[Histonet] Histology and immuonhistochemistry of Drosophila eyesections (OT)

[Peggy Bisher]

I have students here who have been doing cryo-sectioning of
Drosophila. No one, has yet tried it in paraffin. Since they are very tiny
flies, we have a contraption that allows us to orient the flies so that
their heads are all in a row and we just cut a whole bunch at once. It looks
like a slide with a long groove in it - just wide enough to fit their heads
above it and the bodies hang down below. It seems to work just fine.

Cheers, Peggy


Margaret E. Bisher
Electron Microscopy & Histology Core Facility Manager
Department of Molecular Biology
Princeton University
Moffett Laboratory, Room 113
Princeton, New Jersey
Office: (609) 258-7026
Fax: (609) 258-8468
mbisher <@t> princeton.edu




On 10/27/08 9:46 PM, "Ingles Claire" <CIngles <@t> uwhealth.org> wrote:

>  
> Fly's eyes? What about bee's knees?
> Sorry it's almost 9pm. I'm fizzling out.
> Claire :o
> 
> ________________________________
> 
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of pam johnson
> Sent: Mon 10/27/2008 4:39 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Histology and immuonhistochemistry of Drosophila
> eyesections
> 
> 
> 
> 
> I have been approached by an investigator?who wants us to do some work on fly
> eyes. Has anyone had any experience with fly eyes in paraffin? If so can you
> please share your protocols on processing and cutting. Aren't they very
> crunchy? Do you process the whole head?
> 
> ?
> 
> Any information would be greatly appreciated.
> 
> Thanks,
> 
> 
> Pam Johnson, BS, HT (ASCP)
> Lab Manager
> Veterinary Pathology Core Lab
> St. Jude Children's Research Hospital
> 262 Danny Thomas Place
> Memphis, TN? 38105-3678
> Office - 901-595-3355
> 
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