[Histonet] MAOB IHC on either frozen or FFPE sections

[Merced Leiker]

I've never worked with MAOB, but what I do when I run into similar 
situations is to do a 1:50 and 1:200 dilution in parallel to start getting 
a feel for the antibody.  (Make sure to use antigen retrieval if you need 
it on the FFPE sections; apologies if you already knew that).

Yeah I, too, think it's weird that they illustrate the antibody at a 
particular concentration and then tell you they never determined the stock 
concentration!

Merced

--On Friday, October 24, 2008 11:41 AM -0400 "Connolly, Brett M" 
<brett_connolly <@t> merck.com> wrote:

> Has anyone done it? I have an antibody from Abnova. They illustrate
> staining of FFPE kidney at 3ug/mL, but when I asked what the stock Ab
> concentration is they say they never determined that. Go figure? I'm
> looking for a starting point dilution.
> Thanks,
> Brett
>
> Brett M. Connolly, Ph.D.
> Research Fellow, Imaging Research
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> PH 215-652-2501 fax. 215-993-6803
> e-mail. brett_connolly <@t> merck.com
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Merced M Leiker
Research Technician II
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School of Medicine and Biomedical Sciences
State University of New York at Buffalo
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