[Histonet] progressive H&E staining using Gills

[Jackie M O'Connor]

Richard Allen 7211 Hematoxylin doesn't stain mucin blue, and is a 
beautiful all around nuclear stain.    I'm sure they'll send you a sample.





"Mickie Johnson" <mickie25 <@t> netzero.net> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
10/30/2008 02:16 PM
Please respond to
mickie25 <@t> netzero.net


To
"'Angela Bitting'" <akbitting <@t> geisinger.edu>, 
<histonet <@t> lists.utsouthwestern.edu>
cc

Subject
RE: [Histonet] progressive H&E staining using Gills






Angela,

Were you using Harris before? If so, the difference is that Gill
formulations stain the mucin of goblet cells quite a dark blue and many GI
docs don't like that look. They can get used to it though as ours did at
Sacred Heart Medical Center. Hope this helps.

Best Regards,
 
Mickie
 
Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
FAX   509-624-3926
Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com 
Email: mickie25 <@t> netzero.net
 
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Thursday, October 30, 2008 11:30 AM
To: histonet
Subject: [Histonet] progressive H&E staining using Gills

We just switched our H&E stain to a progressive stain using Gill II
hematoxylin. Our derm guys love it, but now our GI docs are screaming 
bloody
murder. Does anyone have suggestions for a protocol that will make both
'relatively happy'?? Thanks for letting me pick your brains again!!!



Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
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