[Histonet] Re: anti-Human CD68

[Kim Merriam]

Hi Chris-
Thanks for the insight, I never would have even thought to check that the mouse isotypes were different.  I will give it a look!
Kim

 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 




________________________________
From: C.M. van der Loos <c.m.vanderloos <@t> amc.uva.nl>
To: histonet <@t> lists.utsouthwestern.edu
Cc: kmerriam2003 <@t> yahoo.com
Sent: Tuesday, October 21, 2008 1:54:02 PM
Subject: RE: anti-Human CD68


Hi Kim,
I wondering if your question for an anti-CD68 antibody raised in rabbit or goat is because of double staining plans. If so, please realize that the Dako antibody, clone PG-M1, is a mouse IgG3. That easily allows double staining with other mouse IgG1 or IgG2a antibodies. Go to Southern Biotechnology Associates (www. southernbiotech.com) for specific second step antibodies conjugated with alk. phos., peroxidase or biotin. Just a guess!
Cheers,
Chris
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands

Date: Mon, 20 Oct 2008 11:03:44 -0700 (PDT)
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: [Histonet] anti-Human CD68
To: Histonet histonet <@t> lists.utsouthwestern.edu

Hello,

I am looking for a CD68 antibody that will work in Human FFPE tissues, but it needs to be from a species other than mouse (preferably rabbit or goat). Any recommendations?

Thanks,
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA