[Histonet] Does anyone have any ideas on the use of softenerondecal blocks. Does this help 'soften' them? If so how?

[Walzer Susan]

We face the block and put it in a dish with rapid decal solution. For
tough blocks like uterus we soak them in a solution of water,
ammonia(which is great for bloody tissue) and liquid soap.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie
Reilly
Sent: Friday, October 03, 2008 7:58 PM
To: 'Piero Nelva'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Does anyone have any ideas on the use of
softenerondecal blocks. Does this help 'soften' them? If so how?

Floating the trimmed/faced block on the waterbath for 2 minutes helps.
Then put them on wet ice to cool.

Regards,   Laurie.

Mr. Laurie REILLY
Histopathology
School of  Veterinary and Biomedical Sciences
James Cook University
Townsville  Qld.  4811
Australia.

Phone 07 4781 4468

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Piero
Nelva
Sent: Friday, 3 October 2008 7:16 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Does anyone have any ideas on the use of
softener
ondecal blocks. Does this help 'soften' them? If so how?

Hi Sarah

I don't use a softener as such, but have had good results putting
trimmed 
blocks on a water soaked tissue on the bench for 30 min or so just
before 
cooling and sectioning. I find it is more effective than an ice bath at 
softening tissues.

REgards

Piero Nelva
Anatomical Pathology
Monash Medical Centre
Australia
----- Original Message ----- 
From: "SARAH REEVES" <SARAH.REEVES <@t> ekht.nhs.uk>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, October 03, 2008 12:34 AM
Subject: [Histonet] Does anyone have any ideas on the use of softener on

decal blocks. Does this help 'soften' them? If so how?


Does anyone have any ideas on the use of softener on decal blocks. Does
this

help 'soften' them? If so how?

Thanks in advance

Sarah


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