[Histonet] More bone questions

[Liz Chlipala]

I'm working with a client who it is growing cells on a demineralized
bone matrix.  We have processed the tissue both manually and on a VIP
and we are seeing that the cells are not attached to the bone and that
they are separated away from the bone.  We are trying to figure out if
this is a processing artifact and what we can do to prevent it.  The
cells appear to follow the structure of the bone its just that they are
separated away from the bone and should be (in a perfect world) attached
to or laying on top of the edge of the bone.  If someone has worked with
these types of samples before and has advice that would be great.
 
thanks in advance
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 682-3949
fax (303) 682-9060
liz <@t> premierlab.com
www.premierlab.com <http://www.premierlab.com/> 
 
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