[Histonet] Antigen Retrieval

[Sebree Linda A.]

Try using a laboratory pressure cooker like the Decloaking Chamber from
Biocare Medical.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patten,
Nicole (NIH/NIAAA) [F]
Sent: Friday, October 03, 2008 12:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Antigen Retrieval

Hi-

 

I am new to using IHC and I need some advice on Antigen Retrieval. I
usually do it in an autoclave for 15min at 121C for FFPE human brain
tissue (in Citrate or Tris Buffer), but by the end my tissue looks
pretty bad and parts have even fallen off. The tissue is fixed on
SuperFrost Plus Slides. 

 

Any suggestions would be really helpful. Thanks!

 

N. Patten
Post-Baccalaureate Fellow/IRTA
National Institutes of Health

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