[Histonet] Microtome calibration

[Rene J Buesa]

Dr. Girish:
 
BOTH "requirements" are rubbish invented by bureaucrats with lots of time in their hands and trying to appear concerned and knowledgeable.
 
Thickness is not necessary as long as the section is diagnostically useful or if some quantitative method as to the intensity is done in which case thickness = amount of matter, and would influence the outcome of the quantitative process.
 
Timing the tissue processor is also totally irrelevant because it does not really matter a few minutes each way during a processing protocol. More important would be to keep a record of the fixation time that is the only step really critical in tissue processing.
 
René J.

--- On Fri, 10/31/08, anhtx <@t> drreddys.com <anhtx <@t> drreddys.com> wrote:

From: anhtx <@t> drreddys.com <anhtx <@t> drreddys.com>
Subject: [Histonet] Microtome calibration
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, October 31, 2008, 2:49 AM



Dear all

Is anyone practising microtome calibration for thickness of sections cut?
Our GLP auditors are frequently asking for it.
They also suggest that automatic tissue processor time in each reagent
should be calibrated.

any comments

Regards
Dr Girish
India