[Histonet] section thickness

[Rene J Buesa]

General routine = 5 µm
Brain = at least 8 µm
René J.

--- On Thu, 10/9/08, LINDA MARGRAF <LINDA.MARGRAF <@t> childrens.com> wrote:

From: LINDA MARGRAF <LINDA.MARGRAF <@t> childrens.com>
Subject: [Histonet] section thickness
To: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, October 9, 2008, 1:49 PM

Histonetters:
Here's a query from Janet that she couldn't get to post to the list.
Please respond to the list so she'll see the response. Thanks.
I would like to poll the group regarding routine practices for thickness of
paraffin sections. I am particularly interested in brain, spinal cord, lymph
node, tonsil, and renal biopsy 
Most references range from 3-6 um for routine tissues with 4-5um being most
prevalent. What do you use? Which tissues do you cut at a different thickness
routinely? 
I am not referring to special stains as the protocol would prescribe the
thickness.
Thanks for your help.
Janet Keeping A.R.T., B. Voc. Ed
Instructor
Medical Laboratory Sciences
 
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