Fw: [Histonet] PAS with Glycogen Digestion - Solution Not Working

[Bryan Llewellyn]

----- Original Message ----- 
From: "Bryan Llewellyn" <llewllew <@t> shaw.ca>
To: "McKnight, Tanisha" <tanisha.mcknight <@t> covance.com>
Sent: Friday, October 24, 2008 9:34 AM
Subject: Re: [Histonet] PAS with Glycogen Digestion - Solution Not Working


> Try porcine pancreatic alpha amylase from Sigma (A 3176).  I used the 
> amount to cover a dime 1 mm deep in 15 mL distilled water and it always 
> worked for me in an hour at room temperature.
>
> However, in general, you should base your concentration on the amount of 
> enzyme you need rather than the powder that contains the enzyme.  That is 
> 0.5 grams of a 10 units per gram powder is not the same concentration as 
> 0.5 grams of a 100 units per gram powder.
>
> Bryan Llewellyn
>
> ----- Original Message ----- 
> From: "McKnight, Tanisha" <tanisha.mcknight <@t> covance.com>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Friday, October 24, 2008 8:26 AM
> Subject: [Histonet] PAS with Glycogen Digestion - Solution Not Working
>
>
>> Hi All:
>>
>> I am in the process of working up a PAS Staining protocol. The PAS stain
>> is working fine, but I am unable to get my tissue to digest. I am using
>> .5g of Diastase in 50ml of DI water. I tried heating the solution in a
>> water bath for 30 min, 1 hour and 1.5 hours and it still looks the same
>> as the one without digestion. Could I be using the wrong Diastase? If
>> so, does anyone have any suggestions for a new product?
>>
>> Thanks,
>>
>> Tanisha Neely, HT(ASCP)
>> AP-Histology/Specimen Management
>> <mailto:tanisha.mcknight <@t> covance.com>
>>
>>
>>
>>
>> -----------------------------------------------------
>> Confidentiality Notice: This e-mail transmission
>> may contain confidential or legally privileged
>> information that is intended only for the individual
>> or entity named in the e-mail address. If you are not
>> the intended recipient, you are hereby notified that
>> any disclosure, copying, distribution, or reliance
>> upon the contents of this e-mail is strictly prohibited.
>>
>> If you have received this e-mail transmission in error,
>> please reply to the sender, so that we can arrange
>> for proper delivery, and then please delete the message
>> from your inbox. Thank you.
>>
>>
>
>
> --------------------------------------------------------------------------------
>
>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>