[Histonet] Isopropyl vs Ethanol

[Walzer Susan]

We don't use it for processing but we use it at the end of the stain set
up and coverslip out of it using Clearium mounting medium (it is misable
with alcohol). We did it to keep out fingers out of xylene as it is a
neurotoxin. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amos
Brooks
Sent: Friday, October 03, 2008 8:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Isopropyl vs Ethanol

Hi,
   Is there anyone who uses exclusively isopropyl alcohol to process
with
rather than ethanol? I have had great results with isopropyl alcohol for
some tissues, but I wanted to know if there are any pitfalls to using it
exclusively for processing. I've noticed that isopropyl is much cheaper
than
ethanol, so it leads me to wonder why ethanol is so prevalant for
dehydration.

Thanks,
Amos Brooks
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