[Histonet] Re: rat vaginal smears

[Robert Richmond]

Helen Johnson asks: >>Does anyone have a protocol for staining rat
vaginal smears with H&E?<<

Any H & E suitable for frozen sections should suffice. But it's the
wrong stain, assuming you're looking for cornification
(keratinization) of superficial squamous cells. I don't know what
stain was used traditionally, but if I were to suggest something, it
would be the ordinary Pap (Papanicolaou) stain, which stains nuclei
with hematoxylin, intermediate cells with light green or fast green,
and superficial cells with orange G. - If you buy a Pap stain
commercially, get one in which the EA50 and the orange G are separate
stains, and not combined.

Bob Richmond
Samurai Pathologist
Knoxville TN