[Histonet] PAS with Glycogen Digestion - Solution Not Working

[renafail <@t> bellsouth.net]

 I had far more consistent results with amylase type VI (sigma) than diastase, recommended by someone from histonet when I had problems with diastase
Ren Fail
 -------------- Original message from "McKnight, Tanisha" <tanisha.mcknight <@t> covance.com>: --------------


> Hi All:
>  
> I am in the process of working up a PAS Staining protocol. The PAS stain
> is working fine, but I am unable to get my tissue to digest. I am using
> .5g of Diastase in 50ml of DI water. I tried heating the solution in a
> water bath for 30 min, 1 hour and 1.5 hours and it still looks the same
> as the one without digestion. Could I be using the wrong Diastase? If
> so, does anyone have any suggestions for a new product?
>  
> Thanks,
>  
> Tanisha Neely, HT(ASCP)
> AP-Histology/Specimen Management
>    
> 
> 
> 
> 
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