[Histonet] Elastin Aldehyde Fuchsin

[Adam Boanas]

Hello,

 

I have been using Gomori`s Aldehyde Fuchsin to stain human skin for
elastin. The stain works fine and is clear and specific. I am using
light green as a counterstain. My problem is that once stained, the
slides have an excess granular purple "dotted" appearance upon the
entire surface of the slide. This is not limited to the tissue position
upon the slide, but across the clear glass too. We have tried using both
superfrost plus charged slides and uncharged slides but the problem
still is present. This precipitate is spoiling an otherwise great stain.
Does anyone have any idea about what this is and how it could be
prevented? (Our next guess would be to re-filter the fuchsin solution
prior to use every time)

 

Many thanks

Adam

 

Adam Boanas 
Histology Manager 
Renovo Ltd 

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