From pieronelva01 <@t> bigpond.com Wed Oct 1 03:45:49 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Wed Oct 1 03:45:27 2008 Subject: [Histonet] Histology or IHC conferences/workshops References: <35e16a770809302036g510058e3v9afeb5ee3680012d@mail.gmail.com> Message-ID: Hi Igor There is a Frozen section workshop running in Melbourne, Australia as part of the Australian Instituse of Medical Science National meeting (aims.org.au and follow the links). It is on on mid October. The Histology Group of Victoria (hgv.org.au) also runs regular workshops and conferences for histology scientists. REgards Piero Nelva Anatomical Pathology Monash Medical Centre Melbourne Australia ----- Original Message ----- From: "Igor Deyneko" To: Sent: Wednesday, October 01, 2008 1:36 PM Subject: [Histonet] Histology or IHC conferences/workshops > Dear Histonetters! > I was wondering where someone knows of any useful conferences or workshops > , > which deal with histological techniques, or Immunohistochemical techniques > or even microscopy techniques( I heard about a place in Cape Cod), coming > up > in 2009??? Any information would be appreciated. > Thank you. > Igor Deyneko > Infinity Pharmaceuticals > In-Vivo Pharmacology > Cambridge, MA 02139 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.7.5/1698 - Release Date: 9/29/2008 7:25 PM From ploykasek <@t> phenopath.com Wed Oct 1 09:15:07 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Oct 1 09:15:19 2008 Subject: [Histonet] Histology or IHC conferences/workshops In-Reply-To: <35e16a770809302036g510058e3v9afeb5ee3680012d@mail.gmail.com> Message-ID: Hi Igor. I am not sure of specific topics for 2009 but you should look at the websites of ASCP, NSH, and TNT (teleconference network of TX). All of these entities sponsor teleconferences. NSH will be in Birmingham, AL 2009 & there are always great seminars there. Patti Loykasek > Dear Histonetters! > I was wondering where someone knows of any useful conferences or workshops , > which deal with histological techniques, or Immunohistochemical techniques > or even microscopy techniques( I heard about a place in Cape Cod), coming up > in 2009??? Any information would be appreciated. > Thank you. > Igor Deyneko > Infinity Pharmaceuticals > In-Vivo Pharmacology > Cambridge, MA 02139 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Sarah.Hale <@t> uvm.edu Wed Oct 1 09:16:31 2008 From: Sarah.Hale <@t> uvm.edu (Hale, Sarah) Date: Wed Oct 1 09:16:39 2008 Subject: [Histonet] Histoclear vs. Xylene Message-ID: <36830148D2AC3E48A318C1436C89462B088BEDE9@MED05.med.uvm> Are there any opinions as to using histoclear vs. xylene? Thanks! Sarah From rjbuesa <@t> yahoo.com Wed Oct 1 09:39:17 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 1 09:39:24 2008 Subject: [Histonet] Histoclear vs. Xylene In-Reply-To: <36830148D2AC3E48A318C1436C89462B088BEDE9@MED05.med.uvm> Message-ID: <625763.32143.qm@web65705.mail.ac4.yahoo.com> Sarah: Histoclear, also known as Histolene is a d-Limonene based xylene substitute and there are 13 opinions about it in HistoNet that I will try to summarize for you: 1-not as good as Xylene to dewax before IHC 2-has a strong odor 3-hardens brain, liver and spleen 4-not good for cover slipping 5-incompatible with xylene or toluene based cover medium 6- has produced skin rashes 7- has produced headache problems 8- fades hematoxylin 9-avoid Strong oxidizer when using it 10- has to be used with Histomount or Omnimount medium 11- generally speaking is a poor dewaxing agent 12- one posting concluded as "avoid it" On the other hand Wynnchuk (1994) says is as good as xylene, but? Faolain et al (2005) wrote that it does not dewax well. I am sure that there will be some that "love it", but I personally would not use it based on the above. Besides that it costs $59.29/gal or 1.47 times as much as xylene. Ren? J. --- On Wed, 10/1/08, Hale, Sarah wrote: From: Hale, Sarah Subject: [Histonet] Histoclear vs. Xylene To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 1, 2008, 10:16 AM Are there any opinions as to using histoclear vs. xylene? Thanks! Sarah _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Oct 1 12:16:28 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Oct 1 12:16:55 2008 Subject: [Histonet] tissue swelling out of block Message-ID: Hi all, a friend recently got a job in a veterinary histo and has some troubles in cutting. He tells about paraffinblocks, where the tissue is swelling out while warming like a small hill. I referred to the possibilty of taking up humidity that causes swelling. Do you know other causes for this problem? Thanks in advance Gudrun Lang From jqb7 <@t> cdc.gov Wed Oct 1 12:21:50 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Oct 1 12:22:06 2008 Subject: [Histonet] tissue swelling out of block In-Reply-To: References: Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C2E7@LTA3VS011.ees.hhs.gov> Is he saying that he embeds processed tissue and removes the chilled paraffin block from the base mold that it then swells up in the paraffin? Before it is trimmed and placed on ice? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Wednesday, October 01, 2008 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue swelling out of block Hi all, a friend recently got a job in a veterinary histo and has some troubles in cutting. He tells about paraffinblocks, where the tissue is swelling out while warming like a small hill. I referred to the possibilty of taking up humidity that causes swelling. Do you know other causes for this problem? Thanks in advance Gudrun Lang From rfields <@t> gidocs.net Wed Oct 1 12:29:44 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Wed Oct 1 12:30:03 2008 Subject: [Histonet] tissue swelling out of block Message-ID: <07732CE52EC3174AB891DE1C62DB4D8F43EC6F@GIEXCHANGE.gidocs.net> The major problem in a veterinary histo lab is generally 1 processor to process all types of tissues and species. The lab I can from had this problem; you can only optimize the schedule if you limit your tissues. Some are under, some are over; and a small percentage are just right. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Wednesday, October 01, 2008 12:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue swelling out of block Hi all, a friend recently got a job in a veterinary histo and has some troubles in cutting. He tells about paraffinblocks, where the tissue is swelling out while warming like a small hill. I referred to the possibilty of taking up humidity that causes swelling. Do you know other causes for this problem? Thanks in advance Gudrun Lang From tjasper <@t> copc.net Wed Oct 1 12:32:11 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Wed Oct 1 12:32:18 2008 Subject: [Histonet] tissue swelling out of block References: Message-ID: <90354A475B420441B2A0396E5008D496731785@copc-sbs.COPC.local> Hi Gudrun, Is your friend soaking blocks in ice water before cutting? This is common in animal histology and over soaking before cutting will cause swelling. Different tissue types will swell more quickly than others, but over soaking can eventually affect all tissue. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Wednesday, October 01, 2008 10:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue swelling out of block Hi all, a friend recently got a job in a veterinary histo and has some troubles in cutting. He tells about paraffinblocks, where the tissue is swelling out while warming like a small hill. I referred to the possibilty of taking up humidity that causes swelling. Do you know other causes for this problem? Thanks in advance Gudrun Lang From TJJ <@t> Stowers-Institute.org Wed Oct 1 12:35:07 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Oct 1 12:35:44 2008 Subject: [Histonet] Re: PLS Help with IHC protocol Message-ID: Kimberly, you asked: >>Step 10: Add antibody at 10ug/ml in PBS + 0.1%BSA + 1:10 volume CAS block Can someone break this down for me in simple instructions?<< And >>Its 1.1mg/ml Another question: they are asking that I add glycerol to the antibody to prevent freezing. Wont that change my concentration?<< Some antibody companies request you add equal parts of glycerol to the antibody stock to prevent freeze/thaw cycles which is not good for antibody integrity. If you add equal parts of glycerol, your concentration would now be .55 mg/ml. In order to get ug/ml, you will need to convert .55 mg/ml to ug/ml. You do that by multiplying by 1000, therefore .55 mg/ml = 550 ug/ml. In order to get 10 ug/ml concentration, you would divide 550 by 10, and you get 55. So you would use a 1:55 dilution of antibody/glycerol stock. The PBS/BSA/CAS block is a stock solution which you will then use as the diluent for the antibody. Some folks make it up using commercial antibody diluent. Some folks use whatever they use for the normal serum block (say 5-10% serum from the species source the secondary antibody is made in, i.e. normal goat serum if your secondary antibody is goat anti-rabbit). There is no one best diluent for antibodies. If you want to use the PBS/BSA/CAS block, go ahead. But you don't have to if you want to simplify things. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From mwich <@t> 7thwavelabs.com Wed Oct 1 14:14:13 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Wed Oct 1 14:14:18 2008 Subject: [Histonet] Okajima's stain Message-ID: <62A8156F8071C8439080D626DF8C33A602E4EC@wave-mail.7thwave.local> This is probably a really obvious question, but when doing Okajima's stain for hemoglobin, is a blood smear an adequate control? Thanks for any advice. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From rjbuesa <@t> yahoo.com Wed Oct 1 14:14:37 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 1 14:14:40 2008 Subject: [Histonet] tissue swelling out of block In-Reply-To: Message-ID: <333937.57866.qm@web65710.mail.ac4.yahoo.com> The tissue probably is incompletely infiltrated and absorbs humidity. Ren? J. --- On Wed, 10/1/08, Gudrun Lang wrote: From: Gudrun Lang Subject: [Histonet] tissue swelling out of block To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 1, 2008, 1:16 PM Hi all, a friend recently got a job in a veterinary histo and has some troubles in cutting. He tells about paraffinblocks, where the tissue is swelling out while warming like a small hill. I referred to the possibilty of taking up humidity that causes swelling. Do you know other causes for this problem? Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfranci <@t> rigel.com Wed Oct 1 16:48:45 2008 From: cfranci <@t> rigel.com (Christian Franci) Date: Wed Oct 1 16:48:49 2008 Subject: [Histonet] myeloperoxidase Message-ID: <1895776bf35101f0c9846272f66006c1@rigel.com> Hello histoneters! I've been asked to stain some mouse liver sections with this myeloperoxidase stain. I've never done it so, i was wondering if any of you out there in Histoland had a good protocol for it? Thanks in advance! cheers, Chris From marktarango <@t> gmail.com Wed Oct 1 17:14:41 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Oct 1 17:14:46 2008 Subject: [Histonet] Tissue Processor Message-ID: <5b6eb13e0810011514n7e2e96d6q30b4b63a34929fbc@mail.gmail.com> Does anyone happen to have a good used tissue processor for sale? I'm also interested in an embedding center and microtome. Thanks Mark From cmmathis1 <@t> bellsouth.net Wed Oct 1 17:50:54 2008 From: cmmathis1 <@t> bellsouth.net (cmmathis1@bellsouth.net) Date: Wed Oct 1 17:51:02 2008 Subject: [Histonet] Sca-1 against rat In-Reply-To: Message-ID: <100120082250.2774.48E3FECD000D370000000AD622230680329B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> Hello all, I am looking for an anti-rat Sca-1 (Ly6A/E) antibody. If you know of one, I would be so greatful for the information. So far I have found lots of rat anti-mouse Sca-1. I have done mouse on mouse staining, but how do you do rat on rat staining? Any information would be helpful. Cathy From cmmathis1 <@t> bellsouth.net Wed Oct 1 17:54:11 2008 From: cmmathis1 <@t> bellsouth.net (cmmathis1@bellsouth.net) Date: Wed Oct 1 17:54:16 2008 Subject: [Histonet] cutting cotton fibers Message-ID: <100120082254.12069.48E3FF9300098CE200002F2522230680329B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> I have a question for maybe some of the plant histotechs out there. How can you best section cotton fibers? And yes I may be doing this soon. Thanks so very much, Cathy From marktarango <@t> gmail.com Wed Oct 1 20:05:24 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Oct 1 20:05:28 2008 Subject: [Histonet] cutting cotton fibers In-Reply-To: <100120082254.12069.48E3FF9300098CE200002F2522230680329B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> References: <100120082254.12069.48E3FF9300098CE200002F2522230680329B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> Message-ID: <5b6eb13e0810011805q276dc155ld843c0e4a0f9134e@mail.gmail.com> I'll be following this thread very closely haha On 10/1/08, cmmathis1@bellsouth.net wrote: > > I have a question for maybe some of the plant histotechs out there. How > can you best section cotton fibers? And yes I may be doing this soon. > Thanks so very much, > Cathy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From RSRICHMOND <@t> aol.com Wed Oct 1 22:19:44 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Oct 1 22:19:48 2008 Subject: [Histonet] brachytherapy seeds in prostates Message-ID: Peggy Wenk asked three days ago for information about radioactive brachytherapy "seeds" in prostate tissue received in surgical pathology. I have seen one such case, about four years ago. I put the "seeds" in a flatbed scanner and got a good clear picture of them, if you want it. Three radioisotopes are used for this purpose. Iodine 125 has a half life of 60 days. Iridium 192 has a half live of about 74 days. Palladium 103 has a half life of about 17 days. I was eventually able to find out which of the three isotopes my specimen contained, and when the "seeds" were inserted, much too long ago to be of any concern. In my experience, hospital radiation safety officers are not much concerned about possible hazards to pathologists and histotechnologists from this material. As with so many other safety issues, Good Management considers us beneath consideration. Bob Richmond Samurai Pathologist Knoxville TN ******************* Subject: [Histonet] Need volunteers for lab study Peggy Wenk I'm working on a research project for my master's class on safety management. It's involving brachytherapy seeds that are sometimes found in TURPS and whole prostates received in histology (grossing and sectioning). I'm gathering data as to how radioactive these seeds are X number of months after implantation. I'm finding out that most Radiation Safety Officers in most hospitals don't know that histology receives prostates with these seeds in them. Nor does Brachytherapy - they're assuming that once the seeds are implanted, they are in the patient for life. And no one knows if the seeds are still radioactive when we receive them in grossing and during sectioning. And I can't find any articles on the topics, expect that the NRC (National Regulatory Commission) says if the lab receives radioactive specimens, then we are under the same guidelines for radiation as, say, brachytherapy, nuclear medicine, etc. What I need are some other hospitals in the US to help me gather information as to number of times these seeds are received. For this class, I just need help gathering information in October and November. Please email me directly - If you are willing to: - let me know when you receive one/some of these seeds in a whole prostate or TURP - how many total number of whole prostates and TURPS you received in Oct and Nov (I'm hoping a quick computer search would work) - and if you are willing to do a little more checking when you receive any seeds, such as - patient history as to when the seeds were implanted (pathologist hopefully will get this history), and - if possible, if they are iodine (I-125) or palladium (Pd-103) (again, maybe the pathologist will find this on the patient's history). I do NOT need any information about the patient, so don't worry about HIPPA. I know I won't get that many cases at our hospital over the next two months (research paper due in December), so I'm hoping for more hospitals will participate, so I get better data. I'm particularly interested in hospitals that HAVE received seeds in the past, as I'm guessing these labs are more likely to receive the seeds in the future. Thanks in advance. Peggy Wenk Lpwenk@sbcglobal.net From abright <@t> brightinstruments.com Thu Oct 2 06:55:11 2008 From: abright <@t> brightinstruments.com (Alan Bright) Date: Thu Oct 2 07:27:59 2008 Subject: [Histonet] cutting cotton fibers In-Reply-To: <100120082254.12069.48E3FF9300098CE200002F2522230680329B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> References: <100120082254.12069.48E3FF9300098CE200002F2522230680329B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> Message-ID: <3EFBB875DEE1994FB040A0B099F3AC8A0ACCBC@BRIGHT-SBS.Bright.local> Dear Cathy, I have sectioned many types of fibers in a cryostat, would that be acceptable for what you require? If so I can draft up some instructions for you and any others interested. Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cmmathis1@bellsouth.net Sent: 01 October 2008 23:54 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting cotton fibers I have a question for maybe some of the plant histotechs out there. How can you best section cotton fibers? And yes I may be doing this soon. Thanks so very much, Cathy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Oct 2 08:06:27 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Oct 2 08:06:31 2008 Subject: [Histonet] myeloperoxidase In-Reply-To: <1895776bf35101f0c9846272f66006c1@rigel.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A391A@IS-E2K3.grhs.net> I used a Sigma kit to do this stain. The procedure was in the kit and the stain was easy to do. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christian Franci Sent: Wednesday, October 01, 2008 4:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] myeloperoxidase Hello histoneters! I've been asked to stain some mouse liver sections with this myeloperoxidase stain. I've never done it so, i was wondering if any of you out there in Histoland had a good protocol for it? Thanks in advance! cheers, Chris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CHRISH <@t> HEALTHCARESCOUTS.COM Thu Oct 2 08:08:10 2008 From: CHRISH <@t> HEALTHCARESCOUTS.COM (Chris Handrahan) Date: Thu Oct 2 08:15:11 2008 Subject: [Histonet] Bench, IHC, Supervisor and Mgmt Opportunities Nationwide Message-ID: The #1 Recruiter for Laboratory/Biotech Specialists Healthcare Scouts is a medical laboratory/biotech recruitment firm that places professionals in full-time, permanent positions throughout the United States. As the leading nationally recognized permanent placement solution for medical laboratory/biotech professionals, Healthcare Scouts places candidates in a wide range of positions including: * Clinical Laboratory Scientists * Cytogenetic Technologists * Cytotechnologists * Histology Supervisors * Histotechnicians/ Histotechnologists * Lab Supervisors * Medical Technologists * Pathologists * Pathologist Assistants Let us Work on Your Career! Chris Handrahan Managing Director of Allied Health Healthcare Scouts 800-708-0605 office 321-231-5427 cell chrish@healthcarescouts.com www.healthcarescouts.com From PMonfils <@t> Lifespan.org Thu Oct 2 09:12:40 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Oct 2 09:12:50 2008 Subject: [Histonet] Control blocks for Special Stains In-Reply-To: <218118.41917.qm@web30706.mail.mud.yahoo.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C63@LSRIEXCH1.lsmaster.lifespan.org> I have made gram controls by injecting tissue a number of times. I like to use lung because it is full of small voids where the injected material can collect without damaging the tissue. If you inject into a more solid tissue like liver or kidney or muscle, the injection necessarily forces the tissue apart and creates one large void. I purchased my cell cultures from a biological supply company like www.ctvalleybio.com . They offer a good variety of forms (cocci, bacilli) in both gram+ and gram- types, so you can pretty much custom design your control. Make sure you get cultures grown on broth, not plates. Spin the culture down to concentrate the organisms for injection. Inject the tissue fresh (unfixed). Push the needle almost all the way through, then slowly inject as you withdraw the needle. Then just drop the tissue into formalin. Works very well. From froyer <@t> bitstream.net Thu Oct 2 09:19:32 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Oct 2 09:19:44 2008 Subject: [Histonet] Tissue Processor In-Reply-To: <5b6eb13e0810011514n7e2e96d6q30b4b63a34929fbc@mail.gmail.com> References: <5b6eb13e0810011514n7e2e96d6q30b4b63a34929fbc@mail.gmail.com> Message-ID: <235D13F6BFD248C899F1C94F2BF41B06@Ford> You did not identify yourself or your location. Are you a Dealer/Broker or an end user located in a laboratory setting? Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Wednesday, October 01, 2008 5:15 PM To: histonet Subject: [Histonet] Tissue Processor Does anyone happen to have a good used tissue processor for sale? I'm also interested in an embedding center and microtome. Thanks Mark _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SARAH.REEVES <@t> ekht.nhs.uk Thu Oct 2 09:34:38 2008 From: SARAH.REEVES <@t> ekht.nhs.uk (SARAH REEVES) Date: Thu Oct 2 09:38:23 2008 Subject: [Histonet] Does anyone have any ideas on the use of softener on decal blocks. Does this help 'soften' them? If so how? Message-ID: <48E4EA0E020000B500002A23@ekhgwia.ekht.nhs.uk> Does anyone have any ideas on the use of softener on decal blocks. Does this help 'soften' them? If so how? Thanks in advance Sarah ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals University NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** From ratliffjack <@t> hotmail.com Thu Oct 2 09:47:56 2008 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Oct 2 09:48:05 2008 Subject: [Histonet] Fluorescent Bone Labeling Protocols for Goat Message-ID: Does anyone have a protocol they would be willing to share for the fluorescent labeling of bone in goat? I am specifically interested in dosing amounts (mg/kg) and dosing schedule. Jack _________________________________________________________________ Stay up to date on your PC, the Web, and your mobile phone with Windows Live. http://clk.atdmt.com/MRT/go/msnnkwxp1020093185mrt/direct/01/ From dermpathsy <@t> gmail.com Thu Oct 2 09:58:06 2008 From: dermpathsy <@t> gmail.com (Sate Hamza) Date: Thu Oct 2 09:58:10 2008 Subject: [Histonet] Winterize formalin? Message-ID: <8854ff80810020758p488d4c1bpa88cb7edae120968@mail.gmail.com> Dear Histonetters, A colleague from Boston posted this inquiry to our dermatopathology mailing list: "Does anyone [have] the formula to winterize formalin? We were adding 15 ml of alcohol to 1000 ml of 10% buffered formalin. It used to work but is not working now at -20" .. Interestingly, I found out that we do not "winterize" formalin up here in Winnipeg, Canada (also jokingly referred to as "Winterpeg"). I am wondering if anyone on the list "winterizes" formalin .. I found the following abstract of a Japanese paper by searching in Pubmed http://www.ncbi.nlm.nih.gov/pubmed/3692431 Thank you .. Sate -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada From marktarango <@t> gmail.com Thu Oct 2 10:29:18 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Oct 2 10:29:23 2008 Subject: [Histonet] Tissue Processor In-Reply-To: <235D13F6BFD248C899F1C94F2BF41B06@Ford> References: <5b6eb13e0810011514n7e2e96d6q30b4b63a34929fbc@mail.gmail.com> <235D13F6BFD248C899F1C94F2BF41B06@Ford> Message-ID: <5b6eb13e0810020829k147347e8n2a7572ceddd67537@mail.gmail.com> My name is Mark Tarango and I'm the end user. The lab will be in Beverly Hills, Ca. Thanks On Thu, Oct 2, 2008 at 7:19 AM, Ford Royer wrote: > You did not identify yourself or your location. Are you a Dealer/Broker or > an end user located in a laboratory setting? > > Ford M. Royer, MT(ASCP) > Minnesota Medical, Inc. > 7177 Madison Ave. W. > Golden Valley, MN 55427-3601 > CELL: 612-839-1046 > Phone: 763-542-8725 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark > Tarango > Sent: Wednesday, October 01, 2008 5:15 PM > To: histonet > Subject: [Histonet] Tissue Processor > > Does anyone happen to have a good used tissue processor for sale? I'm > also > interested in an embedding center and microtome. > > Thanks > > Mark > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jfrancis <@t> platinumselect.org Thu Oct 2 10:53:30 2008 From: jfrancis <@t> platinumselect.org (John Francis) Date: Thu Oct 2 10:51:02 2008 Subject: [Histonet] jim Call me the NM client would like to interview today !!!!! Message-ID: <20081002155050.AD88D2E381E9@server1.platinumselect.org> John Francis Senior Staffing Specialist Platinum Select Staffing 866-217-3481 866-217-1742 fax Apply Now: www.platinumselect.org A Division of AMN Healthcare Platinum Select, When Personal Service is Everything Platinum Select is certified by the Joint Commission (JCAHO) CONFIDENTIALITY NOTICE This message and any included attachments are from Platinum Select Helthcare Staffing, Inc. and are intended only for the addressee. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws and is intended only for the use of the addressee. Unauthorized forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Platinum Select's corporate office located at 325 N. St. Paul, Ste. 4200, Dallas, Texas, U.S.A. - (+1) (866) 953-0011. From tkngflght <@t> yahoo.com Thu Oct 2 11:59:40 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Oct 2 11:59:50 2008 Subject: [Histonet] Seeking histologists in Houston and a correction for the PAs on the list! Message-ID: Hi everyone-me again. And again we need a couple of talented histotechs for local temporary work....walk in and work routine histology, get paid pretty well and very very appreciated by the folks who need your support! Two openings, day shift, starting ASAP for embed cut stain and a little more. These could work with an afternoon or night tech looking to pay off some bills or try something new without leaving their current job. Confidential inquiries welcome!! You'll talk with me directly so you can relax and pick up the phone--it's all about creating situations that work and everyone wins!! The second is my apology regarding the post seeking a PA in Ohio: PAs are Pathologists' Assistants. I didn't have the correct title and with the work you undertake to become a PA, getting that right is a mark of respect for your position. I'm sorry and I won't post it incorrectly again!! Thanks to everyone for your continued support--Histotechs & Pathologists' Assistants are a cut above!! Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org From Simoskevitz <@t> Osteotech.com Thu Oct 2 12:30:26 2008 From: Simoskevitz <@t> Osteotech.com (Simoskevitz@Osteotech.com) Date: Thu Oct 2 12:30:12 2008 Subject: [Histonet] Alkaline Phosphatase Stain In-Reply-To: <20081002170103.94777A0D9A@l3inpf4.contentcatcher.com> Message-ID: Does any have a good alk phos stain for glycol methacrylate sections? Any help would be greatly appreciated. Thanks, Ricki ********************************************* Ricki Simoskevitz, Research Associate III Osteotech Inc. 51 James Way Eatontown, NJ 07724 Phone: (732) 542-2800 X6328 Fax: (732) 935-1298 ********************************************** From integrated.histo <@t> gmail.com Thu Oct 2 12:43:03 2008 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Thu Oct 2 12:43:07 2008 Subject: [Histonet] Alcian Green Message-ID: <5d9104a30810021043j6d376246r29edb6ddcd422e3a@mail.gmail.com> One of my pathologists asked us if we could perform an Alcian Green stain (for Helicobacter pylori). I had not heard of it so a quick search on histonet gave a few hits in 2005, mainly about that it is no longer available. Is anyone currently doing such a stain? Cindy DuBois From integrated.histo <@t> gmail.com Thu Oct 2 13:13:12 2008 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Thu Oct 2 13:13:16 2008 Subject: [Histonet] Alcian Yellow Message-ID: <5d9104a30810021113q6688c571s51d568ba1c69fd9c@mail.gmail.com> Does anyone have an Alcian Yellow procedure they are willing to share? It is for staining H. pylori. Cindy Dubois From llewllew <@t> shaw.ca Thu Oct 2 13:49:15 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Oct 2 13:49:29 2008 Subject: [Histonet] Winterize formalin? References: <8854ff80810020758p488d4c1bpa88cb7edae120968@mail.gmail.com> Message-ID: <8A0979A8783A4572BD461B7C30C63A36@Compaq> When I was in Prince George, BC we used to tell our satellite labs to dilute the NBF 20% with ethanol . This worked fine for us and below -20C. At really cold temperatures it turned slushy rather than solid, but we rarely got ice crystal holes. I used to live and work in Winnipeg. I was the section head in histology at the HSC from 67-74. How are things there, now? Bryan Llewellyn ----- Original Message ----- From: "Sate Hamza" To: Sent: Thursday, October 02, 2008 7:58 AM Subject: [Histonet] Winterize formalin? > Dear Histonetters, > > A colleague from Boston posted this inquiry to our dermatopathology > mailing > list: "Does anyone [have] the formula to winterize formalin? We were > adding > 15 ml of alcohol to 1000 ml of 10% buffered formalin. It used to work but > is > not working now at -20" .. > > Interestingly, I found out that we do not "winterize" formalin up here in > Winnipeg, Canada (also jokingly referred to as "Winterpeg"). I am > wondering > if anyone on the list "winterizes" formalin .. I found the following > abstract of a Japanese paper by searching in Pubmed > > http://www.ncbi.nlm.nih.gov/pubmed/3692431 > > Thank you .. > > Sate > > -- > Sate Hamza, MD, FRCPC > Dermatopathologist > Winnipeg, Canada > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From denise.woodward <@t> uconn.edu Thu Oct 2 13:57:49 2008 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Thu Oct 2 13:57:53 2008 Subject: [Histonet] Histology or IHC conferences/workshops In-Reply-To: <35e16a770809302036g510058e3v9afeb5ee3680012d@mail.gmail.com> References: <35e16a770809302036g510058e3v9afeb5ee3680012d@mail.gmail.com> Message-ID: <40AC6D73C2B95C4CA21B26B7BF380C4002D0E484@EXCHANGED.mgmt.ad.uconn.edu> The next Region 1 Symposium will be held in Newport Rhode Island, April 16- 18 2009. for more information see www.RIhistology.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Igor Deyneko Sent: Tuesday, September 30, 2008 11:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology or IHC conferences/workshops Dear Histonetters! I was wondering where someone knows of any useful conferences or workshops , which deal with histological techniques, or Immunohistochemical techniques or even microscopy techniques( I heard about a place in Cape Cod), coming up in 2009??? Any information would be appreciated. Thank you. Igor Deyneko Infinity Pharmaceuticals In-Vivo Pharmacology Cambridge, MA 02139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Thu Oct 2 14:47:21 2008 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Thu Oct 2 14:47:25 2008 Subject: [Histonet] Histology Technician Opening in Charleston, SC Message-ID: <817C2761C5A1394180709AEEDB775B7E06A44E2A@NASEV03.hca.corpad.net> Hello to All, Do you love going to the beach? Or history? Or... NO SNOW??? Trident Medical Center in Charleston, SC has an opening for a histology technician HT(ASCP) registered required. Routine embedding, microtomy, frozen sections, H&E and Special Stains on approximately 12,000 cases per year. IHC staining performed by senior histotech. Modern up-to-date equipment in a nice roomy five year old lab. Friendly staff and great Pathologist. For more information and an application go to www.tridenthealthsystem.com WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax From pruegg <@t> ihctech.net Thu Oct 2 15:27:47 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Oct 2 15:27:50 2008 Subject: [Histonet] Stem Cell Factor Message-ID: <08C5F5963B61474E8AEA9473F80E2D64@Patsyoffice> Does anyone have IHC experience with Stem Cell Factor Ab on ffpe skin samples or any ffpe samples? Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From lpwenk <@t> sbcglobal.net Thu Oct 2 20:33:30 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Oct 2 20:33:45 2008 Subject: [Histonet] Alcian Yellow In-Reply-To: <5d9104a30810021113q6688c571s51d568ba1c69fd9c@mail.gmail.com> Message-ID: <000001c924f8$0b62bf40$0202a8c0@HPPav2> Alcian Yellow is no longer being made. Since Alcian Green (your previous email) is a mixture of Alcian Yellow and Alcian Blue, it no longer is being made. That said, ANATECH has an alternative yellow dye that stains mucin, called HP Yellow (HP for Helicobacter pylorii). You can contact them at 800-ANATECH. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Thursday, October 02, 2008 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alcian Yellow Does anyone have an Alcian Yellow procedure they are willing to share? It is for staining H. pylori. Cindy Dubois _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MichelM9 <@t> chw.edu.au Thu Oct 2 21:17:01 2008 From: MichelM9 <@t> chw.edu.au (Michelle McDonald) Date: Thu Oct 2 21:17:15 2008 Subject: [Histonet] Beta-Gal antibody In-Reply-To: Message-ID: <47BD6E7614A693499453835D47E36F7004EA93D7@hedwig.nch.kids> We use one we get from Sapphire Bioscience, catalogue number AB616 for $497 AUD. Michelle Dr Michelle McDonald B.MedSci, PhD Research Officer Orthopaedic Research Dept. The Children's Hospital Westmead. Westmead NSW 2145 Australia Ph. +612 9845 1451 Fax. +612 9845 3078 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dustin Hambright Sent: Tuesday, 23 September 2008 1:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Beta-Gal antibody Hello, Does anyone know a reliable source for a Beta-Gal (lac-z) antibody for mouse frozen immunohistochemistry? Do you know the company and dilution? Thank you in advance, Dustin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From pieronelva01 <@t> bigpond.com Fri Oct 3 04:16:01 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Fri Oct 3 04:16:14 2008 Subject: [Histonet] Does anyone have any ideas on the use of softener on decal blocks. Does this help 'soften' them? If so how? References: <48E4EA0E020000B500002A23@ekhgwia.ekht.nhs.uk> Message-ID: <2EBB4E375CB948FA81930B5C49BC9F6F@pentium4> Hi Sarah I don't use a softener as such, but have had good results putting trimmed blocks on a water soaked tissue on the bench for 30 min or so just before cooling and sectioning. I find it is more effective than an ice bath at softening tissues. REgards Piero Nelva Anatomical Pathology Monash Medical Centre Australia ----- Original Message ----- From: "SARAH REEVES" To: Sent: Friday, October 03, 2008 12:34 AM Subject: [Histonet] Does anyone have any ideas on the use of softener on decal blocks. Does this help 'soften' them? If so how? Does anyone have any ideas on the use of softener on decal blocks. Does this help 'soften' them? If so how? Thanks in advance Sarah ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals University NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.7.5/1704 - Release Date: 10/2/2008 9:35 PM From amosbrooks <@t> gmail.com Fri Oct 3 07:31:38 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Oct 3 07:31:44 2008 Subject: [Histonet] Isopropyl vs Ethanol Message-ID: <582736990810030531i1258ef03hd334e15004bccbc@mail.gmail.com> Hi, Is there anyone who uses exclusively isopropyl alcohol to process with rather than ethanol? I have had great results with isopropyl alcohol for some tissues, but I wanted to know if there are any pitfalls to using it exclusively for processing. I've noticed that isopropyl is much cheaper than ethanol, so it leads me to wonder why ethanol is so prevalant for dehydration. Thanks, Amos Brooks From gschaaf <@t> hotmail.com Fri Oct 3 07:44:51 2008 From: gschaaf <@t> hotmail.com (Gerben Schaaf) Date: Fri Oct 3 07:44:55 2008 Subject: [Histonet] damage to freeze-sections after cutting In-Reply-To: References: Message-ID: Dear histonet users, I am just starting to do cryosections and I ran into a problem. I have mouse tissue that we freeze in OCT (we tried different tissue preprocessing: PFA immersion fix, whole animal PFA perfusion, sucrose gradient). I am sectioning skeletal muscle at 10 micron. When I look at the sections directly after sectioning (slide is still cold), the morphology looks ok. However, if I allow the slide to warm to room temp than the tissue start to 'tear' apart and the individual fibers get separated. I could keep the sections at -20C and they will be ok, however, during staining the slides will warm to RT and the above problem occurs. What can I do to prevent the tissue from starting to tear? Thanks, Gerben, Rotterdam, The Netherlands _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/ From rjbuesa <@t> yahoo.com Fri Oct 3 08:04:47 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 3 08:04:53 2008 Subject: [Histonet] Isopropyl vs Ethanol In-Reply-To: <582736990810030531i1258ef03hd334e15004bccbc@mail.gmail.com> Message-ID: <68888.56661.qm@web65713.mail.ac4.yahoo.com> Amos: Isopropyl alcohol (IPA) penetrates less quickly than ethanol, but dehydrates more gently and hardens the tissues less. When the tissue ihas been dehydrated with IPA, you can mixe it in a proportion of 5:1 and 2:1 with mineral oil at 50?C and by doing so you can ELIMINATE --- On Fri, 10/3/08, Amos Brooks wrote: From: Amos Brooks Subject: [Histonet] Isopropyl vs Ethanol To: "histonet@lists.utsouthwestern.edu" Date: Friday, October 3, 2008, 8:31 AM Hi, Is there anyone who uses exclusively isopropyl alcohol to process with rather than ethanol? I have had great results with isopropyl alcohol for some tissues, but I wanted to know if there are any pitfalls to using it exclusively for processing. I've noticed that isopropyl is much cheaper than ethanol, so it leads me to wonder why ethanol is so prevalant for dehydration. Thanks, Amos Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Oct 3 08:07:28 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 3 08:07:33 2008 Subject: [Histonet] Isopropyl vs Ethanol In-Reply-To: <582736990810030531i1258ef03hd334e15004bccbc@mail.gmail.com> Message-ID: <5981.20125.qm@web65705.mail.ac4.yahoo.com> Amos: Isopropyl alcohol (IPA) penetrates less quickly than ethanol, but dehydrates more gently and hardens the tissues less. When the tissue has been dehydrated with IPA, you can mix it in a proportion of 5:1?(followed by another at?2:1) with mineral oil at 50?C and by doing so you can ELIMINATE using xylene from tissue processing, something you cannot do with ethanol. Ren? J. --- On Fri, 10/3/08, Amos Brooks wrote: From: Amos Brooks Subject: [Histonet] Isopropyl vs Ethanol To: "histonet@lists.utsouthwestern.edu" Date: Friday, October 3, 2008, 8:31 AM Hi, Is there anyone who uses exclusively isopropyl alcohol to process with rather than ethanol? I have had great results with isopropyl alcohol for some tissues, but I wanted to know if there are any pitfalls to using it exclusively for processing. I've noticed that isopropyl is much cheaper than ethanol, so it leads me to wonder why ethanol is so prevalant for dehydration. Thanks, Amos Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dermpathsy <@t> gmail.com Fri Oct 3 10:22:04 2008 From: dermpathsy <@t> gmail.com (Sate Hamza) Date: Fri Oct 3 10:22:09 2008 Subject: [Histonet] Winterize formalin? In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F546@TRFT-EX01.xRothGen.nhs.uk> References: <5C0BED61F529364E86309CADEA63FEF20163F546@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <8854ff80810030822m44f16970r910d3e76a24a2bcb@mail.gmail.com> Thank you Terry .. and thanks to everyone who replied to me about this ... Sate On Thu, Oct 2, 2008 at 10:37 AM, Marshall Terry Dr, Consultant Histopathologist wrote: > 1.5% sounds homeopathic to me. > > There will be more depression of freezing point due to the salts in > buffered formalin than anything. > > In the excerpt you gave Sate, antifreeze worked best (surprise > surprise). > > It's hard to imagine with modern transport that things are going to hang > around for long enough and at such a low temperature that crystals are > going to form. > > Terry > > PS I had a picture of the freezing artefact once but have lost it. If > someone has one I sure would appreciate it. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sate > Hamza > Sent: 02 October 2008 15:58 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Winterize formalin? > > Dear Histonetters, > > A colleague from Boston posted this inquiry to our dermatopathology > mailing > list: "Does anyone [have] the formula to winterize formalin? We were > adding > 15 ml of alcohol to 1000 ml of 10% buffered formalin. It used to work > but is not working now at -20" .. > > Interestingly, I found out that we do not "winterize" formalin up here > in Winnipeg, Canada (also jokingly referred to as "Winterpeg"). I am > wondering if anyone on the list "winterizes" formalin .. I found the > following abstract of a Japanese paper by searching in Pubmed > > http://www.ncbi.nlm.nih.gov/pubmed/3692431 > > Thank you .. > > -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada From pattennj <@t> mail.nih.gov Fri Oct 3 12:27:56 2008 From: pattennj <@t> mail.nih.gov (Patten, Nicole (NIH/NIAAA) [F]) Date: Fri Oct 3 12:28:00 2008 Subject: [Histonet] Antigen Retrieval Message-ID: Hi- I am new to using IHC and I need some advice on Antigen Retrieval. I usually do it in an autoclave for 15min at 121C for FFPE human brain tissue (in Citrate or Tris Buffer), but by the end my tissue looks pretty bad and parts have even fallen off. The tissue is fixed on SuperFrost Plus Slides. Any suggestions would be really helpful. Thanks! N. Patten Post-Baccalaureate Fellow/IRTA National Institutes of Health From LSebree <@t> uwhealth.org Fri Oct 3 12:48:53 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Fri Oct 3 12:48:57 2008 Subject: [Histonet] Antigen Retrieval In-Reply-To: Message-ID: Try using a laboratory pressure cooker like the Decloaking Chamber from Biocare Medical. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patten, Nicole (NIH/NIAAA) [F] Sent: Friday, October 03, 2008 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen Retrieval Hi- I am new to using IHC and I need some advice on Antigen Retrieval. I usually do it in an autoclave for 15min at 121C for FFPE human brain tissue (in Citrate or Tris Buffer), but by the end my tissue looks pretty bad and parts have even fallen off. The tissue is fixed on SuperFrost Plus Slides. Any suggestions would be really helpful. Thanks! N. Patten Post-Baccalaureate Fellow/IRTA National Institutes of Health _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Fri Oct 3 12:48:52 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Fri Oct 3 12:51:38 2008 Subject: [Histonet] Isopropyl vs Ethanol Message-ID: <264159701.20081003214852@mail.ru> Amos: One disadvantage for manual processing with IPA is a little odor of "cat's urine". This is can fully eliminated when using VIP processor. We changed ethanol on IPA more than years ago and happy with it. We also uses mineral oil instead xylene and also happy. However none of our techs want not return to etanol, xylene and other processing reagents. If you want, please contact with me for details. Rene very right. Sincerely, Maxim Peshkov Russia, Taganrog. > --- On Fri, 10/3/08, Amos Brooks > wrote: > > From: Amos Brooks > Subject: [Histonet] Isopropyl vs Ethanol > To: "histonet@lists.utsouthwestern.edu" > > Date: Friday, October 3, 2008, 8:31 AM > > Hi, > Is there anyone who uses exclusively isopropyl > alcohol to process with > rather than ethanol? I have had great results with > isopropyl alcohol for > some tissues, but I wanted to know if there are any > pitfalls to using it > exclusively for processing. I've noticed that > isopropyl is much cheaper > than > ethanol, so it leads me to wonder why ethanol is so > prevalant for > dehydration. > > Thanks, > Amos Brooks From CHRISH <@t> HEALTHCARESCOUTS.COM Fri Oct 3 14:08:49 2008 From: CHRISH <@t> HEALTHCARESCOUTS.COM (Chris Handrahan) Date: Fri Oct 3 14:15:52 2008 Subject: [Histonet] Hot Histology Openings TX, FL, PA, VA, KS, IN, WA Message-ID: Full time permanent positions available in Dallas, TX San Antonio, TX Tampa, FL Orlando, FL Pittsburgh, PA Richmond, VA Overland Park, KS Fort Wayne, IN Seattle, WA Please contact me today if you are interested! Healthcare Scouts is a medical laboratory/biotech recruitment firm that places professionals in full-time, permanent positions throughout the United States. Please visit www.healthcarescouts.com Chris Handrahan Managing Director of Allied Health Healthcare Scouts 800-708-0605 office 321-231-5427 cell chrish@healthcarescouts.com www.healthcarescouts.com From sccrshlly <@t> yahoo.com Fri Oct 3 18:07:33 2008 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Fri Oct 3 18:07:36 2008 Subject: [Histonet] Re: Alcian Yellow Message-ID: <918977.28334.qm@web90304.mail.mud.yahoo.com> American Mastertech has a Helico*STAT stain kit that uses Alcian yellow solution and toluidine blue as staining components. If this is similar to what you are currently using, I am sure they would share their procedure with you if you called. I saw this stain at the Texas state meeting, and the organisms stand out very nicely! We currently use the Thiazine stain in the Diff Quick kit for our pathologists. Michelle Coker > > Does anyone have an Alcian Yellow procedure they are > willing to share? It > is for staining H. pylori. > > Cindy Dubois From laurie.reilly <@t> jcu.edu.au Fri Oct 3 18:57:48 2008 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Fri Oct 3 18:58:18 2008 Subject: [Histonet] Does anyone have any ideas on the use of softener ondecal blocks. Does this help 'soften' them? If so how? In-Reply-To: <2EBB4E375CB948FA81930B5C49BC9F6F@pentium4> References: <48E4EA0E020000B500002A23@ekhgwia.ekht.nhs.uk> <2EBB4E375CB948FA81930B5C49BC9F6F@pentium4> Message-ID: <74B6791EBFBF420A8E00F77D55FE7FFE@health.ad.jcu.edu.au> Floating the trimmed/faced block on the waterbath for 2 minutes helps. Then put them on wet ice to cool. Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piero Nelva Sent: Friday, 3 October 2008 7:16 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Does anyone have any ideas on the use of softener ondecal blocks. Does this help 'soften' them? If so how? Hi Sarah I don't use a softener as such, but have had good results putting trimmed blocks on a water soaked tissue on the bench for 30 min or so just before cooling and sectioning. I find it is more effective than an ice bath at softening tissues. REgards Piero Nelva Anatomical Pathology Monash Medical Centre Australia ----- Original Message ----- From: "SARAH REEVES" To: Sent: Friday, October 03, 2008 12:34 AM Subject: [Histonet] Does anyone have any ideas on the use of softener on decal blocks. Does this help 'soften' them? If so how? Does anyone have any ideas on the use of softener on decal blocks. Does this help 'soften' them? If so how? Thanks in advance Sarah ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals University NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- ---- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.7.5/1704 - Release Date: 10/2/2008 9:35 PM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Oct 4 10:49:49 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Oct 4 10:49:38 2008 Subject: [Histonet] Antigen Retrieval In-Reply-To: Message-ID: Nicole, You might look into using a more gentle heat source, such as a vegetable steamer or water bath. There are also pressure cookers for IHC which may also be easier on your tissue that the autoclave, although I have had problems with them as well and stick to steamer or waterbath myself. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patten, Nicole (NIH/NIAAA) [F] Sent: Friday, October 03, 2008 11:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen Retrieval Hi- I am new to using IHC and I need some advice on Antigen Retrieval. I usually do it in an autoclave for 15min at 121C for FFPE human brain tissue (in Citrate or Tris Buffer), but by the end my tissue looks pretty bad and parts have even fallen off. The tissue is fixed on SuperFrost Plus Slides. Any suggestions would be really helpful. Thanks! N. Patten Post-Baccalaureate Fellow/IRTA National Institutes of Health _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sat Oct 4 12:47:43 2008 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Oct 4 12:48:04 2008 Subject: [Histonet] re: Isopropyl vs Ethanol Message-ID: <11D9615B89C10747B1C985966A63D7CA286308763F@KCL-MAIL04.kclad.ds.kcl.ac.uk> I never use ethanol for any histological-based system. I use IMS (Industrial methylated spirit/ Industrial Denatured Alcohol -IDA ) Is IMS less/more expensive than IPA? I use IMS for pwax processing/rehydrating/dehydrating my pwax sections. ( us Brits will most likely use 74OP = ~99%: VWR list price is ?135 for 25 L. However, every large Organisation buys it considerably cheaper.) Rene's suggestion to use IPA for pwax processing is a very good alternative: you do not need to use a "clearing " agent, like xylene. (Of course, your tissues will not "clear", lol: in the old days it was an indicator that the tissue was ready to move on to the next stage....these days we just rely on Protocols ;-) In my experience, IPA does not dissolve paraffin wax at RT BUT, at the 60C temp. of wax in a tissue processor/wax oven if processing by hand, IPA and wax are entirely miscible. From ccrowder <@t> vetmed.lsu.edu Sat Oct 4 18:55:51 2008 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Sat Oct 4 18:58:23 2008 Subject: [Histonet] softening decals Message-ID: We use Downy on bones which have not been fully decaled. We face the block, then put soak it in Downy for 5-10 minutes, even longer sometimes. We cut the block last. We seem to be able to get a good section from the first and second cuts. The only problem we have had is that the first "scent" of the softener was too sweet and we hated smelling it. We chose another scent for the next container. I have no idea why it works, but it does. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From Maxim_71 <@t> mail.ru Sun Oct 5 13:12:54 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Sun Oct 5 13:18:14 2008 Subject: [Histonet] re: Isopropyl vs Ethanol Message-ID: <1186719388.20081005221254@mail.ru> Carl: In mineral oil all tissues will "clear" as in old days, when we used xylene. This is good test for adequate dehydration and clearing I uses now. Mineral oil very well mixes as with IPA at 50oC, as with paraffin at 60oC or so. Regards, Maxim Peshkov Russia, Taganrog. Carl Hobbs wrote: > I never use ethanol for any histological-based > system. > I use IMS (Industrial methylated spirit/ Industrial > Denatured Alcohol -IDA ) > Is IMS less/more expensive than IPA? > I use IMS for pwax > processing/rehydrating/dehydrating my pwax sections. > ( us Brits will most likely use 74OP = ~99%: VWR > list price is ?135 for 25 L. However, every large > Organisation buys it considerably cheaper.) > > Rene's suggestion to use IPA for pwax processing is > a very good alternative: you do not need to use a > "clearing " agent, like xylene. > (Of course, your tissues will not "clear", lol: in > the old days it was an indicator that the tissue was > ready to move on to the next stage....these days we > just rely on Protocols ;-) > > In my experience, IPA does not dissolve paraffin wax > at RT BUT, at the 60C temp. of wax in a tissue > processor/wax oven if processing by hand, IPA and wax > are entirely miscible. mailto:Maxim_71@mail.ru From amosbrooks <@t> gmail.com Sun Oct 5 20:05:09 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Oct 5 20:05:14 2008 Subject: [Histonet] ETOH vs IPA Message-ID: <582736990810051805q3540a8a5g796ceb1c58714760@mail.gmail.com> Hi, Thanks for all the suggestions for using IPA in place of ETOH. I will probably try running a few blocks on the processor to see how it goes. It's always good to hear from folks to know of potential pitfalls before trying this out. Hopefully it will improve the difficult mouse tissue. Thanks, Amos From paintedsplashes <@t> yahoo.com Mon Oct 6 05:02:22 2008 From: paintedsplashes <@t> yahoo.com (Jeanne Clark) Date: Mon Oct 6 05:02:29 2008 Subject: [Histonet] job opening coming up Message-ID: <802219.94703.qm@web30708.mail.mud.yahoo.com> A full time HT position is coming available at Mission Hospitals in beautiful Asheville, NC. Contact me for more information! ? Jeanne Jeanne Clark HT/MLT (ASCP) Pathology Manager Mission Hospitals 428 Biltmore Ave Asheville, NC 28801 828-213-5169 office 423-612-1213 cell ? ? From SwainFrancesL <@t> uams.edu Mon Oct 6 06:38:20 2008 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Mon Oct 6 06:40:01 2008 Subject: [Histonet] Antigen Retrieval In-Reply-To: References: Message-ID: I work with non-decalcified and decalcified bone. After I have tried to pressure cook it, steam it, microwave it, etc my sections look pretty bad(coming off, etc.) I have found that either using Pepsin (Zymed) for 20 minutes at 37 degrees C. in a humidifying chamber or Pepsin for 20 minutes at room temperature in a humidifying chamber or microwave the buffer to boiling, quickly remove and place slides in solution, cover and let sit for 20 minutes or until cool to touch work the best for my purposes. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Friday, October 03, 2008 12:49 PM To: Patten, Nicole (NIH/NIAAA) [F]; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Antigen Retrieval Try using a laboratory pressure cooker like the Decloaking Chamber from Biocare Medical. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patten, Nicole (NIH/NIAAA) [F] Sent: Friday, October 03, 2008 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antigen Retrieval Hi- I am new to using IHC and I need some advice on Antigen Retrieval. I usually do it in an autoclave for 15min at 121C for FFPE human brain tissue (in Citrate or Tris Buffer), but by the end my tissue looks pretty bad and parts have even fallen off. The tissue is fixed on SuperFrost Plus Slides. Any suggestions would be really helpful. Thanks! N. Patten Post-Baccalaureate Fellow/IRTA National Institutes of Health _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Susan.Walzer <@t> HCAHealthcare.com Mon Oct 6 06:44:13 2008 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Mon Oct 6 06:44:22 2008 Subject: [Histonet] Isopropyl vs Ethanol In-Reply-To: <582736990810030531i1258ef03hd334e15004bccbc@mail.gmail.com> References: <582736990810030531i1258ef03hd334e15004bccbc@mail.gmail.com> Message-ID: <471953BC63077941B82C26A4338272B42F061A@ORLEV03.hca.corpad.net> We don't use it for processing but we use it at the end of the stain set up and coverslip out of it using Clearium mounting medium (it is misable with alcohol). We did it to keep out fingers out of xylene as it is a neurotoxin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, October 03, 2008 8:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Isopropyl vs Ethanol Hi, Is there anyone who uses exclusively isopropyl alcohol to process with rather than ethanol? I have had great results with isopropyl alcohol for some tissues, but I wanted to know if there are any pitfalls to using it exclusively for processing. I've noticed that isopropyl is much cheaper than ethanol, so it leads me to wonder why ethanol is so prevalant for dehydration. Thanks, Amos Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Mon Oct 6 07:28:22 2008 From: tifei <@t> foxmail.com (tf) Date: Mon Oct 6 08:02:00 2008 Subject: [Histonet] Antigen Retrieval References: , , Message-ID: <200810062028171173275@foxmail.com> SGksIGFkanVzdCB0aGUgZ2VsYXRpbiBjb25jZW50cmF0aW9uIQ0KDQpPdXIgZXhwZXJpZW5jZXMg b2YgYnJhaW4gc2VjdGlvbiAoOTUgZGVncmVlIGJvaWxpbmcgZm9yIDMwIG1pbik6IDAuNSUgZ2Vs YXRpbi1jb2F0ZWQgc2xpZGVzIGNvbWluZyBvZmYgc2VjdGlvbnMsIG5vdCBmb3IgMC4yNSUuDQpB bHNvLCB0cnkgdG8gdXNlICJmbG9hdGluZyBzZWN0aW9ucyIgcmF0aGVyIGRpcmVjdCBhdHRhY2gg ZnJvemVuIHNlY3Rpb25zIGVtYmVkZGVkIHdpdGggTy5DLlQgb24gdG8gc2xpZGVzLg0KDQoNCjIw MDgtMTAtMDYgDQoNCg0KDQp0ZiANCg0KDQoNCreivP7Iy6O6IFN3YWluLCBGcmFuY2VzIEwgDQq3 osvNyrG85KO6IDIwMDgtMTAtMDYgIDE5OjQ0OjM4IA0KytW8/sjLo7ogU2VicmVlIExpbmRhIEEu 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X19fX19fX19fX19fX19fX19fX19fX19fX19fDQpIaXN0b25ldCBtYWlsaW5nIGxpc3QNCkhpc3Rv bmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdQ0KaHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJu LmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0DQpfX19fX19fX19fX19fX19fX19fX19fX19f X19fX19fX19fX19fX19fX19fX19fXw0KSGlzdG9uZXQgbWFpbGluZyBsaXN0DQpIaXN0b25ldEBs aXN0cy51dHNvdXRod2VzdGVybi5lZHUNCmh0dHA6Ly9saXN0cy51dHNvdXRod2VzdGVybi5lZHUv bWFpbG1hbi9saXN0aW5mby9oaXN0b25ldA0KQ29uZmlkZW50aWFsaXR5IE5vdGljZTogVGhpcyBl LW1haWwgbWVzc2FnZSwgaW5jbHVkaW5nIGFueSBhdHRhY2htZW50cywgaXMgZm9yIHRoZSBzb2xl IHVzZSBvZiB0aGUgaW50ZW5kZWQgcmVjaXBpZW50KHMpIGFuZCBtYXkgY29udGFpbiBjb25maWRl bnRpYWwgYW5kIHByaXZpbGVnZWQgaW5mb3JtYXRpb24uICBBbnkgdW5hdXRob3JpemVkIHJldmll dywgdXNlLCBkaXNjbG9zdXJlIG9yIGRpc3RyaWJ1dGlvbiBpcyBwcm9oaWJpdGVkLiAgSWYgeW91 IGFyZSBub3QgdGhlIGludGVuZGVkIHJlY2lwaWVudCwgcGxlYXNlIGNvbnRhY3QgdGhlIHNlbmRl ciBieSByZXBseSBlLW1haWwgYW5kIGRlc3Ryb3kgYWxsIGNvcGllcyBvZiB0aGUgb3JpZ2luYWwg bWVzc2FnZS4NCl9fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19f DQpIaXN0b25ldCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVk dQ0K From Dorothy.L.Webb <@t> HealthPartners.Com Mon Oct 6 09:16:00 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Oct 6 09:16:05 2008 Subject: [Histonet] validation Message-ID: <0E394B648E5284478A6CCB78E5AFDA27056359F2@hpes1.HealthPartners.int> Is it better to use a lab's own tissue for validation in a microarray for Ihc purposes or would it be passable to use TMA's from another source?? Thanks for your opinions!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From rjbuesa <@t> yahoo.com Mon Oct 6 09:22:22 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 6 09:22:31 2008 Subject: [Histonet] validation In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA27056359F2@hpes1.HealthPartners.int> Message-ID: <884936.89192.qm@web65705.mail.ac4.yahoo.com> Dorothy: At the end you are going to use tissues from your laboratory to do the tests, and you do not know how other labs processed their tissues. Although it is not absolutely clear how the processing steps (other than fixation) can affect the reactivity of a tissue, it is always better not to introduce more variables than necessary, so I would use tissues from my own laboratory. Ren? J. --- On Mon, 10/6/08, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] validation To: histonet@lists.utsouthwestern.edu Date: Monday, October 6, 2008, 10:16 AM Is it better to use a lab's own tissue for validation in a microarray for Ihc purposes or would it be passable to use TMA's from another source?? Thanks for your opinions!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Mon Oct 6 10:01:36 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Oct 6 10:01:40 2008 Subject: [Histonet] Sulfur Stain for Brains? Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E64E0@nmdamailsvr.nmda.ad.nmsu.edu> One of our students is interested in staining sheep brains for sulfur - is there a definitive stain for this? I've done some basic searching but come up empty. Thanks. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From eca9 <@t> georgetown.edu Mon Oct 6 10:04:33 2008 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Mon Oct 6 10:04:38 2008 Subject: [Histonet] gastrin antibodies..... Message-ID: <48EA2901.8030804@georgetown.edu> Good morning out there in histoland, I am looking for some help in finding two antibodies: - sulfated gastrin-17 (G-7) - sulfated cholecystokinin-8 (CCK-8) All the antibodies that I are finding are non-sulfated. Thank you all for your help, Eva Permaul Georgetown University From baza0013 <@t> d.umn.edu Mon Oct 6 10:27:37 2008 From: baza0013 <@t> d.umn.edu (Adam Bazama) Date: Mon Oct 6 10:27:35 2008 Subject: [Histonet] sirius red connective tissue stain[Scanned] Message-ID: <000201c927c8$10a68220$31f38660$@umn.edu> Hi Dave, I noticed that you posted a request in march of 2004 on histonet for a Sirius red fast green staining protocol. If you do have a protocol, would you mind sending it to me. I have had a hard time finding one to compare to mine and my lack luster results. Thank you, Adam Bazama, B.S. Junior Scientist Lillehei Heart Institute Histology and Microscopy Core Facility University of Minnesota Medical School, Division of Cardiology 4-266 Nils Hasselmo Hall 312 Church Street SE Minneapolis, MN 55455 Lab Desk: 612-625-6779 Cell: 952-334-0607 From azdudley <@t> hotmail.com Mon Oct 6 11:19:13 2008 From: azdudley <@t> hotmail.com (anita dudley) Date: Mon Oct 6 11:19:21 2008 Subject: [Histonet] out dated reagents Message-ID: hello all, we were inspected the beginning of june by cap and one of our deficiency was anp.21382. it had to do with outdated reagents. my director want all of my dry chemicals gone!!!! I have gone through them and just have what I need for the special stains that we do in house. does anyone know do they put expiration dates on dry chems. we have things like light green, biebrich scarlet, periodic acid. I really hate to get rid of all of this but he thinks I should. what are others doing about this? I can see after you have made up things to not keep it long but dry I know many yrs ago the biological stain commission thought it would be meaningless and arbitrary to put expiration dates on stains. it would me more important to maintain a record of the use of the powdered stain. which is what we do when we do the stain with our controls. anyway just wondering if there were any thoughts out there about this. anita dudley providence hosp mobile alabama _________________________________________________________________ See how Windows Mobile brings your life together?at home, work, or on the go. http://clk.atdmt.com/MRT/go/msnnkwxp1020093182mrt/direct/01/ From mikael.niku <@t> helsinki.fi Mon Oct 6 11:34:37 2008 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Mon Oct 6 11:34:41 2008 Subject: [Histonet] RNALater, cryosections, OCT, and RNA isolation In-Reply-To: <48AD705E.4000706@umdnj.edu> References: <001301c90261$093fcff0$be00000a@CPU360> <006e01c9036a$e2abb3d0$97a5d680@mmkem12636> <48AD705E.4000706@umdnj.edu> Message-ID: <48EA3E1D.3070305@helsinki.fi> Dear Histonetters, I'm looking for methods to preserve intestinal tissue samples, in order to later isolate RNA from laser microdissected cryosections. I'm a bit worried about the intestinal enzymes (not sure if the RNA will be preserved even at -80C for very long). I was told that RNALater might be better, but I don't have personal experience on the stuff. Does anybody know if RNALater-treated tissues can be later frozen and cut to adequate cryosections? How about antigenicity, as we would probably like to use the same samples for immunohistochemistry as well? Another question: is it true that OCT-embedded frozen tissues can be used to isolate RNA (with Trizol, for example)? I know these have been discussed in the past, but couldn't find definite answers in the archives. Perhaps more experience has been gathered since then! With best regards, Mikael Niku ----------------------------------------------------- Mikael Niku, PhD, university lecturer University of Helsinki, Division of Nutrition URL: mikael.nikunnakki.info - What do I think of western civilization? I think it would be a good idea! (Gandhi) ----------------------------------------------------- From JCollins <@t> palmbeachpath.com Mon Oct 6 11:44:20 2008 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Mon Oct 6 11:44:24 2008 Subject: [Histonet] out dated reagents In-Reply-To: References: Message-ID: <05CAE76AB5D5ED409864C6DD86F133490240DD02C0@pbpsflexch02.pbp.local> We created an access database file (you could also do it on an excel spreadsheet) with all the dry chemicals. We included date received, date opened, chemical catalog #, name and expiration date. We assigned expiration dates based on our best knowledge of how long it would be good. We then sorted the file by expiration date so we can keep track of chemicals which are due to expire and printed it out. When the expiration date comes up we check the chemical and either discard it or adjust the expiration date. This way we can keep track of them all easily. Judy Collins Palm Beach Pathology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Monday, October 06, 2008 12:19 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] out dated reagents hello all, we were inspected the beginning of june by cap and one of our deficiency was anp.21382. it had to do with outdated reagents. my director want all of my dry chemicals gone!!!! I have gone through them and just have what I need for the special stains that we do in house. does anyone know do they put expiration dates on dry chems. we have things like light green, biebrich scarlet, periodic acid. I really hate to get rid of all of this but he thinks I should. what are others doing about this? I can see after you have made up things to not keep it long but dry I know many yrs ago the biological stain commission thought it would be meaningless and arbitrary to put expiration dates on stains. it would me more important to maintain a record of the use of the powdered stain. which is what we do when we do the stain with our controls. anyway just wondering if there were any thoughts out there about this. anita dudley providence hosp mobile alabama _________________________________________________________________ See how Windows Mobile brings your life together-at home, work, or on the go. http://clk.atdmt.com/MRT/go/msnnkwxp1020093182mrt/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Mon Oct 6 11:52:57 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Mon Oct 6 11:53:02 2008 Subject: [Histonet] out dated reagents In-Reply-To: Message-ID: <157A2692170F495280C8E18ABB00A542@wchsys.org> I have powdered stains that are probably 10 years old. I date dry chemicals when received and put a 10 year expiration date, if no expiration date is provided by the company. This has satisfied all my inspectors so far. I have discarded all the stains and chemicals we do not use anymore through a lab wide discard. Joyce Cline, Technical Specialist Hagerstown Medical Lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Monday, October 06, 2008 12:19 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] out dated reagents hello all, we were inspected the beginning of june by cap and one of our deficiency was anp.21382. it had to do with outdated reagents. my director want all of my dry chemicals gone!!!! I have gone through them and just have what I need for the special stains that we do in house. does anyone know do they put expiration dates on dry chems. we have things like light green, biebrich scarlet, periodic acid. I really hate to get rid of all of this but he thinks I should. what are others doing about this? I can see after you have made up things to not keep it long but dry I know many yrs ago the biological stain commission thought it would be meaningless and arbitrary to put expiration dates on stains. it would me more important to maintain a record of the use of the powdered stain. which is what we do when we do the stain with our controls. anyway just wondering if there were any thoughts out there about this. anita dudley providence hosp mobile alabama _________________________________________________________________ See how Windows Mobile brings your life together-at home, work, or on the go. http://clk.atdmt.com/MRT/go/msnnkwxp1020093182mrt/direct/01/________________ _______________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Oct 6 12:39:07 2008 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Oct 6 12:39:53 2008 Subject: [Histonet] (no subject) Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB0028AE86B@e2k3ms1.urmc-sh.rochester.edu> I am looking for some feedback on the Dako autostainer link (newest machine) and/or the Dako ACIS III slide imager. Our lab is negotiating a contract and I need some ammo to fight for or against it. Whatever the case may be. Thanks in advance. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 From liz <@t> premierlab.com Mon Oct 6 12:51:28 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Oct 6 12:51:40 2008 Subject: [Histonet] (no subject) In-Reply-To: <2CF6F6B05263EA4EBAB07781B51E5DB0028AE86B@e2k3ms1.urmc-sh.rochester.edu> References: <2CF6F6B05263EA4EBAB07781B51E5DB0028AE86B@e2k3ms1.urmc-sh.rochester.edu> Message-ID: We have been working with ours for about a month now and its nice. Takes a bit more with respects to programming the runs, but we are using it as an open system since we are in research and work primarly with animal tissue. But overall we really like it. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Monday, October 06, 2008 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) I am looking for some feedback on the Dako autostainer link (newest machine) and/or the Dako ACIS III slide imager. Our lab is negotiating a contract and I need some ammo to fight for or against it. Whatever the case may be. Thanks in advance. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Mon Oct 6 13:56:47 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Oct 6 13:56:55 2008 Subject: [Histonet] Sulfur staining, part 2 Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E64E2@nmdamailsvr.nmda.ad.nmsu.edu> Do I have any takers for my question about staining for sulfur deposition in the brain of a sheep? Anybody? Help? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From rjbuesa <@t> yahoo.com Mon Oct 6 14:54:37 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 6 14:54:44 2008 Subject: [Histonet] out dated reagents In-Reply-To: Message-ID: <414964.84715.qm@web65710.mail.ac4.yahoo.com> Anita: Dry chemicals, especially INORGANIC are natural products that exist in nature for millions of years and we just extract them. It is unwarranted to have an expiration date on them. Another thing is the condition of being anhydrous, and the periodic acid that you mention is a good example of a chemical that can absorb water and?your will need to correct for? its hydrated condition when weighing it to prepare a solution. Regarding powdered stains I have used until recently very good Merck aniline powders manufactured in Darmstad before the "Great War" and I am referring to WWI, that one ending in 1918 and they worked beautifully. Since this facts sometimes are sometimes?unknown to some talented CAP inspectors, I use to add a label to each of those bottles with dry inorganic chemicals, stating "No expiration date as per manufacturer", with my signature. It always worked fine. Ren? J. --- On Mon, 10/6/08, anita dudley wrote: From: anita dudley Subject: [Histonet] out dated reagents To: "Histonet@lists.utsouthwestern.edu" Date: Monday, October 6, 2008, 12:19 PM hello all, we were inspected the beginning of june by cap and one of our deficiency was anp.21382. it had to do with outdated reagents. my director want all of my dry chemicals gone!!!! I have gone through them and just have what I need for the special stains that we do in house. does anyone know do they put expiration dates on dry chems. we have things like light green, biebrich scarlet, periodic acid. I really hate to get rid of all of this but he thinks I should. what are others doing about this? I can see after you have made up things to not keep it long but dry I know many yrs ago the biological stain commission thought it would be meaningless and arbitrary to put expiration dates on stains. it would me more important to maintain a record of the use of the powdered stain. which is what we do when we do the stain with our controls. anyway just wondering if there were any thoughts out there about this. anita dudley providence hosp mobile alabama _________________________________________________________________ See how Windows Mobile brings your life together?at home, work, or on the go. http://clk.atdmt.com/MRT/go/msnnkwxp1020093182mrt/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Mon Oct 6 15:48:36 2008 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Oct 6 15:52:48 2008 Subject: [Histonet] out dated reagents In-Reply-To: <414964.84715.qm@web65710.mail.ac4.yahoo.com> References: , <414964.84715.qm@web65710.mail.ac4.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D29A21B99@LRGHEXVS1.practice.lrgh.org> Because I work in small lab, we currently buy everything pre made. Prior to that all I used to do was write my received day on by dry chemicals, open date and either write stable or no expiration date on the label. To date I have not had an issue with it for either CAP or CLIA. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com] Sent: Monday, October 06, 2008 3:54 PM To: Histonet@lists.utsouthwestern.edu; anita dudley Subject: Re: [Histonet] out dated reagents Anita: Dry chemicals, especially INORGANIC are natural products that exist in nature for millions of years and we just extract them. It is unwarranted to have an expiration date on them. Another thing is the condition of being anhydrous, and the periodic acid that you mention is a good example of a chemical that can absorb water and your will need to correct for its hydrated condition when weighing it to prepare a solution. Regarding powdered stains I have used until recently very good Merck aniline powders manufactured in Darmstad before the "Great War" and I am referring to WWI, that one ending in 1918 and they worked beautifully. Since this facts sometimes are sometimes unknown to some talented CAP inspectors, I use to add a label to each of those bottles with dry inorganic chemicals, stating "No expiration date as per manufacturer", with my signature. It always worked fine. Ren? J. --- On Mon, 10/6/08, anita dudley wrote: From: anita dudley Subject: [Histonet] out dated reagents To: "Histonet@lists.utsouthwestern.edu" Date: Monday, October 6, 2008, 12:19 PM hello all, we were inspected the beginning of june by cap and one of our deficiency was anp.21382. it had to do with outdated reagents. my director want all of my dry chemicals gone!!!! I have gone through them and just have what I need for the special stains that we do in house. does anyone know do they put expiration dates on dry chems. we have things like light green, biebrich scarlet, periodic acid. I really hate to get rid of all of this but he thinks I should. what are others doing about this? I can see after you have made up things to not keep it long but dry I know many yrs ago the biological stain commission thought it would be meaningless and arbitrary to put expiration dates on stains. it would me more important to maintain a record of the use of the powdered stain. which is what we do when we do the stain with our controls. anyway just wondering if there were any thoughts out there about this. anita dudley providence hosp mobile alabama _________________________________________________________________ See how Windows Mobile brings your life together?at home, work, or on the go. http://clk.atdmt.com/MRT/go/msnnkwxp1020093182mrt/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From rema.oliver <@t> unsw.edu.au Mon Oct 6 17:58:55 2008 From: rema.oliver <@t> unsw.edu.au (Rema) Date: Mon Oct 6 18:10:14 2008 Subject: [Histonet] Labeled particles Message-ID: <48EA982F.4020504@unsw.edu.au> - Hi, I need to detect particles that are covalently labelled either with fluorescein or carboxytetramethyrhodamine in rat liver using paraffin sections. Could someone please suggest the best way of doing this. Thank you, Rema -- Rema Oliver PhD Research Fellow Surgical & Orthopaedic Research Laboratories Level 1, Clinical Sciences Bldg Prince of Wales Hospital Ph: +61 2 9382 2654 Fax: +61 2 9382 2660 Mobile: 0419 605 034 From Susan.Walzer <@t> HCAHealthcare.com Tue Oct 7 06:43:48 2008 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Tue Oct 7 06:44:20 2008 Subject: [Histonet] Does anyone have any ideas on the use of softenerondecal blocks. Does this help 'soften' them? If so how? In-Reply-To: <74B6791EBFBF420A8E00F77D55FE7FFE@health.ad.jcu.edu.au> References: <48E4EA0E020000B500002A23@ekhgwia.ekht.nhs.uk><2EBB4E375CB948FA81930B5C49BC9F6F@pentium4> <74B6791EBFBF420A8E00F77D55FE7FFE@health.ad.jcu.edu.au> Message-ID: <471953BC63077941B82C26A4338272B42F061E@ORLEV03.hca.corpad.net> We face the block and put it in a dish with rapid decal solution. For tough blocks like uterus we soak them in a solution of water, ammonia(which is great for bloody tissue) and liquid soap. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Reilly Sent: Friday, October 03, 2008 7:58 PM To: 'Piero Nelva'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Does anyone have any ideas on the use of softenerondecal blocks. Does this help 'soften' them? If so how? Floating the trimmed/faced block on the waterbath for 2 minutes helps. Then put them on wet ice to cool. Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piero Nelva Sent: Friday, 3 October 2008 7:16 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Does anyone have any ideas on the use of softener ondecal blocks. Does this help 'soften' them? If so how? Hi Sarah I don't use a softener as such, but have had good results putting trimmed blocks on a water soaked tissue on the bench for 30 min or so just before cooling and sectioning. I find it is more effective than an ice bath at softening tissues. REgards Piero Nelva Anatomical Pathology Monash Medical Centre Australia ----- Original Message ----- From: "SARAH REEVES" To: Sent: Friday, October 03, 2008 12:34 AM Subject: [Histonet] Does anyone have any ideas on the use of softener on decal blocks. Does this help 'soften' them? If so how? Does anyone have any ideas on the use of softener on decal blocks. Does this help 'soften' them? If so how? Thanks in advance Sarah ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals University NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ---- ---- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.7.5/1704 - Release Date: 10/2/2008 9:35 PM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arsenn <@t> hsh.org Tue Oct 7 07:33:54 2008 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Tue Oct 7 07:34:01 2008 Subject: [Histonet] Waste on Benchmark XT Message-ID: ________________________________ From: Senn, Amy R Sent: Tuesday, October 07, 2008 8:33 AM To: Senn, Amy R Subject: Hello Histoland, We have a Benchmark XT. The sales rep told us it was ok to dump the waste down the drain. THEN we were told that the waste is hazardous and actually causes mutant changes in lab animals. (wow, I could turn into Rogue or Storm! My luck, I'd be Beast or Toad . . . . .) Anyway, can I get some feedback from you guys about how you dispose of the waste from the Benchmark XT? We had someone here to test it, but we're still waiting for results. Thanks! Amy, Camp Hill PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From LSebree <@t> uwhealth.org Tue Oct 7 07:45:07 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Oct 7 07:45:18 2008 Subject: [Histonet] Waste on Benchmark XT In-Reply-To: Message-ID: We figured out how much of the waste components were actually hazardous, i.e. DAB. Our University safety department then said that the small amount of DAB was safe to pour down the drain but they didn't want the LCS going down the drain. So we dump the aqueous part and the oil part we save and they (safety) come get it and incinerate it. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Tuesday, October 07, 2008 7:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste on Benchmark XT ________________________________ From: Senn, Amy R Sent: Tuesday, October 07, 2008 8:33 AM To: Senn, Amy R Subject: Hello Histoland, We have a Benchmark XT. The sales rep told us it was ok to dump the waste down the drain. THEN we were told that the waste is hazardous and actually causes mutant changes in lab animals. (wow, I could turn into Rogue or Storm! My luck, I'd be Beast or Toad . . . . .) Anyway, can I get some feedback from you guys about how you dispose of the waste from the Benchmark XT? We had someone here to test it, but we're still waiting for results. Thanks! Amy, Camp Hill PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Oct 7 07:46:52 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 7 07:46:57 2008 Subject: [Histonet] Waste on Benchmark XT In-Reply-To: Message-ID: <545123.14207.qm@web65708.mail.ac4.yahoo.com> Amy: The only thing I do not understand about your posting is the "testing part". What test are you writing about? Testing how hazardous the waste is? If a waste is hazardous, it is hazardous and cannot be be dumped down the drain. As a matter of fact no chemical or biological waste can be disposed that way. Some sales rep are willing to say anything in order to not jeopardize a sale. You will have to dispose your waste in the same way as you dispose of formalin, which I imagine you dispose through a waste management company, no? Ren? J. --- On Tue, 10/7/08, Senn, Amy R wrote: From: Senn, Amy R Subject: [Histonet] Waste on Benchmark XT To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 7, 2008, 8:33 AM ________________________________ From: Senn, Amy R Sent: Tuesday, October 07, 2008 8:33 AM To: Senn, Amy R Subject: Hello Histoland, We have a Benchmark XT. The sales rep told us it was ok to dump the waste down the drain. THEN we were told that the waste is hazardous and actually causes mutant changes in lab animals. (wow, I could turn into Rogue or Storm! My luck, I'd be Beast or Toad . . . . .) Anyway, can I get some feedback from you guys about how you dispose of the waste from the Benchmark XT? We had someone here to test it, but we're still waiting for results. Thanks! Amy, Camp Hill PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Oct 7 07:53:17 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Oct 7 07:53:21 2008 Subject: [Histonet] Waste on Benchmark XT In-Reply-To: Message-ID: <380984.70297.qm@web65705.mail.ac4.yahoo.com> Linda: With all due respect to your university safety department, the fact that they are basing their advise on the "dilution effect" of the sewer system over your waste, it will not just be your waste, but that of all other waste sources that may contain the same DAB and that they absolute do not know. Because one particular waste source is "small" it does not mean that "in aggregate"all those small sources can make a much larger polluting source. Dangerous wastes ought not to be discarded down the drain, no matter how small the "may seem to be". Ren? J. --- On Tue, 10/7/08, Sebree Linda A. wrote: From: Sebree Linda A. Subject: RE: [Histonet] Waste on Benchmark XT To: "Senn, Amy R" , histonet@lists.utsouthwestern.edu Date: Tuesday, October 7, 2008, 8:45 AM We figured out how much of the waste components were actually hazardous, i.e. DAB. Our University safety department then said that the small amount of DAB was safe to pour down the drain but they didn't want the LCS going down the drain. So we dump the aqueous part and the oil part we save and they (safety) come get it and incinerate it. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Tuesday, October 07, 2008 7:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste on Benchmark XT ________________________________ From: Senn, Amy R Sent: Tuesday, October 07, 2008 8:33 AM To: Senn, Amy R Subject: Hello Histoland, We have a Benchmark XT. The sales rep told us it was ok to dump the waste down the drain. THEN we were told that the waste is hazardous and actually causes mutant changes in lab animals. (wow, I could turn into Rogue or Storm! My luck, I'd be Beast or Toad . . . . .) Anyway, can I get some feedback from you guys about how you dispose of the waste from the Benchmark XT? We had someone here to test it, but we're still waiting for results. Thanks! Amy, Camp Hill PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Tue Oct 7 08:07:06 2008 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Oct 7 08:07:26 2008 Subject: [Histonet] Waste on Benchmark XT In-Reply-To: <380984.70297.qm@web65705.mail.ac4.yahoo.com> References: , <380984.70297.qm@web65705.mail.ac4.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D29A21B9A@LRGHEXVS1.practice.lrgh.org> First of all what does the MSDS state, and what does your state allow? New Hampshire's regulations are tighter than the EPA's and very little is allowed to be drain disposed. If the waste is flammable, corrosive, reactive or a EPA listed waste, it must be handled as a hazardous waste and disposed of accordingly. We have are waste hauled by a licensed waste hauler. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa [rjbuesa@yahoo.com] Sent: Tuesday, October 07, 2008 8:53 AM To: Senn, Amy R; histonet@lists.utsouthwestern.edu; Sebree Linda A. Subject: RE: [Histonet] Waste on Benchmark XT Linda: With all due respect to your university safety department, the fact that they are basing their advise on the "dilution effect" of the sewer system over your waste, it will not just be your waste, but that of all other waste sources that may contain the same DAB and that they absolute do not know. Because one particular waste source is "small" it does not mean that "in aggregate"all those small sources can make a much larger polluting source. Dangerous wastes ought not to be discarded down the drain, no matter how small the "may seem to be". Ren? J. --- On Tue, 10/7/08, Sebree Linda A. wrote: From: Sebree Linda A. Subject: RE: [Histonet] Waste on Benchmark XT To: "Senn, Amy R" , histonet@lists.utsouthwestern.edu Date: Tuesday, October 7, 2008, 8:45 AM We figured out how much of the waste components were actually hazardous, i.e. DAB. Our University safety department then said that the small amount of DAB was safe to pour down the drain but they didn't want the LCS going down the drain. So we dump the aqueous part and the oil part we save and they (safety) come get it and incinerate it. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Tuesday, October 07, 2008 7:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste on Benchmark XT ________________________________ From: Senn, Amy R Sent: Tuesday, October 07, 2008 8:33 AM To: Senn, Amy R Subject: Hello Histoland, We have a Benchmark XT. The sales rep told us it was ok to dump the waste down the drain. THEN we were told that the waste is hazardous and actually causes mutant changes in lab animals. (wow, I could turn into Rogue or Storm! My luck, I'd be Beast or Toad . . . . .) Anyway, can I get some feedback from you guys about how you dispose of the waste from the Benchmark XT? We had someone here to test it, but we're still waiting for results. Thanks! Amy, Camp Hill PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From laurie.colbert <@t> huntingtonhospital.com Tue Oct 7 09:19:56 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Oct 7 09:19:57 2008 Subject: [Histonet] Waste on Benchmark XT Message-ID: <57BE698966D5C54EAE8612E8941D768303E1C8AE@EXCHANGE3.huntingtonhospital.com> We have the same hazardous waste company that picks up our formalin, alcohol, xylene waste pick up all of the waste from the Benchmarks. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Tuesday, October 07, 2008 5:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste on Benchmark XT ________________________________ From: Senn, Amy R Sent: Tuesday, October 07, 2008 8:33 AM To: Senn, Amy R Subject: Hello Histoland, We have a Benchmark XT. The sales rep told us it was ok to dump the waste down the drain. THEN we were told that the waste is hazardous and actually causes mutant changes in lab animals. (wow, I could turn into Rogue or Storm! My luck, I'd be Beast or Toad . . . . .) Anyway, can I get some feedback from you guys about how you dispose of the waste from the Benchmark XT? We had someone here to test it, but we're still waiting for results. Thanks! Amy, Camp Hill PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Oct 7 10:05:47 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Oct 7 10:05:59 2008 Subject: [Histonet] Slide label printer Message-ID: <48EB428B0200007700005F81@gwmail6.harthosp.org> I know that this has been discussed before, but could someone recommend a software program and printer for making labels for glass microscopic slides that are sent out from our institution. Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From liz <@t> premierlab.com Tue Oct 7 10:20:22 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Oct 7 10:20:31 2008 Subject: [Histonet] Slide label printer In-Reply-To: <48EB428B0200007700005F81@gwmail6.harthosp.org> References: <48EB428B0200007700005F81@gwmail6.harthosp.org> Message-ID: Richard We buy the pin fed labels from stat lab and we have made a word document that we use to make our labels. I can send you a blank one, we just use the manuel feed on our laser printer. We just had to make a custom label format in word. It seems to work well for us, no cost for special printers etc. For labels for unstained sections we use the Dako labeler that comes with the autostainer and we use the software that's on the instrument. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, October 07, 2008 9:06 AM To: Histonet Subject: [Histonet] Slide label printer I know that this has been discussed before, but could someone recommend a software program and printer for making labels for glass microscopic slides that are sent out from our institution. Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MMCCOY <@t> lakelandregional.org Tue Oct 7 10:36:12 2008 From: MMCCOY <@t> lakelandregional.org (Mary McCoy) Date: Tue Oct 7 10:33:08 2008 Subject: [Histonet] Waste on Benchmark XT In-Reply-To: <57BE698966D5C54EAE8612E8941D768303E1C8AE@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D768303E1C8AE@EXCHANGE3.huntingtonhospital.com> Message-ID: <48EB49D9.D27C.00AB.0@lakelandregional.org> Each Waste Water Treatment System have their own standards. Most MSDS sheets say to check with your local Waste Water Treatment Plant for local regulations. They will approve or not whether any chemical can be put down the drain. Most do not allow Xylene as it is not miscible with water and is so flammable, but some might allow formalin or dilute amounts of DAB. Mary McCoy HTL(ASCP) Supervisor of Pathology Services Lakeland Regional Health System St. Joseph MI 49098 (269) 982-4891 mmccoy@lakelandregional.org >>> "Laurie Colbert" 10/7/2008 10:19 AM >>> We have the same hazardous waste company that picks up our formalin, alcohol, xylene waste pick up all of the waste from the Benchmarks. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Tuesday, October 07, 2008 5:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste on Benchmark XT ________________________________ From: Senn, Amy R Sent: Tuesday, October 07, 2008 8:33 AM To: Senn, Amy R Subject: Hello Histoland, We have a Benchmark XT. The sales rep told us it was ok to dump the waste down the drain. THEN we were told that the waste is hazardous and actually causes mutant changes in lab animals. (wow, I could turn into Rogue or Storm! My luck, I'd be Beast or Toad . . . . .) Anyway, can I get some feedback from you guys about how you dispose of the waste from the Benchmark XT? We had someone here to test it, but we're still waiting for results. Thanks! Amy, Camp Hill PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:McCoy, Mary TEL;WORK:982-4891 ORG:;Lab TEL;PREF;FAX:98-8258 EMAIL;WORK;PREF;NGW:MMcCOY@lakelandregional.org N:McCoy;Mary TITLE:Coordinator Pathology END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:McCoy, Mary TEL;WORK;PREF:982-4891 ORG:;Lab TEL;PREF;FAX:98-8258 EMAIL;WORK;PREF;NGW:MMcCOY@lakelandregional.org N:McCoy;Mary TITLE:Coordinator Pathology END:VCARD From JEllin <@t> yumaregional.org Tue Oct 7 10:45:01 2008 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Oct 7 10:45:09 2008 Subject: [Histonet] Waste on Benchmark XT References: <57BE698966D5C54EAE8612E8941D768303E1C8AE@EXCHANGE3.huntingtonhospital.com> Message-ID: <29BE166A2CF48D459853F8EC57CD37E801611205@EXCHANGECLUSTER.yumaregional.local> I am surprised that ,, and also Ventana has not informed you of a way to neutralize the waste.. I know that they have the protocols you might want to get intouch with them about this.. and also follow your facilites regulatory guidlines,, Jesus Ellin HT/PA ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Colbert Sent: Tue 10/7/2008 7:19 AM To: Senn, Amy R; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Waste on Benchmark XT We have the same hazardous waste company that picks up our formalin, alcohol, xylene waste pick up all of the waste from the Benchmarks. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://connect.yumaregional.org/CitrixFEI/composemessage.asp?to=histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Tuesday, October 07, 2008 5:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste on Benchmark XT ________________________________ From: Senn, Amy R Sent: Tuesday, October 07, 2008 8:33 AM To: Senn, Amy R Subject: Hello Histoland, We have a Benchmark XT. The sales rep told us it was ok to dump the waste down the drain. THEN we were told that the waste is hazardous and actually causes mutant changes in lab animals. (wow, I could turn into Rogue or Storm! My luck, I'd be Beast or Toad . . . . .) Anyway, can I get some feedback from you guys about how you dispose of the waste from the Benchmark XT? We had someone here to test it, but we're still waiting for results. Thanks! Amy, Camp Hill PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. From Maxim_71 <@t> mail.ru Tue Oct 7 14:07:25 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Tue Oct 7 14:08:37 2008 Subject: [Histonet] Does anyone have any ideas on the use of softenerondecal blocks. Does this help 'soften' them? If so how? Message-ID: <43347117.20081007230725@mail.ru> SARAH: If you will determine endpoint of decalcification with any method and then you will can be able to avoid similar problems. Maxim Peshkov Russia, Taganrog. You wrote at Friday, October 03, 2008 12:34 AM > Does anyone have any ideas on the use of softener on > decal blocks. Doesv this help 'soften' them? > If so how? > Thanks in advance > Sarah mailto:Maxim_71@mail.ru From marktarango <@t> gmail.com Tue Oct 7 15:11:19 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Oct 7 15:11:24 2008 Subject: [Histonet] MOHS cryostat cart Message-ID: <5b6eb13e0810071311x37c8d530nf72a1c771e7c7843@mail.gmail.com> Does anyone have a suggestion of a cart to carry around a cryostat for MOHS? I have a Leica CM 1100. Thanks Mark From shive003 <@t> umn.edu Tue Oct 7 15:14:35 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Oct 7 15:14:40 2008 Subject: [Histonet] platelets and IHC Message-ID: <0D01B368A9A74615AEDDDD9E2392802B@auxs.umn.edu> Hi all, Has anyone ever encountered platelets staining nonspecifically when using an antibody directed against other antigens? If so, how do you quench that activity? Thanks. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From shive003 <@t> umn.edu Tue Oct 7 17:27:15 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Oct 7 17:27:19 2008 Subject: [Histonet] Fw: platelets and IHC Message-ID: Addendum... I should have included this information in my original posting... I'm already doing a 3% H2O2 peroxidase quench (10-15 minutes) and a 10% normal serum protein block prior to primary Ab, and a 2-4% case species serum addition to the link Ab. Is there something ELSE that I could use to block binding to platelets (or what appears to be platelets, though it could be bits of proteinaceous cellular debris that's binding Ab nonspecifically)? Jan S ----- Original Message ----- From: Jan Shivers To: histonet Sent: Tuesday, October 07, 2008 3:14 PM Subject: platelets and IHC Hi all, Has anyone ever encountered platelets staining nonspecifically when using an antibody directed against other antigens? If so, how do you quench that activity? Thanks. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From jerry.santiago <@t> jax.ufl.edu Tue Oct 7 18:58:20 2008 From: jerry.santiago <@t> jax.ufl.edu (Santiago, Jerry) Date: Tue Oct 7 18:58:35 2008 Subject: [Histonet] Florida Society for Histotechnology Message-ID: <816DC61E1730E843A0BCF4CCFA1EFCF3065558@jaxmail.umc.ufl.edu> The Florida Society for Histotechnology is calling for abstracts. Meeting Dates: May 14-17, 2009 Place: Bahia Mar Beach Resort, Fort Lauderdale Please submit abstracts to fsh@fshgroup.org. Abstracts must be received by December 31, 2008. You may download an abstract form at www.fshgroup.org Selected abstracts will be notified by January 31, 2009. From maxdad <@t> wi.rr.com Tue Oct 7 22:45:29 2008 From: maxdad <@t> wi.rr.com (Brad Miller) Date: Tue Oct 7 22:45:25 2008 Subject: [Histonet] Waste on Benchmark XT References: Message-ID: <70BF6E5B52064395815916FAF795FF6B@BRAD> Amy, you should certainly contact the customer service folks at Ventana to see if they have heard of this before. I would suspect they have. If so, they may be able to give you some specific info about the waste that would help you. But to be certain, you can take the results from your analysis (yes Rene J, there are Analytical Laboratories all over the country that specialize in this type of test via organic extraction, toxicity bioassay, and other EPA regulated testing) and find out how they comply with your local code. You can find the local codes for your municipality on www.municode.com One thing you will want to be sure about, your municipality is (most likely) only concerned with the waste that comes out of the facility as a whole...or "end of pipe". That's helpful to you because your waste gets diluted many times over before it gets to the "end of pipe". And, yes, it is not legal to dilute waste like this for the sake of getting past the local waste enforcement, but if it gets diluted in the course of leaving the building, that's usually okay according to federal hazardous waste regulations (40 CFR 261) which can be found at http://ecfr.gpoaccess.gov Try not to worry too much about the alligators in the sewer..there is a bunch of nasty stuff much worse than LCS coming from that building if it's a hospital...I assure you. Just think about all the goo coming from sterile processing...yuck! Anyway I hope that helps. ----- Original Message ----- From: "Senn, Amy R" To: Sent: Tuesday, October 07, 2008 7:33 AM Subject: [Histonet] Waste on Benchmark XT ________________________________ From: Senn, Amy R Sent: Tuesday, October 07, 2008 8:33 AM To: Senn, Amy R Subject: Hello Histoland, We have a Benchmark XT. The sales rep told us it was ok to dump the waste down the drain. THEN we were told that the waste is hazardous and actually causes mutant changes in lab animals. (wow, I could turn into Rogue or Storm! My luck, I'd be Beast or Toad . . . . .) Anyway, can I get some feedback from you guys about how you dispose of the waste from the Benchmark XT? We had someone here to test it, but we're still waiting for results. Thanks! Amy, Camp Hill PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.7.6/1711 - Release Date: 10/6/2008 5:37 PM From tora.bardal <@t> bio.ntnu.no Wed Oct 8 04:21:20 2008 From: tora.bardal <@t> bio.ntnu.no (Tora Bardal) Date: Wed Oct 8 04:21:36 2008 Subject: [Histonet] books ISH Message-ID: <48EC7B90.6020106@bio.ntnu.no> Hi all Books, literature concerning in situ hybridization methods (whole mounts,sections) practical approaches. What's new, what's best? Tora From rjbuesa <@t> yahoo.com Wed Oct 8 07:03:18 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 8 07:03:29 2008 Subject: [Histonet] Waste on Benchmark XT In-Reply-To: <70BF6E5B52064395815916FAF795FF6B@BRAD> Message-ID: <19311.86784.qm@web65710.mail.ac4.yahoo.com> Brad: >From the practical point of view, what costs more, the analysis that may turn out to show the requirement of discarding by a specialized contractor any way, or just doing that?from the beginning? Are you going to test ALL your used reagents? For me there is a clear action to take: do not discard into the sewer and treat everything as hazardous and use the services of a specialized waste management company. The "principle" that we can add "bad stuff" because there is already a "lot of bad stuff" out there is, from the environmental point of view, just outrageous and totally irresponsible! Ren? J. --- On Tue, 10/7/08, Brad Miller wrote: From: Brad Miller Subject: Re: [Histonet] Waste on Benchmark XT To: "Senn, Amy R" , histonet@lists.utsouthwestern.edu Date: Tuesday, October 7, 2008, 11:45 PM Amy, you should certainly contact the customer service folks at Ventana to see if they have heard of this before. I would suspect they have. If so, they may be able to give you some specific info about the waste that would help you. But to be certain, you can take the results from your analysis (yes Rene J, there are Analytical Laboratories all over the country that specialize in this type of test via organic extraction, toxicity bioassay, and other EPA regulated testing) and find out how they comply with your local code. You can find the local codes for your municipality on www.municode.com One thing you will want to be sure about, your municipality is (most likely) only concerned with the waste that comes out of the facility as a whole...or "end of pipe". That's helpful to you because your waste gets diluted many times over before it gets to the "end of pipe". And, yes, it is not legal to dilute waste like this for the sake of getting past the local waste enforcement, but if it gets diluted in the course of leaving the building, that's usually okay according to federal hazardous waste regulations (40 CFR 261) which can be found at http://ecfr.gpoaccess.gov Try not to worry too much about the alligators in the sewer..there is a bunch of nasty stuff much worse than LCS coming from that building if it's a hospital...I assure you. Just think about all the goo coming from sterile processing...yuck! Anyway I hope that helps. ----- Original Message ----- From: "Senn, Amy R" To: Sent: Tuesday, October 07, 2008 7:33 AM Subject: [Histonet] Waste on Benchmark XT ________________________________ From: Senn, Amy R Sent: Tuesday, October 07, 2008 8:33 AM To: Senn, Amy R Subject: Hello Histoland, We have a Benchmark XT. The sales rep told us it was ok to dump the waste down the drain. THEN we were told that the waste is hazardous and actually causes mutant changes in lab animals. (wow, I could turn into Rogue or Storm! My luck, I'd be Beast or Toad . . . . .) Anyway, can I get some feedback from you guys about how you dispose of the waste from the Benchmark XT? We had someone here to test it, but we're still waiting for results. Thanks! Amy, Camp Hill PA From Wanda.Smith <@t> HCAhealthcare.com Wed Oct 8 08:01:59 2008 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Wed Oct 8 08:02:05 2008 Subject: [Histonet] Zinc Formalin for Bone Marrow Biopsies Message-ID: <817C2761C5A1394180709AEEDB775B7E06B1746F@NASEV03.hca.corpad.net> Good Morning, We have substituted Zinc Formalin for B5 for our BM bxs. I have a few questions I hope someone can help me with. 1. We increased fixation time 1.5 times from the B5. Question: Is there a maximum amount of time the bxs can stay in the ZF without causing harm to the bx? If a BM Bx stays in Zinc Formalin overnight, will that harm the cellular morphology? 2. Can Zinc Formalin be disposed of the same way we dispose of our 10% NBF? The B5 had to be picked up for special disposal due to the mercury content. Is there special handling for Zinc Formalin? Thanks and have a great day!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax From kynemitz <@t> travmax.com Wed Oct 8 08:25:15 2008 From: kynemitz <@t> travmax.com (Kyla Nemitz) Date: Wed Oct 8 08:32:27 2008 Subject: [Histonet] Permanent Histo position in Albuquerque, NM Message-ID: <9416D9FA37C1C04FA83D69684E95D2E71E4DE371@exbk2.maxhealth.com> Hello all - A facility in Albuquerque, NM is looking for a perm histo tech to work M-F day shift, 40 hours/week. They need someone ASCP for basic histology. They facility will also consider someone to work temp-to-perm. If you are interested in this position, please contact me. Thank you, hope to hear from you soon! Kyla Nemitz TravelMax Medical Professionals - Maxim Healthcare Tel: 888.800.1855 or 813.371.5175 Fax: 800.294.1248 Search our jobs or apply online at: www.TravelMaxAllied.com From SALAR <@t> sanfordhealth.org Wed Oct 8 08:33:51 2008 From: SALAR <@t> sanfordhealth.org (SALA,ROBERT) Date: Wed Oct 8 08:34:27 2008 Subject: [Histonet] MOHS cryostat cart In-Reply-To: <5b6eb13e0810071311x37c8d530nf72a1c771e7c7843@mail.gmail.com> Message-ID: Mark We have a Leica CM1100 that our paths use to do frozens at outlying hospitals and we use a cart from Ferro in Wilmington Ohio. It is a Universal 298VH Salesmaker Cart. This cart is actually pretty handy. It has a crank for raising and lowering, plus the top is wheeled and slides on and off the frame for easy loading in a vehicle. Bob Robert M. Sala M.T. (ASCP) Histology Supervisor Sanford Health P.O. Box 5039 1305 West 18th Street Sioux Falls, SD 57117-5039 salar@sanfordhealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Tuesday, October 07, 2008 3:11 PM To: histonet Subject: [Histonet] MOHS cryostat cart Does anyone have a suggestion of a cart to carry around a cryostat for MOHS? I have a Leica CM 1100. Thanks Mark _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain privileged and confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From HornHV <@t> archildrens.org Wed Oct 8 08:41:26 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Oct 8 08:41:37 2008 Subject: [Histonet] Zinc Formalin for Bone Marrow Biopsies In-Reply-To: <817C2761C5A1394180709AEEDB775B7E06B1746F@NASEV03.hca.corpad.net> References: <817C2761C5A1394180709AEEDB775B7E06B1746F@NASEV03.hca.corpad.net> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82E5B@EMAIL.archildrens.org> We use zinc formalin to fix all of our tissues. It will not harm the biopsy to left overnight. Our formalin is disposed of as hazardous waste and would be even without the zinc. You will need to wash the bone marrows after fixation as zinc formalin does not mix well with regular buffered formalin. A cloudy precipitate will form if you do not wash after zinc fixation. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Wednesday, October 08, 2008 8:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Zinc Formalin for Bone Marrow Biopsies Good Morning, We have substituted Zinc Formalin for B5 for our BM bxs. I have a few questions I hope someone can help me with. 1. We increased fixation time 1.5 times from the B5. Question: Is there a maximum amount of time the bxs can stay in the ZF without causing harm to the bx? If a BM Bx stays in Zinc Formalin overnight, will that harm the cellular morphology? 2. Can Zinc Formalin be disposed of the same way we dispose of our 10% NBF? The B5 had to be picked up for special disposal due to the mercury content. Is there special handling for Zinc Formalin? Thanks and have a great day!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From paintedsplashes <@t> yahoo.com Wed Oct 8 08:58:04 2008 From: paintedsplashes <@t> yahoo.com (Jeanne Clark) Date: Wed Oct 8 08:58:11 2008 Subject: [Histonet] from CAP ANP.22993 Message-ID: <960278.85178.qm@web30708.mail.mud.yahoo.com> From paintedsplashes <@t> yahoo.com Wed Oct 8 09:00:22 2008 From: paintedsplashes <@t> yahoo.com (Jeanne Clark) Date: Wed Oct 8 09:00:31 2008 Subject: [Histonet] CAP ? Message-ID: <940251.32021.qm@web30702.mail.mud.yahoo.com> Sorry, message did not go thru. ? ANP.22993 corrective action taken for unacceptable results? ? ER/PR interpret not what CAP expected.? Other labs - also using LabVision instrumentation - had similar results to us.? How do you address this? ? Jeanne From rich.strauss <@t> Esoterix.com Wed Oct 8 09:05:40 2008 From: rich.strauss <@t> Esoterix.com (Strauss, Rich) Date: Wed Oct 8 09:05:53 2008 Subject: [Histonet] Waste on Benchmark XT In-Reply-To: <19311.86784.qm@web65710.mail.ac4.yahoo.com> References: <70BF6E5B52064395815916FAF795FF6B@BRAD> <19311.86784.qm@web65710.mail.ac4.yahoo.com> Message-ID: We recently went through this the testing and the status saved us money as that it was not considered hazardous but because of trace components like the sodium azide we are having it carted away. It is under a reduced risk category and not being charged the same as DAB off of other instruments. We had the potential waste removal company test it for us at a reasonable price. Richard Strauss HT, QIHC IHC/Histology Supervisor USL-East 201 Summit View Dr Brentwood, TN 37027 phone: 615 377-7151 rich.strauss@esoterix.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, October 08, 2008 7:03 AM To: Senn, Amy R; histonet@lists.utsouthwestern.edu; Brad Miller Subject: Re: [Histonet] Waste on Benchmark XT Brad: >From the practical point of view, what costs more, the analysis that may turn out to show the requirement of discarding by a specialized contractor any way, or just doing that?from the beginning? Are you going to test ALL your used reagents? For me there is a clear action to take: do not discard into the sewer and treat everything as hazardous and use the services of a specialized waste management company. The "principle" that we can add "bad stuff" because there is already a "lot of bad stuff" out there is, from the environmental point of view, just outrageous and totally irresponsible! Ren? J. --- On Tue, 10/7/08, Brad Miller wrote: From: Brad Miller Subject: Re: [Histonet] Waste on Benchmark XT To: "Senn, Amy R" , histonet@lists.utsouthwestern.edu Date: Tuesday, October 7, 2008, 11:45 PM Amy, you should certainly contact the customer service folks at Ventana to see if they have heard of this before. I would suspect they have. If so, they may be able to give you some specific info about the waste that would help you. But to be certain, you can take the results from your analysis (yes Rene J, there are Analytical Laboratories all over the country that specialize in this type of test via organic extraction, toxicity bioassay, and other EPA regulated testing) and find out how they comply with your local code. You can find the local codes for your municipality on www.municode.com One thing you will want to be sure about, your municipality is (most likely) only concerned with the waste that comes out of the facility as a whole...or "end of pipe". That's helpful to you because your waste gets diluted many times over before it gets to the "end of pipe". And, yes, it is not legal to dilute waste like this for the sake of getting past the local waste enforcement, but if it gets diluted in the course of leaving the building, that's usually okay according to federal hazardous waste regulations (40 CFR 261) which can be found at http://ecfr.gpoaccess.gov Try not to worry too much about the alligators in the sewer..there is a bunch of nasty stuff much worse than LCS coming from that building if it's a hospital...I assure you. Just think about all the goo coming from sterile processing...yuck! Anyway I hope that helps. ----- Original Message ----- From: "Senn, Amy R" To: Sent: Tuesday, October 07, 2008 7:33 AM Subject: [Histonet] Waste on Benchmark XT ________________________________ From: Senn, Amy R Sent: Tuesday, October 07, 2008 8:33 AM To: Senn, Amy R Subject: Hello Histoland, We have a Benchmark XT. The sales rep told us it was ok to dump the waste down the drain. THEN we were told that the waste is hazardous and actually causes mutant changes in lab animals. (wow, I could turn into Rogue or Storm! My luck, I'd be Beast or Toad . . . . .) Anyway, can I get some feedback from you guys about how you dispose of the waste from the Benchmark XT? We had someone here to test it, but we're still waiting for results. Thanks! Amy, Camp Hill PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Oct 8 09:19:48 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Oct 8 09:19:58 2008 Subject: [Histonet] conjugated anti-FITC antibody Message-ID: <103844.33910.qm@web50308.mail.re2.yahoo.com> Hello All! I was wondering if anyone was aware of an anti-FITC antibody that was conjugated to either HRP or Biotin (eg?HRP-rabbit anti-FITC or Biotinylated rabbit anti-FITC).? If such a thing exists, can you tell me who sells it? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From shive003 <@t> umn.edu Wed Oct 8 09:28:24 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Oct 8 09:28:30 2008 Subject: [Histonet] Neospora antibody source Message-ID: Does anyone know of a good source for a Neospora caninum antibody, other than VMRD? Thanks in advance. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From jstaruk <@t> masshistology.com Wed Oct 8 11:07:32 2008 From: jstaruk <@t> masshistology.com (jstaruk) Date: Wed Oct 8 11:07:33 2008 Subject: [Histonet] Simple (?) equation question In-Reply-To: <898D946569A27444B65667A49C07405201DFE459@mailbe06.mc.vanderbilt.edu> Message-ID: Hello, I want to make a 2% HCl and 8% formic acid decalcifying solution. Since straight HCl is 37%, do I treat this as 100% (like you do using formalin), so the formula will simply be 98 water and 2 HCl or do I have to fraction it out so a 2% solution will actually be 95 water to 5 HCl? Also, the same question is for the formic acid, which is actually 88% My choices to make a 2% HCl and 8% formic acid solutions is either: A) 2 HCl, 8 formic acid and 90 water Or B) 5 HCl, 8.8 formic acid and 86.2 water Thanks Jim From jennifer.harvey <@t> Vanderbilt.Edu Wed Oct 8 11:16:51 2008 From: jennifer.harvey <@t> Vanderbilt.Edu (Harvey, Jennifer Lynn) Date: Wed Oct 8 11:17:00 2008 Subject: [Histonet] microscope slide glass quality Message-ID: Hi Histonet, I have been having this issue for a while now when companies try to get me to switch to their slides. If they are not 100% white glass, can it be an issue with fluorescent applications? I would love to save over 100 dollars a case but I do fluorescence often and don't want an added problem. Does anyone know if the slides with the green glass work for fluoresces? Thanks Jennifer Harvey, HT(ASCP) QIHC Vanderbilt Vision Reasearch Center Core Histologist RM 8105 MCE North Tower 1215 21st Ave. South Nashville, TN 37232-8808 Phone 615-936-1486 From rjbuesa <@t> yahoo.com Wed Oct 8 11:20:30 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 8 11:20:36 2008 Subject: [Histonet] Simple (?) equation question In-Reply-To: Message-ID: <251435.65088.qm@web65713.mail.ac4.yahoo.com> Depends on what do you want to do. IF you want to prepare a 1 Normal (= 1 Molar) solution, then you will have to consider the dilution factor. IF you want to prepare just a 2% volume/volume solution, then you can do it as you describe. Ren? J. --- On Wed, 10/8/08, jstaruk wrote: From: jstaruk Subject: [Histonet] Simple (?) equation question To: histonet@lists.utsouthwestern.edu Date: Wednesday, October 8, 2008, 12:07 PM Hello, I want to make a 2% HCl and 8% formic acid decalcifying solution. Since straight HCl is 37%, do I treat this as 100% (like you do using formalin), so the formula will simply be 98 water and 2 HCl or do I have to fraction it out so a 2% solution will actually be 95 water to 5 HCl? Also, the same question is for the formic acid, which is actually 88% My choices to make a 2% HCl and 8% formic acid solutions is either: A) 2 HCl, 8 formic acid and 90 water Or B) 5 HCl, 8.8 formic acid and 86.2 water Thanks Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Oct 8 11:25:12 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 8 11:25:21 2008 Subject: [Histonet] microscope slide glass quality In-Reply-To: Message-ID: <723826.62466.qm@web65702.mail.ac4.yahoo.com> Domestic slides are almost entirely (about 98% of the market) are manufacturer by Erie Glass Co., so the differences will be between the different glass quality but it is almost sure that all come from the same manufacturer. Try to contact them directly. Ren? J. --- On Wed, 10/8/08, Harvey, Jennifer Lynn wrote: From: Harvey, Jennifer Lynn Subject: [Histonet] microscope slide glass quality To: Histonet@lists.utsouthwestern.edu Date: Wednesday, October 8, 2008, 12:16 PM Hi Histonet, I have been having this issue for a while now when companies try to get me to switch to their slides. If they are not 100% white glass, can it be an issue with fluorescent applications? I would love to save over 100 dollars a case but I do fluorescence often and don't want an added problem. Does anyone know if the slides with the green glass work for fluoresces? Thanks Jennifer Harvey, HT(ASCP) QIHC Vanderbilt Vision Reasearch Center Core Histologist RM 8105 MCE North Tower 1215 21st Ave. South Nashville, TN 37232-8808 Phone 615-936-1486 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From maxdad <@t> wi.rr.com Wed Oct 8 11:29:05 2008 From: maxdad <@t> wi.rr.com (Brad Miller) Date: Wed Oct 8 11:29:03 2008 Subject: [Histonet] Waste on Benchmark XT References: <19311.86784.qm@web65710.mail.ac4.yahoo.com> Message-ID: <9839A632164B43F7AACCF40B5075980D@BRAD> Hi Rene. To answer your first question, I would have to echo what Rich Strauss said in his post. If the waste is found to be acceptable according to the EPA and local code, then the cost of using a special contractor is unnecessary and is therefore creating additional operational expense that is not needed. The scientists at the EPA and your local municipal waste treatment facilities are pretty smart and can even be downright protective about their systems. For that reason, my humble opinion would be that to second guess their directives in the name of environmentalism would not be necessary. If you have the money to hire a contractor to dispose of all of your used reagents, then I think that's great...but there may be a better use for that money. I would have to warn, and I'm sure you're already aware, that the specialized contractor does not have a magic wand that is waved over the waste. From a purely environmental standpoint, this can be somewhat wasteful in itself, if unnecessary. I can think of 4 reasons, just off the top of my head: 1.. These contractors tend to use very large furnaces and tremendous amounts of energy to burn much of the waste. If everyone sent unnecessary effluent to the specialized contractor (as many people do) then think of the amounts of energy (electricity, fuel, whatever) used for no reason. I wouldn't call this behavior outrageous or irresponsible, though...just uninformed. 2.. Also, while precautions are taken to reduce air pollution through this type of disposal, it still happens (see below). 3.. This is to say nothing about the occasional leaky container that winds up draining into the storm drain which does not have the same capacity to deal with waste that the sewer system does. 4.. And finally, the untold part of this type of disposal is that these contractors can be located in any area zoned as "commercial" which means they can even be dangerously located near neighborhoods. Does anyone remember the town of Apex in North Carolina that needed to be evacuated in 2006 because of a "chemical fire"? That was one of these hazardous waste facilities. You can read about it here http://www.csb.gov/index.cfm?folder=news_releases&page=news&NEWS_ID=311 To be environmentally conscious is absolutely the way to go, but to educate one's self about the cause can yield even better results! And I would agree, Rene, that any principle that claims that bad stuff is okay because there's other bad stuff out there would not be valid or responsible. But if that's a reference to my comment about sterile processing, then I would have to say respectfully that you may have missed my point. That point being that if the waste coming from the XT is deemed acceptable to the EPA and local code, then Amy and her facility most certainly have much larger and more valid concerns than the acceptable waste from the instrument. Does that make sense? ----- Original Message ----- From: Rene J Buesa To: Senn, Amy R ; histonet@lists.utsouthwestern.edu ; Brad Miller Sent: Wednesday, October 08, 2008 7:03 AM Subject: Re: [Histonet] Waste on Benchmark XT Brad: From the practical point of view, what costs more, the analysis that may turn out to show the requirement of discarding by a specialized contractor any way, or just doing that from the beginning? Are you going to test ALL your used reagents? For me there is a clear action to take: do not discard into the sewer and treat everything as hazardous and use the services of a specialized waste management company. The "principle" that we can add "bad stuff" because there is already a "lot of bad stuff" out there is, from the environmental point of view, just outrageous and totally irresponsible! Ren? J. --- On Tue, 10/7/08, Brad Miller wrote: From: Brad Miller Subject: Re: [Histonet] Waste on Benchmark XT To: "Senn, Amy R" , histonet@lists.utsouthwestern.edu Date: Tuesday, October 7, 2008, 11:45 PM Amy, you should certainly contact the customer service folks at Ventana to see if they have heard of this before. I would suspect they have. If so, they may be able to give you some specific info about the waste that would help you. But to be certain, you can take the results from your analysis (yes Rene J, there are Analytical Laboratories all over the country that specialize in this type of test via organic extraction, toxicity bioassay, and other EPA regulated testing) and find out how they comply with your local code. You can find the local codes for your municipality on www.municode.com One thing you will want to be sure about, your municipality is (most likely) only concerned with the waste that comes out of the facility as a whole...or "end of pipe". That's helpful to you because your waste gets diluted many times over before it gets to the "end of pipe". And, yes, it is not legal to dilute waste like this for the sake of getting past the local waste enforcement, but if it gets diluted in the course of leaving the building, that's usually okay according to federal hazardous waste regulations (40 CFR 261) which can be found at http://ecfr.gpoaccess.gov Try not to worry too much about the alligators in the sewer..there is a bunch of nasty stuff much worse than LCS coming from that building if it's a hospital...I assure you. Just think about all the goo coming from sterile processing...yuck! Anyway I hope that helps. ----- Original Message ----- From: "Senn, Amy R" To: Sent: Tuesday, October 07, 2008 7:33 AM Subject: [Histonet] Waste on Benchmark XT ________________________________ From: Senn, Amy R Sent: Tuesday, October 07, 2008 8:33 AM To: Senn, Amy R Subject: Hello Histoland, We have a Benchmark XT. The sales rep told us it was ok to dump the waste down the drain. THEN we were told that the waste is hazardous and actually causes mutant changes in lab animals. (wow, I could turn into Rogue or Storm! My luck, I'd be Beast or Toad . . . . .) Anyway, can I get some feedback from you guys about how you dispose of the waste from the Benchmark XT? We had someone here to test it, but we're still waiting for results. Thanks! Amy, Camp Hill PA ------------------------------------------------------------------------------ No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.7.6/1714 - Release Date: 10/8/2008 7:01 AM From jdooley2008 <@t> yahoo.com Wed Oct 8 11:31:40 2008 From: jdooley2008 <@t> yahoo.com (James Dooley) Date: Wed Oct 8 11:31:47 2008 Subject: [Histonet] paraffin sections Message-ID: <764063.10074.qm@web45911.mail.sp1.yahoo.com> I am a beginning to do paraffin section. I need to know the following as I have heard many things and I have received my best advice here. As of this moment I was storing them at 4 degrees following sections. The problem I am having is many of my section have been coming off when I deparaffinize the tissue. I have been baking the tissue for 60 minutes prior to the deparaffiniztion step. 1. How to store them? 2. How long they can be stored? 3. Under what conditions should they be stored? 4. When melting the paraffin how long should this be done for and at what temperture prior to deparaffinization? Thank you, James From LSebree <@t> uwhealth.org Wed Oct 8 11:33:35 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Wed Oct 8 11:33:45 2008 Subject: [Histonet] Waste on Benchmark XT In-Reply-To: <9839A632164B43F7AACCF40B5075980D@BRAD> Message-ID: Well stated Brad. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brad Miller Sent: Wednesday, October 08, 2008 11:29 AM To: rjbuesa@yahoo.com; Senn, Amy R; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Waste on Benchmark XT Hi Rene. To answer your first question, I would have to echo what Rich Strauss said in his post. If the waste is found to be acceptable according to the EPA and local code, then the cost of using a special contractor is unnecessary and is therefore creating additional operational expense that is not needed. The scientists at the EPA and your local municipal waste treatment facilities are pretty smart and can even be downright protective about their systems. For that reason, my humble opinion would be that to second guess their directives in the name of environmentalism would not be necessary. If you have the money to hire a contractor to dispose of all of your used reagents, then I think that's great...but there may be a better use for that money. I would have to warn, and I'm sure you're already aware, that the specialized contractor does not have a magic wand that is waved over the waste. From a purely environmental standpoint, this can be somewhat wasteful in itself, if unnecessary. I can think of 4 reasons, just off the top of my head: 1.. These contractors tend to use very large furnaces and tremendous amounts of energy to burn much of the waste. If everyone sent unnecessary effluent to the specialized contractor (as many people do) then think of the amounts of energy (electricity, fuel, whatever) used for no reason. I wouldn't call this behavior outrageous or irresponsible, though...just uninformed. 2.. Also, while precautions are taken to reduce air pollution through this type of disposal, it still happens (see below). 3.. This is to say nothing about the occasional leaky container that winds up draining into the storm drain which does not have the same capacity to deal with waste that the sewer system does. 4.. And finally, the untold part of this type of disposal is that these contractors can be located in any area zoned as "commercial" which means they can even be dangerously located near neighborhoods. Does anyone remember the town of Apex in North Carolina that needed to be evacuated in 2006 because of a "chemical fire"? That was one of these hazardous waste facilities. You can read about it here http://www.csb.gov/index.cfm?folder=news_releases&page=news&NEWS_ID=311 To be environmentally conscious is absolutely the way to go, but to educate one's self about the cause can yield even better results! And I would agree, Rene, that any principle that claims that bad stuff is okay because there's other bad stuff out there would not be valid or responsible. But if that's a reference to my comment about sterile processing, then I would have to say respectfully that you may have missed my point. That point being that if the waste coming from the XT is deemed acceptable to the EPA and local code, then Amy and her facility most certainly have much larger and more valid concerns than the acceptable waste from the instrument. Does that make sense? ----- Original Message ----- From: Rene J Buesa To: Senn, Amy R ; histonet@lists.utsouthwestern.edu ; Brad Miller Sent: Wednesday, October 08, 2008 7:03 AM Subject: Re: [Histonet] Waste on Benchmark XT Brad: From the practical point of view, what costs more, the analysis that may turn out to show the requirement of discarding by a specialized contractor any way, or just doing that from the beginning? Are you going to test ALL your used reagents? For me there is a clear action to take: do not discard into the sewer and treat everything as hazardous and use the services of a specialized waste management company. The "principle" that we can add "bad stuff" because there is already a "lot of bad stuff" out there is, from the environmental point of view, just outrageous and totally irresponsible! Ren? J. --- On Tue, 10/7/08, Brad Miller wrote: From: Brad Miller Subject: Re: [Histonet] Waste on Benchmark XT To: "Senn, Amy R" , histonet@lists.utsouthwestern.edu Date: Tuesday, October 7, 2008, 11:45 PM Amy, you should certainly contact the customer service folks at Ventana to see if they have heard of this before. I would suspect they have. If so, they may be able to give you some specific info about the waste that would help you. But to be certain, you can take the results from your analysis (yes Rene J, there are Analytical Laboratories all over the country that specialize in this type of test via organic extraction, toxicity bioassay, and other EPA regulated testing) and find out how they comply with your local code. You can find the local codes for your municipality on www.municode.com One thing you will want to be sure about, your municipality is (most likely) only concerned with the waste that comes out of the facility as a whole...or "end of pipe". That's helpful to you because your waste gets diluted many times over before it gets to the "end of pipe". And, yes, it is not legal to dilute waste like this for the sake of getting past the local waste enforcement, but if it gets diluted in the course of leaving the building, that's usually okay according to federal hazardous waste regulations (40 CFR 261) which can be found at http://ecfr.gpoaccess.gov Try not to worry too much about the alligators in the sewer..there is a bunch of nasty stuff much worse than LCS coming from that building if it's a hospital...I assure you. Just think about all the goo coming from sterile processing...yuck! Anyway I hope that helps. ----- Original Message ----- From: "Senn, Amy R" To: Sent: Tuesday, October 07, 2008 7:33 AM Subject: [Histonet] Waste on Benchmark XT ________________________________ From: Senn, Amy R Sent: Tuesday, October 07, 2008 8:33 AM To: Senn, Amy R Subject: Hello Histoland, We have a Benchmark XT. The sales rep told us it was ok to dump the waste down the drain. THEN we were told that the waste is hazardous and actually causes mutant changes in lab animals. (wow, I could turn into Rogue or Storm! My luck, I'd be Beast or Toad . . . . .) Anyway, can I get some feedback from you guys about how you dispose of the waste from the Benchmark XT? We had someone here to test it, but we're still waiting for results. Thanks! Amy, Camp Hill PA ------------------------------------------------------------------------------ No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.7.6/1714 - Release Date: 10/8/2008 7:01 AM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Oct 8 11:34:15 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Oct 8 11:34:26 2008 Subject: [Histonet] Simple (?) equation question In-Reply-To: References: <898D946569A27444B65667A49C07405201DFE459@mailbe06.mc.vanderbilt.edu> Message-ID: This is the way I would do it. Someone can correct me if I'm wrong. I consider the HCL to be 100% and the Formic acid to be just 88% at least that's the way we make up our formic acid for decal here (our stock formic acid is at 98%). You just need to use the simple equation V1xC1 = V2xC2 and perform two equations. Volume 1 - volume of stock reagent - this is what you want to figure out Volume 2 - volume of diluted reagent 100mls Concentration 1 - concentration of stock reagent - 88% formic acid ---- 100% HCL Concentration 2 - concentration of diluted reagent - 8% formic acid ----- 2% HCL FOR 2% HCL X x 100% = 100mls x 2% X = 2 mls of HCL acid FOR 8% Formic Acid X x 88% = 100mls x 8% X = 9.01 mls of Formic acid So you would add 2 mls of HCL and 9.01 mls of Formic Acid and 88.99 mls of dH20 (just subtracted from 100mls the final volume with the 2 amounts of acid to get 88.99) Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, October 08, 2008 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Simple (?) equation question Hello, I want to make a 2% HCl and 8% formic acid decalcifying solution. Since straight HCl is 37%, do I treat this as 100% (like you do using formalin), so the formula will simply be 98 water and 2 HCl or do I have to fraction it out so a 2% solution will actually be 95 water to 5 HCl? Also, the same question is for the formic acid, which is actually 88% My choices to make a 2% HCl and 8% formic acid solutions is either: A) 2 HCl, 8 formic acid and 90 water Or B) 5 HCl, 8.8 formic acid and 86.2 water Thanks Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Wed Oct 8 12:15:45 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Wed Oct 8 12:15:52 2008 Subject: [Histonet] Fw: platelets and IHC In-Reply-To: References: Message-ID: Can you post a pic of this non specific staining? What organ is it? Is it inside blood vessels? At 5:27 PM -0500 10/7/08, Jan Shivers wrote: >Addendum... I should have included this information in my original posting... > >I'm already doing a 3% H2O2 peroxidase quench (10-15 minutes) and a >10% normal serum protein block prior to primary Ab, and a 2-4% case >species serum addition to the link Ab. > >Is there something ELSE that I could use to block binding to >platelets (or what appears to be platelets, though it could be bits >of proteinaceous cellular debris that's binding Ab nonspecifically)? > >Jan S > >----- Original Message ----- >From: Jan Shivers >To: histonet >Sent: Tuesday, October 07, 2008 3:14 PM >Subject: platelets and IHC > > >Hi all, > >Has anyone ever encountered platelets staining nonspecifically when >using an antibody directed against other antigens? If so, how do >you quench that activity? Thanks. > >Jan Shivers >Senior Scientist >Histology/IHC/EM Section Head >University of Minnesota >Veterinary Diagnostic Laboratory >1333 Gortner Ave. >St. Paul, MN 55108 >612-624-7297 >shive003@umn.edu >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From leiker <@t> buffalo.edu Wed Oct 8 13:32:32 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Wed Oct 8 13:32:38 2008 Subject: [Histonet] paraffin sections In-Reply-To: <764063.10074.qm@web45911.mail.sp1.yahoo.com> References: <764063.10074.qm@web45911.mail.sp1.yahoo.com> Message-ID: Hi James, While I know that others with more experience are going to reply and have very good insights to add, in my few years of experience I've stored the paraffin sections at room temp. for up to several months. Sections are stored only after baking them in a 60 C for 1 hour. This baking is done after the cut slides have air dried overnight. I have not had problems with the sections falling off. I know that optimum paraffin section storage is a topic of debate - ambient air vs. nitrogen vs. covered in paraffin; 4 C vs. room temp., as well as the length of storage. While I haven't seen any report of sections falling off due to how they are stored, maybe others on this list have. There may be other issues, like what type of slides or paraffin blend you use. In our lab we use Superfrost Adhesive Slides Platinum Line (Mercedes Medical) and Polyfin (paraffin blended with plasticizers). Also, epitope and tissue type may be a factor. I stain for VWF, Beta-catenin, and cytoskeletal markers such as Myosin Heavy Chain and Tropopin I, all on rodent skeletal muscle and heart tissues, and so far haven't had any problems that I can tell are due to how the sections are stored. Hope this helps in some way! Merced --On Wednesday, October 08, 2008 9:31 AM -0700 James Dooley wrote: > I am a beginning to do paraffin section. I need to know the following as > I have heard many things and I have received my best advice here. As of > this moment I was storing them at 4 degrees following sections. The > problem I am having is many of my section have been coming off when I > deparaffinize the tissue. I have been baking the tissue for 60 minutes > prior to the deparaffiniztion step. > > 1. How to store them? > 2. How long they can be stored? > 3. Under what conditions should they be stored? > 4. When melting the paraffin how long should this be done for and at what > temperature prior to deparaffinization? > > > Thank you, > James > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 In order to put yourself in someone else's shoes, you must first take off yours. From enrriq88 <@t> yahoo.com Wed Oct 8 15:39:39 2008 From: enrriq88 <@t> yahoo.com (Jaime Plata) Date: Wed Oct 8 15:39:43 2008 Subject: [Histonet] Cytology Cell block Preparation Message-ID: <453346.39157.qm@web50403.mail.re2.yahoo.com> Hello fellows. I am Cyto/ Histology Sup. If there is any one with Non-GN or GN procedure on cell block preparation or methodology please share with me your ideas or procedure. I need also feedback on Thrombin Clot method. Thanks Jaime Plata MT HTL (ASCP) enrriq88@yahoo.com From pattennj <@t> mail.nih.gov Wed Oct 8 16:22:51 2008 From: pattennj <@t> mail.nih.gov (Patten, Nicole (NIH/NIAAA) [F]) Date: Wed Oct 8 16:22:57 2008 Subject: [Histonet] citrisolve? Message-ID: Does anyone have any experience using Citrisolve as a Xylene alternative to deparaffinize tissue sections? Do I use it just like Xylene for the same amount of time? Can it be reused or should I use new Citrisolve each time? How do I dispose of it? Any help at all would be much appreciated. Thanks! Nicole J. Patten Post-Baccalaureate Fellow/IRTA National Institutes of Health From kbowden <@t> ucsd.edu Wed Oct 8 16:37:36 2008 From: kbowden <@t> ucsd.edu (kbowden) Date: Wed Oct 8 16:37:43 2008 Subject: [Histonet] citrisolve? In-Reply-To: References: Message-ID: <48ED2820.3060801@ucsd.edu> I have used Citrisolve for serveral years. I don't think anything compares to Xylene, but I do think this comes very close. I change it about once a week. It pretty much deparaffinizes in the same amount of time. Disposal is dependent on the regulations in your area. When I started using it our Environmental Health and Safety said pour it down the drain, but as time goes by and with more regulations now I have to collect it and have it removed with hazardous waste. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. Patten, Nicole (NIH/NIAAA) [F] wrote: > Does anyone have any experience using Citrisolve as a Xylene alternative > to deparaffinize tissue sections? Do I use it just like Xylene for the > same amount of time? Can it be reused or should I use new Citrisolve > each time? How do I dispose of it? > > > > Any help at all would be much appreciated. Thanks! > > > > Nicole J. Patten > Post-Baccalaureate Fellow/IRTA > National Institutes of Health > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jonesly <@t> mir.wustl.edu Wed Oct 8 17:18:40 2008 From: jonesly <@t> mir.wustl.edu (Jones, Lynne) Date: Wed Oct 8 17:17:19 2008 Subject: [Histonet] RE: Histonet Digest, Decalcifying sol'n recipe In-Reply-To: <182e97f9-2a52-4f20-a06d-60a938b297bc> References: <182e97f9-2a52-4f20-a06d-60a938b297bc> Message-ID: <457977CBDB4DD64BA37509AC073943D9EA5DEDA329@RAD-VMSRVEXV2.rad.wustl.edu> The concentrated 37% HCl (more or less 12N) is weight by weight, not weight by volume - I'm too tired to figure out how that affects the math, so I Googled for a recipe. The folks at CSH are pretty reliable, and having a reference is always nice if people question the math. Recommended concentrations for formic acid are pretty variable (5-15%) while some folks prefer buffered formic acid to the HCl mixture), but that may be due to misunderstanding the formulation. No matter the technique, you should test to determine when it is complete (and make sure the samples are fixed properly before you start.) The timetable may be altered, but the end result should be the same. Remember - always add concentrated acids to water! Good luck, Lynne http://cshprotocols.cshlp.org/cgi/content/full/2008/6/pdb.rec11329?print=true Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.rec11329 Recipe Decalcifying solution 100 mL formic acid stock (88%) 80 mL HCl (concentrated) 820 mL H2O (distilled) ________________________________________ Caution Formic acid Formic acid HCOOH is highly toxic and extremely destructive to tissue of the mucous membranes, upper respiratory tract, eyes, and skin. It may be harmful by inhalation, ingestion, or skin absorption. Wear appropriate gloves and safety glasses (or face shield) and use in a chemical fume hood. ________________________________________ Caution HCl (Hydrochloric acid, Hydrochloride) HCl (hydrochloric acid, hydrochloride) is volatile and may be fatal if inhaled, ingested, or absorbed through the skin. It is extremely destructive to mucous membranes, upper respiratory tract, eyes, and skin. Wear appropriate gloves and safety glasses. Use with great care in a chemical fume hood. Wear goggles when handling large quantities. Also from the 2001 archives: From: Gayle Callis ________________________________ Sorry to disagree with disagreement. Interesting comments. Plus you can "overdecalcify' with any of the common acids, even formic - hence necessity for endpoint test. I never had problems with swelling using the formic acid/HCl mixture (Richman, Gelfand Hill, 1947 Arch Path 44:92. and have hundreds whole rat knee tissue sections to prove it. This was decalcifying method passed on to me from AFIP orthopedic histopathology aka bone and mineral laboratory years ago. I saw huge bones from human and animal decalcified with this mixture at their lab, and they were excellent preparations. The important thing is that the bone was COMPLETELY FIXED with NBF before any decalcification was started and xray tested to completion, and I never left knees (or other huge bones from bovine, sheep, goat or dog) in decalcifier overnight, particularly near endpoint. However, I may have reduced swelling since I rinse bone briefly, then immerse into either 70% ethanol or NBF to interupt the decalcification process near endpoint or at end of a week. A whole rat knee was usually decalcified in 2 days with the formic/HCl mixture. I have colleagues who used formic/HCl decalcifier before bone IHC controlled with testing. They also did not report swelling but did interupt decalcification near endpoint by reimmersion into NBF. Our technics were basically identical. As for immunostaining considerations, some argue that the faster calcium is removed by strong acids, the less damage there is to tissue antigens with exception of immunoglobulins. However, endpoint testing is necesary. I would be more inclined to stay with buffered formic acid. For mouse feet/ankles, rat feet/ankles, with many tiny bones involved and in my experience, they actually took longer to decalcify than whole femurs or tibias. The skin, toenails, connective tissues, and tight proximity of the bones seems to slow things down (that was a surprise!) and goes for fixation too. It was very evident that endpoint testing on feet/ankles samples is extremely important (xray saved the day!) or the final sections were poor, crunchy things. They also take some extended processing to have good infiltration of paraffin. The problems occuring with your bone preparation may be more than decalcification woes, it may be a combination of factors stemming from fixation, decalcification or processing - reevaluate carefully what has been done from fresh tissue to finished product. This is a At 08:47 AM 5/15/01 -0500, you wrote: >Jennifer >I prefer to use Kristensen's solution (1948). This is a mixture of >sodium formate formic acid. >340 gms sodium formate >1700 ml. 90% w/v formic acid. >Distilled water to 10 liters >can be made up and stored at RT for several months. >Need to use in a fume hood. >Need to have specimen well fixed before starting decalcification. >As Gayle pointed out this c an be used fro some IHC especially if >decalcifying at cold temperatures. > >For large specimens such as entire dog jaws can use a much lower >concentration with approximately 1-2% concentration of formic acid and >this allows some control. Need to stop maceration when carrying out >longer term decalcification by refixing in NBF. > >I cannot agree with Gayle re adding hydrochloric acid to a formic acid >mixture. While this may speed up the decalcification process, it has a >tendency to swell and as she pointed out to overdecalcify. Hydrochloric >acid alone has been used for some studies for IHC against some types of >collagen. >If you are looking for speed and routine histology can still use 1-5% >nitric acid with meticulous attention to end point. I prefer using >X-rays (if they are available to you). >Barry > > >Barry R. J. Rittman, Ph.D. >UTHHSC Dental Branch >6156 John Freeman avenue >Houston, TX. 77030 >713-500-4134 > >HOOVER_JENNIFER@LILLY.COM wrote: > >> >> Hello Histonetters! I promise, no more questions >> concerning Elmer's Glue!:-) My next quandry.......what kind of formic >> acid do you use for bone decalcification? I have looked in the Sigma >> catalog and there are many to choose from. Also is the final pH a >> concern or do you just prepare a 5% solution? I did consult my >> histology books but there isn't too much detail. Thank you for any >> suggestions! >> >> >> Jennifer Hoover >> Eli Lilly and Company > > > > Gayle Callis MT,HT,HTL(ASCP) Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman Bozeman MT 59717-3610 406 994-6367 404 994-4303 (FAX) -----Original Message----- Message: 1 Date: Wed, 8 Oct 2008 10:34:15 -0600 From: "Liz Chlipala" Subject: RE: [Histonet] Simple (?) equation question To: "jstaruk" , Message-ID: Content-Type: text/plain; charset="us-ascii" This is the way I would do it. Someone can correct me if I'm wrong. I consider the HCL to be 100% and the Formic acid to be just 88% at least that's the way we make up our formic acid for decal here (our stock formic acid is at 98%). You just need to use the simple equation V1xC1 = V2xC2 and perform two equations. Volume 1 - volume of stock reagent - this is what you want to figure out Volume 2 - volume of diluted reagent 100mls Concentration 1 - concentration of stock reagent - 88% formic acid ---- 100% HCL Concentration 2 - concentration of diluted reagent - 8% formic acid ----- 2% HCL FOR 2% HCL X x 100% = 100mls x 2% X = 2 mls of HCL acid FOR 8% Formic Acid X x 88% = 100mls x 8% X = 9.01 mls of Formic acid So you would add 2 mls of HCL and 9.01 mls of Formic Acid and 88.99 mls of dH20 (just subtracted from 100mls the final volume with the 2 amounts of acid to get 88.99) Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, October 08, 2008 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Simple (?) equation question Hello, I want to make a 2% HCl and 8% formic acid decalcifying solution. Since straight HCl is 37%, do I treat this as 100% (like you do using formalin), so the formula will simply be 98 water and 2 HCl or do I have to fraction it out so a 2% solution will actually be 95 water to 5 HCl? Also, the same question is for the formic acid, which is actually 88% My choices to make a 2% HCl and 8% formic acid solutions is either: A) 2 HCl, 8 formic acid and 90 water Or B) 5 HCl, 8.8 formic acid and 86.2 water Thanks Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 59, Issue 9 *************************************** ________________________________ The materials in this message are private and may contain Protected Healthcare Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. From max_histo_00 <@t> yahoo.it Thu Oct 9 01:22:07 2008 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Thu Oct 9 01:22:13 2008 Subject: R: [Histonet] Simple (?) equation question In-Reply-To: Message-ID: <120412.88060.qm@web23302.mail.ird.yahoo.com> A solution concentration is: Gr. of solute for 100 gr. of solution --- weight % Or: ml. of liquid solute for 100 ml of solution --- volume % (Ex.: H2O2, Alcohol) The first is the more used in chemistry. ? For HCl: 37 gr. HCl : 100 gr. Solution = 2 gr. HCl : X gr. Solution 5.4 gr. of Solution (37% HCl) contain 2 gr. of ?HCl Or: 5.4 ml. of Solution (37% HCl) --- > ?2 gr. of ?HCl ? For Formic Ac.: 88 gr. Formic Ac. : 100 gr. Solution = 8 gr. Formic Ac. : Y gr. Solution 9.1 gr. of Solution (88% Formic Ac.) contain 8 gr. of ?Formic Ac. Or: 9.1 ml. of Solution (88% Formic Ac.) --- > ?8 gr. of Formic Ac. ? Total solution = 5.4 + 9.1 = 14.5 ml. So I have to add 100 ? 14.5 = 85.5 ml. of H2O ? Best Regards, ? Massimo --- Mer 8/10/08, jstaruk ha scritto: Da: jstaruk Oggetto: [Histonet] Simple (?) equation question A: histonet@lists.utsouthwestern.edu Data: Mercoled? 8 ottobre 2008, 18:07 Hello, I want to make a 2% HCl and 8% formic acid decalcifying solution. Since straight HCl is 37%, do I treat this as 100% (like you do using formalin), so the formula will simply be 98 water and 2 HCl or do I have to fraction it out so a 2% solution will actually be 95 water to 5 HCl? Also, the same question is for the formic acid, which is actually 88% My choices to make a 2% HCl and 8% formic acid solutions is either: A) 2 HCl, 8 formic acid and 90 water Or B) 5 HCl, 8.8 formic acid and 86.2 water Thanks Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Scopri il blog di Yahoo! Mail: Trucchi, novit? e scrivi la tua opinione. http://www.ymailblogit.com/blog From lpwenk <@t> sbcglobal.net Thu Oct 9 04:50:08 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Oct 9 04:50:25 2008 Subject: [Histonet] Parts for LKB Message-ID: <000901c929f4$6a5a6d80$0202a8c0@HPPav2> If anyone (lab or vendor) has used parts for a ultramicrotome LKB-Nova, or has one that they want to get rid of very cheap (so we can use it for parts), please contact me via my work email pwenk@beaumont.edu Our LKB in Electron Microscopy has a broken part, we can't find a replacement, and we don't have the money to buy a new ultramicrotome. Any and all help would be appreciated. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 From annigyg <@t> gmail.com Thu Oct 9 07:02:16 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu Oct 9 07:02:25 2008 Subject: [Histonet] SV40 HRP protocol HELP Message-ID: Hello Histonetters please could someone out there (anyone) assist with a working protocol for Immuno SV40 (HRP) TIA abudhabiannie From burch007 <@t> mc.duke.edu Thu Oct 9 07:41:45 2008 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Thu Oct 9 07:41:57 2008 Subject: [Histonet] SV40 HRP protocol HELP In-Reply-To: Message-ID: Dear Anne: I use the SV40 (catalog # DP02) from Oncogene, a division of Calbiochem. A dilution of 1:500 consistently works well from lot to lot. For manual or autostainer platforms, pretreatment is with Dako's Target Retrieval pH 6.1 solution for 20 minutes in a 100 C water bath. Following a one hour primary incubation, the bound antibody is detected with HRP labeled Dako Envision Plus (30 minute incubation). I like using an enhanced DAB, such as Dako's DAB+. I find this SV40 also works well on the Leica Bond Max slide stainer. The same 1:500 dilution is used and the Bond pretreatment is programmed for 20 minutes with HIER solution #1 (aka ER1). Other than the pretreatment programming, their standard incubation schedule for all of the subsequent steps works well. Best Regards Jim Burchette http://www.emdbiosciences.com/Products/ProductDisplay.asp?catno=DP02& Catalog # DP02 Host: Mouse Isotype: IgG2a Immunogen: purified SV40 large T-antigen Epitope: Within amino acids 83-128 Form: Liquid Formulation: In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5. Preservative: ?0.1% sodium azide Positive Control: SV80 or SV-T2 cells Negative Control: 3T3 cells Comments: Recognizes ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells. Ref.: DeCaprio, J.A., et al. 1988. Cell 54, 275. Whyte, P., et al. 1988. Nature 334, 124. Mitchell, P.J., et al. 1987. Cell 50, 847. Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001. Simanis, V. and Lane, D.P. 1985. Virol. 144, 88. Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379. Sarnow, P., et al. 1982. Cell 28, 387. Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41. Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261. Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754. Tooze, J. 1973. Cold Spring Harbor, New York. Tegtmeyer, P. 1972. J. Virol. 10, 591. "Anne van Binsbergen" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/09/2008 08:05 AM To histonet , histonet-bounces@lists.utsouthwestern.edu cc Subject [Histonet] SV40 HRP protocol HELP Hello Histonetters please could someone out there (anyone) assist with a working protocol for Immuno SV40 (HRP) TIA abudhabiannie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Oct 9 07:42:35 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 9 07:42:39 2008 Subject: [Histonet] citrisolve? In-Reply-To: <48ED2820.3060801@ucsd.edu> Message-ID: <534207.60593.qm@web65705.mail.ac4.yahoo.com> Nicole: CitriSolve works somewhat better than other d-Limonene?xylene substitutes because it is a hybrid. It contains d-Limonene at 70% (or 50% according with other recipes)?+ quaternary amnine mixture (or aliphatic hydrocarbons in another recipe) at 25% (or greater %) and 2-butoxyethanol at 5%. This?2-butoxyethanol is a suspected carcinogen with a TWA of only 25 ppm. CitriSolve is described as corrosive to the eyes. It costs 2.31 times?more than?Xylene and I personally would not use it. Ren? J.? --- On Wed, 10/8/08, kbowden wrote: From: kbowden Subject: Re: [Histonet] citrisolve? To: "Patten, Nicole (NIH/NIAAA) [F]" Cc: histonet@lists.utsouthwestern.edu Date: Wednesday, October 8, 2008, 5:37 PM I have used Citrisolve for serveral years. I don't think anything compares to Xylene, but I do think this comes very close. I change it about once a week. It pretty much deparaffinizes in the same amount of time. Disposal is dependent on the regulations in your area. When I started using it our Environmental Health and Safety said pour it down the drain, but as time goes by and with more regulations now I have to collect it and have it removed with hazardous waste. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. Patten, Nicole (NIH/NIAAA) [F] wrote: > Does anyone have any experience using Citrisolve as a Xylene alternative > to deparaffinize tissue sections? Do I use it just like Xylene for the > same amount of time? Can it be reused or should I use new Citrisolve > each time? How do I dispose of it? > > > > Any help at all would be much appreciated. Thanks! > > > > Nicole J. Patten > Post-Baccalaureate Fellow/IRTA > National Institutes of Health > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kynemitz <@t> travmax.com Thu Oct 9 07:45:06 2008 From: kynemitz <@t> travmax.com (Kyla Nemitz) Date: Thu Oct 9 07:45:42 2008 Subject: [Histonet] PERM Grossing Supervisor position in Austin, TX Message-ID: <9416D9FA37C1C04FA83D69684E95D2E71E4DE3CE@exbk2.maxhealth.com> Hello All - A facility I work closely with in Austin, TX has asked me to help recruit for their Grossing Supervisor opening. They have an immediate opening for the second shift. Candidates considered would be an experienced Gross tech, histotech or PA with pathology lab experience and strong leadership qualities. 2 year degree with science required and a 4 year degree preferred. Feel free to forward my information to anyone possibly interested in this position or any other openings I currently have. I recruit for travel, temp-to-perm and perm laboratory modalities. Thank you in advance and have a great day! Kyla Nemitz Recruitment Manager TravelMax Medical Professionals - Maxim Healthcare Tel: 888.800.1855 or 813.371.5175 Fax: 800.294.1248 Search our jobs or apply online at: www.TravelMaxAllied.com From alexandra.meinl <@t> gmail.com Thu Oct 9 08:17:07 2008 From: alexandra.meinl <@t> gmail.com (Alexandra Meinl) Date: Thu Oct 9 08:17:11 2008 Subject: [Histonet] Decalcification prior to Alizarine red or von Kossa possible? Message-ID: Hello all, I have to stain some specimens with bone formation in soft tissue. They want me to do Alizarine red or Kossa stain. But there are some heavily mineralized nodules in the tissue, I think they can't be cut nicely without some kind of decalcification. How should I treat these samples? Is there a way to decalcify them without negative effects on the calcium stain? Thanks in advance! Alexandra From CHRISH <@t> HEALTHCARESCOUTS.COM Thu Oct 9 08:34:46 2008 From: CHRISH <@t> HEALTHCARESCOUTS.COM (Chris Handrahan) Date: Thu Oct 9 08:41:57 2008 Subject: [Histonet] Discover new career opportunities with Healthcare Scouts and LinkedIn Message-ID: Healthcare Scouts, the nation's premier recruiting firm specializing in permanent placement of licensed healthcare professionals invites you to join and be active participants in our Linked In groups Allied Health Professionals - http://www.linkedin.com/groups?gid=858727 Laboratory and Bio Tech Professionals - http://www.linkedin.com/groups?gid=749287 With our experienced staff and vast network of industry contacts, we are the right partner for your career search. We strive to help individuals discover new career opportunities and explore career options that include better quality of life, more money, better schedule, and more challenge. Please don't hesitate to call or email me your resume today to get what you deserve!! Chris Handrahan Managing Director of Allied Health Healthcare Scouts 800-708-0605 office 321-231-5427 cell chrish@healthcarescouts.com www.healthcarescouts.com From jdooley2008 <@t> yahoo.com Thu Oct 9 09:16:31 2008 From: jdooley2008 <@t> yahoo.com (James Dooley) Date: Thu Oct 9 09:16:36 2008 Subject: [Histonet] Toluene vs. Xylene Message-ID: <527229.12110.qm@web45910.mail.sp1.yahoo.com> Is there a significant difference between using xylene and toluene when deparaffinizing embedding tissue? Thank you, James From rjbuesa <@t> yahoo.com Thu Oct 9 09:38:19 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 9 09:38:23 2008 Subject: [Histonet] Toluene vs. Xylene In-Reply-To: <527229.12110.qm@web45910.mail.sp1.yahoo.com> Message-ID: <571831.37907.qm@web65709.mail.ac4.yahoo.com> Not really and both are as dangerous and both should be avoided. As a matter of fact xylene began to be used in the mid 1950s to substitute chemicals like toluene, until it was found that both were equally dangerous. Toluene is present in many mounting media. Ren? J. --- On Thu, 10/9/08, James Dooley wrote: From: James Dooley Subject: [Histonet] Toluene vs. Xylene To: histonet@lists.utsouthwestern.edu Date: Thursday, October 9, 2008, 10:16 AM Is there a significant difference between using xylene and toluene when deparaffinizing embedding tissue? Thank you, James _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Thu Oct 9 10:03:28 2008 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Oct 9 10:03:40 2008 Subject: [Histonet] Decalcification prior to Alizarine red or von Kossa possible? In-Reply-To: References: Message-ID: Alexandra, It sounds like to me that you might be working with some sort of calcium phosphate material in the presence of demineralized bone and/or soft tissue in say a muscle pocket and looking for the presence/absence of bone formation. If you decal the tissue you will lose the ability to detect any clear mineralized bone as the alizarin red and Von Kossa reaction both identify mineralization (calcium). Also, if there are heavily mineralized nodules as you indicate, then I would say you do not have the option to cut as a paraffin block. If I was doing this project, I would embed the tissue in methyl methacrylate (MMA), cut thin sections (4-6 microns) on a motorized rotary microtome with a tungsten-carbide knife, deplastify the tissue sections (similar to deparaffinization), and stain with the Von Kossa reaction and MacNeal's tetrachrome stain. This combination would then clearly yield the presence or absence of mineralization where the increasing degree of mineralization (dense older bone) would be seen as black and any newly formed mineralization (vital bone - osteoid) would be grayish-green. Furthermore, this staining combination will also allow for the identification of bone forming (osteoblasts/blue) and bone resorbing (osteoclasts/bluish-green) cells. In fact, this stain will clearly identify any active mineralization (osteoid) that has formed (osteoid) and show the active bone forming osteoblasts lining the surface as nice plump (single nuclei) cuboidal cells. Additionally, if all you care about is mineralization or newly formed bone, you could also perform a Goldner's trichrome stain where any mineralization or newly formed bone (osteoid) will be highlighted as red. Hope this helps and let me know if you need any further assistance or advice. Best Regards, Jack > Date: Thu, 9 Oct 2008 15:17:07 +0200> From: alexandra.meinl@gmail.com> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Decalcification prior to Alizarine red or von Kossa possible?> > Hello all,> > I have to stain some specimens with bone formation in soft tissue. They want> me to do Alizarine red or Kossa stain.> But there are some heavily mineralized nodules in the tissue, I think they> can't be cut nicely without some kind of decalcification.> How should I treat these samples? Is there a way to decalcify them without> negative effects on the calcium stain?> > Thanks in advance!> > Alexandra> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ See how Windows Mobile brings your life together?at home, work, or on the go. http://clk.atdmt.com/MRT/go/msnnkwxp1020093182mrt/direct/01/ From SEsparza <@t> seton.org Thu Oct 9 11:20:43 2008 From: SEsparza <@t> seton.org (Esparza, Sandra) Date: Thu Oct 9 11:20:47 2008 Subject: [Histonet] Any experience with the Mohs "cryo embedder" Message-ID: <3D79F47DC92B204F9E5D35C885DFC5CB01003C01@AUSEX2VS1.seton.org> Hi Everyone, I am new to the Mohs technique for dermatology specimens and am trying to find out if anyone has used the Cryo Embedder system. It sound great but I don't want to order it until I'm sure that it is user friendly. Any help with the Mohs technique would be greatly appreciated. Thanks, Sandra HT (ASCP) Dell Children's Hospital Austin, Texas From kenneth.a.troutman <@t> Vanderbilt.Edu Thu Oct 9 12:29:15 2008 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Thu Oct 9 12:29:23 2008 Subject: Subject: [Histonet] SV40 HRP protocol HELP Message-ID: <37DEF9AF72994947AF693956A59B9B660127FFCC@mailbe03.mc.vanderbilt.edu> This is our current protocol (on Dako Autostainer): 1. Pretreat slides in Trilogy (Cell Marque) in pressure cooker for 20 minutes. 2. Cool in hot soln for 10 minutes. 3. Rinse in DI H2O. 4. Peroxidase block for 5 min. 5. Protein block for 5 min. 6. SV-40 primary (Chemicon 1:10,000) for 30 min @ RT 7. Dako Envision Dual link polymer for 30 min @ RT 8. DAB 5 min. 9. Counterstain, dehydrate, clear and coverslip. Hope this helps. Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN From Jackie.O'Connor <@t> abbott.com Thu Oct 9 12:44:38 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Oct 9 12:45:02 2008 Subject: [Histonet] Toluene vs. Xylene In-Reply-To: <571831.37907.qm@web65709.mail.ac4.yahoo.com> Message-ID: Yeah - but since Toluene is the major component of airplane glue- at least you'll be happier using toluene over xylene. Jackie Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 10/09/2008 09:38 AM Please respond to rjbuesa@yahoo.com To histonet@lists.utsouthwestern.edu, James Dooley cc Subject Re: [Histonet] Toluene vs. Xylene Not really and both are as dangerous and both should be avoided. As a matter of fact xylene began to be used in the mid 1950s to substitute chemicals like toluene, until it was found that both were equally dangerous. Toluene is present in many mounting media. Ren? J. --- On Thu, 10/9/08, James Dooley wrote: From: James Dooley Subject: [Histonet] Toluene vs. Xylene To: histonet@lists.utsouthwestern.edu Date: Thursday, October 9, 2008, 10:16 AM Is there a significant difference between using xylene and toluene when deparaffinizing embedding tissue? Thank you, James _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LINDA.MARGRAF <@t> childrens.com Thu Oct 9 12:49:51 2008 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Thu Oct 9 12:50:13 2008 Subject: [Histonet] section thickness Message-ID: <48EDFDEF.F783.00DA.0@childrens.com> Histonetters: Here's a query from Janet that she couldn't get to post to the list. Please respond to the list so she'll see the response. Thanks. I would like to poll the group regarding routine practices for thickness of paraffin sections. I am particularly interested in brain, spinal cord, lymph node, tonsil, and renal biopsy Most references range from 3-6 um for routine tissues with 4-5um being most prevalent. What do you use? Which tissues do you cut at a different thickness routinely? I am not referring to special stains as the protocol would prescribe the thickness. Thanks for your help. Janet Keeping A.R.T., B. Voc. Ed Instructor Medical Laboratory Sciences Please consider the environment before printing this e-mail

This e-mail, facsimile, or letter and any files or attachments transmitted with it contains
information that is confidential and privileged. This information is intended only for the use of the
individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further
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disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
violation of federal or state law and regulations. If you have received this information in error,
please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at
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From JWeems <@t> sjha.org Thu Oct 9 12:55:28 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Oct 9 12:55:47 2008 Subject: [Histonet] section thickness In-Reply-To: <48EDFDEF.F783.00DA.0@childrens.com> References: <48EDFDEF.F783.00DA.0@childrens.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA509EF57@ITSSSXM01V6.one.ads.che.org> We routinely cut at 4 um, 3 um for lymph nodes, bone marrows, and needle biopsies. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Thursday, October 09, 2008 1:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] section thickness Histonetters: Here's a query from Janet that she couldn't get to post to the list. Please respond to the list so she'll see the response. Thanks. I would like to poll the group regarding routine practices for thickness of paraffin sections. I am particularly interested in brain, spinal cord, lymph node, tonsil, and renal biopsy Most references range from 3-6 um for routine tissues with 4-5um being most prevalent. What do you use? Which tissues do you cut at a different thickness routinely? I am not referring to special stains as the protocol would prescribe the thickness. Thanks for your help. Janet Keeping A.R.T., B. Voc. Ed Instructor Medical Laboratory Sciences Please consider the environment before printing this e-mail

This e-mail, facsimile, or letter and any files or attachments transmitted with it contains
information that is confidential and privileged. This information is intended only for the use of the
individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further
disclosures are prohibited without proper authorization. If you are not the intended recipient, any
disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
violation of federal or state law and regulations. If you have received this information in error,
please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at
privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all
applicable privileges related to this information.


_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From jdooley2008 <@t> yahoo.com Thu Oct 9 13:03:30 2008 From: jdooley2008 <@t> yahoo.com (James Dooley) Date: Thu Oct 9 13:03:38 2008 Subject: [Histonet] old paraffin Message-ID: <90772.26793.qm@web45914.mail.sp1.yahoo.com> We have bags of paraffin that are about 3 years old in the lab. I was wondering if it is still good for embedding tissue. James From arsenn <@t> hsh.org Thu Oct 9 13:31:35 2008 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Thu Oct 9 13:31:44 2008 Subject: [Histonet] Waste - OP Message-ID: As always, everyone has been a wealth of information!!! Thank you to everyone who responded to my question about the waste on our Benchmark XT !! I have a lot of ammo now and it's really helping us! Have a fantastic weekend all! Amy Senn, Camp Hill PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From rjbuesa <@t> yahoo.com Thu Oct 9 13:55:40 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 9 13:55:43 2008 Subject: [Histonet] old paraffin In-Reply-To: <90772.26793.qm@web45914.mail.sp1.yahoo.com> Message-ID: <33371.82228.qm@web65705.mail.ac4.yahoo.com> Most likely they will, specially if they are in closed bags. I have used much older paraffin (up to 7 years). Ren? J. --- On Thu, 10/9/08, James Dooley wrote: From: James Dooley Subject: [Histonet] old paraffin To: histonet@lists.utsouthwestern.edu Date: Thursday, October 9, 2008, 2:03 PM We have bags of paraffin that are about 3 years old in the lab. I was wondering if it is still good for embedding tissue. James _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Oct 9 13:55:38 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Oct 9 13:55:46 2008 Subject: [Histonet] lean histology Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82E69@EMAIL.archildrens.org> I want to hear from labs who have actually converted to the lean process. I want to know what changes you made, if you purchased new equipment to facilitate the lean process and have your work hours changed. Thanks! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From rjbuesa <@t> yahoo.com Thu Oct 9 13:57:00 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Oct 9 13:57:04 2008 Subject: [Histonet] section thickness In-Reply-To: <48EDFDEF.F783.00DA.0@childrens.com> Message-ID: <595628.70425.qm@web65712.mail.ac4.yahoo.com> General routine = 5 ?m Brain = at least 8 ?m Ren? J. --- On Thu, 10/9/08, LINDA MARGRAF wrote: From: LINDA MARGRAF Subject: [Histonet] section thickness To: histonet@lists.utsouthwestern.edu Date: Thursday, October 9, 2008, 1:49 PM Histonetters: Here's a query from Janet that she couldn't get to post to the list. Please respond to the list so she'll see the response. Thanks. I would like to poll the group regarding routine practices for thickness of paraffin sections. I am particularly interested in brain, spinal cord, lymph node, tonsil, and renal biopsy Most references range from 3-6 um for routine tissues with 4-5um being most prevalent. What do you use? Which tissues do you cut at a different thickness routinely? I am not referring to special stains as the protocol would prescribe the thickness. Thanks for your help. Janet Keeping A.R.T., B. Voc. Ed Instructor Medical Laboratory Sciences Please consider the environment before printing this e-mail

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_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Thu Oct 9 14:02:20 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Thu Oct 9 14:03:28 2008 Subject: [Histonet] Any experience with the Mohs "cryo embedder" References: <3D79F47DC92B204F9E5D35C885DFC5CB01003C01@AUSEX2VS1.seton.org> Message-ID: <08A0A863637F1349BBFD83A96B27A50A12017B@uwhis-xchng3.uwhis.hosp.wisc.edu> Sandra: Who is selling this, and how does it work? I have been testing one type of embedder, but I know there are several out there. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Esparza, Sandra Sent: Thu 10/9/2008 11:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Any experience with the Mohs "cryo embedder" Hi Everyone, I am new to the Mohs technique for dermatology specimens and am trying to find out if anyone has used the Cryo Embedder system. It sound great but I don't want to order it until I'm sure that it is user friendly. Any help with the Mohs technique would be greatly appreciated. Thanks, Sandra HT (ASCP) Dell Children's Hospital Austin, Texas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Thu Oct 9 14:45:14 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Oct 9 14:45:21 2008 Subject: [Histonet] Any experience with the Mohs "cryo embedder" In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A12017B@uwhis-xchng3.uwhis.hosp.wisc.edu> References: <3D79F47DC92B204F9E5D35C885DFC5CB01003C01@AUSEX2VS1.seton.org> <08A0A863637F1349BBFD83A96B27A50A12017B@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <5b6eb13e0810091245u20a311cbld148b1e319411d@mail.gmail.com> Does anyone else freeze the tissue to a glass slide to get it embedded flat? That's how I've been show and it seems to work well. I've used plastic embedding molds too, but they don't always give me a flat surface. Mark On Thu, Oct 9, 2008 at 12:02 PM, Ingles Claire wrote: > Sandra: > Who is selling this, and how does it work? I have been testing one type of > embedder, but I know there are several out there. > Claire > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Esparza, > Sandra > Sent: Thu 10/9/2008 11:20 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Any experience with the Mohs "cryo embedder" > > > > Hi Everyone, > > > > I am new to the Mohs technique for dermatology specimens and am trying > to find out if anyone has used the Cryo Embedder system. It sound great > but I don't want to order it until I'm sure that it is user friendly. > Any help with the Mohs technique would be greatly appreciated. Thanks, > > > Sandra HT (ASCP) > > Dell Children's Hospital > > Austin, Texas > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From azdudley <@t> hotmail.com Thu Oct 9 15:00:57 2008 From: azdudley <@t> hotmail.com (anita dudley) Date: Thu Oct 9 15:01:04 2008 Subject: [Histonet] out dated reagents Message-ID: to all!!! thanks so much for your responses to my question about outdated reagents, that helped me, cause I think it is such a waste for throw everything out, I am going to show them to my director. amy, our cytolotist go to the FNA's and they label everything at the bedside. the path only goes when the cytotech has all the slides ready for them to look at. that is all written in the cyto dept procedure for FNA. hope this helps, anything else just ask. thanks again all, have a good weekend, anita> Subject: RE: [Histonet] out dated reagents> Date: Tue, 7 Oct 2008 15:38:48 -0400> From: ASelf@georgetownhospitalsystem.org> To: azdudley@hotmail.com> > Anita,> Our inspection is any day now and I have cone across a checklist> question that has stumbled me. Do your pathologist do FNA procedures?> And if so do you have anything that you could share with me regarding> this question from the CAP checklist below: If the pathologist> performs FNA procedures, is there a documented procedure to prevent> errors in the identification of the patient, the site and the procedure?> > > Any info or help would be greatly appreciated. > > Thanks amy> > > > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita> dudley> Sent: Monday, October 06, 2008 12:19 PM> To: Histonet@lists.utsouthwestern.edu> Subject: [Histonet] out dated reagents> > hello all, we were inspected the beginning of june by cap and one of> our deficiency was anp.21382. it had to do with outdated reagents. my> director want all of my dry chemicals gone!!!! I have gone through> them and just have what I need for the special stains that we do in> house. does anyone know do they put expiration dates on dry chems. we> have things like light green, biebrich scarlet, periodic acid. I really> hate to get rid of all of this but he thinks I should.> > what are others doing about this? I can see after you have made up> things to not keep it long but dry I know many yrs ago the biological> stain commission thought it would be meaningless and arbitrary to put> expiration dates on stains. it would me more important to maintain a> record of the use of the powdered stain. which is what we do when we do> the stain with our controls.> > anyway just wondering if there were any thoughts out there about this. > > anita dudley> providence hosp> mobile alabama> _________________________________________________________________> See how Windows Mobile brings your life together-at home, work, or on> the go.> http://clk.atdmt.com/MRT/go/msnnkwxp1020093182mrt/direct/01/____________> ___________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> NOTE:> The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer.> Thank you.> _________________________________________________________________ Stay up to date on your PC, the Web, and your mobile phone with Windows Live. http://clk.atdmt.com/MRT/go/msnnkwxp1020093185mrt/direct/01/ From azdudley <@t> hotmail.com Thu Oct 9 15:03:35 2008 From: azdudley <@t> hotmail.com (anita dudley) Date: Thu Oct 9 15:03:41 2008 Subject: [Histonet] (no subject) Message-ID: boy sorry guys, that last message sure doesn't look right. don't know what I did. amy if you can't make it out let me know. anita _________________________________________________________________ See how Windows Mobile brings your life together?at home, work, or on the go. http://clk.atdmt.com/MRT/go/msnnkwxp1020093182mrt/direct/01/ From cmmathis1 <@t> bellsouth.net Thu Oct 9 15:16:53 2008 From: cmmathis1 <@t> bellsouth.net (cmmathis1@bellsouth.net) Date: Thu Oct 9 15:16:59 2008 Subject: [Histonet] Heat and autofluorescence Message-ID: <100920082016.13660.48EE66B5000B54A40000355C22230647629B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> I have a researcher in my lab that transfected cells with EGFP, injected them into mouse and then 3 weeks later harvested the tissue and frozen it in OCT as usual. After sectioning, he air dried the slides for 1 hour and then dried them at 60 degrees C for 1 hour. He was hoping just to see his EGFP infected cells which are bright green, however, the autofluorescence is everywhere - both with GFP and Rhodamine filters. Our question is - did the heating in the oven cause increased autofluorescence? Cathy From leiker <@t> buffalo.edu Thu Oct 9 15:42:31 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Thu Oct 9 15:42:44 2008 Subject: [Histonet] Heat and autofluorescence In-Reply-To: <100920082016.13660.48EE66B5000B54A40000355C22230647629B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> References: <100920082016.13660.48EE66B5000B54A40000355C22230647629B0A02D208 9B9A019C04040A0DBFCE9C07089B0E03030C@att.net> Message-ID: I would say no, since it's routine to dry them at 60 degrees 1 hour. What tissue was this? What was it fixed in? We've had similar autofluorescence issues doing exactly the same thing your researcher is doing with EGFP. We were looking at skeletal muscle, however, which is highly autofluorescent in just about every visible light channel. EGFP-containing cells/tissues need to be fixed in 4% PFA. Avoid 70% ethanol as it will allow this protein to leak out. We had a hard time in general with EGFP. Merced --On Thursday, October 09, 2008 8:16 PM +0000 cmmathis1@bellsouth.net wrote: > I have a researcher in my lab that transfected cells with EGFP, injected > them into mouse and then 3 weeks later harvested the tissue and frozen it > in OCT as usual. After sectioning, he air dried the slides for 1 hour > and then dried them at 60 degrees C for 1 hour. He was hoping just to > see his EGFP infected cells which are bright green, however, the > autofluorescence is everywhere - both with GFP and Rhodamine filters. > Our question is - did the heating in the oven cause increased > autofluorescence? > > Cathy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 In order to put yourself in someone else's shoes, you must first take off yours. From leiker <@t> buffalo.edu Thu Oct 9 16:03:25 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Thu Oct 9 16:03:31 2008 Subject: [Histonet] Heat and autofluorescence In-Reply-To: <100920082016.13660.48EE66B5000B54A40000355C22230647629B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> References: <100920082016.13660.48EE66B5000B54A40000355C22230647629B0A02D208 9B9A019C04040A0DBFCE9C07089B0E03030C@att.net> Message-ID: <9783C1674804287BC7B3809E@bchwxp2702.ad.med.buffalo.edu> I just realized - these are frozen sections! I am getting my frozens and paraffins crossed here, sorry! Don't heat them in that case - that would be disaster! Merced --On Thursday, October 09, 2008 8:16 PM +0000 cmmathis1@bellsouth.net wrote: > I have a researcher in my lab that transfected cells with EGFP, injected > them into mouse and then 3 weeks later harvested the tissue and frozen it > in OCT as usual. After sectioning, he air dried the slides for 1 hour > and then dried them at 60 degrees C for 1 hour. He was hoping just to > see his EGFP infected cells which are bright green, however, the > autofluorescence is everywhere - both with GFP and Rhodamine filters. > Our question is - did the heating in the oven cause increased > autofluorescence? > > Cathy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 In order to put yourself in someone else's shoes, you must first take off yours. From leiker <@t> buffalo.edu Thu Oct 9 16:19:54 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Thu Oct 9 16:19:58 2008 Subject: [Histonet] Manual Microwave Method of Tissue Processing In-Reply-To: <9783C1674804287BC7B3809E@bchwxp2702.ad.med.buffalo.edu> References: <100920082016.13660.48EE66B5000B54A40000355C22230647629B0A02D208 9B9A019C04040A0DBFCE9C07089B0E03030C@att.net> <9783C1674804287BC7B3809E@bchwxp2702.ad.med.buffalo.edu> Message-ID: <483C4EA5F30EA4D1BB8620B6@bchwxp2702.ad.med.buffalo.edu> What are people's exerience with manual processing of FFPE tissues using a microwave? My boss has me looking into a manual method of processing tissues to cut costs - small lab with small budget getting smaller all the time... Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 In order to put yourself in someone else's shoes, you must first take off yours. From mickie25 <@t> netzero.net Thu Oct 9 16:49:38 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Thu Oct 9 17:04:36 2008 Subject: [Histonet] Any experience with the Mohs "cryo embedder" In-Reply-To: <5b6eb13e0810091245u20a311cbld148b1e319411d@mail.gmail.com> References: <3D79F47DC92B204F9E5D35C885DFC5CB01003C01@AUSEX2VS1.seton.org> <08A0A863637F1349BBFD83A96B27A50A12017B@uwhis-xchng3.uwhis.hosp.wisc.edu> <5b6eb13e0810091245u20a311cbld148b1e319411d@mail.gmail.com> Message-ID: Hi Mark, Thanks for your input on how the Leica CM 1100 is working for you. Regarding the best way to embed tissues for Mohs. Yes, the slide method is the most accurate to get the tissue flat as long as the epidermis is relaxed so it lays down on the glass too. Watch out for trapped air bubbles under the specimen. I smear a thin coat of O.C.T. on the slide and this helps hold the epi down during embedding. Does the Leica 1100 allow you to reposition the block face parallel to the knife when you embed? That is important so that you don't cut any more tissue away than necessary to get a full section of epidermis and deep margin. There is also a device which is very helpful in embedding Mohs specimens to minimize the need to line up the block face to the knife. Many labs use it. I carry one where ever I go to help me embed fast and accurately. If you would like to know more give me a call. Good Luck! Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, October 09, 2008 12:45 PM To: Ingles Claire Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Any experience with the Mohs "cryo embedder" Does anyone else freeze the tissue to a glass slide to get it embedded flat? That's how I've been show and it seems to work well. I've used plastic embedding molds too, but they don't always give me a flat surface. Mark On Thu, Oct 9, 2008 at 12:02 PM, Ingles Claire wrote: > Sandra: > Who is selling this, and how does it work? I have been testing one type of > embedder, but I know there are several out there. > Claire > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Esparza, > Sandra > Sent: Thu 10/9/2008 11:20 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Any experience with the Mohs "cryo embedder" > > > > Hi Everyone, > > > > I am new to the Mohs technique for dermatology specimens and am trying > to find out if anyone has used the Cryo Embedder system. It sound great > but I don't want to order it until I'm sure that it is user friendly. > Any help with the Mohs technique would be greatly appreciated. Thanks, > > > Sandra HT (ASCP) > > Dell Children's Hospital > > Austin, Texas > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.7.6/1714 - Release Date: 10/8/2008 7:01 AM From gideon <@t> BATTELLE.ORG Thu Oct 9 19:10:27 2008 From: gideon <@t> BATTELLE.ORG (Gideon, Katherine M) Date: Thu Oct 9 19:10:40 2008 Subject: [Histonet] information on 15 mL or 50 mL siliconized conical tubes Message-ID: For collecting Bronchoalveolar lavage fluid from small rodents we use 2.0 mL siliconized micro-centrifuge tubes for the first two washes - we need larger tubes for the remaining four washes. Has anybody ever found siliconized tubes in 5.0 mL, 15 mL and 50 mL size? I have searched for sometime and unable to locate in catalogs nor on-line. I realize we could make our own but would like to find supplier for ready-made tubes. Any help would be appreciated. Thank you. Katherine M. Gideon Pathology & Toxicology Manager (509) 375-2972 (phone) (509) 521-0743 (cell) (614) 458-0248 (fax) From billions1998 <@t> hotmail.com Thu Oct 9 19:44:23 2008 From: billions1998 <@t> hotmail.com (Sinoera Tech) Date: Thu Oct 9 20:17:17 2008 Subject: [Histonet] Alcian Yellow In-Reply-To: References: Message-ID: Dear Sirs, We are the manufacturer of Alcian Yellow. Kind Regards. - Minggeng Wang, Ph.D / President SUZHOU SINOERA CHEM CO., LTD. 125 Binhe Road Suzhou New & Hi-Tech District 215011 China Fax: +86 512 68258994 Tel: +86 512 68246939 http://www.sinoeratech.com From cooperms <@t> mail.nih.gov Thu Oct 9 20:38:20 2008 From: cooperms <@t> mail.nih.gov (Cooperman, Sharon (NIH/NICHD) [E]) Date: Thu Oct 9 20:38:24 2008 Subject: [Histonet] having problems with histonet Message-ID: <2B184EA4ADDB0D41845D8B3730B10F651DA980@NIHCESMLBX7.nih.gov> Hi, I'm having problems with the histonet. It doesn't recognize me as a subscriber and when I tried to subscribe with a new email address I got the following message on replying to the confirmation email: X-IronPortListener: CES-Inbound X-SBRS: 3.3 X-IronPort-Anti-Spam-Filtered: true X-IronPort-Anti-Spam-Result: AgYBALtM7kjHpZiihWdsb2JhbAALkkmBBQEBAQoHBAoHEQWJN5pHhh08gWo Subject: The results of your email commands From: histonet-bounces@lists.utsouthwestern.edu To: cooperms@mail.nih.gov Date: Thu, 09 Oct 2008 20:30:30 -0500 X-BeenThere: histonet@lists.utsouthwestern.edu List-Id: For the exchange of information pertaining to histotechnology and related fields Sender: histonet-bounces@lists.utsouthwestern.edu X-Scan-Signature: 2afe1b2206e4d1abd6c168b829df7099 X-SA-Exim-Connect-IP: 127.0.0.1 X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to false The results of your email command are provided below. Attached is your original message. - Results: Ignoring non-text/plain MIME parts Invalid confirmation string. Note that confirmation strings expire approximately 3 days after the initial subscription request. If your confirmation has expired, please try to re-submit your original request or message. - Unprocessed: -----Original Message----- From: histonet-request@lists.utsouthwestern.edu = [mailto:histonet-request@lists.utsouthwestern.edu] Sent: Thu 10/9/2008 9:26 PM To: Cooperman, Sharon (NIH/NICHD) [E] Subject: confirm 0e4978ec2aad5bf6fb354d81c2c8deb859bb9d96 =20 Mailing list subscription confirmation notice for mailing list Histonet We have received a request from 128.231.88.4 for subscription of your email address, "cooperms@mail.nih.gov", to the histonet@lists.utsouthwestern.edu mailing list. To confirm that you want to be added to this mailing list, simply reply to this message, keeping the Subject: header intact. 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Received: from [199.242.236.161] (helo=swlx161.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1Ko6pq-00077x-3z for histonet-request@lists.utsouthwestern.edu; Thu, 09 Oct 2008 20:30:30 -0500 Received: from nihxway2out.hub.nih.gov ([128.231.90.110]) by swlx161.swmed.edu with esmtp (Exim 4.69) (envelope-from ) id 1Ko6po-0000V2-RD for histonet-request@lists.utsouthwestern.edu; Thu, 09 Oct 2008 20:30:30 -0500 X-IronPortListener: Outbound_SMTP Received: from nihcessmtp.hub.nih.gov ([128.231.90.115]) by nihxway2out.hub.nih.gov with ESMTP; 09 Oct 2008 21:30:28 -0400 Received: from NIHCESMLBX7.nih.gov ([156.40.71.207]) by NIHCESSMTP.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.3959); Thu, 9 Oct 2008 21:30:27 -0400 X-MimeOLE: Produced By Microsoft Exchange V6.5 Content-class: urn:content-classes:message MIME-Version: 1.0 Content-Type: multipart/mixed; boundary="----_=_NextPart_001_01C92A77.C66CED12" Date: Thu, 9 Oct 2008 21:30:22 -0400 Message-ID: <2B184EA4ADDB0D41845D8B3730B10F651DA97C@NIHCESMLBX7.nih.gov> X-MS-Has-Attach: X-MS-TNEF-Correlator: <2B184EA4ADDB0D41845D8B3730B10F651DA97C@NIHCESMLBX7.nih.gov> Thread-Topic: confirm 0e4978ec2aad5bf6fb354d81c2c8deb859bb9d96 Thread-Index: AckqdzDS5/6borvCR4m02ZfSqaM2DgAAJKa7 References: From: "Cooperman, Sharon (NIH/NICHD) [E]" To: X-OriginalArrivalTime: 10 Oct 2008 01:30:27.0754 (UTC) FILETIME=[C68F88A0:01C92A77] X-Scan-Signature: 30c46b6f917c73ddc12a27b699747596 X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on swlx161.swmed.edu X-Spam-Level: X-Spam-Status: No, score=-4.0 required=5.0 tests=RCVD_IN_DNSWL_MED,SPF_PASS autolearn=disabled version=3.2.5 X-Spam-Relay-Country: US US US Subject: RE: confirm 0e4978ec2aad5bf6fb354d81c2c8deb859bb9d96 X-SA-Exim-Connect-IP: 199.242.236.161 X-SA-Exim-Mail-From: cooperms@mail.nih.gov X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to false -----Original Message----- From: histonet-request@lists.utsouthwestern.edu [mailto:histonet-request@lists.utsouthwestern.edu] Sent: Thu 10/9/2008 9:26 PM To: Cooperman, Sharon (NIH/NICHD) [E] Subject: confirm 0e4978ec2aad5bf6fb354d81c2c8deb859bb9d96 Mailing list subscription confirmation notice for mailing list Histonet We have received a request from 128.231.88.4 for subscription of your email address, "cooperms@mail.nih.gov", to the histonet@lists.utsouthwestern.edu mailing list. To confirm that you want to be added to this mailing list, simply reply to this message, keeping the Subject: header intact. Or visit this web page: http://lists.utsouthwestern.edu/mailman/confirm/histonet/0e4978ec2aad5bf6fb354d81c2c8deb859bb9d96 Or include the following line -- and only the following line -- in a message to histonet-request@lists.utsouthwestern.edu: confirm 0e4978ec2aad5bf6fb354d81c2c8deb859bb9d96 Note that simply sending a `reply' to this message should work from most mail readers, since that usually leaves the Subject: line in the right form (additional "Re:" text in the Subject: is okay). If you do not wish to be subscribed to this list, please simply disregard this message. If you think you are being maliciously subscribed to the list, or have any other questions, send them to histonet-owner@lists.utsouthwe From cooperms <@t> mail.nih.gov Thu Oct 9 20:38:46 2008 From: cooperms <@t> mail.nih.gov (Cooperman, Sharon (NIH/NICHD) [E]) Date: Thu Oct 9 20:38:57 2008 Subject: [Histonet] having problems with histonet Message-ID: <2B184EA4ADDB0D41845D8B3730B10F651DA981@NIHCESMLBX7.nih.gov> Hi, I'm having problems with the histonet. It doesn't recognize me as a subscriber and when I tried to subscribe with a new email address I got the following message on replying to the confirmation email: X-IronPortListener: CES-Inbound X-SBRS: 3.3 X-IronPort-Anti-Spam-Filtered: true X-IronPort-Anti-Spam-Result: AgYBALtM7kjHpZiihWdsb2JhbAALkkmBBQEBAQoHBAoHEQWJN5pHhh08gWo Subject: The results of your email commands From: histonet-bounces@lists.utsouthwestern.edu To: cooperms@mail.nih.gov Date: Thu, 09 Oct 2008 20:30:30 -0500 X-BeenThere: histonet@lists.utsouthwestern.edu List-Id: For the exchange of information pertaining to histotechnology and related fields Sender: histonet-bounces@lists.utsouthwestern.edu X-Scan-Signature: 2afe1b2206e4d1abd6c168b829df7099 X-SA-Exim-Connect-IP: 127.0.0.1 X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to false The results of your email command are provided below. Attached is your original message. - Results: Ignoring non-text/plain MIME parts Invalid confirmation string. Note that confirmation strings expire approximately 3 days after the initial subscription request. If your confirmation has expired, please try to re-submit your original request or message. - Unprocessed: -----Original Message----- From: histonet-request@lists.utsouthwestern.edu = [mailto:histonet-request@lists.utsouthwestern.edu] Sent: Thu 10/9/2008 9:26 PM To: Cooperman, Sharon (NIH/NICHD) [E] Subject: confirm 0e4978ec2aad5bf6fb354d81c2c8deb859bb9d96 =20 Mailing list subscription confirmation notice for mailing list Histonet We have received a request from 128.231.88.4 for subscription of your email address, "cooperms@mail.nih.gov", to the histonet@lists.utsouthwestern.edu mailing list. To confirm that you want to be added to this mailing list, simply reply to this message, keeping the Subject: header intact. 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Received: from [199.242.236.161] (helo=swlx161.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1Ko6pq-00077x-3z for histonet-request@lists.utsouthwestern.edu; Thu, 09 Oct 2008 20:30:30 -0500 Received: from nihxway2out.hub.nih.gov ([128.231.90.110]) by swlx161.swmed.edu with esmtp (Exim 4.69) (envelope-from ) id 1Ko6po-0000V2-RD for histonet-request@lists.utsouthwestern.edu; Thu, 09 Oct 2008 20:30:30 -0500 X-IronPortListener: Outbound_SMTP Received: from nihcessmtp.hub.nih.gov ([128.231.90.115]) by nihxway2out.hub.nih.gov with ESMTP; 09 Oct 2008 21:30:28 -0400 Received: from NIHCESMLBX7.nih.gov ([156.40.71.207]) by NIHCESSMTP.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.3959); Thu, 9 Oct 2008 21:30:27 -0400 X-MimeOLE: Produced By Microsoft Exchange V6.5 Content-class: urn:content-classes:message MIME-Version: 1.0 Content-Type: multipart/mixed; boundary="----_=_NextPart_001_01C92A77.C66CED12" Date: Thu, 9 Oct 2008 21:30:22 -0400 Message-ID: <2B184EA4ADDB0D41845D8B3730B10F651DA97C@NIHCESMLBX7.nih.gov> X-MS-Has-Attach: X-MS-TNEF-Correlator: <2B184EA4ADDB0D41845D8B3730B10F651DA97C@NIHCESMLBX7.nih.gov> Thread-Topic: confirm 0e4978ec2aad5bf6fb354d81c2c8deb859bb9d96 Thread-Index: AckqdzDS5/6borvCR4m02ZfSqaM2DgAAJKa7 References: From: "Cooperman, Sharon (NIH/NICHD) [E]" To: X-OriginalArrivalTime: 10 Oct 2008 01:30:27.0754 (UTC) FILETIME=[C68F88A0:01C92A77] X-Scan-Signature: 30c46b6f917c73ddc12a27b699747596 X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on swlx161.swmed.edu X-Spam-Level: X-Spam-Status: No, score=-4.0 required=5.0 tests=RCVD_IN_DNSWL_MED,SPF_PASS autolearn=disabled version=3.2.5 X-Spam-Relay-Country: US US US Subject: RE: confirm 0e4978ec2aad5bf6fb354d81c2c8deb859bb9d96 X-SA-Exim-Connect-IP: 199.242.236.161 X-SA-Exim-Mail-From: cooperms@mail.nih.gov X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to false -----Original Message----- From: histonet-request@lists.utsouthwestern.edu [mailto:histonet-request@lists.utsouthwestern.edu] Sent: Thu 10/9/2008 9:26 PM To: Cooperman, Sharon (NIH/NICHD) [E] Subject: confirm 0e4978ec2aad5bf6fb354d81c2c8deb859bb9d96 Mailing list subscription confirmation notice for mailing list Histonet We have received a request from 128.231.88.4 for subscription of your email address, "cooperms@mail.nih.gov", to the histonet@lists.utsouthwestern.edu mailing list. To confirm that you want to be added to this mailing list, simply reply to this message, keeping the Subject: header intact. Or visit this web page: http://lists.utsouthwestern.edu/mailman/confirm/histonet/0e4978ec2aad5bf6fb354d81c2c8deb859bb9d96 Or include the following line -- and only the following line -- in a message to histonet-request@lists.utsouthwestern.edu: confirm 0e4978ec2aad5bf6fb354d81c2c8deb859bb9d96 Note that simply sending a `reply' to this message should work from most mail readers, since that usually leaves the Subject: line in the right form (additional "Re:" text in the Subject: is okay). If you do not wish to be subscribed to this list, please simply disregard this message. If you think you are being maliciously subscribed to the list, or have any other questions, send them to histonet-owner@lists.utsouthwe From cooperms <@t> mail.nih.gov Thu Oct 9 20:57:27 2008 From: cooperms <@t> mail.nih.gov (Cooperman, Sharon (NIH/NICHD) [E]) Date: Thu Oct 9 20:57:32 2008 Subject: [Histonet] does anyone know if ferritin works as a marker in flow cytometry? Message-ID: <2B184EA4ADDB0D41845D8B3730B10F651DA982@NIHCESMLBX7.nih.gov> Dear Histonetters, Does anyone have experience or suggestions for using ferritin (anti-ferritin antibody) as a marker in flow cytometry? I know this is a weird question but I thought I'd ask anyway. Thanks, Sharon Also - sorry for posting the previous message about problems with the histonet on the list - I was having problems posting for some reason - I guess the problem resolved. I couldn't think of any other way to get through because my emails to other histonet addresses seemed to bounce. From ccrowder <@t> vetmed.lsu.edu Thu Oct 9 23:02:34 2008 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Thu Oct 9 23:05:17 2008 Subject: [Histonet] section thickness Message-ID: Joyce - Our lab cuts tissues from a variety of sources. We cut most tissues at 3 microns. Since we take a lot of pictures and cells were considered 4 microns thick, 3 microns shows only on thickness and good for pictures. The exceptions are kidneys, cut a 2 microns and CNS tissues cut at 5-6 microns. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From louise.renton <@t> gmail.com Fri Oct 10 02:30:11 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Oct 10 02:30:18 2008 Subject: [Histonet] Tol Blue recipe Message-ID: hello all, could someone please share with me the recipe/protocol for Toluidine blue stain (Sterchi and Eurell)? I want to try a combination with Macneal"s tetrachrome Much appreciated. have a great weekend -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From AWeiss <@t> shorememorial.org Fri Oct 10 06:45:54 2008 From: AWeiss <@t> shorememorial.org (AWeiss@shorememorial.org) Date: Fri Oct 10 06:46:52 2008 Subject: [Histonet] Re: Histonet Digest, Vol 59, Issue 10 In-Reply-To: <20081009170128.46F37799E42@smhex1.shorememorial.org> Message-ID: I am a cytotechnologist and for making cell blocks I use 25.occ specimen in a 50.0cc conical tube add 95% alcohol to bring to 45.0cc. You will notice a precipitate will form. Mix well. Centrifuge for 10 mins and then let sit for at least 1 hr. You will find there is a hard button at the bottom. That will be your cell block. If you have a small amt of sample or you have small pieces of tissue from a FNA you can purchase Histogel, this acts like an agar type substance. I melt Histogel tube in microwave 10 seconds usually does it and then add this small amount just enough to cover specimen. Let this sit for a while till it gels. If you need it to gel rapidly just put it into the refrigerator or freezer for a short time and a button will be formed. Place that in a cassette and process as cell block Hope this helps Hello fellows. I am Cyto/ Histology Sup. If there is any one with Non-gyns or GN procedure on cell block preparation or methodology please share with me your ideas or procedure. I need also feedback on Thrombin Clot method. Thanks Jaime Plata MT HTL (ASCP) enrriq88@yahoo.com Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org From njoydobro <@t> aol.com Fri Oct 10 08:02:17 2008 From: njoydobro <@t> aol.com (njoydobro@aol.com) Date: Fri Oct 10 08:02:36 2008 Subject: [Histonet] Pepsin Message-ID: <8CAF8EF8EEFC044-A94-342@WEBMAIL-MB07.sysops.aol.com> Does anyone have a catalogue # for the Pepsin used in Brigati's stable pepsin? Thanks, Gene Cleveland Clinic From Dorothy.L.Webb <@t> HealthPartners.Com Fri Oct 10 09:20:59 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Oct 10 09:21:10 2008 Subject: [Histonet] Validation Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635A0C@hpes1.HealthPartners.int> What are most labs using for valdiation of IHC's from equipment to equipment? Do you use your own tissue to make TMA's or do you purchase them for equipment validation, antibody to antibody? Does anyone have a good source for ordering TMA's or if you make your own, can you help us out with your process? A whole new area of discipline for us and any help would be greatly appreciated!! Thank you ahead of time!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From themagoos <@t> rushmore.com Fri Oct 10 09:21:49 2008 From: themagoos <@t> rushmore.com (Jason and Heather) Date: Fri Oct 10 09:21:55 2008 Subject: [Histonet] IHC antibody Expiration question for CAP Message-ID: <1270.1223648509@rushmore.com> CAP question ANP.22432 states: Are all immunohistochemical reagents used within their indicated expiration dates? I am wondering what labs across the country are doing to answer this question. IHC antibodies are very expensive and can be validated on every run that is made. Some of these concentrated antibodies are diluted out so far that only a very small portion of them are used before they expire. Are some labs still using outdated antibodies? If so how do you get away with it when you are inspected by CAP? Or are you paying the high prices to throw out the majority of your antibodies so you can answer this question yes? I thank you in advance to your response. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com From LSebree <@t> uwhealth.org Fri Oct 10 09:27:28 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Fri Oct 10 09:27:38 2008 Subject: [Histonet] IHC antibody Expiration question for CAP In-Reply-To: <1270.1223648509@rushmore.com> Message-ID: We do not use expired antibodies. For those that have a small volume of requests or are diluted way out there, we buy concentrates in the smallest volumes we can find. Manufactures like NeoMarkers sell 100ul sizes. By carefully tracking usage and dilution factors, we rarely have an issue with expiring antibodies. Those few we do have that expire, we send to Patsy Ruegg for her bank. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason and Heather Sent: Friday, October 10, 2008 9:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC antibody Expiration question for CAP CAP question ANP.22432 states: Are all immunohistochemical reagents used within their indicated expiration dates? I am wondering what labs across the country are doing to answer this question. IHC antibodies are very expensive and can be validated on every run that is made. Some of these concentrated antibodies are diluted out so far that only a very small portion of them are used before they expire. Are some labs still using outdated antibodies? If so how do you get away with it when you are inspected by CAP? Or are you paying the high prices to throw out the majority of your antibodies so you can answer this question yes? I thank you in advance to your response. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Fri Oct 10 09:31:44 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Oct 10 09:31:53 2008 Subject: [Histonet] IHC antibody Expiration question for CAP In-Reply-To: <1270.1223648509@rushmore.com> Message-ID: We have determined the number of times an antibody is requested/year, and what the average shelf-life is that a particular vendor supplies. If we determine that the antibody will not be used by that time, we'll check to see if a smaller amount can be purchased. If not, we'll discuss the options with the vendor. That may be returning the unused portion and getting a credit, or changing to another vendor that may have longer expirations. We do not keep the antibodies beyond their expiration. If it is a test ordered infrequently, we may also send it out for the TC, and have the PC done in-house. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jason and Heather Sent: Friday, October 10, 2008 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC antibody Expiration question for CAP CAP question ANP.22432 states: Are all immunohistochemical reagents used within their indicated expiration dates? I am wondering what labs across the country are doing to answer this question. IHC antibodies are very expensive and can be validated on every run that is made. Some of these concentrated antibodies are diluted out so far that only a very small portion of them are used before they expire. Are some labs still using outdated antibodies? If so how do you get away with it when you are inspected by CAP? Or are you paying the high prices to throw out the majority of your antibodies so you can answer this question yes? I thank you in advance to your response. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From japoteete <@t> saintfrancis.com Fri Oct 10 09:32:14 2008 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Fri Oct 10 09:32:27 2008 Subject: [Histonet] IHC antibody Expiration question for CAP In-Reply-To: <1270.1223648509@rushmore.com> Message-ID: We are not permitted to use anything that is expired, no matter what it is. Yes, the lab takes a major hit because of this regulation. I send my expired antibodies to Patsy Ruegg so she can use them for experimental purposes. It isn't a total loss, just a very expensive one. Thanks for the reminder, because I need to send her another bunch. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Lab Saint Francis Hospital Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason and Heather Sent: Friday, October 10, 2008 9:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC antibody Expiration question for CAP CAP question ANP.22432 states: Are all immunohistochemical reagents used within their indicated expiration dates? I am wondering what labs across the country are doing to answer this question. IHC antibodies are very expensive and can be validated on every run that is made. Some of these concentrated antibodies are diluted out so far that only a very small portion of them are used before they expire. Are some labs still using outdated antibodies? If so how do you get away with it when you are inspected by CAP? Or are you paying the high prices to throw out the majority of your antibodies so you can answer this question yes? I thank you in advance to your response. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arsenn <@t> hsh.org Fri Oct 10 09:41:07 2008 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Fri Oct 10 09:41:19 2008 Subject: [Histonet] Breast Inking Message-ID: Hello Histonetters, We've received a request from our breast doc to see if we can find a sterile ink-inside and out. It doesn't necessarily have to be India Ink-our PA can re-ink the breast specimen when it comes to the histo department, but the doc is looking for something other than the bottles she's currently using because she wastes so much on each patient. We suggested the little vials/bottles of ink we found in several catalogues, but the doc says that these are not externally sterile. Can anyone let me know what they use in the OR, or if you have any suggestions, please let me know! Thank you in advance for all your help....as always!!! Have a great weekend everyone! Happy FRIDAY! Amy Senn Camp Hill PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From ploykasek <@t> phenopath.com Fri Oct 10 09:43:55 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Oct 10 09:44:15 2008 Subject: [Histonet] IHC antibody Expiration question for CAP In-Reply-To: <1270.1223648509@rushmore.com> Message-ID: You can only use unexpired reagents on patient material. We donate useful expired (but still working) antibodies to a local histology school. Sending the reagents to Patsy Reugg is another excellent option. Patti Loykasek BS, HTL, QIHC Clinical IHC Supervisor PhenoPath Laboratories Seattle, WA > CAP question ANP.22432 states: Are all immunohistochemical reagents used > within their indicated expiration dates? > > I am wondering what labs across the country are doing to answer this question. > IHC antibodies are very expensive and can be validated on every run that is > made. Some of these concentrated antibodies are diluted out so far that only a > very small portion of them are used before they expire. Are some labs still > using outdated antibodies? If so how do you get away with it when you are > inspected by CAP? Or are you paying the high prices to throw out the majority > of your antibodies so you can answer this question yes? > > I thank you in advance to your response. > > Jason McGough HT(ASCP) > Account Representative - Anatomic Pathology > Clinical Laboratory of the Black Hills > 2805 5th Street Suite 210 > Rapid City, SD 57701 > 605-343-2267 Ext 127 > 605-718-3779 (Fax) > jmcgough@clinlab.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From kimtournear <@t> yahoo.com Fri Oct 10 09:51:10 2008 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Fri Oct 10 09:51:13 2008 Subject: [Histonet] IHC antibody Expiration question for CAP In-Reply-To: Message-ID: <610350.48596.qm@web54207.mail.re2.yahoo.com> If it's something we're only going to use less thata dozen?times a year, we sent them to an outside source like US labs, Oncotech, etc.? I can't justify the cost or the tech time to work it up on something rarely used. ~Kim ~ ? ~Don't?let your life end before it begins~ ? OU Rocks!!!! --- On Fri, 10/10/08, Poteete, Jacquie A. wrote: From: Poteete, Jacquie A. Subject: RE: [Histonet] IHC antibody Expiration question for CAP To: themagoos@rushmore.com, histonet@lists.utsouthwestern.edu Date: Friday, October 10, 2008, 7:32 AM We are not permitted to use anything that is expired, no matter what it is. Yes, the lab takes a major hit because of this regulation. I send my expired antibodies to Patsy Ruegg so she can use them for experimental purposes. It isn't a total loss, just a very expensive one. Thanks for the reminder, because I need to send her another bunch. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Lab Saint Francis Hospital Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason and Heather Sent: Friday, October 10, 2008 9:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC antibody Expiration question for CAP CAP question ANP.22432 states: Are all immunohistochemical reagents used within their indicated expiration dates? I am wondering what labs across the country are doing to answer this question. IHC antibodies are very expensive and can be validated on every run that is made. Some of these concentrated antibodies are diluted out so far that only a very small portion of them are used before they expire. Are some labs still using outdated antibodies? If so how do you get away with it when you are inspected by CAP? Or are you paying the high prices to throw out the majority of your antibodies so you can answer this question yes? I thank you in advance to your response. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Fri Oct 10 09:54:12 2008 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Fri Oct 10 09:54:18 2008 Subject: [Histonet] Any experience with the Mohs "cryo embedder" In-Reply-To: <5b6eb13e0810091245u20a311cbld148b1e319411d@mail.gmail.com> Message-ID: <548832.31388.qm@web54206.mail.re2.yahoo.com> I've done both of those methods. I used the cyro embedder at a mohs lab here for 2 yrs. It's the next best thing to sliced bread and is great for getting those epi's down. I will get the vendor info and cat # and post it on the net today. The last one I bought cost appx $1600.00 but was well worth it when I had to start training new tech from within the office. ~Kim ~ ? ~Don't?let your life end before it begins~ ? OU Rocks!!!! --- On Thu, 10/9/08, Mark Tarango wrote: From: Mark Tarango Subject: Re: [Histonet] Any experience with the Mohs "cryo embedder" To: "Ingles Claire" Cc: histonet@lists.utsouthwestern.edu Date: Thursday, October 9, 2008, 12:45 PM Does anyone else freeze the tissue to a glass slide to get it embedded flat? That's how I've been show and it seems to work well. I've used plastic embedding molds too, but they don't always give me a flat surface. Mark On Thu, Oct 9, 2008 at 12:02 PM, Ingles Claire wrote: > Sandra: > Who is selling this, and how does it work? I have been testing one type of > embedder, but I know there are several out there. > Claire > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Esparza, > Sandra > Sent: Thu 10/9/2008 11:20 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Any experience with the Mohs "cryo embedder" > > > > Hi Everyone, > > > > I am new to the Mohs technique for dermatology specimens and am trying > to find out if anyone has used the Cryo Embedder system. It sound great > but I don't want to order it until I'm sure that it is user friendly. > Any help with the Mohs technique would be greatly appreciated. Thanks, > > > Sandra HT (ASCP) > > Dell Children's Hospital > > Austin, Texas > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Fri Oct 10 09:55:15 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Oct 10 09:55:41 2008 Subject: [Histonet] Secondary antibody binding non-specifically Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C66@LSRIEXCH1.lsmaster.lifespan.org> Has anyone run into problems with a secondary antibody binding non-specifically to the tissue? I am using a mouse monoclonal primary with a standard Vector Elite anti-mouse secondary, following antigen unmasking, followed by ABC and DAB as usual. The tissues are human tumors. A couple of them are showing strong staining in many areas where there should be no staining. Omitting the primary antibody makes no difference. Omitting both antibodies and using just the ABC complex results in a clean slide, so endogenous biotin is not the problem. Apparently the secondary antibody is binding non-specifically to the tumor. Has anyone else run into such a problem? How did you address it? From Terry.Marshall <@t> rothgen.nhs.uk Fri Oct 10 10:06:50 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Oct 10 10:06:58 2008 Subject: [Histonet] IHC antibody Expiration question for CAP References: Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F561@TRFT-EX01.xRothGen.nhs.uk> What a ridiculous rule. If the antibody works on a control, THAT is the test for whether it is useful or not, not an arbitrary date printed on the tube. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: 10 October 2008 15:32 To: themagoos@rushmore.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC antibody Expiration question for CAP We are not permitted to use anything that is expired, no matter what it is. Yes, the lab takes a major hit because of this regulation. I send my expired antibodies to Patsy Ruegg so she can use them for experimental purposes. It isn't a total loss, just a very expensive one. Thanks for the reminder, because I need to send her another bunch. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Lab Saint Francis Hospital Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason and Heather Sent: Friday, October 10, 2008 9:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC antibody Expiration question for CAP CAP question ANP.22432 states: Are all immunohistochemical reagents used within their indicated expiration dates? I am wondering what labs across the country are doing to answer this question. IHC antibodies are very expensive and can be validated on every run that is made. Some of these concentrated antibodies are diluted out so far that only a very small portion of them are used before they expire. Are some labs still using outdated antibodies? If so how do you get away with it when you are inspected by CAP? Or are you paying the high prices to throw out the majority of your antibodies so you can answer this question yes? I thank you in advance to your response. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Fri Oct 10 10:07:55 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Oct 10 10:08:02 2008 Subject: [Histonet] Expired Antibodies Message-ID: <879264.89295.qm@web1104.biz.mail.sk1.yahoo.com> I too am a research contract lab and would gladly accept donations of expired antibodies. Paula Pierce, BS, HT(ASCP)HTL Excalibur Pathology, Inc. 631 N Broadway Ave. Moore, OK 73160 405-759-3953 www.excaliburpathology.com From japoteete <@t> saintfrancis.com Fri Oct 10 10:17:31 2008 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Fri Oct 10 10:17:40 2008 Subject: [Histonet] IHC antibody Expiration question for CAP In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F561@TRFT-EX01.xRothGen.nhs.uk> Message-ID: I agree, it is a very ridiculous rule, and I don't like having to eliminate perfectly good antibodies or reagents. When you look at the CAP guidelines, there are lots of ridiculous rules, in my humble opinion. Let the flaming begin! Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Lab Saint Francis Hospital Tulsa, OK -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Friday, October 10, 2008 10:07 AM To: Poteete, Jacquie A.; themagoos@rushmore.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC antibody Expiration question for CAP What a ridiculous rule. If the antibody works on a control, THAT is the test for whether it is useful or not, not an arbitrary date printed on the tube. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: 10 October 2008 15:32 To: themagoos@rushmore.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC antibody Expiration question for CAP We are not permitted to use anything that is expired, no matter what it is. Yes, the lab takes a major hit because of this regulation. I send my expired antibodies to Patsy Ruegg so she can use them for experimental purposes. It isn't a total loss, just a very expensive one. Thanks for the reminder, because I need to send her another bunch. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Lab Saint Francis Hospital Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason and Heather Sent: Friday, October 10, 2008 9:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC antibody Expiration question for CAP CAP question ANP.22432 states: Are all immunohistochemical reagents used within their indicated expiration dates? I am wondering what labs across the country are doing to answer this question. IHC antibodies are very expensive and can be validated on every run that is made. Some of these concentrated antibodies are diluted out so far that only a very small portion of them are used before they expire. Are some labs still using outdated antibodies? If so how do you get away with it when you are inspected by CAP? Or are you paying the high prices to throw out the majority of your antibodies so you can answer this question yes? I thank you in advance to your response. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Fri Oct 10 10:18:49 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Oct 10 10:19:01 2008 Subject: [Histonet] RELIA Histology Jobs Update A Quick post before the weekend! Message-ID: Hi Histonetters! I just wanted to do a quick post before we all get busy with our weekend football games, festivals and all the other great things that we get to do this time of year when the weather is great everywhere!! I have some new positions that I am working on that I wanted to tell you about. I have new management positions in Austin, TX and Manchester, NH and I have new technician/technologist positions in San Francisco Bay, Kansas City, North of Boston, Seattle, Spokane and Coastal North Carolina. All of these positions are full time permanent positions with premier companies who offer excellent compensaton, benefits and relocation assistance. If you or anyone you know is interested in any of these positions please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. Remember I offer over 25 years of general recruiting experience combined with 5 years dedicated exclusively to the nationwide permanent placement of histology professionals. I only work with the best clients in the industry and am proud to say that my candidates are my first priority. I want you to be happy in your career. So before you check with any of the "johnny come lately" Healthcare Recruiters Call Me First! Between your histology skills and my recruiting expertise we can make that next great career move happen for you. Have A Great Weekend!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia From LSebree <@t> uwhealth.org Fri Oct 10 10:21:14 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Fri Oct 10 10:21:19 2008 Subject: [Histonet] IHC antibody Expiration question for CAP In-Reply-To: Message-ID: I don't think you'll get much flaming on that statement Jacquie, just a lot of agreement! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: Friday, October 10, 2008 10:18 AM To: Marshall Terry Dr,Consultant Histopathologist; themagoos@rushmore.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC antibody Expiration question for CAP I agree, it is a very ridiculous rule, and I don't like having to eliminate perfectly good antibodies or reagents. When you look at the CAP guidelines, there are lots of ridiculous rules, in my humble opinion. Let the flaming begin! Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Lab Saint Francis Hospital Tulsa, OK -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Friday, October 10, 2008 10:07 AM To: Poteete, Jacquie A.; themagoos@rushmore.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC antibody Expiration question for CAP What a ridiculous rule. If the antibody works on a control, THAT is the test for whether it is useful or not, not an arbitrary date printed on the tube. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: 10 October 2008 15:32 To: themagoos@rushmore.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC antibody Expiration question for CAP We are not permitted to use anything that is expired, no matter what it is. Yes, the lab takes a major hit because of this regulation. I send my expired antibodies to Patsy Ruegg so she can use them for experimental purposes. It isn't a total loss, just a very expensive one. Thanks for the reminder, because I need to send her another bunch. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Lab Saint Francis Hospital Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason and Heather Sent: Friday, October 10, 2008 9:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC antibody Expiration question for CAP CAP question ANP.22432 states: Are all immunohistochemical reagents used within their indicated expiration dates? I am wondering what labs across the country are doing to answer this question. IHC antibodies are very expensive and can be validated on every run that is made. Some of these concentrated antibodies are diluted out so far that only a very small portion of them are used before they expire. Are some labs still using outdated antibodies? If so how do you get away with it when you are inspected by CAP? Or are you paying the high prices to throw out the majority of your antibodies so you can answer this question yes? I thank you in advance to your response. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lyonm <@t> upstate.edu Fri Oct 10 10:31:02 2008 From: lyonm <@t> upstate.edu (Michael J. Lyon, Ph.D.) Date: Fri Oct 10 10:31:17 2008 Subject: [Histonet] UMFIX Message-ID: <6D113B715CBB4D29B5A59B30F0AA0709@LyonOffice> Does anyone have experience with UMFIX a methanol/polyethylene glycol fixative sold by Sakura Tissue-Tek, Torrance, CA? If so good, bad or indifferent? Thanks Michael J. Lyon, Ph.D. Otolaryngology Research Lab SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 Voice 315-464-7253 Fax 315-464-5572 From f_ed <@t> hotmail.com Fri Oct 10 10:38:42 2008 From: f_ed <@t> hotmail.com (ed flores) Date: Fri Oct 10 10:38:46 2008 Subject: [Histonet] (no subject) Message-ID: PLEASE TAKE ME OFF THE MAILING LIST FOR THE TIME BEING FOR I WILL BE OUT OF TOWN FOR NEXT COUPLE OF MONTHS. MY ADDRESS IS F_ED@HOTMAIL.COM. THANKS, ED. _________________________________________________________________ Stay up to date on your PC, the Web, and your mobile phone with Windows Live. http://clk.atdmt.com/MRT/go/msnnkwxp1020093185mrt/direct/01/ From kimtournear <@t> yahoo.com Fri Oct 10 10:41:00 2008 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Fri Oct 10 10:41:05 2008 Subject: [Histonet] Any experience with the Mohs "cryo embedder" Message-ID: <998009.96140.qm@web54201.mail.re2.yahoo.com> OK, Here's the Cryo Embedder info: ? The Vendor is: Belaire Instruments 1-973-912-8900 ? Contact is: Sheila Skolnick ext 112 ? Price is: $1695.00 When ordering, there is no cat #. It's ordered as a "Complete Cryo-Embedder Package" ? I've been doing Mohs for years and have used all of the old methods and used the cryo?embedder at my last job for 2yrs. It's the greatest thing since sliced bread.? If you want to make sure those epi's are down, then this is the way to go....It's very easy to use and a great tool when training new techs. ? Good luck and I hope I've been some help. ? Kim Tournear, HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center? Tucson, AZ ~Don't?let your life end before it begins~ ? OU Rocks!!!! --- On Fri, 10/10/08, Kim Tournear wrote: From: Kim Tournear Subject: Re: [Histonet] Any experience with the Mohs "cryo embedder" To: "Histonet" Date: Friday, October 10, 2008, 7:54 AM I've done both of those methods. I used the cyro embedder at a mohs lab here for 2 yrs. It's the next best thing to sliced bread and is great for getting those epi's down. I will get the vendor info and cat # and post it on the net today. The last one I bought cost appx $1600.00 but was well worth it when I had to start training new tech from within the office. ~Kim ~ ? ~Don't?let your life end before it begins~ ? OU Rocks!!!! --- On Thu, 10/9/08, Mark Tarango wrote: From: Mark Tarango Subject: Re: [Histonet] Any experience with the Mohs "cryo embedder" To: "Ingles Claire" Cc: histonet@lists.utsouthwestern.edu Date: Thursday, October 9, 2008, 12:45 PM Does anyone else freeze the tissue to a glass slide to get it embedded flat? That's how I've been show and it seems to work well. I've used plastic embedding molds too, but they don't always give me a flat surface. Mark On Thu, Oct 9, 2008 at 12:02 PM, Ingles Claire wrote: > Sandra: > Who is selling this, and how does it work? I have been testing one type of > embedder, but I know there are several out there. > Claire > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Esparza, > Sandra > Sent: Thu 10/9/2008 11:20 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Any experience with the Mohs "cryo embedder" > > > > Hi Everyone, > > > > I am new to the Mohs technique for dermatology specimens and am trying > to find out if anyone has used the Cryo Embedder system. It sound great > but I don't want to order it until I'm sure that it is user friendly. > Any help with the Mohs technique would be greatly appreciated. Thanks, > > > Sandra HT (ASCP) > > Dell Children's Hospital > > Austin, Texas > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Fri Oct 10 11:06:28 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Oct 10 11:06:39 2008 Subject: [Histonet] Any experience with the Mohs "cryo embedder" In-Reply-To: References: <3D79F47DC92B204F9E5D35C885DFC5CB01003C01@AUSEX2VS1.seton.org> <08A0A863637F1349BBFD83A96B27A50A12017B@uwhis-xchng3.uwhis.hosp.wisc.edu> <5b6eb13e0810091245u20a311cbld148b1e319411d@mail.gmail.com> Message-ID: <5b6eb13e0810100906h25433b86k5f7670e5e9e43322@mail.gmail.com> The Leica CM 1100 doesn't let you adjust the angle of the block face. You have to be flat. I'll admit it's a major drawback. I didn't even realize it until you mentioned this. I just got off the phone with leica and they don't have anything to replace the specimen holder. Looks like I'm just stuck that way. Mark On Thu, Oct 9, 2008 at 2:49 PM, Mickie Johnson wrote: > Hi Mark, > > Thanks for your input on how the Leica CM 1100 is working for you. > Regarding > the best way to embed tissues for Mohs. Yes, the slide method is the most > accurate to get the tissue flat as long as the epidermis is relaxed so it > lays down on the glass too. Watch out for trapped air bubbles under the > specimen. I smear a thin coat of O.C.T. on the slide and this helps hold > the > epi down during embedding. Does the Leica 1100 allow you to reposition the > block face parallel to the knife when you embed? That is important so that > you don't cut any more tissue away than necessary to get a full section of > epidermis and deep margin. There is also a device which is very helpful in > embedding Mohs specimens to minimize the need to line up the block face to > the knife. Many labs use it. I carry one where ever I go to help me embed > fast and accurately. If you would like to know more give me a call. Good > Luck! > > Best Regards, > > Mickie > > Mickie Johnson, B.S., HTL(ASCP) > Mohs Histology Consulting Services, LLC > & Mohs Lab Staffing > 2507 S. Manito Blvd. > Spokane, WA 99203 > 509-954-7134 > FAX 509-624-3926 > Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com > Email: mickie25@netzero.net > > DISCLAIMER: > This message is intended for the sole use of the addressee, and may contain > information that is privileged, confidential and exempt from disclosure > under applicable law. If you are not the addressee you are hereby notified > that you may not use, copy, disclose, or distribute to anyone the message > or > any information contained in the message. If you have received this message > in error, please immediately advise the sender by reply email and delete > this message. > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark > Tarango > Sent: Thursday, October 09, 2008 12:45 PM > To: Ingles Claire > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Any experience with the Mohs "cryo embedder" > > Does anyone else freeze the tissue to a glass slide to get it embedded > flat? That's how I've been show and it seems to work well. > > I've used plastic embedding molds too, but they don't always give me a flat > surface. > Mark > > On Thu, Oct 9, 2008 at 12:02 PM, Ingles Claire > wrote: > > > Sandra: > > Who is selling this, and how does it work? I have been testing one type > of > > embedder, but I know there are several out there. > > Claire > > > > ________________________________ > > > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Esparza, > > Sandra > > Sent: Thu 10/9/2008 11:20 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Any experience with the Mohs "cryo embedder" > > > > > > > > Hi Everyone, > > > > > > > > I am new to the Mohs technique for dermatology specimens and am trying > > to find out if anyone has used the Cryo Embedder system. It sound great > > but I don't want to order it until I'm sure that it is user friendly. > > Any help with the Mohs technique would be greatly appreciated. Thanks, > > > > > > Sandra HT (ASCP) > > > > Dell Children's Hospital > > > > Austin, Texas > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG - http://www.avg.com > Version: 8.0.173 / Virus Database: 270.7.6/1714 - Release Date: 10/8/2008 > 7:01 AM > > From asmith <@t> mail.barry.edu Fri Oct 10 11:14:10 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Oct 10 11:16:38 2008 Subject: [Histonet] Toluene vs. Xylene In-Reply-To: <571831.37907.qm@web65709.mail.ac4.yahoo.com> References: <527229.12110.qm@web45910.mail.sp1.yahoo.com> <571831.37907.qm@web65709.mail.ac4.yahoo.com> Message-ID: Although both are flammable and give off toxic fumes, toluene is slightly more dangerous because it has a higher vapor pressure. The flash point of toluene is 5 degrees C; the flash point of xylene is around 25 degrees C (Merck index; Lewis & Sax say 38 degrees C). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, October 09, 2008 10:38 AM To: histonet@lists.utsouthwestern.edu; James Dooley Subject: Re: [Histonet] Toluene vs. Xylene Not really and both are as dangerous and both should be avoided. As a matter of fact xylene began to be used in the mid 1950s to substitute chemicals like toluene, until it was found that both were equally dangerous. Toluene is present in many mounting media. Ren? J. --- On Thu, 10/9/08, James Dooley wrote: From: James Dooley Subject: [Histonet] Toluene vs. Xylene To: histonet@lists.utsouthwestern.edu Date: Thursday, October 9, 2008, 10:16 AM Is there a significant difference between using xylene and toluene when deparaffinizing embedding tissue? Thank you, James _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kerry.l.crabb <@t> gsk.com Fri Oct 10 11:17:16 2008 From: kerry.l.crabb <@t> gsk.com (kerry.l.crabb@gsk.com) Date: Fri Oct 10 11:17:37 2008 Subject: [Histonet] Kerry L Crabb/PharmRD/GSK is out of the office. Message-ID: I will be out of the office starting 10-Oct-2008 and will not return until 14-Oct-2008. During my absence contact Jim Nold about your anatomic path issues. I will respond to messages when I return. From amartinez <@t> carisdx.com Fri Oct 10 11:22:00 2008 From: amartinez <@t> carisdx.com (Martinez, Angela) Date: Fri Oct 10 11:22:05 2008 Subject: [Histonet] Great opportunity for Histotechnician Message-ID: <9B8A3AC772C7F64680392A7CB8FBFB0F06146277@s-irv-ex301.PathologyPartners.intranet> Great opportunity for Histotechnician in brand new laboratory! Gastrointestinal Associates of North Texas in Ft Worth , is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and meet CLIA-88 regulations to perform gross dissection. Prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. The position offers competitive salary, medical insurance, retirement plan, and vacation /sick leave. Interested applicants should e-mail resumes to Meredith Hale at mhale@carisdx.com . Meredith Hale HT (ASCP) Technical Director Caris Diagnostics 8400 Esters Blvd. Ste. 190 Irving, Texas 75063 469-648-8253 Cell 214-596-2219 Office 972-929-9996 Fax Angela Martinez Internal Recruiter Caris Diagnostics 8400 Esters Blvd. Suite 190 Irving, Texas 75063 (214) 277-8700 Phone (214) 596-7490Fax From sharon.osborn <@t> comcast.net Fri Oct 10 11:49:34 2008 From: sharon.osborn <@t> comcast.net (sharon.osborn@comcast.net) Date: Fri Oct 10 11:49:39 2008 Subject: [Histonet] GO SOONERS! Message-ID: <101020081649.26860.48EF879E00011BF3000068EC2216525806029D010D9C01D202019D0E089C@comcast.net> Histonetters! Taking off on Kim Tournear's message of "OU Rocks!" to say one of the best college games of the year if not so far this century will be played out in the Cotton Bowl, Dallas tomorrow morning at 11:00AM CDT. It is between Oklahom #1 and Univ Texas #5 and will be a true grudge match!. So, GO SOONERS, Hook 'em Horns Those who are not football fans may enjoy following a WWII Liberty Ship, SS Jeremiah O'Brien as she sails the San Francisco Bay this Sat-Sun for Fleet week. You can track her at http://hd-sf.com/livemap.html I am a volunteer crew member so will root for my team while sailing! Oh, by the way, it is our summer now. Happy weekend! Sharon Osborn ThermoFisher Fremont, CA From boor <@t> email.cz Fri Oct 10 12:24:14 2008 From: boor <@t> email.cz (=?us-ascii?Q?Peter=20Boor?=) Date: Fri Oct 10 12:24:23 2008 Subject: [Histonet] (no subject) Message-ID: <355.824-16238-259272359-1223659454@email.cz> Hi everybody, does anyone know a reliable marker for kidney glomerular and tubulointerstitial endothelial cells in rats and mice? Which antibodies do work?? In our hands only JG12 Ab works for rat glomerular but not interstitial endothelium and VEGFR2 in mice the other way round. Many thanks in advance, Peter Boor From burch007 <@t> mc.duke.edu Fri Oct 10 12:39:00 2008 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Fri Oct 10 12:39:10 2008 Subject: [Histonet] Pepsin In-Reply-To: <8CAF8EF8EEFC044-A94-342@WEBMAIL-MB07.sysops.aol.com> Message-ID: Gene: Dako and Biocare both sell the stable pepsin solution. It is easy to prepare if you want to make it yourself. I use this pepsin everyday. Here is the recipe that Dr. Brigati gave me. Best Regards, Jim Burchette Brigati?s 0.25% Stable Pepsin Solution Reagents Pepsin, #P7012, Sigma Chemical Co. ** 10X Automation Buffer, #BMM30, Fisher Scientific ** Preparation 40 ml 10X Automation Buffer 356 ml distilled water Adjust mixture of Automation Buffer and water to pH 2.0. Add 1.0 gram of P7012 pepsin (it is important to add the pepsin after the pH adjustment). Place container on a stir plate with gentle stirring allowing the pepsin to dissolve. The solution is sometimes cloudy at first, but will become clear after sitting overnight. Store at 4 C. Apply at room temperature Digest formalin-fixed, paraffin embedded tissue sections for 13-15 minutes at 37-40. Wash with IHC procedural buffer or DI water to stop enzyme digestion and to neutralize the pH. ** The Biomeda TBS automation buffer is no longer available. I now use the Automation Wash TBS Buffer from Biocare Medical. It is a 20X concentrate. Adjust the volume of water and buffer accordingly. You could also use 396 ml of 1X TBS. Dr. Brigati passed away a year ago. He was a great pathologist, mentor and friend. njoydobro@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 10/10/2008 09:05 AM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Pepsin Does anyone have a catalogue # for the Pepsin used in Brigati's stable pepsin? Thanks, Gene Cleveland Clinic _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jfish <@t> gladstone.ucsf.edu Fri Oct 10 13:37:51 2008 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Fri Oct 10 13:38:47 2008 Subject: [Histonet] GO SOONERS! In-Reply-To: <101020081649.26860.48EF879E00011BF3000068EC2216525806029D010D9C01D202019D0E089C@comcast.net> References: <101020081649.26860.48EF879E00011BF3000068EC2216525806029D010D9C01D202019D0E089C@comcast.net> Message-ID: <000801c92b07$4d32cc70$4e0d010a@JFISH> Hi Sharon, I watched the Blue Angels practicing yesterday, just outside my lab window here in Mission Bay! I hope they practice again today! They were absolutely beautiful! Have fun sailing tomorrow! Jo Dee ~~Jo Dee Fish~~ Senior Research Technologist The J. David Gladstone Institutes Co-manager Histology and Microscopy Core Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sharon.osborn@comcast.net Sent: Friday, October 10, 2008 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GO SOONERS! Histonetters! Taking off on Kim Tournear's message of "OU Rocks!" to say one of the best college games of the year if not so far this century will be played out in the Cotton Bowl, Dallas tomorrow morning at 11:00AM CDT. It is between Oklahom #1 and Univ Texas #5 and will be a true grudge match!. So, GO SOONERS, Hook 'em Horns Those who are not football fans may enjoy following a WWII Liberty Ship, SS Jeremiah O'Brien as she sails the San Francisco Bay this Sat-Sun for Fleet week. You can track her at http://hd-sf.com/livemap.html I am a volunteer crew member so will root for my team while sailing! Oh, by the way, it is our summer now. Happy weekend! Sharon Osborn ThermoFisher Fremont, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Fri Oct 10 13:47:28 2008 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Oct 10 13:47:32 2008 Subject: [Histonet] Re: Any experience with the Mohs "cryo embedder" (Mark Tarango) Message-ID: <952818.32478.qm@web45105.mail.sp1.yahoo.com> If you would like to try a alternative to the "cryoembedder" you may be interested in ?trying our "Precision cryoembedding system". By embedding tissue face down in freezing temperature "well bars" you adhere tissue perfectly flat to the well floor. Multiple tissue pieces can be precisely prepared in a single flat plane resulting in ?minimal wastage. I have numerous Mohs techniologists using the system and am happy to send a demo set to try for no obligation. Systems can be purchased at a fraction of the price you are describing. Please visit ?pathologyinnovations.com/index.html if you are intersted. Stephen Peters M.D. Vice Chairman?of Pathology Hackensack University Medical Center 201 996 4836 ? Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From rjbuesa <@t> yahoo.com Fri Oct 10 14:16:51 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 10 14:16:55 2008 Subject: [Histonet] UMFIX In-Reply-To: <6D113B715CBB4D29B5A59B30F0AA0709@LyonOffice> Message-ID: <550278.27074.qm@web65713.mail.ac4.yahoo.com> It works as good as methacarn, which is much cheaper. Ren? J. --- On Fri, 10/10/08, Michael J. Lyon, Ph.D. wrote: From: Michael J. Lyon, Ph.D. Subject: [Histonet] UMFIX To: histonet@lists.utsouthwestern.edu Date: Friday, October 10, 2008, 11:31 AM Does anyone have experience with UMFIX a methanol/polyethylene glycol fixative sold by Sakura Tissue-Tek, Torrance, CA? If so good, bad or indifferent? Thanks Michael J. Lyon, Ph.D. Otolaryngology Research Lab SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 Voice 315-464-7253 Fax 315-464-5572 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Fri Oct 10 14:59:04 2008 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Fri Oct 10 14:59:07 2008 Subject: [Histonet] GO SOONERS! In-Reply-To: <101020081649.26860.48EF879E00011BF3000068EC2216525806029D010D9C01D202019D0E089C@comcast.net> Message-ID: <314471.37113.qm@web54203.mail.re2.yahoo.com> I'm from Oklahoma, but live?in Tucson, so I guess that make me an AZ Wildcat now...but a true sooner at heart...AND the two coaches just happen to be brothers, soooo that makes my son in law my rival....LOL...ya'll have a GR8T weekend..... ~Kim ~ ? ~Don't?let your life end before it begins~ ? OU Rocks!!!! --- On Fri, 10/10/08, sharon.osborn@comcast.net wrote: From: sharon.osborn@comcast.net Subject: [Histonet] GO SOONERS! To: histonet@lists.utsouthwestern.edu Date: Friday, October 10, 2008, 9:49 AM Histonetters! Taking off on Kim Tournear's message of "OU Rocks!" to say one of the best college games of the year if not so far this century will be played out in the Cotton Bowl, Dallas tomorrow morning at 11:00AM CDT. It is between Oklahom #1 and Univ Texas #5 and will be a true grudge match!. So, GO SOONERS, Hook 'em Horns Those who are not football fans may enjoy following a WWII Liberty Ship, SS Jeremiah O'Brien as she sails the San Francisco Bay this Sat-Sun for Fleet week. You can track her at http://hd-sf.com/livemap.html I am a volunteer crew member so will root for my team while sailing! Oh, by the way, it is our summer now. Happy weekend! Sharon Osborn ThermoFisher Fremont, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lchen <@t> mednet.ucla.edu Fri Oct 10 15:31:22 2008 From: lchen <@t> mednet.ucla.edu (Leslie Chen) Date: Fri Oct 10 15:31:47 2008 Subject: [Histonet] histology ink washing off Message-ID: <000c01c92b17$354b76b0$3ba62f0a@DHGLABC99E93DF> Hello, I am having a problem with histology tissue marking ink washing off specimens in water, PFA, or 70% alcohol. This is before the tissue even gets processed for paraffin embedding. Does anyone have any suggestions on how to keep the ink from coming off? Thank you. Leslie ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From thisisann <@t> aol.com Fri Oct 10 15:34:24 2008 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Fri Oct 10 15:34:36 2008 Subject: [Histonet] Position in NJ Message-ID: <8CAF92EB78157BF-10E0-17EF@WEBMAIL-MY03.sysops.aol.com> I am looking for a Histology Supervisor in Northern, NJ.? Candidate should have HT/HTL (ASCP) with some supervisory experience.? Qualified candidates should e-mail their resume to Thisisann@aol.com From JWeems <@t> sjha.org Fri Oct 10 15:42:56 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Oct 10 15:43:10 2008 Subject: [Histonet] histology ink washing off In-Reply-To: <000c01c92b17$354b76b0$3ba62f0a@DHGLABC99E93DF> References: <000c01c92b17$354b76b0$3ba62f0a@DHGLABC99E93DF> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA509F0CB@ITSSSXM01V6.one.ads.che.org> Spray with or dip in a solution of 5% acetic acid (vinegar) and the ink will set... Have a good weekend! Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leslie Chen Sent: Friday, October 10, 2008 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histology ink washing off Hello, I am having a problem with histology tissue marking ink washing off specimens in water, PFA, or 70% alcohol. This is before the tissue even gets processed for paraffin embedding. Does anyone have any suggestions on how to keep the ink from coming off? Thank you. Leslie ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From laurie.colbert <@t> huntingtonhospital.com Fri Oct 10 16:11:07 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Oct 10 16:11:11 2008 Subject: [Histonet] histology ink washing off Message-ID: <57BE698966D5C54EAE8612E8941D768303F543E6@EXCHANGE3.huntingtonhospital.com> We spray with a 3% solution of acetic acid. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leslie Chen Sent: Friday, October 10, 2008 1:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histology ink washing off Hello, I am having a problem with histology tissue marking ink washing off specimens in water, PFA, or 70% alcohol. This is before the tissue even gets processed for paraffin embedding. Does anyone have any suggestions on how to keep the ink from coming off? Thank you. Leslie ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sccrshlly <@t> yahoo.com Fri Oct 10 16:34:00 2008 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Fri Oct 10 16:34:06 2008 Subject: [Histonet] Re: Section thickness Message-ID: <18391.71280.qm@web90305.mail.mud.yahoo.com> At the labs I have worked at we cut routine tissue at 3um, lymph nodes and kidney biopsies at 2um, bone marrow clots and biopsies at 1 um. Hope this helps, Shelly From Rcartun <@t> harthosp.org Fri Oct 10 17:09:53 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Oct 10 17:10:09 2008 Subject: [Histonet] [IHCRG] Re: IHC antibody expiration question for CAP. . In-Reply-To: References: <48efa647.8702be0a.491d.6a4cSMTPIN_ADDED@mx.google.com> Message-ID: <48EF9A710200007700006112@gwmail4.harthosp.org> I have antibodies that work great 10-20 years after their expiration date. Once again, I propose that the manufactures eliminate the "expiration date" and use a "stability guarantee date". If the antibody is used after that date then the user would be responsible for validating its performance. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Jackie M O'Connor 10/10/08 3:12 PM >>> When I was making and testing antibodies for a manufacturer, we had retest dates on all the lots. As each lot reached or was past it's expiration date, we would test it against a fresh antibody lot - if it still met the exact same standard, we would extend the expiration date 3 or 6 months. Most antibodies stored at 40C I tested were still good 6 months past expiration. ALL antibodies were still good 30 days past expiration, regardless of the storage conditions. There was a strict rule about thawing frozen aliquots - no more than 3x freeze-thaw cycles, as this did damage the antibody. Throwing away frozen aliquots because they have expired is nuts. If your results are still meeting standards, it's a waste. Jackie O' "Patsy Ruegg" Sent by: ihcrg@googlegroups.com 10/10/2008 02:00 PM Please respond to pruegg@ihctech.net To , , "'ihcrg Group \(E-mail\)'" cc Subject [IHCRG] Re: IHC antibody expiration question for CAP. . Charlene, This is new to me as well. Hadi can you give us the rule where it says you can use expired abs if you freeze aliquots before they expire? Folks, do not throw away expired antibodies. The IHCRG has a reagent bank, you can send them to me. We will even provide you with a fedex act. No to use to ship the reagents. I have a refrigerator full of these reagents labs being CAP inspected have sent me. We use them in research and for teaching purposes. If any of you members of the IHCRG want to check something out we have in the bank, perhaps before you purchase it for a research project, you can do that. Just send me a message asking if I have what you need. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Henry, Charlene Sent: Friday, October 10, 2008 12:48 PM To: 'ancillarypath@mac.com'; ihcrg Group (E-mail) Subject: [IHCRG] Re: IHC antibody expiration question for CAP. . What do you do when the antibody data sheet states "do not freeze" and if freezing the antibody is accepted by CAP wouldn't the CAP Checklist state that this practice is acceptable? Also I have been to a couple CAP workshops and this question was ask. The CAP instructors stated that the antibody cannot be used past the expiration date regardless if it is frozen or stored at -20?C. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Hadi Yaziji Sent: Friday, October 10, 2008 1:28 PM To: ihcrg Group (E-mail) Subject: [IHCRG] Re: IHC antibody expiration question for CAP. . You need to educate the inspectors that it's accepted by the CAP. Most inspectors don't even know about this, because it hardly ever comes up. ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Oct 10, 2008, at 1:55 PM, Mark Tarango wrote: I'm a little skeptical too, but if this isn't allowed then it should be. On Fri, Oct 10, 2008 at 9:22 AM, Sebree Linda A. wrote: Call me skeptical but I've never heard of CAP accepting this method of shelf-life extension. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of ancillarypath@mac.com Sent: Friday, October 10, 2008 11:19 AM To: ihcrg Group (E-mail) Subject: [IHCRG] Re: IHC antibody expiration question for CAP There is a CAP-accepted way of working around this... If you aliquot the antibody before its expiration date and store it in -20 freezer, the clock is in effect "frozen" too. For example, if there are 6 months left before the antibody expires and you freeze the aliquots for 5 years, then if you thaw one of the frozen aliquots, the thawed aliquot will still be good for 6 months. Just make sure you don't thaw and re-freeze the aliquot. Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Oct 10, 2008, at 12:15 PM, Swain, Frances L wrote: Hey everyone. I use outdated antibodies I work in a research service laboratory. The reason CAP has these rules is because of patient protection. If you use an outdated antibody and the specimen comes back negative and later it was discovered that the patient had the problem that the antibody was suppose to identify. Maybe the patient dies or is permanently ill for the rest of their lives. Can you live with that? CAP is suppose to make sure that all of the labs (clinical only right now) are working under the same guidelines. They are not making these rules to make you spend more money but to protect the patient and make sure the diagnosis is as correct as can be. I agree that it is unfair to destroy antibodies that are used only once or twice before they expire. Maybe the companies preparing these antibodies should extend their expiration times? Just thought I would put my two cents worth in. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Mark Tarango Sent: Friday, October 10, 2008 11:10 AM To: themagoos@rushmore.com Cc: ihcrg@googlegroups.com Subject: [IHCRG] Re: IHC antibody expiration question for CAP The rules are the rules. We need to change them back. Mark On Fri, Oct 10, 2008 at 8:41 AM, Jason and Heather wrote: CAP question ANP.22432 states: Are all immunohistochemical reagents used within their indicated expiration dates? I am wondering what labs across the country are doing to answer this question. IHC antibodies are very expensive and can be validated on every run that is made. Some of these concentrated antibodies are diluted out so far that only a very small portion of them are used before they expire. Are some labs still using outdated antibodies? If so how do you get away with it when you are inspected by CAP? Or are you paying the high prices to throw out the majority of your antibodies so you can answer this question yes? I thank you in advance to your response. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
thermofisher.com Fri Oct 10 18:15:38 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Fri Oct 10 18:16:13 2008 Subject: [Histonet] RE: [IHCRG] IHC antibody expiration question for CAP. . In-Reply-To: <48EF9A710200007700006112@gwmail4.harthosp.org> References: <48efa647.8702be0a.491d.6a4cSMTPIN_ADDED@mx.google.com> <48EF9A710200007700006112@gwmail4.harthosp.org> Message-ID: Unfortunately, "Expiration Date" is required by FDA/IVD and European CE/IVD mark. No one is going to touch that one due to liabilities to a laboratory of re-validating "expired" reagents. Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology ThermoFisher Scientific -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Richard Cartun Sent: Friday, October 10, 2008 3:10 PM To: Jackie.O'Connor@abbott.com; pruegg@ihctech.net; Histonet Cc: 'ihcrg Group (E-mail)'; ancillarypath@mac.com; charlene.henry@stjude.org Subject: [IHCRG] IHC antibody expiration question for CAP. . I have antibodies that work great 10-20 years after their expiration date. Once again, I propose that the manufactures eliminate the "expiration date" and use a "stability guarantee date". If the antibody is used after that date then the user would be responsible for validating its performance. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Jackie M O'Connor 10/10/08 3:12 PM >>> When I was making and testing antibodies for a manufacturer, we had retest dates on all the lots. As each lot reached or was past it's expiration date, we would test it against a fresh antibody lot - if it still met the exact same standard, we would extend the expiration date 3 or 6 months. Most antibodies stored at 40C I tested were still good 6 months past expiration. ALL antibodies were still good 30 days past expiration, regardless of the storage conditions. There was a strict rule about thawing frozen aliquots - no more than 3x freeze-thaw cycles, as this did damage the antibody. Throwing away frozen aliquots because they have expired is nuts. If your results are still meeting standards, it's a waste. Jackie O' "Patsy Ruegg" Sent by: ihcrg@googlegroups.com 10/10/2008 02:00 PM Please respond to pruegg@ihctech.net To , , "'ihcrg Group \(E-mail\)'" cc Subject [IHCRG] Re: IHC antibody expiration question for CAP. . Charlene, This is new to me as well. Hadi can you give us the rule where it says you can use expired abs if you freeze aliquots before they expire? Folks, do not throw away expired antibodies. The IHCRG has a reagent bank, you can send them to me. We will even provide you with a fedex act. No to use to ship the reagents. I have a refrigerator full of these reagents labs being CAP inspected have sent me. We use them in research and for teaching purposes. If any of you members of the IHCRG want to check something out we have in the bank, perhaps before you purchase it for a research project, you can do that. Just send me a message asking if I have what you need. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Henry, Charlene Sent: Friday, October 10, 2008 12:48 PM To: 'ancillarypath@mac.com'; ihcrg Group (E-mail) Subject: [IHCRG] Re: IHC antibody expiration question for CAP. . What do you do when the antibody data sheet states "do not freeze" and if freezing the antibody is accepted by CAP wouldn't the CAP Checklist state that this practice is acceptable? Also I have been to a couple CAP workshops and this question was ask. The CAP instructors stated that the antibody cannot be used past the expiration date regardless if it is frozen or stored at -20?C. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Hadi Yaziji Sent: Friday, October 10, 2008 1:28 PM To: ihcrg Group (E-mail) Subject: [IHCRG] Re: IHC antibody expiration question for CAP. . You need to educate the inspectors that it's accepted by the CAP. Most inspectors don't even know about this, because it hardly ever comes up. ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Oct 10, 2008, at 1:55 PM, Mark Tarango wrote: I'm a little skeptical too, but if this isn't allowed then it should be. On Fri, Oct 10, 2008 at 9:22 AM, Sebree Linda A. wrote: Call me skeptical but I've never heard of CAP accepting this method of shelf-life extension. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of ancillarypath@mac.com Sent: Friday, October 10, 2008 11:19 AM To: ihcrg Group (E-mail) Subject: [IHCRG] Re: IHC antibody expiration question for CAP There is a CAP-accepted way of working around this... If you aliquot the antibody before its expiration date and store it in -20 freezer, the clock is in effect "frozen" too. For example, if there are 6 months left before the antibody expires and you freeze the aliquots for 5 years, then if you thaw one of the frozen aliquots, the thawed aliquot will still be good for 6 months. Just make sure you don't thaw and re-freeze the aliquot. Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Oct 10, 2008, at 12:15 PM, Swain, Frances L wrote: Hey everyone. I use outdated antibodies I work in a research service laboratory. The reason CAP has these rules is because of patient protection. If you use an outdated antibody and the specimen comes back negative and later it was discovered that the patient had the problem that the antibody was suppose to identify. Maybe the patient dies or is permanently ill for the rest of their lives. Can you live with that? CAP is suppose to make sure that all of the labs (clinical only right now) are working under the same guidelines. They are not making these rules to make you spend more money but to protect the patient and make sure the diagnosis is as correct as can be. I agree that it is unfair to destroy antibodies that are used only once or twice before they expire. Maybe the companies preparing these antibodies should extend their expiration times? Just thought I would put my two cents worth in. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Mark Tarango Sent: Friday, October 10, 2008 11:10 AM To: themagoos@rushmore.com Cc: ihcrg@googlegroups.com Subject: [IHCRG] Re: IHC antibody expiration question for CAP The rules are the rules. We need to change them back. Mark On Fri, Oct 10, 2008 at 8:41 AM, Jason and Heather wrote: CAP question ANP.22432 states: Are all immunohistochemical reagents used within their indicated expiration dates? I am wondering what labs across the country are doing to answer this question. IHC antibodies are very expensive and can be validated on every run that is made. Some of these concentrated antibodies are diluted out so far that only a very small portion of them are used before they expire. Are some labs still using outdated antibodies? If so how do you get away with it when you are inspected by CAP? Or are you paying the high prices to throw out the majority of your antibodies so you can answer this question yes? I thank you in advance to your response. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
sbcglobal.net Fri Oct 10 19:27:09 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Oct 10 19:27:22 2008 Subject: [Histonet] RE: [IHCRG] IHC antibody expiration question for CAP. . In-Reply-To: Message-ID: <001a01c92b38$195abdf0$0202a8c0@HPPav2> In addition, ruling is from Department of Health and Human Services (DHHS), Center for Medicaid and Medicare Services (CMS), 42 CFR 493.1252(c) http://edocket.access.gpo.gov/cfr_2006/octqtr/pdf/42cfr493.1253.pdf So it's bigger than CAP. It's the US government. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospitals Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Friday, October 10, 2008 7:16 PM To: 'Histonet' Cc: 'ihcrg Group (E-mail)' Subject: [Histonet] RE: [IHCRG] IHC antibody expiration question for CAP. . Unfortunately, "Expiration Date" is required by FDA/IVD and European CE/IVD mark. No one is going to touch that one due to liabilities to a laboratory of re-validating "expired" reagents. Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology ThermoFisher Scientific -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Richard Cartun Sent: Friday, October 10, 2008 3:10 PM To: Jackie.O'Connor@abbott.com; pruegg@ihctech.net; Histonet Cc: 'ihcrg Group (E-mail)'; ancillarypath@mac.com; charlene.henry@stjude.org Subject: [IHCRG] IHC antibody expiration question for CAP. . I have antibodies that work great 10-20 years after their expiration date. Once again, I propose that the manufactures eliminate the "expiration date" and use a "stability guarantee date". If the antibody is used after that date then the user would be responsible for validating its performance. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Jackie M O'Connor 10/10/08 3:12 PM >>> When I was making and testing antibodies for a manufacturer, we had retest dates on all the lots. As each lot reached or was past it's expiration date, we would test it against a fresh antibody lot - if it still met the exact same standard, we would extend the expiration date 3 or 6 months. Most antibodies stored at 40C I tested were still good 6 months past expiration. ALL antibodies were still good 30 days past expiration, regardless of the storage conditions. There was a strict rule about thawing frozen aliquots - no more than 3x freeze-thaw cycles, as this did damage the antibody. Throwing away frozen aliquots because they have expired is nuts. If your results are still meeting standards, it's a waste. Jackie O' "Patsy Ruegg" Sent by: ihcrg@googlegroups.com 10/10/2008 02:00 PM Please respond to pruegg@ihctech.net To , , "'ihcrg Group \(E-mail\)'" cc Subject [IHCRG] Re: IHC antibody expiration question for CAP. . Charlene, This is new to me as well. Hadi can you give us the rule where it says you can use expired abs if you freeze aliquots before they expire? Folks, do not throw away expired antibodies. The IHCRG has a reagent bank, you can send them to me. We will even provide you with a fedex act. No to use to ship the reagents. I have a refrigerator full of these reagents labs being CAP inspected have sent me. We use them in research and for teaching purposes. If any of you members of the IHCRG want to check something out we have in the bank, perhaps before you purchase it for a research project, you can do that. Just send me a message asking if I have what you need. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Henry, Charlene Sent: Friday, October 10, 2008 12:48 PM To: 'ancillarypath@mac.com'; ihcrg Group (E-mail) Subject: [IHCRG] Re: IHC antibody expiration question for CAP. . What do you do when the antibody data sheet states "do not freeze" and if freezing the antibody is accepted by CAP wouldn't the CAP Checklist state that this practice is acceptable? Also I have been to a couple CAP workshops and this question was ask. The CAP instructors stated that the antibody cannot be used past the expiration date regardless if it is frozen or stored at -20?C. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Hadi Yaziji Sent: Friday, October 10, 2008 1:28 PM To: ihcrg Group (E-mail) Subject: [IHCRG] Re: IHC antibody expiration question for CAP. . You need to educate the inspectors that it's accepted by the CAP. Most inspectors don't even know about this, because it hardly ever comes up. ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Oct 10, 2008, at 1:55 PM, Mark Tarango wrote: I'm a little skeptical too, but if this isn't allowed then it should be. On Fri, Oct 10, 2008 at 9:22 AM, Sebree Linda A. wrote: Call me skeptical but I've never heard of CAP accepting this method of shelf-life extension. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of ancillarypath@mac.com Sent: Friday, October 10, 2008 11:19 AM To: ihcrg Group (E-mail) Subject: [IHCRG] Re: IHC antibody expiration question for CAP There is a CAP-accepted way of working around this... If you aliquot the antibody before its expiration date and store it in -20 freezer, the clock is in effect "frozen" too. For example, if there are 6 months left before the antibody expires and you freeze the aliquots for 5 years, then if you thaw one of the frozen aliquots, the thawed aliquot will still be good for 6 months. Just make sure you don't thaw and re-freeze the aliquot. Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Oct 10, 2008, at 12:15 PM, Swain, Frances L wrote: Hey everyone. I use outdated antibodies I work in a research service laboratory. The reason CAP has these rules is because of patient protection. If you use an outdated antibody and the specimen comes back negative and later it was discovered that the patient had the problem that the antibody was suppose to identify. Maybe the patient dies or is permanently ill for the rest of their lives. Can you live with that? CAP is suppose to make sure that all of the labs (clinical only right now) are working under the same guidelines. They are not making these rules to make you spend more money but to protect the patient and make sure the diagnosis is as correct as can be. I agree that it is unfair to destroy antibodies that are used only once or twice before they expire. Maybe the companies preparing these antibodies should extend their expiration times? Just thought I would put my two cents worth in. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Mark Tarango Sent: Friday, October 10, 2008 11:10 AM To: themagoos@rushmore.com Cc: ihcrg@googlegroups.com Subject: [IHCRG] Re: IHC antibody expiration question for CAP The rules are the rules. We need to change them back. Mark On Fri, Oct 10, 2008 at 8:41 AM, Jason and Heather wrote: CAP question ANP.22432 states: Are all immunohistochemical reagents used within their indicated expiration dates? I am wondering what labs across the country are doing to answer this question. IHC antibodies are very expensive and can be validated on every run that is made. Some of these concentrated antibodies are diluted out so far that only a very small portion of them are used before they expire. Are some labs still using outdated antibodies? If so how do you get away with it when you are inspected by CAP? Or are you paying the high prices to throw out the majority of your antibodies so you can answer this question yes? I thank you in advance to your response. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
hotmail.com Fri Oct 10 20:59:28 2008 From: debbie_hogg <@t> hotmail.com (Debbie Hogg) Date: Fri Oct 10 20:59:33 2008 Subject: [Histonet] PAS Control blocks needed! Message-ID: Hello: Does anyone have any spare liver blocks that they could donate? Our hospital does not perform that procedure and we have reached a zero level. Please contact me and I will send my hospitals address to you. I will gladly pay for the blocks and for postage to my place of work (Ontario, Canada). Thank you for reading my request (I know that there are many listings to go through!!). Debbie _________________________________________________________________ From lpwenk <@t> sbcglobal.net Fri Oct 10 21:33:27 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Oct 10 21:33:40 2008 Subject: [Histonet] PAS Control blocks needed! In-Reply-To: Message-ID: <001e01c92b49$be4c05b0$0202a8c0@HPPav2> Any chance you can get cervix? Ectocervix has glycogen in it. Just a suggestion. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Hogg Sent: Friday, October 10, 2008 9:59 PM To: Histo Net Subject: [Histonet] PAS Control blocks needed! Hello: Does anyone have any spare liver blocks that they could donate? Our hospital does not perform that procedure and we have reached a zero level. Please contact me and I will send my hospitals address to you. I will gladly pay for the blocks and for postage to my place of work (Ontario, Canada). Thank you for reading my request (I know that there are many listings to go through!!). Debbie _________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Oct 11 04:15:44 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Oct 11 04:16:09 2008 Subject: AW: [Histonet] histology ink washing off In-Reply-To: <000c01c92b17$354b76b0$3ba62f0a@DHGLABC99E93DF> Message-ID: <39706966F1BD47EB966699DCF77ED1C0@dielangs.at> We dry and heat the tissue surface with a hair-dryer at the grossing. It sounds a little bit rigid, but it works and the tissue margins are easily seen in the slide. The excess dye also will be solved in the NBF, but there sticks enough on the tissuemargins. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Leslie Chen Gesendet: Freitag, 10. Oktober 2008 22:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] histology ink washing off Hello, I am having a problem with histology tissue marking ink washing off specimens in water, PFA, or 70% alcohol. This is before the tissue even gets processed for paraffin embedding. Does anyone have any suggestions on how to keep the ink from coming off? Thank you. Leslie ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Oct 11 04:16:55 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Oct 11 04:17:20 2008 Subject: AW: [Histonet] Re: Section thickness In-Reply-To: <18391.71280.qm@web90305.mail.mud.yahoo.com> Message-ID: <07DDCB33F0CB4BBBAE9ED45D77516A0F@dielangs.at> The same here. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Shelly Coker Gesendet: Freitag, 10. Oktober 2008 23:34 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: Section thickness At the labs I have worked at we cut routine tissue at 3um, lymph nodes and kidney biopsies at 2um, bone marrow clots and biopsies at 1 um. Hope this helps, Shelly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From susanbachus <@t> verizon.net Sun Oct 12 02:26:08 2008 From: susanbachus <@t> verizon.net (Susan Bachus) Date: Sun Oct 12 02:26:12 2008 Subject: [Histonet] histology ink washing off References: <39706966F1BD47EB966699DCF77ED1C0@dielangs.at> Message-ID: Back in the old days we used to use "india ink" to label slides--so I don't know whether it would also work on tissue (as opposed to glass), but it might be worth a try. Susan ----- Original Message ----- From: "Gudrun Lang" To: "'Leslie Chen'" Cc: Sent: Saturday, October 11, 2008 5:15 AM Subject: AW: [Histonet] histology ink washing off We dry and heat the tissue surface with a hair-dryer at the grossing. It sounds a little bit rigid, but it works and the tissue margins are easily seen in the slide. The excess dye also will be solved in the NBF, but there sticks enough on the tissuemargins. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Leslie Chen Gesendet: Freitag, 10. Oktober 2008 22:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] histology ink washing off Hello, I am having a problem with histology tissue marking ink washing off specimens in water, PFA, or 70% alcohol. This is before the tissue even gets processed for paraffin embedding. Does anyone have any suggestions on how to keep the ink from coming off? Thank you. Leslie ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Sun Oct 12 08:17:36 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Oct 12 08:17:50 2008 Subject: [Histonet] RE: [IHCRG] IHC antibody expiration question for CAP. . In-Reply-To: References: <48efa647.8702be0a.491d.6a4cSMTPIN_ADDED@mx.google.com> <48EF9A710200007700006112@gwmail4.harthosp.org> Message-ID: <48F1C0B0020000770000614B@gwmail4.harthosp.org> I realize that this is a CLIA/FDA mandate, but, like many other things in our world today, it's time for change. I know that members of the CAP have tried to get this rule changed, but their hands are tied by Washington - too much government, too much bureaucracy, too much "red tape". Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Morken, Tim" 10/10/08 7:15 PM >>> Unfortunately, "Expiration Date" is required by FDA/IVD and European CE/IVD mark. No one is going to touch that one due to liabilities to a laboratory of re-validating "expired" reagents. Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology ThermoFisher Scientific -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Richard Cartun Sent: Friday, October 10, 2008 3:10 PM To: Jackie.O'Connor@abbott.com; pruegg@ihctech.net; Histonet Cc: 'ihcrg Group (E-mail)'; ancillarypath@mac.com; charlene.henry@stjude.org Subject: [IHCRG] IHC antibody expiration question for CAP. . I have antibodies that work great 10-20 years after their expiration date. Once again, I propose that the manufactures eliminate the "expiration date" and use a "stability guarantee date". If the antibody is used after that date then the user would be responsible for validating its performance. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Jackie M O'Connor 10/10/08 3:12 PM >>> When I was making and testing antibodies for a manufacturer, we had retest dates on all the lots. As each lot reached or was past it's expiration date, we would test it against a fresh antibody lot - if it still met the exact same standard, we would extend the expiration date 3 or 6 months. Most antibodies stored at 40C I tested were still good 6 months past expiration. ALL antibodies were still good 30 days past expiration, regardless of the storage conditions. There was a strict rule about thawing frozen aliquots - no more than 3x freeze-thaw cycles, as this did damage the antibody. Throwing away frozen aliquots because they have expired is nuts. If your results are still meeting standards, it's a waste. Jackie O' "Patsy Ruegg" Sent by: ihcrg@googlegroups.com 10/10/2008 02:00 PM Please respond to pruegg@ihctech.net To , , "'ihcrg Group \(E-mail\)'" cc Subject [IHCRG] Re: IHC antibody expiration question for CAP. . Charlene, This is new to me as well. Hadi can you give us the rule where it says you can use expired abs if you freeze aliquots before they expire? Folks, do not throw away expired antibodies. The IHCRG has a reagent bank, you can send them to me. We will even provide you with a fedex act. No to use to ship the reagents. I have a refrigerator full of these reagents labs being CAP inspected have sent me. We use them in research and for teaching purposes. If any of you members of the IHCRG want to check something out we have in the bank, perhaps before you purchase it for a research project, you can do that. Just send me a message asking if I have what you need. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Henry, Charlene Sent: Friday, October 10, 2008 12:48 PM To: 'ancillarypath@mac.com'; ihcrg Group (E-mail) Subject: [IHCRG] Re: IHC antibody expiration question for CAP. . What do you do when the antibody data sheet states "do not freeze" and if freezing the antibody is accepted by CAP wouldn't the CAP Checklist state that this practice is acceptable? Also I have been to a couple CAP workshops and this question was ask. The CAP instructors stated that the antibody cannot be used past the expiration date regardless if it is frozen or stored at -20?C. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Hadi Yaziji Sent: Friday, October 10, 2008 1:28 PM To: ihcrg Group (E-mail) Subject: [IHCRG] Re: IHC antibody expiration question for CAP. . You need to educate the inspectors that it's accepted by the CAP. Most inspectors don't even know about this, because it hardly ever comes up. ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Oct 10, 2008, at 1:55 PM, Mark Tarango wrote: I'm a little skeptical too, but if this isn't allowed then it should be. On Fri, Oct 10, 2008 at 9:22 AM, Sebree Linda A. wrote: Call me skeptical but I've never heard of CAP accepting this method of shelf-life extension. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of ancillarypath@mac.com Sent: Friday, October 10, 2008 11:19 AM To: ihcrg Group (E-mail) Subject: [IHCRG] Re: IHC antibody expiration question for CAP There is a CAP-accepted way of working around this... If you aliquot the antibody before its expiration date and store it in -20 freezer, the clock is in effect "frozen" too. For example, if there are 6 months left before the antibody expires and you freeze the aliquots for 5 years, then if you thaw one of the frozen aliquots, the thawed aliquot will still be good for 6 months. Just make sure you don't thaw and re-freeze the aliquot. Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Oct 10, 2008, at 12:15 PM, Swain, Frances L wrote: Hey everyone. I use outdated antibodies I work in a research service laboratory. The reason CAP has these rules is because of patient protection. If you use an outdated antibody and the specimen comes back negative and later it was discovered that the patient had the problem that the antibody was suppose to identify. Maybe the patient dies or is permanently ill for the rest of their lives. Can you live with that? CAP is suppose to make sure that all of the labs (clinical only right now) are working under the same guidelines. They are not making these rules to make you spend more money but to protect the patient and make sure the diagnosis is as correct as can be. I agree that it is unfair to destroy antibodies that are used only once or twice before they expire. Maybe the companies preparing these antibodies should extend their expiration times? Just thought I would put my two cents worth in. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Mark Tarango Sent: Friday, October 10, 2008 11:10 AM To: themagoos@rushmore.com Cc: ihcrg@googlegroups.com Subject: [IHCRG] Re: IHC antibody expiration question for CAP The rules are the rules. We need to change them back. Mark On Fri, Oct 10, 2008 at 8:41 AM, Jason and Heather wrote: CAP question ANP.22432 states: Are all immunohistochemical reagents used within their indicated expiration dates? I am wondering what labs across the country are doing to answer this question. IHC antibodies are very expensive and can be validated on every run that is made. Some of these concentrated antibodies are diluted out so far that only a very small portion of them are used before they expire. Are some labs still using outdated antibodies? If so how do you get away with it when you are inspected by CAP? Or are you paying the high prices to throw out the majority of your antibodies so you can answer this question yes? I thank you in advance to your response. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
netzero.net Sun Oct 12 09:24:40 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Sun Oct 12 09:24:43 2008 Subject: [Histonet] histology ink washing off In-Reply-To: References: <39706966F1BD47EB966699DCF77ED1C0@dielangs.at> Message-ID: India ink is commonly used to mark margins on breast and skin biopsies. Dry the surface with a paper towel, then dab on India ink with a cotton swab and then dip the specimen (with or without blotting off the excess ink) in either 5% acetic acid (aqueous) or Bouin's fluid before cutting sections to submit in cassettes. The acid 'fixes' the dye to the surface so it will not run into the deeper recesses of the specimen. This method can also be used to fix colored inks to the margin when medial/lateral and superior/inferior orientation is important. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Bachus Sent: Sunday, October 12, 2008 12:26 AM To: lchen@mednet.ucla.edu Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] histology ink washing off Back in the old days we used to use "india ink" to label slides--so I don't know whether it would also work on tissue (as opposed to glass), but it might be worth a try. Susan ----- Original Message ----- From: "Gudrun Lang" To: "'Leslie Chen'" Cc: Sent: Saturday, October 11, 2008 5:15 AM Subject: AW: [Histonet] histology ink washing off We dry and heat the tissue surface with a hair-dryer at the grossing. It sounds a little bit rigid, but it works and the tissue margins are easily seen in the slide. The excess dye also will be solved in the NBF, but there sticks enough on the tissuemargins. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Leslie Chen Gesendet: Freitag, 10. Oktober 2008 22:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] histology ink washing off Hello, I am having a problem with histology tissue marking ink washing off specimens in water, PFA, or 70% alcohol. This is before the tissue even gets processed for paraffin embedding. Does anyone have any suggestions on how to keep the ink from coming off? Thank you. Leslie ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.8.0/1719 - Release Date: 10/10/2008 4:08 PM From Rcartun <@t> harthosp.org Sun Oct 12 10:31:16 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Oct 12 10:31:28 2008 Subject: [Histonet] Dymo slide label printer Message-ID: <48F1E0040200007700006192@gwmail4.harthosp.org> In the past, someone from Histonet was nice enough to send me the product number for slide labels for the Dymo printer. Guess what? I can't find it. Hopefully, someone can send me that information? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From RSRICHMOND <@t> aol.com Sun Oct 12 13:08:55 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Sun Oct 12 13:08:59 2008 Subject: [Histonet] Re: histology ink washing off Message-ID: Inks - both ordinary india ink and colored inks - used for marking histology specimens will usually stay on quite satisfactorily if you make sure to blot the specimen dry before inking. I never anything to fix the ink to the specimen. 2% to 5% acetic acid is simple to use if you or your pathologist want it. Don't use acetone (flammable) or Bouin's fixative (toxic and messy). Ink doesn't adhere well to cauterized tissue surfaces. It adheres reasonably well to cauterized breast tissue, not at all to the cauterized surfaces of LEEP specimens of the cervix (where the cauterized surfaces themselves are an adequate guide to the margins for the microscopist). Ordinary india ink - most of what the pathologist uses - is easily bought at artist's or craft supply stores, much cheaper and in more convenient containers than you get from medical supply houses. When multiple colors are needed, some people use tattoo inks (cheap, and available in numerous colors), but most use inks made specially for this purpose. I prefer the Davidson marking inks (now available from ordinary lab vendors like whatever Thermo and Cardinal are called this week. I have no commercial connection to this product.) Inks dry out and go bad if the containers aren't promptly capped after use. Pathologists tend to forget this, and the histotech who assists the pathologist should make sure that the caps are replaced. The little dye capsules are an abomination - they squirt and flood the specimen and your clothes with unwanted dye. Bob Richmond Samurai Pathologist Knoxville TN From darkdaym <@t> comcast.net Sun Oct 12 13:44:01 2008 From: darkdaym <@t> comcast.net (Mark Ray) Date: Sun Oct 12 13:44:36 2008 Subject: [Histonet] Re: histology ink washing off In-Reply-To: References: Message-ID: <48F24571.2030109@comcast.net> Most institutions have a discount contract with a large office supply company. Borrow a catalog from the office staff. That's usually the cheapest deal on india ink and you don't have to go to an outside vendor that your purchasing department never heard of. Robert Richmond wrote: > Inks - both ordinary india ink and colored inks - used for marking > histology specimens will usually stay on quite satisfactorily if you > make sure to blot the specimen dry before inking. I never anything to > fix the ink to the specimen. 2% to 5% acetic acid is simple to use if > you or your pathologist want it. Don't use acetone (flammable) or > Bouin's fixative (toxic and messy). > > Ink doesn't adhere well to cauterized tissue surfaces. It adheres > reasonably well to cauterized breast tissue, not at all to the > cauterized surfaces of LEEP specimens of the cervix (where the > cauterized surfaces themselves are an adequate guide to the margins > for the microscopist). > > Ordinary india ink - most of what the pathologist uses - is easily > bought at artist's or craft supply stores, much cheaper and in more > convenient containers than you get from medical supply houses. When > multiple colors are needed, some people use tattoo inks (cheap, and > available in numerous colors), but most use inks made specially for > this purpose. I prefer the Davidson marking inks (now available from > ordinary lab vendors like whatever Thermo and Cardinal are called this > week. I have no commercial connection to this product.) > > Inks dry out and go bad if the containers aren't promptly capped after > use. Pathologists tend to forget this, and the histotech who assists > the pathologist should make sure that the caps are replaced. > > The little dye capsules are an abomination - they squirt and flood the > specimen and your clothes with unwanted dye. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From TMcNemar <@t> lmhealth.org Mon Oct 13 05:09:07 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Oct 13 05:08:55 2008 Subject: [Histonet] Dymo slide label printer In-Reply-To: <48F1E0040200007700006192@gwmail4.harthosp.org> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5BE@lmhsmail.lmhealth.org> Dymo 1"x1" labels - cat#30332 Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Sunday, October 12, 2008 11:31 AM To: Histonet Subject: [Histonet] Dymo slide label printer In the past, someone from Histonet was nice enough to send me the product number for slide labels for the Dymo printer. Guess what? I can't find it. Hopefully, someone can send me that information? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arsenn <@t> hsh.org Mon Oct 13 06:04:10 2008 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Mon Oct 13 06:04:16 2008 Subject: [Histonet] Expiration dates Message-ID: Hello all, My supervisor wanted me to ask everyone who's posting about the expiration dates of antibodies: How can you use expired antibodies on a stainer such as a Benchmark-which will not allow you to start the run due to expiration dates on the containers? Thanks!! Amy Senn Camp Hill, PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From LSebree <@t> uwhealth.org Mon Oct 13 07:53:42 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Mon Oct 13 07:53:51 2008 Subject: [Histonet] Expiration dates In-Reply-To: Message-ID: I the case of VMS instrumentation, we would be talking about user-fillable dispensers that you would fill with non-Ventana antibodies Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Monday, October 13, 2008 6:04 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Expiration dates Hello all, My supervisor wanted me to ask everyone who's posting about the expiration dates of antibodies: How can you use expired antibodies on a stainer such as a Benchmark-which will not allow you to start the run due to expiration dates on the containers? Thanks!! Amy Senn Camp Hill, PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Mon Oct 13 09:30:15 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Oct 13 09:30:36 2008 Subject: [Histonet] Modified Davidsons Vendor in Europe In-Reply-To: <48EB428B0200007700005F81@gwmail6.harthosp.org> Message-ID: I'm in Germany this week, and wondering if there is a vendor in Europe for Modified Davidson's solution. Any clues? Thanks. Jackie O' From marktarango <@t> gmail.com Mon Oct 13 10:46:22 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Oct 13 10:46:30 2008 Subject: [Histonet] Expiration dates In-Reply-To: References: Message-ID: <5b6eb13e0810130846g22311d6fj49146918c34f9261@mail.gmail.com> Throw a prep kit label on the primary antibody and change the protocol's primary antibody to the read "prep kit 1" or whatever prep kit it is. You didn't hear it from me. On Mon, Oct 13, 2008 at 4:04 AM, Senn, Amy R wrote: > Hello all, > > > > My supervisor wanted me to ask everyone who's posting about the > expiration dates of antibodies: How can you use expired antibodies on a > stainer such as a Benchmark-which will not allow you to start the run > due to expiration dates on the containers? > > > > > > Thanks!! > > > > Amy Senn > > Camp Hill, PA > > > > Confidentiality Disclaimer: The information contained in this communication > may be confidential, > is intended for the use of the recipient named above, and may be legally > privileged.If the reader > of this message is not the intended recipient, you are hereby notified that > any dissemination, > distribution, or copying of this communication, or any of its contents, is > strictly prohibited. If you > received this communication in error, please resend this communication to > the sender and delete > the original message and any copy of it from your computer system. Thank > You > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Sarah.Blachford <@t> ngh.nhs.uk Mon Oct 13 11:16:39 2008 From: Sarah.Blachford <@t> ngh.nhs.uk (Blachford, Sarah - Pathology) Date: Mon Oct 13 11:16:54 2008 Subject: [Histonet] cold plates Message-ID: Dear All Does anyone know where you can purchase bench top cold plates. We would like small individual ones so not too big. Srah Blachford Northampton General Hospital Northampton General Hospital NHS Trust Cliftonville, Northampton NN1 5BD This e-mail may contain confidential information and/or copyright material and is intended for the use of the addressee only. Any unauthorised use may be unlawful. The contents of this e-mail may be subject to public disclosure under the NHS Code of Openness or the Freedom of Information Act 2000. Unless legally exempt, the confidentiality of the message and your reply cannot be guaranteed. If you receive this e-mail by mistake, please advise the sender immediately. Thank you. From shive003 <@t> umn.edu Mon Oct 13 11:09:37 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Oct 13 11:23:25 2008 Subject: [Histonet] Microwave ovens for IHC Message-ID: Hi, The microwave I use for HIER in IHC just died this morning, and so I'm scrambling to find a replacement. I guess this is when I bite the bullet and purchase one for laboratory use. I see that EBS has 3 models available (H1850, H2250, H2850) , in various price ranges. Does anyone have experience with their products, and if so, which model works well for you in performing antigen retrieval, and is the instrument calibratable? Please respond to me personally. Thanks. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From LSebree <@t> uwhealth.org Mon Oct 13 11:37:59 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Mon Oct 13 11:38:08 2008 Subject: [Histonet] Expiration dates In-Reply-To: <5b6eb13e0810130846g22311d6fj49146918c34f9261@mail.gmail.com> Message-ID: ....and we all do it Mark! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Monday, October 13, 2008 10:46 AM To: Senn, Amy R Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Expiration dates Throw a prep kit label on the primary antibody and change the protocol's primary antibody to the read "prep kit 1" or whatever prep kit it is. You didn't hear it from me. On Mon, Oct 13, 2008 at 4:04 AM, Senn, Amy R wrote: > Hello all, > > > > My supervisor wanted me to ask everyone who's posting about the > expiration dates of antibodies: How can you use expired antibodies on a > stainer such as a Benchmark-which will not allow you to start the run > due to expiration dates on the containers? > > > > > > Thanks!! > > > > Amy Senn > > Camp Hill, PA > > > > Confidentiality Disclaimer: The information contained in this communication > may be confidential, > is intended for the use of the recipient named above, and may be legally > privileged.If the reader > of this message is not the intended recipient, you are hereby notified that > any dissemination, > distribution, or copying of this communication, or any of its contents, is > strictly prohibited. If you > received this communication in error, please resend this communication to > the sender and delete > the original message and any copy of it from your computer system. Thank > You > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SEsparza <@t> seton.org Mon Oct 13 11:56:15 2008 From: SEsparza <@t> seton.org (Esparza, Sandra) Date: Mon Oct 13 11:59:21 2008 Subject: [Histonet] cold plates References: Message-ID: <3D79F47DC92B204F9E5D35C885DFC5CB01003C08@AUSEX2VS1.seton.org> I use the Leica EG 1130. It works great, gets cold fast and measures about 18 inches wide by 24 inches deep. (About 14 inches is actually cooling plate so 18 inches wide and 14 inches deep.) I'm not sure of the price check with your Leica rep. Hope this helps, Sandra HT (ASCP) Dell Children's Hospital Austin, Texas -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blachford, Sarah - Pathology Sent: Monday, October 13, 2008 11:17 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] cold plates Dear All Does anyone know where you can purchase bench top cold plates. We would like small individual ones so not too big. Srah Blachford Northampton General Hospital Northampton General Hospital NHS Trust Cliftonville, Northampton NN1 5BD This e-mail may contain confidential information and/or copyright material and is intended for the use of the addressee only. Any unauthorised use may be unlawful. The contents of this e-mail may be subject to public disclosure under the NHS Code of Openness or the Freedom of Information Act 2000. Unless legally exempt, the confidentiality of the message and your reply cannot be guaranteed. If you receive this e-mail by mistake, please advise the sender immediately. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Mon Oct 13 12:17:49 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Oct 13 12:17:53 2008 Subject: [Histonet] restarting DAB reaction Message-ID: Has anyone ever tried to continue a DAB reaction after the slides have been washed and coverslipped? I can't think of a reason why this wouldn't work, since we just wash the slides in PBS and water after the DAB step. We use a vectastain kit for this--would we need to add more of the ABC reagent (after removing the coverslips and washing in PBS) and then do DAB? Emily -- the velocity of time turns her voice into sugar water http://www.youtube.com/watch?v=jNA6zzoObxg From michelle.steinkrauss <@t> novartis.com Mon Oct 13 12:35:53 2008 From: michelle.steinkrauss <@t> novartis.com (michelle.steinkrauss@novartis.com) Date: Mon Oct 13 12:36:02 2008 Subject: [Histonet] Michelle Steinkrauss is out of the office. Message-ID: I will be out of the office starting 10/13/2008 and will not return until 10/14/2008. If you require immediate assistance, please contact Michelle Broome at x 47477. From maria.geraci-erck <@t> spcorp.com Mon Oct 13 12:55:41 2008 From: maria.geraci-erck <@t> spcorp.com (Geraci-Erck, Maria) Date: Mon Oct 13 12:55:46 2008 Subject: [Histonet] Antibody for monkey tissue to anti-nuclear antibody (ANA's) Message-ID: Hello Histonetters, Can anyone recommend an anti-nuclear antibody to label paraffin embedded, formalin fixed monkey tissue? A researcher of ours is looking for general immune response/inflammation. Maria Maria Geraci-Erck Schering-Plough Research Institute Special Techniques Laboratory 556 Morris Avenue, Bldg. S-12 Summit, New Jersey 07901-1002 Phone: (908) 473-4284 Fax: (908) 473-4420 maria.geraci-erck@spcorp.com ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From RSRICHMOND <@t> aol.com Mon Oct 13 13:16:52 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Mon Oct 13 13:17:01 2008 Subject: [Histonet] Re: Modified Davidsons Vendor in Europe Message-ID: Jackie O'Connor wants to know if there is a vendor for modified Davidson's fixative in Europe. three parts tap water, three parts ethyl alcohol or reagent alcohol, two parts 37% formaldehyde, one part glacial acetic acid I would hope that the level of scientific education among European histologists is such that they would still be allowed to make this simple formulation themselves. Bob Richmond Samurai Pathologist Knoxville, Tennessee From alexandra.meinl <@t> gmail.com Mon Oct 13 13:59:35 2008 From: alexandra.meinl <@t> gmail.com (Alexandra Meinl) Date: Mon Oct 13 13:59:40 2008 Subject: [Histonet] Re: Modified Davidsons Vendor in Europe In-Reply-To: References: Message-ID: Usually we manage it to make mDF. But Diagonal has it (http://www.diagonal-shop.de), and VWR also. Alexandra 2008/10/13 Robert Richmond > Jackie O'Connor wants to know if there is a vendor for modified > Davidson's fixative in Europe. > > three parts tap water, three parts ethyl alcohol or reagent alcohol, > two parts 37% formaldehyde, one part glacial acetic acid > > I would hope that the level of scientific education among European > histologists is such that they would still be allowed to make this > simple formulation themselves. > > Bob Richmond > Samurai Pathologist > Knoxville, Tennessee > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lchen <@t> mednet.ucla.edu Mon Oct 13 14:19:00 2008 From: lchen <@t> mednet.ucla.edu (Leslie Chen) Date: Mon Oct 13 14:19:15 2008 Subject: [Histonet] histology ink washing off Message-ID: <003b01c92d68$92096df0$3ba62f0a@DHGLABC99E93DF> Thank you everyone for your help. It seems the consensus is to use acetic acid as a mordant which worked. I have tried just blotting the tissue, but it never seems to completely "dry". The tissue has been fixed in gluteraldehyde and stained with the LacZ protocol and then post-fixed with PFA. This may be why the tissue never seems to dry. Has anyone experienced this before with LacZ staining? This has also caused some problems when frozen sectioning. Thank you! Leslie ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From rjbuesa <@t> yahoo.com Mon Oct 13 14:31:44 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 13 14:31:47 2008 Subject: [Histonet] restarting DAB reaction In-Reply-To: Message-ID: <465616.22158.qm@web65706.mail.ac4.yahoo.com> I also do not see any reason why you cannot hydrate the sections again and add the DAB. It should work. Ren? J. --- On Mon, 10/13/08, Emily Sours wrote: From: Emily Sours Subject: [Histonet] restarting DAB reaction To: histonet@lists.utsouthwestern.edu Date: Monday, October 13, 2008, 1:17 PM Has anyone ever tried to continue a DAB reaction after the slides have been washed and coverslipped? I can't think of a reason why this wouldn't work, since we just wash the slides in PBS and water after the DAB step. We use a vectastain kit for this--would we need to add more of the ABC reagent (after removing the coverslips and washing in PBS) and then do DAB? Emily -- the velocity of time turns her voice into sugar water http://www.youtube.com/watch?v=jNA6zzoObxg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JGREWE <@t> OhioHealth.com Mon Oct 13 14:37:15 2008 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Mon Oct 13 14:37:23 2008 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 10/13/2008 and will not return until 10/16/2008. From plucas <@t> biopath.org Mon Oct 13 14:37:56 2008 From: plucas <@t> biopath.org (Paula Lucas) Date: Mon Oct 13 14:37:59 2008 Subject: [Histonet] Decolorize for IHC Message-ID: <20081013193756.2ECAB3424@centaur.cnchost.com> Hello, I need to decolorize an H&E stained section for Cytokeratin 20 and I would like to know the proper way of decolorizing for IHC. Is it even possible to get a CK 20 positive stain after doing so? Thanks, Paula Lucas HT Lab Manager Bio-Path Medical Group From LSebree <@t> uwhealth.org Mon Oct 13 14:47:10 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Mon Oct 13 14:47:14 2008 Subject: [Histonet] Decolorize for IHC In-Reply-To: <20081013193756.2ECAB3424@centaur.cnchost.com> Message-ID: We don't bother decolorizing H&Es since the IHC methodology generally takes out the histochemical staining....also, we're counterstaining with hematoxylin anyway. You should have no problem doing CK20 over an H&E. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Monday, October 13, 2008 2:38 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Decolorize for IHC Hello, I need to decolorize an H&E stained section for Cytokeratin 20 and I would like to know the proper way of decolorizing for IHC. Is it even possible to get a CK 20 positive stain after doing so? Thanks, Paula Lucas HT Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Mon Oct 13 14:54:22 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon Oct 13 14:54:30 2008 Subject: [Histonet] Decolorize for IHC In-Reply-To: Message-ID: I just did a CK20 on an H&E and Linda is correct, there is no need to decolorize before doing the CK20. The only thing I would suggest is that you are sure that all mounting media is removed after the coverslip comes off your H&E. The longer it's been coverslipped the more mindful of mounting media removal you should be. Good Luck, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sebree Linda A. Sent: Monday, October 13, 2008 2:47 PM To: Paula Lucas; histonet@pathology.swmed.edu Subject: RE: [Histonet] Decolorize for IHC We don't bother decolorizing H&Es since the IHC methodology generally takes out the histochemical staining....also, we're counterstaining with hematoxylin anyway. You should have no problem doing CK20 over an H&E. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Monday, October 13, 2008 2:38 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Decolorize for IHC Hello, I need to decolorize an H&E stained section for Cytokeratin 20 and I would like to know the proper way of decolorizing for IHC. Is it even possible to get a CK 20 positive stain after doing so? Thanks, Paula Lucas HT Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From candy.a.bales <@t> uth.tmc.edu Mon Oct 13 15:27:56 2008 From: candy.a.bales <@t> uth.tmc.edu (Bales, Candy A) Date: Mon Oct 13 15:27:59 2008 Subject: [Histonet] histology technician opening Message-ID: The University of Texas Dental Branch in Houston Texas has an opening for a Histotechnician I position for research and routine histology. No weekends, flex hours possible. For more details or to apply, please go to www.http://jobs.uth.tmc.edu/applicants/Central?quickFind=76346 . Thank you From candy.a.bales <@t> uth.tmc.edu Mon Oct 13 15:39:49 2008 From: candy.a.bales <@t> uth.tmc.edu (Bales, Candy A) Date: Mon Oct 13 15:39:52 2008 Subject: [Histonet] FW: histology technician opening Message-ID: Apologies for the incorrect website. It should be : http://jobs.uth.tmc.edu/applicants/Central?quickFind=76346 or http://jobs.uth.tmc.edu requisition # 093363. Thank you Candy Bales, HT From: Bales, Candy A Sent: Monday, October 13, 2008 3:28 PM To: 'histonet@lists.utsouthwestern.edu' Subject: histology technician opening The University of Texas Dental Branch in Houston Texas has an opening for a Histotechnician I position for research and routine histology. No weekends, flex hours possible. For more details or to apply, please go to www.http://jobs.uth.tmc.edu/applicants/Central?quickFind=76346 . Thank you From bruski33 <@t> aol.com Mon Oct 13 15:44:33 2008 From: bruski33 <@t> aol.com (bruski33@aol.com) Date: Mon Oct 13 15:45:09 2008 Subject: [Histonet] Re: histology ink washing off Message-ID: <8CAFB8BA21D3312-DAC-3E5@FWM-D11.sysops.aol.com> Blot off excess India ink. Paper towels. Then place the inked specimen?in a container?filled with?white vinegar. All it takes is a few seconds. Then?blot dry. We used to use Bouins but that stuff is nasty. Bruce Bouchard www.RIhistology.org From JEllin <@t> yumaregional.org Mon Oct 13 15:48:39 2008 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Mon Oct 13 15:48:49 2008 Subject: [Histonet] Histology Aid In-Reply-To: Message-ID: <29BE166A2CF48D459853F8EC57CD37E8011D8AC0@EXCHANGECLUSTER.yumaregional.local> Was wanting to get some feed back from everyone out there concerning the Histology Technical Aid,, How many are having their aid login/accession specimens? What is the error rate that you set for this type of work?, ie Spelling correct patient demographics,, ect. How many log in there specimens away from the grossing area and if so does your accessioner put in all patient information at that time?? What type of jobs does this person usually perform. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. From pruegg <@t> ihctech.net Mon Oct 13 15:44:28 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Oct 13 16:31:15 2008 Subject: [Histonet] IHC stainers Message-ID: <15F96CD97BAE4F209597B3DA6DCB117E@Patsyoffice> Folks, I have been asked to post this question about continuous feed batching for IHC stainers: "continuous feed" stainers - Bond is really the only one at 10 slides per batch, though the old Benchmark was similar at 20 per batch. The new Biocare is 12 per batch. We are wondering if people use the feature, and if so, how does it work for them to do many small batches during the day rather than one or two large batches. Does this really help workflow or is it just a marketing point. Thank you for your feed back, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From amosbrooks <@t> gmail.com Mon Oct 13 17:58:12 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Oct 13 17:58:17 2008 Subject: [Histonet] Expiration dates Message-ID: <582736990810131558y5e9e6190i433d461349c49570@mail.gmail.com> Amy, It is probably too late to suggest this, but I'd start by not buying such an instrument in the first place. Standardization is nice, but the tech should be smarter than the instrument. Many companies want techs running these instruments to be mindless drones that do little more than transport slides from microtome to stainer and back. Always look for an instrument that allows you maximum flexibility with every aspect of it's use. Other than that you would end up needing to "trick" the instrument into thinking that it is running something else that uses the same staining protocol. Just a shot in the dark, have you tried photocopying an active bar code and taping (gluing) it to the expired one? (Just make sure the assigned protocol matches in terms of retrieval technique and clonality for the detection) I'm sure that would violate the instrument manufacturer's terms of service, but it is clearly not functioning for you if you can't use it. Let me know how that works out for you, Amos Message: 4 Date: Mon, 13 Oct 2008 07:04:10 -0400 From: "Senn, Amy R" Subject: [Histonet] Expiration dates To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello all, My supervisor wanted me to ask everyone who's posting about the expiration dates of antibodies: How can you use expired antibodies on a stainer such as a Benchmark-which will not allow you to start the run due to expiration dates on the containers? Thanks!! Amy Senn Camp Hill, PA From Jackie.O'Connor <@t> abbott.com Tue Oct 14 02:18:01 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Oct 14 02:18:26 2008 Subject: [Histonet] Re: Modified Davidsons Vendor in Europe In-Reply-To: Message-ID: They are very competent - however, I am hoping to find a vendor in the interest of time management, as they must currently prepare huge volumes for literally hundreds of samples per week. It's all about lean laboratory practices - these are some of the finest histotechnologists in the world - I'm just trying to ease their burden. Jackie O' "Robert Richmond" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/13/2008 01:16 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Re: Modified Davidsons Vendor in Europe Jackie O'Connor wants to know if there is a vendor for modified Davidson's fixative in Europe. three parts tap water, three parts ethyl alcohol or reagent alcohol, two parts 37% formaldehyde, one part glacial acetic acid I would hope that the level of scientific education among European histologists is such that they would still be allowed to make this simple formulation themselves. Bob Richmond Samurai Pathologist Knoxville, Tennessee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Oct 14 08:15:31 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Oct 14 08:15:50 2008 Subject: [Histonet] Decolorize for IHC In-Reply-To: <20081013193756.2ECAB3424@centaur.cnchost.com> References: <20081013193756.2ECAB3424@centaur.cnchost.com> Message-ID: <48F463330200007700006279@gwmail4.harthosp.org> You don't need to decolorize. Remove the coverslip, rehydrate to water (the eosin will come out in the alcohol), and then begin your IHC procedure. Hopefully, the tissue is mounted on an adhesive or charged slide. If not, you may have to cut back your antigen retrieval. Also, you may want to take an unstained slide from your CK20 positive control tissue and stain it with H&E, and then run it in parallel. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Paula Lucas 10/13/08 3:37 PM >>> Hello, I need to decolorize an H&E stained section for Cytokeratin 20 and I would like to know the proper way of decolorizing for IHC. Is it even possible to get a CK 20 positive stain after doing so? Thanks, Paula Lucas HT Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Tue Oct 14 09:03:38 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Oct 14 09:03:46 2008 Subject: [Histonet] IHC stainers In-Reply-To: <15F96CD97BAE4F209597B3DA6DCB117E@Patsyoffice> Message-ID: We have found that when using the Bond, it is easier to get started with 10 slides, or 1 or 2 small cases. Our pathologists make requests throughout the day, and to have to hold up the entire run so that another case can be added creates a delay in results. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patsy Ruegg Sent: Monday, October 13, 2008 4:44 PM To: histonet@pathology.swmed.edu Subject: [Histonet] IHC stainers Folks, I have been asked to post this question about continuous feed batching for IHC stainers: "continuous feed" stainers - Bond is really the only one at 10 slides per batch, though the old Benchmark was similar at 20 per batch. The new Biocare is 12 per batch. We are wondering if people use the feature, and if so, how does it work for them to do many small batches during the day rather than one or two large batches. Does this really help workflow or is it just a marketing point. Thank you for your feed back, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From HornHV <@t> archildrens.org Tue Oct 14 09:17:55 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Oct 14 09:18:07 2008 Subject: [Histonet] Are there no LEAN labs out there? Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82E74@EMAIL.archildrens.org> I have not heard from any labs that have gone to the LEAN process. Are there none out there? I have heard from labs who are interested in it but not one response from a lab who is LEAN. Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From dellav <@t> musc.edu Tue Oct 14 10:09:58 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Oct 14 10:10:19 2008 Subject: [Histonet] RE: Are there no LEAN labs out there? In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82E74@EMAIL.archildrens.org> References: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82E74@EMAIL.archildrens.org> Message-ID: Hazel, I am scheduled to attend a Surgical Pathology Lean training workshop at Henry Ford Hospital in Detroit. You can see the information at this link http://www.henryford.com/body.cfm?id=50135 I don't know anyone who works in the lab there but you might find it useful to contact the histo lab by phone to have your questions addressed. Hope this helps. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, October 14, 2008 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are there no LEAN labs out there? I have not heard from any labs that have gone to the LEAN process. Are there none out there? I have heard from labs who are interested in it but not one response from a lab who is LEAN. Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Oct 14 11:06:46 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Oct 14 11:06:50 2008 Subject: [Histonet] CD105 and CD166 on human bone/bone marrow Message-ID: Hello All I need a bit of help here. We have been trying to localize CD105 and CD166 in human bone. Our control tissue is staining just fine (human tonsil and human skin) but we have been unable to demonstrate any of these cells within the bone marrow. Any help would be appreciated, especially if you have been able to get this to work on bone marrow samples. In the past we have been able to detect a very small number of CD166 positive cells in the bone marrow but recently we have not. The staining on our control sample has remained the same through out this process. thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 From liz <@t> premierlab.com Tue Oct 14 11:14:02 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Oct 14 11:14:06 2008 Subject: [Histonet] More bone questions Message-ID: I'm working with a client who it is growing cells on a demineralized bone matrix. We have processed the tissue both manually and on a VIP and we are seeing that the cells are not attached to the bone and that they are separated away from the bone. We are trying to figure out if this is a processing artifact and what we can do to prevent it. The cells appear to follow the structure of the bone its just that they are separated away from the bone and should be (in a perfect world) attached to or laying on top of the edge of the bone. If someone has worked with these types of samples before and has advice that would be great. thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 From SSCALISE <@t> beaumonthospitals.com Tue Oct 14 10:45:59 2008 From: SSCALISE <@t> beaumonthospitals.com (Sharon Scalise) Date: Tue Oct 14 11:17:31 2008 Subject: [Histonet] Are there no LEAN labs out there? In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82E74@EMAIL.archildrens.org> References: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82E74@EMAIL.archildrens.org> Message-ID: <48F48677.1CA9.00C8.0@beaumonthospitals.com> Here is a link to Henry Ford Hospital (Detroit, MI). They are a LEAN lab and they are holding a seminar for interested labs. Hope this helps. http://www.henryford.com/HFProductionSystem Sharon E. Scalise, HTL (ASCP) Histology Supervisor William Beaumont Hospital Royal Oak, MI 48073 248 898-5981 From gvdobbin <@t> ihis.org Tue Oct 14 11:26:05 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Tue Oct 14 11:26:48 2008 Subject: [Histonet] CD105 and CD166 on human bone/bone marrow Message-ID: Liz, If your controls are fine but your bone marrows are not, could it be the decalcification process that is damaging these antigens? Try using EDTA for decal instead of formic acid. Just a thought. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "And in the end it's not the years in your life that count. It's the life in your years." - Abraham Lincoln ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From kalschev <@t> svm.vetmed.wisc.edu Tue Oct 14 12:01:33 2008 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Tue Oct 14 12:02:03 2008 Subject: [Histonet] Liz - Cells on demineralized bone Message-ID: <001001c92e1e$82b3fc60$c5d76880@vetmed.wisc.edu> Liz, When I do some smaller odd type bone samples, in paraffin, we have found the cutting pulls the cells out of the nice, lacunae network and the cells sit on top. It is an artifact we have noted for years. Calcified sectioning might be better. Vicki From jflinn <@t> gmu.edu Tue Oct 14 12:33:48 2008 From: jflinn <@t> gmu.edu (Jane M Flinn) Date: Tue Oct 14 12:34:34 2008 Subject: [Histonet] Re: We are running a congo red stain and have siodium chloride cryatals precipa In-Reply-To: <48ED2820.3060801@ucsd.edu> References: <48ED2820.3060801@ucsd.edu> Message-ID: We are running a congo red stain and have sodium chloride crystals precipataing onto the slide. Does anyone know why and how we can fix this? jane "Life is short - make haste to be kind" Dr. Jane Flinn Director, Undergraduate Neuroscience Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: kbowden Date: Wednesday, October 8, 2008 5:37 pm Subject: Re: [Histonet] citrisolve? > I have used Citrisolve for serveral years. I don't think anything > compares to Xylene, but I do think this comes very close. I > change it > about once a week. It pretty much deparaffinizes in the same > amount of > time. Disposal is dependent on the regulations in your area. > When I > started using it our Environmental Health and Safety said pour it > down > the drain, but as time goes by and with more regulations now I > have to > collect it and have it removed with hazardous waste. > -- > Karen Bowden > Staff Research Associate II > University of CA, San Diego > Department of Orthopedics > 9500 Gilman Dr. 0630 > La Jolla, CA 92093-0630 > 858-534-4655 voice > 858-534-5304 fax > > > CONFIDENTIALITY NOTICE: > THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE > PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL > AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, > DISSEMINATION OR > OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION > BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS > PROHIBITED.IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT > THE SENDER AND > DELETE THE MATERIAL FROM ANY COMPUTER. > > > Patten, Nicole (NIH/NIAAA) [F] wrote: > > Does anyone have any experience using Citrisolve as a Xylene > alternative> to deparaffinize tissue sections? Do I use it just > like Xylene for the > > same amount of time? Can it be reused or should I use new Citrisolve > > each time? How do I dispose of it? > > > > > > > > Any help at all would be much appreciated. Thanks! > > > > > > > > Nicole J. Patten > > Post-Baccalaureate Fellow/IRTA > > National Institutes of Health > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kmilne <@t> bccancer.bc.ca Tue Oct 14 12:45:00 2008 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Tue Oct 14 12:45:19 2008 Subject: [Histonet] Expiration dates In-Reply-To: References: Message-ID: <07979E76B0869D4E8C9FE4AA9FC065780466955C@srvex03.phsabc.ehcnet.ca> Hi Amy, I use the Ventana Discovery and I add my antibodies manually as I'm in research and don't feel like shelling out extra for empty containers to put my Abs in. Do you have an option on the Benchmark to switch the primary Ab step to "titration" or something like that (can't remember what the exact step name is but not the autodispense one). I don't even know if that's an option on the Benchmark but with the Discovery, I can just open that machine and add it straight at that step. Perhaps you could get your antibody out of the dispensor and try it that way. Katy Milne Deeley Research Centre - Message: 4 Date: Mon, 13 Oct 2008 07:04:10 -0400 From: "Senn, Amy R" Subject: [Histonet] Expiration dates To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello all, My supervisor wanted me to ask everyone who's posting about the expiration dates of antibodies: How can you use expired antibodies on a stainer such as a Benchmark-which will not allow you to start the run due to expiration dates on the containers? Thanks!! Amy Senn Camp Hill, PA From renafail <@t> bellsouth.net Tue Oct 14 12:54:07 2008 From: renafail <@t> bellsouth.net (renafail@bellsouth.net) Date: Tue Oct 14 12:54:20 2008 Subject: [Histonet] Re: We are running a congo red stain and have siodium chloride cryatals precipa In-Reply-To: References: <48ED2820.3060801@ucsd.edu> Message-ID: <101420081754.26787.48F4DCBF00038F87000068A322230650629B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net> Are you filtering the solutions before immersing the slides? The sodium chloride is a saturated solution. Rena Fail -------------- Original message from Jane M Flinn : -------------- We are running a congo red stain and have sodium chloride crystals precipataing > onto the slide. Does anyone know why and how we can fix this? jane > > "Life is short - make haste to be kind" > > Dr. Jane Flinn > Director, Undergraduate Neuroscience Program > George Mason University, 3F5 > 4400 University Dr. > Fairfax, VA 22030 > Phone: 703-993-4107 > Fax: 703-993-1359 > > > ----- Original Message ----- > From: kbowden > Date: Wednesday, October 8, 2008 5:37 pm > Subject: Re: [Histonet] citrisolve? > > > I have used Citrisolve for serveral years. I don't think anything > > compares to Xylene, but I do think this comes very close. I > > change it > > about once a week. It pretty much deparaffinizes in the same > > amount of > > time. Disposal is dependent on the regulations in your area. > > When I > > started using it our Environmental Health and Safety said pour it > > down > > the drain, but as time goes by and with more regulations now I > > have to > > collect it and have it removed with hazardous waste. > > -- > > Karen Bowden > > Staff Research Associate II > > University of CA, San Diego > > Department of Orthopedics > > 9500 Gilman Dr. 0630 > > La Jolla, CA 92093-0630 > > 858-534-4655 voice > > 858-534-5304 fax > > > > > > CONFIDENTIALITY NOTICE: > > THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE > > PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL > > AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, > > DISSEMINATION OR > > OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION > > BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS > > PROHIBITED.IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT > > THE SENDER AND > > DELETE THE MATERIAL FROM ANY COMPUTER. > > > > > > Patten, Nicole (NIH/NIAAA) [F] wrote: > > > Does anyone have any experience using Citrisolve as a Xylene > > alternative> to deparaffinize tissue sections? Do I use it just > > like Xylene for the > > > same amount of time? Can it be reused or should I use new Citrisolve > > > each time? How do I dispose of it? > > > > > > > > > > > > Any help at all would be much appreciated. Thanks! > > > > > > > > > > > > Nicole J. Patten > > > Post-Baccalaureate Fellow/IRTA > > > National Institutes of Health > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jflinn <@t> gmu.edu Tue Oct 14 13:18:43 2008 From: jflinn <@t> gmu.edu (Jane M Flinn) Date: Tue Oct 14 13:18:55 2008 Subject: [Histonet] Re: We are running a congo red stain and have siodium chloride cryatals precipa In-Reply-To: References: <48ED2820.3060801@ucsd.edu> Message-ID: Problem solved. jane "Life is short - make haste to be kind" Dr. Jane Flinn Director, Undergraduate Neuroscience Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: Jane M Flinn Date: Tuesday, October 14, 2008 1:33 pm Subject: [Histonet] Re: We are running a congo red stain and have siodium chloride cryatals precipa > We are running a congo red stain and have sodium chloride crystals > precipataing onto the slide. Does anyone know why and how we can > fix this? jane > > "Life is short - make haste to be kind" > > Dr. Jane Flinn > Director, Undergraduate Neuroscience Program > George Mason University, 3F5 > 4400 University Dr. > Fairfax, VA 22030 > Phone: 703-993-4107 > Fax: 703-993-1359 > > > ----- Original Message ----- > From: kbowden > Date: Wednesday, October 8, 2008 5:37 pm > Subject: Re: [Histonet] citrisolve? > > > I have used Citrisolve for serveral years. I don't think > anything > > compares to Xylene, but I do think this comes very close. I > > change it > > about once a week. It pretty much deparaffinizes in the same > > amount of > > time. Disposal is dependent on the regulations in your area. > > When I > > started using it our Environmental Health and Safety said pour > it > > down > > the drain, but as time goes by and with more regulations now I > > have to > > collect it and have it removed with hazardous waste. > > -- > > Karen Bowden > > Staff Research Associate II > > University of CA, San Diego > > Department of Orthopedics > > 9500 Gilman Dr. 0630 > > La Jolla, CA 92093-0630 > > 858-534-4655 voice > > 858-534-5304 fax > > > > > > CONFIDENTIALITY NOTICE: > > THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE > > PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN > CONFIDENTIAL> AND/OR PRIVILEGED MATERIAL. ANY REVIEW, > RETRANSMISSION, > > DISSEMINATION OR > > OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS > INFORMATION> BY PERSONS OR ENTITIES OTHER THAN THE INTENDED > RECIPIENT IS > > PROHIBITED.IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT > > THE SENDER AND > > DELETE THE MATERIAL FROM ANY COMPUTER. > > > > > > Patten, Nicole (NIH/NIAAA) [F] wrote: > > > Does anyone have any experience using Citrisolve as a Xylene > > alternative> to deparaffinize tissue sections? Do I use it just > > like Xylene for the > > > same amount of time? Can it be reused or should I use new > Citrisolve> > each time? How do I dispose of it? > > > > > > > > > > > > Any help at all would be much appreciated. Thanks! > > > > > > > > > > > > Nicole J. Patten > > > Post-Baccalaureate Fellow/IRTA > > > National Institutes of Health > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -------------- next part -------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jflinn <@t> gmu.edu Tue Oct 14 13:20:23 2008 From: jflinn <@t> gmu.edu (Jane M Flinn) Date: Tue Oct 14 13:20:31 2008 Subject: [Histonet] Re: We are running a congo red stain and have siodium chloride cryatals precipa In-Reply-To: <101420081754.26787.48F4DCBF00038F87000068A322230650629B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net> References: <48ED2820.3060801@ucsd.edu> <101420081754.26787.48F4DCBF00038F87000068A322230650629B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net> Message-ID: Thanks, the student indeed had a super saturated solution, Jane "Life is short - make haste to be kind" Dr. Jane Flinn Director, Undergraduate Neuroscience Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: renafail@bellsouth.net Date: Tuesday, October 14, 2008 1:54 pm Subject: Re: [Histonet] Re: We are running a congo red stain and have siodium chloride cryatals precipa > > Are you filtering the solutions before immersing the slides? The > sodium chloride is a saturated solution. > Rena Fail > -------------- Original message from Jane M Flinn > : -------------- > > We are running a congo red stain and have sodium chloride crystals > precipataing > > onto the slide. Does anyone know why and how we can fix this? jane > > > > "Life is short - make haste to be kind" > > > > Dr. Jane Flinn > > Director, Undergraduate Neuroscience Program > > George Mason University, 3F5 > > 4400 University Dr. > > Fairfax, VA 22030 > > Phone: 703-993-4107 > > Fax: 703-993-1359 > > > > > > ----- Original Message ----- > > From: kbowden > > Date: Wednesday, October 8, 2008 5:37 pm > > Subject: Re: [Histonet] citrisolve? > > > > > I have used Citrisolve for serveral years. I don't think > anything > > > compares to Xylene, but I do think this comes very close. I > > > change it > > > about once a week. It pretty much deparaffinizes in the same > > > amount of > > > time. Disposal is dependent on the regulations in your area. > > > When I > > > started using it our Environmental Health and Safety said pour > it > > > down > > > the drain, but as time goes by and with more regulations now I > > > have to > > > collect it and have it removed with hazardous waste. > > > -- > > > Karen Bowden > > > Staff Research Associate II > > > University of CA, San Diego > > > Department of Orthopedics > > > 9500 Gilman Dr. 0630 > > > La Jolla, CA 92093-0630 > > > 858-534-4655 voice > > > 858-534-5304 fax > > > > > > > > > CONFIDENTIALITY NOTICE: > > > THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY > FOR THE > > > PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN > CONFIDENTIAL> > AND/OR PRIVILEGED MATERIAL. ANY REVIEW, > RETRANSMISSION, > > > DISSEMINATION OR > > > OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS > INFORMATION> > BY PERSONS OR ENTITIES OTHER THAN THE INTENDED > RECIPIENT IS > > > PROHIBITED.IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE > CONTACT > > > THE SENDER AND > > > DELETE THE MATERIAL FROM ANY COMPUTER. > > > > > > > > > Patten, Nicole (NIH/NIAAA) [F] wrote: > > > > Does anyone have any experience using Citrisolve as a Xylene > > > alternative> to deparaffinize tissue sections? Do I use it > just > > > like Xylene for the > > > > same amount of time? Can it be reused or should I use new > Citrisolve> > > each time? How do I dispose of it? > > > > > > > > > > > > > > > > Any help at all would be much appreciated. Thanks! > > > > > > > > > > > > > > > > Nicole J. Patten > > > > Post-Baccalaureate Fellow/IRTA > > > > National Institutes of Health > > > > > > > > > > > > > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From jflinn <@t> gmu.edu Tue Oct 14 13:28:46 2008 From: jflinn <@t> gmu.edu (Jane M Flinn) Date: Tue Oct 14 13:28:50 2008 Subject: [Histonet] Re: We are running a congo red stain and have siodium chloride cryatals precipa In-Reply-To: References: <48ED2820.3060801@ucsd.edu> Message-ID: Thanks for the helpful comments. jane "Life is short - make haste to be kind" Dr. Jane Flinn Director, Undergraduate Neuroscience Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: Jane M Flinn Date: Tuesday, October 14, 2008 2:18 pm Subject: Re: [Histonet] Re: We are running a congo red stain and have siodium chloride cryatals precipa > Problem solved. jane > > "Life is short - make haste to be kind" > > Dr. Jane Flinn > Director, Undergraduate Neuroscience Program > George Mason University, 3F5 > 4400 University Dr. > Fairfax, VA 22030 > Phone: 703-993-4107 > Fax: 703-993-1359 > > > ----- Original Message ----- > From: Jane M Flinn > Date: Tuesday, October 14, 2008 1:33 pm > Subject: [Histonet] Re: We are running a congo red stain and have > siodium chloride cryatals precipa > > > We are running a congo red stain and have sodium chloride > crystals > > precipataing onto the slide. Does anyone know why and how we can > > fix this? jane > > > > "Life is short - make haste to be kind" > > > > Dr. Jane Flinn > > Director, Undergraduate Neuroscience Program > > George Mason University, 3F5 > > 4400 University Dr. > > Fairfax, VA 22030 > > Phone: 703-993-4107 > > Fax: 703-993-1359 > > > > > > ----- Original Message ----- > > From: kbowden > > Date: Wednesday, October 8, 2008 5:37 pm > > Subject: Re: [Histonet] citrisolve? > > > > > I have used Citrisolve for serveral years. I don't think > > anything > > > compares to Xylene, but I do think this comes very close. I > > > change it > > > about once a week. It pretty much deparaffinizes in the same > > > amount of > > > time. Disposal is dependent on the regulations in your area. > > > When I > > > started using it our Environmental Health and Safety said pour > > it > > > down > > > the drain, but as time goes by and with more regulations now I > > > have to > > > collect it and have it removed with hazardous waste. > > > -- > > > Karen Bowden > > > Staff Research Associate II > > > University of CA, San Diego > > > Department of Orthopedics > > > 9500 Gilman Dr. 0630 > > > La Jolla, CA 92093-0630 > > > 858-534-4655 voice > > > 858-534-5304 fax > > > > > > > > > CONFIDENTIALITY NOTICE: > > > THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY > FOR THE > > > PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN > > CONFIDENTIAL> AND/OR PRIVILEGED MATERIAL. ANY REVIEW, > > RETRANSMISSION, > > > DISSEMINATION OR > > > OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS > > INFORMATION> BY PERSONS OR ENTITIES OTHER THAN THE INTENDED > > RECIPIENT IS > > > PROHIBITED.IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE > CONTACT > > > THE SENDER AND > > > DELETE THE MATERIAL FROM ANY COMPUTER. > > > > > > > > > Patten, Nicole (NIH/NIAAA) [F] wrote: > > > > Does anyone have any experience using Citrisolve as a Xylene > > > alternative> to deparaffinize tissue sections? Do I use it > just > > > like Xylene for the > > > > same amount of time? Can it be reused or should I use new > > Citrisolve> > each time? How do I dispose of it? > > > > > > > > > > > > > > > > Any help at all would be much appreciated. Thanks! > > > > > > > > > > > > > > > > Nicole J. Patten > > > > Post-Baccalaureate Fellow/IRTA > > > > National Institutes of Health > > > > > > > > > > > > > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -------------- next part -------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Tue Oct 14 13:37:02 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Oct 14 13:37:08 2008 Subject: [Histonet] Expiration dates In-Reply-To: <07979E76B0869D4E8C9FE4AA9FC065780466955C@srvex03.phsabc.ehcnet.ca> Message-ID: Yes, you can always run in "manual" mode....editing the protocol to "titration" rather than "antibody". That does indeed allow you to manually apply the antibody when the instrument signals its time to do so. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Milne, Katy Sent: Tuesday, October 14, 2008 12:45 PM To: arsenn@hsh.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Expiration dates Hi Amy, I use the Ventana Discovery and I add my antibodies manually as I'm in research and don't feel like shelling out extra for empty containers to put my Abs in. Do you have an option on the Benchmark to switch the primary Ab step to "titration" or something like that (can't remember what the exact step name is but not the autodispense one). I don't even know if that's an option on the Benchmark but with the Discovery, I can just open that machine and add it straight at that step. Perhaps you could get your antibody out of the dispensor and try it that way. Katy Milne Deeley Research Centre - Message: 4 Date: Mon, 13 Oct 2008 07:04:10 -0400 From: "Senn, Amy R" Subject: [Histonet] Expiration dates To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello all, My supervisor wanted me to ask everyone who's posting about the expiration dates of antibodies: How can you use expired antibodies on a stainer such as a Benchmark-which will not allow you to start the run due to expiration dates on the containers? Thanks!! Amy Senn Camp Hill, PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Tue Oct 14 13:49:42 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Oct 14 13:49:47 2008 Subject: [Histonet] Expiration dates In-Reply-To: References: <07979E76B0869D4E8C9FE4AA9FC065780466955C@srvex03.phsabc.ehcnet.ca> Message-ID: <5b6eb13e0810141149n63bd855fh808b3b6ffe73b87b@mail.gmail.com> True, but unless you have every slide on the run as titration the machine wont start. That's a good trick if you just have a titration run. Mark On Tue, Oct 14, 2008 at 11:37 AM, Sebree Linda A. wrote: > Yes, you can always run in "manual" mode....editing the protocol to > "titration" rather than "antibody". That does indeed allow you to > manually apply the antibody when the instrument signals its time to do > so. > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > DB1-223 VAH > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Milne, > Katy > Sent: Tuesday, October 14, 2008 12:45 PM > To: arsenn@hsh.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Expiration dates > > Hi Amy, > > I use the Ventana Discovery and I add my antibodies manually as I'm in > research and don't feel like shelling out extra for empty containers to > put my Abs in. Do you have an option on the Benchmark to switch the > primary Ab step to "titration" or something like that (can't remember > what the exact step name is but not the autodispense one). I don't even > know if that's an option on the Benchmark but with the Discovery, I can > just open that machine and add it straight at that step. Perhaps you > could get your antibody out of the dispensor and try it that way. > > Katy Milne > Deeley Research Centre > > - > Message: 4 > Date: Mon, 13 Oct 2008 07:04:10 -0400 > From: "Senn, Amy R" > Subject: [Histonet] Expiration dates > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > > > My supervisor wanted me to ask everyone who's posting about the > expiration dates of antibodies: How can you use expired antibodies on a > stainer such as a Benchmark-which will not allow you to start the run > due to expiration dates on the containers? > > > > > > Thanks!! > > > > Amy Senn > > Camp Hill, PA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MSHERWOOD <@t> PARTNERS.ORG Tue Oct 14 14:28:27 2008 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Oct 14 14:28:32 2008 Subject: [Histonet] Re: Manual for Tissue-TEC 5 EM A-1 5101 Embedding Center Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9701A7C876@PHSXMB30.partners.org> To all: Would anyone have a manual for the above embedding center? Or could tell us how to set the temperature on the unit? Our settings got eliminated and we can't figure out how to re-set them? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From Jacqueline.Farnsworth <@t> cls.ab.ca Tue Oct 14 14:51:31 2008 From: Jacqueline.Farnsworth <@t> cls.ab.ca (Jacqueline.Farnsworth@cls.ab.ca) Date: Tue Oct 14 14:51:49 2008 Subject: [Histonet] RE: Are there no LEAN labs out there? In-Reply-To: Message-ID: <1E832A8226461B4B83A586FF052EBB373E30B3@Mail3.calgary.com> HI Hazel Our lab has, in fact, been "LEANED". We were somewhat limited by budget of course, but we did a fair amount of streamlining. We are at 5 sites in the city, so each site presented it's own challenges. We are currently in the process of maintaining our processes. If you would like to e mail me off - line, we can 'chat' some more. Thanks Jacquie jacqueline.farnsworth@cls.ab.ca Jacqueline Farnsworth Anatomic Pathology, Tech III Calgary Laboratory Services Calgary, Alberta, Canada -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Della Speranza, Vinnie Sent: October 14, 2008 9:10 AM To: 'Horn, Hazel V'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Are there no LEAN labs out there? Hazel, I am scheduled to attend a Surgical Pathology Lean training workshop at Henry Ford Hospital in Detroit. You can see the information at this link http://www.henryford.com/body.cfm?id=50135 I don't know anyone who works in the lab there but you might find it useful to contact the histo lab by phone to have your questions addressed. Hope this helps. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, October 14, 2008 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are there no LEAN labs out there? I have not heard from any labs that have gone to the LEAN process. Are there none out there? I have heard from labs who are interested in it but not one response from a lab who is LEAN. Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From gagnone <@t> KGH.KARI.NET Tue Oct 14 14:53:14 2008 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Tue Oct 14 14:53:24 2008 Subject: [Histonet] Titration Runs on Ventana Ultra Message-ID: The new Ventana Ultra allows slides requiring titration/manual application of antibody to be loaded concurrently with protocols using dispensers. This is because it is a continuous access system, any slide/any time. This is an exciting change for those of us who have had to run titrations as separate runs on BenchMark XT. Hi Brenda. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From Ronald.Houston <@t> nationwidechildrens.org Tue Oct 14 15:21:36 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Oct 14 15:22:04 2008 Subject: [Histonet] Titration Runs on Ventana Ultra In-Reply-To: Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20FEC24B3@chi2k3ms01.columbuschildrens.net> And how much does this baby cost? Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Tuesday, October 14, 2008 3:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Titration Runs on Ventana Ultra The new Ventana Ultra allows slides requiring titration/manual application of antibody to be loaded concurrently with protocols using dispensers. This is because it is a continuous access system, any slide/any time. This is an exciting change for those of us who have had to run titrations as separate runs on BenchMark XT. Hi Brenda. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From nfournier <@t> sasktel.net Tue Oct 14 16:19:14 2008 From: nfournier <@t> sasktel.net (N Fournier) Date: Tue Oct 14 16:19:20 2008 Subject: [Histonet] cryostat blues Message-ID: <1e5b2c7c15545.48f4b872@sasktel.net> Hi Everyone: I am encountering problems with curling whenever I lift the antiroll plate and attempt to retrieve the section. Often the top portion of the section will remain flat while the bottom curls. I am sectioning fixed rat brains on a Vibratome Ultrapro 5000 cyrostat. The brains were placed in cryomolds and embedded in OCT, and stored at -80 degree C before sectioning. The specimen isallowed to equilbrated in the chamber for 45 min before sectioning commences. The knife is new, and the specimen temperature is approx -13 to -15 degree, and chamber temperature at -20 degree C. The blade angle is currently set to around 2 to 3 degrees (as recommended by the manufacturer's instructions), however, this has been modified several times and the problem occurs at other angles. Does anyone have some suggestions of what could be causing this and most importantly what I could do to make it stop. Thanks, Neil From CIngles <@t> uwhealth.org Tue Oct 14 16:19:33 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Oct 14 16:20:36 2008 Subject: [Histonet] Titration Runs on Ventana Ultra References: <979FF5962E234F45B06CF0DB7C1AABB20FEC24B3@chi2k3ms01.columbuschildrens.net> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120183@uwhis-xchng3.uwhis.hosp.wisc.edu> Probably about the same as a human one... :) Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Houston, Ronald Sent: Tue 10/14/2008 3:21 PM To: Gagnon, Eric; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Titration Runs on Ventana Ultra And how much does this baby cost? Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 From MSHERWOOD <@t> PARTNERS.ORG Tue Oct 14 16:31:47 2008 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Tue Oct 14 16:31:50 2008 Subject: [Histonet] Re: Manual for Tissue-TEC 5 EM A-1 5101 Embedding Center In-Reply-To: <00b801c92e43$2ea52070$8bef6150$@com> References: <073AE2BEA1C2BA4A8837AB6C4B943D9701A7C87A@PHSXMB30.partners.org> <00b801c92e43$2ea52070$8bef6150$@com> Message-ID: <073AE2BEA1C2BA4A8837AB6C4B943D9701A7C881@PHSXMB30.partners.org> Thank you Charles! I contacted Sakura and someone called me immediately. You were right; the light had been turned down! It's hard when you don't have a manual. For future reference, I was told that you have to buy the manual (@ $125)! -----Original Message----- From: Charles E. Brown, Jr. [mailto:ccebjr@embarqmail.com] Sent: Tuesday, October 14, 2008 5:24 PM To: Sherwood, Margaret Subject: RE: [Histonet] Re: Manual for Tissue-TEC 5 EM A-1 5101 Embedding Center Have you turned the wheel, below the screen, to bring-up the screen? The beeping indicates activity and generally, that the action is not accepted. Charles PS: I don't know why but your response was returned with an incorrect e-mail. The correct e-mail is ccebjr@embarqmail.com -----Original Message----- From: Sherwood, Margaret [mailto:MSHERWOOD@PARTNERS.ORG] Sent: Tuesday, October 14, 2008 4:13 PM To: ccebjr@embarqmail.com Subject: FW: [Histonet] Re: Manual for Tissue-TEC 5 EM A-1 5101 Embedding Center -----Original Message----- From: Sherwood, Margaret Sent: Tuesday, October 14, 2008 4:10 PM To: 'cbjr@embarqmail.comc' Subject: RE: [Histonet] Re: Manual for Tissue-TEC 5 EM A-1 5101 Embedding Center Someone sent us the instructions via email and another person is faxing us a copy of the manual. We did try following the directions, but we are getting no readout on the screen. I'm wondering if the LED has burned out?! I even tried switching the console on/off to no avail. When I press the arrow keys (or any of the keys!), I hear beeping, but nothing appears on the screen. Peggy -----Original Message----- From: Charles E. Brown, Jr. [mailto:ccebjr@embarqmail.com] Sent: Tuesday, October 14, 2008 4:01 PM To: Sherwood, Margaret Subject: RE: [Histonet] Re: Manual for Tissue-TEC 5 EM A-1 5101 Embedding Center Dear Peggy, I have a manual but do not have access to it as I am currently working as a contract traveler. I am familiar with the instrument and would be willing to walk you through the process. My current contract has me working here in Boston. If you are interested, please let me know and we can proceed from there. Charles E. Brown, Jr. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Tuesday, October 14, 2008 3:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Manual for Tissue-TEC 5 EM A-1 5101 Embedding Center To all: Would anyone have a manual for the above embedding center? Or could tell us how to set the temperature on the unit? Our settings got eliminated and we can't figure out how to re-set them? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherwood@partners.org The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From guerzong <@t> yahoo.com Tue Oct 14 16:54:46 2008 From: guerzong <@t> yahoo.com (Godfrey Guerzon) Date: Tue Oct 14 16:57:18 2008 Subject: [Histonet] Nails Message-ID: <165896.90716.qm@web59501.mail.ac4.yahoo.com> We are having problems getting nail sections to stick to the slide.? They almost always fall off the slide during H&E?staining.? We?use plus slides and various adhesives -?sta-on, hairspray, etc. with no success. Is there anybody that can help me by sharing their secret method to get nail sections survibe H&E staining without falling off? Please help!!!! Thanks. Godfrey From Charlene.Henry <@t> STJUDE.ORG Tue Oct 14 17:09:05 2008 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Tue Oct 14 17:09:09 2008 Subject: [Histonet] Trichrome Stain Message-ID: <03E1F5968F60C5448635D49D38B283ED0275A0E44E@SJMEMXMBS11.stjude.sjcrh.local> We are going to discontinue the use of the Ventana Trichrome kit because the kit expires so quickly and we do not perform enough Trichrome stains to use all of the reagents. We have not performed a manual Trichrome stain in such a long time that I was wondering what Trichrome protocol is most labs performing now? Thanks, Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 From jnocito <@t> satx.rr.com Tue Oct 14 17:29:17 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Oct 14 17:29:20 2008 Subject: [Histonet] Nails References: <165896.90716.qm@web59501.mail.ac4.yahoo.com> Message-ID: <636DE2E8C0AE44258579044B2E06A693@yourxhtr8hvc4p> it's a good thing you asked. I let my nails (well not my nails personally, but the specimen nails) soak in 20% ammonium hydroxide or 20% sodium hydroxide for at least 1 hour (sodium hydroxide works better, but whatever you can get). Then I rinse the blocks in running water for 2-3 minutes, then place in formalin for normal processing. When cutting, I use positive slides with a waterbath filled with distilled water (if you use an adhesive, you will be defeating the purpose of the charged slides) cut at 4 microns and place in an 80 C oven for 15 minutes. Make sure that the slides are completely dried before beginning staining (if the slides are still a little wet, I put them in front of a small fan to blow off all the water). My lab performs at least 10 blocks per day and we don't have problems with the H&E or the PAS/fungus. Hey, that's why they call me Joe, the Toe. JTT ----- Original Message ----- From: "Godfrey Guerzon" To: Sent: Tuesday, October 14, 2008 4:54 PM Subject: [Histonet] Nails We are having problems getting nail sections to stick to the slide. They almost always fall off the slide during H&E staining. We use plus slides and various adhesives - sta-on, hairspray, etc. with no success. Is there anybody that can help me by sharing their secret method to get nail sections survibe H&E staining without falling off? Please help!!!! Thanks. Godfrey _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Oct 14 18:09:43 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Oct 14 18:14:15 2008 Subject: [Histonet] Trichrome Stain References: <03E1F5968F60C5448635D49D38B283ED0275A0E44E@SJMEMXMBS11.stjude.sjcrh.local> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A73DF6FD@LTA3VS011.ees.hhs.gov> Prior to purchasing the Artisan we were using the Gomori's One-Step trichrome with great success. Kit H017 at Polyscientific...www.polyrnd.com ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Henry, Charlene Sent: Tue 10/14/2008 6:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trichrome Stain We are going to discontinue the use of the Ventana Trichrome kit because the kit expires so quickly and we do not perform enough Trichrome stains to use all of the reagents. We have not performed a manual Trichrome stain in such a long time that I was wondering what Trichrome protocol is most labs performing now? Thanks, Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jwatson <@t> gnf.org Tue Oct 14 19:43:29 2008 From: jwatson <@t> gnf.org (James Watson) Date: Tue Oct 14 19:43:35 2008 Subject: [Histonet] RE: [IHCRG] IHC antibody expiration question for CAP. . In-Reply-To: <48EF9A710200007700006112@gwmail4.harthosp.org> References: <48efa647.8702be0a.491d.6a4cSMTPIN_ADDED@mx.google.com> <48EF9A710200007700006112@gwmail4.harthosp.org> Message-ID: I do not know much about why expiration dates were started, but I would assume that fear of being sued has a lot to do with it. A missed diagnosis and a lawyer could go after the lab, pathologist, doctor, and antibody manufacturer. A manufacturer would loose a lot more money in a lawsuit than they would make by short dating expiration dates on an antibody. This idea of a "stability guarantee date" might relieve the manufacturer of responsibility and place it on the technician, pathologist and lab.* James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org *Disclaimer: This post comes from someone in research that has no stake in this other than the being concerned about the high cost of healthcare and a proper diagnosis. -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Richard Cartun Sent: Friday, October 10, 2008 3:10 PM To: Jackie.O'Connor@abbott.com; pruegg@ihctech.net; Histonet Cc: 'ihcrg Group (E-mail)'; ancillarypath@mac.com; charlene.henry@stjude.org Subject: [IHCRG] IHC antibody expiration question for CAP. . I have antibodies that work great 10-20 years after their expiration date. Once again, I propose that the manufactures eliminate the "expiration date" and use a "stability guarantee date". If the antibody is used after that date then the user would be responsible for validating its performance. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Jackie M O'Connor 10/10/08 3:12 PM >>> When I was making and testing antibodies for a manufacturer, we had retest dates on all the lots. As each lot reached or was past it's expiration date, we would test it against a fresh antibody lot - if it still met the exact same standard, we would extend the expiration date 3 or 6 months. Most antibodies stored at 40C I tested were still good 6 months past expiration. ALL antibodies were still good 30 days past expiration, regardless of the storage conditions. There was a strict rule about thawing frozen aliquots - no more than 3x freeze-thaw cycles, as this did damage the antibody. Throwing away frozen aliquots because they have expired is nuts. If your results are still meeting standards, it's a waste. Jackie O' "Patsy Ruegg" Sent by: ihcrg@googlegroups.com 10/10/2008 02:00 PM Please respond to pruegg@ihctech.net To , , "'ihcrg Group \(E-mail\)'" cc Subject [IHCRG] Re: IHC antibody expiration question for CAP. . Charlene, This is new to me as well. Hadi can you give us the rule where it says you can use expired abs if you freeze aliquots before they expire? Folks, do not throw away expired antibodies. The IHCRG has a reagent bank, you can send them to me. We will even provide you with a fedex act. No to use to ship the reagents. I have a refrigerator full of these reagents labs being CAP inspected have sent me. We use them in research and for teaching purposes. If any of you members of the IHCRG want to check something out we have in the bank, perhaps before you purchase it for a research project, you can do that. Just send me a message asking if I have what you need. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Henry, Charlene Sent: Friday, October 10, 2008 12:48 PM To: 'ancillarypath@mac.com'; ihcrg Group (E-mail) Subject: [IHCRG] Re: IHC antibody expiration question for CAP. . What do you do when the antibody data sheet states "do not freeze" and if freezing the antibody is accepted by CAP wouldn't the CAP Checklist state that this practice is acceptable? Also I have been to a couple CAP workshops and this question was ask. The CAP instructors stated that the antibody cannot be used past the expiration date regardless if it is frozen or stored at -20?C. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Hadi Yaziji Sent: Friday, October 10, 2008 1:28 PM To: ihcrg Group (E-mail) Subject: [IHCRG] Re: IHC antibody expiration question for CAP. . You need to educate the inspectors that it's accepted by the CAP. Most inspectors don't even know about this, because it hardly ever comes up. ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Oct 10, 2008, at 1:55 PM, Mark Tarango wrote: I'm a little skeptical too, but if this isn't allowed then it should be. On Fri, Oct 10, 2008 at 9:22 AM, Sebree Linda A. wrote: Call me skeptical but I've never heard of CAP accepting this method of shelf-life extension. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of ancillarypath@mac.com Sent: Friday, October 10, 2008 11:19 AM To: ihcrg Group (E-mail) Subject: [IHCRG] Re: IHC antibody expiration question for CAP There is a CAP-accepted way of working around this... If you aliquot the antibody before its expiration date and store it in -20 freezer, the clock is in effect "frozen" too. For example, if there are 6 months left before the antibody expires and you freeze the aliquots for 5 years, then if you thaw one of the frozen aliquots, the thawed aliquot will still be good for 6 months. Just make sure you don't thaw and re-freeze the aliquot. Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Oct 10, 2008, at 12:15 PM, Swain, Frances L wrote: Hey everyone. I use outdated antibodies I work in a research service laboratory. The reason CAP has these rules is because of patient protection. If you use an outdated antibody and the specimen comes back negative and later it was discovered that the patient had the problem that the antibody was suppose to identify. Maybe the patient dies or is permanently ill for the rest of their lives. Can you live with that? CAP is suppose to make sure that all of the labs (clinical only right now) are working under the same guidelines. They are not making these rules to make you spend more money but to protect the patient and make sure the diagnosis is as correct as can be. I agree that it is unfair to destroy antibodies that are used only once or twice before they expire. Maybe the companies preparing these antibodies should extend their expiration times? Just thought I would put my two cents worth in. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Mark Tarango Sent: Friday, October 10, 2008 11:10 AM To: themagoos@rushmore.com Cc: ihcrg@googlegroups.com Subject: [IHCRG] Re: IHC antibody expiration question for CAP The rules are the rules. We need to change them back. Mark On Fri, Oct 10, 2008 at 8:41 AM, Jason and Heather wrote: CAP question ANP.22432 states: Are all immunohistochemical reagents used within their indicated expiration dates? I am wondering what labs across the country are doing to answer this question. IHC antibodies are very expensive and can be validated on every run that is made. Some of these concentrated antibodies are diluted out so far that only a very small portion of them are used before they expire. Are some labs still using outdated antibodies? If so how do you get away with it when you are inspected by CAP? Or are you paying the high prices to throw out the majority of your antibodies so you can answer this question yes? I thank you in advance to your response. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgough@clinlab.com Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. 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aol.com Tue Oct 14 20:57:39 2008 From: jmyers1 <@t> aol.com (jmyers1@aol.com) Date: Tue Oct 14 20:58:09 2008 Subject: [Histonet] restarting DAB reaction Message-ID: <8CAFC8089BEB42D-C04-85D@WEBMAIL-MA20.sysops.aol.com> Emily: Although it might seem that the reapplication of a substrate-chromogen solution?after removal of the coverslip/mounting media and rehydration?should work, it?very rarely does.? The scientific reason is that, after the specimen has been dehydrated and coverslipped,?the enzyme label (e.g. polymer-HRP) applied in the original staining procedure is 'dead' -- meaning that it lacks the?power to drive another chromogen-polymerization reaction.? Your best bet is start over, 'from scratch'... Good Luck, J.D.?Myers, M.S., CT(ASCP) *************************************** Message: 1 Date: Mon, 13 Oct 2008 13:17:49 -0400 From: "Emily Sours" Subject: [Histonet] restarting DAB reaction To: histonet@lists.utsouthwestern.edu Has anyone ever tried to continue a DAB reaction after the slides have been washed and coverslipped? I can't think of a reason why this wouldn't work, since we just wash the slides in PBS and water after the DAB step. We use a vectastain kit for this--would we need to add more of the ABC reagent (after removing the coverslips and washing in PBS) and then do DAB? Emily From AnthonyH <@t> chw.edu.au Tue Oct 14 22:36:11 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Oct 14 22:36:26 2008 Subject: [Histonet] restarting DAB reaction In-Reply-To: <8CAFC8089BEB42D-C04-85D@WEBMAIL-MA20.sysops.aol.com> Message-ID: Yes, I would also agree that this would probably be the case. I have not tried to redo the DAB step after counterstaining, dehydrating, clearing and mounting. I would expect it not to work. Though I could easily be corrected. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jmyers1@aol.com Sent: Wednesday, 15 October 2008 12:58 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] restarting DAB reaction Emily: Although it might seem that the reapplication of a substrate-chromogen solution?after removal of the coverslip/mounting media and rehydration?should work, it?very rarely does.? The scientific reason is that, after the specimen has been dehydrated and coverslipped,?the enzyme label (e.g. polymer-HRP) applied in the original staining procedure is 'dead' -- meaning that it lacks the?power to drive another chromogen-polymerization reaction.? Your best bet is start over, 'from scratch'... Good Luck, J.D.?Myers, M.S., CT(ASCP) *************************************** Message: 1 Date: Mon, 13 Oct 2008 13:17:49 -0400 From: "Emily Sours" Subject: [Histonet] restarting DAB reaction To: histonet@lists.utsouthwestern.edu Has anyone ever tried to continue a DAB reaction after the slides have been washed and coverslipped? I can't think of a reason why this wouldn't work, since we just wash the slides in PBS and water after the DAB step. We use a vectastain kit for this--would we need to add more of the ABC reagent (after removing the coverslips and washing in PBS) and then do DAB? Emily _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From pieronelva01 <@t> bigpond.com Wed Oct 15 02:27:09 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Wed Oct 15 02:27:23 2008 Subject: [Histonet] Expiration dates References: <07979E76B0869D4E8C9FE4AA9FC065780466955C@srvex03.phsabc.ehcnet.ca> Message-ID: <67ABA71D5CBD46AFB723A0CD5D909CCF@pentium4> Hi Amy I use VMS Benchmark XT and when the antibody has expired I "create" a new lot number by putting (EXP1) at the end of it and adding one year to the expiration date. THen add the antibody dilution to the prepkit using the "Refill" option rather than the "Partial" option. As long as you're using appropriate controls with your staining runs, there is no problem extending the life of the antibody. Regards Piero Nelva Anatomical pathology Monash Medical Centre Victoria Australia Hello all, My supervisor wanted me to ask everyone who's posting about the expiration dates of antibodies: How can you use expired antibodies on a stainer such as a Benchmark-which will not allow you to start the run due to expiration dates on the containers? Thanks!! Amy Senn Camp Hill, PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.8.0/1725 - Release Date: 10/14/2008 9:25 PM From pieronelva01 <@t> bigpond.com Wed Oct 15 02:31:26 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Wed Oct 15 02:31:31 2008 Subject: [Histonet] Titration Runs on Ventana Ultra References: Message-ID: I'm also looking forward to the Australian Release of the VMS Ultra for that very reason (due here late 2009). We currently wait until there is a "free" day to do our titrations. They are few and far between!! Regards Piero Nelva Anatomical Pathology Monash Medical Centre Victoria Australia ----- Original Message ----- From: "Gagnon, Eric" To: Sent: Wednesday, October 15, 2008 6:53 AM Subject: [Histonet] Titration Runs on Ventana Ultra The new Ventana Ultra allows slides requiring titration/manual application of antibody to be loaded concurrently with protocols using dispensers. This is because it is a continuous access system, any slide/any time. This is an exciting change for those of us who have had to run titrations as separate runs on BenchMark XT. Hi Brenda. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.8.0/1725 - Release Date: 10/14/2008 9:25 PM From louise.renton <@t> gmail.com Wed Oct 15 03:03:43 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Oct 15 03:03:47 2008 Subject: [Histonet] Tunnel vs TUNEL Message-ID: hey guys, what is the correct way of writing this? I always thought it was TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling ), but I recently saw an article in a reputable journal refer to "tunnel" satining - is ths something differnt or just sloppiness? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From lpwenk <@t> sbcglobal.net Wed Oct 15 03:59:21 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Oct 15 03:59:41 2008 Subject: [Histonet] Expiration dates In-Reply-To: <67ABA71D5CBD46AFB723A0CD5D909CCF@pentium4> Message-ID: <000301c92ea4$511673f0$0202a8c0@HPPav2> Except here in the US, where we have federal laws that prohibit using expired reagents. Enforced by laboratory accrediting agencies. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospitals Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piero Nelva Sent: Wednesday, October 15, 2008 3:27 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Expiration dates Hi Amy I use VMS Benchmark XT and when the antibody has expired I "create" a new lot number by putting (EXP1) at the end of it and adding one year to the expiration date. THen add the antibody dilution to the prepkit using the "Refill" option rather than the "Partial" option. As long as you're using appropriate controls with your staining runs, there is no problem extending the life of the antibody. Regards Piero Nelva Anatomical pathology Monash Medical Centre Victoria Australia Hello all, My supervisor wanted me to ask everyone who's posting about the expiration dates of antibodies: How can you use expired antibodies on a stainer such as a Benchmark-which will not allow you to start the run due to expiration dates on the containers? Thanks!! Amy Senn Camp Hill, PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- ---- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.8.0/1725 - Release Date: 10/14/2008 9:25 PM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pieronelva01 <@t> bigpond.com Wed Oct 15 05:36:31 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Wed Oct 15 05:36:39 2008 Subject: [Histonet] Expiration dates References: <000301c92ea4$511673f0$0202a8c0@HPPav2> Message-ID: <8E7CD251965847DA9B26CC815C9BA458@pentium4> Ouch!! Piero ----- Original Message ----- From: "Lee & Peggy Wenk" To: "'Piero Nelva'" ; Sent: Wednesday, October 15, 2008 7:59 PM Subject: RE: [Histonet] Expiration dates > Except here in the US, where we have federal laws that prohibit using > expired reagents. Enforced by laboratory accrediting agencies. > > Peggy A. Wenk, HTL(ASCP)SLS > Beaumont Hospitals > Royal Oak, MI 48073 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piero > Nelva > Sent: Wednesday, October 15, 2008 3:27 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Expiration dates > > Hi Amy > > I use VMS Benchmark XT and when the antibody has expired I "create" a new > lot number by putting (EXP1) at the end of it and adding one year to the > expiration date. THen add the antibody dilution to the prepkit using the > "Refill" option rather than the "Partial" option. As long as you're using > appropriate controls with your staining runs, there is no problem > extending > the life of the antibody. > > Regards > > Piero Nelva > Anatomical pathology > Monash Medical Centre > Victoria > Australia > > > > > > Hello all, > > > > My supervisor wanted me to ask everyone who's posting about the expiration > dates of antibodies: How can you use expired antibodies on a stainer such > as a Benchmark-which will not allow you to start the run due to expiration > dates on the containers? > > > > > > Thanks!! > > > > Amy Senn > > Camp Hill, PA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ---------------------------------------------------------------------------- > ---- > > > > No virus found in this incoming message. > Checked by AVG - http://www.avg.com > Version: 8.0.173 / Virus Database: 270.8.0/1725 - Release Date: 10/14/2008 > 9:25 PM > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.8.0/1725 - Release Date: 10/14/2008 9:25 PM From talulahgosh <@t> gmail.com Wed Oct 15 07:54:49 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Oct 15 07:54:56 2008 Subject: [Histonet] cryostat blues In-Reply-To: <1e5b2c7c15545.48f4b872@sasktel.net> References: <1e5b2c7c15545.48f4b872@sasktel.net> Message-ID: Unfortunately, this sounds like static electricity. Leica suggests spraying anti-static spray in the chamber (!). You could also ground the metal in the chamber using metal wire touching something metal outside the chamber (the Russian post-doc solution, very clever). We keep 100% EtOH in a small container (about 100 ml) in the back of the chamber to reduce static, which I learned from a long ago histonet post, so I have no idea why it works. Otherwise, buy an anti-static brush, which I hear exists, but have never seen. Emily -- the velocity of time turns her voice into sugar water http://www.youtube.com/watch?v=jNA6zzoObxg From JWeems <@t> sjha.org Wed Oct 15 08:28:52 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Oct 15 08:29:13 2008 Subject: [Histonet] cryostat blues In-Reply-To: References: <1e5b2c7c15545.48f4b872@sasktel.net> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA509F49D@ITSSSXM01V6.one.ads.che.org> Those who are full of hot air can also blow gently and will generate enough humidity to reduce the static. J:>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Wednesday, October 15, 2008 8:55 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryostat blues Unfortunately, this sounds like static electricity. Leica suggests spraying anti-static spray in the chamber (!). You could also ground the metal in the chamber using metal wire touching something metal outside the chamber (the Russian post-doc solution, very clever). We keep 100% EtOH in a small container (about 100 ml) in the back of the chamber to reduce static, which I learned from a long ago histonet post, so I have no idea why it works. Otherwise, buy an anti-static brush, which I hear exists, but have never seen. Emily -- the velocity of time turns her voice into sugar water http://www.youtube.com/watch?v=jNA6zzoObxg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From kimtournear <@t> yahoo.com Wed Oct 15 08:33:16 2008 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Wed Oct 15 08:33:22 2008 Subject: [Histonet] Job Message-ID: <314915.12642.qm@web54201.mail.re2.yahoo.com> Hi, If anyone in Tucson Arizona is looking for a parttime histotech/mohs tech, I know someone who is interested...preferrably afternoons and/or saturdays...You can reply back at this address... Thanks, Kim Tournear, HT (ASCP), AIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ? ? ~Don't?let your life end before it begins~ ? OU Rocks!!!! From LSebree <@t> uwhealth.org Wed Oct 15 08:41:07 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Wed Oct 15 08:41:16 2008 Subject: [Histonet] FW: CAP Inquiry regarding expired Abs Message-ID: My supervisor had our QA person check into whether CAP had changed their stance on using expired Abs or more exactly, on whether it was ok to extend the expiration date if aliquots were frozen. Below is the response she received. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: Joan Rose (s) [mailto:jrose@cap.org] Sent: Tuesday, October 14, 2008 4:32 PM To: Haack Lori A. Subject: CAP Inquiry Dear Ms. Haack, I have received your CAP inquiry regarding the use of reagents beyond the manufacturer stated expiration date. The Federal Rules and Regulations and the College of American Pathologists' Laboratory Accreditation Program prohibit the use of expired materials for patient testing. As delineated in multiple CAP Checklists, reagents must not be used beyond their stated or assigned expiration date. The only exception is for rare Blood Bank antibodies as stated in the note of the Transfusion Medicine (TRM) checklist item TRM.31250: Rare reagents (e.g., anti-Jk(a), anti-Le(a), etc.) may be used beyond their expiration date if appropriate positive and negative controls are run and react as expected. This exception is permitted by the FDA. This does not apply to reagents that are readily available. Please refer to the Federal Register 1992(Feb 28): 7164[42 CFR 493.1205(e)(1)] for more information. You can access the federal regulations at the web-site http://www.hcfa.gov/medicaid/clia/cliahome.htm. I hope this information is helpful. If you have any additional questions, I can be reached at the phone number or e-mail address listed below. Thank you for your participation in the Laboratory Accreditation Program. Sincerely, Joan Rose, MT(ASCP)SH Technical Specialist Laboratory Accreditation Program College of American Pathologists 1-800-323-4040 extension 7257 jrose@cap.org From Jan.Minshew <@t> leica-microsystems.com Wed Oct 15 09:26:45 2008 From: Jan.Minshew <@t> leica-microsystems.com (Jan.Minshew@leica-microsystems.com) Date: Wed Oct 15 09:27:11 2008 Subject: [Histonet] cryostat blues In-Reply-To: Message-ID: Hello Emily, I'm so sorry if someone told you to spray an anti-static spray into the cryostat chamber. That is not a recommended practice and it should not be done. Spraying anything into a cryochamber creates an aerosol that can contaminate the air in the breathing zone of the operator...and we all know what is lurking in the depths of our cryostats. As you suggested, storing a small container of alcohol in the back of the cryostat while sectioning (remove when finished) and grounding the blade holder are effective and safe ways to reduce static electricity. You can also try placing an anti-static clothes dryer sheet in front of or behind the knife holder. Again, I apologize for the misinformation. Please feel free to contact me if I can help in any other way. Best wishes, Jan Minshew, HT/HTL(ASCP) Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 800.248.0123 Toll Free 847.405.7051 Direct 847.405.6560 Fax www.leica-microsystems.com Click Here for this month's special offers! "Emily Sours" To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject Re: [Histonet] cryostat blues 10/15/2008 07:54 AM Unfortunately, this sounds like static electricity. Leica suggests spraying anti-static spray in the chamber (!). You could also ground the metal in the chamber using metal wire touching something metal outside the chamber (the Russian post-doc solution, very clever). We keep 100% EtOH in a small container (about 100 ml) in the back of the chamber to reduce static, which I learned from a long ago histonet post, so I have no idea why it works. Otherwise, buy an anti-static brush, which I hear exists, but have never seen. Emily -- the velocity of time turns her voice into sugar water http://www.youtube.com/watch?v=jNA6zzoObxg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Michael.Campisano <@t> PERKINELMER.COM Wed Oct 15 09:03:27 2008 From: Michael.Campisano <@t> PERKINELMER.COM (Campisano,Michael) Date: Wed Oct 15 10:25:40 2008 Subject: [Histonet] histology and cytology marketing survey Message-ID: <6D3EB9350BD3EE4698E5CFED2BA094C403B52545@AMERMAIL02.PERKINELMER.NET> Hello everyone, You are invited to participate in a market survey on signal amplification in histology and cytology from PerkinElmer. You're input would be very much appreciated, and would help us improve our offering in the future. The online survey is available at this web page. http://www.surveymonkey.com/s.aspx?sm=ujhPmw47QoyBM41yWpuB8w_3d_3d All the best, Michael Campisano Associate Product Leader | Research Reagents PerkinElmer, Inc. | Bio-discovery From jcline <@t> wchsys.org Wed Oct 15 12:28:54 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Wed Oct 15 12:29:02 2008 Subject: [Histonet] Open position Message-ID: I have a FTE Histotechnician job open. M-F varying hours, plus no weekends, no call time no autopsies Contact HR at 301-665-4500, Mike Talhelm or go online to wchsys.org and go to Human Resources. We do about 17,000 cases a year, about 85,000 slides a year. 330 IHC per month (Benchmark XT), 200 specials a month (NeXES & by hand) Each tech has a different bench assignment each week. We are an hour from Baltimore, Md and Wash. D.C. A brand new hospital is to be finished by 2010. The lab is in another building that will be attached to the new hospital. We are located in Hagerstown, Md. The area is very attractive and full of things to do. (History buffs, outdoor activities) Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From mrsseagle <@t> yahoo.com Wed Oct 15 12:57:15 2008 From: mrsseagle <@t> yahoo.com (MICHELLE SEAGLE) Date: Wed Oct 15 12:57:19 2008 Subject: [Histonet] Excelsior ES Tissue Processor?? Message-ID: <191568.57058.qm@web51805.mail.re2.yahoo.com> Does anyone currently have the Excelsior ES Tissue Processor, and if so are you experiencing problems with this equipment?? What do you think about this processor?? Michelle Seagle HT (ASCP) ? From laurie.colbert <@t> huntingtonhospital.com Wed Oct 15 13:56:21 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Oct 15 13:56:35 2008 Subject: [Histonet] IHC for Amyloid L Message-ID: <57BE698966D5C54EAE8612E8941D768303F54927@EXCHANGE3.huntingtonhospital.com> Does anyone know of a reference lab that does an IHC stain for Amyloid L (amyloid light chain)? I can find labs that do Amyloid A and Amyloid P, but not Amyloid L. Laurie Colbert Huntington Hospital Pasadena, CA From Terri.Brown <@t> Northside.com Wed Oct 15 14:32:30 2008 From: Terri.Brown <@t> Northside.com (Terri Brown) Date: Wed Oct 15 14:30:52 2008 Subject: [Histonet] cryostat blues In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA509F49D@ITSSSXM01V6.one.ads.che.org> References: <1e5b2c7c15545.48f4b872@sasktel.net> <5D64396A0D4A5346BEBC759022AAEAA509F49D@ITSSSXM01V6.one.ads.che.org> Message-ID: <8CEB6DA1A3F35743800669D4CFE21F7D0689F54B@NSMXMS04.northside.local> Good going!!!! Only us OLD TECH know this. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, October 15, 2008 9:29 AM To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat blues Those who are full of hot air can also blow gently and will generate enough humidity to reduce the static. J:>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Wednesday, October 15, 2008 8:55 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] cryostat blues Unfortunately, this sounds like static electricity. Leica suggests spraying anti-static spray in the chamber (!). You could also ground the metal in the chamber using metal wire touching something metal outside the chamber (the Russian post-doc solution, very clever). We keep 100% EtOH in a small container (about 100 ml) in the back of the chamber to reduce static, which I learned from a long ago histonet post, so I have no idea why it works. Otherwise, buy an anti-static brush, which I hear exists, but have never seen. Emily -- the velocity of time turns her voice into sugar water http://www.youtube.com/watch?v=jNA6zzoObxg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From AnthonyH <@t> chw.edu.au Wed Oct 15 16:52:38 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Oct 15 16:52:54 2008 Subject: [Histonet] restarting DAB reaction In-Reply-To: Message-ID: If we don't give it a go, how would we know? Now I know!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Jacqui Detmar [mailto:detmar@lunenfeld.ca] Sent: Thursday, 16 October 2008 12:52 AM To: Tony Henwood; jmyers1@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] restarting DAB reaction Hey there. Actually, I recently tried to re-do a DAB reaction on two slides (mouse placental sections) that I had cover-slipped about a year ago, thinking it would work. Nothing happened. Even the RBCs just sat there and laughed at my clumsy attempt. I slunk away in shame. Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 25 Orde Street, room 6-1001 AJ Toronto, ON, Canada M5T 3H7 Tel: 416-586-4800 x5607 Fax: 416-586-8588 email: detmar@lunenfeld.ca ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tony Henwood Sent: Tue 10/14/2008 11:36 PM To: jmyers1@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] restarting DAB reaction Yes, I would also agree that this would probably be the case. I have not tried to redo the DAB step after counterstaining, dehydrating, clearing and mounting. I would expect it not to work. Though I could easily be corrected. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jmyers1@aol.com Sent: Wednesday, 15 October 2008 12:58 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] restarting DAB reaction Emily: Although it might seem that the reapplication of a substrate-chromogen solution?after removal of the coverslip/mounting media and rehydration?should work, it?very rarely does.? The scientific reason is that, after the specimen has been dehydrated and coverslipped,?the enzyme label (e.g. polymer-HRP) applied in the original staining procedure is 'dead' -- meaning that it lacks the?power to drive another chromogen-polymerization reaction.? Your best bet is start over, 'from scratch'... Good Luck, J.D.?Myers, M.S., CT(ASCP) *************************************** Message: 1 Date: Mon, 13 Oct 2008 13:17:49 -0400 From: "Emily Sours" Subject: [Histonet] restarting DAB reaction To: histonet@lists.utsouthwestern.edu Has anyone ever tried to continue a DAB reaction after the slides have been washed and coverslipped? I can't think of a reason why this wouldn't work, since we just wash the slides in PBS and water after the DAB step. We use a vectastain kit for this--would we need to add more of the ABC reagent (after removing the coverslips and washing in PBS) and then do DAB? Emily _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From meghak <@t> CLEMSON.EDU Wed Oct 15 18:08:37 2008 From: meghak <@t> CLEMSON.EDU (Megha Kumar) Date: Wed Oct 15 18:08:42 2008 Subject: [Histonet] (no subject) Message-ID: <4538.130.127.57.6.1224112117.squirrel@wm.clemson.edu> hey does anyone have the manual for cryostat - Microm HN 505??? mk Ms. Megha Kumar Doctoral student , Chapman Lab Dept. of Biological Sciences 132 Long Hall Clemson University Clemson SC 29634 864-656-3384 From mickie25 <@t> netzero.net Wed Oct 15 19:01:54 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Oct 15 19:01:55 2008 Subject: [Histonet] (no subject) In-Reply-To: <4538.130.127.57.6.1224112117.squirrel@wm.clemson.edu> References: <4538.130.127.57.6.1224112117.squirrel@wm.clemson.edu> Message-ID: Call Belair Instrument Company in NJ. They should have a copy of one. 800-783-9424. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Megha Kumar Sent: Wednesday, October 15, 2008 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) hey does anyone have the manual for cryostat - Microm HN 505??? mk Ms. Megha Kumar Doctoral student , Chapman Lab Dept. of Biological Sciences 132 Long Hall Clemson University Clemson SC 29634 864-656-3384 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.8.0/1724 - Release Date: 10/14/2008 2:02 AM From RSRICHMOND <@t> aol.com Wed Oct 15 20:58:48 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Oct 15 20:58:53 2008 Subject: [Histonet] Re: histology ink washing off In-Reply-To: <48F5E26C.7020101@comcast.net> References: <48F24571.2030109@comcast.net> <48F5E26C.7020101@comcast.net> Message-ID: Mark Ray certainly raises some interesting points about the interplay of power in a pathology service. If as a pathologist I ask Purchasing to get me an item, they'll delight in ignoring me. Rather than deal with that, I'll go to a craft store or a Wal-Mart or whatever - probably combining it with another visit to the store - and save the confrontation. I think I saw some of Kurosawa's films half a century ago, but these days I never go to films, only to movies. Bob Richmond Samurai Pathologist Knoxville TN *************************************************************** On Wed, Oct 15, 2008 at 8:30 AM, Mark Ray wrote: > I don't disagree with you, Bob, but a lot of people don't have the good > fortune or fortitude to get around as much as you must have to. I remember > when I worked in the lab, it always seemed an imposition to have to go > shopping for something needed for work. You might work overtime, week > after week, and the the weekend would be the supermarket, some other chores > and housekeeping. Craft and art supplies, they don't have that at the 7/11. > It was always easier to put it on a PO, when possible, and have it > delivered direct. While you're going from employer to employer, righting > wrongs, rescuing distressed women and eviscerating the bad guys, it's > probably not very inconvenient to run some errands on the way. Not quite > the same as it is for us rice paddy peasants, we got enough trouble fending > off the bandits and politicians. Anyway, have you seen Kurosawa's "Red > Beard?" Mifune plays a doctor in a 19th Century clinic. Not samurai > action, but at one point he has a great ju jitsu scene. > > Robert Richmond wrote: >> >> Hi Mark Ray! >> >> For a four dollar item that needs replacing less than once a year, I'd >> rather just pay for it out of my own pocket than go to the trouble of >> begging to borrow a catalog and begging purchasing to order the item >> and waiting a week or two for it. That's what I do for small tools >> like strainers, hacksaws, pliers and what have you also. >> >> Bob Richmond >> >> On Sun, Oct 12, 2008 at 2:44 PM, Mark Ray wrote: >> >>> >>> Most institutions have a discount contract with a large office supply >>> company. Borrow a catalog from the office staff. That's usually the >>> cheapest deal on india ink and you don't have to go to an outside vendor >>> that your purchasing department never heard of. >>> >>> Robert Richmond wrote: >>> >>>> >>>> Inks - both ordinary india ink and colored inks - used for marking >>>> histology specimens will usually stay on quite satisfactorily if you >>>> make sure to blot the specimen dry before inking. I never anything to >>>> fix the ink to the specimen. 2% to 5% acetic acid is simple to use if >>>> you or your pathologist want it. Don't use acetone (flammable) or >>>> Bouin's fixative (toxic and messy). >>>> >>>> Ink doesn't adhere well to cauterized tissue surfaces. It adheres >>>> reasonably well to cauterized breast tissue, not at all to the >>>> cauterized surfaces of LEEP specimens of the cervix (where the >>>> cauterized surfaces themselves are an adequate guide to the margins >>>> for the microscopist). >>>> >>>> Ordinary india ink - most of what the pathologist uses - is easily >>>> bought at artist's or craft supply stores, much cheaper and in more >>>> convenient containers than you get from medical supply houses. When >>>> multiple colors are needed, some people use tattoo inks (cheap, and >>>> available in numerous colors), but most use inks made specially for >>>> this purpose. I prefer the Davidson marking inks (now available from >>>> ordinary lab vendors like whatever Thermo and Cardinal are called this >>>> week. I have no commercial connection to this product.) >>>> >>>> Inks dry out and go bad if the containers aren't promptly capped after >>>> use. Pathologists tend to forget this, and the histotech who assists >>>> the pathologist should make sure that the caps are replaced. >>>> >>>> The little dye capsules are an abomination - they squirt and flood the >>>> specimen and your clothes with unwanted dye. >>>> >>>> Bob Richmond >>>> Samurai Pathologist >>>> Knoxville TN From louise.renton <@t> gmail.com Thu Oct 16 02:13:19 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Thu Oct 16 02:13:22 2008 Subject: [Histonet] Tunnel vs TUNEL In-Reply-To: References: Message-ID: Thanks to those who answered, those who were serious and those who weren't :-) Now i can sleep easier, nights. Anyway its all the fault of spellcheckers (which is satining got in..to those who picked it up!) love u all On 10/15/08, Jacqui Detmar wrote: > > Hey there. I'm in the cell death field and do *plenty* of TUNELs. The > short form you are using is correct. I have also seen it as "terminal > deoxynucleotidyl transferase-mediated dUTP nick-end labeling". Anyway, > Tunnel is dead wrong....someone just made a mistake. I am shocked to learn > that even reputable journals are prone to these . > > Jacqui > > > > Jacqui Detmar, Post-doctoral Fellow > Samuel Lunenfeld Research Institute, > Mount Sinai Hospital, > 25 Orde Street, room 6-1001 AJ > Toronto, ON, Canada > M5T 3H7 > > Tel: 416-586-4800 x5607 > Fax: 416-586-8588 > email: detmar@lunenfeld.ca > > > ------------------------------ > *From:* histonet-bounces@lists.utsouthwestern.edu on behalf of louise > renton > *Sent:* Wed 10/15/2008 4:03 AM > *To:* Histonet@lists.utsouthwestern.edu > *Subject:* [Histonet] Tunnel vs TUNEL > > > > hey guys, > > what is the correct way of writing this? I always thought it was TUNEL > (Terminal deoxynucleotidyl transferase dUTP nick end labeling ), but I > recently saw an article in a reputable journal refer to "tunnel" satining - > is ths something differnt or just sloppiness? > > > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From leiker <@t> buffalo.edu Thu Oct 16 08:54:20 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Thu Oct 16 08:54:29 2008 Subject: [Histonet] Manual Microwave Method of Tissue Processing In-Reply-To: <483C4EA5F30EA4D1BB8620B6@bchwxp2702.ad.med.buffalo.edu> References: <100920082016.13660.48EE66B5000B54A40000355C22230647629B0A02D208 9B9A019C04040A0DBFCE9C07089B0E03030C@att.net> <9783C1674804287BC7B3809E@bchwxp2702.ad.med.buffalo.edu> <483C4EA5F30EA4D1BB8620B6@bchwxp2702.ad.med.buffalo.edu> Message-ID: <55A4C1F60D15C1C29B5122A1@bchwxp2702.ad.med.buffalo.edu> Thanks to everyone who replied to my query! I received many insightful responses. Merced --On Thursday, October 09, 2008 5:19 PM -0400 Merced Leiker wrote: > What are people's exerience with manual processing of FFPE tissues using > a microwave? My boss has me looking into a manual method of processing > tissues to cut costs - small lab with small budget getting smaller all > the time... > > Merced M Leiker > Research Technician II > 354 BRB (Lee Lab) / 140 Farber Hall (mail) > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > In order to put yourself in someone else's shoes, > you must first take off yours. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 In order to put yourself in someone else's shoes, you must first take off yours. From mhamilton <@t> GENVEC.com Thu Oct 16 09:09:57 2008 From: mhamilton <@t> GENVEC.com (Hamilton, Melissa) Date: Thu Oct 16 09:10:42 2008 Subject: [Histonet] LacZ Staining of Tumors Message-ID: <71CB4E18A03CB4448F2DD236C6C7C316017F0D12@genexch.genvec.com> I am looking for a protocol to fix and stain in paraffin embedded subcutaneously grown mouse tumors for LacZ. Can anyone help? M. From michelle.steinkrauss <@t> novartis.com Thu Oct 16 12:37:49 2008 From: michelle.steinkrauss <@t> novartis.com (michelle.steinkrauss@novartis.com) Date: Thu Oct 16 12:37:57 2008 Subject: [Histonet] Michelle Steinkrauss is out of the office. Message-ID: I will be out of the office starting 10/16/2008 and will not return until 10/20/2008. If you require immediate assistance, please contact Michelle Broome at x 47477. From TJJ <@t> Stowers-Institute.org Thu Oct 16 13:02:53 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Oct 16 13:03:20 2008 Subject: [Histonet] Re: LacZ Staining of Tumors Message-ID: Melissa wrote: >>I am looking for a protocol to fix and stain in paraffin embedded subcutaneously grown mouse tumors for LacZ. Can anyone help? M.<< Far as I know, one cannot stain paraffin embedded material with x-gal. We will do either whole mount X-gal staining, post-fix, and then paraffin process and section, or we will fix, sucrose cryoprotect, freeze and then cryosection to do the X-gal staining. Send me an email if you want to have our protocols for doing either. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From CHRISH <@t> HEALTHCARESCOUTS.COM Thu Oct 16 13:39:24 2008 From: CHRISH <@t> HEALTHCARESCOUTS.COM (Chris Handrahan) Date: Thu Oct 16 13:46:47 2008 Subject: [Histonet] ASCP certified Histotechs Needed LARGE referral bonuses Message-ID: For full time permanent positions Seattle, WA Pittsburgh, PA Dallas,TX Fort Worth, TX Clearwater, FL San Antonio, TX Richmond, VA St Petersburg, FL Milwaukee, WI Overland Park, KS Plattsburgh, NY Charlotte, NC Myrtle Beach, SC With employment opportunities all over the US, we are always looking for qualified and motivated professionals. Recommend a friend or family member, and you could be eligible for up to $1,000 through Healthcare Scouts' referral program. Call us today to learn more! Chris Handrahan Managing Director of Allied Health Healthcare Scouts 800-708-0605 office 321-231-5427 cell chrish@healthcarescouts.com www.healthcarescouts.com From mike <@t> pathview.com Thu Oct 16 14:01:00 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Oct 16 14:01:37 2008 Subject: [Histonet] Are there no LEAN labs out there? In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82E74@EMAIL.archildrens.org> References: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82E74@EMAIL.archildrens.org> Message-ID: <007401c92fc1$958a9250$c09fb6f0$@com> Hazel, we are an information system vendor who has built their entire computer system around LEAN. If you'd care to hear any details please email me outside the group. In a nutshell, you never have to enter a case # in the system. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, October 14, 2008 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are there no LEAN labs out there? I have not heard from any labs that have gone to the LEAN process. Are there none out there? I have heard from labs who are interested in it but not one response from a lab who is LEAN. Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdboydhisto <@t> yahoo.com Thu Oct 16 14:36:09 2008 From: kdboydhisto <@t> yahoo.com (KELLY BOYD) Date: Thu Oct 16 14:36:14 2008 Subject: [Histonet] HSV AND HPV I AND II Message-ID: <849304.98448.qm@web58606.mail.re3.yahoo.com> Does anyone know if there is an IHC?vendor who makes?a HPV antibody or a?HSV antibody?for In Vitro Diagnostic use? From TJJ <@t> Stowers-Institute.org Thu Oct 16 14:45:12 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Oct 16 14:45:35 2008 Subject: [Histonet] Re: LacZ Staining of Tumors In-Reply-To: <040a01c92fc6$471ad260$96893cd1@DJ4VDH31> Message-ID: Markus, we have successfully stained with x-gal, then decalcified with formic acid (ImmunoCal) and then paraffin processed. In our hands, if we did the x-gal technique first and then decalcified with EDTA, the blue positive staining came out. It does not using formic acid. I have seen published reports successfully x-gal staining by decalcifying with EDTA first, then staining with x-gal and paraffin processing. Beware doing this technique on bone though. Osteoclasts in normal bone will stain positively with the X-gal staining procedure (ref. Histol Histopathol (2007) 22: 971-976 B-Galactosidase staining on bone marrow. The osteoclast pitfall) Best wishes, Teri -----Original Message----- From: Markus F. Meyenhofer [mailto:micro@superlink.net] Sent: Thursday, October 16, 2008 2:35 PM To: Johnson, Teri Subject: Re: [Histonet] Re: LacZ Staining of Tumors Thank you for this note. I want to do this on bone (Paraffin). Do you think whole mounts on bone will work, plus de-cal? Regards, Markus ----- Original Message ----- From: "Johnson, Teri" To: Sent: Thursday, October 16, 2008 2:02 PM Subject: [Histonet] Re: LacZ Staining of Tumors Melissa wrote: >>I am looking for a protocol to fix and stain in paraffin embedded >>subcutaneously grown mouse tumors for LacZ. Can anyone help? M.<< Far as I know, one cannot stain paraffin embedded material with x-gal. We will do either whole mount X-gal staining, post-fix, and then paraffin process and section, or we will fix, sucrose cryoprotect, freeze and then cryosection to do the X-gal staining. Send me an email if you want to have our protocols for doing either. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Thu Oct 16 15:02:18 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Thu Oct 16 15:02:24 2008 Subject: [Histonet] X-Gal stain of bone In-Reply-To: References: Message-ID: Dear Markus, We do X-Gal staining on bone and in the best scenario the animals are perfused with glutaraldehyde (GA). PFA tends to quench the beta-gal activity and the results may not be 100% accurate (underestimation of positivity). Though if you want to do IHC on these samples, I am sure you know that the GA fix obliterates that possibility. Also it is critical to decalcify in EDTA rather than an acid decal. Let me know if you need more details, Andrea At 2:45 PM -0500 10/16/08, Johnson, Teri wrote: > >-----Original Message----- >From: Markus F. Meyenhofer [mailto:micro@superlink.net] >Sent: Thursday, October 16, 2008 2:35 PM >To: Johnson, Teri >Subject: Re: [Histonet] Re: LacZ Staining of Tumors > > >Thank you for this note. >I want to do this on bone (Paraffin). >Do you think whole mounts on bone will work, plus de-cal? Regards, Markus -- From victor <@t> pathology.washington.edu Thu Oct 16 15:12:17 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Oct 16 15:12:23 2008 Subject: [Histonet] PSLIM Update Message-ID: <48F7A021.1030602@pathology.washington.edu> Our PSLIM died before it ever made it to production testing. Our developer finally got some nice looking slides to print with a 2D bar code. Then for some unknown reason it jammed and had to be returned. They are sending a new unit with updated software. It has potential but can't be jamming after a few slides. I'll let you know when we take the new unit live into the lab. Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From Alistair.Lindsay <@t> btinternet.com Thu Oct 16 15:36:24 2008 From: Alistair.Lindsay <@t> btinternet.com (Alistair Lindsay) Date: Thu Oct 16 15:39:39 2008 Subject: [Histonet] Pig endothelium....again Message-ID: Dear Histonetters, You may recall my earlier post on rings of pig aorta. I have managed to keep them alive quite nicely with DMEM ? many thanks for the tips. Unfortunately, I am still struggling to stain for endothelial adhesion molecules. I have read and received various different opinions on fixation after I have made my cryostat sections. Should the slides be fixed immediately, or air dried first? Acetone or formalin? Any tips would be gratefully received. Thank You, Alistair From Herrick.James <@t> mayo.edu Thu Oct 16 15:45:11 2008 From: Herrick.James <@t> mayo.edu (Herrick, James L.) Date: Thu Oct 16 15:45:17 2008 Subject: [Histonet] Question on Von Kossa Message-ID: Hello everyone, Does anyone have a good Von Kossa stain protocol that they would not mind sharing, on animal bone tissue (femur/tibia) that has been embedded in GMA or MMA (sections are between 5 and 10 ?m thick)? I would appreciate it greatly. Thank you much. Jim From victor <@t> pathology.washington.edu Thu Oct 16 15:50:05 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Oct 16 15:50:11 2008 Subject: [Histonet] PSLIM Update In-Reply-To: <48F76CDE.1CA9.00C8.0@beaumonthospitals.com> References: <48F7A021.1030602@pathology.washington.edu> <48F76CDE.1CA9.00C8.0@beaumonthospitals.com> Message-ID: <48F7A8FD.6020205@pathology.washington.edu> Sharon, The PSLIM holds more than a few slides and they can be swapped in and out easily. We don't know if what happened to our unit was mechanical or software. I personally am not aware of any other small personal slide printers. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Sharon Scalise wrote: > Are there any other small units out there that will print directly on > a slide (one that each tech could have next to their microtome)? We > are looking for one that will handle multiple slides, not one that > needs to be fed slides on at a time. The only one I saw at the NSH > vendor show was the PSLIM and I have not been hearing many positive > reviews. > > > Sharon E. Scalise, HTL (ASCP) > Histology Supervisor > William Beaumont Hospital > Royal Oak, MI 48073 > 248 898-5981 From immrstambo <@t> hotmail.com Thu Oct 16 16:14:11 2008 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Thu Oct 16 16:14:17 2008 Subject: [Histonet] chemical powders Message-ID: Speaking of expiration dates. What does everyone do with their powdered reagents like hematoxylin, ferric choride etc. etc. that dont have an expiration date on the bottle. How do we determine the shelf life? What is the rule? I was always taught as long as it worked it was okay. Is that a safe assumption now days?? Any thoughts or suggestions out there? By the way Happy Friday. Halloween is around the corner!!!! Christine Tambasco St. Mary's Hospital Amsterdam _________________________________________________________________ Want to read Hotmail messages in Outlook? The Wordsmiths show you how. http://windowslive.com/connect/post/wedowindowslive.spaces.live.com-Blog-cns!20EE04FBC541789!167.entry?ocid=TXT_TAGLM_WL_hotmail_092008 From ratliffjack <@t> hotmail.com Thu Oct 16 16:20:16 2008 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Oct 16 16:20:20 2008 Subject: [Histonet] Question on Von Kossa In-Reply-To: References: Message-ID: Jim, Here you go: MMA Thin (5 microns) Sections 1) Deplastify with three changes of Xylenes @ 60C for 30-60 minutes (depending on size of section). You can also use three changes of acetone @ RT for 15-30 minutes. 2) Hydrate tissue from ethanol to DI water (100% EtOH for 5 min, 95% EtOH for 5 min, 70% EtOH for 5 min, DI H2O for 5 min) @ RT. 3) Stain in 5% Silver Nitrate solution @ RT for 5 minutes. Keep tissue and solution protected from light!!!! 4) Three DI water rinses @ RT for one minute each. 5) Develop all bound silver ions in Sodium Carbonate-Formaldehyde solution (5 g sodium carbonate, 25 mL formaldehyde, 75 mL DI water) @ RT for 2 minutes (TIME CRITICAL). 6) Two DI water rinses @ RT for one minute each. 7) Stop reaction and remove all unbound silver ions in Farmer's Diminisher (20 g sodium thiosulfate, 1 g potassium ferricyanide, 210 mL DI water) @ RT for 30 seconds. This solution is only stable for 30-45 minutes so make and use fresh. 8) Wash in running tap water for 20 minutes. 9) Rinse in DI water @ RT for one minute. 10) Counterstain with 5% MacNeal's tetrachrome solution. There are other choices, but this is what I use. 11) Dehydrate to Xylenes and coverslip. I will send you a copy of my detailed protocol. Jack > Date: Thu, 16 Oct 2008 15:45:11 -0500> From: Herrick.James@mayo.edu> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Question on Von Kossa> > Hello everyone,> > Does anyone have a good Von Kossa stain protocol that they would not mind sharing, on animal bone tissue (femur/tibia) that has been embedded in GMA or MMA (sections are between 5 and 10 ?m thick)? I would appreciate it greatly. Thank you much.> > Jim> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Want to read Hotmail messages in Outlook? The Wordsmiths show you how. http://windowslive.com/connect/post/wedowindowslive.spaces.live.com-Blog-cns!20EE04FBC541789!167.entry?ocid=TXT_TAGLM_WL_hotmail_092008 From mwich <@t> 7thwavelabs.com Thu Oct 16 16:26:38 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Thu Oct 16 16:26:45 2008 Subject: [Histonet] myoglobin antibody Message-ID: <62A8156F8071C8439080D626DF8C33A602E506@wave-mail.7thwave.local> Hello, I'm looking for a myoglobin antibody that reacts in rat tissue and works in FFPE tissue. Can anyone recommend a vendor? Thanks for any info. ~Michele This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From SwainFrancesL <@t> uams.edu Fri Oct 17 06:28:58 2008 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Fri Oct 17 06:29:13 2008 Subject: [Histonet] Pig endothelium....again In-Reply-To: References: Message-ID: Hi Allistar: I fix my air-dried ( the tech that cuts the frozens air dries for 60 minutes at room temp prior to placing in a plastic box and freezing the sections. When I get ready to stain them for IHC or regular staining I remove the sections that I need and place them immediately in cold 100% Acetone for 20 minutes. I then proceed with my protocol. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alistair Lindsay Sent: Thursday, October 16, 2008 3:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pig endothelium....again Dear Histonetters, You may recall my earlier post on rings of pig aorta. I have managed to keep them alive quite nicely with DMEM - many thanks for the tips. Unfortunately, I am still struggling to stain for endothelial adhesion molecules. I have read and received various different opinions on fixation after I have made my cryostat sections. Should the slides be fixed immediately, or air dried first? Acetone or formalin? Any tips would be gratefully received. Thank You, Alistair _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From dmikita <@t> wmcnet.org Fri Oct 17 07:54:03 2008 From: dmikita <@t> wmcnet.org (Daryl Mikita) Date: Fri Oct 17 07:54:21 2008 Subject: [Histonet] Open HT position in Wild Wyoming Message-ID: <48F8368B.1875.003D.0@wmcnet.org> Wyoming Medical Center in Casper, Wyoming is looking for a full-time, Day Shift HT with rotating Saturdays (NO nights or Sundays). One year certificate from college or technical school; and three to six months related experience and/or training; or equivalent combination of education and experience. ASCP registered, equivalent or eligible. Wyoming Medical Center is a 205 bed not-for-profit acute care hospital in the center of Wyoming. The Histology Lab. performs approximately 9000 cases per year, and does routine H&E, Special Stains and IHC. We do not perform MOHS procedures, ER/PR or HER2/Neu, at this time. We currently use the Tissue-Tek Glas coverslipper, Tissue-Tek DRS 2000 H&E stainer, and the Dako Autostainer. Wyoming offers many interesting and exciting outdoor experiences for the outdoors person. Wyoming also offers Yellowstone National park, the Flaming Gorge Reservoir and Jackson Hole, WY. Casper has many outdoor activities (Hiking, Fishing, Hunting,....) within an hour drive. This includes several lake for fishing and water sports. We also have a world class trout fishing area within a two hour drive from Casper. If interested please contact me or go to our web page to apply online. http://www.wmcnet.org Daryl A. Mikita, HT(ASCP)cm Wyoming Medical Center Anatomical Pathology 1233 E. 2nd St. Casper, WY 82601 (307) 577-2198 From Bryan.Watson <@t> parkview.com Fri Oct 17 08:21:40 2008 From: Bryan.Watson <@t> parkview.com (Bryan Watson) Date: Fri Oct 17 08:22:10 2008 Subject: [Histonet] B5 fixative Message-ID: <48F85924.ACD8.0085.0@parkview.com> How many still use B5 fixative? And for those who do not, what do you use as a replacement? One of our pathologists is insistent on using B5, and we'd rather try to get away from it. Thanks, Bryan From lblazek <@t> digestivespecialists.com Fri Oct 17 08:38:43 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Oct 17 08:37:53 2008 Subject: [Histonet] B5 fixative In-Reply-To: <48F85924.ACD8.0085.0@parkview.com> References: <48F85924.ACD8.0085.0@parkview.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E3907B6FE1406@IBMB7Exchange.digestivespecialists.com> Try the B5 substitute from Poly Scientific. I did a comparison of the B5 substitutes several years ago and theirs came out on top. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Friday, October 17, 2008 9:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 fixative How many still use B5 fixative? And for those who do not, what do you use as a replacement? One of our pathologists is insistent on using B5, and we'd rather try to get away from it. Thanks, Bryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Fri Oct 17 08:42:24 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Oct 17 08:42:43 2008 Subject: [Histonet] B5 fixative In-Reply-To: <48F85924.ACD8.0085.0@parkview.com> References: <48F85924.ACD8.0085.0@parkview.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA509F7AA@ITSSSXM01V6.one.ads.che.org> B-Plus from BBC has worked well for us. It's Friday!!!!!!!!!!!!! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Friday, October 17, 2008 9:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 fixative How many still use B5 fixative? And for those who do not, what do you use as a replacement? One of our pathologists is insistent on using B5, and we'd rather try to get away from it. Thanks, Bryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Rcartun <@t> harthosp.org Fri Oct 17 08:46:42 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Oct 17 08:46:58 2008 Subject: [Histonet] B5 fixative In-Reply-To: <48F85924.ACD8.0085.0@parkview.com> References: <48F85924.ACD8.0085.0@parkview.com> Message-ID: <48F85F02020000770000638A@gwmail4.harthosp.org> If you are having a hard time with a pathologist regarding the use of B5, get your safety people involved. They will take care of it. We stopped using B5 a very long time ago. We use formalin for all our lymph nodes and bone marrows. No one should be using B5 and manufacturers should stop making it. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Bryan Watson" 10/17/08 9:21 AM >>> How many still use B5 fixative? And for those who do not, what do you use as a replacement? One of our pathologists is insistent on using B5, and we'd rather try to get away from it. Thanks, Bryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lchausse <@t> nmh.org Fri Oct 17 08:47:29 2008 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Fri Oct 17 08:47:53 2008 Subject: [Histonet] Grossing station inquiry Message-ID: Good morning - Are there any sites out there that are using some type of remote pumping system to supply formalin to a gross station with a formalin faucet? If so, would you mind sharing how you've set it up. We're trying to identify if there's a vendor that has a pre-fab unit that would house a formalin source (30 gal drums) as well as provide the pump and connection to the plumbing. Thanks for any input. Leslie ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From cmiller <@t> physlab.com Fri Oct 17 09:43:35 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Oct 17 09:43:45 2008 Subject: [Histonet] Amyloid control tissue Message-ID: <002d01c93066$bc62f890$3d02a8c0@plab.local> Does anyone have any Amyloid tissue they are willing to share?? Thanks, Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From laurie.colbert <@t> huntingtonhospital.com Fri Oct 17 09:49:45 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Oct 17 09:49:50 2008 Subject: [Histonet] B5 fixative Message-ID: <57BE698966D5C54EAE8612E8941D768303F54C68@EXCHANGE3.huntingtonhospital.com> We still use B-5, as our pathologists are also insistent on using it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Friday, October 17, 2008 6:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 fixative How many still use B5 fixative? And for those who do not, what do you use as a replacement? One of our pathologists is insistent on using B5, and we'd rather try to get away from it. Thanks, Bryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Fri Oct 17 09:59:04 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Oct 17 09:59:08 2008 Subject: [Histonet] B5 fixative In-Reply-To: <57BE698966D5C54EAE8612E8941D768303F54C68@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D768303F54C68@EXCHANGE3.huntingtonhospital.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA509F7EF@ITSSSXM01V6.one.ads.che.org> I believe there is a federal law against it. Better check it out! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, October 17, 2008 10:50 AM To: Bryan Watson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] B5 fixative We still use B-5, as our pathologists are also insistent on using it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Friday, October 17, 2008 6:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 fixative How many still use B5 fixative? And for those who do not, what do you use as a replacement? One of our pathologists is insistent on using B5, and we'd rather try to get away from it. Thanks, Bryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From SnyderW <@t> uhcwv.org Fri Oct 17 10:24:51 2008 From: SnyderW <@t> uhcwv.org (Snyder, Wendy) Date: Fri Oct 17 10:25:17 2008 Subject: [Histonet] HT grossing specimens Message-ID: <36379D23B5EE1041BB8A432914FFDBB0012F02CD@UHC-EXCHANGE1.uhc.wvuhs.com> I am looking for HT and or MLT/MT's grossing small specimens. If so, are you getting a difference in your salary/hourly wage for this added responsibility. Thanks, Wendy Snyder, HT(ASCP), MT(AMT), MLT(ASCP) Lead Histology Technician United Hospital Center (304) 624-2652 snyderw@uhcwv.org From tpodawiltz <@t> lrgh.org Fri Oct 17 10:22:20 2008 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Oct 17 10:26:30 2008 Subject: [Histonet] B5 fixative In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA509F7EF@ITSSSXM01V6.one.ads.che.org> References: <57BE698966D5C54EAE8612E8941D768303F54C68@EXCHANGE3.huntingtonhospital.com>, <5D64396A0D4A5346BEBC759022AAEAA509F7EF@ITSSSXM01V6.one.ads.che.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D2C53D4EE@LRGHEXVS1.practice.lrgh.org> We do not use B5 at all. In fact Poly Scientific has discontinued it. For those areas like us that are going Mercury free, the Pathologist would have no choice but to find a replacement fixative. For those who still use B5, just how are you disposing of it? Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [JWeems@sjha.org] Sent: Friday, October 17, 2008 10:59 AM To: Laurie Colbert; Bryan Watson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] B5 fixative I believe there is a federal law against it. Better check it out! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, October 17, 2008 10:50 AM To: Bryan Watson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] B5 fixative We still use B-5, as our pathologists are also insistent on using it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Friday, October 17, 2008 6:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 fixative How many still use B5 fixative? And for those who do not, what do you use as a replacement? One of our pathologists is insistent on using B5, and we'd rather try to get away from it. Thanks, Bryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From JWeems <@t> sjha.org Fri Oct 17 10:30:21 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Oct 17 10:30:25 2008 Subject: [Histonet] HT grossing specimens In-Reply-To: <36379D23B5EE1041BB8A432914FFDBB0012F02CD@UHC-EXCHANGE1.uhc.wvuhs.com> References: <36379D23B5EE1041BB8A432914FFDBB0012F02CD@UHC-EXCHANGE1.uhc.wvuhs.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA509F818@ITSSSXM01V6.one.ads.che.org> Just part of the gross room position here.. But it is called "Processing" according to the latest CAP check list... Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Snyder, Wendy Sent: Friday, October 17, 2008 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT grossing specimens I am looking for HT and or MLT/MT's grossing small specimens. If so, are you getting a difference in your salary/hourly wage for this added responsibility. Thanks, Wendy Snyder, HT(ASCP), MT(AMT), MLT(ASCP) Lead Histology Technician United Hospital Center (304) 624-2652 snyderw@uhcwv.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From JEllin <@t> yumaregional.org Fri Oct 17 10:35:10 2008 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Oct 17 10:35:15 2008 Subject: [Histonet] HT grossing specimens In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA509F818@ITSSSXM01V6.one.ads.che.org> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8011D8ACF@EXCHANGECLUSTER.yumaregional.local> Just for everyone's FYI if you are "Processing" specimen's under CAP regulation, but you are collecting from Medicare, The Medicare regulation is completely different and if audited by Medicare can be subject to huge fines for not meeting the requirement.. Help me out on this one Charles E. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, October 17, 2008 8:30 AM To: Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens Just part of the gross room position here.. But it is called "Processing" according to the latest CAP check list... Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Snyder, Wendy Sent: Friday, October 17, 2008 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT grossing specimens I am looking for HT and or MLT/MT's grossing small specimens. If so, are you getting a difference in your salary/hourly wage for this added responsibility. Thanks, Wendy Snyder, HT(ASCP), MT(AMT), MLT(ASCP) Lead Histology Technician United Hospital Center (304) 624-2652 snyderw@uhcwv.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. From JEllin <@t> yumaregional.org Fri Oct 17 10:37:40 2008 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Oct 17 10:37:45 2008 Subject: [Histonet] HT grossing specimens In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8011D8ACF@EXCHANGECLUSTER.yumaregional.local> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8011D8AD0@EXCHANGECLUSTER.yumaregional.local> But did not finish answering the question, there is a augmentation of pay depending on years of experience and specimen type,, most of these specimens are GI or Prostate so we give a $1.00 augmentation,, but most of the time the PA do the gross and the Pathologist still like doing the large specimens or complex ones. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Friday, October 17, 2008 8:35 AM To: Weems, Joyce; Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens Just for everyone's FYI if you are "Processing" specimen's under CAP regulation, but you are collecting from Medicare, The Medicare regulation is completely different and if audited by Medicare can be subject to huge fines for not meeting the requirement.. Help me out on this one Charles E. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, October 17, 2008 8:30 AM To: Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens Just part of the gross room position here.. But it is called "Processing" according to the latest CAP check list... Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Snyder, Wendy Sent: Friday, October 17, 2008 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT grossing specimens I am looking for HT and or MLT/MT's grossing small specimens. If so, are you getting a difference in your salary/hourly wage for this added responsibility. Thanks, Wendy Snyder, HT(ASCP), MT(AMT), MLT(ASCP) Lead Histology Technician United Hospital Center (304) 624-2652 snyderw@uhcwv.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. From laurie.colbert <@t> huntingtonhospital.com Fri Oct 17 11:09:59 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Oct 17 11:10:04 2008 Subject: [Histonet] B5 fixative Message-ID: <57BE698966D5C54EAE8612E8941D768303F54C9C@EXCHANGE3.huntingtonhospital.com> The waste company that picks up our formalin/xylene/alcohol also picks up the B-5 waste. -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Friday, October 17, 2008 8:22 AM To: Weems, Joyce; Laurie Colbert; Bryan Watson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] B5 fixative We do not use B5 at all. In fact Poly Scientific has discontinued it. For those areas like us that are going Mercury free, the Pathologist would have no choice but to find a replacement fixative. For those who still use B5, just how are you disposing of it? Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [JWeems@sjha.org] Sent: Friday, October 17, 2008 10:59 AM To: Laurie Colbert; Bryan Watson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] B5 fixative I believe there is a federal law against it. Better check it out! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, October 17, 2008 10:50 AM To: Bryan Watson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] B5 fixative We still use B-5, as our pathologists are also insistent on using it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Friday, October 17, 2008 6:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] B5 fixative How many still use B5 fixative? And for those who do not, what do you use as a replacement? One of our pathologists is insistent on using B5, and we'd rather try to get away from it. Thanks, Bryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From gvdobbin <@t> ihis.org Fri Oct 17 11:10:05 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Fri Oct 17 11:10:33 2008 Subject: [Histonet] Paper (teabag) Bx bags-Source? Message-ID: Hi Folks, The archives seem to be temporarily(?) unavailable. Can someone point me in the direction of a supplier of the paper teabag type of Bx bags? Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "And in the end it's not the years in your life that count. It's the life in your years." - Abraham Lincoln ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From mpence <@t> grhs.net Fri Oct 17 11:17:36 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Oct 17 11:17:49 2008 Subject: [Histonet] Paper (teabag) Bx bags-Source? In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A393D@IS-E2K3.grhs.net> http://www.sunburstbottle.com/bags/tea-muslin This what we use. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Friday, October 17, 2008 11:10 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Paper (teabag) Bx bags-Source? Hi Folks, The archives seem to be temporarily(?) unavailable. Can someone point me in the direction of a supplier of the paper teabag type of Bx bags? Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "And in the end it's not the years in your life that count. It's the life in your years." - Abraham Lincoln ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Fri Oct 17 11:25:03 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Oct 17 11:25:06 2008 Subject: [Histonet] Re: B5 fixative Message-ID: Well, this pathologist was dragged screaming away from B-5 - and I've brewed plenty of it out of the dry chemicals myself - but you really can't use it any more. I was surprised to find out that it's still available, but it still is. I haven't found any of the substitutes to be worthwhile. The major advantage of B-5 was that it's a rapid fixative (for small specimens). Lymphoid tissue and marrow really need to be fixed overnight in neutral buffered formalin, for optimal fixation. Bob Richmond Samurai Pathologist Knoxville TN From mickie25 <@t> netzero.net Fri Oct 17 11:40:57 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Fri Oct 17 11:41:12 2008 Subject: [Histonet] Americlear mounting media Message-ID: Dear Histonetters, Could anyone offer what and where to buy mounting media that is compatible with Americlear? Thank you in advance. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From Robert.Lott <@t> TriadHospitals.com Fri Oct 17 11:41:48 2008 From: Robert.Lott <@t> TriadHospitals.com (Lott, Robert) Date: Fri Oct 17 11:42:13 2008 Subject: [Histonet] IHC for Amyloid L Message-ID: <4A3619571D9F6C4CB79C980E91DBE4E67B53E1@TNTRIEXEVS03.triadhospitals.net> Laurie, The "most" common cause in patients with aberrant light chain deposition (i.e. Amyloid L) is multiple myeloma. Staining with kappa and lambda should reveal the Amyloid L. Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Trinity Medical Center/ LabFirst 800 Montclair Road Birmingham, AL 35213-1984 robert.lott@triadhospitals.com 205-592-5387 (office) 205-592-5388 (lab) 205-592-5646 (fax) Message: 3 Date: Wed, 15 Oct 2008 11:56:21 -0700 From: "Laurie Colbert" Subject: [Histonet] IHC for Amyloid L To: Message-ID: <57BE698966D5C54EAE8612E8941D768303F54927@EXCHANGE3.huntingtonhospital.c om> Content-Type: text/plain; charset="us-ascii" Does anyone know of a reference lab that does an IHC stain for Amyloid L (amyloid light chain)? I can find labs that do Amyloid A and Amyloid P, but not Amyloid L. Laurie Colbert Huntington Hospital Pasadena, CA -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From kbradshaw <@t> lcpath.com Fri Oct 17 11:55:16 2008 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Fri Oct 17 11:55:21 2008 Subject: [Histonet] RE: HT grossing specimens In-Reply-To: <36379D23B5EE1041BB8A432914FFDBB0012F02CD@UHC-EXCHANGE1.uhc.wvuhs.com> Message-ID: <40912dc15597d94ca8b19eb508dd09ba@mail2.lcpath.com> Compensation for techs who can and do gross is 12% higher than non-grossing techs. As far as "processing"....We added that to our grossing manual, but if you are held to CLIA standards it is irrelevant. In my opinion, most labs will be better served to follow the guidelines of well documented training, direct or indirect supervision, a comprehensive list of what specimens can be grossed in by personnel other than pathologists, and yearly evaluation of all employees who gross or "process". There is a great deal of added responsibility here and I believe we need to do everything we can to protect the techs who do it. Kari L. Bradshaw HT(ASCP) Laboratory Manager Lower Columbia Pathologists (360)425-5620 kbradshaw@lcpath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Snyder, Wendy Sent: Friday, October 17, 2008 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT grossing specimens I am looking for HT and or MLT/MT's grossing small specimens. If so, are you getting a difference in your salary/hourly wage for this added responsibility. Thanks, Wendy Snyder, HT(ASCP), MT(AMT), MLT(ASCP) Lead Histology Technician United Hospital Center (304) 624-2652 snyderw@uhcwv.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Fri Oct 17 11:56:49 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Oct 17 11:56:57 2008 Subject: [Histonet] Paper (teabag) Bx bags-Source? In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A393D@IS-E2K3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C017A393D@IS-E2K3.grhs.net> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82E8F@EMAIL.archildrens.org> We use the same source as Mike. They are great bags...and cheap too! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, October 17, 2008 11:18 AM To: Greg Dobbin; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paper (teabag) Bx bags-Source? http://www.sunburstbottle.com/bags/tea-muslin This what we use. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Friday, October 17, 2008 11:10 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Paper (teabag) Bx bags-Source? Hi Folks, The archives seem to be temporarily(?) unavailable. Can someone point me in the direction of a supplier of the paper teabag type of Bx bags? Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "And in the end it's not the years in your life that count. It's the life in your years." - Abraham Lincoln ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From TMcNemar <@t> lmhealth.org Fri Oct 17 12:13:28 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Oct 17 12:13:13 2008 Subject: [Histonet] HT grossing specimens In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8011D8AD0@EXCHANGECLUSTER.yumaregional.local> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E046@lmhsmail.lmhealth.org> Is that $1 per specimen or $1 per hour? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jesus Ellin Sent: Friday, October 17, 2008 11:38 AM To: Jesus Ellin; Weems, Joyce; Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens But did not finish answering the question, there is a augmentation of pay depending on years of experience and specimen type,, most of these specimens are GI or Prostate so we give a $1.00 augmentation,, but most of the time the PA do the gross and the Pathologist still like doing the large specimens or complex ones. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Friday, October 17, 2008 8:35 AM To: Weems, Joyce; Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens Just for everyone's FYI if you are "Processing" specimen's under CAP regulation, but you are collecting from Medicare, The Medicare regulation is completely different and if audited by Medicare can be subject to huge fines for not meeting the requirement.. Help me out on this one Charles E. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, October 17, 2008 8:30 AM To: Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens Just part of the gross room position here.. But it is called "Processing" according to the latest CAP check list... Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Snyder, Wendy Sent: Friday, October 17, 2008 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT grossing specimens I am looking for HT and or MLT/MT's grossing small specimens. If so, are you getting a difference in your salary/hourly wage for this added responsibility. Thanks, Wendy Snyder, HT(ASCP), MT(AMT), MLT(ASCP) Lead Histology Technician United Hospital Center (304) 624-2652 snyderw@uhcwv.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Fri Oct 17 12:46:47 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri Oct 17 12:46:55 2008 Subject: [Histonet] HT grossing specimens References: <36379D23B5EE1041BB8A432914FFDBB0012F02CD@UHC-EXCHANGE1.uhc.wvuhs.com> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120187@uwhis-xchng3.uwhis.hosp.wisc.edu> That would be a no. In fact we are loosing our grossing tech and our supervisor doesn't want to post the position as she thinks the rest of us have enough 'down time' to cover it. So there are three of us trying to switch our schedules around to do the gross too. I love the "...and other duties as needed." bit in our job descriptions. Sorry I'm being a bear. We are actually loosing 2 people simultaneously. When there are only 8 of us (fully staffed) to begin with, and that includes the transcriptionists. :p Claire ________________________________ I am looking for HT and or MLT/MT's grossing small specimens. If so, are you getting a difference in your salary/hourly wage for this added responsibility. Thanks, Wendy Snyder, HT(ASCP), MT(AMT), MLT(ASCP) Lead Histology Technician United Hospital Center (304) 624-2652 snyderw@uhcwv.org From JEllin <@t> yumaregional.org Fri Oct 17 13:14:58 2008 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Oct 17 13:15:05 2008 Subject: [Histonet] HT grossing specimens In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E046@lmhsmail.lmhealth.org> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8011D8AD4@EXCHANGECLUSTER.yumaregional.local> $1.00 dollar per hour Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: Tom McNemar [mailto:TMcNemar@lmhealth.org] Sent: Friday, October 17, 2008 10:13 AM To: Jesus Ellin; Weems, Joyce; Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens Is that $1 per specimen or $1 per hour? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jesus Ellin Sent: Friday, October 17, 2008 11:38 AM To: Jesus Ellin; Weems, Joyce; Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens But did not finish answering the question, there is a augmentation of pay depending on years of experience and specimen type,, most of these specimens are GI or Prostate so we give a $1.00 augmentation,, but most of the time the PA do the gross and the Pathologist still like doing the large specimens or complex ones. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Friday, October 17, 2008 8:35 AM To: Weems, Joyce; Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens Just for everyone's FYI if you are "Processing" specimen's under CAP regulation, but you are collecting from Medicare, The Medicare regulation is completely different and if audited by Medicare can be subject to huge fines for not meeting the requirement.. Help me out on this one Charles E. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, October 17, 2008 8:30 AM To: Snyder, Wendy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT grossing specimens Just part of the gross room position here.. But it is called "Processing" according to the latest CAP check list... Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Snyder, Wendy Sent: Friday, October 17, 2008 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT grossing specimens I am looking for HT and or MLT/MT's grossing small specimens. If so, are you getting a difference in your salary/hourly wage for this added responsibility. Thanks, Wendy Snyder, HT(ASCP), MT(AMT), MLT(ASCP) Lead Histology Technician United Hospital Center (304) 624-2652 snyderw@uhcwv.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. From Jenee.S.Odani <@t> hawaii.gov Fri Oct 17 13:21:27 2008 From: Jenee.S.Odani <@t> hawaii.gov (Jenee.S.Odani@hawaii.gov) Date: Fri Oct 17 13:21:32 2008 Subject: [Histonet] fixative for brains? In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8011D8AD4@EXCHANGECLUSTER.yumaregional.local> Message-ID: I'm trying to revise our laboratories SOPs. They currently are using 20% NBF to fix mammalian brains and alcohol-formalin to fix avian brains. In the past, I've used 10% NBF for all brains. Does anyone have any input on this? I'd like to keep things simple, if possible, and just use one fixative. Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 From kynemitz <@t> travmax.com Fri Oct 17 13:35:21 2008 From: kynemitz <@t> travmax.com (Kyla Nemitz) Date: Fri Oct 17 13:35:54 2008 Subject: [Histonet] Histo opening in San Antonio, TX Message-ID: <9416D9FA37C1C04FA83D69684E95D2E71E4DE602@exbk2.maxhealth.com> Happy Friday! I have a new position open and wanted to keep you all informed. A facility I work closely with in San Antonio, TX has just asked for my help in finding a Histo tech. Basic histology, IHC experience and ASCP preferred. Salary range $48,000 - $53,000. Potential for relocation aid and sign on bonuses! If you or anyone you know would be interested in this position or any others I have available, please contact me. I specialize in perm placement, temp-to-perm and travel for laboratory professionals. If you have any questions about my company, services or the histology market, please feel free to give me a call or send an email. Thank you, hope to hear from you soon and have a great weekend! Kyla Nemitz TravelMax Medical Professionals - Maxim Healthcare Tel: 888.800.1855 or 813.371.5175 Fax: 800.294.1248 Search our jobs or apply online at: www.TravelMaxAllied.com From SHargrove <@t> urhcs.org Fri Oct 17 14:23:51 2008 From: SHargrove <@t> urhcs.org (SHargrove@urhcs.org) Date: Fri Oct 17 14:24:13 2008 Subject: [Histonet] Susie Hargrove is out of the office. Message-ID: I will be out of the office starting 10/17/2008 and will not return until 10/27/2008. I will respond to your message when I return. If immediate assistance is needed please call 3198. From lchen <@t> mednet.ucla.edu Fri Oct 17 15:36:04 2008 From: lchen <@t> mednet.ucla.edu (Leslie Chen) Date: Fri Oct 17 15:36:19 2008 Subject: [Histonet] gluteraldehyde and IHC Message-ID: <002201c93097$ffe6cac0$3ba62f0a@DHGLABC99E93DF> Does anyone know if the gluteraldehyde would also obliterate immunoflorescence staining as well? Does gluteraldehyde always affect IHC staining? I have some LacZ stained embryos that have been paraffin processed and I was going to try to optimize flourescence staining on them. Thanks. Leslie Message: 7 Date: Thu, 16 Oct 2008 16:02:18 -0400 From: "Andrea Hooper" Subject: [Histonet] X-Gal stain of bone To: "'Markus F. Meyenhofer'" Cc: Histonet Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed Dear Markus, We do X-Gal staining on bone and in the best scenario the animals are perfused with glutaraldehyde (GA). PFA tends to quench the beta-gal activity and the results may not be 100% accurate (underestimation of positivity). Though if you want to do IHC on these samples, I am sure you know that the GA fix obliterates that possibility. Also it is critical to decalcify in EDTA rather than an acid decal. Let me know if you need more details, Andrea ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From pieronelva01 <@t> bigpond.com Fri Oct 17 16:59:16 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Fri Oct 17 16:59:24 2008 Subject: [Histonet] B5 fixative References: <48F85924.ACD8.0085.0@parkview.com> <48F85F02020000770000638A@gwmail4.harthosp.org> Message-ID: I agree. The Occ Health and SAfety representative is your best bet to consign the B5 to history. Piero Nelva Anatomical pathology Monash Medical Centre Australia ----- Original Message ----- From: "Richard Cartun" To: ; "Bryan Watson" Sent: Saturday, October 18, 2008 12:46 AM Subject: Re: [Histonet] B5 fixative If you are having a hard time with a pathologist regarding the use of B5, get your safety people involved. They will take care of it. We stopped using B5 a very long time ago. We use formalin for all our lymph nodes and bone marrows. No one should be using B5 and manufacturers should stop making it. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Bryan Watson" 10/17/08 9:21 AM >>> How many still use B5 fixative? And for those who do not, what do you use as a replacement? One of our pathologists is insistent on using B5, and we'd rather try to get away from it. Thanks, Bryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.8.1/1731 - Release Date: 10/17/2008 7:01 PM From amosbrooks <@t> gmail.com Fri Oct 17 23:15:05 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Oct 17 23:15:09 2008 Subject: [Histonet] Question on Von Kossa Message-ID: <582736990810172115y43e24d8dq493316fbcc97eaac@mail.gmail.com> James, Try this: once the sections are brought to water, 5% silver nitrate in either bright sunlight or a 60-100 watt incandescent light bulb for 30 min (check for browning of the control tissue adjust time as needed). Rinse well in distilled water. 5% Sodium Thiosulfate for 1 min. Rinse and counterstain with nuclear fast red or whatever you think would look cool :-). Dehydrate, clear and coverslip the slides. Of course, as with any silver stain use acid cleaned glassware and gloves unless you like watching your fingers turn black in the sunlight. Really well decalcified tissue usually has the Calcium washed out. Try a calcified breast or something that wasn't decalcified. This causes microtomists to curse a lot, but it makes great VonKossa controls. Have fun, Amos Message: 10 Date: Thu, 16 Oct 2008 15:45:11 -0500 From: "Herrick, James L." Subject: [Histonet] Question on Von Kossa To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello everyone, Does anyone have a good Von Kossa stain protocol that they would not mind sharing, on animal bone tissue (femur/tibia) that has been embedded in GMA or MMA (sections are between 5 and 10 ?m thick)? I would appreciate it greatly. Thank you much. Jim From billions1998 <@t> hotmail.com Sun Oct 19 02:35:18 2008 From: billions1998 <@t> hotmail.com (Sinoera Tech) Date: Sun Oct 19 03:08:20 2008 Subject: [Histonet] Re: Alcian Yellow In-Reply-To: <6CBA6DC98A079D408C87250591D9DFB802684AAA@bruexchange.digestivespecialists.com> References: <6CBA6DC98A079D408C87250591D9DFB802684AAA@bruexchange.digestivespecialists.com> Message-ID: Dear Sir, We can supply you with Alcian Yellow and Alcian Blue 8GX. Kind Regards. - Minggeng Wang, Ph.D / President SUZHOU SINOERA CHEM CO., LTD. 125 Binhe Road Suzhou New & Hi-Tech District 215011 China Fax: +86 512 68258994 Tel: +86 512 68246939 http://www.sinoeratech.com From: Blazek, Linda Sent: Friday, September 22, 2006 10:12 PM To: scott142@comcast.net ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Alcian Yellow **HELP** I'd also be interested! Want to share? Or if anyone knows of a good substitute that info would be appreciated too. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of scott142@comcast.net Sent: Friday, September 22, 2006 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alcian Yellow **HELP** I routinely run a procedure that works very well with old lots of Alcian Yellow. Unfortunately I have no more lots of old Alcian Yellow. If anyone has inventory of Alcian Yellow that they do not use, I would be very eager to purchase it from you. Thanks you... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From conniegrubaugh <@t> hotmail.com Sun Oct 19 11:47:58 2008 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Sun Oct 19 11:48:01 2008 Subject: [Histonet] Re: Alcian Yellow In-Reply-To: References: <6CBA6DC98A079D408C87250591D9DFB802684AAA@bruexchange.digestivespecialists.com> Message-ID: American Master Tech has Alcian Yellow Connie G. > From: billions1998@hotmail.com > To: lblazek@digestivespecialists.com; scott142@comcast.net; histonet@lists.utsouthwestern.edu > Date: Sun, 19 Oct 2008 15:35:18 +0800 > CC: > Subject: [Histonet] Re: Alcian Yellow > > > Dear Sir, > > We can supply you with Alcian Yellow and Alcian Blue 8GX. > > Kind Regards. > - > Minggeng Wang, Ph.D / President > SUZHOU SINOERA CHEM CO., LTD. > 125 Binhe Road > Suzhou New & Hi-Tech District > 215011 China > Fax: +86 512 68258994 > Tel: +86 512 68246939 > http://www.sinoeratech.com > > > > From: Blazek, Linda > Sent: Friday, September 22, 2006 10:12 PM > To: scott142@comcast.net ; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Alcian Yellow **HELP** > > > I'd also be interested! Want to share? Or if anyone knows of a good > substitute that info would be appreciated too. > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > scott142@comcast.net > Sent: Friday, September 22, 2006 9:48 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Alcian Yellow **HELP** > > I routinely run a procedure that works very well with old lots of Alcian > Yellow. Unfortunately I have no more lots of old Alcian Yellow. If > anyone has inventory of Alcian Yellow that they do not use, I would be > very eager to purchase it from you. > > Thanks you... > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ You live life beyond your PC. So now Windows goes beyond your PC. http://clk.atdmt.com/MRT/go/115298556/direct/01/ From pruegg <@t> ihctech.net Sun Oct 19 12:09:18 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Oct 19 12:09:29 2008 Subject: [Histonet] Question on Von Kossa In-Reply-To: <582736990810172115y43e24d8dq493316fbcc97eaac@mail.gmail.com> References: <582736990810172115y43e24d8dq493316fbcc97eaac@mail.gmail.com> Message-ID: <0190942AC6114F07978BD3775EC76F49@ihctechq9h2qof> That is how I do VK, I put it in the window for 20 min or so. I do a special H&E counterstain on mine, using an aqueous eosin. The eosin will light up the osteoid by fluorescing under uv light. We use this with a image analysis system to measure total area, calcified bone area (light scope, from the black silver stain) osteoid seam thickness (fluorescent scope using the eosin fluorescent property, everything else will be dark except the osteoid seams) if you labeled the bone with a fluorchrome you can just look at an unstained section to measure area between two labels. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, October 17, 2008 10:15 PM To: Herrick.James@mayo.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Question on Von Kossa James, Try this: once the sections are brought to water, 5% silver nitrate in either bright sunlight or a 60-100 watt incandescent light bulb for 30 min (check for browning of the control tissue adjust time as needed). Rinse well in distilled water. 5% Sodium Thiosulfate for 1 min. Rinse and counterstain with nuclear fast red or whatever you think would look cool :-). Dehydrate, clear and coverslip the slides. Of course, as with any silver stain use acid cleaned glassware and gloves unless you like watching your fingers turn black in the sunlight. Really well decalcified tissue usually has the Calcium washed out. Try a calcified breast or something that wasn't decalcified. This causes microtomists to curse a lot, but it makes great VonKossa controls. Have fun, Amos Message: 10 Date: Thu, 16 Oct 2008 15:45:11 -0500 From: "Herrick, James L." Subject: [Histonet] Question on Von Kossa To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello everyone, Does anyone have a good Von Kossa stain protocol that they would not mind sharing, on animal bone tissue (femur/tibia) that has been embedded in GMA or MMA (sections are between 5 and 10 ?m thick)? I would appreciate it greatly. Thank you much. Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sjchtascp <@t> yahoo.com Sun Oct 19 15:56:30 2008 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Sun Oct 19 15:56:33 2008 Subject: [Histonet] Madison WI Message-ID: <160679.26224.qm@web38205.mail.mud.yahoo.com> HT(ASCP) 14 years experience looking for employment in Madison WI. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From hana444 <@t> gmail.com Mon Oct 20 03:29:54 2008 From: hana444 <@t> gmail.com (Hana Peter) Date: Mon Oct 20 03:30:03 2008 Subject: [Histonet] eosin pH Message-ID: <48FC4182.8050507@gmail.com> Hi! Can someone tell me the right pH for eosin y 1% aqueous solution? The one I made from powder has pH 6.3. Is this OK? The commercial one we usually buy is a bit lower (5.7). Thank you in advance! Hana Peter From adam.boanas <@t> renovo.com Mon Oct 20 05:07:56 2008 From: adam.boanas <@t> renovo.com (Adam Boanas) Date: Mon Oct 20 05:08:00 2008 Subject: [Histonet] Elastin Aldehyde Fuchsin Message-ID: <22ADCE0141FB0145A580E85CE3C70BD6B5690B@ex2003.winserv.renovo-ltd.com> Hello, I have been using Gomori`s Aldehyde Fuchsin to stain human skin for elastin. The stain works fine and is clear and specific. I am using light green as a counterstain. My problem is that once stained, the slides have an excess granular purple "dotted" appearance upon the entire surface of the slide. This is not limited to the tissue position upon the slide, but across the clear glass too. We have tried using both superfrost plus charged slides and uncharged slides but the problem still is present. This precipitate is spoiling an otherwise great stain. Does anyone have any idea about what this is and how it could be prevented? (Our next guess would be to re-filter the fuchsin solution prior to use every time) Many thanks Adam Adam Boanas Histology Manager Renovo Ltd PLEASE NOTE OUR TELEPHONE NUMBERS ARE CHANGING -PLEASE AMEND YOUR RECORDS: >From Monday 07/06/2008 our Telephone number will be as follows: Switchboard: 0161 276 7100 extension 7716 LEGAL NOTICE This e-mail is from Renovo Group plc (Registered No 5427608 England). The information contained in this e-mail and any files transmitted with it are confidential, may be privileged and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient any disclosure, copying, retransmission, distribution, dissemination, action taken or omitted to be taken in reliance upon this e-mail is strictly prohibited and may be unlawful. If you have received this communication in error please notify the sender by e-mail or by telephone on +44(0)161 276 7100, delete it from your system and destroy any copies of it. Please note that e-mails from Renovo Group plc pass through a virus checker but e-mails can be intercepted or affected by different viruses. Neither Renovo Group plc nor the sender accepts any liability or responsibility for viruses or other material introduced with this message and the recipient should ensure that the e-mail and attachments (if any) are actually virus free. Any views expressed by an individual within this e-mail do not necessarily represent the views of Renovo Group plc. No contract may be concluded on behalf of Renovo Group plc by means of e-mail communications. Renovo Group plc, Manchester Incubator Building, 48 Grafton Street, Manchester, M13 9XX, UK Tel: +44(0)161 276 7100 Fax: +44(0)161 276 7200 Website: http://www.renovo.com From annigyg <@t> gmail.com Mon Oct 20 07:45:23 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Mon Oct 20 07:45:29 2008 Subject: Fwd: [Histonet] Question on Von Kossa In-Reply-To: References: <48fc7645.29578c0a.2353.1e04SMTPIN_ADDED@mx.google.com> Message-ID: no - thats the really cool thing - think about it... its that part of the bone which is usually quite hard but softens with stewing/cooking and could be bitten off and chewed (if desired) no demineralisation has taken place yet it is soft enough to slice with a blade and once processed it cuts like butter on the microtome i also have an amazing source of GMS control for fungus - an old mildewed orange!!! same process applies - slice off a piece, process and embed - stains on PAS and GMS!!! quite stunning a pal of mine says that fish liver makes and amazing Oil red 'O' QC but have not yet tried that one i also hunt for liver QC material here in a country which does not do autopsies - we trot off to the local butcher with a small jar of formalin and pop a small slice of fresh beef liver into the formalin - am sure that other liver would work just as wekk some may argue that it is animal tissue but in my opinion it it not the tissue but the glycogen we need to demo - so its not an issue - my Paths are happy so im happy 2008/10/20 Patsy Ruegg Do you have to decal it? > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech > > 12635 Montview Blvd. #215 > > Aurora, CO 80045 > > 720-859-4060 > > fax 720-859-4110 > > pruegg@ihctech.net > > www.ihctech.net > > www.ihcrg.org > > > > > ------------------------------ > > *From:* Anne van Binsbergen [mailto:annigyg@gmail.com] > *Sent:* Sunday, October 19, 2008 9:48 PM > *To:* Patsy Ruegg > *Subject:* Re: [Histonet] Question on Von Kossa > > > > for an excellent VK control try this: > > > > after you have eaten all the meat from the bones of a chicken stew - keep > the thigh bones. > > Cut off the soft part on the end, pop into a casette, process as usual and > viola!!! > > Try it for yourself - works like a charm > > ;)) > > Annie > > > > > > > > 2008/10/19 Patsy Ruegg > > That is how I do VK, I put it in the window for 20 min or so. I do a > special H&E counterstain on mine, using an aqueous eosin. The eosin will > light up the osteoid by fluorescing under uv light. We use this with a > image analysis system to measure total area, calcified bone area (light > scope, from the black silver stain) osteoid seam thickness (fluorescent > scope using the eosin fluorescent property, everything else will be dark > except the osteoid seams) if you labeled the bone with a fluorchrome you > can > just look at an unstained section to measure area between two labels. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos > Brooks > Sent: Friday, October 17, 2008 10:15 PM > To: Herrick.James@mayo.edu; histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question on Von Kossa > > James, > Try this: once the sections are brought to water, 5% silver nitrate in > either bright sunlight or a 60-100 watt incandescent light bulb for 30 min > (check for browning of the control tissue adjust time as needed). Rinse > well > in distilled water. 5% Sodium Thiosulfate for 1 min. Rinse and counterstain > with nuclear fast red or whatever you think would look cool :-). Dehydrate, > clear and coverslip the slides. > Of course, as with any silver stain use acid cleaned glassware and gloves > unless you like watching your fingers turn black in the sunlight. Really > well decalcified tissue usually has the Calcium washed out. Try a calcified > breast or something that wasn't decalcified. This causes microtomists to > curse a lot, but it makes great VonKossa controls. > > Have fun, > Amos > > Message: 10 > Date: Thu, 16 Oct 2008 15:45:11 -0500 > From: "Herrick, James L." > Subject: [Histonet] Question on Von Kossa > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Hello everyone, > > Does anyone have a good Von Kossa stain protocol that they would not mind > sharing, on animal bone tissue (femur/tibia) that has been embedded in GMA > or MMA (sections are between 5 and 10 ?m thick)? I would appreciate it > greatly. Thank you much. > > Jim > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > -- > Anne van Binsbergen (Hope) > Abu Dhabi > UAE > -- Anne van Binsbergen (Hope) Abu Dhabi UAE -- Anne van Binsbergen (Hope) Abu Dhabi UAE From rjbuesa <@t> yahoo.com Mon Oct 20 07:59:45 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 20 07:59:49 2008 Subject: [Histonet] eosin pH In-Reply-To: <48FC4182.8050507@gmail.com> Message-ID: <478046.83469.qm@web65709.mail.ac4.yahoo.com> The eosin should be acidic and you get to that point adding 1% aq. sol. of acetic acid to you prepared solution, at a rate of 1 mL acetic/100 mL of eosin solution. Ren? J. --- On Mon, 10/20/08, Hana Peter wrote: From: Hana Peter Subject: [Histonet] eosin pH To: histonet@lists.utsouthwestern.edu Date: Monday, October 20, 2008, 4:29 AM Hi! Can someone tell me the right pH for eosin y 1% aqueous solution? The one I made from powder has pH 6.3. Is this OK? The commercial one we usually buy is a bit lower (5.7). Thank you in advance! Hana Peter _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From jo-ann.bader <@t> mcgill.ca Mon Oct 20 08:07:24 2008 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Mon Oct 20 08:07:36 2008 Subject: [Histonet] Acidified Hematoxylin Message-ID: Good Morning Everyone, We are in dire straights We are coming to the end of out Acidified Harris Hematoxylin and I was just informed that the company that produces it will not start production until November. We will never make it. Does anyone know where I can get some? Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: jo-ann.bader@mcgill.ca From renafail <@t> bellsouth.net Mon Oct 20 09:02:13 2008 From: renafail <@t> bellsouth.net (renafail@bellsouth.net) Date: Mon Oct 20 09:02:17 2008 Subject: [Histonet] Elastin Aldehyde Fuchsin In-Reply-To: <22ADCE0141FB0145A580E85CE3C70BD6B5690B@ex2003.winserv.renovo-ltd.com> Message-ID: <102020081402.11584.48FC8F65000436C100002D4022243651029B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net> The solution does need to be filtered before each use Rena Fail -------------- Original message from "Adam Boanas" : -------------- > Hello, > > > > I have been using Gomori`s Aldehyde Fuchsin to stain human skin for > elastin. The stain works fine and is clear and specific. I am using > light green as a counterstain. My problem is that once stained, the > slides have an excess granular purple "dotted" appearance upon the > entire surface of the slide. This is not limited to the tissue position > upon the slide, but across the clear glass too. We have tried using both > superfrost plus charged slides and uncharged slides but the problem > still is present. This precipitate is spoiling an otherwise great stain. > Does anyone have any idea about what this is and how it could be > prevented? (Our next guess would be to re-filter the fuchsin solution > prior to use every time) > > > > Many thanks > > Adam > > > > Adam Boanas > Histology Manager > Renovo Ltd > > PLEASE NOTE OUR TELEPHONE NUMBERS ARE CHANGING -PLEASE AMEND YOUR > RECORDS: > > >From Monday 07/06/2008 our Telephone number will be as follows: > > Switchboard: 0161 276 7100 extension 7716 > > > > LEGAL NOTICE > This e-mail is from Renovo Group plc (Registered No 5427608 England). The > information contained in this e-mail and any files transmitted with > it are confidential, may be privileged and are intended solely for the use of > the individual or entity to whom they are addressed. If you are > not the intended recipient any disclosure, copying, retransmission, > distribution, dissemination, action taken or omitted to be taken in reliance > upon this e-mail is strictly prohibited and may be unlawful. If you have > received this communication in error please notify the sender by e-mail > or by telephone on +44(0)161 276 7100, delete it from your system and destroy > any copies of it. > Please note that e-mails from Renovo Group plc pass through a virus checker but > e-mails can be intercepted or affected by different viruses. > Neither Renovo Group plc nor the sender accepts any liability or responsibility > for viruses or other material introduced with this message and > the recipient should ensure that the e-mail and attachments (if any) are > actually virus free. > Any views expressed by an individual within this e-mail do not necessarily > represent the views of Renovo Group plc. No contract may be concluded > on behalf of Renovo Group plc by means of e-mail communications. > Renovo Group plc, Manchester Incubator Building, 48 Grafton Street, Manchester, > M13 9XX, UK > Tel: +44(0)161 276 7100 Fax: +44(0)161 276 7200 > Website: http://www.renovo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bryan.Watson <@t> parkview.com Mon Oct 20 09:04:05 2008 From: Bryan.Watson <@t> parkview.com (Bryan Watson) Date: Mon Oct 20 09:04:56 2008 Subject: [Histonet] B5 fixative In-Reply-To: References: <48F85924.ACD8.0085.0@parkview.com> <48F85F02020000770000638A@gwmail4.harthosp.org> Message-ID: <48FC5795.ACD8.0085.0@parkview.com> For all of the listed B5 substitutes mentioned, how are they disposed of and are they significantly safer? >>> "Piero Nelva" 10/17/2008 17:59 >>> I agree. The Occ Health and SAfety representative is your best bet to consign the B5 to history. Piero Nelva Anatomical pathology Monash Medical Centre Australia ----- Original Message ----- From: "Richard Cartun" To: ; "Bryan Watson" Sent: Saturday, October 18, 2008 12:46 AM Subject: Re: [Histonet] B5 fixative If you are having a hard time with a pathologist regarding the use of B5, get your safety people involved. They will take care of it. We stopped using B5 a very long time ago. We use formalin for all our lymph nodes and bone marrows. No one should be using B5 and manufacturers should stop making it. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Bryan Watson" 10/17/08 9:21 AM >>> How many still use B5 fixative? And for those who do not, what do you use as a replacement? One of our pathologists is insistent on using B5, and we'd rather try to get away from it. Thanks, Bryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.8.1/1731 - Release Date: 10/17/2008 7:01 PM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tgenade <@t> gmail.com Mon Oct 20 09:07:07 2008 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Mon Oct 20 09:07:18 2008 Subject: [Histonet] getting rid of biotin-avidin background Message-ID: Hi all, I have a problem and think I have figured out a solution to it but need some expert advise. I'm using biotinylated GS isolectin to stain frozen brain sections for microglia. The GS isolectin is working very well but the avadin-fluourophore is binding to the endogenous biotin in the tissue causing a lot of back ground. I'm thinking of fist blocking the sections by incubating it with unlabled avidin and then following it with another blocking step with unlabled biotin so that all the endogenous biotin is bound to avidin and the un-endogenous biotin bound sites of the avidin are then bound to unlabled biotin. This would then be followed by the GS Isolectin incubation etc... Can anyone suggest a reasonable starting concentration for the first biotin and avidin blocking steps? Do you think this will work at all? Kind regards -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@freeshell.org tel: +27-84-632-1925 (c) ******************************************************************************** "For there is one God, and there is one mediator between God and men, the man Christ Jesus, who gave himself as a ransom for all." 1 Timothy 2:5-6 From godsgalnow <@t> aol.com Mon Oct 20 09:14:04 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Oct 20 09:14:35 2008 Subject: [Histonet] help-fibrotic tissue Message-ID: <8CB00D53E12934B-784-2540@MBLK-M27.sysops.aol.com> I need to finally call on the experts.? I have tried all of the tricks up my sleeve to cut extremely fibrotic Cerivical tissue.? It is not calcified, just fibrotic...it is like cutting glass. Help. Roxanne From anh2006 <@t> med.cornell.edu Mon Oct 20 09:13:18 2008 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Mon Oct 20 09:15:42 2008 Subject: [Histonet] getting rid of biotin-avidin background In-Reply-To: References: Message-ID: <861309140-1224512133-cardhu_decombobulator_blackberry.rim.net-1377979103-@bxe270.bisx.prod.on.blackberry> Why not use an avidin-biotin blocking kit (like those from Vector or Dako for example). They work well, are optiimized already and aren't that expensive. -----Original Message----- From: Tyrone Genade Date: Mon, 20 Oct 2008 16:07:07 To: Subject: [Histonet] getting rid of biotin-avidin background Hi all, I have a problem and think I have figured out a solution to it but need some expert advise. I'm using biotinylated GS isolectin to stain frozen brain sections for microglia. The GS isolectin is working very well but the avadin-fluourophore is binding to the endogenous biotin in the tissue causing a lot of back ground. I'm thinking of fist blocking the sections by incubating it with unlabled avidin and then following it with another blocking step with unlabled biotin so that all the endogenous biotin is bound to avidin and the un-endogenous biotin bound sites of the avidin are then bound to unlabled biotin. This would then be followed by the GS Isolectin incubation etc... Can anyone suggest a reasonable starting concentration for the first biotin and avidin blocking steps? Do you think this will work at all? Kind regards -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@freeshell.org tel: +27-84-632-1925 (c) ******************************************************************************** "For there is one God, and there is one mediator between God and men, the man Christ Jesus, who gave himself as a ransom for all." 1 Timothy 2:5-6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CHRISH <@t> HEALTHCARESCOUTS.COM Mon Oct 20 09:16:54 2008 From: CHRISH <@t> HEALTHCARESCOUTS.COM (Chris Handrahan) Date: Mon Oct 20 09:24:28 2008 Subject: [Histonet] Histology Openings in Seattle Message-ID: Looking for ASCP certified Histotechs for the following openings day position, 7-3:30pm 2nd shift position, 6pm - 2:30am - pays $3 an hour shift differential 3rd shift position, 11pm - 7:30am - pays $10 an hour shift differential Pathologists' Assistant Lead- 10am-6:30pm, M-F IHC Lead - day position, at least 5+ yrs experience including 2 yrs in a lead role. These are all full time permanent positions. These are immediate needs so please contact me today if you are interested! Chris Handrahan Managing Director of Allied Health Healthcare Scouts 800-708-0605 office 321-231-5427 cell chrish@healthcarescouts.com www.healthcarescouts.com From ABonaiuto <@t> sarapath.com Mon Oct 20 09:24:01 2008 From: ABonaiuto <@t> sarapath.com (Alice Bonaiuto) Date: Mon Oct 20 09:26:12 2008 Subject: [Histonet] Job Opening Message-ID: Histology job opening for routine bench work &/ or IHC at Sarsota Pathology/Sarasota, Fl. For more information go to www.sarapath.com . Fax resumes to :(941) 362-8992 or HR@sarapath.com Thanks, Alice Bonaiuto, IHC Technical Specialist From jo-ann.bader <@t> mcgill.ca Mon Oct 20 09:41:41 2008 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Mon Oct 20 09:42:02 2008 Subject: [Histonet] Baker's Fluid Message-ID: Does anyone have the recepe fpr Baker's fluid? Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: jo-ann.bader@mcgill.ca From JWeems <@t> sjha.org Mon Oct 20 10:16:59 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Oct 20 10:17:35 2008 Subject: [Histonet] Baker's Fluid In-Reply-To: References: Message-ID: <5D64396A0D4A5346BEBC759022AAEAA50E3799@ITSSSXM01V6.one.ads.che.org> This was in the archives. Hope it helps! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax On Wed, 24 May 2000, Barry Rittman wrote: > what you need is "Mollifex" sold by BDH You shouldn't use secret mixtures, but Mollifex isn't (or isn't quite) a secret formulation. BDH made it following a publication by J. R. Baker (1941) A fluid for softening tissues embedded in paraffin wax. Quart. J. Microsc. Sci. 61:75. Bakers experiments led him to the composition: Glycerol 10 60% ethyl alcohol: 90 (parts by volume). A more recent paper is by Maria Wynnchuk (1992) in J. Histotechnol. 15(4): 321-323. She used a mixture called Horne's modification of Baker's solution: 95% ethanol 9520 ml Glycerol 320 ml Acetone 80 ml Liquid phenol 80 ml (I guess that the "liquid phenol" is an 80% solution in water, not solid phenol that has been melted by heating.) 10 litres of softening solution seems a lot to make up! I wonder how long it lasts for. Wynnchuk states that this mixture is better than Baker's original and that Mollifex, which contains a "phenol derivative" is just as good. Anything here for Messrs Sue, Grabbit and Runne? John A. Kiernan, Department of Anatomy & Cell Biology, The University of Western Ontario, LONDON, Canada N6A 5C1 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. Sent: Monday, October 20, 2008 10:42 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Baker's Fluid Does anyone have the recepe fpr Baker's fluid? Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: jo-ann.bader@mcgill.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From laurie.colbert <@t> huntingtonhospital.com Mon Oct 20 10:18:45 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Oct 20 10:18:54 2008 Subject: [Histonet] Full Time Histotech Needed Message-ID: <57BE698966D5C54EAE8612E8941D768303F54E97@EXCHANGE3.huntingtonhospital.com> Huntington Hospital in Pasadena, CA has a full time histotech opening for an experienced histotech (2+ years). The position is Monday-Friday, 5:30 am - 2:00 pm, with on-call every 5th Saturday. Experienced applicants may contact me at the number below. Laurie Colbert Huntington Hospital Pasadena, CA 91105 (626) 397-8620 From jo-ann.bader <@t> mcgill.ca Mon Oct 20 10:34:56 2008 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Mon Oct 20 10:35:52 2008 Subject: [Histonet] Baker's Fluid References: <5D64396A0D4A5346BEBC759022AAEAA50E3799@ITSSSXM01V6.one.ads.che.org> Message-ID: Thanks that is the one I am looking for! Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: jo-ann.bader@mcgill.ca -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Mon 10/20/2008 11:16 AM To: Jo-Ann Bader, Ms.; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Baker's Fluid This was in the archives. Hope it helps! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax On Wed, 24 May 2000, Barry Rittman wrote: > what you need is "Mollifex" sold by BDH You shouldn't use secret mixtures, but Mollifex isn't (or isn't quite) a secret formulation. BDH made it following a publication by J. R. Baker (1941) A fluid for softening tissues embedded in paraffin wax. Quart. J. Microsc. Sci. 61:75. Bakers experiments led him to the composition: Glycerol 10 60% ethyl alcohol: 90 (parts by volume). A more recent paper is by Maria Wynnchuk (1992) in J. Histotechnol. 15(4): 321-323. She used a mixture called Horne's modification of Baker's solution: 95% ethanol 9520 ml Glycerol 320 ml Acetone 80 ml Liquid phenol 80 ml (I guess that the "liquid phenol" is an 80% solution in water, not solid phenol that has been melted by heating.) 10 litres of softening solution seems a lot to make up! I wonder how long it lasts for. Wynnchuk states that this mixture is better than Baker's original and that Mollifex, which contains a "phenol derivative" is just as good. Anything here for Messrs Sue, Grabbit and Runne? John A. Kiernan, Department of Anatomy & Cell Biology, The University of Western Ontario, LONDON, Canada N6A 5C1 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. Sent: Monday, October 20, 2008 10:42 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Baker's Fluid Does anyone have the recepe fpr Baker's fluid? Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: jo-ann.bader@mcgill.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From talulahgosh <@t> gmail.com Mon Oct 20 10:59:06 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Oct 20 10:59:11 2008 Subject: [Histonet] the ubiquitous yet incredibly important timer Message-ID: We're in the market for new timers. We have been using this kindfrom Fisher, which is exactly what we need but they break very easily. What we like about these is -when the time runs out and the alarm beeps for one minute the sound stops but the timer continues to count up until you stop it -you DON'T enter the time by pushing the buttons a certain number of times (ie, push minute button 30 times for 30 minutes) -they run on one AAA battery -the time display is pretty big -they have a magnet on the back Emily -- i'll render services that you may reasonably require http://www.youtube.com/watch?v=t_at7lEGpjg From asmith <@t> mail.barry.edu Mon Oct 20 11:02:51 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Mon Oct 20 11:05:30 2008 Subject: [Histonet] B5 fixative In-Reply-To: <48FC5795.ACD8.0085.0@parkview.com> References: <48F85924.ACD8.0085.0@parkview.com> <48F85F02020000770000638A@gwmail4.harthosp.org> <48FC5795.ACD8.0085.0@parkview.com> Message-ID: B5, Schaudinn's, SUSA, Helly's, and Zenker's fixatives contain mercury (II) ions, which are VERY poisonous. Sewage bacteria convert mercury (II) to methyl mercury, which is very, very poisonous. (For a shocking example of mercury toxicity, see the photo essay MINAMATA by W. Eugene Smith.) When these fixatives are used, the fixative and the first washing must be given to a waste disposal company that can recycle it. B5 substitutes substitute a less poisonous ion, usually zinc (II), for mercury. These substitutes contain other things that are more poisonous than zinc. I store them as if they were purely the most poisonous component and give them to a waste disposal company twice a year. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Monday, October 20, 2008 10:04 AM To: Piero Nelva; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] B5 fixative For all of the listed B5 substitutes mentioned, how are they disposed of and are they significantly safer? >>> "Piero Nelva" 10/17/2008 17:59 >>> I agree. The Occ Health and SAfety representative is your best bet to consign the B5 to history. Piero Nelva Anatomical pathology Monash Medical Centre Australia ----- Original Message ----- From: "Richard Cartun" To: ; "Bryan Watson" Sent: Saturday, October 18, 2008 12:46 AM Subject: Re: [Histonet] B5 fixative If you are having a hard time with a pathologist regarding the use of B5, get your safety people involved. They will take care of it. We stopped using B5 a very long time ago. We use formalin for all our lymph nodes and bone marrows. No one should be using B5 and manufacturers should stop making it. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Bryan Watson" 10/17/08 9:21 AM >>> How many still use B5 fixative? And for those who do not, what do you use as a replacement? One of our pathologists is insistent on using B5, and we'd rather try to get away from it. Thanks, Bryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.8.1/1731 - Release Date: 10/17/2008 7:01 PM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gedinite <@t> gmail.com Mon Oct 20 12:22:22 2008 From: gedinite <@t> gmail.com (Richard Nelson) Date: Mon Oct 20 12:22:29 2008 Subject: [Histonet] free phone consultations Message-ID: Hello: The Lab Consultants are experts in lab operations and offer free phone consultations. If you have questions concerning your lab activities please contact us on our toll free line: 866-677-0003. We specialize in lab design, set up, operation and supply and can help you meet CLIA standards. Rick Nelson The Lab Consultants 866-677-0002 ext 2 http://www.thelabconsultants.com/ From stella_quan <@t> dnar.com Mon Oct 20 12:34:20 2008 From: stella_quan <@t> dnar.com (Stella Quan) Date: Mon Oct 20 12:40:05 2008 Subject: [Histonet] Job Posting (Boston) Message-ID: <49110CB9C6A2FD4B9E9AA5FBC6E9F0B902B2C062E1@MBX75.ad2.softcom.biz> As a growing personalized medicine/cancer diagnostics company, DNAR is seeking to hire: * A full-time Senior Scientist (Ph.D.) with extensive experiences in IHC assay development and commercialization * A full-time Histologist (BS/BA) with HTL (ASCP) Certification and IHC experiences Please contact : Stella Quan, Ph.D. DNAR Senior Director of Product Development 8 Saint Mary's Street, Suite 603 Boston, MA 02215 Stella_quan@dnar.com From carrolpb <@t> umdnj.edu Mon Oct 20 13:00:18 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Oct 20 13:00:41 2008 Subject: [Histonet] free phone consultations In-Reply-To: References: Message-ID: <48FCC732.1030202@umdnj.edu> Can The Lab Consultants use their technical expertise to not spam this list? Thanks! Richard Nelson wrote: > Hello: > > The Lab Consultants are experts in lab operations and offer free phone > consultations. If you have questions concerning your lab activities please > contact us on our toll free line: 866-677-0003. We specialize in lab > design, set up, operation and supply and can help you meet CLIA standards. > > Rick Nelson > The Lab Consultants > 866-677-0002 ext 2 > http://www.thelabconsultants.com/ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From kmerriam2003 <@t> yahoo.com Mon Oct 20 13:03:44 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Oct 20 13:03:48 2008 Subject: [Histonet] anti-Human CD68 Message-ID: <462762.46010.qm@web50309.mail.re2.yahoo.com> Hello, I am looking for a CD68 antibody that will work in Human FFPE tissues, but?it needs to be from a species other than mouse (preferably rabbit or goat).? Any recommendations? Thanks, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From thisisann <@t> aol.com Mon Oct 20 14:44:06 2008 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Mon Oct 20 14:44:26 2008 Subject: [Histonet] Medical Equipment Source Message-ID: <8CB010358B05862-BF4-886@WEBMAIL-DZ12.sysops.aol.com> Has anyone had any dealings with a company located in Mars, PA called Medical Equipment Source?? I visited their booth at the NSH and am comtemplating purchasing equipment (new and used) from them.? I was wondering if anyone has used them and, if so, what comments can you share about your experience. Annj From ngohad <@t> gmail.com Mon Oct 20 15:30:04 2008 From: ngohad <@t> gmail.com (Neeraj Gohad) Date: Mon Oct 20 15:30:09 2008 Subject: [Histonet] Microtome Knife Sharpner Message-ID: <48FCEA4C.1050802@gmail.com> Dear List, Our lab has American Optical Model 925 and 935 Microtome knife sharpeners and couple of microtome knives we want to sharpen. We need the fine and coarse abrasive solution that you need to sharpen the knives, does anybody know good source for these solution or an alternative? Vendors please feel free to contact me offline! Thanks, Raj. Neeraj V. Gohad, PhD Postdoctoral Fellow, Okeanos Research Group Department of Biological Sciences 132 Long Hall,Clemson University Clemson, SC-29634 864-656-3597 neerajg@clemson.edu From leiker <@t> buffalo.edu Mon Oct 20 15:36:25 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Mon Oct 20 15:36:32 2008 Subject: [Histonet] Medical Equipment Source In-Reply-To: <8CB010358B05862-BF4-886@WEBMAIL-DZ12.sysops.aol.com> References: <8CB010358B05862-BF4-886@WEBMAIL-DZ12.sysops.aol.com> Message-ID: <3A74ABF709AB2C1C01E3127F@bchwxp2702.ad.med.buffalo.edu> Hi Ann, Our lab purchased a Leica 2030 microtome (using disposable blades)through Medical Equipment Source. I'd looked at several companies that sell refurbished microtomes, and went with this company because of the cost ($3000) that included a warranty and a newer knife holder than the original one (at my request). They were also very pleasant to deal with. I talked to Luigi Mascio and he was good at keeping in touch by phone and email while the unit was being refurbished for us. We purchased the microtome back in May of this year (5 months ago) and have had no problems with it thus far - it works beautifully. Merced --On Monday, October 20, 2008 3:44 PM -0400 thisisann@aol.com wrote: > > Has anyone had any dealings with a company located in Mars, PA called > Medical Equipment Source?? I visited their booth at the NSH and am > comtemplating purchasing equipment (new and used) from them.? I was > wondering if anyone has used them and, if so, what comments can you share > about your experience. Annj > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 In order to put yourself in someone else's shoes, you must first take off yours. From jcox90 <@t> yahoo.com Mon Oct 20 15:47:23 2008 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Mon Oct 20 15:47:31 2008 Subject: Fw: Re: [Histonet] Medical Equipment Source Message-ID: <996333.57725.qm@web56803.mail.re3.yahoo.com> Hi Ann, I also just purchased a Leica XL Autostainer and Leica CV 5000 coverslipper recently from Medical Equipment Source?after calling all of the usual companies offering refurbished equipment. ?The pricing is a lot less than everyone else with?wonderful customer service, fast shipping?and? better warranty. Would highly recommend!! I have set up 5 labs now and have used them all, this one is definitely the winner!! Wish I would have known about them sooner!! And NO, I am not getting any kickbacks, LOL... Jill Cox HT (ASCP) ? ? ? ? --- On Mon, 10/20/08, Merced Leiker wrote: From: Merced Leiker Subject: Re: [Histonet] Medical Equipment Source To: thisisann@aol.com, histonet@lists.utsouthwestern.edu Date: Monday, October 20, 2008, 1:36 PM Hi Ann, Our lab purchased a Leica 2030 microtome (using disposable blades)through Medical Equipment Source. I'd looked at several companies that sell refurbished microtomes, and went with this company because of the cost ($3000) that included a warranty and a newer knife holder than the original one (at my request). They were also very pleasant to deal with. I talked to Luigi Mascio and he was good at keeping in touch by phone and email while the unit was being refurbished for us. We purchased the microtome back in May of this year (5 months ago) and have had no problems with it thus far - it works beautifully. Merced --On Monday, October 20, 2008 3:44 PM -0400 thisisann@aol.com wrote: > > Has anyone had any dealings with a company located in Mars, PA called > Medical Equipment Source?? I visited their booth at the NSH and am > comtemplating purchasing equipment (new and used) from them.? I was > wondering if anyone has used them and, if so, what comments can you share > about your experience. Annj > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 In order to put yourself in someone else's shoes, you must first take off yours. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Mon Oct 20 17:02:07 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Oct 20 17:02:19 2008 Subject: [Histonet] Acidified Hematoxylin In-Reply-To: Message-ID: You might haveto make some in-house. The following is easy to make and can be used soon after preparing. Use a good control (skin, appendix) to control your times: Debidin's Haematoxylin This solution uses sodium hypochlorite as the oxidant in the preparation of alum haematoxylin. This reagent also has the advantage of being a bacteriostat (Debidin 1991). Fixation: 10% buffered formalin. Microtomy: paraffin sections at 5?m. Reagents: Haematoxylin (CI 75290) 2.5g Potassium Alum 50g Distilled water 500ml 5.6% Sodium hypochlorite 2ml Glacial Acetic Acid 2.5ml Dissolve the Alum in distilled water with the aid of heat. Add the haematoxylin and mix. Cover and leave mixture at 60oC overnight. Add the sodium hypochlorite and allow to stand for one week, add acetic acid. Debidin (1991) Histologic 11(2):232. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. Sent: Tuesday, 21 October 2008 12:07 AM To: Histonet@lists.utsouthwestern.edu Cc: Melina Narlis, Ms Subject: [Histonet] Acidified Hematoxylin Good Morning Everyone, We are in dire straights We are coming to the end of out Acidified Harris Hematoxylin and I was just informed that the company that produces it will not start production until November. We will never make it. Does anyone know where I can get some? Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: jo-ann.bader@mcgill.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From saby_joseph_a <@t> yahoo.com Mon Oct 20 19:57:25 2008 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Mon Oct 20 19:57:29 2008 Subject: [Histonet] fixative for brains? Message-ID: <139962.14642.qm@web33807.mail.mud.yahoo.com> Jenee- I have almost always used 10% NBF for brain fixation.? We're talking rats, mice, guinea pigs, hamsters, pigs, dogs, primates, etc. The only exception to that was when?we?were having an issue?with the brains settling to the bottom of the fixation container and developing flat spots where it came to rest on the bottom.? We tried adding 37% formaldehyde to the 10% NBF to change the density of the solution to "float" the brains (at least enough to keep them off the bottom.? This worked beautifully, except that the brains now stretched during sectioning, and we ended up with many wrinkles in all directions.? Talk about the law of unintended consequences!? What a mess!? Needless to say, we went back to the old standby, but supported our brains dorsal side down in a gauze suspension.? Unless the brain is very large (in which case I would suggest slicing it and fixing the slices separately), I would sticking with 10% NBF. Joe Saby ----- Original Message ---- From: "Jenee.S.Odani@hawaii.gov" To: histonet@lists.utsouthwestern.edu Sent: Friday, October 17, 2008 2:21:27 PM Subject: [Histonet] fixative for brains? I'm trying to revise our laboratories SOPs. They currently are using 20% NBF to fix mammalian brains and alcohol-formalin to fix avian brains. In the past, I've used 10% NBF for all brains. Does anyone have any input on this? I'd like to keep things simple, if possible, and just use one fixative. Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Xorren <@t> aol.com Tue Oct 21 08:46:40 2008 From: Xorren <@t> aol.com (Xorren@aol.com) Date: Tue Oct 21 08:46:55 2008 Subject: [Histonet] Re: Histonet Digest, Vol 59, Issue 25 Message-ID: Hello Histonetters, Does anyone know of any histotechnologist positions or cytoprep positions in the NY area. I have the NY state license and tons of experience. Thanks for your help. You may reach me directly at Xorren@aol.com ************** New MapQuest Local shows what's happening at your destination. Dining, Movies, Events, News & more. Try it out (http://local.mapquest.com/?ncid=emlcntnew00000002) From CIngles <@t> uwhealth.org Tue Oct 21 09:27:03 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Oct 21 09:28:13 2008 Subject: [Histonet] fixative for brains? References: <139962.14642.qm@web33807.mail.mud.yahoo.com> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120189@uwhis-xchng3.uwhis.hosp.wisc.edu> Hey! Who you callin' "Fixative for brains"? Claire :) From mpence <@t> grhs.net Tue Oct 21 09:38:11 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Oct 21 09:38:21 2008 Subject: [Histonet] cyto prep Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3946@IS-E2K3.grhs.net> I know this is a histo liserv, but I also know hat many histo labs do cytology also. Has anyone using ThinPrep seen lubricate artifact on their slides that affects the cells from attaching to the slide, therefore making it look like an inadequate specimen. What have you done to dilute the lubricate out? Thanks for any help, Mike From ree3 <@t> leicester.ac.uk Tue Oct 21 10:43:34 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Oct 21 10:44:06 2008 Subject: [Histonet] AEC/MSDS Message-ID: <7722595275A4DD4FA225B92CDBF174A1744C1B91CE@EXC-MBX3.cfs.le.ac.uk> Anybody know where I can get access to (or send me?) the MSDS sheet for the chromagen aminoethylcarbazole, have tried the usual suspects but no luck to date. Many thanks Richard Edwards Leicester University U.K. From wilson_c <@t> ricerca.com Tue Oct 21 12:17:05 2008 From: wilson_c <@t> ricerca.com (Wilson, Carol) Date: Tue Oct 21 12:17:11 2008 Subject: [Histonet] RE: Medical Equipment Source In-Reply-To: <20081021170302.36FBD2FE5F@cuda.ricerca.com> References: <20081021170302.36FBD2FE5F@cuda.ricerca.com> Message-ID: <9D443EB9D0270143B5AAF190CB1A58A309746C6E@dogwood.ricerca.com> I have dealt with Luigi and Wendy Mascio for several years now, and have always been pleased with my purchases as well as their customer service. I would not hesitate in recommending Medical Equipment Source. Carol Wilson, HT(ASCP) Lead Technician Histology - Associate Scientist III Ricerca Biosciences, LLC From Jerry <@t> ralambusa.com Tue Oct 21 12:20:49 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Tue Oct 21 12:20:54 2008 Subject: [Histonet] RE: Medical Equipment Source (thisisann@aol.com) Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B5F2F@server.ralambusa.com> As a competitor on some levels and a parnter on others, I can say that Medical Equipment Source is a very honest and reputable company. I have never known them to make a promise they could not keep or surpass. I would highly recommend! ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ From cmiller <@t> physlab.com Tue Oct 21 12:22:52 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Oct 21 12:23:00 2008 Subject: [Histonet] Aspyra HELP!! Message-ID: <000701c933a1$a65661c0$3d02a8c0@plab.local> Does anyone out in histoland have the Aspyra -Cyberpath computer system?? We are currently setting ours up and I need some serious help. I prefer not to say it here so I wont be flamed by a representative of the company. PLEASE HELP! Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From c.m.vanderloos <@t> amc.uva.nl Tue Oct 21 12:54:02 2008 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Tue Oct 21 12:54:12 2008 Subject: [Histonet] RE: anti-Human CD68 Message-ID: Hi Kim,I wondering if your question for an anti-CD68 antibody raised in rabbit or goat is because of double staining plans. If so, please realize that the Dako antibody, clone PG-M1, is a mouse IgG3. That easily allows double staining with other mouse IgG1 or IgG2a antibodies. Go to Southern Biotechnology Associates (www. southernbiotech.com) for specific second step antibodies conjugated with alk. phos., peroxidase or biotin. Just a guess!Cheers,ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Mon, 20 Oct 2008 11:03:44 -0700 (PDT) From: Kim Merriam Subject: [Histonet] anti-Human CD68 To: Histonet histonet@lists.utsouthwestern.edu Hello, I am looking for a CD68 antibody that will work in Human FFPE tissues, but it needs to be from a species other than mouse (preferably rabbit or goat). Any recommendations? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From kmerriam2003 <@t> yahoo.com Tue Oct 21 13:05:29 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Oct 21 13:05:33 2008 Subject: [Histonet] Re: anti-Human CD68 Message-ID: <447280.25916.qm@web50312.mail.re2.yahoo.com> Hi Chris- Thanks for the insight, I never would have even thought?to check that the mouse isotypes were?different.? I will give it a look! Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: C.M. van der Loos To: histonet@lists.utsouthwestern.edu Cc: kmerriam2003@yahoo.com Sent: Tuesday, October 21, 2008 1:54:02 PM Subject: RE: anti-Human CD68 Hi Kim, I wondering if your?question for an anti-CD68 antibody?raised in rabbit or goat is because of double staining plans. If?so, please realize that the Dako antibody, clone PG-M1, is a mouse IgG3. That easily allows?double staining with other mouse IgG1 or IgG2a antibodies. Go to Southern Biotechnology Associates (www. southernbiotech.com) for specific second step antibodies conjugated with alk. phos., peroxidase or biotin. Just a guess! Cheers, Chris Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Mon, 20 Oct 2008 11:03:44 -0700 (PDT) From: Kim Merriam Subject: [Histonet] anti-Human CD68 To: Histonet histonet@lists.utsouthwestern.edu Hello, I am looking for a CD68 antibody that will work in Human FFPE tissues, but it needs to be from a species other than mouse (preferably rabbit or goat). Any recommendations? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From gvdobbin <@t> ihis.org Tue Oct 21 14:52:17 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Tue Oct 21 14:52:47 2008 Subject: [Histonet] C-erbB-2 SP3 clone Message-ID: Hello Folks, Is anyone out there using this clone from LabVision? I am trying to decide what volume to purchase in order to try the Ab out. There is a 0.1 mL size available and that would be my preference as long as the starting dilution is sufficiently high to allow several validation runs. So my question is what dilution range am I looking at for this clone? Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "And in the end it's not the years in your life that count. It's the life in your years." - Abraham Lincoln ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From hyters <@t> onid.orst.edu Tue Oct 21 17:50:40 2008 From: hyters <@t> onid.orst.edu (hyters@onid.orst.edu) Date: Tue Oct 21 17:50:44 2008 Subject: [Histonet] Medimachine Message-ID: <20081021155040.162314oukb29jgsg@webmail.oregonstate.edu> Hello, I am going to try out a Medimachine and was wondering if anybody out there had any experience with it. The plan is to take a tough tissue (skin) into a single suspension for use in FACS. Is there any protocols/troubleshooting that I need to know about? Thanks, Stephen From jvicidomini1 <@t> comcast.net Wed Oct 22 08:22:24 2008 From: jvicidomini1 <@t> comcast.net (Joanne) Date: Wed Oct 22 08:22:30 2008 Subject: [Histonet] Re: Histonet Digest, Vol 59, Issue 24 Message-ID: <10270EB4B7FC436C8C676523FC7B4311@JoannePC> you look wonderful . . . . .i'm off to see dr. pain. love, joanne ----- Original Message ----- From: To: Sent: Sunday, October 19, 2008 1:00 PM Subject: Histonet Digest, Vol 59, Issue 24 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Alcian Yellow (Sinoera Tech) > 2. RE: Re: Alcian Yellow (connie grubaugh) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 19 Oct 2008 15:35:18 +0800 > From: "Sinoera Tech" > Subject: [Histonet] Re: Alcian Yellow > To: "Blazek, Linda" , > , > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Dear Sir, > > We can supply you with Alcian Yellow and Alcian Blue 8GX. > > Kind Regards. > - > Minggeng Wang, Ph.D / President > SUZHOU SINOERA CHEM CO., LTD. > 125 Binhe Road > Suzhou New & Hi-Tech District > 215011 China > Fax: +86 512 68258994 > Tel: +86 512 68246939 > http://www.sinoeratech.com > > > > From: Blazek, Linda > Sent: Friday, September 22, 2006 10:12 PM > To: scott142@comcast.net ; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Alcian Yellow **HELP** > > > I'd also be interested! Want to share? Or if anyone knows of a good > substitute that info would be appreciated too. > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > scott142@comcast.net > Sent: Friday, September 22, 2006 9:48 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Alcian Yellow **HELP** > > I routinely run a procedure that works very well with old lots of Alcian > Yellow. Unfortunately I have no more lots of old Alcian Yellow. If > anyone has inventory of Alcian Yellow that they do not use, I would be > very eager to purchase it from you. > > Thanks you... > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 2 > Date: Sun, 19 Oct 2008 09:47:58 -0700 > From: connie grubaugh > Subject: RE: [Histonet] Re: Alcian Yellow > To: Sinoera Tech , "Blazek, Linda" > , , > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > American Master Tech has Alcian Yellow > > > Connie G. > >> From: billions1998@hotmail.com >> To: lblazek@digestivespecialists.com; scott142@comcast.net; >> histonet@lists.utsouthwestern.edu >> Date: Sun, 19 Oct 2008 15:35:18 +0800 >> CC: >> Subject: [Histonet] Re: Alcian Yellow >> >> >> Dear Sir, >> >> We can supply you with Alcian Yellow and Alcian Blue 8GX. >> >> Kind Regards. >> - >> Minggeng Wang, Ph.D / President >> SUZHOU SINOERA CHEM CO., LTD. >> 125 Binhe Road >> Suzhou New & Hi-Tech District >> 215011 China >> Fax: +86 512 68258994 >> Tel: +86 512 68246939 >> http://www.sinoeratech.com >> >> >> >> From: Blazek, Linda >> Sent: Friday, September 22, 2006 10:12 PM >> To: scott142@comcast.net ; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Alcian Yellow **HELP** >> >> >> I'd also be interested! Want to share? Or if anyone knows of a good >> substitute that info would be appreciated too. >> >> Linda Blazek HT (ASCP) >> Manager/Supervisor >> GI Pathology of Dayton >> 7415 Brandt Pike >> Huber Heights, OH 45424 >> Phone: (937) 293-4424 ext 7118 >> Email: lblazek@digestivespecialists.com >> >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> scott142@comcast.net >> Sent: Friday, September 22, 2006 9:48 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Alcian Yellow **HELP** >> >> I routinely run a procedure that works very well with old lots of Alcian >> Yellow. Unfortunately I have no more lots of old Alcian Yellow. If >> anyone has inventory of Alcian Yellow that they do not use, I would be >> very eager to purchase it from you. >> >> Thanks you... >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > You live life beyond your PC. So now Windows goes beyond your PC. > http://clk.atdmt.com/MRT/go/115298556/direct/01/ > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 59, Issue 24 > **************************************** From Katie.Sousa <@t> bhs.org Wed Oct 22 09:04:12 2008 From: Katie.Sousa <@t> bhs.org (Sousa, Katie) Date: Wed Oct 22 09:04:44 2008 Subject: [Histonet] RE: Histonet Digest, Vol 59, Issue 25 In-Reply-To: <3d8d8c0f-ee6b-42a1-bb76-1d241ea7adf7@bhsexchub01.bhs.org> References: <3d8d8c0f-ee6b-42a1-bb76-1d241ea7adf7@bhsexchub01.bhs.org> Message-ID: <3115ECE23B13C94CACDB17711212E7D868D1CB63A6@bhsexc01.bhs.org> Does anyone use agar in a procedure to prepare cell blocks from cytology material? I am interested in such a procedure as a method to help eliminate/reduce "floaters" in cell block preparations? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, October 20, 2008 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 59, Issue 25 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Question on Von Kossa (Patsy Ruegg) 2. Madison WI (Steven Coakley) 3. eosin pH (Hana Peter) 4. Elastin Aldehyde Fuchsin (Adam Boanas) 5. Fwd: [Histonet] Question on Von Kossa (Anne van Binsbergen) 6. Re: eosin pH (Rene J Buesa) 7. Acidified Hematoxylin (Jo-Ann Bader, Ms.) 8. Re: Elastin Aldehyde Fuchsin (renafail@bellsouth.net) 9. Re: B5 fixative (Bryan Watson) 10. getting rid of biotin-avidin background (Tyrone Genade) 11. help-fibrotic tissue (godsgalnow@aol.com) 12. Re: getting rid of biotin-avidin background (anh2006@med.cornell.edu) 13. Histology Openings in Seattle (Chris Handrahan) 14. Job Opening (Alice Bonaiuto) 15. Baker's Fluid (Jo-Ann Bader, Ms.) 16. RE: Baker's Fluid (Weems, Joyce) 17. Full Time Histotech Needed (Laurie Colbert) 18. RE: Baker's Fluid (Jo-Ann Bader, Ms.) 19. the ubiquitous yet incredibly important timer (Emily Sours) 20. RE: B5 fixative (Smith, Allen) ---------------------------------------------------------------------- Message: 1 Date: Sun, 19 Oct 2008 11:09:18 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] Question on Von Kossa To: "'Amos Brooks'" , , Message-ID: <0190942AC6114F07978BD3775EC76F49@ihctechq9h2qof> Content-Type: text/plain; charset="iso-8859-1" That is how I do VK, I put it in the window for 20 min or so. I do a special H&E counterstain on mine, using an aqueous eosin. The eosin will light up the osteoid by fluorescing under uv light. We use this with a image analysis system to measure total area, calcified bone area (light scope, from the black silver stain) osteoid seam thickness (fluorescent scope using the eosin fluorescent property, everything else will be dark except the osteoid seams) if you labeled the bone with a fluorchrome you can just look at an unstained section to measure area between two labels. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, October 17, 2008 10:15 PM To: Herrick.James@mayo.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Question on Von Kossa James, Try this: once the sections are brought to water, 5% silver nitrate in either bright sunlight or a 60-100 watt incandescent light bulb for 30 min (check for browning of the control tissue adjust time as needed). Rinse well in distilled water. 5% Sodium Thiosulfate for 1 min. Rinse and counterstain with nuclear fast red or whatever you think would look cool :-). Dehydrate, clear and coverslip the slides. Of course, as with any silver stain use acid cleaned glassware and gloves unless you like watching your fingers turn black in the sunlight. Really well decalcified tissue usually has the Calcium washed out. Try a calcified breast or something that wasn't decalcified. This causes microtomists to curse a lot, but it makes great VonKossa controls. Have fun, Amos Message: 10 Date: Thu, 16 Oct 2008 15:45:11 -0500 From: "Herrick, James L." Subject: [Histonet] Question on Von Kossa To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello everyone, Does anyone have a good Von Kossa stain protocol that they would not mind sharing, on animal bone tissue (femur/tibia) that has been embedded in GMA or MMA (sections are between 5 and 10 ?m thick)? I would appreciate it greatly. Thank you much. Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Sun, 19 Oct 2008 13:56:30 -0700 (PDT) From: Steven Coakley Subject: [Histonet] Madison WI To: Histonet@lists.utsouthwestern.edu Message-ID: <160679.26224.qm@web38205.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii HT(ASCP) 14 years experience looking for employment in Madison WI. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 3 Date: Mon, 20 Oct 2008 10:29:54 +0200 From: Hana Peter Subject: [Histonet] eosin pH To: histonet@lists.utsouthwestern.edu Message-ID: <48FC4182.8050507@gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi! Can someone tell me the right pH for eosin y 1% aqueous solution? The one I made from powder has pH 6.3. Is this OK? The commercial one we usually buy is a bit lower (5.7). Thank you in advance! Hana Peter ------------------------------ Message: 4 Date: Mon, 20 Oct 2008 11:07:56 +0100 From: "Adam Boanas" Subject: [Histonet] Elastin Aldehyde Fuchsin To: Message-ID: <22ADCE0141FB0145A580E85CE3C70BD6B5690B@ex2003.winserv.renovo-ltd.com> Content-Type: text/plain; charset=WINDOWS-1252 Hello, I have been using Gomori`s Aldehyde Fuchsin to stain human skin for elastin. The stain works fine and is clear and specific. I am using light green as a counterstain. My problem is that once stained, the slides have an excess granular purple "dotted" appearance upon the entire surface of the slide. This is not limited to the tissue position upon the slide, but across the clear glass too. We have tried using both superfrost plus charged slides and uncharged slides but the problem still is present. This precipitate is spoiling an otherwise great stain. Does anyone have any idea about what this is and how it could be prevented? (Our next guess would be to re-filter the fuchsin solution prior to use every time) Many thanks Adam Adam Boanas Histology Manager Renovo Ltd PLEASE NOTE OUR TELEPHONE NUMBERS ARE CHANGING -PLEASE AMEND YOUR RECORDS: >From Monday 07/06/2008 our Telephone number will be as follows: Switchboard: 0161 276 7100 extension 7716 LEGAL NOTICE This e-mail is from Renovo Group plc (Registered No 5427608 England). The information contained in this e-mail and any files transmitted with it are confidential, may be privileged and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient any disclosure, copying, retransmission, distribution, dissemination, action taken or omitted to be taken in reliance upon this e-mail is strictly prohibited and may be unlawful. If you have received this communication in error please notify the sender by e-mail or by telephone on +44(0)161 276 7100, delete it from your system and destroy any copies of it. Please note that e-mails from Renovo Group plc pass through a virus checker but e-mails can be intercepted or affected by different viruses. Neither Renovo Group plc nor the sender accepts any liability or responsibility for viruses or other material introduced with this message and the recipient should ensure that the e-mail and attachments (if any) are actually virus free. Any views expressed by an individual within this e-mail do not necessarily represent the views of Renovo Group plc. No contract may be concluded on behalf of Renovo Group plc by means of e-mail communications. Renovo Group plc, Manchester Incubator Building, 48 Grafton Street, Manchester, M13 9XX, UK Tel: +44(0)161 276 7100 Fax: +44(0)161 276 7200 Website: http://www.renovo.com ------------------------------ Message: 5 Date: Mon, 20 Oct 2008 16:45:23 +0400 From: "Anne van Binsbergen" Subject: Fwd: [Histonet] Question on Von Kossa To: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 no - thats the really cool thing - think about it... its that part of the bone which is usually quite hard but softens with stewing/cooking and could be bitten off and chewed (if desired) no demineralisation has taken place yet it is soft enough to slice with a blade and once processed it cuts like butter on the microtome i also have an amazing source of GMS control for fungus - an old mildewed orange!!! same process applies - slice off a piece, process and embed - stains on PAS and GMS!!! quite stunning a pal of mine says that fish liver makes and amazing Oil red 'O' QC but have not yet tried that one i also hunt for liver QC material here in a country which does not do autopsies - we trot off to the local butcher with a small jar of formalin and pop a small slice of fresh beef liver into the formalin - am sure that other liver would work just as wekk some may argue that it is animal tissue but in my opinion it it not the tissue but the glycogen we need to demo - so its not an issue - my Paths are happy so im happy 2008/10/20 Patsy Ruegg Do you have to decal it? > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech > > 12635 Montview Blvd. #215 > > Aurora, CO 80045 > > 720-859-4060 > > fax 720-859-4110 > > pruegg@ihctech.net > > www.ihctech.net > > www.ihcrg.org > > > > > ------------------------------ > > *From:* Anne van Binsbergen [mailto:annigyg@gmail.com] > *Sent:* Sunday, October 19, 2008 9:48 PM > *To:* Patsy Ruegg > *Subject:* Re: [Histonet] Question on Von Kossa > > > > for an excellent VK control try this: > > > > after you have eaten all the meat from the bones of a chicken stew - keep > the thigh bones. > > Cut off the soft part on the end, pop into a casette, process as usual and > viola!!! > > Try it for yourself - works like a charm > > ;)) > > Annie > > > > > > > > 2008/10/19 Patsy Ruegg > > That is how I do VK, I put it in the window for 20 min or so. I do a > special H&E counterstain on mine, using an aqueous eosin. The eosin will > light up the osteoid by fluorescing under uv light. We use this with a > image analysis system to measure total area, calcified bone area (light > scope, from the black silver stain) osteoid seam thickness (fluorescent > scope using the eosin fluorescent property, everything else will be dark > except the osteoid seams) if you labeled the bone with a fluorchrome you > can > just look at an unstained section to measure area between two labels. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos > Brooks > Sent: Friday, October 17, 2008 10:15 PM > To: Herrick.James@mayo.edu; histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question on Von Kossa > > James, > Try this: once the sections are brought to water, 5% silver nitrate in > either bright sunlight or a 60-100 watt incandescent light bulb for 30 min > (check for browning of the control tissue adjust time as needed). Rinse > well > in distilled water. 5% Sodium Thiosulfate for 1 min. Rinse and counterstain > with nuclear fast red or whatever you think would look cool :-). Dehydrate, > clear and coverslip the slides. > Of course, as with any silver stain use acid cleaned glassware and gloves > unless you like watching your fingers turn black in the sunlight. Really > well decalcified tissue usually has the Calcium washed out. Try a calcified > breast or something that wasn't decalcified. This causes microtomists to > curse a lot, but it makes great VonKossa controls. > > Have fun, > Amos > > Message: 10 > Date: Thu, 16 Oct 2008 15:45:11 -0500 > From: "Herrick, James L." > Subject: [Histonet] Question on Von Kossa > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Hello everyone, > > Does anyone have a good Von Kossa stain protocol that they would not mind > sharing, on animal bone tissue (femur/tibia) that has been embedded in GMA > or MMA (sections are between 5 and 10 ?m thick)? I would appreciate it > greatly. Thank you much. > > Jim > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > -- > Anne van Binsbergen (Hope) > Abu Dhabi > UAE > -- Anne van Binsbergen (Hope) Abu Dhabi UAE -- Anne van Binsbergen (Hope) Abu Dhabi UAE ------------------------------ Message: 6 Date: Mon, 20 Oct 2008 05:59:45 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] eosin pH To: histonet@lists.utsouthwestern.edu, Hana Peter Message-ID: <478046.83469.qm@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The eosin should be acidic and you get to that point adding 1% aq. sol. of acetic acid to you prepared solution, at a rate of 1 mL acetic/100 mL of eosin solution. Ren? J. --- On Mon, 10/20/08, Hana Peter wrote: From: Hana Peter Subject: [Histonet] eosin pH To: histonet@lists.utsouthwestern.edu Date: Monday, October 20, 2008, 4:29 AM Hi! Can someone tell me the right pH for eosin y 1% aqueous solution? The one I made from powder has pH 6.3. Is this OK? The commercial one we usually buy is a bit lower (5.7). Thank you in advance! Hana Peter _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 7 Date: Mon, 20 Oct 2008 09:07:24 -0400 From: "Jo-Ann Bader, Ms." Subject: [Histonet] Acidified Hematoxylin To: Cc: "Melina Narlis, Ms" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Good Morning Everyone, We are in dire straights We are coming to the end of out Acidified Harris Hematoxylin and I was just informed that the company that produces it will not start production until November. We will never make it. Does anyone know where I can get some? Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: jo-ann.bader@mcgill.ca ------------------------------ Message: 8 Date: Mon, 20 Oct 2008 14:02:13 +0000 From: renafail@bellsouth.net Subject: Re: [Histonet] Elastin Aldehyde Fuchsin To: "Adam Boanas" , Message-ID: <102020081402.11584.48FC8F65000436C100002D4022243651029B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net> Content-Type: text/plain The solution does need to be filtered before each use Rena Fail -------------- Original message from "Adam Boanas" : -------------- > Hello, > > > > I have been using Gomori`s Aldehyde Fuchsin to stain human skin for > elastin. The stain works fine and is clear and specific. I am using > light green as a counterstain. My problem is that once stained, the > slides have an excess granular purple "dotted" appearance upon the > entire surface of the slide. This is not limited to the tissue position > upon the slide, but across the clear glass too. We have tried using both > superfrost plus charged slides and uncharged slides but the problem > still is present. This precipitate is spoiling an otherwise great stain. > Does anyone have any idea about what this is and how it could be > prevented? (Our next guess would be to re-filter the fuchsin solution > prior to use every time) > > > > Many thanks > > Adam > > > > Adam Boanas > Histology Manager > Renovo Ltd > > PLEASE NOTE OUR TELEPHONE NUMBERS ARE CHANGING -PLEASE AMEND YOUR > RECORDS: > > >From Monday 07/06/2008 our Telephone number will be as follows: > > Switchboard: 0161 276 7100 extension 7716 > > > > LEGAL NOTICE > This e-mail is from Renovo Group plc (Registered No 5427608 England). The > information contained in this e-mail and any files transmitted with > it are confidential, may be privileged and are intended solely for the use of > the individual or entity to whom they are addressed. If you are > not the intended recipient any disclosure, copying, retransmission, > distribution, dissemination, action taken or omitted to be taken in reliance > upon this e-mail is strictly prohibited and may be unlawful. If you have > received this communication in error please notify the sender by e-mail > or by telephone on +44(0)161 276 7100, delete it from your system and destroy > any copies of it. > Please note that e-mails from Renovo Group plc pass through a virus checker but > e-mails can be intercepted or affected by different viruses. > Neither Renovo Group plc nor the sender accepts any liability or responsibility > for viruses or other material introduced with this message and > the recipient should ensure that the e-mail and attachments (if any) are > actually virus free. > Any views expressed by an individual within this e-mail do not necessarily > represent the views of Renovo Group plc. No contract may be concluded > on behalf of Renovo Group plc by means of e-mail communications. > Renovo Group plc, Manchester Incubator Building, 48 Grafton Street, Manchester, > M13 9XX, UK > Tel: +44(0)161 276 7100 Fax: +44(0)161 276 7200 > Website: http://www.renovo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 20 Oct 2008 10:04:05 -0400 From: "Bryan Watson" Subject: Re: [Histonet] B5 fixative To: "Piero Nelva" , Message-ID: <48FC5795.ACD8.0085.0@parkview.com> Content-Type: text/plain; charset=US-ASCII For all of the listed B5 substitutes mentioned, how are they disposed of and are they significantly safer? >>> "Piero Nelva" 10/17/2008 17:59 >>> I agree. The Occ Health and SAfety representative is your best bet to consign the B5 to history. Piero Nelva Anatomical pathology Monash Medical Centre Australia ----- Original Message ----- From: "Richard Cartun" To: ; "Bryan Watson" Sent: Saturday, October 18, 2008 12:46 AM Subject: Re: [Histonet] B5 fixative If you are having a hard time with a pathologist regarding the use of B5, get your safety people involved. They will take care of it. We stopped using B5 a very long time ago. We use formalin for all our lymph nodes and bone marrows. No one should be using B5 and manufacturers should stop making it. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Bryan Watson" 10/17/08 9:21 AM >>> How many still use B5 fixative? And for those who do not, what do you use as a replacement? One of our pathologists is insistent on using B5, and we'd rather try to get away from it. Thanks, Bryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.8.1/1731 - Release Date: 10/17/2008 7:01 PM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 20 Oct 2008 16:07:07 +0200 From: "Tyrone Genade" Subject: [Histonet] getting rid of biotin-avidin background To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi all, I have a problem and think I have figured out a solution to it but need some expert advise. I'm using biotinylated GS isolectin to stain frozen brain sections for microglia. The GS isolectin is working very well but the avadin-fluourophore is binding to the endogenous biotin in the tissue causing a lot of back ground. I'm thinking of fist blocking the sections by incubating it with unlabled avidin and then following it with another blocking step with unlabled biotin so that all the endogenous biotin is bound to avidin and the un-endogenous biotin bound sites of the avidin are then bound to unlabled biotin. This would then be followed by the GS Isolectin incubation etc... Can anyone suggest a reasonable starting concentration for the first biotin and avidin blocking steps? Do you think this will work at all? Kind regards -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@freeshell.org tel: +27-84-632-1925 (c) ******************************************************************************** "For there is one God, and there is one mediator between God and men, the man Christ Jesus, who gave himself as a ransom for all." 1 Timothy 2:5-6 ------------------------------ Message: 11 Date: Mon, 20 Oct 2008 10:14:04 -0400 From: godsgalnow@aol.com Subject: [Histonet] help-fibrotic tissue To: histonet@lists.utsouthwestern.edu Message-ID: <8CB00D53E12934B-784-2540@MBLK-M27.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" I need to finally call on the experts.? I have tried all of the tricks up my sleeve to cut extremely fibrotic Cerivical tissue.? It is not calcified, just fibrotic...it is like cutting glass. Help. Roxanne ------------------------------ Message: 12 Date: Mon, 20 Oct 2008 14:13:18 +0000 From: anh2006@med.cornell.edu Subject: Re: [Histonet] getting rid of biotin-avidin background To: tgenade@freeshell.org, histonet@lists.utsouthwestern.edu Message-ID: <861309140-1224512133-cardhu_decombobulator_blackberry.rim.net-1377979103-@bxe270.bisx.prod.on.blackberry> Content-Type: text/plain Why not use an avidin-biotin blocking kit (like those from Vector or Dako for example). They work well, are optiimized already and aren't that expensive. -----Original Message----- From: Tyrone Genade Date: Mon, 20 Oct 2008 16:07:07 To: Subject: [Histonet] getting rid of biotin-avidin background Hi all, I have a problem and think I have figured out a solution to it but need some expert advise. I'm using biotinylated GS isolectin to stain frozen brain sections for microglia. The GS isolectin is working very well but the avadin-fluourophore is binding to the endogenous biotin in the tissue causing a lot of back ground. I'm thinking of fist blocking the sections by incubating it with unlabled avidin and then following it with another blocking step with unlabled biotin so that all the endogenous biotin is bound to avidin and the un-endogenous biotin bound sites of the avidin are then bound to unlabled biotin. This would then be followed by the GS Isolectin incubation etc... Can anyone suggest a reasonable starting concentration for the first biotin and avidin blocking steps? Do you think this will work at all? Kind regards -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@freeshell.org tel: +27-84-632-1925 (c) ******************************************************************************** "For there is one God, and there is one mediator between God and men, the man Christ Jesus, who gave himself as a ransom for all." 1 Timothy 2:5-6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 20 Oct 2008 10:16:54 -0400 From: "Chris Handrahan" Subject: [Histonet] Histology Openings in Seattle To: Message-ID: Content-Type: text/plain; charset="us-ascii" Looking for ASCP certified Histotechs for the following openings day position, 7-3:30pm 2nd shift position, 6pm - 2:30am - pays $3 an hour shift differential 3rd shift position, 11pm - 7:30am - pays $10 an hour shift differential Pathologists' Assistant Lead- 10am-6:30pm, M-F IHC Lead - day position, at least 5+ yrs experience including 2 yrs in a lead role. These are all full time permanent positions. These are immediate needs so please contact me today if you are interested! Chris Handrahan Managing Director of Allied Health Healthcare Scouts 800-708-0605 office 321-231-5427 cell chrish@healthcarescouts.com www.healthcarescouts.com ------------------------------ Message: 14 Date: Mon, 20 Oct 2008 10:24:01 -0400 From: "Alice Bonaiuto" Subject: [Histonet] Job Opening To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Histology job opening for routine bench work &/ or IHC at Sarsota Pathology/Sarasota, Fl. For more information go to www.sarapath.com . Fax resumes to :(941) 362-8992 or HR@sarapath.com Thanks, Alice Bonaiuto, IHC Technical Specialist ------------------------------ Message: 15 Date: Mon, 20 Oct 2008 10:41:41 -0400 From: "Jo-Ann Bader, Ms." Subject: [Histonet] Baker's Fluid To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Does anyone have the recepe fpr Baker's fluid? Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: jo-ann.bader@mcgill.ca ------------------------------ Message: 16 Date: Mon, 20 Oct 2008 11:16:59 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Baker's Fluid To: "Jo-Ann Bader, Ms." , Message-ID: <5D64396A0D4A5346BEBC759022AAEAA50E3799@ITSSSXM01V6.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" This was in the archives. Hope it helps! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax On Wed, 24 May 2000, Barry Rittman wrote: > what you need is "Mollifex" sold by BDH You shouldn't use secret mixtures, but Mollifex isn't (or isn't quite) a secret formulation. BDH made it following a publication by J. R. Baker (1941) A fluid for softening tissues embedded in paraffin wax. Quart. J. Microsc. Sci. 61:75. Bakers experiments led him to the composition: Glycerol 10 60% ethyl alcohol: 90 (parts by volume). A more recent paper is by Maria Wynnchuk (1992) in J. Histotechnol. 15(4): 321-323. She used a mixture called Horne's modification of Baker's solution: 95% ethanol 9520 ml Glycerol 320 ml Acetone 80 ml Liquid phenol 80 ml (I guess that the "liquid phenol" is an 80% solution in water, not solid phenol that has been melted by heating.) 10 litres of softening solution seems a lot to make up! I wonder how long it lasts for. Wynnchuk states that this mixture is better than Baker's original and that Mollifex, which contains a "phenol derivative" is just as good. Anything here for Messrs Sue, Grabbit and Runne? John A. Kiernan, Department of Anatomy & Cell Biology, The University of Western Ontario, LONDON, Canada N6A 5C1 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. Sent: Monday, October 20, 2008 10:42 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Baker's Fluid Does anyone have the recepe fpr Baker's fluid? Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: jo-ann.bader@mcgill.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 17 Date: Mon, 20 Oct 2008 08:18:45 -0700 From: "Laurie Colbert" Subject: [Histonet] Full Time Histotech Needed To: Message-ID: <57BE698966D5C54EAE8612E8941D768303F54E97@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" Huntington Hospital in Pasadena, CA has a full time histotech opening for an experienced histotech (2+ years). The position is Monday-Friday, 5:30 am - 2:00 pm, with on-call every 5th Saturday. Experienced applicants may contact me at the number below. Laurie Colbert Huntington Hospital Pasadena, CA 91105 (626) 397-8620 ------------------------------ Message: 18 Date: Mon, 20 Oct 2008 11:34:56 -0400 From: "Jo-Ann Bader, Ms." Subject: RE: [Histonet] Baker's Fluid To: "Weems, Joyce" Cc: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Thanks that is the one I am looking for! Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: jo-ann.bader@mcgill.ca -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Mon 10/20/2008 11:16 AM To: Jo-Ann Bader, Ms.; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Baker's Fluid This was in the archives. Hope it helps! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax On Wed, 24 May 2000, Barry Rittman wrote: > what you need is "Mollifex" sold by BDH You shouldn't use secret mixtures, but Mollifex isn't (or isn't quite) a secret formulation. BDH made it following a publication by J. R. Baker (1941) A fluid for softening tissues embedded in paraffin wax. Quart. J. Microsc. Sci. 61:75. Bakers experiments led him to the composition: Glycerol 10 60% ethyl alcohol: 90 (parts by volume). A more recent paper is by Maria Wynnchuk (1992) in J. Histotechnol. 15(4): 321-323. She used a mixture called Horne's modification of Baker's solution: 95% ethanol 9520 ml Glycerol 320 ml Acetone 80 ml Liquid phenol 80 ml (I guess that the "liquid phenol" is an 80% solution in water, not solid phenol that has been melted by heating.) 10 litres of softening solution seems a lot to make up! I wonder how long it lasts for. Wynnchuk states that this mixture is better than Baker's original and that Mollifex, which contains a "phenol derivative" is just as good. Anything here for Messrs Sue, Grabbit and Runne? John A. Kiernan, Department of Anatomy & Cell Biology, The University of Western Ontario, LONDON, Canada N6A 5C1 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. Sent: Monday, October 20, 2008 10:42 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Baker's Fluid Does anyone have the recepe fpr Baker's fluid? Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: jo-ann.bader@mcgill.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 19 Date: Mon, 20 Oct 2008 11:59:06 -0400 From: "Emily Sours" Subject: [Histonet] the ubiquitous yet incredibly important timer To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 We're in the market for new timers. We have been using this kindfrom Fisher, which is exactly what we need but they break very easily. What we like about these is -when the time runs out and the alarm beeps for one minute the sound stops but the timer continues to count up until you stop it -you DON'T enter the time by pushing the buttons a certain number of times (ie, push minute button 30 times for 30 minutes) -they run on one AAA battery -the time display is pretty big -they have a magnet on the back Emily -- i'll render services that you may reasonably require http://www.youtube.com/watch?v=t_at7lEGpjg ------------------------------ Message: 20 Date: Mon, 20 Oct 2008 12:02:51 -0400 From: "Smith, Allen" Subject: RE: [Histonet] B5 fixative To: 'Bryan Watson' Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" B5, Schaudinn's, SUSA, Helly's, and Zenker's fixatives contain mercury (II) ions, which are VERY poisonous. Sewage bacteria convert mercury (II) to methyl mercury, which is very, very poisonous. (For a shocking example of mercury toxicity, see the photo essay MINAMATA by W. Eugene Smith.) When these fixatives are used, the fixative and the first washing must be given to a waste disposal company that can recycle it. B5 substitutes substitute a less poisonous ion, usually zinc (II), for mercury. These substitutes contain other things that are more poisonous than zinc. I store them as if they were purely the most poisonous component and give them to a waste disposal company twice a year. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Monday, October 20, 2008 10:04 AM To: Piero Nelva; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] B5 fixative For all of the listed B5 substitutes mentioned, how are they disposed of and are they significantly safer? >>> "Piero Nelva" 10/17/2008 17:59 >>> I agree. The Occ Health and SAfety representative is your best bet to consign the B5 to history. Piero Nelva Anatomical pathology Monash Medical Centre Australia ----- Original Message ----- From: "Richard Cartun" To: ; "Bryan Watson" Sent: Saturday, October 18, 2008 12:46 AM Subject: Re: [Histonet] B5 fixative If you are having a hard time with a pathologist regarding the use of B5, get your safety people involved. They will take care of it. We stopped using B5 a very long time ago. We use formalin for all our lymph nodes and bone marrows. No one should be using B5 and manufacturers should stop making it. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Bryan Watson" 10/17/08 9:21 AM >>> How many still use B5 fixative? And for those who do not, what do you use as a replacement? One of our pathologists is insistent on using B5, and we'd rather try to get away from it. Thanks, Bryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.8.1/1731 - Release Date: 10/17/2008 7:01 PM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 59, Issue 25 **************************************** ----------------------------------------- CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at (413) 794-0000 and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet web site at http://www.baystatehealth.com. From rclouse <@t> wellspan.org Wed Oct 22 10:47:53 2008 From: rclouse <@t> wellspan.org (Clouse, Rosanna) Date: Wed Oct 22 10:51:31 2008 Subject: [Histonet] Question about Leica Autostainer XL Message-ID: I have a question about a Leica Autostainer XL. We are getting the most peculiar odor from it during staining and none of us can quite describe it. One of our histotechs is very sensitive to it however. We have changed the filters and the odor is still there. Also, the arm mechanism is very warm--almost hot--and we were wondering if this is normal for operation. Thanks so much for any and all comments. Rosanna S. Clouse, SCT(ASCP) Division Manager, Cytology Gettysburg Hospital-Wellspan 147 Gettys Street Gettysburg, PA 17325 Phone: 717-337-4120 (x75134) Fax: 717-337-4236 rclouse@wellspan.org CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From jqb7 <@t> cdc.gov Wed Oct 22 10:56:20 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Oct 22 10:56:38 2008 Subject: [Histonet] Question about Leica Autostainer XL In-Reply-To: References: Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C39D@LTA3VS011.ees.hhs.gov> I would call my service tech. with your Leica distributor. Ours has never had a smell and the arm is not overly warm. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clouse, Rosanna Sent: Wednesday, October 22, 2008 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about Leica Autostainer XL I have a question about a Leica Autostainer XL. We are getting the most peculiar odor from it during staining and none of us can quite describe it. One of our histotechs is very sensitive to it however. We have changed the filters and the odor is still there. Also, the arm mechanism is very warm--almost hot--and we were wondering if this is normal for operation. Thanks so much for any and all comments. Rosanna S. Clouse, SCT(ASCP) Division Manager, Cytology Gettysburg Hospital-Wellspan 147 Gettys Street Gettysburg, PA 17325 Phone: 717-337-4120 (x75134) Fax: 717-337-4236 rclouse@wellspan.org CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDeMatteo <@t> srhs.org Wed Oct 22 10:56:16 2008 From: GDeMatteo <@t> srhs.org (DeMatteo, Gabriela) Date: Wed Oct 22 10:58:32 2008 Subject: FW: [Histonet] Question about Leica Autostainer XL References: Message-ID: No, it is not normal for the arm to get hot. You probably need your stainer checked. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Clouse, Rosanna Sent: Wed 10/22/2008 11:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about Leica Autostainer XL I have a question about a Leica Autostainer XL. We are getting the most peculiar odor from it during staining and none of us can quite describe it. One of our histotechs is very sensitive to it however. We have changed the filters and the odor is still there. Also, the arm mechanism is very warm--almost hot--and we were wondering if this is normal for operation. Thanks so much for any and all comments. Rosanna S. Clouse, SCT(ASCP) Division Manager, Cytology Gettysburg Hospital-Wellspan 147 Gettys Street Gettysburg, PA 17325 Phone: 717-337-4120 (x75134) Fax: 717-337-4236 rclouse@wellspan.org CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Wed Oct 22 11:28:24 2008 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Oct 22 11:28:31 2008 Subject: [Histonet] HIER Message-ID: We have been using the microwave HIER and have had good results, however when our microwave bits the dust I would like to have a pressure cooker method in place. I feel the pressure cooker is more consistent for all the slides. We are a veterinary diagnostic lab and I would like to have some idea of where to begin. I have looked at different protocols and they often indicate HIER in a pressure cooker but do not give the details. I currently use citrate buffer pH 6. I put the slides in refrigerated buffer and microwave on high for 1 min 45 sec. or until the buffer just starts to boil. I then set the microwave on 10% power for 10 minutes. Afterward the slides are allowed to cool in the microwave for 1 hour. We have a biocare Decloaker Chamber and I would appreciate help with the program I should use. Do you start with cold buffer or should I prewarm it? What temperature should I use? How long should I maintain the temperature? How long should it be before I remove the slides? I also am working with a new protocol that calls for heating in a steamer. Should the temperature of the buffer be warm, cold or room temp when I start? Thank you. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From Susan.Weber2 <@t> va.gov Wed Oct 22 10:59:52 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Wed Oct 22 11:47:44 2008 Subject: [Histonet] Question about Leica Autostainer XL In-Reply-To: References: Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76E6F@VHAV10MSGA1.v10.med.va.gov> Have you removed all the reagent containers? Even the water containers? We have noticed that without regular flushing of machine with a weak bleach solution (or disinfectant) we have mold growth which can be the cause of odor in the equipment. Not the reagent containers, the actual chamber interior. I had not noticed any extreme warmth coming from the arm mechanism,that is something you can ask the Leica rep or service rep. I have always found our rep and the general service of Leica to be very helpful and responsive. They may be able to pinpoint the source of the odor also. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clouse, Rosanna Sent: Wednesday, October 22, 2008 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about Leica Autostainer XL I have a question about a Leica Autostainer XL. We are getting the most peculiar odor from it during staining and none of us can quite describe it. One of our histotechs is very sensitive to it however. We have changed the filters and the odor is still there. Also, the arm mechanism is very warm--almost hot--and we were wondering if this is normal for operation. Thanks so much for any and all comments. Rosanna S. Clouse, SCT(ASCP) Division Manager, Cytology Gettysburg Hospital-Wellspan 147 Gettys Street Gettysburg, PA 17325 Phone: 717-337-4120 (x75134) Fax: 717-337-4236 rclouse@wellspan.org CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Wed Oct 22 12:55:07 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Oct 22 12:55:13 2008 Subject: [Histonet] Re: [IHCRG] HIER Message-ID: <676976.92977.qm@web50310.mail.re2.yahoo.com> In my experience, you can use the Biocare Decloaker with their default program for most protocols (I believe it heats?@ 125C for 10 seconds and then 90C for 10 minutes, but dont?quote me on that because I am not sure).? I do not preheat my buffer when I use the pressure cooker, since it heats up pretty quickly.? I have used a lower-temp protocol for some phospho-proteins (90C for 30 minutes), but that is the exception and not the rule. I also use a Black & Decker steamer on a regular basis.? I always preheat the buffer?(in the microwave) before putting?it in the steamer, because the steamer heats slowly and does not get to temps that are as high as the pressure cooker (I?check the steamer regularly and it gets to about 100C or so on most days).? I?usually steam the slides for 45-60?minutes. In the case of both heating systems, I usually let the slides cool for about 10 minutes and then rinse them in running water.? I have heard of other people cooling their slides for up to an hour and still others that do not cool at all.??I am not sure that how long you cool the slides really has an impact on anything (IMHO, it is all about the heating and not the cooling). As far as pH is concerned, that a whole other topic.??For most antibodies, a routine citrate or EDTA buffer works fine (pH 6-8 or so), but I have used the high-pH buffers on occasion (usually for nuclear proteins). I work in a biotech/ discovery research lab, so my particular lab does not need to follow strick regulatory guidelines (although I try to run the lab?in a "GLP-like" fashion). Just my 2-cents. Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Perry, Margaret" To: "ihcrg@googlegroups.com" ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, October 22, 2008 12:28:24 PM Subject: [IHCRG] HIER We have been using the microwave HIER and have had good results, however when our microwave bits the dust I would like to have a pressure cooker method in place. ?I feel the pressure cooker is more consistent for all the slides.? We are a veterinary diagnostic lab and I would like to have some idea of where to begin.? I have looked at different protocols and they often indicate HIER in a pressure cooker but do not give the details.? I currently use citrate buffer pH 6.? I put the slides in refrigerated buffer and microwave on high for 1 min 45 sec. or until the buffer just starts to boil.? I then set the microwave on 10% power for 10 minutes.? Afterward the slides are allowed to cool in the microwave for 1 hour. ?We have a biocare Decloaker Chamber and I would appreciate help with the program I should use. ?Do you start with cold buffer or should I prewarm it? ?What temperature should I use? How long should I maintain the temperature?? How long should it be before I remove the slides? ? I also am working with a new protocol that calls for heating in a steamer. ?Should the temperature of the buffer be warm, cold or room temp when I start? ? Thank you. ? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South DakotaState University Box 2175 North Campus Drive BrookingsSD 57007 ? --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- From shive003 <@t> umn.edu Wed Oct 22 13:23:05 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Oct 22 13:23:10 2008 Subject: [Histonet] HIER References: Message-ID: In my opinion/experience: The upside of using a microwave for HIER is that you can remove some slides at certain intervals, and keep heating the rest (replacing the removed slides with blanks, so as to keep the same amount of material inside the machine). My microwave HIER protocols vary in length from 5' up to 20' (in 5' increments, replacing buffer volume if needed), so being able to remove at the right times is essential. I often have 5-6 different times and buffers going, and could not spend the extra time trying to do HIER separately on each one. The downside to using a microwave is the verifiability of temperature, if you don't have a fancy laboratory microwave. I'm currently looking into the various models and price ranges out there for a new one that will record temps, etc. Steam retrieval in a vegetable steamer is great. However, the downside to steam retrieval is the amount of time needed to heat up the slides/solutions. Sixty minutes is just too long for my turn-around time. Most days, I think 20 minutes is too long! Pressure cooker HIER works really well, too, but you run into a problem if all your tests for that day don't use the same amount of time in HIER. Once you break that seal to remove some of the slides that need a short heating time, your temp/pressure are gone. I work in an animal diagnostic lab, and our usual workload is about 150 slides/day, so we need to get the slides in and out fairly rapidly. Thus, the reason why microwaving works best for me. Others will have different needs and time allowances in their labs. P.S. a) I always start with cold buffer prior to heat retrieval. b) I always cooldown in HIER buffers for 20'. Jan Shivers UMN VDL ----- Original Message ----- From: "Perry, Margaret" To: ; Sent: Wednesday, October 22, 2008 11:28 AM Subject: [Histonet] HIER We have been using the microwave HIER and have had good results, however when our microwave bits the dust I would like to have a pressure cooker method in place. I feel the pressure cooker is more consistent for all the slides. We are a veterinary diagnostic lab and I would like to have some idea of where to begin. I have looked at different protocols and they often indicate HIER in a pressure cooker but do not give the details. I currently use citrate buffer pH 6. I put the slides in refrigerated buffer and microwave on high for 1 min 45 sec. or until the buffer just starts to boil. I then set the microwave on 10% power for 10 minutes. Afterward the slides are allowed to cool in the microwave for 1 hour. We have a biocare Decloaker Chamber and I would appreciate help with the program I should use. Do you start with cold buffer or should I prewarm it? What temperature should I use? How long should I maintain the temperature? How long should it be before I remove the slides? I also am working with a new protocol that calls for heating in a steamer. Should the temperature of the buffer be warm, cold or room temp when I start? Thank you. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Wed Oct 22 13:31:02 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Wed Oct 22 13:31:05 2008 Subject: [Histonet] HIER Message-ID: <614593.32143.qm@web53608.mail.re2.yahoo.com> I'm wondering if anyone out there has tried something called Uni-Trieve from Innovex Biosciences that claims to be a universal low temperature retrieval solution ie: 75 degrees for 15 minutes. I wouldn't mind trying it on bone sections to see if they stay on better. ? Larry A. Woody Seattle, Wa. ----- Original Message ---- From: Jan Shivers To: "Perry, Margaret" ; ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Sent: Wednesday, October 22, 2008 11:23:05 AM Subject: Re: [Histonet] HIER In my opinion/experience: The upside of using a microwave for HIER is that you can remove some slides at certain intervals, and keep heating the rest (replacing the removed slides with blanks, so as to keep the same amount of material inside the machine).? My microwave HIER protocols vary in length from 5' up to 20' (in 5' increments, replacing buffer volume if needed), so being able to remove at the right times is essential.? I often have 5-6 different times and buffers going, and could not spend the extra time trying to do HIER separately on each one.? The downside to using a microwave is the verifiability of temperature, if you don't have a fancy laboratory microwave.? I'm currently looking into the various models and price ranges out there for a new one that will record temps, etc. Steam retrieval in a vegetable steamer is great.? However, the downside to steam retrieval is the amount of time needed to heat up the slides/solutions.? Sixty minutes is just too long for my turn-around time. Most days, I think 20 minutes is too long! Pressure cooker HIER works really well, too, but you run into a problem if all your tests for that day don't use the same amount of time in HIER.? Once you break that seal to remove some of the slides that need a short heating time, your temp/pressure are gone. I work in an animal diagnostic lab, and our usual workload is about 150 slides/day, so we need to get the slides in and out fairly rapidly.? Thus, the reason why microwaving works best for me.? Others will have different needs and time allowances in their labs. P.S.? a) I always start with cold buffer prior to heat retrieval.? b)? I always cooldown in HIER buffers for 20'. Jan Shivers UMN VDL ----- Original Message ----- From: "Perry, Margaret" To: ; Sent: Wednesday, October 22, 2008 11:28 AM Subject: [Histonet] HIER We have been using the microwave HIER and have had good results, however when our microwave bits the dust I would like to have a pressure cooker method in place.? I feel the pressure cooker is more consistent for all the slides.? We are a veterinary diagnostic lab and I would like to have some idea of where to begin.? I have looked at different protocols and they often indicate HIER in a pressure cooker but do not give the details.? I currently use citrate buffer pH 6.? I put the slides in refrigerated buffer and microwave on high for 1 min 45 sec. or until the buffer just starts to boil. I then set the microwave on 10% power for 10 minutes.? Afterward the slides are allowed to cool in the microwave for 1 hour.? We have a biocare Decloaker Chamber and I would appreciate help with the program I should use. Do you start with cold buffer or should I prewarm it?? What temperature should I use? How long should I maintain the temperature?? How long should it be before I remove the slides? I also am working with a new protocol that calls for heating in a steamer. Should the temperature of the buffer be warm, cold or room temp when I start? Thank you. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKnutson <@t> primecare.org Wed Oct 22 13:32:22 2008 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Wed Oct 22 13:31:53 2008 Subject: [Histonet] Specimen Handling Procedure for Breast Specimens with Predictive Markers Message-ID: <4F0B7161A6CD524FAD8017D52E155340090678B0@exchangent> With the new CAP regulations concerning Her-2/neu and Predictive Markers on breast specimens, has anyone let the processed specimens sit at room temp for a length of time before embedding? We are not a 24/7 staffed lab and would appreciate any input on how others deal with these regulations. Deanne From b-frederick <@t> northwestern.edu Wed Oct 22 13:46:23 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Oct 22 13:46:35 2008 Subject: [Histonet] Question about Leica Autostainer XL In-Reply-To: <16C83872A53F4346AA9C3A18E3A3AAB903F76E6F@VHAV10MSGA1.v10.med.va.gov> Message-ID: <000101c93476$7e65dd00$d00f7ca5@lurie.northwestern.edu> We have also noticed that we get preciptate and mold in the water drain hose. We bleach it once a month along with the water containers. Our hose is not smooth (corrugated?) Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weber, Susan (VHACLE) Sent: Wednesday, October 22, 2008 11:00 AM To: Clouse, Rosanna; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Question about Leica Autostainer XL Have you removed all the reagent containers? Even the water containers? We have noticed that without regular flushing of machine with a weak bleach solution (or disinfectant) we have mold growth which can be the cause of odor in the equipment. Not the reagent containers, the actual chamber interior. I had not noticed any extreme warmth coming from the arm mechanism,that is something you can ask the Leica rep or service rep. I have always found our rep and the general service of Leica to be very helpful and responsive. They may be able to pinpoint the source of the odor also. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clouse, Rosanna Sent: Wednesday, October 22, 2008 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about Leica Autostainer XL I have a question about a Leica Autostainer XL. We are getting the most peculiar odor from it during staining and none of us can quite describe it. One of our histotechs is very sensitive to it however. We have changed the filters and the odor is still there. Also, the arm mechanism is very warm--almost hot--and we were wondering if this is normal for operation. Thanks so much for any and all comments. Rosanna S. Clouse, SCT(ASCP) Division Manager, Cytology Gettysburg Hospital-Wellspan 147 Gettys Street Gettysburg, PA 17325 Phone: 717-337-4120 (x75134) Fax: 717-337-4236 rclouse@wellspan.org CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Wed Oct 22 15:05:41 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Oct 22 15:05:46 2008 Subject: [Histonet] Specimen Handling Procedure for Breast Specimens withPredictive Markers Message-ID: <57BE698966D5C54EAE8612E8941D7683040AF967@EXCHANGE3.huntingtonhospital.com> We don't work on the weekends, so the pathologist takes the breasts off of the processor on Sunday morning and puts them in a covered container. On Monday morning, we melt them down and embed them. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne Sent: Wednesday, October 22, 2008 11:32 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Specimen Handling Procedure for Breast Specimens withPredictive Markers With the new CAP regulations concerning Her-2/neu and Predictive Markers on breast specimens, has anyone let the processed specimens sit at room temp for a length of time before embedding? We are not a 24/7 staffed lab and would appreciate any input on how others deal with these regulations. Deanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbarone <@t> NEMOURS.ORG Wed Oct 22 15:31:19 2008 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Wed Oct 22 15:31:35 2008 Subject: [Histonet] DiI staining Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE80147181F@wlmmsx01.nemours.org> continued from previous question submitted 10.28...now found dye-I ...actually called Di I staining (DiI)...now looking for a protocol ...with good way to prevent bleeding into the tissue after injection and before cutting of sections...found a protocol from 1998...but, hoping for a better way..something more recent, perhaps.....Not assay! ...for frozen sections mouse. From peddinti_2002us <@t> yahoo.co.in Wed Oct 22 17:56:58 2008 From: peddinti_2002us <@t> yahoo.co.in (kamal prasad) Date: Wed Oct 22 18:03:46 2008 Subject: [Histonet] Pressure cooker antigen retrieval Message-ID: <328585.64877.qm@web94706.mail.in2.yahoo.com> Hi Margaret Perry , You can put your slides in to cold solution and put in to pressure cooker then you can set the temperature?to 95 C for 12 - 15 min is fine or after reaching to 95 C you can keep it for? 6 - 8 min is good. This protocol is good for when you use 10% Neutral buffered formaline as a fixative. Regards, Kamal Share files, take polls, and make new friends - all under one roof. Go to http://in.promos.yahoo.com/groups/ From marktarango <@t> gmail.com Wed Oct 22 23:54:00 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Oct 22 23:54:04 2008 Subject: [Histonet] 88305 pod lab question Message-ID: <5b6eb13e0810222154m2b17a797w98875a5c21683619@mail.gmail.com> Can a pathologist who has a "lab" but the lab is just the pathologist's microscope (because histology is outsourced) charge 88305 and get the money for the technicial component? I thought this was against the rules... Anyone know? Mark From mpatrick00 <@t> hotmail.com Thu Oct 23 03:09:42 2008 From: mpatrick00 <@t> hotmail.com (Michael Patric) Date: Thu Oct 23 03:09:51 2008 Subject: [Histonet] peristaltic pump setup/storage of frozen rat brain Message-ID: Hello Everyone, A colleague recommended this group to me. I hope you will be able to help me with three questions that I have: 1) I am trying to setup a Masterflex L/S peristaltic economy drive pump system to assist in the perfusion of adult rats. We are interested in collecting the brain for later immunohistichemistry/in situ studies. I would like to know what the appropriate type of tubing and tube size should be for this setup? 2) I am embedding these fixed brains in OCT for freezing with N2/isopentane. I have been trying to find molds that are of appropriate size for freezing an entire adult rat brain. The commerical molds (Tissue Tek) I have seen are either too big or too small for freezing an adult rat brain en bloc. Does anyone have a recommedation or do they use something completely different? Also is better cut these brains into smaller sections and freezing them rather freezing the entire brain en block? 3) Finally, can someone recommend their procedure for storing rat brains (probably will be the same for any biological tissue) in a -80 freezer for long-term storage? Appreciate the help, Michael Patrick _________________________________________________________________ From nyilmaz <@t> mersin.edu.tr Thu Oct 23 05:54:25 2008 From: nyilmaz <@t> mersin.edu.tr (Nejat Yilmaz) Date: Thu Oct 23 05:56:13 2008 Subject: [Histonet] need help for cultured cells Message-ID: <001101c934fd$bb8ab610$2101a8c0@nejat1> Dear Netters, How can I set 50.000 cells in 1 ul suspension from a cultured cell pellet? I need your kindly help. Best regards... Dr. Necat Yilmaz From godsgalnow <@t> aol.com Thu Oct 23 07:23:36 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Oct 23 07:23:54 2008 Subject: [Histonet] 88305 pod lab question In-Reply-To: <5b6eb13e0810222154m2b17a797w98875a5c21683619@mail.gmail.com> References: <5b6eb13e0810222154m2b17a797w98875a5c21683619@mail.gmail.com> Message-ID: <8CB03214EA905A7-468-709@WEBMAIL-DZ06.sysops.aol.com> If the histology is outsourced the technical portion cannot be charged.? Although, I know some pod labs, that have found loopholes to this--that is whay I no longer work for one. Roxanne -----Original Message----- From: Mark Tarango To: histonet Sent: Thu, 23 Oct 2008 12:54 am Subject: [Histonet] 88305 pod lab question Can a pathologist who has a "lab" but the lab is just the pathologist's microscope (because histology is outsourced) charge 88305 and get the money for the technicial component? I thought this was against the rules... Anyone know? Mark _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Oct 23 08:15:47 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Oct 23 08:15:54 2008 Subject: [Histonet] RE: [IHCRG] HIER In-Reply-To: Message-ID: I have used many different hier methods over the years and have special considerations because of my altitude. We thought that the pressure cooker would solve all the problems relating to altitude because it is pressurized but it did not. There must be some boiling going on sometime because I often got damaged tissue with the PC. My heat source of choice now includes either a waterbath or steamer, and I use the steamer most often because water boils rapidly above about 92c for me, so I have to keep the waterbath below that. My husband drilled a hole in the lid of the steamer where I insert a thermometer. I use a digital cooking therm., it has a feature that you can set to alarm when it reaches a programmed temp, I put tubs or coplin jars with the buffers in the steamer to warm up while I deparaffinize the slides. I put the temp probe in the solution in the steamer and set it to alarm at 90c. after I put the slides in the buffer in the steamer, I set the alarm again to go off when it again reaches 90c, (it can take up to 10-15 min. to get back to temp after adding the slides) after it reaches 90c with the slides in there I start timing, I steam my slides for 15-20 min after they reach 90+c., they will go up only to about 95c in my lab because of the altitude I assume. When they are done I take them out of the steamer and let them cool on the counter for 20 min or so, then rinse in water and then place in buffer before doing IHC. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org _____ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Perry, Margaret Sent: Wednesday, October 22, 2008 10:28 AM To: ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: [IHCRG] HIER We have been using the microwave HIER and have had good results, however when our microwave bits the dust I would like to have a pressure cooker method in place. I feel the pressure cooker is more consistent for all the slides. We are a veterinary diagnostic lab and I would like to have some idea of where to begin. I have looked at different protocols and they often indicate HIER in a pressure cooker but do not give the details. I currently use citrate buffer pH 6. I put the slides in refrigerated buffer and microwave on high for 1 min 45 sec. or until the buffer just starts to boil. I then set the microwave on 10% power for 10 minutes. Afterward the slides are allowed to cool in the microwave for 1 hour. We have a biocare Decloaker Chamber and I would appreciate help with the program I should use. Do you start with cold buffer or should I prewarm it? What temperature should I use? How long should I maintain the temperature? How long should it be before I remove the slides? I also am working with a new protocol that calls for heating in a steamer. Should the temperature of the buffer be warm, cold or room temp when I start? Thank you. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- From fudo <@t> ufl.edu Thu Oct 23 08:15:53 2008 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Thu Oct 23 08:16:05 2008 Subject: [Histonet] TUNEL on mouse bone marrow Message-ID: <907980917.27921224767753540.JavaMail.osg@osgjas01.cns.ufl.edu> Hi, all We met a problem with TUNEL staining on mouse BM(10% NBF fixed paraffin). The protocol we used works very well with other tissues, however, it gave us high background on bone marrow. Can anyone give us some suggestions on TUNEL staining using bone marrow? Many thanks, Ann Dongtao Fu MD, Ph.D Lab Manager Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 From jamie.erickson <@t> abbott.com Thu Oct 23 08:20:34 2008 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Thu Oct 23 08:20:49 2008 Subject: [Histonet] Manual processing of radioactive mouse brains Message-ID: Hello histoneters, I have a question for anyone out in histoland that manually processes tissue. I have a study that will be using radioactive I125 mouse brain tissue and I don't want to process these in our Lecia TP 1050 auto processor for risk of contaminating the unit. The other researcher in the lab would not like me much if contaminated our only processor...... So I need a good quick manual process protocol for brain, spinal cord, liver tissues. I have been using paraformaldehyde fixed frozen brains and sectioning on the cryostat but I want the best morphology and best sections I can get so I'm thinking of trying paraffin. Should I use a desiccation/vacuum chamber to increase my infiltration? Since I'm using I125 with a 1/2 life of 59 days I want to get these processed quickly so I can do microautoradiography... Any help is greatly appreciated... Thanks Jamie _______________________________ Jamie Erickson Sr. Research Associate II Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From tkngflght <@t> yahoo.com Thu Oct 23 08:34:11 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Oct 23 08:34:22 2008 Subject: Technical vs. Professional Re: [Histonet] 88305 pod lab question In-Reply-To: <8CB03214EA905A7-468-709@WEBMAIL-DZ06.sysops.aol.com> Message-ID: <139283.83426.qm@web50909.mail.re2.yahoo.com> Hi Mark-- ? The technical and professional on this code?are generally bundled.??For most insurances as well as M/M?it?can't be?split it to be reimbursed (and it HAS to be billed by the party performing the professional is my understanding after just working on this issue?a few months ago), Thus the code can be charged by the path if he or she is subcontracting the technical.? The technical portion is something ridiculously small,?like $12/code.? Chances are the Pathologist is paying more than this for the tech service. ? This is done with frozens all the time where the pathologist is working for?one company? (Path group is independent non-employee entity?of a facility) and the tech who supports them work for the hospital and with surgical stuff where the pathology group is a contractor for the hospital that employs the tech... ? Same animal, different zoo. ? Cheryl ? Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time. 281.913.7285 800.756.3308 ? ? From mthomas <@t> littonlab.com Thu Oct 23 08:57:44 2008 From: mthomas <@t> littonlab.com (Marla Thomas) Date: Thu Oct 23 08:58:00 2008 Subject: Technical vs. Professional Re: [Histonet] 88305 pod lab question In-Reply-To: <139283.83426.qm@web50909.mail.re2.yahoo.com> Message-ID: <200810231357.m9NDvigM011549@smtp01.atlngahp.sys.nuvox.net> If I am not mistaken, for MD/MC it is the unit performing the technical who can bill for the global component. If you only do the professional component you cannot bill for the technical. That is why with most POD Labs they must provide the technical themselves in order to bill globally. Many of the commercial insurance companies do allow anyone to bill globally whether they do the technical or not. In those instances they can "purchase" the "technical" work to be done somewhere else. If I am remembering right... Marla Thomas, HT(ASCP) Litton Pathology Associates, PC 700 NW Hunter Dr. Blue Springs, MO 64015 Phone: 816-229-6449 Fax:816-874-4400 CONFIDENTIALITY NOTICE This message and any included attachments are from Litton Pathology Associates, P.C. and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 816-229-6449 and ask for the HIPAA/Compliance Coordinator. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Thursday, October 23, 2008 8:34 AM To: marktarango@gmail.com; Histonet@lists.utsouthwestern.edu; godsgalnow@aol.com Subject: Technical vs. Professional Re: [Histonet] 88305 pod lab question Hi Mark-- ? The technical and professional on this code?are generally bundled.??For most insurances as well as M/M?it?can't be?split it to be reimbursed (and it HAS to be billed by the party performing the professional is my understanding after just working on this issue?a few months ago), Thus the code can be charged by the path if he or she is subcontracting the technical.? The technical portion is something ridiculously small,?like $12/code.? Chances are the Pathologist is paying more than this for the tech service. ? This is done with frozens all the time where the pathologist is working for?one company? (Path group is independent non-employee entity?of a facility) and the tech who supports them work for the hospital and with surgical stuff where the pathology group is a contractor for the hospital that employs the tech... ? Same animal, different zoo. ? Cheryl ? Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time. 281.913.7285 800.756.3308 ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Thu Oct 23 08:54:34 2008 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Oct 23 09:08:05 2008 Subject: [Histonet] TUNEL on mouse bone marrow Message-ID: <1581245502-1224770872-cardhu_decombobulator_blackberry.rim.net-1744216408-@bxe270.bisx.prod.on.blackberry> I had the same experience and thought it was most likely due to the recombination that occurs in the BM during lymphocyte maturation - so the reagents are giving false positives. So instead I use cleaved caspase 3 IHC. ------Original Message------ From: FU,DONGTAO Sender: histonet-bounces@lists.utsouthwestern.edu To: Histonet@lists.utsouthwestern.edu Sent: Oct 23, 2008 9:15 AM Subject: [Histonet] TUNEL on mouse bone marrow Hi, all We met a problem with TUNEL staining on mouse BM(10% NBF fixed paraffin). The protocol we used works very well with other tissues, however, it gave us high background on bone marrow. Can anyone give us some suggestions on TUNEL staining using bone marrow? Many thanks, Ann Dongtao Fu MD, Ph.D Lab Manager Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Thu Oct 23 08:57:44 2008 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Oct 23 09:08:09 2008 Subject: [Histonet] RE: [IHCRG] HIER In-Reply-To: <0K9700KKS10FFQD0@mail-gw1.med.cornell.edu> References: <0K9700KKS10FFQD0@mail-gw1.med.cornell.edu> Message-ID: <892141032-1224770875-cardhu_decombobulator_blackberry.rim.net-1251705890-@bxe270.bisx.prod.on.blackberry> I too am all about the steamer. We use a veggie steamer (only costs about 10-15 dollars) and heat the solution to 95 deg C for a 20 min retrieval. Works great. -----Original Message----- From: Patsy Ruegg Date: Thu, 23 Oct 2008 07:15:47 To: ; ; Subject: [Histonet] RE: [IHCRG] HIER I have used many different hier methods over the years and have special considerations because of my altitude. We thought that the pressure cooker would solve all the problems relating to altitude because it is pressurized but it did not. There must be some boiling going on sometime because I often got damaged tissue with the PC. My heat source of choice now includes either a waterbath or steamer, and I use the steamer most often because water boils rapidly above about 92c for me, so I have to keep the waterbath below that. My husband drilled a hole in the lid of the steamer where I insert a thermometer. I use a digital cooking therm., it has a feature that you can set to alarm when it reaches a programmed temp, I put tubs or coplin jars with the buffers in the steamer to warm up while I deparaffinize the slides. I put the temp probe in the solution in the steamer and set it to alarm at 90c. after I put the slides in the buffer in the steamer, I set the alarm again to go off when it again reaches 90c, (it can take up to 10-15 min. to get back to temp after adding the slides) after it reaches 90c with the slides in there I start timing, I steam my slides for 15-20 min after they reach 90+c., they will go up only to about 95c in my lab because of the altitude I assume. When they are done I take them out of the steamer and let them cool on the counter for 20 min or so, then rinse in water and then place in buffer before doing IHC. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org _____ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Perry, Margaret Sent: Wednesday, October 22, 2008 10:28 AM To: ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: [IHCRG] HIER We have been using the microwave HIER and have had good results, however when our microwave bits the dust I would like to have a pressure cooker method in place. I feel the pressure cooker is more consistent for all the slides. We are a veterinary diagnostic lab and I would like to have some idea of where to begin. I have looked at different protocols and they often indicate HIER in a pressure cooker but do not give the details. I currently use citrate buffer pH 6. I put the slides in refrigerated buffer and microwave on high for 1 min 45 sec. or until the buffer just starts to boil. I then set the microwave on 10% power for 10 minutes. Afterward the slides are allowed to cool in the microwave for 1 hour. We have a biocare Decloaker Chamber and I would appreciate help with the program I should use. Do you start with cold buffer or should I prewarm it? What temperature should I use? How long should I maintain the temperature? How long should it be before I remove the slides? I also am working with a new protocol that calls for heating in a steamer. Should the temperature of the buffer be warm, cold or room temp when I start? Thank you. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Thu Oct 23 09:17:14 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Oct 23 09:17:24 2008 Subject: Technical vs. Professional Re: [Histonet] 88305 pod lab question In-Reply-To: <200810231357.m9NDvigM011549@smtp01.atlngahp.sys.nuvox.net> Message-ID: <191987.76888.qm@web50912.mail.re2.yahoo.com> Can anyone clear this up with certainty?? We aren't billing MC/MD so?Marla?may be totally?correct on this.? I read the full code book as published last year?and didn't come away with quite this same distinction and I would really like to know! ? Cheryl ? Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time. 281.913.7285 800.756.3308 --- On Thu, 10/23/08, Marla Thomas wrote: From: Marla Thomas Subject: RE: Technical vs. Professional Re: [Histonet] 88305 pod lab question To: "'Cheryl'" , marktarango@gmail.com, Histonet@lists.utsouthwestern.edu, godsgalnow@aol.com Date: Thursday, October 23, 2008, 6:57 AM If I am not mistaken, for MD/MC it is the unit performing the technical who can bill for the global component. If you only do the professional component you cannot bill for the technical. That is why with most POD Labs they must provide the technical themselves in order to bill globally. Many of the commercial insurance companies do allow anyone to bill globally whether they do the technical or not. In those instances they can "purchase" the "technical" work to be done somewhere else. If I am remembering right... Marla Thomas, HT(ASCP) Litton Pathology Associates, PC 700 NW Hunter Dr. Blue Springs, MO 64015 Phone: 816-229-6449 Fax:816-874-4400 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Thursday, October 23, 2008 8:34 AM To: marktarango@gmail.com; Histonet@lists.utsouthwestern.edu; godsgalnow@aol.com Subject: Technical vs. Professional Re: [Histonet] 88305 pod lab question Hi Mark-- ? The technical and professional on this code?are generally bundled.??For most insurances as well as M/M?it?can't be?split it to be reimbursed (and it HAS to be billed by the party performing the professional is my understanding after just working on this issue?a few months ago), Thus the code can be charged by the path if he or she is subcontracting the technical.? The technical portion is something ridiculously small,?like $12/code.? Chances are the Pathologist is paying more than this for the tech service. ? This is done with frozens all the time where the pathologist is working for?one company? (Path group is independent non-employee entity?of a facility) and the tech who supports them work for the hospital and with surgical stuff where the pathology group is a contractor for the hospital that employs the tech... ? Same animal, different zoo. ? Cheryl ? Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time. 281.913.7285 800.756.3308 ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Hooten <@t> medinst.com Thu Oct 23 09:55:26 2008 From: Hooten <@t> medinst.com (Scott Hooten) Date: Thu Oct 23 09:55:40 2008 Subject: [Histonet] Celestine Blue Message-ID: <4900581E.8FD5.00E5.0@medinst.com> I was wondering if anyone knew of any place to buy pre-made celestine blue. Right now we have to buy the separate chemicals and mix up our own, so if anyone knows of a place that sells pre-made celestine blue I would appreciate it. Thanks. Scott R. Hooten Histology Technician MED Institute 1 Geddes Way West Lafayette, IN 47906 765-464-0817 ext. 1115 From marktarango <@t> gmail.com Thu Oct 23 10:20:12 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Oct 23 10:20:20 2008 Subject: [Histonet] 88305 pod lab question In-Reply-To: <8CB03214EA905A7-468-709@WEBMAIL-DZ06.sysops.aol.com> References: <5b6eb13e0810222154m2b17a797w98875a5c21683619@mail.gmail.com> <8CB03214EA905A7-468-709@WEBMAIL-DZ06.sysops.aol.com> Message-ID: <5b6eb13e0810230820v6a83006bx267105dc63f3cda@mail.gmail.com> Well just like Roxanne I quit my job over this. I brought up the way he charges in the job interview and he claimed that he had letters from lawyers that said it was okay and that he DIDN'T actually bill medicare for 88305-TC, but only insurance companies. When I started working there in billing dept, I found myself charging 88305-TC to medicare. He obviously lied. They only pay to the histo lab a small portion of the reimbursement. They want me to go back and now the docs say I can setup a lab to make the operation legit. I'm worried if I should even cash the paycheck... back on the job hunt... Thanks for the responses, Mark On 10/23/08, godsgalnow@aol.com wrote: > If the histology is outsourced the technical portion cannot be charged. > Although, I know some pod labs, that have found loopholes to this--that is > whay I no longer work for one. > > Roxanne > > > -----Original Message----- > From: Mark Tarango > To: histonet > Sent: Thu, 23 Oct 2008 12:54 am > Subject: [Histonet] 88305 pod lab question > > Can a pathologist who has a "lab" but the lab is just the pathologist's > microscope (because histology is outsourced) charge 88305 and get the money > for the technicial component? I thought this was against the rules... > > Anyone know? > > Mark > _______________________________________________ > Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > McCain or Obama? Stay updated on coverage of the Presidential race while > you browse - Download Now! > From marktarango <@t> gmail.com Thu Oct 23 10:33:31 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Oct 23 10:33:40 2008 Subject: Technical vs. Professional Re: [Histonet] 88305 pod lab question In-Reply-To: <191987.76888.qm@web50912.mail.re2.yahoo.com> References: <200810231357.m9NDvigM011549@smtp01.atlngahp.sys.nuvox.net> <191987.76888.qm@web50912.mail.re2.yahoo.com> Message-ID: <5b6eb13e0810230833u39bd38d1n59d1a940b30f518@mail.gmail.com> Hi Cheryl, I looked up 88305-TC and it looks like they pay around $60 for this code. 88305-26(professional component) only pays around $40 depending on locality. They pay more to make the slide than read it. Mark On 10/23/08, Cheryl wrote: > Can anyone clear this up with certainty? We aren't billing MC/MD so Marla > may be totally correct on this. I read the full code book as published last > year and didn't come away with quite this same distinction and I would > really like to know! > > Cheryl > > Full Staff Inc. > Staffing Healthcare Professionals - One GREAT fit at a time. > 281.913.7285 > 800.756.3308 > > > --- On Thu, 10/23/08, Marla Thomas wrote: > > From: Marla Thomas > Subject: RE: Technical vs. Professional Re: [Histonet] 88305 pod lab > question > To: "'Cheryl'" , marktarango@gmail.com, > Histonet@lists.utsouthwestern.edu, godsgalnow@aol.com > Date: Thursday, October 23, 2008, 6:57 AM > > If I am not mistaken, for MD/MC it is the unit performing the technical who > can bill for the global component. If you only do the professional > component you cannot bill for the technical. That is why with most POD > Labs > they must provide the technical themselves in order to bill globally. > > Many of the commercial insurance companies do allow anyone to bill globally > whether they do the technical or not. In those instances they can > "purchase" the "technical" work to be done somewhere else. > > If I am remembering right... > > Marla Thomas, HT(ASCP) > > Litton Pathology Associates, PC > > 700 NW Hunter Dr. > > Blue Springs, MO 64015 > > Phone: 816-229-6449 Fax:816-874-4400 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl > Sent: Thursday, October 23, 2008 8:34 AM > To: marktarango@gmail.com; Histonet@lists.utsouthwestern.edu; > godsgalnow@aol.com > Subject: Technical vs. Professional Re: [Histonet] 88305 pod lab question > > Hi Mark-- > > The technical and professional on this code are generally bundled. For > most > insurances as well as M/M it can't be split it to be reimbursed (and it > HAS > to be billed by the party performing the professional is my understanding > after just working on this issue a few months ago), Thus the code can be > charged by the path if he or she is subcontracting the technical. The > technical portion is something ridiculously small, like $12/code. Chances > are the Pathologist is paying more than this for the tech service. > > This is done with frozens all the time where the pathologist is working > for one company (Path group is independent non-employee entity of a > facility) and the tech who supports them work for the hospital and with > surgical stuff where the pathology group is a contractor for the hospital > that employs the tech... > > Same animal, different zoo. > > Cheryl > > Full Staff Inc. > Staffing Healthcare Professionals - One GREAT fit at a time. > 281.913.7285 > 800.756.3308 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWeems <@t> sjha.org Thu Oct 23 10:37:30 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Oct 23 10:38:59 2008 Subject: Technical vs. Professional Re: [Histonet] 88305 pod lab question In-Reply-To: <5b6eb13e0810230833u39bd38d1n59d1a940b30f518@mail.gmail.com> References: <200810231357.m9NDvigM011549@smtp01.atlngahp.sys.nuvox.net><191987.76888.qm@web50912.mail.re2.yahoo.com> <5b6eb13e0810230833u39bd38d1n59d1a940b30f518@mail.gmail.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA50E3C39@ITSSSXM01V6.one.ads.che.org> Which is why everyone is trying to scramble the gray areas to make it work for them, including urologists who are taking money away from the pathologists. We all need to work to correct this process before it gets totally out of hand. For some reason beyond me, our politicians cannot understand this. We need to bombard them to make changes. The ruling regarding pod labs is on hold. My two cents...j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, October 23, 2008 11:34 AM To: Cheryl Cc: histonet@lists.utsouthwestern.edu Subject: Re: Technical vs. Professional Re: [Histonet] 88305 pod lab question Hi Cheryl, I looked up 88305-TC and it looks like they pay around $60 for this code. 88305-26(professional component) only pays around $40 depending on locality. They pay more to make the slide than read it. Mark On 10/23/08, Cheryl wrote: > Can anyone clear this up with certainty? We aren't billing MC/MD so > Marla may be totally correct on this. I read the full code book as > published last year and didn't come away with quite this same > distinction and I would really like to know! > > Cheryl > > Full Staff Inc. > Staffing Healthcare Professionals - One GREAT fit at a time. > 281.913.7285 > 800.756.3308 > > > --- On Thu, 10/23/08, Marla Thomas wrote: > > From: Marla Thomas > Subject: RE: Technical vs. Professional Re: [Histonet] 88305 pod lab > question > To: "'Cheryl'" , marktarango@gmail.com, > Histonet@lists.utsouthwestern.edu, godsgalnow@aol.com > Date: Thursday, October 23, 2008, 6:57 AM > > If I am not mistaken, for MD/MC it is the unit performing the > technical who can bill for the global component. If you only do the > professional component you cannot bill for the technical. That is why > with most POD Labs they must provide the technical themselves in order > to bill globally. > > Many of the commercial insurance companies do allow anyone to bill > globally whether they do the technical or not. In those instances > they can "purchase" the "technical" work to be done somewhere else. > > If I am remembering right... > > Marla Thomas, HT(ASCP) > > Litton Pathology Associates, PC > > 700 NW Hunter Dr. > > Blue Springs, MO 64015 > > Phone: 816-229-6449 Fax:816-874-4400 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl > Sent: Thursday, October 23, 2008 8:34 AM > To: marktarango@gmail.com; Histonet@lists.utsouthwestern.edu; > godsgalnow@aol.com > Subject: Technical vs. Professional Re: [Histonet] 88305 pod lab > question > > Hi Mark-- > > The technical and professional on this code are generally bundled. > For most insurances as well as M/M it can't be split it to be > reimbursed (and it HAS to be billed by the party performing the > professional is my understanding after just working on this issue a > few months ago), Thus the code can be charged by the path if he or she > is subcontracting the technical. The technical portion is something > ridiculously small, like $12/code. Chances are the Pathologist is > paying more than this for the tech service. > > This is done with frozens all the time where the pathologist is > working for one company (Path group is independent non-employee > entity of a > facility) and the tech who supports them work for the hospital and > with surgical stuff where the pathology group is a contractor for the > hospital that employs the tech... > > Same animal, different zoo. > > Cheryl > > Full Staff Inc. > Staffing Healthcare Professionals - One GREAT fit at a time. > 281.913.7285 > 800.756.3308 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From lblazek <@t> digestivespecialists.com Thu Oct 23 10:48:10 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Oct 23 10:47:14 2008 Subject: Technical vs. Professional Re: [Histonet] 88305 pod lab question In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA50E3C39@ITSSSXM01V6.one.ads.che.org> References: <200810231357.m9NDvigM011549@smtp01.atlngahp.sys.nuvox.net><191987.76888.qm@web50912.mail.re2.yahoo.com> <5b6eb13e0810230833u39bd38d1n59d1a940b30f518@mail.gmail.com> <5D64396A0D4A5346BEBC759022AAEAA50E3C39@ITSSSXM01V6.one.ads.che.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E3907B6FE1455@IBMB7Exchange.digestivespecialists.com> What is the definition of a Pod Lab? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, October 23, 2008 11:38 AM To: Mark Tarango; Cheryl Cc: histonet@lists.utsouthwestern.edu Subject: RE: Technical vs. Professional Re: [Histonet] 88305 pod lab question Which is why everyone is trying to scramble the gray areas to make it work for them, including urologists who are taking money away from the pathologists. We all need to work to correct this process before it gets totally out of hand. For some reason beyond me, our politicians cannot understand this. We need to bombard them to make changes. The ruling regarding pod labs is on hold. My two cents...j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, October 23, 2008 11:34 AM To: Cheryl Cc: histonet@lists.utsouthwestern.edu Subject: Re: Technical vs. Professional Re: [Histonet] 88305 pod lab question Hi Cheryl, I looked up 88305-TC and it looks like they pay around $60 for this code. 88305-26(professional component) only pays around $40 depending on locality. They pay more to make the slide than read it. Mark On 10/23/08, Cheryl wrote: > Can anyone clear this up with certainty? We aren't billing MC/MD so > Marla may be totally correct on this. I read the full code book as > published last year and didn't come away with quite this same > distinction and I would really like to know! > > Cheryl > > Full Staff Inc. > Staffing Healthcare Professionals - One GREAT fit at a time. > 281.913.7285 > 800.756.3308 > > > --- On Thu, 10/23/08, Marla Thomas wrote: > > From: Marla Thomas > Subject: RE: Technical vs. Professional Re: [Histonet] 88305 pod lab > question > To: "'Cheryl'" , marktarango@gmail.com, > Histonet@lists.utsouthwestern.edu, godsgalnow@aol.com > Date: Thursday, October 23, 2008, 6:57 AM > > If I am not mistaken, for MD/MC it is the unit performing the > technical who can bill for the global component. If you only do the > professional component you cannot bill for the technical. That is why > with most POD Labs they must provide the technical themselves in order > to bill globally. > > Many of the commercial insurance companies do allow anyone to bill > globally whether they do the technical or not. In those instances > they can "purchase" the "technical" work to be done somewhere else. > > If I am remembering right... > > Marla Thomas, HT(ASCP) > > Litton Pathology Associates, PC > > 700 NW Hunter Dr. > > Blue Springs, MO 64015 > > Phone: 816-229-6449 Fax:816-874-4400 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl > Sent: Thursday, October 23, 2008 8:34 AM > To: marktarango@gmail.com; Histonet@lists.utsouthwestern.edu; > godsgalnow@aol.com > Subject: Technical vs. Professional Re: [Histonet] 88305 pod lab > question > > Hi Mark-- > > The technical and professional on this code are generally bundled. > For most insurances as well as M/M it can't be split it to be > reimbursed (and it HAS to be billed by the party performing the > professional is my understanding after just working on this issue a > few months ago), Thus the code can be charged by the path if he or she > is subcontracting the technical. The technical portion is something > ridiculously small, like $12/code. Chances are the Pathologist is > paying more than this for the tech service. > > This is done with frozens all the time where the pathologist is > working for one company (Path group is independent non-employee > entity of a > facility) and the tech who supports them work for the hospital and > with surgical stuff where the pathology group is a contractor for the > hospital that employs the tech... > > Same animal, different zoo. > > Cheryl > > Full Staff Inc. > Staffing Healthcare Professionals - One GREAT fit at a time. > 281.913.7285 > 800.756.3308 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Oct 23 10:52:19 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Oct 23 10:53:34 2008 Subject: Technical vs. Professional Re: [Histonet] 88305 pod lab question In-Reply-To: <5A2BD13465E061429D6455C8D6B40E3907B6FE1455@IBMB7Exchange.digestivespecialists.com> References: <200810231357.m9NDvigM011549@smtp01.atlngahp.sys.nuvox.net><191987.76888.qm@web50912.mail.re2.yahoo.com><5b6eb13e0810230833u39bd38d1n59d1a940b30f518@mail.gmail.com><5D64396A0D4A5346BEBC759022AAEAA50E3C39@ITSSSXM01V6.one.ads.che.org> <5A2BD13465E061429D6455C8D6B40E3907B6FE1455@IBMB7Exchange.digestivespecialists.com> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA50E3C3F@ITSSSXM01V6.one.ads.che.org> http://www.google.com/search?hl=en&q=pod+labs&aq=f&oq= Check out the info at this google search. It will eventually be stopped, I'm sure, but not before a lot of money is wasted setting up histo labs where they don't need to be and pathologists losing technical fees with global billing, grrrrhhh.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, October 23, 2008 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: RE: Technical vs. Professional Re: [Histonet] 88305 pod lab question What is the definition of a Pod Lab? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, October 23, 2008 11:38 AM To: Mark Tarango; Cheryl Cc: histonet@lists.utsouthwestern.edu Subject: RE: Technical vs. Professional Re: [Histonet] 88305 pod lab question Which is why everyone is trying to scramble the gray areas to make it work for them, including urologists who are taking money away from the pathologists. We all need to work to correct this process before it gets totally out of hand. For some reason beyond me, our politicians cannot understand this. We need to bombard them to make changes. The ruling regarding pod labs is on hold. My two cents...j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, October 23, 2008 11:34 AM To: Cheryl Cc: histonet@lists.utsouthwestern.edu Subject: Re: Technical vs. Professional Re: [Histonet] 88305 pod lab question Hi Cheryl, I looked up 88305-TC and it looks like they pay around $60 for this code. 88305-26(professional component) only pays around $40 depending on locality. They pay more to make the slide than read it. Mark On 10/23/08, Cheryl wrote: > Can anyone clear this up with certainty? We aren't billing MC/MD so > Marla may be totally correct on this. I read the full code book as > published last year and didn't come away with quite this same > distinction and I would really like to know! > > Cheryl > > Full Staff Inc. > Staffing Healthcare Professionals - One GREAT fit at a time. > 281.913.7285 > 800.756.3308 > > > --- On Thu, 10/23/08, Marla Thomas wrote: > > From: Marla Thomas > Subject: RE: Technical vs. Professional Re: [Histonet] 88305 pod lab > question > To: "'Cheryl'" , marktarango@gmail.com, > Histonet@lists.utsouthwestern.edu, godsgalnow@aol.com > Date: Thursday, October 23, 2008, 6:57 AM > > If I am not mistaken, for MD/MC it is the unit performing the > technical who can bill for the global component. If you only do the > professional component you cannot bill for the technical. That is why > with most POD Labs they must provide the technical themselves in order > to bill globally. > > Many of the commercial insurance companies do allow anyone to bill > globally whether they do the technical or not. In those instances > they can "purchase" the "technical" work to be done somewhere else. > > If I am remembering right... > > Marla Thomas, HT(ASCP) > > Litton Pathology Associates, PC > > 700 NW Hunter Dr. > > Blue Springs, MO 64015 > > Phone: 816-229-6449 Fax:816-874-4400 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl > Sent: Thursday, October 23, 2008 8:34 AM > To: marktarango@gmail.com; Histonet@lists.utsouthwestern.edu; > godsgalnow@aol.com > Subject: Technical vs. Professional Re: [Histonet] 88305 pod lab > question > > Hi Mark-- > > The technical and professional on this code are generally bundled. > For most insurances as well as M/M it can't be split it to be > reimbursed (and it HAS to be billed by the party performing the > professional is my understanding after just working on this issue a > few months ago), Thus the code can be charged by the path if he or she > is subcontracting the technical. The technical portion is something > ridiculously small, like $12/code. Chances are the Pathologist is > paying more than this for the tech service. > > This is done with frozens all the time where the pathologist is > working for one company (Path group is independent non-employee > entity of a > facility) and the tech who supports them work for the hospital and > with surgical stuff where the pathology group is a contractor for the > hospital that employs the tech... > > Same animal, different zoo. > > Cheryl > > Full Staff Inc. > Staffing Healthcare Professionals - One GREAT fit at a time. > 281.913.7285 > 800.756.3308 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From dr_sadushe <@t> yahoo.com Thu Oct 23 12:36:13 2008 From: dr_sadushe <@t> yahoo.com (Sadushe Loxha) Date: Thu Oct 23 12:36:17 2008 Subject: [Histonet] (no subject) Message-ID: <463799.79250.qm@web36108.mail.mud.yahoo.com> From pkromund <@t> gundluth.org Thu Oct 23 12:38:53 2008 From: pkromund <@t> gundluth.org (pkromund@gundluth.org) Date: Thu Oct 23 12:39:01 2008 Subject: [Histonet] Ab validation In-Reply-To: Message-ID: Would you agree that if you change the pretreatment vessel you would have to revalidate all your antibodies? Or if you change your pretreatment solution to an all in one solution that deparaffinizes & does HIER that you would also have to revalidate all your antibodies. Pam Patti Loykasek To Sent by: histonet histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] Ab validation 02/01/2008 01:53 PM ------ Forwarded Message From: Patti Loykasek Date: Fri, 01 Feb 2008 11:18:26 -0800 To: Subject: Re: [Histonet] Re: Histonet Digest, Vol 51, Issue 2 I'll try to formulate a brief answer to the question of validating antibodies. If you are doing IHC both CLIA and CAP have regulations that involve validation of antibodies. These regulations cover Establishment and Verification of Method Performance Specifications. The validation process is designed to confirm the ability of the antibody to recognize the target antigen in normal and diseased tissues where it is reasonably expected to localize. And that there is not any obvious unexpected expression; in other words, to determine its specificity and sensitivity. To validate antibodies for IHC it's a multiple step process. First you would need to determine the working titer, pretreatment, detection & chromogen that you are going to use. Use your standard SOPS. Use a control that contains the antigen in question plus some negative elements. You don't want a control that is a high expressor or too low of an expresser at this point. Once you?ve determined your working parameters, you need to test the antibody on normal tissue & diseased tissue that does & does not contain the antigen. For example, if you are validating an antibody that is positive in Lung carcinomas you would want to use lung carcinomas (should be positive), & a variety of carcinomas, lymphomas, etc... That should be negative. You may find that this antibody is positive in GI carcinoma, too & you would want to document that & know what % of GI carcinomas are positive, Also, run some normal tissues & assess their positive & negative rate. W compare our findings with the published literature. You need to document all this work ( if you don?t document it, it didn?t happen). Excel or some other software is good for documenting your findings. We have a template that we use for all work ups (& a validation SOP). Hope this helps. Good luck. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > > does anyone validate antibody and how do you = do it? > _________________________________________________________________ > > > > > > > > Con= fidentiality Notice: This email message, including any > attachments, is for = the sole use of the intended recipient(s) and > may contain confidential and = privileged information. Any > unauthorized review, use, disclosure or distr= ibution is > prohibited. If you are not the intended recipient, please cont= act > the sender by reply email and destroy all copies of the original > messag= e. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------ End of Forwarded Message This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu Oct 23 12:51:19 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu Oct 23 12:51:25 2008 Subject: [Histonet] Ab validation In-Reply-To: Message-ID: Yes, we would reevaluate and get the new procedures signed-off by our director. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pkromund@gundluth.org Sent: Thursday, October 23, 2008 12:39 PM To: Patti Loykasek Cc: histonet-bounces@lists.utsouthwestern.edu; histonet Subject: Re: [Histonet] Ab validation Would you agree that if you change the pretreatment vessel you would have to revalidate all your antibodies? Or if you change your pretreatment solution to an all in one solution that deparaffinizes & does HIER that you would also have to revalidate all your antibodies. Pam Patti Loykasek To Sent by: histonet histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] Ab validation 02/01/2008 01:53 PM ------ Forwarded Message From: Patti Loykasek Date: Fri, 01 Feb 2008 11:18:26 -0800 To: Subject: Re: [Histonet] Re: Histonet Digest, Vol 51, Issue 2 I'll try to formulate a brief answer to the question of validating antibodies. If you are doing IHC both CLIA and CAP have regulations that involve validation of antibodies. These regulations cover Establishment and Verification of Method Performance Specifications. The validation process is designed to confirm the ability of the antibody to recognize the target antigen in normal and diseased tissues where it is reasonably expected to localize. And that there is not any obvious unexpected expression; in other words, to determine its specificity and sensitivity. To validate antibodies for IHC it's a multiple step process. First you would need to determine the working titer, pretreatment, detection & chromogen that you are going to use. Use your standard SOPS. Use a control that contains the antigen in question plus some negative elements. You don't want a control that is a high expressor or too low of an expresser at this point. Once you?ve determined your working parameters, you need to test the antibody on normal tissue & diseased tissue that does & does not contain the antigen. For example, if you are validating an antibody that is positive in Lung carcinomas you would want to use lung carcinomas (should be positive), & a variety of carcinomas, lymphomas, etc... That should be negative. You may find that this antibody is positive in GI carcinoma, too & you would want to document that & know what % of GI carcinomas are positive, Also, run some normal tissues & assess their positive & negative rate. W compare our findings with the published literature. You need to document all this work ( if you don?t document it, it didn?t happen). Excel or some other software is good for documenting your findings. We have a template that we use for all work ups (& a validation SOP). Hope this helps. Good luck. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > > does anyone validate antibody and how do you = do it? > _________________________________________________________________ > > > > > > > > Con= fidentiality Notice: This email message, including any > attachments, is for = the sole use of the intended recipient(s) and > may contain confidential and = privileged information. Any > unauthorized review, use, disclosure or distr= ibution is > prohibited. If you are not the intended recipient, please cont= act > the sender by reply email and destroy all copies of the original > messag= e. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------ End of Forwarded Message This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail <@t> bellsouth.net Thu Oct 23 13:13:51 2008 From: renafail <@t> bellsouth.net (renafail@bellsouth.net) Date: Thu Oct 23 13:14:02 2008 Subject: [Histonet] Ab validation In-Reply-To: References: Message-ID: <102320081813.19573.4900BEDF0004B7FF00004C7522193100029B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net> Absolutely Rena -------------- Original message from pkromund@gundluth.org: -------------- > Would you agree that if you change the pretreatment vessel you would have > to revalidate all your antibodies? Or if you change your pretreatment > solution to an all in one solution that deparaffinizes & does HIER that you > would also have to revalidate all your antibodies. > Pam > > > > Patti Loykasek > > ath.com> To > Sent by: histonet > histonet-bounces@ > lists.utsouthwest cc > ern.edu > Subject > [Histonet] Ab validation > 02/01/2008 01:53 > PM > > > > > > > > > > ------ Forwarded Message > From: Patti Loykasek > Date: Fri, 01 Feb 2008 11:18:26 -0800 > To: > Subject: Re: [Histonet] Re: Histonet Digest, Vol 51, Issue 2 > > I'll try to formulate a brief answer to the question of validating > antibodies. If you are doing IHC both CLIA and CAP have regulations that > involve validation of antibodies. These regulations cover Establishment > and > Verification of Method Performance Specifications. The validation process > is designed to confirm the ability of the antibody to recognize the target > antigen in normal and diseased tissues where it is reasonably expected to > localize. And that there is not any obvious unexpected expression; in > other words, to determine its specificity and sensitivity. > To validate antibodies for IHC it's a multiple step process. First you > would > need to determine the working titer, pretreatment, detection & chromogen > that you are going to use. Use your standard SOPS. Use a control that > contains the antigen in question plus some negative elements. You don't > want > a control that is a high expressor or too low of an expresser at this > point. > Once you?ve determined your working parameters, you need to test the > antibody on normal tissue & diseased tissue that does & does not contain > the > antigen. For example, if you are validating an antibody that is positive in > Lung carcinomas you would want to use lung carcinomas (should be positive), > & a variety of carcinomas, lymphomas, etc... That should be negative. You > may find that this antibody is positive in GI carcinoma, too & you would > want to document that & know what % of GI carcinomas are positive, Also, > run > some normal tissues & assess their positive & negative rate. W compare our > findings with the published literature. You need to document all this work > ( > if you don?t document it, it didn?t happen). Excel or some other software > is > good for documenting your findings. We have a template that we use for all > work ups (& a validation SOP). > Hope this helps. Good luck. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > > > > > > does anyone validate antibody and how do you = do it? > > _________________________________________________________________ > > > > > > > > > > > > > > > > Con= fidentiality Notice: This email message, including any > > attachments, is for = the sole use of the intended recipient(s) and > > may contain confidential and = privileged information. Any > > unauthorized review, use, disclosure or distr= ibution is > > prohibited. If you are not the intended recipient, please cont= act > > the sender by reply email and destroy all copies of the original > > messag= e. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------ End of Forwarded Message > > > > This e-mail message, including any attachments, is for the sole use of the > intended recipients and may contain privileged information. Any > unauthorized > review, use, disclosure or distribution is prohibited. If you are not the > intended > recipient, please contact the sender by e-mail and destroy all copies of > the > original message, or you may call PhenoPath Laboratories, Seattle, WA > U.S.A. > at (206) 374-9000. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tracy.bergeron <@t> biogenidec.com Thu Oct 23 15:02:27 2008 From: tracy.bergeron <@t> biogenidec.com (Tracy Bergeron) Date: Thu Oct 23 15:03:57 2008 Subject: [Histonet] Tracy Bergeron is out of the office. Message-ID: I will be out of the office starting 10/22/2008 and will not return until 10/27/2008. I will respond to your message when I return. From histotechkb <@t> gmail.com Thu Oct 23 15:10:53 2008 From: histotechkb <@t> gmail.com (Kristen Yaros) Date: Thu Oct 23 15:10:58 2008 Subject: [Histonet] Histotechnology Society of Delaware - Fall Open Meeting and Hayride/Bonfire Message-ID: <667c97ab0810231310j1feb02f2y563f90dd955f523@mail.gmail.com> This is a reminder to all Delaware members and non-members that we will be holding our open meeting and Hayride/Bonfire at Carousel Park this Saturday, October, 25th from 3:00 to 6:00 pm. 3:00 Food and Open Meeting 4:00 Hayride 5:00 Bonfire and Smore's HSD is providing the main food. Everyone is asked to bring a covered side dish and fold up chair. Please RSVP ASAP to me! -- Kristen Yaros, HT (ASCP)CM Histotechnology Society of Delaware Correspondence Secretary histotechkb@gmail.com From bsmith <@t> bionix.com Thu Oct 23 15:13:53 2008 From: bsmith <@t> bionix.com (Smith, Brett) Date: Thu Oct 23 15:13:57 2008 Subject: [Histonet] Histology Lab Survey Invitation Message-ID: <7C42F4B987F67B4EA7AAD3633871A1900EE53E@bxsvr01.bionix.local> I represent a medical device company researching a concern in the histology lab and would like to invite you all to participate in a short, 2 - 4 minute survey. This survey will help verify a potential product need or will help prove that the need for a product does not exist. Please click on the following link to participate. http://www.bionix.com/Surveys/Slide_Contamination/hstscope.htm Thank you for your time. I look forward to your responses. Best Regards, Brett Brett Smith Marketing Coordinator Bionix Development Corporation Phone 419.727.8421 x241 Fax 419.727.8426 bsmith@bionix.com www.Bionix.com _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ EMAIL CONFIDENTIALITY NOTICE This Email message, and any attachments, may contain confidential patient health information that is legally protected. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this message is strictly prohibited. If you have received this information in error, please notify the sender immediately by replying to this message and delete the message from your system. From AGrobe2555 <@t> aol.com Thu Oct 23 16:11:13 2008 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Thu Oct 23 16:11:22 2008 Subject: [Histonet] need help for cultured cells Message-ID: You'll need to resuspend your pellet in a limited volume, do a cell count (using a hemocytometer, etc) and dilute the original suspension to the desired cell#/ul concentration with an appropriate diluent. Albert Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana Foundation **************Play online games for FREE at Games.com! All of your favorites, no registration required and great graphics ? check it out! (http://pr.atwola.com/promoclk/100000075x1211202682x1200689022/aol?redir= http://www.games.com?ncid=emlcntusgame00000001) From rachelr <@t> mail.nih.gov Thu Oct 23 18:51:33 2008 From: rachelr <@t> mail.nih.gov (Rivka Rachel) Date: Thu Oct 23 18:51:45 2008 Subject: [Histonet] Lipid stain for mammalian photoreceptors Message-ID: Is anyone aware of a lipid (or other) stain that would label the outer segments in mammalian photoreceptors in fixed tissue? We are aware of PPD which works well in plastic embedded tissue and are looking for something similar that would work with either paraffin embedded or cryosectioned tissue. -- Rivka A. Rachel, MD, PhD Staff Scientist, National Eye Institute Neurobiology, Neurodegeneration & Repair Laboratory Bldg 10 Rm 10D43 Tel: 301 443-4906 From ernestinemiddleton <@t> yahoo.ca Thu Oct 23 20:21:36 2008 From: ernestinemiddleton <@t> yahoo.ca (Ernestine Middleton) Date: Thu Oct 23 20:21:39 2008 Subject: [Histonet] microtome Message-ID: <897371.85745.qm@web51511.mail.re2.yahoo.com> Need suggestion on purchase a microtome that is good for section undecalified bone.? Please email me at : emiddlet@montefiore.org.? Thank. ? Ernestine Middleton Montefiiore Med. Ct. Bronx, NY l0467 718-920 4157 ? I using Xpress rapid processor.? I am experience problem with using Warthin starry for HP on the Aristan special stainer.? Anyone in histoland experience this problem. __________________________________________________________________ Be smarter than spam. See how smart SpamGuard is at giving junk email the boot with the All-new Yahoo! Mail. Click on Options in Mail and switch to New Mail today or register for free at http://mail.yahoo.ca From tifei <@t> foxmail.com Fri Oct 24 02:17:26 2008 From: tifei <@t> foxmail.com (tf) Date: Fri Oct 24 02:19:49 2008 Subject: [Histonet] BrdU labeling & TUNEL staining Message-ID: <200810241517208381208@foxmail.com> Dear All Do BrdU label nucleus disrupted cells? So that we have to do TUNEL as well? ANy references? I know that some people do this, but I am not clear about why. Another question is how to eliminate the background in BrdU staining. Especially the olfactory bulb, it has a high background in IHC. 2008-10-24 tf From maa8 <@t> cornell.edu Fri Oct 24 03:46:59 2008 From: maa8 <@t> cornell.edu (Mary A. Ascenzi) Date: Fri Oct 24 03:49:09 2008 Subject: [Histonet] muscle fibers Message-ID: Hi Patsy - the best one stop shop for info on muscle fiber is in Carson's book on Enzyme Histochemistry - CH13. It covers introduction on muscle fiber types, stains, and various techniques for identifying fast and slow twitch fibers. Judy at U Washington On Mon, 25 Aug 2008, Patsy Ruegg wrote: >Are there muscle experts out there that can help with this project? > > > >"Do you have any leads on differentiating and staining muscle fiber types? >We have mixed fiber type whole muscle embedded in paraffin and want to tell >if there is a difference between rats in the % distribution of muscle types. >They are sometimes called fast twitch and slow twitch muscles. Any leads >you could offer would be appreciated." > > > >Best regards, > >Patsy > > > > > >Patsy Ruegg, HT(ASCP)QIHC >IHCtech, LLC >Fitzsimmons BioScience Park >12635 Montview Blvd. Suite 215 >Aurora, CO 80010 >P-720-859-4060 >F-720-859-4110 >wk email pruegg@ihctech.net >web site www.ihctech.net > > > > >This email is confidential and intended solely for the use of the Person(s) >('the intended recipient') to whom it was addressed. Any views or opinions >presented are solely those of the author. It may contain information that is >privileged & confidential within the meaning of applicable law. Accordingly >any dissemination, distribution, copying, or other use of this message, or >any of its contents, by any person other than the intended recipient may >constitute a breach of civil or criminal law and is strictly prohibited. If >you are NOT the intended recipient please contact the sender and dispose of >this e-mail as soon as possible. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Fri Oct 24 09:10:25 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Fri Oct 24 09:10:33 2008 Subject: [Histonet] Histotechnology Society of Delaware - Fall Open Meeting and Hayride/Bonfire In-Reply-To: <667c97ab0810231310j1feb02f2y563f90dd955f523@mail.gmail.com> References: <667c97ab0810231310j1feb02f2y563f90dd955f523@mail.gmail.com> Message-ID: <946DCB339231F9106D9D2805@bchwxp2702.ad.med.buffalo.edu> We need this kind of Society in Buffalo, NY. This sounds like too much fun. --On Thursday, October 23, 2008 4:10 PM -0400 Kristen Yaros wrote: > This is a reminder to all Delaware members and non-members that we will be > holding our open meeting and Hayride/Bonfire at Carousel Park this > Saturday, October, 25th from 3:00 to 6:00 pm. > > 3:00 Food and Open Meeting > 4:00 Hayride > 5:00 Bonfire and Smore's > > HSD is providing the main food. Everyone is asked to bring a covered side > dish and fold up chair. > > Please RSVP ASAP to me! > > > -- > Kristen Yaros, HT (ASCP)CM > Histotechnology Society of Delaware > Correspondence Secretary > histotechkb@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (packages) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 "Without my flaws I'm really very boring." - random internet blog commentator From PMonfils <@t> Lifespan.org Fri Oct 24 09:21:19 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Oct 24 09:21:32 2008 Subject: [Histonet] Celestine Blue In-Reply-To: <4900581E.8FD5.00E5.0@medinst.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C68@LSRIEXCH1.lsmaster.lifespan.org> Try this place: http://www.rowleybio.com/ From relia1 <@t> earthlink.net Fri Oct 24 09:59:13 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Oct 24 09:59:20 2008 Subject: [Histonet] Heads Up on New Opportunities with RELIA Message-ID: Hi Histonetters!! Just wanted to drop a quick note about some new opportunities that I am working on. I hope everyone is looking forward to an awesome weekend enjoying Fall activites and the beautiful weather! I have new management positions in Cincinatti, OH, Austin, TX, Los Angeles, CA, Portland, OR and Manchester, NH I also have new histotech positions in San Francisco, Seattle, Dallas and Washington DC. I am also working with some of the best clients in Pittsburgh, Spokane, Seattle, Los Angeles, Dallas Baltimore and Washington, DC. If you or anyone you know would be interested in hearing more about any of these positions please contact me. I can be reached toll free at 866-607-3542 or at relia1@earthlink.net Remember I offer over 20 years of recruiting and employment counseling experience, knowledgeable, confidential and responsive service to you and your friends and a permanent placement practice dedicated to the histology profession. Have a Great Weekend!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net <> www.myspace.com/pamatrelia From tanisha.mcknight <@t> covance.com Fri Oct 24 10:26:17 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Fri Oct 24 10:26:39 2008 Subject: [Histonet] PAS with Glycogen Digestion - Solution Not Working Message-ID: <816E3C72F855F14985FC31D7C963AE6F0AE51629@indexch03.ent.covance.com> Hi All: I am in the process of working up a PAS Staining protocol. The PAS stain is working fine, but I am unable to get my tissue to digest. I am using .5g of Diastase in 50ml of DI water. I tried heating the solution in a water bath for 30 min, 1 hour and 1.5 hours and it still looks the same as the one without digestion. Could I be using the wrong Diastase? If so, does anyone have any suggestions for a new product? Thanks, Tanisha Neely, HT(ASCP) AP-Histology/Specimen Management ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From slappycraw <@t> yahoo.com Fri Oct 24 10:31:58 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Oct 24 10:32:08 2008 Subject: [Histonet] Question about cell buttons Message-ID: <895933.78438.qm@web53601.mail.re2.yahoo.com> Does anyone out there know what the material is that is used to make cell buttons in cytology and where to order it. Thanks in advance. ? Larry A. Woody Seattle, Wa. From rjbuesa <@t> yahoo.com Fri Oct 24 10:38:10 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 24 10:38:14 2008 Subject: [Histonet] PAS with Glycogen Digestion - Solution Not Working In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F0AE51629@indexch03.ent.covance.com> Message-ID: <981878.53101.qm@web65702.mail.ac4.yahoo.com> Tanisha: Being an enzyme, you cannot use diastase in distilled water. You have to prepare a diastase buffer at pH7 to dissolve the diastase and do the digestion at 37?C during 30 minutes. That will work. Please, do not try to use saliva! Ren? J. --- On Fri, 10/24/08, McKnight, Tanisha wrote: From: McKnight, Tanisha Subject: [Histonet] PAS with Glycogen Digestion - Solution Not Working To: histonet@lists.utsouthwestern.edu Date: Friday, October 24, 2008, 11:26 AM Hi All: I am in the process of working up a PAS Staining protocol. The PAS stain is working fine, but I am unable to get my tissue to digest. I am using .5g of Diastase in 50ml of DI water. I tried heating the solution in a water bath for 30 min, 1 hour and 1.5 hours and it still looks the same as the one without digestion. Could I be using the wrong Diastase? If so, does anyone have any suggestions for a new product? Thanks, Tanisha Neely, HT(ASCP) AP-Histology/Specimen Management ? From brett_connolly <@t> merck.com Fri Oct 24 10:41:12 2008 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Oct 24 10:41:14 2008 Subject: [Histonet] MAOB IHC on either frozen or FFPE sections Message-ID: <63EA0607835FBA4689CEA9EA8B4826920173B16B@usctmx1141.merck.com> Has anyone done it? I have an antibody from Abnova. They illustrate staining of FFPE kidney at 3ug/mL, but when I asked what the stock Ab concentration is they say they never determined that. Go figure? I'm looking for a starting point dilution. Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From leiker <@t> buffalo.edu Fri Oct 24 11:04:29 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Fri Oct 24 11:04:34 2008 Subject: [Histonet] MAOB IHC on either frozen or FFPE sections In-Reply-To: <63EA0607835FBA4689CEA9EA8B4826920173B16B@usctmx1141.merck.com> References: <63EA0607835FBA4689CEA9EA8B4826920173B16B@usctmx1141.merck.com> Message-ID: I've never worked with MAOB, but what I do when I run into similar situations is to do a 1:50 and 1:200 dilution in parallel to start getting a feel for the antibody. (Make sure to use antigen retrieval if you need it on the FFPE sections; apologies if you already knew that). Yeah I, too, think it's weird that they illustrate the antibody at a particular concentration and then tell you they never determined the stock concentration! Merced --On Friday, October 24, 2008 11:41 AM -0400 "Connolly, Brett M" wrote: > Has anyone done it? I have an antibody from Abnova. They illustrate > staining of FFPE kidney at 3ug/mL, but when I asked what the stock Ab > concentration is they say they never determined that. Go figure? I'm > looking for a starting point dilution. > Thanks, > Brett > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Research > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > PH 215-652-2501 fax. 215-993-6803 > e-mail. brett_connolly@merck.com > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates (which may be known > outside the United States as Merck Frosst, Merck Sharp & Dohme or > MSD and in Japan, as Banyu - direct contact information for affiliates is > available at http://www.merck.com/contact/contacts.html) that may be > confidential, proprietary copyrighted and/or legally privileged. It is > intended solely for the use of the individual or entity named on this > message. If you are not the intended recipient, and have received this > message in error, please notify us immediately by reply e-mail and > then delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (packages) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 "Without my flaws I'm really very boring." - random internet blog commentator From ian.montgomery <@t> bio.gla.ac.uk Fri Oct 24 11:32:10 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Oct 24 11:34:28 2008 Subject: [Histonet] Bones Message-ID: <5AFF13ACD118476CB3E10EC0F4001119@IBLS.GLA.AC.UK> I have some bones, from various species, that I want to clean of muscle, tendons, etc, etc. The method currently used is boiling the bones in water for several hours, days until they are completely clean. Problem, it's a wee bit smelly, in fact a big bit smelly. Me being a delicate soul more used to various exotic eau de parfum wonder if there is another technique available. Some species respond to soaking for several weeks in laboratory detergent while others don't. NaOH or KOH, again some do others don't. What I would ideally like is a universal method that's reasonably quick, but not smelly, can anyone help. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From johnsobr <@t> zgi.com Fri Oct 24 11:57:24 2008 From: johnsobr <@t> zgi.com (BJON (Brian Johnson)) Date: Fri Oct 24 11:57:44 2008 Subject: [Histonet] Human anti Human Message-ID: <746E68D5CBB205409B12EC32A61EE4010A2FE8E7@ned.zgi.com> Hello I am wondering if anyone has found, and or used, an IHC polymer kit for a human anti human monoclonal antibody. Beyond the polymer kit, does anyone have any tips for using a human anti human antibody for IHC without biotinylating it? Thank you. Brian Johnson From SwainFrancesL <@t> uams.edu Fri Oct 24 11:51:26 2008 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Fri Oct 24 11:58:22 2008 Subject: [Histonet] RE: [IHCRG] MAOB IHC on either frozen or FFPE sections In-Reply-To: <63EA0607835FBA4689CEA9EA8B4826920173B16B@usctmx1141.merck.com> References: <63EA0607835FBA4689CEA9EA8B4826920173B16B@usctmx1141.merck.com> Message-ID: Hi bert ifyou will look at the image for the antibody for IHC of MAOB you will see at the bottom that the antibody demonstrated was at a concentration of 3 ug/ml Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email ________________________________ From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Connolly, Brett M Sent: Friday, October 24, 2008 10:41 AM To: ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: [IHCRG] MAOB IHC on either frozen or FFPE sections Has anyone done it? I have an antibody from Abnova. They illustrate staining of FFPE kidney at 3ug/mL, but when I asked what the stock Ab concentration is they say they never determined that. Go figure? I'm looking for a starting point dilution. Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From leiker <@t> buffalo.edu Fri Oct 24 12:09:43 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Fri Oct 24 12:09:49 2008 Subject: [Histonet] RE: [IHCRG] MAOB IHC on either frozen or FFPE sections In-Reply-To: References: <63EA0607835FBA4689CEA9EA8B4826920173B16B@usctmx1141.merck.com> Message-ID: <9BE1479BDCDACB477C0F4094@bchwxp2702.ad.med.buffalo.edu> 3ug/ml sounds awful low for a stock concentration... --On Friday, October 24, 2008 11:51 AM -0500 "Swain, Frances L" wrote: > Hi bert ifyou will look at the image for the antibody for IHC of MAOB you > will see at the bottom that the antibody demonstrated was at a > concentration of 3 ug/ml > > > > Frances L. Swain HT(ASCP) A. A. S. > > Special Procedures Technician > > Department of Orthopaedic Surgery > > Center for Orthopaedic Research > > Barton Research Building 2R28 > > 4301 West Markham Street > > Little Rock AR 72205 > > (501) 686-8739 PHONE > > (501) 686-8987 FAX > > swainfrancesl@uams.edu email > > ________________________________ > > From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of > Connolly, Brett M > Sent: Friday, October 24, 2008 10:41 AM > To: ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu > Subject: [IHCRG] MAOB IHC on either frozen or FFPE sections > > > > Has anyone done it? I have an antibody from Abnova. They illustrate > staining of FFPE kidney at 3ug/mL, but when I asked what the stock Ab > concentration is they say they never determined that. Go figure? I'm > looking for a starting point dilution. > > Thanks, > Brett > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Research > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > PH 215-652-2501 fax. 215-993-6803 > e-mail. brett_connolly@merck.com > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates (which may be known > outside the United States as Merck Frosst, Merck Sharp & Dohme or > MSD and in Japan, as Banyu - direct contact information for affiliates is > available at http://www.merck.com/contact/contacts.html) that may be > confidential, proprietary copyrighted and/or legally privileged. It is > intended solely for the use of the individual or entity named on this > message. If you are not the intended recipient, and have received this > message in error, please notify us immediately by reply e-mail and > then delete it from your system. > > > --~--~---------~--~----~------------~-------~--~----~ > You received this message because you are subscribed to the Google Groups > "ihcrg" group. > To post to this group, send email to ihcrg@googlegroups.com > To unsubscribe from this group, send email to > ihcrg+unsubscribe@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > -~----------~----~----~----~------~----~------~--~--- > > > > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by reply e-mail and destroy all > copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (packages) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 "Without my flaws I'm really very boring." - random internet blog commentator From kbowden <@t> ucsd.edu Fri Oct 24 12:12:54 2008 From: kbowden <@t> ucsd.edu (kbowden) Date: Fri Oct 24 12:13:15 2008 Subject: [Histonet] Bones In-Reply-To: <5AFF13ACD118476CB3E10EC0F4001119@IBLS.GLA.AC.UK> References: <5AFF13ACD118476CB3E10EC0F4001119@IBLS.GLA.AC.UK> Message-ID: <49020216.3030108@ucsd.edu> One of the departments (body donations) here uses bugs to clean soft tissue off of bones. I thinks it takes about a week or so. You might look into the type of bugs you have in your region for that purpose. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. Ian Montgomery wrote: > I have some bones, from various species, that I want to clean of > muscle, tendons, etc, etc. The method currently used is boiling the bones in > water for several hours, days until they are completely clean. Problem, it's > a wee bit smelly, in fact a big bit smelly. Me being a delicate soul more > used to various exotic eau de parfum wonder if there is another technique > available. Some species respond to soaking for several weeks in laboratory > detergent while others don't. NaOH or KOH, again some do others don't. What > I would ideally like is a universal method that's reasonably quick, but not > smelly, can anyone help. > > Ian. > > > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From leiker <@t> buffalo.edu Fri Oct 24 12:29:12 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Fri Oct 24 12:29:17 2008 Subject: [Histonet] RE: [IHCRG] MAOB IHC on either frozen or FFPE sections In-Reply-To: <9BE1479BDCDACB477C0F4094@bchwxp2702.ad.med.buffalo.edu> References: <63EA0607835FBA4689CEA9EA8B4826920173B16B@usctmx1141.merck.com> <9BE1479BDCDACB477C0F4094@bchwxp2702.ad.med.buffalo.edu> Message-ID: <5F97EE6FF4071D4CBEDF6C67@bchwxp2702.ad.med.buffalo.edu> ...considering the stock Ab concentration isn't even known, as Brett mentioned. --On Friday, October 24, 2008 1:09 PM -0400 Merced Leiker wrote: > > 3ug/ml sounds awful low for a stock concentration... > > > --On Friday, October 24, 2008 11:51 AM -0500 "Swain, Frances L" > wrote: > >> Hi bert ifyou will look at the image for the antibody for IHC of MAOB you >> will see at the bottom that the antibody demonstrated was at a >> concentration of 3 ug/ml >> >> >> >> Frances L. Swain HT(ASCP) A. A. S. >> >> Special Procedures Technician >> >> Department of Orthopaedic Surgery >> >> Center for Orthopaedic Research >> >> Barton Research Building 2R28 >> >> 4301 West Markham Street >> >> Little Rock AR 72205 >> >> (501) 686-8739 PHONE >> >> (501) 686-8987 FAX >> >> swainfrancesl@uams.edu email >> >> ________________________________ >> >> From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of >> Connolly, Brett M >> Sent: Friday, October 24, 2008 10:41 AM >> To: ihcrg@googlegroups.com; histonet@lists.utsouthwestern.edu >> Subject: [IHCRG] MAOB IHC on either frozen or FFPE sections >> >> >> >> Has anyone done it? I have an antibody from Abnova. They illustrate >> staining of FFPE kidney at 3ug/mL, but when I asked what the stock Ab >> concentration is they say they never determined that. Go figure? I'm >> looking for a starting point dilution. >> >> Thanks, >> Brett >> >> Brett M. Connolly, Ph.D. >> Research Fellow, Imaging Research >> Merck & Co., Inc. >> PO Box 4, WP-44K >> West Point, PA 19486 >> PH 215-652-2501 fax. 215-993-6803 >> e-mail. brett_connolly@merck.com >> >> Notice: This e-mail message, together with any attachments, contains >> information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, >> New Jersey, USA 08889), and/or its affiliates (which may be known >> outside the United States as Merck Frosst, Merck Sharp & Dohme or >> MSD and in Japan, as Banyu - direct contact information for affiliates is >> available at http://www.merck.com/contact/contacts.html) that may be >> confidential, proprietary copyrighted and/or legally privileged. It is >> intended solely for the use of the individual or entity named on this >> message. If you are not the intended recipient, and have received this >> message in error, please notify us immediately by reply e-mail and >> then delete it from your system. >> >> >> --~--~---------~--~----~------------~-------~--~----~ >> You received this message because you are subscribed to the Google Groups >> "ihcrg" group. >> To post to this group, send email to ihcrg@googlegroups.com >> To unsubscribe from this group, send email to >> ihcrg+unsubscribe@googlegroups.com >> For more options, visit this group at >> http://groups.google.com/group/ihcrg?hl=en >> -~----------~----~----~----~------~----~------~--~--- >> >> >> >> Confidentiality Notice: This e-mail message, including any attachments, >> is for the sole use of the intended recipient(s) and may contain >> confidential and privileged information. Any unauthorized review, use, >> disclosure or distribution is prohibited. If you are not the intended >> recipient, please contact the sender by reply e-mail and destroy all >> copies of the original message. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > 354 BRB (packages) / 140 Farber Hall (mail) > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > "Without my flaws I'm really very boring." > - random internet blog commentator > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (packages) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 "Without my flaws I'm really very boring." - random internet blog commentator From soofias2 <@t> yahoo.com Fri Oct 24 13:10:05 2008 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Fri Oct 24 13:10:09 2008 Subject: [Histonet] Need help for CD95(FAS) and antigen retrieval system Message-ID: <291774.83461.qm@web90506.mail.mud.yahoo.com> Hi everyone, I am sure some one in this large world of histonet will be able to help me. I am trying to do CD95 staining on human skin FF PE tissue sections. CD95 is 45 KD single chain type cell surface??protein that mediates apoptosis when crosslinked with agnostic anti-Fas antibodies.I have tried citric acid pH 6.0 buffer in microwave and and 99 degree Celsius?water bath incubator and also tried sodium citrate Buffer pH 6.00 on microwave and incubator and also?Trypsin for antigen retrieval, But I could not get any success. I am using R&D MAB 142 monoclonal antibody Clone DX2 and also using BioLegend 3056130 monoclonal antibody clone DX2, but none of the method worked to get the signaling. Please provide me,?if?? you ?have some suggestion or any better antigen retrieval method for Fas antibodies. Thank you in advance.? Soofia UWHC,Madison, WI? From RSRICHMOND <@t> aol.com Fri Oct 24 13:30:29 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Oct 24 13:30:35 2008 Subject: [Histonet] Re: Question about cell buttons Message-ID: Larry A. Woody in Seattle WA asks: >>Does anyone out there know what the material is that is used to make cell buttons in cytology and where to order it?<< I suppose you're referring to the embedding gel for cytology cell blocks (often done with pleural and peritoneal fluids). It goes by the trade name of HistoGel. I think it's made by whatever Thermo is called this week. There's a considerable amount of information about it in the Histonet archives - check Google. An alternative is 3% agar, such as tubed TSB (trypticase soy broth) if they still make that. I don't have a lot of experience with either of these materials. I have no commercial connection with any of these products. Bob Richmond Samurai Pathologist Knoxville TN From JWeems <@t> sjha.org Fri Oct 24 13:33:13 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Oct 24 13:33:26 2008 Subject: [Histonet] Productivity Message-ID: <5D64396A0D4A5346BEBC759022AAEAA50E3DFC@ITSSSXM01V6.one.ads.che.org> For those involved with Cytology, would you please reply to me privately... We have one full time Cytology Tech Specialist and 0.5 lab assistant for cyto prep. We are a low volume non-gyn lab (2500 cases YTD) - totally ThinPrep except for thyroid aspirates that come from one practice with up to 5 extra smeared slides. Workload has decreased and is sporadic. The extra time could definitely be used for manual preparation, CAP prep, QC/QA, etc. I've been asked to decrease lab asst hours, but tech says TAT would not be met if that happens. Would you all share your process with me? How much is expected from your Cytotechs? Thanks very much - have a good weekend everybody! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From SCOTT.TURNER <@t> SPCORP.COM Fri Oct 24 13:35:52 2008 From: SCOTT.TURNER <@t> SPCORP.COM (Turner, Scott) Date: Fri Oct 24 13:36:08 2008 Subject: [Histonet] Human anti Human In-Reply-To: <746E68D5CBB205409B12EC32A61EE4010A2FE8E7@ned.zgi.com> Message-ID: <9A919A5D70313A4D9C56A025710874080313D7D3@kenmsg40.us.schp.com> Molecular Probes (now Invitrogen) makes some really good kits for doing fluorescent labeling of human antibodies that can be applied to human tissue. They also make kits for mouse on mouse applications. These Zenon kits come in a variety of fluorphores and I believe they also have some haptens like biotin that can be used for chromagenic staining. It is essentially the same concept as the Dako Animal Research Kit: pre-labeling the primary mAb with a labeled Fab fragment secondary followed by an Ig block to absorb the unbound secondary antibody. Scott Turner Scientist II Schering-Plough Biopharma Palo Alto, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of BJON (Brian Johnson) Sent: Friday, October 24, 2008 09:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Human anti Human Hello I am wondering if anyone has found, and or used, an IHC polymer kit for a human anti human monoclonal antibody. Beyond the polymer kit, does anyone have any tips for using a human anti human antibody for IHC without biotinylating it? Thank you. Brian Johnson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From CHRISH <@t> HEALTHCARESCOUTS.COM Fri Oct 24 13:34:33 2008 From: CHRISH <@t> HEALTHCARESCOUTS.COM (Chris Handrahan) Date: Fri Oct 24 13:42:09 2008 Subject: [Histonet] Histology Openings in Dallas Message-ID: Looking for ASCP certified Histotechs for the following openings in Dallas day position, 8a-5:00pm 2nd shift position, 4pm - 12:30am 2nd shift position, 6pm - 2:00am 3rd shift position, 1am - 9:00am These are all full time permanent positions. These are immediate needs so please contact me today if you are interested! Have a great weekend! Chris Handrahan Managing Director of Allied Health Healthcare Scouts 800-708-0605 office 321-231-5427 cell chrish@healthcarescouts.com www.healthcarescouts.com From juditw <@t> u.washington.edu Fri Oct 24 13:55:44 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Fri Oct 24 13:55:50 2008 Subject: [Histonet] Bones In-Reply-To: <49020216.3030108@ucsd.edu> Message-ID: Hi Ian and all histonetters- The bugs are called Dermestid beetles. Most Museums use them. Once the colony you have is going - the can demeat any bone in 24hrs just about! they are clean, neat and do not smell. You can order them from Carolina Biological supply in North carolina, USA. probably other places too. If you know anyone in the museum antropology department, they may have some. Judy On Fri, 24 Oct 2008, kbowden wrote: > One of the departments (body donations) here uses bugs to clean soft tissue off > of bones. I thinks it takes about a week or so. You might look into the type > of bugs you have in your region for that purpose. > -- > Karen Bowden > Staff Research Associate II > University of CA, San Diego > Department of Orthopedics > 9500 Gilman Dr. 0630 > La Jolla, CA 92093-0630 > 858-534-4655 voice > 858-534-5304 fax > > > CONFIDENTIALITY NOTICE: > THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE > PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL > AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR > OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION > BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. > IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND > DELETE THE MATERIAL FROM ANY COMPUTER. > > > Ian Montgomery wrote: >> I have some bones, from various species, that I want to clean of >> muscle, tendons, etc, etc. The method currently used is boiling the bones in >> water for several hours, days until they are completely clean. Problem, it's >> a wee bit smelly, in fact a big bit smelly. Me being a delicate soul more >> used to various exotic eau de parfum wonder if there is another technique >> available. Some species respond to soaking for several weeks in laboratory >> detergent while others don't. NaOH or KOH, again some do others don't. What >> I would ideally like is a universal method that's reasonably quick, but not >> smelly, can anyone help. >> >> Ian. Dr. Ian Montgomery, >> >> Histotechnology, >> >> I.B.L.S. Support Unit, >> >> Thomson Building, >> >> University of Glasgow, >> >> Glasgow, >> >> G12 8QQ. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From suhyoung.jeong <@t> gmail.com Fri Oct 24 15:02:24 2008 From: suhyoung.jeong <@t> gmail.com (Suhyoung Jeong) Date: Fri Oct 24 15:02:28 2008 Subject: [Histonet] More than 4 years old secondary antibodies Message-ID: <450012a20810241302q73d4769cq4293a93d9514ac00@mail.gmail.com> Hello everyone, I got some still-lyophilyzed kept-in-4C secondary antibodies recently. We don't know exactly how old they are, but the company (Zymed) could not track the lot number on the bottle. They said that they discard the record after 4 years and that makes these antibodies at least 4 years old. I assume between 5-7 years old. Has anyone have some idea whether it will be worth testing these antibodies? Thank you in advance Regards, Suh From leiker <@t> buffalo.edu Fri Oct 24 15:51:17 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Fri Oct 24 15:51:24 2008 Subject: [Histonet] Bones In-Reply-To: References: Message-ID: Wow! So many possible uses for those bugs come to mind...and it's so close to Halloween... ;-) --On Friday, October 24, 2008 11:55 AM -0700 "Judith L. Williams" wrote: > Hi Ian and all histonetters- > The bugs are called Dermestid beetles. Most Museums use them. Once the > colony you have is going - the can demeat any bone in 24hrs just about! > they are clean, neat and do not smell. You can order them from Carolina > Biological supply in North carolina, USA. probably other places too. If > you know anyone in the museum antropology department, they may have some. > Judy > > On Fri, 24 Oct 2008, kbowden wrote: > >> One of the departments (body donations) here uses bugs to clean soft >> tissue off of bones. I thinks it takes about a week or so. You might >> look into the type of bugs you have in your region for that purpose. >> -- >> Karen Bowden >> Staff Research Associate II >> University of CA, San Diego >> Department of Orthopedics >> 9500 Gilman Dr. 0630 >> La Jolla, CA 92093-0630 >> 858-534-4655 voice >> 858-534-5304 fax >> >> >> CONFIDENTIALITY NOTICE: >> THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE >> PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL >> AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR >> OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION >> BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. >> IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND >> DELETE THE MATERIAL FROM ANY COMPUTER. >> >> >> Ian Montgomery wrote: >>> I have some bones, from various species, that I want to >>> clean of muscle, tendons, etc, etc. The method currently >>> used is boiling the bones in water for several hours, days until they >>> are completely clean. Problem, it's a wee bit smelly, in fact a big bit >>> smelly. Me being a delicate soul more used to various exotic eau de >>> parfum wonder if there is another technique available. Some species >>> respond to soaking for several weeks in laboratory detergent while >>> others don't. NaOH or KOH, again some do others don't. What I would >>> ideally like is a universal method that's reasonably quick, but not >>> smelly, can anyone help. >>> >>> Ian. Dr. Ian Montgomery, >>> >>> Histotechnology, >>> >>> I.B.L.S. Support Unit, >>> >>> Thomson Building, >>> >>> University of Glasgow, >>> >>> Glasgow, >>> >>> G12 8QQ. >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > Judith Williams, PhD, HT(ASCP) > Research Scientist > Department of Comparative Medicine > University of Washington > Seattle, WA 98195 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (packages) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 "Without my flaws I'm really very boring." - random internet blog commentator From renafail <@t> bellsouth.net Fri Oct 24 20:47:53 2008 From: renafail <@t> bellsouth.net (renafail@bellsouth.net) Date: Fri Oct 24 20:47:57 2008 Subject: [Histonet] PAS with Glycogen Digestion - Solution Not Working In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F0AE51629@indexch03.ent.covance.com> Message-ID: <102520080147.18695.49027AC8000B1B760000490722218683269B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net> I had far more consistent results with amylase type VI (sigma) than diastase, recommended by someone from histonet when I had problems with diastase Ren Fail -------------- Original message from "McKnight, Tanisha" : -------------- > Hi All: > > I am in the process of working up a PAS Staining protocol. The PAS stain > is working fine, but I am unable to get my tissue to digest. I am using > .5g of Diastase in 50ml of DI water. I tried heating the solution in a > water bath for 30 min, 1 hour and 1.5 hours and it still looks the same > as the one without digestion. Could I be using the wrong Diastase? If > so, does anyone have any suggestions for a new product? > > Thanks, > > Tanisha Neely, HT(ASCP) > AP-Histology/Specimen Management > > > > > > ----------------------------------------------------- > Confidentiality Notice: This e-mail transmission > may contain confidential or legally privileged > information that is intended only for the individual > or entity named in the e-mail address. If you are not > the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or reliance > upon the contents of this e-mail is strictly prohibited. > > If you have received this e-mail transmission in error, > please reply to the sender, so that we can arrange > for proper delivery, and then please delete the message > from your inbox. Thank you. > From llewllew <@t> shaw.ca Fri Oct 24 21:54:09 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Oct 24 21:54:40 2008 Subject: Fw: [Histonet] PAS with Glycogen Digestion - Solution Not Working Message-ID: ----- Original Message ----- From: "Bryan Llewellyn" To: "McKnight, Tanisha" Sent: Friday, October 24, 2008 9:34 AM Subject: Re: [Histonet] PAS with Glycogen Digestion - Solution Not Working > Try porcine pancreatic alpha amylase from Sigma (A 3176). I used the > amount to cover a dime 1 mm deep in 15 mL distilled water and it always > worked for me in an hour at room temperature. > > However, in general, you should base your concentration on the amount of > enzyme you need rather than the powder that contains the enzyme. That is > 0.5 grams of a 10 units per gram powder is not the same concentration as > 0.5 grams of a 100 units per gram powder. > > Bryan Llewellyn > > ----- Original Message ----- > From: "McKnight, Tanisha" > To: > Sent: Friday, October 24, 2008 8:26 AM > Subject: [Histonet] PAS with Glycogen Digestion - Solution Not Working > > >> Hi All: >> >> I am in the process of working up a PAS Staining protocol. The PAS stain >> is working fine, but I am unable to get my tissue to digest. I am using >> .5g of Diastase in 50ml of DI water. I tried heating the solution in a >> water bath for 30 min, 1 hour and 1.5 hours and it still looks the same >> as the one without digestion. Could I be using the wrong Diastase? If >> so, does anyone have any suggestions for a new product? >> >> Thanks, >> >> Tanisha Neely, HT(ASCP) >> AP-Histology/Specimen Management >> >> >> >> >> >> ----------------------------------------------------- >> Confidentiality Notice: This e-mail transmission >> may contain confidential or legally privileged >> information that is intended only for the individual >> or entity named in the e-mail address. If you are not >> the intended recipient, you are hereby notified that >> any disclosure, copying, distribution, or reliance >> upon the contents of this e-mail is strictly prohibited. >> >> If you have received this e-mail transmission in error, >> please reply to the sender, so that we can arrange >> for proper delivery, and then please delete the message >> from your inbox. Thank you. >> >> > > > -------------------------------------------------------------------------------- > > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From anhtx <@t> drreddys.com Sat Oct 25 01:48:26 2008 From: anhtx <@t> drreddys.com (anhtx@drreddys.com) Date: Sat Oct 25 01:40:03 2008 Subject: [Histonet] mitotic figures staining Message-ID: Hello everyone This is Girish working as toxicologic pathologist for a drug discovery firm based in India. We are in the process of evaluating mitotic indices/figures in the liver sections of rat. Can anyone help regarding what kind of simple histotechniques are useful for this ? When we went through the literature, we could notice that Toluidine blue and Crystal violet staining will serve the purpose to differentiate from apoptotic bodies and pyknotic nuclei. However, we didn't get the staining protocol for either. If anybody has working experience on this, please share your views Regards and thanks Dr Girish B.Chandrashekar Scientist IB Preclinical Safety Evaluation Discovery Research Dr Reddy's Laboratories Hyderabad, India. Disclaimer This message contains legally privileged and/or confidential information. If you are not the intended recipient(s), or employee or agent responsible for delivery of this message to the intended recipient(s), you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this message in error, please immediately notify the sender and delete this e-mail message from your computer. WARNING: Computer viruses can be transmitted via email. The recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email. From gu.lang <@t> gmx.at Sat Oct 25 05:40:29 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Oct 25 05:41:10 2008 Subject: [Histonet] synonym of Factor VIII? Message-ID: Hi all, I need some help to find the right antibody for IHC. My pathologists told me to find a Factor VIIIa - antibody. It should be a good marker for angioendothels. When I search, always the "vonWillebrand Factor" appears. And I don't know, if that are synonyms for the same antigen. And if there even exists an VIII a, with stress on "a". Can anyone help me? Or even recommend an antibody (Ventana Benchmark XT)? Bye Gudrun Lang Histolabor, Akh Linz, Austria From tahseen <@t> brain.net.pk Sat Oct 25 06:04:55 2008 From: tahseen <@t> brain.net.pk (tahseen@brain.net.pk) Date: Sat Oct 25 06:05:04 2008 Subject: [Histonet] Protect fingers during Microtomy Message-ID: <55411.202.125.145.178.1224932695.squirrel@brain.net.pk> Hi Histoneters, I am looking some think (like gloves)to protect the fingers during Microtomy. Thanks, Muhammad Tahseen Histology supervisor SKMCH&RC Lahore,Pakistan From amosbrooks <@t> gmail.com Sat Oct 25 11:18:56 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Oct 25 11:19:02 2008 Subject: [Histonet] Human anti Human Message-ID: <582736990810250918n3fedac78rf9b08f2fba23256@mail.gmail.com> Hi, I should start by saying: I have not done this, but this is the way I would approach this problem if I were to encounter it. First I assume you have an human antibody raised in humans right? So, you need an antibody that will detect the human antibody like "Anti-Human IgG (?-chain specific) antibody produced in rabbit" or "Monoclonal Anti-Human IgG (?-chain specific) antibody produced in mouse". (As seen here: http://www.sigmaaldrich.com/life-science/cell-biology/antibodies/antibody-products.htm?TablePage=14574992. Then you can detect the Rabbit or Mouse secondary with your usual anti Rabbit or Mouse detection. (I would use a polymer just to minimize biotin background.) If anyone sees any holes in that line of approach, I'd love to hear other ideas. It's an interesting problem. Best of luck, Amos Brooks Message: 22 Date: Fri, 24 Oct 2008 09:57:24 -0700 From: "BJON (Brian Johnson)" Subject: [Histonet] Human anti Human To: histonet@lists.utsouthwestern.edu Message-ID: <746E68D5CBB205409B12EC32A61EE4010A2FE8E7@ned.zgi.com> Content-Type: text/plain; charset=us-ascii Hello I am wondering if anyone has found, and or used, an IHC polymer kit for a human anti human monoclonal antibody. Beyond the polymer kit, does anyone have any tips for using a human anti human antibody for IHC without biotinylating it? Thank you. Brian Johnson From renafail <@t> bellsouth.net Sat Oct 25 18:20:07 2008 From: renafail <@t> bellsouth.net (renafail@bellsouth.net) Date: Sat Oct 25 18:20:13 2008 Subject: [Histonet] synonym of Factor VIII? In-Reply-To: Message-ID: <102520082320.5135.4903A9A7000308B60000140F22230650629B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net> Factor VIII is also known as vonWillebrand factor. I don't recall a Factor VIIIa.though there may very be one. There is a however a Factor XIIIa not the same at all. Rena -------------- Original message from "Gudrun Lang" : -------------- > Hi all, > > I need some help to find the right antibody for IHC. My pathologists told me > to find a Factor VIIIa - antibody. It should be a good marker for > angioendothels. > > When I search, always the "vonWillebrand Factor" appears. And I don't know, > if that are synonyms for the same antigen. > > And if there even exists an VIII a, with stress on "a". > > > > Can anyone help me? Or even recommend an antibody (Ventana Benchmark XT)? > > Bye > > Gudrun Lang > > > > Histolabor, Akh Linz, Austria > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From epearlstein <@t> hotmail.com Sat Oct 25 20:31:45 2008 From: epearlstein <@t> hotmail.com (Ellen Pearlstein) Date: Sat Oct 25 20:31:50 2008 Subject: [Histonet] stainless steel perforated slides Message-ID: I am wondering whether anyone on the list knows of a source for stainless steel perforated microscope slides that could be used for sectioning plant and animal fibers? Thank you. Ellen Pearlstein UCLA _________________________________________________________________ When your life is on the go?take your life with you. http://clk.atdmt.com/MRT/go/115298558/direct/01/ From hilda_1075 <@t> yahoo.com Sat Oct 25 22:35:14 2008 From: hilda_1075 <@t> yahoo.com (shazana hilda) Date: Sat Oct 25 22:35:17 2008 Subject: [Histonet] IHC scoring system Message-ID: <288664.56177.qm@web38808.mail.mud.yahoo.com> Dear all, I've done the IHC staining step unfortunately encounter problem for a good scoring system (i.e scoring that balance between cell distribution and staining intensity). Hope anyone who have experience in this matter can share they're opinion. Thank you in advance. Cheers, Shazana Hilda Shamsuddin MSc.of Molecular Pathology, Dept. of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: hilda_1075@yahoo.com From gu.lang <@t> gmx.at Sun Oct 26 02:55:36 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Oct 26 02:55:59 2008 Subject: AW: [Histonet] synonym of Factor VIII? In-Reply-To: <102520082320.5135.4903A9A7000308B60000140F22230650629B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net> References: <102520082320.5135.4903A9A7000308B60000140F22230650629B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net> Message-ID: <69F96D41F9294BD09507A9517E3B3268@dielangs.at> Many thanks to all that answered my question, but I am still in doubt. This is from Wikipedia: "Von Willebrand factor is not an enzyme and therefore has no catalytic activity. Its primary function is binding to other proteins, particularly Factor VIII and it is important in platelet adhesion to wound sites". "Factor VIII is bound to vWF while inactive in circulation; Factor VIII degrades rapidly when not bound to vWF. Factor VIII is released from vWF by the action of thrombin." Could it be possible, that in our technical manner the two proteins are seen as one, because Factor VIII alone can hardly be detected? Gudrun Lang _____ Von: renafail@bellsouth.net [mailto:renafail@bellsouth.net] Gesendet: Sonntag, 26. Oktober 2008 00:20 An: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Cc: Gudrun Lang Betreff: Re: [Histonet] synonym of Factor VIII? Factor VIII is also known as vonWillebrand factor. I don't recall a Factor VIIIa.though there may very be one. There is a however a Factor XIIIa not the same at all. Rena -------------- Original message from "Gudrun Lang" : -------------- > Hi all, > > I need some help to find the right antibody for IHC. My pathologists told me > to find a Factor VIIIa - antibody. It should be a good marker for > angioendothels. > > When I search, always the "vonWillebrand Factor" appears. And I don't know, > if that are synonyms for the same antigen. > > And if there even exists an VIII a, with stress on "a". > > > > Can anyone help me? Or even recommend an antibody (Ventana Benchmark XT)? > > Bye > > Gudrun Lang > > > > Histolabor, Akh Linz, Austria > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mikael.niku <@t> helsinki.fi Sun Oct 26 04:03:14 2008 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Sun Oct 26 04:03:24 2008 Subject: [Histonet] getting rid of biotin-avidin background References: Message-ID: <007c01c93749$b00f68f0$2101a8c0@toukka> Dear Tyrone (and others), I'm routinely and happily using egg white powder (really raw avidin) and biotin (by Sigma) to block endogenous biotin, as suggested earlier on Histonet. Before primary ab (and perhaps also the serum block, doesn't really matter), incubate slides for 15 min in 10% (w/v) egg white powder in water. Wash. Then incubate in 0.1 mg/ml biotin in buffer for another 15 min (it's also possible to just add this to the primary ab). The solutions: Add 10% (w/v) egg white powder in water. Stir to solubilize most of the stuff. Store in the freezer (seems to be OK to thaw repeatedly). . Before use, spin briefly to get rid of the unsolubilized material. Biotin stock: 1 mg/ml D-biotin in your IHC buffer (you can find the exact Sigma product # elsewhere in Histonet, if necessary.. probably doesn't matter). Store in the freezer (OK to thaw/refreeze). Dilute 1/10 for use. Works quite nicely, though I think I never tried commercial blocking kits for comparison. I think the crude nature of the "avidin solution" is actually a bonus, adding a protein blocking effect. The most difficult part might be obtaining the egg white powder, which isn't available in grocery stores at least in Finland. After some searching, I got a huge bag of it from some industrial egg company. Enough for a few centuries of use and of course practically free (whereas purified avidin seems to be really expensive). With best regards, Mikael ----------------------------------------------------- Mikael Niku, PhD, university lecturer University of Helsinki, Division of Nutrition URL: mikael.nikunnakki.info - What do I think of western civilization? I think it would be a good idea! Gandhi ----------------------------------------------------- From Rcartun <@t> harthosp.org Sun Oct 26 08:33:06 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun Oct 26 08:33:22 2008 Subject: [Histonet] IHC scoring system In-Reply-To: <288664.56177.qm@web38808.mail.mud.yahoo.com> References: <288664.56177.qm@web38808.mail.mud.yahoo.com> Message-ID: <4904395202000077000066C3@gwmail4.harthosp.org> Use the "Allred" scoring system (see Mod Pathol 1998;11(2):155-168). Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> shazana hilda 10/25/08 11:35 PM >>> Dear all, I've done the IHC staining step unfortunately encounter problem for a good scoring system (i.e scoring that balance between cell distribution and staining intensity). Hope anyone who have experience in this matter can share they're opinion. Thank you in advance. Cheers, Shazana Hilda Shamsuddin MSc.of Molecular Pathology, Dept. of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: hilda_1075@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun Oct 26 08:40:24 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Oct 26 08:40:28 2008 Subject: [Histonet] IHC scoring system In-Reply-To: <288664.56177.qm@web38808.mail.mud.yahoo.com> Message-ID: <602793.71176.qm@web65715.mail.ac4.yahoo.com> Hilda: Under separate cover I am sending an article I wrote on this very same subject. Ren? J. --- On Sat, 10/25/08, shazana hilda wrote: From: shazana hilda Subject: [Histonet] IHC scoring system To: histonet@lists.utsouthwestern.edu Date: Saturday, October 25, 2008, 11:35 PM Dear all, I've done the IHC staining step unfortunately encounter problem for a good scoring system (i.e scoring that balance between cell distribution and staining intensity). Hope anyone who have experience in this matter can share they're opinion. Thank you in advance. Cheers, Shazana Hilda Shamsuddin MSc.of Molecular Pathology, Dept. of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: hilda_1075@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Sun Oct 26 09:09:35 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Sun Oct 26 09:10:46 2008 Subject: [Histonet] PAS with Glycogen Digestion - Solution Not Working In-Reply-To: <981878.53101.qm@web65702.mail.ac4.yahoo.com> References: <816E3C72F855F14985FC31D7C963AE6F0AE51629@indexch03.ent.covance.com> <981878.53101.qm@web65702.mail.ac4.yahoo.com> Message-ID: Diastase powder will dissolve in any water, but requires some calcium ions to function. Thus it will not perform in distilled water, but will perform well using plain tap water. Also, the diastase powder from sigma will work well if only a very small amount of the powder is used. We always used just enough to barely cover the bottom of a glass coplin jar and then fill with tap water. I would expect 0.5 grams in 50 mls of water is probably too much. Try using one tenth that amount or 0.05 gm in 50 ml. When there is too much diastase in solution, enzyme conformation is inhibited and it does not work. Spokane water is quite hard. It always worked well at room temperature for 20 minutes. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 24, 2008 8:38 AM To: histonet@lists.utsouthwestern.edu; McKnight, Tanisha Subject: Re: [Histonet] PAS with Glycogen Digestion - Solution Not Working Tanisha: Being an enzyme, you cannot use diastase in distilled water. You have to prepare a diastase buffer at pH7 to dissolve the diastase and do the digestion at 37?C during 30 minutes. That will work. Please, do not try to use saliva! Ren? J. --- On Fri, 10/24/08, McKnight, Tanisha wrote: From: McKnight, Tanisha Subject: [Histonet] PAS with Glycogen Digestion - Solution Not Working To: histonet@lists.utsouthwestern.edu Date: Friday, October 24, 2008, 11:26 AM Hi All: I am in the process of working up a PAS Staining protocol. The PAS stain is working fine, but I am unable to get my tissue to digest. I am using .5g of Diastase in 50ml of DI water. I tried heating the solution in a water bath for 30 min, 1 hour and 1.5 hours and it still looks the same as the one without digestion. Could I be using the wrong Diastase? If so, does anyone have any suggestions for a new product? Thanks, Tanisha Neely, HT(ASCP) AP-Histology/Specimen Management ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.8.3/1747 - Release Date: 10/26/2008 9:27 AM From pruegg <@t> ihctech.net Sun Oct 26 10:03:57 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Oct 26 10:03:56 2008 Subject: [Histonet] Human anti Human In-Reply-To: <582736990810250918n3fedac78rf9b08f2fba23256@mail.gmail.com> Message-ID: The only problem I see with this is similar to running an anti mouse ab on mouse tissue. The secondary link anti human IgG will bind to all IgG in the human tissue, not just the primary antibody, so you would need to use serum blocks to prevent that. I would use 10% serum from the host of the secondary before the primary and then again before the secondary as a block. I would also think about using some human serum in the diluents but you have to be careful not to block all human IgG. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Saturday, October 25, 2008 10:19 AM To: johnsobr@zgi.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Human anti Human Hi, I should start by saying: I have not done this, but this is the way I would approach this problem if I were to encounter it. First I assume you have an human antibody raised in humans right? So, you need an antibody that will detect the human antibody like "Anti-Human IgG (?-chain specific) antibody produced in rabbit" or "Monoclonal Anti-Human IgG (?-chain specific) antibody produced in mouse". (As seen here: http://www.sigmaaldrich.com/life-science/cell-biology/antibodies/antibody-pr oducts.htm?TablePage=14574992. Then you can detect the Rabbit or Mouse secondary with your usual anti Rabbit or Mouse detection. (I would use a polymer just to minimize biotin background.) If anyone sees any holes in that line of approach, I'd love to hear other ideas. It's an interesting problem. Best of luck, Amos Brooks Message: 22 Date: Fri, 24 Oct 2008 09:57:24 -0700 From: "BJON (Brian Johnson)" Subject: [Histonet] Human anti Human To: histonet@lists.utsouthwestern.edu Message-ID: <746E68D5CBB205409B12EC32A61EE4010A2FE8E7@ned.zgi.com> Content-Type: text/plain; charset=us-ascii Hello I am wondering if anyone has found, and or used, an IHC polymer kit for a human anti human monoclonal antibody. Beyond the polymer kit, does anyone have any tips for using a human anti human antibody for IHC without biotinylating it? Thank you. Brian Johnson From anh2006 <@t> med.cornell.edu Sun Oct 26 10:58:58 2008 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Sun Oct 26 11:04:32 2008 Subject: [Histonet] Human anti Human In-Reply-To: <0K9C003YIPXMSD30@mail-gw1.med.cornell.edu> References: <582736990810250918n3fedac78rf9b08f2fba23256@mail.gmail.com><0K9C003YIPXMSD30@mail-gw1.med.cornell.edu> Message-ID: <726943843-1225037064-cardhu_decombobulator_blackberry.rim.net-1390019112-@bxe113.bisx.prod.on.blackberry> Very true Patsy, Amos' proposed scheme will not eliminate the problem of human-on-human. However, I do not see how using extra host secondary serum will block the anti-human from binding the endogenous IgG in the tissue. And using human serum will just make your background worse or eliminate staining altogether by sopping up the anti-human antibody. Speaking from experience doing human anti-human many times, you have a several options some of which are listed below: 1. Preadsorb (in solution in eppendorf) your primary to your bitoinylated secondary (hopefully one of Jackson IR's highly cross adsorbed Abs, maybe even Fc specific or an Fab fragment rather than whole IgG) for a few hours/overnight on a rocker. (Concentrations ratios of both primary and secondary need to be tested on positive control tissue). Then add excess human gamma globulin the tube to sop up any extra unbound anti-human IgG (in fact using a Fab would even be better but more costly than GG). Then add to regularly blocked tissue and detect with streptavidin. If you want more abundant amplification you can use a FITC labeled secondary then detect with rabbit anti-FITC followed by polymer. 2. Label the human anti-human antibody with FITC (very easy to do!). Then detect with rabbit anti-FITC and use rabbit polymer. 3. Biotinylate your primary then use strep to detect. I am curious as to why are you anti-biotinylation? It is your easiest solution to the problem and is not difficult! All of the above techniques work with the right reagents in place. Let me know if you want more information. Good luck, Andrea -----Original Message----- From: Patsy Ruegg Date: Sun, 26 Oct 2008 09:03:57 To: 'Amos Brooks'; ; Subject: RE: [Histonet] Human anti Human The only problem I see with this is similar to running an anti mouse ab on mouse tissue. The secondary link anti human IgG will bind to all IgG in the human tissue, not just the primary antibody, so you would need to use serum blocks to prevent that. I would use 10% serum from the host of the secondary before the primary and then again before the secondary as a block. I would also think about using some human serum in the diluents but you have to be careful not to block all human IgG. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Saturday, October 25, 2008 10:19 AM To: johnsobr@zgi.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Human anti Human Hi, I should start by saying: I have not done this, but this is the way I would approach this problem if I were to encounter it. First I assume you have an human antibody raised in humans right? So, you need an antibody that will detect the human antibody like "Anti-Human IgG (?-chain specific) antibody produced in rabbit" or "Monoclonal Anti-Human IgG (?-chain specific) antibody produced in mouse". (As seen here: http://www.sigmaaldrich.com/life-science/cell-biology/antibodies/antibody-pr oducts.htm?TablePage=14574992. Then you can detect the Rabbit or Mouse secondary with your usual anti Rabbit or Mouse detection. (I would use a polymer just to minimize biotin background.) If anyone sees any holes in that line of approach, I'd love to hear other ideas. It's an interesting problem. Best of luck, Amos Brooks Message: 22 Date: Fri, 24 Oct 2008 09:57:24 -0700 From: "BJON (Brian Johnson)" Subject: [Histonet] Human anti Human To: histonet@lists.utsouthwestern.edu Message-ID: <746E68D5CBB205409B12EC32A61EE4010A2FE8E7@ned.zgi.com> Content-Type: text/plain; charset=us-ascii Hello I am wondering if anyone has found, and or used, an IHC polymer kit for a human anti human monoclonal antibody. Beyond the polymer kit, does anyone have any tips for using a human anti human antibody for IHC without biotinylating it? Thank you. Brian Johnson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Oct 26 12:08:24 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Oct 26 12:08:23 2008 Subject: [Histonet] Human anti Human In-Reply-To: <726943843-1225037064-cardhu_decombobulator_blackberry.rim.net-1390019112-@bxe113.bisx.prod.on.blackberry> Message-ID: Andrea, I agree with your approaches to keep tissue IgG from binding to the anti human secondary, I especially like the fitc conjugation of the primary then use rab anti-fitc, then rab labeled polymer. The reason I avoid SAB is because of endogenous biotin issues. I prefer hrp or ap labeled polymers to avidin biotin systems. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: anh2006@med.cornell.edu [mailto:anh2006@med.cornell.edu] Sent: Sunday, October 26, 2008 9:59 AM To: Patsy Ruegg; histonet@lists.utsouthwestern.edu; johnsobr@zgi.com Subject: Re: [Histonet] Human anti Human Very true Patsy, Amos' proposed scheme will not eliminate the problem of human-on-human. However, I do not see how using extra host secondary serum will block the anti-human from binding the endogenous IgG in the tissue. And using human serum will just make your background worse or eliminate staining altogether by sopping up the anti-human antibody. Speaking from experience doing human anti-human many times, you have a several options some of which are listed below: 1. Preadsorb (in solution in eppendorf) your primary to your bitoinylated secondary (hopefully one of Jackson IR's highly cross adsorbed Abs, maybe even Fc specific or an Fab fragment rather than whole IgG) for a few hours/overnight on a rocker. (Concentrations ratios of both primary and secondary need to be tested on positive control tissue). Then add excess human gamma globulin the tube to sop up any extra unbound anti-human IgG (in fact using a Fab would even be better but more costly than GG). Then add to regularly blocked tissue and detect with streptavidin. If you want more abundant amplification you can use a FITC labeled secondary then detect with rabbit anti-FITC followed by polymer. 2. Label the human anti-human antibody with FITC (very easy to do!). Then detect with rabbit anti-FITC and use rabbit polymer. 3. Biotinylate your primary then use strep to detect. I am curious as to why are you anti-biotinylation? It is your easiest solution to the problem and is not difficult! All of the above techniques work with the right reagents in place. Let me know if you want more information. Good luck, Andrea -----Original Message----- From: Patsy Ruegg Date: Sun, 26 Oct 2008 09:03:57 To: 'Amos Brooks'; ; Subject: RE: [Histonet] Human anti Human The only problem I see with this is similar to running an anti mouse ab on mouse tissue. The secondary link anti human IgG will bind to all IgG in the human tissue, not just the primary antibody, so you would need to use serum blocks to prevent that. I would use 10% serum from the host of the secondary before the primary and then again before the secondary as a block. I would also think about using some human serum in the diluents but you have to be careful not to block all human IgG. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Saturday, October 25, 2008 10:19 AM To: johnsobr@zgi.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Human anti Human Hi, I should start by saying: I have not done this, but this is the way I would approach this problem if I were to encounter it. First I assume you have an human antibody raised in humans right? So, you need an antibody that will detect the human antibody like "Anti-Human IgG (?-chain specific) antibody produced in rabbit" or "Monoclonal Anti-Human IgG (?-chain specific) antibody produced in mouse". (As seen here: http://www.sigmaaldrich.com/life-science/cell-biology/antibodies/antibody-pr oducts.htm?TablePage=14574992. Then you can detect the Rabbit or Mouse secondary with your usual anti Rabbit or Mouse detection. (I would use a polymer just to minimize biotin background.) If anyone sees any holes in that line of approach, I'd love to hear other ideas. It's an interesting problem. Best of luck, Amos Brooks Message: 22 Date: Fri, 24 Oct 2008 09:57:24 -0700 From: "BJON (Brian Johnson)" Subject: [Histonet] Human anti Human To: histonet@lists.utsouthwestern.edu Message-ID: <746E68D5CBB205409B12EC32A61EE4010A2FE8E7@ned.zgi.com> Content-Type: text/plain; charset=us-ascii Hello I am wondering if anyone has found, and or used, an IHC polymer kit for a human anti human monoclonal antibody. Beyond the polymer kit, does anyone have any tips for using a human anti human antibody for IHC without biotinylating it? Thank you. Brian Johnson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Oct 26 12:25:01 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Oct 26 12:25:05 2008 Subject: [Histonet] More than 4 years old secondary antibodies In-Reply-To: <450012a20810241302q73d4769cq4293a93d9514ac00@mail.gmail.com> Message-ID: I would think they are alright if they have been kept lyophilized at 4dc, I would certainly try them. Once you reconstitute them, freeze small aliquots for future use unless they are hrp conjugated. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suhyoung Jeong Sent: Friday, October 24, 2008 2:02 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] More than 4 years old secondary antibodies Hello everyone, I got some still-lyophilyzed kept-in-4C secondary antibodies recently. We don't know exactly how old they are, but the company (Zymed) could not track the lot number on the bottle. They said that they discard the record after 4 years and that makes these antibodies at least 4 years old. I assume between 5-7 years old. Has anyone have some idea whether it will be worth testing these antibodies? Thank you in advance Regards, Suh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Oct 26 12:29:24 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sun Oct 26 12:29:20 2008 Subject: [Histonet] Need help for CD95(FAS) and antigen retrieval system In-Reply-To: <291774.83461.qm@web90506.mail.mud.yahoo.com> Message-ID: What detection system are you using? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of soofia siddiqui Sent: Friday, October 24, 2008 12:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Need help for CD95(FAS) and antigen retrieval system Hi everyone, I am sure some one in this large world of histonet will be able to help me. I am trying to do CD95 staining on human skin FF PE tissue sections. CD95 is 45 KD single chain type cell surface??protein that mediates apoptosis when crosslinked with agnostic anti-Fas antibodies.I have tried citric acid pH 6.0 buffer in microwave and and 99 degree Celsius?water bath incubator and also tried sodium citrate Buffer pH 6.00 on microwave and incubator and also?Trypsin for antigen retrieval, But I could not get any success. I am using R&D MAB 142 monoclonal antibody Clone DX2 and also using BioLegend 3056130 monoclonal antibody clone DX2, but none of the method worked to get the signaling. Please provide me,?if?? you ?have some suggestion or any better antigen retrieval method for Fas antibodies. Thank you in advance.? Soofia UWHC,Madison, WI? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Sun Oct 26 13:12:38 2008 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Sun Oct 26 13:18:45 2008 Subject: AW: [Histonet] synonym of Factor VIII? In-Reply-To: <69F96D41F9294BD09507A9517E3B3268@dielangs.at> References: <102520082320.5135.4903A9A7000308B60000140F22230650629B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net><69F96D41F9294BD09507A9517E3B3268@dielangs.at> Message-ID: <1461192956-1225045056-cardhu_decombobulator_blackberry.rim.net-360798474-@bxe113.bisx.prod.on.blackberry> Hi Gudrun, Any antibody I have used for vWF detects the complex (DAKO's A0082 pAb for example) ... I believe the Factor VIIIa comes into play once cleaved as you mention below. Is this something you need to specifically be concerned with? I thought you/your PI just wanted a good pan endothelial antibody? If you want an antibody to detect endothelial cells (EC) this is a good one but be aware vWF is also expressed in megas and gives a punctate expression pattern as it is localized to EC Weibel-Pallade bodies and platelets. I still use it all the time as it is so robust a marker. Have you tried VE-cadherin? It is a good pan marker in IHC for ECs. Good luck, Andrea -----Original Message----- From: Gudrun Lang Date: Sun, 26 Oct 2008 08:55:36 To: Subject: AW: [Histonet] synonym of Factor VIII? Many thanks to all that answered my question, but I am still in doubt. This is from Wikipedia: "Von Willebrand factor is not an enzyme and therefore has no catalytic activity. Its primary function is binding to other proteins, particularly Factor VIII and it is important in platelet adhesion to wound sites". "Factor VIII is bound to vWF while inactive in circulation; Factor VIII degrades rapidly when not bound to vWF. Factor VIII is released from vWF by the action of thrombin." Could it be possible, that in our technical manner the two proteins are seen as one, because Factor VIII alone can hardly be detected? Gudrun Lang _____ Von: renafail@bellsouth.net [mailto:renafail@bellsouth.net] Gesendet: Sonntag, 26. Oktober 2008 00:20 An: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Cc: Gudrun Lang Betreff: Re: [Histonet] synonym of Factor VIII? Factor VIII is also known as vonWillebrand factor. I don't recall a Factor VIIIa.though there may very be one. There is a however a Factor XIIIa not the same at all. Rena -------------- Original message from "Gudrun Lang" : -------------- > Hi all, > > I need some help to find the right antibody for IHC. My pathologists told me > to find a Factor VIIIa - antibody. It should be a good marker for > angioendothels. > > When I search, always the "vonWillebrand Factor" appears. And I don't know, > if that are synonyms for the same antigen. > > And if there even exists an VIII a, with stress on "a". > > > > Can anyone help me? Or even recommend an antibody (Ventana Benchmark XT)? > > Bye > > Gudrun Lang > > > > Histolabor, Akh Linz, Austria > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Sun Oct 26 14:49:56 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Sun Oct 26 14:50:01 2008 Subject: [Histonet] Re: synonym of Factor VIII? Message-ID: Factor VIII (factor 8, anti-hemophilic factor) is present in vascular endothelium and, and along with CD34 is used as an immunohistochemical marker for small blood vessels. Factor VIIIa is the activated form of this clotting factor. von Willebrand factor is an entirely different molecule, also in vascular endothelium, and is bound to circulating factor VIII. There are fairly clear accounts of these factors in Wikipedia. Bob Richmond Samurai Pathologist Knoxville TN From cfarish <@t> csu.edu.au Sun Oct 26 18:19:25 2008 From: cfarish <@t> csu.edu.au (Farish, Craig) Date: Sun Oct 26 18:19:47 2008 Subject: [Histonet] RE (Histonet) Bones Message-ID: <79A000B60AFE8045BA2581119DFEC44404AC544D@ESWW01.CSUMain.csu.edu.au> Hi Ian, Dermestid beetles are definitely the least smelly option (although they are not perfect). You'll find that there will usually be a residual smell in the short term. They can, however, damage small or delicate bones and the best way to deal with these is by bacterial maceration. This smells beyond belief. The anatomy dept here uses flow hoods but you can still smell it from the other side of the building. I've read about people putting them in tubs and leaving them outside(a very long way away) but that's probably not an option in the West End of Glasgow. When they are done, do not use bleach to whiten the bones as that makes them brittle - use dilute hydrogen peroxide (around 3 - 5%). It's worth trying some of the taxidermy forums as well - taxidermy.net is a good place to start. Have fun, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga NSW 2678 Australia ' I don't want to achieve immortality through my work, I want to achieve immortality through not dying' - Woody Allen From jaustin1967 <@t> gmail.com Sun Oct 26 19:14:47 2008 From: jaustin1967 <@t> gmail.com (Michael Bradley) Date: Sun Oct 26 19:14:52 2008 Subject: [Histonet] Automated Immunostainers Message-ID: Hi all My lab is interested in purchasing an automated immunohistochemistry stainer and I would like some feedback on the new stainers that are out there. Is anyone using the Celerus Wave or Biocare's Intellepath stainer. Any opinions on either of these stainers, good and bad is greatly appreciated. I have seen the ventana, leica and the dako stainers and unless they have come out with something new I have all the info I need on those stainers. Thank you in advance. From anhtx <@t> drreddys.com Sun Oct 26 22:05:04 2008 From: anhtx <@t> drreddys.com (anhtx@drreddys.com) Date: Sun Oct 26 21:56:37 2008 Subject: [Histonet] mitotic figures-staining protocol Message-ID: Thanks Carl for your valuable suggestions on anti- phospho-Histone H3 antibody. But i will be glad if someone can share the staining protocol for Toluidine blue and Crystal violet to demonstrate mitotic figures. Regards Girish Disclaimer This message contains legally privileged and/or confidential information. If you are not the intended recipient(s), or employee or agent responsible for delivery of this message to the intended recipient(s), you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this message in error, please immediately notify the sender and delete this e-mail message from your computer. WARNING: Computer viruses can be transmitted via email. The recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email. From girishbc <@t> drreddys.com Sun Oct 26 22:38:06 2008 From: girishbc <@t> drreddys.com (girishbc@drreddys.com) Date: Sun Oct 26 22:29:40 2008 Subject: Fw: [Histonet] Protect fingers during Microtomy Message-ID: Dear Tahseen 1) You can contact your microtome vendor for blade cover/shutter 2) Be careful while sectioning, offcourse this come by experience. 3)Using glove will invite more trouble and may not be handy. Regards Chandrashekar BG ----- Forwarded by BC Girish/PreClinSafety/DR-India/DRL/IN on 27/10/2008 09:03 AM ----- tahseen@brain.net .pk Sent by: To histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] Protect fingers during 25/10/2008 04:34 Microtomy PM Hi Histoneters, I am looking some think (like gloves)to protect the fingers during Microtomy. Thanks, Muhammad Tahseen Histology supervisor SKMCH&RC Lahore,Pakistan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer This message contains legally privileged and/or confidential information. If you are not the intended recipient(s), or employee or agent responsible for delivery of this message to the intended recipient(s), you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this message in error, please immediately notify the sender and delete this e-mail message from your computer. WARNING: Computer viruses can be transmitted via email. The recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email. From AWeiss <@t> shorememorial.org Mon Oct 27 07:01:14 2008 From: AWeiss <@t> shorememorial.org (AWeiss@shorememorial.org) Date: Mon Oct 27 07:02:14 2008 Subject: [Histonet] Re: Histonet Digest, Vol 59, Issue 32 In-Reply-To: <20081025170100.120C268BFD9@smhex1.shorememorial.org> Message-ID: I am a cytotechnologist with approx. the same amount of workload. My workload has turned around to be mostly Non gyn FNA of thyroid primarily. Management expects me to manage a flow department and keep it compliant to CAP, also to keep cytology compliant with CAP and do all the prepping and screening. It will not work, that 0.5FTE preps I assume and frees up the technologist to do all the work that is required, screening cases within so many hours, and doing all the QC/QC which is alot of back work required by CAP. So your tech is correct, they will fall behind. Even though the volume of cases appear low there are numerous slides associated with each case especially FNA's. I can screen approx. 75 slds, if I have someone prepping but without anyone I would only probably do 35-40 per day. Do not forget the cytotechnologist also needs time for their proficiency work and continuing education in whatever form it may have. I have been a tech for 24 years and have worked in both a private lab setting and also community hospital settings, so yes there has been a change but the work still takes time for quality work to be accomplished. We have one full time Cytology Tech Specialist and 0.5 lab assistant for cyto prep. We are a low volume non-gyn lab (2500 cases YTD) - totally ThinPrep except for thyroid aspirates that come from one practice with up to 5 extra smeared slides. Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org From Katie.Sousa <@t> bhs.org Mon Oct 27 09:19:10 2008 From: Katie.Sousa <@t> bhs.org (Sousa, Katie) Date: Mon Oct 27 09:19:44 2008 Subject: [Histonet] Uranyl nitrate Message-ID: <3115ECE23B13C94CACDB17711212E7D868D1CB63EA@bhsexc01.bhs.org> Does anyone use a chemical other than Uranyl nitrate when performing a Steiner/Modified Steiner stain? I've been asked by my safety dept to discontinue use of this chemical as it is expensive to hazwaste it. ----------------------------------------- CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at (413) 794-0000 and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet web site at http://www.baystatehealth.com. From leiker <@t> buffalo.edu Mon Oct 27 09:27:30 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Mon Oct 27 09:27:39 2008 Subject: [Histonet] Re: synonym of Factor VIII? In-Reply-To: References: Message-ID: <4A91AD3DC86465C5817911DD@bchwxp2702.ad.med.buffalo.edu> Bob - I'm curious as to what you as a "Samurai Pathologist" are and do. Do you use swords to cut paraffin blocks? ;-) --On Sunday, October 26, 2008 3:49 PM -0400 Robert Richmond wrote: > Factor VIII (factor 8, anti-hemophilic factor) is present in vascular > endothelium and, and along with CD34 is used as an immunohistochemical > marker for small blood vessels. Factor VIIIa is the activated form of > this clotting factor. > > von Willebrand factor is an entirely different molecule, also in > vascular endothelium, and is bound to circulating factor VIII. > > There are fairly clear accounts of these factors in Wikipedia. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (packages) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 "Without my flaws I'm really very boring." - random internet blog commentator From leiker <@t> buffalo.edu Mon Oct 27 09:28:32 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Mon Oct 27 09:28:42 2008 Subject: [Histonet] RE (Histonet) Bones In-Reply-To: <79A000B60AFE8045BA2581119DFEC44404AC544D@ESWW01.CSUMain.csu.edu.au> References: <79A000B60AFE8045BA2581119DFEC44404AC544D@ESWW01.CSUMain.csu.edu .au> Message-ID: This cleaning-bones discussion keeps getting better and better! What an excellent topic for this Halloween week! --On Monday, October 27, 2008 10:19 AM +1100 "Farish, Craig" wrote: > Hi Ian, > > Dermestid beetles are definitely the least smelly option > (although they are not perfect). You'll find that there will usually be > a residual smell in the short term. They can, however, damage small or > delicate bones and the best way to deal with these is by bacterial > maceration. This smells beyond belief. The anatomy dept here uses flow > hoods but you can still smell it from the other side of the building. > I've read about people putting them in tubs and leaving them outside(a > very long way away) but that's probably not an option in the West End of > Glasgow. When they are done, do not use bleach to whiten the bones as > that makes them brittle - use dilute hydrogen peroxide (around 3 - 5%). > It's worth trying some of the taxidermy forums as well - taxidermy.net > is a good place to start. > > Have fun, Craig > > > > Craig (Joe) Farish > > Senior Technical Officer > > Veterinary Diagnostics > > School of Animal and Veterinary Sciences > > Charles Sturt University > > Wagga Wagga NSW 2678 > > Australia > > > > ' I don't want to achieve immortality through my work, I want to achieve > immortality through not dying' - Woody Allen > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (packages) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 "Without my flaws I'm really very boring." - random internet blog commentator From JWeems <@t> sjha.org Mon Oct 27 10:17:51 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Oct 27 10:18:28 2008 Subject: [Histonet] Textiles/Fibers Message-ID: <5D64396A0D4A5346BEBC759022AAEAA50E3F15@ITSSSXM01V6.one.ads.che.org> Happy Monday Everyone! Do we have anyone who work with textiles and cut thread and fibers, etc.? If so, would you please contact me off line? Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From KKay <@t> chr.ab.ca Mon Oct 27 10:36:16 2008 From: KKay <@t> chr.ab.ca (Kay, Karen) Date: Mon Oct 27 10:36:25 2008 Subject: [Histonet] RE: Histonet Digest, Vol 59, Issue 32 RE PAS DIGESTION In-Reply-To: Message-ID: <9C0BD812BAB0BA4DB7E7FD4F5FA3CAA80A494D0D@exbe.chr.ab.ca> Good Day Tanisha, We use the following diastase solution and timing protocol. It is consistent and works very well. 1. Diastase Solution Amylase (Type VI-B from Porcine Pancreas - Sigma A3176) 0.5 g (Stored in fridge at 4?C) Tap water 50 mL Put 50 mL of warm water from the tap (37C) into a coplin jar (check temperature of water with thermometer). Add 0.5 g of amylase and stir well. Use once and discard. Prewarm this solution in a 37 C degree oven prior to placing slides into it for 1 hour. Hope this will help you. Karen J Kay, MLT Pathology Supervisor Chinook Health Laboratory Chinook Regional Hospital 960 - 19 st South Lethbridge, Alberta, Canada T1J 1W5 (403) 388 - 6061 (Phone) (403) 388 - 6067 (Fax) > Hi All: > > I am in the process of working up a PAS Staining protocol. The PAS > stain is working fine, but I am unable to get my tissue to digest. I > am using .5g of Diastase in 50ml of DI water. I tried heating the > solution in a water bath for 30 min, 1 hour and 1.5 hours and it still > looks the same as the one without digestion. Could I be using the > wrong Diastase? If so, does anyone have any suggestions for a new > product? > > Thanks, > > Tanisha Neely, HT(ASCP) > AP-Histology/Specimen Management This communication is intended for the use of the recipient to which it is addressed, and may contain confidential, personal and or privileged information. Please contact us immediately if you are not the intended recipient. Do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. From rjbuesa <@t> yahoo.com Mon Oct 27 12:04:00 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 27 12:04:06 2008 Subject: [Histonet] Uranyl nitrate In-Reply-To: <3115ECE23B13C94CACDB17711212E7D868D1CB63EA@bhsexc01.bhs.org> Message-ID: <299110.19894.qm@web65711.mail.ac4.yahoo.com> Katie: I developed a method that uses phosphotungstic acid. Under separate cover I am sending you the article. Ren? J. --- On Mon, 10/27/08, Sousa, Katie wrote: From: Sousa, Katie Subject: [Histonet] Uranyl nitrate To: "'histonet@lists.utsouthwestern.edu'" Date: Monday, October 27, 2008, 10:19 AM Does anyone use a chemical other than Uranyl nitrate when performing a Steiner/Modified Steiner stain? I've been asked by my safety dept to discontinue use of this chemical as it is expensive to hazwaste it. ----------------------------------------- CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at (413) 794-0000 and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet web site at http://www.baystatehealth.com. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cheastys <@t> svm.vetmed.wisc.edu Mon Oct 27 12:15:51 2008 From: cheastys <@t> svm.vetmed.wisc.edu (Sandra Cheasty) Date: Mon Oct 27 12:16:06 2008 Subject: [Histonet] CD20 suddenly has high background Message-ID: We do CD20 on canine and feline samples and are suddenly getting lots of non-specific background. We use: CD3, F7.2.38 from DAKO diluted 1:400 LSAB+ and DAB+ Avidin and Biotin block I even tried it with a polymer detection kit, but to no avail. Has anyone else had recent difficulty with this T cell marker? Thanks, Sandy From lab.mef <@t> smmc.org Mon Oct 27 13:33:02 2008 From: lab.mef <@t> smmc.org (Meredith Fuller-Fedorczyk) Date: Mon Oct 27 13:33:20 2008 Subject: [Histonet] Protect fingers during Microtomy References: <55411.202.125.145.178.1224932695.squirrel@brain.net.pk> Message-ID: <00ba01c93862$721f3c90$240c0180@PCLAB11> Hi, Muhammad Tahseen Surgipath Company makes a cut resistant glove in small, medium or large. The gloves are reusable. We only use them when we clean out the cryostat between frozen sections. This is to get behind the knife inside the cryostat where all the shavings fall. We do not use them for regular cutting with a microtome. It is not necessary. Meredith ----- Original Message ----- From: To: Sent: Saturday, October 25, 2008 6:04 AM Subject: [Histonet] Protect fingers during Microtomy Hi Histoneters, I am looking some think (like gloves)to protect the fingers during Microtomy. Thanks, Muhammad Tahseen Histology supervisor SKMCH&RC Lahore,Pakistan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Mon Oct 27 15:17:10 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Oct 27 15:18:52 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <00ba01c93862$721f3c90$240c0180@PCLAB11> References: <55411.202.125.145.178.1224932695.squirrel@brain.net.pk> <00ba01c93862$721f3c90$240c0180@PCLAB11> Message-ID: <490621C6.60304@umdnj.edu> > I am looking some think (like gloves)to protect the fingers during microtomy No offense, but if you're that prone to injury, I recommend steering clear of microtomes in general ;) From Jackie.O'Connor <@t> abbott.com Mon Oct 27 15:43:30 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Oct 27 15:43:59 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <490621C6.60304@umdnj.edu> Message-ID: OK - time to poll - who has had sutures from microtomy? Me! 23 years ago - 8 months pregnant, and not paying attention to what I was doing. Oh yeah, and one other time, I cut off the tip of my finger beta testing a microtome safety invention from EHS. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 10/27/2008 03:17 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Protect fingers during Microtomy > I am looking some think (like gloves)to protect the fingers during microtomy No offense, but if you're that prone to injury, I recommend steering clear of microtomes in general ;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Mon Oct 27 15:50:14 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Oct 27 15:50:18 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: References: Message-ID: <49062986.7080800@pathology.washington.edu> Yes, nicked the corner of a steel blade reaching for slide. This was back in 78 or 79. It was a Saturday, working by myself and I had to wait for 3 hours in the ER. No preferential treatment for employees in a county hospital. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Jackie M O'Connor wrote: > OK - time to poll - who has had sutures from microtomy? Me! 23 years > ago - 8 months pregnant, and not paying attention to what I was doing. Oh > yeah, and one other time, I cut off the tip of my finger beta testing a > microtome safety invention from EHS. > > > > > > Peter Carroll > Sent by: histonet-bounces@lists.utsouthwestern.edu > 10/27/2008 03:17 PM > > To > > cc > histonet@lists.utsouthwestern.edu > Subject > Re: [Histonet] Protect fingers during Microtomy > > > > > > > > I am looking some think (like gloves)to protect the fingers during > microtomy > > No offense, but if you're that prone to injury, I recommend steering > clear of microtomes in general ;) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From STapper <@t> smdc.org Mon Oct 27 16:03:07 2008 From: STapper <@t> smdc.org (Tapper, Sheila J.) Date: Mon Oct 27 16:03:16 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <49062986.7080800@pathology.washington.edu> References: <49062986.7080800@pathology.washington.edu> Message-ID: We keep a "tip" jar. So far we have had 2 donations! It is a good deterrent if nothing else! Sheila Tapper HT(ASCP) Anatomic Pathology Supervisor SMDC Clinical Laboratory 407 East First Street Duluth, MN 55804 Telephone: 218-786-5472 Fax: 218-786-2369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Monday, October 27, 2008 3:50 PM To: Jackie M O'Connor Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Protect fingers during Microtomy Yes, nicked the corner of a steel blade reaching for slide. This was back in 78 or 79. It was a Saturday, working by myself and I had to wait for 3 hours in the ER. No preferential treatment for employees in a county hospital. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Jackie M O'Connor wrote: > OK - time to poll - who has had sutures from microtomy? Me! 23 years > ago - 8 months pregnant, and not paying attention to what I was doing. Oh > yeah, and one other time, I cut off the tip of my finger beta testing a > microtome safety invention from EHS. > > > > > > Peter Carroll > Sent by: histonet-bounces@lists.utsouthwestern.edu > 10/27/2008 03:17 PM > > To > > cc > histonet@lists.utsouthwestern.edu > Subject > Re: [Histonet] Protect fingers during Microtomy > > > > > > > > I am looking some think (like gloves)to protect the fingers during > microtomy > > No offense, but if you're that prone to injury, I recommend steering > clear of microtomes in general ;) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From JWeems <@t> sjha.org Mon Oct 27 16:04:14 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Oct 27 16:04:19 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: References: <490621C6.60304@umdnj.edu> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA50E402B@ITSSSXM01V6.one.ads.che.org> That would be me... Real blade - cleaning it. Those were the days...too long ago to say! (And we won't count the nicks and scrapes that probably should have been.) j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, October 27, 2008 4:44 PM To: Peter Carroll Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Protect fingers during Microtomy OK - time to poll - who has had sutures from microtomy? Me! 23 years ago - 8 months pregnant, and not paying attention to what I was doing. Oh yeah, and one other time, I cut off the tip of my finger beta testing a microtome safety invention from EHS. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 10/27/2008 03:17 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Protect fingers during Microtomy > I am looking some think (like gloves)to protect the fingers during microtomy No offense, but if you're that prone to injury, I recommend steering clear of microtomes in general ;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From mpence <@t> grhs.net Mon Oct 27 16:07:25 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Oct 27 16:07:31 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A395C@IS-E2K3.grhs.net> I have cut the tips off both my index fingers. They are now flat on the medial sides. Have sliced various fingers from time to time hitting the "edge" of the blade. Goes with the job. Most of the techs that work for me have had one or two cuts over the years. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tapper, Sheila J. Sent: Monday, October 27, 2008 4:03 PM To: Victor Tobias; Jackie M O'Connor Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy We keep a "tip" jar. So far we have had 2 donations! It is a good deterrent if nothing else! Sheila Tapper HT(ASCP) Anatomic Pathology Supervisor SMDC Clinical Laboratory 407 East First Street Duluth, MN 55804 Telephone: 218-786-5472 Fax: 218-786-2369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Monday, October 27, 2008 3:50 PM To: Jackie M O'Connor Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Protect fingers during Microtomy Yes, nicked the corner of a steel blade reaching for slide. This was back in 78 or 79. It was a Saturday, working by myself and I had to wait for 3 hours in the ER. No preferential treatment for employees in a county hospital. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Jackie M O'Connor wrote: > OK - time to poll - who has had sutures from microtomy? Me! 23 years > ago - 8 months pregnant, and not paying attention to what I was doing. Oh > yeah, and one other time, I cut off the tip of my finger beta testing a > microtome safety invention from EHS. > > > > > > Peter Carroll > Sent by: histonet-bounces@lists.utsouthwestern.edu > 10/27/2008 03:17 PM > > To > > cc > histonet@lists.utsouthwestern.edu > Subject > Re: [Histonet] Protect fingers during Microtomy > > > > > > > > I am looking some think (like gloves)to protect the fingers during > microtomy > > No offense, but if you're that prone to injury, I recommend steering > clear of microtomes in general ;) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Mon Oct 27 16:20:49 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Mon Oct 27 16:20:58 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: References: <49062986.7080800@pathology.washington.edu> Message-ID: <8B8F2E4C4395D63780C79AB9@bchwxp2702.ad.med.buffalo.edu> (These Histonet Halloween topics just keep getting better and better...) I've cut my thumb on a cryostat blade a couple months ago - forgot to lock the wheel while adjusting the specimen in the holder. Thank God it left an intact (though rather large) flap of skin on one side, so no need for sutures, just kept it compressed for oh several days. Yeah I've cut myself on microtome blades prior to that as well, though not as badly. But then, I tend to be an absent-minded clutz at times! Merced --On Monday, October 27, 2008 4:03 PM -0500 "Tapper, Sheila J." wrote: > We keep a "tip" jar. So far we have had 2 donations! It is a good > deterrent if nothing else! > > Sheila Tapper HT(ASCP) > Anatomic Pathology Supervisor > > SMDC Clinical Laboratory > 407 East First Street > Duluth, MN 55804 > > Telephone: 218-786-5472 > Fax: 218-786-2369 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor > Tobias > Sent: Monday, October 27, 2008 3:50 PM > To: Jackie M O'Connor > Cc: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Protect fingers during Microtomy > > Yes, nicked the corner of a steel blade reaching for slide. This was > back in 78 or 79. It was a Saturday, working by myself and I had to wait > > for 3 hours in the ER. No preferential treatment for employees in a > county hospital. > > Victor > > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > > Jackie M O'Connor wrote: >> OK - time to poll - who has had sutures from microtomy? Me! 23 > years >> ago - 8 months pregnant, and not paying attention to what I was doing. > Oh >> yeah, and one other time, I cut off the tip of my finger beta testing > a >> microtome safety invention from EHS. >> >> >> >> >> >> Peter Carroll >> Sent by: histonet-bounces@lists.utsouthwestern.edu >> 10/27/2008 03:17 PM >> >> To >> >> cc >> histonet@lists.utsouthwestern.edu >> Subject >> Re: [Histonet] Protect fingers during Microtomy >> >> >> >> >> >> >> > I am looking some think (like gloves)to protect the fingers during >> microtomy >> >> No offense, but if you're that prone to injury, I recommend steering >> clear of microtomes in general ;) >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This e-mail communication and any attachments may contain confidential > and privileged information for the use of the designated recipients named > above. If you are not the intended recipient, you are hereby notified > that you have received this communication in error and that any review, > disclosure, dissemination, distribution or copying of it or its contents > is prohibited. As required by federal and state laws, you need to hold > this information as privileged and confidential. If you have received > this communication in error, please notify the sender and destroy all > copies of this communication and any attachments. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (pkgs) / 140 Farber Hall (letters) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 "Without my flaws I'm really very boring." - random internet blog commentator From CIngles <@t> uwhealth.org Mon Oct 27 16:24:11 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Mon Oct 27 16:24:16 2008 Subject: [Histonet] Protect fingers during Microtomy References: <661949901A768E4F9CC16D8AF8F2838C017A395C@IS-E2K3.grhs.net> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120191@uwhis-xchng3.uwhis.hosp.wisc.edu> I haven't lost any fingertips...yet...(knock on formica table top). Have had a couple fingernail trims. But I have only been a histo for 7 years. Plenty of time yet though. Spoiled with disposable blades and employee safety officers I guess. Mohs work keeps me more on my toes I think. I haven't nicked myself in the 3 years I have been working Mohs. I did manage to work with an automated microtome (foot petal operated) for over a year without losing anything. Sure gave me lightning fast reflexes though. Those things are dangerous in my book! Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mike Pence Sent: Mon 10/27/2008 4:07 PM To: Tapper, Sheila J.; Victor Tobias; Jackie M O'Connor Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy I have cut the tips off both my index fingers. They are now flat on the medial sides. Have sliced various fingers from time to time hitting the "edge" of the blade. Goes with the job. Most of the techs that work for me have had one or two cuts over the years. Mike From jhnspam <@t> aol.com Mon Oct 27 16:39:17 2008 From: jhnspam <@t> aol.com (pam johnson) Date: Mon Oct 27 16:39:29 2008 Subject: [Histonet] Histology and immuonhistochemistry of Drosophila eye sections Message-ID: <8CB06939959662F-D00-13E7@webmail-md01.sysops.aol.com> I have been approached by an investigator?who wants us to do some work on fly eyes. Has anyone had any experience with fly eyes in paraffin? If so can you please share your protocols on processing and cutting. Aren't they very crunchy? Do you process the whole head? ? Any information would be greatly appreciated. Thanks, Pam Johnson, BS, HT (ASCP) Lab Manager Veterinary Pathology Core Lab St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN? 38105-3678 Office - 901-595-3355 From CIngles <@t> uwhealth.org Mon Oct 27 20:46:44 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Mon Oct 27 20:48:50 2008 Subject: [Histonet] Histology and immuonhistochemistry of Drosophila eyesections (OT) References: <8CB06939959662F-D00-13E7@webmail-md01.sysops.aol.com> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120192@uwhis-xchng3.uwhis.hosp.wisc.edu> Fly's eyes? What about bee's knees? Sorry it's almost 9pm. I'm fizzling out. Claire :o ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of pam johnson Sent: Mon 10/27/2008 4:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology and immuonhistochemistry of Drosophila eyesections I have been approached by an investigator?who wants us to do some work on fly eyes. Has anyone had any experience with fly eyes in paraffin? If so can you please share your protocols on processing and cutting. Aren't they very crunchy? Do you process the whole head? ? Any information would be greatly appreciated. Thanks, Pam Johnson, BS, HT (ASCP) Lab Manager Veterinary Pathology Core Lab St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN? 38105-3678 Office - 901-595-3355 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hilda_1075 <@t> yahoo.com Tue Oct 28 02:34:33 2008 From: hilda_1075 <@t> yahoo.com (shazana hilda) Date: Tue Oct 28 02:34:39 2008 Subject: [Histonet] IHC scoring system Message-ID: <695781.34010.qm@web38805.mail.mud.yahoo.com> Thank so much for all response. It's helps a lot. Shazana Hilda Shamsuddin MSc.of Molecular Pathology, Dept. of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: hilda_1075@yahoo.com From tpodawiltz <@t> lrgh.org Tue Oct 28 04:19:46 2008 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Oct 28 04:19:56 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <8B8F2E4C4395D63780C79AB9@bchwxp2702.ad.med.buffalo.edu> References: <49062986.7080800@pathology.washington.edu> , <8B8F2E4C4395D63780C79AB9@bchwxp2702.ad.med.buffalo.edu> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D2D14DB2F@LRGHEXVS1.practice.lrgh.org> Knock on wood, I have not cut myself in several years. However, over the years I have had several staff members who have done it. When I was in the Navy back in 81, one guy took off a finger tip at the first joint. I had a new Tech right out of school that cut herself pretty good, took off one side of finger tip, fortunately not deep. My only comment to her was to tell her that now she was an official tech, then had another tech take her to the ER. What I find funny is I have one Pathologist that complains about us locking the handle on the cryostat when we clean it. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced Leiker [leiker@buffalo.edu] Sent: Monday, October 27, 2008 5:20 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy (These Histonet Halloween topics just keep getting better and better...) I've cut my thumb on a cryostat blade a couple months ago - forgot to lock the wheel while adjusting the specimen in the holder. Thank God it left an intact (though rather large) flap of skin on one side, so no need for sutures, just kept it compressed for oh several days. Yeah I've cut myself on microtome blades prior to that as well, though not as badly. But then, I tend to be an absent-minded clutz at times! Merced --On Monday, October 27, 2008 4:03 PM -0500 "Tapper, Sheila J." wrote: > We keep a "tip" jar. So far we have had 2 donations! It is a good > deterrent if nothing else! > > Sheila Tapper HT(ASCP) > Anatomic Pathology Supervisor > > SMDC Clinical Laboratory > 407 East First Street > Duluth, MN 55804 > > Telephone: 218-786-5472 > Fax: 218-786-2369 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor > Tobias > Sent: Monday, October 27, 2008 3:50 PM > To: Jackie M O'Connor > Cc: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Protect fingers during Microtomy > > Yes, nicked the corner of a steel blade reaching for slide. This was > back in 78 or 79. It was a Saturday, working by myself and I had to wait > > for 3 hours in the ER. No preferential treatment for employees in a > county hospital. > > Victor > > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > > Jackie M O'Connor wrote: >> OK - time to poll - who has had sutures from microtomy? Me! 23 > years >> ago - 8 months pregnant, and not paying attention to what I was doing. > Oh >> yeah, and one other time, I cut off the tip of my finger beta testing > a >> microtome safety invention from EHS. >> >> >> >> >> >> Peter Carroll >> Sent by: histonet-bounces@lists.utsouthwestern.edu >> 10/27/2008 03:17 PM >> >> To >> >> cc >> histonet@lists.utsouthwestern.edu >> Subject >> Re: [Histonet] Protect fingers during Microtomy >> >> >> >> >> >> >> > I am looking some think (like gloves)to protect the fingers during >> microtomy >> >> No offense, but if you're that prone to injury, I recommend steering >> clear of microtomes in general ;) >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This e-mail communication and any attachments may contain confidential > and privileged information for the use of the designated recipients named > above. If you are not the intended recipient, you are hereby notified > that you have received this communication in error and that any review, > disclosure, dissemination, distribution or copying of it or its contents > is prohibited. As required by federal and state laws, you need to hold > this information as privileged and confidential. If you have received > this communication in error, please notify the sender and destroy all > copies of this communication and any attachments. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (pkgs) / 140 Farber Hall (letters) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 "Without my flaws I'm really very boring." - random internet blog commentator _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From abright <@t> brightinstruments.com Tue Oct 28 06:58:55 2008 From: abright <@t> brightinstruments.com (Alan Bright) Date: Tue Oct 28 07:32:09 2008 Subject: [Histonet] Textiles/Fibers In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA50E3F15@ITSSSXM01V6.one.ads.che.org> References: <5D64396A0D4A5346BEBC759022AAEAA50E3F15@ITSSSXM01V6.one.ads.che.org> Message-ID: <3EFBB875DEE1994FB040A0B099F3AC8A0ACD01@BRIGHT-SBS.Bright.local> Dear Joyce, I should be able to assist as I have experience with these samples sectioned in a cryostat. You can contact me on Skype in your afternoon my evening if that's a help. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: 27 October 2008 15:18 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Textiles/Fibers Happy Monday Everyone! Do we have anyone who work with textiles and cut thread and fibers, etc.? If so, would you please contact me off line? Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mlm11 <@t> cornell.edu Tue Oct 28 07:40:12 2008 From: mlm11 <@t> cornell.edu (Mary Lou Norman) Date: Tue Oct 28 07:40:20 2008 Subject: [Histonet] sectioning fees Message-ID: <6.2.1.2.2.20081028083508.01e289f0@postoffice9.mail.cornell.edu> Hello, I would like to know what people charge for sectioning all the way through a paraffin block keeping all sections in tie boxes. Intermittent sections will be taken for H&E. Should I charge an hourly rate just for the sectioning? I will charge for the individual slides made. Thanks, Mary Lou From HornHV <@t> archildrens.org Tue Oct 28 07:41:00 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Oct 28 07:41:15 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <49062986.7080800@pathology.washington.edu> References: <49062986.7080800@pathology.washington.edu> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82EAF@EMAIL.archildrens.org> I've had stitches twice. Once 34 years ago, I rolled my thumb between the block and the blade. (from rolling my thumb over the block to warm it a bit as I was turning the wheel of the microtome.....didn't do that anymore!!) And once 21 years ago when I was 9 months pregnant I slashed my little finger on the end of the blade... Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Monday, October 27, 2008 3:50 PM To: Jackie M O'Connor Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Protect fingers during Microtomy Yes, nicked the corner of a steel blade reaching for slide. This was back in 78 or 79. It was a Saturday, working by myself and I had to wait for 3 hours in the ER. No preferential treatment for employees in a county hospital. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Jackie M O'Connor wrote: > OK - time to poll - who has had sutures from microtomy? Me! 23 years > ago - 8 months pregnant, and not paying attention to what I was doing. Oh > yeah, and one other time, I cut off the tip of my finger beta testing a > microtome safety invention from EHS. > > > > > > Peter Carroll > Sent by: histonet-bounces@lists.utsouthwestern.edu > 10/27/2008 03:17 PM > > To > > cc > histonet@lists.utsouthwestern.edu > Subject > Re: [Histonet] Protect fingers during Microtomy > > > > > > > > I am looking some think (like gloves)to protect the fingers during > microtomy > > No offense, but if you're that prone to injury, I recommend steering > clear of microtomes in general ;) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From annigyg <@t> gmail.com Tue Oct 28 08:06:12 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Tue Oct 28 08:06:17 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82EAF@EMAIL.archildrens.org> References: <49062986.7080800@pathology.washington.edu> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82EAF@EMAIL.archildrens.org> Message-ID: many little nicks but had one biggie in '86 - C-profile in a sliding 'tome - felt the knock and just knew i had done serious damage dashed to the sink with the other hand clutching the injured finger tightly - reluctant to open and look - funny that it did not hurt when it happened - just a hard knock with a sting - hurt like mad afterwards had 9 stitches and still have the scar to prove it i alway say that a serious cut is probably the only thing that forces a healthy respect for the blade very annoying to work in AP with a bandaged finger!!! Annie 2008/10/28 Horn, Hazel V > I've had stitches twice. Once 34 years ago, I rolled my thumb between > the block and the blade. (from rolling my thumb over the block to warm > it a bit as I was turning the wheel of the microtome.....didn't do that > anymore!!) And once 21 years ago when I was 9 months pregnant I > slashed my little finger on the end of the blade... > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor > Tobias > Sent: Monday, October 27, 2008 3:50 PM > To: Jackie M O'Connor > Cc: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Protect fingers during Microtomy > > Yes, nicked the corner of a steel blade reaching for slide. This was > back in 78 or 79. It was a Saturday, working by myself and I had to wait > > for 3 hours in the ER. No preferential treatment for employees in a > county hospital. > > Victor > > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > > Jackie M O'Connor wrote: > > OK - time to poll - who has had sutures from microtomy? Me! 23 > years > > ago - 8 months pregnant, and not paying attention to what I was doing. > Oh > > yeah, and one other time, I cut off the tip of my finger beta testing > a > > microtome safety invention from EHS. > > > > > > > > > > > > Peter Carroll > > Sent by: histonet-bounces@lists.utsouthwestern.edu > > 10/27/2008 03:17 PM > > > > To > > > > cc > > histonet@lists.utsouthwestern.edu > > Subject > > Re: [Histonet] Protect fingers during Microtomy > > > > > > > > > > > > > > > I am looking some think (like gloves)to protect the fingers during > > microtomy > > > > No offense, but if you're that prone to injury, I recommend steering > > clear of microtomes in general ;) > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and > confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your > computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From brett_connolly <@t> merck.com Tue Oct 28 08:10:49 2008 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Oct 28 08:11:17 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: References: <490621C6.60304@umdnj.edu> Message-ID: <63EA0607835FBA4689CEA9EA8B4826920173B5C0@usctmx1141.merck.com> Me - 1982 - working in a S. Florida hospital I sliced open the base of my thumb on the corner of a big steel blade as I was sectioning. Didn't even notice it until I saw the blood dripping off my elbow. I went to the ER and was sitting on a gurney with my hand submerged in a bowl of betadine waiting to be stitched up. After what seemed like a pretty long time, I deftly fell off the gurney onto the floor tipping the entire bowl all over onto myself. Upon hearing the clamor of me hitting the floor and the metal bowl banging around a rush of ER staff charged in and I got prompt service thereafter. I still have the scar, but I never cut myself again :-) Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, October 27, 2008 4:44 PM To: Peter Carroll Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Protect fingers during Microtomy OK - time to poll - who has had sutures from microtomy? Me! 23 years ago - 8 months pregnant, and not paying attention to what I was doing. Oh yeah, and one other time, I cut off the tip of my finger beta testing a microtome safety invention from EHS. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 10/27/2008 03:17 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Protect fingers during Microtomy > I am looking some think (like gloves)to protect the fingers during microtomy No offense, but if you're that prone to injury, I recommend steering clear of microtomes in general ;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From TELGENHOFF <@t> tarleton.edu Tue Oct 28 08:17:51 2008 From: TELGENHOFF <@t> tarleton.edu (Telgenhoff, Dr. Dale) Date: Tue Oct 28 08:20:59 2008 Subject: [Histonet] Protect fingers during Microtomy References: <490621C6.60304@umdnj.edu> <63EA0607835FBA4689CEA9EA8B4826920173B5C0@usctmx1141.merck.com> Message-ID: <5CEA6CACB9ABD74881E453BE9D966F24020BE306@exchange01.tarleton.edu> Not me, but the guy who trained me. Remember the old knife sharpeners that looked like record players? He was removing the blade (they got pretty oily) and it slipped. His natural reaction was to grab for it, slicing three fingers to the bone. Made for a good cautionary tale... ~~~~~~~~~~~~~~~~~~~~ Dale Telgenhoff, PhD Assistant Professor Tarleton State University Clinical Laboratory Science 1501 Enderly Place Fort Worth, TX 76104 telgenhoff@tarleton.edu 817-926-1101 Ext. 7 ~~~~~~~~~~~~~~~~~~~~ ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Connolly, Brett M Sent: Tue 10/28/2008 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Me - 1982 - working in a S. Florida hospital I sliced open the base of my thumb on the corner of a big steel blade as I was sectioning. Didn't even notice it until I saw the blood dripping off my elbow. I went to the ER and was sitting on a gurney with my hand submerged in a bowl of betadine waiting to be stitched up. After what seemed like a pretty long time, I deftly fell off the gurney onto the floor tipping the entire bowl all over onto myself. Upon hearing the clamor of me hitting the floor and the metal bowl banging around a rush of ER staff charged in and I got prompt service thereafter. I still have the scar, but I never cut myself again :-) Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, October 27, 2008 4:44 PM To: Peter Carroll Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Protect fingers during Microtomy OK - time to poll - who has had sutures from microtomy? Me! 23 years ago - 8 months pregnant, and not paying attention to what I was doing. Oh yeah, and one other time, I cut off the tip of my finger beta testing a microtome safety invention from EHS. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 10/27/2008 03:17 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Protect fingers during Microtomy > I am looking some think (like gloves)to protect the fingers during microtomy No offense, but if you're that prone to injury, I recommend steering clear of microtomes in general ;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Oct 28 08:27:35 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Oct 28 08:27:45 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <5CEA6CACB9ABD74881E453BE9D966F24020BE306@exchange01.tarleton.edu> References: <490621C6.60304@umdnj.edu><63EA0607835FBA4689CEA9EA8B4826920173B5C0@usctmx1141.merck.com> <5CEA6CACB9ABD74881E453BE9D966F24020BE306@exchange01.tarleton.edu> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA50E4089@ITSSSXM01V6.one.ads.che.org> I've heard that tale, but didn't know who did it! I had a coworked who cut the pad off his thumb. We went to the ER, where I learned he had put it in the trash. I went back and dug through the paraffin scraps and got it - washed it in saline and Betadine.. The ER dr glued it back on and he has a thumbprint again! When I caught myself on fire, cleaning with solvants too close to the hot plate (the fumes ignited), he was the same guy who yelled, "Get the marshmallows - the supervisor's on fire!". I still get a good laugh about that! J:>) And it's only Tues!! Trick or treat!!! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Telgenhoff, Dr. Dale Sent: Tuesday, October 28, 2008 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Not me, but the guy who trained me. Remember the old knife sharpeners that looked like record players? He was removing the blade (they got pretty oily) and it slipped. His natural reaction was to grab for it, slicing three fingers to the bone. Made for a good cautionary tale... ~~~~~~~~~~~~~~~~~~~~ Dale Telgenhoff, PhD Assistant Professor Tarleton State University Clinical Laboratory Science 1501 Enderly Place Fort Worth, TX 76104 telgenhoff@tarleton.edu 817-926-1101 Ext. 7 ~~~~~~~~~~~~~~~~~~~~ ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Connolly, Brett M Sent: Tue 10/28/2008 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Me - 1982 - working in a S. Florida hospital I sliced open the base of my thumb on the corner of a big steel blade as I was sectioning. Didn't even notice it until I saw the blood dripping off my elbow. I went to the ER and was sitting on a gurney with my hand submerged in a bowl of betadine waiting to be stitched up. After what seemed like a pretty long time, I deftly fell off the gurney onto the floor tipping the entire bowl all over onto myself. Upon hearing the clamor of me hitting the floor and the metal bowl banging around a rush of ER staff charged in and I got prompt service thereafter. I still have the scar, but I never cut myself again :-) Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, October 27, 2008 4:44 PM To: Peter Carroll Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Protect fingers during Microtomy OK - time to poll - who has had sutures from microtomy? Me! 23 years ago - 8 months pregnant, and not paying attention to what I was doing. Oh yeah, and one other time, I cut off the tip of my finger beta testing a microtome safety invention from EHS. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 10/27/2008 03:17 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Protect fingers during Microtomy > I am looking some think (like gloves)to protect the fingers during microtomy No offense, but if you're that prone to injury, I recommend steering clear of microtomes in general ;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Jackie.O'Connor <@t> abbott.com Tue Oct 28 08:38:18 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Oct 28 08:38:44 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <5CEA6CACB9ABD74881E453BE9D966F24020BE306@exchange01.tarleton.edu> Message-ID: My Dad was a workman's comp expert for a large insurance company. In the early 90's, I was managing a histo lab where the head pathologist (funny, a neuropath) thought it would be cheaper to resharpen stainless steel microtome knives instead of using disposable blades.(I had a previous nasty injury from a steel knife). Dad put together an estimate on each individual finger of how much $$ the hospital would have to pay out in the event of major lacerations or amputation. We also included feet - since those heavy knives would often hit the floor when you had to jump out of the way if someone mishandled one. Turned out, we never used stainless steel knives again. It was cheaper to use disposables than for one person to lose the use of a thumb. From lblazek <@t> digestivespecialists.com Tue Oct 28 08:48:28 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Oct 28 08:47:22 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA50E4089@ITSSSXM01V6.one.ads.che.org> References: <490621C6.60304@umdnj.edu><63EA0607835FBA4689CEA9EA8B4826920173B5C0@usctmx1141.merck.com> <5CEA6CACB9ABD74881E453BE9D966F24020BE306@exchange01.tarleton.edu> <5D64396A0D4A5346BEBC759022AAEAA50E4089@ITSSSXM01V6.one.ads.che.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E3907B8F86843@IBMB7Exchange.digestivespecialists.com> Besides a few little nicks that we all get, about 10 years ago after having worked in histology for almost 25 years I was finally in a lab with windows! Early one morning as I was sitting there cutting with my back to the window, dawn just breaking. A head popped up outside the window and the tech facing the window screamed just as I was running my thumb over the block. I jumped; the chuck came down and sliced into the pad of my thumb down to the bone. I ran to the sink holding my thumb and ran it under water. It's pretty gross to see the bone in your thumb! Funny thing, there was no pain until the ER doc filled it full of the stuff to numb it. Got lots of stitches and even now it sometimes hurts. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, October 28, 2008 9:28 AM To: Telgenhoff, Dr. Dale; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy I've heard that tale, but didn't know who did it! I had a coworked who cut the pad off his thumb. We went to the ER, where I learned he had put it in the trash. I went back and dug through the paraffin scraps and got it - washed it in saline and Betadine.. The ER dr glued it back on and he has a thumbprint again! When I caught myself on fire, cleaning with solvants too close to the hot plate (the fumes ignited), he was the same guy who yelled, "Get the marshmallows - the supervisor's on fire!". I still get a good laugh about that! J:>) And it's only Tues!! Trick or treat!!! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Telgenhoff, Dr. Dale Sent: Tuesday, October 28, 2008 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Not me, but the guy who trained me. Remember the old knife sharpeners that looked like record players? He was removing the blade (they got pretty oily) and it slipped. His natural reaction was to grab for it, slicing three fingers to the bone. Made for a good cautionary tale... ~~~~~~~~~~~~~~~~~~~~ Dale Telgenhoff, PhD Assistant Professor Tarleton State University Clinical Laboratory Science 1501 Enderly Place Fort Worth, TX 76104 telgenhoff@tarleton.edu 817-926-1101 Ext. 7 ~~~~~~~~~~~~~~~~~~~~ ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Connolly, Brett M Sent: Tue 10/28/2008 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Me - 1982 - working in a S. Florida hospital I sliced open the base of my thumb on the corner of a big steel blade as I was sectioning. Didn't even notice it until I saw the blood dripping off my elbow. I went to the ER and was sitting on a gurney with my hand submerged in a bowl of betadine waiting to be stitched up. After what seemed like a pretty long time, I deftly fell off the gurney onto the floor tipping the entire bowl all over onto myself. Upon hearing the clamor of me hitting the floor and the metal bowl banging around a rush of ER staff charged in and I got prompt service thereafter. I still have the scar, but I never cut myself again :-) Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, October 27, 2008 4:44 PM To: Peter Carroll Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Protect fingers during Microtomy OK - time to poll - who has had sutures from microtomy? Me! 23 years ago - 8 months pregnant, and not paying attention to what I was doing. Oh yeah, and one other time, I cut off the tip of my finger beta testing a microtome safety invention from EHS. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 10/27/2008 03:17 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Protect fingers during Microtomy > I am looking some think (like gloves)to protect the fingers during microtomy No offense, but if you're that prone to injury, I recommend steering clear of microtomes in general ;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Tue Oct 28 09:22:52 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Oct 28 09:23:00 2008 Subject: [Histonet] Re: Uranyl nitrate Message-ID: I certainly agree that uranyl nitrate is better got out of the laboratory - not only is it radioactive, but it's a strong oxidant. I suppose that nowadays it's made from depleted uranium (uranium from which most of the U235 has been isotopically separated), but uranium 238 slowly decays into radium 226, and as the radium accumulates the stuff in the bottle becomes more radioactive. You can get around the disposal problem in the short term by precipitating the uranium as sodium uranyl sulfate, which is quite insoluble and can be stored in a jar under the sink. By the time the jar is full... well, I was going to say, you'll be retired, but I've quit saying that these days. - I can probably find the formula for doing this if anybody's interested. I remember a laboratory exercise my freshman year in college that involved co-precipitating the radium with ferric hydroxide, and demonstrating with a Geiger counter that almost all the radioactivity was in the precipitate, which actually (even in 1955) had to be disposed of as radioactive waste. I recall my lab partner's notebook contained the unfortunate misspelling "urinal nitrate" which the lab section man had circled and noted "Oh no!" - The section man, Addison Ault, went on to become the author of the foremost college organic chemistry textbook of its time. But an old man digresses. Bob Richmond Samurai Pathologist Knoxville TN From Terry.Marshall <@t> rothgen.nhs.uk Tue Oct 28 10:24:25 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Oct 28 10:24:38 2008 Subject: [Histonet] Re: Uranyl nitrate References: Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F56E@TRFT-EX01.xRothGen.nhs.uk> What a wonderful post. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: 28 October 2008 14:23 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Uranyl nitrate I certainly agree that uranyl nitrate is better got out of the laboratory - not only is it radioactive, but it's a strong oxidant. I suppose that nowadays it's made from depleted uranium (uranium from which most of the U235 has been isotopically separated), but uranium 238 slowly decays into radium 226, and as the radium accumulates the stuff in the bottle becomes more radioactive. You can get around the disposal problem in the short term by precipitating the uranium as sodium uranyl sulfate, which is quite insoluble and can be stored in a jar under the sink. By the time the jar is full... well, I was going to say, you'll be retired, but I've quit saying that these days. - I can probably find the formula for doing this if anybody's interested. I remember a laboratory exercise my freshman year in college that involved co-precipitating the radium with ferric hydroxide, and demonstrating with a Geiger counter that almost all the radioactivity was in the precipitate, which actually (even in 1955) had to be disposed of as radioactive waste. I recall my lab partner's notebook contained the unfortunate misspelling "urinal nitrate" which the lab section man had circled and noted "Oh no!" - The section man, Addison Ault, went on to become the author of the foremost college organic chemistry textbook of its time. But an old man digresses. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Tue Oct 28 10:26:42 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Oct 28 10:26:47 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <5A2BD13465E061429D6455C8D6B40E3907B8F86843@IBMB7Exchange.d igestivespecialists.com> References: <490621C6.60304@umdnj.edu> <63EA0607835FBA4689CEA9EA8B4826920173B5C0@usctmx1141.merck.com> <5CEA6CACB9ABD74881E453BE9D966F24020BE306@exchange01.tarleton.edu> <5D64396A0D4A5346BEBC759022AAEAA50E4089@ITSSSXM01V6.one.ads.che.org> <5A2BD13465E061429D6455C8D6B40E3907B8F86843@IBMB7Exchange.digestivespecialists.com> Message-ID: <6.2.3.4.1.20081028081547.026eaeb0@algranth.inbox.email.arizona.edu> EWWWWW! All these accounts give me the heebiejeebies. I have had a few nonserious cuts - just yesterday when I was answering the phone for the third time with the same customer while cleaning up the microtome as a matter of fact. I'm now sporting a barbie bandaid! But a few years ago one of the students who didn't listen when I gave the lecture on putting on the brake when changing blocks did a fantastic job of cutting off the end of her thumb down to the bone. Thankfully another of my student workers was a training to be a EMT and a medic in the reserves and he took care of the immediate washing and wrapping so we could get her to the ER. She was out of commission for a long time, had to have physical therapy and still had a lot of pain last time I saw her. Andi At 06:48 AM 10/28/2008, Blazek, Linda wrote: >Besides a few little nicks that we all get, about 10 years ago after >having worked in histology for almost 25 years I was finally in a >lab with windows! Early one morning as I was sitting there cutting >with my back to the window, dawn just breaking. A head popped up >outside the window and the tech facing the window screamed just as I >was running my thumb over the block. I jumped; the chuck came down >and sliced into the pad of my thumb down to the bone. I ran to the >sink holding my thumb and ran it under water. It's pretty gross to >see the bone in your thumb! Funny thing, there was no pain until >the ER doc filled it full of the stuff to numb it. Got lots of >stitches and even now it sometimes hurts. > >Linda Blazek HT (ASCP) >Manager/Supervisor >GI Pathology of Dayton >7415 Brandt Pike >Huber Heights, OH 45424 >Phone: (937) 293-4424 ext 7118 >Email: lblazek@digestivespecialists.com > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce >Sent: Tuesday, October 28, 2008 9:28 AM >To: Telgenhoff, Dr. Dale; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Protect fingers during Microtomy > >I've heard that tale, but didn't know who did it! > >I had a coworked who cut the pad off his thumb. We went to the ER, where >I learned he had put it in the trash. I went back and dug through the >paraffin scraps and got it - washed it in saline and Betadine.. The ER >dr glued it back on and he has a thumbprint again! > >When I caught myself on fire, cleaning with solvants too close to the >hot plate (the fumes ignited), he was the same guy who yelled, "Get the >marshmallows - the supervisor's on fire!". I still get a good laugh >about that! J:>) > >And it's only Tues!! Trick or treat!!! > > > >Joyce Weems >Pathology Manager >Saint Joseph's Hospital >5665 Peachtree Dunwoody Rd NE >Atlanta, GA 30342 >Please note new phone and fax numbers >678-843-7376 - Phone >678-843-7831 - Fax > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Telgenhoff, Dr. Dale >Sent: Tuesday, October 28, 2008 9:18 AM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Protect fingers during Microtomy > >Not me, but the guy who trained me. Remember the old knife sharpeners >that looked like record players? He was removing the blade (they got >pretty oily) and it slipped. His natural reaction was to grab for it, >slicing three fingers to the bone. Made for a good cautionary tale... > >~~~~~~~~~~~~~~~~~~~~ >Dale Telgenhoff, PhD >Assistant Professor >Tarleton State University >Clinical Laboratory Science >1501 Enderly Place >Fort Worth, TX 76104 >telgenhoff@tarleton.edu >817-926-1101 Ext. 7 >~~~~~~~~~~~~~~~~~~~~ > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Connolly, >Brett M >Sent: Tue 10/28/2008 8:10 AM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Protect fingers during Microtomy > > > > > Me - 1982 - working in a S. Florida hospital I sliced open the base of >my thumb on the corner of a big steel blade as I was sectioning. Didn't >even notice it until I saw the blood dripping off my elbow. > >I went to the ER and was sitting on a gurney with my hand submerged in a >bowl of betadine waiting to be stitched up. After what seemed like a >pretty long time, I deftly fell off the gurney onto the floor tipping >the entire bowl all over onto myself. > >Upon hearing the clamor of me hitting the floor and the metal bowl >banging around a rush of ER staff charged in and I got prompt service >thereafter. I still have the scar, but I never cut myself again :-) > >Brett > >Brett M. Connolly, Ph.D. >Research Fellow, Imaging Research >Merck & Co., Inc. >PO Box 4, WP-44K >West Point, PA 19486 >PH 215-652-2501 fax. 215-993-6803 >e-mail. brett_connolly@merck.com > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M >O'Connor >Sent: Monday, October 27, 2008 4:44 PM >To: Peter Carroll >Cc: histonet@lists.utsouthwestern.edu; >histonet-bounces@lists.utsouthwestern.edu >Subject: Re: [Histonet] Protect fingers during Microtomy > >OK - time to poll - who has had sutures from microtomy? Me! 23 years > >ago - 8 months pregnant, and not paying attention to what I was doing. >Oh >yeah, and one other time, I cut off the tip of my finger beta testing a >microtome safety invention from EHS. > > > > > >Peter Carroll >Sent by: histonet-bounces@lists.utsouthwestern.edu >10/27/2008 03:17 PM > >To > >cc >histonet@lists.utsouthwestern.edu >Subject >Re: [Histonet] Protect fingers during Microtomy > > > > > > > > I am looking some think (like gloves)to protect the fingers during >microtomy > >No offense, but if you're that prone to injury, I recommend steering >clear of microtomes in general ;) > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Notice: This e-mail message, together with any attachments, contains >information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, >New Jersey, USA 08889), and/or its affiliates (which may be known >outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD >and in Japan, as Banyu - direct contact information for affiliates is >available at http://www.merck.com/contact/contacts.html) that may be >confidential, proprietary copyrighted and/or legally privileged. It is >intended solely for the use of the individual or entity named on this >message. If you are not the intended recipient, and have received this >message in error, please notify us immediately by reply e-mail and then >delete it from your system. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Confidentiality Notice: >This email, including any attachments is the >property of Catholic Health East and is intended >for the sole use of the intended recipient(s). >It may contain information that is privileged and >confidential. Any unauthorized review, use, >disclosure, or distribution is prohibited. If you are >not the intended recipient, please reply to the >sender that you have received the message in >error, then delete this message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From mbisher <@t> Princeton.EDU Tue Oct 28 10:34:00 2008 From: mbisher <@t> Princeton.EDU (Peggy Bisher) Date: Tue Oct 28 10:34:21 2008 Subject: [Histonet] Histology and immuonhistochemistry of Drosophila eyesections (OT) In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A120192@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: I have students here who have been doing cryo-sectioning of Drosophila. No one, has yet tried it in paraffin. Since they are very tiny flies, we have a contraption that allows us to orient the flies so that their heads are all in a row and we just cut a whole bunch at once. It looks like a slide with a long groove in it - just wide enough to fit their heads above it and the bodies hang down below. It seems to work just fine. Cheers, Peggy Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher@princeton.edu On 10/27/08 9:46 PM, "Ingles Claire" wrote: > > Fly's eyes? What about bee's knees? > Sorry it's almost 9pm. I'm fizzling out. > Claire :o > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of pam johnson > Sent: Mon 10/27/2008 4:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology and immuonhistochemistry of Drosophila > eyesections > > > > > I have been approached by an investigator?who wants us to do some work on fly > eyes. Has anyone had any experience with fly eyes in paraffin? If so can you > please share your protocols on processing and cutting. Aren't they very > crunchy? Do you process the whole head? > > ? > > Any information would be greatly appreciated. > > Thanks, > > > Pam Johnson, BS, HT (ASCP) > Lab Manager > Veterinary Pathology Core Lab > St. Jude Children's Research Hospital > 262 Danny Thomas Place > Memphis, TN? 38105-3678 > Office - 901-595-3355 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Oct 28 11:01:37 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Oct 28 11:02:00 2008 Subject: [Histonet] processing and sectioning silicon? Message-ID: Hi all, Has anyone experience with processing and cutting silicon? We have got a silicon-scaffold with grown cells on it. They want us to embed it in paraffin. I would be happy about any input. Gudrun Lang Histolabor, Akh Linz, Austria From jfish <@t> gladstone.ucsf.edu Tue Oct 28 11:14:10 2008 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Tue Oct 28 11:14:13 2008 Subject: [Histonet] Histology and immuonhistochemistry of Drosophilaeyesections (OT) In-Reply-To: References: <08A0A863637F1349BBFD83A96B27A50A120192@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <360CDBD7A0FA4702B64BE41C34E8D753@JFISH> Hi Pam, I have worked with the little guys for a while now. The best way is to pre-embed in agar or histogel (although I have had a lot of bad luck with the histogel getting "crispy" in our processor). Put a small amount of agar in a small mold, like the tissue tek plastic molds, then align the flies in the orientation that you would like. Then just process the entire block of agar into paraffin and embed. They cut beautifully. Good luck, Jo Dee ~~Jo Dee Fish~~ Senior Research Technologist The J. David Gladstone Institutes Co-manager Histology and Microscopy Core Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peggy Bisher Sent: Tuesday, October 28, 2008 8:34 AM To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histology and immuonhistochemistry of Drosophilaeyesections (OT) I have students here who have been doing cryo-sectioning of Drosophila. No one, has yet tried it in paraffin. Since they are very tiny flies, we have a contraption that allows us to orient the flies so that their heads are all in a row and we just cut a whole bunch at once. It looks like a slide with a long groove in it - just wide enough to fit their heads above it and the bodies hang down below. It seems to work just fine. Cheers, Peggy Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher@princeton.edu On 10/27/08 9:46 PM, "Ingles Claire" wrote: > > Fly's eyes? What about bee's knees? > Sorry it's almost 9pm. I'm fizzling out. > Claire :o > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of pam > johnson > Sent: Mon 10/27/2008 4:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology and immuonhistochemistry of Drosophila > eyesections > > > > > I have been approached by an investigator?who wants us to do some work > on fly eyes. Has anyone had any experience with fly eyes in paraffin? > If so can you please share your protocols on processing and cutting. > Aren't they very crunchy? Do you process the whole head? > > ? > > Any information would be greatly appreciated. > > Thanks, > > > Pam Johnson, BS, HT (ASCP) > Lab Manager > Veterinary Pathology Core Lab > St. Jude Children's Research Hospital > 262 Danny Thomas Place > Memphis, TN? 38105-3678 > Office - 901-595-3355 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Tue Oct 28 11:18:32 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Oct 28 11:18:43 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <5A2BD13465E061429D6455C8D6B40E3907B8F86843@IBMB7Exchange.digestivespecialists.com> Message-ID: <000201c93918$d215c450$d00f7ca5@lurie.northwestern.edu> I always thought we weren't truly a tech until we did actually cut ourselves!!!! I got myself wiping the filings off a blade I had just sharpened on the Hacker sharpener.My thumb slide up and over as I was wiping. Even with the paper towel it cut and hurt. I think the paper towel saved me from having stitches. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, October 28, 2008 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Besides a few little nicks that we all get, about 10 years ago after having worked in histology for almost 25 years I was finally in a lab with windows! Early one morning as I was sitting there cutting with my back to the window, dawn just breaking. A head popped up outside the window and the tech facing the window screamed just as I was running my thumb over the block. I jumped; the chuck came down and sliced into the pad of my thumb down to the bone. I ran to the sink holding my thumb and ran it under water. It's pretty gross to see the bone in your thumb! Funny thing, there was no pain until the ER doc filled it full of the stuff to numb it. Got lots of stitches and even now it sometimes hurts. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, October 28, 2008 9:28 AM To: Telgenhoff, Dr. Dale; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy I've heard that tale, but didn't know who did it! I had a coworked who cut the pad off his thumb. We went to the ER, where I learned he had put it in the trash. I went back and dug through the paraffin scraps and got it - washed it in saline and Betadine.. The ER dr glued it back on and he has a thumbprint again! When I caught myself on fire, cleaning with solvants too close to the hot plate (the fumes ignited), he was the same guy who yelled, "Get the marshmallows - the supervisor's on fire!". I still get a good laugh about that! J:>) And it's only Tues!! Trick or treat!!! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Telgenhoff, Dr. Dale Sent: Tuesday, October 28, 2008 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Not me, but the guy who trained me. Remember the old knife sharpeners that looked like record players? He was removing the blade (they got pretty oily) and it slipped. His natural reaction was to grab for it, slicing three fingers to the bone. Made for a good cautionary tale... ~~~~~~~~~~~~~~~~~~~~ Dale Telgenhoff, PhD Assistant Professor Tarleton State University Clinical Laboratory Science 1501 Enderly Place Fort Worth, TX 76104 telgenhoff@tarleton.edu 817-926-1101 Ext. 7 ~~~~~~~~~~~~~~~~~~~~ ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Connolly, Brett M Sent: Tue 10/28/2008 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Me - 1982 - working in a S. Florida hospital I sliced open the base of my thumb on the corner of a big steel blade as I was sectioning. Didn't even notice it until I saw the blood dripping off my elbow. I went to the ER and was sitting on a gurney with my hand submerged in a bowl of betadine waiting to be stitched up. After what seemed like a pretty long time, I deftly fell off the gurney onto the floor tipping the entire bowl all over onto myself. Upon hearing the clamor of me hitting the floor and the metal bowl banging around a rush of ER staff charged in and I got prompt service thereafter. I still have the scar, but I never cut myself again :-) Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, October 27, 2008 4:44 PM To: Peter Carroll Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Protect fingers during Microtomy OK - time to poll - who has had sutures from microtomy? Me! 23 years ago - 8 months pregnant, and not paying attention to what I was doing. Oh yeah, and one other time, I cut off the tip of my finger beta testing a microtome safety invention from EHS. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 10/27/2008 03:17 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Protect fingers during Microtomy > I am looking some think (like gloves)to protect the fingers during microtomy No offense, but if you're that prone to injury, I recommend steering clear of microtomes in general ;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juditw <@t> u.washington.edu Tue Oct 28 11:26:55 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Tue Oct 28 11:27:05 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA50E4089@ITSSSXM01V6.one.ads.che.org> Message-ID: Hey- Are all you young "in training" histotechs out there listening and learning???:-) yes, and we walked up hill in the snow to work!! (both ways- and sharpened our knives by HAND -eeeewww- how quaint!) Judy in Washington On Tue, 28 Oct 2008, Weems, Joyce wrote: > I've heard that tale, but didn't know who did it! > > I had a coworked who cut the pad off his thumb. We went to the ER, where > I learned he had put it in the trash. I went back and dug through the > paraffin scraps and got it - washed it in saline and Betadine.. The ER > dr glued it back on and he has a thumbprint again! > > When I caught myself on fire, cleaning with solvants too close to the > hot plate (the fumes ignited), he was the same guy who yelled, "Get the > marshmallows - the supervisor's on fire!". I still get a good laugh > about that! J:>) > > And it's only Tues!! Trick or treat!!! > > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > Please note new phone and fax numbers > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Telgenhoff, Dr. Dale > Sent: Tuesday, October 28, 2008 9:18 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Protect fingers during Microtomy > > Not me, but the guy who trained me. Remember the old knife sharpeners > that looked like record players? He was removing the blade (they got > pretty oily) and it slipped. His natural reaction was to grab for it, > slicing three fingers to the bone. Made for a good cautionary tale... > > ~~~~~~~~~~~~~~~~~~~~ > Dale Telgenhoff, PhD > Assistant Professor > Tarleton State University > Clinical Laboratory Science > 1501 Enderly Place > Fort Worth, TX 76104 > telgenhoff@tarleton.edu > 817-926-1101 Ext. 7 > ~~~~~~~~~~~~~~~~~~~~ > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Connolly, > Brett M > Sent: Tue 10/28/2008 8:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Protect fingers during Microtomy > > > > > Me - 1982 - working in a S. Florida hospital I sliced open the base of > my thumb on the corner of a big steel blade as I was sectioning. Didn't > even notice it until I saw the blood dripping off my elbow. > > I went to the ER and was sitting on a gurney with my hand submerged in a > bowl of betadine waiting to be stitched up. After what seemed like a > pretty long time, I deftly fell off the gurney onto the floor tipping > the entire bowl all over onto myself. > > Upon hearing the clamor of me hitting the floor and the metal bowl > banging around a rush of ER staff charged in and I got prompt service > thereafter. I still have the scar, but I never cut myself again :-) > > Brett > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Research > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > PH 215-652-2501 fax. 215-993-6803 > e-mail. brett_connolly@merck.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M > O'Connor > Sent: Monday, October 27, 2008 4:44 PM > To: Peter Carroll > Cc: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Protect fingers during Microtomy > > OK - time to poll - who has had sutures from microtomy? Me! 23 years > > ago - 8 months pregnant, and not paying attention to what I was doing. > Oh > yeah, and one other time, I cut off the tip of my finger beta testing a > microtome safety invention from EHS. > > > > > > Peter Carroll > Sent by: histonet-bounces@lists.utsouthwestern.edu > 10/27/2008 03:17 PM > > To > > cc > histonet@lists.utsouthwestern.edu > Subject > Re: [Histonet] Protect fingers during Microtomy > > > > > > > > I am looking some think (like gloves)to protect the fingers during > microtomy > > No offense, but if you're that prone to injury, I recommend steering > clear of microtomes in general ;) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates (which may be known > outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD > and in Japan, as Banyu - direct contact information for affiliates is > available at http://www.merck.com/contact/contacts.html) that may be > confidential, proprietary copyrighted and/or legally privileged. It is > intended solely for the use of the individual or entity named on this > message. If you are not the intended recipient, and have received this > message in error, please notify us immediately by reply e-mail and then > delete it from your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From jhnspam <@t> aol.com Tue Oct 28 11:30:16 2008 From: jhnspam <@t> aol.com (pam johnson) Date: Tue Oct 28 11:30:34 2008 Subject: [Histonet] PAS with Glycogen Digestion - Solution Not Working In-Reply-To: References: <816E3C72F855F14985FC31D7C963AE6F0AE51629@indexch03.ent.covance.com><981878.53101.qm@web65702.mail.ac4.yahoo.com> Message-ID: <8CB0731981D535A-FE4-452@FWM-M17.sysops.aol.com> What is wrong with saliva. I have done this procedure for 20 years. It works beautifully. -----Original Message----- From: Mickie Johnson To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'McKnight, Tanisha' Sent: Sun, 26 Oct 2008 9:09 am Subject: RE: [Histonet] PAS with Glycogen Digestion - Solution Not Working Diastase powder will dissolve in any water, but requires some calcium ions o function. Thus it will not perform in distilled water, but will perform ell using plain tap water. Also, the diastase powder from sigma will work ell if only a very small amount of the powder is used. We always used just nough to barely cover the bottom of a glass coplin jar and then fill with ap water. I would expect 0.5 grams in 50 mls of water is probably too much. ry using one tenth that amount or 0.05 gm in 50 ml. When there is too much iastase in solution, enzyme conformation is inhibited and it does not ork. Spokane water is quite hard. It always worked well at room temperature or 20 minutes. Mickie ickie Johnson, B.S., HTL(ASCP) ohs Histology Consulting Services, LLC & Mohs Lab Staffing 507 S. Manito Blvd. pokane, WA 99203 09-954-7134 AX 509-624-3926 eb: www.mohshistogyconsulting.com & www.mohslabstaffing.com mail: mickie25@netzero.net ISCLAIMER: his message is intended for the sole use of the addressee, and may contain nformation that is privileged, confidential and exempt from disclosure nder applicable law. If you are not the addressee you are hereby notified hat you may not use, copy, disclose, or distribute to anyone the message or ny information contained in the message. If you have received this message n error, please immediately advise the sender by reply email and delete his message. ----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa ent: Friday, October 24, 2008 8:38 AM o: histonet@lists.utsouthwestern.edu; McKnight, Tanisha ubject: Re: [Histonet] PAS with Glycogen Digestion - Solution Not Working Tanisha: eing an enzyme, you cannot use diastase in distilled water. You have to repare a diastase buffer at pH7 to dissolve the diastase and do the igestion at 37?C during 30 minutes. That will work. lease, do not try to use saliva! en? J. --- On Fri, 10/24/08, McKnight, Tanisha rote: From: McKnight, Tanisha ubject: [Histonet] PAS with Glycogen Digestion - Solution Not Working o: histonet@lists.utsouthwestern.edu ate: Friday, October 24, 2008, 11:26 AM Hi All: am in the process of working up a PAS Staining protocol. The PAS stain s working fine, but I am unable to get my tissue to digest. I am using 5g of Diastase in 50ml of DI water. I tried heating the solution in a ater bath for 30 min, 1 hour and 1.5 hours and it still looks the same s the one without digestion. Could I be using the wrong Diastase? If o, does anyone have any suggestions for a new product? hanks, anisha Neely, HT(ASCP) P-Histology/Specimen Management ? ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. hecked by AVG - http://www.avg.com ersion: 8.0.175 / Virus Database: 270.8.3/1747 - Release Date: 10/26/2008 :27 AM ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Tue Oct 28 11:54:41 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Oct 28 11:55:09 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <5A2BD13465E061429D6455C8D6B40E3907B8F86843@IBMB7Exchange.digestivespecialists.com> References: <490621C6.60304@umdnj.edu><63EA0607835FBA4689CEA9EA8B4826920173B5C0@usctmx1141.merck.com> <5CEA6CACB9ABD74881E453BE9D966F24020BE306@exchange01.tarleton.edu> <5D64396A0D4A5346BEBC759022AAEAA50E4089@ITSSSXM01V6.one.ads.che.org> <5A2BD13465E061429D6455C8D6B40E3907B8F86843@IBMB7Exchange.digestivespecialists.com> Message-ID: <7722595275A4DD4FA225B92CDBF174A1744CF0B3C7@EXC-MBX3.cfs.le.ac.uk> My wife, the best section cutter in the world worked right up to the bitter end of her pregnancy with our third daughter, dropped an old style steel knife and inadvertently gave herself a caesarean, beat that histopeople!!!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: 28 October 2008 13:48 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Besides a few little nicks that we all get, about 10 years ago after having worked in histology for almost 25 years I was finally in a lab with windows! Early one morning as I was sitting there cutting with my back to the window, dawn just breaking. A head popped up outside the window and the tech facing the window screamed just as I was running my thumb over the block. I jumped; the chuck came down and sliced into the pad of my thumb down to the bone. I ran to the sink holding my thumb and ran it under water. It's pretty gross to see the bone in your thumb! Funny thing, there was no pain until the ER doc filled it full of the stuff to numb it. Got lots of stitches and even now it sometimes hurts. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, October 28, 2008 9:28 AM To: Telgenhoff, Dr. Dale; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy I've heard that tale, but didn't know who did it! I had a coworked who cut the pad off his thumb. We went to the ER, where I learned he had put it in the trash. I went back and dug through the paraffin scraps and got it - washed it in saline and Betadine.. The ER dr glued it back on and he has a thumbprint again! When I caught myself on fire, cleaning with solvants too close to the hot plate (the fumes ignited), he was the same guy who yelled, "Get the marshmallows - the supervisor's on fire!". I still get a good laugh about that! J:>) And it's only Tues!! Trick or treat!!! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Telgenhoff, Dr. Dale Sent: Tuesday, October 28, 2008 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Not me, but the guy who trained me. Remember the old knife sharpeners that looked like record players? He was removing the blade (they got pretty oily) and it slipped. His natural reaction was to grab for it, slicing three fingers to the bone. Made for a good cautionary tale... ~~~~~~~~~~~~~~~~~~~~ Dale Telgenhoff, PhD Assistant Professor Tarleton State University Clinical Laboratory Science 1501 Enderly Place Fort Worth, TX 76104 telgenhoff@tarleton.edu 817-926-1101 Ext. 7 ~~~~~~~~~~~~~~~~~~~~ ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Connolly, Brett M Sent: Tue 10/28/2008 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Me - 1982 - working in a S. Florida hospital I sliced open the base of my thumb on the corner of a big steel blade as I was sectioning. Didn't even notice it until I saw the blood dripping off my elbow. I went to the ER and was sitting on a gurney with my hand submerged in a bowl of betadine waiting to be stitched up. After what seemed like a pretty long time, I deftly fell off the gurney onto the floor tipping the entire bowl all over onto myself. Upon hearing the clamor of me hitting the floor and the metal bowl banging around a rush of ER staff charged in and I got prompt service thereafter. I still have the scar, but I never cut myself again :-) Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, October 27, 2008 4:44 PM To: Peter Carroll Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Protect fingers during Microtomy OK - time to poll - who has had sutures from microtomy? Me! 23 years ago - 8 months pregnant, and not paying attention to what I was doing. Oh yeah, and one other time, I cut off the tip of my finger beta testing a microtome safety invention from EHS. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 10/27/2008 03:17 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Protect fingers during Microtomy > I am looking some think (like gloves)to protect the fingers during microtomy No offense, but if you're that prone to injury, I recommend steering clear of microtomes in general ;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Oct 28 12:10:13 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Oct 28 12:10:26 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: References: <5D64396A0D4A5346BEBC759022AAEAA50E4089@ITSSSXM01V6.one.ads.che.org> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA50E411B@ITSSSXM01V6.one.ads.che.org> Barefoot, no less... -----Original Message----- From: Judith L. Williams [mailto:juditw@u.washington.edu] Sent: Tuesday, October 28, 2008 12:27 PM To: Weems, Joyce Cc: Telgenhoff, Dr. Dale; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Hey- Are all you young "in training" histotechs out there listening and learning???:-) yes, and we walked up hill in the snow to work!! (both ways- and sharpened our knives by HAND -eeeewww- how quaint!) Judy in Washington On Tue, 28 Oct 2008, Weems, Joyce wrote: > I've heard that tale, but didn't know who did it! > > I had a coworked who cut the pad off his thumb. We went to the ER, > where I learned he had put it in the trash. I went back and dug > through the paraffin scraps and got it - washed it in saline and > Betadine.. The ER dr glued it back on and he has a thumbprint again! > > When I caught myself on fire, cleaning with solvants too close to the > hot plate (the fumes ignited), he was the same guy who yelled, "Get > the marshmallows - the supervisor's on fire!". I still get a good > laugh about that! J:>) > > And it's only Tues!! Trick or treat!!! > > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > Please note new phone and fax numbers > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Telgenhoff, Dr. Dale > Sent: Tuesday, October 28, 2008 9:18 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Protect fingers during Microtomy > > Not me, but the guy who trained me. Remember the old knife sharpeners > that looked like record players? He was removing the blade (they got > pretty oily) and it slipped. His natural reaction was to grab for it, > slicing three fingers to the bone. Made for a good cautionary tale... > > ~~~~~~~~~~~~~~~~~~~~ > Dale Telgenhoff, PhD > Assistant Professor > Tarleton State University > Clinical Laboratory Science > 1501 Enderly Place > Fort Worth, TX 76104 > telgenhoff@tarleton.edu > 817-926-1101 Ext. 7 > ~~~~~~~~~~~~~~~~~~~~ > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Connolly, > Brett M > Sent: Tue 10/28/2008 8:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Protect fingers during Microtomy > > > > > Me - 1982 - working in a S. Florida hospital I sliced open the base of > my thumb on the corner of a big steel blade as I was sectioning. > Didn't even notice it until I saw the blood dripping off my elbow. > > I went to the ER and was sitting on a gurney with my hand submerged in > a bowl of betadine waiting to be stitched up. After what seemed like a > pretty long time, I deftly fell off the gurney onto the floor tipping > the entire bowl all over onto myself. > > Upon hearing the clamor of me hitting the floor and the metal bowl > banging around a rush of ER staff charged in and I got prompt service > thereafter. I still have the scar, but I never cut myself again :-) > > Brett > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Research > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > PH 215-652-2501 fax. 215-993-6803 > e-mail. brett_connolly@merck.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie > M O'Connor > Sent: Monday, October 27, 2008 4:44 PM > To: Peter Carroll > Cc: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Protect fingers during Microtomy > > OK - time to poll - who has had sutures from microtomy? Me! 23 years > > ago - 8 months pregnant, and not paying attention to what I was doing. > Oh > yeah, and one other time, I cut off the tip of my finger beta testing > a microtome safety invention from EHS. > > > > > > Peter Carroll > Sent by: histonet-bounces@lists.utsouthwestern.edu > 10/27/2008 03:17 PM > > To > > cc > histonet@lists.utsouthwestern.edu > Subject > Re: [Histonet] Protect fingers during Microtomy > > > > > > > > I am looking some think (like gloves)to protect the fingers during > microtomy > > No offense, but if you're that prone to injury, I recommend steering > clear of microtomes in general ;) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates (which may be known > outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD > and in Japan, as Banyu - direct contact information for affiliates is > available at http://www.merck.com/contact/contacts.html) that may be > confidential, proprietary copyrighted and/or legally privileged. It is > intended solely for the use of the individual or entity named on this > message. If you are not the intended recipient, and have received this > message in error, please notify us immediately by reply e-mail and > then delete it from your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please reply to the sender that > you have received the message in error, then delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From JWeems <@t> sjha.org Tue Oct 28 12:11:54 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Oct 28 12:12:28 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1744CF0B3C7@EXC-MBX3.cfs.le.ac.uk> References: <490621C6.60304@umdnj.edu><63EA0607835FBA4689CEA9EA8B4826920173B5C0@usctmx1141.merck.com><5CEA6CACB9ABD74881E453BE9D966F24020BE306@exchange01.tarleton.edu><5D64396A0D4A5346BEBC759022AAEAA50E4089@ITSSSXM01V6.one.ads.che.org><5A2BD13465E061429D6455C8D6B40E3907B8F86843@IBMB7Exchange.digestivespecialists.com> <7722595275A4DD4FA225B92CDBF174A1744CF0B3C7@EXC-MBX3.cfs.le.ac.uk> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA50E411E@ITSSSXM01V6.one.ads.che.org> I doubt we can top this one! Hope everyone turned out well... :>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Tuesday, October 28, 2008 12:55 PM To: 'Blazek, Linda'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy My wife, the best section cutter in the world worked right up to the bitter end of her pregnancy with our third daughter, dropped an old style steel knife and inadvertently gave herself a caesarean, beat that histopeople!!!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: 28 October 2008 13:48 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Besides a few little nicks that we all get, about 10 years ago after having worked in histology for almost 25 years I was finally in a lab with windows! Early one morning as I was sitting there cutting with my back to the window, dawn just breaking. A head popped up outside the window and the tech facing the window screamed just as I was running my thumb over the block. I jumped; the chuck came down and sliced into the pad of my thumb down to the bone. I ran to the sink holding my thumb and ran it under water. It's pretty gross to see the bone in your thumb! Funny thing, there was no pain until the ER doc filled it full of the stuff to numb it. Got lots of stitches and even now it sometimes hurts. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, October 28, 2008 9:28 AM To: Telgenhoff, Dr. Dale; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy I've heard that tale, but didn't know who did it! I had a coworked who cut the pad off his thumb. We went to the ER, where I learned he had put it in the trash. I went back and dug through the paraffin scraps and got it - washed it in saline and Betadine.. The ER dr glued it back on and he has a thumbprint again! When I caught myself on fire, cleaning with solvants too close to the hot plate (the fumes ignited), he was the same guy who yelled, "Get the marshmallows - the supervisor's on fire!". I still get a good laugh about that! J:>) And it's only Tues!! Trick or treat!!! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Telgenhoff, Dr. Dale Sent: Tuesday, October 28, 2008 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Not me, but the guy who trained me. Remember the old knife sharpeners that looked like record players? He was removing the blade (they got pretty oily) and it slipped. His natural reaction was to grab for it, slicing three fingers to the bone. Made for a good cautionary tale... ~~~~~~~~~~~~~~~~~~~~ Dale Telgenhoff, PhD Assistant Professor Tarleton State University Clinical Laboratory Science 1501 Enderly Place Fort Worth, TX 76104 telgenhoff@tarleton.edu 817-926-1101 Ext. 7 ~~~~~~~~~~~~~~~~~~~~ ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Connolly, Brett M Sent: Tue 10/28/2008 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Me - 1982 - working in a S. Florida hospital I sliced open the base of my thumb on the corner of a big steel blade as I was sectioning. Didn't even notice it until I saw the blood dripping off my elbow. I went to the ER and was sitting on a gurney with my hand submerged in a bowl of betadine waiting to be stitched up. After what seemed like a pretty long time, I deftly fell off the gurney onto the floor tipping the entire bowl all over onto myself. Upon hearing the clamor of me hitting the floor and the metal bowl banging around a rush of ER staff charged in and I got prompt service thereafter. I still have the scar, but I never cut myself again :-) Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, October 27, 2008 4:44 PM To: Peter Carroll Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Protect fingers during Microtomy OK - time to poll - who has had sutures from microtomy? Me! 23 years ago - 8 months pregnant, and not paying attention to what I was doing. Oh yeah, and one other time, I cut off the tip of my finger beta testing a microtome safety invention from EHS. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 10/27/2008 03:17 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Protect fingers during Microtomy > I am looking some think (like gloves)to protect the fingers during microtomy No offense, but if you're that prone to injury, I recommend steering clear of microtomes in general ;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From cbarone <@t> NEMOURS.ORG Tue Oct 28 12:16:14 2008 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Tue Oct 28 12:16:23 2008 Subject: [Histonet] PVFD Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE801471840@wlmmsx01.nemours.org> Still looking for anyone who has worked with polyvinyl 'hollow fibers'.... Fibers are implanted ... cells grow within...the little straws are removed for processing and staining, but I have found paraffin unsuitable as an embedding media, to support the fibers, regardless of the fact I have tried every hing from freezing them, to double embedding...must I go to plastic...and if so, which is best...I will need to run immuno's in the future. PS: They cut fin in paraffin in transverse sections...but, do not cut longitudinally...no surprise! From lblazek <@t> digestivespecialists.com Tue Oct 28 12:22:40 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Oct 28 12:21:26 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA50E411B@ITSSSXM01V6.one.ads.che.org> References: <5D64396A0D4A5346BEBC759022AAEAA50E4089@ITSSSXM01V6.one.ads.che.org> <5D64396A0D4A5346BEBC759022AAEAA50E411B@ITSSSXM01V6.one.ads.che.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E3907B8F86851@IBMB7Exchange.digestivespecialists.com> We also stropped our knives. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, October 28, 2008 1:10 PM To: Judith L. Williams Cc: histonet@lists.utsouthwestern.edu; Telgenhoff, Dr. Dale Subject: RE: [Histonet] Protect fingers during Microtomy Barefoot, no less... -----Original Message----- From: Judith L. Williams [mailto:juditw@u.washington.edu] Sent: Tuesday, October 28, 2008 12:27 PM To: Weems, Joyce Cc: Telgenhoff, Dr. Dale; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Hey- Are all you young "in training" histotechs out there listening and learning???:-) yes, and we walked up hill in the snow to work!! (both ways- and sharpened our knives by HAND -eeeewww- how quaint!) Judy in Washington On Tue, 28 Oct 2008, Weems, Joyce wrote: > I've heard that tale, but didn't know who did it! > > I had a coworked who cut the pad off his thumb. We went to the ER, > where I learned he had put it in the trash. I went back and dug > through the paraffin scraps and got it - washed it in saline and > Betadine.. The ER dr glued it back on and he has a thumbprint again! > > When I caught myself on fire, cleaning with solvants too close to the > hot plate (the fumes ignited), he was the same guy who yelled, "Get > the marshmallows - the supervisor's on fire!". I still get a good > laugh about that! J:>) > > And it's only Tues!! Trick or treat!!! > > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > Please note new phone and fax numbers > 678-843-7376 - Phone > 678-843-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Telgenhoff, Dr. Dale > Sent: Tuesday, October 28, 2008 9:18 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Protect fingers during Microtomy > > Not me, but the guy who trained me. Remember the old knife sharpeners > that looked like record players? He was removing the blade (they got > pretty oily) and it slipped. His natural reaction was to grab for it, > slicing three fingers to the bone. Made for a good cautionary tale... > > ~~~~~~~~~~~~~~~~~~~~ > Dale Telgenhoff, PhD > Assistant Professor > Tarleton State University > Clinical Laboratory Science > 1501 Enderly Place > Fort Worth, TX 76104 > telgenhoff@tarleton.edu > 817-926-1101 Ext. 7 > ~~~~~~~~~~~~~~~~~~~~ > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Connolly, > Brett M > Sent: Tue 10/28/2008 8:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Protect fingers during Microtomy > > > > > Me - 1982 - working in a S. Florida hospital I sliced open the base of > my thumb on the corner of a big steel blade as I was sectioning. > Didn't even notice it until I saw the blood dripping off my elbow. > > I went to the ER and was sitting on a gurney with my hand submerged in > a bowl of betadine waiting to be stitched up. After what seemed like a > pretty long time, I deftly fell off the gurney onto the floor tipping > the entire bowl all over onto myself. > > Upon hearing the clamor of me hitting the floor and the metal bowl > banging around a rush of ER staff charged in and I got prompt service > thereafter. I still have the scar, but I never cut myself again :-) > > Brett > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Research > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > PH 215-652-2501 fax. 215-993-6803 > e-mail. brett_connolly@merck.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie > M O'Connor > Sent: Monday, October 27, 2008 4:44 PM > To: Peter Carroll > Cc: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] Protect fingers during Microtomy > > OK - time to poll - who has had sutures from microtomy? Me! 23 years > > ago - 8 months pregnant, and not paying attention to what I was doing. > Oh > yeah, and one other time, I cut off the tip of my finger beta testing > a microtome safety invention from EHS. > > > > > > Peter Carroll > Sent by: histonet-bounces@lists.utsouthwestern.edu > 10/27/2008 03:17 PM > > To > > cc > histonet@lists.utsouthwestern.edu > Subject > Re: [Histonet] Protect fingers during Microtomy > > > > > > > > I am looking some think (like gloves)to protect the fingers during > microtomy > > No offense, but if you're that prone to injury, I recommend steering > clear of microtomes in general ;) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates (which may be known > outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD > and in Japan, as Banyu - direct contact information for affiliates is > available at http://www.merck.com/contact/contacts.html) that may be > confidential, proprietary copyrighted and/or legally privileged. It is > intended solely for the use of the individual or entity named on this > message. If you are not the intended recipient, and have received this > message in error, please notify us immediately by reply e-mail and > then delete it from your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the property of Catholic > Health East and is intended for the sole use of the intended > recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please reply to the sender that > you have received the message in error, then delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gmartin <@t> marshallmedical.org Tue Oct 28 12:23:24 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Tue Oct 28 12:23:29 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1744CF0B3C7@EXC-MBX3.cfs.le.ac.uk> References: <490621C6.60304@umdnj.edu><63EA0607835FBA4689CEA9EA8B4826920173B5C0@usctmx1141.merck.com><5CEA6CACB9ABD74881E453BE9D966F24020BE306@exchange01.tarleton.edu><5D64396A0D4A5346BEBC759022AAEAA50E4089@ITSSSXM01V6.one.ads.che.org><5A2BD13465E061429D6455C8D6B40E3907B8F86843@IBMB7Exchange.digestivespecialists.com> <7722595275A4DD4FA225B92CDBF174A1744CF0B3C7@EXC-MBX3.cfs.le.ac.uk> Message-ID: <6ED9D4252F278841A0593D3D788AF24C039A871A@mailsvr.MARSHMED.local> Do you mean she was "barefoot, pregnant, and in the lab"!! How could you! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Tuesday, October 28, 2008 9:55 AM To: 'Blazek, Linda'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy My wife, the best section cutter in the world worked right up to the bitter end of her pregnancy with our third daughter, dropped an old style steel knife and inadvertently gave herself a caesarean, beat that histopeople!!!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: 28 October 2008 13:48 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Besides a few little nicks that we all get, about 10 years ago after having worked in histology for almost 25 years I was finally in a lab with windows! Early one morning as I was sitting there cutting with my back to the window, dawn just breaking. A head popped up outside the window and the tech facing the window screamed just as I was running my thumb over the block. I jumped; the chuck came down and sliced into the pad of my thumb down to the bone. I ran to the sink holding my thumb and ran it under water. It's pretty gross to see the bone in your thumb! Funny thing, there was no pain until the ER doc filled it full of the stuff to numb it. Got lots of stitches and even now it sometimes hurts. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, October 28, 2008 9:28 AM To: Telgenhoff, Dr. Dale; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy I've heard that tale, but didn't know who did it! I had a coworked who cut the pad off his thumb. We went to the ER, where I learned he had put it in the trash. I went back and dug through the paraffin scraps and got it - washed it in saline and Betadine.. The ER dr glued it back on and he has a thumbprint again! When I caught myself on fire, cleaning with solvants too close to the hot plate (the fumes ignited), he was the same guy who yelled, "Get the marshmallows - the supervisor's on fire!". I still get a good laugh about that! J:>) And it's only Tues!! Trick or treat!!! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Telgenhoff, Dr. Dale Sent: Tuesday, October 28, 2008 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Not me, but the guy who trained me. Remember the old knife sharpeners that looked like record players? He was removing the blade (they got pretty oily) and it slipped. His natural reaction was to grab for it, slicing three fingers to the bone. Made for a good cautionary tale... ~~~~~~~~~~~~~~~~~~~~ Dale Telgenhoff, PhD Assistant Professor Tarleton State University Clinical Laboratory Science 1501 Enderly Place Fort Worth, TX 76104 telgenhoff@tarleton.edu 817-926-1101 Ext. 7 ~~~~~~~~~~~~~~~~~~~~ ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Connolly, Brett M Sent: Tue 10/28/2008 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Me - 1982 - working in a S. Florida hospital I sliced open the base of my thumb on the corner of a big steel blade as I was sectioning. Didn't even notice it until I saw the blood dripping off my elbow. I went to the ER and was sitting on a gurney with my hand submerged in a bowl of betadine waiting to be stitched up. After what seemed like a pretty long time, I deftly fell off the gurney onto the floor tipping the entire bowl all over onto myself. Upon hearing the clamor of me hitting the floor and the metal bowl banging around a rush of ER staff charged in and I got prompt service thereafter. I still have the scar, but I never cut myself again :-) Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, October 27, 2008 4:44 PM To: Peter Carroll Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Protect fingers during Microtomy OK - time to poll - who has had sutures from microtomy? Me! 23 years ago - 8 months pregnant, and not paying attention to what I was doing. Oh yeah, and one other time, I cut off the tip of my finger beta testing a microtome safety invention from EHS. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 10/27/2008 03:17 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Protect fingers during Microtomy > I am looking some think (like gloves)to protect the fingers during microtomy No offense, but if you're that prone to injury, I recommend steering clear of microtomes in general ;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Tue Oct 28 12:29:31 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Oct 28 12:29:48 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C039A871A@mailsvr.MARSHMED.local> References: <490621C6.60304@umdnj.edu><63EA0607835FBA4689CEA9EA8B4826920173B5C0@usctmx1141.merck.com><5CEA6CACB9ABD74881E453BE9D966F24020BE306@exchange01.tarleton.edu><5D64396A0D4A5346BEBC759022AAEAA50E4089@ITSSSXM01V6.one.ads.che.org><5A2BD13465E061429D6455C8D6B40E3907B8F86843@IBMB7Exchange.digestivespecialists.com><7722595275A4DD4FA225B92CDBF174A1744CF0B3C7@EXC-MBX3.cfs.le.ac.uk> <6ED9D4252F278841A0593D3D788AF24C039A871A@mailsvr.MARSHMED.local> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA50E412C@ITSSSXM01V6.one.ads.che.org> No - that was me... East Tennessee you know!!! It was my job to sharpen the knives on the old noisy wheel machine. My first child had fits when I did that. I'm surprised she's not deaf, but seems to have always had good hearing. She was perfectly level with the noise and probably was vibrated till she rattled! Ah... Those good old days... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Tuesday, October 28, 2008 1:23 PM To: Edwards, R.E.; Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Do you mean she was "barefoot, pregnant, and in the lab"!! How could you! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Tuesday, October 28, 2008 9:55 AM To: 'Blazek, Linda'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy My wife, the best section cutter in the world worked right up to the bitter end of her pregnancy with our third daughter, dropped an old style steel knife and inadvertently gave herself a caesarean, beat that histopeople!!!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: 28 October 2008 13:48 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Besides a few little nicks that we all get, about 10 years ago after having worked in histology for almost 25 years I was finally in a lab with windows! Early one morning as I was sitting there cutting with my back to the window, dawn just breaking. A head popped up outside the window and the tech facing the window screamed just as I was running my thumb over the block. I jumped; the chuck came down and sliced into the pad of my thumb down to the bone. I ran to the sink holding my thumb and ran it under water. It's pretty gross to see the bone in your thumb! Funny thing, there was no pain until the ER doc filled it full of the stuff to numb it. Got lots of stitches and even now it sometimes hurts. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, October 28, 2008 9:28 AM To: Telgenhoff, Dr. Dale; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy I've heard that tale, but didn't know who did it! I had a coworked who cut the pad off his thumb. We went to the ER, where I learned he had put it in the trash. I went back and dug through the paraffin scraps and got it - washed it in saline and Betadine.. The ER dr glued it back on and he has a thumbprint again! When I caught myself on fire, cleaning with solvants too close to the hot plate (the fumes ignited), he was the same guy who yelled, "Get the marshmallows - the supervisor's on fire!". I still get a good laugh about that! J:>) And it's only Tues!! Trick or treat!!! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Telgenhoff, Dr. Dale Sent: Tuesday, October 28, 2008 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Not me, but the guy who trained me. Remember the old knife sharpeners that looked like record players? He was removing the blade (they got pretty oily) and it slipped. His natural reaction was to grab for it, slicing three fingers to the bone. Made for a good cautionary tale... ~~~~~~~~~~~~~~~~~~~~ Dale Telgenhoff, PhD Assistant Professor Tarleton State University Clinical Laboratory Science 1501 Enderly Place Fort Worth, TX 76104 telgenhoff@tarleton.edu 817-926-1101 Ext. 7 ~~~~~~~~~~~~~~~~~~~~ ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Connolly, Brett M Sent: Tue 10/28/2008 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Me - 1982 - working in a S. Florida hospital I sliced open the base of my thumb on the corner of a big steel blade as I was sectioning. Didn't even notice it until I saw the blood dripping off my elbow. I went to the ER and was sitting on a gurney with my hand submerged in a bowl of betadine waiting to be stitched up. After what seemed like a pretty long time, I deftly fell off the gurney onto the floor tipping the entire bowl all over onto myself. Upon hearing the clamor of me hitting the floor and the metal bowl banging around a rush of ER staff charged in and I got prompt service thereafter. I still have the scar, but I never cut myself again :-) Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, October 27, 2008 4:44 PM To: Peter Carroll Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Protect fingers during Microtomy OK - time to poll - who has had sutures from microtomy? Me! 23 years ago - 8 months pregnant, and not paying attention to what I was doing. Oh yeah, and one other time, I cut off the tip of my finger beta testing a microtome safety invention from EHS. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 10/27/2008 03:17 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Protect fingers during Microtomy > I am looking some think (like gloves)to protect the fingers during microtomy No offense, but if you're that prone to injury, I recommend steering clear of microtomes in general ;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Phyllis_Burnette <@t> bshsi.org Tue Oct 28 13:09:11 2008 From: Phyllis_Burnette <@t> bshsi.org (Burnette, Phyllis) Date: Tue Oct 28 13:09:18 2008 Subject: [Histonet] Cmv control Message-ID: <2C7750460C3E7545AC11099BE6A7ABD601C9939E@EDC-MAIL-02.ads.bshsi.com> Good day, I need a cytomegalovirus control. Need formalin-fixed paraffin block. Any suggestions? Phyllis Burnette Histology Supervisor BSHR Laboratories 398-4763 680-1008 pager ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From MElliott <@t> mrl.ubc.ca Tue Oct 28 14:07:14 2008 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Tue Oct 28 14:07:42 2008 Subject: [Histonet] Human Type II pneumocytes Message-ID: <49070072.11C6.00D6.0@mrl.ubc.ca> Hi everyone. I was searching the Archives for some information and came across the same question I was looking for but could find no answers to Andrea Hooper's question from 2005. Does anyone know of an antibody or marker specific for human alveolar Type II pneumocytes? Mark In sunny Vancouver BC ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From kdboydhisto <@t> yahoo.com Tue Oct 28 14:36:47 2008 From: kdboydhisto <@t> yahoo.com (KELLY BOYD) Date: Tue Oct 28 14:36:55 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C039A871A@mailsvr.MARSHMED.local> Message-ID: <158337.48241.qm@web58605.mail.re3.yahoo.com> Thanks for the great laughs today! Really needed that! It's not funny when it happens, but great to laugh at later! Been there and done that too. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 Cell? (252)-943-9527 Fax? (252)-830-0032 ? ? --- On Tue, 10/28/08, Martin, Gary wrote: From: Martin, Gary Subject: RE: [Histonet] Protect fingers during Microtomy To: "Edwards, R.E." , "Blazek, Linda" , histonet@lists.utsouthwestern.edu Date: Tuesday, October 28, 2008, 1:23 PM Do you mean she was "barefoot, pregnant, and in the lab"!! How could you! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Tuesday, October 28, 2008 9:55 AM To: 'Blazek, Linda'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy My wife, the best section cutter in the world worked right up to the bitter end of her pregnancy with our third daughter, dropped an old style steel knife and inadvertently gave herself a caesarean, beat that histopeople!!!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: 28 October 2008 13:48 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Besides a few little nicks that we all get, about 10 years ago after having worked in histology for almost 25 years I was finally in a lab with windows! Early one morning as I was sitting there cutting with my back to the window, dawn just breaking. A head popped up outside the window and the tech facing the window screamed just as I was running my thumb over the block. I jumped; the chuck came down and sliced into the pad of my thumb down to the bone. I ran to the sink holding my thumb and ran it under water. It's pretty gross to see the bone in your thumb! Funny thing, there was no pain until the ER doc filled it full of the stuff to numb it. Got lots of stitches and even now it sometimes hurts. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, October 28, 2008 9:28 AM To: Telgenhoff, Dr. Dale; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy I've heard that tale, but didn't know who did it! I had a coworked who cut the pad off his thumb. We went to the ER, where I learned he had put it in the trash. I went back and dug through the paraffin scraps and got it - washed it in saline and Betadine.. The ER dr glued it back on and he has a thumbprint again! When I caught myself on fire, cleaning with solvants too close to the hot plate (the fumes ignited), he was the same guy who yelled, "Get the marshmallows - the supervisor's on fire!". I still get a good laugh about that! J:>) And it's only Tues!! Trick or treat!!! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Telgenhoff, Dr. Dale Sent: Tuesday, October 28, 2008 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Not me, but the guy who trained me. Remember the old knife sharpeners that looked like record players? He was removing the blade (they got pretty oily) and it slipped. His natural reaction was to grab for it, slicing three fingers to the bone. Made for a good cautionary tale... ~~~~~~~~~~~~~~~~~~~~ Dale Telgenhoff, PhD Assistant Professor Tarleton State University Clinical Laboratory Science 1501 Enderly Place Fort Worth, TX 76104 telgenhoff@tarleton.edu 817-926-1101 Ext. 7 ~~~~~~~~~~~~~~~~~~~~ ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Connolly, Brett M Sent: Tue 10/28/2008 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Me - 1982 - working in a S. Florida hospital I sliced open the base of my thumb on the corner of a big steel blade as I was sectioning. Didn't even notice it until I saw the blood dripping off my elbow. I went to the ER and was sitting on a gurney with my hand submerged in a bowl of betadine waiting to be stitched up. After what seemed like a pretty long time, I deftly fell off the gurney onto the floor tipping the entire bowl all over onto myself. Upon hearing the clamor of me hitting the floor and the metal bowl banging around a rush of ER staff charged in and I got prompt service thereafter. I still have the scar, but I never cut myself again :-) Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, October 27, 2008 4:44 PM To: Peter Carroll Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Protect fingers during Microtomy OK - time to poll - who has had sutures from microtomy? Me! 23 years ago - 8 months pregnant, and not paying attention to what I was doing. Oh yeah, and one other time, I cut off the tip of my finger beta testing a microtome safety invention from EHS. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 10/27/2008 03:17 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Protect fingers during Microtomy > I am looking some think (like gloves)to protect the fingers during microtomy No offense, but if you're that prone to injury, I recommend steering clear of microtomes in general ;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Tue Oct 28 14:44:40 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Oct 28 14:46:51 2008 Subject: [Histonet] Protect fingers during Microtomy References: <158337.48241.qm@web58605.mail.re3.yahoo.com> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120196@uwhis-xchng3.uwhis.hosp.wisc.edu> Thanks guys. You help to keep me in line. Whenever I start getting a big head and get cocky, I read about all the stuff you guys used to deal with and realize how spoiled I am and how much I still have to learn. Anybody have a towel for that wet spot behind my ears? Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of KELLY BOYD Sent: Tue 10/28/2008 2:36 PM To: Martin, Gary; histonet Subject: RE: [Histonet] Protect fingers during Microtomy Thanks for the great laughs today! Really needed that! It's not funny when it happens, but great to laugh at later! Been there and done that too. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 --- On Tue, 10/28/08, Martin, Gary wrote: From: Martin, Gary Subject: RE: [Histonet] Protect fingers during Microtomy To: "Edwards, R.E." , "Blazek, Linda" , histonet@lists.utsouthwestern.edu Date: Tuesday, October 28, 2008, 1:23 PM Do you mean she was "barefoot, pregnant, and in the lab"!! How could you! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Tuesday, October 28, 2008 9:55 AM To: 'Blazek, Linda'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy My wife, the best section cutter in the world worked right up to the bitter end of her pregnancy with our third daughter, dropped an old style steel knife and inadvertently gave herself a caesarean, beat that histopeople!!!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: 28 October 2008 13:48 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Besides a few little nicks that we all get, about 10 years ago after having worked in histology for almost 25 years I was finally in a lab with windows! Early one morning as I was sitting there cutting with my back to the window, dawn just breaking. A head popped up outside the window and the tech facing the window screamed just as I was running my thumb over the block. I jumped; the chuck came down and sliced into the pad of my thumb down to the bone. I ran to the sink holding my thumb and ran it under water. It's pretty gross to see the bone in your thumb! Funny thing, there was no pain until the ER doc filled it full of the stuff to numb it. Got lots of stitches and even now it sometimes hurts. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, October 28, 2008 9:28 AM To: Telgenhoff, Dr. Dale; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy I've heard that tale, but didn't know who did it! I had a coworked who cut the pad off his thumb. We went to the ER, where I learned he had put it in the trash. I went back and dug through the paraffin scraps and got it - washed it in saline and Betadine.. The ER dr glued it back on and he has a thumbprint again! When I caught myself on fire, cleaning with solvants too close to the hot plate (the fumes ignited), he was the same guy who yelled, "Get the marshmallows - the supervisor's on fire!". I still get a good laugh about that! J:>) And it's only Tues!! Trick or treat!!! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Telgenhoff, Dr. Dale Sent: Tuesday, October 28, 2008 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Not me, but the guy who trained me. Remember the old knife sharpeners that looked like record players? He was removing the blade (they got pretty oily) and it slipped. His natural reaction was to grab for it, slicing three fingers to the bone. Made for a good cautionary tale... ~~~~~~~~~~~~~~~~~~~~ Dale Telgenhoff, PhD Assistant Professor Tarleton State University Clinical Laboratory Science 1501 Enderly Place Fort Worth, TX 76104 telgenhoff@tarleton.edu 817-926-1101 Ext. 7 ~~~~~~~~~~~~~~~~~~~~ ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Connolly, Brett M Sent: Tue 10/28/2008 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protect fingers during Microtomy Me - 1982 - working in a S. Florida hospital I sliced open the base of my thumb on the corner of a big steel blade as I was sectioning. Didn't even notice it until I saw the blood dripping off my elbow. I went to the ER and was sitting on a gurney with my hand submerged in a bowl of betadine waiting to be stitched up. After what seemed like a pretty long time, I deftly fell off the gurney onto the floor tipping the entire bowl all over onto myself. Upon hearing the clamor of me hitting the floor and the metal bowl banging around a rush of ER staff charged in and I got prompt service thereafter. I still have the scar, but I never cut myself again :-) Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, October 27, 2008 4:44 PM To: Peter Carroll Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Protect fingers during Microtomy OK - time to poll - who has had sutures from microtomy? Me! 23 years ago - 8 months pregnant, and not paying attention to what I was doing. Oh yeah, and one other time, I cut off the tip of my finger beta testing a microtome safety invention from EHS. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 10/27/2008 03:17 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Protect fingers during Microtomy > I am looking some think (like gloves)to protect the fingers during microtomy No offense, but if you're that prone to injury, I recommend steering clear of microtomes in general ;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwich <@t> 7thwavelabs.com Tue Oct 28 14:46:56 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Tue Oct 28 14:47:02 2008 Subject: [Histonet] cryostat SOP Message-ID: <62A8156F8071C8439080D626DF8C33A602E51B@wave-mail.7thwave.local> An SOP question for those out there who are forced to write these tedious things: Do you maintain a separate cryostat SOP, or is it something that is typically included in a general microtome SOP? Any help with this would be greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From mwich <@t> 7thwavelabs.com Tue Oct 28 15:14:27 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Tue Oct 28 15:14:30 2008 Subject: [Histonet] cryostat SOP response Message-ID: <62A8156F8071C8439080D626DF8C33A602E51E@wave-mail.7thwave.local> Thanks so much for the prompt feedback on this! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From tpodawiltz <@t> lrgh.org Tue Oct 28 16:15:05 2008 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Oct 28 16:16:02 2008 Subject: [Histonet] RE: cryostat SOP In-Reply-To: <62A8156F8071C8439080D626DF8C33A602E51B@wave-mail.7thwave.local> References: <62A8156F8071C8439080D626DF8C33A602E51B@wave-mail.7thwave.local> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D2D14DB33@LRGHEXVS1.practice.lrgh.org> I just have the cryostat as a procedure in the main Histology Procedure Manual. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich [mwich@7thwavelabs.com] Sent: Tuesday, October 28, 2008 3:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat SOP An SOP question for those out there who are forced to write these tedious things: Do you maintain a separate cryostat SOP, or is it something that is typically included in a general microtome SOP? Any help with this would be greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From lchen <@t> mednet.ucla.edu Tue Oct 28 16:40:07 2008 From: lchen <@t> mednet.ucla.edu (Leslie Chen) Date: Tue Oct 28 16:40:30 2008 Subject: [Histonet] Protect fingers during Microtomy Message-ID: <001c01c93945$c7ca2450$3ba62f0a@DHGLABC99E93DF> They make cut resistant gloves for kitchen staff in restaurants to keep workers from cutting their hands. Don't know if this would work for microtomy as I think it would get in the way of sectioning..... . Leslie ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. From JWeems <@t> sjha.org Tue Oct 28 16:58:05 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Oct 28 16:58:13 2008 Subject: [Histonet] RE: cryostat SOP In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638631D2D14DB33@LRGHEXVS1.practice.lrgh.org> References: <62A8156F8071C8439080D626DF8C33A602E51B@wave-mail.7thwave.local> <38667E7FB77ECD4E91BFAEB8D98638631D2D14DB33@LRGHEXVS1.practice.lrgh.org> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA50E41AE@ITSSSXM01V6.one.ads.che.org> We do too. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Tuesday, October 28, 2008 5:15 PM To: Michele Wich; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: cryostat SOP I just have the cryostat as a procedure in the main Histology Procedure Manual. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich [mwich@7thwavelabs.com] Sent: Tuesday, October 28, 2008 3:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat SOP An SOP question for those out there who are forced to write these tedious things: Do you maintain a separate cryostat SOP, or is it something that is typically included in a general microtome SOP? Any help with this would be greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Robert.Lott <@t> TriadHospitals.com Tue Oct 28 17:24:46 2008 From: Robert.Lott <@t> TriadHospitals.com (Lott, Robert) Date: Tue Oct 28 17:25:15 2008 Subject: [Histonet] Human Type II pneumocytes Message-ID: <4A3619571D9F6C4CB79C980E91DBE4E67F609D@TNTRIEXEVS03.triadhospitals.net> Mark, I not 100% sure, but I believe that Napsin A antibodies will stain type II pneumocytes. Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Trinity Medical Center/ LabFirst 800 Montclair Road Birmingham, AL 35213-1984 robert.lott@triadhospitals.com 205-592-5387 (office) 205-592-5388 (lab) 205-592-5646 (fax) Message: 8 Date: Tue, 28 Oct 2008 12:07:14 -0700 From: "Mark Elliott" Subject: [Histonet] Human Type II pneumocytes To: , Message-ID: <49070072.11C6.00D6.0@mrl.ubc.ca> Content-Type: text/plain; charset=US-ASCII Hi everyone. I was searching the Archives for some information and came across the same question I was looking for but could find no answers to Andrea Hooper's question from 2005. Does anyone know of an antibody or marker specific for human alveolar Type II pneumocytes? Mark In sunny Vancouver BC -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From ajaivyas <@t> gmail.com Tue Oct 28 19:33:51 2008 From: ajaivyas <@t> gmail.com (Ajai Vyas) Date: Tue Oct 28 19:33:58 2008 Subject: [Histonet] Re: Histonet Digest, Vol 59, Issue 35 In-Reply-To: <49071022.050bca0a.6d66.283fSMTPIN_ADDED@mx.google.com> References: <49071022.050bca0a.6d66.283fSMTPIN_ADDED@mx.google.com> Message-ID: Hi, How long PFA perfused brains will remain good for IHC if stored at -70? And how long flash frozen brains will remain suitable for ISH if stored at -70? Is there any way to increase storage life? Any comment will be greatly appreciated. Ajai From arvidsonkristen <@t> yahoo.com Wed Oct 29 03:49:51 2008 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Wed Oct 29 03:49:56 2008 Subject: [Histonet] QA/QC Message-ID: <687967.37200.qm@web65703.mail.ac4.yahoo.com> I am looking to update our QA/QC system...it's a mess.? Any suggestions on where I would gather info.? What are other people doing?? From DELONG_CYNTHIA_A <@t> LILLY.COM Wed Oct 29 07:26:47 2008 From: DELONG_CYNTHIA_A <@t> LILLY.COM (Cynthia A Delong) Date: Wed Oct 29 07:26:55 2008 Subject: [Histonet] Acidic mounting media Message-ID: I have a researcher who is trying to duplicate a Fluoro-Jade C stain and they mount in a acidic mounting media(pH 4.5) It is a 0.1% acetic acid and 80% glycerin. Does anyone have a protocol for this? Cynthia A DeLong From rjbuesa <@t> yahoo.com Wed Oct 29 07:58:53 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 29 07:58:57 2008 Subject: [Histonet] cryostat SOP In-Reply-To: <62A8156F8071C8439080D626DF8C33A602E51B@wave-mail.7thwave.local> Message-ID: <655602.8333.qm@web65709.mail.ac4.yahoo.com> As part of the general SOP, under sectioning. Ren? J. --- On Tue, 10/28/08, Michele Wich wrote: From: Michele Wich Subject: [Histonet] cryostat SOP To: histonet@lists.utsouthwestern.edu Date: Tuesday, October 28, 2008, 3:46 PM An SOP question for those out there who are forced to write these tedious things: Do you maintain a separate cryostat SOP, or is it something that is typically included in a general microtome SOP? Any help with this would be greatly appreciated! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Wed Oct 29 10:04:34 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Wed Oct 29 10:05:19 2008 Subject: [Histonet] QA/QC In-Reply-To: <687967.37200.qm@web65703.mail.ac4.yahoo.com> Message-ID: <04DD060342EA4A469578751974DFE207@wchsys.org> Check the NSH web site they offer QC/QA material in their information available to members. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Wednesday, October 29, 2008 4:50 AM To: histonet Subject: [Histonet] QA/QC I am looking to update our QA/QC system...it's a mess.? Any suggestions on where I would gather info.? What are other people doing?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From shive003 <@t> umn.edu Wed Oct 29 10:18:24 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Oct 29 10:18:32 2008 Subject: [Histonet] QA/QC References: <687967.37200.qm@web65703.mail.ac4.yahoo.com> Message-ID: We instituted an online system called QPulse. It tracks procedure SOPs, equipment calibration, training records, etc. You might check that out. Jan Shivers IHC/Histo/EM Section Head UMN Veterinary Diagnostic Lab St. Paul, MN ----- Original Message ----- From: "kristen arvidson" To: "histonet" Sent: Wednesday, October 29, 2008 3:49 AM Subject: [Histonet] QA/QC I am looking to update our QA/QC system...it's a mess. Any suggestions on where I would gather info. What are other people doing?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Wed Oct 29 11:01:03 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Oct 29 11:01:08 2008 Subject: [Histonet] turkey eggs Message-ID: <1D458DA677884330BC705491BDBAFDD4@auxs.umn.edu> Has anyone ever fixed and serial sectioned whole (turkey) eggs, including shell? I'm looking for a lab that might be able to accommodate such things. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From ree3 <@t> leicester.ac.uk Wed Oct 29 11:12:25 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed Oct 29 11:12:31 2008 Subject: [Histonet] turkey eggs In-Reply-To: <1D458DA677884330BC705491BDBAFDD4@auxs.umn.edu> References: <1D458DA677884330BC705491BDBAFDD4@auxs.umn.edu> Message-ID: <7722595275A4DD4FA225B92CDBF174A1744CF0B3D3@EXC-MBX3.cfs.le.ac.uk> You must be yolking!!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: 29 October 2008 16:01 To: histonet Subject: [Histonet] turkey eggs Has anyone ever fixed and serial sectioned whole (turkey) eggs, including shell? I'm looking for a lab that might be able to accommodate such things. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rnewlin <@t> burnham.org Wed Oct 29 12:37:16 2008 From: rnewlin <@t> burnham.org (Robbin Newlin) Date: Wed Oct 29 12:40:53 2008 Subject: [Histonet] Protect fingers during Microtomy In-Reply-To: <001c01c93945$c7ca2450$3ba62f0a@DHGLABC99E93DF> References: <001c01c93945$c7ca2450$3ba62f0a@DHGLABC99E93DF> Message-ID: <1EA4D719897F2E41B05237767D806EB6026E5EF354@MAIL07.burnham.org> We ordered cut resistant gloves from surgipath and they are great, do not interfere with sectioning at all I run a core facility so we have lots of newbies and they have reduced our injuries significantly. Robbin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leslie Chen Sent: Tuesday, October 28, 2008 2:40 PM To: carrolpb@umdnj.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Protect fingers during Microtomy They make cut resistant gloves for kitchen staff in restaurants to keep workers from cutting their hands. Don't know if this would work for microtomy as I think it would get in the way of sectioning..... . Leslie ________________________________ IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hej01 <@t> health.state.ny.us Wed Oct 29 12:44:43 2008 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Wed Oct 29 12:44:53 2008 Subject: [Histonet] rat vaginal smears Message-ID: <6050_1225302288_m9THimqQ023909_OF1D27CC83.6E1C8B60-ON852574F1.00611A32-852574F1.00617A9B@notes.health.state.ny.us> Does anyone have a protocol for staining rat vaginal smears with H&E? Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From kynemitz <@t> travmax.com Wed Oct 29 14:10:54 2008 From: kynemitz <@t> travmax.com (Kyla Nemitz) Date: Wed Oct 29 14:11:33 2008 Subject: [Histonet] Openings update! Message-ID: <9416D9FA37C1C04FA83D69684E95D2E71E4DE85E@exbk2.maxhealth.com> Happy Wednesday Histonet! Can you believe October is almost over? The holidays are coming up soon and if you are thinking you want to make a career change before they arrive (or even early next year), I am here to help! I currently have a couple great positions looking to interview techs ASAP and make offers now or for January starts! Here are my HOT positions this week: Grossing Supervisor - Austin, TX (55-60K+) Histo Tech - San Antonio, TX (47-53K+) Histo Tech - Maryland (40-58K+) All positions above offer competitive/negotiable salary, sign on bonuses and possible relocation. Please feel free to give me a call with any questions, concerns or for additional information on these positions or any others I currently have available. Thank you, hope to hear from you soon! Kyla Nemitz TravelMax Medical Professionals - Maxim Healthcare Tel: 888.800.1855 or 813.371.5175 Fax: 800.294.1248 Search our jobs or apply online at: www.TravelMaxAllied.com From Jenee.S.Odani <@t> hawaii.gov Wed Oct 29 14:29:42 2008 From: Jenee.S.Odani <@t> hawaii.gov (Jenee.S.Odani@hawaii.gov) Date: Wed Oct 29 14:29:46 2008 Subject: Fw: [Histonet] fixative for brains? Message-ID: To everyone who emailed me regarding fixative for mammalian/avian brains: Thank you very much! Based on the feedback I received, I now feel very confident in changing our protocol, and can make a much stronger case to the "powers that be". -J Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 From KLAPANO1 <@t> hfhs.org Wed Oct 29 15:03:05 2008 From: KLAPANO1 <@t> hfhs.org (Karen Lapanowski) Date: Wed Oct 29 15:03:31 2008 Subject: [Histonet] HMGB-1 Message-ID: <49088939020000280000FD88@gwia2v.net.hfh.edu> Hello, Looking for a vendor and a protocol for HMGB-1 in paraffin section from mouse lungs. Any help? Thanks, Karen Lapanowski Henry Ford Health System Radiation Oncology 3065 E & R 313-916-9386 ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. 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If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From JGREWE <@t> OhioHealth.com Wed Oct 29 15:02:34 2008 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Wed Oct 29 15:09:43 2008 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 10/29/2008 and will not return until 11/12/2008. From carrolpb <@t> umdnj.edu Wed Oct 29 15:13:49 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Wed Oct 29 15:14:18 2008 Subject: [Histonet] turkey eggs In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1744CF0B3D3@EXC-MBX3.cfs.le.ac.uk> References: <1D458DA677884330BC705491BDBAFDD4@auxs.umn.edu> <7722595275A4DD4FA225B92CDBF174A1744CF0B3D3@EXC-MBX3.cfs.le.ac.uk> Message-ID: <4908C3FD.3070808@umdnj.edu> > Has anyone ever fixed and serial sectioned whole (turkey) eggs, including shell? an undecalcified shell? how would you fix/process it? From anitaibsc <@t> aol.com Wed Oct 29 15:51:54 2008 From: anitaibsc <@t> aol.com (anitaibsc@aol.com) Date: Wed Oct 29 15:52:00 2008 Subject: [Histonet] Antibody Testing Message-ID: <8CB081F4F54948E-360-1824@mblk-d11.sysops.aol.com> Hello Nettres, Can anyone recommend a lab, that does Immunoflorescent testing for following antibodies: 1. Sialosyl-Tn? antibody, mouse anti human, Tissue. GI tumor, colon Ca will be fine, fresh frozen tissue, alcohol or acetone fixed. ??? We will supply FITC- labeled anti-sialosyl-Tn antibody. We need Immunofluorescence (IF) data. 2. Thyroglobulin (Tg) mouse anti-human, tissue Human thyroid or ca tissue, fresh frozen tissue, alcohol or acetone fixed. ??? We will supply FITC labeled anti-thyroglobulin antibody, we also need IF data. 3. Epithelial specific antigen (ESA), Tissue frozen alcohol or acetone fixed human breast cancer tissue ?? We will supply FITC labeled ant ESA 4. Anti-Leuteinzing hormone (LH), rabbit anti-human, tissue FFPE anterior Pituitary. ??? we will supply rabbit anti-LH. Please let me know if they can do these expt. for us and supply us digital photos. Thank you. Best Regards, Bader Siddiki, PhD ImmunoBioScience Corp. (IBSC) 650-343-IBSC (4272) Email: anitaIBSC@aol.com www.ImmunoBioScience.com From dellav <@t> musc.edu Wed Oct 29 16:07:50 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Wed Oct 29 16:07:55 2008 Subject: [Histonet] rat vaginal smears In-Reply-To: <6050_1225302288_m9THimqQ023909_OF1D27CC83.6E1C8B60-ON852574F1.00611A32-852574F1.00617A9B@notes.health.state.ny.us> References: <6050_1225302288_m9THimqQ023909_OF1D27CC83.6E1C8B60-ON852574F1.00611A32-852574F1.00617A9B@notes.health.state.ny.us> Message-ID: You didn't indicate what you will be evaluating but your request is reminiscent of the work George Papanicolau did with guinea pigs. Have you considered the pap stain? Here is a link that will provide a lot of information if this stain is of interest http://www.statlab.com/Protocol/CytologyStaining.htm I like to think of the pap stain as a 'suped up' H&E Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen E Johnson Sent: Wednesday, October 29, 2008 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] rat vaginal smears Does anyone have a protocol for staining rat vaginal smears with H&E? Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Wed Oct 29 16:10:29 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Oct 29 16:10:36 2008 Subject: [Histonet] [Fwd: qPCR Seminars at Univ of WA & FHCRC] Message-ID: <4908D145.8070508@pathology.washington.edu> For those of you in the Pacific NW. Victor -------- Original Message -------- Subject: qPCR Seminars at Univ of WA & FHCRC Date: Wed, 29 Oct 2008 16:36:52 -0400 (EDT) From: Sigma-Aldrich To: vtobias@u.washington.edu *qPCR Principles and Applications Seminar* *TITLE:* Assay Optimization Techniques to Improve Target Quantification *TWO seminar times are available for your convenience:* *DATE:* Tuesday, November 4, 2008 *TIME:* 10:30 a.m. - 12 noon *PLACE:* Foege Auditorium (UW Genome Sciences, Rm. S-060) * OR * *DATE:* Wednesday, November 5, 2008 *TIME:* 10:00 am - 11:30 a.m. *PLACE:* Pelton Auditorium, FHCRC */Refreshments will be served./* *_Topics that will be discussed include:_* * Improving Assay design * Potential issues with template variability * Improving data normalization * Consistency in data analysis Quantitative PCR addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, diagnostics and other areas, and has become the method of choice for the quantification of nucleic acid targets and identification of sequence-specific variations. Often described as a "gold" standard, these are far from being routine assays. Dr. Nolan is an internationally recognized molecular biologist with an expertise in the field of mRNA quantification using qPCR. She is co-author of the specialist textbook The A-Z of Quantitative PCR (ed S.A. Bustin; IUL Press) and co-author of the SPUD and mRNA integrity assays Sigma Life Science Logo ______________________________________________________ *To continue receiving our emails, add mailuser@sigma-aldrich.com to your address book.* * Follow this link for instructions. * Sigma-Aldrich is committed to never sending unwelcome or unsolicited email. Based on the profile you provided, we believe that you may find this message valuable and informative. If not, please update your profile. Opt-out of future emails from Sigma-Aldrich. Sigma-Aldrich Corp., 3050 Spruce St., St. Louis, MO 63103 USA -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From Vickroy.Jim <@t> mhsil.com Wed Oct 29 17:17:06 2008 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Oct 29 17:17:11 2008 Subject: [Histonet] LOOKING FOR USED CRYOSTAT Message-ID: <24A4826E8EF0964D86BC5317306F58A52BA2A07DC7@mmc-mail.ad.mhsil.com> ANY SUGGESTIONS TO WHERE WE CAN LOCATE A USED CRYOSTAT? Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy.jim@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From saby_joseph_a <@t> yahoo.com Wed Oct 29 17:19:37 2008 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Wed Oct 29 17:19:40 2008 Subject: [Histonet] rat vaginal smears Message-ID: <772278.77886.qm@web33801.mail.mud.yahoo.com> While cutting myh teeth in research many years ago, I performed rat vaginal smears and did indeed use the PAP stain.? It worked very nicely. ________________________________ From: "Della Speranza, Vinnie" To: Helen E Johnson ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, October 29, 2008 5:07:50 PM Subject: RE: [Histonet] rat vaginal smears You didn't indicate what you will be evaluating but your request is reminiscent of the work George Papanicolau did with guinea pigs. Have you considered the pap stain? Here is a link that will provide a lot of information if this stain is of interest http://www.statlab.com/Protocol/CytologyStaining.htm I like to think of the pap stain as a 'suped up' H&E Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue? Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen E Johnson Sent: Wednesday, October 29, 2008 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] rat vaginal smears Does anyone have a protocol for staining rat vaginal smears with H&E? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Helen Johnson? (hej01@health.state.ny.us) IMPORTANT NOTICE:? This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure.? It is intended only for the addressee.? If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments.? Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Wed Oct 29 17:27:44 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Oct 29 17:27:49 2008 Subject: [Histonet] PSLIM Update Message-ID: <4908E360.9060500@pathology.washington.edu> We got our new PSLIM setup in a test environment. First thing we discovered was that all slides are not created equal. We had a box of slides labeled 1" by 3" and also 25mm by 75mm. These slides would not work in the unit. Got another brand labeled 25mm by 75mm and they were just a fraction shorter and worked great. The speed is decent, but not as fast as paper labels. I think your workflow would be to scan the cassette first to start the printing process and then face in the block. Since it usually goes back onto ice to cool, speed shouldn't be an issue. The one feature I like is that it prints the slides in order. #1 comes off first and then it slides #2 under #1 and #3 under #2. The slide is very legible and we have the accession no, block no, cut date, stain name, 2 lines for the facility and a barcode. We have no affiliation with Accuplace. Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From azdudley <@t> hotmail.com Wed Oct 29 21:38:12 2008 From: azdudley <@t> hotmail.com (anita dudley) Date: Wed Oct 29 21:38:18 2008 Subject: [Histonet] IHC QA Message-ID: anyone out there that has a ventana benchmark XT, how do you handle the reports for the pathologist to sign off on when you have more than one path? do you just print one for each dr. or do then pass it around and sigh off on their case? we had a joint com. person tell us that we could not have an empty space? we now send one to each dr., they sigh their cases and we file all the sheets. there are enpty spaces where one signs and the other has signed in another place. what are others doing? thanks for the help. we could have them pass the report around but I know that won't work very well. thanks again, everyone have a good halloween!!!! anita dudley providence hosp mobile alabama _________________________________________________________________ Want to read Hotmail messages in Outlook? The Wordsmiths show you how. http://windowslive.com/connect/post/wedowindowslive.spaces.live.com-Blog-cns!20EE04FBC541789!167.entry?ocid=TXT_TAGLM_WL_hotmail_092008 From WWmn916 <@t> aol.com Wed Oct 29 21:57:39 2008 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Wed Oct 29 21:57:51 2008 Subject: [Histonet] artifact pictures Message-ID: Hi everyone, Question....is the website for posting artifact pictures still up and running? I emailed a few last week and do not see them yet. Is there a different website to post histology pictures for discussion? Thanks, Deb King **************Plan your next getaway with AOL Travel. Check out Today's Hot 5 Travel Deals! (http://travel.aol.com/discount-travel?ncid=emlcntustrav00000001) From laurie <@t> conxis.com Thu Oct 30 05:47:24 2008 From: laurie <@t> conxis.com (Laurie Popp) Date: Thu Oct 30 05:47:31 2008 Subject: [Histonet] Re: Protecting fingers during microtomy Message-ID: <490990BC.6030205@conxis.com> Me...while in training on a clean blade because the supervisor walked up behind me and startled me while I was putting a clean one on after cutting a chunk of bone... little cut and only an incident report. I was told when I entered that lab that you aren't a real histotech until you have cut yourself at least once? The students coming out of our histo school use surgipath's cut resistant gloves but they are only cut resistant... not completely cut proof because when EHS tried to force us all to use them one of my colleagues cut herself pretty good while using them Laurie Popp, BA HT (ASCP) MCR From funderwood <@t> mcohio.org Thu Oct 30 08:16:50 2008 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Thu Oct 30 08:17:01 2008 Subject: [Histonet] infiltrating paraffin disposal Message-ID: <49097AF2.BE64.0034.0@mcohio.org> Hi All, Is it appropriate to dispose of paraffin used in processing by simply sending it out with the day's trash? Will the xylene contained in the solidified block leach out? Thanks, Fred From Susan.Weber2 <@t> va.gov Thu Oct 30 08:20:51 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Thu Oct 30 08:20:59 2008 Subject: [Histonet] IHC QA In-Reply-To: References: Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76E75@VHAV10MSGA1.v10.med.va.gov> I give each Pathologist a report for the batch and eliminate (X off) the cases done by the other Pathologist(s) in reporting area. Yes, more paper is used, but this eliminates "blank" areas and satisfies the requirement. I also request that they indicate if the case would work as potential control tissue and review the sheets as I put them in a binder. This also is a means of making sure that they A) return it, and B) filled them out. I am fortunate that my Pathologists are prompt and concise with their reporting. How are you doing your QC for your H&E stains? I have a similar sheet for them to fill out as well. Each has there own and only fills out the days they read (they rotate the duties). When the sheets are returned at the end of the month, I indicate on the sheet that "*blank lines indicated slides read by other Pathologist". I also check to see that between them, each day is accounted for the month. Hope this helps! Happy Halloween! Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Wednesday, October 29, 2008 10:38 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC QA anyone out there that has a ventana benchmark XT, how do you handle the reports for the pathologist to sign off on when you have more than one path? do you just print one for each dr. or do then pass it around and sigh off on their case? we had a joint com. person tell us that we could not have an empty space? we now send one to each dr., they sigh their cases and we file all the sheets. there are enpty spaces where one signs and the other has signed in another place. what are others doing? thanks for the help. we could have them pass the report around but I know that won't work very well. thanks again, everyone have a good halloween!!!! anita dudley providence hosp mobile alabama _________________________________________________________________ Want to read Hotmail messages in Outlook? The Wordsmiths show you how. http://windowslive.com/connect/post/wedowindowslive.spaces.live.com-Blog -cns!20EE04FBC541789!167.entry?ocid=TXT_TAGLM_WL_hotmail_092008_________ ______________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Oct 30 10:00:29 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Oct 30 10:00:28 2008 Subject: [Histonet] Reply to Ian Montgomery on Cleaning bones Message-ID: Ian Montgomery wrote: I have some bones, from various species, that I want to clean of muscle, tendons, etc, etc. The method currently used is boiling the bones in water for several hours, days until they are completely clean. Problem, it's a wee bit smelly, in fact a big bit smelly. Me being a delicate soul more used to various exotic eau de parfum wonder if there is another technique available. Some species respond to soaking for several weeks in laboratory detergent while others don't. NaOH or KOH, again some do others don't. What I would ideally like is a universal method that's reasonably quick, but not smelly, can anyone help.Reply: Ian, A non smelly way to clean bones is to use an enzyme bleach detergent method. the flesh and also the cartilage will come off the bones without problems. If the bones fall apart, which they tend to do as the enzyme works on all collagen/soft tissue attachments, you will have to keep track of parts, rinse them well and do some gluing. The latter is an anatomy lesson in itself as bone fits together like an interdigitating jigsaw puzzle. I have done bones in unfixed and NBF fixed states, but found fixed bones tend to stay together a bit better. You can do either. i wrapped the bone in cheesecloth and suspended it into the detergent solution when heating it (80F), do not BOIL! The enzyme soap I use in the USA is BIZ detergent and I am not sure you can get this in UK. However you may find another that works. The scent of BIZ keeps you from falling over from nasty smell of boiled bones. You can rinse the bone as you go along, so you know if the soft tissue parts are sliding off bone with ease. I rinsed the bones at completion of cleaning for several hours, then air dried them. BIZ will also bleach the bones in the process. I have some superb museum mounts of bovine skulls, femurs, tibias, etc also sheep, rat and mouse bones. I found a publication back in the 80's in Journal of Anatomy on how to do this on human skulls for museum aka teaching purposes. Good luck on finding the correct soapGayle Callis HT/HTL/MT(ASCP) From gayle.callis <@t> bresnan.net Thu Oct 30 10:13:19 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Oct 30 10:13:18 2008 Subject: [Histonet] ATTN: Louise Renton - reply on Sterchi Eurell Toluidine blue stain for calcified bone Message-ID: <059CEB43A3CF44119C20D3D5523111B5@DHXTS541> Louise, If you have not already received the protocol for the Sterchi/Eurell Toluidine blue and in combination with MacNeals tetrachrome, contact me and I will attach procedures privately. I have been on vacation and found your query in archives. Gayle Callis HT/HTL/MT(ASCP) From mwich <@t> 7thwavelabs.com Thu Oct 30 10:22:39 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Thu Oct 30 10:22:44 2008 Subject: [Histonet] cryostat SOP/fibrin and plasmin antibodies Message-ID: <62A8156F8071C8439080D626DF8C33A602E524@wave-mail.7thwave.local> Thanks again for all responses to my cryostat SOP question. I guess it's time now to write the dang thing. While I'm on here, can anyone recommend a vendor for fibrin and plasmin (plasminogen) antibodies that are reactive in mouse tissue and possibly share a working dilution for either of these? Thanks for any help! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From robinsoc <@t> mercyhealth.com Thu Oct 30 10:43:30 2008 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Thu Oct 30 10:43:49 2008 Subject: [Histonet] bone marrow biopsies Message-ID: <49098FD2.59AC.00AF.0@mercyhealth.com> We are currently using 10% formalin fixation on our bone marrow cores. We fix for 2 hours minimum prior to decal. We are using Immunocal from Decal Corp. for 2-4 hrs followed with processing overnight in VIP. Cores are still crunchy upon sectioning and we are doing surface decal for up to 30 min. Our paths want cores turned out within 24 hrs following procedure. Any suggestions? Thanks. Cindi From lblazek <@t> digestivespecialists.com Thu Oct 30 11:18:15 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Oct 30 11:17:03 2008 Subject: [Histonet] bone marrow biopsies In-Reply-To: <49098FD2.59AC.00AF.0@mercyhealth.com> References: <49098FD2.59AC.00AF.0@mercyhealth.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E3907B8F8686E@IBMB7Exchange.digestivespecialists.com> Schedule all bone marrow biopsies for 6 AM. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Thursday, October 30, 2008 11:44 AM To: histonet Subject: [Histonet] bone marrow biopsies We are currently using 10% formalin fixation on our bone marrow cores. We fix for 2 hours minimum prior to decal. We are using Immunocal from Decal Corp. for 2-4 hrs followed with processing overnight in VIP. Cores are still crunchy upon sectioning and we are doing surface decal for up to 30 min. Our paths want cores turned out within 24 hrs following procedure. Any suggestions? Thanks. Cindi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wa7buxx <@t> yahoo.com Thu Oct 30 13:17:56 2008 From: wa7buxx <@t> yahoo.com (richard wells) Date: Thu Oct 30 13:18:08 2008 Subject: [Histonet] IHC equipment Message-ID: <939640.22785.qm@web36302.mail.mud.yahoo.com> I am helping to set up an in office lab for a group of urologists.? What is the best instrumentation for prostate tripple stain?? What are the best reagents? ? Test volume will be relatively low.? Perhaps as many as 5 additional immunostains will be added in the future.? Testing will be done by an experienced histotech with minimal exposure to immunoperoxidase techniques. ? Goals are to maximize simplicity, dependability and value. ? Thanks in advance for any and all advice. From akbitting <@t> geisinger.edu Thu Oct 30 13:30:19 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Oct 30 13:30:39 2008 Subject: [Histonet] progressive H&E staining using Gills Message-ID: <4909C4FB.2B7F.00C9.0@geisinger.edu> We just switched our H&E stain to a progressive stain using Gill II hematoxylin. Our derm guys love it, but now our GI docs are screaming bloody murder. Does anyone have suggestions for a protocol that will make both 'relatively happy'?? Thanks for letting me pick your brains again!!! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From RSRICHMOND <@t> aol.com Thu Oct 30 14:03:44 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Oct 30 14:03:47 2008 Subject: [Histonet] Re: bone marrow biopsies Message-ID: Cindi Robinson asks: >>We are currently using 10% formalin fixation on our bone marrow cores. We fix for 2 hours minimum prior to decal. We are using Immunocal from Decal Corp. for 2-4 hrs followed with processing overnight in VIP. Cores are still crunchy upon sectioning and we are doing surface decal for up to 30 min. Our paths want cores turned out within 24 hrs following procedure. Any suggestions?<< An ordinary decalcifier (such as Decal Corp's Decal, or several others - just don't use nitric acid) should suffice, and will decalcify marrow cores in an hour or two. Bone marrow specimens obtained after 2 PM should be processed the following day. Would your pathologists and oncologists consent to this compromise? - This is a matter on which, in my experience, pathologists and oncologists don't communicate with each other very well. Bob Richmond Samurai Pathologist Knoxville TN In "commUnIcate", the "U" comes before the "I". From RSRICHMOND <@t> aol.com Thu Oct 30 14:09:16 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Oct 30 14:09:21 2008 Subject: [Histonet] Re: rat vaginal smears Message-ID: Helen Johnson asks: >>Does anyone have a protocol for staining rat vaginal smears with H&E?<< Any H & E suitable for frozen sections should suffice. But it's the wrong stain, assuming you're looking for cornification (keratinization) of superficial squamous cells. I don't know what stain was used traditionally, but if I were to suggest something, it would be the ordinary Pap (Papanicolaou) stain, which stains nuclei with hematoxylin, intermediate cells with light green or fast green, and superficial cells with orange G. - If you buy a Pap stain commercially, get one in which the EA50 and the orange G are separate stains, and not combined. Bob Richmond Samurai Pathologist Knoxville TN From mickie25 <@t> netzero.net Thu Oct 30 14:16:31 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Thu Oct 30 14:16:46 2008 Subject: [Histonet] progressive H&E staining using Gills In-Reply-To: <4909C4FB.2B7F.00C9.0@geisinger.edu> References: <4909C4FB.2B7F.00C9.0@geisinger.edu> Message-ID: Angela, Were you using Harris before? If so, the difference is that Gill formulations stain the mucin of goblet cells quite a dark blue and many GI docs don't like that look. They can get used to it though as ours did at Sacred Heart Medical Center. Hope this helps. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, October 30, 2008 11:30 AM To: histonet Subject: [Histonet] progressive H&E staining using Gills We just switched our H&E stain to a progressive stain using Gill II hematoxylin. Our derm guys love it, but now our GI docs are screaming bloody murder. Does anyone have suggestions for a protocol that will make both 'relatively happy'?? Thanks for letting me pick your brains again!!! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.8.5/1756 - Release Date: 10/30/2008 2:35 PM From Jackie.O'Connor <@t> abbott.com Thu Oct 30 14:36:25 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Oct 30 14:36:51 2008 Subject: [Histonet] progressive H&E staining using Gills In-Reply-To: Message-ID: Richard Allen 7211 Hematoxylin doesn't stain mucin blue, and is a beautiful all around nuclear stain. I'm sure they'll send you a sample. "Mickie Johnson" Sent by: histonet-bounces@lists.utsouthwestern.edu 10/30/2008 02:16 PM Please respond to mickie25@netzero.net To "'Angela Bitting'" , cc Subject RE: [Histonet] progressive H&E staining using Gills Angela, Were you using Harris before? If so, the difference is that Gill formulations stain the mucin of goblet cells quite a dark blue and many GI docs don't like that look. They can get used to it though as ours did at Sacred Heart Medical Center. Hope this helps. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, October 30, 2008 11:30 AM To: histonet Subject: [Histonet] progressive H&E staining using Gills We just switched our H&E stain to a progressive stain using Gill II hematoxylin. Our derm guys love it, but now our GI docs are screaming bloody murder. Does anyone have suggestions for a protocol that will make both 'relatively happy'?? Thanks for letting me pick your brains again!!! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.8.5/1756 - Release Date: 10/30/2008 2:35 PM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SAllen <@t> exchange.hsc.mb.ca Thu Oct 30 15:09:40 2008 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Thu Oct 30 15:10:42 2008 Subject: [Histonet] Modified SDH Message-ID: Hi Histonet, I would like to hear from anyone who does the Modified SDH method. This is the method that uses Phenazine Methosulphate to suppress the normal mitochondria & stains only mitochondria with DNA mutations. We had been doing the test for about 5 years with good results, then we made up new solution & can't seem to get it working again. We have done all the routine lab solutions & have come up blank. The problem is our slides are showing little to no staining, where before there was a little differentiation & the abnormal cells stained fairly dark. If anyone has an image of a normal muscle & an abnormal muscle stained with this method it would be very helpful. Thanks for any help, Sharon Allen Neuropathology Lab HSC - Winnipeg, MB CA sallen@hsc.mb.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From SAllen <@t> exchange.hsc.mb.ca Thu Oct 30 15:09:40 2008 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Thu Oct 30 15:10:45 2008 Subject: [Histonet] Modified SDH Message-ID: Hi Histonet, I would like to hear from anyone who does the Modified SDH method. This is the method that uses Phenazine Methosulphate to suppress the normal mitochondria & stains only mitochondria with DNA mutations. We had been doing the test for about 5 years with good results, then we made up new solution & can't seem to get it working again. We have done all the routine lab solutions & have come up blank. The problem is our slides are showing little to no staining, where before there was a little differentiation & the abnormal cells stained fairly dark. If anyone has an image of a normal muscle & an abnormal muscle stained with this method it would be very helpful. Thanks for any help, Sharon Allen Neuropathology Lab HSC - Winnipeg, MB CA sallen@hsc.mb.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From dellav <@t> musc.edu Thu Oct 30 16:32:52 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Oct 30 16:33:00 2008 Subject: [Histonet] bone marrow biopsies In-Reply-To: <49098FD2.59AC.00AF.0@mercyhealth.com> References: <49098FD2.59AC.00AF.0@mercyhealth.com> Message-ID: You are being placed in a difficult position that will only translate into physician unhappiness, so even though they are in a hurry (and usually for valid clinical reasons) the fact is that compromises will affect something else. I'm assuming you are a one shift operation. My lab is 24 hours, which allows us sufficient time for fixation and decalcification while enabling slides to be out the next morning. While Dr. Richmond is correct that there are faster decalcifiers, I'm guessing they want immunohistochemistry in at least some cases and the mineral acids will destroy your hopes of good immuno staining. We use 10% formic acid and still have to decalcify for about six hours in order to have blocks that section well. If you had an evening shift I'd suggest shortening your processing time. Shortening fixation time is counter productive to good morphology and good IHC. Lastly, you don't indicate if your bone marrows arrive at all times of the day. If you are trying to complete fixation and decalcification on specimens arriving in the afternoon, all to be on the processor at the end of your shift, you end up in the position you find yourself currently, with blocks poorly decalcified that section poorly. The only way to make this work to everyone's satisfaction is to have a longer work day, if it is possible to stagger work shifts. This also presumes that you have multiple tissue processors and can place your bone marrows on a shorter cycle, perhaps along with other biopsies. Others here may be able to advise you regarding using microwave technology for fixation and decalcification which may ultimately save the day for you. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Thursday, October 30, 2008 11:44 AM To: histonet Subject: [Histonet] bone marrow biopsies We are currently using 10% formalin fixation on our bone marrow cores. We fix for 2 hours minimum prior to decal. We are using Immunocal from Decal Corp. for 2-4 hrs followed with processing overnight in VIP. Cores are still crunchy upon sectioning and we are doing surface decal for up to 30 min. Our paths want cores turned out within 24 hrs following procedure. Any suggestions? Thanks. Cindi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anhtx <@t> drreddys.com Fri Oct 31 01:49:57 2008 From: anhtx <@t> drreddys.com (anhtx@drreddys.com) Date: Fri Oct 31 01:42:17 2008 Subject: [Histonet] Microtome calibration Message-ID: Dear all Is anyone practising microtome calibration for thickness of sections cut? Our GLP auditors are frequently asking for it. They also suggest that automatic tissue processor time in each reagent should be calibrated. any comments Regards Dr Girish India Disclaimer This message contains legally privileged and/or confidential information. If you are not the intended recipient(s), or employee or agent responsible for delivery of this message to the intended recipient(s), you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this message in error, please immediately notify the sender and delete this e-mail message from your computer. WARNING: Computer viruses can be transmitted via email. The recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email. From SharonC <@t> celligent.net Fri Oct 31 04:52:46 2008 From: SharonC <@t> celligent.net (Sharon Campbell) Date: Fri Oct 31 04:52:55 2008 Subject: [Histonet] PIN-4 controls Message-ID: Happy Halloween! Does anyone know of a good PIN-4 control? We are currently using in-house tissue but are finding a lot of variance with the stainability. If anyone knows of a good source I would greatly appreciate it. Thanks in advance, Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 (704) 549-8444 x104 sharonc@celligent.net From rjbuesa <@t> yahoo.com Fri Oct 31 07:50:52 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 31 07:50:57 2008 Subject: [Histonet] Microtome calibration In-Reply-To: Message-ID: <634745.26049.qm@web65710.mail.ac4.yahoo.com> Dr. Girish: ? BOTH "requirements" are rubbish invented by bureaucrats with lots of time in their hands and trying to appear concerned and knowledgeable. ? Thickness is not necessary as long as the section is diagnostically useful or if some quantitative method as to the intensity is done in which case thickness = amount of matter, and would influence the outcome of the quantitative process. ? Timing the tissue processor is also totally irrelevant because it does not really matter a few minutes each way during a processing protocol. More important would be to keep a record of the fixation time that is the only step really critical in tissue processing. ? Ren? J. --- On Fri, 10/31/08, anhtx@drreddys.com wrote: From: anhtx@drreddys.com Subject: [Histonet] Microtome calibration To: histonet@lists.utsouthwestern.edu Date: Friday, October 31, 2008, 2:49 AM Dear all Is anyone practising microtome calibration for thickness of sections cut? Our GLP auditors are frequently asking for it. They also suggest that automatic tissue processor time in each reagent should be calibrated. any comments Regards Dr Girish India From godsgalnow <@t> aol.com Fri Oct 31 08:01:12 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Oct 31 08:01:47 2008 Subject: [Histonet] IHC equipment In-Reply-To: <939640.22785.qm@web36302.mail.mud.yahoo.com> References: <939640.22785.qm@web36302.mail.mud.yahoo.com> Message-ID: <8CB096FE25E43F6-128C-1FE4@webmail-dx07.sysops.aol.com> This is a matter of opinion.? DO you like a closed system or an open system?? My preference is an open system and I prefer Biocare.? As for the PIN-4 Cocktail, Biocare wins hands down there as well...as Dr Tacha works there. -----Original Message----- From: richard wells To: histonet Sent: Thu, 30 Oct 2008 2:17 pm Subject: [Histonet] IHC equipment I am helping to set up an in office lab for a group of urologists.? What is the best instrumentation for prostate tripple stain?? What are the best reagents? ? Test volume will be relatively low.? Perhaps as many as 5 additional immunostains will be added in the future.? Testing will be done by an experienced histotech with minimal exposure to immunoperoxidase techniques. ? Goals are to maximize simplicity, dependability and value. ? Thanks in advance for any and all advice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ktuttle <@t> umm.edu Fri Oct 31 08:29:43 2008 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Fri Oct 31 08:30:18 2008 Subject: [Histonet] pronase vs. proteinase-k Message-ID: <490AD006.90CE.001A.3@umm.edu> Are they the same ? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From kmerriam2003 <@t> yahoo.com Fri Oct 31 08:40:00 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Oct 31 08:40:07 2008 Subject: [Histonet] anti-mouse ferritin Message-ID: <321482.80739.qm@web50304.mail.re2.yahoo.com> Hi, ? Any recommedations for anti-mouse ferritin antibodies (looking to stain FFPE mouse tissue). ? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From nefff <@t> staff.uni-marburg.de Fri Oct 31 08:41:06 2008 From: nefff <@t> staff.uni-marburg.de (Dr. med. Frauke Neff) Date: Fri Oct 31 08:41:12 2008 Subject: [Histonet] Microtome calibration In-Reply-To: <634745.26049.qm@web65710.mail.ac4.yahoo.com> References: <634745.26049.qm@web65710.mail.ac4.yahoo.com> Message-ID: <1225460466.490b0af216448@webmail.med.uni-marburg.de> Dear Dr. Girish, I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if they are 1?m or 2.3?m thick. But I'm highly interested in how your GLP auditors want to calibrate the thickness of the section cuts. Are you supposed to measure the slides after cutting?! Before or after stretching in the water bath?! If they have a protocol how to do this I would be happy if they can share it with us. Frauke Quoting Rene J Buesa : > Dr. Girish: > > BOTH "requirements" are rubbish invented by bureaucrats with lots of time in > their hands and trying to appear concerned and knowledgeable. > > Thickness is not necessary as long as the section is diagnostically useful or > if some quantitative method as to the intensity is done in which case > thickness = amount of matter, and would influence the outcome of the > quantitative process. > > Timing the tissue processor is also totally irrelevant because it does not > really matter a few minutes each way during a processing protocol. More > important would be to keep a record of the fixation time that is the only > step really critical in tissue processing. > > Ren? J. > > --- On Fri, 10/31/08, anhtx@drreddys.com wrote: > > From: anhtx@drreddys.com > Subject: [Histonet] Microtome calibration > To: histonet@lists.utsouthwestern.edu > Date: Friday, October 31, 2008, 2:49 AM > > > > Dear all > > Is anyone practising microtome calibration for thickness of sections cut? > Our GLP auditors are frequently asking for it. > They also suggest that automatic tissue processor time in each reagent > should be calibrated. > > any comments > > Regards > Dr Girish > India > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From rjbuesa <@t> yahoo.com Fri Oct 31 09:07:39 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 31 09:07:42 2008 Subject: [Histonet] Microtome calibration In-Reply-To: <1225460466.490b0af216448@webmail.med.uni-marburg.de> Message-ID: <213222.54240.qm@web65702.mail.ac4.yahoo.com> Frauke: That calibration is ussually done by measuring the advance mechanism of the block holder and how many ?m the block moves towards the blade, but that is rubbish. Ren? J. --- On Fri, 10/31/08, Dr. med. Frauke Neff wrote: From: Dr. med. Frauke Neff Subject: Re: [Histonet] Microtome calibration To: rjbuesa@yahoo.com Cc: histonet@lists.utsouthwestern.edu, anhtx@drreddys.com Date: Friday, October 31, 2008, 9:41 AM Dear Dr. Girish, I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if they are 1?m or 2.3?m thick. But I'm highly interested in how your GLP auditors want to calibrate the thickness of the section cuts. Are you supposed to measure the slides after cutting?! Before or after stretching in the water bath?! If they have a protocol how to do this I would be happy if they can share it with us. Frauke Quoting Rene J Buesa : > Dr. Girish: > > BOTH "requirements" are rubbish invented by bureaucrats with lots of time in > their hands and trying to appear concerned and knowledgeable. > > Thickness is not necessary as long as the section is diagnostically useful or > if some quantitative method as to the intensity is done in which case > thickness = amount of matter, and would influence the outcome of the > quantitative process. > > Timing the tissue processor is also totally irrelevant because it does not > really matter a few minutes each way during a processing protocol. More > important would be to keep a record of the fixation time that is the only > step really critical in tissue processing. > > Ren? J. > > --- On Fri, 10/31/08, anhtx@drreddys.com wrote: > > From: anhtx@drreddys.com > Subject: [Histonet] Microtome calibration > To: histonet@lists.utsouthwestern.edu > Date: Friday, October 31, 2008, 2:49 AM > > > > Dear all > > Is anyone practising microtome calibration for thickness of sections cut? > Our GLP auditors are frequently asking for it. > They also suggest that automatic tissue processor time in each reagent > should be calibrated. > > any comments > > Regards > Dr Girish > India > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From pmarcum <@t> vet.upenn.edu Fri Oct 31 09:20:36 2008 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Fri Oct 31 09:20:44 2008 Subject: [Histonet] Microtome calibration In-Reply-To: <213222.54240.qm@web65702.mail.ac4.yahoo.com> References: <1225460466.490b0af216448@webmail.med.uni-marburg.de> <213222.54240.qm@web65702.mail.ac4.yahoo.com> Message-ID: <000801c93b63$d8072dc0$095a5b82@vet.upenn.edu> We have a GLP person who is not familiar with Histology at all. We have had to educate her about these issues and may other things. Calibration finally came down to having a PM on the units every year. After she understood all the things we do with a section during the cutting phase and pick up that would alter a true measurement. We also do MMA sections on the microtome and even those are next to impossible to measure due to some stretching of the section during pick up and drying. Oh Yes, the drying of the slides really put her in a tail spin as this was just not allowing the sections to stay the same as when they were picked up. Sometimes a long talk and demonstration is the only way to make the point that we are not standardized like clinical chemistry etc. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 31, 2008 10:08 AM To: Dr. med. Frauke Neff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome calibration Frauke: That calibration is ussually done by measuring the advance mechanism of the block holder and how many ?m the block moves towards the blade, but that is rubbish. Ren? J. --- On Fri, 10/31/08, Dr. med. Frauke Neff wrote: From: Dr. med. Frauke Neff Subject: Re: [Histonet] Microtome calibration To: rjbuesa@yahoo.com Cc: histonet@lists.utsouthwestern.edu, anhtx@drreddys.com Date: Friday, October 31, 2008, 9:41 AM Dear Dr. Girish, I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if they are 1?m or 2.3?m thick. But I'm highly interested in how your GLP auditors want to calibrate the thickness of the section cuts. Are you supposed to measure the slides after cutting?! Before or after stretching in the water bath?! If they have a protocol how to do this I would be happy if they can share it with us. Frauke Quoting Rene J Buesa : > Dr. Girish: > > BOTH "requirements" are rubbish invented by bureaucrats with lots of time in > their hands and trying to appear concerned and knowledgeable. > > Thickness is not necessary as long as the section is diagnostically useful or > if some quantitative method as to the intensity is done in which case > thickness = amount of matter, and would influence the outcome of the > quantitative process. > > Timing the tissue processor is also totally irrelevant because it does not > really matter a few minutes each way during a processing protocol. More > important would be to keep a record of the fixation time that is the only > step really critical in tissue processing. > > Ren? J. > > --- On Fri, 10/31/08, anhtx@drreddys.com wrote: > > From: anhtx@drreddys.com > Subject: [Histonet] Microtome calibration > To: histonet@lists.utsouthwestern.edu > Date: Friday, October 31, 2008, 2:49 AM > > > > Dear all > > Is anyone practising microtome calibration for thickness of sections cut? > Our GLP auditors are frequently asking for it. > They also suggest that automatic tissue processor time in each reagent > should be calibrated. > > any comments > > Regards > Dr Girish > India > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pattennj <@t> mail.nih.gov Fri Oct 31 10:01:50 2008 From: pattennj <@t> mail.nih.gov (Patten, Nicole (NIH/NIAAA) [F]) Date: Fri Oct 31 10:01:54 2008 Subject: [Histonet] Polyclonal Proteolipid Protein Ab? Message-ID: I have been (unsuccessfully) trying to find a polyclonal antibody against human proteolipid protein (stains myelin) that will work with immunofluorescence on FFPE sections. I have also tried some myelin basic protein with no luck. Has anyone found any in the past that have worked?? Thanks! Nicole J. Patten Post-Baccalaureate Fellow/IRTA NIAAA/National Institutes of Health From cheastys <@t> svm.vetmed.wisc.edu Fri Oct 31 10:51:52 2008 From: cheastys <@t> svm.vetmed.wisc.edu (Sandra Cheasty) Date: Fri Oct 31 10:52:19 2008 Subject: [Histonet] Formalin and Xylene Monitoring Badges Message-ID: Happy Halloween... I'm looking for a source of monitoring badges for formalin and xylene, both the 8 hour TWA and the STEL. Thank you, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680 From BoozerKA <@t> ah.org Fri Oct 31 11:21:49 2008 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Fri Oct 31 11:22:34 2008 Subject: [Histonet] Formalin and Xylene Monitoring Badges In-Reply-To: References: Message-ID: <490ACE2D.4AA8.00C0.0@ah.org> ASSAY TECHNOLOGY 1252 Quarry Lane Pleasanton, CA 94566 1-800-833-1258 >>> "Sandra Cheasty" 10/31/2008 08:51 >>> Happy Halloween... I'm looking for a source of monitoring badges for formalin and xylene, both the 8 hour TWA and the STEL. Thank you, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Oct 31 11:36:56 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Oct 31 11:35:41 2008 Subject: [Histonet] RE: Formalin and Xylene Monitoring Badges In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E3907B8F8687C@IBMB7Exchange.digestivespecialists.com> Try Mercedes Medical. http://www.mercedesmedical.com/ Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sandra Cheasty Sent: Friday, October 31, 2008 11:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin and Xylene Monitoring Badges Happy Halloween... I'm looking for a source of monitoring badges for formalin and xylene, both the 8 hour TWA and the STEL. Thank you, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwich <@t> 7thwavelabs.com Fri Oct 31 11:51:44 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Fri Oct 31 11:51:51 2008 Subject: [Histonet] Formalin and Xylene Monitoring Badges References: <490ACE2D.4AA8.00C0.0@ah.org> Message-ID: <62A8156F8071C8439080D626DF8C33A602E532@wave-mail.7thwave.local> Morphix Technologies www.morphtec.com 757-431-2260 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer Sent: Friday, October 31, 2008 11:22 AM To: histonet@lists.utsouthwestern.edu; Sandra Cheasty Subject: Re: [Histonet] Formalin and Xylene Monitoring Badges ASSAY TECHNOLOGY 1252 Quarry Lane Pleasanton, CA 94566 1-800-833-1258 >>> "Sandra Cheasty" 10/31/2008 08:51 >>> Happy Halloween... I'm looking for a source of monitoring badges for formalin and xylene, both the 8 hour TWA and the STEL. Thank you, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From jcline <@t> wchsys.org Fri Oct 31 12:02:23 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Oct 31 12:02:28 2008 Subject: [Histonet] Formalin and Xylene Monitoring Badges In-Reply-To: Message-ID: Sensors Safety Products Raleigh, N.C. 1-800-499-7232 Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sandra Cheasty Sent: Friday, October 31, 2008 11:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin and Xylene Monitoring Badges Happy Halloween... I'm looking for a source of monitoring badges for formalin and xylene, both the 8 hour TWA and the STEL. Thank you, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From tchistology <@t> earthlink.net Fri Oct 31 12:53:11 2008 From: tchistology <@t> earthlink.net (Randall Carpenter) Date: Fri Oct 31 12:53:16 2008 Subject: [Histonet] Re: Histonet Digest, Vol 59, Issue 40 Message-ID: <17333754.1225475591536.JavaMail.root@elwamui-milano.atl.sa.earthlink.net> Dr. Girish' I must respectfully disagree with those that consider calibrations of your microtome and stainer times "rubbish". I don't understand the reluctance of many of my histology colleagues to GLP compliance and to Quality Assurance. Firstly without QA there is no such thing as GLP compliance. I understand that sometimes QA people do not know much about histotechnique. At the same time they do know quite a bit about documentation and compliance. Most QA people are willing to listen, but again that should be a two way street. In the example of the microtome, Pamela's solution is perfect. If the person doing the PM uses calibrated and and traceable equipment and provides you with documentation, you're set. What you have shown QA and the FDA (when they show up) is that your microtome operates according to the manufacturer's specifications. That's it. We all know that the thickness of a section can vary (thick-thin), but a GLP based calibration only addresses the operating condition of your equipment. A "trained" histotechnician would be able to spot any problems in sectioning thickness. Yes? As far as stainer times are concerned, use a calibrated and traceable timer to check that the times match. Do it once, document it and you're done. Have QA check off on it and everybody is happy. I would suggest any lab that does a fair amount of GLP work do an IQ, OQ, PQ on their system. I know it can be a pain, but more and more CLIENTS are asking for it, not just QA. I understand that compliance can be more work. It is more work. What I don't understand is the reluctance of some to avoid making sure that their lab is in compliance with FDA or CAP. If your loved one had a biopsy come through a lab, how would you feel about someone using outdated reagents on that sample or using poorly maintained equipment? End communication. Randy Carpenter Twin Cities Histology > >Message: 18 >Date: Fri, 31 Oct 2008 10:20:36 -0400 >From: "Pamela Marcum" >Subject: RE: [Histonet] Microtome calibration >To: , "'Dr. med. Frauke Neff'" > >Cc: histonet@lists.utsouthwestern.edu >Message-ID: <000801c93b63$d8072dc0$095a5b82@vet.upenn.edu> >Content-Type: text/plain; charset="iso-8859-1" > >We have a GLP person who is not familiar with Histology at all. We have had >to educate her about these issues and may other things. Calibration finally >came down to having a PM on the units every year. After she understood all >the things we do with a section during the cutting phase and pick up that >would alter a true measurement. > >We also do MMA sections on the microtome and even those are next to >impossible to measure due to some stretching of the section during pick up >and drying. Oh Yes, the drying of the slides really put her in a tail spin >as this was just not allowing the sections to stay the same as when they >were picked up. > >Sometimes a long talk and demonstration is the only way to make the point >that we are not standardized like clinical chemistry etc. > >Pamela A Marcum >University of Pennsylvania >School of Veterinary Medicine >Comparative Orthopedic Laboratory (CORL) >382 W Street Rd >Kennett Square PA 19438 >610-925-6278 > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >Sent: Friday, October 31, 2008 10:08 AM >To: Dr. med. Frauke Neff >Cc: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Microtome calibration > >Frauke: >That calibration is ussually done by measuring the advance mechanism of the >block holder and how many ?m the block moves towards the blade, but that is >rubbish. >Ren? J. > >--- On Fri, 10/31/08, Dr. med. Frauke Neff >wrote: > >From: Dr. med. Frauke Neff >Subject: Re: [Histonet] Microtome calibration >To: rjbuesa@yahoo.com >Cc: histonet@lists.utsouthwestern.edu, anhtx@drreddys.com >Date: Friday, October 31, 2008, 9:41 AM > >Dear Dr. Girish, >I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if >they > are 1?m or 2.3?m thick. >But I'm highly interested in how your GLP auditors want to calibrate the >thickness of the section cuts. Are you supposed to measure the slides after >cutting?! Before or after stretching in the water bath?! If they have a >protocol how to do this I would be happy if they can share it with us. > >Frauke > > >Quoting Rene J Buesa : > >> Dr. Girish: >> >> BOTH "requirements" are rubbish invented by bureaucrats with >lots of time in >> their hands and trying to appear concerned and knowledgeable. >> >> Thickness is not necessary as long as the section is diagnostically useful >or >> if some quantitative method as to the intensity is done in which case >> thickness = amount of matter, and would influence the outcome of the >> quantitative process. >> >> Timing the tissue processor is also totally irrelevant because it does not >> really matter a few minutes each way during a processing protocol. More >> important would be to keep a record of the fixation time that is the only >> step really critical in tissue processing. >> >> Ren? J. >> >> --- On Fri, 10/31/08, anhtx@drreddys.com wrote: >> >> From: anhtx@drreddys.com >> Subject: [Histonet] Microtome calibration >> To: histonet@lists.utsouthwestern.edu >> Date: Friday, October 31, 2008, 2:49 AM >> >> >> >> Dear all >> >> Is anyone practising microtome calibration for thickness of sections cut? >> Our GLP auditors are frequently asking for it. >> They also suggest that automatic tissue processor time in each reagent >> should be calibrated. >> >> any comments >> >> Regards >> Dr Girish >> India >> >> >> >> From kenneth.a.troutman <@t> Vanderbilt.Edu Fri Oct 31 13:18:24 2008 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Fri Oct 31 13:18:29 2008 Subject: [Histonet] (no subject) Message-ID: <37DEF9AF72994947AF693956A59B9B660127FFF7@mailbe03.mc.vanderbilt.edu> Try the following decal from Surgipath. We used it with great success when I was in another lab. It is a 2 step decal procedure and it can significantly cut down on the total time. http://www.surgipath.com/region/us/product.aspx?p=77 Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Thursday, October 30, 2008 11:44 AM To: histonet Subject: [Histonet] bone marrow biopsies We are currently using 10% formalin fixation on our bone marrow cores. We fix for 2 hours minimum prior to decal. We are using Immunocal from Decal Corp. for 2-4 hrs followed with processing overnight in VIP. Cores are still crunchy upon sectioning and we are doing surface decal for up to 30 min. Our paths want cores turned out within 24 hrs following procedure. Any suggestions? Thanks. Cindi From kdboydhisto <@t> yahoo.com Fri Oct 31 14:08:08 2008 From: kdboydhisto <@t> yahoo.com (KELLY BOYD) Date: Fri Oct 31 14:08:14 2008 Subject: [Histonet] Re: Histonet Digest, Vol 59, Issue 40 In-Reply-To: <17333754.1225475591536.JavaMail.root@elwamui-milano.atl.sa.earthlink.net> Message-ID: <735996.36969.qm@web58608.mail.re3.yahoo.com> QA/QC?is a real pain at times, but it is priority in my lab.?I don't know any Histotech who likes all of the documentation!?I just recently had to have one of our five microtomes re-calibrated because it was producing slides that were staining much lighter than?from the other microtomes. (They are all set at?4 micrometers)?This particular microtome was also used for cutting all of our specials. It should be mandatory to have PMs (that include calibration) done on microtomes. How would an un-experienced tech know they were not cutting their specials at a certain thickness? I'll do all that extra "rubbish" anytime to make sure the patients are getting the best care they deserve. ? As far as fixation being the only?critical?part?in processing, That is "rubbish"!!!!!!! All steps are equally important. Maybe not to the exact minutes though. ? ? Happy Halloween to all!? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 Cell? (252)-943-9527 Fax? (252)-830-0032 ? ? --- On Fri, 10/31/08, Randall Carpenter wrote: From: Randall Carpenter Subject: [Histonet] Re: Histonet Digest, Vol 59, Issue 40 To: histonet@lists.utsouthwestern.edu Date: Friday, October 31, 2008, 1:53 PM Dr. Girish' I must respectfully disagree with those that consider calibrations of your microtome and stainer times "rubbish". I don't understand the reluctance of many of my histology colleagues to GLP compliance and to Quality Assurance. Firstly without QA there is no such thing as GLP compliance. I understand that sometimes QA people do not know much about histotechnique. At the same time they do know quite a bit about documentation and compliance. Most QA people are willing to listen, but again that should be a two way street. In the example of the microtome, Pamela's solution is perfect. If the person doing the PM uses calibrated and and traceable equipment and provides you with documentation, you're set. What you have shown QA and the FDA (when they show up) is that your microtome operates according to the manufacturer's specifications. That's it. We all know that the thickness of a section can vary (thick-thin), but a GLP based calibration only addresses the operating condition of your equipment. A "trained" histotechnician would be able to spot any problems in sectioning thickness. Yes? As far as stainer times are concerned, use a calibrated and traceable timer to check that the times match. Do it once, document it and you're done. Have QA check off on it and everybody is happy. I would suggest any lab that does a fair amount of GLP work do an IQ, OQ, PQ on their system. I know it can be a pain, but more and more CLIENTS are asking for it, not just QA. I understand that compliance can be more work. It is more work. What I don't understand is the reluctance of some to avoid making sure that their lab is in compliance with FDA or CAP. If your loved one had a biopsy come through a lab, how would you feel about someone using outdated reagents on that sample or using poorly maintained equipment? End communication. Randy Carpenter Twin Cities Histology > >Message: 18 >Date: Fri, 31 Oct 2008 10:20:36 -0400 >From: "Pamela Marcum" >Subject: RE: [Histonet] Microtome calibration >To: , "'Dr. med. Frauke Neff'" > >Cc: histonet@lists.utsouthwestern.edu >Message-ID: <000801c93b63$d8072dc0$095a5b82@vet.upenn.edu> >Content-Type: text/plain; charset="iso-8859-1" > >We have a GLP person who is not familiar with Histology at all. We have had >to educate her about these issues and may other things. Calibration finally >came down to having a PM on the units every year. After she understood all >the things we do with a section during the cutting phase and pick up that >would alter a true measurement. > >We also do MMA sections on the microtome and even those are next to >impossible to measure due to some stretching of the section during pick up >and drying. Oh Yes, the drying of the slides really put her in a tail spin >as this was just not allowing the sections to stay the same as when they >were picked up. > >Sometimes a long talk and demonstration is the only way to make the point >that we are not standardized like clinical chemistry etc. > >Pamela A Marcum >University of Pennsylvania >School of Veterinary Medicine >Comparative Orthopedic Laboratory (CORL) >382 W Street Rd >Kennett Square PA 19438 >610-925-6278 > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >Sent: Friday, October 31, 2008 10:08 AM >To: Dr. med. Frauke Neff >Cc: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Microtome calibration > >Frauke: >That calibration is ussually done by measuring the advance mechanism of the >block holder and how many ?m the block moves towards the blade, but that is >rubbish. >Ren? J. > >--- On Fri, 10/31/08, Dr. med. Frauke Neff >wrote: > >From: Dr. med. Frauke Neff >Subject: Re: [Histonet] Microtome calibration >To: rjbuesa@yahoo.com >Cc: histonet@lists.utsouthwestern.edu, anhtx@drreddys.com >Date: Friday, October 31, 2008, 9:41 AM > >Dear Dr. Girish, >I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if >they > are 1?m or 2.3?m thick. >But I'm highly interested in how your GLP auditors want to calibrate the >thickness of the section cuts. Are you supposed to measure the slides after >cutting?! Before or after stretching in the water bath?! If they have a >protocol how to do this I would be happy if they can share it with us. > >Frauke > > >Quoting Rene J Buesa : > >> Dr. Girish: >> >> BOTH "requirements" are rubbish invented by bureaucrats with >lots of time in >> their hands and trying to appear concerned and knowledgeable. >> >> Thickness is not necessary as long as the section is diagnostically useful >or >> if some quantitative method as to the intensity is done in which case >> thickness = amount of matter, and would influence the outcome of the >> quantitative process. >> >> Timing the tissue processor is also totally irrelevant because it does not >> really matter a few minutes each way during a processing protocol. More >> important would be to keep a record of the fixation time that is the only >> step really critical in tissue processing. >> >> Ren? J. >> >> --- On Fri, 10/31/08, anhtx@drreddys.com wrote: >> >> From: anhtx@drreddys.com >> Subject: [Histonet] Microtome calibration >> To: histonet@lists.utsouthwestern.edu >> Date: Friday, October 31, 2008, 2:49 AM >> >> >> >> Dear all >> >> Is anyone practising microtome calibration for thickness of sections cut? >> Our GLP auditors are frequently asking for it. >> They also suggest that automatic tissue processor time in each reagent >> should be calibrated. >> >> any comments >> >> Regards >> Dr Girish >> India >> >> >> >> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Oct 31 15:42:30 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Oct 31 15:42:34 2008 Subject: [Histonet] Formalin and Xylene Monitoring Badges In-Reply-To: Message-ID: <497295.9800.qm@web65703.mail.ac4.yahoo.com> Try a company named KEM Ren? J. --- On Fri, 10/31/08, Sandra Cheasty wrote: From: Sandra Cheasty Subject: [Histonet] Formalin and Xylene Monitoring Badges To: "histonet@lists.utsouthwestern.edu" Date: Friday, October 31, 2008, 11:51 AM Happy Halloween... I'm looking for a source of monitoring badges for formalin and xylene, both the 8 hour TWA and the STEL. Thank you, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Fri Oct 31 16:03:09 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri Oct 31 16:03:16 2008 Subject: [Histonet] mouse heart endothelium Message-ID: Is anyone routinely staining for mouse heart ECs in FFPE sections? If so, what markers and protocol are you using? Thanks, ANDREA -- From RSRICHMOND <@t> aol.com Fri Oct 31 20:17:31 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Oct 31 20:17:35 2008 Subject: [Histonet] Re: bone marrow biopsies Message-ID: Cindi Robinson replied to me offline, saying some of the things Vinnie della Speranza also said. I wasn't aware that hydrochloric acid decalcification (I assume that regular brand-name Decal is HCl) with prompt timing degraded IHC - something I guess that every lab has to determine for themselves. Is formic acid the decalcifier of choice for marrow cores? (One more reason to be using properly identified chemicals, and not secret proprietary mixtures, as John Kiernan has so often stressed on this list.) Repeating what I said before, I think that communication between histotechnologists and pathologists, and between pathologists and oncologists, is essential here. How often is overnight turnaround critical to patient care? Can the oncologists do biopsies earlier in the day when they need overnight turnaround? Actually, this isn't the worst communication problem I've seen among these groups of people. Even more serious is getting co-operation among all parties in getting marrow smears done right. In many services I've worked in, it's almost unheard of to get a marrow specimen with properly prepared and adequately stained smears. The procedure requires constant attention to detail, and frequent training of new workers by experienced technologists and - dare I say it? - pathologists. Bob Richmond Samurai Pathologist Knoxville TN