[Histonet] fluorescent labeling of starch

Bryan Llewellyn llewllew <@t> shaw.ca
Tue Nov 11 17:17:40 CST 2008


Try a PAS or a fluorescent PAS using Schiff's reagent made with acriflavine 
or acridine orange.

Bryan Llewellyn


----- Original Message ----- 
From: "Bill McManus" <bill.mcmanus <@t> usu.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, November 11, 2008 11:09 AM
Subject: [Histonet] fluorescent labeling of starch


> Hello All:
>
> I am new to this group, but my "sig. nif other" has been on the list  for 
> many years.  I am working with  student that is trying to stain  starches 
> ( rice, potato, etc.) for confocal.  Does anyone know of a  way to label 
> multiple starches, not just amylose but modified starches  such as amylose 
> pectin and other cross-linked starches with a single  dye?  If not, are 
> there other multiple dyes that would be worth a  try.  We are not budgeted 
> to produce antibodies at this time, maybe  later.
>
> Bill McManus
> Western Dairy Center
> Utah State University
> bill.mcmanus <@t> usu.edu
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