review for a one pathologist private lab? Thank you. Marsha Price ____________________________________________________________ Internet Security Software - Click here. http://thirdpartyoffers.juno.com/TGL2141/fc/Ioyw6i3mEWrnsLu04AAPUy8TIFwZG9ifrjPeX3laAPLUa845TgE3Sp/ From HACKERLAB <@t> aol.com Tue Sep 23 10:53:17 2008 From: HACKERLAB <@t> aol.com (HACKERLAB@aol.com) Date: Tue Sep 23 10:53:43 2008 Subject: [Histonet] Cassette Holders Message-ID: Hello Jennifer; In response to your search for cassette holders, please check out this link: _http://www.hackerinstruments.com/labstorage.htm_ (http://www.hackerinstruments.com/labstorage.htm) They were on display at the NSH meeting in Pittsburgh. Best regards, Elfi Hacker In a message dated 9/18/2008 3:48:03 P.M. Eastern Daylight Time, histoinfo@comcast.net writes: Greetings Netters, For those of you that remember the old metal cassette holders. It seems that they are no longer available (no surprise), and I cant find anything like them on the market. They were sold by tissue-tek I think. They are about 4 inches square and one inch tall. They have 5 rows that hold 3 cassettes each for a total of 15 cassettes. We are looking for more of these, if anyone has some they are no longer using please contact me. Many Thanks, Jennifer Sauners HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************Looking for simple solutions to your real-life financial challenges? Check out WalletPop for the latest news and information, tips and calculators. (http://www.walletpop.com/?NCID=emlcntuswall00000001) From jcline <@t> wchsys.org Tue Sep 23 11:48:14 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue Sep 23 11:49:16 2008 Subject: [Histonet] Ventana Retic Stain In-Reply-To: <57BE698966D5C54EAE8612E8941D768303BD4484@EXCHANGE3.huntingtonhospital.com> Message-ID: We have to let our retic slides dry for 1 hour at 72C. We use superfrost plus slides otherwise we have variable staining or no staining. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 18, 2008 3:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Retic Stain Is anyone out there having problems with their Retic stain on the Ventana Nexus Special Stainer? Please contact me offline. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From aevans <@t> wellspan.org Tue Sep 23 12:30:19 2008 From: aevans <@t> wellspan.org (Evans, Andria B.) Date: Tue Sep 23 12:40:39 2008 Subject: [Histonet] Dako Party Message-ID: I was wondering if anyone has any pictures of the Dako Party from the NSH Convention. I dropped my camera the night before and broke it and was not able to take pictures. I would be willing to send a CD for the pictures to be writtin to along with postage to send it back to me. Please email me if you can help. Thanks! Andria B Evans, HTL(ASCP)CM CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From aevans <@t> wellspan.org Tue Sep 23 12:28:13 2008 From: aevans <@t> wellspan.org (Evans, Andria B.) Date: Tue Sep 23 12:40:41 2008 Subject: [Histonet] NSH IHC Qualification Exam Class Message-ID: I was wondering if anyone could send me the handouts from the IHC Qualification class that was presented at the NSH Convention? I wanted to take that class, but ended up taking others. Please email me if you have them. Thanks! Andria B Evans, HTL(ASCP)CM CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From jqb7 <@t> cdc.gov Tue Sep 23 12:42:27 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Sep 23 12:43:20 2008 Subject: [Histonet] Dako Party In-Reply-To: References: Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C213@LTA3VS011.ees.hhs.gov> I think all of the pictures have been confiscated......to protect the not-so-innocent. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B. Sent: Tuesday, September 23, 2008 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Party I was wondering if anyone has any pictures of the Dako Party from the NSH Convention. I dropped my camera the night before and broke it and was not able to take pictures. I would be willing to send a CD for the pictures to be writtin to along with postage to send it back to me. Please email me if you can help. Thanks! Andria B Evans, HTL(ASCP)CM CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Tue Sep 23 12:43:58 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Sep 23 12:44:02 2008 Subject: [Histonet] texas spring meeting In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82E28@EMAIL.archildrens.org> If anyone from Texas can give me any details of your spring meeting '09, I would appreciate it. Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From sjchtascp <@t> yahoo.com Tue Sep 23 12:47:41 2008 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Tue Sep 23 12:47:48 2008 Subject: [Histonet] Histology Text Message-ID: <137300.4313.qm@web38206.mail.mud.yahoo.com> I'm looking for a good overall used histology text.? Maybe Carsons, Luna, or AFIP ect. ? Steve From JGarfield <@t> lifecell.com Tue Sep 23 12:50:31 2008 From: JGarfield <@t> lifecell.com (Garfield, Jacqueline) Date: Tue Sep 23 12:50:35 2008 Subject: [Histonet] RE: NSH IHC Qualification Exam Class In-Reply-To: Message-ID: <81418DA27DB07A4FB89264117C6EA4989FBD008DAB@LC-HQEXCH01.lifecell.biz> I was in the same boat. If anyone is willing to share, I greatly appreciate it. Sincerely, Jackie Garfield Jacqueline D. Garfield | Manager, Histology Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1182 Email jgarfield@ lifecell.com www.lifcell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B. Sent: Tuesday, September 23, 2008 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH IHC Qualification Exam Class I was wondering if anyone could send me the handouts from the IHC Qualification class that was presented at the NSH Convention? I wanted to take that class, but ended up taking others. Please email me if you have them. Thanks! Andria B Evans, HTL(ASCP)CM CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *********************************************************************************************************************************************** This e-mail message and any attachments are confidential. Dissemination, distribution or copying of this e-mail or any attachments by anyone other than the intended recipient is prohibited. If you are not the intended recipient, please notify LifeCell Corporation immediately by replying to this e-mail, and destroy all copies of this e-mail and any attachments. Thank you! *********************************************************************************************************************************************** From GDawson <@t> dynacaremilwaukee.com Tue Sep 23 12:54:00 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Sep 23 12:54:07 2008 Subject: [Histonet] Dermatology bx. embedding guide In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A38C4@IS-E2K3.grhs.net> Message-ID: All, I am searching for an illustrated guide to embedding various derm biopsies. If anyone could forward one to me (or point me in the right direction to obtain one), I would be eternally grateful. The ideal guide would be one with pictures that could be posted next to the embedding station. Thanx in Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From lblazek <@t> digestivespecialists.com Tue Sep 23 13:05:44 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Sep 23 13:05:33 2008 Subject: [Histonet] Dako Party In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A70208C213@LTA3VS011.ees.hhs.gov> References: <1CE1847DFEA0A647B1CCDE4108EA60A70208C213@LTA3VS011.ees.hhs.gov> Message-ID: <5A2BD13465E061429D6455C8D6B40E39058C042DE2@IBMB7Exchange.digestivespecialists.com> I hope so. Hummmm I did end up with a CD though. I wonder what's on it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, September 23, 2008 1:42 PM To: Evans, Andria B.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako Party I think all of the pictures have been confiscated......to protect the not-so-innocent. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B. Sent: Tuesday, September 23, 2008 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Party I was wondering if anyone has any pictures of the Dako Party from the NSH Convention. I dropped my camera the night before and broke it and was not able to take pictures. I would be willing to send a CD for the pictures to be writtin to along with postage to send it back to me. Please email me if you can help. Thanks! Andria B Evans, HTL(ASCP)CM CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pumla.pamla <@t> gmail.com Tue Sep 23 13:53:59 2008 From: pumla.pamla <@t> gmail.com (Pumla Pamla-Gutter) Date: Tue Sep 23 13:54:03 2008 Subject: [Histonet] Glycerol jelly Message-ID: I've recently started using home-made glycerol jelly as my mounting medium---composition gelatin powder, water, and glycerol. I keep it in the fridge at 4 degrees and melt it before use. It's great for dark field images, but I'm worried about bacterial growth. I haven't had any problems yet, but I was wondering if someone else out there uses this and what they do to prevent the growth of mold, etc... and how long do you keep it before you toss it? Thanks, Pumla Pamla-Gutter (614)292-4046 From llewllew <@t> shaw.ca Tue Sep 23 14:04:52 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Sep 23 14:04:54 2008 Subject: [Histonet] Histology Text References: <137300.4313.qm@web38206.mail.mud.yahoo.com> Message-ID: <5198E6143A5D47F0ADCCCE453A3751FB@Compaq> Go to http://www.abebooks.com/ and do a search on the author's name or "histology", "histological", "staining" etc. They usually list quite a lot of them. Bryan Llewellyn ----- Original Message ----- From: "Steven Coakley" To: Sent: Tuesday, September 23, 2008 10:47 AM Subject: [Histonet] Histology Text I'm looking for a good overall used histology text. Maybe Carsons, Luna, or AFIP ect. Steve _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Tue Sep 23 14:06:52 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Sep 23 14:06:53 2008 Subject: [Histonet] Glycerol jelly References: Message-ID: <7F92A8E1A79C40209315A10BF0B3A242@Compaq> It was usually recommended to add a crystal of thymol, that should preserve it for months. Bryan Llewellyn ----- Original Message ----- From: "Pumla Pamla-Gutter" To: Sent: Tuesday, September 23, 2008 11:53 AM Subject: [Histonet] Glycerol jelly > I've recently started using home-made glycerol jelly as my mounting > medium---composition gelatin powder, water, and glycerol. I keep it in > the fridge at 4 degrees and melt it before use. It's great for dark > field images, but I'm worried about bacterial growth. I haven't had > any problems yet, but I was wondering if someone else out there uses > this and what they do to prevent the growth of mold, etc... and how > long do you keep it before you toss it? > Thanks, > Pumla Pamla-Gutter > (614)292-4046 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Tue Sep 23 14:09:36 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Sep 23 14:09:41 2008 Subject: [Histonet] Re Blind Review Policy Message-ID: Marsha Price asks: >>Does anyone have a Blind Case review Policy they could share with me? This is where when there is only one Pathologist and he does not have another pathologist to do peer review,In a one Pathologist lab. Is anyone else doing this?<< In my travels as a locum tenens pathologist, I have rarely seen this done in the many solo pathology practices I have covered. It sounds good to bureaucrats, but in the opinion of most pathologists "blind review" is of very little value. It's much more important to establish a pattern of sending problem cases out for consultation, and maintaining adequate records of these consultations. Bob Richmond Samurai Pathologist Knoxville TN From jfish <@t> gladstone.ucsf.edu Tue Sep 23 14:11:02 2008 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Tue Sep 23 14:11:31 2008 Subject: [Histonet] Glycerol jelly In-Reply-To: References: Message-ID: <000801c91db0$1ecb0140$4e0d010a@JFISH> Hi Pumla, Our recipe includes 1ml or liquified Phenol per 150ml of Jelly. Good luck, Jo Dee ~~Jo Dee Fish~~ Senior Research Technologist The J. David Gladstone Institutes Co-manager Histology and Microscopy Core Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pumla Pamla-Gutter Sent: Tuesday, September 23, 2008 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Glycerol jelly I've recently started using home-made glycerol jelly as my mounting medium---composition gelatin powder, water, and glycerol. I keep it in the fridge at 4 degrees and melt it before use. It's great for dark field images, but I'm worried about bacterial growth. I haven't had any problems yet, but I was wondering if someone else out there uses this and what they do to prevent the growth of mold, etc... and how long do you keep it before you toss it? Thanks, Pumla Pamla-Gutter (614)292-4046 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GoodwinD <@t> pahosp.com Tue Sep 23 14:19:00 2008 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Tue Sep 23 14:20:22 2008 Subject: [Histonet] RE: Biocare IHC instrument In-Reply-To: References: Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E0AF0BC13@uphsmbx2.UPHS.PENNHEALTH.PRV> I was very impressed with the Biocare instrument. It is called the Intellipath. Open system (my favorite feature), continuous feed up to 10 slides per load, able to run 5 protocols simultaneously for a total of 50 slides running simultaneously- can't remember how many protocols it can store. Definitely worth a look. Diana Goodwin Anatomic Pathology Pennsylvania Hospital Philadelphia, PA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, September 23, 2008 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 58, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Microtome micron calibration and validation (Randall Carpenter) 2. Re: Humidity levels in the lab (Robert Richmond) 3. RE: Oversized microscope supply source (IN USA) (Jerry Helisek) 4. Histology schools/training in San Antonio, TX (Jackie M O'Connor) 5. Microtome (histobeach@aol.com) 6. Seattle-Tacoma craigslist: Manager/Senior Manager, Histology and Laboratory Resources (Bothell, WA) (Owen, Michael P) 7. Re: Cassette Holders (Joseph Saby) 8. BioCare (Jon Google) 9. Re: BioCare (Roxanne Soto) 10. Cryosectioning dorsal mouse skin (Orlowski Christian) 11. detection of brain infarcts (Sohail Ejaz) 12. Re: Blind Review Policy (mprice26@juno.com) 13. RE: Cassette Holders (Ford Royer) 14. Effect of 5%Aeti acid on Nuclei (Trajkovic, Dusko) 15. Re: Effect of 5%Aeti acid on Nuclei (Rene J Buesa) 16. Re: Re: Blind Review Policy (mprice26@juno.com) 17. Re: Cassette Holders (HACKERLAB@aol.com) 18. RE: Ventana Retic Stain (Joyce Cline) ---------------------------------------------------------------------- Message: 1 Date: Mon, 22 Sep 2008 10:53:19 -0700 (GMT-07:00) From: Randall Carpenter Subject: [Histonet] Re: Microtome micron calibration and validation To: histonet@lists.utsouthwestern.edu Message-ID: <6919864.1222106000120.JavaMail.root@elwamui-little.atl.sa.earthlink.net> Content-Type: text/plain; charset=UTF-8 I just did an IQ, OQ, PQ on my process. The OQ on the microtome involved using a dial micrometer. Set the thickness at 5 microns. Tare or zero the dial micrometer on a point on your sample holder. Pull the micrometer point back, turn the wheel X number of times and take your new reading. It should add up to X times 5 microns +/- your tolerances. The rest of the "validation" process depends on it being installed properly and that it actually cuts sections suitable for pathologic interpretation. As we all know, just because the dial on the microtome says 5, doesn't mean the section is 5 microns. The point of the Operation Qualification is only to demonstrate that the machine works like it's supposed to. The dial micrometer needs to be calibrated/traceable. I got a guy in town to do it for $20. Of course everything needs to be documented and QA should sign off on it. Hope it helps. Randy Carpenter Twin Cities Histology Hi, I want to know ur comments and thoughts on microtome micron calibration and validation.Is there any method for doing it,please explain Thanks and Regards B.Radhika Junior Scientist Preclinical safety evaluation Dr Reddy's Discovery Research ------------------------------ Message: 2 Date: Mon, 22 Sep 2008 13:53:29 -0400 From: "Robert Richmond" Subject: [Histonet] Re: Humidity levels in the lab To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 >>In the summertime [the humidity in our laboratory] is at around >>60-70%, which seems to work well. In the winter time it is less than 0 >>and I run a humidifier full out to try to achieve 20%.<< If your relative humidity is less than zero, you're probably putting too much thiotimoline in your water bath! (If you're too young for this literary allusion, see http://en.wikipedia.org/wiki/Thiotimoline ) and make the acquaintance of the late great Isaac Asimov! Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 3 Date: Mon, 22 Sep 2008 14:04:56 -0400 From: "Jerry Helisek" Subject: [Histonet] RE: Oversized microscope supply source (IN USA) To: Cc: borsay.dodi@epamail.epa.gov Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B5CA8@server.ralambusa.com> Content-Type: text/plain; charset="us-ascii" We also supply many larger sizes, see www.ralamb.com Thanks, ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, September 22, 2008 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 58, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Oversized microscope slide source (borsay.dodi@epamail.epa.gov) 2. Microtome micron calibration and validation (radhikab@drreddys.com) 3. Beta-Gal antibody (Dustin Hambright) 4. CE opportunity (Jennifer MacDonald) 5. RE: Humidity levels in the lab (Burton, Lynn) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Sep 2008 14:06:57 -0400 From: borsay.dodi@epamail.epa.gov Subject: [Histonet] Oversized microscope slide source To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Here's a website for a company based in California, US. http://www.tedpella.com/histo_html/slides-large.htm The UK distributor is: Agar Scientific Ltd 66a Cambridge Road Stansted, Essex CM24 8DA United Kingdom Tel.: 44-1279-813519 sales@agarscientific.com http://www.agarscientific.com Dodi Borsay Horowitz, Biologist US Environmental Protection Agency Atlantic Ecology Division 27 Tarzwell Drive Narragansett, Rhode Island 02882 401/782-3042 borsay.dodi@epamail.epa.gov ------------------------------ Message: 2 Date: Fri, 19 Sep 2008 11:19:08 +0530 From: radhikab@drreddys.com Subject: [Histonet] Microtome micron calibration and validation To: Histonet@lists.utsouthwestern.edu, clarkda@ohsu.edu Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, I want to know ur comments and thoughts on microtome micron calibration and validation.Is there any method for doing it,please explain Thanks and Regards B.Radhika Junior Scientist Preclinical safety evaluation Dr Reddy's Discovery Research Disclaimer This message contains legally privileged and/or confidential information. If you are not the intended recipient(s), or employee or agent responsible for delivery of this message to the intended recipient(s), you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this message in error, please immediately notify the sender and delete this e-mail message from your computer. WARNING: Computer viruses can be transmitted via email. The recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email. ------------------------------ Message: 3 Date: Mon, 22 Sep 2008 11:44:22 -0400 From: Dustin Hambright Subject: [Histonet] Beta-Gal antibody To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello, Does anyone know a reliable source for a Beta-Gal (lac-z) antibody for mouse frozen immunohistochemistry? Do you know the company and dilution? Thank you in advance, Dustin ------------------------------ Message: 4 Date: Mon, 22 Sep 2008 09:18:43 -0700 From: Jennifer MacDonald Subject: [Histonet] CE opportunity To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Continuing Education Opportunity (3 CE units) Diagnostic BioSystems is sponsoring a workshop in cooperation with the California Society for Histotechnology. The workshop will be held at Mt. San Antonio College. Date: Saturday, October 18 Time: 9:30 AM to 1:00 PM Title: IHC: Basics and Beyond Maximum attendance: 60 For more information or to RSVP, please contact Jennifer MacDonald at jmacdonald@mtsac.edu ------------------------------ Message: 5 Date: Mon, 22 Sep 2008 11:38:32 -0500 From: "Burton, Lynn" Subject: RE: [Histonet] Humidity levels in the lab To: "Thomas Jasper" , "Randolph-Habecker, Julie" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" In the summertime ours is at around 60-70%, which seems to work well. In the winter time it is less than 0 and I run a humidifier full out to try to achieve 20%. It is definitely easier in the summer. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Thomas Jasper Sent: Fri 9/19/2008 5:01 PM To: Randolph-Habecker, Julie Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Humidity levels in the lab Julie, I can't offer you any references, but here's something to consider. A cool mist humidifier in your lab. We are in Bend, OR., on the dry side of the Cascades. Running the humidifier reduces static and makes sectioning a bit easier. No hard science with this, just a little more atmospheric moisture. By the way, the humidifier I bought is a big blue and white penguin. The mist streams out the beak. Just looking at it makes you smile. I bought it at Target for ~ $40.00 - it works for us. Have a good weekend. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Randolph-Habecker, Julie Sent: Thursday, September 18, 2008 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Humidity levels in the lab Folks, We just moved to a new lab space in a new building. We are currently running at 45% or lower humidity. I am working with our facilities on raising the level but I am looking for some references for what level we should aim for in regard to paraffin cutting. Does anyone have some ideas or places to look for information? Thanks!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. DE-360 (Please note new location) Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 58, Issue 26 **************************************** ------------------------------ Message: 4 Date: Mon, 22 Sep 2008 14:54:20 -0500 From: Jackie M O'Connor Subject: [Histonet] Histology schools/training in San Antonio, TX To: "histonet" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Can someone advise me of the schools available in the San Antonio area, and what the prerequisites are? Thanks, Jackie ------------------------------ Message: 5 Date: Mon, 22 Sep 2008 16:28:07 -0400 From: histobeach@aol.com Subject: [Histonet] Microtome To: histonet@lists.utsouthwestern.edu Message-ID: <8CAEB08DB6DE055-EF4-16E5@webmail-nc12.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hello, We will be purchasing another microtome and I wanted to get feedback from the list.? If you wouldn't mind giving me your thoughts on the microtome you like the best.? We are not getting an automated one, not in our budget, but a simple, ever-lasting one.? We have the Leica 2135 and 2125 and sometimes the blade holder gives us problems, but I'm sure they all do.? Actually, we do have a Microm 330 and that one hasn't given us any problems at all. I just would like to know the one that gets a lot of positive feedback. Thanks so much in advance, Stacey, HT Biomed?Group ------------------------------ Message: 6 Date: Mon, 22 Sep 2008 16:30:48 -0400 From: "Owen, Michael P" Subject: [Histonet] Seattle-Tacoma craigslist: Manager/Senior Manager, Histology and Laboratory Resources (Bothell, WA) To: "Histonet" Message-ID: <449E51C6DA0AD840B44F57C7A6EB07BF04220657@FMD3VS022.fda.gov> Content-Type: text/plain; charset="us-ascii" seattle-tacoma craigslist / jobs / biotech/sci http://seattle.craigslist.org http://seattle.craigslist.org/see/sci/850615554.html Manager/Senior Manager, Histology and Laboratory Resources (Bothell, WA) ------------------------------------------------------------------------ -------- Reply to: see below Date: 2008-09-22, 9:23AM PDT Would you like to work for a premier provider of innovative solutions in drug discovery and development? MDS Pharma Services has an exciting opportunity as a Manager/Senior Manager, Histology and Laboratory Resources at our Bothell, WA location. In this full-time position, you will be responsible for the management of our histology and bioassay laboratory and staff in support of preclinical efficacy pharmacology studies. The job is expected to be split approximately 70% managerial functions and 30% histology or bioassay laboratory bench work. Technical experience in the preparation of paraffin, plastic and/or frozen tissue specimens is required. As the laboratory manager, you will develop and administer budgets, performance requirements, and schedules. You will also monitor and control labor and capital expenditures and will interpret results of laboratory activities. You will provide operational oversight of the laboratory and technical guidance with regard to feasibility and timing to project teams. Other staff functions include interviewing, hiring, and training employees as well as addressing staff complaints and oversight of the development of new methods or assays. A BS/BA degree in a scientific field and 8+ years of experience is desired along with 2+ years of supervisory/management experience. Equivalent education and experience is also acceptable. Experience in histology and /or, histomorphometry is required. Experience with ELISA, RIA or clinical pathology laboratory procedures are highly desirable. Experience in working skeletal or CNS diseases is a plus. Familiarity with GLP and CLIA requirements are desirable as well. AA/EEO Please apply on-line at www.mdsps.com. Location: Bothell, WA Compensation: Great pay and benefits! Principals only. Recruiters, please don't contact this job poster. Please, no phone calls about this job! Please do not contact job poster about other services, products or commercial interests. PostingID: 850615554 Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov ------------------------------ Message: 7 Date: Mon, 22 Sep 2008 17:00:37 -0700 (PDT) From: Joseph Saby Subject: Re: [Histonet] Cassette Holders To: histoinfo@comcast.net, Histonet@lists.utsouthwestern.edu Message-ID: <857916.89293.qm@web33804.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Jennifer- ? Be of good cheer! ? I saw them at the NSH convention. ? Now if I could only remember where... ? Joe ----- Original Message ---- From: "histoinfo@comcast.net" To: Histonet@lists.utsouthwestern.edu Sent: Thursday, September 18, 2008 3:47:34 PM Subject: [Histonet] Cassette Holders Greetings Netters, For those of you that remember the old metal cassette holders. It seems that they are no longer available (no surprise), and I cant find anything like them on the market. They were sold by tissue-tek I think. They are about 4 inches square and one inch tall. They have 5 rows that hold 3 cassettes each for a total of 15 cassettes. We are looking for more of these, if anyone has some they are no longer using please contact me. Many Thanks, Jennifer Sauners HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 22 Sep 2008 17:11:33 -0700 (PDT) From: Jon Google Subject: [Histonet] BioCare To: "histonet@lists.utsouthwestern.edu" Message-ID: <681179.96425.qm@web59916.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Did anyone see the new biocare IHC stainer at NSH? could you give your impressions?? We are looking at the Bond, Leica Bond, but we have just heard about the biocare system. ? Thanks in advance, ------------------------------ Message: 9 Date: Mon, 22 Sep 2008 20:33:18 -0400 From: Roxanne Soto Subject: Re: [Histonet] BioCare To: "ht.ascp@yahoo.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <9E679682-16C0-43F1-B524-588DD966F603@aol.com> Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes This happens to be my favorite of all the newer instruments. If it does everything proposed-wow, what a time saver it would be. To be able to load it without the other program being finished. DAKO tried an instrument like this a couple of years ago but it bombed. I would set yourself up for a demo. Once they are released they are going to go quick- we are getting 4 of them throughout our system. Sent from my iPhone On Sep 22, 2008, at 8:11 PM, Jon Google wrote: > Did anyone see the new biocare IHC stainer at NSH? could you give > your impressions? We are looking at the Bond, Leica Bond, but we > have just heard about the biocare system. > > Thanks in advance, > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 23 Sep 2008 14:02:15 +1000 From: "Orlowski Christian" Subject: [Histonet] Cryosectioning dorsal mouse skin To: Message-ID: Content-Type: text/plain; charset="us-ascii" Greetings all, I'm wondering whether there are any recommendations for how to effectively cryosection dorsal mouse skin for immunohistochemistry. I'm having difficulty with much of the mouse skin slipping off the slide during the fixation and washing steps, which seems to result in loss of the epidermis in particular, that part of the tissue that I happen to be interested in. I apply the dorsal mouse skin cryosections onto SuperFrost Plus (Menzel-Glaser) slides and air dry them for 2 hours. I use acetone for fixation, 10 minutes, and use PBS for washing. Any suggestions? This email (including any attachments or links) may contain confidential and/or legally privileged information and is intended only to be read or used by the addressee. If you are not the intended addressee, any use, distribution, disclosure or copying of this email is strictly prohibited. Confidentiality and legal privilege attached to this email (including any attachments) are not waived or lost by reason of its mistaken delivery to you. If you have received this email in error, please delete it and notify us immediately by telephone or email. Peter MacCallum Cancer Centre provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered and will not be liable for any delay in its receipt. ------------------------------ Message: 11 Date: Tue, 23 Sep 2008 03:37:21 -0700 (PDT) From: Sohail Ejaz Subject: [Histonet] detection of brain infarcts To: histonet@lists.utsouthwestern.edu Message-ID: <551193.17390.qm@web39503.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 HI Everybody ? I am trying to find some stain that can detect brain infarct. Plreviously i was using TTC, but now we have ban on that. ? So, looking forward for a best alternative. ? Thanks ? Sohail ------------------------------ Message: 12 Date: Tue, 23 Sep 2008 12:07:08 GMT From: "mprice26@juno.com" Subject: [Histonet] Re: Blind Review Policy To: sohail_e@yahoo.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <20080923.070708.18021.0@webmail06.dca.untd.com> Content-Type: text/plain; charset=windows-1252 Does anyone have a Blind Case review Policy they could share with me? This is where when there is only one Pathologist and he does not have another pathologist to do peer review,In a one Pathologist lab. Is anyone else doing this? Thank you. Marsha Price ____________________________________________________________ Click to consolidate your debt in minutes, stop late or over-limit fees, pay less. http://thirdpartyoffers.juno.com/TGL2141/fc/Ioyw6i3m2bkZgujH4vUMxAF4GxmsePRHuCIW6Y6ywMqxb9PWv5ADBn/ ------------------------------ Message: 13 Date: Tue, 23 Sep 2008 08:37:15 -0500 From: "Ford Royer" Subject: RE: [Histonet] Cassette Holders To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I am not sure if this is what you are looking for but it is the one that I am familiar with that is still being manufactured. http://www.innovativelabacrylics.com/products/pages/cassette.html Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 Web: http://www.minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joseph Saby Sent: Monday, September 22, 2008 7:01 PM To: histoinfo@comcast.net; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cassette Holders Jennifer- ? Be of good cheer! ? I saw them at the NSH convention. ? Now if I could only remember where... ? Joe ----- Original Message ---- From: "histoinfo@comcast.net" To: Histonet@lists.utsouthwestern.edu Sent: Thursday, September 18, 2008 3:47:34 PM Subject: [Histonet] Cassette Holders Greetings Netters, For those of you that remember the old metal cassette holders. It seems that they are no longer available (no surprise), and I cant find anything like them on the market. They were sold by tissue-tek I think. They are about 4 inches square and one inch tall. They have 5 rows that hold 3 cassettes each for a total of 15 cassettes. We are looking for more of these, if anyone has some they are no longer using please contact me. Many Thanks, Jennifer Sauners HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 23 Sep 2008 06:58:05 -0700 From: "Trajkovic, Dusko" Subject: [Histonet] Effect of 5%Aeti acid on Nuclei To: Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B2080F9C1C@lajamrexm01.amer.pfizer.com> Content-Type: text/plain; charset="us-ascii" Good day to Everyone and for those that attended the NSH, WELCOME BACK. I would hope that the parties were good and I am truly sorry that I had to miss all of them. I have a friend who asked me to post this for him. Any answer or suggestion would be appreciated. Thank you Dusko Trajkovic I'm asking because in a paper I'm reviewing a paper where someone is using a concentration of 45-60% Acetic acid to fix isolated nuclei, then fixing again with Carnoy's, and then doing FISH (fluorescence in situ hyb) on them. I'm trying to figure out the effects on chromatin...does it cause the nuclei and/or chromatin to swell? I'd appreciate if you could ask around. Thanks, Yun ------------------------------ Message: 15 Date: Tue, 23 Sep 2008 07:11:22 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Effect of 5%Aeti acid on Nuclei To: Histonet@lists.utsouthwestern.edu, "Trajkovic, Dusko" Message-ID: <509501.27454.qm@web65703.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Yes, the immediate effect of acetic is nuclear swelling. Ren?? J. --- On Tue, 9/23/08, Trajkovic, Dusko wrote: From: Trajkovic, Dusko Subject: [Histonet] Effect of 5%Aeti acid on Nuclei To: Histonet@lists.utsouthwestern.edu Date: Tuesday, September 23, 2008, 9:58 AM Good day to Everyone and for those that attended the NSH, WELCOME BACK. I would hope that the parties were good and I am truly sorry that I had to miss all of them. I have a friend who asked me to post this for him. Any answer or suggestion would be appreciated. Thank you Dusko Trajkovic I'm asking because in a paper I'm reviewing a paper where someone is using a concentration of 45-60% Acetic acid to fix isolated nuclei, then fixing again with Carnoy's, and then doing FISH (fluorescence in situ hyb) on them. I'm trying to figure out the effects on chromatin...does it cause the nuclei and/or chromatin to swell? I'd appreciate if you could ask around. Thanks, Yun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 23 Sep 2008 14:47:51 GMT From: "mprice26@juno.com" Subject: Re: [Histonet] Re: Blind Review Policy To: mprice26@juno.com Cc: histonet@lists.utsouthwestern.edu, sohail_e@yahoo.com Message-ID: <20080923.094751.6345.0@webmail10.dca.untd.com> Content-Type: text/plain; charset=windows-1252 Does anyone have a policy for a Blind Case Review for a one pathologist private lab? Thank you. Marsha Price ____________________________________________________________ Internet Security Software - Click here. http://thirdpartyoffers.juno.com/TGL2141/fc/Ioyw6i3mEWrnsLu04AAPUy8TIFwZG9ifrjPeX3laAPLUa845TgE3Sp/ ------------------------------ Message: 17 Date: Tue, 23 Sep 2008 11:53:17 EDT From: HACKERLAB@aol.com Subject: Re: [Histonet] Cassette Holders To: histoinfo@comcast.net Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello Jennifer; In response to your search for cassette holders, please check out this link: _http://www.hackerinstruments.com/labstorage.htm_ (http://www.hackerinstruments.com/labstorage.htm) They were on display at the NSH meeting in Pittsburgh. Best regards, Elfi Hacker In a message dated 9/18/2008 3:48:03 P.M. Eastern Daylight Time, histoinfo@comcast.net writes: Greetings Netters, For those of you that remember the old metal cassette holders. It seems that they are no longer available (no surprise), and I cant find anything like them on the market. They were sold by tissue-tek I think. They are about 4 inches square and one inch tall. They have 5 rows that hold 3 cassettes each for a total of 15 cassettes. We are looking for more of these, if anyone has some they are no longer using please contact me. Many Thanks, Jennifer Sauners HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************Looking for simple solutions to your real-life financial challenges? Check out WalletPop for the latest news and information, tips and calculators. (http://www.walletpop.com/?NCID=emlcntuswall00000001) ------------------------------ Message: 18 Date: Tue, 23 Sep 2008 12:48:14 -0400 From: "Joyce Cline" Subject: RE: [Histonet] Ventana Retic Stain To: "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" We have to let our retic slides dry for 1 hour at 72C. We use superfrost plus slides otherwise we have variable staining or no staining. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, September 18, 2008 3:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Retic Stain Is anyone out there having problems with their Retic stain on the Ventana Nexus Special Stainer? Please contact me offline. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 58, Issue 27 **************************************** The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From rjbuesa <@t> yahoo.com Tue Sep 23 14:45:32 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 23 14:45:35 2008 Subject: [Histonet] Histology Text In-Reply-To: <137300.4313.qm@web38206.mail.mud.yahoo.com> Message-ID: <844686.40830.qm@web65708.mail.ac4.yahoo.com> Try in Amazon books Ren? J. --- On Tue, 9/23/08, Steven Coakley wrote: From: Steven Coakley Subject: [Histonet] Histology Text To: Histonet@lists.utsouthwestern.edu Date: Tuesday, September 23, 2008, 1:47 PM I'm looking for a good overall used histology text.? Maybe Carsons, Luna, or AFIP ect. ? Steve _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Tue Sep 23 14:47:19 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Sep 23 14:47:27 2008 Subject: [Histonet] Job opening Message-ID: PhenoPath Laboratories, PLLC, a pathology reference laboratory, has an opening for a Histotechnologist (full-time, M-F, 10:00 am ? 6:30 pm.) We are in a state-of-the-art facility located on the scenic Ship Canal in Seattle, Washington. Primary Responsibilities Responsibilities may include performing, at a professional level, routine manual and automated tests in a clinical and research histology laboratory. Under general supervision, perform routine laboratory tests such as processing, embedding, microtomy, H&E, special stains, etc. Required Skills/Experience Strong preference given to ASCP certified (or certification-eligible) Histotechnologists. PhenoPath Laboratories is committed to hiring the best person for the job. PhenoPath Laboratories is a national specialty pathology laboratory committed to providing outstanding clinical and research services. We offer an excellent compensation and benefits package, including 401k. Salary will be determined based on the overall skills and background of the chosen applicant. To Apply Please Contact: PhenoPath Laboratories, PLLC 551 N. 34th St., Suite 100 Seattle, WA 98103 Phone: 206 374-9000 Fax: 206 374-9009 E-mail: jobs@phenopath.com Please no phone calls about this position This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From dawn_cowie <@t> yahoo.com Tue Sep 23 14:51:20 2008 From: dawn_cowie <@t> yahoo.com (Dawn Cowie) Date: Tue Sep 23 14:51:28 2008 Subject: [Histonet] Dermatology bx. embedding guide In-Reply-To: Message-ID: <913479.24092.qm@web45016.mail.sp1.yahoo.com> Glen, I found a really good one. It's called? Dermatopathology, A Guide for the Histologist. by Clifford M. Chapman To contact the author or for ordering, call 781-246-3280 email address????? cchapman10@comcast.net website???? www.medi-sci.com ? this is a colour illustrated book, written in very easy to understand english. there is also a section on special stains pertinent to skin. ? Dawn --- On Tue, 9/23/08, Dawson, Glen wrote: From: Dawson, Glen Subject: [Histonet] Dermatology bx. embedding guide To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 23, 2008, 1:54 PM All, I am searching for an illustrated guide to embedding various derm biopsies. If anyone could forward one to me (or point me in the right direction to obtain one), I would be eternally grateful. The ideal guide would be one with pictures that could be posted next to the embedding station. Thanx in Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Tue Sep 23 15:00:47 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Sep 23 15:00:55 2008 Subject: [Histonet] ISH supplies from vendors Message-ID: I was wondering if anyone else has experienced problems in obtaining ISH probes labeled ASR? It has to do with the FDA ASR guidelines that went into effect 14Sept2008. Some vendors have definitely handled notification and supply better than others. We are having a huge issue with availability of the EBV ISH probe for the Leica Bond instrument. Just wanting to know if others are in the same boat - misery loves company, I guess. Patti Loykasek BS, HTL, QIHC Clinical IHC Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Jerry <@t> ralambusa.com Tue Sep 23 15:05:51 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Tue Sep 23 15:06:01 2008 Subject: [Histonet] RE: texas spring meeting (Horn, Hazel V) Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B5CDA@server.ralambusa.com> If I could make one humble request to Texas, Florida & California... I understand scheduling these events can be a full time job and you cannot hope to make everyone happy, but can you please try not to schedule at the same time? I want to attend all three, but have never been able to because of conflicts. Sincerely, ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 4 Date: Tue, 23 Sep 2008 12:43:58 -0500 From: "Horn, Hazel V" Subject: [Histonet] texas spring meeting To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82E28@EMAIL.archildrens.org> Content-Type: text/plain; charset="US-ASCII" If anyone from Texas can give me any details of your spring meeting '09, I would appreciate it. Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From laurie.colbert <@t> huntingtonhospital.com Tue Sep 23 15:09:37 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Sep 23 15:09:44 2008 Subject: [Histonet] ISH supplies from vendors Message-ID: <57BE698966D5C54EAE8612E8941D768303CE0EDD@EXCHANGE3.huntingtonhospital.com> Yes, we cannot get our Her2 ISH from Ventana. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Tuesday, September 23, 2008 1:01 PM To: histonet Subject: [Histonet] ISH supplies from vendors I was wondering if anyone else has experienced problems in obtaining ISH probes labeled ASR? It has to do with the FDA ASR guidelines that went into effect 14Sept2008. Some vendors have definitely handled notification and supply better than others. We are having a huge issue with availability of the EBV ISH probe for the Leica Bond instrument. Just wanting to know if others are in the same boat - misery loves company, I guess. Patti Loykasek BS, HTL, QIHC Clinical IHC Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kynemitz <@t> travmax.com Tue Sep 23 15:17:12 2008 From: kynemitz <@t> travmax.com (Kyla Nemitz) Date: Tue Sep 23 15:17:50 2008 Subject: [Histonet] Albuquerque, NM permanent Histo position Message-ID: <9416D9FA37C1C04FA83D69684E95D2E71E4DDF72@exbk2.maxhealth.com> Hello all - I just came across a perm opening in Albuquerque, NM for a Histo tech. If you or anyone you know would be interested in relocating to New Mexico, please contact me. The facility is looking for an ASCP Histo tech for a perm position. Salary is around $52,000. If you have any questions on this job or any others I have available, please contact me. Thank you in advance, hope to hear from you soon! Kyla Nemitz TravelMax Medical Professionals - Maxim Healthcare * 888.800.1855 or 813.371.5175 7 800.294.1248 www.TravelMaxAllied.com From tkngflght <@t> yahoo.com Tue Sep 23 16:41:55 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Sep 23 16:42:03 2008 Subject: [Histonet] Small lab Dayshift histologists...several states In-Reply-To: <844686.40830.qm@web65708.mail.ac4.yahoo.com> References: <137300.4313.qm@web38206.mail.mud.yahoo.com> <844686.40830.qm@web65708.mail.ac4.yahoo.com> Message-ID: <6498991352A54C4EBF497A204B8E6265@FULLSTAFF.ORG> Hi Everyone- We are seeking folks who'd love to love their job! We have full time day shift positions in several smaller labs and we're seeking stellar techs to do a fair day's work for a fair day's pay in a small lab environment where you WILL make a significant difference for your patients. We have openings in several states: Kansas - HT Florida - HT & CT Indiana - HT South Carolina - HT North Carolina - HT And our stomping grounds: Texas HT & CT (don't let a little hurricane scare ya!!) Confidential conversations welcome--our recruiter is a working histotech so it's an easy conversation (don't call Thursday morning--I'm on a Mohs bench!!) Full Staff Inc. Staffing Healthcare Professionals, one GREAT fit at a time. 800.756.3309 phone and fax resume@fullstaff.org 281.913.7285 From eyee <@t> dpmginc.com Tue Sep 23 17:07:23 2008 From: eyee <@t> dpmginc.com (Ellen Yee) Date: Tue Sep 23 17:03:55 2008 Subject: [Histonet] RE: NSH IHC Qualification Exam Class Message-ID: <20080923220723.b5855736@mail.dpmginc.com> I too would like a copy of the handouts for the NSH IHC Qualification Exam Class. Thanks, Ellen Yee, HT Central Histology Facility Diagnostic Pathology Medical Group Sacramento, CA ph: 916-448-5873 fax: 916-447-0620 From hambrightd <@t> mail.nih.gov Tue Sep 23 18:06:50 2008 From: hambrightd <@t> mail.nih.gov (Dustin Hambright) Date: Tue Sep 23 18:07:00 2008 Subject: [Histonet] CRE ANTIBODY Message-ID: Hello, Does anyone have an opinion on the best Cre antibody? I want to find out what is better - rabbit Cre antibody (Covance), rabbit Cre antibody (Novagen cat#69050), mouse Cre antibody (Chemicon). If anyone has a good working antibody, I wondered if they would be willing to share a small alliquot. We will provide Fedex account and would also like an image if possible. Thank you, Dustin Hambright NIH/NEI NNRL From relia1 <@t> earthlink.net Wed Sep 24 07:25:09 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Sep 24 07:25:15 2008 Subject: [Histonet] RELIA Histology Job Alert Exciting opportunities in Philadelphia! Message-ID: Hi Histonetters, I hope everyone is having a great week. I have a couple of new positions that I wanted to tell you about that just couldn't wait until my next Histology Careers Bulletin. I am partnering with another firm to assist a prestigious Philadelphia area dermatopathology group that is expanding. They are in need of a Histology Supervisor and a histotech. These are full time permanent positions and our client offers excellent compensation and benefits. If you or someone you know might be interested please contact me. I can be reached toll free at 866-607-3542 or relia1@earthlink.net ******Remember it never hurts to look****** Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia From hej01 <@t> health.state.ny.us Wed Sep 24 08:06:44 2008 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Wed Sep 24 08:06:55 2008 Subject: [Histonet] Indirect Immunofluorescence in Dermatopathology Message-ID: Are Indirect Immunofluorescence studies on serum samples for diagnosis of autoimmune blistering diseases considered standard tests in a Dermatopathology Lab or would it be considered a highly specialized diagnostic service (like dermatopathology, mycology, molecular diagnostic, etc.) under Dermatology? When reporting results, do you need a disclaimer that it is for reseach use only and not approved by FDA? Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From yeonju.shim <@t> novartis.com Wed Sep 24 09:42:52 2008 From: yeonju.shim <@t> novartis.com (yeonju.shim@novartis.com) Date: Wed Sep 24 09:43:01 2008 Subject: [Histonet] neo-clear Message-ID: Hi, I would like to know if there's anyone using neo-clear for processing and how good/bad it is. Thank you so much! Judy _________________________ CONFIDENTIALITY NOTICE The information contained in this e-mail message is intended only for the exclusive use of the individual or entity named above and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivery of the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by e-mail and delete the material from any computer. Thank you. From GDeMatteo <@t> srhs.org Wed Sep 24 12:36:24 2008 From: GDeMatteo <@t> srhs.org (DeMatteo, Gabriela) Date: Wed Sep 24 12:38:54 2008 Subject: FW: [Histonet] RE: NSH IHC Qualification Exam Class References: <81418DA27DB07A4FB89264117C6EA4989FBD008DAB@LC-HQEXCH01.lifecell.biz> Message-ID: I could not attend the NSH meeting due to staffing. I was also hoping if someone could help me get a hold of the handouts for the QIHC exam class Thank you. Gabriela ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Garfield, Jacqueline Sent: Tue 9/23/2008 1:50 PM To: 'Evans, Andria B.'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: NSH IHC Qualification Exam Class I was in the same boat. If anyone is willing to share, I greatly appreciate it. Sincerely, Jackie Garfield Jacqueline D. Garfield | Manager, Histology Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1182 Email jgarfield@ lifecell.com www.lifcell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B. Sent: Tuesday, September 23, 2008 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH IHC Qualification Exam Class I was wondering if anyone could send me the handouts from the IHC Qualification class that was presented at the NSH Convention? I wanted to take that class, but ended up taking others. Please email me if you have them. Thanks! Andria B Evans, HTL(ASCP)CM CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *********************************************************************************************************************************************** This e-mail message and any attachments are confidential. Dissemination, distribution or copying of this e-mail or any attachments by anyone other than the intended recipient is prohibited. If you are not the intended recipient, please notify LifeCell Corporation immediately by replying to this e-mail, and destroy all copies of this e-mail and any attachments. Thank you! *********************************************************************************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amurray <@t> nyp.org Wed Sep 24 12:40:10 2008 From: amurray <@t> nyp.org (Angela R. Murray) Date: Wed Sep 24 12:42:23 2008 Subject: [Histonet] Bone Marrow Biopsy Fixation Message-ID: <48DA7B7A.4050903@nyp.org> Have you fixed bone marrow biopsies in formaling and if so for how long and what decal solution is best? -------------------- This electronic message is intended to be for the use only of the named recipient, and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. Thank you. From RSRICHMOND <@t> aol.com Wed Sep 24 12:49:54 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Sep 24 12:49:58 2008 Subject: [Histonet] Re: Neo-Clear Message-ID: There have been several inquiries on this list about Neo-Clear, an aliphatic type (not a limonene) xylene substitute that is offered under the old Harleco label. (I'm not sure who owns Harleco these days.) There is a specific mounting medium (Neo-Mount) offered with it. The MSDS is unclear about the flash point of Neo-Clear, though it's higher than xylene's. There are numerous brands of aliphatic xylene substitutes, and they aren't always interchangeable with each other, particularly in distillation routines for recycling. Whoever it is makes and markets Neo-Clear, I have no commercial connection with them. Bob Richmond Samurai Pathologist Knoxville TN From trathborne <@t> somerset-healthcare.com Wed Sep 24 13:00:12 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Sep 24 13:00:23 2008 Subject: [Histonet] Tissue processors/Recyclers Message-ID: We are currently exploring the advantages of recycling our waste solutions as well as a new tissue processor. The two processors we are looking into are the Peloris from Leica and the Sakura Xpress. I would appreciate hearing the advantages and disadvantages of each, and if anyone is recycling the Sakura processing reagent or the Leica Waxsol. If vendors of recyclers are out there, please feel free to answer regarding the solutions. Toni Rathborne Somerset Medical Center CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From ktuttle <@t> umm.edu Wed Sep 24 13:01:32 2008 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Wed Sep 24 13:01:53 2008 Subject: [Histonet] Dako Party In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A70208C213@LTA3VS011.ees.hhs.gov> References: <1CE1847DFEA0A647B1CCDE4108EA60A70208C213@LTA3VS011.ees.hhs.gov> Message-ID: <48DA483C.90CE.001A.3@umm.edu> Andria, I have some photos, and since I took the photos, Im not in any of them. I had 1 too many Chardonnay but thankfully that was my last night, so I didn't have to face yall the next day. Can you send photos on histonet or must I send them directly to your email? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 9/23/2008 1:42 pm >>> I think all of the pictures have been confiscated......to protect the not-so-innocent. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B. Sent: Tuesday, September 23, 2008 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Party I was wondering if anyone has any pictures of the Dako Party from the NSH Convention. I dropped my camera the night before and broke it and was not able to take pictures. I would be willing to send a CD for the pictures to be writtin to along with postage to send it back to me. Please email me if you can help. Thanks! Andria B Evans, HTL(ASCP)CM CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From AJohnson <@t> aipathology.com Wed Sep 24 13:39:15 2008 From: AJohnson <@t> aipathology.com (Amy Johnson) Date: Wed Sep 24 13:39:21 2008 Subject: [Histonet] Gi Biopsies and the Tissue Tek VIP Message-ID: <4D6F15688B0DD34AA60F1C9A076BE57C292475@SERV001> Can anyone out there help me figure out what is the shortest amount of time you can process GI biopsies with a Tissue Tek VIP? Thanks Amy Johnson Associates in Pathology From thisisann <@t> aol.com Wed Sep 24 14:26:37 2008 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Wed Sep 24 14:27:05 2008 Subject: [Histonet] CAP Question Message-ID: <8CAEC9298BB6A1C-790-1040@FWM-M33.sysops.aol.com> I am going through the CAP checklist and came upon this question: ANP23075:? Is there evidence of ongoing evaluation of results of instrumetn maintenance and function for all devices? The question before it asks if service reports are kept and they are.? How do I respond to this question other than the equipment works after being serviced!!!? Any ideas? Thank you, Ann From christiegowan <@t> msn.com Wed Sep 24 14:28:51 2008 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Wed Sep 24 14:28:59 2008 Subject: [Histonet] NSH IHC Qualification Exam Class In-Reply-To: Message-ID: Ethel Macrea taught the class at NSH and if you want the handout you can email her directly at dawnbrok@earthlink.net. She no longer subscribes to the histonet so was not aware of these requests. >From: "Evans, Andria B." >To: >Subject: [Histonet] NSH IHC Qualification Exam Class >Date: Tue, 23 Sep 2008 13:28:13 -0400 > >I was wondering if anyone could send me the handouts from the IHC >Qualification class that was presented at the NSH Convention? I wanted to >take that class, but ended up taking others. Please email me if you have >them. Thanks! > >Andria B Evans, HTL(ASCP)CM > >CONFIDENTIALITY NOTICE: > >This email may contain confidential health information that is legally >privileged. This information is intended for the use of the named >recipient(s). The authorized recipient of this information is prohibited >from disclosing this information to any party unless required to do so by >law or regulation and is required to destroy the information after its >stated need has been fulfilled. If you are not the intended recipient, you >are hereby notified that any disclosure, copying, distribution, or action >taken in reliance on the contents of this email is strictly prohibited. If >you receive this e-mail message in error, please notify the sender >immediately to arrange disposition of the information. > > >______________________________________________________________________ >This e-mail has been scanned by MCI Managed Email Content Service, using >Skeptic(tm) technology powered by MessageLabs. For more information on >MCI's Managed Email Content Service, visit http://www.mci.com. >______________________________________________________________________ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Sep 24 14:44:27 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 24 14:44:31 2008 Subject: [Histonet] Indirect Immunofluorescence in Dermatopathology Message-ID: <993690.22771.qm@web65715.mail.ac4.yahoo.com> For years we did IDF studies (indirect) using serum and no disclaimer was ever used. Pemphigus vulgaris and Bullus pemphigoid and related disorders are diagnosed, and even the serum in titred for intensity, as standard procedure. Ren? J. --- On Wed, 9/24/08, Helen E Johnson wrote: From: Helen E Johnson Subject: [Histonet] Indirect Immunofluorescence in Dermatopathology To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 24, 2008, 9:06 AM Are Indirect Immunofluorescence studies on serum samples for diagnosis of autoimmune blistering diseases considered standard tests in a Dermatopathology Lab or would it be considered a highly specialized diagnostic service (like dermatopathology, mycology, molecular diagnostic, etc.) under Dermatology? When reporting results, do you need a disclaimer that it is for reseach use only and not approved by FDA? Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Sep 24 14:45:46 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 24 14:45:49 2008 Subject: [Histonet] Bone Marrow Biopsy Fixation In-Reply-To: <48DA7B7A.4050903@nyp.org> Message-ID: <540061.21112.qm@web65712.mail.ac4.yahoo.com> NBF was our standard fixative for BM biopsies, BUT you have to be sure that the pH=7 For decalcification we used EDTA Ren? J. --- On Wed, 9/24/08, Angela R. Murray wrote: From: Angela R. Murray Subject: [Histonet] Bone Marrow Biopsy Fixation To: Histonet@lists.utsouthwestern.edu Date: Wednesday, September 24, 2008, 1:40 PM Have you fixed bone marrow biopsies in formaling and if so for how long and what decal solution is best? -------------------- This electronic message is intended to be for the use only of the named recipient, and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Sep 24 14:49:53 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 24 14:49:56 2008 Subject: [Histonet] Re: Neo-Clear In-Reply-To: Message-ID: <422204.70642.qm@web65706.mail.ac4.yahoo.com> Neo-Clear, a mixture of C9-C10 aliphatic hydrocarbons is a trade name for the common white spirits used in dry cleaning, meaning that it is 100% Stoddard solvent (CAS#8052-41-3). Ren? J. --- On Wed, 9/24/08, Robert Richmond wrote: From: Robert Richmond Subject: [Histonet] Re: Neo-Clear To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 24, 2008, 1:49 PM There have been several inquiries on this list about Neo-Clear, an aliphatic type (not a limonene) xylene substitute that is offered under the old Harleco label. (I'm not sure who owns Harleco these days.) There is a specific mounting medium (Neo-Mount) offered with it. The MSDS is unclear about the flash point of Neo-Clear, though it's higher than xylene's. There are numerous brands of aliphatic xylene substitutes, and they aren't always interchangeable with each other, particularly in distillation routines for recycling. Whoever it is makes and markets Neo-Clear, I have no commercial connection with them. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Wed Sep 24 14:49:58 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Sep 24 14:50:16 2008 Subject: [Histonet] CAP Question In-Reply-To: <8CAEC9298BB6A1C-790-1040@FWM-M33.sysops.aol.com> References: <8CAEC9298BB6A1C-790-1040@FWM-M33.sysops.aol.com> Message-ID: <48DA61A6.2B7F.00C9.0@geisinger.edu> I sign off on all of our daily maintenance/temperature charts at the end of each month. I interpret in this question that "instrument maintenance " means the daily or monthly maintenance that we do ourselves in the lab. Such as, changing solutions on our processors and stainers. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> 9/24/2008 3:26 PM >>> I am going through the CAP checklist and came upon this question: ANP23075:? Is there evidence of ongoing evaluation of results of instrumetn maintenance and function for all devices? The question before it asks if service reports are kept and they are.? How do I respond to this question other than the equipment works after being serviced!!!? Any ideas? Thank you, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From christiegowan <@t> msn.com Wed Sep 24 14:57:22 2008 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Wed Sep 24 14:57:28 2008 Subject: [Histonet] CAP Question In-Reply-To: <48DA61A6.2B7F.00C9.0@geisinger.edu> Message-ID: I agree with Angela and this has always been ok for my CAP inspections. >From: "Angela Bitting" >To: , >Subject: Re: [Histonet] CAP Question >Date: Wed, 24 Sep 2008 15:49:58 -0400 > >I sign off on all of our daily maintenance/temperature charts at the end of >each month. I interpret in this question that "instrument maintenance " >means the daily or monthly maintenance that we do ourselves in the lab. >Such as, changing solutions on our processors and stainers. > >Angela Bitting, HT(ASCP) >Technical Specialist, Histology >Geisinger Medical Center >100 N Academy Ave. MC 23-00 >Danville, PA 17822 >phone 570-214-9634 >fax 570-271-5916 > >No trees were hurt in the sending of this email >However many electrons were severly inconvienienced! > > > >>> 9/24/2008 3:26 PM >>> >I am going through the CAP checklist and came upon this question: > >ANP23075:? Is there evidence of ongoing evaluation of results of instrumetn >maintenance and function for all devices? > >The question before it asks if service reports are kept and they are.? How >do I respond to this question other than the equipment works after being >serviced!!!? Any ideas? > >Thank you, >Ann >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >IMPORTANT WARNING: The information in this message (and the documents >attached to it, if any) is confidential and may be legally privileged. It >is intended solely for the addressee. Access to this message by anyone else >is unauthorized. If you are not the intended recipient, any disclosure, >copying, distribution or any action taken, or omitted to be taken, in >reliance on it is prohibited and may be unlawful. If you have received this >message in error, please delete all electronic copies of this message (and >the documents attached to it, if any), destroy any hard copies you may have >created and notify me immediately by replying to this email. Thank you. ><< AngelaBitting1.vcf >> >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Sep 24 14:58:36 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 24 14:58:38 2008 Subject: [Histonet] Gi Biopsies and the Tissue Tek VIP In-Reply-To: <4D6F15688B0DD34AA60F1C9A076BE57C292475@SERV001> Message-ID: <220681.66424.qm@web65708.mail.ac4.yahoo.com> 2.5 hours Ren? J. --- On Wed, 9/24/08, Amy Johnson wrote: From: Amy Johnson Subject: [Histonet] Gi Biopsies and the Tissue Tek VIP To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 24, 2008, 2:39 PM Can anyone out there help me figure out what is the shortest amount of time you can process GI biopsies with a Tissue Tek VIP? Thanks Amy Johnson Associates in Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Sep 24 15:01:18 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 24 15:01:23 2008 Subject: [Histonet] CAP Question In-Reply-To: <8CAEC9298BB6A1C-790-1040@FWM-M33.sysops.aol.com> Message-ID: <597921.87557.qm@web65701.mail.ac4.yahoo.com> You should have your instruments checked on a regular basis. In our hospital we used the services of our?engineering department, but you can contract a specialized company to do that. After each general maintenance, the results are logged in a special form you develop for each instrument. Ren? J. --- On Wed, 9/24/08, thisisann@aol.com wrote: From: thisisann@aol.com Subject: [Histonet] CAP Question To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 24, 2008, 3:26 PM I am going through the CAP checklist and came upon this question: ANP23075:? Is there evidence of ongoing evaluation of results of instrumetn maintenance and function for all devices? The question before it asks if service reports are kept and they are.? How do I respond to this question other than the equipment works after being serviced!!!? Any ideas? Thank you, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aevans <@t> wellspan.org Wed Sep 24 15:02:54 2008 From: aevans <@t> wellspan.org (Evans, Andria B.) Date: Wed Sep 24 15:03:32 2008 Subject: [Histonet] Histology Tech Position - York, Pennsylvania Message-ID: Hi Everyone! We are currently looking for a Full Time Histology Technologist to fill a current opening. We are located in York, Pennsylvania. See our posting on www.wellspan.org Andria B Evans, HTL(ASCP)CM CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From rjbuesa <@t> yahoo.com Wed Sep 24 15:08:20 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Sep 24 15:08:23 2008 Subject: [Histonet] Tissue processors/Recyclers Message-ID: <504339.59672.qm@web65704.mail.ac4.yahoo.com> Peloris and Xpress are two completely different technologies. Peloris is a conventional? tissue processor with the capability of rapidly increasing the temperature to boil the 2-propanol at low pressure and 85?C (if you want to avoid using xylene). Xpress has half the retorts (2 or 1 depending on the model) as a microwave assisted chamber, and the other half as a conventional tissue processor. The differences in price are also high. Peloris has two chambers and operate quite conventionally, Xpress is designed to have a constant flow of processed blocks every 1-2 hours. The impact on the schedule and working conditions of the laboratory makes them completely different instru Ren? J. --- On Wed, 9/24/08, Rathborne, Toni wrote: From: Rathborne, Toni Subject: [Histonet] Tissue processors/Recyclers To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 24, 2008, 2:00 PM We are currently exploring the advantages of recycling our waste solutions as well as a new tissue processor. The two processors we are looking into are the Peloris from Leica and the Sakura Xpress. I would appreciate hearing the advantages and disadvantages of each, and if anyone is recycling the Sakura processing reagent or the Leica Waxsol. If vendors of recyclers are out there, please feel free to answer regarding the solutions. Toni Rathborne Somerset Medical Center CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotechkb <@t> gmail.com Wed Sep 24 15:11:46 2008 From: histotechkb <@t> gmail.com (Kristen Yaros) Date: Wed Sep 24 15:11:50 2008 Subject: [Histonet] HTL Flash Cards? Message-ID: <667c97ab0809241311w517e21d3i2a151f9ae342fc8e@mail.gmail.com> Has anyone heard of these? I just came across this site and was wondering if anyone has used these pre-made flash cards. http://www.flashcardsecrets.com/histotech/ -- Kristen Yaros, HT (ASCP)CM Histotechnology Society of Delaware Correspondence Secretary histotechkb@gmail.com From JWeems <@t> sjha.org Wed Sep 24 15:26:12 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Sep 24 15:26:23 2008 Subject: [Histonet] CAP Question In-Reply-To: <48DA61A6.2B7F.00C9.0@geisinger.edu> References: <8CAEC9298BB6A1C-790-1040@FWM-M33.sysops.aol.com> <48DA61A6.2B7F.00C9.0@geisinger.edu> Message-ID: <982A0A9461F9BF438C7B19A6E425A38366AA8A@ITSSSXM01V6.one.ads.che.org> I take this to mean the yearly maintainence checks and any instrument repair that has been done. Our Biomed dept does an annual electrical check on each instrument and keeps those records in their department, as well as any repairs they do on our instruments. For any instruments we have service contracts for, I keep records of annual PMs and any repairs in my office. We keep the the daily maintenance/temp charts as QC records. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, September 24, 2008 3:50 PM To: thisisann@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CAP Question I sign off on all of our daily maintenance/temperature charts at the end of each month. I interpret in this question that "instrument maintenance " means the daily or monthly maintenance that we do ourselves in the lab. Such as, changing solutions on our processors and stainers. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> 9/24/2008 3:26 PM >>> I am going through the CAP checklist and came upon this question: ANP23075:? Is there evidence of ongoing evaluation of results of instrumetn maintenance and function for all devices? The question before it asks if service reports are kept and they are.? How do I respond to this question other than the equipment works after being serviced!!!? Any ideas? Thank you, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From laurie.colbert <@t> huntingtonhospital.com Wed Sep 24 15:51:38 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Sep 24 15:51:43 2008 Subject: [Histonet] Gi Biopsies and the Tissue Tek VIP Message-ID: <57BE698966D5C54EAE8612E8941D768303CE11D6@EXCHANGE3.huntingtonhospital.com> We have all stations set at 10 minutes, except for the last xylene and the last two paraffins - which are 15 minutes each. We actually have the two formalins at one hour and one and a half hours, in case someone mistakenly puts the big tissue on the bx processor - at least they will be well-fixed before we have to re-process them. We have plenty of time to have the formalin at longer times, but if your specimens are already fixed, you should be able to go with the 10 minutes. Laurie Colbert Huntington Hospital (626) 397-8620 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, September 24, 2008 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gi Biopsies and the Tissue Tek VIP Can anyone out there help me figure out what is the shortest amount of time you can process GI biopsies with a Tissue Tek VIP? Thanks Amy Johnson Associates in Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Wed Sep 24 16:29:49 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Sep 24 16:30:37 2008 Subject: [Histonet] Tissue processors/Recyclers In-Reply-To: References: Message-ID: Toni, Check out Creative Waste Solutions who have gravity feed alcohol and formalin recycling solutions. Call Rex at: 888-795-8300 or 503-963-8037. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, September 24, 2008 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue processors/Recyclers We are currently exploring the advantages of recycling our waste solutions as well as a new tissue processor. The two processors we are looking into are the Peloris from Leica and the Sakura Xpress. I would appreciate hearing the advantages and disadvantages of each, and if anyone is recycling the Sakura processing reagent or the Leica Waxsol. If vendors of recyclers are out there, please feel free to answer regarding the solutions. Toni Rathborne Somerset Medical Center CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.169 / Virus Database: 270.7.1/1688 - Release Date: 9/24/2008 6:29 AM From joelleweaver <@t> hotmail.com Wed Sep 24 18:18:30 2008 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Sep 24 18:18:34 2008 Subject: [Histonet] comments/opinions on Shandon Gemini H & E stainer Message-ID: Hello everyone- I wanted to see if anyone was willing to share any opinions, experiences or assessments of the Shandon Gemini stainer? This instrument is being considered for purchase by my laboratory. It will be primarily used for routine H & E staining and some cytology staining. We may only have limited ability to demo, so I wanted to see if anyone had anything that they might be willing to share regarding the pros and cons of this instrument. I have not personally used this instrument or many of its design style- so any information would be extremely helpful! Thanks so much! Joelle Weaver HTL(ASCP) _________________________________________________________________ Want to do more with Windows Live? Learn ?10 hidden secrets? from Jamie. http://windowslive.com/connect/post/jamiethomson.spaces.live.com-Blog-cns!550F681DAD532637!5295.entry?ocid=TXT_TAGLM_WL_domore_092008 From joelleweaver <@t> hotmail.com Wed Sep 24 18:25:06 2008 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Sep 24 18:25:09 2008 Subject: [Histonet] HTL Flash Cards? In-Reply-To: <667c97ab0809241311w517e21d3i2a151f9ae342fc8e@mail.gmail.com> References: <667c97ab0809241311w517e21d3i2a151f9ae342fc8e@mail.gmail.com> Message-ID: Hi Kristen Thanks for sharing this interesting site. I think that it might be useful now that exam is a computer-adapted test model. I think that these could be helpful for review. The topic list seemed fairly complete. I have never personally used them. I just studied from my own notes and reference texts, but I think that I will keep these in mind if any students in our local histotechnology program asks about study materials. Regards- Joelle Weaver HTL(ASCP)> Date: Wed, 24 Sep 2008 16:11:46 -0400> From: histotechkb@gmail.com> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] HTL Flash Cards?> > Has anyone heard of these? I just came across this site and was wondering if> anyone has used these pre-made flash cards.> > http://www.flashcardsecrets.com/histotech/> > -- > Kristen Yaros, HT (ASCP)CM> Histotechnology Society of Delaware> Correspondence Secretary> histotechkb@gmail.com> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Want to do more with Windows Live? Learn ?10 hidden secrets? from Jamie. http://windowslive.com/connect/post/jamiethomson.spaces.live.com-Blog-cns!550F681DAD532637!5295.entry?ocid=TXT_TAGLM_WL_domore_092008 From eileen_dusek <@t> yahoo.com Wed Sep 24 18:28:57 2008 From: eileen_dusek <@t> yahoo.com (eileen dusek) Date: Wed Sep 24 18:29:03 2008 Subject: [Histonet] Job opening at University of Chicago Message-ID: <930694.4411.qm@web50601.mail.re2.yahoo.com> Hello all, ? We currently have two job openings in the Histology Laboratory at The University of Chicago Medical Center: ? Chief Medical Technologist: ? ASCP certified (HT or HTL) with a minimum 5 years experience with at least 3 years in supervisory role in a histology laboratory, preferably at an academic medical center.? Duties include the daily supervision and management (17 FTEs) of the histology laboratory including renal and neuropath specialties and the electron microscopy laboratory.? The ideal candidate not only will be able to handle the daily job duties of a histology laboratory supervisor but also desires an opportunity to evaluate and implement new technologies/diagnostic services and be one of the team leaders in helping the UCMC Anatomic Pathology Laboratory achieve its immediate and future goals. ? Please reference job # JB19978 ? Senior Histotechnologist ?Looking for a team player who is ASCP certified (HT or HTL) with a minimum of three years experience.? Duties include all routine histology functions in an academic environment (i.e. embedding, cutting, & special stains).? The successful candidate will have the opportunity to learn and become a specialist in at least one of the following histology services; renal path, neuro path, and/or electron microscopy.? ? Please reference job #JB17800 ? If interested please visit us at http://www.uchospitals.edu/jobs/index.html and search under Branch: Laboratory.? If questions, please call Eileen Dusek at (773) 702-9052. ? The University of Chicago Medical Center is proud to be an EEO/AA employer M/F/D/V. We maintain a drug-free workplace and perform pre-employment substance abuse testing. From marilynng <@t> earthlink.net Wed Sep 24 18:55:38 2008 From: marilynng <@t> earthlink.net (Marilyn Gamble) Date: Wed Sep 24 18:55:43 2008 Subject: [Histonet] QHIC Qualification Exam Message-ID: <380-220089324235538984@earthlink.net> During the the NSH meeting in Pittsburgh, I responded to a few questions/misunderstandings about the QHIC examination. I'd like to share my responses with the Histonet community: You do not have to be ASCP Certified to take the Qualification examination. Individuals who work in research laboratories are eligible to take the Qualification examination if they work in the U.S. or Canada and meet the education or certification and experience requirements as defined in the selected Route. Individuals who work outside of the U.S. or Canada may qualify if they work in a CAP, JCAHO, or AABB accredited laboratory and meet the education or certification and experience requirements as defined in the selected Route. You can find complete information on the Qualification at www.ascp.org Marilyn Gamble marilynng@earthlink.net From dshtilki <@t> uci.edu Wed Sep 24 19:10:27 2008 From: dshtilki <@t> uci.edu (Shtilkind, Dmitriy) Date: Wed Sep 24 19:10:33 2008 Subject: [Histonet] Job opening Message-ID: We have a Histotechnologist III opening @ University of California, Irvine Medical Center. If interested you can register here https://staffing2.hr.uci.edu/CSS_External/CSSPage_Welcome.asp and then apply. Dmitriy Shtilkind, HTL (ASCP) Histology Supervisor UCI Medical Center Orange, CA, 92868 (714) 456-8731 dshtilki@uci.edu ________________________________ This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. From slappycraw <@t> yahoo.com Wed Sep 24 19:42:29 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Wed Sep 24 19:42:33 2008 Subject: [Histonet] comments/opinions on Shandon Gemini H & E stainer In-Reply-To: Message-ID: <497288.92266.qm@web53608.mail.re2.yahoo.com> Put down the kool-aid and get as far away from that intsrument as possible and I'm not kidding. Larry A. Woody Seattle, Wa. --- On Wed, 9/24/08, joelle weaver wrote: > From: joelle weaver > Subject: [Histonet] comments/opinions on Shandon Gemini H & E stainer > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, September 24, 2008, 4:18 PM > Hello everyone- > I wanted to see if anyone was willing to share any > opinions, experiences or assessments of the Shandon Gemini > stainer? This instrument is being considered for purchase by > my laboratory. It will be primarily used for routine H & > E staining and some cytology staining. We may only have > limited ability to demo, so I wanted to see if anyone had > anything that they might be willing to share regarding the > pros and cons of this instrument. I have not personally used > this instrument or many of its design style- so any > information would be extremely helpful! > Thanks so much! > Joelle Weaver HTL(ASCP) > _________________________________________________________________ > Want to do more with Windows Live? Learn ?10 hidden > secrets? from Jamie. > http://windowslive.com/connect/post/jamiethomson.spaces.live.com-Blog-cns!550F681DAD532637!5295.entry?ocid=TXT_TAGLM_WL_domore_092008_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> hotmail.com Wed Sep 24 22:22:06 2008 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Sep 24 22:22:11 2008 Subject: [Histonet] Tissue processors/Recyclers In-Reply-To: <504339.59672.qm@web65704.mail.ac4.yahoo.com> References: <504339.59672.qm@web65704.mail.ac4.yahoo.com> Message-ID: I have been a user of the Xpress for four + years. You first have to decie what is the primary reason to move to rapid tissue processing. Do you require continuous load or batch loading? do you require loading w/out a clanining cylcle? my experience has directed me to using the Xpress because you can coninually load cassettes w/out waiting for a cleaning cycle to complete before loading another set of cassettes. I recommend the Xpress for the cotinuing load capability. > Date: Wed, 24 Sep 2008 13:08:20 -0700> From: rjbuesa@yahoo.com> To: histonet@lists.utsouthwestern.edu; trathborne@somerset-healthcare.com> Subject: Re: [Histonet] Tissue processors/Recyclers> CC: > > > Peloris and Xpress are two completely different technologies. Peloris is a conventional tissue processor with the capability of rapidly increasing the temperature to boil the 2-propanol at low pressure and 85?C (if you want to avoid using xylene).> Xpress has half the retorts (2 or 1 depending on the model) as a microwave assisted chamber, and the other half as a conventional tissue processor. The differences in price are also high. Peloris has two chambers and operate quite conventionally, Xpress is designed to have a constant flow of processed blocks every 1-2 hours. The impact on the schedule and working conditions of the laboratory makes them completely different instru> Ren? J.> > --- On Wed, 9/24/08, Rathborne, Toni wrote:> > From: Rathborne, Toni > Subject: [Histonet] Tissue processors/Recyclers> To: histonet@lists.utsouthwestern.edu> Date: Wednesday, September 24, 2008, 2:00 PM> > We are currently exploring the advantages of recycling our waste solutions as> well as a new tissue processor. The two processors we are looking into are the> Peloris from Leica and the Sakura Xpress. I would appreciate hearing the> advantages and disadvantages of each, and if anyone is recycling the Sakura> processing reagent or the Leica Waxsol. If vendors of recyclers are out there,> please feel free to answer regarding the solutions.> > Toni Rathborne> Somerset Medical Center> > > CONFIDENTIALITY NOTICE> This message and any included attachments are from Somerset Medical Center> and are intended only for the addressee. The information contained in this> message is confidential and may contain privileged, confidential,> proprietary and/or trade secret information entitled to protection and/or> exemption from disclosure under applicable law. Unauthorized forwarding,> printing, copying, distribution, or use of such information is strictly> prohibited and may be unlawful. If you are not the addressee, please> promptly delete this message and notify the sender of the delivery error> by e-mail or you may call Somerset Medical Center's computer Help Desk> at 908-685-2200, ext. 4050.> > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more.> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Get more out of the Web. Learn 10 hidden secrets of Windows Live. http://windowslive.com/connect/post/jamiethomson.spaces.live.com-Blog-cns!550F681DAD532637!5295.entry?ocid=TXT_TAGLM_WL_domore_092008 From wdesalvo.cac <@t> hotmail.com Wed Sep 24 22:24:11 2008 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Sep 24 22:24:14 2008 Subject: [Histonet] CAP Question In-Reply-To: <597921.87557.qm@web65701.mail.ac4.yahoo.com> References: <8CAEC9298BB6A1C-790-1040@FWM-M33.sysops.aol.com> <597921.87557.qm@web65701.mail.ac4.yahoo.com> Message-ID: I suggest that you use your biomedical depatment. You need to establish a consistent process and then stick to it. > Date: Wed, 24 Sep 2008 13:01:18 -0700> From: rjbuesa@yahoo.com> To: histonet@lists.utsouthwestern.edu; thisisann@aol.com> Subject: Re: [Histonet] CAP Question> CC: > > You should have your instruments checked on a regular basis. In our hospital we used the services of our engineering department, but you can contract a specialized company to do that. After each general maintenance, the results are logged in a special form you develop for each instrument.> Ren? J.> > --- On Wed, 9/24/08, thisisann@aol.com wrote:> > From: thisisann@aol.com > Subject: [Histonet] CAP Question> To: histonet@lists.utsouthwestern.edu> Date: Wednesday, September 24, 2008, 3:26 PM> > I am going through the CAP checklist and came upon this question:> > ANP23075:? Is there evidence of ongoing evaluation of results of instrumetn> maintenance and function for all devices?> > The question before it asks if service reports are kept and they are.? How do I> respond to this question other than the equipment works after being serviced!!!?> Any ideas?> > Thank you,> Ann> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ See how Windows connects the people, information, and fun that are part of your life. http://clk.atdmt.com/MRT/go/msnnkwxp1020093175mrt/direct/01/ From gu.lang <@t> gmx.at Thu Sep 25 01:44:35 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Sep 25 01:44:53 2008 Subject: AW: [Histonet] Gi Biopsies and the Tissue Tek VIP In-Reply-To: <57BE698966D5C54EAE8612E8941D768303CE11D6@EXCHANGE3.huntingtonhospital.com> Message-ID: <156AF707500D498780C145D357BDD49D@dielangs.at> Laurie, do you use the biopsy-sponges with this protocol? And how many cassettes do you process at one time? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Laurie Colbert Gesendet: Mittwoch, 24. September 2008 22:52 An: Amy Johnson Cc: histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Gi Biopsies and the Tissue Tek VIP We have all stations set at 10 minutes, except for the last xylene and the last two paraffins - which are 15 minutes each. We actually have the two formalins at one hour and one and a half hours, in case someone mistakenly puts the big tissue on the bx processor - at least they will be well-fixed before we have to re-process them. We have plenty of time to have the formalin at longer times, but if your specimens are already fixed, you should be able to go with the 10 minutes. Laurie Colbert Huntington Hospital (626) 397-8620 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, September 24, 2008 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gi Biopsies and the Tissue Tek VIP Can anyone out there help me figure out what is the shortest amount of time you can process GI biopsies with a Tissue Tek VIP? Thanks Amy Johnson Associates in Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Sep 25 01:52:34 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Sep 25 01:52:59 2008 Subject: AW: [Histonet] Bone Marrow Biopsy Fixation In-Reply-To: <48DA7B7A.4050903@nyp.org> Message-ID: <826ED0CE3E1C4D2CAAFB891F7373B85B@dielangs.at> Angela, we use NBF for at least 16 hours, the next day after receiving they are put in decal (mixture of formic acid and hydrochloric acid with formaldehyd) for 7-8 hours. Our biopsies are about 3-4 mm thick. After acid decalcification enzyme-stains are not possible any more and the iron-stain could be false negative. But we have good experiences with the IHC and had even an improvement with some markers compared with EDTA-decal. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Angela R. Murray Gesendet: Mittwoch, 24. September 2008 19:40 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Bone Marrow Biopsy Fixation Have you fixed bone marrow biopsies in formaling and if so for how long and what decal solution is best? -------------------- This electronic message is intended to be for the use only of the named recipient, and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. Thank you. From gu.lang <@t> gmx.at Thu Sep 25 01:58:17 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Sep 25 01:58:34 2008 Subject: AW: [Histonet] comments/opinions on Shandon Gemini H & E stainer In-Reply-To: Message-ID: <1093B2FBCB194E86A830394CA2820CC8@dielangs.at> Joelle, we had a demo on this instrument. Our staining protocol was easy set up, it is a rapid machine. The software was smart, because the stainer always searches for the fastest way. The only drawback was, that at that time, there was no coverslipper to be attached to the stainer. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von joelle weaver Gesendet: Donnerstag, 25. September 2008 01:19 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] comments/opinions on Shandon Gemini H & E stainer Hello everyone- I wanted to see if anyone was willing to share any opinions, experiences or assessments of the Shandon Gemini stainer? This instrument is being considered for purchase by my laboratory. It will be primarily used for routine H & E staining and some cytology staining. We may only have limited ability to demo, so I wanted to see if anyone had anything that they might be willing to share regarding the pros and cons of this instrument. I have not personally used this instrument or many of its design style- so any information would be extremely helpful! Thanks so much! Joelle Weaver HTL(ASCP) _________________________________________________________________ Want to do more with Windows Live? Learn ?10 hidden secrets? from Jamie. http://windowslive.com/connect/post/jamiethomson.spaces.live.com-Blog-cns!55 0F681DAD532637!5295.entry?ocid=TXT_TAGLM_WL_domore_092008___________________ ____________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JGREWE <@t> OhioHealth.com Thu Sep 25 04:11:52 2008 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Thu Sep 25 04:12:00 2008 Subject: [Histonet] Immunoperoxidase Control Tissue Message-ID: I am interested in finding sources that sell control tissue for immunoperoxidase and special stains. Does anyone have any suggestions? Thanks Jacquelyn L. Grewe BS, HT (ASCP) Laboratory Supervisor, Anatomic Pathology Riverside Methodist Hospital 614-566-4562 or 614-566-5679 _______________________________________________ FORTUNE "100 Best Companies to Work for 2008 " From rfields <@t> gidocs.net Thu Sep 25 07:14:36 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Thu Sep 25 07:15:50 2008 Subject: [Histonet] comments/opinions on Shandon Gemini H & E stainer Message-ID: <07732CE52EC3174AB891DE1C62DB4D8F43EC3A@GIEXCHANGE.gidocs.net> We use this stainer, I would assess it as a good purchase. It is able to accept many racks at once; easy to set up, and the tech-rite support is great. It also has a stat start, and the stainer is able to prioritize your racks for you. The racks also never come across the load and unload stations; I like this feature. There is also more pots than a lot of the stainers. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, September 24, 2008 6:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] comments/opinions on Shandon Gemini H & E stainer Hello everyone- I wanted to see if anyone was willing to share any opinions, experiences or assessments of the Shandon Gemini stainer? This instrument is being considered for purchase by my laboratory. It will be primarily used for routine H & E staining and some cytology staining. We may only have limited ability to demo, so I wanted to see if anyone had anything that they might be willing to share regarding the pros and cons of this instrument. I have not personally used this instrument or many of its design style- so any information would be extremely helpful! Thanks so much! Joelle Weaver HTL(ASCP) _________________________________________________________________ Want to do more with Windows Live? Learn "10 hidden secrets" from Jamie. http://windowslive.com/connect/post/jamiethomson.spaces.live.com-Blog-cns!550F681DAD532637!5295.entry?ocid=TXT_TAGLM_WL_domore_092008_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Sep 25 09:21:38 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Sep 25 09:21:46 2008 Subject: [Histonet] QHIC Qualification Exam In-Reply-To: <380-220089324235538984@earthlink.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A390B@IS-E2K3.grhs.net> When looking use QIHC, not QHIC. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn Gamble Sent: Wednesday, September 24, 2008 6:56 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] QHIC Qualification Exam During the the NSH meeting in Pittsburgh, I responded to a few questions/misunderstandings about the QHIC examination. I'd like to share my responses with the Histonet community: You do not have to be ASCP Certified to take the Qualification examination. Individuals who work in research laboratories are eligible to take the Qualification examination if they work in the U.S. or Canada and meet the education or certification and experience requirements as defined in the selected Route. Individuals who work outside of the U.S. or Canada may qualify if they work in a CAP, JCAHO, or AABB accredited laboratory and meet the education or certification and experience requirements as defined in the selected Route. You can find complete information on the Qualification at www.ascp.org Marilyn Gamble marilynng@earthlink.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Sep 25 10:44:43 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Sep 25 10:44:54 2008 Subject: AW: [Histonet] Gi Biopsies and the Tissue Tek VIP In-Reply-To: <57BE698966D5C54EAE8612E8941D768303CE1298@EXCHANGE3.huntingtonhospital.com> Message-ID: Thanks for the info. We use the sponges and I doubt, that a short protocol is compatible with the fully filled cassettes. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com] Gesendet: Donnerstag, 25. September 2008 16:17 An: gu.lang@gmx.at Betreff: RE: [Histonet] Gi Biopsies and the Tissue Tek VIP We do not use sponges - most are wrapped in filter paper. We process anywhere from 40-100 biopsy cassettes daily. Laurie -----Original Message----- From: Gudrun Lang [mailto:gu.lang@gmx.at] Sent: Wednesday, September 24, 2008 11:45 PM To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Gi Biopsies and the Tissue Tek VIP Laurie, do you use the biopsy-sponges with this protocol? And how many cassettes do you process at one time? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Laurie Colbert Gesendet: Mittwoch, 24. September 2008 22:52 An: Amy Johnson Cc: histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Gi Biopsies and the Tissue Tek VIP We have all stations set at 10 minutes, except for the last xylene and the last two paraffins - which are 15 minutes each. We actually have the two formalins at one hour and one and a half hours, in case someone mistakenly puts the big tissue on the bx processor - at least they will be well-fixed before we have to re-process them. We have plenty of time to have the formalin at longer times, but if your specimens are already fixed, you should be able to go with the 10 minutes. Laurie Colbert Huntington Hospital (626) 397-8620 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, September 24, 2008 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gi Biopsies and the Tissue Tek VIP Can anyone out there help me figure out what is the shortest amount of time you can process GI biopsies with a Tissue Tek VIP? Thanks Amy Johnson Associates in Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pat.Patterson <@t> propath.com Thu Sep 25 11:35:59 2008 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Thu Sep 25 11:36:06 2008 Subject: [Histonet] Position in Dallas Message-ID: <82C7248978CB50469FD6BA68EBBEFE675E9793@exchange.propathlab.com> ProPath(r) is a progressive, CAP accredited, high-volume pathology practice located in Dallas, Texas and we are looking for an experienced Histology Technician/technologist to work in our Immunohistochemistry lab. Excellent microtomy skills required, IHC knowledge and experience desired - but not a must - we will train you!!! The hours are 4pm to 12:30am M-F. Call either Pat Patterson, Supervisor (214-237-1700 x2027) or Angie Viancourt in HR 214-237-1613 with questions or visit our website www.propath.com From PMonfils <@t> Lifespan.org Thu Sep 25 13:26:01 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Sep 25 13:26:07 2008 Subject: [Histonet] which one first - antigen retrieval or endo peroxidase block? Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C5F@LSRIEXCH1.lsmaster.lifespan.org> When doing immunoperoxidase staining on a tissue that requires antigen retrieval, which do you do first - the antigen retrieval or the endogenous peroxidase blocking? Or does it matter? From gvdobbin <@t> ihis.org Thu Sep 25 14:12:15 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Sep 25 14:12:43 2008 Subject: [Histonet] which one first - antigen retrieval or endo peroxidase block? Message-ID: I'd say it doesn't matter which is 1st, except my inclination is always to do the quench step 1st because the tissues seem a little less delicate before retreival than after. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "And in the end it's not the years in your life that count. It's the life in your years." - Abraham Lincoln >>> "Monfils, Paul" 9/25/2008 3:26:01 PM >>> When doing immunoperoxidase staining on a tissue that requires antigen retrieval, which do you do first - the antigen retrieval or the endogenous peroxidase blocking? Or does it matter? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From Bonnie.Whitaker <@t> osumc.edu Thu Sep 25 14:38:31 2008 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Thu Sep 25 14:39:13 2008 Subject: [Histonet] which one first - antigen retrieval or endoperoxidase block? In-Reply-To: References: Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F6026267A@msxc06.OSUMC.EDU> Hi Everyone, I have always done the quench 1st if I do it manually. If it is automated, then unless you have a newer instrument with onboard retrieval, you don't have a choice but to quench after the retrieval step (and may not have a choice then depending upon the system and how "open" it is). Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 Bonnie.Whitaker@osumc.edu phone 614.293.5048 fax 614.293.7273 pager 614.346.5013 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Thursday, September 25, 2008 3:12 PM To: PMonfils@Lifespan.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] which one first - antigen retrieval or endoperoxidase block? I'd say it doesn't matter which is 1st, except my inclination is always to do the quench step 1st because the tissues seem a little less delicate before retreival than after. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "And in the end it's not the years in your life that count. It's the life in your years." - Abraham Lincoln >>> "Monfils, Paul" 9/25/2008 3:26:01 PM >>> When doing immunoperoxidase staining on a tissue that requires antigen retrieval, which do you do first - the antigen retrieval or the endogenous peroxidase blocking? Or does it matter? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bonnie.Whitaker <@t> osumc.edu Thu Sep 25 14:46:20 2008 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Thu Sep 25 14:46:34 2008 Subject: [Histonet] Job in Columbus, Ohio Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F6026267B@msxc06.OSUMC.EDU> Hi Everyone, We have a night shift opening at Ohio State University Medical Center for a senior level histotech. The ideal candidate will be HT(ASCP) or HTL(ASCP), be a motivated, self-starter, and enjoy the fast-paced environment in a large teaching hospital. OSUMC is a great place to be! Feel free to contact me with any questions regarding the position! Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 Bonnie.Whitaker@osumc.edu phone 614.293.5048 fax 614.293.7273 From NSEARCY <@t> swmail.sw.org Thu Sep 25 14:53:53 2008 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Thu Sep 25 14:54:03 2008 Subject: [Histonet] Open Positions Message-ID: <48DBA601020000EF0000595C@MERCURY.SW.ORG> Scott & White Hospital & Clinic located in Temple, Texas ( one hour north of Austin) has two openings in the histology laboratory. A Lead Histotechnologist, as well as a bench histology technician, is needed for a state of the art laboratory affiliated with Texas A&M University. Certification required. Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 temple, Texas 76508 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division END:VCARD From rjbuesa <@t> yahoo.com Thu Sep 25 14:56:36 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Sep 25 14:56:40 2008 Subject: [Histonet] which one first - antigen retrieval or endo peroxidase block? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835C5F@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <363395.8745.qm@web65701.mail.ac4.yahoo.com> Antigen retrieval always comes first. Ren? J. --- On Thu, 9/25/08, Monfils, Paul wrote: From: Monfils, Paul Subject: [Histonet] which one first - antigen retrieval or endo peroxidase block? To: histonet@lists.utsouthwestern.edu Date: Thursday, September 25, 2008, 2:26 PM When doing immunoperoxidase staining on a tissue that requires antigen retrieval, which do you do first - the antigen retrieval or the endogenous peroxidase blocking? Or does it matter? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anne.lewin <@t> bms.com Thu Sep 25 15:07:51 2008 From: anne.lewin <@t> bms.com (Anne C Lewin) Date: Thu Sep 25 15:07:57 2008 Subject: [Histonet] which one first - antigen retrieval or endo peroxidase block? In-Reply-To: <363395.8745.qm@web65701.mail.ac4.yahoo.com> References: <363395.8745.qm@web65701.mail.ac4.yahoo.com> Message-ID: <48DBEF97.10004@bms.com> It doesn't really matter which one comes first - when I am using phospho-antibodies the IHC works better when the peroxidase block is done _before_ antigen retrieval. I have done the peroxidase block after antigen retrieval but before primary antibody, and also sometimes have waited until just before/after the biotinylated secondary antibody. The bottom line is that the peroxidase block needs to be done at some point before using the horseradish peroxidase (HRP complex), so that any endogenous peroxidase can be quenched and not give you a lot of background (especially with any RBC in the tissue). Rene J Buesa wrote: >Antigen retrieval always comes first. >Ren? J. > >--- On Thu, 9/25/08, Monfils, Paul wrote: > >From: Monfils, Paul >Subject: [Histonet] which one first - antigen retrieval or endo peroxidase block? >To: histonet@lists.utsouthwestern.edu >Date: Thursday, September 25, 2008, 2:26 PM > >When doing immunoperoxidase staining on a tissue that requires antigen >retrieval, which do you do first - the antigen retrieval or the endogenous >peroxidase blocking? Or does it matter? >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From FidgenL <@t> shmc.org Thu Sep 25 16:44:24 2008 From: FidgenL <@t> shmc.org (Fidgen, Laura L.) Date: Thu Sep 25 16:44:33 2008 Subject: [Histonet] Need a journal atricle Message-ID: <6BB8BC4519AAB844B174FC739A679BBC02DA8DE0@IRMEXCH01.irm.inhs.org> Hello everyone, I was hoping that someone out there had access to the Applied Immunohistochemistry and Molecular Morphology journal. I need a copy of an atricle from 2007, volume 15, issue 2. Would anyone be able to help? Thank you. Laura Fidgen, HT(ASCP)cm Histology Supervisor Sacred Heart Medical Center 509-474-4418 From dermpathsy <@t> gmail.com Thu Sep 25 16:52:50 2008 From: dermpathsy <@t> gmail.com (Sate Hamza) Date: Thu Sep 25 16:52:59 2008 Subject: [Histonet] Need a journal atricle In-Reply-To: <6BB8BC4519AAB844B174FC739A679BBC02DA8DE0@IRMEXCH01.irm.inhs.org> References: <6BB8BC4519AAB844B174FC739A679BBC02DA8DE0@IRMEXCH01.irm.inhs.org> Message-ID: <8854ff80809251452g1f187c14jbc5144217fb5469e@mail.gmail.com> What's the title of the article? Sate On Thu, Sep 25, 2008 at 4:44 PM, Fidgen, Laura L. wrote: > Hello everyone, > > I was hoping that someone out there had access to the Applied > Immunohistochemistry and Molecular Morphology journal. I need a copy of an > atricle from 2007, volume 15, issue 2. Would anyone be able to help? Thank > you. > -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada From bakevictoria <@t> gmail.com Thu Sep 25 21:18:27 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Sep 25 21:18:31 2008 Subject: [Histonet] Immunoperoxidase Control Tissue In-Reply-To: References: Message-ID: <4f016b690809251918n126832d9y2580034ac655b82a@mail.gmail.com> Hi Jackie! American Master Tech is one company that has a lot of different controls. Also try individual companies such as Cell Marque, that market antibodies. In some cases they do also sell or have sources that can sell these slides. Hope things are going well by you! Take Care, Vikki On Thu, Sep 25, 2008 at 5:11 AM, wrote: > I am interested in finding sources that sell control tissue for > immunoperoxidase and special stains. Does anyone have any suggestions? > Thanks > > > > Jacquelyn L. Grewe BS, HT (ASCP) > Laboratory Supervisor, Anatomic Pathology > Riverside Methodist Hospital > 614-566-4562 or 614-566-5679 > _______________________________________________ > > FORTUNE "100 Best Companies to Work for 2008 " > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tmalloy77 <@t> hotmail.com Fri Sep 26 03:44:01 2008 From: tmalloy77 <@t> hotmail.com (TIMOTHY MALLOY) Date: Fri Sep 26 03:44:09 2008 Subject: [Histonet] FEDERAL GOVERNMENT LABS Message-ID: Does anybody know if the Veterans or Government labs are CAP, JCAHO, AABB, ASCP, are Accedited, and if you are a employee how do you keep up an ascp cert. with no in house educational credits. TM. HT ( ASCP ) A.A.S. tmalloy77@hotmail.com This is email communication may contain CONFIDENTIAL INFORMATION WHICH ALSO MAY BE LEGALLY PRIVILEGED and is intended only for the use of the intended recipients identified above. If you are not the intended recipient of this communication, you are hereby notified that any unauthorized review, use, dissemination, distribution, downloading, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by reply email, delete the communication and destroy all copies. From rfields <@t> gidocs.net Fri Sep 26 08:02:35 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Fri Sep 26 08:03:19 2008 Subject: [Histonet] FEDERAL GOVERNMENT LABS Message-ID: <07732CE52EC3174AB891DE1C62DB4D8F43EC45@GIEXCHANGE.gidocs.net> Join NSH, they have great events, and a live learning center on the website where you can get credit for events recorded if you cannot make them in person. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of TIMOTHY MALLOY Sent: Friday, September 26, 2008 3:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FEDERAL GOVERNMENT LABS Does anybody know if the Veterans or Government labs are CAP, JCAHO, AABB, ASCP, are Accedited, and if you are a employee how do you keep up an ascp cert. with no in house educational credits. TM. HT ( ASCP ) A.A.S. tmalloy77@hotmail.com This is email communication may contain CONFIDENTIAL INFORMATION WHICH ALSO MAY BE LEGALLY PRIVILEGED and is intended only for the use of the intended recipients identified above. If you are not the intended recipient of this communication, you are hereby notified that any unauthorized review, use, dissemination, distribution, downloading, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by reply email, delete the communication and destroy all copies._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Fri Sep 26 08:03:27 2008 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Sep 26 08:06:04 2008 Subject: [Histonet] FEDERAL GOVERNMENT LABS In-Reply-To: References: Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D29A21B8B@LRGHEXVS1.practice.lrgh.org> Unless its changed over the years, My Naval lab in Virgina was both JCAHO and CAP inspected. Of course I am going back 20 years. Tom Podawiltz, HT (ASCP) ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of TIMOTHY MALLOY [tmalloy77@hotmail.com] Sent: Friday, September 26, 2008 4:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FEDERAL GOVERNMENT LABS Does anybody know if the Veterans or Government labs are CAP, JCAHO, AABB, ASCP, are Accedited, and if you are a employee how do you keep up an ascp cert. with no in house educational credits. TM. HT ( ASCP ) A.A.S. tmalloy77@hotmail.com This is email communication may contain CONFIDENTIAL INFORMATION WHICH ALSO MAY BE LEGALLY PRIVILEGED and is intended only for the use of the intended recipients identified above. If you are not the intended recipient of this communication, you are hereby notified that any unauthorized review, use, dissemination, distribution, downloading, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by reply email, delete the communication and destroy all copies._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From ktuttle <@t> umm.edu Fri Sep 26 11:46:57 2008 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Fri Sep 26 11:47:19 2008 Subject: [Histonet] ever heard of these antibodies? References: <48DCD838.90CE.001A.3@umm.edu> Message-ID: <48DCD9C0.90CE.001A.3@umm.edu> For IHC: 1.Nephrin 2.m-TOR 3.caspases Have you ever used them on FFPE? did it work ? where did you but them? Thank you Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From anh2006 <@t> med.cornell.edu Fri Sep 26 12:00:14 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri Sep 26 12:00:28 2008 Subject: [Histonet] ever heard of these antibodies? In-Reply-To: <48DCD9C0.90CE.001A.3@umm.edu> References: <48DCD838.90CE.001A.3@umm.edu> <48DCD9C0.90CE.001A.3@umm.edu> Message-ID: Anti-cleaved caspase 3 works beautifully from Cell Signaling. www.cellsignal.com >For IHC: > >1.Nephrin >2.m-TOR >3.caspases > >Have you ever used them on FFPE? did it work ? where did you but them? > >Thank you > >Kimberly C. Tuttle HT (ASCP) >Pathology Biorepository and Research Core >University of Maryland >Room NBW58, UMMC >22 S. Greene St >Baltimore, MD 21201 >(410) 328-5524 >(410) 328-5508 fax > > >Kimberly C. Tuttle HT (ASCP) >Pathology Biorepository and Research Core >University of Maryland >Room NBW58, UMMC >22 S. Greene St >Baltimore, MD 21201 >(410) 328-5524 >(410) 328-5508 fax > > > > >This e-mail and any accompanying attachments may be privileged, >confidential, contain protected health information about an >identified patient or be otherwise protected from disclosure. State >and federal law protect the confidentiality of this information. If >the reader of this message is not the intended recipient; you are >prohibited from using, disclosing, reproducing or distributing this >information; you should immediately notify the sender by telephone >or e-mail and delete this e-mail. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From brian.chelack <@t> pds.usask.ca Fri Sep 26 13:09:16 2008 From: brian.chelack <@t> pds.usask.ca (Brian Chelack) Date: Fri Sep 26 13:09:29 2008 Subject: [Histonet] Re: which one first - antigen retrieval or endo peroxidase block? Message-ID: <39EA5042E28B4CAEA9DEFCCA02119490@usask.ca> Ahh but it can matter!! The antigen retrieved epitope (enzymatic retrieval in this instance) recognized by the clone 15C5 for Bovine Viral Diarrhea (BVD) is destroyed by methanol. Thus if you are using hydrogen peroxide in methanol to block your endogenous peroxidases after performing antigen retrieval on a positive BVD case you will obtain a very weak or even negative result. It appears that the formalin cross linking protects the epitope from the effects of the methanol. So run your assay both ways if you are concerned, trust your observations. I learned this the hard way back in 1989. If you would be a real seeker after truth, it is necessary that at least once in your life you doubt, as far as possible, all things. Rene Descartes (1596 - 1650) Brian Chelack Special Projects Manager Prairie Diagnostic Services Inc. 52 Campus Drive, Saskatoon, SK S7N 5B4 Phone (306) 966-7211 Fax (306) 966-7244 Email brian.chelack@pds.usask.ca Confidentiality Notice: The information contained in this message and/or attachments may contain confidential and/or privileged information that is intended for the persons or entities addressed above. Any disclosure, copying, retransmission or dissemination is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy the message. From Pat.Patterson <@t> propath.com Fri Sep 26 14:21:36 2008 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Fri Sep 26 14:21:40 2008 Subject: [Histonet] Positions Dallas Message-ID: <82C7248978CB50469FD6BA68EBBEFE675E9E3F@exchange.propathlab.com> Come join us at ProPath, a progressive, CAP accredited, high-volume pathology practice located in Dallas, Texas and we are looking the following: Histology Tech: HT or HTL (ASCP) registered or eligible. Experience preferred. You will perform embedding and microtomy of paraffin-embedded tissue, operation of automated stainer and cover slipper, equipment maintenance, and record retention. 1am - 9:30am M-F Histology Manager: You will be responsible for staffing, management, planning, coordination and evaluation of all technical and operational activities of the Histology Department. Requirements include HT/HTL (ASCP) certification, a minimum of 8 years histology experience plus a minimum of 5 years supervisory experience. BS degree in Biological/Physical Science preferred, Associates degree required. Quality Assurance Coordinator: You will be responsible for coordinating the Quality Improvement and Quality Assurance programs to ensure regulatory and internal procedural compliance and overseeing our constant CAP accreditation preparedness. We require a minimum if 5 years working in a health-related position with extensive knowledge of process, quality improvement and CAP requirements. Website: www.propath.com. If you have any questions, please call Angie Viancourt at 214-237-1613. From Wanda.Smith <@t> HCAhealthcare.com Fri Sep 26 15:02:01 2008 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Fri Sep 26 15:02:06 2008 Subject: [Histonet] FW: Different Duties for HT vs HTL Message-ID: <817C2761C5A1394180709AEEDB775B7E06963C02@NASEV03.hca.corpad.net> WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax > ______________________________________________ > From: Smith Wanda > Sent: Friday, September 26, 2008 1:48 PM > To: 'histonet-request@lists.utsouthwestern.edu' > Subject: Different Duties for HT vs HTL > > Happy Friday Everyone, > Does anyone have different levels or duties for histology technician > w/ certificate or associate degree and a HTL with a BS degree? > If yes, do you have different job descriptions with different pay > scales? If yes, would anyone be willing to email a copy of your > different job descriptions? > I'm trying to justify a higher level histotech doing IHC and all that > goes with IHC vs ht's who only want to do routine work. > Please advise and share. > Thanks, > Wanda > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > From haozhang1 <@t> gmail.com Fri Sep 26 17:14:50 2008 From: haozhang1 <@t> gmail.com (hao zhang) Date: Fri Sep 26 17:14:54 2008 Subject: [Histonet] IHC protocol for 5 year old Pap smear slides Message-ID: <65992c0c0809261514t43246dbevb8f4a5b0e5d3dda0@mail.gmail.com> Hi, everyone I want to do pan-keratin IHC for some five year old pap smear slides. After I remove the coverslip then I used following protocol for destaining (xylene 5 min, two time change; 3% acetic alcohol (95%) overnight; 70% Etoh for 5 min) and then I put it in PBS, blocking, Prmary Ab, Secondary Ab and DAB. Problem is that I got less or nothing staining for pan-keratin Ab (even I used different dilution of Ab). Does anyone know how to recover the antigen activity for these slides? Any suggest will be apprecaited.--hao From talulahgosh <@t> gmail.com Sat Sep 27 04:56:33 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Sat Sep 27 04:56:40 2008 Subject: [Histonet] Re: [molecularbio] Re: D.N.A---PROTEIN... In-Reply-To: <497884.25289.qm@web51002.mail.re2.yahoo.com> References: <4746a9a0-6265-43ff-ba3c-6827d2421cc1@a29g2000pra.googlegroups.com> <497884.25289.qm@web51002.mail.re2.yahoo.com> Message-ID: Did anyone else see this as a bizarre question? My discomfort arises from the idea that someone would ask this question without spelling the words "please" and ""thanks" to an email to this list, and without thinking about their question What I mean to say is, if you can't figure out the RNA/DNA dogma (from whatever class or book, etc), why would you use text messaging to ask the answer? Don't we have google, or actual people to ask this question? And if you don't get that dogma, or its general idea, from lectures, shouldn't you be going to a school/mentor offering to teach you how to find the answer to your question? I understand some students may not get the idea of a central dogma from their studies, but applying to histonet seems the wrong answer to their confusion. I feel a question like this is easily answered by mentors/tutors. Or that students aren't understanding the basic ideas of replication and translation. Maybe it's a product of semantics, maybe it's a product of our ever increasing knowledge of DNA/RNA/protein interactions. Swiftly becoming a product of the before-internet world, Emily -- the velocity of time turns her voice into sugar water http://www.youtube.com/watch?v=jNA6zzoObxg From lpwenk <@t> sbcglobal.net Sat Sep 27 10:28:56 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Sep 27 10:29:11 2008 Subject: [Histonet] HTL Flash Cards? In-Reply-To: <667c97ab0809241311w517e21d3i2a151f9ae342fc8e@mail.gmail.com> Message-ID: <000601c920b5$c23f5670$0202a8c0@HPPav2> I had never heard of them, so I looked them up on the website. Morrison Media www.mo-med.com They sell all kinds of flashcards and study guides for lots of tests. Under the E-H category, where the HT and HTL flashcards have a link, there are also links to Electrician exam, Paramedic exam, First Responder test, Funeral Service test, GED, GRE, GMAT - you get the idea. According to their general blurb, they have experts in the field writing the flashcards. Does anyone know any histotech involved in writing the flashcards? I'd love for them to talk about this. I think for some people, having flashcards would fit with their style of learning - give them soundbites of information on topics, instead of having them read entire books. Or use this as a supplement to studying. Or have them in their purse/pocket where they can pull out a card and study on the go, instead of a book. But I do have some concerns about the flashcards and the test taking information this company supplies. If there is someone out there who helped write the cards, or someone who has bought the cards to respond, that would be helpful. - They have topics divided into 2 tests (have to pay separate for them) - HT and HTL. But when I look at what's on the HT exam vs. what's on the HTL exam, I have some concerns. - HTL need to know chemical fixatives, HT are supposed to know chemical and physical fixation. - HTL need to know autolysis, HT are supposed to know autolysis and putrefaction - HTL need to know acid decalcification, HT are supposed to know decalcification and chelation - HT are supposed to know Immunofluorescence, Electron microscopy, Carbowax and Celloidin, which are not listed on the HTL topics (yet ASCP HTL exam would include these topics, but ASCP HT exam would not) - The only stains listed for HT are H&E, Mucopolysaccharides, Hyaluronidase, Gomori Trichrome. - HTL stains include Connective tissue, PTAH, Bacteria, Fungus, Gram, Auramine-Rhodamine, Exogenous pigments, Minerals. Yet on the ASCP HT exam, all these stains are also required for the HT exam. You get the idea. Also, under the "Histotechnology Exam Secrets Study Guide", they are saying that their histotechnology exam study guide will help people "beat the test taking game", and that their research in the HT and HTL exam offered by ASCP "reveals specific weaknesses that you can exploit". Basically, what followed were test taking tips - how to guess to your advantage, how to tell the difference between right answers and clever sounding traps, how random bits of information often give away the right answer, how to look for key words to identify the correct answer, etc. Yet I know that the ASCP Board of Registry has (or at least did have) a psychometrician on staff - someone with a PhD in test writing, who works with the histotechs and pathologists writing the exam questions, to eliminate all the above "clues". Between this company's "tips" and the customer testimonials that they only studied for 1 week (one case, 5 hours) and passed the exam - I'm worried about people who aren't studying for the HT/HTL exam, and think these test taking clues will help them pass. This isn't like taking the GRE, where you can get by with some math and grammar background that can to be refreshed - the HT and HTL exams are based on a LOT of information that has to be LEARNED and APPLIED. The other concern I have is that the 3 flashcards they show as examples are still MEMORIZED information. What test takers have problems with are the PROBLEM-SOLVING and TROUBLESHOOTING aspects of the HT and HTL exams. Yes, they need to know what the oxidizer in the retic stain is, but they also need to know how to tell if it isn't working, or what to do if they run out, etc. So I'd love to hear from someone involved with writing these flashcards/study guides, and would love to hear from someone who actually bought them and used them. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kristen Yaros Sent: Wednesday, September 24, 2008 4:12 PM To: Histonet Subject: [Histonet] HTL Flash Cards? Has anyone heard of these? I just came across this site and was wondering if anyone has used these pre-made flash cards. http://www.flashcardsecrets.com/histotech/ -- Kristen Yaros, HT (ASCP)CM Histotechnology Society of Delaware Correspondence Secretary histotechkb@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat Sep 27 10:40:38 2008 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat Sep 27 10:40:44 2008 Subject: [Histonet] HTL Flash Cards? In-Reply-To: <000601c920b5$c23f5670$0202a8c0@HPPav2> References: <667c97ab0809241311w517e21d3i2a151f9ae342fc8e@mail.gmail.com> <000601c920b5$c23f5670$0202a8c0@HPPav2> Message-ID: I was not involved in writing any questions/answers. However I am thinking of buying some of the cards just of out curiousity! I can understand the concern about the memorization aspect versus true understanding, application- a.k.a "knowledge". I definately think that this would be a drawback. I have worked with a number of folks who seemed to have memorized information for the test but either do not seem to have retained any of it, and/or unable to apply any of the details of that information when in a real laboratory situation. (and have apparantly also lost the ability to look up needed information). I can also see that the HT versus HTL topic lists do not seem to coincide much with the topic and study lists published for registry exam preparation on the ASCP website? I know that the special stains list is most definately not a match. Go figure? It will be interesting to see what reply is posted. Joelle> From: lpwenk@sbcglobal.net> To: histotechkb@gmail.com; > Date: Sat, 27 Sep 2008 11:28:56 -0400> Subject: RE: [Histonet] HTL Flash Cards?> CC: > > I had never heard of them, so I looked them up on the website. Morrison> Media www.mo-med.com> > They sell all kinds of flashcards and study guides for lots of tests. Under> the E-H category, where the HT and HTL flashcards have a link, there are> also links to Electrician exam, Paramedic exam, First Responder test,> Funeral Service test, GED, GRE, GMAT - you get the idea. > > According to their general blurb, they have experts in the field writing the> flashcards. Does anyone know any histotech involved in writing the> flashcards? I'd love for them to talk about this.> > I think for some people, having flashcards would fit with their style of> learning - give them soundbites of information on topics, instead of having> them read entire books. Or use this as a supplement to studying. Or have> them in their purse/pocket where they can pull out a card and study on the> go, instead of a book.> > But I do have some concerns about the flashcards and the test taking> information this company supplies. If there is someone out there who helped> write the cards, or someone who has bought the cards to respond, that would> be helpful.> - They have topics divided into 2 tests (have to pay separate for them) - HT> and HTL. But when I look at what's on the HT exam vs. what's on the HTL> exam, I have some concerns.> - HTL need to know chemical fixatives, HT are supposed to know chemical and> physical fixation.> - HTL need to know autolysis, HT are supposed to know autolysis and> putrefaction> - HTL need to know acid decalcification, HT are supposed to know> decalcification and chelation> - HT are supposed to know Immunofluorescence, Electron microscopy, Carbowax> and Celloidin, which are not listed on the HTL topics (yet ASCP HTL exam> would include these topics, but ASCP HT exam would not)> - The only stains listed for HT are H&E, Mucopolysaccharides, Hyaluronidase,> Gomori Trichrome. > - HTL stains include Connective tissue, PTAH, Bacteria, Fungus, Gram,> Auramine-Rhodamine, Exogenous pigments, Minerals. Yet on the ASCP HT exam,> all these stains are also required for the HT exam.> You get the idea.> > Also, under the "Histotechnology Exam Secrets Study Guide", they are saying> that their histotechnology exam study guide will help people "beat the test> taking game", and that their research in the HT and HTL exam offered by ASCP> "reveals specific weaknesses that you can exploit".> > Basically, what followed were test taking tips - how to guess to your> advantage, how to tell the difference between right answers and clever> sounding traps, how random bits of information often give away the right> answer, how to look for key words to identify the correct answer, etc.> > Yet I know that the ASCP Board of Registry has (or at least did have) a> psychometrician on staff - someone with a PhD in test writing, who works> with the histotechs and pathologists writing the exam questions, to> eliminate all the above "clues".> > Between this company's "tips" and the customer testimonials that they only> studied for 1 week (one case, 5 hours) and passed the exam - I'm worried> about people who aren't studying for the HT/HTL exam, and think these test> taking clues will help them pass. This isn't like taking the GRE, where you> can get by with some math and grammar background that can to be refreshed -> the HT and HTL exams are based on a LOT of information that has to be> LEARNED and APPLIED.> > The other concern I have is that the 3 flashcards they show as examples are> still MEMORIZED information. What test takers have problems with are the> PROBLEM-SOLVING and TROUBLESHOOTING aspects of the HT and HTL exams. Yes,> they need to know what the oxidizer in the retic stain is, but they also> need to know how to tell if it isn't working, or what to do if they run out,> etc.> > So I'd love to hear from someone involved with writing these> flashcards/study guides, and would love to hear from someone who actually> bought them and used them.> > Peggy A. Wenk, HTL(ASCP)SLS> Beaumont Hospital> Royal Oak, MI 48073> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kristen> Yaros> Sent: Wednesday, September 24, 2008 4:12 PM> To: Histonet> Subject: [Histonet] HTL Flash Cards?> > Has anyone heard of these? I just came across this site and was wondering if> anyone has used these pre-made flash cards.> > http://www.flashcardsecrets.com/histotech/> > --> Kristen Yaros, HT (ASCP)CM> Histotechnology Society of Delaware> Correspondence Secretary> histotechkb@gmail.com> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Stay up to date on your PC, the Web, and your mobile phone with Windows Live. http://clk.atdmt.com/MRT/go/msnnkwxp1020093185mrt/direct/01/ From llewllew <@t> shaw.ca Sat Sep 27 12:10:03 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Sat Sep 27 12:10:08 2008 Subject: [Histonet] HTL Flash Cards? References: <000601c920b5$c23f5670$0202a8c0@HPPav2> Message-ID: <72AC8C9A850E4C81B7259993F3C0417B@Compaq> In the three example cards they show there are errors. In the first one it gives "liter" and "meter". I know this spelling is common in the United States, but I believe the SI standard is "litre" and "metre". In other words, include both. In the middle one, about reticulin staining, I would dispute the dogmatic nature of the information. Although this is a common explanation, it is not the modern one, which uses analogies to the photographic process. It is presented as absolute fact when it is an unproven suggestion as to what may happen. The final one, about dye structure, uses "chromogen" as a synonym for "chromophore", whereas it is a little used term for the dye+chromophore combination. For that reason, the final use of "chromophore" should actually be "chromogen". The word "auxophore" does not exist. It should be auxochrome. If you are going to use flash cards for improving rote learning of facts, make sure the facts being learned are correct. I suggest that students should have the cards checked out by an experienced educator technologist before using them, as first-learned information stays with you for decades. Bryan Llewellyn ----- Original Message ----- From: "Lee & Peggy Wenk" To: "'Kristen Yaros'" ; "'Histonet'" Sent: Saturday, September 27, 2008 8:28 AM Subject: RE: [Histonet] HTL Flash Cards? >I had never heard of them, so I looked them up on the website. Morrison > Media www.mo-med.com > > They sell all kinds of flashcards and study guides for lots of tests. > Under > the E-H category, where the HT and HTL flashcards have a link, there are > also links to Electrician exam, Paramedic exam, First Responder test, > Funeral Service test, GED, GRE, GMAT - you get the idea. > > According to their general blurb, they have experts in the field writing > the > flashcards. Does anyone know any histotech involved in writing the > flashcards? I'd love for them to talk about this. > > I think for some people, having flashcards would fit with their style of > learning - give them soundbites of information on topics, instead of > having > them read entire books. Or use this as a supplement to studying. Or have > them in their purse/pocket where they can pull out a card and study on the > go, instead of a book. > > But I do have some concerns about the flashcards and the test taking > information this company supplies. If there is someone out there who > helped > write the cards, or someone who has bought the cards to respond, that > would > be helpful. > - They have topics divided into 2 tests (have to pay separate for them) - > HT > and HTL. But when I look at what's on the HT exam vs. what's on the HTL > exam, I have some concerns. > - HTL need to know chemical fixatives, HT are supposed to know chemical > and > physical fixation. > - HTL need to know autolysis, HT are supposed to know autolysis and > putrefaction > - HTL need to know acid decalcification, HT are supposed to know > decalcification and chelation > - HT are supposed to know Immunofluorescence, Electron microscopy, > Carbowax > and Celloidin, which are not listed on the HTL topics (yet ASCP HTL exam > would include these topics, but ASCP HT exam would not) > - The only stains listed for HT are H&E, Mucopolysaccharides, > Hyaluronidase, > Gomori Trichrome. > - HTL stains include Connective tissue, PTAH, Bacteria, Fungus, Gram, > Auramine-Rhodamine, Exogenous pigments, Minerals. Yet on the ASCP HT exam, > all these stains are also required for the HT exam. > You get the idea. > > Also, under the "Histotechnology Exam Secrets Study Guide", they are > saying > that their histotechnology exam study guide will help people "beat the > test > taking game", and that their research in the HT and HTL exam offered by > ASCP > "reveals specific weaknesses that you can exploit". > > Basically, what followed were test taking tips - how to guess to your > advantage, how to tell the difference between right answers and clever > sounding traps, how random bits of information often give away the right > answer, how to look for key words to identify the correct answer, etc. > > Yet I know that the ASCP Board of Registry has (or at least did have) a > psychometrician on staff - someone with a PhD in test writing, who works > with the histotechs and pathologists writing the exam questions, to > eliminate all the above "clues". > > Between this company's "tips" and the customer testimonials that they only > studied for 1 week (one case, 5 hours) and passed the exam - I'm worried > about people who aren't studying for the HT/HTL exam, and think these test > taking clues will help them pass. This isn't like taking the GRE, where > you > can get by with some math and grammar background that can to be > refreshed - > the HT and HTL exams are based on a LOT of information that has to be > LEARNED and APPLIED. > > The other concern I have is that the 3 flashcards they show as examples > are > still MEMORIZED information. What test takers have problems with are the > PROBLEM-SOLVING and TROUBLESHOOTING aspects of the HT and HTL exams. Yes, > they need to know what the oxidizer in the retic stain is, but they also > need to know how to tell if it isn't working, or what to do if they run > out, > etc. > > So I'd love to hear from someone involved with writing these > flashcards/study guides, and would love to hear from someone who actually > bought them and used them. > > Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Hospital > Royal Oak, MI 48073 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kristen > Yaros > Sent: Wednesday, September 24, 2008 4:12 PM > To: Histonet > Subject: [Histonet] HTL Flash Cards? > > Has anyone heard of these? I just came across this site and was wondering > if > anyone has used these pre-made flash cards. > > http://www.flashcardsecrets.com/histotech/ > > -- > Kristen Yaros, HT (ASCP)CM > Histotechnology Society of Delaware > Correspondence Secretary > histotechkb@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmalloy77 <@t> hotmail.com Sun Sep 28 06:02:36 2008 From: tmalloy77 <@t> hotmail.com (TIMOTHY MALLOY) Date: Sun Sep 28 06:02:41 2008 Subject: [Histonet] HT ( QIHC QUESTION) Message-ID: HELLO ALL, What happens to a HT that has IHC on hands experience. Can and will they be eligible to sit for NSH Quailifing exam any ASCP examinationfor QIHC ???? TGM, HT ( ASCP ) A.A.S. tmalloy77@hotmail.com This email communication may contain CONFIDENTIAL INFORMATION WHICH ALSO MAY BE LEGALLY PRIVILEGED and is intended only for the use of the intended recipients identified above. If you are not the intended recipient of this communication, you are hereby notified that any unauthorized review, use, dissemination, distribution, downloading, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by reply email, delete the communication and destroy all copies. From lpwenk <@t> sbcglobal.net Sun Sep 28 12:55:58 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Sep 28 12:56:04 2008 Subject: [Histonet] HT ( QIHC QUESTION) In-Reply-To: Message-ID: <000601c92193$77134330$0202a8c0@HPPav2> ASCP is the organization that has the QIHC exam, not NSH. The people who write the exam questions are pathologists and histotechs (who are ASCP and NSH members). For information about the eligibility routes, go to: http://www.ascp.org/FunctionalNavigation/certification/QualificationinImmuno histochemistryQIHC.aspx It looks like you might qualify under route 2, if you also meet the experience requirements: Route 2: ASCP technician certification (HT, MLT) and twelve months full time acceptable experience in immunohistochemistry in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last five years Applicants must have experience in the following areas: Immunohistochemical and/or Immunofluorescence Preparations - All of the following should have been performed by the applicant: - Staining technique - Selection of proper control material - Titration of immunologic reagents - Quality Assurance The applicant should have participated in Quality Assurance related to all of the following: - Specimen fixation, processing, microtomy - Reagent selection, preparation, storage, disposal - Method selection, validation, documentation - Quality control - Safety Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of TIMOTHY MALLOY Sent: Sunday, September 28, 2008 7:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT ( QIHC QUESTION) HELLO ALL, What happens to a HT that has IHC on hands experience. Can and will they be eligible to sit for NSH Quailifing exam any ASCP examinationfor QIHC ???? TGM, HT ( ASCP ) A.A.S. tmalloy77@hotmail.com This email communication may contain CONFIDENTIAL INFORMATION WHICH ALSO MAY BE LEGALLY PRIVILEGED and is intended only for the use of the intended recipients identified above. If you are not the intended recipient of this communication, you are hereby notified that any unauthorized review, use, dissemination, distribution, downloading, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by reply email, delete the communication and destroy all copies._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sun Sep 28 18:22:27 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Sep 28 18:22:32 2008 Subject: [Histonet] Need volunteers for lab study Message-ID: <000e01c921c1$12a5cbb0$0202a8c0@HPPav2> I'm working on a research project for my master's class on safety management. It's involving brachytherapy seeds that are sometimes found in TURPS and whole prostates received in histology (grossing and sectioning). I'm gathering data as to how radioactive these seeds are X number of months after implantation. I'm finding out that most Radiation Safety Officers in most hospitals don't know that histology receives prostates with these seeds in them. Nor does Brachytherapy - they're assuming that once the seeds are implanted, they are in the patient for life. And no one knows if the seeds are still radioactive when we receive them in grossing and during sectioning. And I can't find any articles on the topics, expect that the NRC (National Regulatory Commission) says if the lab receives radioactive specimens, then we are under the same guidelines for radiation as, say, brachytherapy, nuclear medicine, etc. What I need are some other hospitals in the US to help me gather information as to number of times these seeds are received. For this class, I just need help gathering information in October and November. Please email me directly - If you are willing to: - let me know when you receive one/some of these seeds in a whole prostate or TURP - how many total number of whole prostates and TURPS you received in Oct and Nov (I'm hoping a quick computer search would work) - and if you are willing to do a little more checking when you receive any seeds, such as - patient history as to when the seeds were implanted (pathologist hopefully will get this history), and - if possible, if they are iodine (I-125) or palladium (Pd-103) (again, maybe the pathologist will find this on the patient's history). I do NOT need any information about the patient, so don't worry about HIPPA. I know I won't get that many cases at our hospital over the next two months (research paper due in December), so I'm hoping for more hospitals will participate, so I get better data. I'm particularly interested in hospitals that HAVE received seeds in the past, as I'm guessing these labs are more likely to receive the seeds in the future. Thanks in advance. Peggy Wenk Lpwenk@sbcglobal.net From sjchtascp <@t> yahoo.com Sun Sep 28 18:53:35 2008 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Sun Sep 28 18:53:38 2008 Subject: [Histonet] HT Madison, WI Message-ID: <839161.39229.qm@web38202.mail.mud.yahoo.com> I heard through the grapevine that an HT was hired at a local facility in Madison.? Since I'm looking for employment in this area I was hopiing someone might know if an HT?vacancy in the Madison area was created. ? Thanks, ? Steven Coakley 608-879-9556 From khbarr <@t> mdanderson.org Mon Sep 29 08:04:12 2008 From: khbarr <@t> mdanderson.org (Barr,Kaye H) Date: Mon Sep 29 08:04:47 2008 Subject: [Histonet] Positions Available Message-ID: The University of Texas M. D. Anderson Cancer Center in Houston, Texas has two Histology Technician positions available in the Frozen Section Laboratory. To apply go to www.mdanderson.org or call Dianna Menard @ 713-745-6184. Kaye Barr HT (ASCP) Laboratory Manager - Histology Department Division of Pathology & Laboratory Medicine 713-792-5366 khbarr@mdanderson.org From meghan.tucker <@t> yahoo.com Mon Sep 29 09:01:41 2008 From: meghan.tucker <@t> yahoo.com (Meghan Tucker) Date: Mon Sep 29 09:01:43 2008 Subject: [Histonet] Plus Slides Message-ID: <713645.28319.qm@web54501.mail.re2.yahoo.com> Good Morning! ? I was wondering where labs purchase their Plus slides from and also if anyone is having trouble with debris on their slides?? We have been having a lot of trouble with dirty slides right out of the box. ? Thanks! ? Meghan Tucker ? From pmarcum <@t> vet.upenn.edu Mon Sep 29 09:17:08 2008 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Mon Sep 29 09:17:11 2008 Subject: [Histonet] Plus Slides In-Reply-To: <713645.28319.qm@web54501.mail.re2.yahoo.com> References: <713645.28319.qm@web54501.mail.re2.yahoo.com> Message-ID: <000301c9223e$0efeb4e0$095a5b82@vet.upenn.edu> Hi, I would contact the vendor as they are usually resellers of the slides not the makers and need to know it there is a problem. We use Thermo Colorfrost and have good luck. Occasionally we do have a few bad slides as you will find with all vendors. I always call them and we get it fixes with out resorting to HistoNet. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Meghan Tucker Sent: Monday, September 29, 2008 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plus Slides Good Morning! ? I was wondering where labs purchase their Plus slides from and also if anyone is having trouble with debris on their slides?? We have been having a lot of trouble with dirty slides right out of the box. ? Thanks! ? Meghan Tucker ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fudo <@t> ufl.edu Mon Sep 29 09:35:20 2008 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Mon Sep 29 09:35:27 2008 Subject: [Histonet] blue paraffin Message-ID: <1741598343.101701222698920739.JavaMail.osg@osgjas01.cns.ufl.edu> Hi, all Does anyone know any vendors sell a blue paraffin? I've heard it will help to find some small tissues? Thanks, Ann From JWeems <@t> sjha.org Mon Sep 29 09:36:33 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Sep 29 09:36:37 2008 Subject: [Histonet] blue paraffin In-Reply-To: <1741598343.101701222698920739.JavaMail.osg@osgjas01.cns.ufl.edu> References: <1741598343.101701222698920739.JavaMail.osg@osgjas01.cns.ufl.edu> Message-ID: <982A0A9461F9BF438C7B19A6E425A3836A5A69@ITSSSXM01V6.one.ads.che.org> Surgipath does. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of FU,DONGTAO Sent: Monday, September 29, 2008 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] blue paraffin Hi, all Does anyone know any vendors sell a blue paraffin? I've heard it will help to find some small tissues? Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From rfields <@t> gidocs.net Mon Sep 29 09:39:23 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Mon Sep 29 09:40:10 2008 Subject: [Histonet] blue paraffin Message-ID: <07732CE52EC3174AB891DE1C62DB4D8F43EC4E@GIEXCHANGE.gidocs.net> We get ours from Surgipath. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of FU,DONGTAO Sent: Monday, September 29, 2008 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] blue paraffin Hi, all Does anyone know any vendors sell a blue paraffin? I've heard it will help to find some small tissues? Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Mon Sep 29 09:45:36 2008 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Sep 29 09:46:16 2008 Subject: [Histonet] Plus Slides In-Reply-To: <713645.28319.qm@web54501.mail.re2.yahoo.com> References: <713645.28319.qm@web54501.mail.re2.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D29A21B8F@LRGHEXVS1.practice.lrgh.org> We buy our slides from Mercdes, usually do not have a problem with them Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Meghan Tucker [meghan.tucker@yahoo.com] Sent: Monday, September 29, 2008 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plus Slides Good Morning! I was wondering where labs purchase their Plus slides from and also if anyone is having trouble with debris on their slides? We have been having a lot of trouble with dirty slides right out of the box. Thanks! Meghan Tucker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From vazquezr <@t> ohsu.edu Mon Sep 29 09:48:07 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon Sep 29 09:48:26 2008 Subject: [Histonet] blue paraffin In-Reply-To: <1741598343.101701222698920739.JavaMail.osg@osgjas01.cns.ufl.edu> References: <1741598343.101701222698920739.JavaMail.osg@osgjas01.cns.ufl.edu> Message-ID: <2A582E8156B45F468A62D1F1D20AF08338CEF1@EX-BE08.ohsu.edu> Hello, Maybe try some blue food coloring and stir it up. Never tried it though. Robyn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of FU,DONGTAO Sent: Monday, September 29, 2008 7:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] blue paraffin Hi, all Does anyone know any vendors sell a blue paraffin? I've heard it will help to find some small tissues? Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wecare <@t> qualityhistology.com Mon Sep 29 09:59:40 2008 From: wecare <@t> qualityhistology.com (wecare@qualityhistology.com) Date: Mon Sep 29 09:59:49 2008 Subject: [Histonet] blue paraffin References: <1741598343.101701222698920739.JavaMail.osg@osgjas01.cns.ufl.edu> <2A582E8156B45F468A62D1F1D20AF08338CEF1@EX-BE08.ohsu.edu> Message-ID: At arts and craft stores (Michael's, Ragshop) that sell supply for candle making, they also have special coloring agent for paraffin/wax. ----- Original Message ----- From: "Robyn Vazquez" To: "FU,DONGTAO" ; Sent: Monday, September 29, 2008 10:48 AM Subject: RE: [Histonet] blue paraffin Hello, Maybe try some blue food coloring and stir it up. Never tried it though. Robyn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of FU,DONGTAO Sent: Monday, September 29, 2008 7:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] blue paraffin Hi, all Does anyone know any vendors sell a blue paraffin? I've heard it will help to find some small tissues? Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kenneth.metzger <@t> aruplab.com Mon Sep 29 10:15:28 2008 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Mon Sep 29 10:15:36 2008 Subject: [Histonet] Block "shippers" Message-ID: Recently we have had paraffin blocks shipped to our facility in clear, four chambered carriers. Can anyone tell me where I can get these? Thanks, Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metzger@aruplab.com (801) 583-2787 x 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From pruegg <@t> ihctech.net Mon Sep 29 10:47:18 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Sep 29 10:47:25 2008 Subject: [Histonet] Plus Slides In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638631D29A21B8F@LRGHEXVS1.practice.lrgh.org> References: <713645.28319.qm@web54501.mail.re2.yahoo.com> <38667E7FB77ECD4E91BFAEB8D98638631D29A21B8F@LRGHEXVS1.practice.lrgh.org> Message-ID: I also use slides from Mercedes, don't see dirt debris but I do see that the slides take up the hematoxylin stain, it does not seem to cause a problem though. Mercedes sells the cheapest charged slides I can find anywhere. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Monday, September 29, 2008 8:46 AM To: meghan.tucker@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Plus Slides We buy our slides from Mercdes, usually do not have a problem with them Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Meghan Tucker [meghan.tucker@yahoo.com] Sent: Monday, September 29, 2008 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plus Slides Good Morning! I was wondering where labs purchase their Plus slides from and also if anyone is having trouble with debris on their slides? We have been having a lot of trouble with dirty slides right out of the box. Thanks! Meghan Tucker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sharon.Genest <@t> saskatoonhealthregion.ca Mon Sep 29 11:38:35 2008 From: Sharon.Genest <@t> saskatoonhealthregion.ca (Genest, Sharon SktnHR) Date: Mon Sep 29 11:40:58 2008 Subject: [Histonet] amoebae Message-ID: Has anyone ever used PAS Mentanil yellow for staining amoebae. Sharon Genest MLT Technologist Supervisor Histology Saskatoon Health Region Phone: (306)655-8197 Email: sharon.genest@saskatoonhealthregion.ca From Saro.Bascaramurty <@t> nrc-cnrc.gc.ca Mon Sep 29 11:53:39 2008 From: Saro.Bascaramurty <@t> nrc-cnrc.gc.ca (Bascaramurty, Saro) Date: Mon Sep 29 11:54:34 2008 Subject: [Histonet] Freezing tissues Message-ID: <00E9EC516E0CEB4A9ED53E7FAD19DE090235493A@nrccenexb1.nrc.ca> Hello, Does anyone out there have a good method to freeze animal vessels in order to have good morphology/free of artefacts etc. for some spectroscopic studies and histochemistry? Using liquid nitrogen is ok, but not isopentane or any other chemicals that would interfere with the spectra. Thanks in advance for any input that would improve our freezing method. Saro From jennifer.l.hofecker <@t> Vanderbilt.Edu Mon Sep 29 12:56:24 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Mon Sep 29 12:56:31 2008 Subject: [Histonet] Plus Slides In-Reply-To: References: <713645.28319.qm@web54501.mail.re2.yahoo.com><38667E7FB77ECD4E91BFAEB8D98638631D29A21B8F@LRGHEXVS1.practice.lrgh.org> Message-ID: <898D946569A27444B65667A49C07405201DFE459@mailbe06.mc.vanderbilt.edu> Me too! Me too! I also buy my plus slides from Mercedes. They sell a few different types. I buy the Starfrost Platinum line. Not only are they affordable, they are very high quality slides. I don't usually even have a problem with dye uptake. Call them and ask for a sample. Have a great week. Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Monday, September 29, 2008 10:47 AM To: 'Podawiltz, Thomas'; meghan.tucker@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Plus Slides I also use slides from Mercedes, don't see dirt debris but I do see that the slides take up the hematoxylin stain, it does not seem to cause a problem though. Mercedes sells the cheapest charged slides I can find anywhere. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Monday, September 29, 2008 8:46 AM To: meghan.tucker@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Plus Slides We buy our slides from Mercdes, usually do not have a problem with them Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Meghan Tucker [meghan.tucker@yahoo.com] Sent: Monday, September 29, 2008 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plus Slides Good Morning! I was wondering where labs purchase their Plus slides from and also if anyone is having trouble with debris on their slides? We have been having a lot of trouble with dirty slides right out of the box. Thanks! Meghan Tucker _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SmithSR <@t> crstlukes.com Mon Sep 29 12:58:29 2008 From: SmithSR <@t> crstlukes.com (Smith, Susan R.) Date: Mon Sep 29 12:58:38 2008 Subject: [Histonet] Rapid tissue processors Message-ID: We are in the process of looking for a rapid tissue processor. The one's we are looking at are: Leica Peloris Rapid Tissue Processor Tissue-Tek Xpress x50 (Sakura) STP 420D Tissue Processor (Microm from Richard-Allan Scientific)/Thermo Fisher Scientific RHS-1/RHS-2 Rapid Microwave Histoprocessor for specimens up to 5mm thickness (Milestone) PATHOS automatic rapid microwave histoprocessor for specimens up to 5mm thickness (Milestone) I have seen some comments on the Peloris and Tissue-Tek express, but nothing on the others. Please give me your in-put. Thanks, Sue Smith, MT(ASCP)SM Laboratory Supervisor St. Luke's Hospital 1026 A Ave NE Cedar Rapids, IA 52402 ph: 319-369-8845 fax: 319-369-8095 e-mail: smithsr@crstlukes.com ******************************************** This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. ?? 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ********************************************* From vazquezr <@t> ohsu.edu Mon Sep 29 12:59:22 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon Sep 29 12:59:39 2008 Subject: [Histonet] Freezing tissues In-Reply-To: <00E9EC516E0CEB4A9ED53E7FAD19DE090235493A@nrccenexb1.nrc.ca> References: <00E9EC516E0CEB4A9ED53E7FAD19DE090235493A@nrccenexb1.nrc.ca> Message-ID: <2A582E8156B45F468A62D1F1D20AF08338CF3C@EX-BE08.ohsu.edu> Saro, Just a thought, could you use a clear water soluble microbiology gelatinous plate? Never done this, but it would keep them standing on edge. Robyn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bascaramurty, Saro Sent: Monday, September 29, 2008 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freezing tissues Hello, Does anyone out there have a good method to freeze animal vessels in order to have good morphology/free of artefacts etc. for some spectroscopic studies and histochemistry? Using liquid nitrogen is ok, but not isopentane or any other chemicals that would interfere with the spectra. Thanks in advance for any input that would improve our freezing method. Saro _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brod033 <@t> gmail.com Mon Sep 29 13:01:01 2008 From: brod033 <@t> gmail.com (Ben Spirto) Date: Mon Sep 29 13:01:04 2008 Subject: [Histonet] 4% Paraformaldehide Message-ID: <287d127c0809291101y230458f3j66128d4f7a22c370@mail.gmail.com> How can I measure the strength of 4 % paraformaldehide? It seems that our lab made 4% para does not adequately penetrate the brain tissue. Any suggestions? From contact <@t> excaliburpathology.com Mon Sep 29 13:16:09 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Sep 29 13:16:12 2008 Subject: [Histonet] 4% Paraformaldehide Message-ID: <825160.26878.qm@web1102.biz.mail.sk1.yahoo.com> Hello, ? What species and how long are you fixing? ? Paula ----- Original Message ---- From: Ben Spirto To: Histonet@lists.utsouthwestern.edu Sent: Monday, September 29, 2008 1:01:01 PM Subject: [Histonet] 4% Paraformaldehide How can I measure the strength of 4 % paraformaldehide? It seems that our lab made 4% para does not adequately penetrate the brain tissue. Any suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Mon Sep 29 14:35:46 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Sep 29 14:35:10 2008 Subject: [Histonet] 4% Paraformaldehide In-Reply-To: <287d127c0809291101y230458f3j66128d4f7a22c370@mail.gmail.com> References: <287d127c0809291101y230458f3j66128d4f7a22c370@mail.gmail.com> Message-ID: <48E12E12.5050801@umdnj.edu> Hi Ben: Do you mean rate of penetration, speed of fixation or the actual concentration of formaldehyde in the solution? Remember, formaldehyde penetrates tissue rapidly BUT fixes tissue slowly. Geoff Ben Spirto wrote: > How can I measure the strength of 4 % paraformaldehide? It seems that our > lab made 4% para does not adequately penetrate the brain tissue. Any > suggestions? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From jcline <@t> wchsys.org Mon Sep 29 14:37:09 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Mon Sep 29 14:37:13 2008 Subject: [Histonet] JOB OPENING Message-ID: I have an opening for a full time Histotechnican. M-F 6:00am to 4:30pm. We are a rural hospital located in Hagerstown, Md. The lab is part of the for-profit side of the organization. We do about 17,000 cases a year. We are one hour from Baltimore and Washington D.C. Call our H.R. department at 301-665-4500. Joyce Cline Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax301-665-4941 ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Sep 29 14:39:53 2008 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Sep 29 14:40:45 2008 Subject: [Histonet] SV40 antibody Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB0028AE83D@e2k3ms1.urmc-sh.rochester.edu> I need some help with getting sv40 to work in paraffin. Will someone share there source and protocol. Thanks in advance. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Sep 29 14:42:01 2008 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Sep 29 14:42:50 2008 Subject: [Histonet] sv40 antibody Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB0028AE83E@e2k3ms1.urmc-sh.rochester.edu> I need some help getting sv40 to work in paraffin sections. Will someone share a source and protocol. Thanks in advance. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 From jcline <@t> wchsys.org Mon Sep 29 15:40:24 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Mon Sep 29 15:40:27 2008 Subject: [Histonet] JOB OPENING In-Reply-To: Message-ID: <6BE07D121671442FA0415CA91E31B5D0@wchsys.org> Sorry, I should have said three shifts starting at 6:00am, 7:00am, and 7:30am. With a late Friday 9:30am - 6:00pm once a month. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Monday, September 29, 2008 3:37 PM To: Histonet Subject: [Histonet] JOB OPENING I have an opening for a full time Histotechnican. M-F 6:00am to 4:30pm. We are a rural hospital located in Hagerstown, Md. The lab is part of the for-profit side of the organization. We do about 17,000 cases a year. We are one hour from Baltimore and Washington D.C. Call our H.R. department at 301-665-4500. Joyce Cline Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax301-665-4941 ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From vazquezr <@t> ohsu.edu Mon Sep 29 15:44:27 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon Sep 29 15:44:42 2008 Subject: [Histonet] cryostat Message-ID: <2A582E8156B45F468A62D1F1D20AF08338CF7C@EX-BE08.ohsu.edu> Hello, We are looking for a good refurbished cryostat for a back up. Our back up has gone caput. Anyone know of any. E-Bay has some. Robyn From AGrobe2555 <@t> aol.com Mon Sep 29 15:59:47 2008 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Sep 29 15:59:53 2008 Subject: [Histonet] Re: Which one first - antigen retrieval or peroxidase block Message-ID: I'm with Rene on this one. Block after retrieval. For those of you who do the block before retrieval, how do you control for any activity that might be exposed during the retrieval step? Couldn't it lead to false positives in your slides? Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana Foundation **************Looking for simple solutions to your real-life financial challenges? Check out WalletPop for the latest news and information, tips and calculators. (http://www.walletpop.com/?NCID=emlcntuswall00000001) From jdooley2008 <@t> yahoo.com Mon Sep 29 18:12:39 2008 From: jdooley2008 <@t> yahoo.com (James Dooley) Date: Mon Sep 29 18:12:42 2008 Subject: [Histonet] paraffin sectioning problems Message-ID: <714726.14054.qm@web45908.mail.sp1.yahoo.com> I recently started paraffin sectioning and I am having trouble with getting good quality sections reproducibly. I was wondering if you have any tips that may help. I am sectioning thymus, pancreas, and lung. Section thickness is 10 um. The microtome that I am using is Reichert-Jung Supercut, disposable knife holder by Leica, and Accu-Edge low profile blades. I fix the tissue in a modified Carnoy's fix overnight, dehydrate in graded alcohol, 100% ETOH, Toluene 2x15 minutes, Paraffin at 65 degrees overnight with 2X changes of paraffin. Embed and store at RT. The paraffin I am using is Fisherbrand Paraplast X-tra Tissue embedding medium. Any suggestion would be greatly appreciated. Thank you in advance, James Dooley From gvdobbin <@t> ihis.org Mon Sep 29 21:12:55 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Mon Sep 29 21:13:19 2008 Subject: [Histonet] Re: Which one first - antigen retrieval or peroxidase block Message-ID: Peroxidase is an enzyme. It doesn't miraculously appear as a result to exposure to heat. You show me one one slide where this has occurred and I will humbly remain silent in this forum for a whole month! Ok, maybe just a week... :-) Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "And in the end it's not the years in your life that count. It's the life in your years." - Abraham Lincoln >>> 09/29/08 5:59 PM >>> I'm with Rene on this one. Block after retrieval. For those of you who do the block before retrieval, how do you control for any activity that might be exposed during the retrieval step? Couldn't it lead to false positives in your slides? Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana Foundation **************Looking for simple solutions to your real-life financial challenges? Check out WalletPop for the latest news and information, tips and calculators. (http://www.walletpop.com/?NCID=emlcntuswall00000001) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From holmberg.katie <@t> gmail.com Tue Sep 30 00:37:23 2008 From: holmberg.katie <@t> gmail.com (katie holmberg) Date: Tue Sep 30 00:37:28 2008 Subject: [Histonet] Openings Minneapolis Area Message-ID: Is anyone in need of or aware of Histotech positions in the Minneapolis area. Thanks! From rjbuesa <@t> yahoo.com Tue Sep 30 07:45:55 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 30 07:45:58 2008 Subject: [Histonet] paraffin sectioning problems In-Reply-To: <714726.14054.qm@web45908.mail.sp1.yahoo.com> Message-ID: <272123.98551.qm@web65712.mail.ac4.yahoo.com> It is likely that you have an infiltration problem and toluene twice for 15 minutes each is too little. Also your tissue subjects are regularly quite difficult. Go to any histotechniques manual and try to reproduce one of the methods in it. This would be a good starting point. Ren? J. --- On Mon, 9/29/08, James Dooley wrote: From: James Dooley Subject: [Histonet] paraffin sectioning problems To: histonet@lists.utsouthwestern.edu Date: Monday, September 29, 2008, 7:12 PM I recently started paraffin sectioning and I am having trouble with getting good quality sections reproducibly. I was wondering if you have any tips that may help. I am sectioning thymus, pancreas, and lung. Section thickness is 10 um. The microtome that I am using is Reichert-Jung Supercut, disposable knife holder by Leica, and Accu-Edge low profile blades. I fix the tissue in a modified Carnoy's fix overnight, dehydrate in graded alcohol, 100% ETOH, Toluene 2x15 minutes, Paraffin at 65 degrees overnight with 2X changes of paraffin. Embed and store at RT. The paraffin I am using is Fisherbrand Paraplast X-tra Tissue embedding medium. Any suggestion would be greatly appreciated. Thank you in advance, James Dooley _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Sep 30 07:51:12 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 30 07:51:20 2008 Subject: [Histonet] Re: Which one first - antigen retrieval or peroxidase block In-Reply-To: Message-ID: <940649.76029.qm@web65704.mail.ac4.yahoo.com> As far as I remember enzymes are proteins and are they not supposed to cross-link with formalin also? Ren? J. --- On Mon, 9/29/08, Greg Dobbin wrote: From: Greg Dobbin Subject: Re: [Histonet] Re: Which one first - antigen retrieval or peroxidase block To: AGrobe2555@aol.com, histonet@lists.utsouthwestern.edu Date: Monday, September 29, 2008, 10:12 PM Peroxidase is an enzyme. It doesn't miraculously appear as a result to exposure to heat. You show me one one slide where this has occurred and I will humbly remain silent in this forum for a whole month! Ok, maybe just a week... :-) Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "And in the end it's not the years in your life that count. It's the life in your years." - Abraham Lincoln >>> 09/29/08 5:59 PM >>> I'm with Rene on this one. Block after retrieval. For those of you who do the block before retrieval, how do you control for any activity that might be exposed during the retrieval step? Couldn't it lead to false positives in your slides? Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana Foundation **************Looking for simple solutions to your real-life financial challenges? Check out WalletPop for the latest news and information, tips and calculators. (http://www.walletpop.com/?NCID=emlcntuswall00000001) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Sep 30 08:05:14 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Sep 30 08:05:12 2008 Subject: [Histonet] Re: Which one first - antigen retrieval or peroxidaseblock In-Reply-To: <940649.76029.qm@web65704.mail.ac4.yahoo.com> Message-ID: Again this is all antigen dependent. CD4 is one that I have found does better if you do endogenous enzyme block after AR and for that one I also make sure I use h202 in methanol, so it may be the fixing action of the methanol as much as the blocking of the h202 at play here. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, September 30, 2008 6:51 AM To: AGrobe2555@aol.com; histonet@lists.utsouthwestern.edu; Greg Dobbin Subject: Re: [Histonet] Re: Which one first - antigen retrieval or peroxidaseblock As far as I remember enzymes are proteins and are they not supposed to cross-link with formalin also? Ren? J. --- On Mon, 9/29/08, Greg Dobbin wrote: From: Greg Dobbin Subject: Re: [Histonet] Re: Which one first - antigen retrieval or peroxidase block To: AGrobe2555@aol.com, histonet@lists.utsouthwestern.edu Date: Monday, September 29, 2008, 10:12 PM Peroxidase is an enzyme. It doesn't miraculously appear as a result to exposure to heat. You show me one one slide where this has occurred and I will humbly remain silent in this forum for a whole month! Ok, maybe just a week... :-) Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "And in the end it's not the years in your life that count. It's the life in your years." - Abraham Lincoln >>> 09/29/08 5:59 PM >>> I'm with Rene on this one. Block after retrieval. For those of you who do the block before retrieval, how do you control for any activity that might be exposed during the retrieval step? Couldn't it lead to false positives in your slides? Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana Foundation **************Looking for simple solutions to your real-life financial challenges? Check out WalletPop for the latest news and information, tips and calculators. (http://www.walletpop.com/?NCID=emlcntuswall00000001) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cormier <@t> MIT.EDU Tue Sep 30 08:05:50 2008 From: cormier <@t> MIT.EDU (Kathy Cormier) Date: Tue Sep 30 08:05:57 2008 Subject: [Histonet] ? ed clark Message-ID: <099946BB7DD0477A81A147C554F14608@mit.edu> Hi All, Looking to locate Ed Clark, used to be at Praecis.... Ed give me a quick call or email (cormier@mit.edu ) Thanks! Kathy DCM MIT From relia1 <@t> earthlink.net Tue Sep 30 08:52:13 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Sep 30 08:52:20 2008 Subject: [Histonet] RELIA Histology Careers Bulletin 9-30-08 Message-ID: Hello Histonetters! It is hard to believe the summer is over already. Before we know it, it will be 2009! Let?s Get The Ball Rolling!!! Looking for a new job in the Fall? Planning a job change after the holidays? Considering making a move in 2009? Are you a histo tech looking for a better opportunity? Are you a new or recent graduate of a histology school? Are you a traveler considering transitioning into a permanent position? Are you a Histo Tech looking for an opportunity to advance into management? Let?s Get The Ball Rolling!!! Do we need to freshen up your resume? I can help!! Do you need help with what your next career move should be? I can help!! Do you need assistance with salary negotiation? I can help!! Do you want someone to work with you on what to say in an interview? I can help!! How about coordinating times and dates for the interview? I can help!! All of the positions that I work with are full time permanent positions with premier companies setting the mark to be "employers of choice" in their respective areas. My clients offer competitive salaries excellent benefits and relocation assistance. If you or anyone you know is considering making a job change all it takes is a 5 minute phone call or a quick e-mail to Get The Ball Rolling!!! We can talk about my current openings and what you are looking for in your next position. Here are some of my current openings: HISTOLOGY MANAGEMENT: Manchester, NH ? Brand new Pathology Lab Buffalo NY ? Histology Manager for Renown Clinical and Research Facility Portland, OR ? Histology and Cytology Manager ? no bench work. Los Angeles, CA ? Afternoon shift for National Reference Lab El Paso, TX ? Lab Supervisor for hospital environment HISTOTECHS Washington DC ? Histotech Prestigious Hospital Environment PA- Pittsburgh, Dayshift HT or HTL required. TX - Austin, Pathology Lab Entry Level and experience techs needed TX - Dallas , Histotechnician/Histotechnologist TX ? El Paso, Histo tech WA ? Seattle Clinical Positions on Night Shift $$$ Shift diff!! WA ? Seattle IHC Tech WA - Seattle area, Research position WA - Spokane, Clinical Position in Brand new lab! MD - Baltimore, Grossing Histotechnologist CA - Los Angeles, Histotechnician/Histotechnologist ASCP cert req MA ? North of Boston, IHC Tech NY ? New York City Flow Cytometry Tech NY ? Uniondale Histotech Specialty Lab Environment OTHER POSITIONS FL ? Tampa Pathologist Assistant NY ? New York City, Flow Cytometry Tech WA ? Seattle, IHC Tech MA ? North of Boston IHC Tech Remember it never hurts to look! If you or someone you know is interested in any of these opportunities please let me know. Also please feel free to forward this e-mail to your friends and colleagues as well I can be reached toll free at 866-60-RELIA (866-607-3542) or relia1@earthlink.net Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From mohawkman71 <@t> yahoo.com Tue Sep 30 09:30:08 2008 From: mohawkman71 <@t> yahoo.com (Thomas Isbell) Date: Tue Sep 30 09:30:14 2008 Subject: [Histonet] looking for Sheehan Message-ID: <724801.8667.qm@web36108.mail.mud.yahoo.com> I am looking for a "Theory & Practice of Histotechnology" by Sheehan.? I would love to have one in good to great condition.? I will pay a fair price for the book.? The one at my local library was missing several pages from several chapters. ? Thanks, ? Tom aka mohawkman71 ? PS. From jqb7 <@t> cdc.gov Tue Sep 30 09:33:32 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Sep 30 09:34:03 2008 Subject: [Histonet] looking for Sheehan In-Reply-To: <724801.8667.qm@web36108.mail.mud.yahoo.com> References: <724801.8667.qm@web36108.mail.mud.yahoo.com> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C2AE@LTA3VS011.ees.hhs.gov> Amazon has new copies............. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Isbell Sent: Tuesday, September 30, 2008 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] looking for Sheehan I am looking for a "Theory & Practice of Histotechnology" by Sheehan.? I would love to have one in good to great condition.? I will pay a fair price for the book.? The one at my local library was missing several pages from several chapters. ? Thanks, ? Tom aka mohawkman71 ? PS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mroark <@t> sfmc.net Tue Sep 30 10:01:52 2008 From: mroark <@t> sfmc.net (Matt Roark) Date: Tue Sep 30 10:02:42 2008 Subject: [Histonet] Lot Logs References: <000601c92193$77134330$0202a8c0@HPPav2> Message-ID: <00f801c9230d$7b5784d0$06570181@sfmc.net> Does everyone keep logs of lot numbers for their xylene and alcohol bottles? I can see keeping track of lot numbers for stains and antibodies but I can not find in the CAP checklist where they would want us to keep track of the lots of xylene and or alcohol. What does everyone else do? Thanks!! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 From rjbuesa <@t> yahoo.com Tue Sep 30 10:12:41 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 30 10:12:46 2008 Subject: [Histonet] Lot Logs In-Reply-To: <00f801c9230d$7b5784d0$06570181@sfmc.net> Message-ID: <265230.1764.qm@web65714.mail.ac4.yahoo.com> I never did that BUT if you have a problem during any step of your process it is a good practice to consult the supplier with the lot number of their products to find out if the problem you experienced has been reported by others also. Ren? J. --- On Tue, 9/30/08, Matt Roark wrote: From: Matt Roark Subject: [Histonet] Lot Logs To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 30, 2008, 11:01 AM Does everyone keep logs of lot numbers for their xylene and alcohol bottles? I can see keeping track of lot numbers for stains and antibodies but I can not find in the CAP checklist where they would want us to keep track of the lots of xylene and or alcohol. What does everyone else do? Thanks!! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amartinez <@t> carisdx.com Tue Sep 30 10:12:43 2008 From: amartinez <@t> carisdx.com (Martinez, Angela) Date: Tue Sep 30 10:12:53 2008 Subject: [Histonet] Great opportunity for Histotechnician in brand new laboratory! Message-ID: <9B8A3AC772C7F64680392A7CB8FBFB0F05EC0582@s-irv-ex301.PathologyPartners.intranet> Great opportunity for Histotechnician in brand new laboratory! Gastrointestinal Associates of North Texas in Ft Worth , is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and meet CLIA-88 regulations to perform gross dissection. Prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. The position offers competitive salary, medical insurance, retirement plan, and vacation /sick leave. Interested applicants should e-mail resumes to Meredith Hale at mhale@carisdx.com . Meredith Hale HT (ASCP) Technical Director Caris Diagnostics 8400 Esters Blvd. Ste. 190 Irving, Texas 75063 469-648-8253 Cell 214-596-2219 Office 972-929-9996 Fax Angela Martinez Internal Recruiter Caris Diagnostics 8400 Esters Blvd. Suite 190 Irving, Texas 75063 (214) 277-8700 Phone (214) 596-7490Fax From ktuttle <@t> umm.edu Tue Sep 30 10:24:55 2008 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Tue Sep 30 10:25:16 2008 Subject: [Histonet] PLS Help with IHC protocol Message-ID: <48E20C86.90CE.001A.3@umm.edu> Step 10: Add antibody at 10ug/ml in PBS + 0.1%BSA + 1:10 volume CAS block Can someone break this down for me in simple instructions? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From pruegg <@t> ihctech.net Tue Sep 30 10:52:46 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Sep 30 10:52:55 2008 Subject: [Histonet] PLS Help with IHC protocol In-Reply-To: <48E20C86.90CE.001A.3@umm.edu> References: <48E20C86.90CE.001A.3@umm.edu> Message-ID: Kim, We need to know what the concentration of the antibody is before you make it 10ug/ml Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle Sent: Tuesday, September 30, 2008 9:25 AM To: histonet@pathology.swmed.edu Subject: [Histonet] PLS Help with IHC protocol Step 10: Add antibody at 10ug/ml in PBS + 0.1%BSA + 1:10 volume CAS block Can someone break this down for me in simple instructions? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Sep 30 10:53:57 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Sep 30 10:54:02 2008 Subject: [Histonet] Lot Logs In-Reply-To: <265230.1764.qm@web65714.mail.ac4.yahoo.com> References: <00f801c9230d$7b5784d0$06570181@sfmc.net> <265230.1764.qm@web65714.mail.ac4.yahoo.com> Message-ID: No, not sure my supplier even furnishes lot numbers on that. Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, September 30, 2008 9:13 AM To: histonet@lists.utsouthwestern.edu; Matt Roark Subject: Re: [Histonet] Lot Logs I never did that BUT if you have a problem during any step of your process it is a good practice to consult the supplier with the lot number of their products to find out if the problem you experienced has been reported by others also. Ren? J. --- On Tue, 9/30/08, Matt Roark wrote: From: Matt Roark Subject: [Histonet] Lot Logs To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 30, 2008, 11:01 AM Does everyone keep logs of lot numbers for their xylene and alcohol bottles? I can see keeping track of lot numbers for stains and antibodies but I can not find in the CAP checklist where they would want us to keep track of the lots of xylene and or alcohol. What does everyone else do? Thanks!! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Sep 30 10:59:22 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Sep 30 11:00:08 2008 Subject: [Histonet] Lot Logs In-Reply-To: References: <00f801c9230d$7b5784d0$06570181@sfmc.net><265230.1764.qm@web65714.mail.ac4.yahoo.com> Message-ID: Lot numbers and expiration dates should be on all supplies and most supplies will have them. We do not keep track of all lot numbers of alcohol and xylene but we do when we are running a GLP study. Each reagent and supply (manufacturer, lot number and expiration date) that is used during a GLP study is recorded and kept with the study documents for that particular study. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, September 30, 2008 9:54 AM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Matt Roark' Subject: RE: [Histonet] Lot Logs No, not sure my supplier even furnishes lot numbers on that. Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, September 30, 2008 9:13 AM To: histonet@lists.utsouthwestern.edu; Matt Roark Subject: Re: [Histonet] Lot Logs I never did that BUT if you have a problem during any step of your process it is a good practice to consult the supplier with the lot number of their products to find out if the problem you experienced has been reported by others also. Ren? J. --- On Tue, 9/30/08, Matt Roark wrote: From: Matt Roark Subject: [Histonet] Lot Logs To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 30, 2008, 11:01 AM Does everyone keep logs of lot numbers for their xylene and alcohol bottles? I can see keeping track of lot numbers for stains and antibodies but I can not find in the CAP checklist where they would want us to keep track of the lots of xylene and or alcohol. What does everyone else do? Thanks!! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paintedsplashes <@t> yahoo.com Tue Sep 30 11:00:22 2008 From: paintedsplashes <@t> yahoo.com (Jeanne Clark) Date: Tue Sep 30 11:00:28 2008 Subject: [Histonet] Control blocks for Special Stains Message-ID: <218118.41917.qm@web30706.mail.mud.yahoo.com> We are looking at making our own SS blocks from fresh tissue injected with organisms we can get from our Micro dept.? Any advice on this......what type of fresh tissue?, etc. ? Jeanne Jeanne Clark HT/MLT (ASCP) Pathology Manager Mission Hospitals 428 Biltmore Ave. Asheville, NC. 28801 828-213-1213 ? ? From victor <@t> pathology.washington.edu Tue Sep 30 11:36:02 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Sep 30 11:36:06 2008 Subject: [Histonet] Control blocks for Special Stains In-Reply-To: <218118.41917.qm@web30706.mail.mud.yahoo.com> References: <218118.41917.qm@web30706.mail.mud.yahoo.com> Message-ID: <48E25572.7040001@pathology.washington.edu> For gram stains I always used muscle since it stains yellow and the organisms stand out well. We did a spirochete once and used muscle as well. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Jeanne Clark wrote: > We are looking at making our own SS blocks from fresh tissue injected with organisms we can get from our Micro dept. > Any advice on this......what type of fresh tissue?, etc. > > Jeanne > > > Jeanne Clark HT/MLT (ASCP) > Pathology Manager Mission Hospitals > 428 Biltmore Ave. > Asheville, NC. 28801 > 828-213-1213 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ktuttle <@t> umm.edu Tue Sep 30 12:06:28 2008 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Tue Sep 30 12:06:50 2008 Subject: [Histonet] PLS Help with IHC protocol In-Reply-To: References: <48E20C86.90CE.001A.3@umm.edu> Message-ID: <48E22453.90CE.001A.3@umm.edu> Its 1.1mg/ml Another question: they are asking that I add glycerol to the antibody to prevent freezing. Wont that change my concentration? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax >>> "Patsy Ruegg" 9/30/2008 11:52 am >>> Kim, We need to know what the concentration of the antibody is before you make it 10ug/ml Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle Sent: Tuesday, September 30, 2008 9:25 AM To: histonet@pathology.swmed.edu Subject: [Histonet] PLS Help with IHC protocol Step 10: Add antibody at 10ug/ml in PBS + 0.1%BSA + 1:10 volume CAS block Can someone break this down for me in simple instructions? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From kjs <@t> Stowers-Institute.org Tue Sep 30 13:02:41 2008 From: kjs <@t> Stowers-Institute.org (Smith, Karen J) Date: Tue Sep 30 13:03:03 2008 Subject: [Histonet] Lot Logs In-Reply-To: References: <00f801c9230d$7b5784d0$06570181@sfmc.net><265230.1764.qm@web65714.mail.ac4.yahoo.com> Message-ID: Brings back memories. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, September 30, 2008 10:59 AM To: Patsy Ruegg; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; Matt Roark Subject: RE: [Histonet] Lot Logs Lot numbers and expiration dates should be on all supplies and most supplies will have them. We do not keep track of all lot numbers of alcohol and xylene but we do when we are running a GLP study. Each reagent and supply (manufacturer, lot number and expiration date) that is used during a GLP study is recorded and kept with the study documents for that particular study. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, September 30, 2008 9:54 AM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Matt Roark' Subject: RE: [Histonet] Lot Logs No, not sure my supplier even furnishes lot numbers on that. Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, September 30, 2008 9:13 AM To: histonet@lists.utsouthwestern.edu; Matt Roark Subject: Re: [Histonet] Lot Logs I never did that BUT if you have a problem during any step of your process it is a good practice to consult the supplier with the lot number of their products to find out if the problem you experienced has been reported by others also. Ren? J. --- On Tue, 9/30/08, Matt Roark wrote: From: Matt Roark Subject: [Histonet] Lot Logs To: histonet@lists.utsouthwestern.edu Date: Tuesday, September 30, 2008, 11:01 AM Does everyone keep logs of lot numbers for their xylene and alcohol bottles? I can see keeping track of lot numbers for stains and antibodies but I can not find in the CAP checklist where they would want us to keep track of the lots of xylene and or alcohol. What does everyone else do? Thanks!! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.l.hofecker <@t> Vanderbilt.Edu Tue Sep 30 13:25:43 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Tue Sep 30 13:25:46 2008 Subject: [Histonet] Vanadium Acid Fuchsin Message-ID: <898D946569A27444B65667A49C07405201DFE48F@mailbe06.mc.vanderbilt.edu> Hi everyone! I need your help. I have searched the archives and received the dreaded "0 results" answer! Does anybody have experience with Vanadium Acid Fuchsin for acidophilic neurons? I do have one reference paper but would love some first hand experience to draw from. Thanks in advance Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 From rjbuesa <@t> yahoo.com Tue Sep 30 14:06:51 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Sep 30 14:06:57 2008 Subject: [Histonet] PLS Help with IHC protocol In-Reply-To: <48E22453.90CE.001A.3@umm.edu> Message-ID: <398388.77717.qm@web65703.mail.ac4.yahoo.com> Yes, it will. Ren? J. --- On Tue, 9/30/08, Kimberly Tuttle wrote: From: Kimberly Tuttle Subject: RE: [Histonet] PLS Help with IHC protocol To: "Patsy Ruegg" , histonet@pathology.swmed.edu Date: Tuesday, September 30, 2008, 1:06 PM Its 1.1mg/ml Another question: they are asking that I add glycerol to the antibody to prevent freezing. Wont that change my concentration? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax >>> "Patsy Ruegg" 9/30/2008 11:52 am >>> Kim, We need to know what the concentration of the antibody is before you make it 10ug/ml Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle Sent: Tuesday, September 30, 2008 9:25 AM To: histonet@pathology.swmed.edu Subject: [Histonet] PLS Help with IHC protocol Step 10: Add antibody at 10ug/ml in PBS + 0.1%BSA + 1:10 volume CAS block Can someone break this down for me in simple instructions? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bdelescavage <@t> cellnetix.com Tue Sep 30 14:25:08 2008 From: bdelescavage <@t> cellnetix.com (Beth Delescavage) Date: Tue Sep 30 14:27:27 2008 Subject: [Histonet] Amyloid Controls In-Reply-To: <714726.14054.qm@web45908.mail.sp1.yahoo.com> References: <714726.14054.qm@web45908.mail.sp1.yahoo.com> Message-ID: Hi Everyone~ We are having a hard time finding Amyloid controls. Can anyone recommend a company that has good controls? Thanks~ Beth Beth Delescavage, BS, HTL (ASCP) QIHC DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From tkngflght <@t> yahoo.com Tue Sep 30 15:58:48 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Sep 30 15:58:55 2008 Subject: [Histonet] Seeking a Pathology Assistant temp tech as well as a local histologist Message-ID: <2E33A6F1E97F459989BAB186C9CEACF7@FULLSTAFF.ORG> Hi everyone-a couple of temp openings for your viewing enjoyment! Seeking a fully qualified Pathology Assistant (not a histotech who can gross but a PA) for a travel position. Please call for specifics. Also seeking a local temp tech for Western Ohio. We're trying to help a facility that really needs the support but can't quite stretch for a full travel situation. If you live in Western Ohio and have been curious about temping, this might be a simple way to get dip your toes in the pool. Confidential conversations welcome-- Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org From ccrowder <@t> vetmed.lsu.edu Tue Sep 30 19:09:00 2008 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Tue Sep 30 19:11:19 2008 Subject: [Histonet] Colored paraffin Message-ID: Several years ago we purchased colored paraffin from Polysciences. We got both pink and amethyst (purple). They had other colors. The colors were great for small biopsies. However, if we used colored cassettes (green, yellow, lavender, blue) the paraffin darkened and we could hardly see the tissue. So it's a double edge sword. If we used white cassettes all the time, the colored paraffin would be great. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From aevans <@t> wellspan.org Tue Sep 30 19:41:49 2008 From: aevans <@t> wellspan.org (Evans, Andria B.) Date: Tue Sep 30 19:59:47 2008 Subject: [Histonet] Career Opening! Message-ID: We have an opening for a full time Histotechnican/Histotechnologist M-F 8:30am - 5:00pm or 9am-5:30pm, with occasional Saturdays. We have excellent pay, benefits, retirement plan, PTO, and many other perks. We are a non-profit hospital located in York, Pennsylvania. We do about 40,000 cases a year. We are 30 minutes from Harrisburg, one hour from Baltimore, about 2 hours from Philadelphia. Go to our website at www.wellspan.org and search our careers page to submit a application. We look forward to hearing from you! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From igor.deyneko <@t> gmail.com Tue Sep 30 22:30:06 2008 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Tue Sep 30 22:30:10 2008 Subject: [Histonet] Metanil Yellow and Methyl Green Message-ID: <35e16a770809302030j1c7ada8t92e23e5deaba0d63@mail.gmail.com> Dear Histonetters! I have a question regarding a dye called Metanil Yellow, can anyone suggest a good vendor for this dye (I know Sigma and Polyscientific sell it) and what are your impressions? Second question, I have asked long time regarding Methyl Green. I narrowed it down to DAKO ready to use, Polyscientific and also Vector. I did the usual stain for 5 mins and then quick rinse with 95% ethanol, and then 10 dips in series of 2 95%ethanols, 2X 100% and then 2 mins in 2 changes of Xylene and still have inconsistencies. Can someone suggest a good protocol? Thank you very much. Igor Deyneko Infinity Pharmaceuticals In-Vivo Pharmacology Cambridge, MA 02139 From igor.deyneko <@t> gmail.com Tue Sep 30 22:36:01 2008 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Tue Sep 30 22:36:05 2008 Subject: [Histonet] Histology or IHC conferences/workshops Message-ID: <35e16a770809302036g510058e3v9afeb5ee3680012d@mail.gmail.com> Dear Histonetters! I was wondering where someone knows of any useful conferences or workshops , which deal with histological techniques, or Immunohistochemical techniques or even microscopy techniques( I heard about a place in Cape Cod), coming up in 2009??? Any information would be appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals In-Vivo Pharmacology Cambridge, MA 02139