[Histonet] section thickness

[LINDA MARGRAF]

Histonetters:
Here's a query from Janet that she couldn't get to post to the list. Please respond to the list so she'll see the response. Thanks.
I would like to poll the group regarding routine practices for thickness of paraffin sections. I am particularly interested in brain, spinal cord, lymph node, tonsil, and renal biopsy 
Most references range from 3-6 um for routine tissues with 4-5um being most prevalent. What do you use? Which tissues do you cut at a different thickness routinely? 
I am not referring to special stains as the protocol would prescribe the thickness.
Thanks for your help.
Janet Keeping A.R.T., B. Voc. Ed
Instructor
Medical Laboratory Sciences
 
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