[Histonet] rat vaginal smears

[Joseph Saby]

While cutting myh teeth in research many years ago, I performed rat vaginal smears and did indeed use the PAP stain.  It worked very nicely.




________________________________
From: "Della Speranza, Vinnie" <dellav <@t> musc.edu>
To: Helen E Johnson <hej01 <@t> health.state.ny.us>; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, October 29, 2008 5:07:50 PM
Subject: RE: [Histonet] rat vaginal smears

You didn't indicate what you will be evaluating but your request is reminiscent of the work George Papanicolau did with guinea pigs. Have you considered the pap stain? 

Here is a link that will provide a lot of information if this stain is of interest

http://www.statlab.com/Protocol/CytologyStaining.htm

I like to think of the pap stain as a 'suped up' H&E

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Helen E Johnson
Sent: Wednesday, October 29, 2008 1:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] rat vaginal smears


Does anyone have a protocol for staining rat vaginal smears with H&E?
                              Helen Johnson  (hej01 <@t> health.state.ny.us)


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