[Histonet] PAS with Glycogen Digestion - Solution Not Working

McKnight, Tanisha tanisha.mcknight <@t> covance.com
Fri Oct 24 10:26:17 CDT 2008


Hi All:
 
I am in the process of working up a PAS Staining protocol. The PAS stain
is working fine, but I am unable to get my tissue to digest. I am using
.5g of Diastase in 50ml of DI water. I tried heating the solution in a
water bath for 30 min, 1 hour and 1.5 hours and it still looks the same
as the one without digestion. Could I be using the wrong Diastase? If
so, does anyone have any suggestions for a new product?
 
Thanks,
 
Tanisha Neely, HT(ASCP)
AP-Histology/Specimen Management
 <mailto:tanisha.mcknight <@t> covance.com>  




-----------------------------------------------------
Confidentiality Notice: This e-mail transmission 
may contain confidential or legally privileged 
information that is intended only for the individual 
or entity named in the e-mail address. If you are not 
the intended recipient, you are hereby notified that 
any disclosure, copying, distribution, or reliance 
upon the contents of this e-mail is strictly prohibited. 

If you have received this e-mail transmission in error, 
please reply to the sender, so that we can arrange 
for proper delivery, and then please delete the message 
from your inbox. Thank you.



More information about the Histonet mailing list