AW: [Histonet] histology ink washing off

[Gudrun Lang]

We dry and heat the tissue surface with a hair-dryer at the grossing. It
sounds a little bit rigid, but it works and the tissue margins are easily
seen in the slide. The excess dye also will be solved in the NBF, but there
sticks enough on the tissuemargins.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
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Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Leslie
Chen
Gesendet: Freitag, 10. Oktober 2008 22:31
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] histology ink washing off

Hello,
I am having a problem with histology tissue marking ink washing off
specimens in water, PFA, or 70% alcohol.  This is before the tissue even
gets processed for paraffin embedding. Does anyone have any suggestions on
how to keep the ink from coming off?  Thank you.

Leslie

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