[Histonet] Winterize formalin?

[Sate Hamza]

Thank you Terry .. and thanks to everyone who replied to me about this ...

Sate

On Thu, Oct 2, 2008 at 10:37 AM, Marshall Terry Dr, Consultant
Histopathologist <Terry.Marshall <@t> rothgen.nhs.uk> wrote:

> 1.5% sounds homeopathic to me.
>
> There will be more depression of freezing point due to the salts in
> buffered formalin than anything.
>
> In the excerpt you gave Sate, antifreeze worked best (surprise
> surprise).
>
> It's hard to imagine with modern transport that things are going to hang
> around for long enough and at such a low temperature that crystals are
> going to form.
>
> Terry
>
> PS I had a picture of the freezing artefact once but have lost it. If
> someone has one I sure would appreciate it.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sate
> Hamza
> Sent: 02 October 2008 15:58
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Winterize formalin?
>
> Dear Histonetters,
>
> A colleague from Boston posted this inquiry to our dermatopathology
> mailing
> list: "Does anyone [have] the formula to winterize formalin? We were
> adding
> 15 ml of alcohol to 1000 ml of 10% buffered formalin. It used to work
> but is not working now at -20" ..
>
> Interestingly, I found out that we do not "winterize" formalin up here
> in Winnipeg, Canada (also jokingly referred to as "Winterpeg"). I am
> wondering if anyone on the list "winterizes" formalin .. I found the
> following abstract of a Japanese paper by searching in Pubmed
>
> http://www.ncbi.nlm.nih.gov/pubmed/3692431
>
> Thank you ..
>
>


-- 
Sate Hamza, MD, FRCPC
Dermatopathologist
Winnipeg, Canada