[Histonet] RE: Uneven H&E Staining

[Josie Britton]

Sounds to me that your having a de-paraffining problem.  Check your xylene or clearing agent, maybe there is water in them or they are not as clean as they should be.  Are you on a daily rotation/change of staining/de-paraffin reagents?  Maybe, your oven isn't hot enough to melt some paraffin off the slides before de-par!

 

Josie Britton HT  

 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Wednesday, November 19, 2008 9:48 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] RE: Uneven H&E Staining

 

Are the coplin jars all equally filled? If the wash-jars are partly filled,

the lower slides would be more differentiated than the upper.

Gudrun

 

-----Ursprüngliche Nachricht-----

Von: histonet-bounces <@t> lists.utsouthwestern.edu

[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Karla

Arrington

Gesendet: Mittwoch, 19. November 2008 02:34

An: histonet <@t> lists.utsouthwestern.edu

Betreff: [Histonet] RE: Uneven H&E Staining

 

Recently we have had an issue with uneven H&E staining.  Lately slides that

have 

three sections on one slide, mostly GI biopsies, and the last section

(bottom of 

slide) are paler than the upper two section.  All cut by the same person and

the 

stains were great.  What could be the cause of this?  Also with the quality

of 

formalin.  Can formalin be purchased as "bad" when it takes 6 days to fix

placenta 

or breasts?  What is the cause? 

 

Karla Arrington, HT(ASCP)

 

 

      

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