[Histonet] histology ink washing off

[Leslie Chen]

Thank you everyone for your help.  It seems the consensus is to use acetic acid as a mordant which worked.  I have tried just blotting the tissue, but it never seems to completely "dry".  The tissue has been fixed in gluteraldehyde and stained with the LacZ protocol and then post-fixed with PFA.  This may be why the tissue never seems to dry.  Has anyone experienced this before with LacZ staining? This has also caused some problems when frozen sectioning.  Thank you!

Leslie

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