From anhtx <@t> drreddys.com Sat Nov 1 03:43:46 2008 From: anhtx <@t> drreddys.com (anhtx@drreddys.com) Date: Sat Nov 1 03:35:26 2008 Subject: [Histonet] Microtome calibration Message-ID: Dear Pamela/Rene/Frauke Thank you all for your comments and suggestions. As you are rightly saying , this is happening where an auditor is not aware of histological procedures.They just think of calibration/validation of any instrument which is part of GLP activities. We did ask them to tell us how to calibrate the microtome, They talk about block movement, measuring the block thickness with vernier caliperse (!??), one auditor even suggested to use fruits or rubber like material to measure the thickness of section cut. I think educating them is a better option than actually doing this useless exercise. We do have a quality check before the slides reach Pathologist for review. But these guys are not convinced. Thanks once again Regards Girish ----- Forwarded by ANHTX DR/DR/DRL/IN on 01/11/2008 02:00 PM ----- "Pamela Marcum" , "'Dr. med. > Frauke Neff'" Sent by: histonet-bo cc unces@lists histonet@lists.utsouthwestern.edu .utsouthwes Subject tern.edu RE: [Histonet] Microtome calibration 31/10/2008 07:50 PM We have a GLP person who is not familiar with Histology at all. We have had to educate her about these issues and may other things. Calibration finally came down to having a PM on the units every year. After she understood all the things we do with a section during the cutting phase and pick up that would alter a true measurement. We also do MMA sections on the microtome and even those are next to impossible to measure due to some stretching of the section during pick up and drying. Oh Yes, the drying of the slides really put her in a tail spin as this was just not allowing the sections to stay the same as when they were picked up. Sometimes a long talk and demonstration is the only way to make the point that we are not standardized like clinical chemistry etc. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, October 31, 2008 10:08 AM To: Dr. med. Frauke Neff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome calibration Frauke: That calibration is ussually done by measuring the advance mechanism of the block holder and how many ?m the block moves towards the blade, but that is rubbish. Ren? J. --- On Fri, 10/31/08, Dr. med. Frauke Neff wrote: From: Dr. med. Frauke Neff Subject: Re: [Histonet] Microtome calibration To: rjbuesa@yahoo.com Cc: histonet@lists.utsouthwestern.edu, anhtx@drreddys.com Date: Friday, October 31, 2008, 9:41 AM Dear Dr. Girish, I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if they are 1?m or 2.3?m thick. But I'm highly interested in how your GLP auditors want to calibrate the thickness of the section cuts. Are you supposed to measure the slides after cutting?! Before or after stretching in the water bath?! If they have a protocol how to do this I would be happy if they can share it with us. Frauke Quoting Rene J Buesa : > Dr. Girish: > > BOTH "requirements" are rubbish invented by bureaucrats with lots of time in > their hands and trying to appear concerned and knowledgeable. > > Thickness is not necessary as long as the section is diagnostically useful or > if some quantitative method as to the intensity is done in which case > thickness = amount of matter, and would influence the outcome of the > quantitative process. > > Timing the tissue processor is also totally irrelevant because it does not > really matter a few minutes each way during a processing protocol. More > important would be to keep a record of the fixation time that is the only > step really critical in tissue processing. > > Ren? J. > > --- On Fri, 10/31/08, anhtx@drreddys.com wrote: > > From: anhtx@drreddys.com > Subject: [Histonet] Microtome calibration > To: histonet@lists.utsouthwestern.edu > Date: Friday, October 31, 2008, 2:49 AM > > > > Dear all > > Is anyone practising microtome calibration for thickness of sections cut? > Our GLP auditors are frequently asking for it. > They also suggest that automatic tissue processor time in each reagent > should be calibrated. > > any comments > > Regards > Dr Girish > India > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer This message contains legally privileged and/or confidential information. 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From pruegg <@t> ihctech.net Sat Nov 1 12:18:28 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Nov 1 12:18:18 2008 Subject: [Histonet] (no subject) In-Reply-To: <37DEF9AF72994947AF693956A59B9B660127FFF7@mailbe03.mc.vanderbilt.edu> Message-ID: I speed up the fixation and decal time by using a platform shaker for both, it enhances the exchange, especially for decal, I would not skimp on fixation before decal, the fixation will protect the tissue from decal and processing, if you do not adequately fix, you can lose the proteins of interest during processing, and that is lose completely so that no retrieval process will work to bring them back. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troutman, Kenneth A Sent: Friday, October 31, 2008 12:18 PM To: Histonet Subject: [Histonet] (no subject) Try the following decal from Surgipath. We used it with great success when I was in another lab. It is a 2 step decal procedure and it can significantly cut down on the total time. http://www.surgipath.com/region/us/product.aspx?p=77 Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Thursday, October 30, 2008 11:44 AM To: histonet Subject: [Histonet] bone marrow biopsies We are currently using 10% formalin fixation on our bone marrow cores. We fix for 2 hours minimum prior to decal. We are using Immunocal from Decal Corp. for 2-4 hrs followed with processing overnight in VIP. Cores are still crunchy upon sectioning and we are doing surface decal for up to 30 min. Our paths want cores turned out within 24 hrs following procedure. Any suggestions? Thanks. Cindi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From raestask <@t> grics.net Sat Nov 1 13:33:00 2008 From: raestask <@t> grics.net (Rae Staskiewicz) Date: Sat Nov 1 13:33:16 2008 Subject: [Histonet] Fall Symposium Message-ID: <0B9D8A2C1ECA4FD2BB96C0BB442ECB1E@your4105e587b6> The Illinois Society for Histotechnologists is holding a one day seminar on November 8, 2008 at the Four Points by Sheraton in Fairview Heights, IL near St. Louis, MO. Get 2 workshops (6 CEUs) for only $35! Registration includes lunch. You can register on-line at www.ilhisto.org , or register on the day for only $10.00 additional. Workshops being offered are: Basic Dynamics of Fixation and Processing - Herbert Skip Brown Where Do I Find that Antibody and What Do I Do Once I have it? - Charlie Dorner From JMacDonald <@t> mtsac.edu Sun Nov 2 00:28:17 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun Nov 2 00:28:14 2008 Subject: [Histonet] California Society for Histotechnology annual symposium Message-ID: The California Society for Histotechnology is looking for speakers for the annual symposium. The dates are May 14-17, 2009 and the location is the Westin Hotel in Millbrae, CA (near the San Francisco airport) If you are interested please contact: Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From mikael.niku <@t> helsinki.fi Mon Nov 3 06:42:23 2008 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Mon Nov 3 06:42:35 2008 Subject: [Histonet] CD19 / CD20 cytoplasmic peptide abs? In-Reply-To: <0B9D8A2C1ECA4FD2BB96C0BB442ECB1E@your4105e587b6> References: <0B9D8A2C1ECA4FD2BB96C0BB442ECB1E@your4105e587b6> Message-ID: <011001c93db1$9ec16530$97a5d680@mmkem12636> Dear Histonetters, I'm looking for (peptide) antibodies against known cytoplasmic sequences of CD19 and/or CD20 (preferably murine or human antigen, but basically any mammalian species would do). In other words, I need to find mammal-crossreactive reagents for these targets. Any tips are greatly appreciated. With best regards, Mikael ----------------------------------------------------- Mikael Niku, PhD, university lecturer University of Helsinki, Division of Nutrition URL: mikael.nikunnakki.info - What do I think of western civilization? I think it would be a good idea! Gandhi ----------------------------------------------------- From ree3 <@t> leicester.ac.uk Mon Nov 3 08:08:46 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Nov 3 08:08:51 2008 Subject: [Histonet] double trouble Message-ID: <7722595275A4DD4FA225B92CDBF174A1744CF0B3F3@EXC-MBX3.cfs.le.ac.uk> OK chaps, which in your opinion is the best double labelling method when one is using primary antibodies raised in the same species, I am aware that Vector labs supply such a kit; many thanks. Cheers Richard Edwards University of Leicester U.K.... From contact <@t> excaliburpathology.com Mon Nov 3 08:23:08 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Nov 3 08:23:15 2008 Subject: [Histonet] double trouble Message-ID: <598577.66051.qm@web1102.biz.mail.sk1.yahoo.com> Incubate with one antibody and use DAB as the chromagen, then go back and incubate with the second antibody and use a colored chromagen such as AEC. Paula ----- Original Message ---- From: "Edwards, R.E.." To: histonet Sent: Monday, November 3, 2008 8:08:46 AM Subject: [Histonet] double trouble OK chaps, which in your opinion is the? best double labelling method when one? is? using primary antibodies raised in the? same? species, I am aware that Vector labs supply such a kit; many? thanks.. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Cheers ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Richard Edwards ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? University of Leicester ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? U.K.... ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Mon Nov 3 09:23:53 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Nov 3 09:24:19 2008 Subject: AW: [Histonet] double trouble In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1744CF0B3F3@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A1744CF0B3F3@EXC-MBX3.cfs.le.ac.uk> Message-ID: <5780821D94A5419A8FF01F0F6D5D09EE@dielangs.at> We do an automated doublestain, that works with Peroxidase-DAB and Alk. Phosphatase-Red. The critical point is, to perform a second HIER-step between the two detection-sequences. The fixed specimen don't suffer from the second heating, but the native antibodies are destroyed and are not detectable with the second-secondary-antibody. DAB sticks so well to the tissue, that it isn't washed away by this treatment. The nice think of this method is, that you don't have to think about species. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Edwards, R.E. Gesendet: Montag, 03. November 2008 15:09 An: histonet Betreff: [Histonet] double trouble OK chaps, which in your opinion is the best double labelling method when one is using primary antibodies raised in the same species, I am aware that Vector labs supply such a kit; many thanks. Cheers Richard Edwards University of Leicester U.K.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Mon Nov 3 09:49:26 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Nov 3 09:49:30 2008 Subject: [Histonet] double trouble References: <598577.66051.qm@web1102.biz.mail.sk1.yahoo.com> Message-ID: <322E670C37584817BCF1258690BC6A81@DHXTS541> Hopefully Chris van der Loos is looking in on this and will send the person who needs to do double IHC. He just published a very useful method in Journal of Histotechnology on this subject where the first antibody complex is removed, leaving behind chromogen after a retrieval method followed by the second antibody complex with a different chromogen. He may have a pdf of his publication available. Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Paula Pierce" To: "Edwards, R.E." ; "Histonet" Sent: Monday, November 03, 2008 7:23 AM Subject: Re: [Histonet] double trouble Incubate with one antibody and use DAB as the chromagen, then go back and incubate with the second antibody and use a colored chromagen such as AEC. Paula ----- Original Message ---- From: "Edwards, R.E.." To: histonet Sent: Monday, November 3, 2008 8:08:46 AM Subject: [Histonet] double trouble OK chaps, which in your opinion is the best double labelling method when one is using primary antibodies raised in the same species, I am aware that Vector labs supply such a kit; many thanks.. Cheers Richard Edwards University of Leicester U.K.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Mon Nov 3 09:53:47 2008 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Mon Nov 3 09:54:29 2008 Subject: [Histonet] double trouble References: <7722595275A4DD4FA225B92CDBF174A1744CF0B3F3@EXC-MBX3.cfs.le.ac.uk> Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB0028AE933@e2k3ms1.urmc-sh.rochester.edu> We have had much success with the Biocare line of double staining cocktails and ancillaries. Very easy to do Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Edwards, R.E. Sent: Mon 11/3/2008 9:08 AM To: histonet Subject: [Histonet] double trouble OK chaps, which in your opinion is the best double labelling method when one is using primary antibodies raised in the same species, I am aware that Vector labs supply such a kit; many thanks. Cheers Richard Edwards University of Leicester U.K.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstn192 <@t> yahoo.com Mon Nov 3 10:11:27 2008 From: jstn192 <@t> yahoo.com (Justin Thomas) Date: Mon Nov 3 10:11:31 2008 Subject: [Histonet] Presidential Voting Infomation Message-ID: <685769.76562.qm@web35702.mail.mud.yahoo.com> Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. ? The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. ? Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. ? PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE.? THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! From AGrobe2555 <@t> aol.com Mon Nov 3 10:15:14 2008 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Nov 3 10:15:21 2008 Subject: [Histonet] Complement activation and Thrombin generation Message-ID: Hello, I am looking for assays (either chromogenic or fluorescence based) for both complement activation and thrombin generation for a proposal. The ones suggested by the sponsor are not available in this country. Any suggestions/sources? Thanks, Albert **************Plan your next getaway with AOL Travel. Check out Today's Hot 5 Travel Deals! (http://pr.atwola.com/promoclk/100000075x1212416248x1200771803/aol?redir=http://travel.aol.com/discount-travel?ncid=emlcntustrav00000001) From ree3 <@t> leicester.ac.uk Mon Nov 3 10:19:39 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Nov 3 10:19:44 2008 Subject: [Histonet] Presidential Voting Infomation In-Reply-To: <685769.76562.qm@web35702.mail.mud.yahoo.com> References: <685769.76562.qm@web35702.mail.mud.yahoo.com> Message-ID: <7722595275A4DD4FA225B92CDBF174A1744CF0B3FD@EXC-MBX3.cfs.le.ac.uk> Thanks but no thanks.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Justin Thomas Sent: 03 November 2008 16:11 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. ? The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. ? Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. ? PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE.? THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Mon Nov 3 10:19:29 2008 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Mon Nov 3 10:19:47 2008 Subject: [Histonet] Presidential Voting Infomation In-Reply-To: <685769.76562.qm@web35702.mail.mud.yahoo.com> References: <685769.76562.qm@web35702.mail.mud.yahoo.com> Message-ID: I don't think the Histonet is appropriate for this type of Posting! Ruth Yaskovich N.I.H. -----Original Message----- From: Justin Thomas [mailto:jstn192@yahoo.com] Sent: Monday, November 03, 2008 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. ? The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. ? Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. ? PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE.? THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Mon Nov 3 10:20:16 2008 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Mon Nov 3 10:20:26 2008 Subject: [Histonet] Presidential Voting Infomation In-Reply-To: <685769.76562.qm@web35702.mail.mud.yahoo.com> References: <685769.76562.qm@web35702.mail.mud.yahoo.com> Message-ID: <000c01c93dd0$0ea11760$095a5b82@vet.upenn.edu> This totally inappropriate for HistoNet or any professional list server Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Justin Thomas Sent: Monday, November 03, 2008 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. ? The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. ? Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. ? PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE.? THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Mon Nov 3 10:21:45 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Mon Nov 3 10:22:15 2008 Subject: [Histonet] double trouble In-Reply-To: <598577.66051.qm@web1102.biz.mail.sk1.yahoo.com> References: <598577.66051.qm@web1102.biz.mail.sk1.yahoo.com> Message-ID: You cannot do what Paula recommends below without extra blocking steps, if the primaries are raised in the same species as the second secondary is raised. This is a rough outline of what I recommend: 1st primary antibody (X-anti-Y) 1st biotinylated secondary antibody (Fab anti-X) Streptavidin-HRP DAB+ H202 block (to block HRP) Avidin-biotin blocking kit (to block biotin from 1st secondary) Block with excess unlabeled Fab anti-X (to block any sites on 1st primary which might be bound by 2nd secondary) 2nd primary antibody (X anti-Z) 2nd biotinylated secondary antibody (Whole IgG anti-X - could also use Fab, it's just unnecessary/more expensive) Streptavidin-HRP Another HRP-based chromagen of your choosing (True Blue, Vector VIP, AEC+ etc) This can be made simpler by doing HRP and AP but I am not a fan of AP so I cannot personally recommend that. Good luck ... and the new protocol Gudrun mentioned that Chris published sounds very interesting! At 6:23 AM -0800 11/3/08, Paula Pierce wrote: >Incubate with one antibody and use DAB as the chromagen, then go >back and incubate with the second antibody and use a colored >chromagen such as AEC. Paula -- From Carol.Fields <@t> Northside.com Mon Nov 3 10:23:34 2008 From: Carol.Fields <@t> Northside.com (Carol Fields) Date: Mon Nov 3 10:24:07 2008 Subject: [Histonet] NOT APPROPRIATE Message-ID: <8CEB6DA1A3F35743800669D4CFE21F7D06DC2788@NSMXMS04.northside.local> This is totally not appropriate for the HistoNet. This fight would go on forever....... Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fields@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From Beatrice.Debrosse-Serra <@t> pfizer.com Mon Nov 3 10:24:49 2008 From: Beatrice.Debrosse-Serra <@t> pfizer.com (Debrosse-Serra, Beatrice) Date: Mon Nov 3 10:25:16 2008 Subject: [Histonet] Presidential Voting Infomation In-Reply-To: <685769.76562.qm@web35702.mail.mud.yahoo.com> Message-ID: <7B41B921086ADE4186377B8C33F702DE073631C4@lajamrexm01.amer.pfizer.com> This is very much uncalled for. This is not the place for political views. Keep them to yourself. Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Justin Thomas Sent: Monday, November 03, 2008 8:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. ? The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. ? Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. ? PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE.? THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Mon Nov 3 10:25:40 2008 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Nov 3 10:26:23 2008 Subject: [Histonet] Presidential Voting Infomation In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1744CF0B3FD@EXC-MBX3.cfs.le.ac.uk> References: <685769.76562.qm@web35702.mail.mud.yahoo.com>, <7722595275A4DD4FA225B92CDBF174A1744CF0B3FD@EXC-MBX3.cfs.le.ac.uk> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D2D14DB3A@LRGHEXVS1.practice.lrgh.org> And this deals with the sharing of Histology ideas How? Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. [ree3@leicester.ac.uk] Sent: Monday, November 03, 2008 11:19 AM To: 'jstn192@yahoo.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Presidential Voting Infomation Thanks but no thanks.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Justin Thomas Sent: 03 November 2008 16:11 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE. THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From SLB <@t> Stowers-Institute.org Mon Nov 3 10:28:49 2008 From: SLB <@t> Stowers-Institute.org (Beckham, Sharon) Date: Mon Nov 3 10:29:22 2008 Subject: [Histonet] Presidential Voting Infomation In-Reply-To: <685769.76562.qm@web35702.mail.mud.yahoo.com> Message-ID: Now, I think I've seen everything!! I can't believe you would use this venue for this trash. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Justin Thomas Sent: Monday, November 03, 2008 10:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE. THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Mon Nov 3 10:31:44 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Nov 3 10:31:50 2008 Subject: [Histonet] Presidential Voting Infomation In-Reply-To: <000c01c93dd0$0ea11760$095a5b82@vet.upenn.edu> References: <685769.76562.qm@web35702.mail.mud.yahoo.com> <000c01c93dd0$0ea11760$095a5b82@vet.upenn.edu> Message-ID: Gee, I really didn't know who I was going to vote for, being completely unaware of both candidates' platforms the day before I vote, but this random email to histonet totally swayed my decision! What a relief to know people like Justin care enough to tell me how to vote! Emily -- "You would know her for all the things she was...a woman who knew her way in and out of every new book without being singed, pinched, bumped or tickled by any line or chapter." John O'Hara, Appointment in Samarra From gvdobbin <@t> ihis.org Mon Nov 3 10:40:35 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Mon Nov 3 10:40:56 2008 Subject: [Histonet] Presidential Voting Infomation Message-ID: I don't remember seeing this persons name on the Histonet before. Could he be a Republican Party volunteer who joins various listservs for the sole purpose of garnering support for Mr. McCain?? It certainly would not surprise me. The fact that so many of us have read it already tells me he's accomplished his mission! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "And in the end it's not the years in your life that count. It's the life in your years." - Abraham Lincoln ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From jqb7 <@t> cdc.gov Mon Nov 3 10:43:33 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Nov 3 10:44:59 2008 Subject: [Histonet] Presidential Voting Infomation References: Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A73DF715@LTA3VS011.ees.hhs.gov> I received something similar but it was pro-Obama/anti-McCain on another listserv. I guess both parties are guilty. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Greg Dobbin Sent: Mon 11/3/2008 11:40 AM To: histonet@lists.utsouthwestern.edu; SLB@Stowers-Institute.org; jstn192@yahoo.com Subject: RE: [Histonet] Presidential Voting Infomation I don't remember seeing this persons name on the Histonet before. Could he be a Republican Party volunteer who joins various listservs for the sole purpose of garnering support for Mr. McCain?? It certainly would not surprise me. The fact that so many of us have read it already tells me he's accomplished his mission! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "And in the end it's not the years in your life that count. It's the life in your years." - Abraham Lincoln ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Mon Nov 3 10:56:30 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Nov 3 10:56:34 2008 Subject: [Histonet] Presidential Voting Infomation Message-ID: <520035.46395.qm@web1116.biz.mail.sk1.yahoo.com> I guess free speech is already dead. Go ask Joe the Plumber about his.. Justin, I suggest having chocolate chip cookies ready for when the KG.., oops, the FBI knocks on your door. ? ----- Original Message ---- From: Greg Dobbin To: histonet@lists.utsouthwestern.edu; SLB@Stowers-Institute.org; jstn192@yahoo.com Sent: Monday, November 3, 2008 10:40:35 AM Subject: RE: [Histonet] Presidential Voting Infomation I don't remember seeing this persons name on the Histonet before. Could he be a Republican Party volunteer who joins various listservs for the sole purpose of garnering support for Mr. McCain?? It certainly would not surprise me. The fact that so many of us have read it already tells me he's accomplished his mission! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE? ? C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "And in the end it's not the years in your life that count. It's the life in your years." - Abraham Lincoln ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Mon Nov 3 11:02:43 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Mon Nov 3 11:02:50 2008 Subject: [Histonet] Presidential Voting Infomation Message-ID: Honestly who cares. Let's just report to Listserv manager and move on. -- From gmartin <@t> marshallmedical.org Mon Nov 3 11:04:41 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Mon Nov 3 11:05:38 2008 Subject: [Histonet] Presidential Voting Information In-Reply-To: References: <685769.76562.qm@web35702.mail.mud.yahoo.com> Message-ID: <6ED9D4252F278841A0593D3D788AF24C03A5AAFD@mailsvr.MARSHMED.local> I absolutely agree ... THIS IS NO PLACE TO POST YOUR OPINON concerning politics !!!!!!!!!!!!!!!!!!!!!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) [E] Sent: Monday, November 03, 2008 8:19 AM To: jstn192@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Presidential Voting Infomation I don't think the Histonet is appropriate for this type of Posting! Ruth Yaskovich N.I.H. -----Original Message----- From: Justin Thomas [mailto:jstn192@yahoo.com] Sent: Monday, November 03, 2008 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. ? The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. ? Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. ? PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE.? THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Malcolm.McCallum <@t> tamut.edu Mon Nov 3 11:09:57 2008 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Mon Nov 3 11:11:28 2008 Subject: [Histonet] moderator!!! References: <685769.76562.qm@web35702.mail.mud.yahoo.com> <6ED9D4252F278841A0593D3D788AF24C03A5AAFD@mailsvr.MARSHMED.local> Message-ID: Can we cut the messages on how this message wasn't appropriate??? Or, at least send them to the original person? I have what a dozen messages exclaiming how inappropriate this message was!!! Frankly, the responses are more inconvenient than the original message! Malcolm L. McCallum Associate Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Fall Teaching Schedule & Office Hours: Ecology: M,W 1-2:40 pm Cell Biology: M 6-9:40 pm (don't ask!) Forensic Science: T,R 10-11:40am Office Hours: MW 12-1, 5-6, TR 11:40-12:30, "We live in a time when lemonade is made with artificial flavoring, and furnisher polish is made with fresh lemons." -Alfred E. Neuman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Martin, Gary Sent: Mon 11/3/2008 11:04 AM To: Yaskovich, Ruth A (NIH/NIDCR) [E]; jstn192@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Presidential Voting Information I absolutely agree ... THIS IS NO PLACE TO POST YOUR OPINON concerning politics !!!!!!!!!!!!!!!!!!!!!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) [E] Sent: Monday, November 03, 2008 8:19 AM To: jstn192@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Presidential Voting Infomation I don't think the Histonet is appropriate for this type of Posting! Ruth Yaskovich N.I.H. -----Original Message----- From: Justin Thomas [mailto:jstn192@yahoo.com] Sent: Monday, November 03, 2008 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. ? The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. ? Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. ? PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE.? THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Mon Nov 3 10:54:32 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Nov 3 11:14:27 2008 Subject: [Histonet] Presidential Voting Infomation In-Reply-To: <685769.76562.qm@web35702.mail.mud.yahoo.com> References: <685769.76562.qm@web35702.mail.mud.yahoo.com> Message-ID: <490F2CC8.6080404@umdnj.edu> Wow, where to start... lets see: 1) off-topic 3) factually incorrect 4) subjective and personally biased 5) abuse of ALL-CAPS 6) infuriatingly pandering This inappropriate to send out to such a list, John. Shame on you, you should know better. Justin Thomas wrote: > Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. > John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. > > The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. > > Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. > > > PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE. THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From NMP <@t> Stowers-Institute.org Mon Nov 3 11:19:18 2008 From: NMP <@t> Stowers-Institute.org (Marsh, Nannette) Date: Mon Nov 3 11:19:50 2008 Subject: [Histonet] Presidential Voting Infomation In-Reply-To: Message-ID: Emily, I love your response. There is no way this Justin person is a histotech because everyone knows how intelligent we are and we've all done our research and made the decision that is best for us. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Monday, November 03, 2008 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Presidential Voting Infomation Gee, I really didn't know who I was going to vote for, being completely unaware of both candidates' platforms the day before I vote, but this random email to histonet totally swayed my decision! What a relief to know people like Justin care enough to tell me how to vote! Emily -- "You would know her for all the things she was...a woman who knew her way in and out of every new book without being singed, pinched, bumped or tickled by any line or chapter." John O'Hara, Appointment in Samarra _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ruebenjcarter <@t> gmail.com Mon Nov 3 11:49:13 2008 From: ruebenjcarter <@t> gmail.com (R C) Date: Mon Nov 3 11:49:18 2008 Subject: [Histonet] ASCP HT maintenance fees Message-ID: <2a926e3f0811030949g8a5ac14q37bb30d3ac648957@mail.gmail.com> Can someone assist me in rationalize the annual cost of maintining HT certification (roughly $100 annually) and its benefit? Point accumulation is generally low for classes you must pay for, and those who obtained certification prior to 2004 are exempt. Should one not pay the annual fee, certification is dropped Is this correct?). In that case, can one advertise "HT" certification for future employment opportunities then, offer full explanation (and expired certification) during interview and that be sufficient? What I generally receive from ASCP is an annual bill and a random newsletter from time to time. Furthermore, when a bill isn't paid on time, the termingology in the subsequent bills become similar to that of a collection agency. Frankly, I find this mailing submission as well as state and national meetings more informative. Someone please clarify something I might be missing and any benefits of the "pay out." From DixonM <@t> vetmed.ufl.edu Mon Nov 3 11:56:28 2008 From: DixonM <@t> vetmed.ufl.edu (MaryAnn Dixon) Date: Mon Nov 3 11:56:34 2008 Subject: [Histonet] PGP 9.5 for intraepidermal nerve fibers Message-ID: Hi Netters, I'm stumped with a project and could really use your help. I have 3mm punches of skin that I need to stain with PGP 9.5 but I am specifically looking for the intraepidermal nerve fibers. The sections were given to me fixed in formalin and then I made cryo buttons out of them using OCT. I have found various articles that have cut sections ranging from 4 microns on up to 50 microns. I cut a slide to try at 20 microns but need help after this. I am using a polymer based detection system and the antibody is polyclonal from Dako. I know the antibody works well on paraffin embedded tissue but I'm lost with a starting point for these frozen 20um sections. Some journal articles also have the primary incubating overnight. Is this the only way I will be able to see the IENF's? If anyone out there is doing this kind of work can you please contact me. Thank you in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 From Jerry <@t> ralambusa.com Mon Nov 3 12:15:28 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Mon Nov 3 12:15:39 2008 Subject: [Histonet] Presidential Voting Infomation (Justin Thomas) Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B6010@server.ralambusa.com> This is just spam... Please delete this person. Thanks ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ From carrolpb <@t> umdnj.edu Mon Nov 3 10:57:53 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Nov 3 12:49:49 2008 Subject: [Histonet] Presidential Voting Infomation In-Reply-To: <685769.76562.qm@web35702.mail.mud.yahoo.com> References: <685769.76562.qm@web35702.mail.mud.yahoo.com> Message-ID: <490F2D91.802@umdnj.edu> curiously, the only other post on this list from this person was spam, hahahahaha! http://www.histosearch.com/histonet/Jun08A/RE.HistonetSUPPLYANDCHEMIA.html 0 for 2... nice! Justin Thomas wrote: > Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. > John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. > > The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. > > Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. > > > PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE. THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From ktuttle <@t> umm.edu Mon Nov 3 13:08:11 2008 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Mon Nov 3 13:09:02 2008 Subject: [Histonet] ASCP HT maintenance fees In-Reply-To: <2a926e3f0811030949g8a5ac14q37bb30d3ac648957@mail.gmail.com> References: <2a926e3f0811030949g8a5ac14q37bb30d3ac648957@mail.gmail.com> Message-ID: <490F05CA.90CE.001A.3@umm.edu> Really? I never pay to maintain HT certification. As far as I know theres a ASCP membership fee, but you dont have to be a member to be certified. Am I wrong here? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax >>> "R C" 11/3/2008 12:49 pm >>> Can someone assist me in rationalize the annual cost of maintining HT certification (roughly $100 annually) and its benefit? Point accumulation is generally low for classes you must pay for, and those who obtained certification prior to 2004 are exempt. Should one not pay the annual fee, certification is dropped Is this correct?). In that case, can one advertise "HT" certification for future employment opportunities then, offer full explanation (and expired certification) during interview and that be sufficient? What I generally receive from ASCP is an annual bill and a random newsletter from time to time. Furthermore, when a bill isn't paid on time, the termingology in the subsequent bills become similar to that of a collection agency. Frankly, I find this mailing submission as well as state and national meetings more informative. Someone please clarify something I might be missing and any benefits of the "pay out." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From DixonM <@t> vetmed.ufl.edu Mon Nov 3 13:41:51 2008 From: DixonM <@t> vetmed.ufl.edu (MaryAnn Dixon) Date: Mon Nov 3 13:41:57 2008 Subject: [Histonet] histology lab in New Dehli, India Message-ID: Hi Histonetters! I am looking for a good quality histopathological lab for animal tissues in New Dehli, India. Please contact me if any one knows of a reputable, high quality lab. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 From c.m.vanderloos <@t> amc.uva.nl Mon Nov 3 13:52:15 2008 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Mon Nov 3 13:52:39 2008 Subject: [Histonet] RE: double trouble Message-ID: <8be18ed727c02e0b.490f647f@amc.uva.nl> Hi Richard,As Gundrun lined out, a sequential method with a heating step in between to remove antibodies from the first staining sequence without affecting the DAB precipitate, is a very safe way to combine two antibodies from the same species. However, with this brown-red color combination one is unable to see sites of co-localization by mixed-color. Recently I published a method in JOH (September 2008) based on two alk phosp methods (Vector Blue and Dako's Liquid Permanent Red) that gives superior results in term of contrast between the basic colors and the purple mixed-color at sites of co-localization. The reaction product of Vector Blue as well as Liquid Permanent Red both survives the heat step without any changes. Cheers,Chris van der Loos, Amsterdam, Netherlands From Vickroy.Jim <@t> mhsil.com Mon Nov 3 13:57:50 2008 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Nov 3 13:58:36 2008 Subject: [Histonet] CELL CULTURE LINE ON SLIDES Message-ID: <24A4826E8EF0964D86BC5317306F58A52BA2A07DE2@mmc-mail.ad.mhsil.com> We were asked to do immunostains on slides where a particular cell line was growing. Can anyone tell me the best way to fix these slides before performing immunostains? We used to use acetone on cytospins but can't recall if there is a better way. thanks Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy.jim@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From marktarango <@t> gmail.com Mon Nov 3 14:02:04 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Nov 3 14:02:07 2008 Subject: [Histonet] NOT APPROPRIATE In-Reply-To: <8CEB6DA1A3F35743800669D4CFE21F7D06DC2788@NSMXMS04.northside.local> References: <8CEB6DA1A3F35743800669D4CFE21F7D06DC2788@NSMXMS04.northside.local> Message-ID: <5b6eb13e0811031202m22d5bb85i3071b948d9f98525@mail.gmail.com> Hopefully only until tomorrow. Perfect timing to bring it up is you ask me. Not much time left to debate it on the histonet. On 11/3/08, Carol Fields wrote: > > This is totally not appropriate for the HistoNet. This fight would go > on forever....... > > Carole Fields, HT (ASCP) > Histology Supervisor > Northside Hospital > Atlanta, GA 30342 > carol.fields@northside.com > > > > > > > > CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by > Northside Hospital. It may contain information that is confidential, > privileged, proprietary, or otherwise legally exempt from disclosure. If you > are not the intended recipient, you are hereby notified that you are not > authorized to read, print, retain, copy or disseminate this message, any > part of it, or any attachments. If you have received this message in error, > please delete this message and any attachments from your system without > reading the content and notify the sender immediately of the inadvertent > transmission. There is no intent on the part of the sender to waive any > privilege. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tpodawiltz <@t> lrgh.org Mon Nov 3 14:06:39 2008 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Nov 3 14:06:45 2008 Subject: [Histonet] ASCP HT maintenance fees In-Reply-To: <490F05CA.90CE.001A.3@umm.edu> References: <2a926e3f0811030949g8a5ac14q37bb30d3ac648957@mail.gmail.com>, <490F05CA.90CE.001A.3@umm.edu> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D2D14DB3D@LRGHEXVS1.practice.lrgh.org> If you were certified in 2004 or after you need to turn in 36 credit hours of continuing education in order to maintain your certification. You do not need to be a member of ASCP to be certified, however you do get some free CE hours with your membership. My certification was in 85, so yes, I am one of the old farts that is exempt. However, I have stayed current with my education. even in the years that I did not practice Histology. As a supervisor, I would not look at a resume that had an expired certification. Right or wrong I would assume that, the applicant did not take this field seriously enough by letting their certification lapse. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle [ktuttle@umm.edu] Sent: Monday, November 03, 2008 2:08 PM To: R C; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP HT maintenance fees Really? I never pay to maintain HT certification. As far as I know theres a ASCP membership fee, but you dont have to be a member to be certified. Am I wrong here? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax >>> "R C" 11/3/2008 12:49 pm >>> Can someone assist me in rationalize the annual cost of maintining HT certification (roughly $100 annually) and its benefit? Point accumulation is generally low for classes you must pay for, and those who obtained certification prior to 2004 are exempt. Should one not pay the annual fee, certification is dropped Is this correct?). In that case, can one advertise "HT" certification for future employment opportunities then, offer full explanation (and expired certification) during interview and that be sufficient? What I generally receive from ASCP is an annual bill and a random newsletter from time to time. Furthermore, when a bill isn't paid on time, the termingology in the subsequent bills become similar to that of a collection agency. Frankly, I find this mailing submission as well as state and national meetings more informative. Someone please clarify something I might be missing and any benefits of the "pay out." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From we3smitty <@t> yahoo.com Mon Nov 3 14:19:26 2008 From: we3smitty <@t> yahoo.com (angela smith) Date: Mon Nov 3 14:19:29 2008 Subject: [Histonet] Leica Bond Message-ID: <57511.64071.qm@web62001.mail.re1.yahoo.com> I have been evaluating the Leica Bond IHC stainer and their tech support Pauline Wong is absolutely wonderful. Will also be evaluating the Intellipath by Biocare in December. Any comments greatly appreciated! ? Thank you, Angela From juditw <@t> u.washington.edu Mon Nov 3 14:22:30 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Mon Nov 3 14:22:39 2008 Subject: [Histonet] Presidential Voting Infomation In-Reply-To: Message-ID: DO NOT POST POLITICAL OPINIONS ON HISTONET- THAT IS NOT WHAT IT IS FOR!!!! On Mon, 3 Nov 2008, Yaskovich, Ruth A (NIH/NIDCR) [E] wrote: > I don't think the Histonet is appropriate for this type of Posting! > Ruth Yaskovich > N.I.H. > > -----Original Message----- > From: Justin Thomas [mailto:jstn192@yahoo.com] > Sent: Monday, November 03, 2008 11:11 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Presidential Voting Infomation > > Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. > John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. > ? > The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. > ? > Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. > > ? > PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE.? THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From denise.woodward <@t> uconn.edu Mon Nov 3 15:02:40 2008 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Mon Nov 3 15:02:45 2008 Subject: [Histonet] EIER before HIER?? Message-ID: <40AC6D73C2B95C4CA21B26B7BF380C4002D0E48E@EXCHANGED.mgmt.ad.uconn.edu> Hi folks, I have a new antibody that seems to need both Protease digestion and Citrate buffer antigen retrieval. Does anyone have any experience with this double whammy approach and know if it works best to do EIER before HIER or HIER before EIER. Perhaps it doesn't matter which is first? Any comments?? Thanks, Denise Long Woodward University of Connecticut Dept. of Pathobiology and Veterinary Science From LSebree <@t> uwhealth.org Mon Nov 3 15:36:05 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Mon Nov 3 15:36:11 2008 Subject: [Histonet] EIER before HIER?? In-Reply-To: <40AC6D73C2B95C4CA21B26B7BF380C4002D0E48E@EXCHANGED.mgmt.ad.uconn.edu> Message-ID: I believe that when we've had to do this in the past, we've done EIER followed by HIER because that's the order it occurs on Ventana instruments. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Woodward, Denise Sent: Monday, November 03, 2008 3:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EIER before HIER?? Hi folks, I have a new antibody that seems to need both Protease digestion and Citrate buffer antigen retrieval. Does anyone have any experience with this double whammy approach and know if it works best to do EIER before HIER or HIER before EIER. Perhaps it doesn't matter which is first? Any comments?? Thanks, Denise Long Woodward University of Connecticut Dept. of Pathobiology and Veterinary Science _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Mon Nov 3 15:42:56 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon Nov 3 15:43:51 2008 Subject: [Histonet] Presidential Voting Infomation In-Reply-To: <685769.76562.qm@web35702.mail.mud.yahoo.com> References: <685769.76562.qm@web35702.mail.mud.yahoo.com> Message-ID: <2A582E8156B45F468A62D1F1D20AF083426864@EX-BE08.ohsu.edu> Justin, You have got to be kidding me...not a website to be pushing your political rhetoric. I got enough political paper to recycle in my mail at home, now I have to push the delete button. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Justin Thomas Sent: Monday, November 03, 2008 8:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. ? The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. ? Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. ? PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE.? THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Mon Nov 3 15:59:17 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Mon Nov 3 15:59:22 2008 Subject: [Histonet] ASCP HT maintenance fees In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638631D2D14DB3D@LRGHEXVS1.practice.lrgh.org> References: <2a926e3f0811030949g8a5ac14q37bb30d3ac648957@mail.gmail.com>, <490F05CA.90CE.001A.3@umm.edu> <38667E7FB77ECD4E91BFAEB8D98638631D2D14DB3D@LRGHEXVS1.practice.lrgh.org> Message-ID: I want to make you aware that JCAHO requires accredited facilities to undertake "primary source verification" for anyone claiming to have professional credentials. If I wanted to hire an applicant claiming to have histology certification it is my responsibility as manager to verify with ASCP that the individual does in fact have the certification. If you allowed your certification to lapse, it would become apparent when I contacted them about you and it would end any hope you had of obtaining a position with us. Only you can decide if the costs to maintain your certification are worth it but I thought you should be aware of the process that is followed to verify credentials. I agree with Tom that indicating that you allowed your certification to lapse would reflect negatively and likely sabotage hopes of employment, at least in some environments Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Monday, November 03, 2008 3:07 PM To: Kimberly Tuttle; R C; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP HT maintenance fees If you were certified in 2004 or after you need to turn in 36 credit hours of continuing education in order to maintain your certification. You do not need to be a member of ASCP to be certified, however you do get some free CE hours with your membership. My certification was in 85, so yes, I am one of the old farts that is exempt. However, I have stayed current with my education. even in the years that I did not practice Histology. As a supervisor, I would not look at a resume that had an expired certification. Right or wrong I would assume that, the applicant did not take this field seriously enough by letting their certification lapse. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle [ktuttle@umm.edu] Sent: Monday, November 03, 2008 2:08 PM To: R C; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP HT maintenance fees Really? I never pay to maintain HT certification. As far as I know theres a ASCP membership fee, but you dont have to be a member to be certified. Am I wrong here? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax >>> "R C" 11/3/2008 12:49 pm >>> Can someone assist me in rationalize the annual cost of maintining HT certification (roughly $100 annually) and its benefit? Point accumulation is generally low for classes you must pay for, and those who obtained certification prior to 2004 are exempt. Should one not pay the annual fee, certification is dropped Is this correct?). In that case, can one advertise "HT" certification for future employment opportunities then, offer full explanation (and expired certification) during interview and that be sufficient? What I generally receive from ASCP is an annual bill and a random newsletter from time to time. Furthermore, when a bill isn't paid on time, the termingology in the subsequent bills become similar to that of a collection agency. Frankly, I find this mailing submission as well as state and national meetings more informative. Someone please clarify something I might be missing and any benefits of the "pay out." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Mon Nov 3 16:06:03 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Nov 3 16:12:35 2008 Subject: [Histonet] Presidential Voting Infomation In-Reply-To: <3855F92002259948A66A8CA2D16E3A4F0B6010@server.ralambusa.com> Message-ID: >From down here in Australia, we hope it goes well. We are thinking of you. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Helisek Sent: Tuesday, 4 November 2008 5:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation (Justin Thomas) This is just spam... Please delete this person. Thanks ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From CIngles <@t> uwhealth.org Mon Nov 3 16:18:22 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Mon Nov 3 16:19:17 2008 Subject: [Histonet] Presidential Voting Infomation References: Message-ID: <08A0A863637F1349BBFD83A96B27A50A12019F@uwhis-xchng3.uwhis.hosp.wisc.edu> Thanks for the 'vote' of confidence. :) Claire >From down here in Australia, we hope it goes well. We are thinking of you. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From mabosso <@t> unipathllc.com Mon Nov 3 17:02:13 2008 From: mabosso <@t> unipathllc.com (Mary Abosso) Date: Mon Nov 3 17:02:52 2008 Subject: [Histonet] ASCP HT maintenance fees References: <2a926e3f0811030949g8a5ac14q37bb30d3ac648957@mail.gmail.com> <490F05CA.90CE.001A.3@umm.edu> Message-ID: <43A451981FF6634795BE83B1B5494D63136578@exchange.unipathllc.corp> Kim, That is my understanding as well. Mary Abosso ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kimberly Tuttle Sent: Mon 11/3/2008 12:08 PM To: R C; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP HT maintenance fees Really? I never pay to maintain HT certification. As far as I know theres a ASCP membership fee, but you dont have to be a member to be certified. Am I wrong here? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax >>> "R C" 11/3/2008 12:49 pm >>> Can someone assist me in rationalize the annual cost of maintining HT certification (roughly $100 annually) and its benefit? Point accumulation is generally low for classes you must pay for, and those who obtained certification prior to 2004 are exempt. Should one not pay the annual fee, certification is dropped Is this correct?). In that case, can one advertise "HT" certification for future employment opportunities then, offer full explanation (and expired certification) during interview and that be sufficient? What I generally receive from ASCP is an annual bill and a random newsletter from time to time. Furthermore, when a bill isn't paid on time, the termingology in the subsequent bills become similar to that of a collection agency. Frankly, I find this mailing submission as well as state and national meetings more informative. Someone please clarify something I might be missing and any benefits of the "pay out." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Mon Nov 3 17:04:19 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Nov 3 17:04:23 2008 Subject: [Histonet] CD19 / CD20 cytoplasmic peptide abs? References: <0B9D8A2C1ECA4FD2BB96C0BB442ECB1E@your4105e587b6> <011001c93db1$9ec16530$97a5d680@mmkem12636> Message-ID: Rabbit anti-CD20, B cell (Lab Vision/NeoMarkers; cat. #: RB-9013-P; Immunogen: synthetic peptide derived from C-terminus of human CD20 protein) This antibody has worked on every mammalian species on which I've tried it. Does not need ANY tissue pretreatment! Stains brilliantly. Jan Shivers IHC/Histo/EM Section Head Veterinary Diagnostic Laboratory University of Minnesota St. Paul, MN, USA ----- Original Message ----- From: "Mikael Niku" To: "'Histonet'" Sent: Monday, November 03, 2008 6:42 AM Subject: [Histonet] CD19 / CD20 cytoplasmic peptide abs? > Dear Histonetters, > > I'm looking for (peptide) antibodies against known cytoplasmic sequences > of > CD19 and/or CD20 (preferably murine or human antigen, but basically any > mammalian species would do). In other words, I need to find > mammal-crossreactive reagents for these targets. Any tips are greatly > appreciated. > > With best regards, > Mikael > > ----------------------------------------------------- > Mikael Niku, PhD, university lecturer > University of Helsinki, Division of Nutrition > URL: mikael.nikunnakki.info > > - What do I think of western civilization? > I think it would be a good idea! > Gandhi > ----------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ruebenjcarter <@t> gmail.com Mon Nov 3 17:28:43 2008 From: ruebenjcarter <@t> gmail.com (R C) Date: Mon Nov 3 17:28:49 2008 Subject: [Histonet] ASCP HT maintenance fees In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638631D2D14DB3D@LRGHEXVS1.practice.lrgh.org> References: <2a926e3f0811030949g8a5ac14q37bb30d3ac648957@mail.gmail.com> <490F05CA.90CE.001A.3@umm.edu> <38667E7FB77ECD4E91BFAEB8D98638631D2D14DB3D@LRGHEXVS1.practice.lrgh.org> Message-ID: <2a926e3f0811031528x60d57f15q9556368cc557f48b@mail.gmail.com> Thanks for the many replies however my objective hasn't been addressed. For the supervisors; My point is not to mislead and claim ASCP certification without having it. My question is to assist in rationalizing cost. Disregarding a credible applicant who doesn't see the financial investment towards ASCP shouldn't be interpreted as not being serious about a field. That ideology promotes "pay to play." It's a direct question towards ASCP membership feedback and prejudice (pre-2004 certification). As far as seriousness of the field, revert to my original objective of understanding what my dues fund, what do I as a tech directly receive in return, and why are older tech's exempt? The truth is many techs employed in clinical labs who do not have certification receive comparable salaries to those histotechs who study hard, pass the HT exam, and pay ASCP dues. Where is the justification in that? How does JACHO feel about that? I pose a credible question of fairness and return investment. Cash is king now and where I spend it is ever important. On Mon, Nov 3, 2008 at 12:06 PM, Podawiltz, Thomas wrote: > If you were certified in 2004 or after you need to turn in 36 credit hours > of continuing education in order to maintain your certification. You do not > need to be a member of ASCP to be certified, however you do get some free CE > hours with your membership. My certification was in 85, so yes, I am one of > the old farts that is exempt. However, I have stayed current with my > education. even in the years that I did not practice Histology. > > As a supervisor, I would not look at a resume that had an expired > certification. Right or wrong I would assume that, the applicant did not > take this field seriously enough by letting their certification lapse. > > Tom Podawiltz, HT (ASCP) > Histology Section Head/Laboratory Safety Officer > LRGHealthcare > 603-524-3211 ext: 3220 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle [ > ktuttle@umm.edu] > Sent: Monday, November 03, 2008 2:08 PM > To: R C; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] ASCP HT maintenance fees > > Really? I never pay to maintain HT certification. As far as I know theres a > ASCP membership fee, but you dont have to be a member to be certified. Am I > wrong here? > > Kimberly C. Tuttle HT (ASCP) > Pathology Biorepository and Research Core > University of Maryland > Room NBW58, UMMC > 22 S. Greene St > Baltimore, MD 21201 > (410) 328-5524 > (410) 328-5508 fax > > > >>> "R C" 11/3/2008 12:49 pm >>> > Can someone assist me in rationalize the annual cost of maintining HT > certification (roughly $100 annually) and its benefit? Point accumulation > is > generally low for classes you must pay for, and those who obtained > certification prior to 2004 are exempt. Should one not pay the annual fee, > certification is dropped Is this correct?). In that case, can one advertise > "HT" certification for future employment opportunities then, offer full > explanation (and expired certification) during interview and that be > sufficient? > > What I generally receive from ASCP is an annual bill and a random > newsletter > from time to time. Furthermore, when a bill isn't paid on time, > the termingology in the subsequent bills become similar to that of a > collection agency. Frankly, I find this mailing submission as well as state > and national meetings more informative. > > Someone please clarify something I might be missing and any benefits of the > "pay out." > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This e-mail and any accompanying attachments may be privileged, > confidential, contain protected health information about an identified > patient or be otherwise protected from disclosure. State and federal law > protect the confidentiality of this information. If the reader of this > message is not the intended recipient; you are prohibited from using, > disclosing, reproducing or distributing this information; you should > immediately notify the sender by telephone or e-mail and delete this e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential > information intended only for the use of the recipient(s) named above. If > you are not the intended recipient, you may not print,distribute, or copy > this message or any attachments. If you have received this communication in > error, please notify the sender by return e-mail and delete this message and > any attachments from your computer. Any views or opinions expressed are > solely those of the author and do not necessarily represent those of > LRGHealthcare. > > From Shirley.Chu <@t> moldev.com Mon Nov 3 19:09:48 2008 From: Shirley.Chu <@t> moldev.com (Chu, Shirley) Date: Mon Nov 3 19:11:19 2008 Subject: [Histonet] Advances in LCM and Microgenomics Research Seminar Message-ID: MDS Analytical Technologies will be hosting the following seminar at the Society for Neuroscience Meeting on Monday, November 12, 2008 Advances in LCM and Microgenomics Research Speakers include: * Charmaine Pietersen, Ph.D., McLean Hospital "Gene expression analysis in homogenous single cell populations in the superior temporal gyrus in postmortem brains from subjects with schizophrenia." * Jennifer A. Macdonald, Center for Vascular Biology and Department of Cell Biology, University of Connecticut Health Center "In situ analysis of blood-brain barrier gene expression using immuno-LCM coupled to qRT-PCR." For further information and to register for this seminar, please see the following website: http://www.moleculardevices.com/pages/lcm_at_neuroscience_11.2008.html Shirley Chu Application Scientist, Arcturus LCM Products Molecular Devices (now a part of MDS Analytical Technologies) 408-747-3765 | www.moleculardevices.com From djamesnz <@t> orcon.net.nz Mon Nov 3 19:18:12 2008 From: djamesnz <@t> orcon.net.nz (Darren James) Date: Mon Nov 3 19:18:40 2008 Subject: [Histonet] Presidential Voting Infomation In-Reply-To: Message-ID: ..and we here in NZ have our own government elections this weekend....fun times all over the world :-) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Tuesday, 4 November 2008 11:06 a.m. To: Jerry Helisek; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Presidential Voting Infomation >From down here in Australia, we hope it goes well. We are thinking of you. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Helisek Sent: Tuesday, 4 November 2008 5:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation (Justin Thomas) This is just spam... Please delete this person. Thanks ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Tue Nov 4 06:59:49 2008 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Tue Nov 4 06:59:58 2008 Subject: [Histonet] wavy mid myocardium Message-ID: Hi, I processed some mouse hearts that were in Z-Fix for 24 hrs. then put in 70% for anywhere from 24-48hrs. They were processed on a short run. The epicardium looks good but the mid myocardium is wavy. 15 min 80% 15 min 95 x 2 15 min 100% x 2 15 min Xylenes x2 10 min paraffin x 4 Any suggestions? Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) From HornHV <@t> archildrens.org Tue Nov 4 07:48:54 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Nov 4 07:48:59 2008 Subject: [Histonet] ASCP HT maintenance fees In-Reply-To: <43A451981FF6634795BE83B1B5494D63136578@exchange.unipathllc.corp> References: <2a926e3f0811030949g8a5ac14q37bb30d3ac648957@mail.gmail.com><490F05CA.90CE.001A.3@umm.edu> <43A451981FF6634795BE83B1B5494D63136578@exchange.unipathllc.corp> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82EC2@EMAIL.archildrens.org> If you were certified before 2004, it doesn't matter if you pay your dues or not. You are still certified. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Abosso Sent: Monday, November 03, 2008 5:02 PM To: Kimberly Tuttle; R C; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP HT maintenance fees Kim, That is my understanding as well. Mary Abosso ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kimberly Tuttle Sent: Mon 11/3/2008 12:08 PM To: R C; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP HT maintenance fees Really? I never pay to maintain HT certification. As far as I know theres a ASCP membership fee, but you dont have to be a member to be certified. Am I wrong here? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax >>> "R C" 11/3/2008 12:49 pm >>> Can someone assist me in rationalize the annual cost of maintining HT certification (roughly $100 annually) and its benefit? Point accumulation is generally low for classes you must pay for, and those who obtained certification prior to 2004 are exempt. Should one not pay the annual fee, certification is dropped Is this correct?). In that case, can one advertise "HT" certification for future employment opportunities then, offer full explanation (and expired certification) during interview and that be sufficient? What I generally receive from ASCP is an annual bill and a random newsletter from time to time. Furthermore, when a bill isn't paid on time, the termingology in the subsequent bills become similar to that of a collection agency. Frankly, I find this mailing submission as well as state and national meetings more informative. Someone please clarify something I might be missing and any benefits of the "pay out." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From sam.histology <@t> gmail.com Tue Nov 4 08:48:28 2008 From: sam.histology <@t> gmail.com (Sam Histology) Date: Tue Nov 4 08:48:35 2008 Subject: [Histonet] Be careful, be very careful when you Vote! Message-ID: Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann Dunham of Wichita, Kansas. (white atheist ). When Obama was two years old, his parents divorced and his father returned to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta with Obama when he was six years old. Within six months he had learned to speak the Indonesian language. Obama spent "two years in a Muslim school, then two more in a Catholic school" in Jakarta. Obama takes great care to conceal the fact that he is a Muslim while admitting that he was once a Muslim, mitigating that damning information by saying that, for two years, he also attended a Catholic school. Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, Sr. and Dunham divorced when Barack, Jr. was two. Obama's spinmeisters are now attempting to make it appear that Obama's introduction to Islam came from his father and that influence was temporary at best. In reality, the senior Obama returned to Kenya immediately following the divorce and never again had any direct influence over his son's education. Dunham married another Muslim, Lolo Soetoro who educated his stepson as a good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism is the radical teaching that created the Muslim terrorists who are now waging Jihad on the industrialized world. Since it is politically expedient to be a Christian when you are seeking political office in the United States, Obama joined the United Church of Christ to help purge any notion that he is still a Muslim. From sally.norton <@t> seattlechildrens.org Tue Nov 4 08:55:59 2008 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Tue Nov 4 08:56:07 2008 Subject: [Histonet] Be careful, be very careful when you Vote! In-Reply-To: References: Message-ID: <16E0693C7018C245959AC729FE66EDE52DBD09@s107.childrens.sea.kids> Think that the Histonetters site is the wrong place to be giving your political views. Sally Norton, Seattle, Wa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sam Histology Sent: Tuesday, November 04, 2008 6:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Be careful, be very careful when you Vote! Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann Dunham of Wichita, Kansas. (white atheist ). When Obama was two years old, his parents divorced and his father returned to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta with Obama when he was six years old. Within six months he had learned to speak the Indonesian language. Obama spent "two years in a Muslim school, then two more in a Catholic school" in Jakarta. Obama takes great care to conceal the fact that he is a Muslim while admitting that he was once a Muslim, mitigating that damning information by saying that, for two years, he also attended a Catholic school. Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, Sr. and Dunham divorced when Barack, Jr. was two. Obama's spinmeisters are now attempting to make it appear that Obama's introduction to Islam came from his father and that influence was temporary at best. In reality, the senior Obama returned to Kenya immediately following the divorce and never again had any direct influence over his son's education. Dunham married another Muslim, Lolo Soetoro who educated his stepson as a good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism is the radical teaching that created the Muslim terrorists who are now waging Jihad on the industrialized world. Since it is politically expedient to be a Christian when you are seeking political office in the United States, Obama joined the United Church of Christ to help purge any notion that he is still a Muslim. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From pruegg <@t> ihctech.net Tue Nov 4 08:56:03 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Nov 4 08:56:08 2008 Subject: [Histonet] ASCP HT maintenance fees In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82EC2@EMAIL.archildrens.org> Message-ID: Membership with ASCP is voluntary and is not connected to certification, once you are certified before 2004 you are certified for live. After 2004, ASCP now requires that you provide evidence of continuing education credits to maintain your certification each 5 years I believe, you will pay a fee to maintain but that is not the same thing as ASCP membership, that would be separate. I would encourage HT's to maintain a membership with ASCP for the other benefits our credentialing society offers other than certifying us. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, November 04, 2008 6:49 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP HT maintenance fees If you were certified before 2004, it doesn't matter if you pay your dues or not. You are still certified. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Abosso Sent: Monday, November 03, 2008 5:02 PM To: Kimberly Tuttle; R C; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP HT maintenance fees Kim, That is my understanding as well. Mary Abosso ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kimberly Tuttle Sent: Mon 11/3/2008 12:08 PM To: R C; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP HT maintenance fees Really? I never pay to maintain HT certification. As far as I know theres a ASCP membership fee, but you dont have to be a member to be certified. Am I wrong here? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax >>> "R C" 11/3/2008 12:49 pm >>> Can someone assist me in rationalize the annual cost of maintining HT certification (roughly $100 annually) and its benefit? Point accumulation is generally low for classes you must pay for, and those who obtained certification prior to 2004 are exempt. Should one not pay the annual fee, certification is dropped Is this correct?). In that case, can one advertise "HT" certification for future employment opportunities then, offer full explanation (and expired certification) during interview and that be sufficient? What I generally receive from ASCP is an annual bill and a random newsletter from time to time. Furthermore, when a bill isn't paid on time, the termingology in the subsequent bills become similar to that of a collection agency. Frankly, I find this mailing submission as well as state and national meetings more informative. Someone please clarify something I might be missing and any benefits of the "pay out." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Tue Nov 4 08:56:44 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Nov 4 08:56:59 2008 Subject: [Histonet] Be careful, be very careful when you Vote! In-Reply-To: References: Message-ID: <7722595275A4DD4FA225B92CDBF174A1744CF0B412@EXC-MBX3.cfs.le.ac.uk> Someone's getting pretty desperate!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sam Histology Sent: 04 November 2008 14:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Be careful, be very careful when you Vote! Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann Dunham of Wichita, Kansas. (white atheist ). When Obama was two years old, his parents divorced and his father returned to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta with Obama when he was six years old. Within six months he had learned to speak the Indonesian language. Obama spent "two years in a Muslim school, then two more in a Catholic school" in Jakarta. Obama takes great care to conceal the fact that he is a Muslim while admitting that he was once a Muslim, mitigating that damning information by saying that, for two years, he also attended a Catholic school. Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, Sr. and Dunham divorced when Barack, Jr. was two. Obama's spinmeisters are now attempting to make it appear that Obama's introduction to Islam came from his father and that influence was temporary at best. In reality, the senior Obama returned to Kenya immediately following the divorce and never again had any direct influence over his son's education. Dunham married another Muslim, Lolo Soetoro who educated his stepson as a good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism is the radical teaching that created the Muslim terrorists who are now waging Jihad on the industrialized world. Since it is politically expedient to be a Christian when you are seeking political office in the United States, Obama joined the United Church of Christ to help purge any notion that he is still a Muslim. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rfields <@t> gidocs.net Tue Nov 4 08:56:17 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Tue Nov 4 08:57:29 2008 Subject: [Histonet] ASCP HT maintenance fees Message-ID: <07732CE52EC3174AB891DE1C62DB4D8F43ECFD@GIEXCHANGE.gidocs.net> Direct quote from the ASCP website "ASCP membership is voluntary. Certification and maintenance of certification are not contingent upon membership in ASCP." The certification maintenance program cost is $50 every three years; and whatever your CMU's cost you. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R C Sent: Monday, November 03, 2008 5:29 PM To: Podawiltz, Thomas Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP HT maintenance fees Thanks for the many replies however my objective hasn't been addressed. For the supervisors; My point is not to mislead and claim ASCP certification without having it. My question is to assist in rationalizing cost. Disregarding a credible applicant who doesn't see the financial investment towards ASCP shouldn't be interpreted as not being serious about a field. That ideology promotes "pay to play." It's a direct question towards ASCP membership feedback and prejudice (pre-2004 certification). As far as seriousness of the field, revert to my original objective of understanding what my dues fund, what do I as a tech directly receive in return, and why are older tech's exempt? The truth is many techs employed in clinical labs who do not have certification receive comparable salaries to those histotechs who study hard, pass the HT exam, and pay ASCP dues. Where is the justification in that? How does JACHO feel about that? I pose a credible question of fairness and return investment. Cash is king now and where I spend it is ever important. On Mon, Nov 3, 2008 at 12:06 PM, Podawiltz, Thomas wrote: > If you were certified in 2004 or after you need to turn in 36 credit hours > of continuing education in order to maintain your certification. You do not > need to be a member of ASCP to be certified, however you do get some free CE > hours with your membership. My certification was in 85, so yes, I am one of > the old farts that is exempt. However, I have stayed current with my > education. even in the years that I did not practice Histology. > > As a supervisor, I would not look at a resume that had an expired > certification. Right or wrong I would assume that, the applicant did not > take this field seriously enough by letting their certification lapse. > > Tom Podawiltz, HT (ASCP) > Histology Section Head/Laboratory Safety Officer > LRGHealthcare > 603-524-3211 ext: 3220 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle [ > ktuttle@umm.edu] > Sent: Monday, November 03, 2008 2:08 PM > To: R C; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] ASCP HT maintenance fees > > Really? I never pay to maintain HT certification. As far as I know theres a > ASCP membership fee, but you dont have to be a member to be certified. Am I > wrong here? > > Kimberly C. Tuttle HT (ASCP) > Pathology Biorepository and Research Core > University of Maryland > Room NBW58, UMMC > 22 S. Greene St > Baltimore, MD 21201 > (410) 328-5524 > (410) 328-5508 fax > > > >>> "R C" 11/3/2008 12:49 pm >>> > Can someone assist me in rationalize the annual cost of maintining HT > certification (roughly $100 annually) and its benefit? Point accumulation > is > generally low for classes you must pay for, and those who obtained > certification prior to 2004 are exempt. Should one not pay the annual fee, > certification is dropped Is this correct?). In that case, can one advertise > "HT" certification for future employment opportunities then, offer full > explanation (and expired certification) during interview and that be > sufficient? > > What I generally receive from ASCP is an annual bill and a random > newsletter > from time to time. Furthermore, when a bill isn't paid on time, > the termingology in the subsequent bills become similar to that of a > collection agency. Frankly, I find this mailing submission as well as state > and national meetings more informative. > > Someone please clarify something I might be missing and any benefits of the > "pay out." > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This e-mail and any accompanying attachments may be privileged, > confidential, contain protected health information about an identified > patient or be otherwise protected from disclosure. State and federal law > protect the confidentiality of this information. If the reader of this > message is not the intended recipient; you are prohibited from using, > disclosing, reproducing or distributing this information; you should > immediately notify the sender by telephone or e-mail and delete this e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential > information intended only for the use of the recipient(s) named above. If > you are not the intended recipient, you may not print,distribute, or copy > this message or any attachments. If you have received this communication in > error, please notify the sender by return e-mail and delete this message and > any attachments from your computer. Any views or opinions expressed are > solely those of the author and do not necessarily represent those of > LRGHealthcare. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rfields <@t> gidocs.net Tue Nov 4 09:01:09 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Tue Nov 4 09:01:49 2008 Subject: [Histonet] Be careful, be very careful when you Vote! Message-ID: <07732CE52EC3174AB891DE1C62DB4D8F43ECFE@GIEXCHANGE.gidocs.net> Sam Histology; shame on you... seems you don't have a problem changing your name to get your political views onto histonet.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sam Histology Sent: Tuesday, November 04, 2008 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Be careful, be very careful when you Vote! Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann Dunham of Wichita, Kansas. (white atheist ). When Obama was two years old, his parents divorced and his father returned to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta with Obama when he was six years old. Within six months he had learned to speak the Indonesian language. Obama spent "two years in a Muslim school, then two more in a Catholic school" in Jakarta. Obama takes great care to conceal the fact that he is a Muslim while admitting that he was once a Muslim, mitigating that damning information by saying that, for two years, he also attended a Catholic school. Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, Sr. and Dunham divorced when Barack, Jr. was two. Obama's spinmeisters are now attempting to make it appear that Obama's introduction to Islam came from his father and that influence was temporary at best. In reality, the senior Obama returned to Kenya immediately following the divorce and never again had any direct influence over his son's education. Dunham married another Muslim, Lolo Soetoro who educated his stepson as a good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism is the radical teaching that created the Muslim terrorists who are now waging Jihad on the industrialized world. Since it is politically expedient to be a Christian when you are seeking political office in the United States, Obama joined the United Church of Christ to help purge any notion that he is still a Muslim. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Tue Nov 4 09:01:55 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Nov 4 09:02:00 2008 Subject: [Histonet] Be careful, be very careful when you Vote! In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1744CF0B412@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A1744CF0B412@EXC-MBX3.cfs.le.ac.uk> Message-ID: Wow, he's from the UK! He should know who to vote for! And his last name is Histology, so I instantly believe him. Emily -- "You would know her for all the things she was...a woman who knew her way in and out of every new book without being singed, pinched, bumped or tickled by any line or chapter." John O'Hara, Appointment in Samarra From slappycraw <@t> yahoo.com Tue Nov 4 09:03:18 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Tue Nov 4 09:03:25 2008 Subject: [Histonet] Be careful, be very careful when you Vote! Message-ID: <961941.27920.qm@web53601.mail.re2.yahoo.com> The politics of fear was last election so your about 4 years too late and full of whatever it is your trying to sell. ? Larry A. Woody Seattle, Wa. ________________________________ From: Sam Histology To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 4, 2008 6:48:28 AM Subject: [Histonet] Be careful, be very careful when you Vote! Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann Dunham of Wichita, Kansas. (white atheist ). When Obama was two years old, his parents divorced and his father returned to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta with Obama when he was six years old. Within six months he had learned to speak the Indonesian language. Obama spent "two years in a Muslim school, then two more in a Catholic school" in Jakarta. Obama takes great care to conceal the fact that he is a Muslim while admitting that he was once a Muslim, mitigating that damning information by saying that, for two years, he also attended a Catholic school. Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, Sr. and Dunham divorced when Barack, Jr. was two. Obama's spinmeisters are now attempting to make it appear that Obama's introduction to Islam came from his father and that influence was temporary at best. In reality, the senior Obama returned to Kenya immediately following the divorce and never again had any direct influence over his son's education. Dunham married another Muslim, Lolo Soetoro who educated his stepson as a good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism is the radical teaching that created the Muslim terrorists who are now waging Jihad on the industrialized world. Since it is politically expedient to be a Christian when you are seeking political office in the United States, Obama joined the United Church of Christ to help purge any notion that he is still a Muslim. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Tue Nov 4 09:06:20 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Nov 4 09:09:03 2008 Subject: [Histonet] Be careful, be very careful when you Vote! In-Reply-To: References: Message-ID: Sam Histology seems to be unfamiliar with Exodus 23:1. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sam Histology Sent: Tuesday, November 04, 2008 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Be careful, be very careful when you Vote! Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann Dunham of Wichita, Kansas. (white atheist ). When Obama was two years old, his parents divorced and his father returned to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta with Obama when he was six years old. Within six months he had learned to speak the Indonesian language. Obama spent "two years in a Muslim school, then two more in a Catholic school" in Jakarta. Obama takes great care to conceal the fact that he is a Muslim while admitting that he was once a Muslim, mitigating that damning information by saying that, for two years, he also attended a Catholic school. Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, Sr. and Dunham divorced when Barack, Jr. was two. Obama's spinmeisters are now attempting to make it appear that Obama's introduction to Islam came from his father and that influence was temporary at best. In reality, the senior Obama returned to Kenya immediately following the divorce and never again had any direct influence over his son's education. Dunham married another Muslim, Lolo Soetoro who educated his stepson as a good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism is the radical teaching that created the Muslim terrorists who are now waging Jihad on the industrialized world. Since it is politically expedient to be a Christian when you are seeking political office in the United States, Obama joined the United Church of Christ to help purge any notion that he is still a Muslim. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Tue Nov 4 09:12:59 2008 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue Nov 4 09:13:03 2008 Subject: [Histonet] ASCP HT maintenance fees In-Reply-To: <2a926e3f0811031528x60d57f15q9556368cc557f48b@mail.gmail.com> References: <2a926e3f0811030949g8a5ac14q37bb30d3ac648957@mail.gmail.com><490F05CA.90CE.001A.3@umm.edu><38667E7FB77ECD4E91BFAEB8D98638631D2D14DB3D@LRGHEXVS1.practice.lrgh.org> <2a926e3f0811031528x60d57f15q9556368cc557f48b@mail.gmail.com> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE607@EXCHANGEBE1.carle.com> Ruben, You do have valid questions and I guess the response depends on how you see the future. Histotechs are a hot commodity and some labs search for over a year to find one. In my experience, I have found certification in no way guarantees a competent technician but is a great, and necessary, starting point. I see the future as getting brighter and brighter for histotechs, both in pay and available schools. Many of these labs are starting to raise the bar on salary to attract the small supply of qualified techs. As pay goes up more schools will now find it profitable to supply the demand. Also as pay goes up you will see more and more employers require ASCP certification to validate their expense. I see us following the med tech profession and see certification as a future requirement both in state licensing and employer mandated. New York and Florida are good examples of what licensing does to the field. Maybe seeing someone that surrenders their certification as not serious about the field is a false assumption but I have to admit it would raise my eyebrows. I would scrutinize that applicant carefully and probably choose a certified applicant over them. The "pay to play" game is alive and well throughout medicine. I can't think of another profession in medicine that doesn't hit you with recurring licensing fees or continuing education requirements. I know it doesn't seem fair that we older techs came in before the maintenance part came along but ASCP had to start somewhere. Besides, we will all die off before you know it and then everyone will be paying the fee. Is it worth the money to maintain your certification? If you see the future as I do then it is essential if you want to stay in this field. If it lapses, how much would it cost both in money and effort to re-certify? The maintenance fee is a pittance compared to that. Charles Embrey, PA(ASCP), HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R C Sent: Monday, November 03, 2008 5:29 PM To: Podawiltz, Thomas Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP HT maintenance fees Thanks for the many replies however my objective hasn't been addressed. For the supervisors; My point is not to mislead and claim ASCP certification without having it. My question is to assist in rationalizing cost. Disregarding a credible applicant who doesn't see the financial investment towards ASCP shouldn't be interpreted as not being serious about a field. That ideology promotes "pay to play." It's a direct question towards ASCP membership feedback and prejudice (pre-2004 certification). As far as seriousness of the field, revert to my original objective of understanding what my dues fund, what do I as a tech directly receive in return, and why are older tech's exempt? The truth is many techs employed in clinical labs who do not have certification receive comparable salaries to those histotechs who study hard, pass the HT exam, and pay ASCP dues. Where is the justification in that? How does JACHO feel about that? I pose a credible question of fairness and return investment. Cash is king now and where I spend it is ever important. On Mon, Nov 3, 2008 at 12:06 PM, Podawiltz, Thomas wrote: > If you were certified in 2004 or after you need to turn in 36 credit hours > of continuing education in order to maintain your certification. You do not > need to be a member of ASCP to be certified, however you do get some free CE > hours with your membership. My certification was in 85, so yes, I am one of > the old farts that is exempt. However, I have stayed current with my > education. even in the years that I did not practice Histology. > > As a supervisor, I would not look at a resume that had an expired > certification. Right or wrong I would assume that, the applicant did not > take this field seriously enough by letting their certification lapse. > > Tom Podawiltz, HT (ASCP) > Histology Section Head/Laboratory Safety Officer > LRGHealthcare > 603-524-3211 ext: 3220 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle [ > ktuttle@umm.edu] > Sent: Monday, November 03, 2008 2:08 PM > To: R C; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] ASCP HT maintenance fees > > Really? I never pay to maintain HT certification. As far as I know theres a > ASCP membership fee, but you dont have to be a member to be certified. Am I > wrong here? > > Kimberly C. Tuttle HT (ASCP) > Pathology Biorepository and Research Core > University of Maryland > Room NBW58, UMMC > 22 S. Greene St > Baltimore, MD 21201 > (410) 328-5524 > (410) 328-5508 fax > > > >>> "R C" 11/3/2008 12:49 pm >>> > Can someone assist me in rationalize the annual cost of maintining HT > certification (roughly $100 annually) and its benefit? Point accumulation > is > generally low for classes you must pay for, and those who obtained > certification prior to 2004 are exempt. Should one not pay the annual fee, > certification is dropped Is this correct?). In that case, can one advertise > "HT" certification for future employment opportunities then, offer full > explanation (and expired certification) during interview and that be > sufficient? > > What I generally receive from ASCP is an annual bill and a random > newsletter > from time to time. Furthermore, when a bill isn't paid on time, > the termingology in the subsequent bills become similar to that of a > collection agency. Frankly, I find this mailing submission as well as state > and national meetings more informative. > > Someone please clarify something I might be missing and any benefits of the > "pay out." > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This e-mail and any accompanying attachments may be privileged, > confidential, contain protected health information about an identified > patient or be otherwise protected from disclosure. State and federal law > protect the confidentiality of this information. If the reader of this > message is not the intended recipient; you are prohibited from using, > disclosing, reproducing or distributing this information; you should > immediately notify the sender by telephone or e-mail and delete this e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential > information intended only for the use of the recipient(s) named above. If > you are not the intended recipient, you may not print,distribute, or copy > this message or any attachments. If you have received this communication in > error, please notify the sender by return e-mail and delete this message and > any attachments from your computer. Any views or opinions expressed are > solely those of the author and do not necessarily represent those of > LRGHealthcare. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Nov 4 08:58:43 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Nov 4 09:16:00 2008 Subject: [Histonet] Be careful, be very careful when you Vote! In-Reply-To: <16E0693C7018C245959AC729FE66EDE52DBD09@s107.childrens.sea.kids> References: <16E0693C7018C245959AC729FE66EDE52DBD09@s107.childrens.sea.kids> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C444@LTA3VS011.ees.hhs.gov> This is spam that has been sent to many listservs and is not from one of our fellow Histonetters. Please ignore. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norton, Sally Sent: Tuesday, November 04, 2008 9:56 AM To: Sam Histology; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Be careful, be very careful when you Vote! Think that the Histonetters site is the wrong place to be giving your political views. Sally Norton, Seattle, Wa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sam Histology Sent: Tuesday, November 04, 2008 6:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Be careful, be very careful when you Vote! Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann Dunham of Wichita, Kansas. (white atheist ). When Obama was two years old, his parents divorced and his father returned to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta with Obama when he was six years old. Within six months he had learned to speak the Indonesian language. Obama spent "two years in a Muslim school, then two more in a Catholic school" in Jakarta. Obama takes great care to conceal the fact that he is a Muslim while admitting that he was once a Muslim, mitigating that damning information by saying that, for two years, he also attended a Catholic school. Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, Sr. and Dunham divorced when Barack, Jr. was two. Obama's spinmeisters are now attempting to make it appear that Obama's introduction to Islam came from his father and that influence was temporary at best. In reality, the senior Obama returned to Kenya immediately following the divorce and never again had any direct influence over his son's education. Dunham married another Muslim, Lolo Soetoro who educated his stepson as a good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism is the radical teaching that created the Muslim terrorists who are now waging Jihad on the industrialized world. Since it is politically expedient to be a Christian when you are seeking political office in the United States, Obama joined the United Church of Christ to help purge any notion that he is still a Muslim. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Nov 4 09:23:02 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Nov 4 09:23:11 2008 Subject: [Histonet] ASCP HT maintenance fees In-Reply-To: <2a926e3f0811031528x60d57f15q9556368cc557f48b@mail.gmail.com> Message-ID: One of the benefits of ASCP membership is to support their efforts to fight for the medical technology profession in local and national legislation. With shortages, licensure, billing and all sorts of political issues that affect our lives we need someone to be working for us. CAP and CLIA does not do much for HT's since they still do not even require that a certified HT be doing the job. I get reports almost daily on what ASCP is doing for us and I support it by paying my membership dues. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R C Sent: Monday, November 03, 2008 4:29 PM To: Podawiltz, Thomas Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP HT maintenance fees Thanks for the many replies however my objective hasn't been addressed. For the supervisors; My point is not to mislead and claim ASCP certification without having it. My question is to assist in rationalizing cost. Disregarding a credible applicant who doesn't see the financial investment towards ASCP shouldn't be interpreted as not being serious about a field. That ideology promotes "pay to play." It's a direct question towards ASCP membership feedback and prejudice (pre-2004 certification). As far as seriousness of the field, revert to my original objective of understanding what my dues fund, what do I as a tech directly receive in return, and why are older tech's exempt? The truth is many techs employed in clinical labs who do not have certification receive comparable salaries to those histotechs who study hard, pass the HT exam, and pay ASCP dues. Where is the justification in that? How does JACHO feel about that? I pose a credible question of fairness and return investment. Cash is king now and where I spend it is ever important. On Mon, Nov 3, 2008 at 12:06 PM, Podawiltz, Thomas wrote: > If you were certified in 2004 or after you need to turn in 36 credit hours > of continuing education in order to maintain your certification. You do not > need to be a member of ASCP to be certified, however you do get some free CE > hours with your membership. My certification was in 85, so yes, I am one of > the old farts that is exempt. However, I have stayed current with my > education. even in the years that I did not practice Histology. > > As a supervisor, I would not look at a resume that had an expired > certification. Right or wrong I would assume that, the applicant did not > take this field seriously enough by letting their certification lapse. > > Tom Podawiltz, HT (ASCP) > Histology Section Head/Laboratory Safety Officer > LRGHealthcare > 603-524-3211 ext: 3220 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle [ > ktuttle@umm.edu] > Sent: Monday, November 03, 2008 2:08 PM > To: R C; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] ASCP HT maintenance fees > > Really? I never pay to maintain HT certification. As far as I know theres a > ASCP membership fee, but you dont have to be a member to be certified. Am I > wrong here? > > Kimberly C. Tuttle HT (ASCP) > Pathology Biorepository and Research Core > University of Maryland > Room NBW58, UMMC > 22 S. Greene St > Baltimore, MD 21201 > (410) 328-5524 > (410) 328-5508 fax > > > >>> "R C" 11/3/2008 12:49 pm >>> > Can someone assist me in rationalize the annual cost of maintining HT > certification (roughly $100 annually) and its benefit? Point accumulation > is > generally low for classes you must pay for, and those who obtained > certification prior to 2004 are exempt. Should one not pay the annual fee, > certification is dropped Is this correct?). In that case, can one advertise > "HT" certification for future employment opportunities then, offer full > explanation (and expired certification) during interview and that be > sufficient? > > What I generally receive from ASCP is an annual bill and a random > newsletter > from time to time. Furthermore, when a bill isn't paid on time, > the termingology in the subsequent bills become similar to that of a > collection agency. Frankly, I find this mailing submission as well as state > and national meetings more informative. > > Someone please clarify something I might be missing and any benefits of the > "pay out." > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This e-mail and any accompanying attachments may be privileged, > confidential, contain protected health information about an identified > patient or be otherwise protected from disclosure. State and federal law > protect the confidentiality of this information. If the reader of this > message is not the intended recipient; you are prohibited from using, > disclosing, reproducing or distributing this information; you should > immediately notify the sender by telephone or e-mail and delete this e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential > information intended only for the use of the recipient(s) named above. If > you are not the intended recipient, you may not print,distribute, or copy > this message or any attachments. If you have received this communication in > error, please notify the sender by return e-mail and delete this message and > any attachments from your computer. Any views or opinions expressed are > solely those of the author and do not necessarily represent those of > LRGHealthcare. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Tue Nov 4 09:29:10 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Nov 4 09:29:16 2008 Subject: [Histonet] Be careful, be very careful when you Vote! References: <7722595275A4DD4FA225B92CDBF174A1744CF0B412@EXC-MBX3.cfs.le.ac.uk> Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F57E@TRFT-EX01.xRothGen.nhs.uk> How do you know that Emily? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: 04 November 2008 15:02 To: Edwards, R.E.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Be careful, be very careful when you Vote! Wow, he's from the UK! He should know who to vote for! And his last name is Histology, so I instantly believe him. Emily -- "You would know her for all the things she was...a woman who knew her way in and out of every new book without being singed, pinched, bumped or tickled by any line or chapter." John O'Hara, Appointment in Samarra _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMP <@t> Stowers-Institute.org Tue Nov 4 10:05:26 2008 From: NMP <@t> Stowers-Institute.org (Marsh, Nannette) Date: Tue Nov 4 10:06:09 2008 Subject: [Histonet] Be careful, be very careful when you Vote! In-Reply-To: Message-ID: This is a question to someone in charge of the list server. Is there not a way to protect our server from this spam? I will of course just delete this, but I am curious. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Tuesday, November 04, 2008 9:06 AM To: 'Sam Histology' Cc: 'Histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Be careful, be very careful when you Vote! Sam Histology seems to be unfamiliar with Exodus 23:1. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sam Histology Sent: Tuesday, November 04, 2008 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Be careful, be very careful when you Vote! Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann Dunham of Wichita, Kansas. (white atheist ). When Obama was two years old, his parents divorced and his father returned to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta with Obama when he was six years old. Within six months he had learned to speak the Indonesian language. Obama spent "two years in a Muslim school, then two more in a Catholic school" in Jakarta. Obama takes great care to conceal the fact that he is a Muslim while admitting that he was once a Muslim, mitigating that damning information by saying that, for two years, he also attended a Catholic school. Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, Sr. and Dunham divorced when Barack, Jr. was two. Obama's spinmeisters are now attempting to make it appear that Obama's introduction to Islam came from his father and that influence was temporary at best. In reality, the senior Obama returned to Kenya immediately following the divorce and never again had any direct influence over his son's education. Dunham married another Muslim, Lolo Soetoro who educated his stepson as a good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism is the radical teaching that created the Muslim terrorists who are now waging Jihad on the industrialized world. Since it is politically expedient to be a Christian when you are seeking political office in the United States, Obama joined the United Church of Christ to help purge any notion that he is still a Muslim. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> georgetownhospitalsystem.org Tue Nov 4 10:09:33 2008 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Tue Nov 4 10:09:41 2008 Subject: [Histonet] Her2/Neu Valadation Message-ID: Dear Histonetters, How are you handling/answering the following question from the CAP checklist? Thanks in advance, Amy Georgetown Hospital System 843-527-7179 **NEW** 09/27/2007 ANP.22997 Phase I N/A YES NO If the laboratory assesses HER2 protein over-expression by immunohistochemistry (IHC) or HER2 gene amplification by fluorescence in situ hybridization (FISH), has the laboratory documented appropriate validation for the assay(s)? NOTE: Initial test validation must be performed on a minimum of 25 cases (recommended 25-100). Validation may be performed by comparing the results of testing with a validated alternative method (i.e., IHC vs. FISH) either in the same laboratory or another laboratory, or with the same validated method performed in another laboratory; validation testing must be done using the same set of cases in both labs. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From shive003 <@t> umn.edu Tue Nov 4 10:12:13 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Nov 4 10:12:18 2008 Subject: [Histonet] re: Biocare Decloaker HIER Message-ID: <394A9DE849AA4B6B9661DF4CF0C6274E@auxs.umn.edu> For those of you who use Biocare's Decloaker for HIER on regular cases (not prion protein retrieval), could you tell me how long you do HIER with these machines? I normally do my prion cases in a Decloaker for 20', with a 25' cooldown, but I suspect that regular tissues don't need that length of time at high pressure/temp. I have an extra Decloaker and am considering switching to this device for my normal case work HIER, if it's time efficient. Please reply privately. Thanks. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From akbitting <@t> geisinger.edu Tue Nov 4 10:14:44 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Nov 4 10:15:03 2008 Subject: [Histonet] Be careful, be very careful when you Vote! In-Reply-To: References: Message-ID: <49102EA4.2B7F.00C9.0@geisinger.edu> I'd say we all should be more worried about Revelations.... >>> "Smith, Allen" 11/4/2008 10:06 AM >>> Sam Histology seems to be unfamiliar with Exodus 23:1. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sam Histology Sent: Tuesday, November 04, 2008 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Be careful, be very careful when you Vote! Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann Dunham of Wichita, Kansas. (white atheist ). When Obama was two years old, his parents divorced and his father returned to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta with Obama when he was six years old. Within six months he had learned to speak the Indonesian language. Obama spent "two years in a Muslim school, then two more in a Catholic school" in Jakarta. Obama takes great care to conceal the fact that he is a Muslim while admitting that he was once a Muslim, mitigating that damning information by saying that, for two years, he also attended a Catholic school. Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, Sr. and Dunham divorced when Barack, Jr. was two. Obama's spinmeisters are now attempting to make it appear that Obama's introduction to Islam came from his father and that influence was temporary at best. In reality, the senior Obama returned to Kenya immediately following the divorce and never again had any direct influence over his son's education. Dunham married another Muslim, Lolo Soetoro who educated his stepson as a good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism is the radical teaching that created the Muslim terrorists who are now waging Jihad on the industrialized world. Since it is politically expedient to be a Christian when you are seeking political office in the United States, Obama joined the United Church of Christ to help purge any notion that he is still a Muslim. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From jqb7 <@t> cdc.gov Tue Nov 4 10:16:34 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Nov 4 10:16:46 2008 Subject: [Histonet] Be careful, be very careful when you Vote! In-Reply-To: <49102EA4.2B7F.00C9.0@geisinger.edu> References: <49102EA4.2B7F.00C9.0@geisinger.edu> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C44A@LTA3VS011.ees.hhs.gov> Good one! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Tuesday, November 04, 2008 11:15 AM To: 'Sam Histology'; Allen Smith Cc: 'Histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Be careful, be very careful when you Vote! I'd say we all should be more worried about Revelations.... >>> "Smith, Allen" 11/4/2008 10:06 AM >>> Sam Histology seems to be unfamiliar with Exodus 23:1. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sam Histology Sent: Tuesday, November 04, 2008 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Be careful, be very careful when you Vote! Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann Dunham of Wichita, Kansas. (white atheist ). When Obama was two years old, his parents divorced and his father returned to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta with Obama when he was six years old. Within six months he had learned to speak the Indonesian language. Obama spent "two years in a Muslim school, then two more in a Catholic school" in Jakarta. Obama takes great care to conceal the fact that he is a Muslim while admitting that he was once a Muslim, mitigating that damning information by saying that, for two years, he also attended a Catholic school. Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, Sr. and Dunham divorced when Barack, Jr. was two. Obama's spinmeisters are now attempting to make it appear that Obama's introduction to Islam came from his father and that influence was temporary at best. In reality, the senior Obama returned to Kenya immediately following the divorce and never again had any direct influence over his son's education. Dunham married another Muslim, Lolo Soetoro who educated his stepson as a good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism is the radical teaching that created the Muslim terrorists who are now waging Jihad on the industrialized world. Since it is politically expedient to be a Christian when you are seeking political office in the United States, Obama joined the United Church of Christ to help purge any notion that he is still a Muslim. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Tue Nov 4 10:24:32 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Nov 4 10:24:36 2008 Subject: [Histonet] re: Biocare Decloaker HIER In-Reply-To: <394A9DE849AA4B6B9661DF4CF0C6274E@auxs.umn.edu> Message-ID: I would say our "routine" HIER is 2 - 3 " in the decloaker after it gets up to temp. and then a cool down equivalent to however long it takes for the pressure to return to zero. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Tuesday, November 04, 2008 10:12 AM To: histonet Subject: [Histonet] re: Biocare Decloaker HIER For those of you who use Biocare's Decloaker for HIER on regular cases (not prion protein retrieval), could you tell me how long you do HIER with these machines? I normally do my prion cases in a Decloaker for 20', with a 25' cooldown, but I suspect that regular tissues don't need that length of time at high pressure/temp. I have an extra Decloaker and am considering switching to this device for my normal case work HIER, if it's time efficient. Please reply privately. Thanks. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmartin <@t> lrgh.org Tue Nov 4 10:26:49 2008 From: jmartin <@t> lrgh.org (Martin, Jessica) Date: Tue Nov 4 10:27:00 2008 Subject: [Histonet] RE: Histonet Digest, Vol 60, Issue 5 In-Reply-To: References: Message-ID: <53ADC5744236FC43B956FBCA32DEBBE3011EB5F7C2@LRGHEXVS2.practice.lrgh.org> Thanks for the many replies however my objective hasn't been addressed. For the supervisors; My point is not to mislead and claim ASCP certification without having it. My question is to assist in rationalizing cost. Disregarding a credible applicant who doesn't see the financial investment towards ASCP shouldn't be interpreted as not being serious about a field. That ideology promotes "pay to play." It's a direct question towards ASCP membership feedback and prejudice (pre-2004 certification). As far as seriousness of the field, revert to my original objective of understanding what my dues fund, what do I as a tech directly receive in return, and why are older tech's exempt? The truth is many techs employed in clinical labs who do not have certification receive comparable salaries to those histotechs who study hard, pass the HT exam, and pay ASCP dues. Where is the justification in that? How does JACHO feel about that? I pose a credible question of fairness and return investment. Cash is king now and where I spend it is ever important. I too have had a hard time trying to rationalize paying so much for dues. The annual fee that you are referring to is $89 or $94 depending on if you want to donate $5.00 to the ASCP Scholarship fund. I understand that this annual fee funds the ASCP magazine that is distributed a few times a year, provides funds for the ASCP advocacy group and to provide CE's for us. With your membership fee you get six "free" CE's per year. These CE's cost about $15 each. If you didn't get the membership these six CE's you would pay for out of your pocket anyway. This makes up for about $90 of your $94 fee. As far as having to pay a fee to stay certified, I think it is two separate things. The $94 annual fee is not connected to certification. This fee is your membership fee which is not required. There is a separate fee that you submit with your 36 CE's for the first three years that you are an ASCP member. If you have already been an ASCP member for three consecutive years then you submit the CMP voucher which waives the fee for certification. Another benefit of being an ASCP member is that you can get discounts on many workshops, education materials, and have your name added to the membership directory that is available to those who purchase it. Your name is out there for prospective employers to consider. Your point is well taken about fairness. It is advantageous to techs like us to maintain our certification, as many employers are not even considering candidates who are not certified. In the environment that I work in, we are required to maintain certification to stay employed. Eventually the "old farts" will be retiring and we will have assurance that our field has qualified histologists that have proven their value by participating in the CMP. As you know ASCP does not make it easy to get or stay certified these days. The fact that you are says a lot. I hope this helps. Jessica Martin HT, ASCP x-3231 jmartin@lrgh.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Tuesday, November 04, 2008 8:58 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 60, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Presidential Voting Infomation (Justin Thomas) (Jerry Helisek) 2. Re: Presidential Voting Infomation (Peter Carroll) 3. Re: ASCP HT maintenance fees (Kimberly Tuttle) 4. histology lab in New Dehli, India (MaryAnn Dixon) 5. RE: double trouble (C.M. van der Loos) 6. CELL CULTURE LINE ON SLIDES (Vickroy, Jim) 7. Re: NOT APPROPRIATE (Mark Tarango) 8. RE: ASCP HT maintenance fees (Podawiltz, Thomas) 9. Leica Bond (angela smith) 10. RE: Presidential Voting Infomation (Judith L. Williams) 11. EIER before HIER?? (Woodward, Denise) 12. RE: EIER before HIER?? (Sebree Linda A.) 13. RE: Presidential Voting Infomation (Robyn Vazquez) 14. RE: ASCP HT maintenance fees (Della Speranza, Vinnie) 15. RE: Presidential Voting Infomation (Tony Henwood) 16. RE: Presidential Voting Infomation (Ingles Claire) 17. RE: ASCP HT maintenance fees (Mary Abosso) 18. Re: CD19 / CD20 cytoplasmic peptide abs? (Jan Shivers) 19. Re: ASCP HT maintenance fees (R C) 20. Advances in LCM and Microgenomics Research Seminar (Chu, Shirley) 21. RE: Presidential Voting Infomation (Darren James) 22. wavy mid myocardium (Molinari, Betsy) 23. RE: ASCP HT maintenance fees (Horn, Hazel V) ---------------------------------------------------------------------- Message: 1 Date: Mon, 3 Nov 2008 13:15:28 -0500 From: "Jerry Helisek" Subject: [Histonet] Presidential Voting Infomation (Justin Thomas) To: Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B6010@server.ralambusa.com> Content-Type: text/plain; charset="us-ascii" This is just spam... Please delete this person. Thanks ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 2 Date: Mon, 03 Nov 2008 11:57:53 -0500 From: Peter Carroll Subject: Re: [Histonet] Presidential Voting Infomation To: histonet@lists.utsouthwestern.edu Message-ID: <490F2D91.802@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed curiously, the only other post on this list from this person was spam, hahahahaha! http://www.histosearch.com/histonet/Jun08A/RE.HistonetSUPPLYANDCHEMIA.html 0 for 2... nice! Justin Thomas wrote: > Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. > John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. > > The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. > > Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. > > > PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE. THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ Message: 3 Date: Mon, 03 Nov 2008 14:08:11 -0500 From: "Kimberly Tuttle" Subject: Re: [Histonet] ASCP HT maintenance fees To: "R C" , Message-ID: <490F05CA.90CE.001A.3@umm.edu> Content-Type: text/plain; charset=US-ASCII Really? I never pay to maintain HT certification. As far as I know theres a ASCP membership fee, but you dont have to be a member to be certified. Am I wrong here? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax >>> "R C" 11/3/2008 12:49 pm >>> Can someone assist me in rationalize the annual cost of maintining HT certification (roughly $100 annually) and its benefit? Point accumulation is generally low for classes you must pay for, and those who obtained certification prior to 2004 are exempt. Should one not pay the annual fee, certification is dropped Is this correct?). In that case, can one advertise "HT" certification for future employment opportunities then, offer full explanation (and expired certification) during interview and that be sufficient? What I generally receive from ASCP is an annual bill and a random newsletter from time to time. Furthermore, when a bill isn't paid on time, the termingology in the subsequent bills become similar to that of a collection agency. Frankly, I find this mailing submission as well as state and national meetings more informative. Someone please clarify something I might be missing and any benefits of the "pay out." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ------------------------------ Message: 4 Date: Mon, 3 Nov 2008 14:41:51 -0500 From: "MaryAnn Dixon" Subject: [Histonet] histology lab in New Dehli, India To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Histonetters! I am looking for a good quality histopathological lab for animal tissues in New Dehli, India. Please contact me if any one knows of a reputable, high quality lab. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 ------------------------------ Message: 5 Date: Mon, 03 Nov 2008 20:52:15 +0100 From: "C.M. van der Loos" Subject: [Histonet] RE: double trouble To: histonet@lists.utsouthwestern.edu Message-ID: <8be18ed727c02e0b.490f647f@amc.uva.nl> Content-Type: text/plain; charset=us-ascii Hi Richard,As Gundrun lined out, a sequential method with a heating step in between to remove antibodies from the first staining sequence without affecting the DAB precipitate, is a very safe way to combine two antibodies from the same species. However, with this brown-red color combination one is unable to see sites of co-localization by mixed-color. Recently I published a method in JOH (September 2008) based on two alk phosp methods (Vector Blue and Dako's Liquid Permanent Red) that gives superior results in term of contrast between the basic colors and the purple mixed-color at sites of co-localization. The reaction product of Vector Blue as well as Liquid Permanent Red both survives the heat step without any changes. Cheers,Chris van der Loos, Amsterdam, Netherlands ------------------------------ Message: 6 Date: Mon, 3 Nov 2008 13:57:50 -0600 From: "Vickroy, Jim" Subject: [Histonet] CELL CULTURE LINE ON SLIDES To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <24A4826E8EF0964D86BC5317306F58A52BA2A07DE2@mmc-mail.ad.mhsil.com> Content-Type: text/plain; charset="us-ascii" We were asked to do immunostains on slides where a particular cell line was growing. Can anyone tell me the best way to fix these slides before performing immunostains? We used to use acetone on cytospins but can't recall if there is a better way. thanks Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy.jim@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 7 Date: Mon, 3 Nov 2008 12:02:04 -0800 From: "Mark Tarango" Subject: Re: [Histonet] NOT APPROPRIATE To: "Carol Fields" Cc: histonet@lists.utsouthwestern.edu Message-ID: <5b6eb13e0811031202m22d5bb85i3071b948d9f98525@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hopefully only until tomorrow. Perfect timing to bring it up is you ask me. Not much time left to debate it on the histonet. On 11/3/08, Carol Fields wrote: > > This is totally not appropriate for the HistoNet. This fight would go > on forever....... > > Carole Fields, HT (ASCP) > Histology Supervisor > Northside Hospital > Atlanta, GA 30342 > carol.fields@northside.com > > > > > > > > CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by > Northside Hospital. It may contain information that is confidential, > privileged, proprietary, or otherwise legally exempt from disclosure. If you > are not the intended recipient, you are hereby notified that you are not > authorized to read, print, retain, copy or disseminate this message, any > part of it, or any attachments. If you have received this message in error, > please delete this message and any attachments from your system without > reading the content and notify the sender immediately of the inadvertent > transmission. There is no intent on the part of the sender to waive any > privilege. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Mon, 3 Nov 2008 15:06:39 -0500 From: "Podawiltz, Thomas" Subject: RE: [Histonet] ASCP HT maintenance fees To: Kimberly Tuttle , R C , "histonet@lists.utsouthwestern.edu" Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D2D14DB3D@LRGHEXVS1.practice.lrgh.org> Content-Type: text/plain; charset="us-ascii" If you were certified in 2004 or after you need to turn in 36 credit hours of continuing education in order to maintain your certification. You do not need to be a member of ASCP to be certified, however you do get some free CE hours with your membership. My certification was in 85, so yes, I am one of the old farts that is exempt. However, I have stayed current with my education. even in the years that I did not practice Histology. As a supervisor, I would not look at a resume that had an expired certification. Right or wrong I would assume that, the applicant did not take this field seriously enough by letting their certification lapse. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle [ktuttle@umm.edu] Sent: Monday, November 03, 2008 2:08 PM To: R C; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP HT maintenance fees Really? I never pay to maintain HT certification. As far as I know theres a ASCP membership fee, but you dont have to be a member to be certified. Am I wrong here? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax >>> "R C" 11/3/2008 12:49 pm >>> Can someone assist me in rationalize the annual cost of maintining HT certification (roughly $100 annually) and its benefit? Point accumulation is generally low for classes you must pay for, and those who obtained certification prior to 2004 are exempt. Should one not pay the annual fee, certification is dropped Is this correct?). In that case, can one advertise "HT" certification for future employment opportunities then, offer full explanation (and expired certification) during interview and that be sufficient? What I generally receive from ASCP is an annual bill and a random newsletter from time to time. Furthermore, when a bill isn't paid on time, the termingology in the subsequent bills become similar to that of a collection agency. Frankly, I find this mailing submission as well as state and national meetings more informative. Someone please clarify something I might be missing and any benefits of the "pay out." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ------------------------------ Message: 9 Date: Mon, 3 Nov 2008 12:19:26 -0800 (PST) From: angela smith Subject: [Histonet] Leica Bond To: histonet@lists.utsouthwestern.edu Message-ID: <57511.64071.qm@web62001.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I have been evaluating the Leica Bond IHC stainer and their tech support Pauline Wong is absolutely wonderful. Will also be evaluating the Intellipath by Biocare in December. Any comments greatly appreciated! ? Thank you, Angela ------------------------------ Message: 10 Date: Mon, 3 Nov 2008 12:22:30 -0800 (PST) From: "Judith L. Williams" Subject: RE: [Histonet] Presidential Voting Infomation To: "Yaskovich, Ruth A (NIH/NIDCR) [E]" Cc: histonet@lists.utsouthwestern.edu, jstn192@yahoo.com Message-ID: Content-Type: TEXT/PLAIN; charset=ISO-8859-1; format=flowed DO NOT POST POLITICAL OPINIONS ON HISTONET- THAT IS NOT WHAT IT IS FOR!!!! On Mon, 3 Nov 2008, Yaskovich, Ruth A (NIH/NIDCR) [E] wrote: > I don't think the Histonet is appropriate for this type of Posting! > Ruth Yaskovich > N.I.H. > > -----Original Message----- > From: Justin Thomas [mailto:jstn192@yahoo.com] > Sent: Monday, November 03, 2008 11:11 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Presidential Voting Infomation > > Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. > John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. > ? > The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. > ? > Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. > > ? > PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE.? THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 ------------------------------ Message: 11 Date: Mon, 3 Nov 2008 16:02:40 -0500 From: "Woodward, Denise" Subject: [Histonet] EIER before HIER?? To: Message-ID: <40AC6D73C2B95C4CA21B26B7BF380C4002D0E48E@EXCHANGED.mgmt.ad.uconn.edu> Content-Type: text/plain; charset="US-ASCII" Hi folks, I have a new antibody that seems to need both Protease digestion and Citrate buffer antigen retrieval. Does anyone have any experience with this double whammy approach and know if it works best to do EIER before HIER or HIER before EIER. Perhaps it doesn't matter which is first? Any comments?? Thanks, Denise Long Woodward University of Connecticut Dept. of Pathobiology and Veterinary Science ------------------------------ Message: 12 Date: Mon, 3 Nov 2008 15:36:05 -0600 From: "Sebree Linda A." Subject: RE: [Histonet] EIER before HIER?? To: "Woodward, Denise" , Message-ID: Content-Type: text/plain; charset="us-ascii" I believe that when we've had to do this in the past, we've done EIER followed by HIER because that's the order it occurs on Ventana instruments. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Woodward, Denise Sent: Monday, November 03, 2008 3:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EIER before HIER?? Hi folks, I have a new antibody that seems to need both Protease digestion and Citrate buffer antigen retrieval. Does anyone have any experience with this double whammy approach and know if it works best to do EIER before HIER or HIER before EIER. Perhaps it doesn't matter which is first? Any comments?? Thanks, Denise Long Woodward University of Connecticut Dept. of Pathobiology and Veterinary Science _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 3 Nov 2008 13:42:56 -0800 From: "Robyn Vazquez" Subject: RE: [Histonet] Presidential Voting Infomation To: jstn192@yahoo.com, histonet@lists.utsouthwestern.edu Message-ID: <2A582E8156B45F468A62D1F1D20AF083426864@EX-BE08.ohsu.edu> Content-Type: text/plain; charset=windows-1252 Justin, You have got to be kidding me...not a website to be pushing your political rhetoric. I got enough political paper to recycle in my mail at home, now I have to push the delete button. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Justin Thomas Sent: Monday, November 03, 2008 8:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. ? The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. ? Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. ? PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE.? THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 3 Nov 2008 16:59:17 -0500 From: "Della Speranza, Vinnie" Subject: RE: [Histonet] ASCP HT maintenance fees To: "'Podawiltz, Thomas'" , Kimberly Tuttle , R C , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I want to make you aware that JCAHO requires accredited facilities to undertake "primary source verification" for anyone claiming to have professional credentials. If I wanted to hire an applicant claiming to have histology certification it is my responsibility as manager to verify with ASCP that the individual does in fact have the certification. If you allowed your certification to lapse, it would become apparent when I contacted them about you and it would end any hope you had of obtaining a position with us. Only you can decide if the costs to maintain your certification are worth it but I thought you should be aware of the process that is followed to verify credentials. I agree with Tom that indicating that you allowed your certification to lapse would reflect negatively and likely sabotage hopes of employment, at least in some environments Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Monday, November 03, 2008 3:07 PM To: Kimberly Tuttle; R C; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP HT maintenance fees If you were certified in 2004 or after you need to turn in 36 credit hours of continuing education in order to maintain your certification. You do not need to be a member of ASCP to be certified, however you do get some free CE hours with your membership. My certification was in 85, so yes, I am one of the old farts that is exempt. However, I have stayed current with my education. even in the years that I did not practice Histology. As a supervisor, I would not look at a resume that had an expired certification. Right or wrong I would assume that, the applicant did not take this field seriously enough by letting their certification lapse. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle [ktuttle@umm.edu] Sent: Monday, November 03, 2008 2:08 PM To: R C; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP HT maintenance fees Really? I never pay to maintain HT certification. As far as I know theres a ASCP membership fee, but you dont have to be a member to be certified. Am I wrong here? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax >>> "R C" 11/3/2008 12:49 pm >>> Can someone assist me in rationalize the annual cost of maintining HT certification (roughly $100 annually) and its benefit? Point accumulation is generally low for classes you must pay for, and those who obtained certification prior to 2004 are exempt. Should one not pay the annual fee, certification is dropped Is this correct?). In that case, can one advertise "HT" certification for future employment opportunities then, offer full explanation (and expired certification) during interview and that be sufficient? What I generally receive from ASCP is an annual bill and a random newsletter from time to time. Furthermore, when a bill isn't paid on time, the termingology in the subsequent bills become similar to that of a collection agency. Frankly, I find this mailing submission as well as state and national meetings more informative. Someone please clarify something I might be missing and any benefits of the "pay out." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Tue, 4 Nov 2008 09:06:03 +1100 From: "Tony Henwood" Subject: RE: [Histonet] Presidential Voting Infomation To: "Jerry Helisek" , Message-ID: Content-Type: text/plain; charset="us-ascii" >From down here in Australia, we hope it goes well. We are thinking of you. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Helisek Sent: Tuesday, 4 November 2008 5:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation (Justin Thomas) This is just spam... Please delete this person. Thanks ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 16 Date: Mon, 3 Nov 2008 16:18:22 -0600 From: "Ingles Claire" Subject: RE: [Histonet] Presidential Voting Infomation To: Message-ID: <08A0A863637F1349BBFD83A96B27A50A12019F@uwhis-xchng3.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" Thanks for the 'vote' of confidence. :) Claire >From down here in Australia, we hope it goes well. We are thinking of you. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ------------------------------ Message: 17 Date: Mon, 3 Nov 2008 16:02:13 -0700 From: "Mary Abosso" Subject: RE: [Histonet] ASCP HT maintenance fees To: "Kimberly Tuttle" , "R C" , Message-ID: <43A451981FF6634795BE83B1B5494D63136578@exchange.unipathllc.corp> Content-Type: text/plain; charset="iso-8859-1" Kim, That is my understanding as well. Mary Abosso ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kimberly Tuttle Sent: Mon 11/3/2008 12:08 PM To: R C; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP HT maintenance fees Really? I never pay to maintain HT certification. As far as I know theres a ASCP membership fee, but you dont have to be a member to be certified. Am I wrong here? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax >>> "R C" 11/3/2008 12:49 pm >>> Can someone assist me in rationalize the annual cost of maintining HT certification (roughly $100 annually) and its benefit? Point accumulation is generally low for classes you must pay for, and those who obtained certification prior to 2004 are exempt. Should one not pay the annual fee, certification is dropped Is this correct?). In that case, can one advertise "HT" certification for future employment opportunities then, offer full explanation (and expired certification) during interview and that be sufficient? What I generally receive from ASCP is an annual bill and a random newsletter from time to time. Furthermore, when a bill isn't paid on time, the termingology in the subsequent bills become similar to that of a collection agency. Frankly, I find this mailing submission as well as state and national meetings more informative. Someone please clarify something I might be missing and any benefits of the "pay out." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Mon, 3 Nov 2008 17:04:19 -0600 From: "Jan Shivers" Subject: Re: [Histonet] CD19 / CD20 cytoplasmic peptide abs? To: "Mikael Niku" Cc: histonet Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Rabbit anti-CD20, B cell (Lab Vision/NeoMarkers; cat. #: RB-9013-P; Immunogen: synthetic peptide derived from C-terminus of human CD20 protein) This antibody has worked on every mammalian species on which I've tried it. Does not need ANY tissue pretreatment! Stains brilliantly. Jan Shivers IHC/Histo/EM Section Head Veterinary Diagnostic Laboratory University of Minnesota St. Paul, MN, USA ----- Original Message ----- From: "Mikael Niku" To: "'Histonet'" Sent: Monday, November 03, 2008 6:42 AM Subject: [Histonet] CD19 / CD20 cytoplasmic peptide abs? > Dear Histonetters, > > I'm looking for (peptide) antibodies against known cytoplasmic sequences > of > CD19 and/or CD20 (preferably murine or human antigen, but basically any > mammalian species would do). In other words, I need to find > mammal-crossreactive reagents for these targets. Any tips are greatly > appreciated. > > With best regards, > Mikael > > ----------------------------------------------------- > Mikael Niku, PhD, university lecturer > University of Helsinki, Division of Nutrition > URL: mikael.nikunnakki.info > > - What do I think of western civilization? > I think it would be a good idea! > Gandhi > ----------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 19 Date: Mon, 3 Nov 2008 15:28:43 -0800 From: "R C" Subject: Re: [Histonet] ASCP HT maintenance fees To: "Podawiltz, Thomas" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <2a926e3f0811031528x60d57f15q9556368cc557f48b@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Thanks for the many replies however my objective hasn't been addressed. For the supervisors; My point is not to mislead and claim ASCP certification without having it. My question is to assist in rationalizing cost. Disregarding a credible applicant who doesn't see the financial investment towards ASCP shouldn't be interpreted as not being serious about a field. That ideology promotes "pay to play." It's a direct question towards ASCP membership feedback and prejudice (pre-2004 certification). As far as seriousness of the field, revert to my original objective of understanding what my dues fund, what do I as a tech directly receive in return, and why are older tech's exempt? The truth is many techs employed in clinical labs who do not have certification receive comparable salaries to those histotechs who study hard, pass the HT exam, and pay ASCP dues. Where is the justification in that? How does JACHO feel about that? I pose a credible question of fairness and return investment. Cash is king now and where I spend it is ever important. On Mon, Nov 3, 2008 at 12:06 PM, Podawiltz, Thomas wrote: > If you were certified in 2004 or after you need to turn in 36 credit hours > of continuing education in order to maintain your certification. You do not > need to be a member of ASCP to be certified, however you do get some free CE > hours with your membership. My certification was in 85, so yes, I am one of > the old farts that is exempt. However, I have stayed current with my > education. even in the years that I did not practice Histology. > > As a supervisor, I would not look at a resume that had an expired > certification. Right or wrong I would assume that, the applicant did not > take this field seriously enough by letting their certification lapse. > > Tom Podawiltz, HT (ASCP) > Histology Section Head/Laboratory Safety Officer > LRGHealthcare > 603-524-3211 ext: 3220 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle [ > ktuttle@umm.edu] > Sent: Monday, November 03, 2008 2:08 PM > To: R C; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] ASCP HT maintenance fees > > Really? I never pay to maintain HT certification. As far as I know theres a > ASCP membership fee, but you dont have to be a member to be certified. Am I > wrong here? > > Kimberly C. Tuttle HT (ASCP) > Pathology Biorepository and Research Core > University of Maryland > Room NBW58, UMMC > 22 S. Greene St > Baltimore, MD 21201 > (410) 328-5524 > (410) 328-5508 fax > > > >>> "R C" 11/3/2008 12:49 pm >>> > Can someone assist me in rationalize the annual cost of maintining HT > certification (roughly $100 annually) and its benefit? Point accumulation > is > generally low for classes you must pay for, and those who obtained > certification prior to 2004 are exempt. Should one not pay the annual fee, > certification is dropped Is this correct?). In that case, can one advertise > "HT" certification for future employment opportunities then, offer full > explanation (and expired certification) during interview and that be > sufficient? > > What I generally receive from ASCP is an annual bill and a random > newsletter > from time to time. Furthermore, when a bill isn't paid on time, > the termingology in the subsequent bills become similar to that of a > collection agency. Frankly, I find this mailing submission as well as state > and national meetings more informative. > > Someone please clarify something I might be missing and any benefits of the > "pay out." > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This e-mail and any accompanying attachments may be privileged, > confidential, contain protected health information about an identified > patient or be otherwise protected from disclosure. State and federal law > protect the confidentiality of this information. If the reader of this > message is not the intended recipient; you are prohibited from using, > disclosing, reproducing or distributing this information; you should > immediately notify the sender by telephone or e-mail and delete this e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential > information intended only for the use of the recipient(s) named above. If > you are not the intended recipient, you may not print,distribute, or copy > this message or any attachments. If you have received this communication in > error, please notify the sender by return e-mail and delete this message and > any attachments from your computer. Any views or opinions expressed are > solely those of the author and do not necessarily represent those of > LRGHealthcare. > > ------------------------------ Message: 20 Date: Mon, 3 Nov 2008 20:09:48 -0500 From: "Chu, Shirley" Subject: [Histonet] Advances in LCM and Microgenomics Research Seminar To: Message-ID: Content-Type: text/plain; charset="US-ASCII" MDS Analytical Technologies will be hosting the following seminar at the Society for Neuroscience Meeting on Monday, November 12, 2008 Advances in LCM and Microgenomics Research Speakers include: * Charmaine Pietersen, Ph.D., McLean Hospital "Gene expression analysis in homogenous single cell populations in the superior temporal gyrus in postmortem brains from subjects with schizophrenia." * Jennifer A. Macdonald, Center for Vascular Biology and Department of Cell Biology, University of Connecticut Health Center "In situ analysis of blood-brain barrier gene expression using immuno-LCM coupled to qRT-PCR." For further information and to register for this seminar, please see the following website: http://www.moleculardevices.com/pages/lcm_at_neuroscience_11.2008.html Shirley Chu Application Scientist, Arcturus LCM Products Molecular Devices (now a part of MDS Analytical Technologies) 408-747-3765 | www.moleculardevices.com ------------------------------ Message: 21 Date: Tue, 4 Nov 2008 14:18:12 +1300 From: "Darren James" Subject: RE: [Histonet] Presidential Voting Infomation To: "'Tony Henwood'" , "'Jerry Helisek'" , Message-ID: Content-Type: text/plain; charset="us-ascii" .and we here in NZ have our own government elections this weekend....fun times all over the world :-) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Tuesday, 4 November 2008 11:06 a.m. To: Jerry Helisek; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Presidential Voting Infomation >From down here in Australia, we hope it goes well. We are thinking of you. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Helisek Sent: Tuesday, 4 November 2008 5:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Presidential Voting Infomation (Justin Thomas) This is just spam... Please delete this person. Thanks ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Tue, 4 Nov 2008 06:59:49 -0600 From: "Molinari, Betsy" Subject: [Histonet] wavy mid myocardium To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, I processed some mouse hearts that were in Z-Fix for 24 hrs. then put in 70% for anywhere from 24-48hrs. They were processed on a short run. The epicardium looks good but the mid myocardium is wavy. 15 min 80% 15 min 95 x 2 15 min 100% x 2 15 min Xylenes x2 10 min paraffin x 4 Any suggestions? Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) ------------------------------ Message: 23 Date: Tue, 4 Nov 2008 07:48:54 -0600 From: "Horn, Hazel V" Subject: RE: [Histonet] ASCP HT maintenance fees To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82EC2@EMAIL.archildrens.org> Content-Type: text/plain; charset="us-ascii" If you were certified before 2004, it doesn't matter if you pay your dues or not. You are still certified. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Abosso Sent: Monday, November 03, 2008 5:02 PM To: Kimberly Tuttle; R C; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP HT maintenance fees Kim, That is my understanding as well. Mary Abosso ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kimberly Tuttle Sent: Mon 11/3/2008 12:08 PM To: R C; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP HT maintenance fees Really? I never pay to maintain HT certification. As far as I know theres a ASCP membership fee, but you dont have to be a member to be certified. Am I wrong here? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax >>> "R C" 11/3/2008 12:49 pm >>> Can someone assist me in rationalize the annual cost of maintining HT certification (roughly $100 annually) and its benefit? Point accumulation is generally low for classes you must pay for, and those who obtained certification prior to 2004 are exempt. Should one not pay the annual fee, certification is dropped Is this correct?). In that case, can one advertise "HT" certification for future employment opportunities then, offer full explanation (and expired certification) during interview and that be sufficient? What I generally receive from ASCP is an annual bill and a random newsletter from time to time. Furthermore, when a bill isn't paid on time, the termingology in the subsequent bills become similar to that of a collection agency. Frankly, I find this mailing submission as well as state and national meetings more informative. Someone please clarify something I might be missing and any benefits of the "pay out." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 60, Issue 5 *************************************** THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From jgoldsmi <@t> bidmc.harvard.edu Tue Nov 4 10:28:21 2008 From: jgoldsmi <@t> bidmc.harvard.edu (jgoldsmi@bidmc.harvard.edu) Date: Tue Nov 4 10:28:40 2008 Subject: [Histonet] Political Post Message-ID: This is an inappropriate posting - we should only discuss relevant professional topics on this listserv. Jeff Goldsmith Boston, MA Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann Dunham of Wichita, Kansas. (white atheist ). When Obama was two years old, his parents divorced and his father returned to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta with Obama when he was six years old. Within six months he had learned to speak the Indonesian language. Obama spent "two years in a Muslim school, then two more in a Catholic school" in Jakarta. Obama takes great care to conceal the fact that he is a Muslim while admitting that he was once a Muslim, mitigating that damning information by saying that, for two years, he also attended a Catholic school. Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, Sr. and Dunham divorced when Barack, Jr. was two. Obama's spinmeisters are now attempting to make it appear that Obama's introduction to Islam came from his father and that influence was temporary at best. In reality, the senior Obama returned to Kenya immediately following the divorce and never again had any direct influence over his son's education. Dunham married another Muslim, Lolo Soetoro who educated his stepson as a good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism is the radical teaching that created the Muslim terrorists who are now waging Jihad on the industrialized world. Since it is politically expedient to be a Christian when you are seeking political office in the United States, Obama joined the United Church of Christ to help purge any notion that he is still a Muslim. Jeffrey Goldsmith, MD Department of Pathology Beth Israel Deaconess Medical Center 330 Brookline Avenue Boston, MA 02215 voice: 617 667 2586 FAX: 617 975 5620 From SAllen <@t> exchange.hsc.mb.ca Tue Nov 4 11:04:29 2008 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Tue Nov 4 11:04:37 2008 Subject: [Histonet] Question for "Lee & Peggy Wenk" about Mod. SDH Message-ID: Hi, I have a question for "Lee & Peggy Wenk" . I did a histosearch & came up with a question you had presented to the histonet about Modified SDH, (no date on it). I was wondering if you do this test and/or have any information on this test. We have been doing it since 1996 but need more technical information i.e. purpose of phenazine methosuphate & its limitations. Thanks for any help, Sharon Allen Neuropathology Laboratory Health Sciences Centre Winnipeg, MB, CA sallen@hsc.mb.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From LINDA.MARGRAF <@t> childrens.com Tue Nov 4 11:32:38 2008 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Tue Nov 4 11:40:39 2008 Subject: [Histonet] Presidential Voting Infomation etc. In-Reply-To: <685769.76562.qm@web35702.mail.mud.yahoo.com> References: <685769.76562.qm@web35702.mail.mud.yahoo.com> Message-ID: <491032D6.F783.00DA.0@childrens.com> Dear Histonetters: I am the list administrator and I would like to ask that people please refrain from further political comments on the list. I know this is a touchy topic but this is not the forum for these discussions. Please remember, my colleagues and I at the University of Texas Southwestern Medical Center run this list using University resources and it would be a real shame if they were to decide this was not a good use of their funds due to the off-topic comments etc. The Histonet list is an amazing collection of >3300 people from around the world helping each other and we really need to stick to topics pertinent to Histology. Thanks. Linda M Histonet administrator Linda Margraf, MD Professor of Pathology University of Texas Southwestern Medical School Dallas TX USA >>> Justin Thomas 11/3/2008 10:11 AM >>> Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE. THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please consider the environment before printing this e-mail

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From mcauliff <@t> umdnj.edu Tue Nov 4 09:09:41 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Nov 4 12:10:15 2008 Subject: [Histonet] wavy mid myocardium In-Reply-To: References: Message-ID: <491065B5.2050600@umdnj.edu> Hi Betsy: Wow, a real histology posting! If it is any comfort I have had the same problem with mouse heart after Zenker's fix (I wanted nice intercalated disks). I think the solution is to use NBF. Geoff Molinari, Betsy wrote: > Hi, > > I processed some mouse hearts that were in Z-Fix for 24 hrs. then put in > 70% for anywhere from 24-48hrs. They were processed on a short run. The > epicardium looks good but the mid myocardium is wavy. > > 15 min 80% > > 15 min 95 x 2 > > 15 min 100% x 2 > > 15 min Xylenes x2 > > 10 min paraffin x 4 > > Any suggestions? > > Thanks. > > > > > > Betsy Molinari HT(ASCP) > > Texas Heart Institute > > Cardiovascular Pathology > > 6770 Bertner Ave. > > MC 1-283 > > Houston, TX 77030 > > 832-355-6524 > > 832-355-6812 (fax) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From thomas.crowell <@t> novartis.com Tue Nov 4 12:10:27 2008 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Tue Nov 4 12:10:38 2008 Subject: [Histonet] FGF2 Antibody In-Reply-To: <200811041805.mA4I59wM009010@ch1ssaenov01.novartis.com> Message-ID: Dear Histonetters, I am looking for a mAb to FGF2 that works well in FFPE human tissues. Any recommendations? Regards, Thomas Crowell Novartis Institute for Biomedical Research Cambridge, MA From Dorothy.L.Webb <@t> HealthPartners.Com Tue Nov 4 12:21:17 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Nov 4 12:21:22 2008 Subject: [Histonet] recycling Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635AAA@hpes1.HealthPartners.int> We reycle xylene presently and are looking into recycling alcohol and formalin, mostly to look for a costs savings. For those of you in histoland that recycle those products, how generous are your cost savings? I am in doubt as to the fact that you cannot get 100% recycled alcohol as a byproduct of ones' recycling. I still will need to purchase absolute alcohol and that is the majority of alcohol ordered. Also, are the aldehydes and buffers costly that one needs to replenish in recycled formalin? And, has anyone seen any problems with their processing or staining when using these recycled products or do you find the same, optimal slide production?? Thanks much for sharing your experiences!! Dorothy Webb Regions Histology Technical Supervisor 651-254-2962 ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From Linda.Watson <@t> bms.com Tue Nov 4 12:30:32 2008 From: Linda.Watson <@t> bms.com (Linda M Watson) Date: Tue Nov 4 12:30:40 2008 Subject: [Histonet] re: Biocare Decloaker HIER In-Reply-To: <394A9DE849AA4B6B9661DF4CF0C6274E@auxs.umn.edu> References: <394A9DE849AA4B6B9661DF4CF0C6274E@auxs.umn.edu> Message-ID: <491094C8.2000808@bms.com> Hi Jan, We routinely use the Decloaker from Biocare. Normally, we use citrate buffer, pH=6.0 but that changes depending on tissue/antibody combination. I usually set the time around 1 minute, although I have gone as low at 30 seconds and as high as 3 minutes. The "set" temperature also varies depending on the number of containers I have. If I only have 1 or 2 plastic coplin jars then I set the temp at around 121 degrees C. If I have a large Tissue Tek container with approx 15-20 each then the temp needs to be at around 124.5 to 126 degrees C. The trick is to maintain the psi at around 18 _+_ 3 or so. Basically, there are no rules and you have to play with the parameters to obtain the optimal conditions for your assay. There are other folks in my lab area that set it for 15 minutes at around 95 degrees C, but the psi never goes above 5 or so. Sorry that I could not be more specific. For me, it really helps to understand the specificity of my antibody and the cellular expression / location. Good Luck, Linda Jan Shivers wrote: >For those of you who use Biocare's Decloaker for HIER on regular cases (not prion protein retrieval), could you tell me how long you do HIER with these machines? I normally do my prion cases in a Decloaker for 20', with a 25' cooldown, but I suspect that regular tissues don't need that length of time at high pressure/temp. I have an extra Decloaker and am considering switching to this device for my normal case work HIER, if it's time efficient. > >Please reply privately. Thanks. > >Jan Shivers >Senior Scientist >Histology/IHC/EM Section Head >Pathology Teaching Program >University of Minnesota >Veterinary Diagnostic Laboratory >1333 Gortner Ave. >St. Paul, MN 55108 >612-624-7297 >shive003@umn.edu >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Rcartun <@t> harthosp.org Tue Nov 4 12:32:53 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Nov 4 12:33:07 2008 Subject: [Histonet] Digital photography for gross pathology specimens Message-ID: <49104F050200007700006A41@gwmail4.harthosp.org> Can someone recommend a set-up for taking digital gross photos of pathology specimens in our frozen section room that doesn't take up a lot space, and doesn't cost "an arm and leg"? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From JWeems <@t> sjha.org Tue Nov 4 12:39:14 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Nov 4 12:39:49 2008 Subject: [Histonet] Digital photography for gross pathology specimens In-Reply-To: <49104F050200007700006A41@gwmail4.harthosp.org> References: <49104F050200007700006A41@gwmail4.harthosp.org> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA512710F@ITSSSXM01V6.one.ads.che.org> Try SPOT Imaging Solutions... www.spotimaging.com/pathstand 1-866-604-SPOT. We have in budget for next year.... good luck, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu ] On Behalf Of Richard Cartun Sent: Tuesday, November 04, 2008 1:33 PM To: Histonet Subject: [Histonet] Digital photography for gross pathology specimens Can someone recommend a set-up for taking digital gross photos of pathology specimens in our frozen section room that doesn't take up a lot space, and doesn't cost "an arm and leg"? Thank you. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From emerald_lake77 <@t> yahoo.com Tue Nov 4 12:45:04 2008 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Tue Nov 4 12:45:07 2008 Subject: [Histonet] Potential Job Opening for 2 Junior Level Histologists (Cambridge, MA) Message-ID: <681461.17259.qm@web31705.mail.mud.yahoo.com> POTENTIAL OPENING FOR TWO JUNIOR LEVEL HISTOLOGISTS CAMBRIDGE, MA AREA ? **Please forward this message to potential candidates** ? Seeking a motivated, team player that will bring his/her skills and experience to work in a progressive and dynamic environment - an effective communicator who is able to troubleshoot technical problems. ? -???????? Undergraduate degree required and ASCP certification (HT) preferred -???????? Basic histology skills and at least 1-2 years of practical experience in clinical or research needed -???????? Hands-on experience with small animal tissue dissection / necropsy desired -???????? Familiarity with fixation techniques, processing, embedding, sectioning and staining is essential -???????? Perform basic special stains and prepare solutions as needed -???????? Responsible for keeping inventory of tissues, blocks, and slides -???????? Ability to organize and prioritize tasks -???????? Reliability, conscientious with strong attention to detail -???????? Basic computer knowledge (data entry, MS Word / Excel) ? Other than general histology, the position will focus on computer analysis / morphometry as well as data entry and filing. ? The position(s) will cross train within various areas of the lab as well advanced techniques in immunofluorescence and general IHC. ? Please forward resumes ASAP and/or questions via EMAIL to: ? Gustave T. Hebert GHebert@wyeth.com ? Gustave Hebert Scientist II Wyeth Research Cambridge, MA From marktarango <@t> gmail.com Tue Nov 4 13:05:37 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Nov 4 13:05:42 2008 Subject: [Histonet] Presidential Voting Infomation etc. In-Reply-To: <491032D6.F783.00DA.0@childrens.com> References: <685769.76562.qm@web35702.mail.mud.yahoo.com> <491032D6.F783.00DA.0@childrens.com> Message-ID: <5b6eb13e0811041105n6198dc3l4cfd9b3dc5b07f62@mail.gmail.com> One day of talking politics doens't hurt anyone. ASCP is politically active isn't it? We can't talk about how it tries to influence politics? Saying that they'll close the histonet over this is ridiculous. Such important things need to be discussed. It affects histology and everthing and everyone else. Not talking leads to more problems. On 11/4/08, LINDA MARGRAF wrote: > > Dear Histonetters: > I am the list administrator and I would like to ask that people please > refrain from further political comments on the list. I know this is a touchy > topic but this is not the forum for these discussions. Please remember, my > colleagues and I at the University of Texas Southwestern Medical Center run > this list using University resources and it would be a real shame if they > were to decide this was not a good use of their funds due to the off-topic > comments etc. The Histonet list is an amazing collection of >3300 people > from around the world helping each other and we really need to stick to > topics pertinent to Histology. Thanks. > Linda M > Histonet administrator > > Linda Margraf, MD > Professor of Pathology > University of Texas Southwestern Medical School > Dallas TX USA > > > > > >>> Justin Thomas 11/3/2008 10:11 AM >>> > Barack Obama will raise taxes on hardworking Americans to give a government > handout to the 40% of Americans who pay no income taxes. > John McCain and Sarah Palin have an economic plan that celebrates the > American dream of opportunity, not government giveaways. In this country, we > believe in spreading opportunity, for those who need jobs and those who > create them. While Barack Obama is ready to "spread the wealth around," John > McCain has a plan to get our economy moving so everyone has access to good > jobs, a quality education and the opportunity to succeed. > > The next President won't have time to get used to the office. America faces > many challenges here at home, and many enemies abroad in this dangerous > world. We cannot spend the next four years as we have spent much of the last > eight: hoping for our luck to change at home and abroad. We need a new > direction, and John McCain and Sarah Palin will fight for it. > > Time and time again this team of mavericks has stood up, taken on tough > issues and delivered. They're the real deal. They have a clear record that > can deliver results, not just rhetoric that delivers votes. > > > PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK > ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE. THIS > IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST > DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Please consider the environment > before printing this e-mail
>
> > This e-mail, facsimile, or letter and > any files or attachments transmitted with it contains
> information that is confidential and privileged. This > information is intended only for the use of the
> individual(s) and entity(ies) to whom it is addressed. If > you are the intended recipient, further
> > disclosures are prohibited without proper authorization. If > you are not the intended recipient, any
> disclosure, copying, printing, or use of this information is > strictly prohibited and possibly a
> violation of federal or state law and regulations. If you > have received this information in error,
> please notify Children's Medical Center Dallas immediately > at 214-456-4444 or via e-mail at
> privacy@childrens.com. Children's Medical Center Dallas and > its affiliates hereby claim all
> applicable privileges related to this information.
/> > >
> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Barbara.Schormair <@t> helmholtz-muenchen.de Tue Nov 4 13:20:01 2008 From: Barbara.Schormair <@t> helmholtz-muenchen.de (Barbara Schormair) Date: Tue Nov 4 13:20:10 2008 Subject: [Histonet] Desperately seeking help for cryosectioning Message-ID: <4910A061.3080204@helmholtz-muenchen.de> Hi everybody, I'm in desperate need of some advice on cryosectioning of whole mouse embryos (age E11.5 up to E14.5). These are fixed in 4% PFA overnight at 4?C, then kept in 30% Sucrose until they sink to the bottom of the tube and then are transfered to molds filled with OTC. For freezing I cool down Isopentane on dry ice (15min prior to freezing the embryos), then I sink the molds in the isopentane for approx. 30 sec. After that I store them at -80?C. I've used the search option to see earlier postings on this topic, but they weren't really useful. I'm absolutely new to cryosectioning and unfortunately there is noone in my group who has any experience on this. I will give you guys a list of questions that I have (e.g. am I doing something wrong) and I would be really happy if someone could at least answer some of them. Thanks a lot in advance for your help. 1. Is something wrong with the fixation and freezing procedure, does this cause cracks and crumpling of my sections? 2. Is the whole embryo simply too heterogeneous in tissue texture, and does that cause cracks and crumpling? If so, should I decalcify or better dissect the embryo in e.g. brain, spinal cord and rest of the body with inner organs? 3. Which temperature is the best? I've tried from -16?C to -30?C. I also don't know how to change the temperature, when my sections start to a)crack or crumple b) coil up. 4. How do I prepare the OCT-block for sectioning. Steep or flat edges? How much OCT should I leave around the embryo for stabilizing the section? 5. When I start sectioning, the first sections are fine, after 15min they start to coil up or crumple, or stick together? Could this be caused by increased temperature in the sectioning chamber because it is not tightly closed anymore? How far does one close the chamber? Thanks a lot for any reply, I really need help on this. Are there by chance any tutorials or protocols for this available (not only outlining the procedures, but explaining why things sometimes don't work, and some troubleshooting). Or does someone know a good book on this topic. Best, Barbara From Barry.R.Rittman <@t> uth.tmc.edu Tue Nov 4 13:51:54 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Nov 4 13:51:59 2008 Subject: [Histonet] Presidential Voting Infomation etc. In-Reply-To: <5b6eb13e0811041105n6198dc3l4cfd9b3dc5b07f62@mail.gmail.com> References: <685769.76562.qm@web35702.mail.mud.yahoo.com> <491032D6.F783.00DA.0@childrens.com> <5b6eb13e0811041105n6198dc3l4cfd9b3dc5b07f62@mail.gmail.com> Message-ID: Mark you couldn't be more wrong. As Linda pointed out, such topics as discussing politics is regarded by most Universities as lobbying and as such is not permitted by University and State law because you are using University facilities. This could result in actions against the Universities involved especially in these times of cost cutting and very tight budgets. Universities that have state funding do not wish for their resources to be used for political purposes or for lobbying in general as this places their funding at risk. This could easily backlash when University budgets are being considered by the legislatures. You may recall a recent case when a church suggested that their members vote for a particular candidate. The IRS, appropriately so in my opinion, looked into revoking the charitable status of that church. ASCP is politically active as regards lobbying but (as far as I am aware) because of this does not enjoy tax exempt status. Let us keep Histonet as the professional well run, non political, service that it is. I would also suggest that if this situation arises in future that this email is not sent out to all, and as this is very difficult to monitor that, if it does get sent out that we do not reply to this as it just makes the situation worse (plus I already have a blister on my delete finger). Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Tuesday, November 04, 2008 1:06 PM To: LINDA MARGRAF Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Presidential Voting Infomation etc. One day of talking politics doens't hurt anyone. ASCP is politically active isn't it? We can't talk about how it tries to influence politics? Saying that they'll close the histonet over this is ridiculous. Such important things need to be discussed. It affects histology and everthing and everyone else. Not talking leads to more problems. On 11/4/08, LINDA MARGRAF wrote: > > Dear Histonetters: > I am the list administrator and I would like to ask that people please > refrain from further political comments on the list. I know this is a touchy > topic but this is not the forum for these discussions. Please remember, my > colleagues and I at the University of Texas Southwestern Medical Center run > this list using University resources and it would be a real shame if they > were to decide this was not a good use of their funds due to the off-topic > comments etc. The Histonet list is an amazing collection of >3300 people > from around the world helping each other and we really need to stick to > topics pertinent to Histology. Thanks. > Linda M > Histonet administrator > > Linda Margraf, MD > Professor of Pathology > University of Texas Southwestern Medical School > Dallas TX USA > > > > > >>> Justin Thomas 11/3/2008 10:11 AM >>> > Barack Obama will raise taxes on hardworking Americans to give a government > handout to the 40% of Americans who pay no income taxes. > John McCain and Sarah Palin have an economic plan that celebrates the > American dream of opportunity, not government giveaways. In this country, we > believe in spreading opportunity, for those who need jobs and those who > create them. While Barack Obama is ready to "spread the wealth around," John > McCain has a plan to get our economy moving so everyone has access to good > jobs, a quality education and the opportunity to succeed. > > The next President won't have time to get used to the office. America faces > many challenges here at home, and many enemies abroad in this dangerous > world. We cannot spend the next four years as we have spent much of the last > eight: hoping for our luck to change at home and abroad. We need a new > direction, and John McCain and Sarah Palin will fight for it. > > Time and time again this team of mavericks has stood up, taken on tough > issues and delivered. They're the real deal. They have a clear record that > can deliver results, not just rhetoric that delivers votes. > > > PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK > ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE. THIS > IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST > DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Please consider the environment > before printing this e-mail
>
> > This e-mail, facsimile, or letter and > any files or attachments transmitted with it contains
> information that is confidential and privileged. This > information is intended only for the use of the
> individual(s) and entity(ies) to whom it is addressed. If > you are the intended recipient, further
> > disclosures are prohibited without proper authorization. If > you are not the intended recipient, any
> disclosure, copying, printing, or use of this information is > strictly prohibited and possibly a
> violation of federal or state law and regulations. If you > have received this information in error,
> please notify Children's Medical Center Dallas immediately > at 214-456-4444 or via e-mail at
> privacy@childrens.com. Children's Medical Center Dallas and > its affiliates hereby claim all
> applicable privileges related to this information.
/> > >
> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Nov 4 14:24:14 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 4 14:24:18 2008 Subject: [Histonet] Her2/Neu Valadation In-Reply-To: Message-ID: <434532.52375.qm@web65716.mail.ac4.yahoo.com> Amy: The question has the answer in itself. You have to do the comparisons in the amount recommended between results in your lab vs. in other labs. That is what a validation requires. Ren? J. --- On Tue, 11/4/08, Amy Self wrote: From: Amy Self Subject: [Histonet] Her2/Neu Valadation To: histonet@lists.utsouthwestern.edu Date: Tuesday, November 4, 2008, 11:09 AM Dear Histonetters, How are you handling/answering the following question from the CAP checklist? Thanks in advance, Amy Georgetown Hospital System 843-527-7179 **NEW** 09/27/2007 ANP.22997 Phase I N/A YES NO If the laboratory assesses HER2 protein over-expression by immunohistochemistry (IHC) or HER2 gene amplification by fluorescence in situ hybridization (FISH), has the laboratory documented appropriate validation for the assay(s)? NOTE: Initial test validation must be performed on a minimum of 25 cases (recommended 25-100). Validation may be performed by comparing the results of testing with a validated alternative method (i.e., IHC vs. FISH) either in the same laboratory or another laboratory, or with the same validated method performed in another laboratory; validation testing must be done using the same set of cases in both labs. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Tue Nov 4 18:39:51 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Nov 4 18:39:56 2008 Subject: [Histonet] ASCP HT maintenance fees Message-ID: <582736990811041639g4da10444sc7dd18c32dd4b4b4@mail.gmail.com> Hi, I don't mean to be the fly in the ointment here, but in the words of Janet Jackson "What have you done for me lately?". It's a long standing joke among sticker collectors that aside from the certification the sticker is all you really get from the society. Where was the ASCP when NYS decided to pass that whacky law causing histotechs basically to have to be medtechs? The legislation seemed to actually take even them by surprise. There was little warning that this was going to happen. The main school for histology (SUNY Cobleskill) actually closed for a while after this. The proper thing for an advocate for histotechnology woule be to inform the profession of upcoming changes. Help those affected, and certainly make sure the educational requirements are fully lined up prior to the final passage of the laws. That is if they were paying attention. Where was ASCP? Worrying about medtechs perhaps. To my eye ASCP has much less interest in histotechnology (after certification) than histotechnology has in the ASCP. Just an observation, Amos Message: 12 Date: Tue, 4 Nov 2008 08:23:02 -0700 From: "Patsy Ruegg" Subject: RE: [Histonet] ASCP HT maintenance fees To: "'R C'" , "'Podawiltz, Thomas'" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" One of the benefits of ASCP membership is to support their efforts to fight for the medical technology profession in local and national legislation. With shortages, licensure, billing and all sorts of political issues that affect our lives we need someone to be working for us. CAP and CLIA does not do much for HT's since they still do not even require that a certified HT be doing the job. I get reports almost daily on what ASCP is doing for us and I support it by paying my membership dues. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org From slappycraw <@t> yahoo.com Tue Nov 4 19:30:52 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Tue Nov 4 19:30:55 2008 Subject: [Histonet] ASCP HT maintenance fees Message-ID: <143342.70960.qm@web53612.mail.re2.yahoo.com> Ditto on what Amos said. Histotechnology has been the ------ child of the laboratory forever. What other profession in medicine can you be on the street one day and the next be practicing histology in a lab? I've worked with people in the past that didn't know the difference between tissue and kleenex yet they were cutting sections in the lab and had name tags on saying they were histochnologists. Larry A. Woody Seattle, Wa. ________________________________ From: Amos Brooks To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, November 4, 2008 4:39:51 PM Subject: [Histonet] ASCP HT maintenance fees Hi, I don't mean to be the fly in the ointment here, but in the words of Janet Jackson "What have you done for me lately?". It's a long standing joke among sticker collectors that aside from the certification the sticker is all you really get from the society. Where was the ASCP when NYS decided to pass that whacky law causing histotechs basically to have to be medtechs? The legislation seemed to actually take even them by surprise. There was little warning that this was going to happen. The main school for histology (SUNY Cobleskill) actually closed for a while after this. The proper thing for an advocate for histotechnology woule be to inform the profession of upcoming changes. Help those affected, and certainly make sure the educational requirements are fully lined up prior to the final passage of the laws. That is if they were paying attention. Where was ASCP? Worrying about medtechs perhaps. To my eye ASCP has much less interest in histotechnology (after certification) than histotechnology has in the ASCP. Just an observation, Amos Message: 12 Date: Tue, 4 Nov 2008 08:23:02 -0700 From: "Patsy Ruegg" Subject: RE: [Histonet] ASCP HT maintenance fees To: "'R C'" , "'Podawiltz, Thomas'" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" One of the benefits of ASCP membership is to support their efforts to fight for the medical technology profession in local and national legislation. With shortages, licensure, billing and all sorts of political issues that affect our lives we need someone to be working for us. CAP and CLIA does not do much for HT's since they still do not even require that a certified HT be doing the job. I get reports almost daily on what ASCP is doing for us and I support it by paying my membership dues. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pieronelva01 <@t> bigpond.com Wed Nov 5 01:20:52 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Wed Nov 5 01:20:59 2008 Subject: [Histonet] re: Biocare Decloaker HIER References: <394A9DE849AA4B6B9661DF4CF0C6274E@auxs.umn.edu> Message-ID: I used it for 4 minutes at about 118 psi and most antibodies worked really well. I was having huge problems with the Er and Her2 and this solved them (almost) overnight. ----- Original Message ----- From: "Jan Shivers" To: "histonet" Sent: Wednesday, November 05, 2008 3:12 AM Subject: [Histonet] re: Biocare Decloaker HIER For those of you who use Biocare's Decloaker for HIER on regular cases (not prion protein retrieval), could you tell me how long you do HIER with these machines? I normally do my prion cases in a Decloaker for 20', with a 25' cooldown, but I suspect that regular tissues don't need that length of time at high pressure/temp. I have an extra Decloaker and am considering switching to this device for my normal case work HIER, if it's time efficient. Please reply privately. Thanks. Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.8.6/1765 - Release Date: 11/3/2008 4:59 PM From pieronelva01 <@t> bigpond.com Wed Nov 5 01:22:55 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Wed Nov 5 01:23:01 2008 Subject: [Histonet] Presidential Voting Infomation etc. References: <685769.76562.qm@web35702.mail.mud.yahoo.com> <491032D6.F783.00DA.0@childrens.com> Message-ID: <7843FE714590403DAF61C7AE60FBCA84@pentium4> A timely reminder. Great job on the Histonet in general, BTW. ----- Original Message ----- From: "LINDA MARGRAF" To: Sent: Wednesday, November 05, 2008 4:32 AM Subject: Re: [Histonet] Presidential Voting Infomation etc. Dear Histonetters: I am the list administrator and I would like to ask that people please refrain from further political comments on the list. I know this is a touchy topic but this is not the forum for these discussions. Please remember, my colleagues and I at the University of Texas Southwestern Medical Center run this list using University resources and it would be a real shame if they were to decide this was not a good use of their funds due to the off-topic comments etc. The Histonet list is an amazing collection of >3300 people from around the world helping each other and we really need to stick to topics pertinent to Histology. Thanks. Linda M Histonet administrator Linda Margraf, MD Professor of Pathology University of Texas Southwestern Medical School Dallas TX USA >>> Justin Thomas 11/3/2008 10:11 AM >>> Barack Obama will raise taxes on hardworking Americans to give a government handout to the 40% of Americans who pay no income taxes. John McCain and Sarah Palin have an economic plan that celebrates the American dream of opportunity, not government giveaways. In this country, we believe in spreading opportunity, for those who need jobs and those who create them. While Barack Obama is ready to "spread the wealth around," John McCain has a plan to get our economy moving so everyone has access to good jobs, a quality education and the opportunity to succeed. The next President won't have time to get used to the office. America faces many challenges here at home, and many enemies abroad in this dangerous world. We cannot spend the next four years as we have spent much of the last eight: hoping for our luck to change at home and abroad. We need a new direction, and John McCain and Sarah Palin will fight for it. Time and time again this team of mavericks has stood up, taken on tough issues and delivered. They're the real deal. They have a clear record that can deliver results, not just rhetoric that delivers votes. PLEASE GIVE CAREFUL THOUGHT WHO YOU WILL VOTE FOR ON TUESDAY...PLEASE THINK ABOUT WHERE THESE TWO MEN COME FROM AND WHERE THEIR HEARTS TRULY ARE. THIS IS A CRUTIAL ELECTION AND WE AS AMERICANS MUST BE CONFIDENT THAT OUR BEST DAYS ARE AHEAD OF US WITH JOHN LEADING THE WAY!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please consider the environment before printing this e-mail

This e-mail, facsimile, or letter and any files or attachments transmitted with it contains
information that is confidential and privileged. This information is intended only for the use of the
individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further
disclosures are prohibited without proper authorization. If you are not the intended recipient, any
disclosure, copying, printing, or use of this information is strictly prohibited and possibly a
violation of federal or state law and regulations. If you have received this information in error,
please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at
privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all
applicable privileges related to this information.

_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.8.6/1765 - Release Date: 11/3/2008 4:59 PM From sam.histology <@t> gmail.com Wed Nov 5 00:56:31 2008 From: sam.histology <@t> gmail.com (Sam Histology) Date: Wed Nov 5 01:57:19 2008 Subject: [Histonet] WOE TO AMERICA! Message-ID: http://www.youtube.com/watch?v=wFYk7a3vEyo I don't know how many times I've heard the words "Obama" and "Messiah" used in the same sentence. To liberals, Barack Obama is a spotless god-like figure who will save America from the scourge of neo-conservatism. I'm totally sick of hearing and reading your hero-worshiping crap, folks. Let's face reality: *like so many other powerful politicians in this country, Barack Obama sucks*: 1. *The Race Card*: Whether it be in suggesting that anyone who doesn't vote for him because he is black is probably a republican, or in blaming Bush administration racismon a slow response to Hurricane Katrina, Obama is quite comfortable playing the race card. 2. *Anti-Indian*: After the Obama campaign released a paper disparaging other candidates for their ties to the Indian-American community, the chairman of the bipartisan US India Political Action Committee, Sanjay Puri, stated that the Obama Campaign was "engaging in the worst kind of anti-Indian American stereotyping." Of course, Obama denied any hand in the racist document put out by his campaign. 3. *Corrupt Buddies*: Tony Rezko, a long time friend and fund-raiser for Obama, was indicted last fall on federal charges that accuse him of demanding kickbacks from companies seeking state business. When asked about his friend, Obama said, "I've never done any favors for him." This turned out to be a lie, as evidence turned upproving that Obama had written letters to city and state officials praising Rezko's business practices. 4. *Wal-Mart Ties*: While bashing of Wal-Mart's labor practices in public, Obama has been profiting from their businessthrough the money his wife made as a member of the board of directors for a company that produces food for the mega-corporation. 5. *Religious Ties*: Is Obama a Muslim? Is he a Christian? Nobody is 100% sure, but it is true that Obama was raised in a Muslim family and at one time attended an Islamic school. He currently claims to be a convert to Christianity, but some are concerned about his Muslim upbringing . 6. *Anti-Second Amendment*: Obama is one of the most anti-Second Amendment legislators in the country. He supports a ban the sale or transfer of *all* forms of semi-automatic weapons. 7. *Gas-guzzler*: Obama might attack American automakers for not making enough environmental friendly automobiles, but when he goes home he drives a gas-guzzling V-8 hemi-powered Chrysler 300 . 8. *Obama Ringtones*: The most annoying campaign tool ever . 9. *Obama Girl*: I take back what I said about the ringtones. This girlis far more annoying. 10. *His Unelectable Name*: Barack Hussein Obama, 'nuff said. On Tue, Nov 4, 2008 at 6:47 PM, Linda Jones wrote: > > stupid!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! > > Linda Harper-Jones BS.,HT/ HTL(ASCP) > University of Mississippi Medical Center > Department of Pathology > Chief Histotechnologist Supervisor > Telephone (601) 984-1576 > Fax (601) 984-4968 > ljones@pathology.umsmed.edu > > > The information contained in this email is confidential. Individuals who > have received this information in error or are not authorized to receive it > must promptly return or dispose of the information and notify the sender. > Those individuals are hereby notified that they are strictly prohibited > from reviewing, forwarding, printing, copying, distributing or using this > information in any way. > > > >>> "Sam Histology" 11/04/08 8:48 AM >>> > Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama > Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann > Dunham > of Wichita, Kansas. (white atheist ). > > When Obama was two years old, his parents divorced and his father returned > to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta > with Obama when he was six years old. Within six months he had learned to > speak the Indonesian language. Obama spent "two years in a Muslim school, > then two more in a Catholic school" in Jakarta. Obama takes great care to > conceal the fact that he is a Muslim while admitting that he was once a > Muslim, mitigating that damning information by saying that, for two years, > he also attended a Catholic school. > > Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated > from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white > atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, > Sr. and Dunham divorced when Barack, Jr. was two. > > Obama's spinmeisters are now attempting to make it appear that Obama's > introduction to Islam came from his father and that influence was temporary > at best. > > In reality, the senior Obama returned to Kenya immediately following the > divorce and never again had any direct influence over his son's education. > > Dunham married another Muslim, Lolo Soetoro who educated his stepson as a > good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism > is the radical teaching that created the Muslim terrorists who are now > waging Jihad on the industrialized world. > > Since it is politically expedient to be a Christian when you are seeking > political office in the United States, Obama joined the United Church of > Christ to help purge any notion that he is still a Muslim. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Individuals who have received this information in error or are not > authorized to receive it must promptly return or dispose of the information > and notify the sender. Those individuals are hereby notified that they are > strictly prohibited from reviewing, forwarding, printing, copying, > distributing or using this information in any way. > > > From Linda.Watson <@t> bms.com Wed Nov 5 06:07:26 2008 From: Linda.Watson <@t> bms.com (Linda M Watson) Date: Wed Nov 5 06:07:33 2008 Subject: [Histonet] re: Biocare Decloaker HIER In-Reply-To: References: <394A9DE849AA4B6B9661DF4CF0C6274E@auxs.umn.edu> Message-ID: <49118C7E.7000207@bms.com> Is the 118 psi a typo? I don't believe the instrument can go that high-max psi is 30. Piero Nelva wrote: > I used it for 4 minutes at about 118 psi and most antibodies worked > really well. I was having huge problems with the Er and Her2 and this > solved them (almost) overnight. > > > ----- Original Message ----- From: "Jan Shivers" > To: "histonet" > Sent: Wednesday, November 05, 2008 3:12 AM > Subject: [Histonet] re: Biocare Decloaker HIER > > > For those of you who use Biocare's Decloaker for HIER on regular cases > (not prion protein retrieval), could you tell me how long you do HIER > with these machines? I normally do my prion cases in a Decloaker for > 20', with a 25' cooldown, but I suspect that regular tissues don't > need that length of time at high pressure/temp. I have an extra > Decloaker and am considering switching to this device for my normal > case work HIER, if it's time efficient. > > Please reply privately. Thanks. > > Jan Shivers > Senior Scientist > Histology/IHC/EM Section Head > Pathology Teaching Program > University of Minnesota > Veterinary Diagnostic Laboratory > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -------------------------------------------------------------------------------- > > > > > No virus found in this incoming message. > Checked by AVG - http://www.avg.com > Version: 8.0.175 / Virus Database: 270.8.6/1765 - Release Date: > 11/3/2008 4:59 PM > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Wed Nov 5 06:23:01 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Nov 5 06:23:06 2008 Subject: [Histonet] WOE TO AMERICA! In-Reply-To: References: Message-ID: Isn't the election over? Griping will do you no good, spambot! So, being in grant funded research, I hope our new president will put some money back into NIH. (I was hoping that no matter who won, so my political views will not spam you) Anyone else feel that way? Emily -- "You would know her for all the things she was...a woman who knew her way in and out of every new book without being singed, pinched, bumped or tickled by any line or chapter." John O'Hara, Appointment in Samarra From mcauliff <@t> umdnj.edu Wed Nov 5 08:24:19 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Nov 5 08:42:48 2008 Subject: [Histonet] funding for NIH In-Reply-To: References: Message-ID: <4911AC93.9080209@umdnj.edu> Hi Emily et al. The President, any President, can put $ for NIH is the budget request he/she submits to Congress but ONLY Congress can appropriate money. That is in the Constitution. Several of my colleagues are out of work due to loss of grant support 'cause NIH, while not broke, has many worthy projects to fund and cannot fund them all. Geoff Emily Sours wrote: > Isn't the election over? > Griping will do you no good, spambot! > > So, being in grant funded research, I hope our new president will put some > money back into NIH. > (I was hoping that no matter who won, so my political views will not spam > you) > Anyone else feel that way? > > Emily > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Phyllis_Burnette <@t> bshsi.org Wed Nov 5 08:45:53 2008 From: Phyllis_Burnette <@t> bshsi.org (Burnette, Phyllis) Date: Wed Nov 5 08:45:53 2008 Subject: [Histonet] ASCP HT maintenance fees In-Reply-To: <2a926e3f0811031528x60d57f15q9556368cc557f48b@mail.gmail.com> References: <2a926e3f0811030949g8a5ac14q37bb30d3ac648957@mail.gmail.com><490F05CA.90CE.001A.3@umm.edu><38667E7FB77ECD4E91BFAEB8D98638631D2D14DB3D@LRGHEXVS1.practice.lrgh.org> <2a926e3f0811031528x60d57f15q9556368cc557f48b@mail.gmail.com> Message-ID: <2C7750460C3E7545AC11099BE6A7ABD601C993F0@EDC-MAIL-02.ads.bshsi.com> Sorry RC, Now who's being biased and where is the fairness? You imply older histotechs do not study hard and receive a salary that they may or may not be entitled to?? I go to college and take night classes to keep from being sterotyped this way. I also have staff that I would stand up for hands down (certified or non-certified) vs someone that just got out of school, has a lot of textbook knowledge, and is certified. The heathcare industry regarding Histology is just now becoming acutely aware of the impact the new changes have made. We as Histotechs need to stand together and support one another (certified or non-certified)through this difficult and stressful time in the workplace. Thanks Phyllis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R C Sent: Monday, November 03, 2008 6:29 PM To: Podawiltz, Thomas Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP HT maintenance fees Thanks for the many replies however my objective hasn't been addressed. For the supervisors; My point is not to mislead and claim ASCP certification without having it. My question is to assist in rationalizing cost. Disregarding a credible applicant who doesn't see the financial investment towards ASCP shouldn't be interpreted as not being serious about a field. That ideology promotes "pay to play." It's a direct question towards ASCP membership feedback and prejudice (pre-2004 certification). As far as seriousness of the field, revert to my original objective of understanding what my dues fund, what do I as a tech directly receive in return, and why are older tech's exempt? The truth is many techs employed in clinical labs who do not have certification receive comparable salaries to those histotechs who study hard, pass the HT exam, and pay ASCP dues. Where is the justification in that? How does JACHO feel about that? I pose a credible question of fairness and return investment. Cash is king now and where I spend it is ever important. On Mon, Nov 3, 2008 at 12:06 PM, Podawiltz, Thomas wrote: > If you were certified in 2004 or after you need to turn in 36 credit > hours of continuing education in order to maintain your certification. > You do not need to be a member of ASCP to be certified, however you do > get some free CE hours with your membership. My certification was in > 85, so yes, I am one of the old farts that is exempt. However, I have > stayed current with my education. even in the years that I did not practice Histology. > > As a supervisor, I would not look at a resume that had an expired > certification. Right or wrong I would assume that, the applicant did > not take this field seriously enough by letting their certification lapse. > > Tom Podawiltz, HT (ASCP) > Histology Section Head/Laboratory Safety Officer LRGHealthcare > 603-524-3211 ext: 3220 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly > Tuttle [ ktuttle@umm.edu] > Sent: Monday, November 03, 2008 2:08 PM > To: R C; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] ASCP HT maintenance fees > > Really? I never pay to maintain HT certification. As far as I know > theres a ASCP membership fee, but you dont have to be a member to be > certified. Am I wrong here? > > Kimberly C. Tuttle HT (ASCP) > Pathology Biorepository and Research Core University of Maryland Room > NBW58, UMMC > 22 S. Greene St > Baltimore, MD 21201 > (410) 328-5524 > (410) 328-5508 fax > > > >>> "R C" 11/3/2008 12:49 pm >>> > Can someone assist me in rationalize the annual cost of maintining HT > certification (roughly $100 annually) and its benefit? Point > accumulation is generally low for classes you must pay for, and those > who obtained certification prior to 2004 are exempt. Should one not > pay the annual fee, certification is dropped Is this correct?). In > that case, can one advertise "HT" certification for future employment > opportunities then, offer full explanation (and expired certification) > during interview and that be sufficient? > > What I generally receive from ASCP is an annual bill and a random > newsletter from time to time. Furthermore, when a bill isn't paid on > time, the termingology in the subsequent bills become similar to that > of a collection agency. Frankly, I find this mailing submission as > well as state and national meetings more informative. > > Someone please clarify something I might be missing and any benefits > of the "pay out." > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This e-mail and any accompanying attachments may be privileged, > confidential, contain protected health information about an identified > patient or be otherwise protected from disclosure. State and federal > law protect the confidentiality of this information. If the reader of > this message is not the intended recipient; you are prohibited from > using, disclosing, reproducing or distributing this information; you > should immediately notify the sender by telephone or e-mail and delete this e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and > confidential information intended only for the use of the recipient(s) > named above. If you are not the intended recipient, you may not > print,distribute, or copy this message or any attachments. If you > have received this communication in error, please notify the sender by > return e-mail and delete this message and any attachments from your > computer. Any views or opinions expressed are solely those of the > author and do not necessarily represent those of LRGHealthcare. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From kadamsplw <@t> gmail.com Wed Nov 5 09:10:51 2008 From: kadamsplw <@t> gmail.com (karen adams) Date: Wed Nov 5 09:10:59 2008 Subject: [Histonet] prostate core processing Message-ID: Greetings, Can anyone offer a processing schedual for prostate bx cores for the Leica processor?? From HACKERLAB <@t> aol.com Wed Nov 5 09:54:32 2008 From: HACKERLAB <@t> aol.com (HACKERLAB@aol.com) Date: Wed Nov 5 09:54:46 2008 Subject: [Histonet] Thank you! Message-ID: Many thanks for intervening. Elfi Hacker Hacker Industries Winnsboro, SC Dear Histonetters: I am the list administrator and I would like to ask that people please refrain from further political comments on the list. I know this is a touchy topic but this is not the forum for these discussions. Please remember, my colleagues and I at the University of Texas Southwestern Medical Center run this list using University resources and it would be a real shame if they were to decide this was not a good use of their funds due to the off-topic comments etc. The Histonet list is an amazing collection of >3300 people from around the world helping each other and we really need to stick to topics pertinent to Histology. Thanks. Linda M Histonet administrator Linda Margraf, MD Professor of Pathology University of Texas Southwestern Medical School Dallas TX USA **************AOL Search: Your one stop for directions, recipes and all other Holiday needs. Search Now. (http://pr.atwola.com/promoclk/100000075x1212792382x1200798498/aol?redir=http://searchblog.aol.com/2008/11/04/happy-holidays-from -aol-search/?ncid=emlcntussear00000001) From joachim.hehl <@t> lmc.biol.ethz.ch Wed Nov 5 09:58:03 2008 From: joachim.hehl <@t> lmc.biol.ethz.ch (Joachim Hehl) Date: Wed Nov 5 09:58:09 2008 Subject: [Histonet] President Message-ID: Interesting, the histonet list seems to be occupied by ultraconservatives. From Gina.Rodriguez <@t> leica-microsystems.com Wed Nov 5 10:00:57 2008 From: Gina.Rodriguez <@t> leica-microsystems.com (Gina.Rodriguez@leica-microsystems.com) Date: Wed Nov 5 10:04:14 2008 Subject: [Histonet] Gina Rodriguez is out of the office. Message-ID: I will be out of the office starting 11/05/2008 and will not return until 11/10/2008. I will respond to your message when I return. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From HACKERLAB <@t> aol.com Wed Nov 5 09:52:25 2008 From: HACKERLAB <@t> aol.com (HACKERLAB@aol.com) Date: Wed Nov 5 10:25:08 2008 Subject: [Histonet] What the heck is this doiing on the HistoNet??? Message-ID: This kind of material really should not be posted on the HistoNet! Why don't you find some other place to vent! Elfi Hacker Hacker Industries Inc http://www.youtube.com/watch?v=wFYk7a3vEyo I don't know how many times I've heard the words "Obama" and "Messiah" used in the same sentence. To liberals, Barack Obama is a spotless god-like figure who will save America from the scourge of neo-conservatism. I'm totally sick of hearing and reading your hero-worshiping crap, folks. Let's face reality: *like so many other powerful politicians in this country, Barack Obama sucks*: 1. *The Race Card*: Whether it be in suggesting that anyone who doesn't vote for him because he is black is probably a republican, or in blaming Bush administration racismon a slow response to Hurricane Katrina, Obama is quite comfortable playing the race card. 2. *Anti-Indian*: After the Obama campaign released a paper disparaging other candidates for their ties to the Indian-American community, the chairman of the bipartisan US India Political Action Committee, Sanjay Puri, stated that the Obama Campaign was "engaging in the worst kind of anti-Indian American stereotyping." Of course, Obama denied any hand in the racist document put out by his campaign. 3. *Corrupt Buddies*: Tony Rezko, a long time friend and fund-raiser for Obama, was indicted last fall on federal charges that accuse him of demanding kickbacks from companies seeking state business. When asked about his friend, Obama said, "I've never done any favors for him." This turned out to be a lie, as evidence turned upprovin g that Obama had written letters to city and state officials praising Rezko's business practices. 4. *Wal-Mart Ties*: While bashing of Wal-Mart's labor practices in public, Obama has been profiting from their businessthrough the money his wife made as a member of the board of directors for a company that produces food for the mega-corporation. 5. *Religious Ties*: Is Obama a Muslim? Is he a Christian? Nobody is 100% sure, but it is true that Obama was raised in a Muslim family and at one time attended an Islamic school. He currently claims to be a convert to Christianity, but some are concerned about his Muslim upbringing . 6. *Anti-Second Amendment*: Obama is one of the most anti-Second Amendment legislators in the country. He supports a ban the sale or transfer of *all* forms of semi-automatic weapons. 7. *Gas-guzzler*: Obama might attack American automakers for not making enough environmental friendly automobiles, but when he goes home he drives a gas-guzzling V-8 hemi-powered Chrysler 300 . 8. *Obama Ringtones*: The most annoying campaign tool ever . 9. *Obama Girl*: I take back what I said about the ringtones. This girlis far more annoying. 10. *His Unelectable Name*: Barack Hussein Obama, 'nuff said. On Tue, Nov 4, 2008 at 6:47 PM, Linda Jones wrote: > > stupid!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! > > Linda Harper-Jones BS.,HT/ HTL(ASCP) > University of Mississippi Medical Center > Department of Pathology > Chief Histotechnologist Supervisor > Telephone (601) 984-1576 > Fax (601) 984-4968 > ljones@pathology.umsmed.edu > > > The information contained in this email is confidential. Individuals who > have received this information in error or are not authorized to receive it > must promptly return or dispose of the information and notify the sender. > Those individuals are hereby notified that they are strictly prohibited > from reviewing, forwarding, printing, copying, distributing or using this > information in any way. > > > >>> "Sam Histology" 11/04/08 8:48 AM >>> > Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama > Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann > Dunham > of Wichita, Kansas. (white atheist ). > > When Obama was two years old, his parents divorced and his father returned > to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta > with Obama when he was six years old. Within six months he had learned to > speak the Indonesian language. Obama spent "two years in a Muslim school, > then two more in a Catholic school" in Jakarta. Obama takes great care to > conceal the fact that he is a Muslim while admitting that he was once a > Muslim, mitigating that damning information by saying that, for two years, > he also attended a Catholic school. > > Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated > from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white > atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, > Sr. and Dunham divorced when Barack, Jr. was two. > > Obama's spinmeisters are now attempting to make it appear that Obama's > introduction to Islam came from his father and that influence was temporary > at best. > > In reality, the senior Obama returned to Kenya immediately following the > divorce and never again had any direct influence over his son's education. > > Dunham married another Muslim, Lolo Soetoro who educated his stepson as a > good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism > is the radical teaching that created the Muslim terrorists who are now > waging Jihad on the industrialized world. > > Since it is politically expedient to be a Christian when you are seeking > political office in the United States, Obama joined the United Church of > Christ to help purge any notion that he is still a Muslim. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Individuals who have received this information in error or are not > authorized to receive it must promptly return or dispose of the information > and notify the sender. Those individuals are hereby notified that they are > strictly prohibited from reviewing, forwarding, printing, copying, > distributing or using this information in any way. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************AOL Search: Your one stop for directions, recipes and all other Holiday needs. Search Now. (http://pr.atwola.com/promoclk/100000075x1212792382x1200798498/aol?redir=http://searchblog.aol.com/2008/11/04/happy-holidays-from -aol-search/?ncid=emlcntussear00000001) From mickie25 <@t> netzero.net Wed Nov 5 10:28:31 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Nov 5 10:28:44 2008 Subject: [Histonet] What the heck is this doiing on the HistoNet??? In-Reply-To: References: Message-ID: Ditto! Best Regards, ? Mickie ? Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC ? & Mohs Lab Staffing & Mohs Suite Mohs Reporting Software 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX?? 509-624-3926 Web: www.mohshistogyconsulting.com?& www.mohslabstaffing.com & www.mohssuite.com Email: mickie25@netzero.net ? DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. Thank You. ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of HACKERLAB@aol.com Sent: Wednesday, November 05, 2008 7:52 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What the heck is this doiing on the HistoNet??? This kind of material really should not be posted on the HistoNet! Why don't you find some other place to vent! Elfi Hacker Hacker Industries Inc http://www.youtube.com/watch?v=wFYk7a3vEyo I don't know how many times I've heard the words "Obama" and "Messiah" used in the same sentence. To liberals, Barack Obama is a spotless god-like figure who will save America from the scourge of neo-conservatism. I'm totally sick of hearing and reading your hero-worshiping crap, folks. Let's face reality: *like so many other powerful politicians in this country, Barack Obama sucks*: 1. *The Race Card*: Whether it be in suggesting that anyone who doesn't vote for him because he is black is probably a republican, or in blaming Bush administration racismon a slow response to Hurricane Katrina, Obama is quite comfortable playing the race card. 2. *Anti-Indian*: After the Obama campaign released a paper disparaging other candidates for their ties to the Indian-American community, the chairman of the bipartisan US India Political Action Committee, Sanjay Puri, stated that the Obama Campaign was "engaging in the worst kind of anti-Indian American stereotyping." Of course, Obama denied any hand in the racist document put out by his campaign. 3. *Corrupt Buddies*: Tony Rezko, a long time friend and fund-raiser for Obama, was indicted last fall on federal charges that accuse him of demanding kickbacks from companies seeking state business. When asked about his friend, Obama said, "I've never done any favors for him." This turned out to be a lie, as evidence turned upprov in g that Obama had written letters to city and state officials praising Rezko's business practices. 4. *Wal-Mart Ties*: While bashing of Wal-Mart's labor practices in public, Obama has been profiting from their businessthrough the money his wife made as a member of the board of directors for a company that produces food for the mega-corporation. 5. *Religious Ties*: Is Obama a Muslim? Is he a Christian? Nobody is 100% sure, but it is true that Obama was raised in a Muslim family and at one time attended an Islamic school. He currently claims to be a convert to Christianity, but some are concerned about his Muslim upbringing . 6. *Anti-Second Amendment*: Obama is one of the most anti-Second Amendment legislators in the country. He supports a ban the sale or transfer of *all* forms of semi-automatic weapons. 7. *Gas-guzzler*: Obama might attack American automakers for not making enough environmental friendly automobiles, but when he goes home he drives a gas-guzzling V-8 hemi-powered Chrysler 300 . 8. *Obama Ringtones*: The most annoying campaign tool ever . 9. *Obama Girl*: I take back what I said about the ringtones. This girlis far more annoying. 10. *His Unelectable Name*: Barack Hussein Obama, 'nuff said. On Tue, Nov 4, 2008 at 6:47 PM, Linda Jones wrote: > > stupid!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! > > Linda Harper-Jones BS.,HT/ HTL(ASCP) > University of Mississippi Medical Center > Department of Pathology > Chief Histotechnologist Supervisor > Telephone (601) 984-1576 > Fax (601) 984-4968 > ljones@pathology.umsmed.edu > > > The information contained in this email is confidential. Individuals who > have received this information in error or are not authorized to receive it > must promptly return or dispose of the information and notify the sender. > Those individuals are hereby notified that they are strictly prohibited > from reviewing, forwarding, printing, copying, distributing or using this > information in any way. > > > >>> "Sam Histology" 11/04/08 8:48 AM >>> > Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama > Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann > Dunham > of Wichita, Kansas. (white atheist ). > > When Obama was two years old, his parents divorced and his father returned > to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta > with Obama when he was six years old. Within six months he had learned to > speak the Indonesian language. Obama spent "two years in a Muslim school, > then two more in a Catholic school" in Jakarta. Obama takes great care to > conceal the fact that he is a Muslim while admitting that he was once a > Muslim, mitigating that damning information by saying that, for two years, > he also attended a Catholic school. > > Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated > from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white > atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, > Sr. and Dunham divorced when Barack, Jr. was two. > > Obama's spinmeisters are now attempting to make it appear that Obama's > introduction to Islam came from his father and that influence was temporary > at best. > > In reality, the senior Obama returned to Kenya immediately following the > divorce and never again had any direct influence over his son's education. > > Dunham married another Muslim, Lolo Soetoro who educated his stepson as a > good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism > is the radical teaching that created the Muslim terrorists who are now > waging Jihad on the industrialized world. > > Since it is politically expedient to be a Christian when you are seeking > political office in the United States, Obama joined the United Church of > Christ to help purge any notion that he is still a Muslim. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Individuals who have received this information in error or are not > authorized to receive it must promptly return or dispose of the information > and notify the sender. Those individuals are hereby notified that they are > strictly prohibited from reviewing, forwarding, printing, copying, > distributing or using this information in any way. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************AOL Search: Your one stop for directions, recipes and all other Holiday needs. Search Now. (http://pr.atwola.com/promoclk/100000075x1212792382x1200798498/aol?redir=htt p://searchblog.aol.com/2008/11/04/happy-holidays-from -aol-search/?ncid=emlcntussear00000001) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.8.5/1763 - Release Date: 11/2/2008 7:08 PM From c.weaver <@t> vla.defra.gsi.gov.uk Wed Nov 5 11:02:37 2008 From: c.weaver <@t> vla.defra.gsi.gov.uk (Weaver, Colin) Date: Wed Nov 5 11:02:45 2008 Subject: [Histonet] Fungal staining Message-ID: <7A885E8FE1C71C488D974EC601FAA690041C23C6@vla-exchn1.cvlnt.vla.gov.uk> Hi - everyone - does anyone know of any fungi that can not be demonstrated by PAS stain? We appear to have one in a cow placenta which is obvious on Grocott and H&E but do not stain on PAS. The micro lab isolated Absidia spp but the hyphae in the Grocott are septate but I believe Absidia are aseptate. Any ideas would be most welcome. Colin Weaver VLA Thirsk England Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. From marktarango <@t> gmail.com Wed Nov 5 11:16:43 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Nov 5 11:16:47 2008 Subject: [Histonet] funding for NIH In-Reply-To: <4911AC93.9080209@umdnj.edu> References: <4911AC93.9080209@umdnj.edu> Message-ID: <5b6eb13e0811050916o74c6269ei1fb9fd0a91bfc79@mail.gmail.com> I definately hope that now that Obama is going to be President that the reseach will get moving again. He's got The Congress too. P.S. I hope this message doesn't shut the histonet down. Mark On 11/5/08, Geoff McAuliffe wrote: > > Hi Emily et al. > > The President, any President, can put $ for NIH is the budget request > he/she submits to Congress but ONLY Congress can appropriate money. That is > in the Constitution. > Several of my colleagues are out of work due to loss of grant support > 'cause NIH, while not broke, has many worthy projects to fund and cannot > fund them all. > > Geoff > > Emily Sours wrote: > >> Isn't the election over? >> Griping will do you no good, spambot! >> >> So, being in grant funded research, I hope our new president will put some >> money back into NIH. >> (I was hoping that no matter who won, so my political views will not spam >> you) >> Anyone else feel that way? >> >> Emily >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From slappycraw <@t> yahoo.com Wed Nov 5 11:17:39 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Wed Nov 5 11:17:43 2008 Subject: [Histonet] Fungal staining References: <7A885E8FE1C71C488D974EC601FAA690041C23C6@vla-exchn1.cvlnt.vla.gov.uk> Message-ID: <646095.66619.qm@web53607.mail.re2.yahoo.com> Maybe Aspergillus or Rhizopus. ? Larry A. Woody Seattle, Wa. ________________________________ From: "Weaver, Colin" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 5, 2008 9:02:37 AM Subject: [Histonet] Fungal staining Hi - everyone - does anyone know of any fungi that can not be demonstrated by PAS stain? We appear to have one in a cow placenta which is obvious on Grocott and H&E but do not stain on PAS. The micro lab isolated Absidia spp but the hyphae in the Grocott are septate but I believe Absidia are aseptate. Any ideas would be most welcome. Colin Weaver VLA Thirsk England ? Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Nov 5 11:18:21 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Nov 5 11:18:41 2008 Subject: AW: [Histonet] Fungal staining In-Reply-To: <7A885E8FE1C71C488D974EC601FAA690041C23C6@vla-exchn1.cvlnt.vla.gov.uk> References: <7A885E8FE1C71C488D974EC601FAA690041C23C6@vla-exchn1.cvlnt.vla.gov.uk> Message-ID: <34E7498B0F784743A2AFE5C210716047@dielangs.at> Try a CAS stain. = PAS with Chromic acid Perhaps this fungus needs something stronger than Periodic acid. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Weaver, Colin Gesendet: Mittwoch, 05. November 2008 18:03 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Fungal staining Hi - everyone - does anyone know of any fungi that can not be demonstrated by PAS stain? We appear to have one in a cow placenta which is obvious on Grocott and H&E but do not stain on PAS. The micro lab isolated Absidia spp but the hyphae in the Grocott are septate but I believe Absidia are aseptate. Any ideas would be most welcome. Colin Weaver VLA Thirsk England Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Nov 5 11:38:13 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Nov 5 11:38:26 2008 Subject: [Histonet] funding for NIH In-Reply-To: <5b6eb13e0811050916o74c6269ei1fb9fd0a91bfc79@mail.gmail.com> References: <4911AC93.9080209@umdnj.edu> <5b6eb13e0811050916o74c6269ei1fb9fd0a91bfc79@mail.gmail.com> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C46E@LTA3VS011.ees.hhs.gov> All I will say on the matter is that if more funding is approved I hope it is simply pulled from somewhere else and is not in addition to.....because that would mean more taxes! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Wednesday, November 05, 2008 12:17 PM To: Geoff McAuliffe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] funding for NIH I definately hope that now that Obama is going to be President that the reseach will get moving again. He's got The Congress too. P.S. I hope this message doesn't shut the histonet down. Mark On 11/5/08, Geoff McAuliffe wrote: > > Hi Emily et al. > > The President, any President, can put $ for NIH is the budget request > he/she submits to Congress but ONLY Congress can appropriate money. > That is in the Constitution. > Several of my colleagues are out of work due to loss of grant support > 'cause NIH, while not broke, has many worthy projects to fund and > cannot fund them all. > > Geoff > > Emily Sours wrote: > >> Isn't the election over? >> Griping will do you no good, spambot! >> >> So, being in grant funded research, I hope our new president will put >> some money back into NIH. >> (I was hoping that no matter who won, so my political views will not >> spam >> you) >> Anyone else feel that way? >> >> Emily >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Wed Nov 5 11:41:54 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Nov 5 11:41:57 2008 Subject: [Histonet] Political SPAM In-Reply-To: Message-ID: All, Just an FYI: these political postings are SPAM. These are not real histonet users, they are generic postings that are fired everywhere like viagra solicitations. In other words, there is no real need to get upset with histonet users or administrators for random postings that everyone that has email or signs up for a listserv. will be getting by no choice or fault of their own. Glen Dawson Milwaukee, WI From sam.histology <@t> gmail.com Wed Nov 5 12:10:16 2008 From: sam.histology <@t> gmail.com (Sam Histology) Date: Wed Nov 5 12:10:20 2008 Subject: [Histonet] Gina Rodriguez is out of the office. In-Reply-To: References: Message-ID: > I will be out of the office starting 11/05/2008 and will not return until 11/10/2008. This kind of material really should not be posted on the HistoNet! Why are you posting something that's off-topic like this? This is a Histology list, not a 'I will be out of the office' list! They might shut down the list! From joachim.hehl <@t> lmc.biol.ethz.ch Wed Nov 5 12:09:55 2008 From: joachim.hehl <@t> lmc.biol.ethz.ch (Hehl Joachim) Date: Wed Nov 5 12:14:46 2008 Subject: [Histonet] president References: Message-ID: <19851AFF0FE7B44781D3B5A5A178C97F242730@EX1.d.ethz.ch> Please receive my apology. It was a provocation -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu im Auftrag von histonet-request@lists.utsouthwestern.edu Gesendet: Mi 05.11.2008 19:09 An: histonet@lists.utsouthwestern.edu Betreff: Histonet Digest, Vol 60, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. prostate core processing (karen adams) 2. Thank you! (HACKERLAB@aol.com) 3. President (Joachim Hehl) 4. Gina Rodriguez is out of the office. (Gina.Rodriguez@leica-microsystems.com) 5. What the heck is this doiing on the HistoNet??? (HACKERLAB@aol.com) 6. RE: What the heck is this doiing on the HistoNet??? (Mickie Johnson) 7. Fungal staining (Weaver, Colin) 8. Re: funding for NIH (Mark Tarango) 9. Re: Fungal staining (Larry Woody) 10. AW: [Histonet] Fungal staining (Gudrun Lang) 11. RE: funding for NIH (Bartlett, Jeanine (CDC/CCID/NCZVED)) 12. Political SPAM (Dawson, Glen) ---------------------------------------------------------------------- Message: 1 Date: Wed, 5 Nov 2008 10:10:51 -0500 From: "karen adams" Subject: [Histonet] prostate core processing To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Greetings, Can anyone offer a processing schedual for prostate bx cores for the Leica processor?? ------------------------------ Message: 2 Date: Wed, 5 Nov 2008 10:54:32 EST From: HACKERLAB@aol.com Subject: [Histonet] Thank you! To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Many thanks for intervening. Elfi Hacker Hacker Industries Winnsboro, SC Dear Histonetters: I am the list administrator and I would like to ask that people please refrain from further political comments on the list. I know this is a touchy topic but this is not the forum for these discussions. Please remember, my colleagues and I at the University of Texas Southwestern Medical Center run this list using University resources and it would be a real shame if they were to decide this was not a good use of their funds due to the off-topic comments etc. The Histonet list is an amazing collection of >3300 people from around the world helping each other and we really need to stick to topics pertinent to Histology. Thanks. Linda M Histonet administrator Linda Margraf, MD Professor of Pathology University of Texas Southwestern Medical School Dallas TX USA **************AOL Search: Your one stop for directions, recipes and all other Holiday needs. Search Now. (http://pr.atwola.com/promoclk/100000075x1212792382x1200798498/aol?redir=http://searchblog.aol.com/2008/11/04/happy-holidays-from -aol-search/?ncid=emlcntussear00000001) ------------------------------ Message: 3 Date: Wed, 05 Nov 2008 16:58:03 +0100 From: Joachim Hehl Subject: [Histonet] President To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Interesting, the histonet list seems to be occupied by ultraconservatives. ------------------------------ Message: 4 Date: Wed, 5 Nov 2008 10:00:57 -0600 From: Gina.Rodriguez@leica-microsystems.com Subject: [Histonet] Gina Rodriguez is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 11/05/2008 and will not return until 11/10/2008. I will respond to your message when I return. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 5 Date: Wed, 5 Nov 2008 10:52:25 EST From: HACKERLAB@aol.com Subject: [Histonet] What the heck is this doiing on the HistoNet??? To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" This kind of material really should not be posted on the HistoNet! Why don't you find some other place to vent! Elfi Hacker Hacker Industries Inc http://www.youtube.com/watch?v=wFYk7a3vEyo I don't know how many times I've heard the words "Obama" and "Messiah" used in the same sentence. To liberals, Barack Obama is a spotless god-like figure who will save America from the scourge of neo-conservatism. I'm totally sick of hearing and reading your hero-worshiping crap, folks. Let's face reality: *like so many other powerful politicians in this country, Barack Obama sucks*: 1. *The Race Card*: Whether it be in suggesting that anyone who doesn't vote for him because he is black is probably a republican, or in blaming Bush administration racismon a slow response to Hurricane Katrina, Obama is quite comfortable playing the race card. 2. *Anti-Indian*: After the Obama campaign released a paper disparaging other candidates for their ties to the Indian-American community, the chairman of the bipartisan US India Political Action Committee, Sanjay Puri, stated that the Obama Campaign was "engaging in the worst kind of anti-Indian American stereotyping." Of course, Obama denied any hand in the racist document put out by his campaign. 3. *Corrupt Buddies*: Tony Rezko, a long time friend and fund-raiser for Obama, was indicted last fall on federal charges that accuse him of demanding kickbacks from companies seeking state business. When asked about his friend, Obama said, "I've never done any favors for him." This turned out to be a lie, as evidence turned upprovin g that Obama had written letters to city and state officials praising Rezko's business practices. 4. *Wal-Mart Ties*: While bashing of Wal-Mart's labor practices in public, Obama has been profiting from their businessthrough the money his wife made as a member of the board of directors for a company that produces food for the mega-corporation. 5. *Religious Ties*: Is Obama a Muslim? Is he a Christian? Nobody is 100% sure, but it is true that Obama was raised in a Muslim family and at one time attended an Islamic school. He currently claims to be a convert to Christianity, but some are concerned about his Muslim upbringing . 6. *Anti-Second Amendment*: Obama is one of the most anti-Second Amendment legislators in the country. He supports a ban the sale or transfer of *all* forms of semi-automatic weapons. 7. *Gas-guzzler*: Obama might attack American automakers for not making enough environmental friendly automobiles, but when he goes home he drives a gas-guzzling V-8 hemi-powered Chrysler 300 . 8. *Obama Ringtones*: The most annoying campaign tool ever . 9. *Obama Girl*: I take back what I said about the ringtones. This girlis far more annoying. 10. *His Unelectable Name*: Barack Hussein Obama, 'nuff said. On Tue, Nov 4, 2008 at 6:47 PM, Linda Jones wrote: > > stupid!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! > > Linda Harper-Jones BS.,HT/ HTL(ASCP) > University of Mississippi Medical Center > Department of Pathology > Chief Histotechnologist Supervisor > Telephone (601) 984-1576 > Fax (601) 984-4968 > ljones@pathology.umsmed.edu > > > The information contained in this email is confidential. Individuals who > have received this information in error or are not authorized to receive it > must promptly return or dispose of the information and notify the sender. > Those individuals are hereby notified that they are strictly prohibited > from reviewing, forwarding, printing, copying, distributing or using this > information in any way. > > > >>> "Sam Histology" 11/04/08 8:48 AM >>> > Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama > Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann > Dunham > of Wichita, Kansas. (white atheist ). > > When Obama was two years old, his parents divorced and his father returned > to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta > with Obama when he was six years old. Within six months he had learned to > speak the Indonesian language. Obama spent "two years in a Muslim school, > then two more in a Catholic school" in Jakarta. Obama takes great care to > conceal the fact that he is a Muslim while admitting that he was once a > Muslim, mitigating that damning information by saying that, for two years, > he also attended a Catholic school. > > Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated > from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white > atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, > Sr. and Dunham divorced when Barack, Jr. was two. > > Obama's spinmeisters are now attempting to make it appear that Obama's > introduction to Islam came from his father and that influence was temporary > at best. > > In reality, the senior Obama returned to Kenya immediately following the > divorce and never again had any direct influence over his son's education. > > Dunham married another Muslim, Lolo Soetoro who educated his stepson as a > good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism > is the radical teaching that created the Muslim terrorists who are now > waging Jihad on the industrialized world. > > Since it is politically expedient to be a Christian when you are seeking > political office in the United States, Obama joined the United Church of > Christ to help purge any notion that he is still a Muslim. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Individuals who have received this information in error or are not > authorized to receive it must promptly return or dispose of the information > and notify the sender. Those individuals are hereby notified that they are > strictly prohibited from reviewing, forwarding, printing, copying, > distributing or using this information in any way. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************AOL Search: Your one stop for directions, recipes and all other Holiday needs. Search Now. (http://pr.atwola.com/promoclk/100000075x1212792382x1200798498/aol?redir=http://searchblog.aol.com/2008/11/04/happy-holidays-from -aol-search/?ncid=emlcntussear00000001) ------------------------------ Message: 6 Date: Wed, 5 Nov 2008 08:28:31 -0800 From: "Mickie Johnson" Subject: RE: [Histonet] What the heck is this doiing on the HistoNet??? To: , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Ditto! Best Regards, ? Mickie ? Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC ? & Mohs Lab Staffing & Mohs Suite Mohs Reporting Software 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX?? 509-624-3926 Web: www.mohshistogyconsulting.com?& www.mohslabstaffing.com & www.mohssuite.com Email: mickie25@netzero.net ? DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. Thank You. ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of HACKERLAB@aol.com Sent: Wednesday, November 05, 2008 7:52 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What the heck is this doiing on the HistoNet??? This kind of material really should not be posted on the HistoNet! Why don't you find some other place to vent! Elfi Hacker Hacker Industries Inc http://www.youtube.com/watch?v=wFYk7a3vEyo I don't know how many times I've heard the words "Obama" and "Messiah" used in the same sentence. To liberals, Barack Obama is a spotless god-like figure who will save America from the scourge of neo-conservatism. I'm totally sick of hearing and reading your hero-worshiping crap, folks. Let's face reality: *like so many other powerful politicians in this country, Barack Obama sucks*: 1. *The Race Card*: Whether it be in suggesting that anyone who doesn't vote for him because he is black is probably a republican, or in blaming Bush administration racismon a slow response to Hurricane Katrina, Obama is quite comfortable playing the race card. 2. *Anti-Indian*: After the Obama campaign released a paper disparaging other candidates for their ties to the Indian-American community, the chairman of the bipartisan US India Political Action Committee, Sanjay Puri, stated that the Obama Campaign was "engaging in the worst kind of anti-Indian American stereotyping." Of course, Obama denied any hand in the racist document put out by his campaign. 3. *Corrupt Buddies*: Tony Rezko, a long time friend and fund-raiser for Obama, was indicted last fall on federal charges that accuse him of demanding kickbacks from companies seeking state business. When asked about his friend, Obama said, "I've never done any favors for him." This turned out to be a lie, as evidence turned upprov in g that Obama had written letters to city and state officials praising Rezko's business practices. 4. *Wal-Mart Ties*: While bashing of Wal-Mart's labor practices in public, Obama has been profiting from their businessthrough the money his wife made as a member of the board of directors for a company that produces food for the mega-corporation. 5. *Religious Ties*: Is Obama a Muslim? Is he a Christian? Nobody is 100% sure, but it is true that Obama was raised in a Muslim family and at one time attended an Islamic school. He currently claims to be a convert to Christianity, but some are concerned about his Muslim upbringing . 6. *Anti-Second Amendment*: Obama is one of the most anti-Second Amendment legislators in the country. He supports a ban the sale or transfer of *all* forms of semi-automatic weapons. 7. *Gas-guzzler*: Obama might attack American automakers for not making enough environmental friendly automobiles, but when he goes home he drives a gas-guzzling V-8 hemi-powered Chrysler 300 . 8. *Obama Ringtones*: The most annoying campaign tool ever . 9. *Obama Girl*: I take back what I said about the ringtones. This girlis far more annoying. 10. *His Unelectable Name*: Barack Hussein Obama, 'nuff said. On Tue, Nov 4, 2008 at 6:47 PM, Linda Jones wrote: > > stupid!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! > > Linda Harper-Jones BS.,HT/ HTL(ASCP) > University of Mississippi Medical Center > Department of Pathology > Chief Histotechnologist Supervisor > Telephone (601) 984-1576 > Fax (601) 984-4968 > ljones@pathology.umsmed.edu > > > The information contained in this email is confidential. Individuals who > have received this information in error or are not authorized to receive it > must promptly return or dispose of the information and notify the sender. > Those individuals are hereby notified that they are strictly prohibited > from reviewing, forwarding, printing, copying, distributing or using this > information in any way. > > > >>> "Sam Histology" 11/04/08 8:48 AM >>> > Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama > Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann > Dunham > of Wichita, Kansas. (white atheist ). > > When Obama was two years old, his parents divorced and his father returned > to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta > with Obama when he was six years old. Within six months he had learned to > speak the Indonesian language. Obama spent "two years in a Muslim school, > then two more in a Catholic school" in Jakarta. Obama takes great care to > conceal the fact that he is a Muslim while admitting that he was once a > Muslim, mitigating that damning information by saying that, for two years, > he also attended a Catholic school. > > Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated > from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white > atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, > Sr. and Dunham divorced when Barack, Jr. was two. > > Obama's spinmeisters are now attempting to make it appear that Obama's > introduction to Islam came from his father and that influence was temporary > at best. > > In reality, the senior Obama returned to Kenya immediately following the > divorce and never again had any direct influence over his son's education. > > Dunham married another Muslim, Lolo Soetoro who educated his stepson as a > good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism > is the radical teaching that created the Muslim terrorists who are now > waging Jihad on the industrialized world. > > Since it is politically expedient to be a Christian when you are seeking > political office in the United States, Obama joined the United Church of > Christ to help purge any notion that he is still a Muslim. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Individuals who have received this information in error or are not > authorized to receive it must promptly return or dispose of the information > and notify the sender. Those individuals are hereby notified that they are > strictly prohibited from reviewing, forwarding, printing, copying, > distributing or using this information in any way. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************AOL Search: Your one stop for directions, recipes and all other Holiday needs. Search Now. (http://pr.atwola.com/promoclk/100000075x1212792382x1200798498/aol?redir=htt p://searchblog.aol.com/2008/11/04/happy-holidays-from -aol-search/?ncid=emlcntussear00000001) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.8.5/1763 - Release Date: 11/2/2008 7:08 PM ------------------------------ Message: 7 Date: Wed, 5 Nov 2008 17:02:37 -0000 From: "Weaver, Colin" Subject: [Histonet] Fungal staining To: Message-ID: <7A885E8FE1C71C488D974EC601FAA690041C23C6@vla-exchn1.cvlnt.vla.gov.uk> Content-Type: text/plain; charset="us-ascii" Hi - everyone - does anyone know of any fungi that can not be demonstrated by PAS stain? We appear to have one in a cow placenta which is obvious on Grocott and H&E but do not stain on PAS. The micro lab isolated Absidia spp but the hyphae in the Grocott are septate but I believe Absidia are aseptate. Any ideas would be most welcome. Colin Weaver VLA Thirsk England Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. ------------------------------ Message: 8 Date: Wed, 5 Nov 2008 09:16:43 -0800 From: "Mark Tarango" Subject: Re: [Histonet] funding for NIH To: "Geoff McAuliffe" Cc: histonet@lists.utsouthwestern.edu Message-ID: <5b6eb13e0811050916o74c6269ei1fb9fd0a91bfc79@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I definately hope that now that Obama is going to be President that the reseach will get moving again. He's got The Congress too. P.S. I hope this message doesn't shut the histonet down. Mark On 11/5/08, Geoff McAuliffe wrote: > > Hi Emily et al. > > The President, any President, can put $ for NIH is the budget request > he/she submits to Congress but ONLY Congress can appropriate money. That is > in the Constitution. > Several of my colleagues are out of work due to loss of grant support > 'cause NIH, while not broke, has many worthy projects to fund and cannot > fund them all. > > Geoff > > Emily Sours wrote: > >> Isn't the election over? >> Griping will do you no good, spambot! >> >> So, being in grant funded research, I hope our new president will put some >> money back into NIH. >> (I was hoping that no matter who won, so my political views will not spam >> you) >> Anyone else feel that way? >> >> Emily >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Wed, 5 Nov 2008 09:17:39 -0800 (PST) From: Larry Woody Subject: Re: [Histonet] Fungal staining To: "Weaver, Colin" , histonet@lists.utsouthwestern.edu Message-ID: <646095.66619.qm@web53607.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Maybe Aspergillus or Rhizopus. ? Larry A. Woody Seattle, Wa. ________________________________ From: "Weaver, Colin" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 5, 2008 9:02:37 AM Subject: [Histonet] Fungal staining Hi - everyone - does anyone know of any fungi that can not be demonstrated by PAS stain? We appear to have one in a cow placenta which is obvious on Grocott and H&E but do not stain on PAS. The micro lab isolated Absidia spp but the hyphae in the Grocott are septate but I believe Absidia are aseptate. Any ideas would be most welcome. Colin Weaver VLA Thirsk England ? Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 5 Nov 2008 18:18:21 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] Fungal staining To: Message-ID: <34E7498B0F784743A2AFE5C210716047@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Try a CAS stain. = PAS with Chromic acid Perhaps this fungus needs something stronger than Periodic acid. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Weaver, Colin Gesendet: Mittwoch, 05. November 2008 18:03 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Fungal staining Hi - everyone - does anyone know of any fungi that can not be demonstrated by PAS stain? We appear to have one in a cow placenta which is obvious on Grocott and H&E but do not stain on PAS. The micro lab isolated Absidia spp but the hyphae in the Grocott are septate but I believe Absidia are aseptate. Any ideas would be most welcome. Colin Weaver VLA Thirsk England Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 5 Nov 2008 12:38:13 -0500 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] funding for NIH To: "Mark Tarango" , "Geoff McAuliffe" Cc: histonet@lists.utsouthwestern.edu Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C46E@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii All I will say on the matter is that if more funding is approved I hope it is simply pulled from somewhere else and is not in addition to.....because that would mean more taxes! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Wednesday, November 05, 2008 12:17 PM To: Geoff McAuliffe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] funding for NIH I definately hope that now that Obama is going to be President that the reseach will get moving again. He's got The Congress too. P.S. I hope this message doesn't shut the histonet down. Mark On 11/5/08, Geoff McAuliffe wrote: > > Hi Emily et al. > > The President, any President, can put $ for NIH is the budget request > he/she submits to Congress but ONLY Congress can appropriate money. > That is in the Constitution. > Several of my colleagues are out of work due to loss of grant support > 'cause NIH, while not broke, has many worthy projects to fund and > cannot fund them all. > > Geoff > > Emily Sours wrote: > >> Isn't the election over? >> Griping will do you no good, spambot! >> >> So, being in grant funded research, I hope our new president will put >> some money back into NIH. >> (I was hoping that no matter who won, so my political views will not >> spam >> you) >> Anyone else feel that way? >> >> Emily >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 5 Nov 2008 11:41:54 -0600 From: "Dawson, Glen" Subject: [Histonet] Political SPAM To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" All, Just an FYI: these political postings are SPAM. These are not real histonet users, they are generic postings that are fired everywhere like viagra solicitations. In other words, there is no real need to get upset with histonet users or administrators for random postings that everyone that has email or signs up for a listserv. will be getting by no choice or fault of their own. Glen Dawson Milwaukee, WI ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 60, Issue 9 *************************************** From cbass <@t> wfubmc.edu Wed Nov 5 11:15:06 2008 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Wed Nov 5 12:18:51 2008 Subject: [Histonet] 200 proof alcohol? Message-ID: Hello, I?m trying to buy some alcohol for both histology and molecular biology protocols. But I?m having some difficult getting ?molecular biology grade? 100% ethanol. The hospital I work at sells 200 proof ethanol, but can?t say anything about the quality. Since it?s 200 proof, is that basically the same as molecular biology grade? How about histology grade? I seem to recall using hospital provided ethanol for molecular biology experiments in my postdoc, so I think it should be ok. Any advice is appreciated! Caroline From JWeems <@t> sjha.org Sat Nov 1 08:34:13 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Nov 5 12:24:11 2008 Subject: [Histonet] Re: bone marrow biopsies In-Reply-To: References: Message-ID: <10109467.1225546455268.JavaMail.root@ITSSAGW03> Read your secure message by opening the attachment, securedoc.html. You will be prompted to open (view) the file or save (download) it to your computer. For best results, save the file first, then open it in a Web browser. If you have concerns about the validity of this message, contact the sender directly. First time users - will need to register after opening the attachment. Help - https://securemail.che.org/websafe/help?topic=RegEnvelope About IronPort Encryption - https://securemail.che.org/websafe/about From kdwyer3322 <@t> aol.com Sun Nov 2 19:04:59 2008 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Wed Nov 5 12:24:13 2008 Subject: [Histonet] Texas Society for Histotechnology 2009 Call for Abstracts Message-ID: <8CB0B675449CC4A-D08-1063@webmail-dx11.sysops.aol.com> Histonetters, The Texas Society for Histotechnology will be holding it 2009 meeting in beautiful Austin Texas on May 28-31, 2009 (This is not Memorial weekend) at the Hyatt Regency Austin Lady Bird Lake, 208 Barton Springs, Austin, Texas.? The program committee is actively seeking workshop/symposum abstracts for this meeting.? If you or anyone you know would like to submit a workshop to the meeting please contact: Kathy Dwyer?- kdwyer3322@aol.com ? OR Veronica Davis - veronida@baylorhealth.edu Attached is the TSH 2009 Call for Abstracts. Thanks, TSH Convention Committe From kdwyer3322 <@t> aol.com Sun Nov 2 19:51:36 2008 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Wed Nov 5 12:24:14 2008 Subject: [Histonet] Fwd: Texas Society for Histotechnology 2009 Call for Abstracts In-Reply-To: <8CB0B675449CC4A-D08-1063@webmail-dx11.sysops.aol.com> References: <8CB0B675449CC4A-D08-1063@webmail-dx11.sysops.aol.com> Message-ID: <8CB0B6DD760CBB4-EA0-CC1@WEBMAIL-DF08.sysops.aol.com> -----Original Message----- From: kdwyer3322@aol.com To: histonet@lists.utsouthwestern.edu Sent: Sun, 2 Nov 2008 7:04 pm Subject: Texas Society for Histotechnology 2009 Call for Abstracts Histonetters, The Texas Society for Histotechnology will be holding it 2009 meeting in beautiful Austin Texas on May 28-31, 2009 (This is not Memorial weekend) at the Hyatt Regency Austin Lady Bird Lake, 208 Barton Springs, Austin, Texas.? The program committee is actively seeking workshop/symposum abstracts for this meeting.? If you or anyone you know would like to submit a workshop to the meeting please contact: Kathy Dwyer?- kdwyer3322@aol.com ? OR Veronica Davis - veronida@baylorhealth.edu Attached is the TSH 2009 Call for Abstracts. Thanks, TSH Convention Committe McCain or Obama? Stay up to date on the latest from the campaign trail with AOL News. From carrolpb <@t> umdnj.edu Wed Nov 5 11:49:59 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Wed Nov 5 12:24:27 2008 Subject: [Histonet] funding for NIH In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A70208C46E@LTA3VS011.ees.hhs.gov> References: <4911AC93.9080209@umdnj.edu> <5b6eb13e0811050916o74c6269ei1fb9fd0a91bfc79@mail.gmail.com> <1CE1847DFEA0A647B1CCDE4108EA60A70208C46E@LTA3VS011.ees.hhs.gov> Message-ID: <4911DCC7.4000004@umdnj.edu> > because that would mean more taxes! Making more than $250,000/year doing histology? Where do I sign up?! ;) From nrutledge <@t> CapeCodHealth.org Wed Nov 5 12:31:09 2008 From: nrutledge <@t> CapeCodHealth.org (Rutledge, Nancy) Date: Wed Nov 5 12:31:18 2008 Subject: [Histonet] Cytology QA Plan Message-ID: <47AD3B259E920D449F580E6AE82C2B8F210272@FHEXSVR2.FHDOMAIN1.capecodhealth.org> We just started doing NONGYN cytology. We have no cytologist. Does anyone have a suggestion and/or a starting place for how to develop a QA Plan. Thanks in advance. Nancy ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From jqb7 <@t> cdc.gov Wed Nov 5 12:36:54 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Nov 5 12:48:01 2008 Subject: [Histonet] funding for NIH In-Reply-To: <4911DCC7.4000004@umdnj.edu> References: <4911AC93.9080209@umdnj.edu> <5b6eb13e0811050916o74c6269ei1fb9fd0a91bfc79@mail.gmail.com> <1CE1847DFEA0A647B1CCDE4108EA60A70208C46E@LTA3VS011.ees.hhs.gov> <4911DCC7.4000004@umdnj.edu> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C472@LTA3VS011.ees.hhs.gov> unfortunately, all government funding comes from us peons.......that's the way it has always been and will always be. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peter Carroll Sent: Wednesday, November 05, 2008 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] funding for NIH > because that would mean more taxes! Making more than $250,000/year doing histology? Where do I sign up?! ;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dennijc <@t> vetmed.auburn.edu Wed Nov 5 13:04:15 2008 From: dennijc <@t> vetmed.auburn.edu (John C. Dennis) Date: Wed Nov 5 13:04:25 2008 Subject: [Histonet] 200 proof alcohol? In-Reply-To: References: Message-ID: Caroline I would not recommend using the same alcohol for histo that you use for molecular work. Assuming by "molecular" you mean isolating DNA/RNA, cloning, etc. You'll want a higher grade, more expensive alcohol for nucleic acid work then the histological stuff that comes in a 55 gal drum. John Carroll Dennis Anatomy, Physiology, and Pharmacology 109 Greene Hall Auburn University, AL 36849 On Wed, 5 Nov 2008, Caroline Bass wrote: > Hello, > > I?m trying to buy some alcohol for both histology and molecular biology > protocols. But I?m having some difficult getting ?molecular biology grade? > 100% ethanol. The hospital I work at sells 200 proof ethanol, but can?t say > anything about the quality. Since it?s 200 proof, is that basically the same > as molecular biology grade? How about histology grade? I seem to recall > using hospital provided ethanol for molecular biology experiments in my > postdoc, so I think it should be ok. > > Any advice is appreciated! > > Caroline > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Dorothy.L.Webb <@t> HealthPartners.Com Wed Nov 5 14:21:46 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Nov 5 14:21:52 2008 Subject: [Histonet] Fatty tissue Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635AB4@hpes1.HealthPartners.int> I would like to know how other labs are processing "fatty" tissue, especially breast for optimal processing? We have been doing a few "reprocessing" of these specimens and my pathologist feels this should not be happening! Yes, the PA's are, at times, cutting the sections larger than 3 mm thick, but, at times the tumor is ingrained in the fatty area and they need to obtain as much of the perimeter as they can. Does anyone agitate or heat fixate their specimens prior to processing? Would appreciate any feedback on this age old dilemma. Also, I only heard back from one person on the recycling issue. Any input would be appreciated as to savings incurred and a still vs. recycler. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From MadaryJ <@t> MedImmune.com Wed Nov 5 14:39:46 2008 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Wed Nov 5 14:39:58 2008 Subject: [Histonet] Male and female fungal organisms? Chromic and periodic acid Message-ID: Gridley Fungus is a stain that uses chromic as the oxidizer and schiffs reagent but finishes off with aldehyde fuchsin(and maybe Metanil Yellow?), and provides a light stain, but great for morphology. I do not know how specific it is but when I was at AFIP in the Infectious Disease Lab, we used it a lot in fungal panels. What did the female mushroom say to the male mushroom? Hey you are a real fun guy(get it fun gi?). I made a funny. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory Mgr One Medimmune Way, Lab 2438-Area 4 Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Wed Nov 5 14:44:41 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Nov 5 14:44:46 2008 Subject: [Histonet] Fatty tissue In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2705635AB4@hpes1.HealthPartners.int> Message-ID: <919637.33072.qm@web65714.mail.ac4.yahoo.com> Dorothy: "Reprocessing" fatty tissue (usually a hopeless proposition) is essentially caused by poor infiltration more than by poor fixation, and poor infiltration is usually caused by less than optimal time in the clearing agent or antemedium (usually xylene). The thing is that fatty tissue contains less water than other tissues and will dehydrate excessively in the routine protocol, but requires more clearing time than in the "normal" protocols. The solution is to shorten the dehydration period and increase the clearing times or, as I solved the problem, by eliminating xylene altogether when I started using a clearing mixture composed of isopropyl alcohol and mineral oil. Ren? J. --- On Wed, 11/5/08, Webb, Dorothy L wrote: From: Webb, Dorothy L Subject: [Histonet] Fatty tissue To: histonet@lists.utsouthwestern.edu Date: Wednesday, November 5, 2008, 3:21 PM I would like to know how other labs are processing "fatty" tissue, especially breast for optimal processing? We have been doing a few "reprocessing" of these specimens and my pathologist feels this should not be happening! Yes, the PA's are, at times, cutting the sections larger than 3 mm thick, but, at times the tumor is ingrained in the fatty area and they need to obtain as much of the perimeter as they can. Does anyone agitate or heat fixate their specimens prior to processing? Would appreciate any feedback on this age old dilemma. Also, I only heard back from one person on the recycling issue. Any input would be appreciated as to savings incurred and a still vs. recycler. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sam.histology <@t> gmail.com Wed Nov 5 15:08:51 2008 From: sam.histology <@t> gmail.com (Sam Histology) Date: Wed Nov 5 15:08:54 2008 Subject: [Histonet] Re: bone marrow biopsies In-Reply-To: <10109467.1225546455268.JavaMail.root@ITSSAGW03> References: <10109467.1225546455268.JavaMail.root@ITSSAGW03> Message-ID: At home, there will be an increase in taxes?income, estate, payroll?to fund more government health care, education, and general entitlement programs. The old Reaganesque notion that government subsidies can make one more dependent, angrier, and envious is forgotten, along with the notion that lower taxes stimulate economic growth and encourage risk-taking, innovation, and independence. I worry especially about the lifting of income caps (how far?) on social security taxes inasmuch as they were part of the original covenant justifying the caps on benefits paid out. NAFTA and other free trade agreements would be repealed; illegal immigration would either not be an issue, or more a problem of finding the right way, with borders still open, to grant amnesties. Appointments would hinge on a belief in bigger government and the theme that the individual is currently suffering due to reactionaries in government and corporations, barely housed, fed, or educated, and deserves more federal dollars appropriated from others who either don't need all their income or didn't deserve the compensation they were given. Abroad, there is a general argument that things are going terribly. Forget that the Taliban and Saddam are gone. Forget that we have not suffered another 9/11 attack. Forget that there is far more democratic promise in Afghanistan, Iraq, Pakistan, and Lebanon than was true in 2001. Forget that the Merkel and Sarkozy governments, along with Eastern European leaders, are more pro-American than their predecessors in 2001. Instead, we are disliked by everyone, and for good reasons. The fact that Iranian mullahs, the House of Saud cousins, Hugo Chavez's communists, European mullahs, and the Arab street don't approve of America says more about us than it does them. The solution is to follow more the dictates of European Union and United Nations, where sophisticated internationalists can guide us through the maze of global power, instructing mostly ignorant Americans how and why we tend to cause so many of the world's problems. Misunderstanding and our own obtuseness explain global tension, not the agendas of enemies who know exactly what they want and how to get it. Our military is not so much an offensive force, designed to defeat and kill our enemies, that needs support and constant honing; better to see it as a large social organization that we must look at in terms only of proper rotations, health care, and benefits. We are to support the troops not in the sense of doing everything we can to ensure they win, and gain the proper recognition for their courage and sacrifice, but rather in consideration of their victimhood, offering proper sympathy and remediation for the defeat in Iraq, the unwise use of their skills, and the needless loss of their lives. From pieronelva01 <@t> bigpond.com Wed Nov 5 15:13:38 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Wed Nov 5 15:13:44 2008 Subject: [Histonet] re: Biocare Decloaker HIER References: <394A9DE849AA4B6B9661DF4CF0C6274E@auxs.umn.edu> <49118C7E.7000207@bms.com> Message-ID: <80C22A182E98445485C37EEE5D316F09@pentium4> Ooops. One too many ones! Piero ----- Original Message ----- From: "Linda M Watson" To: "Piero Nelva" Cc: "histonet" Sent: Wednesday, November 05, 2008 11:07 PM Subject: Re: [Histonet] re: Biocare Decloaker HIER > Is the 118 psi a typo? I don't believe the instrument can go that > high-max psi is 30. > > Piero Nelva wrote: > >> I used it for 4 minutes at about 118 psi and most antibodies worked >> really well. I was having huge problems with the Er and Her2 and this >> solved them (almost) overnight. >> >> >> ----- Original Message ----- From: "Jan Shivers" >> To: "histonet" >> Sent: Wednesday, November 05, 2008 3:12 AM >> Subject: [Histonet] re: Biocare Decloaker HIER >> >> >> For those of you who use Biocare's Decloaker for HIER on regular cases >> (not prion protein retrieval), could you tell me how long you do HIER >> with these machines? I normally do my prion cases in a Decloaker for >> 20', with a 25' cooldown, but I suspect that regular tissues don't >> need that length of time at high pressure/temp. I have an extra >> Decloaker and am considering switching to this device for my normal >> case work HIER, if it's time efficient. >> >> Please reply privately. Thanks. >> >> Jan Shivers >> Senior Scientist >> Histology/IHC/EM Section Head >> Pathology Teaching Program >> University of Minnesota >> Veterinary Diagnostic Laboratory >> 1333 Gortner Ave. >> St. Paul, MN 55108 >> 612-624-7297 >> shive003@umn.edu >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> -------------------------------------------------------------------------------- >> >> >> >> >> No virus found in this incoming message. >> Checked by AVG - http://www.avg.com >> Version: 8.0.175 / Virus Database: 270.8.6/1765 - Release Date: >> 11/3/2008 4:59 PM >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.8.6/1768 - Release Date: 11/4/2008 9:38 PM From vazquezr <@t> ohsu.edu Wed Nov 5 15:24:58 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Wed Nov 5 15:25:09 2008 Subject: [Histonet] Re: bone marrow biopsies In-Reply-To: References: <10109467.1225546455268.JavaMail.root@ITSSAGW03> Message-ID: <2A582E8156B45F468A62D1F1D20AF083426AAE@EX-BE08.ohsu.edu> Can you block this person? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sam Histology Sent: Wednesday, November 05, 2008 1:09 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: bone marrow biopsies At home, there will be an increase in taxes?income, estate, payroll?to fund more government health care, education, and general entitlement programs. The old Reaganesque notion that government subsidies can make one more dependent, angrier, and envious is forgotten, along with the notion that lower taxes stimulate economic growth and encourage risk-taking, innovation, and independence. I worry especially about the lifting of income caps (how far?) on social security taxes inasmuch as they were part of the original covenant justifying the caps on benefits paid out. NAFTA and other free trade agreements would be repealed; illegal immigration would either not be an issue, or more a problem of finding the right way, with borders still open, to grant amnesties. Appointments would hinge on a belief in bigger government and the theme that the individual is currently suffering due to reactionaries in government and corporations, barely housed, fed, or educated, and deserves more federal dollars appropriated from others who either don't need all their income or didn't deserve the compensation they were given. Abroad, there is a general argument that things are going terribly. Forget that the Taliban and Saddam are gone. Forget that we have not suffered another 9/11 attack. Forget that there is far more democratic promise in Afghanistan, Iraq, Pakistan, and Lebanon than was true in 2001. Forget that the Merkel and Sarkozy governments, along with Eastern European leaders, are more pro-American than their predecessors in 2001. Instead, we are disliked by everyone, and for good reasons. The fact that Iranian mullahs, the House of Saud cousins, Hugo Chavez's communists, European mullahs, and the Arab street don't approve of America says more about us than it does them. The solution is to follow more the dictates of European Union and United Nations, where sophisticated internationalists can guide us through the maze of global power, instructing mostly ignorant Americans how and why we tend to cause so many of the world's problems. Misunderstanding and our own obtuseness explain global tension, not the agendas of enemies who know exactly what they want and how to get it. Our military is not so much an offensive force, designed to defeat and kill our enemies, that needs support and constant honing; better to see it as a large social organization that we must look at in terms only of proper rotations, health care, and benefits. We are to support the troops not in the sense of doing everything we can to ensure they win, and gain the proper recognition for their courage and sacrifice, but rather in consideration of their victimhood, offering proper sympathy and remediation for the defeat in Iraq, the unwise use of their skills, and the needless loss of their lives. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Wed Nov 5 15:53:41 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Wed Nov 5 15:53:46 2008 Subject: [Histonet] Re: bone marrow biopsies References: <10109467.1225546455268.JavaMail.root@ITSSAGW03> <2A582E8156B45F468A62D1F1D20AF083426AAE@EX-BE08.ohsu.edu> Message-ID: <703565.93001.qm@web53604.mail.re2.yahoo.com> Even better we will find where he is. ? Larry A. Woody Seattle, Wa. ________________________________ From: Robyn Vazquez To: Sam Histology ; Histonet@lists.utsouthwestern.edu Sent: Wednesday, November 5, 2008 1:24:58 PM Subject: RE: [Histonet] Re: bone marrow biopsies Can you block this person? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sam Histology Sent: Wednesday, November 05, 2008 1:09 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: bone marrow biopsies At home, there will be an increase in taxes?income, estate, payroll?to fund more government health care, education, and general entitlement programs. The old Reaganesque notion that government subsidies can make one more dependent, angrier, and envious is forgotten, along with the notion that lower taxes stimulate economic growth and encourage risk-taking, innovation, and independence. I worry especially about the lifting of income caps (how far?) on social security taxes inasmuch as they were part of the original covenant justifying the caps on benefits paid out. NAFTA and other free trade agreements would be repealed; illegal immigration would either not be an issue, or more a problem of finding the right way, with borders still open, to grant amnesties. Appointments would hinge on a belief in bigger government and the theme that the individual is currently suffering due to reactionaries in government and corporations, barely housed, fed, or educated, and deserves more federal dollars appropriated from others who either don't need all their income or didn't deserve the compensation they were given. Abroad, there is a general argument that things are going terribly. Forget that the Taliban and Saddam are gone. Forget that we have not suffered another 9/11 attack. Forget that there is far more democratic promise in Afghanistan, Iraq, Pakistan, and Lebanon than was true in 2001. Forget that the Merkel and Sarkozy governments, along with Eastern European leaders, are more pro-American than their predecessors in 2001. Instead, we are disliked by everyone, and for good reasons. The fact that Iranian mullahs, the House of Saud cousins, Hugo Chavez's communists, European mullahs, and the Arab street don't approve of America says more about us than it does them. The solution is to follow more the dictates of European Union and United Nations, where sophisticated internationalists can guide us through the maze of global power, instructing mostly ignorant Americans how and why we tend to cause so many of the world's problems. Misunderstanding and our own obtuseness explain global tension, not the agendas of enemies who know exactly what they want and how to get it. Our military is not so much an offensive force, designed to defeat and kill our enemies, that needs support and constant honing; better to see it as a large social organization that we must look at in terms only of proper rotations, health care, and benefits. We are to support the troops not in the sense of doing everything we can to ensure they win, and gain the proper recognition for their courage and sacrifice, but rather in consideration of their victimhood, offering proper sympathy and remediation for the defeat in Iraq, the unwise use of their skills, and the needless loss of their lives. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Nov 5 16:27:01 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Nov 5 16:27:06 2008 Subject: [Histonet] stain Message-ID: <67D3A267B931482BB6C4374ED4F091BD@Patsyoffice> Anyone familiar with this stain? Altmann acid-fushsin picric. This stain is a histochemical stain for mitochondria in intestine. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From AnthonyH <@t> chw.edu.au Wed Nov 5 16:42:41 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Nov 5 16:43:40 2008 Subject: [Histonet] Fatty tissue In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2705635AB4@hpes1.HealthPartners.int> Message-ID: I would suggest microwaving the tissue blocks in formalin for at least 1 hour (longer is better) up to a temperature of 45oC (you might even consider a higher temp maybe up to 55oC??) We use this with our placenta blocks and the results were dramatically improved (35% poorly processed to less than 1% after microwaving). We have a Mega TT Microwave oven Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, 6 November 2008 7:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fatty tissue I would like to know how other labs are processing "fatty" tissue, especially breast for optimal processing? We have been doing a few "reprocessing" of these specimens and my pathologist feels this should not be happening! Yes, the PA's are, at times, cutting the sections larger than 3 mm thick, but, at times the tumor is ingrained in the fatty area and they need to obtain as much of the perimeter as they can. Does anyone agitate or heat fixate their specimens prior to processing? Would appreciate any feedback on this age old dilemma. Also, I only heard back from one person on the recycling issue. Any input would be appreciated as to savings incurred and a still vs. recycler. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Wed Nov 5 16:48:34 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Nov 5 16:48:43 2008 Subject: [Histonet] Re: bone marrow biopsies In-Reply-To: <2A582E8156B45F468A62D1F1D20AF083426AAE@EX-BE08.ohsu.edu> Message-ID: Only if he is well fixed first!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Thursday, 6 November 2008 8:25 AM To: Sam Histology; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: bone marrow biopsies Can you block this person? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sam Histology Sent: Wednesday, November 05, 2008 1:09 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: bone marrow biopsies At home, there will be an increase in taxes-income, estate, payroll-to fund more government health care, education, and general entitlement programs. The old Reaganesque notion that government subsidies can make one more dependent, angrier, and envious is forgotten, along with the notion that lower taxes stimulate economic growth and encourage risk-taking, innovation, and independence. I worry especially about the lifting of income caps (how far?) on social security taxes inasmuch as they were part of the original covenant justifying the caps on benefits paid out. NAFTA and other free trade agreements would be repealed; illegal immigration would either not be an issue, or more a problem of finding the right way, with borders still open, to grant amnesties. Appointments would hinge on a belief in bigger government and the theme that the individual is currently suffering due to reactionaries in government and corporations, barely housed, fed, or educated, and deserves more federal dollars appropriated from others who either don't need all their income or didn't deserve the compensation they were given. Abroad, there is a general argument that things are going terribly. Forget that the Taliban and Saddam are gone. Forget that we have not suffered another 9/11 attack. Forget that there is far more democratic promise in Afghanistan, Iraq, Pakistan, and Lebanon than was true in 2001. Forget that the Merkel and Sarkozy governments, along with Eastern European leaders, are more pro-American than their predecessors in 2001. Instead, we are disliked by everyone, and for good reasons. The fact that Iranian mullahs, the House of Saud cousins, Hugo Chavez's communists, European mullahs, and the Arab street don't approve of America says more about us than it does them. The solution is to follow more the dictates of European Union and United Nations, where sophisticated internationalists can guide us through the maze of global power, instructing mostly ignorant Americans how and why we tend to cause so many of the world's problems. Misunderstanding and our own obtuseness explain global tension, not the agendas of enemies who know exactly what they want and how to get it. Our military is not so much an offensive force, designed to defeat and kill our enemies, that needs support and constant honing; better to see it as a large social organization that we must look at in terms only of proper rotations, health care, and benefits. We are to support the troops not in the sense of doing everything we can to ensure they win, and gain the proper recognition for their courage and sacrifice, but rather in consideration of their victimhood, offering proper sympathy and remediation for the defeat in Iraq, the unwise use of their skills, and the needless loss of their lives. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From jnocito <@t> satx.rr.com Wed Nov 5 18:23:09 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Nov 5 18:23:12 2008 Subject: [Histonet] 200 proof alcohol? References: Message-ID: <51185D01EA0E4817A796D117CDE7D1BA@yourxhtr8hvc4p> 200 proof is 200 proof, can't get any purer than that. Of course, I would take the taste test first. Green olives stuffed with Feta cheese goes good. JTT ----- Original Message ----- From: "Caroline Bass" To: Sent: Wednesday, November 05, 2008 11:15 AM Subject: [Histonet] 200 proof alcohol? Hello, I?m trying to buy some alcohol for both histology and molecular biology protocols. But I?m having some difficult getting ?molecular biology grade? 100% ethanol. The hospital I work at sells 200 proof ethanol, but can?t say anything about the quality. Since it?s 200 proof, is that basically the same as molecular biology grade? How about histology grade? I seem to recall using hospital provided ethanol for molecular biology experiments in my postdoc, so I think it should be ok. Any advice is appreciated! Caroline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Wed Nov 5 18:35:18 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Wed Nov 5 18:35:32 2008 Subject: [Histonet] stain References: <67D3A267B931482BB6C4374ED4F091BD@Patsyoffice> Message-ID: Altmann's acid fuchsin picric acid is a histological stain for mitochondria. It requres the tissue to be fixed in a non-acid dichromate fixative, then post-chromed for some time. The tissue is then processed by paraffin and sectioned. Staining is in aniline water saturated with acid fuchsin, which is applied much like carbol fuchsin in a ZN, i.e. heated. Diffeerentiation is with picric acid. The method requires considerable skill from the technologist, but produces red mitochondria on a yellow background. I suggest that if you just want to demonstrate mitochondria, do a succinate dehydrogenase instead, it is much easier and much faster. If you want, I can look up the details and send them on, but I recall they used to be in Culling's book. Bryan Llewellyn ----- Original Message ----- From: "Patsy Ruegg" To: Sent: Wednesday, November 05, 2008 2:27 PM Subject: [Histonet] stain > Anyone familiar with this stain? > > > > Altmann acid-fushsin picric. This stain is a histochemical stain for > mitochondria in intestine. > > > > Best regards, > > > > Patsy > > > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of the > Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that > is > privileged & confidential within the meaning of applicable law. > Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. > If > you are NOT the intended recipient please contact the sender and dispose > of > this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nicholasprosenbaum <@t> yahoo.com Wed Nov 5 18:47:08 2008 From: nicholasprosenbaum <@t> yahoo.com (Nicholas Rosenbaum) Date: Wed Nov 5 18:47:12 2008 Subject: [Histonet] ASCP HT maintenance fees References: <582736990811041639g4da10444sc7dd18c32dd4b4b4@mail.gmail.com> Message-ID: <312746.64360.qm@web35205.mail.mud.yahoo.com> Amen, Amos! Amen! Nicholas Rosenbaum HT, (ASCP) ________________________________ From: Amos Brooks To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, November 4, 2008 7:39:51 PM Subject: [Histonet] ASCP HT maintenance fees Hi, I don't mean to be the fly in the ointment here, but in the words of Janet Jackson "What have you done for me lately?". It's a long standing joke among sticker collectors that aside from the certification the sticker is all you really get from the society. Where was the ASCP when NYS decided to pass that whacky law causing histotechs basically to have to be medtechs? The legislation seemed to actually take even them by surprise. There was little warning that this was going to happen. The main school for histology (SUNY Cobleskill) actually closed for a while after this. The proper thing for an advocate for histotechnology woule be to inform the profession of upcoming changes. Help those affected, and certainly make sure the educational requirements are fully lined up prior to the final passage of the laws. That is if they were paying attention. Where was ASCP? Worrying about medtechs perhaps. To my eye ASCP has much less interest in histotechnology (after certification) than histotechnology has in the ASCP. Just an observation, Amos Message: 12 Date: Tue, 4 Nov 2008 08:23:02 -0700 From: "Patsy Ruegg" Subject: RE: [Histonet] ASCP HT maintenance fees To: "'R C'" , "'Podawiltz, Thomas'" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" One of the benefits of ASCP membership is to support their efforts to fight for the medical technology profession in local and national legislation. With shortages, licensure, billing and all sorts of political issues that affect our lives we need someone to be working for us. CAP and CLIA does not do much for HT's since they still do not even require that a certified HT be doing the job. I get reports almost daily on what ASCP is doing for us and I support it by paying my membership dues. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Wed Nov 5 19:32:26 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Wed Nov 5 19:35:49 2008 Subject: [Histonet] Re: bone marrow biopsies References: <10109467.1225546455268.JavaMail.root@ITSSAGW03> Message-ID: <08A0A863637F1349BBFD83A96B27A50A1201A8@uwhis-xchng3.uwhis.hosp.wisc.edu> Hey gang - I have taken a bit of a proactive approach to this spamming and contacted google e-mail (where 'Sam' sends little notes from) about our little problem. I don't know if it will do any good, but I did remind them that this activity IS against their use policy. Hopefully we won't be hearing from 'Sam' any more after this. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sam Histology Sent: Wed 11/5/2008 3:08 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: bone marrow biopsies At home, there will be an increase in taxes-income, estate, payroll-to fund more government health care, education, and general entitlement programs. The old Reaganesque notion that government subsidies can make one more dependent, angrier, and envious is forgotten, along with the notion that lower taxes stimulate economic growth and encourage risk-taking, innovation, and independence. I worry especially about the lifting of income caps (how far?) on social security taxes inasmuch as they were part of the original covenant justifying the caps on benefits paid out. NAFTA and other free trade agreements would be repealed; illegal immigration would either not be an issue, or more a problem of finding the right way, with borders still open, to grant amnesties. Appointments would hinge on a belief in bigger government and the theme that the individual is currently suffering due to reactionaries in government and corporations, barely housed, fed, or educated, and deserves more federal dollars appropriated from others who either don't need all their income or didn't deserve the compensation they were given. Abroad, there is a general argument that things are going terribly. Forget that the Taliban and Saddam are gone. Forget that we have not suffered another 9/11 attack. Forget that there is far more democratic promise in Afghanistan, Iraq, Pakistan, and Lebanon than was true in 2001. Forget that the Merkel and Sarkozy governments, along with Eastern European leaders, are more pro-American than their predecessors in 2001. Instead, we are disliked by everyone, and for good reasons. The fact that Iranian mullahs, the House of Saud cousins, Hugo Chavez's communists, European mullahs, and the Arab street don't approve of America says more about us than it does them. The solution is to follow more the dictates of European Union and United Nations, where sophisticated internationalists can guide us through the maze of global power, instructing mostly ignorant Americans how and why we tend to cause so many of the world's problems. Misunderstanding and our own obtuseness explain global tension, not the agendas of enemies who know exactly what they want and how to get it. Our military is not so much an offensive force, designed to defeat and kill our enemies, that needs support and constant honing; better to see it as a large social organization that we must look at in terms only of proper rotations, health care, and benefits. We are to support the troops not in the sense of doing everything we can to ensure they win, and gain the proper recognition for their courage and sacrifice, but rather in consideration of their victimhood, offering proper sympathy and remediation for the defeat in Iraq, the unwise use of their skills, and the needless loss of their lives. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Wed Nov 5 21:29:14 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Nov 5 21:29:19 2008 Subject: [Histonet] Re: Fungal stains Message-ID: Colin Weaver asks about staining of various fungal species by the standard fungal stains. The standard fungal stains have two basic steps: 1. Periodic acid or chromic acid splits bonds between vicinal diol groups (two carbons with OH"s on adjacent carbon atoms) and oxidizes the free ends to aldehyde groups (-CHO). 2. The aldehyde groups oxidize Schiff's reagent or methenamine silver to produce a visible deposit. Periodic acid is not a sufficiently strong oxidant to demonstrate some fungal cell walls. In human pathology, Histoplasma is most likely to stain weakly or not at all. You need chromic acid, most usually followed by methenamine silver. Unfortunately, many so-called GMS kits silently substitute periodic acid for the more hazardous chromic acid. Freida Carson reviewed this top in J Histotechnol several years ago - I can find the reference if anyone needs it (I think I've posted it on Histonet before, so it's probably in the archives.) So your first step is to see if you're actually using chromic acid. PLEASE get the politics off this list - the repetitious garbage is making the list hard to read, particularly in digest form. Linda Margraf, if it doesn't stop, I suggest moderating the list for a few days, though not permanently. Bob Richmond Samurai Pathologist Knoxville TN From akemiat3377 <@t> yahoo.com Wed Nov 5 21:56:16 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Nov 5 21:56:20 2008 Subject: [Histonet] Re: Fungal stains & political views In-Reply-To: Message-ID: <811755.43112.qm@web31302.mail.mud.yahoo.com> Hi All, I totally agree with Robert on his information regarding periodic acid and chromic acid. I have done extensive research on this subject when I developed my special stain kits for Biocare Medical. I did incorporate 10% chromic acid for the GMS stain, and 1% periodic acid for the PAS stain. As far as this forum for political views, in frustration, I deleted everything with these headings. There could of been some very interesting feed-back, but you have used this source totally inappropriately! I believe in this countries beliefs for freedom of speech, but use it wisely!! The histonet is NOT THE PLACE! Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 ext 1053 E-Mail: akemiat3377@yahoo.com --- On Wed, 11/5/08, Robert Richmond wrote: > From: Robert Richmond > Subject: [Histonet] Re: Fungal stains > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, November 5, 2008, 7:29 PM > Colin Weaver asks about staining of various fungal species > by the > standard fungal stains. > > The standard fungal stains have two basic steps: > > 1. Periodic acid or chromic acid splits bonds between > vicinal diol > groups (two carbons with OH"s on adjacent carbon > atoms) and oxidizes > the free ends to aldehyde groups (-CHO). > > 2. The aldehyde groups oxidize Schiff's reagent or > methenamine silver > to produce a visible deposit. > > Periodic acid is not a sufficiently strong oxidant to > demonstrate some > fungal cell walls. In human pathology, Histoplasma is most > likely to > stain weakly or not at all. You need chromic acid, most > usually > followed by methenamine silver. > > Unfortunately, many so-called GMS kits silently substitute > periodic > acid for the more hazardous chromic acid. Freida Carson > reviewed this > top in J Histotechnol several years ago - I can find the > reference if > anyone needs it (I think I've posted it on Histonet > before, so it's > probably in the archives.) So your first step is to see if > you're > actually using chromic acid. > > PLEASE get the politics off this list - the repetitious > garbage is > making the list hard to read, particularly in digest form. > Linda > Margraf, if it doesn't stop, I suggest moderating the > list for a few > days, though not permanently. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mariakatleba <@t> aol.com Thu Nov 6 00:05:52 2008 From: mariakatleba <@t> aol.com (Maria Katleba) Date: Thu Nov 6 00:05:56 2008 Subject: [Histonet] Maria added you as a friend on Reunion.com! Message-ID: <1207176510.646706@aol.com> Hi, I looked for you on Reunion.com, but you weren't there. Please connect with me so we can keep in touch. -Maria Do You Know Maria? YES - Connect with Maria, and see who's searching for you http://smtp26.mail.reunion.com:80/track?type=click&mailingid=13500&messageid=3500&databaseid=1223409114&serial=1207176510&emailid=histonet@lists.utsouthwestern.edu&userid=646706&extra=&&&2002&&&http://www.reunion.com/showInviteRegistration.do?uid=297786637 NO - I don't know Maria http://smtp26.mail.reunion.com:80/track?type=click&mailingid=13500&messageid=3500&databaseid=1223409114&serial=1207176510&emailid=histonet@lists.utsouthwestern.edu&userid=646706&extra=&&&2000&&&http://www.reunion.com/showInviteRegistration.do?unsub=true&invitee=histonet@lists.utsouthwestern.edu&uid=297786637 ---------------------------- Reunion.com - Find Everyone from Your Past. You have received this e-mail because a Reunion.com Member sent an invitation to this e-mail address. For assistance, please refer to our FAQ or Contact Us. http://smtp26.mail.reunion.com:80/track?type=click&mailingid=13500&messageid=3500&databaseid=1223409114&serial=1207176510&emailid=histonet@lists.utsouthwestern.edu&userid=646706&extra=&&&2001&&&http://help.reunion.com/selfhelp?lid=2 Our Address: 2118 Wilshire Blvd., Box 1008, Santa Monica, CA 90403-5784 From mariakatleba <@t> aol.com Thu Nov 6 00:05:52 2008 From: mariakatleba <@t> aol.com (Maria Katleba) Date: Thu Nov 6 00:05:58 2008 Subject: [Histonet] Maria added you as a friend on Reunion.com! Message-ID: <1207176510.646707@aol.com> Hi, I looked for you on Reunion.com, but you weren't there. Please connect with me so we can keep in touch. -Maria Do You Know Maria? YES - Connect with Maria, and see who's searching for you http://smtp26.mail.reunion.com:80/track?type=click&mailingid=13500&messageid=3500&databaseid=1223409114&serial=1207176510&emailid=histonet@lists.utsouthwestern.edu&userid=646707&extra=&&&2002&&&http://www.reunion.com/showInviteRegistration.do?uid=297786637 NO - I don't know Maria http://smtp26.mail.reunion.com:80/track?type=click&mailingid=13500&messageid=3500&databaseid=1223409114&serial=1207176510&emailid=histonet@lists.utsouthwestern.edu&userid=646707&extra=&&&2000&&&http://www.reunion.com/showInviteRegistration.do?unsub=true&invitee=histonet@lists.utsouthwestern.edu&uid=297786637 ---------------------------- Reunion.com - Find Everyone from Your Past. You have received this e-mail because a Reunion.com Member sent an invitation to this e-mail address. For assistance, please refer to our FAQ or Contact Us. http://smtp26.mail.reunion.com:80/track?type=click&mailingid=13500&messageid=3500&databaseid=1223409114&serial=1207176510&emailid=histonet@lists.utsouthwestern.edu&userid=646707&extra=&&&2001&&&http://help.reunion.com/selfhelp?lid=2 Our Address: 2118 Wilshire Blvd., Box 1008, Santa Monica, CA 90403-5784 From jqb7 <@t> cdc.gov Thu Nov 6 04:41:19 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Nov 6 05:05:40 2008 Subject: [Histonet] 200 proof alcohol? In-Reply-To: <51185D01EA0E4817A796D117CDE7D1BA@yourxhtr8hvc4p> References: <51185D01EA0E4817A796D117CDE7D1BA@yourxhtr8hvc4p> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C47C@LTA3VS011.ees.hhs.gov> I like mine with blue-cheese olives but to each his/her own. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, November 05, 2008 7:23 PM To: Caroline Bass; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] 200 proof alcohol? 200 proof is 200 proof, can't get any purer than that. Of course, I would take the taste test first. Green olives stuffed with Feta cheese goes good. JTT ----- Original Message ----- From: "Caroline Bass" To: Sent: Wednesday, November 05, 2008 11:15 AM Subject: [Histonet] 200 proof alcohol? Hello, I?m trying to buy some alcohol for both histology and molecular biology protocols. But I?m having some difficult getting ?molecular biology grade? 100% ethanol. The hospital I work at sells 200 proof ethanol, but can?t say anything about the quality. Since it?s 200 proof, is that basically the same as molecular biology grade? How about histology grade? I seem to recall using hospital provided ethanol for molecular biology experiments in my postdoc, so I think it should be ok. Any advice is appreciated! Caroline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Nov 6 05:53:21 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Nov 6 05:53:24 2008 Subject: [Histonet] 200 proof alcohol? References: <51185D01EA0E4817A796D117CDE7D1BA@yourxhtr8hvc4p> <1CE1847DFEA0A647B1CCDE4108EA60A70208C47C@LTA3VS011.ees.hhs.gov> Message-ID: I'm allergic to blue cheese. ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Joe Nocito" ; "Caroline Bass" ; Sent: Thursday, November 06, 2008 4:41 AM Subject: RE: [Histonet] 200 proof alcohol? I like mine with blue-cheese olives but to each his/her own. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, November 05, 2008 7:23 PM To: Caroline Bass; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] 200 proof alcohol? 200 proof is 200 proof, can't get any purer than that. Of course, I would take the taste test first. Green olives stuffed with Feta cheese goes good. JTT ----- Original Message ----- From: "Caroline Bass" To: Sent: Wednesday, November 05, 2008 11:15 AM Subject: [Histonet] 200 proof alcohol? Hello, I?m trying to buy some alcohol for both histology and molecular biology protocols. But I?m having some difficult getting ?molecular biology grade? 100% ethanol. The hospital I work at sells 200 proof ethanol, but can?t say anything about the quality. Since it?s 200 proof, is that basically the same as molecular biology grade? How about histology grade? I seem to recall using hospital provided ethanol for molecular biology experiments in my postdoc, so I think it should be ok. Any advice is appreciated! Caroline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From emerald_lake77 <@t> yahoo.com Thu Nov 6 07:39:58 2008 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Thu Nov 6 07:40:02 2008 Subject: [Histonet] Equipment available to my lab... but what are they? Message-ID: <204197.75086.qm@web31704.mail.mud.yahoo.com> Hi all, ? We have some equipment in our company that is being transferred from one location to another.? Someone sent me photos of Leica equipment and I did a quick search to see what they are .... but I needed some clarification as to what people use them for, to see if it will fit my current lab and need: ? 1. Leica VT100E 2. Leica?SM2000R ? Does anyone use these??? What are some specific applications that make it different from a regular microtome or cryostat? ? Thanks ? Gustave ? ? From we3smitty <@t> yahoo.com Thu Nov 6 07:43:56 2008 From: we3smitty <@t> yahoo.com (angela smith) Date: Thu Nov 6 07:44:02 2008 Subject: [Histonet] histonet Message-ID: <73315.68963.qm@web62006.mail.re1.yahoo.com> I have asked to be removed from the list and I am still getting the emails. Please best advise. Angela From rjbuesa <@t> yahoo.com Thu Nov 6 08:11:35 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Nov 6 08:11:39 2008 Subject: [Histonet] histonet In-Reply-To: <73315.68963.qm@web62006.mail.re1.yahoo.com> Message-ID: <210235.84469.qm@web65701.mail.ac4.yahoo.com> Go to Histonet directly and remove yourself. Ren? J. --- On Thu, 11/6/08, angela smith wrote: From: angela smith Subject: [Histonet] histonet To: histonet@lists.utsouthwestern.edu Date: Thursday, November 6, 2008, 8:43 AM I have asked to be removed from the list and I am still getting the emails. Please best advise. Angela _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu Nov 6 09:15:39 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Nov 6 09:16:02 2008 Subject: [Histonet] President In-Reply-To: References: Message-ID: <4912C3CA.2B7F.00C9.0@geisinger.edu> not really...just want to keep this arena of scientific thinking sacred (and totally non-partisan)! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Joachim Hehl 11/5/2008 10:58 AM >>> Interesting, the histonet list seems to be occupied by ultraconservatives. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From gu.lang <@t> gmx.at Thu Nov 6 09:42:45 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Nov 6 09:43:15 2008 Subject: AW: [Histonet] Fatty tissue In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2705635AB4@hpes1.HealthPartners.int> References: <0E394B648E5284478A6CCB78E5AFDA2705635AB4@hpes1.HealthPartners.int> Message-ID: <3FBCD44126844AA68A61B26898ED4095@dielangs.at> We have made some changes to our regular VIP-protocol (13 hours): Increasing time in 100%ethanol, in shell sol (=Xylen-substitute) and paraffin to 16 hours. This led to an improvement of paraffin-infiltration and sectioning-qualitiy. Some blocks can be improved by letting them sit in melted paraffin for a few hours after the processing. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Webb, Dorothy L Gesendet: Mittwoch, 05. November 2008 21:22 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Fatty tissue I would like to know how other labs are processing "fatty" tissue, especially breast for optimal processing? We have been doing a few "reprocessing" of these specimens and my pathologist feels this should not be happening! Yes, the PA's are, at times, cutting the sections larger than 3 mm thick, but, at times the tumor is ingrained in the fatty area and they need to obtain as much of the perimeter as they can. Does anyone agitate or heat fixate their specimens prior to processing? Would appreciate any feedback on this age old dilemma. Also, I only heard back from one person on the recycling issue. Any input would be appreciated as to savings incurred and a still vs. recycler. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mari.ann.mailhiot <@t> leica-microsystems.com Thu Nov 6 09:51:00 2008 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Thu Nov 6 09:51:25 2008 Subject: [Histonet] Equipment available to my lab... but what are they? In-Reply-To: <204197.75086.qm@web31704.mail.mud.yahoo.com> Message-ID: Gustave The VT1000E is a vibrating microtome. It is used to cut fresh brain tissue. The specimen is usually remove and place on the microtome on a special disc. The disc sits in a buffer tray that has chilled or room temp saline. The unit has specific applications in neurology and is a vibrating blade microtome, designed specifically to meet the highly sophisticated sectioning requirements in the fields of neurophysiology, neuropathology and experimental (brain) research and some industrial applications related to structural analysis of foam and other very soft materials. The SM2000R is a sliding microtome and can cut paraffin blocks or fresh specimens surrounded in dry ice. The Leica SM2000 R sliding microtome with low-maintenance cross-roller-bearing guide-ways is designed to section paraffin-embedded samples in routine histopathology laboratories. The SM2000 R can also be used for the specific industrial applications of foam and wood sectioning. The easy-to-use knife holder is designed to accommodate both standard knives and disposable blades. The instrument is available fully equipped to meet most of today's sliding microtome sectioning requirements and can be customized to meet more special applications. If you have any questions don't hesitate to call. Best regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com GT Hebert To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Equipment available to my lab... but what are they? 11/06/2008 07:39 AM Please respond to emerald_lake77@ya hoo.com Hi all, We have some equipment in our company that is being transferred from one location to another.? Someone sent me photos of Leica equipment and I did a quick search to see what they are .... but I needed some clarification as to what people use them for, to see if it will fit my current lab and need: 1. Leica VT100E 2. Leica?SM2000R Does anyone use these?? What are some specific applications that make it different from a regular microtome or cryostat? Thanks Gustave _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From tbraud <@t> holyredeemer.com Thu Nov 6 10:56:29 2008 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Nov 6 10:56:36 2008 Subject: [Histonet] Pathologist workload Message-ID: I've been trying to research figures on workloads for pathologists and there is almost no published for US data out there. There is actually more on workload for Histotechs. If you care to participate, please email me with as much of the following information as you can. Thank you, Terri Number of Pathologists: Number of PAs: Surgical Cases per year: Surgical Blocks per year: Surgical Slides (incl all IHC, Specials) per year: NOTE: Please include anything that is not a cytology in the above figures, ie. bone marrows, neuropath, etc Do the Histotechs "gross" in any tissues? YES or NO NOTE: This includes the processing or transfer of small biopsies Do the Pathologists have teaching responsibilities: YES or NO NOTE: If yes, then do Residents have the primary responsibility for gross: YES or NO Non-Gyn Cyto cases per year: Gyn cases per year: FNA cases per year: Number of cytotechs: Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From cbarone <@t> NEMOURS.ORG Thu Nov 6 11:00:02 2008 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Thu Nov 6 11:00:18 2008 Subject: [Histonet] ??? once again..is anyone out there? Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE801471892@wlmmsx01.nemours.org> ??? Any recommendations for either of these: 1.Processing and cutting of longitudnal sections of PVFD fibers...sometimes called "hollow fibers". We are having issues keeping the sections on the slides, no matter what slides we use/ or coat? 2.Di I staining...who has a histology protocol....for Di I staining? I know there are researchers out there somewhere...???? You know you love this wierd stuff! From akbitting <@t> geisinger.edu Thu Nov 6 11:03:18 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Nov 6 11:03:36 2008 Subject: AW: [Histonet] Fatty tissue In-Reply-To: <3FBCD44126844AA68A61B26898ED4095@dielangs.at> References: <0E394B648E5284478A6CCB78E5AFDA2705635AB4@hpes1.HealthPartners.int> <3FBCD44126844AA68A61B26898ED4095@dielangs.at> Message-ID: <4912DD06.2B7F.00C9.0@geisinger.edu> We've done the same. Additional time in Xylene and paraffin made a huge difference. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Gudrun Lang" 11/6/2008 10:42 AM >>> We have made some changes to our regular VIP-protocol (13 hours): Increasing time in 100%ethanol, in shell sol (=Xylen-substitute) and paraffin to 16 hours. This led to an improvement of paraffin-infiltration and sectioning-qualitiy. Some blocks can be improved by letting them sit in melted paraffin for a few hours after the processing. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Webb, Dorothy L Gesendet: Mittwoch, 05. November 2008 21:22 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Fatty tissue I would like to know how other labs are processing "fatty" tissue, especially breast for optimal processing? We have been doing a few "reprocessing" of these specimens and my pathologist feels this should not be happening! Yes, the PA's are, at times, cutting the sections larger than 3 mm thick, but, at times the tumor is ingrained in the fatty area and they need to obtain as much of the perimeter as they can. Does anyone agitate or heat fixate their specimens prior to processing? Would appreciate any feedback on this age old dilemma. Also, I only heard back from one person on the recycling issue. Any input would be appreciated as to savings incurred and a still vs. recycler. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From gcostello <@t> mdanderson.org Thu Nov 6 11:24:48 2008 From: gcostello <@t> mdanderson.org (Costello,Gerald C) Date: Thu Nov 6 11:25:08 2008 Subject: [Histonet] Remove me Message-ID: <9D68F7C8883263428DA29BBA25D60BFE15B2B8E74E@DCPWVMBXC0VS3.mdanderson.edu> Please remove me from the mailing list. NOW Thank You From Janet.Bonner <@t> FLHOSP.ORG Thu Nov 6 11:24:35 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Thu Nov 6 11:28:42 2008 Subject: [Histonet] ??? once again..is anyone out there? References: <37E4BAC017F57141AF64FAA5AEB04CE801471892@wlmmsx01.nemours.org> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2813@fhosxchmb006.ADVENTISTCORP.NET> Try staining by coverslipping the freshly sectioned tissue using water and a glass coverslip. Put the slide edge down on a towelette and apply the stain with a dropper to the top edge of the slide. The stain will spread under the coverslip as the water escapes into the absorptive towelette underneath. Wash the same way and move on to the next reagent. Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org 407-646-7559 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Barone, Carol Sent: Thu 11/6/2008 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ??? once again..is anyone out there? ??? Any recommendations for either of these: 1.Processing and cutting of longitudnal sections of PVFD fibers...sometimes called "hollow fibers". We are having issues keeping the sections on the slides, no matter what slides we use/ or coat? 2.Di I staining...who has a histology protocol....for Di I staining? I know there are researchers out there somewhere...???? You know you love this wierd stuff! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Terry.Marshall <@t> rothgen.nhs.uk Thu Nov 6 11:33:56 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Nov 6 11:34:02 2008 Subject: [Histonet] Remove me References: <9D68F7C8883263428DA29BBA25D60BFE15B2B8E74E@DCPWVMBXC0VS3.mdanderson.edu> Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F589@TRFT-EX01.xRothGen.nhs.uk> Now that, is just plain rude. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Costello,Gerald C Sent: 06 November 2008 17:25 To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Remove me Please remove me from the mailing list. NOW Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From emerald_lake77 <@t> yahoo.com Thu Nov 6 11:36:20 2008 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Thu Nov 6 11:36:24 2008 Subject: [Histonet] Equipment available to my lab... but what are they? In-Reply-To: Message-ID: <634695.62152.qm@web31708.mail.mud.yahoo.com> Thank you all for your insightful and helpful information. ? Gustave ---------------------------------------------------------------------------- The VT1000E is a vibrating microtome. It is used to cut fresh brain tissue. The specimen is usually remove and place on the microtome on a special disc. The disc sits in a buffer tray that has chilled or room temp saline. The unit has specific applications in neurology and is a vibrating blade microtome, designed specifically to meet the highly sophisticated sectioning requirements in the fields of neurophysiology, neuropathology and experimental (brain) research and some industrial applications related to structural analysis of foam and other very soft materials. The SM2000R is a sliding microtome and can cut paraffin blocks or fresh specimens surrounded in dry ice. The Leica SM2000 R sliding microtome with low-maintenance cross-roller-bearing guide-ways is designed to section paraffin-embedded samples in routine histopathology laboratories. The SM2000 R can also be used for the specific industrial applications of foam and wood sectioning. The easy-to-use knife holder is designed to accommodate both standard knives and disposable blades. The instrument is available fully equipped to meet most of today's sliding microtome sectioning requirements and can be customized to meet more special applications. From ljones <@t> pathology.umsmed.edu Thu Nov 6 11:37:04 2008 From: ljones <@t> pathology.umsmed.edu (Linda Jones) Date: Thu Nov 6 11:37:32 2008 Subject: [Histonet] Remove me Message-ID: <4912D6E0020000440002D4EB@GWIA1.umsmed.edu> Linda Harper-Jones BS.,HT/ HTL(ASCP) University of Mississippi Medical Center Department of Pathology Chief Histotechnologist Supervisor Telephone (601) 984-1576 Fax (601) 984-4968 ljones@pathology.umsmed.edu The information contained in this email is confidential. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From slappycraw <@t> yahoo.com Thu Nov 6 11:41:53 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Thu Nov 6 11:41:58 2008 Subject: [Histonet] IHC for helicobacter bili in mouse gut? Message-ID: <577159.84170.qm@web53612.mail.re2.yahoo.com> Any info would be appreciated. Thanks in advance. ? Larry A. Woody Seattle, Wa. From histotechkb <@t> gmail.com Thu Nov 6 11:45:14 2008 From: histotechkb <@t> gmail.com (Kristen Yaros) Date: Thu Nov 6 11:45:23 2008 Subject: [Histonet] Remove me In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F589@TRFT-EX01.xRothGen.nhs.uk> References: <9D68F7C8883263428DA29BBA25D60BFE15B2B8E74E@DCPWVMBXC0VS3.mdanderson.edu> <5C0BED61F529364E86309CADEA63FEF20163F589@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <667c97ab0811060945x7680ae72x8307dc1f60e27ec2@mail.gmail.com> No Kidding! Gerald (and others), you might find it more useful to go here: http://lists.utsouthwestern.edu/mailman/listinfo/histonet and follow the instructions for unsubscribing at the bottom of the page. Kristen On 11/6/08, Marshall Terry Dr, Consultant Histopathologist < Terry.Marshall@rothgen.nhs.uk> wrote: > > Now that, is just plain rude. > > Terry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Costello,Gerald C > Sent: 06 November 2008 17:25 > To: 'Histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Remove me > > Please remove me from the mailing list. NOW Thank You > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Kristen Yaros, HT (ASCP)CM Histotechnology Society of Delaware Correspondence Secretary histotechkb@gmail.com From ian.montgomery <@t> bio.gla.ac.uk Thu Nov 6 11:47:15 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Nov 6 11:49:19 2008 Subject: FW: [Histonet] Remove me Message-ID: <30529BC228AC4005A8D771B85D58DDAE@IBLS.GLA.AC.UK> Was thinking the same. Why will subscribers not remember the simple rule, subject line - unsubscribe. It's easy and always works. Grumpy rave over for the day, well, for the moment. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 06 November 2008 17:34 To: Costello,Gerald C; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Remove me Now that, is just plain rude. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Costello,Gerald C Sent: 06 November 2008 17:25 To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Remove me Please remove me from the mailing list. NOW Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Thu Nov 6 11:49:29 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Thu Nov 6 11:49:50 2008 Subject: [Histonet] Remove me In-Reply-To: <667c97ab0811060945x7680ae72x8307dc1f60e27ec2@mail.gmail.com> References: <9D68F7C8883263428DA29BBA25D60BFE15B2B8E74E@DCPWVMBXC0VS3.mdanderson.edu> <5C0BED61F529364E86309CADEA63FEF20163F589@TRFT-EX01.xRothGen.nhs.uk> <667c97ab0811060945x7680ae72x8307dc1f60e27ec2@mail.gmail.com> Message-ID: <2A582E8156B45F468A62D1F1D20AF083426B63@EX-BE08.ohsu.edu> Kristen, Thank you, thank you...of all the times people are instructed to do this, others that want to unsubscribing go and request us to remove them. Robyn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kristen Yaros Sent: Thursday, November 06, 2008 9:45 AM To: Marshall Terry Dr, Consultant Histopathologist Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Remove me No Kidding! Gerald (and others), you might find it more useful to go here: http://lists.utsouthwestern.edu/mailman/listinfo/histonet and follow the instructions for unsubscribing at the bottom of the page. Kristen On 11/6/08, Marshall Terry Dr, Consultant Histopathologist < Terry.Marshall@rothgen.nhs.uk> wrote: > > Now that, is just plain rude. > > Terry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Costello,Gerald C > Sent: 06 November 2008 17:25 > To: 'Histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Remove me > > Please remove me from the mailing list. NOW Thank You > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Kristen Yaros, HT (ASCP)CM Histotechnology Society of Delaware Correspondence Secretary histotechkb@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu Nov 6 11:51:01 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu Nov 6 11:51:06 2008 Subject: [Histonet] IHC for helicobacter bili in mouse gut? In-Reply-To: <577159.84170.qm@web53612.mail.re2.yahoo.com> Message-ID: We use VMS's H. Pylori on Ventana instruments (BMK, XT and NexES) with great results. If you have VMS instruments, let me know and I'll send you our protocols. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Larry Woody Sent: Thursday, November 06, 2008 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC for helicobacter bili in mouse gut? Any info would be appreciated. Thanks in advance. ? Larry A. Woody Seattle, Wa. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Nov 6 10:54:05 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Nov 6 12:04:00 2008 Subject: [Histonet] President In-Reply-To: <4912C3CA.2B7F.00C9.0@geisinger.edu> References: <4912C3CA.2B7F.00C9.0@geisinger.edu> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C48E@LTA3VS011.ees.hhs.gov> Thank you Angela! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, November 06, 2008 10:16 AM To: histonet@lists.utsouthwestern.edu; Joachim Hehl Subject: Re: [Histonet] President not really...just want to keep this arena of scientific thinking sacred (and totally non-partisan)! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> Joachim Hehl 11/5/2008 10:58 AM >>> Interesting, the histonet list seems to be occupied by ultraconservatives. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From patricia.royal <@t> roche.com Thu Nov 6 12:18:09 2008 From: patricia.royal <@t> roche.com (Royal, Patricia) Date: Thu Nov 6 12:18:20 2008 Subject: [Histonet] (no subject) Message-ID: PLEASE REMOVE ME FROM THE LIST. Patricia Royal From kdboydhisto <@t> yahoo.com Thu Nov 6 12:25:43 2008 From: kdboydhisto <@t> yahoo.com (KELLY BOYD) Date: Thu Nov 6 12:25:47 2008 Subject: [Histonet] embedding Message-ID: <934704.35648.qm@web58603.mail.re3.yahoo.com> Just trying to get some feedback on how much time (estimated) it should take an average histotech with experience to embed 150 shave biopsies. Let us just say the majority have multiple pieces to be embeded on edge. Any clue? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com? ? Tele (252)-830-6866 Cell? (252)-943-9527 Fax? (252)-830-0032 ? ? From maxim_71 <@t> mail.ru Thu Nov 6 12:50:34 2008 From: maxim_71 <@t> mail.ru (maxim_71@mail.ru) Date: Thu Nov 6 12:53:07 2008 Subject: [Histonet] Fatty tissue Message-ID: <829987455.20081106215034@mail.ru> When we switched on isopropanol as dehydratant and mineral oil as clearant we forgot all our problem with processing for fatty tissues and many other difficult type of tissues. Thickness no more than 3 mm is main requirement for processing such type tissues. If you need details, please let me know. Maxim Peshkov Russia, Taganrog. mailto:maxim_71@mail.ru From Jackie.O'Connor <@t> abbott.com Thu Nov 6 12:58:03 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Nov 6 12:58:23 2008 Subject: [Histonet] embedding In-Reply-To: <934704.35648.qm@web58603.mail.re3.yahoo.com> Message-ID: 2-3 hours. KELLY BOYD Sent by: histonet-bounces@lists.utsouthwestern.edu 11/06/2008 12:25 PM Please respond to kdboydhisto@yahoo.com To histonet cc Subject [Histonet] embedding Just trying to get some feedback on how much time (estimated) it should take an average histotech with experience to embed 150 shave biopsies. Let us just say the majority have multiple pieces to be embeded on edge. Any clue? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stephanie.d.rivera <@t> gsk.com Thu Nov 6 13:01:19 2008 From: stephanie.d.rivera <@t> gsk.com (stephanie.d.rivera@gsk.com) Date: Thu Nov 6 13:02:09 2008 Subject: [Histonet] embedding In-Reply-To: <934704.35648.qm@web58603.mail.re3.yahoo.com> Message-ID: Hi Kelly, I would say at least 2.5 - 3 hrs. Estimating embedding approx. 50 blocks/hr. That is reasonable. That doesn't include scraping and organizing the blocks. Stephanie D. Rivera, B.S., HT(ASCP) Scientist Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 "KELLY BOYD" Sent by: histonet-bounces@lists.utsouthwestern.edu 06-Nov-2008 13:25 Please respond to kdboydhisto@yahoo.com To "histonet" cc Subject [Histonet] embedding Just trying to get some feedback on how much time (estimated) it should take an average histotech with experience to embed 150 shave biopsies. Let us just say the majority have multiple pieces to be embeded on edge. Any clue? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lchen <@t> mednet.ucla.edu Thu Nov 6 13:14:39 2008 From: lchen <@t> mednet.ucla.edu (Leslie Chen) Date: Thu Nov 6 13:14:44 2008 Subject: [Histonet] 200 proof Message-ID: <361429220811061114m84ce24ah70ec8c3ad14d0035@mail.gmail.com> Our MBG Ethanol is made by Gold Shield Chemical Co in Hayward, CA. It's called Rossville Gold Shield Ethyl Alcohol Leslie From froyer <@t> bitstream.net Thu Nov 6 13:14:29 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Nov 6 13:14:56 2008 Subject: [Histonet] INSTRUCTIONS: To Those Who Wish To be Removed From This LIST... In-Reply-To: <30529BC228AC4005A8D771B85D58DDAE@IBLS.GLA.AC.UK> References: <30529BC228AC4005A8D771B85D58DDAE@IBLS.GLA.AC.UK> Message-ID: <942D649BB02949728849D9DAA37C337F@Ford> Okay, I'll step forward with this... JUST A REMINDER TO ALL: When this list was in its early days the procedure to "Subscribe" and/or "Unsubscribe" was very simple. All you had to do was send an email to the list address with one of the two afore mentioned words listed in the "SUBJECT" line of the email. No words were required to be placed in the body of the message. (I think that is what Ian is referring to but, alas Ian, this no longer works nor is the proper procedure). Now... To Subscribe, Unsubscribe, put mail on "Hold", or to change ANYTHING about your subscription information, you must independently go to: http://lists.utsouthwestern.edu/mailman/listinfo/histonet ...and follow the instructions on the printed screen. Note also that you must do this from the same computer that you original used to start your subscription. This procedure was provided to all when you first subscribed to the HistoNet, but like so many other things on email, I'm sure that it was deleted or filed in an obscure file long ago and longer ago forgotten. Posting your wishes on this email server (histonet@lists.utsouthwestern.edu) accomplishes absolutely nothing, and by getting frustrated and angry at this non-accomplishment, and posting it repeatedly on the HistoNet, does nothing more than to embarrass yourself in the front of over 3,300 subscribers who read it. So please. if you wish to no longer receive emails from the HistoNet, go to http://lists.utsouthwestern.edu/mailman/listinfo/histonet and Unsubscribe yourself. This is your responsibility, NOT the responsibility of the List owner or List master, or any of the thousands of subscribers who read your attempts. Once accomplished, you will find that your level of stress will decrease markedly. Best of luck on your quest and have a gentle day. ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. Golden Valley, MN 55427-3601 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 06 November 2008 17:34 To: Costello,Gerald C; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Remove me Now that, is just plain rude. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Costello,Gerald C Sent: 06 November 2008 17:25 To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Remove me Please remove me from the mailing list. NOW Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Thu Nov 6 13:17:06 2008 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Thu Nov 6 13:21:31 2008 Subject: [Histonet] embedding In-Reply-To: <934704.35648.qm@web58603.mail.re3.yahoo.com> References: <934704.35648.qm@web58603.mail.re3.yahoo.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D2D14DB45@LRGHEXVS1.practice.lrgh.org> I would say 2 to 4 hours, depending on how they are submitted. Were biopsy bags or foam pads used?, size of pcs. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of KELLY BOYD [kdboydhisto@yahoo.com] Sent: Thursday, November 06, 2008 1:25 PM To: histonet Subject: [Histonet] embedding Just trying to get some feedback on how much time (estimated) it should take an average histotech with experience to embed 150 shave biopsies. Let us just say the majority have multiple pieces to be embeded on edge. Any clue? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From carrolpb <@t> umdnj.edu Thu Nov 6 11:37:44 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Thu Nov 6 13:37:15 2008 Subject: [Histonet] Remove me In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F589@TRFT-EX01.xRothGen.nhs.uk> References: <9D68F7C8883263428DA29BBA25D60BFE15B2B8E74E@DCPWVMBXC0VS3.mdanderson.edu> <5C0BED61F529364E86309CADEA63FEF20163F589@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <49132B68.3000804@umdnj.edu> ...especially when one can simply click the link at the bottom of EVERY mail from this list and take care one's subscription one's self! Marshall Terry Dr, Consultant Histopathologist wrote: > Now that, is just plain rude. > > Terry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Costello,Gerald C > Sent: 06 November 2008 17:25 > To: 'Histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Remove me > > Please remove me from the mailing list. NOW Thank You > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From joelleweaver <@t> hotmail.com Thu Nov 6 14:08:26 2008 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Nov 6 14:08:33 2008 Subject: [Histonet] embedding In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638631D2D14DB45@LRGHEXVS1.practice.lrgh.org> References: <934704.35648.qm@web58603.mail.re3.yahoo.com> <38667E7FB77ECD4E91BFAEB8D98638631D2D14DB45@LRGHEXVS1.practice.lrgh.org> Message-ID: I have seen use of the baseline of 40 blocks/hour, or 0.66min/block,- to include, scraping, checking off and putting in order. In my experience this amount is pretty easy to do even with skins, or multiple piece biopsies, papers, sponges etc, Joelle Weaver HTL (ASCP)> From: tpodawiltz@lrgh.org> To: kdboydhisto@yahoo.com; Histonet@lists.utsouthwestern.edu> Date: Thu, 6 Nov 2008 14:17:06 -0500> Subject: RE: [Histonet] embedding> CC: > > I would say 2 to 4 hours, depending on how they are submitted. Were biopsy bags or foam pads used?, size of pcs.> > > Tom Podawiltz, HT (ASCP)> Histology Section Head/Laboratory Safety Officer> LRGHealthcare> 603-524-3211 ext: 3220> ________________________________________> From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of KELLY BOYD [kdboydhisto@yahoo.com]> Sent: Thursday, November 06, 2008 1:25 PM> To: histonet> Subject: [Histonet] embedding> > Just trying to get some feedback on how much time (estimated) it should take an average histotech with experience to embed 150 shave biopsies. Let us just say the majority have multiple pieces to be embeded on edge. Any clue?> > > Kelly D. Boyd, BS, HTL (ASCP)> Lab Manager> Harris Histology Services> 2025 Eastgate Dr. Ste. F> Greenville, NC 27858> www.harrishisto.com> > Tele (252)-830-6866> Cell (252)-943-9527> Fax (252)-830-0032> > > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Stay up to date on your PC, the Web, and your mobile phone with Windows Live http://clk.atdmt.com/MRT/go/119462413/direct/01/ From suhyoung.jeong <@t> gmail.com Thu Nov 6 14:36:45 2008 From: suhyoung.jeong <@t> gmail.com (Suhyoung Jeong) Date: Thu Nov 6 14:36:50 2008 Subject: [Histonet] Mouse antibody to fibroblast without human crossreactivity? Message-ID: <450012a20811061236h6daa8b2bs7da53c0e7c146d21@mail.gmail.com> Hello everyone, I am looking for a primary antibody that will recognize a protein in mouse fibroblast without crossreactivity to human. If you have any experience in this matter, please kindly let me know. Thank you in advance Suh From epearlstein <@t> hotmail.com Thu Nov 6 15:35:11 2008 From: epearlstein <@t> hotmail.com (Ellen Pearlstein) Date: Thu Nov 6 15:35:15 2008 Subject: [Histonet] REMOVE FROM LIST Message-ID: please remove me from the list. _________________________________________________________________ Color coding for safety: Windows Live Hotmail alerts you to suspicious email. http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_safety_112008 From froyer <@t> bitstream.net Thu Nov 6 16:18:17 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Nov 6 16:18:43 2008 Subject: [Histonet] REMOVE FROM LIST In-Reply-To: References: Message-ID: <8724287FE21E4440BB2C1032452A1767@Ford> You have GOT to be kidding! ... someone's just has to be pulling our leg. ;-) (If not. Ellen, click on the link that is shown at the very bottom of this page, and read what it says when you get to the web page that it is linked to. I wish you the very best, and good luck to you.) Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen Pearlstein Sent: Thursday, November 06, 2008 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] REMOVE FROM LIST please remove me from the list. _________________________________________________________________ Color coding for safety: Windows Live Hotmail alerts you to suspicious email. http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_safety_ 112008_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Thu Nov 6 16:25:11 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Nov 6 16:25:16 2008 Subject: [Histonet] REMOVE FROM LIST In-Reply-To: <8724287FE21E4440BB2C1032452A1767@Ford> References: <8724287FE21E4440BB2C1032452A1767@Ford> Message-ID: <49136EC7.3030202@pathology.washington.edu> Ford, I hope you took your blood pressure medication today? Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Ford Royer wrote: > You have GOT to be kidding! ... someone's just has to be pulling our leg. > > ;-) > > > > (If not. Ellen, click on the link that is shown at the very bottom of this > page, and read what it says when you get to the web page that it is linked > to. I wish you the very best, and good luck to you.) > > > > Ford M. Royer, MT(ASCP) > > Minnesota Medical, Inc. > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Pearlstein > Sent: Thursday, November 06, 2008 3:35 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] REMOVE FROM LIST > > > > > > please remove me from the list. > > > > _________________________________________________________________ > > Color coding for safety: Windows Live Hotmail alerts you to suspicious > email. > > http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_safety_ > 112008_______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jnocito <@t> satx.rr.com Thu Nov 6 17:16:01 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Nov 6 17:16:10 2008 Subject: [Histonet] REMOVE FROM LIST References: <8724287FE21E4440BB2C1032452A1767@Ford> <49136EC7.3030202@pathology.washington.edu> Message-ID: <2F76909274C243139D4BEE5A7B61BED0@yourxhtr8hvc4p> blood pressure medicine, geez, I want some of that Gold Seal alcohol. And I don't want to unsubscribe (did I spell that right?) JTT ----- Original Message ----- From: "Victor Tobias" To: "Ford Royer" Cc: Sent: Thursday, November 06, 2008 4:25 PM Subject: Re: [Histonet] REMOVE FROM LIST > Ford, > > I hope you took your blood pressure medication today? > > Victor > > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use of > the intended recipients. If you are not the intended recipient, or if the > message has been addressed to you in error, do not read, disclose, > reproduce, distribute, disseminate or otherwise use this transmission. > Instead, please notify the sender by reply e-mail, and then destroy all > copies of the message and any attachments. > > > > Ford Royer wrote: >> You have GOT to be kidding! ... someone's just has to be pulling our leg. >> ;-) >> >> >> (If not. Ellen, click on the link that is shown at the very bottom of >> this >> page, and read what it says when you get to the web page that it is >> linked >> to. I wish you the very best, and good luck to you.) >> >> Ford M. Royer, MT(ASCP) >> >> Minnesota Medical, Inc. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen >> Pearlstein >> Sent: Thursday, November 06, 2008 3:35 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] REMOVE FROM LIST >> >> >> >> please remove me from the list. >> >> >> _________________________________________________________________ >> >> Color coding for safety: Windows Live Hotmail alerts you to suspicious >> email. >> >> http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_safety_ >> 112008_______________________________________________ >> >> Histonet mailing list >> >> Histonet@lists.utsouthwestern.edu >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Thu Nov 6 18:14:41 2008 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Thu Nov 6 18:14:45 2008 Subject: [Histonet] Fatty tissue In-Reply-To: References: <0E394B648E5284478A6CCB78E5AFDA2705635AB4@hpes1.HealthPartners.int> Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCF01F3@IRMEXCH01.irm.inhs.org> Hello, Are people microwave fixing breast tissues? Just wondering as/does this fly in the face of the "6-48" hour fixation requirement for any breast tissue that is to have Her2-neu testing? Thanks, Greg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Wednesday, November 05, 2008 2:43 PM To: Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fatty tissue I would suggest microwaving the tissue blocks in formalin for at least 1 hour (longer is better) up to a temperature of 45oC (you might even consider a higher temp maybe up to 55oC??) We use this with our placenta blocks and the results were dramatically improved (35% poorly processed to less than 1% after microwaving). We have a Mega TT Microwave oven Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, 6 November 2008 7:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fatty tissue I would like to know how other labs are processing "fatty" tissue, especially breast for optimal processing? We have been doing a few "reprocessing" of these specimens and my pathologist feels this should not be happening! Yes, the PA's are, at times, cutting the sections larger than 3 mm thick, but, at times the tumor is ingrained in the fatty area and they need to obtain as much of the perimeter as they can. Does anyone agitate or heat fixate their specimens prior to processing? Would appreciate any feedback on this age old dilemma. Also, I only heard back from one person on the recycling issue. Any input would be appreciated as to savings incurred and a still vs. recycler. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Nov 6 19:49:05 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Nov 6 19:49:19 2008 Subject: [Histonet] Fatty tissue In-Reply-To: <6BB8BC4519AAB844B174FC739A679BBCCF01F3@IRMEXCH01.irm.inhs.org> Message-ID: Thank heaven's for working in a Children's Hospital. I don't have to woory about her2 nor her3,4 or any others!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Luck, Greg D. [mailto:LuckG@empirehealth.org] Sent: Friday, 7 November 2008 11:15 AM To: Tony Henwood; Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fatty tissue Hello, Are people microwave fixing breast tissues? Just wondering as/does this fly in the face of the "6-48" hour fixation requirement for any breast tissue that is to have Her2-neu testing? Thanks, Greg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Wednesday, November 05, 2008 2:43 PM To: Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fatty tissue I would suggest microwaving the tissue blocks in formalin for at least 1 hour (longer is better) up to a temperature of 45oC (you might even consider a higher temp maybe up to 55oC??) We use this with our placenta blocks and the results were dramatically improved (35% poorly processed to less than 1% after microwaving). We have a Mega TT Microwave oven Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, 6 November 2008 7:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fatty tissue I would like to know how other labs are processing "fatty" tissue, especially breast for optimal processing? We have been doing a few "reprocessing" of these specimens and my pathologist feels this should not be happening! Yes, the PA's are, at times, cutting the sections larger than 3 mm thick, but, at times the tumor is ingrained in the fatty area and they need to obtain as much of the perimeter as they can. Does anyone agitate or heat fixate their specimens prior to processing? Would appreciate any feedback on this age old dilemma. Also, I only heard back from one person on the recycling issue. Any input would be appreciated as to savings incurred and a still vs. recycler. ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From pieronelva01 <@t> bigpond.com Fri Nov 7 03:15:11 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Fri Nov 7 03:15:25 2008 Subject: [Histonet] CD4 on Ventana References: <30529BC228AC4005A8D771B85D58DDAE@IBLS.GLA.AC.UK> <942D649BB02949728849D9DAA37C337F@Ford> Message-ID: <2D0669F5E5EA4786A17AE07D652FFF51@pentium4> Dear Histonetters I'm having a lot of trouble getting CD4 to stain FFPE tissue using the Ventana Benchmark XT. Any advice about which antibody and protocol suggestions would be greatly appreciated. Piero Nelva Anatomical Pathology Monash Medical Centre Australia From ian.montgomery <@t> bio.gla.ac.uk Fri Nov 7 05:45:27 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Nov 7 05:47:52 2008 Subject: [Histonet] Advice Message-ID: Currently, in my tissue log book I have a column headed, FIXATION NUMBER. Here I give every new piece of tissue I receive a sequential number, year and the next number. Now that my brief includes human anatomy I think that this simple term might not be enough. The tissue will still have the same number; it's just that I think a new column heading is needed. What I'm looking for is the term widely used in labs that would cover everything from pieces of research mammalian tissue to human material. Isn't it always the simplest things that really tax the brain? Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From ian.montgomery <@t> bio.gla.ac.uk Fri Nov 7 05:54:48 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Nov 7 05:55:15 2008 Subject: [Histonet] All is revealed. Message-ID: Ford, Many thanks and me thinking it was simply, unscibe. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From v.neubert <@t> web.de Fri Nov 7 07:41:54 2008 From: v.neubert <@t> web.de (v.neubert@web.de) Date: Fri Nov 7 07:42:01 2008 Subject: [Histonet] Gram stain on tissue Message-ID: <393380075@web.de> Hello! To introduce myself: I've been reading this list for the last ~350 mails. I am a technical assistent, working in vet histo. I am quite inexperienced with staining, and one task is to get Gram staining to work. Right now, I've been trying to get results for 2! days, I tried about 10 different ways. If someone could share a method which has proven to be working, I'd be very happy. Reagents available to work with right now: xylene, acetone, picric acid, carbol fuchsin, basic fuchsin, safranine, fuchsin 1:10. I'd be glad if I had not to order any more extra reagents. Thank you for replying. Greets from Germany, Valentin __________________________________________________________________ "Run, Fatboy, Run" sowie "Rails & Ties" kostenlos anschauen! Blockbuster-Gutscheine sichern unter http://www.blockbuster.web.de From talulahgosh <@t> gmail.com Fri Nov 7 07:56:01 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Nov 7 07:56:06 2008 Subject: [Histonet] 200 proof alcohol? In-Reply-To: References: <51185D01EA0E4817A796D117CDE7D1BA@yourxhtr8hvc4p> <1CE1847DFEA0A647B1CCDE4108EA60A70208C47C@LTA3VS011.ees.hhs.gov> Message-ID: Our alcohol (95 to 100%) is sold by the university only, we don't get a choice. Hopefully if I ever need to use molecular biology grade, the stuff they sell will be good enough. Is it regulated in other universities? I have no idea why they do this, unless they think we're going to have parties. Actually, in PA you can't buy grain liquor, so maybe that's why. Emily -- "You would know her for all the things she was...a woman who knew her way in and out of every new book without being singed, pinched, bumped or tickled by any line or chapter." John O'Hara, Appointment in Samarra From rjbuesa <@t> yahoo.com Fri Nov 7 07:56:21 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 7 07:56:26 2008 Subject: [Histonet] Fatty tissue In-Reply-To: <6BB8BC4519AAB844B174FC739A679BBCCF01F3@IRMEXCH01.irm.inhs.org> Message-ID: <719963.36031.qm@web65702.mail.ac4.yahoo.com> The MW irradiation will NOT accelerate formalin cross linking; essentially MW irradiation will have a "cooking" effect on tissue, that is why the "6-48" rule was?issued by ASCO-CAP After MW irradiation the tissues that go into dehydration will be only partially fixed with formalin and will end being fixed by the alcohols in a "2 fixation" process with unknown consequences for the procedures that follow.The anecdotal "successful" use of MW for tissue fixation are just that, anecdotal, Ren? J. --- On Thu, 11/6/08, Luck, Greg D. wrote: From: Luck, Greg D. Subject: RE: [Histonet] Fatty tissue To: "Tony Henwood" , "Webb, Dorothy L" , histonet@lists.utsouthwestern.edu Date: Thursday, November 6, 2008, 7:14 PM Hello, Are people microwave fixing breast tissues? Just wondering as/does this fly in the face of the "6-48" hour fixation requirement for any breast tissue that is to have Her2-neu testing? Thanks, Greg From rjbuesa <@t> yahoo.com Fri Nov 7 08:18:29 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 7 08:18:33 2008 Subject: [Histonet] Gram stain on tissue In-Reply-To: <393380075@web.de> Message-ID: <52650.53923.qm@web65702.mail.ac4.yahoo.com> Valentin: Later in the day I will send you my procedure. It always worked very well for me. Ren? J. --- On Fri, 11/7/08, v.neubert@web.de wrote: From: v.neubert@web.de Subject: [Histonet] Gram stain on tissue To: histonet@lists.utsouthwestern.edu Date: Friday, November 7, 2008, 8:41 AM Hello! To introduce myself: I've been reading this list for the last ~350 mails. I am a technical assistent, working in vet histo. I am quite inexperienced with staining, and one task is to get Gram staining to work. Right now, I've been trying to get results for 2! days, I tried about 10 different ways. If someone could share a method which has proven to be working, I'd be very happy. Reagents available to work with right now: xylene, acetone, picric acid, carbol fuchsin, basic fuchsin, safranine, fuchsin 1:10. I'd be glad if I had not to order any more extra reagents. Thank you for replying. Greets from Germany, Valentin __________________________________________________________________ "Run, Fatboy, Run" sowie "Rails & Ties" kostenlos anschauen! Blockbuster-Gutscheine sichern unter http://www.blockbuster.web.de _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Fri Nov 7 08:29:10 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Fri Nov 7 08:29:19 2008 Subject: [Histonet] 200 proof alcohol? In-Reply-To: References: <51185D01EA0E4817A796D117CDE7D1BA@yourxhtr8hvc4p> <1CE1847DFEA0A647B1CCDE4108EA60A70208C47C@LTA3VS011.ees.hhs.gov> Message-ID: At our university in Buffalo, NY, we have to sign out the alcohol we purchase from the university (we could buy it from Sigma or other places, I suppose, but generally want to avoid the SH fee). I was told the State of NY required the signature...why? So if we're caught driving drunk they can link it to the alcohol we purchased through the university and diluted to have a party?? And the university would be held liable?? ;-) Merced --On Friday, November 07, 2008 8:56 AM -0500 Emily Sours wrote: > Our alcohol (95 to 100%) is sold by the university only, we don't get a > choice. Hopefully if I ever need to use molecular biology grade, the > stuff they sell will be good enough. Is it regulated in other > universities? I have no idea why they do this, unless they think we're > going to have parties. > Actually, in PA you can't buy grain liquor, so maybe that's why. > > Emily > -- > "You would know her for all the things she was...a woman who knew her way > in and out of every new book without being singed, pinched, bumped or > tickled by any line or chapter." > John O'Hara, Appointment in Samarra > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (pkgs) / 140 Farber Hall (letters) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 "Without my flaws I'm really very boring." - random internet blog commentator From LSebree <@t> uwhealth.org Fri Nov 7 08:36:05 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Fri Nov 7 08:36:11 2008 Subject: [Histonet] CD4 on Ventana In-Reply-To: <2D0669F5E5EA4786A17AE07D652FFF51@pentium4> Message-ID: Piero, We use C4d from American Research Products, Inc., cat. #: 12-50000 at 1:50. On the BenchMark XT we use mild CC1 for 32" at 42 degrees C with AB block. Hope this helps. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piero Nelva Sent: Friday, November 07, 2008 3:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD4 on Ventana Dear Histonetters I'm having a lot of trouble getting CD4 to stain FFPE tissue using the Ventana Benchmark XT. Any advice about which antibody and protocol suggestions would be greatly appreciated. Piero Nelva Anatomical Pathology Monash Medical Centre Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Fri Nov 7 08:45:05 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Fri Nov 7 08:45:15 2008 Subject: [Histonet] 200 proof alcohol? In-Reply-To: References: <51185D01EA0E4817A796D117CDE7D1BA@yourxhtr8hvc4p> <1CE1847DFEA0A647B1CCDE4108EA60A70208C47C@LTA3VS011.ees.hhs.gov> Message-ID: <10E29EE30C6C55FDC0274B9F@bchwxp2702.ad.med.buffalo.edu> Ahhh...they are federally regulated... --On Friday, November 07, 2008 9:29 AM -0500 Merced Leiker wrote: > At our university in Buffalo, NY, we have to sign out the alcohol we > purchase from the university (we could buy it from Sigma or other places, > I suppose, but generally want to avoid the SH fee). I was told the State > of NY required the signature...why? So if we're caught driving drunk they > can link it to the alcohol we purchased through the university and > diluted to have a party?? And the university would be held liable?? ;-) > > Merced > > --On Friday, November 07, 2008 8:56 AM -0500 Emily Sours > wrote: > >> Our alcohol (95 to 100%) is sold by the university only, we don't get a >> choice. Hopefully if I ever need to use molecular biology grade, the >> stuff they sell will be good enough. Is it regulated in other >> universities? I have no idea why they do this, unless they think we're >> going to have parties. >> Actually, in PA you can't buy grain liquor, so maybe that's why. >> >> Emily >> -- >> "You would know her for all the things she was...a woman who knew her way >> in and out of every new book without being singed, pinched, bumped or >> tickled by any line or chapter." >> John O'Hara, Appointment in Samarra >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician II > 354 BRB (pkgs) / 140 Farber Hall (letters) > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > "Without my flaws I'm really very boring." > - random internet blog commentator > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (pkgs) / 140 Farber Hall (letters) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 "Without my flaws I'm really very boring." - random internet blog commentator From v.neubert <@t> web.de Fri Nov 7 08:55:45 2008 From: v.neubert <@t> web.de (Valentin Neubert) Date: Fri Nov 7 08:55:54 2008 Subject: [Histonet] Gram stain on tissue Message-ID: <393480994@web.de> Thank you for all replies. Just a quick update: I have carbol-gentiana-violet, of course, I just forgot to mention it. Please tell me how to decolorize the fuchsin without affecting the violet, that would help me very much in this moment. Thank you! I'll post my results and my method of course as soon as I get good results. _______________________________________________________________________ Jetzt neu! Sch?tzen Sie Ihren PC mit McAfee und WEB.DE. 30 Tage kostenlos testen. http://www.pc-sicherheit.web.de/startseite/?mc=022220 From mtighe <@t> trudeauinstitute.org Fri Nov 7 09:00:09 2008 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Fri Nov 7 08:56:29 2008 Subject: [Histonet] Liver Message-ID: <491410C3.26E4.00EE.0@trudeauinstitute.org> I am am having a difficult time getting immuno fluorescent staining in mouse liver. I am using markers that work side by side in lung tissue (F4/80, GR1). Does anyone have any suggestions for Immuno Fluorescent staining in mouse tissue or similar problems? Thanks!! From gu.lang <@t> gmx.at Fri Nov 7 09:40:54 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Nov 7 09:41:17 2008 Subject: AW: [Histonet] Advice In-Reply-To: References: Message-ID: <1D3FE6C26C4E43C6B37EB19F8A6AB3E2@dielangs.at> Our specimen in histo are from regular histo, cyto and morgue. We distinguish the incoming number with a letter at the beginning: Eg. C 00123/08 or O 000123/08 ... in the IT-System it is found as C0800123 and so on. For your specimen it could be M for mouse, R for rabbit, H for human .... Is that a useful hint? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Ian Montgomery Gesendet: Freitag, 07. November 2008 12:45 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Advice Currently, in my tissue log book I have a column headed, FIXATION NUMBER. Here I give every new piece of tissue I receive a sequential number, year and the next number. Now that my brief includes human anatomy I think that this simple term might not be enough. The tissue will still have the same number; it's just that I think a new column heading is needed. What I'm looking for is the term widely used in labs that would cover everything from pieces of research mammalian tissue to human material. Isn't it always the simplest things that really tax the brain? Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Nov 7 09:47:22 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Nov 7 09:47:43 2008 Subject: AW: [Histonet] CD4 on Ventana In-Reply-To: <2D0669F5E5EA4786A17AE07D652FFF51@pentium4> References: <30529BC228AC4005A8D771B85D58DDAE@IBLS.GLA.AC.UK><942D649BB02949728849D9DAA37C337F@Ford> <2D0669F5E5EA4786A17AE07D652FFF51@pentium4> Message-ID: CD4 4B12 Novocastra NCL-L-CD4-368 40 min, 37?C, Amplifier CC1 mild 1:20 (I not absolutly sure if I remember the titer right) Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Piero Nelva Gesendet: Freitag, 07. November 2008 10:15 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] CD4 on Ventana Dear Histonetters I'm having a lot of trouble getting CD4 to stain FFPE tissue using the Ventana Benchmark XT. Any advice about which antibody and protocol suggestions would be greatly appreciated. Piero Nelva Anatomical Pathology Monash Medical Centre Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmilne <@t> bccancer.bc.ca Fri Nov 7 10:20:50 2008 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Fri Nov 7 10:20:56 2008 Subject: [Histonet] RE:CD4 on Ventana In-Reply-To: References: Message-ID: <07979E76B0869D4E8C9FE4AA9FC0657804669616@srvex03.phsabc.ehcnet.ca> Hi Piero, what clone are you using for CD4? There seem to be 2 main clones, 1F6 or something like that and 4B12. The epitope recognized by 1F6 doesn't do well in formalin so 4B12 is the one most used. Having said that, I've found it's still not wonderful, I can get staining (I use a Ventana Discovery) but because the Ab is recommended to be used at such a high concentration, you do have to pick out the real staining against some background. I used it in this paper, our general protocol is in there too but I'm not sure how much the benchmark differs from the discovery.... http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.00034 09 Katy Milne Deeley Research Centre Message: 19 Date: Fri, 7 Nov 2008 20:15:11 +1100 From: "Piero Nelva" Subject: [Histonet] CD4 on Ventana To: Message-ID: <2D0669F5E5EA4786A17AE07D652FFF51@pentium4> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Dear Histonetters I'm having a lot of trouble getting CD4 to stain FFPE tissue using the Ventana Benchmark XT. Any advice about which antibody and protocol suggestions would be greatly appreciated. Piero Nelva Anatomical Pathology Monash Medical Centre Australia From Wanda.Smith <@t> HCAhealthcare.com Fri Nov 7 12:30:37 2008 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Fri Nov 7 12:31:21 2008 Subject: [Histonet] Is it Formalin-Without Smelling It? Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA9437324B@NADCWPMSGCMS03.hca.corpad.net> Happy Friday Everyone!!!!! Silly question: Is there an easy way to tell if a solution in a container is/is not formalin without smelling it????? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax From rcharles <@t> state.pa.us Fri Nov 7 12:42:56 2008 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Fri Nov 7 12:43:01 2008 Subject: [Histonet] RE: Is it Formalin-Without Smelling It? In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA9437324B@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <1B2F365C5F88A946B7D602E64ACD9CDD03E5765110@ENHBGMBX04.PA.LCL> We are using Shiffs reagent. When you put a drop of formalin in Shiffs reagent it turns purple. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Friday, November 07, 2008 1:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Is it Formalin-Without Smelling It? Happy Friday Everyone!!!!! Silly question: Is there an easy way to tell if a solution in a container is/is not formalin without smelling it????? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Nov 7 12:52:25 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Nov 7 12:52:25 2008 Subject: [Histonet] RE:CD4 on Ventana In-Reply-To: <07979E76B0869D4E8C9FE4AA9FC0657804669616@srvex03.phsabc.ehcnet.ca> Message-ID: True the 4b12 cd4 clone performs better in paraffin, still as you say, cd4 is just not the most robust ab in my hands either, one thing I have found (actually I need to credit Bryan Hewlett for this), is that you need to do endogenous h202 block after hier and you should use h202 in methanol, perhaps it is something about the methanol fixing after hier for ar that helps with cd4. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Milne, Katy Sent: Friday, November 07, 2008 9:21 AM To: histonet@lists.utsouthwestern.edu; pieronelva01@bigpond.com Subject: [Histonet] RE:CD4 on Ventana Hi Piero, what clone are you using for CD4? There seem to be 2 main clones, 1F6 or something like that and 4B12. The epitope recognized by 1F6 doesn't do well in formalin so 4B12 is the one most used. Having said that, I've found it's still not wonderful, I can get staining (I use a Ventana Discovery) but because the Ab is recommended to be used at such a high concentration, you do have to pick out the real staining against some background. I used it in this paper, our general protocol is in there too but I'm not sure how much the benchmark differs from the discovery.... http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.00034 09 Katy Milne Deeley Research Centre Message: 19 Date: Fri, 7 Nov 2008 20:15:11 +1100 From: "Piero Nelva" Subject: [Histonet] CD4 on Ventana To: Message-ID: <2D0669F5E5EA4786A17AE07D652FFF51@pentium4> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Dear Histonetters I'm having a lot of trouble getting CD4 to stain FFPE tissue using the Ventana Benchmark XT. Any advice about which antibody and protocol suggestions would be greatly appreciated. Piero Nelva Anatomical Pathology Monash Medical Centre Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Nov 7 12:54:57 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Nov 7 12:55:03 2008 Subject: [Histonet] Liver In-Reply-To: <491410C3.26E4.00EE.0@trudeauinstitute.org> Message-ID: Mike are you using formalin fixed paraffin embedded (ffpe) tissue for the IF staining? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Friday, November 07, 2008 8:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Liver I am am having a difficult time getting immuno fluorescent staining in mouse liver. I am using markers that work side by side in lung tissue (F4/80, GR1). Does anyone have any suggestions for Immuno Fluorescent staining in mouse tissue or similar problems? Thanks!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Nov 7 13:00:02 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Nov 7 13:00:05 2008 Subject: [Histonet] Mouse antibody to fibroblast without human crossreactivity? In-Reply-To: <450012a20811061236h6daa8b2bs7da53c0e7c146d21@mail.gmail.com> Message-ID: I do not think a marker like that exists, if it does I would like to know about it too. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suhyoung Jeong Sent: Thursday, November 06, 2008 1:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse antibody to fibroblast without human crossreactivity? Hello everyone, I am looking for a primary antibody that will recognize a protein in mouse fibroblast without crossreactivity to human. If you have any experience in this matter, please kindly let me know. Thank you in advance Suh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Fri Nov 7 13:11:59 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Nov 7 13:12:03 2008 Subject: [Histonet] schiff's Message-ID: Schiff's reagent is my nemesis. It's clear until it gets on anything outside of the bottle. Including the floor, clothing, and our fume hood. (Our post-doc had a little accident. Actually a rather large one, involving 1 L of Schiff's.) Emily On Fri, Nov 7, 2008 at 1:42 PM, Charles, Roger wrote: > We are using Shiffs reagent. When you put a drop of formalin in Shiffs > reagent it turns purple. > > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda > Sent: Friday, November 07, 2008 1:31 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Is it Formalin-Without Smelling It? > > Happy Friday Everyone!!!!! > Silly question: Is there an easy way to tell if a solution in a container > is/is not formalin without smelling it????? > Thanks, > Wanda > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- "You would know her for all the things she was...a woman who knew her way in and out of every new book without being singed, pinched, bumped or tickled by any line or chapter." John O'Hara, Appointment in Samarra From SwainFrancesL <@t> uams.edu Fri Nov 7 13:19:11 2008 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Fri Nov 7 13:19:31 2008 Subject: [Histonet] schiff's In-Reply-To: References: Message-ID: If you have a staining problem from the spill a weak solution of bleach usually clears up any Schiff's that is dropped on the bench, floor, hands, etc. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, November 07, 2008 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] schiff's Schiff's reagent is my nemesis. It's clear until it gets on anything outside of the bottle. Including the floor, clothing, and our fume hood. (Our post-doc had a little accident. Actually a rather large one, involving 1 L of Schiff's.) Emily On Fri, Nov 7, 2008 at 1:42 PM, Charles, Roger wrote: > We are using Shiffs reagent. When you put a drop of formalin in Shiffs > reagent it turns purple. > > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda > Sent: Friday, November 07, 2008 1:31 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Is it Formalin-Without Smelling It? > > Happy Friday Everyone!!!!! > Silly question: Is there an easy way to tell if a solution in a container > is/is not formalin without smelling it????? > Thanks, > Wanda > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- "You would know her for all the things she was...a woman who knew her way in and out of every new book without being singed, pinched, bumped or tickled by any line or chapter." John O'Hara, Appointment in Samarra _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From azdudley <@t> hotmail.com Fri Nov 7 13:21:52 2008 From: azdudley <@t> hotmail.com (anita dudley) Date: Fri Nov 7 13:21:56 2008 Subject: [Histonet] trichromes on ventana nexeES Message-ID: we are doing trichromes for our microbiology dept on their stool samples. is anyone doing this? we are having trouble with the controls that they purchase staining on the slide. just wondering if anyone else is doing this and what they have done to keep the spec on the slide. the pt slides seem to be fine. thanks a lot, everyone have a good weekend!!! anita dudley providence hosp mobile alabama _________________________________________________________________ See how Windows? connects the people, information, and fun that are part of your life http://clk.atdmt.com/MRT/go/119463819/direct/01/ From Wanda.Smith <@t> HCAhealthcare.com Fri Nov 7 13:34:43 2008 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Fri Nov 7 13:35:37 2008 Subject: [Histonet] RE: Is it Formalin-Without Smelling It? In-Reply-To: <1B2F365C5F88A946B7D602E64ACD9CDD03E5765110@ENHBGMBX04.PA.LCL> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA9437324B@NADCWPMSGCMS03.hca.corpad.net> <1B2F365C5F88A946B7D602E64ACD9CDD03E5765110@ENHBGMBX04.PA.LCL> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA9437333C@NADCWPMSGCMS03.hca.corpad.net> I knew there was a trick, I just couldn't remember what it was. Thanks everyone!!!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Friday, November 07, 2008 1:43 PM To: Smith Wanda; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Is it Formalin-Without Smelling It? We are using Shiffs reagent. When you put a drop of formalin in Shiffs reagent it turns purple. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Friday, November 07, 2008 1:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Is it Formalin-Without Smelling It? Happy Friday Everyone!!!!! Silly question: Is there an easy way to tell if a solution in a container is/is not formalin without smelling it????? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Fri Nov 7 13:39:52 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Nov 7 13:39:58 2008 Subject: [Histonet] 200 proof ethanol Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6583@nmdamailsvr.nmda.ad.nmsu.edu> I remember in the Olde Days (1971-76 or so) we got our ethanol in 50 gallon drums and it was my job to color the alcohol with something pink so it would be "recognized". Don't remember what the "pink" stuff was and I'm sure there was a reason for coloring it but I was young and didn't think I needed to know that, apparently. I did that, as I remember, after gathering eggs from the chicken house for the albumen and fighting the bees off for the wax... nah! - I'm not THAT old! I do remember round snap cassettes and L-shaped molds and sharpening knives on a glass plate.. maybe it's time for me to retire. Come to think of it - my ASCP certificate tells me that on March 7, 2009, I will have been practicing histology for FORTY years. Maybe I do remember the wax and chickens... And maybe I need to keep practicing! That's my Friday Fume for this L-O-N-G very interesting week in the world... adios. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From POWELL_SA <@t> Mercer.edu Fri Nov 7 13:53:56 2008 From: POWELL_SA <@t> Mercer.edu (powell_sa) Date: Fri Nov 7 13:49:21 2008 Subject: [Histonet] 200 proof ethanol Message-ID: <4916D31B@webmail.mercer.edu> Got you beat by 6 years Sara, and I remember all those fun things too, except I did not visit the bees. And the reason you colored the alcohol was to keep the interns and residents from having a party and using it all up. I also remember making hematoxylin and our ceiling had a huge purple circle right over the counter where we made it and "blew" it up. :) Those were the good ole days, well old anyway. Shirley >===== Original Message From "Breeden, Sara" ===== >I remember in the Olde Days (1971-76 or so) we got our ethanol in 50 >gallon drums and it was my job to color the alcohol with something pink >so it would be "recognized". Don't remember what the "pink" stuff was >and I'm sure there was a reason for coloring it but I was young and >didn't think I needed to know that, apparently. I did that, as I >remember, after gathering eggs from the chicken house for the albumen >and fighting the bees off for the wax... nah! - I'm not THAT old! I do >remember round snap cassettes and L-shaped molds and sharpening knives >on a glass plate.. maybe it's time for me to retire. Come to think of >it - my ASCP certificate tells me that on March 7, 2009, I will have >been practicing histology for FORTY years. Maybe I do remember the wax >and chickens... And maybe I need to keep practicing! That's my Friday >Fume for this L-O-N-G very interesting week in the world... adios. > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 4700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Fri Nov 7 13:51:28 2008 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri Nov 7 13:52:45 2008 Subject: [Histonet] Re: 200Proof Message-ID: <11D9615B89C10747B1C985966A63D7CA286ECA88F5@KCL-MAIL04.kclad.ds.kcl.ac.uk> Just to try to help to make clear to those of us who get confused when people talk about "proof" and % alcohol, I would recommend reading : http://en.wikipedia.org/wiki/Proof_spirit Proof is all about NOT getting a BANG from gunpowder ;-) In UK Histopath/Histology labs, we traditionally use 64OP and/or 74OP Spirit, to confuse the post a little: this is NOT pure ethanol but Industrial Methylated Spirit (IMS). OP= Over-Proof, an antiquated English Customs and Excise term but, a nice and precise term/measure. If you want to test it with Gunpowder to make sure.....you run the risk of being arrested as a terrorist. IMS does not incur taxation/regulation , in the way that ethanol does, because Methanol is added to the Ethanol base. ( under increasing EU regulations I may be wrong,now) Anyone who uses Ethanol for tissue processing/de-rehydrating is incurring, in many Countries, imho, extra irrelevant costs. Sure, many Histonetters do not have a choice. Carl From ryaskovich <@t> dir.nidcr.nih.gov Fri Nov 7 13:53:32 2008 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Fri Nov 7 13:53:44 2008 Subject: [Histonet] 200 proof ethanol In-Reply-To: <4916D31B@webmail.mercer.edu> References: <4916D31B@webmail.mercer.edu> Message-ID: I remember working for the Maryland Department of Agriculture and our formalin was pink. I think it was actually Embalming Fluid! Ruth Yaskovich N.I.H. National Institute of Dental and Crainiofacial Research Neurobiology and Pain Branch -----Original Message----- From: powell_sa [mailto:POWELL_SA@Mercer.edu] Sent: Friday, November 07, 2008 2:54 PM To: Breeden, Sara; histonet Subject: RE: [Histonet] 200 proof ethanol Got you beat by 6 years Sara, and I remember all those fun things too, except I did not visit the bees. And the reason you colored the alcohol was to keep the interns and residents from having a party and using it all up. I also remember making hematoxylin and our ceiling had a huge purple circle right over the counter where we made it and "blew" it up. :) Those were the good ole days, well old anyway. Shirley >===== Original Message From "Breeden, Sara" ===== >I remember in the Olde Days (1971-76 or so) we got our ethanol in 50 >gallon drums and it was my job to color the alcohol with something pink >so it would be "recognized". Don't remember what the "pink" stuff was >and I'm sure there was a reason for coloring it but I was young and >didn't think I needed to know that, apparently. I did that, as I >remember, after gathering eggs from the chicken house for the albumen >and fighting the bees off for the wax... nah! - I'm not THAT old! I do >remember round snap cassettes and L-shaped molds and sharpening knives >on a glass plate.. maybe it's time for me to retire. Come to think of >it - my ASCP certificate tells me that on March 7, 2009, I will have >been practicing histology for FORTY years. Maybe I do remember the wax >and chickens... And maybe I need to keep practicing! That's my Friday >Fume for this L-O-N-G very interesting week in the world... adios. > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 4700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Nov 7 13:53:23 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Nov 7 13:54:07 2008 Subject: [Histonet] 200 proof ethanol In-Reply-To: <4916D31B@webmail.mercer.edu> References: <4916D31B@webmail.mercer.edu> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C4AE@LTA3VS011.ees.hhs.gov> Many, many years ago I worked with a pathologist that told of parties with the waste (eosin-tinted) alcohols.......and he discovered that when he sweated he sweated pinkish-red! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of powell_sa Sent: Friday, November 07, 2008 2:54 PM To: Breeden, Sara; histonet Subject: RE: [Histonet] 200 proof ethanol Got you beat by 6 years Sara, and I remember all those fun things too, except I did not visit the bees. And the reason you colored the alcohol was to keep the interns and residents from having a party and using it all up. I also remember making hematoxylin and our ceiling had a huge purple circle right over the counter where we made it and "blew" it up. :) Those were the good ole days, well old anyway. Shirley >===== Original Message From "Breeden, Sara" >===== I remember in the Olde Days (1971-76 or so) we got our ethanol in >50 gallon drums and it was my job to color the alcohol with something >pink so it would be "recognized". Don't remember what the "pink" stuff >was and I'm sure there was a reason for coloring it but I was young and >didn't think I needed to know that, apparently. I did that, as I >remember, after gathering eggs from the chicken house for the albumen >and fighting the bees off for the wax... nah! - I'm not THAT old! I do >remember round snap cassettes and L-shaped molds and sharpening knives >on a glass plate.. maybe it's time for me to retire. Come to think of >it - my ASCP certificate tells me that on March 7, 2009, I will have >been practicing histology for FORTY years. Maybe I do remember the wax >and chickens... And maybe I need to keep practicing! That's my Friday >Fume for this L-O-N-G very interesting week in the world... adios. > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 4700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Fri Nov 7 14:21:59 2008 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Fri Nov 7 14:22:03 2008 Subject: [Histonet] RE: Is it Formalin-Without Smelling It? In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA9437333C@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA9437324B@NADCWPMSGCMS03.hca.corpad.net> <1B2F365C5F88A946B7D602E64ACD9CDD03E5765110@ENHBGMBX04.PA.LCL> <9E2D36CE2D7CBA4A94D9B22E8328A3BA9437333C@NADCWPMSGCMS03.hca.corpad.net> Message-ID: My favorite method is to have the container LABELED. Jerry Ricks Research Scientist University of Washington Department of Pathology > From: Wanda.Smith@HCAhealthcare.com > To: rcharles@state.pa.us; histonet@lists.utsouthwestern.edu > Date: Fri, 7 Nov 2008 13:34:43 -0600 > CC: > Subject: [Histonet] RE: Is it Formalin-Without Smelling It? > > I knew there was a trick, I just couldn't remember what it was. > Thanks everyone!!!!! > Wanda > > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger > Sent: Friday, November 07, 2008 1:43 PM > To: Smith Wanda; Histonet (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] RE: Is it Formalin-Without Smelling It? > > We are using Shiffs reagent. When you put a drop of formalin in Shiffs reagent it turns purple. > > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda > Sent: Friday, November 07, 2008 1:31 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Is it Formalin-Without Smelling It? > > Happy Friday Everyone!!!!! > Silly question: Is there an easy way to tell if a solution in a container is/is not formalin without smelling it????? > Thanks, > Wanda > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows Live Hotmail now works up to 70% faster. http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_faster_112008 From alexandra.meinl <@t> gmail.com Fri Nov 7 14:34:48 2008 From: alexandra.meinl <@t> gmail.com (Alexandra Meinl) Date: Fri Nov 7 14:34:52 2008 Subject: [Histonet] bone histology - preventing artifacts Message-ID: Hello Histonetters, When I compare my MMA bone histology with with paraffin bone sections I always notice the same artifact in the paraffin sections: The bone lining cells seem to fall off the surface, I also noticed osteoclasts here and there that were stripped off. Bone morphology is good (I used EDTA for decal). I suspect that maybe the dehydration was too fast for bone (I used the same protocol as for normal tissue specimens). What are your experiences with decalcified bone sections? Do you use a different protocol for your VIP? Or are these artifacts inevitable? Thanks, Alexandra From histotechkb <@t> gmail.com Fri Nov 7 14:38:07 2008 From: histotechkb <@t> gmail.com (Kristen Yaros) Date: Fri Nov 7 14:38:12 2008 Subject: [Histonet] Advice In-Reply-To: <1D3FE6C26C4E43C6B37EB19F8A6AB3E2@dielangs.at> References: <1D3FE6C26C4E43C6B37EB19F8A6AB3E2@dielangs.at> Message-ID: <667c97ab0811071238p71f28874leae122ac09a3533f@mail.gmail.com> Might need to expand that type of system to Ms, Rb, Rt, etc.. Wouldn't want to look back & wonder if it was a Rabbit or Rat. But yes, it gives you a starting point! On 11/7/08, Gudrun Lang wrote: > > Our specimen in histo are from regular histo, cyto and morgue. We > distinguish the incoming number with a letter at the beginning: > Eg. C 00123/08 or O 000123/08 ... in the IT-System it is found as C0800123 > and so on. > > For your specimen it could be M for mouse, R for rabbit, H for human .... > Is that a useful hint? > Gudrun > > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Ian > Montgomery > Gesendet: Freitag, 07. November 2008 12:45 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Advice > > Currently, in my tissue log book I have a column headed, > FIXATION NUMBER. Here I give every new piece of tissue I receive a > sequential number, year and the next number. Now that my brief includes > human anatomy I think that this simple term might not be enough. The tissue > will still have the same number; it's just that I think a new column > heading > is needed. What I'm looking for is the term widely used in labs that would > cover everything from pieces of research mammalian tissue to human > material. > > Isn't it always the simplest things that really tax the brain? > > > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Kristen Yaros, HT (ASCP)CM Histotechnology Society of Delaware Correspondence Secretary histotechkb@gmail.com From JMaslanka <@t> stpetes.org Fri Nov 7 14:42:32 2008 From: JMaslanka <@t> stpetes.org (JMaslanka@stpetes.org) Date: Fri Nov 7 14:44:26 2008 Subject: [Histonet] Job Opening Message-ID: Good techs are hard to find. St Peters Hospital in Helena Montana has an opening for a histotech. Great place to work, even more place to live. If interested check out www.stpetes.org Joe Maslanka Cyto/Histo Coord. St Peter's Laboratory " Not everything that can be counted counts..... Not everything that counts can be counted." Albert Einstein From Wanda.Smith <@t> HCAhealthcare.com Fri Nov 7 14:53:19 2008 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Fri Nov 7 14:53:26 2008 Subject: [Histonet] RE: Is it Formalin-Without Smelling It? In-Reply-To: References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA9437324B@NADCWPMSGCMS03.hca.corpad.net> <1B2F365C5F88A946B7D602E64ACD9CDD03E5765110@ENHBGMBX04.PA.LCL> <9E2D36CE2D7CBA4A94D9B22E8328A3BA9437333C@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA94373441@NADCWPMSGCMS03.hca.corpad.net> What a concept?!?!? WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Friday, November 07, 2008 3:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Is it Formalin-Without Smelling It? My favorite method is to have the container LABELED. Jerry Ricks Research Scientist University of Washington Department of Pathology > From: Wanda.Smith@HCAhealthcare.com > To: rcharles@state.pa.us; histonet@lists.utsouthwestern.edu > Date: Fri, 7 Nov 2008 13:34:43 -0600 > CC: > Subject: [Histonet] RE: Is it Formalin-Without Smelling It? > > I knew there was a trick, I just couldn't remember what it was. > Thanks everyone!!!!! > Wanda > > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Charles, Roger > Sent: Friday, November 07, 2008 1:43 PM > To: Smith Wanda; Histonet (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] RE: Is it Formalin-Without Smelling It? > > We are using Shiffs reagent. When you put a drop of formalin in Shiffs reagent it turns purple. > > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith > Wanda > Sent: Friday, November 07, 2008 1:31 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Is it Formalin-Without Smelling It? > > Happy Friday Everyone!!!!! > Silly question: Is there an easy way to tell if a solution in a container is/is not formalin without smelling it????? > Thanks, > Wanda > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows Live Hotmail now works up to 70% faster. http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_faster_112008_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGrobe2555 <@t> aol.com Fri Nov 7 14:59:17 2008 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Fri Nov 7 14:59:48 2008 Subject: [Histonet] RE: Is it Formalin-Without Smelling It? Message-ID: In our lab, if it isn't labeled, it gets thrown out. But we work in research and not in a clinical setting.... Albert Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana Foundation **************AOL Search: Your one stop for directions, recipes and all other Holiday needs. Search Now. (http://pr.atwola.com/promoclk/100000075x1212792382x1200798498/aol?redir=http://searchblog.aol.com/2008/11/04/happy-holidays-from -aol-search/?ncid=emlcntussear00000001) From Jackie.O'Connor <@t> abbott.com Fri Nov 7 15:11:15 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Nov 7 15:11:36 2008 Subject: [Histonet] RE: Is it Formalin-Without Smelling It? In-Reply-To: Message-ID: Years ago, we added methyl violet to our formalin which enabled us to quickly identify the solution in or out of a container. It also serves as a pH indicator, turns yellow when acidic. That came in handy for long term fixation of large bloody specimens that might have required a formalin change to ensure good fixation. JR R Sent by: histonet-bounces@lists.utsouthwestern.edu 11/07/2008 02:21 PM To cc Subject [Histonet] RE: Is it Formalin-Without Smelling It? My favorite method is to have the container LABELED. Jerry Ricks Research Scientist University of Washington Department of Pathology > From: Wanda.Smith@HCAhealthcare.com > To: rcharles@state.pa.us; histonet@lists.utsouthwestern.edu > Date: Fri, 7 Nov 2008 13:34:43 -0600 > CC: > Subject: [Histonet] RE: Is it Formalin-Without Smelling It? > > I knew there was a trick, I just couldn't remember what it was. > Thanks everyone!!!!! > Wanda > > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger > Sent: Friday, November 07, 2008 1:43 PM > To: Smith Wanda; Histonet (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] RE: Is it Formalin-Without Smelling It? > > We are using Shiffs reagent. When you put a drop of formalin in Shiffs reagent it turns purple. > > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda > Sent: Friday, November 07, 2008 1:31 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Is it Formalin-Without Smelling It? > > Happy Friday Everyone!!!!! > Silly question: Is there an easy way to tell if a solution in a container is/is not formalin without smelling it????? > Thanks, > Wanda > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows Live Hotmail now works up to 70% faster. http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_faster_112008_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Fri Nov 7 15:19:58 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Nov 7 15:20:24 2008 Subject: [Histonet] All is revealed. In-Reply-To: References: Message-ID: Ian, I believe that you were thinking along the correct lines... "unscibe" is actually the root origin of the modern day word "unsubscribe" I believe it comes from the early Pictish language spoken in northern & central Scotland in the middle ages, meaning: "to doodle without a pict (or Pict)". Your patois just hasn't made it to the 21st centaury as yet. Ford -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Friday, November 07, 2008 5:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] All is revealed. Ford, Many thanks and me thinking it was simply, unscibe. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail <@t> bellsouth.net Fri Nov 7 17:05:52 2008 From: renafail <@t> bellsouth.net (renafail@bellsouth.net) Date: Fri Nov 7 17:05:58 2008 Subject: [Histonet] schiff's In-Reply-To: References: Message-ID: <110720082305.23206.4914C9D0000499E200005AA622230650029B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net> A Liter!!! It took most of a day to clean 40 ml dropped in a dishwasher to which the tech added extra multiterge, which caused the dishwasher to overflow. A colorful event. My sympathy Rena Fail -------------- Original message from "Swain, Frances L" : -------------- > If you have a staining problem from the spill a weak solution of bleach > usually clears up any Schiff's that is dropped on the bench, floor, hands, > etc. > > Frances L. Swain HT(ASCP) A. A. S. > Special Procedures Technician > Department of Orthopaedic Surgery > Center for Orthopaedic Research > Barton Research Building 2R28 > 4301 West Markham Street > Little Rock AR 72205 > (501) 686-8739 PHONE > (501) 686-8987 FAX > swainfrancesl@uams.edu email > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Friday, November 07, 2008 1:12 PM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] schiff's > > Schiff's reagent is my nemesis. It's clear until it gets on anything > outside of the bottle. > Including the floor, clothing, and our fume hood. > (Our post-doc had a little accident. Actually a rather large one, involving > 1 L of Schiff's.) > > Emily > > On Fri, Nov 7, 2008 at 1:42 PM, Charles, Roger wrote: > > > We are using Shiffs reagent. When you put a drop of formalin in Shiffs > > reagent it turns purple. > > > > Roger Charles > > Microbiologist > > Pennsylvania Veterinary Laboratory > > 2305 N Cameron St > > Harrisburg, PA 17110 > > 717-787-8808 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda > > Sent: Friday, November 07, 2008 1:31 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Is it Formalin-Without Smelling It? > > > > Happy Friday Everyone!!!!! > > Silly question: Is there an easy way to tell if a solution in a container > > is/is not formalin without smelling it????? > > Thanks, > > Wanda > > > > WANDA G. SMITH, HTL(ASCP)HT > > Pathology Supervisor > > TRIDENT MEDICAL CENTER > > 9330 Medical Plaza Drive > > Charleston, SC 29406 > > 843-847-4586 > > 843-847-4296 fax > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > "You would know her for all the things she was...a woman who knew her way in > and out of every new book without being singed, pinched, bumped or tickled > by any line or chapter." > John O'Hara, Appointment in Samarra > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: This e-mail message, including any attachments, is for > the sole use of the intended recipient(s) and may contain confidential and > privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From saby_joseph_a <@t> yahoo.com Fri Nov 7 18:35:33 2008 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Fri Nov 7 18:35:38 2008 Subject: [Histonet] schiff's References: <110720082305.23206.4914C9D0000499E200005AA622230650029B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net> Message-ID: <97562.45000.qm@web33801.mail.mud.yahoo.com> I had?a little problem once with Schiffs. I was making up a new batch (I believe about 2 liters) and had just finished boiling the water.? I was in a hurry (often the culprit in disasterous lab events) and forgat that impure solvents have a lower boiuling point.? When?I added the basic fuchsin, it erupted like a volcano! Now, to get the full picture, you must envision a newly remodeled lab.? New tile floor.? Newly painted cupboards.? Fresh paint on the wall.? And, of course, the lab director a planning walk-through show-off tour of the new facility. Bucket and mop to try to remove most of the excess.? Gallons of acid alcohol.? And, yes, 10% bleach solution for the fresh paint and the cabinets.? It took all day, but I got it looking good as new. The next week, someone broke a container of eosin on the floor.? My boss forever blamed me for the orange-pink arising every time they mopped the floor... ________________________________ From: "renafail@bellsouth.net" To: "Swain, Frances L" ; Emily Sours ; histonet@lists.utsouthwestern.edu Sent: Friday, November 7, 2008 6:05:52 PM Subject: RE: [Histonet] schiff's A Liter!!! It took most of a day to clean 40 ml dropped in a dishwasher? to which the tech added? extra multiterge, which caused the dishwasher to overflow. A colorful event. My sympathy Rena Fail -------------- Original message from "Swain, Frances L" : -------------- > If you have a staining problem from the spill a weak solution of bleach > usually clears up any Schiff's that is dropped on the bench, floor, hands, > etc. > > Frances L. Swain HT(ASCP) A. A. S. > Special Procedures Technician > Department of Orthopaedic Surgery > Center for Orthopaedic Research > Barton Research Building 2R28 > 4301 West Markham Street > Little Rock AR 72205 > (501) 686-8739 PHONE > (501) 686-8987 FAX > swainfrancesl@uams.edu email > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Friday, November 07, 2008 1:12 PM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] schiff's > > Schiff's reagent is my nemesis.? It's clear until it gets on anything > outside of the bottle. > Including the floor, clothing, and our fume hood. > (Our post-doc had a little accident.? Actually a rather large one, involving > 1 L of Schiff's.) > > Emily > > On Fri, Nov 7, 2008 at 1:42 PM, Charles, Roger? wrote: > > > We are using Shiffs reagent.? When you put a drop of formalin in Shiffs > > reagent it turns purple. > > > > Roger Charles > > Microbiologist > > Pennsylvania Veterinary Laboratory > > 2305 N Cameron St > > Harrisburg, PA 17110 > > 717-787-8808 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda > > Sent: Friday, November 07, 2008 1:31 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Is it Formalin-Without Smelling It? > > > > Happy Friday Everyone!!!!! > > Silly question:? Is there an easy way to tell if a solution in a container > > is/is not formalin without smelling it????? > > Thanks, > > Wanda > > > > WANDA G. SMITH, HTL(ASCP)HT > > Pathology Supervisor > > TRIDENT MEDICAL CENTER > > 9330 Medical Plaza Drive > > Charleston, SC? 29406 > > 843-847-4586 > > 843-847-4296 fax > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > "You would know her for all the things she was...a woman who knew her way in > and out of every new book without being singed, pinched, bumped or tickled > by any line or chapter." > John O'Hara, Appointment in Samarra > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: This e-mail message, including any attachments, is for > the sole use of the intended recipient(s) and may contain confidential and > privileged information.? Any unauthorized review, use, disclosure or > distribution is prohibited.? If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From angelafogg <@t> aol.com Fri Nov 7 19:16:48 2008 From: angelafogg <@t> aol.com (angelafogg@aol.com) Date: Fri Nov 7 19:16:59 2008 Subject: [Histonet] Trichrome Question Message-ID: <8CB0F56CEF9B544-10E4-1221@WEBMAIL-MY18.sysops.aol.com> Performed a trichrome stain on a piece of colon which had been in formalin for? weeks.? Muscle stained blue instead of red.?What happened?? Does prolonged fixation react this way? Hope someone can shed some light on this. Regards, Angela From drmoses111 <@t> comcast.net Fri Nov 7 21:24:16 2008 From: drmoses111 <@t> comcast.net (drmoses111@comcast.net) Date: Fri Nov 7 21:24:22 2008 Subject: [Histonet] Re: Histonet Digest, Vol 60, Issue 15 Message-ID: <110820080324.7944.49150660000A577100001F082200735834CECECE9C0A9C01039D0B@comcast.net> Position open in our histology department. Good skills required, registered HT/HTL preferred. Send resume drmoses111@comcast.net -------------- Original message -------------- From: histonet-request@lists.utsouthwestern.edu > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > From annigyg <@t> gmail.com Fri Nov 7 22:13:38 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Fri Nov 7 22:13:44 2008 Subject: [Histonet] schiff's In-Reply-To: References: Message-ID: quick question: does the formalin turn deep magenta or purple when you add the schiffs i have had a problem with paths saying they dislike the 'blueish' hues (purple) of the schiffs in the pas done on the Artisan we now do them all manually with our in-house schiffs (deep pink/magenta) no HX overstaining anyone out there having similar problems? AbuDhabiAnnie 2008/11/7 Emily Sours > Schiff's reagent is my nemesis. It's clear until it gets on anything > outside of the bottle. > Including the floor, clothing, and our fume hood. > (Our post-doc had a little accident. Actually a rather large one, > involving > 1 L of Schiff's.) > > Emily > > On Fri, Nov 7, 2008 at 1:42 PM, Charles, Roger > wrote: > > > We are using Shiffs reagent. When you put a drop of formalin in Shiffs > > reagent it turns purple. > > > > Roger Charles > > Microbiologist > > Pennsylvania Veterinary Laboratory > > 2305 N Cameron St > > Harrisburg, PA 17110 > > 717-787-8808 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda > > Sent: Friday, November 07, 2008 1:31 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Is it Formalin-Without Smelling It? > > > > Happy Friday Everyone!!!!! > > Silly question: Is there an easy way to tell if a solution in a > container > > is/is not formalin without smelling it????? > > Thanks, > > Wanda > > > > WANDA G. SMITH, HTL(ASCP)HT > > Pathology Supervisor > > TRIDENT MEDICAL CENTER > > 9330 Medical Plaza Drive > > Charleston, SC 29406 > > 843-847-4586 > > 843-847-4296 fax > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > "You would know her for all the things she was...a woman who knew her way > in > and out of every new book without being singed, pinched, bumped or tickled > by any line or chapter." > John O'Hara, Appointment in Samarra > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From sccrshlly <@t> yahoo.com Fri Nov 7 22:26:12 2008 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Fri Nov 7 22:26:17 2008 Subject: [Histonet] Re: Advice Message-ID: <549276.38254.qm@web90303.mail.mud.yahoo.com> If you are looking for the title/heading for the column, I would suggest "Tissue Type Submitted".? Hope this helps. ? Michelle From jkiernan <@t> uwo.ca Sat Nov 8 01:09:46 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Nov 8 01:09:51 2008 Subject: [Histonet] Trichrome Question Message-ID: Which trichrome method? What kind of section (frozen, paraffin, plastic?) Any post-fixation in something other than plain formalin before staining? Provide the technical details, and you will get lots of advice! Weeks in formaldehyde is not "prolonged" fixation unless the weeks are numerous enough to make years. As a generality, trichrome methods do not work very well after fixation in liquids that have formaldehyde as the only active ingredient. Postfixation of the sections can compensate. Bouin is frequently used. Saturated aqueous picric acid us just as good. There are published reports that iodine and even citrate buffer will improve trichrome staining of paraffin sections of formaldehyde-fixed tissue. "Trichrome" has been applied to several staining techniques that use two or more dyes. By convention, since about 1920, trichrome methods have been those using phosphomolybdic or phosphotungstic acid (or both) to enable the staining of collagen and cytoplasm by anionic dyes with sharply contrasting colours: blue or green for collagen, and red for cytoplasm (including smooth & striated muscle). A third anionic dye, typically yellow or orange, may be added to stain red blood cells. Instructions for trichrome methods can be found in all textbooks of microtechnique and histotechnology. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: angelafogg@aol.com Date: Friday, November 7, 2008 20:17 Subject: [Histonet] Trichrome Question To: histonet@lists.utsouthwestern.edu > > Performed a trichrome stain on a piece of colon which had been > in formalin for? weeks.? Muscle stained blue instead of > red.?What happened?? Does prolonged fixation react this way? > Hope someone can shed some light on this. > Regards, > Angela > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Nov 8 09:48:06 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Nov 8 09:48:16 2008 Subject: [Histonet] Is it Formalin-Without Smelling It? In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA9437324B@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <678639.89326.qm@web65713.mail.ac4.yahoo.com> Yes, add Shiff reagent and if it turns violet-purple, it contains an aldehyde, most likely. formalin in your case. Ren? J. --- On Fri, 11/7/08, Smith Wanda wrote: From: Smith Wanda Subject: [Histonet] Is it Formalin-Without Smelling It? To: "histonet@lists.utsouthwestern.edu" Date: Friday, November 7, 2008, 1:30 PM Happy Friday Everyone!!!!! Silly question: Is there an easy way to tell if a solution in a container is/is not formalin without smelling it????? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From epearlstein <@t> hotmail.com Sat Nov 8 13:52:02 2008 From: epearlstein <@t> hotmail.com (Ellen Pearlstein) Date: Sat Nov 8 13:52:07 2008 Subject: FW: [Histonet] REMOVE FROM LIST In-Reply-To: References: Message-ID: REMOVE ME FROM THE LIST PLEASE> From: epearlstein@hotmail.com> To: histonet@lists.utsouthwestern.edu> Date: Thu, 6 Nov 2008 13:35:11 -0800> Subject: [Histonet] REMOVE FROM LIST> > > please remove me from the list.> > _________________________________________________________________> Color coding for safety: Windows Live Hotmail alerts you to suspicious email.> http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_safety_112008_______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows Live Hotmail now works up to 70% faster. http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_faster_112008 From drmoses111 <@t> comcast.net Sat Nov 8 18:29:26 2008 From: drmoses111 <@t> comcast.net (drmoses111@comcast.net) Date: Sat Nov 8 18:29:30 2008 Subject: [Histonet] Histology job opening in South Jersey Message-ID: <110920080029.15575.49162EE600001AD800003CD72213575333CECECE9C0A9C01039D0B@comcast.net> We have a position open in our Histology department. HT/HTL preferred , good skills required. Send resume : drmoses111@comcast.net From andreaharris24241997 <@t> yahoo.com Sat Nov 8 21:42:44 2008 From: andreaharris24241997 <@t> yahoo.com (Andrea Harris) Date: Sat Nov 8 21:42:48 2008 Subject: [Histonet] REmove me Message-ID: <420378.9742.qm@web34401.mail.mud.yahoo.com> From Shirley_PHUA <@t> hsa.gov.sg Sun Nov 9 14:03:55 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Sun Nov 9 14:04:11 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 10/11/2008 to 11/11/2008. 10 November 2008 9am - 5pm: New Performance Mgmt System (Train-the-Trainer) (HSA L2-4) 3pm - 5pm: Meeting (HSA L1-2) 5pm - 6pm: ASG Day Briefing (HSA Auditorium) 11 November 2008 9am - 5pm: New Performance Mgmt System (Train-the-Trainer) (HSA L2-4) 2pm - 5pm: BATA Training (Former CAS Resource Ctr) From AnthonyH <@t> chw.edu.au Sun Nov 9 15:52:40 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Nov 9 15:52:54 2008 Subject: [Histonet] Fatty tissue In-Reply-To: <719963.36031.qm@web65702.mail.ac4.yahoo.com> Message-ID: Yes, "anecdotal" but consistent Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Saturday, 8 November 2008 12:56 AM To: Tony Henwood; Webb, Dorothy L; histonet@lists.utsouthwestern.edu; Luck, Greg D. Subject: RE: [Histonet] Fatty tissue The MW irradiation will NOT accelerate formalin cross linking; essentially MW irradiation will have a "cooking" effect on tissue, that is why the "6-48" rule was issued by ASCO-CAP After MW irradiation the tissues that go into dehydration will be only partially fixed with formalin and will end being fixed by the alcohols in a "2 fixation" process with unknown consequences for the procedures that follow.The anecdotal "successful" use of MW for tissue fixation are just that, anecdotal, Ren? J. --- On Thu, 11/6/08, Luck, Greg D. wrote: From: Luck, Greg D. Subject: RE: [Histonet] Fatty tissue To: "Tony Henwood" , "Webb, Dorothy L" , histonet@lists.utsouthwestern.edu Date: Thursday, November 6, 2008, 7:14 PM Hello, Are people microwave fixing breast tissues? Just wondering as/does this fly in the face of the "6-48" hour fixation requirement for any breast tissue that is to have Her2-neu testing? Thanks, Greg ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Nov 9 16:00:09 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Nov 9 16:00:17 2008 Subject: [Histonet] Is it Formalin-Without Smelling It? In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA9437324B@NADCWPMSGCMS03.hca.corpad.net> Message-ID: An easily and rapidly applied technique can establish the presence or absence of formalin without placing the investigating staff in harm's way: Place a few drops of reticulin diamine silver solution in a beaker and add small drops of the test solution. If the test solution is formalin, the reticulin solution will turn black. A similar effect can be produced by adding the test solution to Schiff's reagent. In this case, adding drops of formalin will turn the combination a deep magenta colour. The addition of a test solution of saline (the most frequently encountered alternative) will produce no colour change to Schiff's solution but will turn the silver diamine solution white. Because all laboratories will have both reagents already prepared on their shelves, the test may be done in a matter of seconds (Grehan & McDermont (2001) J Clin Pathol 54:734-735). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith Wanda Sent: Saturday, 8 November 2008 5:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Is it Formalin-Without Smelling It? Happy Friday Everyone!!!!! Silly question: Is there an easy way to tell if a solution in a container is/is not formalin without smelling it????? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From anh2006 <@t> med.cornell.edu Sun Nov 9 22:50:36 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Sun Nov 9 22:50:50 2008 Subject: [Histonet] Testes vascular markers Message-ID: What are the best markers to stain testicular endothelium? Thanks, Andrea -- From jkiernan <@t> uwo.ca Mon Nov 10 01:05:37 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Nov 10 01:05:41 2008 Subject: [Histonet] Trichrome Question In-Reply-To: References: Message-ID: Dear Angela Fogg,
 
It is difficult to Gomori' published  20: 662-664 (1950)? Kiernan
Anatomy, UWO
Lon =
----- Original Message -----Date: Saturday, November 8 10:03
Subject: Re: [Histonet] Trichrome Questi on
To: jkiernan@uwo.ca

>
&g
> 6.00.6000.16735" name=GENERATOR> From Susan.Walzer <@t> HCAHealthcare.com Mon Nov 10 02:16:20 2008 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Mon Nov 10 02:16:25 2008 Subject: [Histonet] RE: Is it Formalin-Without Smelling It? In-Reply-To: References: Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2A9003D76D@FWDCWPMSGCMS09.hca.corpad.net> You need to send the bottle back to where it originated. They are the ones who are responsible for correctly labeling hazardous solutions. From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of AGrobe2555@aol.com Sent: Friday, November 07, 2008 3:59 PM To: Smith Wanda; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Is it Formalin-Without Smelling It? In our lab, if it isn't labeled, it gets thrown out. But we work in research and not in a clinical setting.... Albert Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana Foundation **************AOL Search: Your one stop for directions, recipes and all other Holiday needs. Search Now. (http://pr.atwola.com/promoclk/100000075x1212792382x1200798498/aol?redir=http://searchblog.aol.com/2008/11/04/happy-holidays-from -aol-search/?ncid=emlcntussear00000001) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Imcgonnell <@t> RVC.AC.UK Mon Nov 10 10:13:48 2008 From: Imcgonnell <@t> RVC.AC.UK (McGonnell, Imelda Mary) Date: Mon Nov 10 10:14:00 2008 Subject: [Histonet] Wanted: a user manual for the reichert jung frigocut 2800 E cryostat Message-ID: <193A454735ABFE438D08FA139B54B005058A9DBB@cmw2kex01.rvc.ac.uk> Hi, we are trying to get hold of a manual for the Reichert Jung frigocut 2800 E cryostat. Does anyone out there have one? If anyone does, we would be really grateful if you would be willing to send it out to us and we will photocopy and send back. Many thanks Imelda Dr Imelda McGonnell Lecturer Dept. Veterinary Basic Sciences Royal Veterinary College Royal College St London NW1 0TU Phone : Extn. 5453 Direct dial 020 7468 1223 From lcrosby <@t> echelon-inc.com Mon Nov 10 10:15:23 2008 From: lcrosby <@t> echelon-inc.com (Lee Crosby) Date: Mon Nov 10 10:18:56 2008 Subject: [Histonet] CELL CULTURE LINE ON SLIDES In-Reply-To: <24A4826E8EF0964D86BC5317306F58A52BA2A07DE2@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A52BA2A07DE2@mmc-mail.ad.mhsil.com> Message-ID: Jim, I routinely stain cells with fluorescent antibodies. I fix them with 4% Paraformaldehyde in PBS, or the easier way, which I use now, is in neutral buffered formalin, which is readily purchased and does not require the labor intensive steps of the former. Good luck. Lee Crosby Echelon Biosciences Inc. 801-588-0455 ext 329 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, November 03, 2008 12:58 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] CELL CULTURE LINE ON SLIDES We were asked to do immunostains on slides where a particular cell line was growing. Can anyone tell me the best way to fix these slides before performing immunostains? We used to use acetone on cytospins but can't recall if there is a better way. thanks Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy.jim@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From september.amspacher <@t> bassett.org Mon Nov 10 10:36:17 2008 From: september.amspacher <@t> bassett.org (Amspacher, September) Date: Mon Nov 10 10:36:22 2008 Subject: [Histonet] Looking for Lynn Ingram Message-ID: <9485D6C8CD957E488B81B56B0EBB28A44F4498@ex3.bassett.org> Hello all, I recently read a 2 part article in Advance magazine and am looking to speak with the author, I was hoping that someone on histonet might be able to help get in contact with her. Lynn Ingram MS, CLS(NCA) who wrote an article on "We used to love our jobs" Thanks for the help. September Amspacher HT(ASCP) Sr. Tech- Histologist Histology Department Bassett Healthcare, Cooperstown New York (607)547-6727 From Jackie.O'Connor <@t> abbott.com Mon Nov 10 10:59:11 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Nov 10 10:59:28 2008 Subject: [Histonet] Is there a Richard Allen Vendor in Europe? In-Reply-To: Message-ID: I'm trying to find Richard Allen Hematoxylin - specifically 7211 in Germany. Can anyone help me? Thanks. Jackie O' From collette2 <@t> mail.llnl.gov Mon Nov 10 11:22:46 2008 From: collette2 <@t> mail.llnl.gov (Nicole Collette) Date: Mon Nov 10 11:22:41 2008 Subject: [Histonet] lipid preservation in paraffin and mineralization of tissues? Message-ID: Hello, All, I am a postdoc with a couple of self-taught years of histology experience. I an about to attempt a protocol for preservation of lipid for paraffin sectioning as per Virchows Arch (2004) 445:22-26 Richard E. Tracy ? Parvene Walia Lipid fixation for fat staining in paraffin sections applied to lesions of atherosclerosis and A method to fix lipids for staining fat embolism in paraffin sections R E Tracy & P Walia Histopathology 2002, 41, 75-79 My question is whether a day at 2% chromic acid (24 h at 4 degrees C) is likely to decalcify the soft tissues? We are trying to look at atherosclerotic lesions including lipids (Oil Red O) and calcium deposits (von Kossa stain) in heart, liver, and kidney. Has anyone been able to perform RNA in situ or IHC on these samples after this type of processing? We do not have access to a cryostat. If anyone has tried this protocol with (or without) success, I'd be grateful for the advice. Thanks in advance, Nicole Collette LLNL/UCB collette2@llnl.gov From jkiernan <@t> uwo.ca Mon Nov 10 13:26:53 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Nov 10 13:26:57 2008 Subject: [Histonet] lipid preservation in paraffin and mineralization of tissues? Message-ID: Thank you Nicole, for bringing everyone's attention to these two publications. both describe the same technique of introducing highly unsaturated lipids into fatty materials already present in the tissue, and then rendering the unsaturated compounds insoluble in organic solvents by treatment with 2% aqueous chromium trioxide ("chromic acid") solution. The authors do not say why they used chromic acid for this purpose, and their only references are brief and incomplete quotations from books by Pearse and Lillie. The usual reagent for chromation of unsaturated lipids is not 2% chromic acid, which probably would remove pathological calcifications and RNA because its pH is 1.1. In lipid histochemistry, chromation is done with potassium dichromate, in solutions of pH 3.5 and higher. Baker's chromation method for formaldehyde-fixed blocks of tissue (Quart. J. Microsc. Sci. 87:441-470, 1946) consists of immersion in 5% potassium dichromate with 1% calcium chloride for 18h at room temperature, followed by 24h at 56-60C and then an overnight wash in running tap water. (The Ca ions were there to improve the precipitation of phospholipids.) In Elftman's "controlled chromation" (J Histochem. Cytochem. 2:1-8, 1954) the solution is 2.5% pot. dichromate at pH3.5 for 18h at 56C - conditions found to be optimal for insolubilizing phospholipids. The lipids may be stained either with a solvent dye such as Sudan black B or by virtue of the bound chromium(III) formed by reduction of dichromate. These are excellent methods for staining myelin and other phospholipid-rich structures such as mitochondria. The ingenious feature of the Tracey & Walia method is the initial infiltration with en emulsion of linoleic acid and phosphatidylcholine, which allows the localization of the site of any type of lipid by staining paraffin sections with a solvent dye (in their case, oil red O). One would expect the Tracey & Walia method to work even better with a rational chromation procedure than with chromic acid, and with less damage to sites of calcification. I do not know if enough mRNA would survive to allow in situ hybridization to sections of chromated tissue. If no-one else knows, you will just have to try it. John Kiernan Dept of Anatomy & Cell Biology University of Western Ontario London, Canada = = = ----- Original Message ----- From: Nicole Collette Date: Monday, November 10, 2008 12:23 Subject: [Histonet] lipid preservation in paraffin and mineralization of tissues? To: histonet@lists.utsouthwestern.edu > Hello, All, > > I am a postdoc with a couple of self-taught years > of histology experience. I an about to attempt a > protocol for preservation of lipid for paraffin > sectioning as per > > Virchows Arch (2004) 445:22-26 > Richard E. Tracy ? Parvene Walia > Lipid fixation for fat staining in paraffin sections applied > to lesions of atherosclerosis > > and > > A method to fix lipids for staining fat embolism > in paraffin sections > R E Tracy & P Walia > Histopathology 2002, 41, 75-79 > > My question is whether a day at 2% chromic acid > (24 h at 4 degrees C) is likely to decalcify the > soft tissues? We are trying to look at > atherosclerotic lesions including lipids (Oil Red > O) and calcium deposits (von Kossa stain) in > heart, liver, and kidney. Has anyone been able to > perform RNA in situ or IHC on these samples after > this type of processing? We do not have access to > a cryostat. If anyone has tried this protocol > with (or without) success, I'd be grateful for > the advice. > > Thanks in advance, > Nicole Collette > > LLNL/UCB > collette2@llnl.gov > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jo-ann.bader <@t> mcgill.ca Mon Nov 10 14:13:15 2008 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Mon Nov 10 14:13:32 2008 Subject: [Histonet] Histology Technician Position - Montreal Message-ID: The histology Core of the Goodman Cancer Centre at McGill Univrsity has an opening for a histology technician. It is an entry level position, some experience required in paraffin histology, cryostat preparations and cutting, routine and special stains. Experience in immunohistochemistry would be welcome as we are planning to add an immunohistochemistry area to the care facility. Please email you C.V.'s to jo-ann.bader@mcgill.ca Jo-Ann Bader Histology Facility Coordinator McGill Cancer Center Cancer Pavillion 1160 Pine Ave W Rm. 3355 Montreal, QC, Tel: 514-398-8270 Fax: 514-8398-6769 email: jo-ann.bader@mcgill.ca From gu.lang <@t> gmx.at Mon Nov 10 14:40:26 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Nov 10 14:41:01 2008 Subject: AW: [Histonet] Is there a Richard Allen Vendor in Europe? In-Reply-To: References: Message-ID: Hi Jackie, Microm sells the Richard Allen products. http://www.microm.de/microm%20homepage/microm_deutsch/html/faerbung.html bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jackie M O'Connor Gesendet: Montag, 10. November 2008 17:59 Cc: Histonet; histonet-bounces@lists.utsouthwestern.edu Betreff: [Histonet] Is there a Richard Allen Vendor in Europe? I'm trying to find Richard Allen Hematoxylin - specifically 7211 in Germany. Can anyone help me? Thanks. Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Mon Nov 10 16:25:29 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Nov 10 16:25:34 2008 Subject: [Histonet] Glassware Cleaner Message-ID: <57BE698966D5C54EAE8612E8941D7683042EC0F3@EXCHANGE3.huntingtonhospital.com> Can anyone recommend a glassware cleaner that is good for removing special stains (iron, mucicarmine, etc)? Laurie Colbert From jencres <@t> ca.rr.com Mon Nov 10 18:32:43 2008 From: jencres <@t> ca.rr.com (jennifer cresor mike hough) Date: Mon Nov 10 18:32:44 2008 Subject: [Histonet] Southern California salary ranges Message-ID: <15F59A44ADA7489A8E3B4C8A60FC23FA@jennifercresPC> Hello All, I just moved to the area and would like to get an idea of what the average salaries are. I am near Santa Anna and Riverside area. Any input would be appreciated. Thank you, Jennifer Cresor jencres@ca.rr.com From jkiernan <@t> uwo.ca Tue Nov 11 01:04:45 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Nov 11 01:04:51 2008 Subject: [Histonet] Trichrome Question Message-ID: Dear Angela Fogg, The staining method seems reasonable if the ingredients of your bought "Gomori trichrome" solution are the same as in Gomori's original method. You should check with the supplier and Gomori's paper. In your original Histonet post, you also wrote: > Fixed in 10% formalin for 3 weeks, processed > with formalin, alcoholic formalin, alcohols > and Clear Rite II ... Taken literally this means the specimens went from 10% formalin (= 4% formaldehyde) to 40% formaldehyde in water (formalin) and then into some formalin-alcohol mixture before being dehydrated in "alcohols". This isn't usually done. I don't know what Clear Rite II is - ? limonene, a mixture of hexanes & heptanes, or a glycol ether. There are many "xylene substitutes", mostly poorly described by the suppliers, and not all have the same miscibilities as xylene or toluene. The Histonet Archives contain many sob-stories about trade-secret clearing agents. For research, can you justify using any commercial product of unknown composition? No peer-reviewed journal will publish results that cannot be replicated. You also wrote: > ... embedded in EM400 paraffin ... Check the melting temperature of this product. Staining, especially by trichrome methods, is profoundly affected by the type of wax. An important paper about this was published by Allison & Bryant (1998) in Biotechnic & Histochemistry 73(3):128-136. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: angelafogg@aol.com Date: Monday, November 10, 2008 20:31 Subject: Re: [Histonet] Trichrome Question To: jkiernan@uwo.ca > The contents of this kit is as follows with the timing attached. > > Bouins for 1 hour at 60 degrees C > > Wash > > Gomori's One Step Trichrome for 15 minutes > > 1% Acetic acid for 1 min > > dehydate,clear etc > > Simple stain, easy to remember and works every time except for > this one piece of colon. > > Thanks, glad you are interested in assisting my quest to get an > answer. > Regards, > > Angela > > > > > > -----Original Message----- > > From: John Kiernan > > To: ANGELAFOGG@aol.com > > Cc: Histonet@lists.utsouthwestern.edu > > Sent: Mon, 10 Nov 2008 2:05 am > > Subject: Re: [Histonet] Trichrome Question > > > > > > Dear Angela Fogg, > > > > It is difficult to reply without knowing what's in the "Chromaview Stain kit Gomori's Trichrome." How does this differ from the method published by Gomori in Am. J. Clin. Path. 20: 662-664 (1950)? > > > > John Kiernan > > Anatomy, UWO > > London, Canada > > = = = > > ----- Original Message ----- > > From: ANGELAFOGG@aol.com > > Date: Saturday, November 8, 2008 10:03 > > Subject: Re: [Histonet] Trichrome Question > > To: jkiernan@uwo.ca > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > Thanks for responding. Fixed in 10% formalin for 3 weeks, processed > > > with formalin, alcoholic formalin, alcohols and Clear Rite III for Richard > > > Allan, embedded in EM400 paraffin from Surgipath. Cut at 5 microns and stained > > > using Richard Allan's Chromaview Stain kit Gomori's Trichrome. Used the > > > above method on our routine Trichrome controls and all the muscle stained red as > > > they are supposed to be. That's why I put out the question. The blue > > > staining of the muscle in the colon puzzled me. All patient tissue has > > > stained correctly as well. > > > > Thought maybe the length of time in formalin had something to do with the > > > blue staining. Maybe longer than an hour in Bouin's before continuing the > > > Trichrome would help? > > > > > Await your thoughts and info > > > > > Regards > > > > > Angela > > > > > > > > > > > > > In a message dated 11/8/2008 2:10:03 A.M. Eastern Standard Time, > > > jkiernan@uwo.ca writes: > > > > > > Which > > > trichrome method? What kind of section (frozen, paraffin, plastic?) > > > Any > > > post-fixation in something other than plain formalin before staining? > > > > > > Provide the technical details, and you will get lots of advice! > > > > > > > > > Weeks in formaldehyde is not "prolonged" fixation unless the > > > weeks > > > are numerous enough to make years. As a generality, trichrome > > > methods > > > do not work very well after fixation in liquids that have > > > formaldehyde as the > > > only active ingredient. Postfixation of the sections > > > can compensate. Bouin > > > is frequently used. Saturated aqueous picric acid us > > > just as good. There are > > > published reports that iodine and even citrate > > > buffer will improve trichrome > > > staining of paraffin sections of > > > formaldehyde-fixed tissue. See Yu & > > > Chapman 2003 J. > > > Histotechnol. 26(2): 131-134. > > > > > > > > > "Trichrome" has been applied to several staining > > > techniques that use two > > > or more dyes. By convention, since about 1920, > > > trichrome methods have > > > been those using phosphomolybdic or phosphotungstic > > > acid (or both) to > > > enable the staining of collagen and cytoplasm by > > > anionic dyes with sharply > > > contrasting colours: blue or green for > > > collagen, and red for cytoplasm > > > (including smooth & striated muscle). A > > > third anionic dye, typically yellow > > > or orange, may be added to stain red > > > blood cells. Instructions for > > > trichrome methods can be found in all > > > textbooks of microtechnique and > > > histotechnology. > > > > > > John > > > Kiernan > > > Anatomy, UWO > > > London, Canada > > > = = = > > > ----- Original Message > > > ----- > > > From: angelafogg@aol.com > > > Date: Friday, November 7, 2008 > > > 20:17 > > > Subject: [Histonet] Trichrome Question > > > To: > > > histonet@lists.utsouthwestern.edu > > > > > > > > > > > Performed a trichrome > > > stain on a piece of colon which had been > > > > in formalin for? weeks.? > > > Muscle stained blue instead of > > > > red.?What happened?? Does prolonged > > > fixation react this way? > > > > Hope someone can shed some light on > > > this. > > > > Regards, > > > > Angela > > > > > > > _______________________________________________ > > > > Histonet mailing > > > list > > > > Histonet@lists.utsouthwestern.edu > > > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > Instant access to the latest & most popular FREE games while you browse with the Games Toolbar - Download Now! > From nefff <@t> staff.uni-marburg.de Tue Nov 11 02:11:51 2008 From: nefff <@t> staff.uni-marburg.de (Dr. med. Frauke Neff) Date: Tue Nov 11 02:11:57 2008 Subject: [Histonet] CELL CULTURE LINE ON SLIDES In-Reply-To: References: <24A4826E8EF0964D86BC5317306F58A52BA2A07DE2@mmc-mail.ad.mhsil.com> Message-ID: <1226391111.49193e4736949@webmail.med.uni-marburg.de> Hi Jim, we use ice cold acetone for 5min. It seems to produce less background with our antibodies and usually don't need an extra permeabilisation step. My experience with 4% NBF was that the antibody thats supposed to stain intracellular vesicle doesn't like it;-) Good luck, Frauke -- Quoting Lee Crosby : > Jim, > I routinely stain cells with fluorescent antibodies. I fix them with 4% > Paraformaldehyde in PBS, or the easier way, which I use now, is in neutral > buffered formalin, which is readily purchased and does not require the labor > intensive steps of the former. > > Good luck. > > Lee Crosby > Echelon Biosciences Inc. > 801-588-0455 ext 329 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim > Sent: Monday, November 03, 2008 12:58 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] CELL CULTURE LINE ON SLIDES > > > We were asked to do immunostains on slides where a particular cell line was > growing. Can anyone tell me the best way to fix these slides before > performing immunostains? We used to use acetone on cytospins but can't > recall if there is a better way. > > thanks > > > > > Jim Vickroy BS, HT(ASCP) > Technical Supervisor - Surgical and Autopsy Pathology > Memorial Medical Center > 217-788-4046 > vickroy.jim@mhsil.com > > > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From vazquezr <@t> ohsu.edu Tue Nov 11 08:04:13 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Nov 11 08:04:26 2008 Subject: [Histonet] Glassware Cleaner In-Reply-To: <57BE698966D5C54EAE8612E8941D7683042EC0F3@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D7683042EC0F3@EXCHANGE3.huntingtonhospital.com> Message-ID: <2A582E8156B45F468A62D1F1D20AF083426E35@EX-BE08.ohsu.edu> Laurie, Just a guess, 1% acid alcohol or Micro 90. Robyn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Monday, November 10, 2008 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Glassware Cleaner Can anyone recommend a glassware cleaner that is good for removing special stains (iron, mucicarmine, etc)? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Tue Nov 11 09:32:58 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Nov 11 09:36:46 2008 Subject: [Histonet] RE: Glassware Cleaner In-Reply-To: <57BE698966D5C54EAE8612E8941D7683042EC0F3@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D7683042EC0F3@EXCHANGE3.huntingtonhospital.com> Message-ID: I had excellent results with a little concentrated hydrochloric acid. The first rinse could be dumped into a beaker full of sodium carbonate solution and washed down the drain. My wastewater people are currently happier with my scrubbing the glassware with Erado-Sol from Cambridge Diagnostics. It also works very well. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Monday, November 10, 2008 5:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Glassware Cleaner Can anyone recommend a glassware cleaner that is good for removing special stains (iron, mucicarmine, etc)? Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NHeath <@t> Lifespan.org Tue Nov 11 11:08:44 2008 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Tue Nov 11 11:09:45 2008 Subject: [Histonet] can anyone tell me Message-ID: <130E8991F210424096EFC6F42EA33B2403897DD7@LSCOEXCH1.lsmaster.lifespan.org> Hi, Can anyone tell me why you would put bone fragments in PennFix before decal?? Thanks :) From sross <@t> agh.org Tue Nov 11 11:17:11 2008 From: sross <@t> agh.org (Sarah Ross) Date: Tue Nov 11 11:17:30 2008 Subject: [Histonet] MEDITECH CATEGORIES FOR GYN Message-ID: <5D942CF4AE61B24E8DDF0DE22F282755223FBE@mse2.agh.org> We currently use Cerner and will be going live with Meditech client server on March 1st. Can any help me with setting up the dictionaries in Meditech for paps so we can print all of the high grades, atypical squamous cells, negatives, etc. In Cerner, all of our gyn alpha responses have a diagnostic category assigned to them (i.e. atypical squamous cells, atypical glandular cells, high grade SIL, etc) and also a case flag type (i.e. abnormal, normal, atypical, and unsatisfactory). Is this done in the prompt dictionary? Marker dictionary? Any screen shots/help would be greatly appreciated. Sarah Ross Pathology Dept Alpena Regional Medical Center sross@agh.org From rjbuesa <@t> yahoo.com Tue Nov 11 11:30:05 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 11 11:30:22 2008 Subject: [Histonet] can anyone tell me In-Reply-To: <130E8991F210424096EFC6F42EA33B2403897DD7@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: <887377.38678.qm@web65705.mail.ac4.yahoo.com> Because before you subject the bone fragments to the harsh decal treatment, the bone (and the BM it contains and the soft tissue they may have around) should be stabilized and "harden" by the fixative. Ren? J. --- On Tue, 11/11/08, Heath, Nancy L. wrote: From: Heath, Nancy L. Subject: [Histonet] can anyone tell me To: histonet@lists.utsouthwestern.edu Date: Tuesday, November 11, 2008, 12:08 PM Hi, Can anyone tell me why you would put bone fragments in PennFix before decal?? Thanks :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill.mcmanus <@t> usu.edu Tue Nov 11 13:09:15 2008 From: bill.mcmanus <@t> usu.edu (Bill McManus) Date: Tue Nov 11 13:09:24 2008 Subject: [Histonet] fluorescent labeling of starch Message-ID: <04BE433D-C8BA-4990-B809-33C6C7A49C81@usu.edu> Hello All: I am new to this group, but my "sig. nif other" has been on the list for many years. I am working with student that is trying to stain starches ( rice, potato, etc.) for confocal. Does anyone know of a way to label multiple starches, not just amylose but modified starches such as amylose pectin and other cross-linked starches with a single dye? If not, are there other multiple dyes that would be worth a try. We are not budgeted to produce antibodies at this time, maybe later. Bill McManus Western Dairy Center Utah State University bill.mcmanus@usu.edu From treese <@t> sleh.com Tue Nov 11 15:21:52 2008 From: treese <@t> sleh.com (Reese, Tommy G.) Date: Tue Nov 11 15:22:09 2008 Subject: [Histonet] (no subject) Message-ID: <023DBFA505E67A4689A2CEEC40AE3F7DA13B9B30EB@SLEHEXCH02.sleh.com> Continuing Education Oppurtunity ( 3 CE units ) Diagnostic Biosystems is sponsoring an immunochemistry workshop in cooperation with the Texas Society of Histotechnology. The workshop will be held at Houston Community College Coleman College for Health Sciences, Auditrium 1900 Pressler: Houston, Texas 77030 Date: Saturday, December 12th Time: 9:30-12:30 PM Title: IHC Basics and Beyond Maximum Attendance: 60 For more information or to RSVP, please contact Mary Daniel at mary.daniel@biosys.com, or Richard A. Breckenridge at Richard.Breckenridge@uth.tmc.edu +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. From amber.mckenzie <@t> gastrodocs.net Tue Nov 11 15:28:30 2008 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Nov 11 15:28:33 2008 Subject: [Histonet] lab chairs In-Reply-To: <023DBFA505E67A4689A2CEEC40AE3F7DA13B9B30EB@SLEHEXCH02.sleh.com> References: <023DBFA505E67A4689A2CEEC40AE3F7DA13B9B30EB@SLEHEXCH02.sleh.com> Message-ID: <03C921A1EAF7F541B16543F6EC6A4B370204FE42@giamail2.Gia.com> Where do you guys buy chairs for your lab? Do they have to be any certain type of chair? Amber From froyer <@t> bitstream.net Tue Nov 11 15:38:37 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Tue Nov 11 15:39:06 2008 Subject: [Histonet] lab chairs In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B370204FE42@giamail2.Gia.com> References: <023DBFA505E67A4689A2CEEC40AE3F7DA13B9B30EB@SLEHEXCH02.sleh.com> <03C921A1EAF7F541B16543F6EC6A4B370204FE42@giamail2.Gia.com> Message-ID: We sell exam stools, lab chairs/stools, etc. on my web site. Click on link below. On the home page, click on "Medical Equipment". Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 Web: http://www.minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, November 11, 2008 3:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lab chairs Where do you guys buy chairs for your lab? Do they have to be any certain type of chair? Amber _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Tue Nov 11 15:43:37 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Tue Nov 11 15:44:02 2008 Subject: [Histonet] lab chairs In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B370204FE42@giamail2.Gia.com> References: <023DBFA505E67A4689A2CEEC40AE3F7DA13B9B30EB@SLEHEXCH02.sleh.com> <03C921A1EAF7F541B16543F6EC6A4B370204FE42@giamail2.Gia.com> Message-ID: <4919FC89.8000800@umdnj.edu> > Where do you guys buy chairs for your lab? Do they have to be any certain type of chair? What type of chairs you buy is largely dependent on whether they supported Obama or McCain, and whether or not they know how to properly unsubscribe from this list! :-0 From FUNKM <@t> mercyhealth.com Tue Nov 11 16:56:53 2008 From: FUNKM <@t> mercyhealth.com (Marcia Funk) Date: Tue Nov 11 16:57:05 2008 Subject: [Histonet] CoPath users Message-ID: <4919B956.9B87.00AC.0@mercyhealth.com> I would really appreciate any feed back from folks that are using CoPath or other application off site for pathology transcription. Marcia From llewllew <@t> shaw.ca Tue Nov 11 17:17:40 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Nov 11 17:17:47 2008 Subject: [Histonet] fluorescent labeling of starch References: <04BE433D-C8BA-4990-B809-33C6C7A49C81@usu.edu> Message-ID: Try a PAS or a fluorescent PAS using Schiff's reagent made with acriflavine or acridine orange. Bryan Llewellyn ----- Original Message ----- From: "Bill McManus" To: Sent: Tuesday, November 11, 2008 11:09 AM Subject: [Histonet] fluorescent labeling of starch > Hello All: > > I am new to this group, but my "sig. nif other" has been on the list for > many years. I am working with student that is trying to stain starches > ( rice, potato, etc.) for confocal. Does anyone know of a way to label > multiple starches, not just amylose but modified starches such as amylose > pectin and other cross-linked starches with a single dye? If not, are > there other multiple dyes that would be worth a try. We are not budgeted > to produce antibodies at this time, maybe later. > > Bill McManus > Western Dairy Center > Utah State University > bill.mcmanus@usu.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Nov 12 02:15:21 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Nov 12 02:16:00 2008 Subject: [Histonet] can anyone tell me Message-ID: <86ADE4EB583CE64799A9924684A0FBBF05AB0E07@wahtntex2.waht.swest.nhs.uk> To fix them? The fixative 'protects' the cells from the harmful effects of the decalcifying fluid by stabilising the proteins. Also prevents the loss of some cellular components that could be soluble by creating a protein matrix that traps them. Basic premise of a fixative. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heath, Nancy L. Sent: 11 November 2008 17:09 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] can anyone tell me Hi, Can anyone tell me why you would put bone fragments in PennFix before decal?? Thanks :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NHeath <@t> Lifespan.org Wed Nov 12 05:40:38 2008 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Wed Nov 12 05:40:56 2008 Subject: [Histonet] can anyone tell me... Message-ID: <130E8991F210424096EFC6F42EA33B2403897E2C@LSCOEXCH1.lsmaster.lifespan.org> Hi Everyone :) Thanks to all for answering my question about why you would put bone in PennFix before decal :) Maybe I should have written the question a bit differently. I know bone is to be well fixed before decal...I just wanted to know why someone would put bone in Pennfix versus 10% neutral buffered formalin??? Is there any difference or extra benefits with PennFix?? Thanks From rjbuesa <@t> yahoo.com Wed Nov 12 07:41:39 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Nov 12 07:41:42 2008 Subject: [Histonet] can anyone tell me... In-Reply-To: <130E8991F210424096EFC6F42EA33B2403897E2C@LSCOEXCH1.lsmaster.lifespan.org> Message-ID: <5779.70861.qm@web65701.mail.ac4.yahoo.com> Nancy: Well this is a different question altogether, you are wondering about if you should use Penfix versus NBF (you should never use unbuffered 10% formalin). Penfix is a commercial mixture of less than 10% formalin + methanol + ethanol + 2-propanol (3 different alcohols) in undisclosed (proprietary amounts) never evaluated independently (meaning that the only "evaluation" was done by the manufacturer). It has been said (anecdotalal information) that "overfixes" and dries small biopsies, something understandable due to the alcohols it contains. Since alcohols and formalin fix tissues in very different ways I personally do not see any advantage (and I could think on some disadvantages) of this "combined" fixation. I personally would NOT use it. Now it is after you.Ren? J. --- On Wed, 11/12/08, Heath, Nancy L. wrote: From: Heath, Nancy L. Subject: [Histonet] can anyone tell me... To: histonet@lists.utsouthwestern.edu Date: Wednesday, November 12, 2008, 6:40 AM Hi Everyone :) Thanks to all for answering my question about why you would put bone in PennFix before decal :) Maybe I should have written the question a bit differently. I know bone is to be well fixed before decal...I just wanted to know why someone would put bone in Pennfix versus 10% neutral buffered formalin??? Is there any difference or extra benefits with PennFix?? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Nov 12 07:48:28 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Nov 12 07:48:45 2008 Subject: [Histonet] can anyone tell me... In-Reply-To: <5779.70861.qm@web65701.mail.ac4.yahoo.com> References: <130E8991F210424096EFC6F42EA33B2403897E2C@LSCOEXCH1.lsmaster.lifespan.org> <5779.70861.qm@web65701.mail.ac4.yahoo.com> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C4D9@LTA3VS011.ees.hhs.gov> Found this on Histosearch. Also, when I was in a hospital lab we used it all the time on breast and any fatty tissues with great success. http://www.histosearch.com/histonet/Jan00/Penfix.html Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, November 12, 2008 8:42 AM To: histonet@lists.utsouthwestern.edu; Heath, Nancy L. Subject: Re: [Histonet] can anyone tell me... Nancy: Well this is a different question altogether, you are wondering about if you should use Penfix versus NBF (you should never use unbuffered 10% formalin). Penfix is a commercial mixture of less than 10% formalin + methanol + ethanol + 2-propanol (3 different alcohols) in undisclosed (proprietary amounts) never evaluated independently (meaning that the only "evaluation" was done by the manufacturer). It has been said (anecdotalal information) that "overfixes" and dries small biopsies, something understandable due to the alcohols it contains. Since alcohols and formalin fix tissues in very different ways I personally do not see any advantage (and I could think on some disadvantages) of this "combined" fixation. I personally would NOT use it. Now it is after you.Ren? J. --- On Wed, 11/12/08, Heath, Nancy L. wrote: From: Heath, Nancy L. Subject: [Histonet] can anyone tell me... To: histonet@lists.utsouthwestern.edu Date: Wednesday, November 12, 2008, 6:40 AM Hi Everyone :) Thanks to all for answering my question about why you would put bone in PennFix before decal :) Maybe I should have written the question a bit differently. I know bone is to be well fixed before decal...I just wanted to know why someone would put bone in Pennfix versus 10% neutral buffered formalin??? Is there any difference or extra benefits with PennFix?? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Nov 12 08:16:33 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Nov 12 08:16:39 2008 Subject: [Histonet] can anyone tell me... Message-ID: <86ADE4EB583CE64799A9924684A0FBBF05AB0EC2@wahtntex2.waht.swest.nhs.uk> So it is 10% formol alcohol in 70% alcohol; wonder what the other 20% is? Water? Alcoholic fixatives as a genre tend to overharden on standing but I guess fatty tissue would benefit. Why would anyone use something you don't actually know what it is composed of? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 12 November 2008 13:48 To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; Heath, Nancy L. Subject: RE: [Histonet] can anyone tell me... Found this on Histosearch. Also, when I was in a hospital lab we used it all the time on breast and any fatty tissues with great success. http://www.histosearch.com/histonet/Jan00/Penfix.html Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, November 12, 2008 8:42 AM To: histonet@lists.utsouthwestern.edu; Heath, Nancy L. Subject: Re: [Histonet] can anyone tell me... Nancy: Well this is a different question altogether, you are wondering about if you should use Penfix versus NBF (you should never use unbuffered 10% formalin). Penfix is a commercial mixture of less than 10% formalin + methanol + ethanol + 2-propanol (3 different alcohols) in undisclosed (proprietary amounts) never evaluated independently (meaning that the only "evaluation" was done by the manufacturer). It has been said (anecdotalal information) that "overfixes" and dries small biopsies, something understandable due to the alcohols it contains. Since alcohols and formalin fix tissues in very different ways I personally do not see any advantage (and I could think on some disadvantages) of this "combined" fixation. I personally would NOT use it. Now it is after you.Ren? J. --- On Wed, 11/12/08, Heath, Nancy L. wrote: From: Heath, Nancy L. Subject: [Histonet] can anyone tell me... To: histonet@lists.utsouthwestern.edu Date: Wednesday, November 12, 2008, 6:40 AM Hi Everyone :) Thanks to all for answering my question about why you would put bone in PennFix before decal :) Maybe I should have written the question a bit differently. I know bone is to be well fixed before decal...I just wanted to know why someone would put bone in Pennfix versus 10% neutral buffered formalin??? Is there any difference or extra benefits with PennFix?? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Wed Nov 12 08:29:17 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Nov 12 08:29:23 2008 Subject: [Histonet] lab chairs In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B370204FE42@giamail2.Gia.com> Message-ID: <000201c944d3$0b5fdd70$d00f7ca5@lurie.northwestern.edu> I think you will be fine with whatever you want as long as it not cloth. We use the bio-fit chairs. We get them from VWR. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, November 11, 2008 3:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lab chairs Where do you guys buy chairs for your lab? Do they have to be any certain type of chair? Amber _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From elizabeth.a.mcneil <@t> gsk.com Wed Nov 12 08:35:29 2008 From: elizabeth.a.mcneil <@t> gsk.com (elizabeth.a.mcneil@gsk.com) Date: Wed Nov 12 08:36:00 2008 Subject: [Histonet] Lab Chairs Message-ID: Hi Amber, I would seriously look at it from the perspective of use...our lab went to an ergonomic chair because of the hours spent in the chairs performing our daily histology task... Elizabeth A McNeil SA Histology 919 483 4033 From gu.lang <@t> gmx.at Wed Nov 12 09:44:54 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Nov 12 09:45:14 2008 Subject: [Histonet] experiences with kappa/lambda Message-ID: <4E6C71CA61B34519B5EB7451A0A52813@dielangs.at> Hi listmembers, Please tell me about your experiences with the IHC-demonstration of kappa and lambda on FFPE-sections. We have some troubles about inconsistend staining and therefore false results about the monoclonality of tumors. Bye Gudrun From JWeems <@t> sjha.org Wed Nov 12 10:24:35 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Nov 12 10:24:48 2008 Subject: [Histonet] experiences with kappa/lambda In-Reply-To: <4E6C71CA61B34519B5EB7451A0A52813@dielangs.at> References: <4E6C71CA61B34519B5EB7451A0A52813@dielangs.at> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA5127943@ITSSSXM01V6.one.ads.che.org> Our docs liked the alk phos best, but still were not happy until ISH came along. We are bringing that in house now - in the process of looking at the Leica Bond. The docs are happy! Best, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From GDawson <@t> dynacaremilwaukee.com Wed Nov 12 10:27:15 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Nov 12 10:27:27 2008 Subject: [Histonet] experiences with kappa/lambda In-Reply-To: <4E6C71CA61B34519B5EB7451A0A52813@dielangs.at> Message-ID: Gudrun, In my experience, Kappa & Lambda IHC staining tops my list of difficult antibodies. Consistency was always poor & I've tried MANY of the available kappa/lambda antibodies out there (both polyclonal & monoclonal). In situ hybridization is the way to go as I believe that IHC demonstration of these two antibodies will always be sub-standard. My Opinion, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gudrun Lang Sent: Wednesday, November 12, 2008 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] experiences with kappa/lambda Hi listmembers, Please tell me about your experiences with the IHC-demonstration of kappa and lambda on FFPE-sections. We have some troubles about inconsistend staining and therefore false results about the monoclonality of tumors. Bye Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Wed Nov 12 10:58:23 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Nov 12 10:58:26 2008 Subject: [Histonet] experiences with kappa/lambda In-Reply-To: <4E6C71CA61B34519B5EB7451A0A52813@dielangs.at> References: <4E6C71CA61B34519B5EB7451A0A52813@dielangs.at> Message-ID: <5b6eb13e0811120858g1b4e5f30wba188fd00d283f8f@mail.gmail.com> Kappa/Lambda has to be titered to the patient's sample sometimes. Staining consistency is hard to acheive, even when you use the same protocol each day. When I think of all the ugly Kappa/Lambda slides I've stained...yuck On Wed, Nov 12, 2008 at 7:44 AM, Gudrun Lang wrote: > Hi listmembers, > > Please tell me about your experiences with the IHC-demonstration of kappa > and lambda on FFPE-sections. > > We have some troubles about inconsistend staining and therefore false > results about the monoclonality of tumors. > > Bye > > Gudrun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mbisher <@t> Princeton.EDU Wed Nov 12 11:02:29 2008 From: mbisher <@t> Princeton.EDU (Peggy Bisher) Date: Wed Nov 12 11:02:34 2008 Subject: [Histonet] Plant Tissue Processing Message-ID: I have a professor here who would like me to make 10-15 micron thick sections of lawn grass. I thought the best way to do this would be paraffin embedding. Does anybody have any suggestions as to the time/types for my paraffin tissue processor? I am not very familiar working with plant material. Thank you very much, Maggie Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher@princeton.edu From Erin.Martin <@t> ucsf.edu Wed Nov 12 11:04:49 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Wed Nov 12 11:05:01 2008 Subject: [Histonet] NGF p75 Message-ID: Good morning, Does anyone know of a source for antibody NGF p75 other than Chemicon/Millipore? The last few lots we have gotten from them have been dramatically weaker so I'd like to find a new vendor. Thanks! Erin From dont8know8me <@t> yahoo.com Wed Nov 12 11:13:16 2008 From: dont8know8me <@t> yahoo.com (richard peralta) Date: Wed Nov 12 11:13:20 2008 Subject: [Histonet] (no subject) Message-ID: <440761.84760.qm@web51405.mail.re2.yahoo.com> hi histonetters, ? anyone can tell me whats the best control for MYOGENIN ....were using heart muscle and muscle from the leg...but unfortunately our stain still not working right......any other control tissue that we can try? ? suggestion is deeply apreciated....ty From gu.lang <@t> gmx.at Wed Nov 12 11:58:28 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Nov 12 11:58:49 2008 Subject: AW: [Histonet] (no subject) In-Reply-To: <440761.84760.qm@web51405.mail.re2.yahoo.com> References: <440761.84760.qm@web51405.mail.re2.yahoo.com> Message-ID: <01B4A0DDD2B3468D84A105F7AF493586@dielangs.at> Control-tissue for myogenin is rhabdomyosarkom. Normal muscle hardly shows a staining. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von richard peralta Gesendet: Mittwoch, 12. November 2008 18:13 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] (no subject) hi histonetters, ? anyone can tell me whats the best control for MYOGENIN ....were using heart muscle and muscle from the leg...but unfortunately our stain still not working right......any other control tissue that we can try? ? suggestion is deeply apreciated....ty _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Nov 12 12:00:23 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Nov 12 12:00:41 2008 Subject: [Histonet] kappa/lambda and.. In-Reply-To: <4E6C71CA61B34519B5EB7451A0A52813@dielangs.at> References: <4E6C71CA61B34519B5EB7451A0A52813@dielangs.at> Message-ID: <01F4C23B139344A58D76932541547D8D@dielangs.at> Thank you for your answers. An additional question: Can someone confirm, that underfixed tissue shows false positive kappa and lambdastaining? Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Mittwoch, 12. November 2008 16:45 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] experiences with kappa/lambda Hi listmembers, Please tell me about your experiences with the IHC-demonstration of kappa and lambda on FFPE-sections. We have some troubles about inconsistend staining and therefore false results about the monoclonality of tumors. Bye Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed Nov 12 12:04:54 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Nov 12 12:05:08 2008 Subject: [Histonet] (no subject) In-Reply-To: <440761.84760.qm@web51405.mail.re2.yahoo.com> References: <440761.84760.qm@web51405.mail.re2.yahoo.com> Message-ID: <491AD4760200007700006CD6@gwmail4.harthosp.org> We use a rhabdomyosarcoma. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> richard peralta 11/12/08 12:13 PM >>> hi histonetters, anyone can tell me whats the best control for MYOGENIN ....were using heart muscle and muscle from the leg...but unfortunately our stain still not working right......any other control tissue that we can try? suggestion is deeply apreciated....ty _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Wed Nov 12 12:07:14 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Nov 12 12:07:25 2008 Subject: [Histonet] experiences with kappa/lambda In-Reply-To: <4E6C71CA61B34519B5EB7451A0A52813@dielangs.at> Message-ID: Hi Gudrun. We have a fair bit of success with kappa/lambda IHC. We use polyclonal antibodies, a heat citrate pretreatment. The caveat is that we have several different titers in use - we have a titer for lymph nodes, one for bone marrows/decaled tissue, one for tissue fixed in zinc formalin, etc.... We have pretty good correlation with our flow lab. I believe that a couple of years ago Rich Cartun published a paper on his methods & he has good correlation, too. It does require a pathologist with good knowledge of heme path & patience to work out the optimum staining with the lab. Patti Loykasek BS, HTL, QIHC Clinical IHC Supervisor PhenoPath Laboratories Seattle, WA > Hi listmembers, > > Please tell me about your experiences with the IHC-demonstration of kappa > and lambda on FFPE-sections. > > We have some troubles about inconsistend staining and therefore false > results about the monoclonality of tumors. > > Bye > > Gudrun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From ploykasek <@t> phenopath.com Wed Nov 12 12:07:58 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Nov 12 12:08:07 2008 Subject: [Histonet] ERCC1 antibody Message-ID: Hi all. Just wondering if someone would share what antibody they are using for ERCC1. We have used clone 8f1 in the past, but was wondering if perhaps there is a new, better clone. I haven't been impressed with the staining that we have. Thanks. Patti Loykasek BS, HTL, QIHC Clinical IHC Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From ploykasek <@t> phenopath.com Wed Nov 12 12:09:41 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Nov 12 12:09:50 2008 Subject: [Histonet] (no subject) In-Reply-To: <440761.84760.qm@web51405.mail.re2.yahoo.com> Message-ID: Fetal limb tissue is positive for myogenin. Patti Loykasek > hi histonetters, > ? > anyone can tell me whats the best control for MYOGENIN ....were using heart > muscle and muscle from the leg...but unfortunately our stain still not working > right......any other control tissue that we can try? > ? > suggestion is deeply apreciated....ty > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From burch007 <@t> mc.duke.edu Wed Nov 12 12:41:51 2008 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Wed Nov 12 12:42:03 2008 Subject: [Histonet] NGF p75 In-Reply-To: Message-ID: Leica Microsystems # NCL-NGFR "Martin, Erin" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/12/2008 12:06 PM To histonet cc Subject [Histonet] NGF p75 Good morning, Does anyone know of a source for antibody NGF p75 other than Chemicon/Millipore? The last few lots we have gotten from them have been dramatically weaker so I'd like to find a new vendor. Thanks! Erin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pinetree110567 <@t> yahoo.com Wed Nov 12 12:46:21 2008 From: pinetree110567 <@t> yahoo.com (Jack Cates) Date: Wed Nov 12 12:46:26 2008 Subject: [Histonet] RA Lamb has been sold to the evil empire Message-ID: <530032.77007.qm@web44916.mail.sp1.yahoo.com> I am very sorry to see this happen again to a great company who bent over backwards to assist customers.? I hope ThemoShandonFisherRichardRaymond (to be continued) will start to keep the parts that made Richard Allen, Raymond A. Lamb and others great to work with I am also very sorry to see Tonia and Jerry go. Tuesday, Nov. 11, 2008 Thermo Fisher Scientific Strengthens Anatomical Pathology Portfolio with Acquisition of Raymond A. Lamb WALTHAM, Mass. ? Thermo Fisher Scientific Inc., the world leader in serving science, today announced it has acquired Raymond A. Lamb Ltd., a manufacturer of histology and anatomical pathology products based in Eastbourne, U.K., near London. In business since 1964, Raymond A. Lamb has grown to become a global provider of products for the pathology laboratory. Recently, its focus has been on systems that automate the labeling and tracking of slides and cassettes that carry patient tissue samples, reducing the possibility of errors during their processing, storage and retrieval. The company has had a longstanding relationship with the anatomical pathology business of Thermo Fisher as a supplier of labeling products for cassettes and glass slides, as well as other anatomical pathology equipment. "Our anatomical pathology customers - in both clinical and research markets - are demanding better solutions for tracking the increasing number of samples that they need to process and diagnose," said Marijn E. Dekkers, president and chief executive officer of Thermo Fisher Scientific. "This acquisition brings new-generation systems that can be integrated with our existing portfolio of anatomical pathology equipment and consumables to create a more efficient, and reliable, workflow." Raymond A. Lamb had revenues of approximately $9 million in 2007, and will be integrated into Thermo Fisher's Analytical Technologies Segment. About Thermo Fisher Scientific Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With annual revenues of $10 billion, we have more than 30,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. Thermo Scientific offers customers a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com. Advertisement The following constitutes a "Safe Harbor" statement under the Private Securities Litigation Reform Act of 1995: This press release contains forward-looking statements that involve a number of risks and uncertainties. Important factors that could cause actual results to differ materially from those indicated by such forward-looking statements are set forth in the company's Quarterly Report on Form 10-Q for the quarter ended September 27, 2008, under the caption "Risk Factors," which is on file with the Securities and Exchange Commission (SEC) and available in the "Investors" section of our Website under the heading "SEC Filings." Important factors that could cause actual results to differ materially from those indicated by forward-looking statements include risks and uncertainties relating to: competition and its effect on pricing, spending, third-party relationships and revenues; the need to develop new products and adapt to significant technological change; implementation of strategies for improving internal growth; general worldwide economic conditions and related uncertainties; use and protection of intellectual property; dependence on customers' capital spending policies and government funding policies; realization of potential future savings from new productivity initiatives; the effect of changes in governmental regulations; the effect of exchange rate fluctuations on international operations; the effect of laws and regulations governing government contracts; the effect of competing with certain of our customers and suppliers; and the effect of rapid changes in the healthcare industry. While we may elect to update forward-looking statements at some point in the future, we specifically disclaim any obligation to do so, even if our estimates change and, therefore, you should not rely on these forward-looking statements as representing our views as of any date subsequent to to From marktarango <@t> gmail.com Wed Nov 12 12:48:02 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Nov 12 12:48:06 2008 Subject: [Histonet] kappa/lambda and.. In-Reply-To: <01F4C23B139344A58D76932541547D8D@dielangs.at> References: <4E6C71CA61B34519B5EB7451A0A52813@dielangs.at> <01F4C23B139344A58D76932541547D8D@dielangs.at> Message-ID: <5b6eb13e0811121048p681d59cbp9d9708db6bead9b7@mail.gmail.com> I"ve seen underfixed tissue stain with an ugly background everywhere for kappa/lambda (so much that you can't tell what's real staining and what isn't). I think it depends on your retrieval. Especially if you're digesting for retrieval, and it wasn't properly fixed, I can imagine all kinds of staining issues. On Wed, Nov 12, 2008 at 10:00 AM, Gudrun Lang wrote: > Thank you for your answers. An additional question: Can someone confirm, > that underfixed tissue shows false positive kappa and lambdastaining? > > Bye > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun > Lang > Gesendet: Mittwoch, 12. November 2008 16:45 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] experiences with kappa/lambda > > Hi listmembers, > > Please tell me about your experiences with the IHC-demonstration of kappa > and lambda on FFPE-sections. > > We have some troubles about inconsistend staining and therefore false > results about the monoclonality of tumors. > > Bye > > Gudrun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Wendy <@t> medequipsource.com Wed Nov 12 13:12:19 2008 From: Wendy <@t> medequipsource.com (Wendy Mascio) Date: Wed Nov 12 13:12:23 2008 Subject: [Histonet] RE: Service company Message-ID: <71A36250328DA34495AA0F972AE170C8111B7F@sbs01.MedicalEquipment.local> Has anyone had any dealings with Southern Medical Services? I was wondering what your thoughts are on this company. Our service was OK but the office staff, billing etc has been a nightmare. From carl.hobbs <@t> kcl.ac.uk Wed Nov 12 13:24:30 2008 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Wed Nov 12 13:25:54 2008 Subject: [Histonet] re: NGFrp75 Message-ID: <11D9615B89C10747B1C985966A63D7CA286ECA890F@KCL-MAIL04.kclad.ds.kcl.ac.uk> Hi Erin. You don't state what the I.D. of the Chemicon Ab is, nor the species / tissue prep. you are using. I have compared Chemicon's AB1554 with Sigma's N3908 ( on rat and mouse Formalin-fixed P.wax sections). Also, Novocastra have an anti human NGFR ( gp75) : NCL-NGFR, which I have not tested as it is stated as being human-specific. I hope that I interpreted your request correctly. carl From Aubrey <@t> nsh.org Wed Nov 12 13:32:16 2008 From: Aubrey <@t> nsh.org (Aubrey Wanner) Date: Wed Nov 12 13:32:26 2008 Subject: [Histonet] NSH Lunch & Learn November 15th Message-ID: Limited spaces are still available for the NSH Fall Lunch & Learn, presented in partnership with Leica Microsystems, taking place November 15, 2008 at Harford Community College in Bel Air, MD. Attendees have an opportunity to attend 3 sessions and earn 4.5 contact hours in this half day program. Schedule: 8:00am - 9:00am: Registration 9:00am - 10:30am: Basic Dynamics of Fixation & Processing 10:30am - 12:00am: Small Specimen Management - In a Large Volume World 12:00pm - 12:30pm: Lunch Provided by NSH 12:30pm - 2:00pm: The Paradox of Change in Histotechnology All sessions are presented by top rated NSH presenter Skip Brown, Clinical Applications Manager, Leica BioSystems L.L.C.-St. Louis Its a great inexpensive way to expand your knowledge & earn those needed contact hours. Visit www.nsh.org to register. Mrs. Aubrey M.J. Wanner Meeting Manager National Society for Histotechnology 10320 Little Patuxent Parkway | Suite 804 Columbia, MD 21044 Main | 443.535.4060 Direct | 443.535.4065 Fax | 443.535.4055 http://www.nsh.org/ Looking for continuing education? Visit the NSH website . P Please consider the environment before printing this email. From integrated.histo <@t> gmail.com Wed Nov 12 13:44:50 2008 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Wed Nov 12 13:44:56 2008 Subject: [Histonet] 4 Day Weekend Message-ID: <5d9104a30811121144oe37d395of901abeb67cad900@mail.gmail.com> For the first time in my histo career, our lab will be closed the day after Thanksgiving. I am concerned about tissue sitting in the processors (in formalin) for that long. What are other labs doing? Cindy DuBois Integrated Pathology Stockton, CA From lblazek <@t> digestivespecialists.com Wed Nov 12 13:56:15 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Nov 12 13:54:44 2008 Subject: [Histonet] 4 Day Weekend In-Reply-To: <5d9104a30811121144oe37d395of901abeb67cad900@mail.gmail.com> References: <5d9104a30811121144oe37d395of901abeb67cad900@mail.gmail.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E3907BE7D29E2@IBMB7Exchange.digestivespecialists.com> Working on Friday! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Wednesday, November 12, 2008 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 4 Day Weekend For the first time in my histo career, our lab will be closed the day after Thanksgiving. I am concerned about tissue sitting in the processors (in formalin) for that long. What are other labs doing? Cindy DuBois Integrated Pathology Stockton, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Wed Nov 12 14:12:29 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Nov 12 14:12:50 2008 Subject: [Histonet] 4 Day Weekend In-Reply-To: <5d9104a30811121144oe37d395of901abeb67cad900@mail.gmail.com> Message-ID: If it's antigenicity you're concerned with (we had a 48 hour max fix time) - set your processor to transfer the tissues to 70% alcohol and hold them there until time to process for Monday a.m. Ta-daa! "Cindy DuBois" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/12/2008 01:44 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] 4 Day Weekend For the first time in my histo career, our lab will be closed the day after Thanksgiving. I am concerned about tissue sitting in the processors (in formalin) for that long. What are other labs doing? Cindy DuBois Integrated Pathology Stockton, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Wed Nov 12 14:37:05 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Nov 12 14:37:11 2008 Subject: [Histonet] Re: experiences with kappa/lambda Message-ID: I just had an extraordinary experience with kappa and lambda light chain staining, or rather the lack thereof. I didn't get to see the IHC slides, which were prepared at a reference laboratory (well, to give them due credit, by Vanderbilt's hematopathology division). I saw an odd-looking lymphoma in an older woman, and sent the material to Vanderbilt for immunostains. (Unfortunately I could not prepare imprints or obtain flow cytometry, since I received the specimen already in formalin.) Vandy did the necessary immunostains, and could not see either kappa and lambda marking. IHC for IgG heavy chain was however positive. Cells showing plasmacytoid differentiation marked for CD138, while more lymphocyte-like tumor cells marked for CD20 and other B-cell markers. Their diagnosis of gamma heavy chain disease was borne out by subsequent serum protein studies (I haven't seen those reports.) Apparently they tumbled to the diagnosis because of the lack of light chain marking in a tumor that seemed obviously clonal. Impressive! (Furthermore, Vandy's favored to kick UT's butt a week from Saturday.) Bob Richmond Samurai Pathologist Knoxville TN From b-frederick <@t> northwestern.edu Wed Nov 12 14:46:45 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Nov 12 14:46:58 2008 Subject: [Histonet] RA Lamb has been sold to the evil empire In-Reply-To: <530032.77007.qm@web44916.mail.sp1.yahoo.com> Message-ID: <000301c94507$c9d3fa50$d00f7ca5@lurie.northwestern.edu> I keep telling everyone it's going to be "histosupplies are us" one of these days!!! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Cates Sent: Wednesday, November 12, 2008 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RA Lamb has been sold to the evil empire I am very sorry to see this happen again to a great company who bent over backwards to assist customers.? I hope ThemoShandonFisherRichardRaymond (to be continued) will start to keep the parts that made Richard Allen, Raymond A. Lamb and others great to work with I am also very sorry to see Tonia and Jerry go. Tuesday, Nov. 11, 2008 Thermo Fisher Scientific Strengthens Anatomical Pathology Portfolio with Acquisition of Raymond A. Lamb WALTHAM, Mass. ? Thermo Fisher Scientific Inc., the world leader in serving science, today announced it has acquired Raymond A. Lamb Ltd., a manufacturer of histology and anatomical pathology products based in Eastbourne, U.K., near London. In business since 1964, Raymond A. Lamb has grown to become a global provider of products for the pathology laboratory. Recently, its focus has been on systems that automate the labeling and tracking of slides and cassettes that carry patient tissue samples, reducing the possibility of errors during their processing, storage and retrieval. The company has had a longstanding relationship with the anatomical pathology business of Thermo Fisher as a supplier of labeling products for cassettes and glass slides, as well as other anatomical pathology equipment. "Our anatomical pathology customers - in both clinical and research markets - are demanding better solutions for tracking the increasing number of samples that they need to process and diagnose," said Marijn E. Dekkers, president and chief executive officer of Thermo Fisher Scientific. "This acquisition brings new-generation systems that can be integrated with our existing portfolio of anatomical pathology equipment and consumables to create a more efficient, and reliable, workflow." Raymond A. Lamb had revenues of approximately $9 million in 2007, and will be integrated into Thermo Fisher's Analytical Technologies Segment. About Thermo Fisher Scientific Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With annual revenues of $10 billion, we have more than 30,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. Thermo Scientific offers customers a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com. Advertisement The following constitutes a "Safe Harbor" statement under the Private Securities Litigation Reform Act of 1995: This press release contains forward-looking statements that involve a number of risks and uncertainties. Important factors that could cause actual results to differ materially from those indicated by such forward-looking statements are set forth in the company's Quarterly Report on Form 10-Q for the quarter ended September 27, 2008, under the caption "Risk Factors," which is on file with the Securities and Exchange Commission (SEC) and available in the "Investors" section of our Website under the heading "SEC Filings." Important factors that could cause actual results to differ materially from those indicated by forward-looking statements include risks and uncertainties relating to: competition and its effect on pricing, spending, third-party relationships and revenues; the need to develop new products and adapt to significant technological change; implementation of strategies for improving internal growth; general worldwide economic conditions and related uncertainties; use and protection of intellectual property; dependence on customers' capital spending policies and government funding policies; realization of potential future savings from new productivity initiatives; the effect of changes in governmental regulations; the effect of exchange rate fluctuations on international operations; the effect of laws and regulations governing government contracts; the effect of competing with certain of our customers and suppliers; and the effect of rapid changes in the healthcare industry. While we may elect to update forward-looking statements at some point in the future, we specifically disclaim any obligation to do so, even if our estimates change and, therefore, you should not rely on these forward-looking statements as representing our views as of any date subsequent to to _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Wed Nov 12 14:53:53 2008 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Wed Nov 12 14:53:59 2008 Subject: [Histonet] RA Lamb has been sold to the evil empire In-Reply-To: <000301c94507$c9d3fa50$d00f7ca5@lurie.northwestern.edu> References: <530032.77007.qm@web44916.mail.sp1.yahoo.com> <000301c94507$c9d3fa50$d00f7ca5@lurie.northwestern.edu> Message-ID: <003d01c94508$c5aec530$095a5b82@vet.upenn.edu> It may become "Buy here or get nothing" Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, November 12, 2008 3:47 PM To: 'Jack Cates'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RA Lamb has been sold to the evil empire I keep telling everyone it's going to be "histosupplies are us" one of these days!!! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Cates Sent: Wednesday, November 12, 2008 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RA Lamb has been sold to the evil empire I am very sorry to see this happen again to a great company who bent over backwards to assist customers.? I hope ThemoShandonFisherRichardRaymond (to be continued) will start to keep the parts that made Richard Allen, Raymond A. Lamb and others great to work with I am also very sorry to see Tonia and Jerry go. Tuesday, Nov. 11, 2008 Thermo Fisher Scientific Strengthens Anatomical Pathology Portfolio with Acquisition of Raymond A. Lamb WALTHAM, Mass. ? Thermo Fisher Scientific Inc., the world leader in serving science, today announced it has acquired Raymond A. Lamb Ltd., a manufacturer of histology and anatomical pathology products based in Eastbourne, U.K., near London. In business since 1964, Raymond A. Lamb has grown to become a global provider of products for the pathology laboratory. Recently, its focus has been on systems that automate the labeling and tracking of slides and cassettes that carry patient tissue samples, reducing the possibility of errors during their processing, storage and retrieval. The company has had a longstanding relationship with the anatomical pathology business of Thermo Fisher as a supplier of labeling products for cassettes and glass slides, as well as other anatomical pathology equipment. "Our anatomical pathology customers - in both clinical and research markets - are demanding better solutions for tracking the increasing number of samples that they need to process and diagnose," said Marijn E. Dekkers, president and chief executive officer of Thermo Fisher Scientific. "This acquisition brings new-generation systems that can be integrated with our existing portfolio of anatomical pathology equipment and consumables to create a more efficient, and reliable, workflow." Raymond A. Lamb had revenues of approximately $9 million in 2007, and will be integrated into Thermo Fisher's Analytical Technologies Segment. About Thermo Fisher Scientific Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With annual revenues of $10 billion, we have more than 30,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. Thermo Scientific offers customers a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com. Advertisement The following constitutes a "Safe Harbor" statement under the Private Securities Litigation Reform Act of 1995: This press release contains forward-looking statements that involve a number of risks and uncertainties. Important factors that could cause actual results to differ materially from those indicated by such forward-looking statements are set forth in the company's Quarterly Report on Form 10-Q for the quarter ended September 27, 2008, under the caption "Risk Factors," which is on file with the Securities and Exchange Commission (SEC) and available in the "Investors" section of our Website under the heading "SEC Filings." Important factors that could cause actual results to differ materially from those indicated by forward-looking statements include risks and uncertainties relating to: competition and its effect on pricing, spending, third-party relationships and revenues; the need to develop new products and adapt to significant technological change; implementation of strategies for improving internal growth; general worldwide economic conditions and related uncertainties; use and protection of intellectual property; dependence on customers' capital spending policies and government funding policies; realization of potential future savings from new productivity initiatives; the effect of changes in governmental regulations; the effect of exchange rate fluctuations on international operations; the effect of laws and regulations governing government contracts; the effect of competing with certain of our customers and suppliers; and the effect of rapid changes in the healthcare industry. While we may elect to update forward-looking statements at some point in the future, we specifically disclaim any obligation to do so, even if our estimates change and, therefore, you should not rely on these forward-looking statements as representing our views as of any date subsequent to to _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Wed Nov 12 15:04:04 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Wed Nov 12 15:04:32 2008 Subject: [Histonet] RA Lamb has been sold to the evil empire In-Reply-To: <003d01c94508$c5aec530$095a5b82@vet.upenn.edu> References: <530032.77007.qm@web44916.mail.sp1.yahoo.com><000301c94507$c9d3fa50$d00f7ca5@lurie.northwestern.edu> <003d01c94508$c5aec530$095a5b82@vet.upenn.edu> Message-ID: <82E0939934624F4184F519DCE1A0FDEB@Ford> Or... "If we don't have it... YOU DON'T NEED IT!" (think about it...) ;-) Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Wednesday, November 12, 2008 2:54 PM To: 'Bernice Frederick'; 'Jack Cates'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RA Lamb has been sold to the evil empire It may become "Buy here or get nothing" Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, November 12, 2008 3:47 PM To: 'Jack Cates'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RA Lamb has been sold to the evil empire I keep telling everyone it's going to be "histosupplies are us" one of these days!!! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Cates Sent: Wednesday, November 12, 2008 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RA Lamb has been sold to the evil empire I am very sorry to see this happen again to a great company who bent over backwards to assist customers.? I hope ThemoShandonFisherRichardRaymond (to be continued) will start to keep the parts that made Richard Allen, Raymond A. Lamb and others great to work with I am also very sorry to see Tonia and Jerry go. Tuesday, Nov. 11, 2008 Thermo Fisher Scientific Strengthens Anatomical Pathology Portfolio with Acquisition of Raymond A. Lamb WALTHAM, Mass. ? Thermo Fisher Scientific Inc., the world leader in serving science, today announced it has acquired Raymond A. Lamb Ltd., a manufacturer of histology and anatomical pathology products based in Eastbourne, U.K., near London. In business since 1964, Raymond A. Lamb has grown to become a global provider of products for the pathology laboratory. Recently, its focus has been on systems that automate the labeling and tracking of slides and cassettes that carry patient tissue samples, reducing the possibility of errors during their processing, storage and retrieval. The company has had a longstanding relationship with the anatomical pathology business of Thermo Fisher as a supplier of labeling products for cassettes and glass slides, as well as other anatomical pathology equipment. "Our anatomical pathology customers - in both clinical and research markets - are demanding better solutions for tracking the increasing number of samples that they need to process and diagnose," said Marijn E. Dekkers, president and chief executive officer of Thermo Fisher Scientific. "This acquisition brings new-generation systems that can be integrated with our existing portfolio of anatomical pathology equipment and consumables to create a more efficient, and reliable, workflow." Raymond A. Lamb had revenues of approximately $9 million in 2007, and will be integrated into Thermo Fisher's Analytical Technologies Segment. About Thermo Fisher Scientific Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With annual revenues of $10 billion, we have more than 30,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. Thermo Scientific offers customers a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com. Advertisement The following constitutes a "Safe Harbor" statement under the Private Securities Litigation Reform Act of 1995: This press release contains forward-looking statements that involve a number of risks and uncertainties. Important factors that could cause actual results to differ materially from those indicated by such forward-looking statements are set forth in the company's Quarterly Report on Form 10-Q for the quarter ended September 27, 2008, under the caption "Risk Factors," which is on file with the Securities and Exchange Commission (SEC) and available in the "Investors" section of our Website under the heading "SEC Filings." Important factors that could cause actual results to differ materially from those indicated by forward-looking statements include risks and uncertainties relating to: competition and its effect on pricing, spending, third-party relationships and revenues; the need to develop new products and adapt to significant technological change; implementation of strategies for improving internal growth; general worldwide economic conditions and related uncertainties; use and protection of intellectual property; dependence on customers' capital spending policies and government funding policies; realization of potential future savings from new productivity initiatives; the effect of changes in governmental regulations; the effect of exchange rate fluctuations on international operations; the effect of laws and regulations governing government contracts; the effect of competing with certain of our customers and suppliers; and the effect of rapid changes in the healthcare industry. While we may elect to update forward-looking statements at some point in the future, we specifically disclaim any obligation to do so, even if our estimates change and, therefore, you should not rely on these forward-looking statements as representing our views as of any date subsequent to to _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHargrove <@t> urhcs.org Wed Nov 12 15:09:44 2008 From: SHargrove <@t> urhcs.org (SHargrove@urhcs.org) Date: Wed Nov 12 15:10:02 2008 Subject: [Histonet] Susie Hargrove is out of the office. Message-ID: I will be out of the office starting 11/12/2008 and will not return until 11/17/2008. I will respond to your message when I return. If immediate assistance is needed please call 3198. From pmarcum <@t> vet.upenn.edu Wed Nov 12 15:13:05 2008 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Wed Nov 12 15:13:10 2008 Subject: [Histonet] RA Lamb has been sold to the evil empire In-Reply-To: <82E0939934624F4184F519DCE1A0FDEB@Ford> References: <530032.77007.qm@web44916.mail.sp1.yahoo.com><000301c94507$c9d3fa50$d00f7ca5@lurie.northwestern.edu> <003d01c94508$c5aec530$095a5b82@vet.upenn.edu> <82E0939934624F4184F519DCE1A0FDEB@Ford> Message-ID: <003e01c9450b$7487bf60$095a5b82@vet.upenn.edu> Sounds right. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: Ford Royer [mailto:froyer@bitstream.net] Sent: Wednesday, November 12, 2008 4:04 PM To: 'Pamela Marcum'; 'Bernice Frederick'; 'Jack Cates'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RA Lamb has been sold to the evil empire Or... "If we don't have it... YOU DON'T NEED IT!" (think about it...) ;-) Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Wednesday, November 12, 2008 2:54 PM To: 'Bernice Frederick'; 'Jack Cates'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RA Lamb has been sold to the evil empire It may become "Buy here or get nothing" Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, November 12, 2008 3:47 PM To: 'Jack Cates'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RA Lamb has been sold to the evil empire I keep telling everyone it's going to be "histosupplies are us" one of these days!!! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Cates Sent: Wednesday, November 12, 2008 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RA Lamb has been sold to the evil empire I am very sorry to see this happen again to a great company who bent over backwards to assist customers.? I hope ThemoShandonFisherRichardRaymond (to be continued) will start to keep the parts that made Richard Allen, Raymond A. Lamb and others great to work with I am also very sorry to see Tonia and Jerry go. Tuesday, Nov. 11, 2008 Thermo Fisher Scientific Strengthens Anatomical Pathology Portfolio with Acquisition of Raymond A. Lamb WALTHAM, Mass. ? Thermo Fisher Scientific Inc., the world leader in serving science, today announced it has acquired Raymond A. Lamb Ltd., a manufacturer of histology and anatomical pathology products based in Eastbourne, U.K., near London. In business since 1964, Raymond A. Lamb has grown to become a global provider of products for the pathology laboratory. Recently, its focus has been on systems that automate the labeling and tracking of slides and cassettes that carry patient tissue samples, reducing the possibility of errors during their processing, storage and retrieval. The company has had a longstanding relationship with the anatomical pathology business of Thermo Fisher as a supplier of labeling products for cassettes and glass slides, as well as other anatomical pathology equipment. "Our anatomical pathology customers - in both clinical and research markets - are demanding better solutions for tracking the increasing number of samples that they need to process and diagnose," said Marijn E. Dekkers, president and chief executive officer of Thermo Fisher Scientific. "This acquisition brings new-generation systems that can be integrated with our existing portfolio of anatomical pathology equipment and consumables to create a more efficient, and reliable, workflow." Raymond A. Lamb had revenues of approximately $9 million in 2007, and will be integrated into Thermo Fisher's Analytical Technologies Segment. About Thermo Fisher Scientific Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With annual revenues of $10 billion, we have more than 30,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. Thermo Scientific offers customers a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com. Advertisement The following constitutes a "Safe Harbor" statement under the Private Securities Litigation Reform Act of 1995: This press release contains forward-looking statements that involve a number of risks and uncertainties. Important factors that could cause actual results to differ materially from those indicated by such forward-looking statements are set forth in the company's Quarterly Report on Form 10-Q for the quarter ended September 27, 2008, under the caption "Risk Factors," which is on file with the Securities and Exchange Commission (SEC) and available in the "Investors" section of our Website under the heading "SEC Filings." Important factors that could cause actual results to differ materially from those indicated by forward-looking statements include risks and uncertainties relating to: competition and its effect on pricing, spending, third-party relationships and revenues; the need to develop new products and adapt to significant technological change; implementation of strategies for improving internal growth; general worldwide economic conditions and related uncertainties; use and protection of intellectual property; dependence on customers' capital spending policies and government funding policies; realization of potential future savings from new productivity initiatives; the effect of changes in governmental regulations; the effect of exchange rate fluctuations on international operations; the effect of laws and regulations governing government contracts; the effect of competing with certain of our customers and suppliers; and the effect of rapid changes in the healthcare industry. While we may elect to update forward-looking statements at some point in the future, we specifically disclaim any obligation to do so, even if our estimates change and, therefore, you should not rely on these forward-looking statements as representing our views as of any date subsequent to to _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Nov 12 16:17:08 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Nov 12 16:17:29 2008 Subject: [Histonet] RA Lamb has been sold to the evil empire References: <000301c94507$c9d3fa50$d00f7ca5@lurie.northwestern.edu> Message-ID: <0F37C04FB43E49238421D0EF57F72C54@yourxhtr8hvc4p> great. Soon there will be only one booth at the states and the NSH conventions ----- Original Message ----- From: "Bernice Frederick" To: "'Jack Cates'" ; Sent: Wednesday, November 12, 2008 2:46 PM Subject: RE: [Histonet] RA Lamb has been sold to the evil empire I keep telling everyone it's going to be "histosupplies are us" one of these days!!! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Cates Sent: Wednesday, November 12, 2008 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RA Lamb has been sold to the evil empire I am very sorry to see this happen again to a great company who bent over backwards to assist customers. I hope ThemoShandonFisherRichardRaymond (to be continued) will start to keep the parts that made Richard Allen, Raymond A. Lamb and others great to work with I am also very sorry to see Tonia and Jerry go. Tuesday, Nov. 11, 2008 Thermo Fisher Scientific Strengthens Anatomical Pathology Portfolio with Acquisition of Raymond A. Lamb WALTHAM, Mass. - Thermo Fisher Scientific Inc., the world leader in serving science, today announced it has acquired Raymond A. Lamb Ltd., a manufacturer of histology and anatomical pathology products based in Eastbourne, U.K., near London. In business since 1964, Raymond A. Lamb has grown to become a global provider of products for the pathology laboratory. Recently, its focus has been on systems that automate the labeling and tracking of slides and cassettes that carry patient tissue samples, reducing the possibility of errors during their processing, storage and retrieval. The company has had a longstanding relationship with the anatomical pathology business of Thermo Fisher as a supplier of labeling products for cassettes and glass slides, as well as other anatomical pathology equipment. "Our anatomical pathology customers - in both clinical and research markets - are demanding better solutions for tracking the increasing number of samples that they need to process and diagnose," said Marijn E. Dekkers, president and chief executive officer of Thermo Fisher Scientific. "This acquisition brings new-generation systems that can be integrated with our existing portfolio of anatomical pathology equipment and consumables to create a more efficient, and reliable, workflow." Raymond A. Lamb had revenues of approximately $9 million in 2007, and will be integrated into Thermo Fisher's Analytical Technologies Segment. About Thermo Fisher Scientific Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With annual revenues of $10 billion, we have more than 30,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. Thermo Scientific offers customers a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com. Advertisement The following constitutes a "Safe Harbor" statement under the Private Securities Litigation Reform Act of 1995: This press release contains forward-looking statements that involve a number of risks and uncertainties. Important factors that could cause actual results to differ materially from those indicated by such forward-looking statements are set forth in the company's Quarterly Report on Form 10-Q for the quarter ended September 27, 2008, under the caption "Risk Factors," which is on file with the Securities and Exchange Commission (SEC) and available in the "Investors" section of our Website under the heading "SEC Filings." Important factors that could cause actual results to differ materially from those indicated by forward-looking statements include risks and uncertainties relating to: competition and its effect on pricing, spending, third-party relationships and revenues; the need to develop new products and adapt to significant technological change; implementation of strategies for improving internal growth; general worldwide economic conditions and related uncertainties; use and protection of intellectual property; dependence on customers' capital spending policies and government funding policies; realization of potential future savings from new productivity initiatives; the effect of changes in governmental regulations; the effect of exchange rate fluctuations on international operations; the effect of laws and regulations governing government contracts; the effect of competing with certain of our customers and suppliers; and the effect of rapid changes in the healthcare industry. While we may elect to update forward-looking statements at some point in the future, we specifically disclaim any obligation to do so, even if our estimates change and, therefore, you should not rely on these forward-looking statements as representing our views as of any date subsequent to to _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Nov 12 16:33:08 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Nov 12 16:33:44 2008 Subject: [Histonet] RA Lamb has been sold to the evil empire References: <000301c94507$c9d3fa50$d00f7ca5@lurie.northwestern.edu> <0F37C04FB43E49238421D0EF57F72C54@yourxhtr8hvc4p> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A73DF727@LTA3VS011.ees.hhs.gov> Crap. So much for the hospitalities. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Wed 11/12/2008 5:17 PM To: Bernice Frederick; 'Jack Cates'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RA Lamb has been sold to the evil empire great. Soon there will be only one booth at the states and the NSH conventions ----- Original Message ----- From: "Bernice Frederick" To: "'Jack Cates'" ; Sent: Wednesday, November 12, 2008 2:46 PM Subject: RE: [Histonet] RA Lamb has been sold to the evil empire I keep telling everyone it's going to be "histosupplies are us" one of these days!!! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jack Cates Sent: Wednesday, November 12, 2008 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RA Lamb has been sold to the evil empire I am very sorry to see this happen again to a great company who bent over backwards to assist customers. I hope ThemoShandonFisherRichardRaymond (to be continued) will start to keep the parts that made Richard Allen, Raymond A. Lamb and others great to work with I am also very sorry to see Tonia and Jerry go. Tuesday, Nov. 11, 2008 Thermo Fisher Scientific Strengthens Anatomical Pathology Portfolio with Acquisition of Raymond A. Lamb WALTHAM, Mass. - Thermo Fisher Scientific Inc., the world leader in serving science, today announced it has acquired Raymond A. Lamb Ltd., a manufacturer of histology and anatomical pathology products based in Eastbourne, U.K., near London. In business since 1964, Raymond A. Lamb has grown to become a global provider of products for the pathology laboratory. Recently, its focus has been on systems that automate the labeling and tracking of slides and cassettes that carry patient tissue samples, reducing the possibility of errors during their processing, storage and retrieval. The company has had a longstanding relationship with the anatomical pathology business of Thermo Fisher as a supplier of labeling products for cassettes and glass slides, as well as other anatomical pathology equipment. "Our anatomical pathology customers - in both clinical and research markets - are demanding better solutions for tracking the increasing number of samples that they need to process and diagnose," said Marijn E. Dekkers, president and chief executive officer of Thermo Fisher Scientific. "This acquisition brings new-generation systems that can be integrated with our existing portfolio of anatomical pathology equipment and consumables to create a more efficient, and reliable, workflow." Raymond A. Lamb had revenues of approximately $9 million in 2007, and will be integrated into Thermo Fisher's Analytical Technologies Segment. About Thermo Fisher Scientific Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With annual revenues of $10 billion, we have more than 30,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. Thermo Scientific offers customers a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com. Advertisement The following constitutes a "Safe Harbor" statement under the Private Securities Litigation Reform Act of 1995: This press release contains forward-looking statements that involve a number of risks and uncertainties. Important factors that could cause actual results to differ materially from those indicated by such forward-looking statements are set forth in the company's Quarterly Report on Form 10-Q for the quarter ended September 27, 2008, under the caption "Risk Factors," which is on file with the Securities and Exchange Commission (SEC) and available in the "Investors" section of our Website under the heading "SEC Filings." Important factors that could cause actual results to differ materially from those indicated by forward-looking statements include risks and uncertainties relating to: competition and its effect on pricing, spending, third-party relationships and revenues; the need to develop new products and adapt to significant technological change; implementation of strategies for improving internal growth; general worldwide economic conditions and related uncertainties; use and protection of intellectual property; dependence on customers' capital spending policies and government funding policies; realization of potential future savings from new productivity initiatives; the effect of changes in governmental regulations; the effect of exchange rate fluctuations on international operations; the effect of laws and regulations governing government contracts; the effect of competing with certain of our customers and suppliers; and the effect of rapid changes in the healthcare industry. While we may elect to update forward-looking statements at some point in the future, we specifically disclaim any obligation to do so, even if our estimates change and, therefore, you should not rely on these forward-looking statements as representing our views as of any date subsequent to to _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Wed Nov 12 17:15:41 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Nov 12 17:15:57 2008 Subject: [Histonet] kappa/lambda and.. In-Reply-To: <01F4C23B139344A58D76932541547D8D@dielangs.at> Message-ID: Yep, Extensive NBF fixation tends to rinse out the extra-cellular immunoglobulins and so decreases its contribution to the background staining. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Thursday, 13 November 2008 5:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] kappa/lambda and.. Thank you for your answers. An additional question: Can someone confirm, that underfixed tissue shows false positive kappa and lambdastaining? Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Mittwoch, 12. November 2008 16:45 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] experiences with kappa/lambda Hi listmembers, Please tell me about your experiences with the IHC-demonstration of kappa and lambda on FFPE-sections. We have some troubles about inconsistend staining and therefore false results about the monoclonality of tumors. Bye Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From CIngles <@t> uwhealth.org Wed Nov 12 17:31:01 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Wed Nov 12 17:32:03 2008 Subject: [Histonet] RA Lamb has been sold to the evil empire References: <000301c94507$c9d3fa50$d00f7ca5@lurie.northwestern.edu> <0F37C04FB43E49238421D0EF57F72C54@yourxhtr8hvc4p> Message-ID: <08A0A863637F1349BBFD83A96B27A50A1201AE@uwhis-xchng3.uwhis.hosp.wisc.edu> Yea, but what a booth! Think of the size. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Wed 11/12/2008 4:17 PM To: Bernice Frederick; 'Jack Cates'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RA Lamb has been sold to the evil empire great. Soon there will be only one booth at the states and the NSH conventions From abright <@t> brightinstruments.com Thu Nov 13 05:08:24 2008 From: abright <@t> brightinstruments.com (Alan Bright) Date: Thu Nov 13 05:41:26 2008 Subject: [Histonet] Plant Tissue Processing In-Reply-To: References: Message-ID: <3EFBB875DEE1994FB040A0B099F3AC8A0ACD39@BRIGHT-SBS.Bright.local> Dear Peggy, I would have thought cryo sectioning would give much better results. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peggy Bisher Sent: 12 November 2008 17:02 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Plant Tissue Processing I have a professor here who would like me to make 10-15 micron thick sections of lawn grass. I thought the best way to do this would be paraffin embedding. Does anybody have any suggestions as to the time/types for my paraffin tissue processor? I am not very familiar working with plant material. Thank you very much, Maggie Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher@princeton.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Thu Nov 13 06:24:28 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Nov 13 06:24:33 2008 Subject: [Histonet] Looking for Dave Lockhart and DeeAnn Paterson Message-ID: <4f016b690811130424y7345deah3059e33e94325ac8@mail.gmail.com> Hi! If anyone know how I might get in contact with either of these people, please let me know. Also if either of you are out there, would you please drop me a line? I would very much appreciate it. Thanks Vikki Baker From srogers1 <@t> ameripath.com Thu Nov 13 08:10:40 2008 From: srogers1 <@t> ameripath.com (Rogers, Steve) Date: Thu Nov 13 08:16:47 2008 Subject: [Histonet] (no subject) Message-ID: Hello I am looking for anyone that can help me find out about nail Cultures. Steve R. Rogers Jr. (HT) ASCP Histology Laboratory Manager DermPath Diagnostics/AmeriPath Pittsburgh 5001 Centre Ave Third Floor Pittsburgh, PA 15213 412-682-3083 From lyork <@t> kwbpathology.com Thu Nov 13 08:29:22 2008 From: lyork <@t> kwbpathology.com (LYork) Date: Thu Nov 13 08:29:41 2008 Subject: [Histonet] Florida Job Message-ID: <000701c9459c$3f60e820$8e01a8c0@KWBPA.local> Private laboratory in Tallahassee, Florida looking for HT (ASCP) to fill 3rd shift position. Hours are Monday-Thursday 10p-8a. Excellent salary and benefit package as well as 20% shift differential. Please contact lyork@kwbpathology.com for more information. Must be Florida licensed or eligible. From ree3 <@t> leicester.ac.uk Thu Nov 13 09:18:12 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Nov 13 09:18:18 2008 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <7722595275A4DD4FA225B92CDBF174A1744E1B45E0@EXC-MBX3.cfs.le.ac.uk> The subject of Nail Cultures is very close to my heart, having studied the ancient civilisations in which the nail replaced the more degradable wooden rivet, I can let you have a copy of my treatise "Nailed On" which details the rise and demise of the Nail Cultures before they were overtaken in the evolutionary race by the more dynamic Screw Civilisations beautifully described in my illustrated and comprehensive work "Screwu". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rogers, Steve Sent: 13 November 2008 14:11 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello I am looking for anyone that can help me find out about nail Cultures. Steve R. Rogers Jr. (HT) ASCP Histology Laboratory Manager DermPath Diagnostics/AmeriPath Pittsburgh 5001 Centre Ave Third Floor Pittsburgh, PA 15213 412-682-3083 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Nov 13 09:23:13 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Nov 13 09:23:40 2008 Subject: AW: [Histonet] kappa/lambda and.. In-Reply-To: References: <01F4C23B139344A58D76932541547D8D@dielangs.at> Message-ID: <86C7389C20744B7087CEA552A0324716@dielangs.at> What duration would you see as "extensive" fixation? 18-24 hours? Or beyond? Regards Gudrun -----Urspr?ngliche Nachricht----- Von: Tony Henwood [mailto:AnthonyH@chw.edu.au] Gesendet: Donnerstag, 13. November 2008 00:16 An: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] kappa/lambda and.. Yep, Extensive NBF fixation tends to rinse out the extra-cellular immunoglobulins and so decreases its contribution to the background staining. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Thursday, 13 November 2008 5:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] kappa/lambda and.. Thank you for your answers. An additional question: Can someone confirm, that underfixed tissue shows false positive kappa and lambdastaining? Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Mittwoch, 12. November 2008 16:45 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] experiences with kappa/lambda Hi listmembers, Please tell me about your experiences with the IHC-demonstration of kappa and lambda on FFPE-sections. We have some troubles about inconsistend staining and therefore false results about the monoclonality of tumors. Bye Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From gu.lang <@t> gmx.at Thu Nov 13 09:29:22 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Nov 13 09:29:41 2008 Subject: [Histonet] kappa/lambda CISH Message-ID: <40E4F33EAB7C4188B43F6D1FA5FB6F4E@dielangs.at> Again a question about this issue: Has anybody experience with CISH on acid decalcified bonemarrow biopsies? We use formic acid ("Calrite") for 8 hours after NBF-fixation. In my opinion the acid would destroy DNA and RNA. And therefore a hybridization cannot be successful or at least not reliable. Gudrun Lang Histolab, Akh Linz, Austria From cristiana.soldani <@t> humanitas.it Thu Nov 13 09:17:17 2008 From: cristiana.soldani <@t> humanitas.it (SOLDANI Cristiana ICH) Date: Thu Nov 13 10:02:09 2008 Subject: [Histonet] alkaline phosphatase Message-ID: <8D4E4E312BEF164997299EE5B0E12B7C023C364E@TEHWEX11.techosp.it> I would like to know if someone used alkaline phosphatase on cryostatic tissue section to block the tyrosine. I have alkaline phosphatase from shrimp (sigma) but I don't know how many units I have to use, the temperature, the time.... Many thanks From ian.montgomery <@t> bio.gla.ac.uk Thu Nov 13 10:24:45 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Nov 13 10:27:35 2008 Subject: [Histonet] Buffer. Message-ID: What is the recommended EM buffer for sea water fish? I had thought of using sea water itself but would cacodylate or one of the phosphates be better and at what molarity? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From froyer <@t> bitstream.net Thu Nov 13 10:50:53 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Nov 13 10:51:39 2008 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <2266871DC7484FD7B6A250C8FD0EA8DB@Ford> Are you asking about how to culture & identify fungi that infect nails, or is this a tissue culture question? Can you be more specific? Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 Web: http://www.minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rogers, Steve Sent: Thursday, November 13, 2008 8:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello I am looking for anyone that can help me find out about nail Cultures. Steve R. Rogers Jr. (HT) ASCP Histology Laboratory Manager DermPath Diagnostics/AmeriPath Pittsburgh 5001 Centre Ave Third Floor Pittsburgh, PA 15213 412-682-3083 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Thu Nov 13 12:23:46 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Nov 13 12:23:51 2008 Subject: [Histonet] MegaSupplyCompanyInc Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6597@nmdamailsvr.nmda.ad.nmsu.edu> Didn't the government BREAK UP AT&T years ago for this same thing??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From jqb7 <@t> cdc.gov Thu Nov 13 12:26:15 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Nov 13 12:26:31 2008 Subject: [Histonet] fibrin Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C4F6@LTA3VS011.ees.hhs.gov> Happy Thursday everyone! I would like to know what special stain is being used for fibrin demonstration. Thanks in advance for your help! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From tbraud <@t> holyredeemer.com Thu Nov 13 12:27:49 2008 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Nov 13 12:27:53 2008 Subject: [Histonet] RE: Histonet Digest, Nail Cultures In-Reply-To: <0a07e44f0000ad6f@HolyRedeemer.com> Message-ID: OMG! This was the funniest response that I've read in ages. All of the techs were running into my office to see what I was cackling about. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 Message: 8 Date: Thu, 13 Nov 2008 15:18:12 +0000 From: "Edwards, R.E." Subject: RE: [Histonet] (no subject) To: "'Rogers, Steve'" , The subject of Nail Cultures is very close to my heart, having studied the ancient civilisations in which the nail replaced the more degradable wooden rivet, I can let you have a copy of my treatise "Nailed On" which details the rise and demise of the Nail Cultures before they were overtaken in the evolutionary race by the more dynamic Screw Civilisations beautifully described in my illustrated and comprehensive work "Screwu". --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From histonetalias <@t> gmail.com Thu Nov 13 12:39:04 2008 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Thu Nov 13 12:39:08 2008 Subject: [Histonet] fibrin In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A70208C4F6@LTA3VS011.ees.hhs.gov> References: <1CE1847DFEA0A647B1CCDE4108EA60A70208C4F6@LTA3VS011.ees.hhs.gov> Message-ID: <4b6c85510811131039m5d114c3ard0c69ea1e63c623a@mail.gmail.com> Try the Martius scarlet blue (MSB) or PTAH. On Thu, Nov 13, 2008 at 1:26 PM, Bartlett, Jeanine (CDC/CCID/NCZVED) < jqb7@cdc.gov> wrote: > Happy Thursday everyone! > > I would like to know what special stain is being used for fibrin > demonstration. > > Thanks in advance for your help! > > Jeanine Bartlett, BS, HT(ASCP)QIHC > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA 30333 > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- The Unknown HT(ASCP) From pumla.pamla <@t> gmail.com Thu Nov 13 13:09:07 2008 From: pumla.pamla <@t> gmail.com (Pumla Pamla-Gutter) Date: Thu Nov 13 13:09:12 2008 Subject: [Histonet] Reprocessing Hematoxylin&Eosin tongue slides. Message-ID: Hello, Could anyone help me with a protocol for re staining H&E formalin-fixed tissue sections? I have some H&E stained tongue slides that are too weak and would like to reprocess them. Could I just soak them in xylene, rehydrate and then place in hematoxylin? Any ideas what that does to the counterstain with Eosin? By the way: I used Gill's Hematoxylin (water+ethylene gycol+hematoxylin+sodium iodate+aluminum sulfate+acetic acid) Thank you, -- Pumla Pamla-Gutter Research Assistant I Ohio State University College of Dentistry 305W. 12th Avenue Columbus, OH, 43232 Office: (614) 292-4046 Fax: (614) 247-6945 Email: pamla-gutter.1@osu.edu From tora.bardal <@t> bio.ntnu.no Thu Nov 13 13:34:59 2008 From: tora.bardal <@t> bio.ntnu.no (Tora Bardal) Date: Thu Nov 13 13:35:07 2008 Subject: [Histonet] Buffer. In-Reply-To: References: Message-ID: <491C8163.2020904@bio.ntnu.no> Hello Ian, We use a mixture of 2.5% glutardialdehyde, 2.5% paraformaldehyde and 0.5% sucrose in 0.08 M cacodylic buff?er, pH 7.4 to 7.6 Galloway, Trina Falck; Kj?rsvik, Elin; Kryvi, Harald. Effect of temperature on viability and axial muscle development in embryos and yolk sac larvae of the Northeast Arctic cod (*Gadus morhua*).. /Marine Biology/ 1998 ;Volum 132.(4) s. 559-567 Tora NTNU Center of Fisheries and Aquaculture Ian Montgomery wrote: > What is the recommended EM buffer for sea water fish? I had >thought of using sea water itself but would cacodylate or one of the >phosphates be better and at what molarity? > >Ian. > > > > From froyer <@t> bitstream.net Thu Nov 13 13:41:52 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Nov 13 13:42:21 2008 Subject: [Histonet] (no subject) In-Reply-To: References: <2266871DC7484FD7B6A250C8FD0EA8DB@Ford> Message-ID: Once again... What are you trying to culture from nails? Bacteria in nails? Fungus in nails? Tissue culture of nails? Equipment requirements will differ depending on what you are trying to do. Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 Web: http://www.minnesotamedical.com -----Original Message----- From: Rogers, Steve [mailto:srogers1@ameripath.com] Sent: Thursday, November 13, 2008 10:59 AM To: Ford Royer Subject: RE: [Histonet] (no subject) I need information how equipment needed for nail cultures. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Thursday, November 13, 2008 11:51 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) Are you asking about how to culture & identify fungi that infect nails, or is this a tissue culture question? Can you be more specific? Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 Web: http://www.minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rogers, Steve Sent: Thursday, November 13, 2008 8:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello I am looking for anyone that can help me find out about nail Cultures. Steve R. Rogers Jr. (HT) ASCP Histology Laboratory Manager DermPath Diagnostics/AmeriPath Pittsburgh 5001 Centre Ave Third Floor Pittsburgh, PA 15213 412-682-3083 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Thu Nov 13 13:59:35 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Nov 13 13:59:47 2008 Subject: [Histonet] alkaline phosphatase In-Reply-To: <8D4E4E312BEF164997299EE5B0E12B7C023C364E@TEHWEX11.techosp.it> References: <8D4E4E312BEF164997299EE5B0E12B7C023C364E@TEHWEX11.techosp.it> Message-ID: Alkaline phosphatases are enzymes that catalyse the hydrolysis of phosph (formed by acti the phenolic side-ch blocking reactions for are:
    Acylation, with acetic anhydride of benzoyl chloride
    iodine
    Nitrati tetranitromethane.
None of these reactions are speci tyrosine, but they will prevent histochemical reactions that g enerate coloured products with that amino acid, such as the Millon tes UWO
=

---Original Message-- Cristiana ICH <cristiana.soldani@hu manitas.it>
Date: Thursday, November 13, 2008 11: phosphatase
To: hi stonet@lists.utsouthwestern.edu

> I would li
> cryo tyrosine.
>
& phosphatase from shrimp (sigma) but I don't know how
> many units I have to use, the temperature, the thanks
& _________________ _______________________ 5F list
> Histonet@lists.utsouthwestern.edu
> http://lists. From AnthonyH <@t> chw.edu.au Thu Nov 13 14:35:45 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Nov 13 14:35:59 2008 Subject: [Histonet] kappa/lambda and.. In-Reply-To: <86C7389C20744B7087CEA552A0324716@dielangs.at> Message-ID: You are probably correct. Remember it would only be one of the variables that affects this localisation. A similar phenomenon occurs with yolk sac tumours stained for alpha fetoprotein. Short fixation often give a heavy background where as if the tissue is allowed to "stew" in formalin for several weeks a lot of this staining disappears. We found this with control blocks that were prepared several months after the initial diagnostic stain. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Gudrun Lang [mailto:gu.lang@gmx.at] Sent: Friday, 14 November 2008 2:23 AM To: Tony Henwood Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] kappa/lambda and.. What duration would you see as "extensive" fixation? 18-24 hours? Or beyond? Regards Gudrun -----Urspr?ngliche Nachricht----- Von: Tony Henwood [mailto:AnthonyH@chw.edu.au] Gesendet: Donnerstag, 13. November 2008 00:16 An: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] kappa/lambda and.. Yep, Extensive NBF fixation tends to rinse out the extra-cellular immunoglobulins and so decreases its contribution to the background staining. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Thursday, 13 November 2008 5:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] kappa/lambda and.. Thank you for your answers. An additional question: Can someone confirm, that underfixed tissue shows false positive kappa and lambdastaining? Bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Mittwoch, 12. November 2008 16:45 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] experiences with kappa/lambda Hi listmembers, Please tell me about your experiences with the IHC-demonstration of kappa and lambda on FFPE-sections. We have some troubles about inconsistend staining and therefore false results about the monoclonality of tumors. Bye Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From jkiernan <@t> uwo.ca Thu Nov 13 14:36:50 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Nov 13 14:36:55 2008 Subject: [Histonet] Plant Tissue Processing Message-ID: Soft plant tissues are easily damaged before fixation. Cut (slice, not chop) with a very sharp razor blade, not scissors. The high water content can lead to collapse during dehydration, so this needs to be done gradually, especially through concentrations of alcohols greater than about 50%. A formalin-acetic-alcohol fixative with 50% alcohol is a good fixative for plants. I have used Davidson's fixative, which contains somewhat less alcohol, for stems, flowers and leaves, with good results. I've never tried making sections of grass. There are several solvents and techniques for slowly and gently dehydrating and clearing plant and other "delicate" watery tissues. I strongly recommend "Botanical Microtechnique and Cytochemustry" by GP Berlyn & JP Miksche. Iowa State Univ Press, Ames IA (1976) ISBN 813802202. This 326-page hardcover book may still be available new, from the publisher, quite inexpensively. It gives very detailed descriptions of collection, fixation, processing, embedding and cutting sections (paraffin and plastic). A machine processing schedule for chunks of animal organs probably would be too harsh for non-woody plant material. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Alan Bright Date: Thursday, November 13, 2008 6:43 Subject: RE: [Histonet] Plant Tissue Processing To: Peggy Bisher , Histonet@lists.utsouthwestern.edu > Dear Peggy, > > I would have thought cryo sectioning would give much better > results. > > > Best Regards > > Alan Bright > > Bright Instrument Co.Ltd. > St Margaret's Way > Huntingdon > Cambridgeshire > PE29 6EU > England > > Tel No:+44 (0)1480 454528 > Fax No:+44 (0)1480 456031 > Email: abright@brightinstruments.com > Web Site: www.brightinstruments.com > Skype: dazzle0 > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peggy > Bisher > Sent: 12 November 2008 17:02 > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Plant Tissue Processing > > I have a professor here who would like me to make 10-15 micron thick > sections of lawn grass. I thought the best way to do this would be > paraffin > embedding. Does anybody have any suggestions as to the > time/types for my > paraffin tissue processor? I am not very familiar working with plant > material. > > Thank you very much, Maggie > > > Margaret E. Bisher > Electron Microscopy & Histology Core Facility Manager > Department of Molecular Biology > Princeton University > Moffett Laboratory, Room 113 > Princeton, New Jersey > Office: (609) 258-7026 > Fax: (609) 258-8468 > mbisher@princeton.edu > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aevans <@t> wellspan.org Thu Nov 13 14:46:25 2008 From: aevans <@t> wellspan.org (Evans, Andria B.) Date: Thu Nov 13 14:54:30 2008 Subject: [Histonet] GI Biopsies Message-ID: We are having difficulty with our GI biopsies having a lot of chatter on the H & E. I think somehow they are getting over processed, but not sure.... has anyone had this problem or could suggest what might be causing this and/or solutions. Thank you! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ From sbreeden <@t> nmda.nmsu.edu Thu Nov 13 14:58:57 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Nov 13 14:59:02 2008 Subject: [Histonet] Website Hosting by LabVision? Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E65A1@nmdamailsvr.nmda.ad.nmsu.edu> Would the person (LabVision?) who manages website hosting for State Histology Societies please contact me? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From juditw <@t> u.washington.edu Thu Nov 13 15:13:21 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Thu Nov 13 15:13:35 2008 Subject: [Histonet] Buffer. In-Reply-To: Message-ID: Hi Ian - I just happen to have a copy of "Fixation of Fish Tissue" by Fournie, Krol, Hawkins- CH 34, Academic Press, 2000. I have the excerpt so I do not know the title of the book. I quote them- " The osmolality range for mammalian tissues are routinely used. For fish, glutaraldehyde fixatives in cacodylate buffer wihch fall with in the osmolality range for mammalian tissues are routinely used. Karnovsky's fixative (about 2000 mosm) also is often used for marine specimens. The additon of non-electroylytes (sucrose, glucose, dextran) and electroylytes (CaCL2 and NaCl) stabilize osmolarity and decrease cell swelling. Recommended formula: 2.5% glutaraldehyde in 0.1M Sodium Cacodylate buffer with 0.6 M sucrose O.2M sodium cacoduylate buffer, pH7.4 .........250.0 ml glutaraldehyde, 25% ........................... 50.0 ml sucrose.........................................100 gm distilled water .............................500 gm Hope this is helpful. I have good friends who do a lot of fish histology at Gulf Coast Research Laboratory, Univ. of Southern Miss. Ocean Springs, MS. If you have further questions- contact Nancy-Brown Peterson there. Judy On Thu, 13 Nov 2008, Ian Montgomery wrote: > What is the recommended EM buffer for sea water fish? I had > thought of using sea water itself but would cacodylate or one of the > phosphates be better and at what molarity? > > Ian. > > > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From mickie25 <@t> netzero.net Thu Nov 13 15:50:43 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Thu Nov 13 15:51:40 2008 Subject: [Histonet] GI Biopsies In-Reply-To: References: Message-ID: Dear Andria, This sounds like over-processing. If you are using a regular processing schedule it is for sure. At Sacred Heart Medical Center in Spokane we used a 2 hour 45 minute program with 15 minutes in each station. We still soaked the blocks in water before icing and cutting. Sometimes we had to soak for the second or third level. We fixed in Bouin's. Also cutting with a slower speed as the knife passed through the tissue helped. Hope this helps Best Regards, ? Mickie ? Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC ? & Mohs Lab Staffing & Mohs Suite Mohs Reporting Software 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX?? 509-624-3926 Web: www.mohshistogyconsulting.com?& www.mohslabstaffing.com & www.mohssuite.com Email: mickie25@netzero.net ? DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. Thank You. ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B. Sent: Thursday, November 13, 2008 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI Biopsies We are having difficulty with our GI biopsies having a lot of chatter on the H & E. I think somehow they are getting over processed, but not sure.... has anyone had this problem or could suggest what might be causing this and/or solutions. Thank you! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.9.2/1783 - Release Date: 11/12/2008 10:01 AM From mickie25 <@t> netzero.net Thu Nov 13 15:52:22 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Thu Nov 13 15:53:01 2008 Subject: [Histonet] Reprocessing Hematoxylin&Eosin tongue slides. In-Reply-To: References: Message-ID: Yes. Best Regards, ? Mickie ? Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC ? & Mohs Lab Staffing & Mohs Suite Mohs Reporting Software 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX?? 509-624-3926 Web: www.mohshistogyconsulting.com?& www.mohslabstaffing.com & www.mohssuite.com Email: mickie25@netzero.net ? DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. Thank You. ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pumla Pamla-Gutter Sent: Thursday, November 13, 2008 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reprocessing Hematoxylin&Eosin tongue slides. Hello, Could anyone help me with a protocol for re staining H&E formalin-fixed tissue sections? I have some H&E stained tongue slides that are too weak and would like to reprocess them. Could I just soak them in xylene, rehydrate and then place in hematoxylin? Any ideas what that does to the counterstain with Eosin? By the way: I used Gill's Hematoxylin (water+ethylene gycol+hematoxylin+sodium iodate+aluminum sulfate+acetic acid) Thank you, -- Pumla Pamla-Gutter Research Assistant I Ohio State University College of Dentistry 305W. 12th Avenue Columbus, OH, 43232 Office: (614) 292-4046 Fax: (614) 247-6945 Email: pamla-gutter.1@osu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.9.2/1783 - Release Date: 11/12/2008 10:01 AM From LINDA.MARGRAF <@t> childrens.com Thu Nov 13 16:02:30 2008 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Thu Nov 13 16:09:09 2008 Subject: [Histonet] Newly blue Message-ID: <491C4F96.F783.00DA.0@childrens.com> I am posting a message for Jodie..... Hi all. I*m new to posting to this list but I*ve been observing for several years. I was wondering if anyone has heard of or tried the *Newly Blue* Hematoxylin substitute from Newcomer Supply? I saw information on it and am curious to try it since the price of Hematoxylin still seems to be thru the roof. Any feedback would be greatly appreciated. Thanks. Jodie Robertson Please consider the environment before printing this e-mail

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From renafail <@t> bellsouth.net Thu Nov 13 16:38:41 2008 From: renafail <@t> bellsouth.net (renafail@bellsouth.net) Date: Thu Nov 13 16:38:45 2008 Subject: [Histonet] fibrin In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A70208C4F6@LTA3VS011.ees.hhs.gov> Message-ID: <111320082238.16395.491CAC71000A179A0000400B22230704929B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net> The Fraser-Lendrum in the AFIP manual demonstrates fibrin Rena Fail -------------- Original message from "Bartlett, Jeanine (CDC/CCID/NCZVED)" : -------------- > Happy Thursday everyone! > > I would like to know what special stain is being used for fibrin > demonstration. > > Thanks in advance for your help! > > Jeanine Bartlett, BS, HT(ASCP)QIHC > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA 30333 > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stjosthe <@t> ualberta.ca Thu Nov 13 17:56:37 2008 From: stjosthe <@t> ualberta.ca (stjosthe@ualberta.ca) Date: Thu Nov 13 17:56:41 2008 Subject: [Histonet] Please Remove Me Message-ID: <20081113165637.86615xo2ycgwaytw@webmail.ualberta.ca> From alzcarlos <@t> hotmail.com Thu Nov 13 21:24:49 2008 From: alzcarlos <@t> hotmail.com (Carlos Hotmail) Date: Thu Nov 13 21:24:56 2008 Subject: [Histonet] Another "Pen Shell" muscle issue Message-ID: Hi all I am returning to the histonet for some help. The previous time I asked for help because I cannot stain correctly the muscle tissue of a mollusc, the "Pen Shell" Atrina maura. I returned because neither PTAH, Mallory?s, Masson?s or Milligan?s trichromes stained consitently the muscle (smooth or striated) of this evasive species. All the times I run a stain made some positives with sections of muscle from other organisms, vetebrate and invertebrates. And in all lf them the muscle looked right. All but the Pen Shell. All the times almost all or every muscle cell stained as connective tissue, with very very small sections with a positive muscle reaction. Anyone could help me to know how the tipical mammal muscle tissue stains? I think if I know how the chemistry works I could find the exact problem with my stains. All the samples are fixed in formalin 10% or Davidson, processed in ethanol series, Hemo-de (xilol substitute of limonene), paraplas plus and sectioned at 4 microns with a leica microtome. thanks (please give me an apologyze for my bad english writing) Carlos Augusto Aguilar Cruz Marine Biologist Histology Laboratory at Unidad Pichilingue Universidad Aut?noma de Baja California Sur La Paz, Baja California Sur, M?xico From llewllew <@t> shaw.ca Thu Nov 13 22:00:49 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Nov 13 22:00:58 2008 Subject: [Histonet] Another "Pen Shell" muscle issue References: Message-ID: <99C233E2CF1B4F52AB8C3CAFEA30BA05@Compaq> Try using the picro-mallory. This method differs from the usual trichromes by adding a step with small molecular weight, yellow acid dyes. Possibly, the muscle in this mollusc may stain with them. Technical details are at: http://stainsfile.info/StainsFile/stain/fibrin/picro-mallory-2.htm For a discussion on how trichrome stains work go to: http://stainsfile.info/StainsFile/theory/tri_gen.htm Bryan Llewellyn ----- Original Message ----- From: "Carlos Hotmail" To: Sent: Thursday, November 13, 2008 7:24 PM Subject: [Histonet] Another "Pen Shell" muscle issue Hi all I am returning to the histonet for some help. The previous time I asked for help because I cannot stain correctly the muscle tissue of a mollusc, the "Pen Shell" Atrina maura. I returned because neither PTAH, Mallory?s, Masson?s or Milligan?s trichromes stained consitently the muscle (smooth or striated) of this evasive species. All the times I run a stain made some positives with sections of muscle from other organisms, vetebrate and invertebrates. And in all lf them the muscle looked right. All but the Pen Shell. All the times almost all or every muscle cell stained as connective tissue, with very very small sections with a positive muscle reaction. Anyone could help me to know how the tipical mammal muscle tissue stains? I think if I know how the chemistry works I could find the exact problem with my stains. All the samples are fixed in formalin 10% or Davidson, processed in ethanol series, Hemo-de (xilol substitute of limonene), paraplas plus and sectioned at 4 microns with a leica microtome. thanks (please give me an apologyze for my bad english writing) Carlos Augusto Aguilar Cruz Marine Biologist Histology Laboratory at Unidad Pichilingue Universidad Aut?noma de Baja California Sur La Paz, Baja California Sur, M?xico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pieronelva01 <@t> bigpond.com Fri Nov 14 01:24:19 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Fri Nov 14 01:24:25 2008 Subject: [Histonet] fibrin References: <1CE1847DFEA0A647B1CCDE4108EA60A70208C4F6@LTA3VS011.ees.hhs.gov> <4b6c85510811131039m5d114c3ard0c69ea1e63c623a@mail.gmail.com> Message-ID: <8240EC72735B4A47B94AC1DD30E26D78@pentium4> I agree about the MSB stain. It's the standard fibrin stain taught to my students in Histo. And it looks great!! Piero Nelva Anatomical Pathology Monash Medical Centre Australia ----- Original Message ----- From: "Histonet Alias" To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Cc: Sent: Friday, November 14, 2008 5:39 AM Subject: Re: [Histonet] fibrin > Try the Martius scarlet blue (MSB) or PTAH. > > On Thu, Nov 13, 2008 at 1:26 PM, Bartlett, Jeanine (CDC/CCID/NCZVED) < > jqb7@cdc.gov> wrote: > >> Happy Thursday everyone! >> >> I would like to know what special stain is being used for fibrin >> demonstration. >> >> Thanks in advance for your help! >> >> Jeanine Bartlett, BS, HT(ASCP)QIHC >> Centers for Disease Control and Prevention >> Infectious Diseases Pathology Branch >> 1600 Clifton Road, MS/G-32 >> 18/SB-114 >> Atlanta, GA 30333 >> (404) 639-3590 >> jeanine.bartlett@cdc.hhs.gov >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > The Unknown HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.9.3/1786 - Release Date: 11/13/2008 6:01 PM From pieronelva01 <@t> bigpond.com Fri Nov 14 01:26:41 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Fri Nov 14 01:26:45 2008 Subject: [Histonet] GI Biopsies References: Message-ID: <5D13F5CAE20243D09F6D9F70F73CA9AA@pentium4> I think you're spot on with the slower cutting speed. Too fast and they "chatter" like crazy. Piero Nelva ----- Original Message ----- From: "Mickie Johnson" To: "'Evans, Andria B.'" ; Sent: Friday, November 14, 2008 8:50 AM Subject: RE: [Histonet] GI Biopsies Dear Andria, This sounds like over-processing. If you are using a regular processing schedule it is for sure. At Sacred Heart Medical Center in Spokane we used a 2 hour 45 minute program with 15 minutes in each station. We still soaked the blocks in water before icing and cutting. Sometimes we had to soak for the second or third level. We fixed in Bouin's. Also cutting with a slower speed as the knife passed through the tissue helped. Hope this helps Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing & Mohs Suite Mohs Reporting Software 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com & www.mohssuite.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. Thank You. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B. Sent: Thursday, November 13, 2008 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI Biopsies We are having difficulty with our GI biopsies having a lot of chatter on the H & E. I think somehow they are getting over processed, but not sure.... has anyone had this problem or could suggest what might be causing this and/or solutions. Thank you! Andria B Evans, HTL(ASCP)CM Anatomic Pathology York Hospital 1001 S. George Street York, PA 17405 717-851-5006 "You can learn a lot more from listening than you can from talking. Find someone with whom you don't agree in the slightest and ask them to explain themselves at length. Then take a seat, shut your mouth, and don't argue back. It's physically impossible to listen with your mouth open." -John Moe "Maturity is accepting imperfections." CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. ______________________________________________________________________ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.9.2/1783 - Release Date: 11/12/2008 10:01 AM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.9.3/1786 - Release Date: 11/13/2008 6:01 PM From gagnone <@t> KGH.KARI.NET Fri Nov 14 07:58:41 2008 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Fri Nov 14 07:58:53 2008 Subject: [Histonet] GI biopsies Message-ID: Andria, another factor may be trimming the GI biopsy blocks. If trimming increments are too large, it may rip some of the deeper tissue out of the block, causing holes or possibly the chatter you are seeing. Check the block after trimming to see if there is any unevenness to the surface. Also, is it limited to one person? If not, it may not be microtomy, look at processing as others have already mentioned. Hope this helps, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From CHRISH <@t> HEALTHCARESCOUTS.COM Fri Nov 14 08:17:08 2008 From: CHRISH <@t> HEALTHCARESCOUTS.COM (Chris Handrahan) Date: Fri Nov 14 08:25:15 2008 Subject: [Histonet] Current Histotech Job Opportunities Message-ID: The #1 Recruiter for Laboratory/Biotech Specialists Healthcare Scouts is a medical laboratory/biotech recruitment firm that places professionals in full-time, permanent positions throughout the United States. As the leading nationally recognized permanent placement solution for medical laboratory/biotech professionals, Healthcare Scouts has histotech openings in the following locations * Los Angeles, CA * Fort Wayne, IN * Charlotte, NC * Myrtle Beach, SC * Dallas, TX * San Antonio, TX * Seattle, WA * St Petersburg, FL * Baltimore, MD * Las Vegas, NV * Plattsburgh, NY * Charlottesville, VA * Roanoke, VA Let us Work on Your Career! Chris Handrahan Managing Director of Allied Health Healthcare Scouts 800-708-0605 office 321-231-5427 cell chrish@healthcarescouts.com www.healthcarescouts.com From cindipqr <@t> wowway.com Fri Nov 14 08:43:52 2008 From: cindipqr <@t> wowway.com (cindipqr@wowway.com) Date: Fri Nov 14 08:43:58 2008 Subject: [Histonet] Cryostat- information for new please Message-ID: <20081114143323.M81961@wowway.com> Hi All, including Vendors We're in the market for a new cryostat. Here are our needs: 1. Cutting rat and mouse brains. 2. Ability to keep temperature while cutting for a couple hours at a time. 3. Able to cut 8 to 20 microns. 4. Room in the cryostat for a 100 slide holder (to keep sections frozen after cutting. 5. Good price, as this is research. We don't need anything fancy. Thanks for any information you can give, Cindi Roberts HFH Neurology Research From michelle.steinkrauss <@t> novartis.com Fri Nov 14 08:48:35 2008 From: michelle.steinkrauss <@t> novartis.com (michelle.steinkrauss@novartis.com) Date: Fri Nov 14 08:48:49 2008 Subject: [Histonet] Michelle Steinkrauss is out of the office. Message-ID: I will be out of the office starting 11/14/2008 and will not return until 11/18/2008. If you require immediate assistance, please contact Michelle Broome at x 47477. From whitmang <@t> trinity-health.org Fri Nov 14 08:50:59 2008 From: whitmang <@t> trinity-health.org (Georgine Whitman) Date: Fri Nov 14 08:51:13 2008 Subject: [Histonet] Bone Marrow smears fixation Message-ID: <491D4A02.9B4B.00A7.0@trinity-health.org> we are trying to do an iron stain on our bone marrow smears on the Ventana Nexus. We are having trouble with the specimen staying on the slide. I personally have not done iron on smears in years. Our hematology department has been doing them by hand and they are fixed in methanol alcohol. Please Help Thank You Georgine From will <@t> histologytechservices.com Fri Nov 14 10:19:53 2008 From: will <@t> histologytechservices.com (William Connor) Date: Fri Nov 14 10:20:25 2008 Subject: [Histonet] Cryostat- information for new please In-Reply-To: <20081114143323.M81961@wowway.com> Message-ID: <0MKpCa-1L11P81zVB-0007wD@mrelay.perfora.net> The Microm HM 550 is an awesome cryostat, and meets requirements 1-3 for sure. Not sure about the price. I think we got ours used... There is room for a 100 count slide box, but you'd have to maneuver it a bit in order to get the lid open and a slide in there. William Connor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cindipqr@wowway.com Sent: Friday, November 14, 2008 9:44 AM To: Histonet Subject: [Histonet] Cryostat- information for new please Hi All, including Vendors We're in the market for a new cryostat. Here are our needs: 1. Cutting rat and mouse brains. 2. Ability to keep temperature while cutting for a couple hours at a time. 3. Able to cut 8 to 20 microns. 4. Room in the cryostat for a 100 slide holder (to keep sections frozen after cutting. 5. Good price, as this is research. We don't need anything fancy. Thanks for any information you can give, Cindi Roberts HFH Neurology Research _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From epoc101 <@t> gmail.com Fri Nov 14 11:21:31 2008 From: epoc101 <@t> gmail.com (Joe) Date: Fri Nov 14 11:21:40 2008 Subject: [Histonet] old electron microscopes Message-ID: <231dd8040811140921m6b9f4c37hc58c185567694477@mail.gmail.com> A freind askded me to post this question to the list and see if he could get any guidance. He has two electron microscopes that appear to this untrained eye to be older than the hills, or quite possibly alien artifacts. One is a Siemens unit (power supply is bigger than a fridge). The other, I believe is also a siemens unit, but it appears newer (relatively), than the other. It's a bit smaller and has an orange body to the tube part. He wants to get rid of them and wants to know if they have any value on today's market? I tried the histoauction, but the page did not seem be working. Any help would be appreciated. From shive003 <@t> umn.edu Fri Nov 14 11:33:15 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Nov 14 11:33:22 2008 Subject: [Histonet] old electron microscopes References: <231dd8040811140921m6b9f4c37hc58c185567694477@mail.gmail.com> Message-ID: Joe, You might try going to a listserv for Electron Microscopy. You can find it at: http://www.microscopy.com Jan Shivers ----- Original Message ----- From: "Joe" To: Sent: Friday, November 14, 2008 11:21 AM Subject: [Histonet] old electron microscopes >A freind askded me to post this question to the list and see if he could >get > any guidance. He has two electron microscopes that appear to this > untrained > eye to be older than the hills, or quite possibly alien artifacts. One is > a > Siemens unit (power supply is bigger than a fridge). The other, I believe > is also a siemens unit, but it appears newer (relatively), than the other. > It's a bit smaller and has an orange body to the tube part. He wants to > get > rid of them and wants to know if they have any value on today's market? I > tried the histoauction, but the page did not seem be working. Any help > would be appreciated. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jcline <@t> wchsys.org Fri Nov 14 11:39:40 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Nov 14 11:39:47 2008 Subject: [Histonet] Bone Marrow smears fixation In-Reply-To: <491D4A02.9B4B.00A7.0@trinity-health.org> Message-ID: We give our Hematology department Superfrost+ slides to use for smears, because we had the same problem. We also went back to the old fashioned way of fixing the smears. We use a coplin jar with a small piece of papertowel in the bottom and place a few drops of formalin in the coplin jar. The smears are fixed in formalin fumes. We had a lot of smears that were not staining for iron before using the methanol method. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Georgine Whitman Sent: Friday, November 14, 2008 9:51 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Bone Marrow smears fixation we are trying to do an iron stain on our bone marrow smears on the Ventana Nexus. We are having trouble with the specimen staying on the slide. I personally have not done iron on smears in years. Our hematology department has been doing them by hand and they are fixed in methanol alcohol. Please Help Thank You Georgine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Jackie.O'Connor <@t> abbott.com Fri Nov 14 11:52:09 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Nov 14 11:52:31 2008 Subject: [Histonet] old electron microscopes In-Reply-To: <231dd8040811140921m6b9f4c37hc58c185567694477@mail.gmail.com> Message-ID: Could you imagine these things on Ebay? Joe Sent by: histonet-bounces@lists.utsouthwestern.edu 11/14/2008 11:21 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] old electron microscopes A freind askded me to post this question to the list and see if he could get any guidance. He has two electron microscopes that appear to this untrained eye to be older than the hills, or quite possibly alien artifacts. One is a Siemens unit (power supply is bigger than a fridge). The other, I believe is also a siemens unit, but it appears newer (relatively), than the other. It's a bit smaller and has an orange body to the tube part. He wants to get rid of them and wants to know if they have any value on today's market? I tried the histoauction, but the page did not seem be working. Any help would be appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Fri Nov 14 12:05:16 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Nov 14 12:05:19 2008 Subject: [Histonet] old electron microscopes Message-ID: <793890.76565.qm@web1116.biz.mail.sk1.yahoo.com> I have seen EM scopes on Ebay, but the microscopy list would probably be a?better place to start. ________________________________ From: Jackie M O'Connor To: Joe Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Friday, November 14, 2008 11:52:09 AM Subject: Re: [Histonet] old electron microscopes Could you imagine these things on Ebay? Joe Sent by: histonet-bounces@lists.utsouthwestern.edu 11/14/2008 11:21 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] old electron microscopes A freind askded me to post this question to the list and see if he could get any guidance.? He has two electron microscopes that appear to this untrained eye to be older than the hills, or quite possibly alien artifacts.? One is a Siemens unit (power supply is bigger than a fridge).? The other, I believe is also a siemens unit, but it appears newer (relatively), than the other. It's a bit smaller and has an orange body to the tube part.? He wants to get rid of them and wants to know if they have any value on today's market?? I tried the histoauction, but the page did not seem be working.? Any help would be appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lloyd.3 <@t> osu.edu Fri Nov 14 12:06:22 2008 From: lloyd.3 <@t> osu.edu (Mary Lloyd) Date: Fri Nov 14 12:06:26 2008 Subject: [Histonet] Millon's/millions reagent Message-ID: <2096B6FC591D034FA50AFFD7A42EE96506775097@dental.dentnet.dent.ohio-state.edu> Does anyone in histoland know anything about Millons/millions reagent for Tyrosine granules. My pathologist is asking for this technique. Thanks Mary Lloyd From kbowden <@t> ucsd.edu Fri Nov 14 12:12:50 2008 From: kbowden <@t> ucsd.edu (kbowden) Date: Fri Nov 14 12:12:56 2008 Subject: [Histonet] Opinions on motorized microtomes wanted In-Reply-To: <231dd8040811140921m6b9f4c37hc58c185567694477@mail.gmail.com> References: <231dd8040811140921m6b9f4c37hc58c185567694477@mail.gmail.com> Message-ID: <491DBFA2.4000100@ucsd.edu> I'm looking to buy a motorized paraffin microtome. I have been looking to see what is out there, and there seems to be quit a few. I would like to get feedback from people who have used them. I have a problem with my shoulder and the continuous motion of using a rotory microtome is a issue for me. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. From jrobertson <@t> pathologysciences.com Fri Nov 14 12:15:17 2008 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Fri Nov 14 12:15:22 2008 Subject: [Histonet] Opinions on motorized microtomes wanted In-Reply-To: <491DBFA2.4000100@ucsd.edu> Message-ID: <518CD6920AA7154193CBE5977CD88073177D60@psmgsrv2.PSMG.local> The one from Microm is very good and easy to use. All the control buttons are on the microtome. There is also one from Leica that we have that is good, but it's a bit more cumbersome with the control panel attached to a keyboard and a cord to the left of the microtome. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Chico, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kbowden Sent: Friday, November 14, 2008 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinions on motorized microtomes wanted I'm looking to buy a motorized paraffin microtome. I have been looking to see what is out there, and there seems to be quit a few. I would like to get feedback from people who have used them. I have a problem with my shoulder and the continuous motion of using a rotory microtome is a issue for me. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andrea.ferullo <@t> spcorp.com Fri Nov 14 12:19:22 2008 From: andrea.ferullo <@t> spcorp.com (Ferullo, Andrea) Date: Fri Nov 14 12:19:29 2008 Subject: [Histonet] Opinions on motorized microtomes wanted In-Reply-To: <518CD6920AA7154193CBE5977CD88073177D60@psmgsrv2.PSMG.local> Message-ID: <737ABA3B15ADB74B81C8DB616201BAFF0415A05D@KENMSG20.us.schp.com> I've also used both of the automated ones just mentioned (Microm and Leica). Both are very easy to use. I would go with either of these. Andrea Ferullo Assistant Scientist Necropsy/Histology, Anatomic Pathology T: +1 973 940 4282 F: +1 973 940 4120 andrea.ferullo@spcorp.com Schering-Plough Research Institute 144 Route 94 Lafayette, NJ 07848 www.schering-plough.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jodie Robertson Sent: Friday, November 14, 2008 1:15 PM To: kbowden; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Opinions on motorized microtomes wanted The one from Microm is very good and easy to use. All the control buttons are on the microtome. There is also one from Leica that we have that is good, but it's a bit more cumbersome with the control panel attached to a keyboard and a cord to the left of the microtome. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Chico, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kbowden Sent: Friday, November 14, 2008 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinions on motorized microtomes wanted I'm looking to buy a motorized paraffin microtome. I have been looking to see what is out there, and there seems to be quit a few. I would like to get feedback from people who have used them. I have a problem with my shoulder and the continuous motion of using a rotory microtome is a issue for me. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From mbisher <@t> Princeton.EDU Fri Nov 14 12:22:32 2008 From: mbisher <@t> Princeton.EDU (Peggy Bisher) Date: Fri Nov 14 12:22:42 2008 Subject: [Histonet] old electron microscopes In-Reply-To: Message-ID: I have seen them there! Mostly SEM's. On 11/14/08 12:52 PM, "Jackie M O'Connor" wrote: > Could you imagine these things on Ebay? > > > > > Joe > Sent by: histonet-bounces@lists.utsouthwestern.edu > 11/14/2008 11:21 AM > > To > histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] old electron microscopes > > > > > > > A freind askded me to post this question to the list and see if he could > get > any guidance. He has two electron microscopes that appear to this > untrained > eye to be older than the hills, or quite possibly alien artifacts. One is > a > Siemens unit (power supply is bigger than a fridge). The other, I believe > is also a siemens unit, but it appears newer (relatively), than the other. > It's a bit smaller and has an orange body to the tube part. He wants to > get > rid of them and wants to know if they have any value on today's market? I > tried the histoauction, but the page did not seem be working. Any help > would be appreciated. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbisher <@t> Princeton.EDU Fri Nov 14 12:24:25 2008 From: mbisher <@t> Princeton.EDU (Peggy Bisher) Date: Fri Nov 14 12:24:35 2008 Subject: [Histonet] old electron microscopes In-Reply-To: <793890.76565.qm@web1116.biz.mail.sk1.yahoo.com> Message-ID: Actually I belong to the MSA and they have a place on their web page to list equipment for sale. If you know exactly what you have, then that would be the way to go. You will have to join though, to post it. It doesn't cost anything to join, just some information. Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher@princeton.edu On 11/14/08 1:05 PM, "Paula Pierce" wrote: > I have seen EM scopes on Ebay, but the microscopy list would probably be > a?better place to start. > > > > > ________________________________ > From: Jackie M O'Connor > To: Joe > Cc: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Sent: Friday, November 14, 2008 11:52:09 AM > Subject: Re: [Histonet] old electron microscopes > > Could you imagine these things on Ebay? > > > > > Joe > Sent by: histonet-bounces@lists.utsouthwestern.edu > 11/14/2008 11:21 AM > > To > histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] old electron microscopes > > > > > > > A freind askded me to post this question to the list and see if he could > get > any guidance.? He has two electron microscopes that appear to this > untrained > eye to be older than the hills, or quite possibly alien artifacts.? One is > a > Siemens unit (power supply is bigger than a fridge).? The other, I believe > is also a siemens unit, but it appears newer (relatively), than the other. > It's a bit smaller and has an orange body to the tube part.? He wants to > get > rid of them and wants to know if they have any value on today's market?? I > tried the histoauction, but the page did not seem be working.? Any help > would be appreciated. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lloyd.3 <@t> osu.edu Fri Nov 14 12:49:09 2008 From: lloyd.3 <@t> osu.edu (Mary Lloyd) Date: Fri Nov 14 12:49:14 2008 Subject: [Histonet] Millons Message-ID: <2096B6FC591D034FA50AFFD7A42EE96506775098@dental.dentnet.dent.ohio-state.edu> The request for Millons reagent is to stain Tyrosine crystals in formalin fixed paraffin embedded tissue. Does anyone have an alternative staining method for Tyrosine crystals Thank you. Mary Lloyd From jkiernan <@t> uwo.ca Fri Nov 14 13:07:40 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Nov 14 13:07:47 2008 Subject: [Histonet] Millon's/millions reagent Message-ID: Millon's reagent is used in a traditional test for proteins. It reacts with the side chain of tyrosine to form a coloured compound (red, pink, orange). All proteins contain tyrosine, so this is a stain for proteins in general. For histochemical use, JR Baker's modification is recommended: "The histochemical recognition of phenols, especially tyrosine" in Quart. J. Microsc. Sci. 97: 161-164 (1956). This paper can be freely downloaded from the internet: http://jcs.biologists.org/cgi/reprint/s3-97/38/161 The instructions are more detailed than those given for Millon's method in most techniques books. Be careful! Millon's reagent contains 10% mercuric sulphate. concentrated sulphuric acid is also needed, and the solution has to be boiled. Those of us who were schoolboys in an earlier age will remember putting copper coins into Millon's reagent. They quickly became plated with mercury, and resembled silver coins. John Kiernan Anatomy, UWO London, Canada. = = = ----- Original Message ----- From: Mary Lloyd Date: Friday, November 14, 2008 13:06 Subject: [Histonet] Millon's/millions reagent To: histonet@lists.utsouthwestern.edu > Does anyone in histoland know anything about Millons/millions reagent > for Tyrosine granules. My pathologist is asking for this > technique.Thanks Mary Lloyd > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nyilmaz <@t> mersin.edu.tr Fri Nov 14 14:22:01 2008 From: nyilmaz <@t> mersin.edu.tr (Nejat Yilmaz) Date: Fri Nov 14 14:22:19 2008 Subject: [Histonet] Nikon Diaphot 300 Message-ID: <001101c94696$a726daf0$2101a8c0@nejat1> Hello All, Could any of you provide an user or an instruction manual of a Nikon Diaphot 300 inverted fluorescence microscope preferably as a pdf file? Thanks in advance. Necat Yilmaz From SSCALISE <@t> beaumonthospitals.com Fri Nov 14 14:56:23 2008 From: SSCALISE <@t> beaumonthospitals.com (Sharon Scalise) Date: Fri Nov 14 14:57:55 2008 Subject: [Histonet] Dako Link True Positive ID Message-ID: <491D9FA6.1CA9.00C8.0@beaumonthospitals.com> We are investigating systems for specimen tracking using bar-code technology (including cassette and slide printers). Does anyone have any experience with the Dako Link True Positive ID system? Apparently it is not out in the U.S. as much as in Europe (according to Dako). Any information would be great (on the Dako system or any others out there). Have a great Week-End! Sharon E. Scalise, HTL (ASCP) Histology Supervisor William Beaumont Hospital Royal Oak, MI 48073 248 898-5981 From barrickstacey <@t> yahoo.com Fri Nov 14 15:12:44 2008 From: barrickstacey <@t> yahoo.com (Stacey Barrick) Date: Fri Nov 14 15:12:49 2008 Subject: [Histonet] Nikon Diaphot 300 In-Reply-To: <001101c94696$a726daf0$2101a8c0@nejat1> Message-ID: <178988.72275.qm@web54303.mail.re2.yahoo.com> Necat, I have just sent you the pdf?from my other email address. Let me know if?it doesnt go through. ? Have a great weekend! --- On Fri, 11/14/08, Nejat Yilmaz wrote: From: Nejat Yilmaz Subject: [Histonet] Nikon Diaphot 300 To: histonet@lists.utsouthwestern.edu Date: Friday, November 14, 2008, 3:22 PM Hello All, Could any of you provide an user or an instruction manual of a Nikon Diaphot 300 inverted fluorescence microscope preferably as a pdf file? Thanks in advance. Necat Yilmaz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmweber113 <@t> comcast.net Fri Nov 14 17:53:12 2008 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Fri Nov 14 17:53:20 2008 Subject: [Histonet] Thermo Shandon Microwave Message-ID: <111420082353.15828.491E0F68000A37D700003DD42216566276CCCECE9D0A0D0A99039D@comcast.net> Has anyone had consistant trouble processing GI Bx with the Thermo Shandon Tissue Wave 2 Microwave. I have a client who is getting over and under processed tissue in the same run. Their tech support can not seem to help. Thanks From kbowden <@t> ucsd.edu Fri Nov 14 18:02:51 2008 From: kbowden <@t> ucsd.edu (kbowden) Date: Fri Nov 14 18:02:56 2008 Subject: [Histonet] Opinions: motorized microtomes Thank you!! In-Reply-To: <491DBFA2.4000100@ucsd.edu> References: <231dd8040811140921m6b9f4c37hc58c185567694477@mail.gmail.com> <491DBFA2.4000100@ucsd.edu> Message-ID: <491E11AB.8040105@ucsd.edu> Thank you for your responces. They have been very helpful. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. kbowden wrote: > I'm looking to buy a motorized paraffin microtome. I have been > looking to see what is out there, and there seems to be quit a few. I > would like to get feedback from people who have used them. I have a > problem with my shoulder and the continuous motion of using a rotory > microtome is a issue for me. > -- > Karen Bowden > Staff Research Associate II > University of CA, San Diego > Department of Orthopedics > 9500 Gilman Dr. 0630 > La Jolla, CA 92093-0630 > 858-534-4655 voice > 858-534-5304 fax > > > CONFIDENTIALITY NOTICE: > THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE > PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL > AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR > OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION > BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. > IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND > DELETE THE MATERIAL FROM ANY COMPUTER. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From RSRICHMOND <@t> aol.com Fri Nov 14 21:44:01 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Nov 14 21:44:05 2008 Subject: [Histonet] Re: Buffer. Message-ID: Re this thread on buffering: cacodylate buffering is frequently used with glutaraldehyde for electron microscopy. Nobody ever seems to mention it, but cacodylate (cacodylic acid) contains organic arsenic, and is extremely toxic. See http://en.wikipedia.org/wiki/Cacodylate What precautions are advisable in handling it? Is it permitted in a hospital laboratory? I've been reading about a procedure that may require it - will post more information if I receive it from the author. Bob Richmond Samurai Pathologist Knoxville TN From cindipqr <@t> wowway.com Sat Nov 15 11:13:54 2008 From: cindipqr <@t> wowway.com (cindipqr@wowway.com) Date: Sat Nov 15 11:13:59 2008 Subject: [Histonet] Thanks for Cryostat information Message-ID: <20081115171227.M36452@wowway.com> Thank you to all for the cryostat information! Cindi From leahcox27 <@t> yahoo.com Sat Nov 15 12:27:10 2008 From: leahcox27 <@t> yahoo.com (Leah Cox) Date: Sat Nov 15 12:27:16 2008 Subject: [Histonet] ASCP certification question Message-ID: <799783.15509.qm@web54603.mail.re2.yahoo.com> How do we know what histology programs or schools can get us the ASCP? Phoenix College has a program but it's not on the NSH list of schools. Is there a reason for that? From rjbuesa <@t> yahoo.com Sat Nov 15 12:38:45 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Nov 15 12:38:49 2008 Subject: [Histonet] ASCP certification question In-Reply-To: <799783.15509.qm@web54603.mail.re2.yahoo.com> Message-ID: <980211.25632.qm@web65713.mail.ac4.yahoo.com> Go to the NACCLS web site and there you will find all the certified programs. Sometimes a program exists but has not the certification for internal reasons. Ren? J. --- On Sat, 11/15/08, Leah Cox wrote: From: Leah Cox Subject: [Histonet] ASCP certification question To: "histonet@lists.utsouthwestern.edu" Date: Saturday, November 15, 2008, 1:27 PM How do we know what histology programs or schools can get us the ASCP? Phoenix College has a program but it's not on the NSH list of schools. Is there a reason for that? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Sat Nov 15 15:47:10 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sat Nov 15 15:47:15 2008 Subject: [Histonet] ASCP certification question In-Reply-To: <980211.25632.qm@web65713.mail.ac4.yahoo.com> Message-ID: <714267.15562.qm@web50903.mail.re2.yahoo.com> http://www.naacls.org/ ? http://www.naacls.org/accreditation/ht/links.asp ? Hey Leah-- ? Programs are certified and certification is maintained by NAACLS.? If a school doesn't apply and go through the process or is not recertified when their renewal is due, they aren't accredited.? ASCP requires accredited?education. ?It's like a lab and the CAP or JCAHO....it takes time and a lot of effort and additional costs?to be certified. ? You can get the education and be well-qualified to do the work through other programs, but why would you go through all that effort and expense if you can't sit for the 'gold standard' of the ASCP exam?? ? The links above will help--give me a call if you need more! ? Cheryl ? Full Staff Inc. Staffing Healthcare Professionals - one GREAT fit at a time. 800.756.3309 ? You do really want to work through an accredited program.? Several are internet-based including the one out of Hartford CT and another out of Indiana University.? --- On Sat, 11/15/08, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] ASCP certification question To: "histonet@lists.utsouthwestern.edu" , leahcox27@yahoo.com Date: Saturday, November 15, 2008, 10:38 AM Go to the NACCLS web site and there you will find all the certified programs. Sometimes a program exists but has not the certification for internal reasons. Ren? J. --- On Sat, 11/15/08, Leah Cox wrote: From: Leah Cox Subject: [Histonet] ASCP certification question To: "histonet@lists.utsouthwestern.edu" Date: Saturday, November 15, 2008, 1:27 PM How do we know what histology programs or schools can get us the ASCP? Phoenix College has a program but it's not on the NSH list of schools. Is there a reason for that? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Sat Nov 15 22:23:38 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sat Nov 15 22:23:43 2008 Subject: [Histonet] ASCP certification question In-Reply-To: <714267.15562.qm@web50903.mail.re2.yahoo.com> Message-ID: Programs are accredited, not certified. Cheryl Sent by: histonet-bounces@lists.utsouthwestern.edu 11/15/2008 01:48 PM To "histonet@lists.utsouthwestern.edu" , leahcox27@yahoo.com, rjbuesa@yahoo.com cc Subject Re: [Histonet] ASCP certification question http://www.naacls.org/ http://www.naacls.org/accreditation/ht/links.asp Hey Leah-- Programs are certified and certification is maintained by NAACLS. If a school doesn't apply and go through the process or is not recertified when their renewal is due, they aren't accredited. ASCP requires accredited education. It's like a lab and the CAP or JCAHO....it takes time and a lot of effort and additional costs to be certified. You can get the education and be well-qualified to do the work through other programs, but why would you go through all that effort and expense if you can't sit for the 'gold standard' of the ASCP exam?? The links above will help--give me a call if you need more! Cheryl Full Staff Inc. Staffing Healthcare Professionals - one GREAT fit at a time. 800.756.3309 You do really want to work through an accredited program. Several are internet-based including the one out of Hartford CT and another out of Indiana University. --- On Sat, 11/15/08, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] ASCP certification question To: "histonet@lists.utsouthwestern.edu" , leahcox27@yahoo.com Date: Saturday, November 15, 2008, 10:38 AM Go to the NACCLS web site and there you will find all the certified programs. Sometimes a program exists but has not the certification for internal reasons. Ren? J. --- On Sat, 11/15/08, Leah Cox wrote: From: Leah Cox Subject: [Histonet] ASCP certification question To: "histonet@lists.utsouthwestern.edu" Date: Saturday, November 15, 2008, 1:27 PM How do we know what histology programs or schools can get us the ASCP? Phoenix College has a program but it's not on the NSH list of schools. Is there a reason for that? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jtrynak <@t> hotmail.com Sun Nov 16 12:00:29 2008 From: jtrynak <@t> hotmail.com (jtrynak@hotmail.com) Date: Sun Nov 16 12:00:34 2008 Subject: [Histonet] Vacation reply In-Reply-To: Message-ID: Hey?
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Regards
?
From histonetalias <@t> gmail.com Sun Nov 16 12:26:55 2008 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Sun Nov 16 12:26:59 2008 Subject: [Histonet] Dako Link True Positive ID In-Reply-To: <491D9FA6.1CA9.00C8.0@beaumonthospitals.com> References: <491D9FA6.1CA9.00C8.0@beaumonthospitals.com> Message-ID: <4b6c85510811161026y381a1928v332c0c6afd4ddcb@mail.gmail.com> Have you looked into the Ventana "Vantage" system yet? I saw a demo of this and it looks pretty impressive. On Fri, Nov 14, 2008 at 3:56 PM, Sharon Scalise < SSCALISE@beaumonthospitals.com> wrote: > We are investigating systems for specimen tracking using bar-code > technology (including cassette and slide printers). Does anyone have any > experience with the Dako Link True Positive ID system? Apparently it is not > out in the U.S. as much as in Europe (according to Dako). Any information > would be great (on the Dako system or any others out there). > > Have a great Week-End! > > > Sharon E. Scalise, HTL (ASCP) > Histology Supervisor > William Beaumont Hospital > Royal Oak, MI 48073 > 248 898-5981 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- The Unknown HT(ASCP) From RSRICHMOND <@t> aol.com Sun Nov 16 13:12:09 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Sun Nov 16 13:12:15 2008 Subject: [Histonet] Re: Buffer. Message-ID: About the potential toxicity of arsenic-containing cacodylate buffers: A Histonetter who prefers to remain anonymous notes >>The arsenic discussion comes up on the Microscopy Listserver every once in while, usually because someone is either pregnant & concerned about exposure or has safety officers who freak out over the stuff. It was used in the early days because phosphate buffers can interact with some of the fixative combinations and leave precipitates. Also, I'd suspect, cacodylate is less prone to beasties growing in stock solutions over time. The "modern" zwitterionic buffers are used a lot these days - PIPES & that crowd - if you want to avoid cacodylate & phosphate.<< Those zwitterionic buffers are often referred to as Good's buffers or Good buffers, since they were introduced by Norman Good in the 1960's. See http://en.wikipedia.org/wiki/Good's_buffers I don't know why they aren't commonly used as buffers for histologic fixatives. Bob Richmond Samurai Pathologist Knoxville TN From lpwenk <@t> sbcglobal.net Sun Nov 16 20:25:02 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Nov 16 20:25:31 2008 Subject: [Histonet] ASCP certification question In-Reply-To: <714267.15562.qm@web50903.mail.re2.yahoo.com> Message-ID: <001801c9485b$b31e21d0$0202a8c0@HPPav2> Just a FYI - There are two routes to be eligible for sit for the HT exam through ASCP: Route 1 which is completing a NAACLS accredited HT program, and Route 2 which is the on-the-job-training (OJT) route, which requires an associate degree, 12 hours of chemistry and biology, and 1 full time experience. (See link below) If you go through a NAACLS accredited HT program, upon completion, you are instantly eligible to sit for the HT certification exam through ASCP. On the other hand, if you earn an associate degree with the correct bio/chem classes, get hired by a histology lab where you work full time for 1 year doing fixation, processing, microtome and staining, then you can take the ASCP HT exam via route 2 OJT. I don't know about the Phoenix program, but possibly they are just trying to give people the associate degree with bio and chem courses related to laboratories and histology, so that a histology lab might look more favorably about hiring that person, so that they can gain experience for the OJT route. On the third hand, the Phoenix College website does say that Phoenix College is applying for accreditation through NAACLS. I would suggest you check with NAACLS, to see if the college has indeed applied. While clicking around, I found an April 2005 website on Phoenix College that said the same thing. So that indicates to me that the college has been "planning" on applying for several years, so I wonder if and when they really are going to do it. So call NAACLS at 773-714-8880 to find out where they are in the process. The following is the link to the ASCP HT certification exam requirement: http://www.ascp.org/FunctionalNavigation/certification/GetCertified/Technici anCertification.aspx To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Successful completion of a NAACLS accredited Histotechnician program within the last 5 years prior to the date of application for examination; or Route 2: Associate degree or at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last ten years under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology), or an appropriately board certified medical scientist. Laboratory Experience To fulfill the experience requirement for the Histotechnician examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining Lee & Peggy Wenk -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Saturday, November 15, 2008 4:47 PM To: histonet@lists.utsouthwestern.edu; leahcox27@yahoo.com; rjbuesa@yahoo.com Subject: Re: [Histonet] ASCP certification question http://www.naacls.org/ ? http://www.naacls.org/accreditation/ht/links.asp ? Hey Leah-- ? Programs are certified and certification is maintained by NAACLS.? If a school doesn't apply and go through the process or is not recertified when their renewal is due, they aren't accredited.? ASCP requires accredited?education. ?It's like a lab and the CAP or JCAHO....it takes time and a lot of effort and additional costs?to be certified. ? You can get the education and be well-qualified to do the work through other programs, but why would you go through all that effort and expense if you can't sit for the 'gold standard' of the ASCP exam?? ? The links above will help--give me a call if you need more! ? Cheryl ? Full Staff Inc. Staffing Healthcare Professionals - one GREAT fit at a time. 800.756.3309 ? You do really want to work through an accredited program.? Several are internet-based including the one out of Hartford CT and another out of Indiana University.? --- On Sat, 11/15/08, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] ASCP certification question To: "histonet@lists.utsouthwestern.edu" , leahcox27@yahoo.com Date: Saturday, November 15, 2008, 10:38 AM Go to the NACCLS web site and there you will find all the certified programs. Sometimes a program exists but has not the certification for internal reasons. Ren? J. --- On Sat, 11/15/08, Leah Cox wrote: From: Leah Cox Subject: [Histonet] ASCP certification question To: "histonet@lists.utsouthwestern.edu" Date: Saturday, November 15, 2008, 1:27 PM How do we know what histology programs or schools can get us the ASCP? Phoenix College has a program but it's not on the NSH list of schools. Is there a reason for that? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Mon Nov 17 06:34:36 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Nov 17 06:34:43 2008 Subject: [Histonet] dakowoes Message-ID: <7722595275A4DD4FA225B92CDBF174A1744E1B45EF@EXC-MBX3.cfs.le.ac.uk> We need to replace the soon tobe nomore DAKO Duet Kit, and the DAKO StreptABComplex/HRP(K 0377), any suggestions gratefully received. Richard Edwards University of Leicester U.K..... From SwainFrancesL <@t> uams.edu Mon Nov 17 06:48:55 2008 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Mon Nov 17 06:51:18 2008 Subject: [Histonet] Opinions on motorized microtomes wanted In-Reply-To: <491DBFA2.4000100@ucsd.edu> References: <231dd8040811140921m6b9f4c37hc58c185567694477@mail.gmail.com> <491DBFA2.4000100@ucsd.edu> Message-ID: Hi Karen: I have both microtomes. I use them to cut undecalcified MMA sections. I like the Leica 2255 the best. I have a friend in our business that uses to cut paraffin sections and thinks it is the best she has ever used. I am almost in agreement with her although the Microm is also a wonderful microtome. My suggestions is to get in touch with the sales rep for both companies and see if they will let you test the microtomes you are looking at to use. That way you can get the one that is the best for you. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kbowden Sent: Friday, November 14, 2008 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinions on motorized microtomes wanted I'm looking to buy a motorized paraffin microtome. I have been looking to see what is out there, and there seems to be quit a few. I would like to get feedback from people who have used them. I have a problem with my shoulder and the continuous motion of using a rotory microtome is a issue for me. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Bonnie.Whitaker <@t> osumc.edu Mon Nov 17 08:25:40 2008 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Mon Nov 17 08:25:53 2008 Subject: [Histonet] Meeting in Columbus, OH Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F60801CBD@msxc06.OSUMC.EDU> Hi Everyone! I wanted to let folks know that Dec. 6, we are having a CEU meeting at Ohio State University. The class is "Introduction to Immuno" and is geared to those with little to no experience in IHC. Please email me for details if you are interested in attending. Thanks! Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 Bonnie.Whitaker@osumc.edu phone 614.293.5048 fax 614.293.7273 pager 614.346.5013 From jcline <@t> wchsys.org Mon Nov 17 08:41:37 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Mon Nov 17 08:41:58 2008 Subject: [Histonet] Bone Marrow smears fixation In-Reply-To: Message-ID: <30CA7E0671234B0F8A46D6F5EA7DB35C@wchsys.org> Toni, They are fixed for 10 minutes in the fomalin fumes. We do not perform IHC on bone marrow smears, sorry Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Friday, November 14, 2008 1:30 PM To: Joyce Cline Subject: RE: [Histonet] Bone Marrow smears fixation Joyce, How long do you let the slides stay with the fumes? Have you run IHC on slides fixed this way? Thanks, Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joyce Cline Sent: Friday, November 14, 2008 12:40 PM To: Histonet Subject: RE: [Histonet] Bone Marrow smears fixation We give our Hematology department Superfrost+ slides to use for smears, because we had the same problem. We also went back to the old fashioned way of fixing the smears. We use a coplin jar with a small piece of papertowel in the bottom and place a few drops of formalin in the coplin jar. The smears are fixed in formalin fumes. We had a lot of smears that were not staining for iron before using the methanol method. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Georgine Whitman Sent: Friday, November 14, 2008 9:51 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Bone Marrow smears fixation we are trying to do an iron stain on our bone marrow smears on the Ventana Nexus. We are having trouble with the specimen staying on the slide. I personally have not done iron on smears in years. Our hematology department has been doing them by hand and they are fixed in methanol alcohol. Please Help Thank You Georgine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From MMargiotta <@t> bmhmc.org Mon Nov 17 11:37:19 2008 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Mon Nov 17 11:37:23 2008 Subject: [Histonet] breast bx processing Message-ID: Hi All, We are trying to figure out how to adjust our processing schedule for breast core bxs on the weekends so that they are not sitting in formalin for too long. Just wondering what everyone else is doing. Your input would be very helpful. Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From mwich <@t> 7thwavelabs.com Mon Nov 17 11:56:45 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Mon Nov 17 11:56:49 2008 Subject: [Histonet] Oil Red O despair Message-ID: <62A8156F8071C8439080D626DF8C33A602E554@wave-mail.7thwave.local> I'm wondering what are the pros (or cons) of using the propylene glycol version of Oil Red O over the isopropanol method. Is one more suited to a specific application? I'm trying to stain a frozen section of liver, which one would suspect would be loaded with fat, and have thus far been unsuccessful using the propylene glycol Oil Red O. Is there something obvious that I'm missing here? I'm new to cryosectioning...perhaps I did something wrong in the cryostat. I fixed my sections in NBF before staining. Please help. I'm feeling like a pathetic excuse for a histotech. Any advice is greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From jhnspam <@t> aol.com Mon Nov 17 12:03:18 2008 From: jhnspam <@t> aol.com (pam johnson) Date: Mon Nov 17 12:03:47 2008 Subject: [Histonet] Oil Red O despair In-Reply-To: <62A8156F8071C8439080D626DF8C33A602E554@wave-mail.7thwave.local> References: <62A8156F8071C8439080D626DF8C33A602E554@wave-mail.7thwave.local> Message-ID: <8CB16F5E75964AC-B4C-EA@webmail-db21.sysops.aol.com> Michele, I am having the same issues. I cannot get a good stain. I am getting alot of non-specific staining. I am using the kit from PolyScientific. I am using mouse tissue. I have never had so many problems with an Oil Red O. It is such a simple stain. -----Original Message----- From: Michele Wich To: histonet@lists.utsouthwestern.edu Sent: Mon, 17 Nov 2008 11:56 am Subject: [Histonet] Oil Red O despair I'm wondering what are the pros (or cons) of using the propylene glycol version of Oil Red O over the isopropanol method. Is one more suited to a specific application? I'm trying to stain a frozen section of liver, which one would suspect would be loaded with fat, and have thus far been unsuccessful using the propylene glycol Oil Red O. Is there something obvious that I'm missing here? I'm new to cryosectioning...perhaps I did something wrong in the cryostat. I fixed my sections in NBF before staining. Please help. I'm feeling like a pathetic excuse for a histotech. Any advice is greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From derek.papalegis <@t> tufts.edu Mon Nov 17 12:10:21 2008 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Mon Nov 17 12:10:27 2008 Subject: [Histonet] Oil Red O despair In-Reply-To: <8CB16F5E75964AC-B4C-EA@webmail-db21.sysops.aol.com> References: <62A8156F8071C8439080D626DF8C33A602E554@wave-mail.7thwave.local> <8CB16F5E75964AC-B4C-EA@webmail-db21.sysops.aol.com> Message-ID: <4921B38D.1080006@tufts.edu> I have had much better luck staining liver samples with Sudan Black B rather than with Oil Red O. I suggest giving that a try. Derek Papalegis HT (ASCP) Senior Histology Technologist Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 pam johnson wrote: > Michele, > > I am having the same issues. I cannot get a good stain. I am getting alot of non-specific staining. I am using the kit from PolyScientific. I am using mouse tissue. I have never had so many problems with an Oil Red O. It is such a simple stain. > > > > > > > > -----Original Message----- > From: Michele Wich > To: histonet@lists.utsouthwestern.edu > Sent: Mon, 17 Nov 2008 11:56 am > Subject: [Histonet] Oil Red O despair > > > > I'm wondering what are the pros (or cons) of using the propylene glycol > version of Oil Red O over the isopropanol method. Is one more suited to > a specific application? > > I'm trying to stain a frozen section of liver, which one would suspect > would be loaded with fat, and have thus far been unsuccessful using the > propylene glycol Oil Red O. > > Is there something obvious that I'm missing here? I'm new to > cryosectioning...perhaps I did something wrong in the cryostat. I fixed > my sections in NBF before staining. > > Please help. I'm feeling like a pathetic excuse for a histotech. Any > advice is greatly appreciated. > > > This communication is intended solely for the use of the addressee and may > contain information that is legally privileged, confidential or exempt from > disclosure. If you are not the intended recipient, please note that any > dissemination, distribution, or copying of this communication is strictly > prohibited. Anyone who receives this message in error should notify the sender > immediately and delete it from his or her computer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kim <@t> medequipsource.com Mon Nov 17 12:49:15 2008 From: kim <@t> medequipsource.com (Kim) Date: Mon Nov 17 12:49:49 2008 Subject: [Histonet] (no subject) Message-ID: <71A36250328DA34495AA0F972AE170C811B2AF@sbs01.MedicalEquipment.local> Hi all, We are currently looking for a used Leica 2125 and a used Leica 2135 Microtome. Any help in this would be great. Kimberly Henneberg 364 Mars-Valencia Rd. Mars, PA 16046 724-369-1604 www.kim@medequipsource.com From ktuttle <@t> umm.edu Mon Nov 17 12:52:00 2008 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Mon Nov 17 12:52:34 2008 Subject: [Histonet] Who inspects research labs? In-Reply-To: <799783.15509.qm@web54603.mail.re2.yahoo.com> References: <799783.15509.qm@web54603.mail.re2.yahoo.com> Message-ID: <492176FF.90CE.001A.3@umm.edu> Does anyone know what agency inspects research labs? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From talulahgosh <@t> gmail.com Mon Nov 17 12:55:06 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Nov 17 12:55:11 2008 Subject: [Histonet] Who inspects research labs? In-Reply-To: <492176FF.90CE.001A.3@umm.edu> References: <799783.15509.qm@web54603.mail.re2.yahoo.com> <492176FF.90CE.001A.3@umm.edu> Message-ID: If you're at a university, both IACUC and Environmental Health and Safety will. Outside of academics, I have no idea. Emily -- Millions of creatures walk the earth Unseen, both when we wake and when we sleep. -John Milton From Jackie.O'Connor <@t> abbott.com Mon Nov 17 13:01:29 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Nov 17 13:01:51 2008 Subject: [Histonet] Who inspects research labs? In-Reply-To: Message-ID: We are a pharma company, so the FDA visits us. IACUC doesn't inspect my lab, tho. "Emily Sours" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/17/2008 12:55 PM To histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] Who inspects research labs? If you're at a university, both IACUC and Environmental Health and Safety will. Outside of academics, I have no idea. Emily -- Millions of creatures walk the earth Unseen, both when we wake and when we sleep. -John Milton _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Vacca <@t> HCAhealthcare.com Mon Nov 17 13:05:21 2008 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Mon Nov 17 13:05:28 2008 Subject: [Histonet] JCAHO question Message-ID: <938D716CD445614ABBB817517557B6F439DF4573@NADCWPMSGCMS09.hca.corpad.net> I was presented by my lab manager this question when it comes to F.S. and FNA's, - The key word here is THE ORDERING OF: Does anyone monitor the time in which the specimen is removed from the OR to the time it is called back. Our specimens are delivered to us and clocked in at the time of receipt by the lab to the time in which it is resulted back. In CAP it does not state the "start" in which the TAT is monitored. We are both CAP and JCAHO, Do you have 2 separate policies or do you just use your CAP policy? NPSG.02.03.01 The [organization] measures, assesses, and, if needed, takes action to improve the timeliness of reporting, and the timeliness of receipt of critical tests and critical results and values by the responsible licensed caregiver. Elements of Performance for NPSG.02.03.01 1 The laboratory defines critical tests and critical results and values. 2 The laboratory defines the acceptable length of time between the ordering of critical tests and reporting the results of these tests, whether normal or abnormal. 3 The laboratory defines the acceptable length of time for reporting the results of routine tests with critical abnormal values or findings. 4 The laboratory defines the acceptable length of time between the availability of critical tests and critical results and values and receipt by the responsible licensed caregiver. 5 The laboratory collects data on the timeliness of reporting critical test results and critical results and values from routine tests. 6 The laboratory assesses the data on the timeliness of reporting critical test results and critical results and values from routine tests and determines whether a need for improvement exists. 7 The laboratory takes appropriate action to improve the timeliness of reporting critical test results and critical results and values from routine tests and measures the effectiveness of those actions. 8 Critically abnormal test results are communicated quickly to a responsible licensed caregiver so that prompt action may be taken. 9 When the responsible licensed caregiver is not available, a back-up reporting system provides the information in a timely manner to another qualified responsible caregiver to prevent avoidable delays in treatment or response. Jessica Vacca Histology Supervisor 119 Oakfield Dr Brandon Fl 33511 (813) 571-5193 (813) 571-5169 FAX ? From bakevictoria <@t> gmail.com Mon Nov 17 13:06:15 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Mon Nov 17 13:06:19 2008 Subject: [Histonet] Who inspects research labs? In-Reply-To: References: <799783.15509.qm@web54603.mail.re2.yahoo.com> <492176FF.90CE.001A.3@umm.edu> Message-ID: <4f016b690811171106r9f10b2fs46a854c15c122c3c@mail.gmail.com> Hi Emily, so you are not inspected by CAP then? Vikki Baker On Mon, Nov 17, 2008 at 1:55 PM, Emily Sours wrote: > If you're at a university, both IACUC and Environmental Health and Safety > will. > Outside of academics, I have no idea. > > Emily > -- > Millions of creatures walk the earth > Unseen, both when we wake and when we sleep. > -John Milton > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gmartin <@t> marshallmedical.org Mon Nov 17 13:14:27 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Mon Nov 17 13:14:32 2008 Subject: [Histonet] breast bx processing In-Reply-To: References: Message-ID: <6ED9D4252F278841A0593D3D788AF24C03C74CA4@mailsvr.MARSHMED.local> We come in and process them ! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, Michele Sent: Monday, November 17, 2008 9:37 AM To: histonet@pathology.swmed.edu Subject: [Histonet] breast bx processing Hi All, We are trying to figure out how to adjust our processing schedule for breast core bxs on the weekends so that they are not sitting in formalin for too long. Just wondering what everyone else is doing. Your input would be very helpful. Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Mon Nov 17 13:19:09 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Nov 17 13:19:14 2008 Subject: [Histonet] Who inspects research labs? In-Reply-To: <4f016b690811171106r9f10b2fs46a854c15c122c3c@mail.gmail.com> References: <799783.15509.qm@web54603.mail.re2.yahoo.com> <492176FF.90CE.001A.3@umm.edu> <4f016b690811171106r9f10b2fs46a854c15c122c3c@mail.gmail.com> Message-ID: We aren't a pathology lab, so no. Unless CAP doesn't stand for College of American Pathologists. Emily On Mon, Nov 17, 2008 at 2:06 PM, Victoria Baker wrote: > Hi Emily, > > so you are not inspected by CAP then? > > Vikki Baker > > -- Millions of creatures walk the earth Unseen, both when we wake and when we sleep. -John Milton From plucas <@t> biopath.org Mon Nov 17 13:32:19 2008 From: plucas <@t> biopath.org (Paula Lucas) Date: Mon Nov 17 13:32:22 2008 Subject: [Histonet] Per Diem Orange County California Message-ID: <20081117193219.33F1417E8@victory.cnchost.com> Histology lab seeking a per diem histotech who can work a few hours in the morning during vacation coverage. If you work either the night shift or a mid shift and want extra cash, this job would work for you. We look for individuals who have experience and who work the night shift or mid day shift at their full time jobs. You can come either after your shift is over if you work the night shift or before starting your work day. All we need is a few hours to get through the day. Please let me know if you have any questions. Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA From mucram11 <@t> comcast.net Mon Nov 17 13:36:47 2008 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Mon Nov 17 13:36:52 2008 Subject: [Histonet] Who inspects research labs? In-Reply-To: <686118265.105971226950318611.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <1559050730.107071226950607962.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> It depends on the laboratory and what you are researching.? It could be IRB if you are in a hospital setting and if it is also the histology lab for the hospital you may get CAP too.? At the Vet School we don't get IACUC unless?the PI writes it in a way that we have to be inspected, which is rare to nonexistent .? However we get the University safety group and GLP inspections from sponsors.? Who inspects you is really a reflection of what you are doing and as Jackie said pharma gets FDA and as GLP we can get them to for implants or possible human use products.? We get inspections from study sponsors and academics we do work for so it can be quite a large group and they all want something different.? Pam Marcum ----- Original Message ----- From: "Jackie M O'Connor" To: "Emily Sours" < talulahgosh @ gmail .com> Cc: histonet @lists. utsouthwestern . edu , histonet -bounces@lists. utsouthwestern . edu Sent: Monday, November 17, 2008 2:01:29 PM GMT -05:00 US/Canada Eastern Subject: Re: [ Histonet ] Who inspects research labs? We are a pharma company, so the FDA visits us. ? IACUC doesn't inspect my lab, tho. "Emily Sours" < talulahgosh @ gmail .com> Sent by: histonet -bounces@lists. utsouthwestern . edu 11/17/2008 12:55 PM To histonet @lists. utsouthwestern . edu cc Subject Re: [ Histonet ] Who inspects research labs? If you're at a university, both IACUC and Environmental Health and Safety will. Outside of academics, I have no idea. Emily -- Millions of creatures walk the earth Unseen, both when we wake and when we sleep. -John Milton _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet _______________________________________________ Histonet mailing list Histonet @lists. utsouthwestern . edu http ://lists. utsouthwestern . edu /mailman/ listinfo / histonet From akbitting <@t> geisinger.edu Mon Nov 17 14:27:49 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Nov 17 14:28:03 2008 Subject: [Histonet] breast bx processing In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C03C74CA4@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C03C74CA4@mailsvr.MARSHMED.local> Message-ID: <49218D75.2B7F.00C9.0@geisinger.edu> We have staff in on Sunday afternoon, so we start them Friday night and let them sit in paraffin until the techs come in on Sunday. The extra paraffin has actually cut down the number of dry outs we were having. >>> "Martin, Gary" 11/17/2008 2:14 PM >>> We come in and process them ! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, Michele Sent: Monday, November 17, 2008 9:37 AM To: histonet@pathology.swmed.edu Subject: [Histonet] breast bx processing Hi All, We are trying to figure out how to adjust our processing schedule for breast core bxs on the weekends so that they are not sitting in formalin for too long. Just wondering what everyone else is doing. Your input would be very helpful. Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Jackie.O'Connor <@t> abbott.com Mon Nov 17 14:41:14 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Nov 17 14:41:39 2008 Subject: [Histonet] breast bx processing In-Reply-To: <49218D75.2B7F.00C9.0@geisinger.edu> Message-ID: How long are they in molten paraffin???? "Angela Bitting" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/17/2008 02:27 PM To "Michele Margiotta" , "Gary Martin" , cc Subject RE: [Histonet] breast bx processing We have staff in on Sunday afternoon, so we start them Friday night and let them sit in paraffin until the techs come in on Sunday. The extra paraffin has actually cut down the number of dry outs we were having. >>> "Martin, Gary" 11/17/2008 2:14 PM >>> We come in and process them ! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, Michele Sent: Monday, November 17, 2008 9:37 AM To: histonet@pathology.swmed.edu Subject: [Histonet] breast bx processing Hi All, We are trying to figure out how to adjust our processing schedule for breast core bxs on the weekends so that they are not sitting in formalin for too long. Just wondering what everyone else is doing. Your input would be very helpful. Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Mon Nov 17 14:43:40 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Nov 17 14:43:36 2008 Subject: [Histonet] Re: Oil red o for lipid success, clean results Message-ID: <062707EC355F476B88C82F1B0E21AC43@DHXTS541> We have used the Churukian method for oil red O staining of lipids for years. It totally avoids the messy, thick, gooey propylene glycol method and is very clean. It is a simple method, with easy to make up reagents. It can be done on fresh tissue frozen sections fixed immediately after sectioning by immersion fixation in NBF (or paraformaldehyde) OR one can section formalin fixed tissue cryoprotected in 30% sucrose before freezing. After staining, the section can be counterstained with hematoxylin. I will be happy to forward the protocol via private email attachment. It also works very well for lipids in cell cultures. Gayle M. Callis HTL(ASCP)HT,MT From cbobrowi <@t> mcw.edu Mon Nov 17 14:45:13 2008 From: cbobrowi <@t> mcw.edu (Bobrowitz, Carol) Date: Mon Nov 17 14:46:48 2008 Subject: [Histonet] Fibrin stain Message-ID: <8F78639AC56F4143B267FE5F5A1B92C8F0AF48@guyton.phys.mcw.edu> Hello Everyone, I need to do some staining for fibrin. One stain will be the PTAH. I would make up the stain but ripening time I don't have. Any recommendations for a vendor who makes and supplies the PTAH stain solution? Also an acid red 35 stain has been mentioned. I'm pretty sure I've been told the wrong acid red. There are stains for fibrin that use an acid red. Any recommendations would be appreciated? Thank you in advance for your help. Carol Ann Bobrowitz HT/HTL Department of Physiology Medical College of Wisconsin 414-456-8179 cbobrowi@mcw.edu From mtitford <@t> aol.com Mon Nov 17 15:06:34 2008 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Mon Nov 17 15:07:05 2008 Subject: [Histonet] Inspection of research labs / ISO 9000? Message-ID: <8CB170F817ACBAC-724-853@WEBMAIL-MB18.sysops.aol.com> Kimberly Tuttle in Baltimore asks about lab inspections. I attended a workshop at the NSH S/C in Toronto where Diane Sterchi gave a really good workshop entitled, "Cracking the code: regulatory issues in the laboratory".? She spoke about the ISO 9000 certification requirements?that some research laboratories, I think drug company laboratories, must follow. Their requirements are really strict. Much more complicated than CAP.?If?Joe Nocito thinks he has problems now, he had better hope the CAP does not start ?following the ISO?guidelines! Michael Titford Pathology USA Mobile AL From gayle.callis <@t> bresnan.net Mon Nov 17 15:14:59 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Nov 17 15:14:56 2008 Subject: [Histonet] Fibrin stain References: <8F78639AC56F4143B267FE5F5A1B92C8F0AF48@guyton.phys.mcw.edu> Message-ID: Carol, You may want to reconsider using PTAH for fibrin as the fixative of choice is Zenkers or after formalin fixation, a Zenkers postfixation/mordanting, a mercury fixative. I don't think you will want to mess with mercury disposal. One can speed up ripening time of PTAH by adding potassium permangante, but I suggest you use a different mercury free staining method. Peggy Wenk graciously sent me the Martius Scarlet Blue (MSB) after Lendrum some years ago. I would be happy to send that method to you via private email. She gave a lot of details on how to make it work in the method, and it was not that difficult to set up. Gayle M. Callis HTL(ASCP)HT,MT ----- Original Message ----- From: "Bobrowitz, Carol" To: Sent: Monday, November 17, 2008 1:45 PM Subject: [Histonet] Fibrin stain Hello Everyone, I need to do some staining for fibrin. One stain will be the PTAH. I would make up the stain but ripening time I don't have. Any recommendations for a vendor who makes and supplies the PTAH stain solution? Also an acid red 35 stain has been mentioned. I'm pretty sure I've been told the wrong acid red. There are stains for fibrin that use an acid red. Any recommendations would be appreciated? Thank you in advance for your help. Carol Ann Bobrowitz HT/HTL Department of Physiology Medical College of Wisconsin 414-456-8179 cbobrowi@mcw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Nov 17 15:20:59 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 17 15:21:05 2008 Subject: [Histonet] Inspection of research labs / ISO 9000? In-Reply-To: <8CB170F817ACBAC-724-853@WEBMAIL-MB18.sysops.aol.com> Message-ID: <22338.66185.qm@web65702.mail.ac4.yahoo.com> As a matter of fact CAP is going to start offering the ISO certification (as a choice, not as a substitute) in this last quarter of 2008. Ren? J. --- On Mon, 11/17/08, mtitford@aol.com wrote: From: mtitford@aol.com Subject: [Histonet] Inspection of research labs / ISO 9000? To: histonet@lists.utsouthwestern.edu Date: Monday, November 17, 2008, 4:06 PM Kimberly Tuttle in Baltimore asks about lab inspections. I attended a workshop at the NSH S/C in Toronto where Diane Sterchi gave a really good workshop entitled, "Cracking the code: regulatory issues in the laboratory".? She spoke about the ISO 9000 certification requirements?that some research laboratories, I think drug company laboratories, must follow. Their requirements are really strict. Much more complicated than CAP.?If?Joe Nocito thinks he has problems now, he had better hope the CAP does not start ?following the ISO?guidelines! Michael Titford Pathology USA Mobile AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mweirauch <@t> crittenton.com Mon Nov 17 15:32:36 2008 From: mweirauch <@t> crittenton.com (Maray Weirauch) Date: Mon Nov 17 15:33:50 2008 Subject: [Histonet] Error tracking Message-ID: Hey Histonetters, The lab administration at our hospital has asked us to come up with a method to record all errors in Histology, categorize them by type of error/impact of error, and develop a plan of action to reduce/eliminate (haha) errors. I have done a literature search for information regarding error tracking in laboratories and have found some info, mostly relating to clinical pathology. Does anyone have any ideas or information they'd be willing to share with us? Thanks in advance! Maray From victor <@t> pathology.washington.edu Mon Nov 17 15:48:18 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Nov 17 15:48:24 2008 Subject: [Histonet] Error tracking In-Reply-To: References: Message-ID: <4921E6A2.3020503@pathology.washington.edu> In our LIS we have one screen that we can customize what appears. We have created a section for quality control and have various topics that only require the user to check the box. We also have a section for free text. Some topics include block mislabeled, slide mislabeled, requisition incomplete, etc. A monthly report is run compiling the data by problem. For issues outside Pathology, a supervisor will talk with the offenders. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Maray Weirauch wrote: > Hey Histonetters, > > The lab administration at our hospital has asked us to come up with a > method to record all errors in Histology, categorize them by type of > error/impact of error, and develop a plan of action to reduce/eliminate > (haha) errors. > I have done a literature search for information regarding error > tracking in laboratories and have found some info, mostly relating to > clinical pathology. > Does anyone have any ideas or information they'd be willing to share > with us? > > Thanks in advance! > Maray > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AnthonyH <@t> chw.edu.au Mon Nov 17 15:51:23 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Nov 17 15:51:35 2008 Subject: [Histonet] Oil Red O despair In-Reply-To: <62A8156F8071C8439080D626DF8C33A602E554@wave-mail.7thwave.local> Message-ID: One of the main conditions that may prevent an adequate Oil Red O stain is the saturation of the dye. It can take one or more weeks for the Oil Red O dye to reach saturation in the solvent. We have two bottles of Oil Red O solution prepared. We always prepare another batch when the first is emptied so that one is "ripening" while the older solution is being used. (Oh gee - did that make sense?) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Tuesday, 18 November 2008 4:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil Red O despair I'm wondering what are the pros (or cons) of using the propylene glycol version of Oil Red O over the isopropanol method. Is one more suited to a specific application? I'm trying to stain a frozen section of liver, which one would suspect would be loaded with fat, and have thus far been unsuccessful using the propylene glycol Oil Red O. Is there something obvious that I'm missing here? I'm new to cryosectioning...perhaps I did something wrong in the cryostat. I fixed my sections in NBF before staining. Please help. I'm feeling like a pathetic excuse for a histotech. Any advice is greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From gayle.callis <@t> bresnan.net Mon Nov 17 15:52:10 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Nov 17 15:52:13 2008 Subject: [Histonet] Biocare MM HRP or AP polymer kit on frozen sections Message-ID: <6A59433F82754B24ADE0E449DA93E029@DHXTS541> Has anyone used this kit on frozen sections where formalin fixed paraffin embedded tissue is avoided entirely? Biocare only has a data sheet for FFPE tissues, followed by the Decloaker or Decloaker/pepsin digestion protocols. For many of us using frozen sections exclusively with mouse on mouse IHC or IFA applications, some guidelines would be appreciated for frozen sections that are not aldehyde fixed and these MM polymer kits. Hopefully someone has played with this kit on fresh tissue frozen sections? Gayle M. Callis HTL(ASCP)HT,MT Bozeman MT From shive003 <@t> umn.edu Mon Nov 17 15:58:11 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Nov 17 15:58:14 2008 Subject: [Histonet] Error tracking References: Message-ID: <0C6ABECEA7D24C1AA0742FBBA4347C74@auxs.umn.edu> We use a software program called Q-Pulse by Gael, Ltd. Each lab has to record its "nonconformances", select the root causes, identify a corrective action if needed, perform a corrective action if needed, and do a follow-up... all these actions are documented in this software program. Each lab supervisor is able to see what nonconformances are still unresolved/not closed out in their lab, and the program's in-house administrative manager can track problems that seem to be recurring. The Q-Pulse program also enables us to record training records and staff proficiency testing, equipment calibration data, and all of our SOPs. You can find out if this is what you're looking for by going to their website (entering in 'Q-Pulse' in your search terms is more direct). Jan Shivers ----- Original Message ----- From: "Maray Weirauch" To: Sent: Monday, November 17, 2008 3:32 PM Subject: [Histonet] Error tracking > Hey Histonetters, > > The lab administration at our hospital has asked us to come up with a > method to record all errors in Histology, categorize them by type of > error/impact of error, and develop a plan of action to reduce/eliminate > (haha) errors. > I have done a literature search for information regarding error > tracking in laboratories and have found some info, mostly relating to > clinical pathology. > Does anyone have any ideas or information they'd be willing to share > with us? > > Thanks in advance! > Maray > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From adafeldman <@t> anatechltdusa.com Mon Nov 17 16:16:47 2008 From: adafeldman <@t> anatechltdusa.com (Ada Feldman) Date: Mon Nov 17 16:16:51 2008 Subject: [Histonet] Oil Red O despair In-Reply-To: <62A8156F8071C8439080D626DF8C33A602E554@wave-mail.7thwave.local> References: <62A8156F8071C8439080D626DF8C33A602E554@wave-mail.7thwave.local> Message-ID: <55F691DE-4537-4904-83A9-2B0EFB6332BE@anatechltdusa.com> Dear Michele, One of my first histology research papers was "Relative effectiveness of various solvents for oil red O" (Medical Laboratory Technology, 1974, 335-341.) It was concluded that propylene glycol solvents for oil red O produced poor contrasts. Isopropanol, as a solvent, can dissolve tissue lipids. This latter problem is reduced by diluting the isopropanol with water, but dye may precipitate on the tissue section. Our research showed optimal oil red O staining with triethyl phosphate (0.5% oil red O in 60% triethyl phosphate, 5 minutes.) Triethyl phosphate solvent produced dark staining and no precipitate. Send me your mailing address and I will be happy to send you a reprint. Ada Feldman Anatech Ltd 1020 Harts Lake Road Battle Creek, MI 49037 800.262.8324 adafeldman@anatechltdusa.com On Nov 17, 2008, at 12:56 PM, Michele Wich wrote: > I'm wondering what are the pros (or cons) of using the propylene > glycol > version of Oil Red O over the isopropanol method. Is one more > suited to > a specific application? > > I'm trying to stain a frozen section of liver, which one would suspect > would be loaded with fat, and have thus far been unsuccessful using > the > propylene glycol Oil Red O. > > Is there something obvious that I'm missing here? I'm new to > cryosectioning...perhaps I did something wrong in the cryostat. I > fixed > my sections in NBF before staining. > > Please help. I'm feeling like a pathetic excuse for a histotech. Any > advice is greatly appreciated. > > > This communication is intended solely for the use of the addressee > and may contain information that is legally privileged, > confidential or exempt from disclosure. If you are not the > intended recipient, please note that any dissemination, > distribution, or copying of this communication is strictly > prohibited. Anyone who receives this message in error should > notify the sender immediately and delete it from his or her computer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jaustin1967 <@t> gmail.com Mon Nov 17 16:38:15 2008 From: jaustin1967 <@t> gmail.com (Michael Bradley) Date: Mon Nov 17 16:38:21 2008 Subject: [Histonet] Dako Message-ID: Hi all I just heard that Dako has a new Immunostaining system. Is that true? The last new thing from Dako I have heard of was the Eriden and that tanked. Does anyone use the newest Dako system for immunostaining and what do you think of it? Thanks From Rcartun <@t> harthosp.org Mon Nov 17 17:18:38 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Nov 17 17:18:47 2008 Subject: [Histonet] experiences with kappa/lambda In-Reply-To: <4E6C71CA61B34519B5EB7451A0A52813@dielangs.at> References: <4E6C71CA61B34519B5EB7451A0A52813@dielangs.at> Message-ID: <4921B57E0200007700006EA1@gwmail4.harthosp.org> Please refer to our paper, "Immunohistochemical detection of immunoglobulin light chain expression in B-cell non-Hodgkin lymphomas using formalin-fixed, paraffin-embedded tissues and a heat-induced epitope retrieval technique" that appeared in Applied Immunohistochemistry and Molecular Morphology 10(3):258-262, 2002. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Gudrun Lang" 11/12/08 10:44 AM >>> Hi listmembers, Please tell me about your experiences with the IHC-demonstration of kappa and lambda on FFPE-sections. We have some troubles about inconsistend staining and therefore false results about the monoclonality of tumors. Bye Gudrun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Mon Nov 17 17:49:57 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Nov 17 17:50:02 2008 Subject: [Histonet] Oil Red O despair Message-ID: <582736990811171549k64171d9fhd6eb3dcb0ba320d0@mail.gmail.com> Michele, You could try 0.5% Oil Red O in 60% triethylphosphate. That works really well here. I've also used a formulation I found in Humanson, the same amount of Oil Red O in 99% ethanol and it worked fairly well. Triethylphosphate is much cleaner than the ethanol. (There was a precipitate on the slide.) I can't avouch for isopropyl as I used ethanol, but it is good to know there's an alternative in case we run out of triethylphosphate. Amos Brooks Message: 10 Date: Mon, 17 Nov 2008 11:56:45 -0600 From: "Michele Wich" Subject: [Histonet] Oil Red O despair To: Message-ID: <62A8156F8071C8439080D626DF8C33A602E554@wave-mail.7thwave.local> Content-Type: text/plain; charset="us-ascii" I'm wondering what are the pros (or cons) of using the propylene glycol version of Oil Red O over the isopropanol method. Is one more suited to a specific application? I'm trying to stain a frozen section of liver, which one would suspect would be loaded with fat, and have thus far been unsuccessful using the propylene glycol Oil Red O. Is there something obvious that I'm missing here? I'm new to cryosectioning...perhaps I did something wrong in the cryostat. I fixed my sections in NBF before staining. Please help. I'm feeling like a pathetic excuse for a histotech. Any advice is greatly appreciated. From jnocito <@t> satx.rr.com Mon Nov 17 18:56:24 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Nov 17 18:56:31 2008 Subject: [Histonet] JCAHO question References: <938D716CD445614ABBB817517557B6F439DF4573@NADCWPMSGCMS09.hca.corpad.net> Message-ID: <6FC21B929B994ED0841181A15FBEA912@yourxhtr8hvc4p> Jessica, in my experience, CAP trumps JCAHO. Every hospital that I've worked in that the lab was accredited by CAP, JCAHO left the lab alone, except for taking a walk through and maybe asking some safety questions. JTT ----- Original Message ----- From: "Vacca Jessica" To: Sent: Monday, November 17, 2008 1:05 PM Subject: [Histonet] JCAHO question I was presented by my lab manager this question when it comes to F.S. and FNA's, - The key word here is THE ORDERING OF: Does anyone monitor the time in which the specimen is removed from the OR to the time it is called back. Our specimens are delivered to us and clocked in at the time of receipt by the lab to the time in which it is resulted back. In CAP it does not state the "start" in which the TAT is monitored. We are both CAP and JCAHO, Do you have 2 separate policies or do you just use your CAP policy? NPSG.02.03.01 The [organization] measures, assesses, and, if needed, takes action to improve the timeliness of reporting, and the timeliness of receipt of critical tests and critical results and values by the responsible licensed caregiver. Elements of Performance for NPSG.02.03.01 1 The laboratory defines critical tests and critical results and values. 2 The laboratory defines the acceptable length of time between the ordering of critical tests and reporting the results of these tests, whether normal or abnormal. 3 The laboratory defines the acceptable length of time for reporting the results of routine tests with critical abnormal values or findings. 4 The laboratory defines the acceptable length of time between the availability of critical tests and critical results and values and receipt by the responsible licensed caregiver. 5 The laboratory collects data on the timeliness of reporting critical test results and critical results and values from routine tests. 6 The laboratory assesses the data on the timeliness of reporting critical test results and critical results and values from routine tests and determines whether a need for improvement exists. 7 The laboratory takes appropriate action to improve the timeliness of reporting critical test results and critical results and values from routine tests and measures the effectiveness of those actions. 8 Critically abnormal test results are communicated quickly to a responsible licensed caregiver so that prompt action may be taken. 9 When the responsible licensed caregiver is not available, a back-up reporting system provides the information in a timely manner to another qualified responsible caregiver to prevent avoidable delays in treatment or response. Jessica Vacca Histology Supervisor 119 Oakfield Dr Brandon Fl 33511 (813) 571-5193 (813) 571-5169 FAX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Mon Nov 17 20:29:59 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Nov 17 20:30:08 2008 Subject: [Histonet] Fibrin stain In-Reply-To: <8F78639AC56F4143B267FE5F5A1B92C8F0AF48@guyton.phys.mcw.edu> Message-ID: <001d01c94925$8e0687a0$0202a8c0@HPPav2> You can oxidize it with potassium permanganate. It can use used immediately (maybe a half hour of mixing), but works better if allowed to set overnight. It will remain good for about a year. In the School, I like to make up a batch, and have the students oxidize part of it with the potassium permangante, for use that week. We store this in a dark bottle, and it's good for about a year. The other part we put in a clear bottle, and leave on the counter for about 3 months, to allow it to naturally oxidize. After that, we put it in a dark bottle in the cupboard. It stays good for about 3 years. So one batch, split, is good for immediate use and then for longer term use. PHOSPHOTUNGSTIC ACID - HEMATOXYLIN (PTAH) 20.0 g Phosphotungstic acid (P2O524Wo3CxH20) 900 mL Distilled water, hot but not boiling. Heat in microwave. 1.0 g Hematoxylin (CI 75290) 10.0 mL Absolute ethanol, reagent Dissolve together phosphotungstic acid in HOT distilled water. Allow phosphotungstic acid solution to cool. Dissolve together hematoxylin in absolute alcohol. Mix together hematoxylin solution and cooled phosphotungstic solution. The solution ripens naturally in several (3-4) months if allowed to set on the counter in light in a clear bottle. After that, transfer to a dark bottle, and store in the cupboard. Will be stable for at least one year, up to about 3 years. Store at room temperature. Do NOT reuse. (If the stain is needed for immediate use, add 0.177 g potassium permanganate to the solution. When artificially ripened solution is a reddish-brown color, it is ready for use.) To get around the Zenker post-mordant, we found a procedure in Ann Preece that said to post-mordant the slide in Bouins at 60 degree C. for 1 hour. This works much better than the Zenker (more even staining), and we don't have to worry about mercury. Just follow the rest of the procedure after post-mordanting. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bobrowitz, Carol Sent: Monday, November 17, 2008 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fibrin stain Hello Everyone, I need to do some staining for fibrin. One stain will be the PTAH. I would make up the stain but ripening time I don't have. Any recommendations for a vendor who makes and supplies the PTAH stain solution? Also an acid red 35 stain has been mentioned. I'm pretty sure I've been told the wrong acid red. There are stains for fibrin that use an acid red. Any recommendations would be appreciated? Thank you in advance for your help. Carol Ann Bobrowitz HT/HTL Department of Physiology Medical College of Wisconsin 414-456-8179 cbobrowi@mcw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From epoc101 <@t> gmail.com Mon Nov 17 21:33:55 2008 From: epoc101 <@t> gmail.com (Steve) Date: Mon Nov 17 21:34:00 2008 Subject: [Histonet] Old EM microscopes Message-ID: <231dd8040811171933i14149ab0w1996d01b826ded9d@mail.gmail.com> Thanks to all for their reply on my EM post. Your information was very helpful. From pieronelva01 <@t> bigpond.com Tue Nov 18 02:48:58 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Tue Nov 18 02:49:12 2008 Subject: [Histonet] CD4 on Ventana Benchmark References: <938D716CD445614ABBB817517557B6F439DF4573@NADCWPMSGCMS09.hca.corpad.net> <6FC21B929B994ED0841181A15FBEA912@yourxhtr8hvc4p> Message-ID: Dear Histonetters Thanks for all your good advice on CD4. I've finally got a stain I'm happy to look at. For the record, the technique is 4B12 clone, Standard retrieval and 42 minutes incubation, followed by an amplification step. Another big improvment was using a fresh (ie cut at the moment) control, instead of a stock (ie cut about two weeks before). Any thoughts on the use of precut controls in general? Regards Piero Nelva Anatomical Pathology Monash Medical Centre Australia From jqb7 <@t> cdc.gov Tue Nov 18 05:30:43 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Nov 18 05:41:06 2008 Subject: [Histonet] MSB for fibrin Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C4FC@LTA3VS011.ees.hhs.gov> Morning everyone, Does anyone know of a company that sells this procedure in kit form or at least the components? We use the artisan for the majority of our specials and do not have much in the way of reagents available to make the components required for the MSB. Thanks, Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From b-frederick <@t> northwestern.edu Tue Nov 18 08:07:27 2008 From: b-frederick <@t> northwestern.edu (b-frederick@northwestern.edu) Date: Tue Nov 18 08:07:30 2008 Subject: [Histonet] Who inspects research labs? Message-ID: <20081118140727.61CF2745C@merle.it.northwestern.edu> We are inspected by CAP as we are involved with human clinical trials/tissue as well as animal tissue (we cut it for the labs here). We fall under the NIH/NCI and they come visit us too as do our bosses from ECOG (Eastern Cooperative Oncology Group). Pharma companies come and do site visits as we are involved in that too. Our functions are general histo,IHC,FISH,DNA/RNA extraction,tissue banking,clinical trilas and anyhting else you might think of. Fun!!! Bernice Bernice Frederick HTL (ASCP) Robert H. Lurie Cancer Center ECOGPCO-RL Northwestern University Chicago IL 60611 ==============Original message text=============== On Mon, 17 Nov 2008 12:52:00 pm CST "Kimberly Tuttle" wrote: Does anyone know what agency inspects research labs? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ===========End of original message text=========== From b-frederick <@t> northwestern.edu Tue Nov 18 08:18:10 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Nov 18 08:18:25 2008 Subject: [Histonet] fibrin In-Reply-To: <111320082238.16395.491CAC71000A179A0000400B22230704929B0A02D2089B9A019C04040A0DBF04070E000E020A9D@att.net> Message-ID: <009a01c94988$7f12d3d0$d00f7ca5@lurie.northwestern.edu> And Rowley biochemical has the reagents as well as the PTAH for those looking. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of renafail@bellsouth.net Sent: Thursday, November 13, 2008 4:39 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] fibrin The Fraser-Lendrum in the AFIP manual demonstrates fibrin Rena Fail -------------- Original message from "Bartlett, Jeanine (CDC/CCID/NCZVED)" : -------------- > Happy Thursday everyone! > > I would like to know what special stain is being used for fibrin > demonstration. > > Thanks in advance for your help! > > Jeanine Bartlett, BS, HT(ASCP)QIHC > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, MS/G-32 > 18/SB-114 > Atlanta, GA 30333 > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Tue Nov 18 08:47:52 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Nov 18 08:48:11 2008 Subject: [Histonet] breast bx processing In-Reply-To: References: <49218D75.2B7F.00C9.0@geisinger.edu> Message-ID: <49228F48.2B7F.00C9.0@geisinger.edu> Ok, first of all, I mis-typed--they are loaded Saturday afternoon, not Friday. So the tissue may sit in paraffin for 2-4 extra hours. >>> "Jackie M O'Connor" 11/17/2008 3:41 PM >>> How long are they in molten paraffin???? "Angela Bitting" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/17/2008 02:27 PM To "Michele Margiotta" , "Gary Martin" , cc Subject RE: [Histonet] breast bx processing We have staff in on Sunday afternoon, so we start them Friday night and let them sit in paraffin until the techs come in on Sunday. The extra paraffin has actually cut down the number of dry outs we were having. >>> "Martin, Gary" 11/17/2008 2:14 PM >>> We come in and process them ! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, Michele Sent: Monday, November 17, 2008 9:37 AM To: histonet@pathology.swmed.edu Subject: [Histonet] breast bx processing Hi All, We are trying to figure out how to adjust our processing schedule for breast core bxs on the weekends so that they are not sitting in formalin for too long. Just wondering what everyone else is doing. Your input would be very helpful. Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. 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Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SharonC <@t> celligent.net Tue Nov 18 08:54:23 2008 From: SharonC <@t> celligent.net (Sharon Campbell) Date: Tue Nov 18 08:54:28 2008 Subject: [Histonet] Etchable Slides Message-ID: Good morning Histonetters: I am looking for a company that sells Shurmark Plus slides at a reasonable rate or a company that sells an equivalent brand that will work on a Shurmark printer. Thanks for your help. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 (704) 549-8444 x104 sharonc@celligent.net From jqb7 <@t> cdc.gov Tue Nov 18 09:01:24 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Nov 18 09:04:21 2008 Subject: [Histonet] Etchable Slides In-Reply-To: References: Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C50B@LTA3VS011.ees.hhs.gov> TBS http://www.trianglebiomedical.com/SHURMARKslides.cfm Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Campbell Sent: Tuesday, November 18, 2008 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Etchable Slides Good morning Histonetters: I am looking for a company that sells Shurmark Plus slides at a reasonable rate or a company that sells an equivalent brand that will work on a Shurmark printer. Thanks for your help. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 (704) 549-8444 x104 sharonc@celligent.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Tue Nov 18 09:40:44 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Nov 18 09:40:48 2008 Subject: [Histonet] MSB for fibrin References: <1CE1847DFEA0A647B1CCDE4108EA60A70208C4FC@LTA3VS011.ees.hhs.gov> Message-ID: <3ABD039A84414A70BE1ED996169BF1AC@DHXTS541> I know that Poly Scientific will do custom reagent preparation but I am not sure if they have a kit made up for this. We have found their products/kits reliable, and they are very helpful.and pleasant to deal with when we needed something special. Gayle M. Callis HTL(ASCP)HT,MT ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: Sent: Tuesday, November 18, 2008 4:30 AM Subject: [Histonet] MSB for fibrin Morning everyone, Does anyone know of a company that sells this procedure in kit form or at least the components? We use the artisan for the majority of our specials and do not have much in the way of reagents available to make the components required for the MSB. Thanks, Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Emilyf <@t> HEALTHCARESCOUTS.COM Tue Nov 18 09:37:38 2008 From: Emilyf <@t> HEALTHCARESCOUTS.COM (Emily Ferguson) Date: Tue Nov 18 09:45:51 2008 Subject: [Histonet] Histology Positions Nationwide Message-ID: The #1 Recruiter for Laboratory/Biotech Specialists! Hello, My name is Emily Ferguson, I am a National Recruitment and Sales Executive with the Laboratory Division of Healthcare Scouts. We specialize in the direct, permanent placement of medical laboratory & biotech professionals nationwide. I am currently recruiting for Histotechnologist positions in the following areas: * Los Angeles, CA * Fort Wayne, IN * Charlotte, NC * Myrtle Beach, SC * Dallas, TX * San Antonio, TX * Seattle, WA * St Petersburg, FL * Baltimore, MD * Las Vegas, NV * Plattsburgh, NY * Charlottesville, VA * Roanoke, VA Please contact me at the information below. Allow me the opportunity to work on your career! Emily Ferguson National Recruitment & Sales Executive Laboratory & Biotech Division Healthcare Scouts Office: 800-708-0605 ext.132 Cell: 407-529-7274 Fax: 877-478-6064 emilyf@healthcarescouts.com www.healthcarescouts.com From algranth <@t> u.arizona.edu Tue Nov 18 10:12:19 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Nov 18 10:12:11 2008 Subject: [Histonet] Oil Red O despair In-Reply-To: <582736990811171549k64171d9fhd6eb3dcb0ba320d0@mail.gmail.co m> References: <582736990811171549k64171d9fhd6eb3dcb0ba320d0@mail.gmail.com> Message-ID: <6.2.3.4.1.20081118090422.026e60d0@algranth.inbox.email.arizona.edu> I get many projects here that require frozen liver sections and Oil Red O staining. Some years ago I was having problems with the staining and someone on histonet suggested the PolyScientific R & D Oil Red O Stain Kit. I got the kit and have been using it ever since. The stain works great - usually I have livers loaded with lipid - and the slides come out clean. The kit uses proplyene glycol and glycerin jelly to mount the coverslips. I do use one part of the ORO protocol from Freida Carson's book - I fix the slides in 37-40% (page 152, second edition). Andi Grantham At 04:49 PM 11/17/2008, Amos Brooks wrote: >Michele, > You could try 0.5% Oil Red O in 60% triethylphosphate. That works really >well here. I've also used a formulation I found in Humanson, the same amount >of Oil Red O in 99% ethanol and it worked fairly well. Triethylphosphate is >much cleaner than the ethanol. (There was a precipitate on the slide.) I >can't avouch for isopropyl as I used ethanol, but it is good to know there's >an alternative in case we run out of triethylphosphate. > >Amos Brooks > >Message: 10 >Date: Mon, 17 Nov 2008 11:56:45 -0600 >From: "Michele Wich" >Subject: [Histonet] Oil Red O despair >To: >Message-ID: > <62A8156F8071C8439080D626DF8C33A602E554@wave-mail.7thwave.local> >Content-Type: text/plain; charset="us-ascii" > >I'm wondering what are the pros (or cons) of using the propylene glycol >version of Oil Red O over the isopropanol method. Is one more suited to >a specific application? > >I'm trying to stain a frozen section of liver, which one would suspect >would be loaded with fat, and have thus far been unsuccessful using the >propylene glycol Oil Red O. > >Is there something obvious that I'm missing here? I'm new to >cryosectioning...perhaps I did something wrong in the cryostat. I fixed >my sections in NBF before staining. > >Please help. I'm feeling like a pathetic excuse for a histotech. Any >advice is greatly appreciated. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From DixonM <@t> vetmed.ufl.edu Tue Nov 18 10:19:07 2008 From: DixonM <@t> vetmed.ufl.edu (MaryAnn Dixon) Date: Tue Nov 18 10:19:14 2008 Subject: [Histonet] anti-GFP Message-ID: <530D827EC657DE418C3572ADD63FCDC32494EF@EXGVMCNETWORK.vetmed.ufl.edu> Hi histonetters, I stumbled into immunofluorescence for the first time and could use some advice. I am trying to stain GFP on formalin fixed paraffin embedded sections. I have a conjugated alexa fluor 488 anti-gfp antibody from invitrogen that I've now found out was not tested on paraffin sections. I have seen articles supporting and denying that it works. In addition, do I retrieve or not as again, I've seen literature supporting both. Moreover, one article cut sections at 12 microns. My protocol for my first run consisted of a protein block for 10 minutes, blowing off, 1:400 of the conjugated alexa fluor 488 ant-gfp antibody for 1 hour at room temp., buffer rinse, DI water rinse, aqueous mounting medium, and coverslip. To my best ability I performed everything in the dark. The results were that I had no fluorescing whatsoever!! Any help would be appreciated. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 From algranth <@t> u.arizona.edu Tue Nov 18 10:23:16 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Nov 18 10:26:15 2008 Subject: [Histonet] HistoClear question Message-ID: <6.2.3.4.1.20081118091228.02708640@algranth.inbox.email.arizona.edu> A student in my lab is doing some work in another lab here and having some problems with coverslipping their H&E sections. They are using HistoClear 1X as a clearing agent and Cytoseal mounting medium. From looking at the coverslipped slides I'm guessing that these two products are not compatible. The mounting medium is sort of clumping up under coverslips and has a hazy appearance. Yes, I did also find a bit of water in their HistoClear as well. Does anybody else have any experience with HistoClear 1X and/or does anybody know what mounting medium is good to use with the product? I suggested that they call the company who manufactures HistoClear but I don't know if they have done that yet. Thanks, Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From JCBRITTON <@t> Cheshire-Med.COM Tue Nov 18 10:31:37 2008 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Tue Nov 18 10:31:43 2008 Subject: [Histonet] Dako In-Reply-To: References: Message-ID: Hi, We just got the new Bond Max IHC stainer and we love it. You just cut the slides dry them and place them on the Bond. It does retrieval, antibody staining, and counter stain. You just dehydrate , clear, and mount your coverslip. It is easy to use. It has 3 individual slide tray's of 10. You can load more slides on the empty tray's and start a new batch while the others are running. We run into the pathologist's adding more antibodies to the list an hour after we have run the first batch frequently, so this feature is great. When you add more IHC's the run time on all the slide tray's run times do increase, but it's better than having to wait another 2-3 hours to put your next set of immuno's on. Hope this helps! Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From algranth <@t> u.arizona.edu Tue Nov 18 10:35:39 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Nov 18 10:35:30 2008 Subject: [Histonet] Oil Red O despair - point of clarification In-Reply-To: <6.2.3.4.1.20081118090422.026e60d0@algranth.inbox.email.ari zona.edu> References: <582736990811171549k64171d9fhd6eb3dcb0ba320d0@mail.gmail.com> <6.2.3.4.1.20081118090422.026e60d0@algranth.inbox.email.arizona.edu> Message-ID: <6.2.3.4.1.20081118092759.026fceb0@algranth.inbox.email.arizona.edu> I didn't mean that the propylene glycol and glycerin jelly were combined - just wanted to clear that up. The protocol uses both but for different things. Andi Grantham At 09:12 AM 11/18/2008, Andrea Grantham wrote: >I get many projects here that require frozen liver sections and Oil >Red O staining. Some years ago I was having problems with the >staining and someone on histonet suggested the PolyScientific R & D >Oil Red O Stain Kit. I got the kit and have been using it ever >since. The stain works great - usually I have livers loaded with >lipid - and the slides come out clean. The kit uses proplyene glycol >and glycerin jelly to mount the coverslips. I do use one part of the >ORO protocol from Freida Carson's book - I fix the slides in 37-40% >(page 152, second edition). > >Andi Grantham > > > > >At 04:49 PM 11/17/2008, Amos Brooks wrote: >>Michele, >> You could try 0.5% Oil Red O in 60% triethylphosphate. That works really >>well here. I've also used a formulation I found in Humanson, the same amount >>of Oil Red O in 99% ethanol and it worked fairly well. Triethylphosphate is >>much cleaner than the ethanol. (There was a precipitate on the slide.) I >>can't avouch for isopropyl as I used ethanol, but it is good to know there's >>an alternative in case we run out of triethylphosphate. >> >>Amos Brooks >> >>Message: 10 >>Date: Mon, 17 Nov 2008 11:56:45 -0600 >>From: "Michele Wich" >>Subject: [Histonet] Oil Red O despair >>To: >>Message-ID: >> <62A8156F8071C8439080D626DF8C33A602E554@wave-mail.7thwave.local> >>Content-Type: text/plain; charset="us-ascii" >> >>I'm wondering what are the pros (or cons) of using the propylene glycol >>version of Oil Red O over the isopropanol method. Is one more suited to >>a specific application? >> >>I'm trying to stain a frozen section of liver, which one would suspect >>would be loaded with fat, and have thus far been unsuccessful using the >>propylene glycol Oil Red O. >> >>Is there something obvious that I'm missing here? I'm new to >>cryosectioning...perhaps I did something wrong in the cryostat. I fixed >>my sections in NBF before staining. >> >>Please help. I'm feeling like a pathetic excuse for a histotech. Any >>advice is greatly appreciated. >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From cbarone <@t> NEMOURS.ORG Tue Nov 18 10:43:40 2008 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Tue Nov 18 10:43:51 2008 Subject: [Histonet] transfer of slides for proficiency testing for muscle enzyme histochem, IF and IHC...only? Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE8014718EB@wlmmsx01.nemours.org> Looking for someone who is interested in transferring slides, to grade for proficiency on just these...nothing else. Would need to commit to every 6 monsth exchange for CLIA requirement for a consult lab. From talulahgosh <@t> gmail.com Tue Nov 18 10:46:41 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Nov 18 10:46:46 2008 Subject: [Histonet] anti-GFP In-Reply-To: <530D827EC657DE418C3572ADD63FCDC32494EF@EXGVMCNETWORK.vetmed.ufl.edu> References: <530D827EC657DE418C3572ADD63FCDC32494EF@EXGVMCNETWORK.vetmed.ufl.edu> Message-ID: We've tried Invitrogen's rabbit anti-GFP on paraffin sections and it doesn't work with DAB or fluorescent label. Our fix is Carnoy's or butanol, and we cut at 10 microns. With cryosectioning, the antibody works perfect, so it's definitely not the antibody. I'm not sure what in the paraffin processing would destroy the GFP antigen, but apparently something does. Emily On Tue, Nov 18, 2008 at 11:19 AM, MaryAnn Dixon wrote: > Hi histonetters, > > > > I stumbled into immunofluorescence for the first time and could use some > advice. I am trying to stain GFP on formalin fixed paraffin embedded > sections. I have a conjugated alexa fluor 488 anti-gfp antibody from > invitrogen that I've now found out was not tested on paraffin sections. > I have seen articles supporting and denying that it works. In addition, > do I retrieve or not as again, I've seen literature supporting both. > Moreover, one article cut sections at 12 microns. My protocol for my > first run consisted of a protein block for 10 minutes, blowing off, > 1:400 of the conjugated alexa fluor 488 ant-gfp antibody for 1 hour at > room temp., buffer rinse, DI water rinse, aqueous mounting medium, and > coverslip. To my best ability I performed everything in the dark. The > results were that I had no fluorescing whatsoever!! Any help would be > appreciated. > > > > MaryAnn Dixon BS > > Biological Scientist > > Anatomic Pathology > > UF Veterinary Medical Center > > (352) 392-2235 Ext. 4517 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Millions of creatures walk the earth Unseen, both when we wake and when we sleep. -John Milton From jfish <@t> gladstone.ucsf.edu Tue Nov 18 10:58:37 2008 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Tue Nov 18 10:58:51 2008 Subject: [Histonet] HistoClear question In-Reply-To: <6.2.3.4.1.20081118091228.02708640@algranth.inbox.email.arizona.edu> References: <6.2.3.4.1.20081118091228.02708640@algranth.inbox.email.arizona.edu> Message-ID: <2DF13C70CF2C4368BE98382B0A212759@JFISH> Dear Andrea, We stopped using the xylene substitute when my new co-worker started here. She doesn't like change and since she used only xylene at her last job, I compromised and switched back to using the real stuff. You have to choose your battles and since she would have set up additional stations to include the xylene anyway, I gave in. I used to use the mounting medium from ThermoElectron, called Shandon Xylene Substitute Mountant. It was nicely miscible with the histoclear. I don't know the new catalog number since they have merged so many times with other companies. I guess you could search the Fisher Scientific website. Good luck, Jo Dee ~~Jo Dee Fish~~ Senior Research Technologist The J. David Gladstone Institutes Co-manager Histology and Microscopy Core Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Tuesday, November 18, 2008 8:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoClear question A student in my lab is doing some work in another lab here and having some problems with coverslipping their H&E sections. They are using HistoClear 1X as a clearing agent and Cytoseal mounting medium. From looking at the coverslipped slides I'm guessing that these two products are not compatible. The mounting medium is sort of clumping up under coverslips and has a hazy appearance. Yes, I did also find a bit of water in their HistoClear as well. Does anybody else have any experience with HistoClear 1X and/or does anybody know what mounting medium is good to use with the product? I suggested that they call the company who manufactures HistoClear but I don't know if they have done that yet. Thanks, Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Tue Nov 18 11:03:44 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Nov 18 11:03:51 2008 Subject: [Histonet] anti-GFP References: <530D827EC657DE418C3572ADD63FCDC32494EF@EXGVMCNETWORK.vetmed.ufl.edu> Message-ID: The problem may be that you are using a directly conjugated antiGFP rather than a rabbit antiGFP followed by coming back with a secondary conjugated to Alea Fluor 488. This is called quenching, a phenomenom, where fluorophore moleculas too close together on cells or in tissues tend to cancel out the fluorecsing ability of the fluorophore. You should go onto internet and look at a fluorescence Jablonski diagram which show how this occurs, Olympus website also wonderful discussions in pdf form, for all fluorescence applications, including this diagram - for confocal and fluorescent microscopes. I suggest you stain for GFP (Teri Johnson method) where you retrieve, use a rabbit antiGFP, then come back with a secondary either conjugated to FITC (Jackson has excellent antibodies, or one of the Cy fluorophores, or better yet, Goat antirabbit-Alexa 488. Be sure you use Molecular Probes Prolong gold antifade mounting media after staining - this is superior for preventing fading of fluorophores, even 488. Not all aqueous mounting medias will prevent fading of fluorophores, even the Alexa dyes. Teri recommends rabbit antiGFP rather than Goat antiGFP for paraffin work, as the rabbit hosted antibody gave less background than the goat antiGFP. One can also purchase Rabbit antiGFP from Rockland. I made a CC to Teri Johnson so she is in this email loop. You may want to discuss this problem with her, and what antigen recovery method she prefers. If worse comes to worse, and you can't afford another antibody, use the antiGFP-488, come back with an antiAlexa 488 (Molecular Probes) and detect that antibody with an antibody that has FITC, or the appropriate fluorophore. A round about way, but the same type of technic used to detect FITC. Gayle M. Callis HTL(ASCP)HT,MT ----- Original Message ----- From: "MaryAnn Dixon" To: Sent: Tuesday, November 18, 2008 9:19 AM Subject: [Histonet] anti-GFP Hi histonetters, I stumbled into immunofluorescence for the first time and could use some advice. I am trying to stain GFP on formalin fixed paraffin embedded sections. I have a conjugated alexa fluor 488 anti-gfp antibody from invitrogen that I've now found out was not tested on paraffin sections. I have seen articles supporting and denying that it works. In addition, do I retrieve or not as again, I've seen literature supporting both. Moreover, one article cut sections at 12 microns. My protocol for my first run consisted of a protein block for 10 minutes, blowing off, 1:400 of the conjugated alexa fluor 488 ant-gfp antibody for 1 hour at room temp., buffer rinse, DI water rinse, aqueous mounting medium, and coverslip. To my best ability I performed everything in the dark. The results were that I had no fluorescing whatsoever!! Any help would be appreciated. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Tue Nov 18 11:09:13 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Nov 18 11:09:18 2008 Subject: [Histonet] anti-GFP In-Reply-To: References: <530D827EC657DE418C3572ADD63FCDC32494EF@EXGVMCNETWORK.vetmed.ufl.edu> Message-ID: In response to the email below: We use a separate secondary label with our GFP antibody and it doesn't work for us, so that's probably not the problem. Our secondary is from Jackson Immuno, Cy2. Also, we do antigen retrieval with a microwave, which also doesn't help. Emily On Tue, Nov 18, 2008 at 12:03 PM, Gayle Callis wrote: > The problem may be that you are using a directly conjugated antiGFP rather > than a rabbit antiGFP followed by coming back with a secondary conjugated to > Alea Fluor 488. This is called quenching, a phenomenom, where fluorophore > moleculas too close together on cells or in tissues tend to cancel out the > fluorecsing ability of the fluorophore. You should go onto internet and > look at a fluorescence Jablonski diagram which show how this occurs, Olympus > website also wonderful discussions in pdf form, for all fluorescence > applications, including this diagram - for confocal and fluorescent > microscopes. > > I suggest you stain for GFP (Teri Johnson method) where you retrieve, use a > rabbit antiGFP, then come back with a secondary either conjugated to FITC > (Jackson has excellent antibodies, or one of the Cy fluorophores, or better > yet, Goat antirabbit-Alexa 488. Be sure you use Molecular Probes Prolong > gold antifade mounting media after staining - this is superior for > preventing fading of fluorophores, even 488. Not all aqueous mounting > medias will prevent fading of fluorophores, even the Alexa dyes. > > Teri recommends rabbit antiGFP rather than Goat antiGFP for paraffin work, > as the rabbit hosted antibody gave less background than the goat antiGFP. > > One can also purchase Rabbit antiGFP from Rockland. > > I made a CC to Teri Johnson so she is in this email loop. You may want to > discuss this problem with her, and what antigen recovery method she prefers. > > If worse comes to worse, and you can't afford another antibody, use the > antiGFP-488, come back with an antiAlexa 488 (Molecular Probes) and detect > that antibody with an antibody that has FITC, or the appropriate > fluorophore. A round about way, but the same type of technic used to detect > FITC. > > Gayle M. Callis > HTL(ASCP)HT,MT > > -- Millions of creatures walk the earth Unseen, both when we wake and when we sleep. -John Milton From jhnspam <@t> aol.com Tue Nov 18 11:13:51 2008 From: jhnspam <@t> aol.com (pam johnson) Date: Tue Nov 18 11:14:06 2008 Subject: [Histonet] Telly's fixative Message-ID: <8CB17B8294D07B7-A9C-B26@WEBMAIL-MC19.sysops.aol.com> Does anyone have a recipe for Telly's fixative? Thanks, Pam From rjbuesa <@t> yahoo.com Tue Nov 18 11:15:29 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 18 11:15:34 2008 Subject: [Histonet] HistoClear question In-Reply-To: <6.2.3.4.1.20081118091228.02708640@algranth.inbox.email.arizona.edu> Message-ID: <721040.40172.qm@web65703.mail.ac4.yahoo.com> Andi: Histoclear (in all its "variants" and names, like Histolene) is a d-Limonene? containing substance known to having incompatibility problems with mounting media, specially those toluene containing and has to be used with?the proprietary mounting medium brand Histomount that is xylene based. Besides that, Histoclear is notorious for fading hematoxylin, and is not as good as xylene for dewaxing sections before IHC. Has a strong odor and has been reported as hardening brain, liver and spleen. Has caused skin allergic reactions and headaches. Ren? J. --- On Tue, 11/18/08, Andrea Grantham wrote: From: Andrea Grantham Subject: [Histonet] HistoClear question To: histonet@lists.utsouthwestern.edu Date: Tuesday, November 18, 2008, 11:23 AM A student in my lab is doing some work in another lab here and having some problems with coverslipping their H&E sections. They are using HistoClear 1X as a clearing agent and Cytoseal mounting medium. From looking at the coverslipped slides I'm guessing that these two products are not compatible. The mounting medium is sort of clumping up under coverslips and has a hazy appearance. Yes, I did also find a bit of water in their HistoClear as well. Does anybody else have any experience with HistoClear 1X and/or does anybody know what mounting medium is good to use with the product? I suggested that they call the company who manufactures HistoClear but I don't know if they have done that yet. Thanks, Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Tue Nov 18 11:17:17 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Nov 18 11:17:17 2008 Subject: [Histonet] HistoClear question In-Reply-To: <6.2.3.4.1.20081118091228.02708640@algranth.inbox.email.arizona.edu> References: <6.2.3.4.1.20081118091228.02708640@algranth.inbox.email.arizona.edu> Message-ID: <123301B4E6C64AAE80E61BA23EB7AE39@FULLSTAFF.ORG> Hey Andi-- We had the same problem at a derm lab. HistoClear has a companion product for mounting media...it's the only one that works well (is it HistoMount or NeoMount??). Your supplier should have it listed alongside. You'll be AMAZED once you switch--a world of difference and no issues with water even if your HistoClear isn't pristine. Also--watch what alcohols you use right before the HistoClear. Reagent or something other than ethanol seems to work better. Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Tuesday, November 18, 2008 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoClear question A student in my lab is doing some work in another lab here and having some problems with coverslipping their H&E sections. They are using HistoClear 1X as a clearing agent and Cytoseal mounting medium. From looking at the coverslipped slides I'm guessing that these two products are not compatible. The mounting medium is sort of clumping up under coverslips and has a hazy appearance. Yes, I did also find a bit of water in their HistoClear as well. Does anybody else have any experience with HistoClear 1X and/or does anybody know what mounting medium is good to use with the product? I suggested that they call the company who manufactures HistoClear but I don't know if they have done that yet. Thanks, Andi Grantham ...................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Tue Nov 18 11:24:54 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Nov 18 11:25:21 2008 Subject: [Histonet] anti-GFP In-Reply-To: Message-ID: Thanks for the nod Gayle. We've converted to Rockland goat anti-GFP after having some reproducibility issues with the rabbit polyclonal we were using from Novus. I agree with your recommendation to use an indirect IHC method to increase the signal. If she's using a rabbit anti-GFP/Alexa 488 she can still come back with an anti-rabbit Alexa 488 and see if that increases the signal that way. She might also try a biotinylated anti-rabbit, and come back with a Streptavidin Alexa 488. I so do not like FITC, have watched it photobleach as I was viewing it. I will leave it to my IHC Specialist to give the details of her experience with using the Rockland goat antibody. I think it works equally well using antigen retrieval or Proteinase K on formalin fixed paraffin embedded animal tissues. And she's used it both with the goat polymer kit and regular anti-goat Alexa 488. Sharon, care to elaborate on the details of your staining protocols? Teri -----Original Message----- From: Gayle Callis [mailto:gayle.callis@bresnan.net] Sent: Tuesday, November 18, 2008 11:04 AM To: MaryAnn Dixon; histonet@lists.utsouthwestern.edu Cc: Johnson, Teri Subject: Re: [Histonet] anti-GFP The problem may be that you are using a directly conjugated antiGFP rather than a rabbit antiGFP followed by coming back with a secondary conjugated to Alea Fluor 488. This is called quenching, a phenomenom, where fluorophore moleculas too close together on cells or in tissues tend to cancel out the fluorecsing ability of the fluorophore. You should go onto internet and look at a fluorescence Jablonski diagram which show how this occurs, Olympus website also wonderful discussions in pdf form, for all fluorescence applications, including this diagram - for confocal and fluorescent microscopes. I suggest you stain for GFP (Teri Johnson method) where you retrieve, use a rabbit antiGFP, then come back with a secondary either conjugated to FITC (Jackson has excellent antibodies, or one of the Cy fluorophores, or better yet, Goat antirabbit-Alexa 488. Be sure you use Molecular Probes Prolong gold antifade mounting media after staining - this is superior for preventing fading of fluorophores, even 488. Not all aqueous mounting medias will prevent fading of fluorophores, even the Alexa dyes. Teri recommends rabbit antiGFP rather than Goat antiGFP for paraffin work, as the rabbit hosted antibody gave less background than the goat antiGFP. One can also purchase Rabbit antiGFP from Rockland. I made a CC to Teri Johnson so she is in this email loop. You may want to discuss this problem with her, and what antigen recovery method she prefers. If worse comes to worse, and you can't afford another antibody, use the antiGFP-488, come back with an antiAlexa 488 (Molecular Probes) and detect that antibody with an antibody that has FITC, or the appropriate fluorophore. A round about way, but the same type of technic used to detect FITC. Gayle M. Callis HTL(ASCP)HT,MT ----- Original Message ----- From: "MaryAnn Dixon" To: Sent: Tuesday, November 18, 2008 9:19 AM Subject: [Histonet] anti-GFP Hi histonetters, I stumbled into immunofluorescence for the first time and could use some advice. I am trying to stain GFP on formalin fixed paraffin embedded sections. I have a conjugated alexa fluor 488 anti-gfp antibody from invitrogen that I've now found out was not tested on paraffin sections. I have seen articles supporting and denying that it works. In addition, do I retrieve or not as again, I've seen literature supporting both. Moreover, one article cut sections at 12 microns. My protocol for my first run consisted of a protein block for 10 minutes, blowing off, 1:400 of the conjugated alexa fluor 488 ant-gfp antibody for 1 hour at room temp., buffer rinse, DI water rinse, aqueous mounting medium, and coverslip. To my best ability I performed everything in the dark. The results were that I had no fluorescing whatsoever!! Any help would be appreciated. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Tue Nov 18 11:36:53 2008 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Nov 18 11:36:59 2008 Subject: [Histonet] Antibody protocol In-Reply-To: Message-ID: <29BE166A2CF48D459853F8EC57CD37E801A82CB1@EXCHANGECLUSTER.yumaregional.local> Needing some help with the staining protocol for HSA (Hepatocyte Specific Antigen) clone OCH1E5, made by Cell marquee to be ran on the Ventana LT/Benchmark platform.. Would anyone be willing to share protocol information with us, as well as staining methodology. Thank you for the help. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. From LSebree <@t> uwhealth.org Tue Nov 18 11:56:39 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Nov 18 11:56:44 2008 Subject: [Histonet] Antibody protocol In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E801A82CB1@EXCHANGECLUSTER.yumaregional.local> Message-ID: This is our protocol for H S A from Biocare, clone OCHIE5: mild CC1, 1:50 dilution for 24", AB block, Hem II/8". If you don't get good results with your CM antibody, try this one as we get beautiful, strong but clean staining. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Tuesday, November 18, 2008 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody protocol Needing some help with the staining protocol for HSA (Hepatocyte Specific Antigen) clone OCH1E5, made by Cell marquee to be ran on the Ventana LT/Benchmark platform.. Would anyone be willing to share protocol information with us, as well as staining methodology. Thank you for the help. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Tue Nov 18 12:16:39 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Nov 18 12:16:39 2008 Subject: Check out this MSDS exerpt for HistoClear RE: [Histonet] HistoClear question In-Reply-To: <721040.40172.qm@web65703.mail.ac4.yahoo.com> References: <6.2.3.4.1.20081118091228.02708640@algranth.inbox.email.arizona.edu> <721040.40172.qm@web65703.mail.ac4.yahoo.com> Message-ID: Read down to Characteristics-- I want to know who they got to taste this stuff??!!! Cheryl _________ HISTOCLEAR (C10H16) A naturally occurring hydrocarbon found in plants. SYNONYMS 1-methyl-4(1-methylethenyl) cyclohexane p-mentha-1,8-diene, d-limonene, Safsolv, Histolene, dipentene. GENERAL PRECAUTIONS Avoid skin contact, can cause dermatitis. Avoid inhalation. Keep away from heat or naked flames. Wash hands thoroughly after handling. CHARACTERISTICS Clear liquid of low viscosity with a fresh, light and sweet citrusy odour. The taste is sweet, refreshing, citrus-like and mild. From jqb7 <@t> cdc.gov Tue Nov 18 12:20:34 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Nov 18 12:20:57 2008 Subject: Check out this MSDS exerpt for HistoClear RE: [Histonet] HistoClear question In-Reply-To: References: <721040.40172.qm@web65703.mail.ac4.yahoo.com> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208C51A@LTA3VS011.ees.hhs.gov> LOL! That is too funny! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, November 18, 2008 1:17 PM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Andrea Grantham' Subject: Check out this MSDS exerpt for HistoClear RE: [Histonet] HistoClear question Read down to Characteristics-- I want to know who they got to taste this stuff??!!! Cheryl _________ HISTOCLEAR (C10H16) A naturally occurring hydrocarbon found in plants. SYNONYMS 1-methyl-4(1-methylethenyl) cyclohexane p-mentha-1,8-diene, d-limonene, Safsolv, Histolene, dipentene. GENERAL PRECAUTIONS Avoid skin contact, can cause dermatitis. Avoid inhalation. Keep away from heat or naked flames. Wash hands thoroughly after handling. CHARACTERISTICS Clear liquid of low viscosity with a fresh, light and sweet citrusy odour. The taste is sweet, refreshing, citrus-like and mild. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Tue Nov 18 12:30:56 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Nov 18 12:31:38 2008 Subject: [Histonet] old electron microscopes References: Message-ID: <08A0A863637F1349BBFD83A96B27A50A1201B2@uwhis-xchng3.uwhis.hosp.wisc.edu> I wouldn't want to pay the shipping for that! Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jackie M O'Connor Sent: Fri 11/14/2008 11:52 AM To: Joe Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] old electron microscopes Could you imagine these things on Ebay? Joe Sent by: histonet-bounces@lists.utsouthwestern.edu 11/14/2008 11:21 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] old electron microscopes A freind askded me to post this question to the list and see if he could get any guidance. He has two electron microscopes that appear to this untrained eye to be older than the hills, or quite possibly alien artifacts. One is a Siemens unit (power supply is bigger than a fridge). The other, I believe is also a siemens unit, but it appears newer (relatively), than the other. It's a bit smaller and has an orange body to the tube part. He wants to get rid of them and wants to know if they have any value on today's market? I tried the histoauction, but the page did not seem be working. Any help would be appreciated. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SwainFrancesL <@t> uams.edu Tue Nov 18 12:43:24 2008 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Tue Nov 18 12:43:44 2008 Subject: [Histonet] Telly's fixative In-Reply-To: <8CB17B8294D07B7-A9C-B26@WEBMAIL-MC19.sysops.aol.com> References: <8CB17B8294D07B7-A9C-B26@WEBMAIL-MC19.sysops.aol.com> Message-ID: Telly's Fixative 70% EtOH 100.0 mL Glacial Acetic Acid 5.0 mL 37-40% Formaldehyde 10.0 mL Preserves glycogen, lysis rbs's Transfer to 85% EtOH for storage or processing. This recipe is from Humanson's book. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam johnson Sent: Tuesday, November 18, 2008 11:14 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Telly's fixative Does anyone have a recipe for Telly's fixative? Thanks, Pam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jhnspam <@t> aol.com Tue Nov 18 12:45:31 2008 From: jhnspam <@t> aol.com (pam johnson) Date: Tue Nov 18 12:45:54 2008 Subject: [Histonet] Telly's fixative In-Reply-To: References: <8CB17B8294D07B7-A9C-B26@WEBMAIL-MC19.sysops.aol.com> Message-ID: <8CB17C4F7E3A7ED-1128-739@WEBMAIL-MC19.sysops.aol.com> Thank You!! -----Original Message----- From: Swain, Frances L To: pam johnson ; histonet@pathology.swmed.edu Sent: Tue, 18 Nov 2008 12:43 pm Subject: RE: [Histonet] Telly's fixative Telly's Fixative 70% EtOH 100.0 mL Glacial Acetic Acid 5.0 mL 37-40% Formaldehyde 10.0 mL Preserves glycogen, lysis rbs's Transfer to 85% EtOH for storage or processing. This recipe is from Humanson's book. Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pam johnson Sent: Tuesday, November 18, 2008 11:14 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Telly's fixative Does anyone have a recipe for Telly's fixative? Thanks, Pam _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tkngflght <@t> yahoo.com Tue Nov 18 12:57:21 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Nov 18 12:57:22 2008 Subject: [Histonet] Request: simple frozen section procedure? In-Reply-To: References: <29BE166A2CF48D459853F8EC57CD37E801A82CB1@EXCHANGECLUSTER.yumaregional.local> Message-ID: Hey all! Does anyone have a simple frozen section procedure so I don't have to reinvent this wheel?? It's for simple interoperative frozen technique for cancer margins using an older Tissue Tek and OCT. I have the grossing protocol and decontamination procedure...anything you can share is MUCH appreciated! Has the NAACLS format changed in the last two years or so? (The last time I had to start a manual from scratch--time flies!!) Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org From kelly_colpitts <@t> hotmail.com Tue Nov 18 13:05:20 2008 From: kelly_colpitts <@t> hotmail.com (Kelly Colpitts) Date: Tue Nov 18 13:05:25 2008 Subject: [Histonet] Automated Special Stainers Message-ID: Hello Histoland, We are currently running almost all of our special stains on a Dako Artisan. Our contract is up soon and since Dako keeps raising their cost by quite a bit every year and because of a complete lack of customer service on the part of our new rep, we are looking at other specials stainers. I'm just wondering what other labs are using and would you recommend it. Is anyone using the Biogenex i6000? We do have a Ventana Nexus on demo right now but our medical director does not like the way it does the reticulin or the Masson's Trichrome stains. Thanks for your help, Kelly Colpitts, HT (ASCP) _________________________________________________________________ Proud to be a PC? Show the world. Download the ?I?m a PC? Messenger themepack now. hthttp://clk.atdmt.com/MRT/go/119642558/direct/01/ From contact <@t> excaliburpathology.com Tue Nov 18 13:12:40 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Nov 18 13:12:44 2008 Subject: Check out this MSDS exerpt for HistoClear RE: [Histonet] HistoClear question Message-ID: <103172.46924.qm@web1110.biz.mail.sk1.yahoo.com> Reminds me of a T shirt I saw and am going to order: Chemistry It's like cooking. Just don't lick the spoon! ________________________________ From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: tkngflght@yahoo.com; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; Andrea Grantham Sent: Tuesday, November 18, 2008 12:20:34 PM Subject: RE: Check out this MSDS exerpt for HistoClear RE: [Histonet] HistoClear question LOL!? That is too funny! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, November 18, 2008 1:17 PM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Andrea Grantham' Subject: Check out this MSDS exerpt for HistoClear RE: [Histonet] HistoClear question Read down to Characteristics-- I want to know who they got to taste this stuff??!!! Cheryl _________ HISTOCLEAR (C10H16) A naturally occurring hydrocarbon found in plants. SYNONYMS 1-methyl-4(1-methylethenyl) cyclohexane p-mentha-1,8-diene, d-limonene, Safsolv, Histolene, dipentene. GENERAL PRECAUTIONS Avoid skin contact, can cause dermatitis. Avoid inhalation. Keep away from heat or naked flames. Wash hands thoroughly after handling. CHARACTERISTICS Clear liquid of low viscosity with a fresh, light and sweet citrusy odour. The taste is sweet, refreshing, citrus-like and mild. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Tue Nov 18 13:13:36 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Nov 18 13:13:55 2008 Subject: [Histonet] Frozen block storage Message-ID: <2A582E8156B45F468A62D1F1D20AF083489C02@EX-BE08.ohsu.edu> Hello, I have a question, that has risen recently. How long by JAHCO regulation do we have to retain frozen blocks from immunoflourence studies? Thank you Robyn OHSU From RSRICHMOND <@t> aol.com Tue Nov 18 13:20:49 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Nov 18 13:20:54 2008 Subject: [Histonet] Re: Telly's fixative Message-ID: Pam Johnson asked about "Telly's" fixative. Eric Kellar responded to a similar question 9 years ago - it's in the archives. Anybody know how to pronounce Tellyesniczky? (gesundheit) Bob Richmond Samurai Pathologist Knoxville TN ******************************************* Tellyesniczky/Fekete (Telly's) Fixative - [Lillie, 1965] 70%ETOH 100ml Glacial acetic acid 5ml 37-40% formaldehyde conc. 10ml Recommended for the preservation of glycogen. Lyses rbc's. Widely used by botanist's. Transfer to 85% ETOH. Humason,G. (1972) Animal Tissue Techniques. W.H.Freeman and Co., San Francisco. Eric C. Kellar Histology/Immunohistochemistry University of Pittsburgh Medical Center From pjfnefro <@t> duke.edu Tue Nov 18 13:21:48 2008 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Tue Nov 18 13:21:52 2008 Subject: [Histonet] Re: Check out this MSDS exerpt for HistoClear Message-ID: <62328245-D9AB-4215-B818-68FF6A94187C@duke.edu> Paula- Where can I get one of these? -Pat Flannery > Reminds me of a T shirt I saw and am going to order: > > Chemistry > It's like cooking. > > Just don't lick the spoon! From shive003 <@t> umn.edu Tue Nov 18 13:35:00 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Nov 18 13:35:05 2008 Subject: [Histonet] Re: Check out this MSDS exerpt for HistoClear References: <62328245-D9AB-4215-B818-68FF6A94187C@duke.edu> Message-ID: Me, too! Jan Shivers ----- Original Message ----- From: "Pat Flannery" To: Sent: Tuesday, November 18, 2008 1:21 PM Subject: [Histonet] Re: Check out this MSDS exerpt for HistoClear > Paula- > > Where can I get one of these? > > -Pat Flannery > > >> Reminds me of a T shirt I saw and am going to order: >> >> Chemistry >> It's like cooking. >> >> Just don't lick the spoon! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From CIngles <@t> uwhealth.org Tue Nov 18 13:56:46 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Nov 18 13:57:52 2008 Subject: [Histonet] References: <62328245-D9AB-4215-B818-68FF6A94187C@duke.edu> Message-ID: <08A0A863637F1349BBFD83A96B27A50A1201B4@uwhis-xchng3.uwhis.hosp.wisc.edu> Will Steve Coakley please get in contact with me? I have some info for you. Claire From contact <@t> excaliburpathology.com Tue Nov 18 14:19:11 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Nov 18 14:19:15 2008 Subject: Check out this MSDS exerpt for HistoClear RE: [Histonet]HistoClear question Message-ID: <27828.41673.qm@web1113.biz.mail.sk1.yahoo.com> The Chemistry T shirt is in The Wireless Catalog, but I found it on their website too. http://www.thewirelesscatalog.com/wireless/T-Shirts-amp-Sweatshirts_1HA/Item_Chemistry-is-Like-Cooking-Shirts_VF0751G_ps_cti-1HA.html ________________________________ From: "tbritten@aol.com" To: Paula Pierce Sent: Tuesday, November 18, 2008 1:16:23 PM Subject: Re: Check out this MSDS exerpt for HistoClear RE: [Histonet]HistoClear question Ok this is not a technical question....where do I get the t shirt. Thanks tom Sent from my Verizon Wireless BlackBerry -----Original Message----- From: Paula Pierce Date: Tue, 18 Nov 2008 11:12:40 To: Histonet Subject: Re: Check out this MSDS exerpt for HistoClear RE: [Histonet] ??? HistoClear question Reminds me of a T shirt I saw and am going to order: Chemistry It's like cooking. Just don't lick the spoon! ________________________________ From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: tkngflght@yahoo.com; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; Andrea Grantham Sent: Tuesday, November 18, 2008 12:20:34 PM Subject: RE: Check out this MSDS exerpt for HistoClear RE: [Histonet] HistoClear question LOL!? That is too funny! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, November 18, 2008 1:17 PM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Andrea Grantham' Subject: Check out this MSDS exerpt for HistoClear RE: [Histonet] HistoClear question Read down to Characteristics-- I want to know who they got to taste this stuff??!!! Cheryl _________ HISTOCLEAR (C10H16) A naturally occurring hydrocarbon found in plants. SYNONYMS 1-methyl-4(1-methylethenyl) cyclohexane p-mentha-1,8-diene, d-limonene, Safsolv, Histolene, dipentene. GENERAL PRECAUTIONS Avoid skin contact, can cause dermatitis. Avoid inhalation. Keep away from heat or naked flames. Wash hands thoroughly after handling. CHARACTERISTICS Clear liquid of low viscosity with a fresh, light and sweet citrusy odour. The taste is sweet, refreshing, citrus-like and mild. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Nov 18 14:26:35 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Nov 18 14:27:21 2008 Subject: AW: [Histonet] Re: Telly's fixative In-Reply-To: References: Message-ID: <7A16E438386541698ABB309C258A72D4@dielangs.at> It's a Hungarian name. In Austria we have similar names. And you pronounce it: Tell - like "tell" yes - like "yes" nicz - like "bits" with "n" ky - like "key" bye Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Robert Richmond Gesendet: Dienstag, 18. November 2008 20:21 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: Telly's fixative Pam Johnson asked about "Telly's" fixative. Eric Kellar responded to a similar question 9 years ago - it's in the archives. Anybody know how to pronounce Tellyesniczky? (gesundheit) Bob Richmond Samurai Pathologist Knoxville TN ******************************************* Tellyesniczky/Fekete (Telly's) Fixative - [Lillie, 1965] 70%ETOH 100ml Glacial acetic acid 5ml 37-40% formaldehyde conc. 10ml Recommended for the preservation of glycogen. Lyses rbc's. Widely used by botanist's. Transfer to 85% ETOH. Humason,G. (1972) Animal Tissue Techniques. W.H.Freeman and Co., San Francisco. Eric C. Kellar Histology/Immunohistochemistry University of Pittsburgh Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SLB <@t> Stowers-Institute.org Tue Nov 18 14:37:12 2008 From: SLB <@t> Stowers-Institute.org (Beckham, Sharon) Date: Tue Nov 18 14:37:48 2008 Subject: [Histonet] anti-GFP In-Reply-To: Message-ID: I use the Rockland goat GFP #600-101-215 and it works great. I use the GFP at 1:1000, antigen retrieval in citrate buffer, Biocare's goat HRP-polymer kit. The data sheet says to incubate for 10-15 minutes in both the probe and the polymer, but I usually do it for 7 minutes. Cuts back some on background and overstaining. For fluorescence I use the anti-goat Alexa 488. I found the Rockland GFP to be much more reliable than the Novus. Sharon -----Original Message----- From: Johnson, Teri Sent: Tuesday, November 18, 2008 11:25 AM To: 'Gayle Callis'; MaryAnn Dixon; histonet@lists.utsouthwestern.edu Cc: Beckham, Sharon Subject: RE: [Histonet] anti-GFP Thanks for the nod Gayle. We've converted to Rockland goat anti-GFP after having some reproducibility issues with the rabbit polyclonal we were using from Novus. I agree with your recommendation to use an indirect IHC method to increase the signal. If she's using a rabbit anti-GFP/Alexa 488 she can still come back with an anti-rabbit Alexa 488 and see if that increases the signal that way. She might also try a biotinylated anti-rabbit, and come back with a Streptavidin Alexa 488. I so do not like FITC, have watched it photobleach as I was viewing it. I will leave it to my IHC Specialist to give the details of her experience with using the Rockland goat antibody. I think it works equally well using antigen retrieval or Proteinase K on formalin fixed paraffin embedded animal tissues. And she's used it both with the goat polymer kit and regular anti-goat Alexa 488. Sharon, care to elaborate on the details of your staining protocols? Teri -----Original Message----- From: Gayle Callis [mailto:gayle.callis@bresnan.net] Sent: Tuesday, November 18, 2008 11:04 AM To: MaryAnn Dixon; histonet@lists.utsouthwestern.edu Cc: Johnson, Teri Subject: Re: [Histonet] anti-GFP The problem may be that you are using a directly conjugated antiGFP rather than a rabbit antiGFP followed by coming back with a secondary conjugated to Alea Fluor 488. This is called quenching, a phenomenom, where fluorophore moleculas too close together on cells or in tissues tend to cancel out the fluorecsing ability of the fluorophore. You should go onto internet and look at a fluorescence Jablonski diagram which show how this occurs, Olympus website also wonderful discussions in pdf form, for all fluorescence applications, including this diagram - for confocal and fluorescent microscopes. I suggest you stain for GFP (Teri Johnson method) where you retrieve, use a rabbit antiGFP, then come back with a secondary either conjugated to FITC (Jackson has excellent antibodies, or one of the Cy fluorophores, or better yet, Goat antirabbit-Alexa 488. Be sure you use Molecular Probes Prolong gold antifade mounting media after staining - this is superior for preventing fading of fluorophores, even 488. Not all aqueous mounting medias will prevent fading of fluorophores, even the Alexa dyes. Teri recommends rabbit antiGFP rather than Goat antiGFP for paraffin work, as the rabbit hosted antibody gave less background than the goat antiGFP. One can also purchase Rabbit antiGFP from Rockland. I made a CC to Teri Johnson so she is in this email loop. You may want to discuss this problem with her, and what antigen recovery method she prefers. If worse comes to worse, and you can't afford another antibody, use the antiGFP-488, come back with an antiAlexa 488 (Molecular Probes) and detect that antibody with an antibody that has FITC, or the appropriate fluorophore. A round about way, but the same type of technic used to detect FITC. Gayle M. Callis HTL(ASCP)HT,MT ----- Original Message ----- From: "MaryAnn Dixon" To: Sent: Tuesday, November 18, 2008 9:19 AM Subject: [Histonet] anti-GFP Hi histonetters, I stumbled into immunofluorescence for the first time and could use some advice. I am trying to stain GFP on formalin fixed paraffin embedded sections. I have a conjugated alexa fluor 488 anti-gfp antibody from invitrogen that I've now found out was not tested on paraffin sections. I have seen articles supporting and denying that it works. In addition, do I retrieve or not as again, I've seen literature supporting both. Moreover, one article cut sections at 12 microns. My protocol for my first run consisted of a protein block for 10 minutes, blowing off, 1:400 of the conjugated alexa fluor 488 ant-gfp antibody for 1 hour at room temp., buffer rinse, DI water rinse, aqueous mounting medium, and coverslip. To my best ability I performed everything in the dark. The results were that I had no fluorescing whatsoever!! Any help would be appreciated. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Tue Nov 18 15:02:49 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Nov 18 15:02:54 2008 Subject: [Histonet] anti-GFP References: Message-ID: Thanks Teri, We prefer to use Goat antiGFP-biotinylated. This allows us to come back with Molecular Probes Streptavidin-Alexa 488 and eliminates any secondary. Be careful though, endogenous biotin can bite you on this one. One has to use an avidin/biotin block, and we now use Vectors Streptavidin-biotin block, as this has some special considerations that SA binds to some addressins and epithelial cells. This is in the literature. Our protocol is simple, since we do not work with NBF or PFA fixed tissue- only fresh snap frozen tissue sections, and avoid any antigen retrieval. Our solvent fixation ruins GFP fluorescence which means immunostaining for the GFP. We rarely use antiGFP to detect just GFP, but always as a double IFA stain with a murine CD marker or some other marker. Rockland is an excellent source for antibodies against GFP but also for Red Fluorescent Protein (DsRed from Discosoma sp. - hope I spelled that correctly). This can be confusing since one of the chimeras of GFP is also called RFP, from Aqueora jellyfish. Be careful when shopping for these antibodies. I would like to see Sharon's protocol too. Gayle M. Callis HTL(ASCP)HT,MT ----- Original Message ----- From: "Johnson, Teri" To: "'Gayle Callis'" ; "MaryAnn Dixon" ; Cc: "Beckham, Sharon" Sent: Tuesday, November 18, 2008 10:24 AM Subject: RE: [Histonet] anti-GFP Thanks for the nod Gayle. We've converted to Rockland goat anti-GFP after having some reproducibility issues with the rabbit polyclonal we were using from Novus. I agree with your recommendation to use an indirect IHC method to increase the signal. If she's using a rabbit anti-GFP/Alexa 488 she can still come back with an anti-rabbit Alexa 488 and see if that increases the signal that way. She might also try a biotinylated anti-rabbit, and come back with a Streptavidin Alexa 488. I so do not like FITC, have watched it photobleach as I was viewing it. I will leave it to my IHC Specialist to give the details of her experience with using the Rockland goat antibody. I think it works equally well using antigen retrieval or Proteinase K on formalin fixed paraffin embedded animal tissues. And she's used it both with the goat polymer kit and regular anti-goat Alexa 488. Sharon, care to elaborate on the details of your staining protocols? Teri -----Original Message----- From: Gayle Callis [mailto:gayle.callis@bresnan.net] Sent: Tuesday, November 18, 2008 11:04 AM To: MaryAnn Dixon; histonet@lists.utsouthwestern.edu Cc: Johnson, Teri Subject: Re: [Histonet] anti-GFP The problem may be that you are using a directly conjugated antiGFP rather than a rabbit antiGFP followed by coming back with a secondary conjugated to Alea Fluor 488. This is called quenching, a phenomenom, where fluorophore moleculas too close together on cells or in tissues tend to cancel out the fluorecsing ability of the fluorophore. You should go onto internet and look at a fluorescence Jablonski diagram which show how this occurs, Olympus website also wonderful discussions in pdf form, for all fluorescence applications, including this diagram - for confocal and fluorescent microscopes. I suggest you stain for GFP (Teri Johnson method) where you retrieve, use a rabbit antiGFP, then come back with a secondary either conjugated to FITC (Jackson has excellent antibodies, or one of the Cy fluorophores, or better yet, Goat antirabbit-Alexa 488. Be sure you use Molecular Probes Prolong gold antifade mounting media after staining - this is superior for preventing fading of fluorophores, even 488. Not all aqueous mounting medias will prevent fading of fluorophores, even the Alexa dyes. Teri recommends rabbit antiGFP rather than Goat antiGFP for paraffin work, as the rabbit hosted antibody gave less background than the goat antiGFP. One can also purchase Rabbit antiGFP from Rockland. I made a CC to Teri Johnson so she is in this email loop. You may want to discuss this problem with her, and what antigen recovery method she prefers. If worse comes to worse, and you can't afford another antibody, use the antiGFP-488, come back with an antiAlexa 488 (Molecular Probes) and detect that antibody with an antibody that has FITC, or the appropriate fluorophore. A round about way, but the same type of technic used to detect FITC. Gayle M. Callis HTL(ASCP)HT,MT ----- Original Message ----- From: "MaryAnn Dixon" To: Sent: Tuesday, November 18, 2008 9:19 AM Subject: [Histonet] anti-GFP Hi histonetters, I stumbled into immunofluorescence for the first time and could use some advice. I am trying to stain GFP on formalin fixed paraffin embedded sections. I have a conjugated alexa fluor 488 anti-gfp antibody from invitrogen that I've now found out was not tested on paraffin sections. I have seen articles supporting and denying that it works. In addition, do I retrieve or not as again, I've seen literature supporting both. Moreover, one article cut sections at 12 microns. My protocol for my first run consisted of a protein block for 10 minutes, blowing off, 1:400 of the conjugated alexa fluor 488 ant-gfp antibody for 1 hour at room temp., buffer rinse, DI water rinse, aqueous mounting medium, and coverslip. To my best ability I performed everything in the dark. The results were that I had no fluorescing whatsoever!! Any help would be appreciated. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonetalias <@t> gmail.com Tue Nov 18 15:09:51 2008 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Tue Nov 18 15:09:56 2008 Subject: Check out this MSDS exerpt for HistoClear RE: [Histonet] HistoClear question In-Reply-To: References: <6.2.3.4.1.20081118091228.02708640@algranth.inbox.email.arizona.edu> <721040.40172.qm@web65703.mail.ac4.yahoo.com> Message-ID: <4b6c85510811181309s5a5fd7cfg7edbd1c875a9f235@mail.gmail.com> I use it as a mixer! On Tue, Nov 18, 2008 at 1:16 PM, Cheryl wrote: > Read down to Characteristics-- I want to know who they got to taste this > stuff??!!! > > Cheryl > _________ > > HISTOCLEAR (C10H16) A naturally occurring hydrocarbon found in plants. > > SYNONYMS > 1-methyl-4(1-methylethenyl) cyclohexane p-mentha-1,8-diene, d-limonene, > Safsolv, Histolene, dipentene. > GENERAL PRECAUTIONS > Avoid skin contact, can cause dermatitis. > Avoid inhalation. > Keep away from heat or naked flames. > Wash hands thoroughly after handling. > CHARACTERISTICS > Clear liquid of low viscosity with a fresh, light and sweet citrusy odour. > The taste is sweet, refreshing, citrus-like and mild. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- The Unknown HT(ASCP) From talulahgosh <@t> gmail.com Tue Nov 18 15:16:27 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Nov 18 15:16:32 2008 Subject: Teri Johnson's anti-GFP protocol Re: [Histonet] anti-GFP In-Reply-To: <8D0B49AE119E4062988A3A70D2BAD2E2@DHXTS541> References: <530D827EC657DE418C3572ADD63FCDC32494EF@EXGVMCNETWORK.vetmed.ufl.edu> <8D0B49AE119E4062988A3A70D2BAD2E2@DHXTS541> Message-ID: I'm getting really confused--are we still talking about paraffin sections? Anyway, the protocol referenced in the subject says to fix with paraformaldehyde and then paraffin section. I've never tried that--does it go into xylene or formalin when dehydrating? Emily -- Millions of creatures walk the earth Unseen, both when we wake and when we sleep. -John Milton From AnthonyH <@t> chw.edu.au Tue Nov 18 16:15:06 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Nov 18 16:15:15 2008 Subject: [Histonet] Re: Telly's fixative In-Reply-To: Message-ID: NINE years ago. Wow time flys, also "God Bless You" and check the spelling "Tellnoniczky" or "Tellyesnicxky", Oh just give me a beer or Histoclear on the rocks! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, 19 November 2008 6:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Telly's fixative Pam Johnson asked about "Telly's" fixative. Eric Kellar responded to a similar question 9 years ago - it's in the archives. Anybody know how to pronounce Tellyesniczky? (gesundheit) Bob Richmond Samurai Pathologist Knoxville TN ******************************************* Tellyesniczky/Fekete (Telly's) Fixative - [Lillie, 1965] 70%ETOH 100ml Glacial acetic acid 5ml 37-40% formaldehyde conc. 10ml Recommended for the preservation of glycogen. Lyses rbc's. Widely used by botanist's. Transfer to 85% ETOH. Humason,G. (1972) Animal Tissue Techniques. W.H.Freeman and Co., San Francisco. Eric C. Kellar Histology/Immunohistochemistry University of Pittsburgh Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Rcartun <@t> harthosp.org Tue Nov 18 17:16:21 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Nov 18 17:16:30 2008 Subject: [Histonet] C4d question (not CD4) Message-ID: <492306750200007700006F1E@gwmail4.harthosp.org> We have been using an "RUO"-labeled anti-C4d antibody on certain renal transplant cases for the immunohistochemical detection of humoral rejection for the past several years. We have now been asked by our Transplant team to do this test routinely on all transplant kidney biopsies (which is another issue). Is anyone using an "IVD"-labeled antibody for C4d detection by IHC? Although we get excellent results with this antibody on formalin-fixed, paraffin-embedded tissue, I would prefer to be using an "IVD"-labeled antibody (if one exists). Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From jwatson <@t> gnf.org Tue Nov 18 18:25:34 2008 From: jwatson <@t> gnf.org (James Watson) Date: Tue Nov 18 18:25:41 2008 Subject: [Histonet] anti-GFP In-Reply-To: References: <530D827EC657DE418C3572ADD63FCDC32494EF@EXGVMCNETWORK.vetmed.ufl.edu> Message-ID: We use Zamboni's fixative and are able to use paraffin sections. We do use 5% glycerin in our 100% denatured alcohol so that might be protecting the GFP from being damaged during dehydration. jwatson -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, November 18, 2008 8:47 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] anti-GFP We've tried Invitrogen's rabbit anti-GFP on paraffin sections and it doesn't work with DAB or fluorescent label. Our fix is Carnoy's or butanol, and we cut at 10 microns. With cryosectioning, the antibody works perfect, so it's definitely not the antibody. I'm not sure what in the paraffin processing would destroy the GFP antigen, but apparently something does. Emily On Tue, Nov 18, 2008 at 11:19 AM, MaryAnn Dixon wrote: > Hi histonetters, > > > > I stumbled into immunofluorescence for the first time and could use some > advice. I am trying to stain GFP on formalin fixed paraffin embedded > sections. I have a conjugated alexa fluor 488 anti-gfp antibody from > invitrogen that I've now found out was not tested on paraffin sections. > I have seen articles supporting and denying that it works. In addition, > do I retrieve or not as again, I've seen literature supporting both. > Moreover, one article cut sections at 12 microns. My protocol for my > first run consisted of a protein block for 10 minutes, blowing off, > 1:400 of the conjugated alexa fluor 488 ant-gfp antibody for 1 hour at > room temp., buffer rinse, DI water rinse, aqueous mounting medium, and > coverslip. To my best ability I performed everything in the dark. The > results were that I had no fluorescing whatsoever!! Any help would be > appreciated. > > > > MaryAnn Dixon BS > > Biological Scientist > > Anatomic Pathology > > UF Veterinary Medical Center > > (352) 392-2235 Ext. 4517 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Millions of creatures walk the earth Unseen, both when we wake and when we sleep. -John Milton _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Tue Nov 18 19:28:59 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Nov 18 19:29:07 2008 Subject: Check out this MSDS exerpt for HistoClear RE: [Histonet]HistoClear question In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A70208C51A@LTA3VS011.ees.hhs.gov> Message-ID: <002801c949e6$334a68b0$0202a8c0@HPPav2> Actually, d-limonene is used to impart a lemon/orange flavor in lots of candy, drinks (soda), gum, ice cream, gelatin, cakes, etc. So you've been eating it all along! And putting it on your body in orange/lemon scented soap, detergent, creams, lotions, etc. And cleaning with it (think of all the cleaning solutions you have at home that smell like oranges) and car degreasers. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospitals Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, November 18, 2008 1:21 PM To: tkngflght@yahoo.com; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: RE: Check out this MSDS exerpt for HistoClear RE: [Histonet]HistoClear question LOL! That is too funny! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, November 18, 2008 1:17 PM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Andrea Grantham' Subject: Check out this MSDS exerpt for HistoClear RE: [Histonet] HistoClear question Read down to Characteristics-- I want to know who they got to taste this stuff??!!! Cheryl _________ HISTOCLEAR (C10H16) A naturally occurring hydrocarbon found in plants. SYNONYMS 1-methyl-4(1-methylethenyl) cyclohexane p-mentha-1,8-diene, d-limonene, Safsolv, Histolene, dipentene. GENERAL PRECAUTIONS Avoid skin contact, can cause dermatitis. Avoid inhalation. Keep away from heat or naked flames. Wash hands thoroughly after handling. CHARACTERISTICS Clear liquid of low viscosity with a fresh, light and sweet citrusy odour. The taste is sweet, refreshing, citrus-like and mild. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From freckles9660 <@t> yahoo.com Tue Nov 18 19:33:32 2008 From: freckles9660 <@t> yahoo.com (Karla Arrington) Date: Tue Nov 18 19:33:36 2008 Subject: [Histonet] RE: Uneven H&E Staining Message-ID: <794364.62211.qm@web32507.mail.mud.yahoo.com> Recently we have had an issue with uneven H&E staining.? Lately slides that have three sections on one slide, mostly GI biopsies, and the last section (bottom of slide) are paler than the upper two section.? All cut by the same person and the stains were great.? What could be the cause of this?? Also with the quality of formalin.? Can formalin be purchased as "bad" when it takes 6 days to fix placenta or breasts?? What is the cause? ? Karla Arrington, HT(ASCP) From RSRICHMOND <@t> aol.com Tue Nov 18 19:52:50 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Nov 18 19:52:55 2008 Subject: [Histonet] Re: Telly's fixative Message-ID: I thought Gudrun Lang would know how to pronounce Tellyesniczky if anybody did! But one more question - which syllable is accented? Gudrun wrote: It's a Hungarian name. In Austria we have similar names. And you pronounce it: Tell - like "tell" yes - like "yes" nicz - like "bits" with "n" ky - like "key" Bob Richmond Samurai Pathologist Knoxville TN From AnthonyH <@t> chw.edu.au Tue Nov 18 20:18:18 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Nov 18 20:18:27 2008 Subject: [Histonet] RE: Uneven H&E Staining In-Reply-To: <794364.62211.qm@web32507.mail.mud.yahoo.com> Message-ID: For the pale H&E, has the wax been completely removed in the xylene. If slides are dried upright, then the wax flows down the slide possibly covering the last section, requiring longer in the dewax solution. Secondly, after the differentiation stage, the acid/alcohol again may be collecting at the bottom of the slide especially in the automatic stainers prior to water rinsing (increasing the differentiation time for the lowest section). To differentiate these two causes, is the eosin pale as well? If it is then it might be caused by the incomplete de-waxing. For the long NBF fixation of placenta, has excess blood been removed from the placenta? Or have you checked the concentration of formalin (see Jaspers (1987) J Histotechnol 10 (4): 263-265 for the method using Schiffs reagent). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karla Arrington Sent: Wednesday, 19 November 2008 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Uneven H&E Staining Recently we have had an issue with uneven H&E staining.? Lately slides that have three sections on one slide, mostly GI biopsies, and the last section (bottom of slide) are paler than the upper two section.? All cut by the same person and the stains were great.? What could be the cause of this?? Also with the quality of formalin.? Can formalin be purchased as "bad" when it takes 6 days to fix placenta or breasts?? What is the cause? ? Karla Arrington, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From JMacDonald <@t> mtsac.edu Tue Nov 18 23:30:03 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Nov 18 23:30:02 2008 Subject: [Histonet] Request: simple frozen section procedure? In-Reply-To: Message-ID: Try this website: http://www.pathologyinnovations.com/frozen_section_technique.htm. NCCLS is now known as CLSI. You can check it out at www.clsi.org. The most recent standard for preparing procedures is GP02-A5 (5th edition) 03/14/06 Jennifer MacDonald "Cheryl" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/18/2008 10:59 AM Please respond to tkngflght@yahoo.com To cc Subject [Histonet] Request: simple frozen section procedure? Hey all! Does anyone have a simple frozen section procedure so I don't have to reinvent this wheel?? It's for simple interoperative frozen technique for cancer margins using an older Tissue Tek and OCT. I have the grossing protocol and decontamination procedure...anything you can share is MUCH appreciated! Has the NAACLS format changed in the last two years or so? (The last time I had to start a manual from scratch--time flies!!) Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jessgrocki <@t> yahoo.com Wed Nov 19 04:47:15 2008 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Wed Nov 19 04:47:20 2008 Subject: [Histonet] Isopropyl Message-ID: <889944.81875.qm@web82004.mail.mud.yahoo.com> Good Morning, we are interested in replacing xylene in our lab. Is anyone using graded isopropyl alcohols in there processors? instead of xylene and if so how is it working for you? Thanks. ? Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital From jessgrocki <@t> yahoo.com Wed Nov 19 04:47:15 2008 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Wed Nov 19 04:47:23 2008 Subject: [Histonet] Isopropyl Message-ID: <889944.81875.qm@web82004.mail.mud.yahoo.com> Good Morning, we are interested in replacing xylene in our lab. Is anyone using graded isopropyl alcohols in there processors? instead of xylene and if so how is it working for you? Thanks. ? Jessica Piche-Grocki, HT(ASCP) Waterbury Hospital From Shirley.Chu <@t> moldev.com Wed Nov 19 07:32:01 2008 From: Shirley.Chu <@t> moldev.com (Chu, Shirley) Date: Wed Nov 19 07:33:06 2008 Subject: [Histonet] Laser Capture Microdissection Webinar Message-ID: Announcing MDS Analytical Technologies next in the series of Laser Capture Microdissection and Microgenomics Webinars. Our next LCM webinar will be held on Monday, November 24, 2008 at 10AM PST or 1PM EST. The speaker will be Charmain Pietersen, PhD., Laboratory for Structural and Molecular Neuroscience, McLean Hospital. Her presentation is titled: "Gene expression analysis in homogenous single cell populations in the superior temporal gyrus in postmortem brains from subjects with schizophrenia". This webinar is free to all registrants. For further information and/or to register for this webinar, please connect to the following website: http://www.moleculardevices.com/pages/lcm_webinar_11.2008.html Shirley Chu Application Scientist, Arcturus LCM Products Molecular Devices (now a part of MDS Analytical Technologies) 408-747-3765 | www.moleculardevices.com From mickie25 <@t> netzero.net Wed Nov 19 07:59:12 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Nov 19 07:59:35 2008 Subject: Check out this MSDS exerpt for HistoClear RE: [Histonet]HistoClear question In-Reply-To: <002801c949e6$334a68b0$0202a8c0@HPPav2> References: <1CE1847DFEA0A647B1CCDE4108EA60A70208C51A@LTA3VS011.ees.hhs.gov> <002801c949e6$334a68b0$0202a8c0@HPPav2> Message-ID: And... How many of us have developed sensitivity to d-limonene? I for one have a sensitivity to it and more and more Mohs labs are forgoing fume hoods because it is considered 'safe'. ? Mickie ? Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC ? & Mohs Lab Staffing & Mohs Suite Mohs Reporting Software 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX?? 509-624-3926 Web: www.mohshistogyconsulting.com?& www.mohslabstaffing.com & www.mohssuite.com Email: mickie25@netzero.net ? DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. Thank You. ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Tuesday, November 18, 2008 5:29 PM To: 'Bartlett, Jeanine (CDC/CCID/NCZVED)'; tkngflght@yahoo.com; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Andrea Grantham' Subject: RE: Check out this MSDS exerpt for HistoClear RE: [Histonet]HistoClear question Actually, d-limonene is used to impart a lemon/orange flavor in lots of candy, drinks (soda), gum, ice cream, gelatin, cakes, etc. So you've been eating it all along! And putting it on your body in orange/lemon scented soap, detergent, creams, lotions, etc. And cleaning with it (think of all the cleaning solutions you have at home that smell like oranges) and car degreasers. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospitals Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, November 18, 2008 1:21 PM To: tkngflght@yahoo.com; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: RE: Check out this MSDS exerpt for HistoClear RE: [Histonet]HistoClear question LOL! That is too funny! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, November 18, 2008 1:17 PM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Andrea Grantham' Subject: Check out this MSDS exerpt for HistoClear RE: [Histonet] HistoClear question Read down to Characteristics-- I want to know who they got to taste this stuff??!!! Cheryl _________ HISTOCLEAR (C10H16) A naturally occurring hydrocarbon found in plants. SYNONYMS 1-methyl-4(1-methylethenyl) cyclohexane p-mentha-1,8-diene, d-limonene, Safsolv, Histolene, dipentene. GENERAL PRECAUTIONS Avoid skin contact, can cause dermatitis. Avoid inhalation. Keep away from heat or naked flames. Wash hands thoroughly after handling. CHARACTERISTICS Clear liquid of low viscosity with a fresh, light and sweet citrusy odour. The taste is sweet, refreshing, citrus-like and mild. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.9.4/1794 - Release Date: 11/17/2008 8:48 AM From integrated.histo <@t> gmail.com Wed Nov 19 08:06:22 2008 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Wed Nov 19 08:06:27 2008 Subject: [Histonet] Used Equipment Message-ID: <5d9104a30811190606hc257f54hb6fd01d29ef18cdf@mail.gmail.com> I am searching for a used tissue processor in good condition. Please contact me by e-mail: integrated.histo@gmail.com Cindy DuBois Integrated Pathology Stockton, Ca From jrobertson <@t> pathologysciences.com Wed Nov 19 08:34:06 2008 From: jrobertson <@t> pathologysciences.com (Jodie Robertson) Date: Wed Nov 19 08:34:13 2008 Subject: [Histonet] C4d question (not CD4) In-Reply-To: <492306750200007700006F1E@gwmail4.harthosp.org> Message-ID: <518CD6920AA7154193CBE5977CD88073177D75@psmgsrv2.PSMG.local> Biocare has an IVD predilute that is very good. (PM153AA) We use it on our Benchmark XT. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Chico, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, November 18, 2008 3:16 PM To: Histonet Subject: [Histonet] C4d question (not CD4) We have been using an "RUO"-labeled anti-C4d antibody on certain renal transplant cases for the immunohistochemical detection of humoral rejection for the past several years. We have now been asked by our Transplant team to do this test routinely on all transplant kidney biopsies (which is another issue). Is anyone using an "IVD"-labeled antibody for C4d detection by IHC? Although we get excellent results with this antibody on formalin-fixed, paraffin-embedded tissue, I would prefer to be using an "IVD"-labeled antibody (if one exists). Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Nov 19 08:44:52 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Nov 19 08:45:12 2008 Subject: AW: [Histonet] Re: Telly's fixative In-Reply-To: References: Message-ID: <1519F58D0A6C424489593F0D130E3BB0@dielangs.at> You have to stress the first and the third syllable. Like "sun-shine-rag-gy" "Tell-yes-nicz-ky" Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Robert Richmond Gesendet: Mittwoch, 19. November 2008 02:53 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: Telly's fixative I thought Gudrun Lang would know how to pronounce Tellyesniczky if anybody did! But one more question - which syllable is accented? Gudrun wrote: It's a Hungarian name. In Austria we have similar names. And you pronounce it: Tell - like "tell" yes - like "yes" nicz - like "bits" with "n" ky - like "key" Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Wed Nov 19 08:48:25 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Nov 19 08:48:44 2008 Subject: AW: [Histonet] RE: Uneven H&E Staining In-Reply-To: <794364.62211.qm@web32507.mail.mud.yahoo.com> References: <794364.62211.qm@web32507.mail.mud.yahoo.com> Message-ID: <05F51EAC246B4F10BD8395E750B697DC@dielangs.at> Are the coplin jars all equally filled? If the wash-jars are partly filled, the lower slides would be more differentiated than the upper. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Karla Arrington Gesendet: Mittwoch, 19. November 2008 02:34 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: Uneven H&E Staining Recently we have had an issue with uneven H&E staining.? Lately slides that have three sections on one slide, mostly GI biopsies, and the last section (bottom of slide) are paler than the upper two section.? All cut by the same person and the stains were great.? What could be the cause of this?? Also with the quality of formalin.? Can formalin be purchased as "bad" when it takes 6 days to fix placenta or breasts?? What is the cause? ? Karla Arrington, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Wed Nov 19 08:48:20 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Nov 19 08:48:52 2008 Subject: [Histonet] C4d question (not CD4) In-Reply-To: <518CD6920AA7154193CBE5977CD88073177D75@psmgsrv2.PSMG.local> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21B6451D5@chi2k3ms01.columbuschildrens.net> Jodie, That's for CD4 not C4d Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jodie Robertson Sent: Wednesday, November 19, 2008 9:34 AM To: Richard Cartun; Histonet Subject: RE: [Histonet] C4d question (not CD4) Biocare has an IVD predilute that is very good. (PM153AA) We use it on our Benchmark XT. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Chico, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, November 18, 2008 3:16 PM To: Histonet Subject: [Histonet] C4d question (not CD4) We have been using an "RUO"-labeled anti-C4d antibody on certain renal transplant cases for the immunohistochemical detection of humoral rejection for the past several years. We have now been asked by our Transplant team to do this test routinely on all transplant kidney biopsies (which is another issue). Is anyone using an "IVD"-labeled antibody for C4d detection by IHC? Although we get excellent results with this antibody on formalin-fixed, paraffin-embedded tissue, I would prefer to be using an "IVD"-labeled antibody (if one exists). Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From gu.lang <@t> gmx.at Wed Nov 19 08:51:47 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Nov 19 08:52:11 2008 Subject: AW: [Histonet] C4d question (not CD4) In-Reply-To: <518CD6920AA7154193CBE5977CD88073177D75@psmgsrv2.PSMG.local> References: <492306750200007700006F1E@gwmail4.harthosp.org> <518CD6920AA7154193CBE5977CD88073177D75@psmgsrv2.PSMG.local> Message-ID: <317A576F9E2647CCADB9DA6656B283F0@dielangs.at> This biocare-antibody is CD4 and not complement4d. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jodie Robertson Gesendet: Mittwoch, 19. November 2008 15:34 An: Richard Cartun; Histonet Betreff: RE: [Histonet] C4d question (not CD4) Biocare has an IVD predilute that is very good. (PM153AA) We use it on our Benchmark XT. Jodie Robertson, HT (ASCP) QIHC Pathology Sciences Medical Group Chico, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, November 18, 2008 3:16 PM To: Histonet Subject: [Histonet] C4d question (not CD4) We have been using an "RUO"-labeled anti-C4d antibody on certain renal transplant cases for the immunohistochemical detection of humoral rejection for the past several years. We have now been asked by our Transplant team to do this test routinely on all transplant kidney biopsies (which is another issue). Is anyone using an "IVD"-labeled antibody for C4d detection by IHC? Although we get excellent results with this antibody on formalin-fixed, paraffin-embedded tissue, I would prefer to be using an "IVD"-labeled antibody (if one exists). Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Weber2 <@t> va.gov Wed Nov 19 09:54:20 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Wed Nov 19 09:54:33 2008 Subject: [Histonet] Antibody protocol In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E801A82CB1@EXCHANGECLUSTER.yumaregional.local> References: <29BE166A2CF48D459853F8EC57CD37E801A82CB1@EXCHANGECLUSTER.yumaregional.local> Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76E86@VHAV10MSGA1.v10.med.va.gov> We are using the ultraView DAB from Ventana and the same HSA from Cell Marque. Here is the protocol we are using. Depar as usual; CC1 Standard; HSA incubation at 37C for 32 min; Counterstain Heme 4 min; Post Counterstain Bluing for 4 min. we have found it to be a reliable stain and very clean and crisp. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Tuesday, November 18, 2008 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody protocol Needing some help with the staining protocol for HSA (Hepatocyte Specific Antigen) clone OCH1E5, made by Cell marquee to be ran on the Ventana LT/Benchmark platform.. Would anyone be willing to share protocol information with us, as well as staining methodology. Thank you for the help. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Weber2 <@t> va.gov Wed Nov 19 09:55:59 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Wed Nov 19 09:56:07 2008 Subject: [Histonet] Antibody protocol In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E801A82CB1@EXCHANGECLUSTER.yumaregional.local> References: <29BE166A2CF48D459853F8EC57CD37E801A82CB1@EXCHANGECLUSTER.yumaregional.local> Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76E87@VHAV10MSGA1.v10.med.va.gov> We are using the Benchmark XT. Sorry I omitted that. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Tuesday, November 18, 2008 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody protocol Needing some help with the staining protocol for HSA (Hepatocyte Specific Antigen) clone OCH1E5, made by Cell marquee to be ran on the Ventana LT/Benchmark platform.. Would anyone be willing to share protocol information with us, as well as staining methodology. Thank you for the help. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jellin@yumaregional.org This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Nov 19 11:04:46 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Nov 19 11:04:51 2008 Subject: [Histonet] Telly's fixative. Also Bodian. Message-ID: R. Tellyesniczky published more than one fixative, 1898-190 AFA or FAA: mostly ethyl alcoh and about 5% acetic acid. It was Bodian (Anat. Rec. 69: 153-162) as the best fixative for subsequent (Anat. Rec< 89-97). AFA mixtures are gene formaldehyde fixatives if you need to stain no axons in paraffin sections with any silver method.
 
Bob Richmond may remember Bodian from Johns H functional in people wit virus-killed  muscles that were paralysed b adult human spinal cord is 45 cm columns can be appreciated only in transv
 
John Kiernan
Neuroanat UWO
London, Canada
  http://instruct .uwo.ca/anatomy/530/
= = =
----- Original Messag <jhnspam@aol.com>< November 18, 2008 12:14
Subject: [ Telly's fixative
To: histonet@pathology.swmed .edu

>
> Does anyone have a recip Thanks,< _____________ _______________________ 5F list> htt p://lists.utsouthwestern.edu/mailman/listinfo/histonet
=3 CB From godsgalnow <@t> aol.com Wed Nov 19 12:15:02 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Nov 19 12:15:40 2008 Subject: [Histonet] ThermoWave users Message-ID: <8CB1889E0376F3A-5CC-8BD@WEBMAIL-MB09.sysops.aol.com> All ThermoWave Microwave Processing users.... Would any of you be filling to share your needle cor biopsy SOP (i.e. prostate) and the GI SOP? Thanks Roxanne From JCBRITTON <@t> Cheshire-Med.COM Wed Nov 19 12:51:18 2008 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Wed Nov 19 12:51:27 2008 Subject: [Histonet] RE: Uneven H&E Staining In-Reply-To: <05F51EAC246B4F10BD8395E750B697DC@dielangs.at> References: <794364.62211.qm@web32507.mail.mud.yahoo.com> <05F51EAC246B4F10BD8395E750B697DC@dielangs.at> Message-ID: Sounds to me that your having a de-paraffining problem. Check your xylene or clearing agent, maybe there is water in them or they are not as clean as they should be. Are you on a daily rotation/change of staining/de-paraffin reagents? Maybe, your oven isn't hot enough to melt some paraffin off the slides before de-par! Josie Britton HT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Wednesday, November 19, 2008 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: Uneven H&E Staining Are the coplin jars all equally filled? If the wash-jars are partly filled, the lower slides would be more differentiated than the upper. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Karla Arrington Gesendet: Mittwoch, 19. November 2008 02:34 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: Uneven H&E Staining Recently we have had an issue with uneven H&E staining. Lately slides that have three sections on one slide, mostly GI biopsies, and the last section (bottom of slide) are paler than the upper two section. All cut by the same person and the stains were great. What could be the cause of this? Also with the quality of formalin. Can formalin be purchased as "bad" when it takes 6 days to fix placenta or breasts? What is the cause? Karla Arrington, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From Samuel_Perry <@t> DFCI.HARVARD.EDU Wed Nov 19 13:22:57 2008 From: Samuel_Perry <@t> DFCI.HARVARD.EDU (Perry, Samuel) Date: Wed Nov 19 13:23:08 2008 Subject: [Histonet] therapeutic antibody detection in mouse xenografts? In-Reply-To: References: Message-ID: Hi All, I am wondering if anyone has experience showing therapeutic monoclonal antibody binding in mouse xenografts (human tumor cells injected subcutaneously in SCID mice and then dose treated with therapeutic antibody injected intraperitoneally). For instance, can trastuzamab (Herceptin) binding be seen in a breast tumor xenograft treated with Herceptin? I have tried using Dako's goat-anti mouse dextran HRP conjugated secondary antibody developed with DAB. I also tried using an Abcam goat-anti mouse antibody conjugated with biotin, then adding Vectors elite ABC, then developing with DAB. Both times I used FFPE tissue. In both techniques, results showed minimal to staining and showed no difference between mice treated with antibody and control mice treated. I would appreciate if anyone knew of published papers or have experience using IHC to show therapeutic antibody bound to tumor cell in mouse xenografts. Thanks -Sam Research Technician Dana Farber Cancer Institute Boston MA. The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From jkiernan <@t> uwo.ca Wed Nov 19 13:29:06 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Nov 19 13:29:11 2008 Subject: [Histonet] Re: Buffer. In-Reply-To: References: Message-ID: PIPES-buffered glutaraldehyde has been used for subsequent electron micr artifacts - - when used for perfu Schultz RL Membrane alterations in cerebral cortex when J. Neurocytol. 1 461-469. The authors attributed these art which potentially could be mistaken for pathological changes, the low toxicity of PIPES. Apparently cacodylate or phosphate buff er prevented the metabolic changes that caused the artifacts during the occur. < buffer rather tha calcium ions. A Ca s immobilization of phospholipids in cell membranes.
 
John Kiernan
Anatomy, UW Messa <RSRICHMOND@aol.com& November 16, 2008 14:13
Subjec Buffer.
To: histonet@lists.utso uthwestern.edu

> About the potential toxicity of
> >>The the Microscopy Listserve usually because someone is eith exposure
> or has sa the stuff. It was used in
&g phosphate buffers can interact with some < the
> fixative combinations and leave pre Also, I'd suspect,
> cacodylate is less beasties growing in stock solutions over
> time. "modern" zwitterionic buffers are used a lot these
&g t; days -
> PIPES & that crowd - if you want to phosphate.<< buffers are often referred to as Goo or
> Good buffers, since Norman Good in the 1960's.
> S http://en.wikipedia.org/wiki/Good's_buffers
>
> I don't know why they aren't commo fixatives.
Richmond
> Samurai Pathologi Knoxville TN
>
> ___ _______________________ 5F ____________________
=2 6g Histonet@lists.utsouthwes http://lists.utsouthwestern.edu/mailman From Vickroy.Jim <@t> mhsil.com Wed Nov 19 13:49:12 2008 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Nov 19 13:51:10 2008 Subject: [Histonet] SPECIAL STAINER Message-ID: <24A4826E8EF0964D86BC5317306F58A52BA58FA2A3@mmc-mail.ad.mhsil.com> We have used an automated special stainer for years and are now experiencing multiple problems. Therefore before we order from the same vendor I wondered what everyone else was using for silver stains such as Jones, Retic, and GMS. Just trying to evaluate if replacing instrument with newer model is going to be a positive step or should we look at someone else. thanks Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy.jim@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Sharon.Genest <@t> saskatoonhealthregion.ca Wed Nov 19 15:15:25 2008 From: Sharon.Genest <@t> saskatoonhealthregion.ca (Genest, Sharon SktnHR) Date: Wed Nov 19 15:16:35 2008 Subject: [Histonet] Processing problems Message-ID: We had a problem with our recycler and xylene contamination so we now test each batch by adding water to a sample of recycled alcohol. If it turns cloudy we assumed that we had xylene contamination. We have lately tested commercial ethanols (no turbidity)before placing them on the processor and after we processing with them just once we have turbidity after adding a water to all the ethanols 70-100%. This is happening to all the alcohols on all of our processors. I am thinking that it is not a xylene contamination but something else maybe a leaching of plastic from the cassettes? Has this happened to anyone else? Can anyone give me any suggestions. Sharon From Cindy.J.Chard-Bergstrom <@t> aphis.usda.gov Wed Nov 19 15:31:15 2008 From: Cindy.J.Chard-Bergstrom <@t> aphis.usda.gov (Cindy.J.Chard-Bergstrom@aphis.usda.gov) Date: Wed Nov 19 15:31:20 2008 Subject: [Histonet] Control slide storage Message-ID: The issue was brought up in the lab regarding the storing of IHC control slides. What experience has anyone had on the effects of long term storage and staining quality. We store the slides non preheated (with the paraffin still on them). Cindy J Chard-Bergstrom From CIngles <@t> uwhealth.org Wed Nov 19 15:56:48 2008 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Wed Nov 19 16:00:11 2008 Subject: [Histonet] equipment maintenance References: <24A4826E8EF0964D86BC5317306F58A52BA58FA2A3@mmc-mail.ad.mhsil.com> Message-ID: <08A0A863637F1349BBFD83A96B27A50A1201B8@uwhis-xchng3.uwhis.hosp.wisc.edu> Hey gang, and vendors: We have recently lost our cryostat repairman. Does anyone know of a company that will repair a Microm HM 505? All it needs is a fan motor, but we would like some possible preventative maintenance done as well. Plus someone to contact in case we need repairs in the future. Thanks! Claire From godsgalnow <@t> aol.com Wed Nov 19 16:17:52 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Nov 19 16:18:09 2008 Subject: [Histonet] Dako In-Reply-To: References: Message-ID: <8CB18ABCBFD2E54-638-D4@WEBMAIL-MC11.sysops.aol.com> Just another FYI... The Biocare IntelliPath does the same thing, but has 4 trays of 10 and it allows you to make one of the trays?RUSH.? SO depending, on the volume?of IHC you do either of these machines would work out great. Roxanne -----Original Message----- From: Josie Britton To: Michael Bradley ; Histonet@lists.utsouthwestern.edu Sent: Tue, 18 Nov 2008 11:31 am Subject: RE: [Histonet] Dako Hi, We just got the new Bond Max IHC stainer and we love it. You just cut the slides dry them and place them on the Bond. It does retrieval, antibody staining, and counter stain. You just dehydrate , clear, and mount your coverslip. It is easy to use. It has 3 individual slide tray's of 10. You can load more slides on the empty tray's and start a new batch while the others are running. We run into the pathologist's adding more antibodies to the list an hour after we have run the first batch frequently, so this feature is great. When you add more IHC's the run time on all the slide tray's run times do increase, but it's better than having to wait another 2-3 hours to put your next set of immuno's on. Hope this helps! Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Wed Nov 19 16:27:10 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Nov 19 16:27:33 2008 Subject: [Histonet] Dako In-Reply-To: <8CB18ABCBFD2E54-638-D4@WEBMAIL-MC11.sysops.aol.com> References: <8CB18ABCBFD2E54-638-D4@WEBMAIL-MC11.sysops.aol.com> Message-ID: <8CB18AD18F8D06E-638-164@WEBMAIL-MC11.sysops.aol.com> I am sorry---it is 5 trays of 10 including the RUSH tray Roxanne -----Original Message----- From: godsgalnow@aol.com To: JCBRITTON@Cheshire-Med.COM; jaustin1967@gmail.com; Histonet@lists.utsouthwestern.edu Sent: Wed, 19 Nov 2008 5:17 pm Subject: Re: [Histonet] Dako Just another FYI... The Biocare IntelliPath does the same thing, but has 4 trays of 10 and it allows you to make one of the trays?RUSH.? SO depending, on the volume?of IHC you do either of these machines would work out great. Roxanne -----Original Message----- From: Josie Britton To: Michael Bradley ; Histonet@lists.utsouthwestern.edu Sent: Tue, 18 Nov 2008 11:31 am Subject: RE: [Histonet] Dako Hi, We just got the new Bond Max IHC stainer and we love it. You just cut the slides dry them and place them on the Bond. It does retrieval, antibody staining, and counter stain. You just dehydrate , clear, and mount your coverslip. It is easy to use. It has 3 individual slide tray's of 10. You can load more slides on the empty tray's and start a new batch while the others are running. We run into the pathologist's adding more antibodies to the list an hour after we have run the first batch frequently, so this feature is great. When you add more IHC's the run time on all the slide tray's run times do increase, but it's better than having to wait another 2-3 hours to put your next set of immuno's on. Hope this helps! Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tissuetech <@t> juno.com Wed Nov 19 16:25:21 2008 From: tissuetech <@t> juno.com (tissuetech@juno.com) Date: Wed Nov 19 16:27:37 2008 Subject: [Histonet] equipment maintenance Message-ID: <20081119.162521.21375.0@webmail11.vgs.untd.com> Hi Claire Try this company, Microscope & Mictorome Service Company. I use them and they are very good. Fred Siena - Tissue Techniques Pathology Labs ____________________________________________________________ Click to consolidate debt and lower month expenses. http://thirdpartyoffers.juno.com/TGL2141/fc/PnY6rw2PBHhUPXLfAg1a9hw70cTqssbeSKA8zMLpGeD0s5HrKgchz/ From trathborne <@t> somerset-healthcare.com Wed Nov 19 16:30:48 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Nov 19 16:30:55 2008 Subject: [Histonet] Dako In-Reply-To: <8CB18ABCBFD2E54-638-D4@WEBMAIL-MC11.sysops.aol.com> Message-ID: I didn't think that it had deparaffinization and retrieval on line. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of godsgalnow@aol.com Sent: Wednesday, November 19, 2008 5:18 PM To: JCBRITTON@Cheshire-Med.COM; jaustin1967@gmail.com; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako Just another FYI... The Biocare IntelliPath does the same thing, but has 4 trays of 10 and it allows you to make one of the trays?RUSH.? SO depending, on the volume?of IHC you do either of these machines would work out great. Roxanne -----Original Message----- From: Josie Britton To: Michael Bradley ; Histonet@lists.utsouthwestern.edu Sent: Tue, 18 Nov 2008 11:31 am Subject: RE: [Histonet] Dako Hi, We just got the new Bond Max IHC stainer and we love it. You just cut the slides dry them and place them on the Bond. It does retrieval, antibody staining, and counter stain. You just dehydrate , clear, and mount your coverslip. It is easy to use. It has 3 individual slide tray's of 10. You can load more slides on the empty tray's and start a new batch while the others are running. We run into the pathologist's adding more antibodies to the list an hour after we have run the first batch frequently, so this feature is great. When you add more IHC's the run time on all the slide tray's run times do increase, but it's better than having to wait another 2-3 hours to put your next set of immuno's on. Hope this helps! Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From tylerliebig <@t> gmail.com Wed Nov 19 19:17:25 2008 From: tylerliebig <@t> gmail.com (Tyler Liebig) Date: Wed Nov 19 19:17:29 2008 Subject: [Histonet] PSlim Slide Printer Message-ID: Hello Everyone, I have been following Victor's updates on the PSlim slide printer the past couple of months. Who else is using this device out there? I have some questions about it. What kind of slides are you using? Have you had any issues with it? Thanks in advance, Tyler Liebig tylerliebig@gmail.com From RSalcedo <@t> tmhs.org Wed Nov 19 19:31:00 2008 From: RSalcedo <@t> tmhs.org (Salcedo, Rudy) Date: Wed Nov 19 19:33:26 2008 Subject: [Histonet] RE: Histonet Digest, Vol 60, Issue 32 References: <20081119180153.F30021CC8072@mail25-dub.bigfish.com> Message-ID: <0DE2321B1716814E9DF3B6753171E722011336AB@EXCH9.tmh.tmhs> Histonet, anybody know where I can find Sheep Liver, we have a research person looking for a small piece or a block. Thanks in advance. Rudy rsalcedo@tmhs.org Methodist. Leading Medicine. Ranked No. 10 on FORTUNE magazine's list of the "100 Best Companies to Work For" in 2008 Named by U.S. News & World Report as one of "America's Best Hospitals" Designated as a Magnet hospital for excellence in nursing ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. From sccrshlly <@t> yahoo.com Wed Nov 19 21:10:07 2008 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Wed Nov 19 21:10:09 2008 Subject: [Histonet] RE: Uneven H&E Staining (Karla Arrington) Message-ID: <636504.35159.qm@web90301.mail.mud.yahoo.com> One other possiblity to consider is a thinner section at the bottom.? That could be the answer if the problem is intermittent even within the same staining rack.? If the section is thinner, the staining appears paler. ? Good luck discovering the cause! From conniegrubaugh <@t> hotmail.com Wed Nov 19 21:41:28 2008 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Wed Nov 19 21:41:31 2008 Subject: [Histonet] Uneven H & E Stain Message-ID: We had the same problem. The xylene had nothing to do with it and thick and thin sections was one thing that we thought might be the problem. But when I destained and restained the same slides they stained the way they were supposed to . Finally we found that we had a problem with the wash stations of our sakura stainer. We had someone from sakaura come out and work on the machine and the staining has returned to what it is supposed to be. It seems when we have too many racks on the stainer this problem comes back intermitently, and sometimes we have to shut the machine down and turn it back on. Not sure exactly why this happens but if we follow this course of action our slides are staining correctly. Connie Grubaugh Las Vegas Nv. _________________________________________________________________ See how Windows? connects the people, information, and fun that are part of your life http://clk.atdmt.com/MRT/go/119463819/direct/01/ From jnocito <@t> satx.rr.com Thu Nov 20 05:32:18 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Nov 20 05:32:21 2008 Subject: [Histonet] Processing problems References: Message-ID: <6BC5FA687D4B4537BED481549A01B619@yourxhtr8hvc4p> it might be your processor. Depending on the type of processor, some processors have a rotary valve that rotates from reagent bottle to reagent bottle. When the rotary valves fails, it starts placing a reagent in the reagents bottles indiscriminately. For instance, our processor started spitting paraffin in our alcohols. JTT ----- Original Message ----- From: "Genest, Sharon SktnHR" To: Sent: Wednesday, November 19, 2008 3:15 PM Subject: [Histonet] Processing problems We had a problem with our recycler and xylene contamination so we now test each batch by adding water to a sample of recycled alcohol. If it turns cloudy we assumed that we had xylene contamination. We have lately tested commercial ethanols (no turbidity)before placing them on the processor and after we processing with them just once we have turbidity after adding a water to all the ethanols 70-100%. This is happening to all the alcohols on all of our processors. I am thinking that it is not a xylene contamination but something else maybe a leaching of plastic from the cassettes? Has this happened to anyone else? Can anyone give me any suggestions. Sharon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sara.Lees <@t> sanofi-aventis.com Thu Nov 20 06:16:06 2008 From: Sara.Lees <@t> sanofi-aventis.com (Sara.Lees@sanofi-aventis.com) Date: Thu Nov 20 06:16:12 2008 Subject: [Histonet] Leica slide writer - problems?? Message-ID: <4F528252682D36478D2EA05FBECC2DE701944A7C@ALPW31.f2.enterprise> Hi, Has anyone else been having problems with there Lieca slide writer. Our problems mainly involve the ink writing rubbing off before and after staining!!, we use Surgipath snowcoated slides, what does everyone else use as they have hinted it could be our slides. Any help would be fantastic. Thanks Loads Sara From BMolinari <@t> heart.thi.tmc.edu Thu Nov 20 06:43:31 2008 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Nov 20 06:43:35 2008 Subject: [Histonet] RE: Histonet Digest, Vol 60, Issue 32 In-Reply-To: <0DE2321B1716814E9DF3B6753171E722011336AB@EXCH9.tmh.tmhs> References: <20081119180153.F30021CC8072@mail25-dub.bigfish.com> <0DE2321B1716814E9DF3B6753171E722011336AB@EXCH9.tmh.tmhs> Message-ID: Hi Rudy, I may have some. I will look around. Does it have to be fresh, unfrozen? Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Avenue Houston, TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salcedo, Rudy Sent: Wednesday, November 19, 2008 7:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 60, Issue 32 Histonet, anybody know where I can find Sheep Liver, we have a research person looking for a small piece or a block. Thanks in advance. Rudy rsalcedo@tmhs.org Methodist. Leading Medicine. Ranked No. 10 on FORTUNE magazine's list of the "100 Best Companies to Work For" in 2008 Named by U.S. News & World Report as one of "America's Best Hospitals" Designated as a Magnet hospital for excellence in nursing ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. From pruegg <@t> ihctech.net Thu Nov 20 08:09:24 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Nov 20 08:09:25 2008 Subject: [Histonet] Control slide storage In-Reply-To: Message-ID: I store all cut paraffin sections for IHC control at 4dc and without heating to melt the paraffin, even with that I have seen some loss of antigenicity over long periods of time for certain antibodies. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy.J.Chard-Bergstrom@aphis.usda.gov Sent: Wednesday, November 19, 2008 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control slide storage The issue was brought up in the lab regarding the storing of IHC control slides. What experience has anyone had on the effects of long term storage and staining quality. We store the slides non preheated (with the paraffin still on them). Cindy J Chard-Bergstrom _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCBRITTON <@t> Cheshire-Med.COM Thu Nov 20 08:34:34 2008 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Thu Nov 20 08:34:44 2008 Subject: [Histonet] Dako In-Reply-To: References: <8CB18ABCBFD2E54-638-D4@WEBMAIL-MC11.sysops.aol.com> Message-ID: It really does deparaffinize and retrieve. We have been using it since June. That was a big seller for us and did I say we love it? -----Original Message----- From: Rathborne, Toni [mailto:trathborne@somerset-healthcare.com] Sent: Wednesday, November 19, 2008 5:31 PM To: godsgalnow@aol.com; Josie Britton; jaustin1967@gmail.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako I didn't think that it had deparaffinization and retrieval on line. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of godsgalnow@aol.com Sent: Wednesday, November 19, 2008 5:18 PM To: JCBRITTON@Cheshire-Med.COM; jaustin1967@gmail.com; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako Just another FYI... The Biocare IntelliPath does the same thing, but has 4 trays of 10 and it allows you to make one of the trays?RUSH.? SO depending, on the volume?of IHC you do either of these machines would work out great. Roxanne -----Original Message----- From: Josie Britton To: Michael Bradley ; Histonet@lists.utsouthwestern.edu Sent: Tue, 18 Nov 2008 11:31 am Subject: RE: [Histonet] Dako Hi, We just got the new Bond Max IHC stainer and we love it. You just cut the slides dry them and place them on the Bond. It does retrieval, antibody staining, and counter stain. You just dehydrate , clear, and mount your coverslip. It is easy to use. It has 3 individual slide tray's of 10. You can load more slides on the empty tray's and start a new batch while the others are running. We run into the pathologist's adding more antibodies to the list an hour after we have run the first batch frequently, so this feature is great. When you add more IHC's the run time on all the slide tray's run times do increase, but it's better than having to wait another 2-3 hours to put your next set of immuno's on. Hope this helps! Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From mari.ann.mailhiot <@t> leica-microsystems.com Thu Nov 20 08:59:02 2008 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Thu Nov 20 08:59:11 2008 Subject: [Histonet] Leica slide writer - problems?? In-Reply-To: <4F528252682D36478D2EA05FBECC2DE701944A7C@ALPW31.f2.enterprise> Message-ID: Sara Can you give me a call here at Leica and we can discuss. Best regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com Sent by: To histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] Leica slide writer - 11/20/2008 06:16 problems?? AM Hi, Has anyone else been having problems with there Lieca slide writer. Our problems mainly involve the ink writing rubbing off before and after staining!!, we use Surgipath snowcoated slides, what does everyone else use as they have hinted it could be our slides. Any help would be fantastic. Thanks Loads Sara _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From gu.lang <@t> gmx.at Thu Nov 20 09:17:25 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Nov 20 09:17:47 2008 Subject: AW: [Histonet] Control slide storage In-Reply-To: <20081120141403.20674gmx1@mx103.gmx.net> References: <20081120141403.20674gmx1@mx103.gmx.net> Message-ID: <46AAF0DCDC8E465F87AB0F21303EBCF8@dielangs.at> Hi Patsy, I am interested in the names of this certain epitopes, that loose stainability after long time storage. And I'd like to ask all the other listmembers about their experiences with this issue. Perhaps we can make a list of "dangerous" epitopes. Regards Gudrun Lang Akh Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Patsy Ruegg Gesendet: Donnerstag, 20. November 2008 15:09 An: Cindy.J.Chard-Bergstrom@aphis.usda.gov; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Control slide storage I store all cut paraffin sections for IHC control at 4dc and without heating to melt the paraffin, even with that I have seen some loss of antigenicity over long periods of time for certain antibodies. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy.J.Chard-Bergstrom@aphis.usda.gov Sent: Wednesday, November 19, 2008 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control slide storage The issue was brought up in the lab regarding the storing of IHC control slides. What experience has anyone had on the effects of long term storage and staining quality. We store the slides non preheated (with the paraffin still on them). Cindy J Chard-Bergstrom _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Thu Nov 20 09:46:32 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Nov 20 09:46:55 2008 Subject: AW: [Histonet] Control slide storage In-Reply-To: <46AAF0DCDC8E465F87AB0F21303EBCF8@dielangs.at> Message-ID: CD31 for sure. I do recall most surface markers I used tended to be unstable. Jackie O' "Gudrun Lang" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/20/2008 09:17 AM Please respond to gu.lang@gmx.at To cc Subject AW: [Histonet] Control slide storage Hi Patsy, I am interested in the names of this certain epitopes, that loose stainability after long time storage. And I'd like to ask all the other listmembers about their experiences with this issue. Perhaps we can make a list of "dangerous" epitopes. Regards Gudrun Lang Akh Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Patsy Ruegg Gesendet: Donnerstag, 20. November 2008 15:09 An: Cindy.J.Chard-Bergstrom@aphis.usda.gov; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Control slide storage I store all cut paraffin sections for IHC control at 4dc and without heating to melt the paraffin, even with that I have seen some loss of antigenicity over long periods of time for certain antibodies. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy.J.Chard-Bergstrom@aphis.usda.gov Sent: Wednesday, November 19, 2008 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control slide storage The issue was brought up in the lab regarding the storing of IHC control slides. What experience has anyone had on the effects of long term storage and staining quality. We store the slides non preheated (with the paraffin still on them). Cindy J Chard-Bergstrom _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Thu Nov 20 10:34:21 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Nov 20 10:34:39 2008 Subject: AW: [Histonet] Control slide storage In-Reply-To: References: <46AAF0DCDC8E465F87AB0F21303EBCF8@dielangs.at> Message-ID: <7722595275A4DD4FA225B92CDBF174A1744E1B461A@EXC-MBX3.cfs.le.ac.uk> Is it quite as simple as that, I imagine that many epitopes might lose "stainability" if fixation is inadequate or processing poorly controlled. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: 20 November 2008 15:47 To: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Control slide storage CD31 for sure. I do recall most surface markers I used tended to be unstable. Jackie O' "Gudrun Lang" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/20/2008 09:17 AM Please respond to gu.lang@gmx.at To cc Subject AW: [Histonet] Control slide storage Hi Patsy, I am interested in the names of this certain epitopes, that loose stainability after long time storage. And I'd like to ask all the other listmembers about their experiences with this issue. Perhaps we can make a list of "dangerous" epitopes. Regards Gudrun Lang Akh Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Patsy Ruegg Gesendet: Donnerstag, 20. November 2008 15:09 An: Cindy.J.Chard-Bergstrom@aphis.usda.gov; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Control slide storage I store all cut paraffin sections for IHC control at 4dc and without heating to melt the paraffin, even with that I have seen some loss of antigenicity over long periods of time for certain antibodies. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy.J.Chard-Bergstrom@aphis.usda.gov Sent: Wednesday, November 19, 2008 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control slide storage The issue was brought up in the lab regarding the storing of IHC control slides. What experience has anyone had on the effects of long term storage and staining quality. We store the slides non preheated (with the paraffin still on them). Cindy J Chard-Bergstrom _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thomas.crowell <@t> novartis.com Thu Nov 20 10:36:45 2008 From: thomas.crowell <@t> novartis.com (thomas.crowell@novartis.com) Date: Thu Nov 20 10:36:54 2008 Subject: [Histonet] Stain for Cellulose In-Reply-To: <200811201548.mAKFmoIM001492@ch1ssaenov02.novartis.com> Message-ID: I am looking for a stain that will specifically label cellulose, mixed in with other mammalian tisssue elements. Any ideas? Tom Crowell Novartis Institute for BiomedicalReseach Cambridge, MA From gayle.callis <@t> bresnan.net Thu Nov 20 10:46:42 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Nov 20 10:46:45 2008 Subject: [Histonet] Re: Sheep liver References: <20081119180153.F30021CC8072@mail25-dub.bigfish.com><0DE2321B1716814E9DF3B6753171E722011336AB@EXCH9.tmh.tmhs> Message-ID: <725338C3F5014D43A37B3C43D3315992@DHXTS541> If you have a veterinary diagnostic laboratory close by or if they are looking in, they should have some sheep liver, hopefully not autolyzed. I am not sure if local slaughterhouses do sheep, but you can call them to find out. Good Luck Gayle M. Callis HTL(ASCP)HT,MT ----- Original Message ----- From: "Molinari, Betsy" To: Sent: Thursday, November 20, 2008 5:43 AM Subject: RE: [Histonet] RE: Histonet Digest, Vol 60, Issue 32 Hi Rudy, I may have some. I will look around. Does it have to be fresh, unfrozen? Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Avenue Houston, TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salcedo, Rudy Sent: Wednesday, November 19, 2008 7:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 60, Issue 32 Histonet, anybody know where I can find Sheep Liver, we have a research person looking for a small piece or a block. Thanks in advance. Rudy rsalcedo@tmhs.org Methodist. Leading Medicine. Ranked No. 10 on FORTUNE magazine's list of the "100 Best Companies to Work For" in 2008 Named by U.S. News & World Report as one of "America's Best Hospitals" Designated as a Magnet hospital for excellence in nursing ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Nov 20 10:50:40 2008 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Nov 20 10:52:36 2008 Subject: [Histonet] Kappa & Lambda In situ Probes Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB0028AE9C0@e2k3ms1.urmc-sh.rochester.edu> We were using the Kappa and Lambda ISH probes from Dako along with their PNA kit which made them visible under light microscope. Now they have discontinued the Kappa and Lambda probe. Does anyone out there have an alternative that is visible under light microscopy. Thanks Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 From KKay <@t> chr.ab.ca Thu Nov 20 10:56:07 2008 From: KKay <@t> chr.ab.ca (Kay, Karen) Date: Thu Nov 20 10:56:12 2008 Subject: [Histonet] RE: Histonet Digest, Vol 60, Issue 33 RE LEICA SLIDE WRITER In-Reply-To: Message-ID: <9C0BD812BAB0BA4DB7E7FD4F5FA3CAA80A494D84@exbe.chr.ab.ca> Hi Sara, The problem may not be with Leica's Slide Writer but with your Surgipath slides. We too have been experiencing issues with our slides which are Surgipath's. Our printer is the Sakura Slide Writer. We have been told here that Surgipath has changed the formalation/manufacturing of the white paint that these slides are "painted" with. Surgipath "Europe" has apparently made some changes and the problem is being corrected. Unfortunately, right now it's always a ???when we receive a new batch of slides from our Stores area. Our Surgipath Rep and Surgipath Canada have been very good about replacing the faulty slides but it has been a challenge in the midst of our day to day pressures. I would speak with your Surgipath Rep about this issue Hope this information is helpful to you. Karen J Kay, MLT Pathology Supervisor Chinook Health Laboratory Chinook Regional Hospital 960 - 19 st South Lethbridge, Alberta, CANADA T1J 1W5 (403) 388 - 6061 (Phone) (403) 388 - 6067 (Fax) This communication is intended for the use of the recipient to which it is addressed, and may contain confidential, personal and or privileged information. Please contact us immediately if you are not the intended recipient. Do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. From JWeems <@t> sjha.org Thu Nov 20 10:57:28 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Nov 20 10:57:34 2008 Subject: [Histonet] Kappa & Lambda In situ Probes In-Reply-To: <2CF6F6B05263EA4EBAB07781B51E5DB0028AE9C0@e2k3ms1.urmc-sh.rochester.edu> References: <2CF6F6B05263EA4EBAB07781B51E5DB0028AE9C0@e2k3ms1.urmc-sh.rochester.edu> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA516991C@ITSSSXM01V6.one.ads.che.org> Check with Leica (Lab Vision). They knew about the federal changes are were prepared to handle the change. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Thursday, November 20, 2008 11:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kappa & Lambda In situ Probes We were using the Kappa and Lambda ISH probes from Dako along with their PNA kit which made them visible under light microscope. Now they have discontinued the Kappa and Lambda probe. Does anyone out there have an alternative that is visible under light microscopy. Thanks Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From juditw <@t> u.washington.edu Thu Nov 20 11:22:33 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Thu Nov 20 11:22:45 2008 Subject: [Histonet] Leica slide writer - problems?? In-Reply-To: <4F528252682D36478D2EA05FBECC2DE701944A7C@ALPW31.f2.enterprise> Message-ID: Hi all- we have a Leica and I have tried different slides. here is my take on what works and doesn't: >Starfrost Plus or non-plus (German) don't work too well- the pink frosted slides especially - the ink wipes off- the green is better (why?), the white is ok. >Unifrost, non charged, by Ever Scientific (VWR) works well >Super Up-Rite by Richard Allen, non-charged - terrible! bleeds and you can't read it >Colormark Plus by Thermo Sci. for labeling instruments - works Beautifully - this is the one I will go with in future orders! Judy in Washington On Thu, 20 Nov 2008 Sara.Lees@sanofi-aventis.com wrote: > Hi, > > Has anyone else been having problems with there Lieca slide writer. Our > problems mainly involve the ink writing rubbing off before and after > staining!!, we use Surgipath snowcoated slides, what does everyone else > use as they have hinted it could be our slides. Any help would be > fantastic. > > Thanks Loads > > Sara > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From tim.morken <@t> thermofisher.com Thu Nov 20 11:37:44 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Thu Nov 20 11:38:27 2008 Subject: [Histonet] Kappa & Lambda In situ Probes In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA516991C@ITSSSXM01V6.one.ads.che.org> Message-ID: Just a note, Leica is not affiliated with Lab Vision, but is affiliated with Vision Biosystems. Lab Vision/Thermo Fisher Scientific does not carry lambda or kappa ISH probes. Tim Morken Technical Support Manager Immunohistochemistry Anatomical Pathology Thermo Fisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, November 20, 2008 8:57 AM To: McMahon, Loralee A; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kappa & Lambda In situ Probes Check with Leica (Lab Vision). They knew about the federal changes are were prepared to handle the change. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Thursday, November 20, 2008 11:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kappa & Lambda In situ Probes We were using the Kappa and Lambda ISH probes from Dako along with their PNA kit which made them visible under light microscope. Now they have discontinued the Kappa and Lambda probe. Does anyone out there have an alternative that is visible under light microscopy. Thanks Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CHRISH <@t> HEALTHCARESCOUTS.COM Thu Nov 20 11:31:04 2008 From: CHRISH <@t> HEALTHCARESCOUTS.COM (Chris Handrahan) Date: Thu Nov 20 11:39:19 2008 Subject: [Histonet] Immediate Staff Need in Las Vegas Message-ID: The #1 Recruiter for Laboratory/Biotech Specialists Healthcare Scouts is a medical laboratory/biotech recruitment firm that places professionals in full-time, permanent positions throughout the United States. As the leading nationally recognized permanent placement solution for medical laboratory/biotech professionals, Healthcare Scouts has the following histology openings in Las Vegas Histotechnologists, Histo Supervisor and Histo Manager. 3-5 years of Histo experience. Must be HT (ASCP) or HTL (ASCP) certified. 5 years of experience in a leadership role. For immediate consideration please contact Chris Handrahan Managing Director of Allied Health Healthcare Scouts 800-708-0605 office 321-231-5427 cell chrish@healthcarescouts.com www.healthcarescouts.com From JWeems <@t> sjha.org Thu Nov 20 11:41:34 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Nov 20 11:41:39 2008 Subject: [Histonet] Kappa & Lambda In situ Probes In-Reply-To: References: <5D64396A0D4A5346BEBC759022AAEAA516991C@ITSSSXM01V6.one.ads.che.org> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA5169933@ITSSSXM01V6.one.ads.che.org> Oops... Sorry. Thanks for clearing that up!!! Got my vendors confused! j -----Original Message----- From: Morken, Tim [mailto:tim.morken@thermofisher.com] Sent: Thursday, November 20, 2008 12:38 PM To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kappa & Lambda In situ Probes Just a note, Leica is not affiliated with Lab Vision, but is affiliated with Vision Biosystems. Lab Vision/Thermo Fisher Scientific does not carry lambda or kappa ISH probes. Tim Morken Technical Support Manager Immunohistochemistry Anatomical Pathology Thermo Fisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, November 20, 2008 8:57 AM To: McMahon, Loralee A; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kappa & Lambda In situ Probes Check with Leica (Lab Vision). They knew about the federal changes are were prepared to handle the change. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Thursday, November 20, 2008 11:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kappa & Lambda In situ Probes We were using the Kappa and Lambda ISH probes from Dako along with their PNA kit which made them visible under light microscope. Now they have discontinued the Kappa and Lambda probe. Does anyone out there have an alternative that is visible under light microscopy. Thanks Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From kenneth.a.troutman <@t> Vanderbilt.Edu Thu Nov 20 12:05:01 2008 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Thu Nov 20 12:05:05 2008 Subject: Subject: [Histonet] Control slide storage Message-ID: <37DEF9AF72994947AF693956A59B9B6601280012@mailbe03.mc.vanderbilt.edu> I have, over the years, seen varying results on this one. Currently, we store everything at room temp except HercepTest (Her-2) slides and ER/PR slides which we now store in the fridge at 2-8 C. I had noticed that when we cut 30 or so in advance, by the time we were getting to the 15th slide or so (when they were stored at RT) they went from a 2+/3+ to a 0/1+. This phenomenon is not exclusive to breast markers, however. The best thing I can say is, keep track of your staining from day to day or week to week depending on how often you do the stain, and compare the slides to see if you are losing antigenicity. If you see that you are, try storing them in the fridge. If you are still losing antigenicity, try cutting a fresh section and run the old one and the fresh one side by side. If you are getting significantly different results, you may have to just cut a fresh section each time you run the stain (major bummer, I know, but I had a research antibody I needed to do that with...) As far as long term storage, what is long term? I am in a clinical setting right now, so "long term" to me means a month or so. If you are in research and you are talking about 7-8 months or a year or longer, you might want to think about a freezer. Hope I answered your question. Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN Message: 7 Date: Wed, 19 Nov 2008 15:31:15 -0600 From: Cindy.J.Chard-Bergstrom@aphis.usda.gov Subject: [Histonet] Control slide storage To: Message-ID: Content-Type: text/plain; charset="US-ASCII" The issue was brought up in the lab regarding the storing of IHC control slides. What experience has anyone had on the effects of long term storage and staining quality. We store the slides non preheated (with the paraffin still on them). Cindy J Chard-Bergstrom From gayle.callis <@t> bresnan.net Thu Nov 20 12:10:10 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Nov 20 12:10:11 2008 Subject: [Histonet] Stain for Cellulose References: Message-ID: <6F80872B65C14749B1036D76638CF036@DHXTS541> Tom, This is a total shot in the dark, but cellulose, being a very large long-chain, complicated polysaccharide, might stain with Grocotts Methenamine silver? You may want to try some different chromic acid oxidation times to try and enhance the cellulose, but get rid of tissue components that stain positive for GMS. In other words, overoxidize the tissue components so the cellulose is what you end up with. Collagen may still stain, but you should be able to distinguish this morphologically. Chromic acid is often done for 1 hour, so maybe a time study to pull sections at 30 minutes, 1, hr, then 1.5 hours, 2 hours. You could also try a Chromic acid Schiffs (NOT a PAS here) to see if fibers will stain dark pink compared to other tissue components. Periodic acid may not be a strong enough oxidizer to do the job. I have no clue if this would work, but it may be worth a try. Gayle M. Callis HTL(ASCP)HT,MT ----- Original Message ----- From: To: Sent: Thursday, November 20, 2008 9:36 AM Subject: [Histonet] Stain for Cellulose >I am looking for a stain that will specifically label cellulose, mixed in > with other mammalian tisssue elements. Any ideas? > > Tom Crowell > Novartis Institute for BiomedicalReseach > Cambridge, MA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pkarlisch <@t> hmc.psu.edu Thu Nov 20 12:10:24 2008 From: Pkarlisch <@t> hmc.psu.edu (Patricia Karlisch) Date: Thu Nov 20 12:10:52 2008 Subject: [Histonet] RE: Leica Slide Writer Message-ID: <492561BF.07F7.008C.0@hmc.psu.edu> Just out of curiosity. When the slide writer is working okay, do you still have to place paper labels on the slides or does the ink disappear with time??? Thank you, Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From bhewlett <@t> cogeco.ca Thu Nov 20 12:20:00 2008 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Thu Nov 20 12:20:06 2008 Subject: [Histonet] Stain for Cellulose References: Message-ID: <39AFF3D690494188B942465AA61CD700@mainbox> Tom, The alkaline Congo red method for amyloid will also stain cellulose. Examined by polarisation microscopy, only amyloid and cellulose will give the characteristic green dichroic birefringence. In addition, Congo red is fluorescent. Bryan ----- Original Message ----- From: To: Sent: Thursday, November 20, 2008 11:36 AM Subject: [Histonet] Stain for Cellulose >I am looking for a stain that will specifically label cellulose, mixed in > with other mammalian tisssue elements. Any ideas? > > Tom Crowell > Novartis Institute for BiomedicalReseach > Cambridge, MA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jcline <@t> wchsys.org Thu Nov 20 12:20:23 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Thu Nov 20 12:20:45 2008 Subject: [Histonet] Leica slide writer - problems?? In-Reply-To: <4F528252682D36478D2EA05FBECC2DE701944A7C@ALPW31.f2.enterprise> Message-ID: We have had the same problems, we discovered the slide surface on some of the snowcoat slides changed to a slick surface instead of a rough surface. We have switched to a pale gray slide that still has the rough surface and we have not had any printing wipe off. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara.Lees@sanofi-aventis.com Sent: Thursday, November 20, 2008 7:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica slide writer - problems?? Hi, Has anyone else been having problems with there Lieca slide writer. Our problems mainly involve the ink writing rubbing off before and after staining!!, we use Surgipath snowcoated slides, what does everyone else use as they have hinted it could be our slides. Any help would be fantastic. Thanks Loads Sara _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Jackie.O'Connor <@t> abbott.com Thu Nov 20 12:22:04 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Nov 20 12:22:26 2008 Subject: [Histonet] RE: Leica Slide Writer In-Reply-To: <492561BF.07F7.008C.0@hmc.psu.edu> Message-ID: We label tens of thousands of slides a year - no paper labels required. I visited a research lab in Germany - they labele 1,000 slides a day. Never had a problem with the ink coming off or disappearing - where would it go? "Patricia Karlisch" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/20/2008 12:10 PM To cc Subject [Histonet] RE: Leica Slide Writer Just out of curiosity. When the slide writer is working okay, do you still have to place paper labels on the slides or does the ink disappear with time??? Thank you, Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLunetta <@t> luhcares.org Thu Nov 20 12:27:02 2008 From: MLunetta <@t> luhcares.org (Matthew Lunetta) Date: Thu Nov 20 12:27:20 2008 Subject: [Histonet] Toe/finger nail help Message-ID: <49254986020000A800025F65@mail.luhcares.org> We are having a problem with having fixed, embedded toe/finger nails fall off of charged slides. Are there any tricks out in histoworld in avoiding this problem? Usually it is during the GMS (microwave) staining. the H&E's stay on. We figure it is the heat from the staining process. Any help? thanks, Matt Lunetta HT, Longmont United Hospital From Samuel_Perry <@t> DFCI.HARVARD.EDU Thu Nov 20 12:29:07 2008 From: Samuel_Perry <@t> DFCI.HARVARD.EDU (Perry, Samuel) Date: Thu Nov 20 12:29:11 2008 Subject: [Histonet] pap pen with vecter antifade Message-ID: Hi All, I am wondering how people do remove pap pen when they need to cover slip with an aqueous mounting media. Thanks Sam The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From gu.lang <@t> gmx.at Thu Nov 20 12:44:44 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Nov 20 12:45:10 2008 Subject: AW: [Histonet] Stain for Cellulose In-Reply-To: References: <200811201548.mAKFmoIM001492@ch1ssaenov02.novartis.com> Message-ID: <07CDF158058A4076A69D1CD5B3366C8D@dielangs.at> This link shows a german website about biological(botanical) stains. There are some, that refer to cellulose-staining. http://www.aeisner.de/index.html Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von thomas.crowell@novartis.com Gesendet: Donnerstag, 20. November 2008 17:37 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Stain for Cellulose I am looking for a stain that will specifically label cellulose, mixed in with other mammalian tisssue elements. Any ideas? Tom Crowell Novartis Institute for BiomedicalReseach Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Nov 20 12:52:35 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Nov 20 12:53:00 2008 Subject: AW: [Histonet] Kappa & Lambda In situ Probes In-Reply-To: <2CF6F6B05263EA4EBAB07781B51E5DB0028AE9C0@e2k3ms1.urmc-sh.rochester.edu> References: <2CF6F6B05263EA4EBAB07781B51E5DB0028AE9C0@e2k3ms1.urmc-sh.rochester.edu> Message-ID: <5672B6CA4130451A80C592036CC45E88@dielangs.at> The producer is Zytovision and the product is called Zytofast. The distributor is Axxora in USA. http://www.zytovision.com/index.html Zytofast Kappa, Lambda or double-stain is a CISH-Kit. They also sell biotinylated or digoxinylated probes. Until now I've no experiences with these products. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von McMahon, Loralee A Gesendet: Donnerstag, 20. November 2008 17:51 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Kappa & Lambda In situ Probes We were using the Kappa and Lambda ISH probes from Dako along with their PNA kit which made them visible under light microscope. Now they have discontinued the Kappa and Lambda probe. Does anyone out there have an alternative that is visible under light microscopy. Thanks Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Nov 20 12:57:13 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Nov 20 12:57:35 2008 Subject: AW: AW: [Histonet] Control slide storage In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1744E1B461A@EXC-MBX3.cfs.le.ac.uk> References: <46AAF0DCDC8E465F87AB0F21303EBCF8@dielangs.at> <7722595275A4DD4FA225B92CDBF174A1744E1B461A@EXC-MBX3.cfs.le.ac.uk> Message-ID: <6BD906F704EC4EFF9771104067EEBE1E@dielangs.at> I think the culprit is a "kind of oxidation" the epitopes suffer from. But it would be interesting, if the fixation is a factor of influence, or if the composition of the epitope itself makes the difference. Gudrun -----Urspr?ngliche Nachricht----- Von: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Gesendet: Donnerstag, 20. November 2008 17:34 An: 'Jackie M O'Connor'; gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Betreff: RE: AW: [Histonet] Control slide storage Is it quite as simple as that, I imagine that many epitopes might lose "stainability" if fixation is inadequate or processing poorly controlled. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: 20 November 2008 15:47 To: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Control slide storage CD31 for sure. I do recall most surface markers I used tended to be unstable. Jackie O' "Gudrun Lang" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/20/2008 09:17 AM Please respond to gu.lang@gmx.at To cc Subject AW: [Histonet] Control slide storage Hi Patsy, I am interested in the names of this certain epitopes, that loose stainability after long time storage. And I'd like to ask all the other listmembers about their experiences with this issue. Perhaps we can make a list of "dangerous" epitopes. Regards Gudrun Lang Akh Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Patsy Ruegg Gesendet: Donnerstag, 20. November 2008 15:09 An: Cindy.J.Chard-Bergstrom@aphis.usda.gov; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Control slide storage I store all cut paraffin sections for IHC control at 4dc and without heating to melt the paraffin, even with that I have seen some loss of antigenicity over long periods of time for certain antibodies. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy.J.Chard-Bergstrom@aphis.usda.gov Sent: Wednesday, November 19, 2008 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control slide storage The issue was brought up in the lab regarding the storing of IHC control slides. What experience has anyone had on the effects of long term storage and staining quality. We store the slides non preheated (with the paraffin still on them). Cindy J Chard-Bergstrom _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Thu Nov 20 12:57:47 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Nov 20 12:58:36 2008 Subject: [Histonet] RE: Leica Slide Writer In-Reply-To: References: <492561BF.07F7.008C.0@hmc.psu.edu> Message-ID: <012601c94b41$e9e49940$bdadcbc0$@com> All of this conversation about ink and slides reminds of the same issue with paper labels. It's not JUST the ink, or JUST the printer, or JUST the label (if using labels). It's ALWAYS about the combination of all the components working together. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, November 20, 2008 12:22 PM To: Patricia Karlisch Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Leica Slide Writer We label tens of thousands of slides a year - no paper labels required. I visited a research lab in Germany - they labele 1,000 slides a day. Never had a problem with the ink coming off or disappearing - where would it go? "Patricia Karlisch" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/20/2008 12:10 PM To cc Subject [Histonet] RE: Leica Slide Writer Just out of curiosity. When the slide writer is working okay, do you still have to place paper labels on the slides or does the ink disappear with time??? Thank you, Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmweber113 <@t> comcast.net Thu Nov 20 13:01:36 2008 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Thu Nov 20 13:01:44 2008 Subject: [Histonet] Positions Phoenix Az Message-ID: <112020081901.21947.4925B41000002AE8000055BB2215553894CCCECE9D0A0D0A99039D@comcast.net> I have 3 full-time positions with 1 possible part-time position available in a state of the art new in-house GI laboratory in Phoenix Az. This is for a 39 man GI group with reaching a goal of 48,000 bx a year with 16,000 IHC a year. Candidates must be ASCP certified, detailed oriented, well organized and can work as a team member. We offer $500 bounty for anyone referring a successful candidate that stays with us for at least 90 days. Interested candidates can email resume to above email or to twincrest@bex.net or call 732 814-1170 for further Thank you, Twincrest LLC From microwave <@t> ebsciences.com Thu Nov 20 13:16:01 2008 From: microwave <@t> ebsciences.com (Tracy Cloyd) Date: Thu Nov 20 13:16:21 2008 Subject: Message-ID: <8CB1889E0376F3A-5CC-8BD@WEBMAIL-MB09.sysops.aol.com> [Histonet] ThermoWave users Message-ID: <4925B771.4020602@ebsciences.com> Roxanne, Here is a protocol that our customers use and like. (None of the microwave times listed include ramp time) 1. Fix your samples 2. Room temperature fix in Preserve or other Formalin free fixative, for 30 minutes (This step is intended if you should decide to use a Formalin free fixative after the tissue has been in Formalin) 3. Microwave fix for 4 minutes at 55?C. 4. Microwave in 100% ethyl alcohol for 4 minutes at 67?C. 5. Microwave in 100%isopropyl alcohol for 4 minutes at 74?C. 6. Microwave in paraffin for 7 minutes at 84?C. (use Polar Heat sheet) We have more should you want others~ -- Regards, Tracy Tracy Cloyd Microwave Product Manager Energy Beam Sciences, Inc. Toll Free 1(800)-992-9037 ext. 341 (860)-653-0411 ext. 341 www.ebsciences.com From dhall <@t> incytepathology.com Thu Nov 20 13:16:45 2008 From: dhall <@t> incytepathology.com (Diane Cadorette-Hall) Date: Thu Nov 20 13:16:48 2008 Subject: [Histonet] Slide Printers Message-ID: <706224670091FE47997AEF88EFADE7CAA3D94A@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Hello Everyone, I am looking for a small slide printer that works off a bar code printed on the cassette. There are some small ones on the market that can sit right next to the microtome for each tech to use. Anyone have any experience with these printers? Thanks! Diane Diane Cadorette-Hall Histology Laboratory Supervisor InCyte Pathology 13103 East Mansfield Avenue Spokane, Washington 99216 509-892-2744 dhall@incytepathology.com From kdboydhisto <@t> yahoo.com Thu Nov 20 13:48:34 2008 From: kdboydhisto <@t> yahoo.com (KELLY BOYD) Date: Thu Nov 20 13:48:37 2008 Subject: [Histonet] Leica slide writer - problems?? Message-ID: <581405.20519.qm@web58601.mail.re3.yahoo.com> Hi all. I have used a lot of different slides on it too. The best ones I have found are the Starfrost clipped corner slides by Mercedes. The ink looks great and they don't get "stuck"?Great price too! Kelly --- On Thu, 11/20/08, Judith L. Williams wrote: From: Judith L. Williams Subject: Re: [Histonet] Leica slide writer - problems?? To: Sara.Lees@sanofi-aventis.com Cc: histonet@lists.utsouthwestern.edu Date: Thursday, November 20, 2008, 12:22 PM Hi all- we have a Leica and I have tried different slides. here is my take on what works and doesn't: >Starfrost Plus or non-plus (German) don't work too well- the pink frosted slides especially - the ink wipes off- the green is better (why?), the white is ok. >Unifrost, non charged, by Ever Scientific (VWR) works well >Super Up-Rite by Richard Allen, non-charged - terrible! bleeds and you can't read it >Colormark Plus by Thermo Sci. for labeling instruments - works Beautifully - this is the one I will go with in future orders! Judy in Washington On Thu, 20 Nov 2008 Sara.Lees@sanofi-aventis.com wrote: > Hi, > > Has anyone else been having problems with there Lieca slide writer. Our > problems mainly involve the ink writing rubbing off before and after > staining!!, we use Surgipath snowcoated slides, what does everyone else > use as they have hinted it could be our slides. Any help would be > fantastic. > > Thanks Loads > > Sara > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From elizabeth.heimrich <@t> bms.com Thu Nov 20 14:18:49 2008 From: elizabeth.heimrich <@t> bms.com (Elizabeth M Heimrich) Date: Thu Nov 20 14:18:55 2008 Subject: [Histonet] Human tissue for research purposes Message-ID: <4925C629.1000909@bms.com> Hello Histonetters, I have an investigator that is in need of human tissue samples. We would need IBD/UC colon and RA joint/synovium as of right now. Does anyone know where I could locate these tissues? I found three suppliers online, but I was wondering if there are more out there. Do hospitals donate tissue for research purposes? I know there are a lot of legal/ethical issues surrounding human tissue... I know this topic has been discussed before, but the histonet archives are not available to me right now (technical issue). Thanks, Beth From cbarone <@t> NEMOURS.ORG Thu Nov 20 14:20:48 2008 From: cbarone <@t> NEMOURS.ORG (Barone, Carol ) Date: Thu Nov 20 14:20:58 2008 Subject: [Histonet] FW: help Message-ID: <37E4BAC017F57141AF64FAA5AEB04CE80147191E@wlmmsx01.nemours.org> > -----Original Message----- > From: Barone, Carol > Sent: Thursday, November 20, 2008 3:17 PM > To: 'histonet-request@lists.utsouthwestern.edu' > Subject: help > Importance: High > > Histo friends...I am working on a five year strategic plan for a Research Histotechnology Core Lab with very diverse services. So I thought I might ask... > "What trend for histology services do you all see for the future of histo?" We now do routine, specials, HC, IF and IHC, In situ, TUNEL, BrdU, LMD, morphotmetrics, paraffin, plastics, and frozens....you name it, we do it. So, other than TMA....what do we see as technical trends in histology for the next five years? > > Another question, what came first the chicken or the egg? From zodiac29 <@t> comcast.net Thu Nov 20 14:21:21 2008 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Thu Nov 20 14:21:27 2008 Subject: [Histonet] immuno set-up Message-ID: <112020082021.29733.4925C6C10003F44E000074252214756402C7CD0C0E070B0196@comcast.net> Hello all, I work at a private lab with one pathologist, and one histotech (me). We do about 5,000 cases a year. We are intrested in doing our immuno's in house (right now we send them out), and wanted to know your opinion on the type of equipment that would be best for this situation. Right now we send about 5 to 10 cases a week out for immuno's. All responses are very much appreciated Jenny From rjbuesa <@t> yahoo.com Thu Nov 20 14:24:49 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Nov 20 14:24:52 2008 Subject: [Histonet] Toe/finger nail help In-Reply-To: <49254986020000A800025F65@mail.luhcares.org> Message-ID: <83088.52204.qm@web65715.mail.ac4.yahoo.com> Matthew: Under separate cover I am forwarding a material I wrote on the subject that I am sure will help you. Ren? J. --- On Thu, 11/20/08, Matthew Lunetta wrote: From: Matthew Lunetta Subject: [Histonet] Toe/finger nail help To: histonet@lists.utsouthwestern.edu Date: Thursday, November 20, 2008, 1:27 PM We are having a problem with having fixed, embedded toe/finger nails fall off of charged slides. Are there any tricks out in histoworld in avoiding this problem? Usually it is during the GMS (microwave) staining. the H&E's stay on. We figure it is the heat from the staining process. Any help? thanks, Matt Lunetta HT, Longmont United Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu Nov 20 16:02:40 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Nov 20 16:02:45 2008 Subject: AW: [Histonet] Control slide storage In-Reply-To: <6BD906F704EC4EFF9771104067EEBE1E@dielangs.at> Message-ID: There has been much discussion on this issue in the past so be sure and check the Histonet Archives. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Thursday, November 20, 2008 12:57 PM To: 'Edwards, R.E.' Cc: histonet@lists.utsouthwestern.edu Subject: AW: AW: [Histonet] Control slide storage I think the culprit is a "kind of oxidation" the epitopes suffer from. But it would be interesting, if the fixation is a factor of influence, or if the composition of the epitope itself makes the difference. Gudrun -----Urspr?ngliche Nachricht----- Von: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Gesendet: Donnerstag, 20. November 2008 17:34 An: 'Jackie M O'Connor'; gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Betreff: RE: AW: [Histonet] Control slide storage Is it quite as simple as that, I imagine that many epitopes might lose "stainability" if fixation is inadequate or processing poorly controlled. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: 20 November 2008 15:47 To: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Control slide storage CD31 for sure. I do recall most surface markers I used tended to be unstable. Jackie O' "Gudrun Lang" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/20/2008 09:17 AM Please respond to gu.lang@gmx.at To cc Subject AW: [Histonet] Control slide storage Hi Patsy, I am interested in the names of this certain epitopes, that loose stainability after long time storage. And I'd like to ask all the other listmembers about their experiences with this issue. Perhaps we can make a list of "dangerous" epitopes. Regards Gudrun Lang Akh Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Patsy Ruegg Gesendet: Donnerstag, 20. November 2008 15:09 An: Cindy.J.Chard-Bergstrom@aphis.usda.gov; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Control slide storage I store all cut paraffin sections for IHC control at 4dc and without heating to melt the paraffin, even with that I have seen some loss of antigenicity over long periods of time for certain antibodies. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy.J.Chard-Bergstrom@aphis.usda.gov Sent: Wednesday, November 19, 2008 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control slide storage The issue was brought up in the lab regarding the storing of IHC control slides. What experience has anyone had on the effects of long term storage and staining quality. We store the slides non preheated (with the paraffin still on them). Cindy J Chard-Bergstrom _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ncollins <@t> system1.net Thu Nov 20 16:37:23 2008 From: ncollins <@t> system1.net (Noelle Collins) Date: Thu Nov 20 16:36:10 2008 Subject: [Histonet] Histology Supervisor- Ohio Message-ID: I am a recruiter specializing in the laboratory industry. I have a great opportunity in Cincinnati, Ohio, for an experienced Histology Supervisor. Our client is offering competitive salary, great benefits package, and great career advancement opportunities. They are willing to help with relocation if necessary. Please let me know if you have any interest. And feel free to pass along my contact information to anyone who you feel is appropriate. Thank you for your time and consideration. Best Regards, Noelle Collins Executive Recruiter System 1 Search 864-528-5065 ncollins@system1.net From sprice2003 <@t> gmail.com Thu Nov 20 17:35:55 2008 From: sprice2003 <@t> gmail.com (Sally Price) Date: Thu Nov 20 17:35:59 2008 Subject: [Histonet] RE: Dako and Leica immunostainers Message-ID: All, After reading this thread I just had offer my comments. I'm not a big fan of systems that do the dewax and AR, primarily because it costs way too much to automate these steps. I've never used the Bond, but I hear that you've gotta put some plastic thingy - that probably costs too much - on top of each slide, you gotta use their detection reagents - which probably cost more than other companies, they charge you for empty barcoded reagent containers, all the slides in the same tray have to use the same detection reagents - which means that the continuous-feed feature has some serious limits, it can't do double-stains, and they have less than 50 IVD-approved antbodies. Can someone verify for me if all this issues are true? If so, why would someone want one of these stainers? The Dako stainer is a dinosaur and with all the newre/better ones available, they should probably take it off the market. Cheers, Sally -----Original Message----- From: Josie Britton To: Michael Bradley ; Histonet@lists.utsouthwestern.edu Sent: Tue, 18 Nov 2008 11:31 am Subject: RE: [Histonet] Dako Hi, We just got the new Bond Max IHC stainer and we love it. You just cut the slides dry them and place them on the Bond. It does retrieval, antibody staining, and counter stain. You just dehydrate , clear, and mount your coverslip. It is easy to use. It has 3 individual slide tray's of 10. You can load more slides on the empty tray's and start a new batch while the others are running. We run into the pathologist's adding more antibodies to the list an hour after we have run the first batch frequently, so this feature is great. When you add more IHC's the run time on all the slide tray's run times do increase, but it's better than having to wait another 2-3 hours to put your next set of immuno's on. Hope this helps! Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 -----Original Message----- Message: 9 Date: Wed, 19 Nov 2008 17:17:52 -0500 From: godsgalnow@aol.com Subject: Re: [Histonet] Dako To: JCBRITTON@Cheshire-Med.COM, jaustin1967@gmail.com, Histonet@lists.utsouthwestern.edu Just another FYI... The Biocare IntelliPath does the same thing, but has 4 trays of 10 and it allows you to make one of the trays?RUSH.? SO depending, on the volume?of IHC you do either of these machines would work out great. Roxanne From FidgenL <@t> shmc.org Thu Nov 20 19:23:47 2008 From: FidgenL <@t> shmc.org (Fidgen, Laura L.) Date: Thu Nov 20 19:23:57 2008 Subject: [Histonet] tissue/formalin disposal Message-ID: <6BB8BC4519AAB844B174FC739A679BBC02DA8EF8@IRMEXCH01.irm.inhs.org> Hi everyone! Can I get a survey of how higher volume labs (40-50,000 cases per year) dispose of their formalin and tissues? Are there companies out there that will take both (i.e. the tissues remain in formalin in their containers)? My safety officers are asking that we strain the tissues from the formalin, neutralize the formalin, replace the tissue to their containers then have our solid waste folks get rid of it. Once neutralized, our formalin can be disposed in the sink drains. Any information would be appreciated. Laura Fidgen, HT(ASCP)cm Histology Supervisor Sacred Heart Medical Center 509-474-4418 From tkngflght <@t> yahoo.com Thu Nov 20 19:44:42 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Nov 20 19:44:41 2008 Subject: [Histonet] Dozens of job openings...histotech to histotech In-Reply-To: References: Message-ID: <9BE04A728B4E44169FF69D49DF6657B9@FULLSTAFF.ORG> Hi Everyone-- Dozens of openings--all sorts of situations: bench, research, IHC, grossing, supervisor, magagerial...and a few other variations. We have clients in 49 states (if Hawaii comes up I get first dibs!!)--all the jobs you've seen posted here are with labs that work with us, too. So then~~what sets us apart? We start by understanding what YOU want instead of with a list of jobs to fill...and we don't push. For many positions we've had temps in place and can tell you both the good and the icky. Wouldn't that be nice to know before you decide? How about a job that REALLY fits for the new year?? Finding a new job takes a month or two (or three) of serious searching and who has that kind of time? We specialize in histology with a few additional jobs for Pathologists' Assistants, Cytologist and a few Med Tech positions for people in good labs we like to work with. The conversation is easy and confidential. We'll help with resumes, coach you for your interviews and even tell you if we think the job is NOT a good fit...but we ALWAYS respect the fact that it's your life, your career and it effects your family. I have a couple of people yet to call from recent emails and I apologize for taking so long to return your inquiry. Trust that we'll take the time to get to know you~~rushing is for frozen sections!! Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time. 281.913.7285 office 281.913.7288 fax 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org From tkngflght <@t> yahoo.com Thu Nov 20 20:06:31 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Nov 20 20:06:29 2008 Subject: [Histonet] Experienced temp for third shift needed... Message-ID: <6A8CB8A8F77F4FEEA331270381B4B85A@FULLSTAFF.ORG> Hi all-- Seeking an experienced temp who likes third shift...this is for a client I REALLY like working with alongside several of our awsome traveling histotemps already in place. (We have GREAT temps!! THANKS GUYS!!) I'll be out of office for a couple of hours tomorrow (eeek! The Twilight movie opens and a bunch of 'tween girls on a 13th birthday wish---wish ME luck!!) but will be back in the afternoon and will return all inquiries before the end of the weekend-- Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing Healthcare Professionals - One GREAT fit at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org From rachae19 <@t> mindspring.com Thu Nov 20 21:11:23 2008 From: rachae19 <@t> mindspring.com (Libby White) Date: Thu Nov 20 21:11:36 2008 Subject: [Histonet] help with Zeiss Apotome Message-ID: <020101c94b86$d5be9e30$813bda90$@com> I have inherited a new Zeiss Axiovert system with an Apotome. Our sales rep has helped me to learn the system but he says that Zeiss has no user manual for this microscope and attachments. Does anyone have a protocol for using these instruments, possibly a core facility with multiple users. Thanks for any help you can send my way, Libby White Centers for Disease Control Atlanta, Ga From gvdobbin <@t> ihis.org Thu Nov 20 22:23:32 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Nov 20 22:23:57 2008 Subject: [Histonet] RE: Dako and Leica immunostainers Message-ID: Sally, I think you answered your own question! You wrote: "If so, why would someone want one of these stainers?" And yet, they sell so many! I have found the Bond to be an excellent instrument and in my opinion it is unrivalled in the market place. I have just recently spent 10 months doing extensive evaluations of Bond, Dako and Ventana. I brought each instrument in-house to try. When all was said and done the Bond was the best fit for my lab. Each lab needs to evaluate what is the best fit for their particular situation (ie number of staff, experience level of staff, volume of tests, workflow, etc.). But with regard to the cost for online dewax and retreival: what is your time worth? Doing these steps off-line means more tech time, more opportunity for mistakes and opens the possibility of peripheral instrumentation failing and holding everything up down the line. As for the cost per slide: I spent many agonizing hours going over pricing info for all 3 instruments (trying to "uncode" the sales speak) and in the end (by my calculations) the difference between 2 of the 3 was less than a dollar per test and the 3rd was considerably higher. I won't say which one is which, but I will say the Bond was not the higher of the 3. Hope this has helped. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "And in the end it's not the years in your life that count. It's the life in your years." - Abraham Lincoln >>> "Sally Price" 11/20/2008 7:35 PM >>> All, After reading this thread I just had offer my comments. I'm not a big fan of systems that do the dewax and AR, primarily because it costs way too much to automate these steps. I've never used the Bond, but I hear that you've gotta put some plastic thingy - that probably costs too much - on top of each slide, you gotta use their detection reagents - which probably cost more than other companies, they charge you for empty barcoded reagent containers, all the slides in the same tray have to use the same detection reagents - which means that the continuous-feed feature has some serious limits, it can't do double-stains, and they have less than 50 IVD-approved antbodies. Can someone verify for me if all this issues are true? If so, why would someone want one of these stainers? The Dako stainer is a dinosaur and with all the newre/better ones available, they should probably take it off the market. Cheers, Sally -----Original Message----- From: Josie Britton To: Michael Bradley ; Histonet@lists.utsouthwestern.edu Sent: Tue, 18 Nov 2008 11:31 am Subject: RE: [Histonet] Dako Hi, We just got the new Bond Max IHC stainer and we love it. You just cut the slides dry them and place them on the Bond. It does retrieval, antibody staining, and counter stain. You just dehydrate , clear, and mount your coverslip. It is easy to use. It has 3 individual slide tray's of 10. You can load more slides on the empty tray's and start a new batch while the others are running. We run into the pathologist's adding more antibodies to the list an hour after we have run the first batch frequently, so this feature is great. When you add more IHC's the run time on all the slide tray's run times do increase, but it's better than having to wait another 2-3 hours to put your next set of immuno's on. Hope this helps! Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 -----Original Message----- Message: 9 Date: Wed, 19 Nov 2008 17:17:52 -0500 From: godsgalnow@aol.com Subject: Re: [Histonet] Dako To: JCBRITTON@Cheshire-Med.COM, jaustin1967@gmail.com, Histonet@lists.utsouthwestern.edu Just another FYI... The Biocare IntelliPath does the same thing, but has 4 trays of 10 and it allows you to make one of the trays?RUSH.? SO depending, on the volume?of IHC you do either of these machines would work out great. Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From louise.renton <@t> gmail.com Fri Nov 21 01:47:53 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Nov 21 01:47:57 2008 Subject: [Histonet] Osteoid identifiction Message-ID: Hi al, I have received a sample of ?bone where the clinician wants to identfy osteoid. According to my knowledge (now perhaps a little daed) the traditional processing method is plastic embedding of some sort. Is this still the case or are there methods to use standars paraffin processing? Best regards-- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From pieronelva01 <@t> bigpond.com Fri Nov 21 02:11:47 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Fri Nov 21 02:11:55 2008 Subject: AW: [Histonet] Control slide storage References: <46AAF0DCDC8E465F87AB0F21303EBCF8@dielangs.at><7722595275A4DD4FA225B92CDBF174A1744E1B461A@EXC-MBX3.cfs.le.ac.uk> <6BD906F704EC4EFF9771104067EEBE1E@dielangs.at> Message-ID: <0F08B4D3EA174320BEE8B167E9AA696E@pentium4> I'd love to know why and how, because I see it regularly. A definite fading of staining intensity on identical, but older, sections. I notice it with CD10, among others. Piero Nelva From ree3 <@t> leicester.ac.uk Fri Nov 21 06:15:21 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Nov 21 06:16:02 2008 Subject: AW: [Histonet] Control slide storage In-Reply-To: <6BD906F704EC4EFF9771104067EEBE1E@dielangs.at> References: <46AAF0DCDC8E465F87AB0F21303EBCF8@dielangs.at> <7722595275A4DD4FA225B92CDBF174A1744E1B461A@EXC-MBX3.cfs.le.ac.uk> <6BD906F704EC4EFF9771104067EEBE1E@dielangs.at> Message-ID: <7722595275A4DD4FA225B92CDBF174A1744E1B4621@EXC-MBX3.cfs.le.ac.uk> I feel that as far as surgical human samples that were originally taken for a quick diagnoses( which means minimal NBF fixation) with a just H@E, the first 50-100u into the block is fine, after that who knows how well it is fixed/processed and this is where the repeat sections are taken for subsequent immunostaining, which could lead to dodgy results; just a thought!! -----Original Message----- From: Gudrun Lang [mailto:gu.lang@gmx.at] Sent: 20 November 2008 18:57 To: 'Edwards, R.E.' Cc: histonet@lists.utsouthwestern.edu Subject: AW: AW: [Histonet] Control slide storage I think the culprit is a "kind of oxidation" the epitopes suffer from. But it would be interesting, if the fixation is a factor of influence, or if the composition of the epitope itself makes the difference. Gudrun -----Urspr?ngliche Nachricht----- Von: Edwards, R.E. [mailto:ree3@leicester.ac.uk] Gesendet: Donnerstag, 20. November 2008 17:34 An: 'Jackie M O'Connor'; gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Betreff: RE: AW: [Histonet] Control slide storage Is it quite as simple as that, I imagine that many epitopes might lose "stainability" if fixation is inadequate or processing poorly controlled. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: 20 November 2008 15:47 To: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Control slide storage CD31 for sure. I do recall most surface markers I used tended to be unstable. Jackie O' "Gudrun Lang" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/20/2008 09:17 AM Please respond to gu.lang@gmx.at To cc Subject AW: [Histonet] Control slide storage Hi Patsy, I am interested in the names of this certain epitopes, that loose stainability after long time storage. And I'd like to ask all the other listmembers about their experiences with this issue. Perhaps we can make a list of "dangerous" epitopes. Regards Gudrun Lang Akh Linz, Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Patsy Ruegg Gesendet: Donnerstag, 20. November 2008 15:09 An: Cindy.J.Chard-Bergstrom@aphis.usda.gov; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Control slide storage I store all cut paraffin sections for IHC control at 4dc and without heating to melt the paraffin, even with that I have seen some loss of antigenicity over long periods of time for certain antibodies. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy.J.Chard-Bergstrom@aphis.usda.gov Sent: Wednesday, November 19, 2008 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control slide storage The issue was brought up in the lab regarding the storing of IHC control slides. What experience has anyone had on the effects of long term storage and staining quality. We store the slides non preheated (with the paraffin still on them). Cindy J Chard-Bergstrom _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mikael.niku <@t> helsinki.fi Fri Nov 21 06:41:47 2008 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Fri Nov 21 06:41:53 2008 Subject: [Histonet] Anti-fluorophore antibodies (for MOM work) In-Reply-To: <0F08B4D3EA174320BEE8B167E9AA696E@pentium4> References: <46AAF0DCDC8E465F87AB0F21303EBCF8@dielangs.at><7722595275A4DD4FA225B92CDBF174A1744E1B461A@EXC-MBX3.cfs.le.ac.uk><6BD906F704EC4EFF9771104067EEBE1E@dielangs.at> <0F08B4D3EA174320BEE8B167E9AA696E@pentium4> Message-ID: <00b301c94bd6$848282c0$97a5d680@mmkem12636> Dear Histonetters, I'd like to ask for recommendations for anti-fluorophore antibodies. I'm planning to use a fluorophore-labelled primary antibody for mouse-on-mouse IHC. This is then detected using a labelled anti-rabbitIg (or whatever host it is) ab. Apparently Dako has discontinued the unlabelled rabbit anti-FITC antibody that I understand used to be popular in these applications, and I was told the remaining HRP version doesn't work well for subsequent detection with an antiIg ab. Has anyone tried the rabbit anti-Alexa488 antibody by Molecular Probes / Invitrogen? Or are there any other good anti-FITC abs available? With best regards, Mikael Niku ----------------------------------------------------- Mikael Niku, PhD, university lecturer University of Helsinki, Division of Nutrition URL: mikael.nikunnakki.info - What do I think of western civilization? I think it would be a good idea! Gandhi ----------------------------------------------------- From cristiana.soldani <@t> humanitas.it Fri Nov 21 06:53:08 2008 From: cristiana.soldani <@t> humanitas.it (SOLDANI Cristiana ICH) Date: Fri Nov 21 06:53:19 2008 Subject: R: [Histonet] Anti-fluorophore antibodies (for MOM work) In-Reply-To: <00b301c94bd6$848282c0$97a5d680@mmkem12636> References: <46AAF0DCDC8E465F87AB0F21303EBCF8@dielangs.at><7722595275A4DD4FA225B92CDBF174A1744E1B461A@EXC-MBX3.cfs.le.ac.uk><6BD906F704EC4EFF9771104067EEBE1E@dielangs.at><0F08B4D3EA174320BEE8B167E9AA696E@pentium4> <00b301c94bd6$848282c0$97a5d680@mmkem12636> Message-ID: <8D4E4E312BEF164997299EE5B0E12B7C025580D9@TEHWEX11.techosp.it> I used converter POD from roche and it works good -----Messaggio originale----- Da: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Per conto di Mikael Niku Inviato: venerd? 21 novembre 2008 13.42 A: histonet@lists.utsouthwestern.edu Oggetto: [Histonet] Anti-fluorophore antibodies (for MOM work) Dear Histonetters, I'd like to ask for recommendations for anti-fluorophore antibodies. I'm planning to use a fluorophore-labelled primary antibody for mouse-on-mouse IHC. This is then detected using a labelled anti-rabbitIg (or whatever host it is) ab. Apparently Dako has discontinued the unlabelled rabbit anti-FITC antibody that I understand used to be popular in these applications, and I was told the remaining HRP version doesn't work well for subsequent detection with an antiIg ab. Has anyone tried the rabbit anti-Alexa488 antibody by Molecular Probes / Invitrogen? Or are there any other good anti-FITC abs available? With best regards, Mikael Niku ----------------------------------------------------- Mikael Niku, PhD, university lecturer University of Helsinki, Division of Nutrition URL: mikael.nikunnakki.info - What do I think of western civilization? I think it would be a good idea! Gandhi ----------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Fri Nov 21 08:07:18 2008 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Nov 21 08:09:33 2008 Subject: [Histonet] RE: tissue/formalin disposal In-Reply-To: <6BB8BC4519AAB844B174FC739A679BBC02DA8EF8@IRMEXCH01.irm.inhs.org> References: <6BB8BC4519AAB844B174FC739A679BBC02DA8EF8@IRMEXCH01.irm.inhs.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D2F0F96E3@LRGHEXVS1.practice.lrgh.org> That is exactly how we dispose of our tissue. We decant off the formalin, de-formalize and test it and if it passes rinse down the drain. By the way I had to get NH state approval to do this. The tissue is bagged, boxed and then hauled off for disposal. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fidgen, Laura L. [FidgenL@shmc.org] Sent: Thursday, November 20, 2008 8:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue/formalin disposal Hi everyone! Can I get a survey of how higher volume labs (40-50,000 cases per year) dispose of their formalin and tissues? Are there companies out there that will take both (i.e. the tissues remain in formalin in their containers)? My safety officers are asking that we strain the tissues from the formalin, neutralize the formalin, replace the tissue to their containers then have our solid waste folks get rid of it. Once neutralized, our formalin can be disposed in the sink drains. Any information would be appreciated. Laura Fidgen, HT(ASCP)cm Histology Supervisor Sacred Heart Medical Center 509-474-4418 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Mark.Frei <@t> sial.com Fri Nov 21 08:56:33 2008 From: Mark.Frei <@t> sial.com (Mark Frei) Date: Fri Nov 21 08:57:08 2008 Subject: [Histonet] Re:Control slide storage In-Reply-To: <200811210147.mAL1l536082849@stlspfw2.sial.com> Message-ID: Just as a frame of reference, alkaline phosphatase is a very labile enzyme that can loose activity quickly in storage. For alk phos histochemistry controls, we store fixed peripheral blood smears individually wrapped in parafilm at -70 C for up to a year with minimal loss of activity. Mark Frei MT(ASCP) Sigma-Aldrich Hematology & Histology 3050 Spruce Street / Saint Louis, MO, 63103 / USA P: (800) 771-5765, x4164 / Direct: (314) 286-8080 sigma-aldrich.com This message and any files transmitted with it are the property of Sigma-Aldrich Corporation, are confidential, and are intended solely for the use of the person or entity to whom this e-mail is addressed. If you are not one of the named recipient(s) or otherwise have reason to believe that you have received this message in error, please contact the sender and delete this message immediately from your computer. Any other use, retention, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. From christina.thurby <@t> bms.com Fri Nov 21 09:30:26 2008 From: christina.thurby <@t> bms.com (Christina Thurby) Date: Fri Nov 21 09:30:39 2008 Subject: [Histonet] Does anyone have the link for the free LCM webinar that was on histonet this week? In-Reply-To: <0KAN00G1NU7RV1@dakia.bms.com> References: <0KAN00G1NU7RV1@dakia.bms.com> Message-ID: <4926D412.1040001@bms.com> Does anyone have the link for the free LCM webinar that was on histonet this week? I'd like to register but have deleted the post from my e-mail and I can not find it in the archives yet. Thanks, Christina Thurby Bristol Myers Squibb 812-429-8097 From eca9 <@t> georgetown.edu Fri Nov 21 10:11:07 2008 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Fri Nov 21 10:11:15 2008 Subject: [Histonet] bluing hematoxylin and alkaline water???? Message-ID: <4926DD9B.3000108@georgetown.edu> Good morning out in histoland, Thank god it is Friday. Have just a small question that I am sure you all have an answer for. It is about bluing slides after hematoxylin. I read somewhere that alkaline water can blue hematoxylin and I am wondering if this is part of our problem. We use an automated stainer that we run both during the day and during the night. Normally we blue the slides in 1% ammonium after the runs for 1min. The run that was done during the day ended up less blue (more purple). The ones that run during the night continue being washed in water until we come in in the morning. They ended up more blue. I tested the pH of the water we are using. It is 7.96. Would this be enough to blue the slides if they are washed in it every hour from 9pm to 8am? Any other reason this might be happening? Should we not blue the slides that are run during the night? Why do we blue hematoxylin anyways? Thank you for all your answers Eva Permaul (still learning) From sbresson <@t> wtbyhosp.org Fri Nov 21 10:42:59 2008 From: sbresson <@t> wtbyhosp.org (Bresson, Sarah) Date: Fri Nov 21 10:43:03 2008 Subject: [Histonet] Blueing Hematoxylin Message-ID: <0A57D6AEAE4CBA4A984D27257160A72D010A031C@win03exchange01.wtbyhosp.org> My lab does not blue the hematoxylin. Some say that it makes the nuclei stand out more, but you don't have to. You can sometimes over blue as well. From rjbuesa <@t> yahoo.com Fri Nov 21 10:55:51 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 21 10:55:55 2008 Subject: [Histonet] bluing hematoxylin and alkaline water???? In-Reply-To: <4926DD9B.3000108@georgetown.edu> Message-ID: <156204.87316.qm@web65704.mail.ac4.yahoo.com> Eva: Hematoxylin is a pH indicator, it turns reddish when you differentiate it in the acid alcohol, and returns to "violet-blue" when you either "blue" it with the ammonia water or place it in tap water after the alcohol-acid solution. The differentiation you know is to make the nuclear details more visible and the bluing is essentially done to stop the acid effect of the clearing, that could end decolorizing the section. It is a good practice to "blue" with the alkaline (ammonia) solution or just plain tap water if it is "hard" enough (containing salts), but it is not good practice to let the slides in running water for long periods of time because, being the "universal solvent", running water will end making the stain fainter, as you have noticed in?the difference between the two types of batches you mention. It would preferable to leave the last batch in your clearing (pre-coverslipping) liquid (usually xylene), this will not affect the staining intensity at all. Ren? J. --- On Fri, 11/21/08, Eva Permaul wrote: From: Eva Permaul Subject: [Histonet] bluing hematoxylin and alkaline water???? To: histonet@lists.utsouthwestern.edu Date: Friday, November 21, 2008, 11:11 AM Good morning out in histoland, Thank god it is Friday. Have just a small question that I am sure you all have an answer for. It is about bluing slides after hematoxylin. I read somewhere that alkaline water can blue hematoxylin and I am wondering if this is part of our problem. We use an automated stainer that we run both during the day and during the night. Normally we blue the slides in 1% ammonium after the runs for 1min. The run that was done during the day ended up less blue (more purple). The ones that run during the night continue being washed in water until we come in in the morning. They ended up more blue. I tested the pH of the water we are using. It is 7.96. Would this be enough to blue the slides if they are washed in it every hour from 9pm to 8am? Any other reason this might be happening? Should we not blue the slides that are run during the night? Why do we blue hematoxylin anyways? Thank you for all your answers Eva Permaul (still learning) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Nov 21 11:01:40 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Nov 21 11:02:06 2008 Subject: AW: [Histonet] bluing hematoxylin and alkaline water???? In-Reply-To: <4926DD9B.3000108@georgetown.edu> References: <4926DD9B.3000108@georgetown.edu> Message-ID: <28B37DF9A3984EC7B84664DD2115C375@dielangs.at> Is this a Haematoxylin-Eosin-stain? If so, I think you wash out the Eosin during the long water-wash. That makes the difference. Haemalaun binds to negative charged tissueparts best in acid solution (pH3-4). With this pH the colour is redish. Blueing means to elevate the pH about 7-8. Now the bound dye-molecule is remodeld. The colour turns blue and is more stable. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Eva Permaul Gesendet: Freitag, 21. November 2008 17:11 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] bluing hematoxylin and alkaline water???? Good morning out in histoland, Thank god it is Friday. Have just a small question that I am sure you all have an answer for. It is about bluing slides after hematoxylin. I read somewhere that alkaline water can blue hematoxylin and I am wondering if this is part of our problem. We use an automated stainer that we run both during the day and during the night. Normally we blue the slides in 1% ammonium after the runs for 1min. The run that was done during the day ended up less blue (more purple). The ones that run during the night continue being washed in water until we come in in the morning. They ended up more blue. I tested the pH of the water we are using. It is 7.96. Would this be enough to blue the slides if they are washed in it every hour from 9pm to 8am? Any other reason this might be happening? Should we not blue the slides that are run during the night? Why do we blue hematoxylin anyways? Thank you for all your answers Eva Permaul (still learning) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Shakun.Aswani <@t> acologix.com Fri Nov 21 11:03:08 2008 From: Shakun.Aswani <@t> acologix.com (Shakun Aswani) Date: Fri Nov 21 11:03:16 2008 Subject: [Histonet] Does anyone have the link for the free LCM webinar that was on histonet this week? In-Reply-To: <4926D412.1040001@bms.com> References: <0KAN00G1NU7RV1@dakia.bms.com> <4926D412.1040001@bms.com> Message-ID: <777AB0DE519C8E46A6220E2287C5BAD3019BAC60@EXCHANGE.acologix.com> Christina, Enclosed is the LCM webinar Shakun Announcing MDS Analytical Technologies next in the series of Laser Capture Microdissection and Microgenomics Webinars. Our next LCM webinar will be held on Monday, November 24, 2008 at 10AM PST or 1PM EST. The speaker will be Charmain Pietersen, PhD., Laboratory for Structural and Molecular Neuroscience, McLean Hospital. Her presentation is titled: "Gene expression analysis in homogenous single cell populations in the superior temporal gyrus in postmortem brains from subjects with schizophrenia". This webinar is free to all registrants. For further information and/or to register for this webinar, please connect to the following website: http://www.moleculardevices.com/pages/lcm_webinar_11.2008.html Shirley Chu Application Scientist, Arcturus LCM Products Molecular Devices (now a part of MDS Analytical Technologies) 408-747-3765 | www.moleculardevices.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christina Thurby Sent: Friday, November 21, 2008 7:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Does anyone have the link for the free LCM webinar that was on histonet this week? Does anyone have the link for the free LCM webinar that was on histonet this week? I'd like to register but have deleted the post from my e-mail and I can not find it in the archives yet. Thanks, Christina Thurby Bristol Myers Squibb 812-429-8097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Shakun.Aswani <@t> acologix.com Fri Nov 21 11:22:12 2008 From: Shakun.Aswani <@t> acologix.com (Shakun Aswani) Date: Fri Nov 21 11:22:17 2008 Subject: [Histonet] Osteoid identifiction In-Reply-To: References: Message-ID: <777AB0DE519C8E46A6220E2287C5BAD3019BAC65@EXCHANGE.acologix.com> Hi Louise, Yes, you are right best way to quantify the osteoid is plastic embedding (MMA) but recently I saw a paper in "Stain Technology" the title is "Silver Staining of bone prior to decalcification for quantitative determination of osteoid in sections" by E.J. Tripp and E.H. MaoKay, dept of pathology, Frenchay hospital, Bristol, England. Try that paper If you don't have excess to the paper please do let me know I will send you my copy. Good Luck! Shakun -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Thursday, November 20, 2008 11:48 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Osteoid identifiction Hi al, I have received a sample of ?bone where the clinician wants to identfy osteoid. According to my knowledge (now perhaps a little daed) the traditional processing method is plastic embedding of some sort. Is this still the case or are there methods to use standars paraffin processing? Best regards-- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From teresa.p.wylie <@t> gsk.com Fri Nov 21 11:27:29 2008 From: teresa.p.wylie <@t> gsk.com (teresa.p.wylie@gsk.com) Date: Fri Nov 21 11:27:48 2008 Subject: [Histonet] RE: Leica Slide Writer In-Reply-To: <200811210119.mAL1JcJ2028113@stvsfimr002.ggr.co.uk> Message-ID: Is the flash on your printer working? The ink will smear or wash off during staining if the flash does "cure" the ink. Teresa Wylie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara.Lees@sanofi-aventis.com Sent: Thursday, November 20, 2008 7:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica slide writer - problems?? Hi, Has anyone else been having problems with there Lieca slide writer. Our problems mainly involve the ink writing rubbing off before and after staining!!, we use Surgipath snowcoated slides, what does everyone else use as they have hinted it could be our slides. Any help would be fantastic. Thanks Loads Sara From Rcartun <@t> harthosp.org Fri Nov 21 12:02:54 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Nov 21 12:03:08 2008 Subject: [Histonet] TFE3 testing Message-ID: <4926B17E0200007700007045@gwmail4.harthosp.org> Does anyone do TFE3 gene testing on renal cell CA in children? If so, do you accept cases for consultation? Can formalin-fixed, paraffin-embedded tissue be used? Billing mechanics? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From Teri.Hallada <@t> midmichigan.org Fri Nov 21 12:17:03 2008 From: Teri.Hallada <@t> midmichigan.org (Teri.Hallada@midmichigan.org) Date: Fri Nov 21 12:17:17 2008 Subject: [Histonet] Floaters Message-ID: <8839B08E3ED7364E8CBBD53882C984D50994CD57@MAILSRV01.midmichigan.net> Would anyone care to share their policy on floaters? Also, does anyone know if there is a CAP policy in floaters? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. From kenneth.a.troutman <@t> Vanderbilt.Edu Fri Nov 21 12:19:32 2008 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Fri Nov 21 12:19:49 2008 Subject: [Histonet] Human tissue for research purposes Message-ID: <37DEF9AF72994947AF693956A59B9B6601280015@mailbe03.mc.vanderbilt.edu> Human tissue for research purposes can be rather complicated. Keep in mind, your institution has requirements, the FDA has requirements and the patient has requirements. If you get the tissue from another institution...you guessed it, more requirements. I have worked with Institutional Review Boards (IRBs) with this and with some things you need the patient's permission. Some hospitals (usually teaching hospitals and large research institutions) have this covered when a patient is admitted. When the patient signs the stack of paperwork to have their surgery, there will be a small sentence that releases that tissue to be used for research purposes. The first thing I would check, though, is what the requirements for the research study are. Sometimes that can decide where your tissue comes from. Second, check with your facility administrator and the chief investigator (I don't know if you are in a hospital or a private lab--it will be different for each) about the legal issues that may surround you obtaining tissue from a source other than your own lab. The paperwork for such an endeavor can be mind-numbing. Another option would be to talk to the investigator to see if they have any colleagues that would be willing to donate tissue. (Sometimes you can get it already processed!) Getting it that way tends to be easier, especially if good documentation on the tissue already exists. If none of this works, call a major university near you and see if they have a "research tissue bank" or other such department. These guys and gals do nothing but collect various tissues and tumors for the myriad researchers at thier own institutions and may be willing to part with it. (We have something like that here at Vanderbilt, but I don't know if they sell it to outside institutions.) Sorry this is long, but I hope it helps some. Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN Message: 17 Date: Thu, 20 Nov 2008 15:18:49 -0500 From: Elizabeth M Heimrich Subject: [Histonet] Human tissue for research purposes To: histonet@lists.utsouthwestern.edu Message-ID: <4925C629.1000909@bms.com> Content-Type: text/plain; charset="iso-8859-1" Hello Histonetters, I have an investigator that is in need of human tissue samples. We would need IBD/UC colon and RA joint/synovium as of right now. Does anyone know where I could locate these tissues? I found three suppliers online, but I was wondering if there are more out there. Do hospitals donate tissue for research purposes? I know there are a lot of legal/ethical issues surrounding human tissue... I know this topic has been discussed before, but the histonet archives are not available to me right now (technical issue). Thanks, Beth From kenneth.a.troutman <@t> Vanderbilt.Edu Fri Nov 21 12:22:04 2008 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Fri Nov 21 12:22:23 2008 Subject: [Histonet] help Message-ID: <37DEF9AF72994947AF693956A59B9B6601280016@mailbe03.mc.vanderbilt.edu> The chicken came first--but she did not have a belly button... Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN -----Original Message----- > From: Barone, Carol > Sent: Thursday, November 20, 2008 3:17 PM > To: 'histonet-request@lists.utsouthwestern.edu' > Subject: help > Importance: High > > Histo friends...I am working on a five year strategic plan for a Research Histotechnology Core Lab with very diverse services. So I thought I might ask... > "What trend for histology services do you all see for the future of histo?" We now do routine, specials, HC, IF and IHC, In situ, TUNEL, BrdU, LMD, morphotmetrics, paraffin, plastics, and frozens....you name it, we do it. So, other than TMA....what do we see as technical trends in histology for the next five years? > > Another question, what came first the chicken or the egg? From ccrowder <@t> vetmed.lsu.edu Fri Nov 21 13:20:00 2008 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Fri Nov 21 13:22:40 2008 Subject: [Histonet] Sakura tape problem Message-ID: Hi - I have just been given a box (100 slides) cover slipped with Sakura tape. All the tapes have been loosened from the slides with the tissue attacked to it. The tape has also curled (arched). What is the best method for reattaching the tapes to the slides so they can be viewed again. Or can it? The pathologists are really upset over these slides as they are for continuing education and cannot be replaced. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From Jackie.O'Connor <@t> abbott.com Fri Nov 21 13:31:46 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Nov 21 13:32:06 2008 Subject: [Histonet] Sakura tape problem In-Reply-To: Message-ID: I would remove the tape with acetone and clear in xylene, then recoverslip with conventional coverslips. I've seen problems with refractivity with the tape, anyway. Happy Turkey Day. Jackie O' "Cheryl Crowder" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/21/2008 01:20 PM To "Histonet" cc Subject [Histonet] Sakura tape problem Hi - I have just been given a box (100 slides) cover slipped with Sakura tape. All the tapes have been loosened from the slides with the tissue attacked to it. The tape has also curled (arched). What is the best method for reattaching the tapes to the slides so they can be viewed again. Or can it? The pathologists are really upset over these slides as they are for continuing education and cannot be replaced. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christiegowan <@t> msn.com Fri Nov 21 13:35:49 2008 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Fri Nov 21 13:35:54 2008 Subject: [Histonet] Sakura tape problem In-Reply-To: Message-ID: ______________________________________________________________ Cherly, I had this same problem a while back with an outside slide that had been sent to us for consultation. I even called the company to get any input from them. No luck. My final solution was to trim the tape down to just the area where the section was located. I sandwiched the tape between the glass slide and a glass coverslip using mounting media only. I used a paper clamp to secure it until it dried. Do not use any xylene. This will cause the tissue to start floating off the tape and scatter around. It can be pretty messy so don't use too much mountant. Good luck and let us know if you get any better solutions. Christie From JWeems <@t> sjha.org Fri Nov 21 13:36:17 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Nov 21 13:36:22 2008 Subject: [Histonet] Sakura tape problem In-Reply-To: References: Message-ID: <5D64396A0D4A5346BEBC759022AAEAA5169B32@ITSSSXM01V6.one.ads.che.org> How do you do this with the tissue attached to the tape? Just curious... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, November 21, 2008 2:32 PM To: Cheryl Crowder Cc: histonet-bounces@lists.utsouthwestern.edu; Histonet Subject: Re: [Histonet] Sakura tape problem I would remove the tape with acetone and clear in xylene, then recoverslip with conventional coverslips. I've seen problems with refractivity with the tape, anyway. Happy Turkey Day. Jackie O' "Cheryl Crowder" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/21/2008 01:20 PM To "Histonet" cc Subject [Histonet] Sakura tape problem Hi - I have just been given a box (100 slides) cover slipped with Sakura tape. All the tapes have been loosened from the slides with the tissue attacked to it. The tape has also curled (arched). What is the best method for reattaching the tapes to the slides so they can be viewed again. Or can it? The pathologists are really upset over these slides as they are for continuing education and cannot be replaced. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Jackie.O'Connor <@t> abbott.com Fri Nov 21 13:40:11 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Nov 21 13:40:34 2008 Subject: [Histonet] Sakura tape problem In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA5169B32@ITSSSXM01V6.one.ads.che.org> Message-ID: Ahhhhh - sorry, didn't read that well. Nevermind. "Weems, Joyce" 11/21/2008 01:36 PM To "Jackie M O'Connor" , "Cheryl Crowder" cc , "Histonet" Subject RE: [Histonet] Sakura tape problem How do you do this with the tissue attached to the tape? Just curious... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, November 21, 2008 2:32 PM To: Cheryl Crowder Cc: histonet-bounces@lists.utsouthwestern.edu; Histonet Subject: Re: [Histonet] Sakura tape problem I would remove the tape with acetone and clear in xylene, then recoverslip with conventional coverslips. I've seen problems with refractivity with the tape, anyway. Happy Turkey Day. Jackie O' "Cheryl Crowder" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/21/2008 01:20 PM To "Histonet" cc Subject [Histonet] Sakura tape problem Hi - I have just been given a box (100 slides) cover slipped with Sakura tape. All the tapes have been loosened from the slides with the tissue attacked to it. The tape has also curled (arched). What is the best method for reattaching the tapes to the slides so they can be viewed again. Or can it? The pathologists are really upset over these slides as they are for continuing education and cannot be replaced. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From RSRICHMOND <@t> aol.com Fri Nov 21 14:22:09 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Nov 21 14:22:16 2008 Subject: [Histonet] Re: bluing hematoxylin and alkaline water???? Message-ID: Hard water (water containing dissolved calcium carbonate and/or sulfate is alkaline enough to use as a bluing agent by itself. New York City, Hot Springs Arkansas, and San Antonio Texas - in my personal experience - have tap water sufficiently alkaline that you can blue hematoxylin in it in a reasonable length of time. The original Scott's solution was in fact devised as a substitute for alkaline tap water. Scott SG (Oxford). On successive double staining for histological purposes?preliminary note. Journal of Pathology and Bacteriology 1911-1912: 16,390-8. Scott notes that "As tap water varies in constitution from place to place, and even the alkaline tap water of Oxford from the o?lite Cotswolds requires ten minutes with occasional changes for safe removal of acid, the artificial substitute mentioned above has been introduced." Robert Wyllie in 1970 told me that this bluing solution was widely used in Australia, apparently introduced by Oxonian histologists nostalgic for the tap water of their homeland. Wyllie was an Australian histochemist who worked in the pathology department at Johns Hopkins for a number of years. He greatly simplified Scott's original formula, and referred to this preparation as Scott's solution. Gary Gill popularized Scott's solution along with his well-known hematoxylins. Bob Wyllie (of blest memory) was an absolute genius in the histochemistry lab, but he was a very modest man. He published very little, and many of his ingenious techniques are totally lost (I have just a few of them). I spent a year as a research fellow in his laboratory in 1970. Bob Richmond Samurai Pathologist Knoxville TN From katherine-walters <@t> uiowa.edu Fri Nov 21 14:50:34 2008 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Fri Nov 21 14:50:38 2008 Subject: [Histonet] Re: bluing hematoxylin and alkaline water???? In-Reply-To: References: Message-ID: This is why I love the histonet!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Friday, November 21, 2008 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: bluing hematoxylin and alkaline water???? Hard water (water containing dissolved calcium carbonate and/or sulfate is alkaline enough to use as a bluing agent by itself. New York City, Hot Springs Arkansas, and San Antonio Texas - in my personal experience - have tap water sufficiently alkaline that you can blue hematoxylin in it in a reasonable length of time. The original Scott's solution was in fact devised as a substitute for alkaline tap water. Scott SG (Oxford). On successive double staining for histological purposes-preliminary note. Journal of Pathology and Bacteriology 1911-1912: 16,390-8. Scott notes that "As tap water varies in constitution from place to place, and even the alkaline tap water of Oxford from the o?lite Cotswolds requires ten minutes with occasional changes for safe removal of acid, the artificial substitute mentioned above has been introduced." Robert Wyllie in 1970 told me that this bluing solution was widely used in Australia, apparently introduced by Oxonian histologists nostalgic for the tap water of their homeland. Wyllie was an Australian histochemist who worked in the pathology department at Johns Hopkins for a number of years. He greatly simplified Scott's original formula, and referred to this preparation as Scott's solution. Gary Gill popularized Scott's solution along with his well-known hematoxylins. Bob Wyllie (of blest memory) was an absolute genius in the histochemistry lab, but he was a very modest man. He published very little, and many of his ingenious techniques are totally lost (I have just a few of them). I spent a year as a research fellow in his laboratory in 1970. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Fri Nov 21 15:04:34 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Nov 21 15:04:29 2008 Subject: [Histonet] Advanced Histo training Message-ID: Is there anyone out there that can answer this gentleman's questions. He is interested in getting advanced training in histotechnology over a 4 month period. We offer an AS degree and the HT classes are spread out over 2 years. Thank you, hi Sir, I am khalid al-housni from Oman working as histotechnician with bachelor degree working in SQU Hospital since 5 years. I am looking for very good advanced training in histotechnology to reach qualified experience . duration( 4 months ) I hope that you will give me your support and my Job Administration will pay for this training. Thanx alot. yours, Khalid aboelyas@hotmail.com From christiegowan <@t> msn.com Fri Nov 21 15:38:42 2008 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Fri Nov 21 15:38:49 2008 Subject: [Histonet] Sakura tape problem In-Reply-To: Message-ID: Sorry about the name. I meant Cheryl. TGIF ______________________________________________________________ From: "CHRISTIE GOWAN" To: ccrowder@vetmed.lsu.edu, histonet@pathology.swmed.edu Subject: RE: [Histonet] Sakura tape problem Date: Fri, 21 Nov 2008 19:35:49 +0000 > > >______________________________________________________________ > > Cherly, > > I had this same problem a while back with an outside slide >that had > been sent to us for consultation. I even called the company to >get any > input from them. No luck. My final solution was to trim the tape >down > to just the area where the section was located. I sandwiched the >tape > between the glass slide and a glass coverslip using mounting >media > only. I used a paper clamp to secure it until it dried. Do not >use any > xylene. This will cause the tissue to start floating off the >tape and > scatter around. It can be pretty messy so don't use too much >mountant. > Good luck and let us know if you get any better solutions. > > Christie >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Fri Nov 21 15:58:24 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Nov 21 15:58:28 2008 Subject: [Histonet] immuno set-up Message-ID: <582736990811211358k1ec07545od651c94ddbc7160@mail.gmail.com> Hi, For small volumes like that, check with Thermo. They have various sizes of IHC stainers that have different slide capacities. Alternatively you could check out the Shandon Sequenza. I could find more info if you are interested. (BTW: I have no Thermo ties.) Amos Brooks Message: 19 Date: Thu, 20 Nov 2008 20:21:21 +0000 From: zodiac29@comcast.net Subject: [Histonet] immuno set-up To: histonet@lists.utsouthwestern.edu Message-ID: < 112020082021.29733.4925C6C10003F44E000074252214756402C7CD0C0E070B0196@comcast.net > Content-Type: text/plain Hello all, I work at a private lab with one pathologist, and one histotech (me). We do about 5,000 cases a year. We are intrested in doing our immuno's in house (right now we send them out), and wanted to know your opinion on the type of equipment that would be best for this situation. Right now we send about 5 to 10 cases a week out for immuno's. All responses are very much appreciated Jenny From amosbrooks <@t> gmail.com Fri Nov 21 16:06:54 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Nov 21 16:07:00 2008 Subject: [Histonet] RE: Dako and Leica immunostainers Message-ID: <582736990811211406m1dbe2928qe91f7e43752a5e01@mail.gmail.com> No Way!! Just because there are new gadgets & gizmos on the newer instruments doesn't make them better. Versatility = Simplicity! Taking an instrument that works GREAT off the market just because there are newer ones that are all limited is dumb. We should follow Darwin and see the natural selection process through. More complicated systems are often more buggy (... see M$ Vista for example). Bells & Whistles are not selling points. Consistency & Versatility are. Gettin off my soapbox... Amos Message: 23 Date: Thu, 20 Nov 2008 18:35:55 -0500 From: "Sally Price" Subject: [Histonet] RE: Dako and Leica immunostainers To: histonet@lists.utsouthwestern.edu, JCBRITTON@cheshire-med.com, jaustin1967@gmail.com, godsgalnow@aol.com, trathborne@somerset-healthcare.com Message-ID: Content-Type: text/plain; charset=ISO-8859-1 All, After reading this thread I just had offer my comments. I'm not a big fan of systems that do the dewax and AR, primarily because it costs way too much to automate these steps. I've never used the Bond, but I hear that you've gotta put some plastic thingy - that probably costs too much - on top of each slide, you gotta use their detection reagents - which probably cost more than other companies, they charge you for empty barcoded reagent containers, all the slides in the same tray have to use the same detection reagents - which means that the continuous-feed feature has some serious limits, it can't do double-stains, and they have less than 50 IVD-approved antbodies. Can someone verify for me if all this issues are true? If so, why would someone want one of these stainers? The Dako stainer is a dinosaur and with all the newre/better ones available, they should probably take it off the market. Cheers, Sally From joelleweaver <@t> hotmail.com Fri Nov 21 16:07:42 2008 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Nov 21 16:07:47 2008 Subject: [Histonet] Advanced Histo training In-Reply-To: References: Message-ID: I am not aware of any Histology program that can be completed in 4 months. I know that there are a few online programs which offer condensed training and instruction. I am involved with one located in Columbus, Ohio. But this program is 3 quarters in length plus prerequisites. We do offer some non-traditional credit for non-registered, uncertified techs. I can send a link to the program website if this may be of interest -if you will reply to this message. Thanks Joelle > To: histonet@lists.utsouthwestern.edu> From: JMacDonald@mtsac.edu> Date: Fri, 21 Nov 2008 13:04:34 -0800> CC: aboelyas@hotmail.com> Subject: [Histonet] Advanced Histo training> > Is there anyone out there that can answer this gentleman's questions. He > is interested in getting advanced training in histotechnology over a 4 > month period. We offer an AS degree and the HT classes are spread out > over 2 years.> Thank you,> > > hi Sir,> > I am khalid al-housni from Oman working as histotechnician with bachelor > degree working in SQU Hospital since 5 years. I am looking for very good > advanced training in histotechnology to reach qualified experience . > duration( 4 months )> I hope that you will give me your support and my Job Administration will > pay for this training.> > Thanx alot.> > yours,> Khalid> aboelyas@hotmail.com > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Access your email online and on the go with Windows Live Hotmail. http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_access_112008 From victor <@t> pathology.washington.edu Fri Nov 21 16:31:24 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Nov 21 16:31:30 2008 Subject: [Histonet] Advanced Histo training In-Reply-To: References: Message-ID: <492736BC.2040101@pathology.washington.edu> Joelle, I agree that no program can be completed in 4 months, but they are not looking for a complete program. It would be nice to hear exactly what are they looking for with advance training, training with Immunos, special stains, etc.. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. joelle weaver wrote: > I am not aware of any Histology program that can be completed in 4 months. I know that there are a few online programs which offer condensed training and instruction. I am involved with one located in Columbus, Ohio. But this program is 3 quarters in length plus prerequisites. We do offer some non-traditional credit for non-registered, uncertified techs. I can send a link to the program website if this may be of interest -if you will reply to this message. > > Thanks > Joelle > >> To: histonet@lists.utsouthwestern.edu> From: JMacDonald@mtsac.edu> Date: Fri, 21 Nov 2008 13:04:34 -0800> CC: aboelyas@hotmail.com> Subject: [Histonet] Advanced Histo training> > Is there anyone out there that can answer this gentleman's questions. He > is interested in getting advanced training in histotechnology over a 4 > month period. We offer an AS degree and the HT classes are spread out > over 2 years.> Thank you,> > > hi Sir,> > I am khalid al-housni from Oman working as histotechnician with bachelor > degree working in SQU Hospital since 5 years. I am looking for very good > advanced training in histotechnology to reach qualified experience . > duration( 4 months )> I hope that you will give me your support and my Job Administration will > pay for this training.> > Thanx alot.> > yours,> Khalid> aboelyas@hotmail.com > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _________________________________________________________________ > Access your email online and on the go with Windows Live Hotmail. > http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_access_112008_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joelleweaver <@t> hotmail.com Fri Nov 21 16:39:57 2008 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Nov 21 16:40:03 2008 Subject: [Histonet] Advanced Histo training In-Reply-To: <492736BC.2040101@pathology.washington.edu> References: <492736BC.2040101@pathology.washington.edu> Message-ID: Victor Yes, it was kind of hard to tell exactly what he is seeking. He did not really say if he wanted formal instruction or if he wanted to have something more like on-the-job training or something on particular techniques? In any case, I can give him the information that I have, and see if it will help him. Thanks for your input. Histology Program at Columbus State: webpage- http://www.cscc.edu/Histology/index.htm Joelle Weaver> Date: Fri, 21 Nov 2008 14:31:24 -0800> From: victor@pathology.washington.edu> To: joelleweaver@hotmail.com> CC: histonet@lists.utsouthwestern.edu; aboelyas@hotmail.com; jmacdonald@mtsac.edu> Subject: Re: [Histonet] Advanced Histo training> > Joelle,> > I agree that no program can be completed in 4 months, but they are not > looking for a complete program. It would be nice to hear exactly what > are they looking for with advance training, training with Immunos, > special stains, etc..> > Victor> > Victor Tobias> Clinical Applications Analyst> University of Washington Medical Center> Dept of Pathology Room BB220> 1959 NE Pacific> Seattle, WA 98195> victor@pathology.washington.edu> 206-598-2792> 206-598-7659 Fax> =================================================> Privileged, confidential or patient identifiable information may be> contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments.> > > > joelle weaver wrote:> > I am not aware of any Histology program that can be completed in 4 months. I know that there are a few online programs which offer condensed training and instruction. I am involved with one located in Columbus, Ohio. But this program is 3 quarters in length plus prerequisites. We do offer some non-traditional credit for non-registered, uncertified techs. I can send a link to the program website if this may be of interest -if you will reply to this message.> > > > Thanks> > Joelle> > > >> To: histonet@lists.utsouthwestern.edu> From: JMacDonald@mtsac.edu> Date: Fri, 21 Nov 2008 13:04:34 -0800> CC: aboelyas@hotmail.com> Subject: [Histonet] Advanced Histo training> > Is there anyone out there that can answer this gentleman's questions. He > is interested in getting advanced training in histotechnology over a 4 > month period. We offer an AS degree and the HT classes are spread out > over 2 years.> Thank you,> > > hi Sir,> > I am khalid al-housni from Oman working as histotechnician with bachelor > degree working in SQU Hospital since 5 years. I am looking for very good > advanced training in histotechnology to reach qualified experience . > duration( 4 months )> I hope that you will give me your support and my Job Administration will > pay for this training.> > Thanx alot.> > yours,> Khalid> aboelyas@hotmail.com > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> >> > > _________________________________________________________________> > Access your email online and on the go with Windows Live Hotmail.> > http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_access_112008_______________________________________________> > Histonet mailing list> > Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > _________________________________________________________________ Access your email online and on the go with Windows Live Hotmail. http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_access_112008 From ccrowder <@t> vetmed.lsu.edu Fri Nov 21 17:48:46 2008 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Fri Nov 21 17:51:27 2008 Subject: [Histonet] Sakura tape problems Message-ID: Thanks to all of you who have had problems with the Sakura tape. I have received multiple methods to try to save the sections. When I have tried several and combined some I will let you all know what happened so maybe I can help you. Thanks again, Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From slappycraw <@t> yahoo.com Fri Nov 21 19:02:51 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Nov 21 19:02:55 2008 Subject: [Histonet] RE: Dako and Leica immunostainers References: <582736990811211406m1dbe2928qe91f7e43752a5e01@mail.gmail.com> Message-ID: <252650.44730.qm@web53602.mail.re2.yahoo.com> Ditto what Amos related and I will add that the companies making these machines are trying to make a profit first and foremost and not necessarily trying to make things easier for us but I am a huge fan of automation when it gets me from A to B more efficiently, with great results and at a reasonable cost. Larry A. Woody Seattle, Wa. ________________________________ From: Amos Brooks To: sprice2003@gmail.com; "histonet@lists.utsouthwestern.edu" Sent: Friday, November 21, 2008 2:06:54 PM Subject: [Histonet] RE: Dako and Leica immunostainers No Way!! Just because there are new gadgets & gizmos on the newer instruments doesn't make them better. Versatility = Simplicity! Taking an instrument that works GREAT off the market just because there are newer ones that are all limited is dumb. We should follow Darwin and see the natural selection process through. More complicated systems are often more buggy (... see M$ Vista for example). Bells & Whistles are not selling points. Consistency & Versatility are. Gettin off my soapbox... Amos Message: 23 Date: Thu, 20 Nov 2008 18:35:55 -0500 From: "Sally Price" Subject: [Histonet] RE: Dako and Leica immunostainers To: histonet@lists.utsouthwestern.edu, JCBRITTON@cheshire-med.com, jaustin1967@gmail.com, godsgalnow@aol.com, trathborne@somerset-healthcare.com Message-ID: Content-Type: text/plain; charset=ISO-8859-1 All, After reading this thread I just had offer my comments. I'm not a big fan of systems that do the dewax and AR, primarily because it costs way too much to automate these steps. I've never used the Bond, but I hear that you've gotta put some plastic thingy - that probably costs too much - on top of each slide, you gotta use their detection reagents - which probably cost more than other companies, they charge you for empty barcoded reagent containers, all the slides in the same tray have to use the same detection reagents - which means that the continuous-feed feature has some serious limits, it can't do double-stains, and they have less than 50 IVD-approved antbodies. Can someone verify for me if all this issues are true? If so, why would someone want one of these stainers? The Dako stainer is a dinosaur and with all the newre/better ones available, they should probably take it off the market. Cheers, Sally _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Fri Nov 21 21:54:43 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Nov 21 21:54:38 2008 Subject: [Histonet] colon cancer Message-ID: Does anyone know of a type of cancer, either colon or small intestine that is considered hereditary? Jennifer From lpwenk <@t> sbcglobal.net Sat Nov 22 06:39:42 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Nov 22 06:39:57 2008 Subject: [Histonet] Advanced Histo training In-Reply-To: Message-ID: <000901c94c9f$6532f840$0202a8c0@HPPav2> I don't know this person, so I'll answer in general. I get a lot of these types of requests at my school. Most seem to be from the Middle East or Africa. When emailing with them, it mostly appears that they just want to find a quicker way to get into the US. Realize that in most cases, if they are thinking about: - attending a NAACLS accredited college/university based HT/HTL program, they are still going to need to get a student visa first, which can take years. - attending a NAACLS accredited hospital-based HT/HTL program, then a student visa does not work, nor does a work visa. I haven't really found a way to get people from other countries into my hospital-based program (Canada excluded from this comment, as I'm in Michigan and they are literally across the river). - working at a US hospital/lab to do "on-the-job-training", then they need to first get a temporary work visa to enter the US. Or, they need to find a lab that will "sponsor" them, which costs the hospital/lab a lot of money and paperwork. So as you can see, the correct method of getting into the US for histology training is for the person to first get a student visa, work visa or sponsorship. So there's not a lot anyone in a US Histology lab or NAACLS HT/HTL program can do to help someone from another country to get training in the US, if the person doesn't already have a legal way of getting into the US. The other problem is, does training in the US really allow them to take qualification exams in their country? For example, training in another country (excluding Canada) does not allow people to sit for the ASCP HT/HTL exam. For ASCP, the training/experience has to be in a US or Canada histology lab. If the person from another country can get 1 year experience and/or NAACLS program, then they could take the ASCP exam. And then US labs would be more willing to hire them, possibly sponsor them. So this is another route that people are trying to take, to get and stay in the US. Like I said, I don't know this person. Possibly their lab is willing to pay. Maybe they do want to go back to their country with this new knowledge. It might be a legitimate request. But that's not been my experience with these types of requests. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, November 21, 2008 4:05 PM To: histonet@lists.utsouthwestern.edu Cc: aboelyas@hotmail.com Subject: [Histonet] Advanced Histo training Is there anyone out there that can answer this gentleman's questions. He is interested in getting advanced training in histotechnology over a 4 month period. We offer an AS degree and the HT classes are spread out over 2 years. Thank you, hi Sir, I am khalid al-housni from Oman working as histotechnician with bachelor degree working in SQU Hospital since 5 years. I am looking for very good advanced training in histotechnology to reach qualified experience . duration( 4 months ) I hope that you will give me your support and my Job Administration will pay for this training. Thanx alot. yours, Khalid aboelyas@hotmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Nov 22 08:12:27 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Nov 22 08:12:49 2008 Subject: [Histonet] ISH question Message-ID: Hi listmembers, In our lab the unstained IHC-slides are thrown away. Now one slide of each case should be stored in an adequate manner to perform an insitu hybridization if required in the future. I want to store it unstained but deparaffinized and coverslipped. So that it can be filed with the other HE-slides and easily found again. We don't want to store them airsealed in the freezer, and IHC is no issue. Has anybody performed an in situ hybridization on previously coverslipped or even stained tissue? I think, that the DNA would be well preserved. What could be a cause of DNA-degradation in this conservation-manner? Thanks for sharing your experiences. Gudrun Lang Histolab, Akh Linz, Austria From steely0511 <@t> yahoo.com Sat Nov 22 16:43:26 2008 From: steely0511 <@t> yahoo.com (John Steel) Date: Sat Nov 22 16:43:30 2008 Subject: [Histonet] RE: Dako and Leica immunostainers References: <582736990811211406m1dbe2928qe91f7e43752a5e01@mail.gmail.com> Message-ID: <503969.33825.qm@web59703.mail.ac4.yahoo.com> Perhaps, you need to ask this professional's level of comfort in performing IHC... Best regards, Jerry ________________________________ From: Amos Brooks To: sprice2003@gmail.com; "histonet@lists.utsouthwestern.edu" Sent: Friday, November 21, 2008 5:06:54 PM Subject: [Histonet] RE: Dako and Leica immunostainers No Way!! ? Just because there are new gadgets & gizmos on the newer instruments doesn't make them better. Versatility = Simplicity! Taking an instrument that works GREAT off the market just because there are newer ones that are all limited is dumb. We should follow Darwin and see the natural selection process through. More complicated systems are often more buggy (... see M$ Vista for example). Bells & Whistles are not selling points. Consistency & Versatility are. Gettin off my soapbox... Amos Message: 23 Date: Thu, 20 Nov 2008 18:35:55 -0500 From: "Sally Price" Subject: [Histonet] RE: Dako and Leica immunostainers To: histonet@lists.utsouthwestern.edu, JCBRITTON@cheshire-med.com, ? ? ? jaustin1967@gmail.com, godsgalnow@aol.com, ? ? ? trathborne@somerset-healthcare.com Message-ID: ? ? ? Content-Type: text/plain; charset=ISO-8859-1 All, After reading this thread I just had offer my comments.? I'm not a big fan of systems that do the dewax and AR, primarily because it costs way too much to automate these steps.? I've never used the Bond, but I hear that you've gotta put some plastic thingy - that probably costs too much - on top of each slide, you gotta use their detection reagents - which probably cost more than other companies, they charge you for empty barcoded reagent containers, all the slides in the same tray have to use the same detection reagents - which means that the continuous-feed feature has some serious limits, it can't do double-stains, and they have less than 50 IVD-approved antbodies.? Can someone verify for me if all this issues are true?? If so, why would someone want one of these stainers?? The Dako stainer is a dinosaur and with all the newre/better ones available, they should probably take it off the market. Cheers, Sally _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sccrshlly <@t> yahoo.com Sat Nov 22 16:51:35 2008 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Sat Nov 22 16:51:43 2008 Subject: [Histonet] Re: Colon Cancer Message-ID: <700569.2312.qm@web90302.mail.mud.yahoo.com> I work with many GI biopsies and occassionally we send off?slides for testing for MSI (Microsattelite Instability).??This is a test for Hereditary Nonpolyposis Colorectal Cancer (HNPCC), also known as Lynch Sydrome.? The IHC tests for normal gene expression. You can "google" HNPCC or MSI and find several informative sites. ? Hope this helps, ? Shelly From slappycraw <@t> yahoo.com Sat Nov 22 17:01:01 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Sat Nov 22 17:01:05 2008 Subject: [Histonet] RE: Dako and Leica immunostainers References: <582736990811211406m1dbe2928qe91f7e43752a5e01@mail.gmail.com> <503969.33825.qm@web59703.mail.ac4.yahoo.com> Message-ID: <965006.73541.qm@web53607.mail.re2.yahoo.com> Dako doesn't even manufacture an IHC stainer unless you count the Eridan which was a failure. The instrument that they distribute is manufactured by Lab Vision/ now Thermo Shandon and hardly a dinosaur. If a person doesn't know the theory behind doing IHC or can't perform IHC on the bench then they really have no business doing IHC by any machine. I don't care how idiot proof they make an instrument there are still times when you have to improvise especially in the research field which requires a more open type platform. In research we do so many different types antibodies with everything from A-Z when it comes to AR, and methodology that an open platform is essential. The idiot proof machines have a niche but it's not in research. Larry A. Woody Seattle, Wa. ________________________________ From: John Steel To: Amos Brooks ; sprice2003@gmail.com; "histonet@lists.utsouthwestern.edu" Sent: Saturday, November 22, 2008 2:43:26 PM Subject: Re: [Histonet] RE: Dako and Leica immunostainers Perhaps, you need to ask this professional's level of comfort in performing IHC... Best regards, Jerry ________________________________ From: Amos Brooks To: sprice2003@gmail.com; "histonet@lists.utsouthwestern.edu" Sent: Friday, November 21, 2008 5:06:54 PM Subject: [Histonet] RE: Dako and Leica immunostainers No Way!! Just because there are new gadgets & gizmos on the newer instruments doesn't make them better. Versatility = Simplicity! Taking an instrument that works GREAT off the market just because there are newer ones that are all limited is dumb. We should follow Darwin and see the natural selection process through. More complicated systems are often more buggy (... see M$ Vista for example). Bells & Whistles are not selling points. Consistency & Versatility are. Gettin off my soapbox... Amos Message: 23 Date: Thu, 20 Nov 2008 18:35:55 -0500 From: "Sally Price" Subject: [Histonet] RE: Dako and Leica immunostainers To: histonet@lists.utsouthwestern.edu, JCBRITTON@cheshire-med.com, jaustin1967@gmail.com, godsgalnow@aol.com, trathborne@somerset-healthcare.com Message-ID: Content-Type: text/plain; charset=ISO-8859-1 All, After reading this thread I just had offer my comments. I'm not a big fan of systems that do the dewax and AR, primarily because it costs way too much to automate these steps. I've never used the Bond, but I hear that you've gotta put some plastic thingy - that probably costs too much - on top of each slide, you gotta use their detection reagents - which probably cost more than other companies, they charge you for empty barcoded reagent containers, all the slides in the same tray have to use the same detection reagents - which means that the continuous-feed feature has some serious limits, it can't do double-stains, and they have less than 50 IVD-approved antbodies. Can someone verify for me if all this issues are true? If so, why would someone want one of these stainers? The Dako stainer is a dinosaur and with all the newre/better ones available, they should probably take it off the market. Cheers, Sally _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From steely0511 <@t> yahoo.com Sat Nov 22 17:42:59 2008 From: steely0511 <@t> yahoo.com (John Steel) Date: Sat Nov 22 17:43:04 2008 Subject: [Histonet] RE: Dako and Leica immunostainers References: <582736990811211406m1dbe2928qe91f7e43752a5e01@mail.gmail.com> <503969.33825.qm@web59703.mail.ac4.yahoo.com> <965006.73541.qm@web53607.mail.re2.yahoo.com> Message-ID: <230893.94746.qm@web59716.mail.ac4.yahoo.com> The Dako Eridan was never released, thus is a moot point, and agree that the current open platform Dako offers is very flexible for a variety of uses - with a caveat.? Different instruments require different levels of IHC experience, IHC confort level, and who pre-screens stained slides prior to passing on to the pathologist or PI.? There are many out in histo-land that perform and interpret IHC that are well qualified to do so - and there are others that are not.? There is NO instrument on the market that is capable of screening clinical assays - no vendor is there yet.? Would you want a nonCLIA/CAP approved lab to diagnose your family's specimen?? The certifications are in place for a reason.? Granted, research is different, although would you bet your grant submission or renewal on an untested improvisation???? It is called research for a reason, but I believe consistancy, reproducibility, and reliability is the hallmark of research and IHC. Best regards, Jerry ________________________________ From: Larry Woody To: John Steel ; Amos Brooks ; sprice2003@gmail.com; "histonet@lists.utsouthwestern.edu" Sent: Saturday, November 22, 2008 6:01:01 PM Subject: Re: [Histonet] RE: Dako and Leica immunostainers Dako doesn't even manufacture an IHC stainer unless you count the Eridan which was a failure. The instrument that they distribute is manufactured by Lab Vision/ now Thermo Shandon and hardly a dinosaur. If a person doesn't know the theory behind doing IHC or can't perform IHC on the bench then they really have no business doing IHC by any machine. I don't care how idiot proof they make an instrument there are still times when you have to improvise especially in the research field which requires a more open type platform. In research we do so many different types antibodies with everything from A-Z when it comes to AR, and methodology that an open platform is essential. The idiot proof machines have a niche but it's not in research. Larry A. Woody Seattle, Wa. ________________________________ From: John Steel To: Amos Brooks ; sprice2003@gmail.com; "histonet@lists.utsouthwestern.edu" Sent: Saturday, November 22, 2008 2:43:26 PM Subject: Re: [Histonet] RE: Dako and Leica immunostainers Perhaps, you need to ask this professional's level of comfort in performing IHC... Best regards, Jerry ________________________________ From: Amos Brooks To: sprice2003@gmail.com; "histonet@lists.utsouthwestern.edu" Sent: Friday, November 21, 2008 5:06:54 PM Subject: [Histonet] RE: Dako and Leica immunostainers No Way!! ? Just because there are new gadgets & gizmos on the newer instruments doesn't make them better. Versatility = Simplicity! Taking an instrument that works GREAT off the market just because there are newer ones that are all limited is dumb. We should follow Darwin and see the natural selection process through. More complicated systems are often more buggy (... see M$ Vista for example). Bells & Whistles are not selling points. Consistency & Versatility are. Gettin off my soapbox... Amos Message: 23 Date: Thu, 20 Nov 2008 18:35:55 -0500 From: "Sally Price" Subject: [Histonet] RE: Dako and Leica immunostainers To: histonet@lists.utsouthwestern.edu, JCBRITTON@cheshire-med.com, ? ? ? jaustin1967@gmail.com, godsgalnow@aol.com, ? ? ? trathborne@somerset-healthcare.com Message-ID: ? ? ? Content-Type: text/plain; charset=ISO-8859-1 All, After reading this thread I just had offer my comments.? I'm not a big fan of systems that do the dewax and AR, primarily because it costs way too much to automate these steps.? I've never used the Bond, but I hear that you've gotta put some plastic thingy - that probably costs too much - on top of each slide, you gotta use their detection reagents - which probably cost more than other companies, they charge you for empty barcoded reagent containers, all the slides in the same tray have to use the same detection reagents - which means that the continuous-feed feature has some serious limits, it can't do double-stains, and they have less than 50 IVD-approved antbodies.? Can someone verify for me if all this issues are true?? If so, why would someone want one of these stainers?? The Dako stainer is a dinosaur and with all the newre/better ones available, they should probably take it off the market. Cheers, Sally _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Sat Nov 22 18:09:32 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Sat Nov 22 18:09:36 2008 Subject: [Histonet] RE: Dako and Leica immunostainers References: <582736990811211406m1dbe2928qe91f7e43752a5e01@mail.gmail.com> <503969.33825.qm@web59703.mail.ac4.yahoo.com> <965006.73541.qm@web53607.mail.re2.yahoo.com> <230893.94746.qm@web59716.mail.ac4.yahoo.com> Message-ID: <453122.49029.qm@web53606.mail.re2.yahoo.com> The Eridan was sold to a couple of labs right here in Seattle as well as a few others out of state I know of but it really doesn't matter since it was a failure. As far as grants and renewals go there are plenty of companies doing research that don't rely on grants or loans. Just because a lab is CLIA/CAP approved doesn't mean there are competent people working there. The public is completely unaware of the minimal qualifications it takes to handle specimens in clinical labs and everyone knows what I mean. In research a failed experiment can tell us as much as one that worked and by improvise I simply mean that when something is staining or not staining the way you would like it or expect it to a person should be able to devise several methods that might correct the problem, something a machine cannot do yet. Larry A. Woody Seattle, Wa. ________________________________ From: John Steel To: Larry Woody ; Amos Brooks ; sprice2003@gmail.com; "histonet@lists.utsouthwestern.edu" Sent: Saturday, November 22, 2008 3:42:59 PM Subject: Re: [Histonet] RE: Dako and Leica immunostainers The Dako Eridan was never released, thus is a moot point, and agree that the current open platform Dako offers is very flexible for a variety of uses - with a caveat. Different instruments require different levels of IHC experience, IHC confort level, and who pre-screens stained slides prior to passing on to the pathologist or PI. There are many out in histo-land that perform and interpret IHC that are well qualified to do so - and there are others that are not. There is NO instrument on the market that is capable of screening clinical assays - no vendor is there yet. Would you want a nonCLIA/CAP approved lab to diagnose your family's specimen? The certifications are in place for a reason. Granted, research is different, although would you bet your grant submission or renewal on an untested improvisation??? It is called research for a reason, but I believe consistancy, reproducibility, and reliability is the hallmark of research and IHC. Best regards, Jerry ________________________________ From: Larry Woody To: John Steel ; Amos Brooks ; sprice2003@gmail.com; "histonet@lists.utsouthwestern.edu" Sent: Saturday, November 22, 2008 6:01:01 PM Subject: Re: [Histonet] RE: Dako and Leica immunostainers Dako doesn't even manufacture an IHC stainer unless you count the Eridan which was a failure. The instrument that they distribute is manufactured by Lab Vision/ now Thermo Shandon and hardly a dinosaur. If a person doesn't know the theory behind doing IHC or can't perform IHC on the bench then they really have no business doing IHC by any machine. I don't care how idiot proof they make an instrument there are still times when you have to improvise especially in the research field which requires a more open type platform. In research we do so many different types antibodies with everything from A-Z when it comes to AR, and methodology that an open platform is essential. The idiot proof machines have a niche but it's not in research. Larry A. Woody Seattle, Wa. ________________________________ From: John Steel To: Amos Brooks ; sprice2003@gmail.com; "histonet@lists.utsouthwestern.edu" Sent: Saturday, November 22, 2008 2:43:26 PM Subject: Re: [Histonet] RE: Dako and Leica immunostainers Perhaps, you need to ask this professional's level of comfort in performing IHC... Best regards, Jerry ________________________________ From: Amos Brooks To: sprice2003@gmail.com; "histonet@lists.utsouthwestern.edu" Sent: Friday, November 21, 2008 5:06:54 PM Subject: [Histonet] RE: Dako and Leica immunostainers No Way!! Just because there are new gadgets & gizmos on the newer instruments doesn't make them better. Versatility = Simplicity! Taking an instrument that works GREAT off the market just because there are newer ones that are all limited is dumb. We should follow Darwin and see the natural selection process through. More complicated systems are often more buggy (... see M$ Vista for example). Bells & Whistles are not selling points. Consistency & Versatility are. Gettin off my soapbox... Amos Message: 23 Date: Thu, 20 Nov 2008 18:35:55 -0500 From: "Sally Price" Subject: [Histonet] RE: Dako and Leica immunostainers To: histonet@lists.utsouthwestern.edu, JCBRITTON@cheshire-med.com, jaustin1967@gmail.com, godsgalnow@aol.com, trathborne@somerset-healthcare.com Message-ID: Content-Type: text/plain; charset=ISO-8859-1 All, After reading this thread I just had offer my comments. I'm not a big fan of systems that do the dewax and AR, primarily because it costs way too much to automate these steps. I've never used the Bond, but I hear that you've gotta put some plastic thingy - that probably costs too much - on top of each slide, you gotta use their detection reagents - which probably cost more than other companies, they charge you for empty barcoded reagent containers, all the slides in the same tray have to use the same detection reagents - which means that the continuous-feed feature has some serious limits, it can't do double-stains, and they have less than 50 IVD-approved antbodies. Can someone verify for me if all this issues are true? If so, why would someone want one of these stainers? The Dako stainer is a dinosaur and with all the newre/better ones available, they should probably take it off the market. Cheers, Sally _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Sat Nov 22 22:24:47 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Nov 22 22:24:51 2008 Subject: [Histonet] Test only #1. Don't bother to read or reply. Message-ID: Some of my recent posts to the Histonet Listserver have bee garbled, although other emails that I send are OK. These few lines are sent as plain text to see if they look OK when distributed to the List. This is a second paragraph. John K. = = = From jkiernan <@t> uwo.ca Sat Nov 22 22:27:16 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Nov 22 22:27:20 2008 Subject: [Histonet] Test message #2. Don't bother to read it. Message-ID: Some of my recent posts to the Histonet Listserver have bee garbled, although other emails that I send are OK. These few lines are sent as rich (HTML) text (HTML) to see if they look OK when distributed to the List. This is a second paragraph. John K. = = = From jkiernan <@t> uwo.ca Sat Nov 22 23:08:11 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Nov 22 23:08:18 2008 Subject: [Histonet] Stain for Cellulose In-Reply-To: References: <200811201548.mAKFmoIM001492@ch1ssaenov02.novartis.com> Message-ID: Cellulose isn't a "mammalian tisssue element", but there's usually some to be seen in the lumen of the alimentary tract, especially stomach and colon, of rats, mice etc. Cellulose is PAS-positive. It's also birefringent (if you have a microscope with polarizing filters), but mostly it shows up because it's obviously present as plant cell walls. These persist long after the death and digestion of the once living parts of the cells, and they generally contain carbohydrates other than cellulose, along with various polyphenols etc that stain with cationic (basic) dyes and with various aluminium- and iron-haematoxylin methods. Clark's "Staining Procedures" (3rd ed, 1973) has 9 pages of staining methods for cellulose cell walls. Ruzin's "Plant Microtechnique and Microscopy" (1999) gives only the PAS method as a stain (not specific, of course) for cellulose. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: thomas.crowell@novartis.com Date: Thursday, November 20, 2008 11:37 Subject: [Histonet] Stain for Cellulose To: histonet@lists.utsouthwestern.edu > I am looking for a stain that will specifically label cellulose, > mixed in > with other mammalian tisssue elements. Any ideas? > > Tom Crowell > Novartis Institute for BiomedicalReseach > Cambridge, MA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From napoli <@t> siscom.net Sun Nov 23 09:36:13 2008 From: napoli <@t> siscom.net (napoli@siscom.net) Date: Sun Nov 23 09:36:18 2008 Subject: [Histonet] malachite green counterstain Message-ID: <4929786d.28.4b16.785945809@siscom.net> Anyone have knowledge of using malachite green as a counterstain for PAS stains for fungus? I have been getting poor results using it as it tends to wash out very quickly in the dehydrating process before coverslipping. Any ideas? It seems as though it hasn't worked as well lately as it has in the past...getting mixed successes. Something is wrong with my voodoo! Puzzled dermatotech From RSRICHMOND <@t> aol.com Sun Nov 23 17:35:31 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Sun Nov 23 17:35:35 2008 Subject: [Histonet] Re: malachite green counterstain Message-ID: An anonymous query: >>Anyone have knowledge of using malachite green as a counterstain for PAS stains for fungus?<< The usual green counterstain for red stains such as PAS is fast green FCF. Some pathologists prefer a hematoxylin counterstain for PAS - it may be worth asking. Bob Richmond Samurai Pathologist Knoxville TN From RSRICHMOND <@t> aol.com Sun Nov 23 17:42:19 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Sun Nov 23 17:42:24 2008 Subject: [Histonet] Re: Colon cancer Message-ID: Probably 5 to 10% of colon cancers appear to be genetically inherited, a number that's likely to increase. Familial colonic polyposis is the best known of these inherited tumors, with almost all affected individuals getting colon cancer at an early age. Hereditary nonpolyposis cancer is more recently described, and there are several other inherited tumor syndromes that increase the risk of colon cancer, including Lynch syndrome 2. The use of tests such as microsatellite instability and k-ras remains somewhat controversial, but we may expect more requests for immunohistochemical or molecular tests for hereditary colon cancer, particularly for patients diagnosed with colon cancer at an early age. Bob Richmond Samurai Pathologist Knoxville TN From jkiernan <@t> uwo.ca Mon Nov 24 00:40:19 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Nov 24 00:40:23 2008 Subject: [Histonet] malachite green counterstain In-Reply-To: <4929786d.28.4b16.785945809@siscom.net> References: <4929786d.28.4b16.785945809@siscom.net> Message-ID: Check the original source of your method. If it's a typed sheet with forget is used applications are summar Biological Stains. follow PAS, I recommend any ha Mayers's, Ehrlich's, Gill's) followed green FCF (0.1-0.2% in 1-2% acetic acid). T blue nuclei and green cytoplasm and collagen. The green bluish cast, but both colours contrast well with PAS. For a wi variety of anionic counterstains in various colours, see Presnell J K & Schreibman MP (1997) Humason's Animal Tissue Techniques, Earlier editions of information.
 
John K iernan
Anatomy, UWO
London, Canada
- - -
"napoli@siscom.net" <napoli@siscom.net>
Date: Sunday, November malachite green coun histonet@lists.utsouthwestern.edu
< Anyone have knowledge of using malachite green as a
counterstain for PAS stains for fungus? I have been getting< BR>> poor results using it as it tends to wash out very quickly coverslipping. Any hasn't worked as well l past...getting mixed succes wrong
> with my voodoo!
>
> Puzzled dermatotech
>
> < ______________ _______________________ 5F list
Histonet@lists.utsouthwestern.edu
> http: //lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Mon Nov 24 03:44:23 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Nov 24 03:44:31 2008 Subject: [Histonet] RE: Floaters In-Reply-To: <8839B08E3ED7364E8CBBD53882C984D50994CD57@MAILSRV01.midmichigan.net> References: <8839B08E3ED7364E8CBBD53882C984D50994CD57@MAILSRV01.midmichigan.net> Message-ID: <7722595275A4DD4FA225B92CDBF174A1744E1B4638@EXC-MBX3.cfs.le.ac.uk> I tend to try and poke them down with a metal coat hanger!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri.Hallada@midmichigan.org Sent: 21 November 2008 18:17 To: histonet@pathology.swmed.edu Subject: [Histonet] Floaters Would anyone care to share their policy on floaters? Also, does anyone know if there is a CAP policy in floaters? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lyork <@t> kwbpathology.com Mon Nov 24 06:39:54 2008 From: lyork <@t> kwbpathology.com (LYork) Date: Mon Nov 24 06:40:15 2008 Subject: [Histonet] Cryostats Message-ID: <000001c94e31$c81c4630$8e01a8c0@KWBPA.local> Can anyone provide information on portable cryostats? We need one that will handle only 4-5 frozens per week. From histonetalias <@t> gmail.com Mon Nov 24 08:08:32 2008 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Mon Nov 24 08:08:36 2008 Subject: [Histonet] RE: Floaters In-Reply-To: <7722595275A4DD4FA225B92CDBF174A1744E1B4638@EXC-MBX3.cfs.le.ac.uk> References: <8839B08E3ED7364E8CBBD53882C984D50994CD57@MAILSRV01.midmichigan.net> <7722595275A4DD4FA225B92CDBF174A1744E1B4638@EXC-MBX3.cfs.le.ac.uk> Message-ID: <4b6c85510811240608j58c1ef18p11040b9818e8fe3a@mail.gmail.com> ANP.23350 Phase II N/A YES NO *Are flotation baths clean and well-maintained, and is there a procedure for preventing cross-contamination of paraffin sections in the bath?* * * NOTE: Of particular importance are periodic water changes or blotting of the water surface so that sections from one patient block are not inadvertently carried over to another case (so-called "floaters" or "extraneous tissue"). COMMENTARY: On Mon, Nov 24, 2008 at 4:44 AM, Edwards, R.E. wrote: > I tend to try and poke them down with a metal coat hanger!. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Teri.Hallada@midmichigan.org > Sent: 21 November 2008 18:17 > To: histonet@pathology.swmed.edu > Subject: [Histonet] Floaters > > Would anyone care to share their policy on floaters? Also, does anyone > know if there is a CAP policy in floaters? > > Teresa Hallada BS, MT/CT (ASCP) > Lead Cytotechnologist > MidMichigan Health - Gratiot > teri.hallada@midmichigan.org > 989.463.1101 ext 3423 > > Please note that this email message and any attachments may contain > privileged and confidential information that is protected against use or > disclosure under federal and state law. The information is intended only > for the personal and confidential use of the intended recipient. If the > reader of this message is not the intended recipient or the employee or > agent responsible for delivering it to the intended recipient, you are > hereby notified that you have received this information in error and that > any review, dissemination, distribution, copying or action taken in reliance > on the contents of this communication is strictly prohibited. If you have > received this email in error, please advise by immediate reply. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- The Unknown HT(ASCP) From vazquezr <@t> ohsu.edu Mon Nov 24 08:21:17 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon Nov 24 08:21:41 2008 Subject: [Histonet] RE: Floaters In-Reply-To: <4b6c85510811240608j58c1ef18p11040b9818e8fe3a@mail.gmail.com> References: <8839B08E3ED7364E8CBBD53882C984D50994CD57@MAILSRV01.midmichigan.net> <7722595275A4DD4FA225B92CDBF174A1744E1B4638@EXC-MBX3.cfs.le.ac.uk> <4b6c85510811240608j58c1ef18p11040b9818e8fe3a@mail.gmail.com> Message-ID: <2A582E8156B45F468A62D1F1D20AF083489F71@EX-BE08.ohsu.edu> Rope and a cinder block??!! Happy Monday! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histonet Alias Sent: Monday, November 24, 2008 6:09 AM To: Edwards, R.E. Cc: Teri.Hallada@midmichigan.org; histonet@pathology.swmed.edu Subject: Re: [Histonet] RE: Floaters ANP.23350 Phase II N/A YES NO *Are flotation baths clean and well-maintained, and is there a procedure for preventing cross-contamination of paraffin sections in the bath?* * * NOTE: Of particular importance are periodic water changes or blotting of the water surface so that sections from one patient block are not inadvertently carried over to another case (so-called "floaters" or "extraneous tissue"). COMMENTARY: On Mon, Nov 24, 2008 at 4:44 AM, Edwards, R.E. wrote: > I tend to try and poke them down with a metal coat hanger!. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Teri.Hallada@midmichigan.org > Sent: 21 November 2008 18:17 > To: histonet@pathology.swmed.edu > Subject: [Histonet] Floaters > > Would anyone care to share their policy on floaters? Also, does anyone > know if there is a CAP policy in floaters? > > Teresa Hallada BS, MT/CT (ASCP) > Lead Cytotechnologist > MidMichigan Health - Gratiot > teri.hallada@midmichigan.org > 989.463.1101 ext 3423 > > Please note that this email message and any attachments may contain > privileged and confidential information that is protected against use or > disclosure under federal and state law. The information is intended only > for the personal and confidential use of the intended recipient. If the > reader of this message is not the intended recipient or the employee or > agent responsible for delivering it to the intended recipient, you are > hereby notified that you have received this information in error and that > any review, dissemination, distribution, copying or action taken in reliance > on the contents of this communication is strictly prohibited. If you have > received this email in error, please advise by immediate reply. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- The Unknown HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akelly <@t> roseliassociates.com Mon Nov 24 08:35:26 2008 From: akelly <@t> roseliassociates.com (Dr. Andrea Kelly) Date: Mon Nov 24 08:35:42 2008 Subject: [Histonet] Biospecimen Research Symposium Message-ID: <492ABBAE.3070707@roseliassociates.com> *REGISTER TODAY !* Registration is now open for the National Cancer Institute's 2^nd Annual Biospecimen Research Network Symposium , "Advancing Cancer Research Through Biospecimen Science," March 16-18, 2009 in Bethesda, Maryland. The symposium will address the significant impact of pre-analytical biospecimen variables on cancer research and molecular medicine. Hosted by NCI's Office of Biorepositories and Biospecimen Research , the meeting will bring together leaders in the fields of biospecimen research, genomics, proteomics, oncology, pathology, biobanking, hospital administration and pharmaceutics as well as patient advocates. *Location*: Bethesda North Marriott Hotel & Conference Center 5701 Marinelli Road Bethesda, Maryland *Sessions include*: * Using the right biospecimens in clinical research and care * Evidence for pre-analytical variability during the acquisition and processing of tissue biospecimens * Solutions for reducing pre-analytical variability in biofluid-based biomarker R&D * Considerations for experimental design in biospecimen research * Strategies and tools for accessing and publishing data in biospecimen science * Evidence for the proper use of previously banked tissues * The patient's perspective on research in the biospecimen sciences Please join us for this stimulating and informative three-day event that will feature presentations, interactive discussions and workshops about the issue of biospecimen quality and ways to address it. Register now to reserve your space! From relia1 <@t> earthlink.net Mon Nov 24 08:39:58 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Nov 24 08:40:02 2008 Subject: [Histonet] RELIA Histology Careers Bulletin for 11-30 and A Few Holiday Shopping Tips!! Message-ID: Hi Histonetters! I hope you are having a wonderful Fall. The weather is getting cooler and the Holidays are right around the corner. I realize that at this time of year a job change is the furthest thing from most people?s minds but I have some great opportunities that I wanted to share along with some great tips for holiday shopping. All of my positions are full time permanent positions with premier companies who offer excellent salaries, benefits and relocation assistance. Most of them are willing to look at onsite interviews and start dates after the holidays so if you or anyone you know might be looking now or after the first of the year it wouldn?t hurt to shoot me a quick e-mail at relia1@earthlink.net or give me a quick call toll free at 866-607-3542. Currently I have management positions in CA, NH, OH and TX. I also have tech positions in CA, TX, WA, MD and MA. Holiday shopping is always fun, crazy, chaotic, inspiring, and challenging. Here are some tips that might save you some time, money and stress. Black Friday is the day after Thanksgiving the official first day of Holiday Shopping. If you want a heads up on the advertisements from your favorite stores go to the website: www.bfads.net They have a lot of the ads posted on their site NOW that will be in your newspaper on Thanksgiving morning. The Monday after Thanksgiving is known as Cyber Monday it is the busiest online shopping day of the year and there is another website that will give you heads up on deals for Cyber Monday. This website is www.cybermonday.com The best part of this site is that the stores that advertise on their site donate a percentage of sales to a scholarship fund. Again if you or anyone you know is interested in a new opportunity now or after the holidays get in touch with me. There are a lot of recruiters out there but remember I am the only one with the experience, connections and respect for you and your career that specializes exclusively in histology. I want to place you in a new position only if it is the right place, right time and right position for you. And I am always available for career advice, resume assistance or just a chat. Let me be your career advocate. Happy Holidays!!! Pam 866-607-3542 Relia1@earthlink.net Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net < mailto:relia1@earthlink.net> << http://home.earthlink.net/~relia1>> www.myspace.com/pamatrelia < http://www.myspace.com/pamatrelia> From CareerStudio <@t> aol.com Mon Nov 24 09:17:54 2008 From: CareerStudio <@t> aol.com (CareerStudio@aol.com) Date: Mon Nov 24 09:17:32 2008 Subject: [Histonet] Career Opening: Histology Manager with leading lab in Dallas, TX Message-ID: Currently a Histology Manager role is open with a leading, full-service clinical reference laboratory in Dallas, TX. Reporting to the Laboratory Director, accountabilities will include: - Management of all technical and operational activities of the Histology Department, including staffing, planning, coordination and evaluation - Troubleshooting quality and production issues including stains, processes, systems, and equipment. - Developing and implementing improvements in systems and processes for enhanced efficiency and quality, while managing growth. 8+ years of experience in histology with at least 5 years of supervisory experience is required for this position, with demonstrated management ability, organizational and training skills. Must have HT (ASCP) certification, minimum of Associates degree, excellent written and oral communication skills, as well as Microsoft Office and database management background. Our client is offering attractive base salary commensurate with experience, and relocation assistance is available. We are a part of a national search firm focused on the laboratory and biotechnology sectors. Our clients nationwide are premier laboratories with the latest in equipment and teams of valued professionals. Please contact Barbara Siegel at careerstudio@aol.com if you are interested in an opportunity in this fine city. Career Studio national search Palm Beach, FL careerstudio@aol.com 561-738-6363 ************** One site has it all. Your email accounts, your social networks, and the things you love. Try the new AOL.com today!(http://pr.atwola.com/promoclk/100000075x1212962939x1200825291/aol?redir=http://www.aol.com/?optin=new-dp %26icid=aolcom40vanity%26ncid=emlcntaolcom00000001) From Stephen.Eyres <@t> sanofi-aventis.com Mon Nov 24 09:37:39 2008 From: Stephen.Eyres <@t> sanofi-aventis.com (Stephen.Eyres@sanofi-aventis.com) Date: Mon Nov 24 09:37:46 2008 Subject: [Histonet] Leica slide writer - problems?? In-Reply-To: Message-ID: <90B6684A9D6DAF468F7A5DC148754E1DC21C00@ALPW31.f2.enterprise> Hi Joyce, Was this a recent problem, and has it been resolved? Thanks Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Thursday, November 20, 2008 6:20 PM To: Histonet Subject: RE: [Histonet] Leica slide writer - problems?? We have had the same problems, we discovered the slide surface on some of the snowcoat slides changed to a slick surface instead of a rough surface. We have switched to a pale gray slide that still has the rough surface and we have not had any printing wipe off. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara.Lees@sanofi-aventis.com Sent: Thursday, November 20, 2008 7:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica slide writer - problems?? Hi, Has anyone else been having problems with there Lieca slide writer. Our problems mainly involve the ink writing rubbing off before and after staining!!, we use Surgipath snowcoated slides, what does everyone else use as they have hinted it could be our slides. Any help would be fantastic. Thanks Loads Sara _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Mon Nov 24 11:06:59 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Nov 24 11:07:21 2008 Subject: [Histonet] Iron stains Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E05F@lmhsmail.lmhealth.org> We may be having a problem with our iron stains. I say that we 'may' be having a problem because our positive control always works. For bone marrows, we stain the core, aspirate, a smear, and a purchased positive control. The stain is all over the place. We may have a loaded smear with a negative core and aspirate. We may have a positive aspirate with a negative core and smear. We may have all negatives even though clinically, there should be iron. Our control always works. The pathologists do not trust the stain and really do not believe the results. I have stood by the stain due to the positive control but I find it increasingly difficult. I would appreciate any thoughts. Our procedures are outlined below... The aspirate is allowed to clot before placing it into 10%NBF. The core is placed briefly into DecalStat (until it floats) then placed into 10%NBF. Both are then processed with our regular tissues. The spend anywhere from 4 to 10 hours in formalin. The following day, they are cut and along with one of the purchased controls, run down to water on the stainer. (8 mins onboard oven, americlear, alcohols, water) The smear is placed into 95% alcohol then rinsed in distilled. All slides are then stained using Perl's Method for iron pigment. We have already explored the way the specimens are collected during the BM procedure, and decal solution. All reagents are good. I'm wondering about the formalin fixation. I know that threre are other probably better fixatives. Does anybody use another fixative prior to formalin? For those using formalin only, do you limit the fixation time in any way? I really would appreciate any comments. Such a common and simple stain. We should be able to trust it. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org From rjbuesa <@t> yahoo.com Mon Nov 24 11:27:02 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 24 11:27:08 2008 Subject: [Histonet] Iron stains In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E05F@lmhsmail.lmhealth.org> Message-ID: <32860.84420.qm@web65711.mail.ac4.yahoo.com> If your controls always work, at least you can be sure that your Perl's reagents are in good condition. Your method differs from what I used in the following: 1- the aspirate and the core were placed immediately into NBF that was pH corrected to 7.0 exactly. When received in the laboratory the?core Bx?was?placed in EDTA to decalcify. You decalcify first and that is not recommendable because fixation should precede decalcification, perhaps here you have a source of inconsistency depending on the characteristics of the core Bx. 2- the smears were allowed to air dry and fixed with methanol and stained afterward. 3- processing was similar to what you describe (overnight) to be sectioned early morning next day to stain, etc. Decalcifying before fixing could be a cause of problem. Ren? J. --- On Mon, 11/24/08, Tom McNemar wrote: From: Tom McNemar Subject: [Histonet] Iron stains To: histonet@pathology.swmed.edu Date: Monday, November 24, 2008, 12:06 PM We may be having a problem with our iron stains. I say that we 'may' be having a problem because our positive control always works. For bone marrows, we stain the core, aspirate, a smear, and a purchased positive control. The stain is all over the place. We may have a loaded smear with a negative core and aspirate. We may have a positive aspirate with a negative core and smear. We may have all negatives even though clinically, there should be iron. Our control always works. The pathologists do not trust the stain and really do not believe the results. I have stood by the stain due to the positive control but I find it increasingly difficult. I would appreciate any thoughts. Our procedures are outlined below... The aspirate is allowed to clot before placing it into 10%NBF. The core is placed briefly into DecalStat (until it floats) then placed into 10%NBF. Both are then processed with our regular tissues. The spend anywhere from 4 to 10 hours in formalin. The following day, they are cut and along with one of the purchased controls, run down to water on the stainer. (8 mins onboard oven, americlear, alcohols, water) The smear is placed into 95% alcohol then rinsed in distilled. All slides are then stained using Perl's Method for iron pigment. We have already explored the way the specimens are collected during the BM procedure, and decal solution. All reagents are good. I'm wondering about the formalin fixation. I know that threre are other probably better fixatives. Does anybody use another fixative prior to formalin? For those using formalin only, do you limit the fixation time in any way? I really would appreciate any comments. Such a common and simple stain. We should be able to trust it. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Mon Nov 24 11:36:45 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Nov 24 11:36:55 2008 Subject: [Histonet] Iron stains In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E05F@lmhsmail.lmhealth.org> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E05F@lmhsmail.lmhealth.org> Message-ID: <5D64396A0D4A5346BEBC759022AAEAA5169CD7@ITSSSXM01V6.one.ads.che.org> I would always fix first before decal. The acid is stripping the iron. After discontinuing B5 fixative, we use B-Plus (zinc), but some are very happy with 10% NBF. The problem with purchased controls is that they are not treated the same as your material, but at least you know your reagents are good! My 2 cents... J:>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Monday, November 24, 2008 12:07 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Iron stains We may be having a problem with our iron stains. I say that we 'may' be having a problem because our positive control always works. For bone marrows, we stain the core, aspirate, a smear, and a purchased positive control. The stain is all over the place. We may have a loaded smear with a negative core and aspirate. We may have a positive aspirate with a negative core and smear. We may have all negatives even though clinically, there should be iron. Our control always works. The pathologists do not trust the stain and really do not believe the results. I have stood by the stain due to the positive control but I find it increasingly difficult. I would appreciate any thoughts. Our procedures are outlined below... The aspirate is allowed to clot before placing it into 10%NBF. The core is placed briefly into DecalStat (until it floats) then placed into 10%NBF. Both are then processed with our regular tissues. The spend anywhere from 4 to 10 hours in formalin. The following day, they are cut and along with one of the purchased controls, run down to water on the stainer. (8 mins onboard oven, americlear, alcohols, water) The smear is placed into 95% alcohol then rinsed in distilled. All slides are then stained using Perl's Method for iron pigment. We have already explored the way the specimens are collected during the BM procedure, and decal solution. All reagents are good. I'm wondering about the formalin fixation. I know that threre are other probably better fixatives. Does anybody use another fixative prior to formalin? For those using formalin only, do you limit the fixation time in any way? I really would appreciate any comments. Such a common and simple stain. We should be able to trust it. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From lpwenk <@t> sbcglobal.net Mon Nov 24 11:47:24 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Nov 24 11:47:29 2008 Subject: [Histonet] Dissolve plastic Message-ID: <001d01c94e5c$b63a94a0$0202a8c0@HPPav2> I Need Histonetters help. We have an eye with a plastic part for the lens, which is a type of telescope, I've been told. According to the new pathologist, we need to dissolve out this plastic lens, then he can gross the eye and we can process it into paraffin. We've never had to dissolve plastic before. The eye is in 10% NBF right now. I'm guess maybe something like chloroform? But what percent? For how long? Before or after grossing? Has anyone done this before, that can send a procedure to me? Thanks. *************************************** Peggy A. Wenk, HTL(ASCP)SLS Program Director, Schools of Histotechnology Supervisor, Mortuary Services Laboratory Safety Officer, Disaster Preparedness and Quality Assurance Coordinator Department of Anatomic Pathology, 100RO William Beaumont Hospital 3601 W. 13 Mile Road Royal Oak, MI 48073-6769 Work 248/898-9079 Fax 248/898-9054 E-mail pwenk@beaumont.edu Web Page: http://www.beaumont.edu/alliedhealth From rjbuesa <@t> yahoo.com Mon Nov 24 12:10:40 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 24 12:10:45 2008 Subject: [Histonet] Dissolve plastic In-Reply-To: <001d01c94e5c$b63a94a0$0202a8c0@HPPav2> Message-ID: <497669.23889.qm@web65711.mail.ac4.yahoo.com> Try pure acetone that will also start the dehydration of the eye. Ren? J. --- On Mon, 11/24/08, Lee & Peggy Wenk wrote: From: Lee & Peggy Wenk Subject: [Histonet] Dissolve plastic To: histonet@lists.utsouthwestern.edu Date: Monday, November 24, 2008, 12:47 PM I Need Histonetters help. We have an eye with a plastic part for the lens, which is a type of telescope, I've been told. According to the new pathologist, we need to dissolve out this plastic lens, then he can gross the eye and we can process it into paraffin. We've never had to dissolve plastic before. The eye is in 10% NBF right now. I'm guess maybe something like chloroform? But what percent? For how long? Before or after grossing? Has anyone done this before, that can send a procedure to me? Thanks. *************************************** Peggy A. Wenk, HTL(ASCP)SLS Program Director, Schools of Histotechnology Supervisor, Mortuary Services Laboratory Safety Officer, Disaster Preparedness and Quality Assurance Coordinator Department of Anatomic Pathology, 100RO William Beaumont Hospital 3601 W. 13 Mile Road Royal Oak, MI 48073-6769 Work 248/898-9079 Fax 248/898-9054 E-mail pwenk@beaumont.edu Web Page: http://www.beaumont.edu/alliedhealth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kalschev <@t> svm.vetmed.wisc.edu Mon Nov 24 12:35:17 2008 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Mon Nov 24 12:35:48 2008 Subject: [Histonet] Peggy - eye/plastic lens Message-ID: <001301c94e63$65cd4510$c5d76880@vetmed.wisc.edu> Hi Peggy: It is possible that acetone ( swab on, or soak) will dissolve or loosen the lens without cellular damage. I have not done this and will be interested to see what other suggestions are made. Vicki From RSRICHMOND <@t> aol.com Mon Nov 24 12:42:48 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Mon Nov 24 12:42:53 2008 Subject: [Histonet] Re: Floaters Message-ID: Teresa Hallada in Michigan asks: >>Would anyone care to share their policy on floaters? Also, does anyone know if there is a CAP policy [on] floaters?<< At Gastonia we sent 'em on to the state ME's office - they had an adequately ventilated autopsy suite.... But seriously, folks, I've never heard of any regulatory agency having a policy on floaters - extraneous tissue on a slide that's floated in from some other case. What I was taught in residency - sometime in the late Ordovician period, I think it was - not to mention them in a report, but write "floater" on the slide before filing it. I've never heard anybody observe this, but there are two kinds of floaters: individual tissue sections (the responsibility of the microtomist) and whole chunks of tissue (the responsibility of whoever grossed the specimen). Since the trouble-shooting for these two problems is entirely different, you first have to determine which kind of floater you've got. Obviously if it's a whole chunk of tissue, you'll get repeated sections of it. If it's whole chunks of tissue, then the grosser isn't cleaning instruments adequately between cases. If I ran the zoo, I'd have several sets of instruments, throw them in a pot as I used them, and wash them when I ran out of instruments, but the Hospital Administrator's Official Handy-Dandy Book on How to Make Life Hard for the Anatomic Pathology Service specifies that there be only one set of grossing instruments in the lab at a time. (I've never actually seen this book, but I'm absolutely sure it exists.) Bob Richmond Samurai Pathologist Knoxville TN From jcline <@t> wchsys.org Mon Nov 24 12:42:58 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Mon Nov 24 12:43:07 2008 Subject: [Histonet] Leica slide writer - problems?? In-Reply-To: <90B6684A9D6DAF468F7A5DC148754E1DC21C00@ALPW31.f2.enterprise> Message-ID: This problem was solved a few months back, by switching to a different slide. Joyce Cline -----Original Message----- From: Stephen.Eyres@sanofi-aventis.com [mailto:Stephen.Eyres@sanofi-aventis.com] Sent: Monday, November 24, 2008 10:38 AM To: jcline@wchsys.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica slide writer - problems?? Hi Joyce, Was this a recent problem, and has it been resolved? Thanks Steve -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Thursday, November 20, 2008 6:20 PM To: Histonet Subject: RE: [Histonet] Leica slide writer - problems?? We have had the same problems, we discovered the slide surface on some of the snowcoat slides changed to a slick surface instead of a rough surface. We have switched to a pale gray slide that still has the rough surface and we have not had any printing wipe off. Joyce Cline, H.T. (ASCP) Technical Specialist Hagerstown Medical Lab. 301-665-4980 fax 301-665-4941 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara.Lees@sanofi-aventis.com Sent: Thursday, November 20, 2008 7:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica slide writer - problems?? Hi, Has anyone else been having problems with there Lieca slide writer. Our problems mainly involve the ink writing rubbing off before and after staining!!, we use Surgipath snowcoated slides, what does everyone else use as they have hinted it could be our slides. Any help would be fantastic. Thanks Loads Sara _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From contact <@t> excaliburpathology.com Mon Nov 24 13:41:26 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Nov 24 13:41:30 2008 Subject: [Histonet] Peggy - eye/plastic lens Message-ID: <534075.12047.qm@web1105.biz.mail.sk1.yahoo.com> I have processed many, many eyes with artificial lenses. The older lenses dissolved out in chloroform, however the newer plastics in use do not. I disect the colottes off either side of the iris and optic nerve and process the central section, IOL and all. When disecting, the haptics, which hold the IOL in place, will cut easily.?If there is no fibrous tissue or lens capsule surrounding the IOL, it may dislodge during processing. But I have sectioned eyes with the IOL in place. FYI, NBF will cause retinal detachment. Paula ________________________________ From: Vicki Kalscheur To: Histonet Discussion Sent: Monday, November 24, 2008 12:35:17 PM Subject: [Histonet] Peggy - eye/plastic lens Hi Peggy:? It is possible that acetone ( swab on, or soak) will dissolve or loosen the lens without cellular damage. I have not done this and will be interested to see what other suggestions are made.? Vicki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kenneth.a.troutman <@t> Vanderbilt.Edu Mon Nov 24 13:45:17 2008 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Mon Nov 24 13:45:35 2008 Subject: [Histonet] ISH question Message-ID: <37DEF9AF72994947AF693956A59B9B660128001C@mailbe03.mc.vanderbilt.edu> Great question... I'll have to try this. My immediate thought is "does the hematoxylin interfere?" I'll run a slide this week and let you know... Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN Message: 23 Date: Sat, 22 Nov 2008 15:12:27 +0100 From: "Gudrun Lang" Subject: [Histonet] ISH question To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi listmembers, In our lab the unstained IHC-slides are thrown away. Now one slide of each case should be stored in an adequate manner to perform an insitu hybridization if required in the future. I want to store it unstained but deparaffinized and coverslipped. So that it can be filed with the other HE-slides and easily found again. We don't want to store them airsealed in the freezer, and IHC is no issue. Has anybody performed an in situ hybridization on previously coverslipped or even stained tissue? I think, that the DNA would be well preserved. What could be a cause of DNA-degradation in this conservation-manner? Thanks for sharing your experiences. Gudrun Lang Histolab, Akh Linz, Austria From Jacqueline.Farnsworth <@t> cls.ab.ca Mon Nov 24 14:30:29 2008 From: Jacqueline.Farnsworth <@t> cls.ab.ca (Jacqueline Farnsworth) Date: Mon Nov 24 14:30:34 2008 Subject: [Histonet] bone saws? Message-ID: Greetings I am wondering if anyone can share how they are cutting femoral heads, extremities for tumour, etc. We currently use a 20 yr + meat saw (band saw), but would like to find something safer/newer. We freeze all our bones (pre-fixation and pre-decalcification) prior to cutting. Vendors welcome to reply. Thanks in advance Jacquie Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From Jacqueline.Farnsworth <@t> cls.ab.ca Mon Nov 24 14:49:35 2008 From: Jacqueline.Farnsworth <@t> cls.ab.ca (Jacqueline Farnsworth) Date: Mon Nov 24 14:49:43 2008 Subject: [Histonet] Tissue Tek cryostat Message-ID: We have a few Tissue Tek cryostats still around ..(a lot of TLC to over the years for these)! We still love our old stand-bys. We are wondering if anyone would have a disposable blade holder that may be collecting dust ? We'd like to purchase one to make another compete functioning cryostat. Thanks again, Jacquie Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From settembr <@t> umdnj.edu Mon Nov 24 15:06:40 2008 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Mon Nov 24 15:12:34 2008 Subject: [Histonet] immuno set-up Message-ID: I agree with Mr. Brooks on the Shandon Sequenza - fantastic for low volume. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Amos Brooks 11/21/08 4:58 PM >>> Hi, For small volumes like that, check with Thermo. They have various sizes of IHC stainers that have different slide capacities. Alternatively you could check out the Shandon Sequenza. I could find more info if you are interested. (BTW: I have no Thermo ties.) Amos Brooks Message: 19 Date: Thu, 20 Nov 2008 20:21:21 +0000 From: zodiac29@comcast.net Subject: [Histonet] immuno set-up To: histonet@lists.utsouthwestern.edu Message-ID: < 112020082021.29733.4925C6C10003F44E000074252214756402C7CD0C0E070B0196@comcast.net > Content-Type: text/plain Hello all, I work at a private lab with one pathologist, and one histotech (me). We do about 5,000 cases a year. We are intrested in doing our immuno's in house (right now we send them out), and wanted to know your opinion on the type of equipment that would be best for this situation. Right now we send about 5 to 10 cases a week out for immuno's. All responses are very much appreciated Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Mon Nov 24 15:17:30 2008 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Mon Nov 24 15:17:36 2008 Subject: [Histonet] Error question Message-ID: <29BE166A2CF48D459853F8EC57CD37E801A82CBC@EXCHANGECLUSTER.yumaregional.local> Was wanting to know how people handle accession errors within the pathology information system,, to elaborate on this, is when a person receives in the specimen with a requisition and they have to do data entry into the system. What is allowable?, what is the percent error rate?, how are people dealing with errors? How are you dealing with personnel?? This is becoming an issue and I wanted to survey people out there how they are handling this issue,? Any help would greatly be appreciated. Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 * Office: (928) 336-1743 * Fax: (928) 336-7319 * Email: jellin@yumaregional.org This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. From dellav <@t> musc.edu Mon Nov 24 16:37:29 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Mon Nov 24 16:37:35 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? Message-ID: I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 From JWeems <@t> sjha.org Mon Nov 24 16:40:38 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Nov 24 16:40:45 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? In-Reply-To: References: Message-ID: <5D64396A0D4A5346BEBC759022AAEAA5169D84@ITSSSXM01V6.one.ads.che.org> Could you give it to her in a plastic bag, with a container, and tell her to store it in rubbing alcohol? j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone and fax numbers 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Monday, November 24, 2008 5:37 PM To: histonet Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From saby_joseph_a <@t> yahoo.com Mon Nov 24 17:10:45 2008 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Mon Nov 24 17:10:48 2008 Subject: [Histonet] Dissolve plastic References: <001d01c94e5c$b63a94a0$0202a8c0@HPPav2> Message-ID: <189959.36275.qm@web33801.mail.mud.yahoo.com> Lee and Peggy- We section these eyes all the time with the plastic in place.? I would certainly suggest a gluaraldehyde fixative.? Straight NBF is a very poor fixative for the many tissues found in eyes, especially the retina.? If you contact me directly, we can talk off line.? Eyes have held a special fascination with me for many years. Joe Saby NAMSA ________________________________ From: Lee & Peggy Wenk To: histonet@lists.utsouthwestern.edu Sent: Monday, November 24, 2008 12:47:24 PM Subject: [Histonet] Dissolve plastic I Need Histonetters help. We have an eye with a plastic part for the lens, which is a type of telescope, I've been told. According to the new pathologist, we need to dissolve out this plastic lens, then he can gross the eye and we can process it into paraffin. We've never had to dissolve plastic before. The eye is in 10% NBF right now. I'm guess maybe something like chloroform? But what percent? For how long? Before or after grossing? Has anyone done this before, that can send a procedure to me? Thanks. *************************************** Peggy A. Wenk, HTL(ASCP)SLS Program Director,? Schools of Histotechnology Supervisor, Mortuary Services Laboratory Safety Officer, Disaster Preparedness and Quality Assurance Coordinator Department of Anatomic Pathology, 100RO William Beaumont Hospital 3601 W. 13 Mile Road Royal Oak, MI 48073-6769 Work? 248/898-9079 Fax? ? ? 248/898-9054 E-mail? pwenk@beaumont.edu Web Page: http://www.beaumont.edu/alliedhealth _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Nov 24 17:13:25 2008 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Nov 24 17:13:30 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? In-Reply-To: <5D64396A0D4A5346BEBC759022AAEAA5169D84@ITSSSXM01V6.one.ads.che.org> References: <5D64396A0D4A5346BEBC759022AAEAA5169D84@ITSSSXM01V6.one.ads.che.org> Message-ID: You know, I really think that the vinegar is a great idea! I can't think of any reason off-hand why it would be hazardous, flammable, toxic, or bring on any liability. Even the isopropyl can be flammable, and if you told her to use it, you might be somewhat at fault ( though I know a bit of a stretch). In any case, I think that I'll try to remember that in case I encounter any similar requests... Thanks Joelle> Date: Mon, 24 Nov 2008 17:40:38 -0500> From: JWeems@sjha.org> To: dellav@musc.edu; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ?> CC: > > Could you give it to her in a plastic bag, with a container, and tell> her to store it in rubbing alcohol? j> > Joyce Weems> Pathology Manager> Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE> Atlanta, GA 30342> Please note new phone and fax numbers> 678-843-7376 - Phone> 678-843-7831 - Fax> > > > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della> Speranza, Vinnie> Sent: Monday, November 24, 2008 5:37 PM> To: histonet> Subject: [Histonet] as Thanksgiving approaches, what do you think about> a gall bladder in vinegar ?> > I have a patient requesting her gall bladder be returned to her for> religious reasons.> The premise I've been given is so that, upon death, the patient may be> stored with her body parts.> > My facility has concerns about providing it to her in formalin (for> obvious reasons) or alcohol. The patient admits this is a family> practice with momma's appendix already being stored in the attic.> > It can get a bit toasty warm here in the South so attic storage of a> specimen in alcohol may not be prudent and I can't be absolutely certain> it wouldn't burn the house down, another potential liability for my> institution.> > I'm tempted to give it to her in food grade vinegar, to avoid the> potential liabilities from using anything that could be considered> hazardous.> Assuming that returning her gall bladder is a given, what do you think> of using vinegar for this purpose?> > > > Vinnie Della Speranza> > Manager for Anatomic Pathology Services> > Medical University of South Carolina> > 165 Ashley Avenue Suite 309> > Charleston, South Carolina 29425> > Tel: (843) 792-6353> > Fax: (843) 792-8974> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> Confidentiality Notice:> This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use,> disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Access your email online and on the go with Windows Live Hotmail. http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_access_112008 From amosbrooks <@t> gmail.com Mon Nov 24 18:40:33 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Nov 24 18:40:37 2008 Subject: [Histonet] Iron stains Message-ID: <582736990811241640v42256071u11001b4a63f226@mail.gmail.com> Tom, I'm betting your control and test slides are right, but there is a fairly quick & dirty method of making sure. Don't process ALL the aspirates with the rest of the case. Make some smears of them directly. Do try to pick up some of the smaller spicule material (centrifuge if necessary) and when you place it on the slides crush them up with edge of the smearing slide. Make the smears and let them air dry (don't worry, you're looking for iron here not necessarily morphology). Once they are dried place them gently in methanol (I'm not really sure why we did this since it was air dried already) and re hydrate to distilled H2O. Then run your usual Iron stain with a control of course. This should confirm the results of the processed material. If this is positive and your processed material is not THEN you can panic. This should at least alleviate the Dr's concerns. If/when you find a nice positive one (hemochromatosis ?sp?) take about 20-50 slides and sit down and make a bunch of smears to store in a slide box for future controls (the Iron isn't going anywhere). Best of luck, Amos Message: 12 Date: Mon, 24 Nov 2008 12:06:59 -0500 From: "Tom McNemar" Subject: [Histonet] Iron stains To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E05F@lmhsmail.lmhealth.org> Content-Type: text/plain; charset="iso-8859-1" We may be having a problem with our iron stains. I say that we 'may' be having a problem because our positive control always works. For bone marrows, we stain the core, aspirate, a smear, and a purchased positive control. The stain is all over the place. We may have a loaded smear with a negative core and aspirate. We may have a positive aspirate with a negative core and smear. We may have all negatives even though clinically, there should be iron. Our control always works. The pathologists do not trust the stain and really do not believe the results. I have stood by the stain due to the positive control but I find it increasingly difficult. I would appreciate any thoughts. Our procedures are outlined below... The aspirate is allowed to clot before placing it into 10%NBF. The core is placed briefly into DecalStat (until it floats) then placed into 10%NBF. Both are then processed with our regular tissues. The spend anywhere from 4 to 10 hours in formalin. The following day, they are cut and along with one of the purchased controls, run down to water on the stainer. (8 mins onboard oven, americlear, alcohols, water) The smear is placed into 95% alcohol then rinsed in distilled. All slides are then stained using Perl's Method for iron pigment. We have already explored the way the specimens are collected during the BM procedure, and decal solution. All reagents are good. I'm wondering about the formalin fixation. I know that threre are other probably better fixatives. Does anybody use another fixative prior to formalin? For those using formalin only, do you limit the fixation time in any way? I really would appreciate any comments. Such a common and simple stain. We should be able to trust it. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org From AnthonyH <@t> chw.edu.au Mon Nov 24 19:29:32 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Nov 24 19:29:48 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? In-Reply-To: Message-ID: Vinnie, You could use paraffin oil (also known as mineral oil). It is used successfully in museum techniques for the preservation of formalin fixed specimens. Rinse the formalin fixed specimen in water, place in ethanol (which will also bring back the colour), blot lightly and place in the oil. The oil has the added advantage that bile will not tend to leach out of the specimen. It also will not evaporate. See: Henwood (2002)"Color preservation in pathology museum specimens" published in Biotech Histochem 2002 Jul; 77(4): 230. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, 25 November 2008 9:37 AM To: histonet Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Barry.R.Rittman <@t> uth.tmc.edu Mon Nov 24 22:07:14 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon Nov 24 22:08:55 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? References: Message-ID: Vinnie You can also use glycerin. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tony Henwood Sent: Mon 11/24/2008 7:29 PM To: Della Speranza, Vinnie; histonet Subject: RE: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? Vinnie, You could use paraffin oil (also known as mineral oil). It is used successfully in museum techniques for the preservation of formalin fixed specimens. Rinse the formalin fixed specimen in water, place in ethanol (which will also bring back the colour), blot lightly and place in the oil. The oil has the added advantage that bile will not tend to leach out of the specimen. It also will not evaporate. See: Henwood (2002)"Color preservation in pathology museum specimens" published in Biotech Histochem 2002 Jul; 77(4): 230. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, 25 November 2008 9:37 AM To: histonet Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonet.nospam <@t> vneubert.com Tue Nov 25 01:48:52 2008 From: histonet.nospam <@t> vneubert.com (V. Neubert) Date: Tue Nov 25 01:48:56 2008 Subject: [Histonet] Warthin-Starry // "Best before" dates Message-ID: <39eee79085e2ff5f22bd5b83b59b0379@vneubert.com> Hello! I use the Warthin-Starry technique to stain spirochaetes in FFPE sections. I don't know how long I can keep the AgNO_3 solutions and the hydrochinone solution whitout getting them to "expire". Is there anyone who already had to deal with that question? Regards, Valentin From louise.renton <@t> gmail.com Tue Nov 25 02:24:13 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Nov 25 02:24:25 2008 Subject: [Histonet] osteoid Message-ID: Thnaks for all the replies I got on this subject - they have clarified my thoughts somewhat -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From lpwenk <@t> sbcglobal.net Tue Nov 25 04:34:11 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Nov 25 04:34:17 2008 Subject: [Histonet] Warthin-Starry // "Best before" dates In-Reply-To: <39eee79085e2ff5f22bd5b83b59b0379@vneubert.com> Message-ID: <000301c94ee9$5b7b6610$0202a8c0@HPPav2> We make up the hydroquinone reducing solution just before use, but found for the Steiner, since it makes up 60 mL, that if we use half and store the other half in the refrig, we can use it the next day. After that, it doesn't work very well. As for silver solutions, if you are talking about a certain percent aqueous silver nitrate (say 1% aq. Silver nitrate), we found aq. Silver nitrate is good in the refrig for about 6 months, so we only make up enough to last 2-3 months, to be on the safe side. If you are talking about the silver made up in a buffer solution, then I don't know how long it is good for. If you don't do W-S very often, it just might be best if you make both of these solutions up when the stain is requested. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of V. Neubert Sent: Tuesday, November 25, 2008 2:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Warthin-Starry // "Best before" dates Hello! I use the Warthin-Starry technique to stain spirochaetes in FFPE sections. I don't know how long I can keep the AgNO_3 solutions and the hydrochinone solution whitout getting them to "expire". Is there anyone who already had to deal with that question? Regards, Valentin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Tue Nov 25 08:02:51 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Nov 25 08:03:03 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? In-Reply-To: Message-ID: <000001c94f06$84f58210$d00f7ca5@lurie.northwestern.edu> Vinnie, We had an issue with this concerning tonsils (the kids wanted them). We rinsed out the excess formalin after the final sign out by a pathologist (2 weeks), patted it dry and gave the sample to them in a clean container. This way it was fixed but they did not have the issue of formalin. I'm guessing that 2 weeks fixation should be sufficient for everything to be fixed. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Monday, November 24, 2008 4:37 PM To: histonet Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Weber2 <@t> va.gov Tue Nov 25 08:07:23 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Tue Nov 25 08:07:33 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? In-Reply-To: References: Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76E95@VHAV10MSGA1.v10.med.va.gov> Does she already have a funeral home picked out? Perhaps she can ask the funeral home to "store" it for her, and then release it only to a funeral home. I would consult my legal department to see what they feel is appropriate, that way you are dotting all your t's and crossing your eyes >.< as well! Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Monday, November 24, 2008 5:37 PM To: histonet Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Tue Nov 25 08:13:30 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Nov 25 08:13:35 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A39C6@IS-E2K3.grhs.net> I was is this "pickle" once, and we washed the specimen and then just gave it to the patient clean and dry in a clean container. It was then up to the patient to figure out what to do with the specimen. I believe they had it frozen at the funeral home. Sorry Vinnie, could not pass up the pickle joke here. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Monday, November 24, 2008 4:37 PM To: histonet Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Nov 25 08:27:28 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 25 08:27:31 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? In-Reply-To: Message-ID: <740052.79950.qm@web65703.mail.ac4.yahoo.com> I think it is a good idea of a pickled gall bladder. Do not add onions please! Ren? J. --- On Mon, 11/24/08, Della Speranza, Vinnie wrote: From: Della Speranza, Vinnie Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? To: "histonet" Date: Monday, November 24, 2008, 5:37 PM I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCollins <@t> palmbeachpath.com Tue Nov 25 08:37:38 2008 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Tue Nov 25 08:37:43 2008 Subject: [Histonet] Re: As Thanksgiving Approaches Message-ID: <05CAE76AB5D5ED409864C6DD86F13349024DB5E7B8@pbpsflexch02.pbp.local> One thing to keep in mind about Vinegar, however, is that fungus will still grow in it after a while. Kind of gross! Judy Collins From rfields <@t> gidocs.net Tue Nov 25 08:40:06 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Tue Nov 25 08:42:00 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? Message-ID: <07732CE52EC3174AB891DE1C62DB4D8F43ED65@GIEXCHANGE.gidocs.net> I would have to agree, the vinegar sounds like a good solution. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Monday, November 24, 2008 4:37 PM To: histonet Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Tue Nov 25 08:51:28 2008 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Nov 25 08:54:23 2008 Subject: [Histonet] RE: Re: As Thanksgiving Approaches In-Reply-To: <05CAE76AB5D5ED409864C6DD86F13349024DB5E7B8@pbpsflexch02.pbp.local> References: <05CAE76AB5D5ED409864C6DD86F13349024DB5E7B8@pbpsflexch02.pbp.local> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D2F0F96E8@LRGHEXVS1.practice.lrgh.org> Once specimens arrive at our lab, they are ours. We do not give anything back to the patient. No legal liability that way. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judy Collins [JCollins@palmbeachpath.com] Sent: Tuesday, November 25, 2008 9:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: As Thanksgiving Approaches One thing to keep in mind about Vinegar, however, is that fungus will still grow in it after a while. Kind of gross! Judy Collins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From ree3 <@t> leicester.ac.uk Tue Nov 25 08:57:59 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Nov 25 08:58:28 2008 Subject: [Histonet] RE: Re: As Thanksgiving Approaches In-Reply-To: <05CAE76AB5D5ED409864C6DD86F13349024DB5E7B8@pbpsflexch02.pbp.local> References: <05CAE76AB5D5ED409864C6DD86F13349024DB5E7B8@pbpsflexch02.pbp.local> Message-ID: <7722595275A4DD4FA225B92CDBF174A1744E1B4645@EXC-MBX3.cfs.le.ac.uk> and nematodes, surprisingly known in the trade as vinegar worms; for people in the U.K., when you visit the chippie and pick up the vinegar bottle, the cloudiness you observe if you give it a good shake is/are nematodes, not to worry tho', consider it extra protein, something for nothing, not to be sniffed at in these creditcrunchy times!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judy Collins Sent: 25 November 2008 14:38 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: As Thanksgiving Approaches One thing to keep in mind about Vinegar, however, is that fungus will still grow in it after a while. Kind of gross! Judy Collins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Vacca <@t> HCAhealthcare.com Tue Nov 25 08:59:37 2008 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Tue Nov 25 08:59:42 2008 Subject: [Histonet] RE: Re: As Thanksgiving Approaches In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638631D2F0F96E8@LRGHEXVS1.practice.lrgh.org> References: <05CAE76AB5D5ED409864C6DD86F13349024DB5E7B8@pbpsflexch02.pbp.local> <38667E7FB77ECD4E91BFAEB8D98638631D2F0F96E8@LRGHEXVS1.practice.lrgh.org> Message-ID: <938D716CD445614ABBB817517557B6F43A464493@NADCWPMSGCMS09.hca.corpad.net> We will only release items to the funeral home, Find out if they already have arrangements with one and release it to them. Otherwise it won't get released. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Tuesday, November 25, 2008 9:51 AM To: Judy Collins; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Re: As Thanksgiving Approaches Once specimens arrive at our lab, they are ours. We do not give anything back to the patient. No legal liability that way. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judy Collins [JCollins@palmbeachpath.com] Sent: Tuesday, November 25, 2008 9:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: As Thanksgiving Approaches One thing to keep in mind about Vinegar, however, is that fungus will still grow in it after a while. Kind of gross! Judy Collins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Tue Nov 25 09:17:10 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Nov 25 09:18:00 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? In-Reply-To: <16C83872A53F4346AA9C3A18E3A3AAB903F76E95@VHAV10MSGA1.v10.med.va.gov> References: <16C83872A53F4346AA9C3A18E3A3AAB903F76E95@VHAV10MSGA1.v10.med.va.gov> Message-ID: I'm overwhelmed at the huge response. Thank you all. The patient was instructed to make arrangements with a funeral home. The patient is 29 yrs old and since the funeral home might be taking on the responsibility of storing for several decades, she's been unsuccessful in identifying one willing to assist her. I don't have any way to know what the mom's appendix is in. it's quite possible she obtained it before the regs on formaldehyde became so restrictive (mid- '80's I believe). The vinegar was thought to be a solution that would not grow organisms. The gall bladder has been cut down and now consists of a few very thin strips so I was concerned that allowing the tissue to air dry may appear (to the patient) that the specimen had been compromised. Have a great holiday everyone, and thank you again. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: Weber, Susan (VHACLE) [mailto:Susan.Weber2@va.gov] Sent: Tuesday, November 25, 2008 9:07 AM To: Della Speranza, Vinnie; histonet Subject: RE: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? Does she already have a funeral home picked out? Perhaps she can ask the funeral home to "store" it for her, and then release it only to a funeral home. I would consult my legal department to see what they feel is appropriate, that way you are dotting all your t's and crossing your eyes >.< as well! Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Monday, November 24, 2008 5:37 PM To: histonet Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Tue Nov 25 09:17:58 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Nov 25 09:18:04 2008 Subject: [Histonet] California State Regulations Message-ID: <57BE698966D5C54EAE8612E8941D76830441047B@EXCHANGE3.huntingtonhospital.com> I'm looking for information regarding hospital labs in California. I need to know if Calif state regulations are more strict than CAP regarding record retention. CAP says we need to keep logs for 2 years. I'm getting varying info on state regs - 3 years, 6 years, 10 years. I am wondering how long other labs are keeping accession logs, IHC logs, special stain/IHC requests, etc. Thanks in advance. Laurie Colbert From dellav <@t> musc.edu Tue Nov 25 09:29:00 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Nov 25 09:29:13 2008 Subject: [Histonet] RE: Re: As Thanksgiving Approaches In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638631D2F0F96E8@LRGHEXVS1.practice.lrgh.org> References: <05CAE76AB5D5ED409864C6DD86F13349024DB5E7B8@pbpsflexch02.pbp.local> <38667E7FB77ECD4E91BFAEB8D98638631D2F0F96E8@LRGHEXVS1.practice.lrgh.org> Message-ID: Tom, I'd normally take this approach but if push came to shove I don't believe it would hold up in court. That may depend on the language in your surgical consent signed by the patient but that aside, the cost of responding to a patient's legal action would be much greater than the small effort it takes to render the specimen safer to turn over. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Tuesday, November 25, 2008 9:51 AM To: Judy Collins; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Re: As Thanksgiving Approaches Once specimens arrive at our lab, they are ours. We do not give anything back to the patient. No legal liability that way. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judy Collins [JCollins@palmbeachpath.com] Sent: Tuesday, November 25, 2008 9:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: As Thanksgiving Approaches One thing to keep in mind about Vinegar, however, is that fungus will still grow in it after a while. Kind of gross! Judy Collins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Nov 25 09:40:53 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Nov 25 09:39:06 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? In-Reply-To: References: <16C83872A53F4346AA9C3A18E3A3AAB903F76E95@VHAV10MSGA1.v10.med.va.gov> Message-ID: <5A2BD13465E061429D6455C8D6B40E3907C05DF202@IBMB7Exchange.digestivespecialists.com> Would processing the entire specimen and embedding it in paraffin be a solution? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, November 25, 2008 10:17 AM To: 'Weber, Susan (VHACLE)'; histonet Subject: RE: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I'm overwhelmed at the huge response. Thank you all. The patient was instructed to make arrangements with a funeral home. The patient is 29 yrs old and since the funeral home might be taking on the responsibility of storing for several decades, she's been unsuccessful in identifying one willing to assist her. I don't have any way to know what the mom's appendix is in. it's quite possible she obtained it before the regs on formaldehyde became so restrictive (mid- '80's I believe). The vinegar was thought to be a solution that would not grow organisms. The gall bladder has been cut down and now consists of a few very thin strips so I was concerned that allowing the tissue to air dry may appear (to the patient) that the specimen had been compromised. Have a great holiday everyone, and thank you again. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: Weber, Susan (VHACLE) [mailto:Susan.Weber2@va.gov] Sent: Tuesday, November 25, 2008 9:07 AM To: Della Speranza, Vinnie; histonet Subject: RE: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? Does she already have a funeral home picked out? Perhaps she can ask the funeral home to "store" it for her, and then release it only to a funeral home. I would consult my legal department to see what they feel is appropriate, that way you are dotting all your t's and crossing your eyes >.< as well! Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Monday, November 24, 2008 5:37 PM To: histonet Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Tue Nov 25 09:50:22 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Nov 25 09:50:27 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think abouta gall bladder in vinegar ? In-Reply-To: <5A2BD13465E061429D6455C8D6B40E3907C05DF202@IBMB7Exchange.digestivespecialists.com> References: <16C83872A53F4346AA9C3A18E3A3AAB903F76E95@VHAV10MSGA1.v10.med.va.gov> <5A2BD13465E061429D6455C8D6B40E3907C05DF202@IBMB7Exchange.digestivespecialists.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82F19@EMAIL.archildrens.org> I like this idea best. You could just make one big block. Of course, you will have to tell them NOT to store it in the attic. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, November 25, 2008 9:41 AM To: 'Della Speranza, Vinnie'; histonet Subject: RE: [Histonet] as Thanksgiving approaches, what do you think abouta gall bladder in vinegar ? Would processing the entire specimen and embedding it in paraffin be a solution? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, November 25, 2008 10:17 AM To: 'Weber, Susan (VHACLE)'; histonet Subject: RE: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I'm overwhelmed at the huge response. Thank you all. The patient was instructed to make arrangements with a funeral home. The patient is 29 yrs old and since the funeral home might be taking on the responsibility of storing for several decades, she's been unsuccessful in identifying one willing to assist her. I don't have any way to know what the mom's appendix is in. it's quite possible she obtained it before the regs on formaldehyde became so restrictive (mid- '80's I believe). The vinegar was thought to be a solution that would not grow organisms. The gall bladder has been cut down and now consists of a few very thin strips so I was concerned that allowing the tissue to air dry may appear (to the patient) that the specimen had been compromised. Have a great holiday everyone, and thank you again. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: Weber, Susan (VHACLE) [mailto:Susan.Weber2@va.gov] Sent: Tuesday, November 25, 2008 9:07 AM To: Della Speranza, Vinnie; histonet Subject: RE: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? Does she already have a funeral home picked out? Perhaps she can ask the funeral home to "store" it for her, and then release it only to a funeral home. I would consult my legal department to see what they feel is appropriate, that way you are dotting all your t's and crossing your eyes >.< as well! Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Monday, November 24, 2008 5:37 PM To: histonet Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From tpodawiltz <@t> lrgh.org Tue Nov 25 09:55:28 2008 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Nov 25 09:59:32 2008 Subject: [Histonet] RE: Re: As Thanksgiving Approaches In-Reply-To: References: <05CAE76AB5D5ED409864C6DD86F13349024DB5E7B8@pbpsflexch02.pbp.local> <38667E7FB77ECD4E91BFAEB8D98638631D2F0F96E8@LRGHEXVS1.practice.lrgh.org>, Message-ID: <38667E7FB77ECD4E91BFAEB8D98638631D2F0F96EB@LRGHEXVS1.practice.lrgh.org> Vinnie, its all in how the Physicians explain it to the patients and state requirements, it has never been an issue, yet. But you know how that goes, haven't meant a hospital lawyer that has not rolled over when push came to shove. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: Della Speranza, Vinnie [dellav@musc.edu] Sent: Tuesday, November 25, 2008 10:29 AM To: Podawiltz, Thomas; Judy Collins; Histonet@lists.utsouthwestern.edu Subject: RE: Re: As Thanksgiving Approaches Tom, I'd normally take this approach but if push came to shove I don't believe it would hold up in court. That may depend on the language in your surgical consent signed by the patient but that aside, the cost of responding to a patient's legal action would be much greater than the small effort it takes to render the specimen safer to turn over. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Tuesday, November 25, 2008 9:51 AM To: Judy Collins; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Re: As Thanksgiving Approaches Once specimens arrive at our lab, they are ours. We do not give anything back to the patient. No legal liability that way. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judy Collins [JCollins@palmbeachpath.com] Sent: Tuesday, November 25, 2008 9:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: As Thanksgiving Approaches One thing to keep in mind about Vinegar, however, is that fungus will still grow in it after a while. Kind of gross! Judy Collins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From JMacDonald <@t> mtsac.edu Tue Nov 25 10:03:13 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Nov 25 10:03:13 2008 Subject: [Histonet] RE: Re: As Thanksgiving Approaches In-Reply-To: Message-ID: Vinnie, Check the forms that are filled out by the patient upon admission. Many hospitals have that covered by the paperwork that the patient signs. It covers specimens and such. Jennifer "Della Speranza, Vinnie" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/25/2008 07:30 AM To "'Podawiltz, Thomas'" , Judy Collins , "Histonet@lists.utsouthwestern.edu" cc Subject [Histonet] RE: Re: As Thanksgiving Approaches Tom, I'd normally take this approach but if push came to shove I don't believe it would hold up in court. That may depend on the language in your surgical consent signed by the patient but that aside, the cost of responding to a patient's legal action would be much greater than the small effort it takes to render the specimen safer to turn over. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Tuesday, November 25, 2008 9:51 AM To: Judy Collins; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Re: As Thanksgiving Approaches Once specimens arrive at our lab, they are ours. We do not give anything back to the patient. No legal liability that way. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judy Collins [JCollins@palmbeachpath.com] Sent: Tuesday, November 25, 2008 9:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: As Thanksgiving Approaches One thing to keep in mind about Vinegar, however, is that fungus will still grow in it after a while. Kind of gross! Judy Collins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Nov 25 10:04:46 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Nov 25 10:04:44 2008 Subject: [Histonet] California State Regulations In-Reply-To: <57BE698966D5C54EAE8612E8941D76830441047B@EXCHANGE3.huntingtonhospital.com> Message-ID: Medi-Cal requires that the many of the records be kept 3 years. "Laurie Colbert" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/25/2008 07:19 AM To cc Subject [Histonet] California State Regulations I'm looking for information regarding hospital labs in California. I need to know if Calif state regulations are more strict than CAP regarding record retention. CAP says we need to keep logs for 2 years. I'm getting varying info on state regs - 3 years, 6 years, 10 years. I am wondering how long other labs are keeping accession logs, IHC logs, special stain/IHC requests, etc. Thanks in advance. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Nov 25 10:16:23 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Nov 25 10:16:29 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think abouta gall bladder in vinegar ? In-Reply-To: References: <16C83872A53F4346AA9C3A18E3A3AAB903F76E95@VHAV10MSGA1.v10.med.va.gov> Message-ID: <01FFE313D8BE4110A6A97A6A9BB9A281@Patsyoffice> I would think that embedding the whole thing in paraffin would be the best approach. They have to deal with this issue in the UK and they are required to give tissue back to anyone who wants it now. I visited a lab there where they were making little wooden coffin boxes to hold paraffin blocks. The patient should be charged for the tissue processing. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, November 25, 2008 8:17 AM To: 'Weber, Susan (VHACLE)'; histonet Subject: RE: [Histonet] as Thanksgiving approaches, what do you think abouta gall bladder in vinegar ? I'm overwhelmed at the huge response. Thank you all. The patient was instructed to make arrangements with a funeral home. The patient is 29 yrs old and since the funeral home might be taking on the responsibility of storing for several decades, she's been unsuccessful in identifying one willing to assist her. I don't have any way to know what the mom's appendix is in. it's quite possible she obtained it before the regs on formaldehyde became so restrictive (mid- '80's I believe). The vinegar was thought to be a solution that would not grow organisms. The gall bladder has been cut down and now consists of a few very thin strips so I was concerned that allowing the tissue to air dry may appear (to the patient) that the specimen had been compromised. Have a great holiday everyone, and thank you again. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: Weber, Susan (VHACLE) [mailto:Susan.Weber2@va.gov] Sent: Tuesday, November 25, 2008 9:07 AM To: Della Speranza, Vinnie; histonet Subject: RE: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? Does she already have a funeral home picked out? Perhaps she can ask the funeral home to "store" it for her, and then release it only to a funeral home. I would consult my legal department to see what they feel is appropriate, that way you are dotting all your t's and crossing your eyes >.< as well! Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Monday, November 24, 2008 5:37 PM To: histonet Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jacqueline.Farnsworth <@t> cls.ab.ca Tue Nov 25 10:12:16 2008 From: Jacqueline.Farnsworth <@t> cls.ab.ca (Jacqueline Farnsworth) Date: Tue Nov 25 10:17:14 2008 Subject: [Histonet] RE: Re: As Thanksgiving Approaches In-Reply-To: <938D716CD445614ABBB817517557B6F43A464493@NADCWPMSGCMS09.hca.corpad.net> References: <05CAE76AB5D5ED409864C6DD86F13349024DB5E7B8@pbpsflexch02.pbp.local> <38667E7FB77ECD4E91BFAEB8D98638631D2F0F96E8@LRGHEXVS1.practice.lrgh.org>, <938D716CD445614ABBB817517557B6F43A464493@NADCWPMSGCMS09.hca.corpad.net> Message-ID: We do release specimens to patients (with photo id required) , but we have forms that they must read and sign (releasing us of any potential issues). On the form, we explain the hazards and how to store human tissue, glass slides, paraffin blocks, etc. We rinse all the fresh specimens to remove as much formalin as possible. We keep a copy attached to the report and send a copy with the pt / family member. Jacquie Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vacca Jessica [Jessica.Vacca@HCAhealthcare.com] Sent: November 25, 2008 7:59 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Re: As Thanksgiving Approaches We will only release items to the funeral home, Find out if they already have arrangements with one and release it to them. Otherwise it won't get released. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Tuesday, November 25, 2008 9:51 AM To: Judy Collins; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Re: As Thanksgiving Approaches Once specimens arrive at our lab, they are ours. We do not give anything back to the patient. No legal liability that way. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judy Collins [JCollins@palmbeachpath.com] Sent: Tuesday, November 25, 2008 9:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: As Thanksgiving Approaches One thing to keep in mind about Vinegar, however, is that fungus will still grow in it after a while. Kind of gross! Judy Collins _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From histonetalias <@t> gmail.com Tue Nov 25 11:08:27 2008 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Tue Nov 25 11:08:30 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? In-Reply-To: References: Message-ID: <4b6c85510811250908j286b30wf0b947d14402281@mail.gmail.com> Use brine as a base for all pickling. Mix 6 cups distilled water, 3 cups white vinegar and 1/2 cup pickling salt in a large pot. Boil gently. On Mon, Nov 24, 2008 at 5:37 PM, Della Speranza, Vinnie wrote: > I have a patient requesting her gall bladder be returned to her for > religious reasons. > The premise I've been given is so that, upon death, the patient may be > stored with her body parts. > > My facility has concerns about providing it to her in formalin (for obvious > reasons) or alcohol. The patient admits this is a family practice with > momma's appendix already being stored in the attic. > > It can get a bit toasty warm here in the South so attic storage of a > specimen in alcohol may not be prudent and I can't be absolutely certain it > wouldn't burn the house down, another potential liability for my > institution. > > I'm tempted to give it to her in food grade vinegar, to avoid the potential > liabilities from using anything that could be considered hazardous. > Assuming that returning her gall bladder is a given, what do you think of > using vinegar for this purpose? > > > > Vinnie Della Speranza > > Manager for Anatomic Pathology Services > > Medical University of South Carolina > > 165 Ashley Avenue Suite 309 > > Charleston, South Carolina 29425 > > Tel: (843) 792-6353 > > Fax: (843) 792-8974 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- The Unknown HT(ASCP) From nefff <@t> staff.uni-marburg.de Tue Nov 25 11:33:35 2008 From: nefff <@t> staff.uni-marburg.de (Dr. med. Frauke Neff) Date: Tue Nov 25 11:33:43 2008 Subject: [Histonet] noradrenaline staining Message-ID: <1227634415.492c36ef7fab9@webmail.med.uni-marburg.de> Dear Netters, one researcher wants me to do an immunohistochemistry stain of rat brains for noradrenaline. The data sheet mentioned that the tissue should have been fixed with glutaraldehyde but I just got FFPE and a few snap frozen samples. Is it possible to thaw the frozen samples and fix them with glutaraldehyde or does it produce lots of artifacts? Does anyone have an idea how to go on with the FFPE samples? Does anyone know an alternative stain for noradrenaline? I read something about masson-goldner stain, but I don't know if it is going to work on brains. Thanks in advance, Frauke ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From Mark.Frei <@t> sial.com Tue Nov 25 12:17:34 2008 From: Mark.Frei <@t> sial.com (Mark Frei) Date: Tue Nov 25 12:18:14 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? In-Reply-To: <200811251802.mAPI2vfY058456@stlspfw2.sial.com> Message-ID: At a risk of inviting a series of puns/jokes, I do remember a Journal of Histotechnology article that discussed the use of honey as a fixative and a preservative. Mark Frei MT(ASCP) / Technical Marketing Specialist Sigma-Aldrich Hematology & Histology 3050 Spruce Street / Saint Louis, MO, 63103 / USA P: (800) 771-5765, x4164 / Direct: (314) 286-8080 sigma-aldrich.com This message and any files transmitted with it are the property of Sigma-Aldrich Corporation, are confidential, and are intended solely for the use of the person or entity to whom this e-mail is addressed. If you are not one of the named recipient(s) or otherwise have reason to believe that you have received this message in error, please contact the sender and delete this message immediately from your computer. Any other use, retention, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. From christina.thurby <@t> bms.com Tue Nov 25 12:21:42 2008 From: christina.thurby <@t> bms.com (Christina Thurby) Date: Tue Nov 25 12:21:54 2008 Subject: [Histonet] Re: RE: as Thanksgiving approaches, what do you think about a gallbladder in vinegar ? (Tony Henwood) In-Reply-To: <0KAW00F847JZD2@dakia.bms.com> References: <0KAW00F847JZD2@dakia.bms.com> Message-ID: <492C4236.6020405@bms.com> Tony, I really like this idea of using mineral oil to transfer the specimen back to the patient. I used to work in a hospital and would get requests for tissues just as Vinne has described. Our hospital administration would instruct us on cases where the tissue had to be returned to the patient for religious burial reasons and we would seal the tissue in a bag using a kitchen sealer with no additional fixative present. In the future your legal department may want to draft up a tissue release form that would help eliminate any liabilities for mishandling of the tissue. Christina Thurby 812-429-8097 > 16. RE: as Thanksgiving approaches, what do you think about a > gall bladder in vinegar ? (Tony Henwood) > > >See: Henwood (2002)"Color preservation in pathology museum specimens" >published in Biotech Histochem 2002 Jul; 77(4): 230. > >Regards > >Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) >Laboratory Manager & Senior Scientist >Tel: 612 9845 3306 >Fax: 612 9845 3318 >the children's hospital at westmead >Cnr Hawkesbury Road and Hainsworth Street, Westmead >Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > From litepath2000 <@t> yahoo.com Tue Nov 25 13:16:42 2008 From: litepath2000 <@t> yahoo.com (NYSHistotech) Date: Tue Nov 25 13:16:47 2008 Subject: [Histonet] Fw: Histonet Digest, Vol 60, Issue 41: gall bladder in vinegar Message-ID: <721682.15848.qm@web58802.mail.re1.yahoo.com> what about processing all the way through to paraffin? no toxic chemical, biological or flammability?issues/liability, the tissue is intact and relatively "stable" worst possibility, heat will soften it causing it to deform in the container thinking out loud..... Luis ________________________________ From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 25, 2008 1:07:18 PM Subject: Histonet Digest, Vol 60, Issue 41 Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. Re: as Thanksgiving approaches,??? what do you think about a ? ? ? gall bladder in vinegar ? (Rene J Buesa) ? 2. Re: As Thanksgiving Approaches (Judy Collins) ? 3. RE: as Thanksgiving approaches,??? what do you think about a ? ? ? gall bladder in vinegar ? (Rosa Fields) ? 4. RE: Re: As Thanksgiving Approaches (Podawiltz, Thomas) ? 5. RE: Re: As Thanksgiving Approaches (Edwards, R.E.) ? 6. RE: Re: As Thanksgiving Approaches (Vacca Jessica) ? 7. RE: as Thanksgiving approaches, what do you think about??? a ? ? ? gall bladder in vinegar ? (Della Speranza, Vinnie) ? 8. California State Regulations (Laurie Colbert) ? 9. RE: Re: As Thanksgiving Approaches (Della Speranza, Vinnie) ? 10. RE: as Thanksgiving approaches, what do you think about??? a ? ? ? gall bladder in vinegar ? (Blazek, Linda) ? 11. RE: as Thanksgiving approaches,??? what do you think abouta gall ? ? ? bladder in vinegar ? (Horn, Hazel V) ? 12. RE: Re: As Thanksgiving Approaches (Podawiltz, Thomas) ? 13. Re: RE: Re: As Thanksgiving Approaches (Jennifer MacDonald) ? 14. Re: California State Regulations (Jennifer MacDonald) ? 15. RE: as Thanksgiving approaches,??? what do you think abouta gall ? ? ? bladder in vinegar ? (Patsy Ruegg) ? 16. RE: Re: As Thanksgiving Approaches (Jacqueline Farnsworth) ? 17. Re: as Thanksgiving approaches,??? what do you think about a ? ? ? gall bladder in vinegar ? (Histonet Alias) ? 18. noradrenaline staining (Dr. med. Frauke Neff) From BMolinari <@t> heart.thi.tmc.edu Tue Nov 25 13:17:48 2008 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Tue Nov 25 13:17:52 2008 Subject: FW: [Histonet] honey as a formalin substitute Message-ID: I had kept this in my emails. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Thursday, September 21, 2006 5:07 PM To: Rene J Buesa; Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] honey as a formalin substitute I would also be curious to know if honey collected from plants high in estrogens would affect any type of estrogen staining after processing tissue fixed in honey. Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, September 21, 2006 8:30 AM To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] honey as a formalin substitute Dawn: The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9). I read the article and was not really impressed by it because: 1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary; 2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and 3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey. If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication. Just my opinion based in what I read. Ren? J. "Olszewski, Dawn" wrote: Help!!! We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. Dawn Olszewski SGMC Valdosta, Ga dawn.olszewski@sgmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sally.norton <@t> seattlechildrens.org Tue Nov 25 13:32:48 2008 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Tue Nov 25 13:43:28 2008 Subject: [Histonet] Alcoholic Saffron Message-ID: <16E0693C7018C245959AC729FE66EDE52DBD12@s107.childrens.sea.kids> Hello Histonetters, Does anyone use the Alcoholic Saffron Solution pre-made from Rowley Biochemical Institute? We would like to know how the long the expiration period is for it. We either tossed or didn't receive an MSDS sheet on it. Thank you, Sally Norton, HT Seattle Childrens Hospital Seattle, Wa Children's Hospital and Regional Medical Center is now Seattle Children's. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From pieronelva01 <@t> bigpond.com Tue Nov 25 13:49:41 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Tue Nov 25 13:49:52 2008 Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? References: <07732CE52EC3174AB891DE1C62DB4D8F43ED65@GIEXCHANGE.gidocs.net> Message-ID: <8B8C528005D7419D8F8DAF0C4F14160E@pentium4> We have released patient's tissue with a copy of the relevant MSDS. Piero ----- Original Message ----- From: "Rosa Fields" To: "Della Speranza, Vinnie" ; "histonet" Sent: Wednesday, November 26, 2008 1:40 AM Subject: RE: [Histonet] as Thanksgiving approaches,what do you think about a gall bladder in vinegar ? I would have to agree, the vinegar sounds like a good solution. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Monday, November 24, 2008 4:37 PM To: histonet Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.9.10/1810 - Release Date: 11/24/2008 2:36 PM From Kimberly.Marshall <@t> ahss.org Tue Nov 25 13:58:45 2008 From: Kimberly.Marshall <@t> ahss.org (Marshall, Kimberly) Date: Tue Nov 25 13:59:51 2008 Subject: [Histonet] RE; Thanksgiving /gallbladder in vinegar Message-ID: Howdy all... Its good to see other labs with the issues of specimen going back to the patient. I have twice in the past 4 years had to return a placenta to a patient due to religious ideals. My legal folks seem to feel that the religious reasons out weigh the legal and bio-hazards. No matter what the state law. The vinegar idea is a good one but a whole placenta????? ewwwww. And how are some of you handling <20 week fetus??? Another specimen that people ask for. I would think Funeral homes would handle this but not in all cases. Any ideas????? Thank you and happy Thanskgiving Kimberly Marshall HT (ASCP) ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From AGrobe2555 <@t> aol.com Tue Nov 25 14:05:21 2008 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Tue Nov 25 14:05:29 2008 Subject: [Histonet] Hematoxylin staining and Calcification Message-ID: Hello all, Is there anything published on using H&E staining to presumptively indicate calcification (primarily the hematoxylin)? Thanks, Albert Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana Foundation **************One site has it all. Your email accounts, your social networks, and the things you love. Try the new AOL.com today!(http://pr.atwola.com/promoclk/100000075x1212962939x1200825291/aol?redir=http://www.aol.com/?optin=new-dp %26icid=aolcom40vanity%26ncid=emlcntaolcom00000001) From BFicher <@t> chomp.org Tue Nov 25 14:29:25 2008 From: BFicher <@t> chomp.org (Fischer, R. B) Date: Tue Nov 25 14:29:32 2008 Subject: [Histonet] gram stain controls Message-ID: My pathologist has made a gram neg/pos control block for us to use. He made this by taking separate cultures of each and suspended them in histo gel. The control stains well. My question is: Since we are performing gram stain on tissues exclusively, will this cultured control be an accepted method as a control for tissues, or must we use tissue with gram pos/neg organisms. Thanks for your reply. R.Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box HH Monterey Ca. 93942 831-625-4791 Fax: 831-6583683 Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. From rjbuesa <@t> yahoo.com Tue Nov 25 14:39:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 25 14:39:33 2008 Subject: [Histonet] gram stain controls In-Reply-To: Message-ID: <310575.91456.qm@web65716.mail.ac4.yahoo.com> I always used actual tissues with gram +/- bacteria because it is not the same (time wise) to stain a histogel than a piece of tissue. Appendix is a good control. Ren? J. --- On Tue, 11/25/08, Fischer, R. B wrote: From: Fischer, R. B Subject: [Histonet] gram stain controls To: histonet@lists.utsouthwestern.edu Cc: "Delcambre, Linda V" Date: Tuesday, November 25, 2008, 3:29 PM My pathologist has made a gram neg/pos control block for us to use. He made this by taking separate cultures of each and suspended them in histo gel. The control stains well. My question is: Since we are performing gram stain on tissues exclusively, will this cultured control be an accepted method as a control for tissues, or must we use tissue with gram pos/neg organisms. Thanks for your reply. R.Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box HH Monterey Ca. 93942 831-625-4791 Fax: 831-6583683 Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Tue Nov 25 15:02:47 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Nov 25 15:02:56 2008 Subject: [Histonet] Can you believe it Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635B2B@hpes1.HealthPartners.int> Has anyone ever heard of cassettes and tissue totally disintegrating in formic acid decal solution with a high heat on the solution for about 3 hours????? We had 3 cassettes with tissue for decal placed in our container on the platform for adding agitation. Someone had moved the knob to heat and this wasn't noticed until about 2-3 hours later, when it was boiling! We cannot locate the cassettes (I think someone misplaced them) and others think they totally disintegrated with the decal and heat...what does everyone think????? In all of my years of histology, never have I heard of anything so weird ...... Dorothy Webb ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From stephanie.d.rivera <@t> gsk.com Tue Nov 25 15:15:31 2008 From: stephanie.d.rivera <@t> gsk.com (stephanie.d.rivera@gsk.com) Date: Tue Nov 25 15:15:54 2008 Subject: [Histonet] Can you believe it In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2705635B2B@hpes1.HealthPartners.int> Message-ID: I can believe it! I have heard of the tissue disentergrating in decal(CAL-RITE) with heat. The stirrer was on and someone hit the heat knob and this was on a Friday. Well, on Monday there was no tissue, the cassette was chewed but it was there. Now we have tape across the heat knobs on all stirrer plates. Stephanie D. Rivera phone: 610-270-7340 "Webb, Dorothy L" Sent by: histonet-bounces@lists.utsouthwestern.edu 25-Nov-2008 16:02 To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Can you believe it Has anyone ever heard of cassettes and tissue totally disintegrating in formic acid decal solution with a high heat on the solution for about 3 hours????? We had 3 cassettes with tissue for decal placed in our container on the platform for adding agitation. Someone had moved the knob to heat and this wasn't noticed until about 2-3 hours later, when it was boiling! We cannot locate the cassettes (I think someone misplaced them) and others think they totally disintegrated with the decal and heat...what does everyone think????? In all of my years of histology, never have I heard of anything so weird ...... Dorothy Webb ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Tue Nov 25 15:26:05 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Nov 25 15:26:11 2008 Subject: [Histonet] Re: As Thanksgiving Approaches Message-ID: I'd think that v?sicule biliaire vinaigrette would be inclined to get the moldies. Of several not very satisfactory solutions, Tony Henwood's suggestion of mineral oil (paraffin oil) might be the safest, though messy if it gets spilled. What religion requires decades-long preservation of gallbladders? Highly observant Jews sometimes request return of tissues, but their requirement is that the tissue be buried in a Jewish cemetery - as soon as possible, not waiting for the rest of the patient to arrive. Do Muslims have any issues here? - I'm not aware of any Christian tradition that has any rules about this problem. In my personal experience, the most common problem of this sort has been the patient who wants an amputated leg buried with him. Whenever I've dealt with this problem, a funeral director has bailed me out. As far as I know, there was no religious issue with the legs, just personal (or cultural) preference. The most bizarre situation of this sort happened to me about ten years ago. A rural midwife had asked an OB-GYN to remove a retained placenta after a difficult delivery. The OB-GYN put the placenta in formalin and sent it to a pathology service some distance away. The midwife called the lab, and was furious to learn that the placenta had been put in formalin. It seems that (I hope you're not reading your e-mail with lunch) the midwife had her patients eat their babies' placentas. I think the JCAHO or somebody banned returning gallstones to patients, a practice that used to be quite a nuisance for pathologists. Bob Richmond Samurai Pathologist Knoxville TN From bliven.laura <@t> marshfieldclinic.org Tue Nov 25 15:35:24 2008 From: bliven.laura <@t> marshfieldclinic.org (Bliven, Laura) Date: Tue Nov 25 15:35:29 2008 Subject: [Histonet] Microwave Histoprocessor for Sale Message-ID: <200811252135.mAPLZPx5012234@mailhost2.mfldclin.edu> Microwave Vacuum Histoprocessor for Sale Milestone RHS-1 Purchased 8-30-05 Original Purchased Price: $42,000 Best Offered Accepted Condition - Excellent, rarely used Complete system, including manual Available - Immediately upon receipt of payment in full For information, please contact: spath.judith@marshfieldclinic.org Thanks Laura Bliven, AAS, HT(ASCP), QIHC Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 From carrolpb <@t> umdnj.edu Tue Nov 25 15:36:29 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Tue Nov 25 15:38:57 2008 Subject: [Histonet] Re: As Thanksgiving Approaches In-Reply-To: References: Message-ID: <492C6FDD.8030805@umdnj.edu> > the midwife had her patients eat their babies' placentas It seems that placentaophagy is comparatively (and increasingly) common... http://en.wikipedia.org/wiki/Placentophagy From AnthonyH <@t> chw.edu.au Tue Nov 25 16:14:45 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Nov 25 16:14:58 2008 Subject: [Histonet] as Thanksgiving approaches,what do you think abouta gall bladder in vinegar ? In-Reply-To: <01FFE313D8BE4110A6A97A6A9BB9A281@Patsyoffice> Message-ID: Another option is to dehydrate the specimen (ethanol or acetone)and infiltrate with one of the resins (I have used GMA in the past) allow it to set and hand it over to the patient. They can even mount it on a small wooden pedestal (The "Family Sports Trophy"??- Oh this is getting sad!) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Wednesday, 26 November 2008 3:16 AM To: 'Della Speranza, Vinnie'; 'Weber, Susan (VHACLE)'; 'histonet' Subject: RE: [Histonet] as Thanksgiving approaches,what do you think abouta gall bladder in vinegar ? I would think that embedding the whole thing in paraffin would be the best approach. They have to deal with this issue in the UK and they are required to give tissue back to anyone who wants it now. I visited a lab there where they were making little wooden coffin boxes to hold paraffin blocks. The patient should be charged for the tissue processing. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, November 25, 2008 8:17 AM To: 'Weber, Susan (VHACLE)'; histonet Subject: RE: [Histonet] as Thanksgiving approaches, what do you think abouta gall bladder in vinegar ? I'm overwhelmed at the huge response. Thank you all. The patient was instructed to make arrangements with a funeral home. The patient is 29 yrs old and since the funeral home might be taking on the responsibility of storing for several decades, she's been unsuccessful in identifying one willing to assist her. I don't have any way to know what the mom's appendix is in. it's quite possible she obtained it before the regs on formaldehyde became so restrictive (mid- '80's I believe). The vinegar was thought to be a solution that would not grow organisms. The gall bladder has been cut down and now consists of a few very thin strips so I was concerned that allowing the tissue to air dry may appear (to the patient) that the specimen had been compromised. Have a great holiday everyone, and thank you again. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: Weber, Susan (VHACLE) [mailto:Susan.Weber2@va.gov] Sent: Tuesday, November 25, 2008 9:07 AM To: Della Speranza, Vinnie; histonet Subject: RE: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? Does she already have a funeral home picked out? Perhaps she can ask the funeral home to "store" it for her, and then release it only to a funeral home. I would consult my legal department to see what they feel is appropriate, that way you are dotting all your t's and crossing your eyes >.< as well! Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Monday, November 24, 2008 5:37 PM To: histonet Subject: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ? I have a patient requesting her gall bladder be returned to her for religious reasons. The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Annette.Fletcher <@t> providence.org Tue Nov 25 16:35:59 2008 From: Annette.Fletcher <@t> providence.org (Fletcher, Annette M) Date: Tue Nov 25 16:36:04 2008 Subject: [Histonet] Histotech Opportunity in Oregon Message-ID: <284596E350CB0743BB500B18475E7A0B018ABB5C@wn1223.or.providence.org> Good Afternoon Histonetters, Providence Health and Services in Portland, OR is seeking a Certified, preferably Registered Histotech to work a full-time, night shift position at one of our premier Hosptials. Our compensation is highly competitive and we offer an excellent benefits package. You can review our job description by going to www.providence.org, click on Careers and then Job Search. You will be directed to a page that asks for search criteria. Scroll down the field that asks for a key word or requisition and type in 44459, then scroll down and click "search". The job title will appear. You can click on the title to open the description and apply if you like. You can email me directly at annette.fletcher@providence.org or call me at 503-215-5840. No Recruiters please. Kind Regards, Annette M Fletcher Senior Recruiter Providence Health & Services, Employment 1235 NE 47th Avenue - Suite 200 Portland, OR 97213 T: 503-215-5840 F: 503-215-4770 Annette.fletcher@providence.org DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From CIngles <@t> uwhealth.org Tue Nov 25 16:50:35 2008 From: CIngles <@t> uwhealth.org (Ingles Claire ) Date: Tue Nov 25 16:51:16 2008 Subject: [Histonet] Re: As Thanksgiving Approaches References: Message-ID: <08A0A863637F1349BBFD83A96B27A50A1201BD@uwhis-xchng3.uwhis.hosp.wisc.edu> Scientology? What religion requires decades-long preservation of gallbladders? Highly observant Jews sometimes request return of tissues, but their requirement is that the tissue be buried in a Jewish cemetery - as soon as possible, not waiting for the rest of the patient to arrive. Do Muslims have any issues here? - I'm not aware of any Christian tradition that has any rules about this problem. From contact <@t> excaliburpathology.com Tue Nov 25 17:00:36 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Nov 25 17:00:40 2008 Subject: [Histonet] Re: As Thanksgiving Approaches Message-ID: <96466.51312.qm@web1102.biz.mail.sk1.yahoo.com> Native Americans. ? ________________________________ From: Ingles Claire To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 25, 2008 4:50:35 PM Subject: RE: [Histonet] Re: As Thanksgiving Approaches Scientology? What religion requires decades-long preservation of gallbladders? Highly observant Jews sometimes request return of tissues, but their requirement is that the tissue be buried in a Jewish cemetery - as soon as possible, not waiting for the rest of the patient to arrive. Do Muslims have any issues here? - I'm not aware of any Christian tradition that has any rules about this problem. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Tue Nov 25 17:09:41 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Nov 25 17:09:46 2008 Subject: [Histonet] Re: As Thanksgiving Approaches In-Reply-To: References: Message-ID: Patient is listed in our system as Presbyterian. I'm guessing that patients know that if they cite religion as the basis for their request they are less likely to be denied. I like the mineral oil and glycerin suggestions as they are probably the least problematic from a safety perspective. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Tuesday, November 25, 2008 4:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: As Thanksgiving Approaches I'd think that v?sicule biliaire vinaigrette would be inclined to get the moldies. Of several not very satisfactory solutions, Tony Henwood's suggestion of mineral oil (paraffin oil) might be the safest, though messy if it gets spilled. What religion requires decades-long preservation of gallbladders? Highly observant Jews sometimes request return of tissues, but their requirement is that the tissue be buried in a Jewish cemetery - as soon as possible, not waiting for the rest of the patient to arrive. Do Muslims have any issues here? - I'm not aware of any Christian tradition that has any rules about this problem. In my personal experience, the most common problem of this sort has been the patient who wants an amputated leg buried with him. Whenever I've dealt with this problem, a funeral director has bailed me out. As far as I know, there was no religious issue with the legs, just personal (or cultural) preference. The most bizarre situation of this sort happened to me about ten years ago. A rural midwife had asked an OB-GYN to remove a retained placenta after a difficult delivery. The OB-GYN put the placenta in formalin and sent it to a pathology service some distance away. The midwife called the lab, and was furious to learn that the placenta had been put in formalin. It seems that (I hope you're not reading your e-mail with lunch) the midwife had her patients eat their babies' placentas. I think the JCAHO or somebody banned returning gallstones to patients, a practice that used to be quite a nuisance for pathologists. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Wed Nov 26 01:10:23 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Nov 26 01:10:28 2008 Subject: [Histonet] Can you believe it In-Reply-To: References: <0E394B648E5284478A6CCB78E5AFDA2705635B2B@hpes1.HealthPartners.int> Message-ID: Interestingly, just yesterday I had a Tissue tek biopsy bag disintigrate in the Sakura electrolytic decalcifier, but only the one nearest the (?) -ve terminal. I thought those things were some sort of nylon and indestructible. Shows, you live and learn! On 11/25/08, stephanie.d.rivera@gsk.com wrote: > > I can believe it! > > I have heard of the tissue disentergrating in decal(CAL-RITE) with heat. > The stirrer was on and someone hit the heat knob and this was on a Friday. > Well, on Monday there was no tissue, the cassette was chewed but it was > there. Now we have tape across the heat knobs on all stirrer plates. > > > > > Stephanie D. Rivera > > phone: 610-270-7340 > > > > > "Webb, Dorothy L" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 25-Nov-2008 16:02 > > To > histonet@lists.utsouthwestern.edu > cc > > Subject > [Histonet] Can you believe it > > > > > > > Has anyone ever heard of cassettes and tissue totally disintegrating in > formic acid decal solution with a high heat on the solution for about 3 > hours????? We had 3 cassettes with tissue for decal placed in our > container on the platform for adding agitation. Someone had moved the > knob to heat and this wasn't noticed until about 2-3 hours later, when > it was boiling! We cannot locate the cassettes (I think someone > misplaced them) and others think they totally disintegrated with the > decal and heat...what does everyone think????? In all of my years of > histology, never have I heard of anything so weird ...... > > Dorothy Webb > ________________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Susan.Walzer <@t> HCAHealthcare.com Wed Nov 26 02:16:58 2008 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Wed Nov 26 02:17:03 2008 Subject: [Histonet] gram stain controls In-Reply-To: <310575.91456.qm@web65716.mail.ac4.yahoo.com> References: <310575.91456.qm@web65716.mail.ac4.yahoo.com> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2A9171F3D5@FWDCWPMSGCMS09.hca.corpad.net> We take two pieces of fresh tissue (umbilical cord works well) to micro and have them grow positive and negative on individual pieces. After a few days we fix the tissue and embed them together. Works great. PS we do the same when we need fungus controls. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 25, 2008 3:40 PM To: histonet@lists.utsouthwestern.edu; Fischer, R. B Cc: Delcambre, Linda V Subject: Re: [Histonet] gram stain controls I always used actual tissues with gram +/- bacteria because it is not the same (time wise) to stain a histogel than a piece of tissue. Appendix is a good control. Ren? J. --- On Tue, 11/25/08, Fischer, R. B wrote: From: Fischer, R. B Subject: [Histonet] gram stain controls To: histonet@lists.utsouthwestern.edu Cc: "Delcambre, Linda V" Date: Tuesday, November 25, 2008, 3:29 PM My pathologist has made a gram neg/pos control block for us to use. He made this by taking separate cultures of each and suspended them in histo gel. The control stains well. My question is: Since we are performing gram stain on tissues exclusively, will this cultured control be an accepted method as a control for tissues, or must we use tissue with gram pos/neg organisms. Thanks for your reply. R.Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box HH Monterey Ca. 93942 831-625-4791 Fax: 831-6583683 Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Wed Nov 26 02:19:34 2008 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Wed Nov 26 02:19:45 2008 Subject: [Histonet] RE: bone saws? In-Reply-To: References: Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2A9171F3D6@FWDCWPMSGCMS09.hca.corpad.net> We use a Fleetwood (used by meat cutters) then have maintenance attach a plexi-glass shield. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqueline Farnsworth Sent: Monday, November 24, 2008 3:30 PM To: Histonet Subject: [Histonet] bone saws? Greetings I am wondering if anyone can share how they are cutting femoral heads, extremities for tumour, etc. We currently use a 20 yr + meat saw (band saw), but would like to find something safer/newer. We freeze all our bones (pre-fixation and pre-decalcification) prior to cutting. Vendors welcome to reply. Thanks in advance Jacquie Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From benoit.delatour <@t> u-psud.fr Wed Nov 26 02:38:04 2008 From: benoit.delatour <@t> u-psud.fr (=?ISO-8859-1?Q?Beno=EEt_Delatour?=) Date: Wed Nov 26 02:38:04 2008 Subject: [Histonet] Epson v750 pro scanner Message-ID: <492D0AEC.3090909@u-psud.fr> Dear Histoneters, Has anyone ever used the Epson v750 pro flatbed scanner to digitize microscopic slides? In the past we get used to a Nikon coolscan scanner and got nice results. We then bought the Epson scanner because of its high spatial resolution (6400 optical dpi) but did not obtain satisfactory images, principally because of bad focusing troubles. Does anyone has a good experience / expertise with this scanner and an efficient protocol to numerize slides with this device? Thanks to all, B. Delatour From pruegg <@t> ihctech.net Wed Nov 26 07:37:11 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Nov 26 07:37:14 2008 Subject: [Histonet] RE: [IHCRG] Re: Microwave Histoprocessor for Sale In-Reply-To: Message-ID: I was wondering the exact same thing? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of michael.ho@sickkids.ca Sent: Wednesday, November 26, 2008 6:22 AM To: bliven.laura@marshfieldclinic.org Cc: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com Subject: [IHCRG] Re: Microwave Histoprocessor for Sale Hi Laura Interested to know why its for sale, is it because the technology is not good or that you are replacing with another type of unit???? Are you fixing as well as processing using this technology???? regards Michael Ho, MLT Resource Technologist Immunopathology laboratory Division of Pathology DPLM The Hospital for Sick Children Toronto, Ontario Canada Tel#:416-813-5950 Fax: 416-813-5974 "Bliven, Laura" "histonet@lists.utsouthwestern.edu" Sent by: ihcrg@googlegroup , "ihcrg@googlegroups.com" s.com cc 11/25/2008 04:35 Subject PM [IHCRG] Microwave Histoprocessor for Sale Please respond to bliven.laura@mars hfieldclinic.org Microwave Vacuum Histoprocessor for Sale Milestone RHS-1 Purchased 8-30-05 Original Purchased Price: $42,000 Best Offered Accepted Condition - Excellent, rarely used Complete system, including manual Available - Immediately upon receipt of payment in full For information, please contact: spath.judith@marshfieldclinic.org Thanks Laura Bliven, AAS, HT(ASCP), QIHC Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- From contact <@t> excaliburpathology.com Wed Nov 26 07:59:54 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Wed Nov 26 07:59:59 2008 Subject: [Histonet] Epson v750 pro scanner Message-ID: <749109.6185.qm@web1111.biz.mail.sk1.yahoo.com> I recently purchased the newest model of the Meyer Pathscan Enabler. Model IV. http://www.meyerinst.com/html/oem/pse4/default.htm It is great for low mag pics, super easy to use, and the price could not be beat. ________________________________ From: Beno?t Delatour To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 26, 2008 2:38:04 AM Subject: [Histonet] Epson v750 pro scanner Dear Histoneters, Has anyone ever used the Epson v750 pro flatbed scanner to digitize microscopic slides? In the past we get used to a Nikon coolscan scanner and got nice results. We then bought the Epson scanner because of its high spatial resolution (6400 optical dpi) but did not obtain satisfactory images, principally because of bad focusing troubles. Does anyone has a good experience / expertise with this scanner and an efficient protocol to numerize slides with this device? Thanks to all, B. Delatour _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Wed Nov 26 08:33:14 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Nov 26 08:33:21 2008 Subject: [Histonet] Re: As Thanksgiving Approaches In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82F1E@EMAIL.archildrens.org> I am a Presbyterian and I have never heard of this. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Tuesday, November 25, 2008 5:10 PM To: 'Robert Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: As Thanksgiving Approaches Patient is listed in our system as Presbyterian. I'm guessing that patients know that if they cite religion as the basis for their request they are less likely to be denied. I like the mineral oil and glycerin suggestions as they are probably the least problematic from a safety perspective. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Tuesday, November 25, 2008 4:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: As Thanksgiving Approaches I'd think that v?sicule biliaire vinaigrette would be inclined to get the moldies. Of several not very satisfactory solutions, Tony Henwood's suggestion of mineral oil (paraffin oil) might be the safest, though messy if it gets spilled. What religion requires decades-long preservation of gallbladders? Highly observant Jews sometimes request return of tissues, but their requirement is that the tissue be buried in a Jewish cemetery - as soon as possible, not waiting for the rest of the patient to arrive. Do Muslims have any issues here? - I'm not aware of any Christian tradition that has any rules about this problem. In my personal experience, the most common problem of this sort has been the patient who wants an amputated leg buried with him. Whenever I've dealt with this problem, a funeral director has bailed me out. As far as I know, there was no religious issue with the legs, just personal (or cultural) preference. The most bizarre situation of this sort happened to me about ten years ago. A rural midwife had asked an OB-GYN to remove a retained placenta after a difficult delivery. The OB-GYN put the placenta in formalin and sent it to a pathology service some distance away. The midwife called the lab, and was furious to learn that the placenta had been put in formalin. It seems that (I hope you're not reading your e-mail with lunch) the midwife had her patients eat their babies' placentas. I think the JCAHO or somebody banned returning gallstones to patients, a practice that used to be quite a nuisance for pathologists. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From tbraud <@t> holyredeemer.com Wed Nov 26 09:43:27 2008 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Nov 26 09:43:33 2008 Subject: [Histonet] RE: Histonet Digest, Return of Body Parts for religious reasons In-Reply-To: <1ebed29a0001ceee@HolyRedeemer.com> Message-ID: You might want to check with local regulations concerning burial. Here, and at other facilities that I've been a part of, we receive these requests regularly, usually associated with amputated limbs, however, we make it a policy to release no "soft tissue" to a patient, but instead we ask that they make arrangements with a funeral home (since this is supposed to be for purposes of burial, eventually) and it is only to the funeral home that we will release the part. What happens to the part after that, is between the patient and their funeral home representative, which is much more familiar with regulations and practices for these situations. This is the only way to complete way protect the hospital from liability of having a body part "stored in the attic". Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Aubrey <@t> nsh.org Wed Nov 26 10:13:12 2008 From: Aubrey <@t> nsh.org (Aubrey Wanner) Date: Wed Nov 26 10:13:17 2008 Subject: [Histonet] NSH Holiday Lunch & Learn Message-ID: Spaces still available for the NSH Holiday Lunch & Learn scheduled for December 6th in St. Louis. If you are interested in the opportunity to earn 4.5 contact hours by the end of the year, the Holiday Lunch and Learn is here to help. Sessions for the NSH Holiday Lunch & Learn, presented in partnership with Leica Microsystems, are: Schedule: 8:00am - 9:00am: Registration 9:00am - 10:30am: Small Specimen Management - In a Large Volume World 10:30am - 12:00am: Immunohistochemical Staining Methods from Beginning to End 12:00pm - 12:30pm: Lunch Provided by NSH 12:30pm - 2:00pm: The Paradox of Change in Histotechnology Sessions are presented by Skip Brown, Leica BioSystems L.L.C.-St. Louis and Debra Grant, Leica Microsystems. Visit our website: Register Online We hope to see you there! Call the NSH Office with any questions, 443-535-4060. From Tracey.Lenek <@t> cls.ab.ca Wed Nov 26 10:59:04 2008 From: Tracey.Lenek <@t> cls.ab.ca (Tracey Lenek) Date: Wed Nov 26 10:59:09 2008 Subject: [Histonet] Ventana Benchmark XTs Message-ID: <2DFAEEFF192A9141ABACFF88BE613BF30BBC56@EXMBXC1.crha.bewell.ca> Hi, We have had on-going thermal slide pad issues with our XTs since they were installed in February. The slide tray assemblies have been replaced not once but twice and we are still having inconsistent results with the temp verifier slides. Has anyone experienced the same issues with this instrumentation? Thanks Tracey Lenek Tech III - Anatomic Pathology Calgary Laboratory Services 403-770-3588 ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From CHRISH <@t> HEALTHCARESCOUTS.COM Wed Nov 26 12:03:32 2008 From: CHRISH <@t> HEALTHCARESCOUTS.COM (Chris Handrahan) Date: Wed Nov 26 12:11:54 2008 Subject: [Histonet] Happy Thanksgiving from Healthcare Scouts Message-ID: Healthcare Scouts would like to extend the best wishes for a healthy and relaxing holiday! Healthcare Scouts specializes in nationwide permanent placement of highly qualified healthcare professionals who value excellence and high quality patient care. Offering a unique blend of specialization and scope of services, Healthcare Scouts places pharmacy, nurse case management, radiology, respiratory therapy, and lab/bio tech professionals. Chris Handrahan Managing Director of Allied Health Healthcare Scouts 800-708-0605 office 321-231-5427 cell chrish@healthcarescouts.com www.healthcarescouts.com From petepath <@t> yahoo.com Wed Nov 26 12:53:43 2008 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Wed Nov 26 12:53:48 2008 Subject: [Histonet] bone saws Message-ID: <37310.70525.qm@web45103.mail.sp1.yahoo.com> Dear Jacquie, ? We have been using a combination of?a $100 power hand saw made by Bosch with a vice ?I origially made for our labs use. After 4 years of success in our lab I have designed a "bone vice"?for?tightly holding bone specimens while using this inexpensive saw. When ?I originally introduced my prototype to our lab my PA came back to me saying I ?just finished 2 hours work in 20 minutes. With this combo you can completely slice a femoral head?up like a hard boiled egg in a minute or two. Cutting the head in half takes seconds.?My vice is designed agter molding femoral heads and creating several ?V shaped notched to hold the round surfaces. It is fixed to 1x1 ft corion platform and can be placed in the sink for cleaning. The link below will take you to my web page where you will see videos of this saw and vice. The saw is a easy to handle small power saw with a thin kerf. I believe this combo is a great deal safer than the band saws so many are using. I sell only the vice. The saw can be purchased from CPO bosch for the price of a disposible. Replacement blades are $15 and last for a ?long time. We change ours about once a year. ? http://pathologyinnovations.com/bone_vise.htm Stephen Peters M.D. Vice Chairman?of Pathology Hackensack University Medical Center 201 996 4836 ? Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From paul.kammeyer <@t> comphealth.com Wed Nov 26 12:59:07 2008 From: paul.kammeyer <@t> comphealth.com (paul.kammeyer@comphealth.com) Date: Wed Nov 26 12:59:15 2008 Subject: [Histonet] Happy Thanksgiving from the Team at CompHealth Message-ID: CompHealth would like to wish everyone a Happy and Safe Thanksgiving! Get in touch with us anytime and let us help you with your laboratory staffing needs. We concentrate on Permanent placement as well as Contract/Travel work. We would be happy to talk with you about what we can offer. Contact us by phone, email or visit our website at www.comphealth.com. If you are interested in learning more about contract/travel work get in touch with me. We are receiving positions almost daily and need qualified techs to fill them. In 1979, CompHealth was awarded a federal grant to organize short-term physician staffing services for needy areas throughout the western United States. These services were so successful that healthcare organizations across the country began requesting temporary physician coverage. The locum tenens industry was born! For the first ten years, CompHealth focused exclusively on temporary physician staffing services and became the largest locum tenens staffing firm in the U.S. From our leadership position in locum tenens, CompHealth broadened its business lines to become the leading national provider of allied health professionals, both temporary staffing and permanent placement. Today, CompHealth is the best single resource for healthcare professionals seeking employment and for healthcare organizations seeking complete recruiting and staffing services. Paul Kammeyer Staffing Consultant Lab Specialties - CompHealth P.O. Box 713400 Salt Lake City, UT 84171-3400 Phone: (800) 447-6016 ext. 3380 Fax: (866) 588-1340, (801) 930-4504 paul.kammeyer@comphealth.com www.comphealth.com Ask me about our $500 Referral Bonus Program!! From denise.woodward <@t> uconn.edu Wed Nov 26 13:03:23 2008 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Wed Nov 26 13:03:28 2008 Subject: FW: [Histonet] Can you believe it Message-ID: <40AC6D73C2B95C4CA21B26B7BF380C4002D0E4A1@EXCHANGED.mgmt.ad.uconn.edu> -----Original Message----- From: Woodward, Denise Sent: Wednesday, November 26, 2008 2:00 PM To: 'Webb, Dorothy L' Subject: RE: [Histonet] Can you believe it Yes I Have heard of total disintegration. A histologist friend in Maine told me about having to take the heat knob off a combination heat/stir plate when the heat knob was turned on instead of the stir knob and the cassettes of bone to be decalcified completely disintegrated by morning. This happened twice. The heat knob was removed before the third occurrence could transpire! Denise Long Woodward -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, November 25, 2008 4:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Can you believe it Has anyone ever heard of cassettes and tissue totally disintegrating in formic acid decal solution with a high heat on the solution for about 3 hours????? We had 3 cassettes with tissue for decal placed in our container on the platform for adding agitation. Someone had moved the knob to heat and this wasn't noticed until about 2-3 hours later, when it was boiling! We cannot locate the cassettes (I think someone misplaced them) and others think they totally disintegrated with the decal and heat...what does everyone think????? In all of my years of histology, never have I heard of anything so weird ...... Dorothy Webb ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Wed Nov 26 14:31:51 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Nov 26 14:31:58 2008 Subject: [Histonet] Can you believe it In-Reply-To: <40AC6D73C2B95C4CA21B26B7BF380C4002D0E4A1@EXCHANGED.mgmt.ad.uconn.edu> References: <40AC6D73C2B95C4CA21B26B7BF380C4002D0E4A1@EXCHANGED.mgmt.ad.uconn.edu> Message-ID: Yes, this happened in our lab also. It was overnight and not 3 hours but it was all gone in the AM. We weren't even allowed to keep the stir plate after that one. Sheila Adey HT MLT Port Huron Hospital Michigan> Date: Wed, 26 Nov 2008 14:03:23 -0500> From: denise.woodward@uconn.edu> To: histonet@lists.utsouthwestern.edu> Subject: FW: [Histonet] Can you believe it> > > > -----Original Message-----> From: Woodward, Denise > Sent: Wednesday, November 26, 2008 2:00 PM> To: 'Webb, Dorothy L'> Subject: RE: [Histonet] Can you believe it> > Yes I Have heard of total disintegration. > A histologist friend in Maine told me about having to take the heat knob> off a combination heat/stir plate when the heat knob was turned on> instead of the stir knob and the cassettes of bone to be decalcified> completely disintegrated by morning. This happened twice. The heat knob> was removed before the third occurrence could transpire!> Denise Long Woodward> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb,> Dorothy L> Sent: Tuesday, November 25, 2008 4:03 PM> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Can you believe it> > Has anyone ever heard of cassettes and tissue totally disintegrating in> formic acid decal solution with a high heat on the solution for about 3> hours????? We had 3 cassettes with tissue for decal placed in our> container on the platform for adding agitation. Someone had moved the> knob to heat and this wasn't noticed until about 2-3 hours later, when> it was boiling! We cannot locate the cassettes (I think someone> misplaced them) and others think they totally disintegrated with the> decal and heat...what does everyone think????? In all of my years of> histology, never have I heard of anything so weird ......> > Dorothy Webb> ________________________________________> This e-mail and any files transmitted with it are confidential and are> intended solely for the use of the individual or entity to whom they are> addressed. If you are not the intended recipient or the individual> responsible for delivering the e-mail to the intended recipient, please> be advised that you have received this e-mail in error and that any use,> dissemination, forwarding, printing, or copying of this e-mail is> strictly prohibited.> > If you have received this e-mail in error, please immediately notify the> HealthPartners Support Center by telephone at (952) 967-6600. You will> be reimbursed for reasonable costs incurred in notifying us.> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ From carrolpb <@t> umdnj.edu Wed Nov 26 15:24:04 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Wed Nov 26 15:28:24 2008 Subject: [Histonet] Happy Thanksgiving from the Team at CompHealth In-Reply-To: References: Message-ID: <492DBE74.5040404@umdnj.edu> > CompHealth would like to wish everyone a Happy and Safe Thanksgiving! and *I* would like to wish that CompHealth wouldn't spam us in the form of Thanksgiving well-wishes. paul.kammeyer@comphealth.com wrote: > CompHealth would like to wish everyone a Happy and Safe Thanksgiving! > > Get in touch with us anytime and let us help you with your laboratory > staffing needs. We concentrate on Permanent placement as well as > Contract/Travel work. > We would be happy to talk with you about what we can offer. Contact us by > phone, email or visit our website at www.comphealth.com. > > If you are interested in learning more about contract/travel work get in > touch with me. We are receiving positions almost daily and need qualified > techs to fill them. > > In 1979, CompHealth was awarded a federal grant to organize short-term > physician staffing services for needy areas throughout the western United > States. These services were so successful that healthcare organizations > across the country began requesting temporary physician coverage. The > locum tenens industry was born! > For the first ten years, CompHealth focused exclusively on temporary > physician staffing services and became the largest locum tenens staffing > firm in the U.S. From our leadership position in locum tenens, CompHealth > broadened its business lines to become the leading national provider of > allied health professionals, both temporary staffing and permanent > placement. > > Today, CompHealth is the best single resource for healthcare professionals > seeking employment and for healthcare organizations seeking complete > recruiting and staffing services. > > > > Paul Kammeyer > Staffing Consultant > Lab Specialties - CompHealth > P.O. Box 713400 > Salt Lake City, UT 84171-3400 > Phone: (800) 447-6016 ext. 3380 > Fax: (866) 588-1340, (801) 930-4504 > paul.kammeyer@comphealth.com > www.comphealth.com > > Ask me about our $500 Referral Bonus Program!! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From AnthonyH <@t> chw.edu.au Wed Nov 26 15:50:19 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Nov 26 15:50:35 2008 Subject: [Histonet] RE: Histonet Digest, Return of Body Parts for religious reasons In-Reply-To: Message-ID: Amazing how different areas have different regs (or lack of) I thought I would attach our policy, seems to work and, drafted with the best intentions, hopefully prevents litigation and meets the needs of patients: BIOPSY SPECIMENS: RETURNED TO PATIENTS PROCEDURE (c) Background At present, very few patients request the return of resected organs or other samples. When patients are specifically informed of what happens to such specimens, it is likely that such requests will increase in frequency, especially in relation to larger samples including whole organs, the disposal or retention of which may have emotional implications. Laboratories should have established protocols to permit compliance with such requests while minimising any risk to the patient or others from infection or toxic chemicals. Procedure 1. Contact the Histopathology Department, ensure that patient details and patient representative (usually a parent) details (including contact number) are supplied. 2. Request is received and action approved by the Pathologist involved with the case. 3. Specimens are not usually released until one month after the report has been verified. 4. Histopathology staff will then liaise with the patient's representative and undertake the following procedure. i. The specimen is washed in gentle running water for one hour (minimum). ii. The biopsy is blotted dry and sealed in a biodegradable plastic bag iii. The bag is labelled with "The Children's Hospital at Westmead", patient's name and the biopsy specimen laboratory accession number. iv. The specimen is placed in a small esky or similar container. v. A copy of the 10% formalin MSDS form is attached to the container. vi. The details of the specimen transfer from Histopathology to the family representative are recorded on the release form (Attachment 1), a copy is given to the parents and the original is attached to the specimen report. vii. Explanation is given to the parents regarding the possible hazardous nature of the specimen. That is: o If the specimen is for burial, ensure a deep hole. o Ensure that the specimen is kept away from children or pets. o Stress that the danger does not necessarily only lie with the possible infectious nature of the tissue but also the hazardous nature of the preservative. o Remind the parents to be familiar with the MSDS form and if concerned contact the laboratory (phone number included on the MSDS). The above information is included on the Release Form References: 1. Royal College of Pathologists (2001) "Transitional guidelines to facilitate changes in procedures for handling 'surplus' and archival material from human biological samples " http://www.rcpath.org/index.php?PageID=208 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, 27 November 2008 2:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest,Return of Body Parts for religious reasons You might want to check with local regulations concerning burial. Here, and at other facilities that I've been a part of, we receive these requests regularly, usually associated with amputated limbs, however, we make it a policy to release no "soft tissue" to a patient, but instead we ask that they make arrangements with a funeral home (since this is supposed to be for purposes of burial, eventually) and it is only to the funeral home that we will release the part. What happens to the part after that, is between the patient and their funeral home representative, which is much more familiar with regulations and practices for these situations. This is the only way to complete way protect the hospital from liability of having a body part "stored in the attic". Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services ------------------------------------------------------------------------ --------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From jnocito <@t> satx.rr.com Wed Nov 26 15:58:44 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Nov 26 15:58:49 2008 Subject: [Histonet] Ventana Benchmark XTs References: <2DFAEEFF192A9141ABACFF88BE613BF30BBC56@EXMBXC1.crha.bewell.ca> Message-ID: <40DFBD0D78644D8ABF5F5B26E40B4E0D@yourxhtr8hvc4p> oh yeah, many times. I had three at my last place. One was always going down for one reason or another. I wanted them to replace the machine, but they kept putting bandages on a bleeding wound. I went up to the regional maintenance manager and never got an answer. I guess the Lemon Law doesn't apply to Ventana. Of course, a lot of things don't apply to Ventana now, does it? JTT ----- Original Message ----- From: "Tracey Lenek" To: Cc: "Martin Trotter" ; "Joanna Bartczak-McKay" Sent: Wednesday, November 26, 2008 10:59 AM Subject: [Histonet] Ventana Benchmark XTs Hi, We have had on-going thermal slide pad issues with our XTs since they were installed in February. The slide tray assemblies have been replaced not once but twice and we are still having inconsistent results with the temp verifier slides. Has anyone experienced the same issues with this instrumentation? Thanks Tracey Lenek Tech III - Anatomic Pathology Calgary Laboratory Services 403-770-3588 ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RossS <@t> BaylorHealth.edu Wed Nov 26 16:00:50 2008 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Wed Nov 26 16:00:56 2008 Subject: [Histonet] RE: Histonet Digest, Return of Body Parts for religious reasons In-Reply-To: Message-ID: There are laws here in Texas that regulate all body parts as hazardous waste. If it isn't going to a funeral home the body part must go to a licensed hazardous waste handler. Our legal department has indicated they don't feel that under Texas law there is an exception for releasing body parts even with a subpoena. We had to have that discussion once. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Wednesday, November 26, 2008 3:50 PM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Histonet Digest, Return of Body Parts for religious reasons Amazing how different areas have different regs (or lack of) I thought I would attach our policy, seems to work and, drafted with the best intentions, hopefully prevents litigation and meets the needs of patients: BIOPSY SPECIMENS: RETURNED TO PATIENTS PROCEDURE (c) Background At present, very few patients request the return of resected organs or other samples. When patients are specifically informed of what happens to such specimens, it is likely that such requests will increase in frequency, especially in relation to larger samples including whole organs, the disposal or retention of which may have emotional implications. Laboratories should have established protocols to permit compliance with such requests while minimising any risk to the patient or others from infection or toxic chemicals. Procedure 1. Contact the Histopathology Department, ensure that patient details and patient representative (usually a parent) details (including contact number) are supplied. 2. Request is received and action approved by the Pathologist involved with the case. 3. Specimens are not usually released until one month after the report has been verified. 4. Histopathology staff will then liaise with the patient's representative and undertake the following procedure. i. The specimen is washed in gentle running water for one hour (minimum). ii. The biopsy is blotted dry and sealed in a biodegradable plastic bag iii. The bag is labelled with "The Children's Hospital at Westmead", patient's name and the biopsy specimen laboratory accession number. iv. The specimen is placed in a small esky or similar container. v. A copy of the 10% formalin MSDS form is attached to the container. vi. The details of the specimen transfer from Histopathology to the family representative are recorded on the release form (Attachment 1), a copy is given to the parents and the original is attached to the specimen report. vii. Explanation is given to the parents regarding the possible hazardous nature of the specimen. That is: o If the specimen is for burial, ensure a deep hole. o Ensure that the specimen is kept away from children or pets. o Stress that the danger does not necessarily only lie with the possible infectious nature of the tissue but also the hazardous nature of the preservative. o Remind the parents to be familiar with the MSDS form and if concerned contact the laboratory (phone number included on the MSDS). The above information is included on the Release Form References: 1. Royal College of Pathologists (2001) "Transitional guidelines to facilitate changes in procedures for handling 'surplus' and archival material from human biological samples " http://www.rcpath.org/index.php?PageID=208 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, 27 November 2008 2:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest,Return of Body Parts for religious reasons You might want to check with local regulations concerning burial. Here, and at other facilities that I've been a part of, we receive these requests regularly, usually associated with amputated limbs, however, we make it a policy to release no "soft tissue" to a patient, but instead we ask that they make arrangements with a funeral home (since this is supposed to be for purposes of burial, eventually) and it is only to the funeral home that we will release the part. What happens to the part after that, is between the patient and their funeral home representative, which is much more familiar with regulations and practices for these situations. This is the only way to complete way protect the hospital from liability of having a body part "stored in the attic". Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax The premise I've been given is so that, upon death, the patient may be stored with her body parts. My facility has concerns about providing it to her in formalin (for obvious reasons) or alcohol. The patient admits this is a family practice with momma's appendix already being stored in the attic. It can get a bit toasty warm here in the South so attic storage of a specimen in alcohol may not be prudent and I can't be absolutely certain it wouldn't burn the house down, another potential liability for my institution. I'm tempted to give it to her in food grade vinegar, to avoid the potential liabilities from using anything that could be considered hazardous. Assuming that returning her gall bladder is a given, what do you think of using vinegar for this purpose? Vinnie Della Speranza Manager for Anatomic Pathology Services ------------------------------------------------------------------------ --------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From mendozan <@t> xoma.com Wed Nov 26 16:05:44 2008 From: mendozan <@t> xoma.com (Nerissa Mendoza) Date: Wed Nov 26 16:05:04 2008 Subject: [Histonet] RE: therapeutic antibody detection in mouse--Histonet Digest, Vol 60, Issue 33 In-Reply-To: <20081120155133.006191834F7@barracuda1.xoma.com> Message-ID: <9726B898F3BDF84181BF5BC99508274A01945142@CYPRESS4.xoma.com> Sam, Isn't Herceptin a humanized mAb? I believe you need to detect it with an anti-human antibody (rather than mouse)? Nerissa Mendoza Senior Research Associate I Preclinical Research & Development XOMA (US) LLC 2910 Seventh Street Berkeley, CA 94710 ------------------------------ Message: 3 Date: Wed, 19 Nov 2008 14:22:57 -0500 From: "Perry, Samuel" Subject: [Histonet] therapeutic antibody detection in mouse xenografts? To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi All, I am wondering if anyone has experience showing therapeutic monoclonal antibody binding in mouse xenografts (human tumor cells injected subcutaneously in SCID mice and then dose treated with therapeutic antibody injected intraperitoneally). For instance, can trastuzamab (Herceptin) binding be seen in a breast tumor xenograft treated with Herceptin? I have tried using Dako's goat-anti mouse dextran HRP conjugated secondary antibody developed with DAB. I also tried using an Abcam goat-anti mouse antibody conjugated with biotin, then adding Vectors elite ABC, then developing with DAB. Both times I used FFPE tissue. In both techniques, results showed minimal to staining and showed no difference between mice treated with antibody and control mice treated. I would appreciate if anyone knew of published papers or have experience using IHC to show therapeutic antibody bound to tumor cell in mouse xenografts. Thanks -Sam Research Technician Dana Farber Cancer Institute Boston MA. The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. -- The information contained in this email message may contain confidential or legally privileged information and is intended solely for the use of the named recipient(s). No confidentiality or privilege is waived or lost by any transmission error. If the reader of this message is not the intended recipient, please immediately delete the e-mail and all copies of it from your system, destroy any hard copies of it and notify the sender either by telephone or return e-mail. Any direct or indirect use, disclosure, distribution, printing, or copying of any part of this message is prohibited. Any views expressed in this message are those of the individual sender, except where the message states otherwise and the sender is authorized to state them to be the views of XOMA. From pieronelva01 <@t> bigpond.com Wed Nov 26 16:17:36 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Wed Nov 26 16:17:42 2008 Subject: [Histonet] Ventana Benchmark XTs References: <2DFAEEFF192A9141ABACFF88BE613BF30BBC56@EXMBXC1.crha.bewell.ca> Message-ID: Hi Tracey We had exactly that problem with inconsistent slide temps and failure to heat on random pads. Our local service technician said there was a manufacturing problem with all plates made during a certain time and that they all needed to be replaced worldwide. We had our pad tray replaced about a month ago and not a problem since. Perhaps you can check if your replacement tray was from the "new" batch. Regards Piero Nelva ----- Original Message ----- From: "Tracey Lenek" To: Cc: "Martin Trotter" ; "Joanna Bartczak-McKay" Sent: Thursday, November 27, 2008 3:59 AM Subject: [Histonet] Ventana Benchmark XTs Hi, We have had on-going thermal slide pad issues with our XTs since they were installed in February. The slide tray assemblies have been replaced not once but twice and we are still having inconsistent results with the temp verifier slides. Has anyone experienced the same issues with this instrumentation? Thanks Tracey Lenek Tech III - Anatomic Pathology Calgary Laboratory Services 403-770-3588 ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.9.10/1812 - Release Date: 11/25/2008 7:53 PM From Lynn.Burton <@t> Illinois.gov Wed Nov 26 16:29:39 2008 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Wed Nov 26 16:31:08 2008 Subject: [Histonet] Ventana Benchmark XTs References: <2DFAEEFF192A9141ABACFF88BE613BF30BBC56@EXMBXC1.crha.bewell.ca> Message-ID: We have had some trouble but they are planning to replace them. We received a letter that they had changed vendors for parts and found they need to switch back. They did replace one section earlier when it stopped working completely. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tracey Lenek Sent: Wed 11/26/2008 10:59 AM To: histonet@lists.utsouthwestern.edu Cc: Martin Trotter; Joanna Bartczak-McKay Subject: [Histonet] Ventana Benchmark XTs Hi, We have had on-going thermal slide pad issues with our XTs since they were installed in February. The slide tray assemblies have been replaced not once but twice and we are still having inconsistent results with the temp verifier slides. Has anyone experienced the same issues with this instrumentation? Thanks Tracey Lenek Tech III - Anatomic Pathology Calgary Laboratory Services 403-770-3588 ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Thu Nov 27 13:16:23 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Nov 27 13:16:31 2008 Subject: [Histonet] Re: bone saws Message-ID: Stephen Peters, a pathologist in Hackensack NJ, describes for us a bone saw he's invented. His Web site is distinctly worth looking at, the whole thing, not just the saw. http://pathologyinnovations.com/bone_vise.htm Some other possibilities for sawing bone: Many of the small pathology services I work for have no way of sawing bone. (It's amazing how many pathologists there are who are so poorly trained that they think you decalcify a femoral head by tossing the whole thing in formalin for a month.) In that circumstance, I head for the nearest hardware store and buy a five dollar hacksaw which I leave behind at the end of the week. The Civil War vintage Satterlee amputation saw is still available, and is a serviceable hand saw that doesn't go dull quickly. (I've seen them, complete with chrome plating, at Civil War re-enactments.) This is the tool I most commonly use. One of the standard vendors offers a simple device for slabbing a femoral head, the SawBones, absurdly overpriced at $500, not something a hospital would be likely to buy for a mere pathologist. Several years ago I attended a continuing medical education program where the lecturer recommended a scroll saw, a large table saw that's about impossible to injure yourself with. At the time they cost about $100 for Made in China, otherwise $200. The disadvantage, in a cramped pathology lab, is its large footprint. You can look at these things at your local Home Depot. There's no way to cut a femoral head safely with an oscillating autopsy saw (Stryker saw), though this is probably the most common way to cut bone. I think femoral heads removed for fracture (not for osteoarthritis) really do need to be examined microscopically, because of the occasional pathologic fracture (fracture through metastatic cancer in the bone). I've seen several of these, not all with a previous cancer diagnosis. (But I see no reason to examine knee replacement material microscopically, if you know how to do the gross description properly - which admittedly most pathologists don't.) (I have no connection with any of the businesses I've mentioned.) Bob Richmond Samurai Pathologist Knoxville TN From c.m.vanderloos <@t> amc.uva.nl Fri Nov 28 03:02:21 2008 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Nov 28 03:02:32 2008 Subject: [Histonet] problem with NSH website??? Message-ID: Dear All,Desperately I tried to submit my abstracts for the NSH convention. Using the links for submission in the email we got from Aubrey Wanner/Kim Simmons early November, the browser goes to Isovera.com a domain for sale in Germany, provided by Sedo Domain Parking. Obviously something that has nothing to do with NSH. The same problems occurs at the NSH website. You can go to the NSH website as normal, but clicking on 'meetings/event' and selecting 'symposium/convention' one is re-directed to that same crap. What is going on here??? Does this problem also occurs with others???Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands From je2 <@t> sanger.ac.uk Fri Nov 28 03:24:36 2008 From: je2 <@t> sanger.ac.uk (Jeanne Estabel) Date: Fri Nov 28 03:24:44 2008 Subject: [Histonet] Anti-beta galactosidase antibody for FFPE tissues Message-ID: <8DAC27FBBF33764B9F3DCF0A3E6B09620287F74B@exchsrv2.internal.sanger.ac.uk> Hi, We want to use anti-betagalactosidase antibodies on FFPE mouse sections for a high throuput project. We have already try a lot of different ones without reliable and constant staining. Any suggestion of a good one? Please could you share your experience with these antibodies. Regards Jeanne Estabel, PhD MGP Histologist, Team 109 Wellcome Trust/Sanger Institute Hinxton CB10 1SA Tel: 01223 495338 -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a compa ny registered in England with number 2742969, whose registered office is 2 15 Euston Road, London, NW1 2BE. From mucram11 <@t> comcast.net Fri Nov 28 07:31:13 2008 From: mucram11 <@t> comcast.net (Pamela Marcum) Date: Fri Nov 28 07:31:18 2008 Subject: [Histonet] problem with NSH website??? In-Reply-To: Message-ID: <1936081588.1340981227879073790.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> I am not sure what is going on Chris.? I just tried it and I got the same result you did going to Isovers etc.? Obviously something is very wrong and they need to correct it.? I will forward you e-mail and my answer to them so they know it is not just a problem for Europe.? I assume they monitor HistoNet - I could be wrong. Pam Marcum ----- Original Message ----- From: "C.M. van der Loos" To: "histonet netserver" Sent: Friday, November 28, 2008 4:02:21 AM GMT -05:00 US/Canada Eastern Subject: [Histonet] problem with NSH website??? Dear All,Desperately I tried to submit my abstracts for the NSH convention. Using the links for submission in the email we got from Aubrey Wanner/Kim Simmons early November, the browser goes to Isovera.com a domain for sale in Germany, provided by Sedo Domain Parking. Obviously something that has nothing to do with NSH. The same problems occurs at the NSH website. You can go to the NSH website as normal, but clicking on 'meetings/event' and selecting 'symposium/convention' one is re-directed to that same crap. What is going on here??? Does this problem also occurs with others???Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Fri Nov 28 07:48:52 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Nov 28 07:49:42 2008 Subject: [Histonet] Sakura tape problem References: <5D64396A0D4A5346BEBC759022AAEAA5169B32@ITSSSXM01V6.one.ads.che.org> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F284B@fhosxchmb006.ADVENTISTCORP.NET> Be careful! The Acetone dissolves the tape! Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org 407-646-7559 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Fri 11/21/2008 2:36 PM To: Jackie M O'Connor; Cheryl Crowder Cc: histonet-bounces@lists.utsouthwestern.edu; Histonet Subject: RE: [Histonet] Sakura tape problem How do you do this with the tissue attached to the tape? Just curious... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, November 21, 2008 2:32 PM To: Cheryl Crowder Cc: histonet-bounces@lists.utsouthwestern.edu; Histonet Subject: Re: [Histonet] Sakura tape problem I would remove the tape with acetone and clear in xylene, then recoverslip with conventional coverslips. I've seen problems with refractivity with the tape, anyway. Happy Turkey Day. Jackie O' "Cheryl Crowder" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/21/2008 01:20 PM To "Histonet" cc Subject [Histonet] Sakura tape problem Hi - I have just been given a box (100 slides) cover slipped with Sakura tape. All the tapes have been loosened from the slides with the tissue attacked to it. The tape has also curled (arched). What is the best method for reattaching the tapes to the slides so they can be viewed again. Or can it? The pathologists are really upset over these slides as they are for continuing education and cannot be replaced. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From barbara.schormair <@t> helmholtz-muenchen.de Fri Nov 28 08:35:17 2008 From: barbara.schormair <@t> helmholtz-muenchen.de (Barbara Schormair) Date: Fri Nov 28 08:35:26 2008 Subject: [Histonet] Thanks for help on "desperately seeking help for cryosectioning" and further question Message-ID: <493001A5.8000605@helmholtz-muenchen.de> Dear All, thank you so much for your suggestions on improving my cryosectioning results. I tried several of your suggestions, however, the quality of my sections did'nt improve so much. I get perfect sections as long as I only cut the OCT embedding media. As soon as I reach embryonal tissue, only the tissue starts to tear up. In some of the embryos I loose almost all tissue. In my opinion this can only be caused by the fixation of the mouse embryos. Here is our protocol: 1. Fixation in 4% PFA overnight at 4?C 2. 30% sucrose solution at 4?C till embryo sinks to bottom of the tube 3. transfer to 1:1 mix of 30% sucrose solution and OCT for 4h at 4?C 4. transfer to 100% OCT and freeze quickly in isopentane (cooled down with dry ice for approx. 30min before use). 5. store at -80?C Is there something wrong? Should I follow a different protocol`? How long can you store OCT-embedded tissue? Are 6 months too long? Thanks so much for your help and best regards, Barbara -- Dipl. Biol. Barbara Schormair PhD student Institute of Human Genetics Tel.: 0049-89-3187-4097 Fax: 0049-89-3187-3474 e-Mail: barbara.schormair@helmholtz-muenchen.de Helmholtz Zentrum M?nchen German Research Center for Environmental Health (GmbH) Ingolstaedter Landstra?e 1 D-85764 Neuherberg Germany Chairman of Supervisory Board: MinDir Dr. Peter Lange Board of Directors: Prof. Dr. G?nther Wess and Dr. Nikolaus Blum Register of Societies: Amtsgericht M?nchen HRB 6466 From Barry.R.Rittman <@t> uth.tmc.edu Fri Nov 28 10:18:38 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Nov 28 10:18:42 2008 Subject: [Histonet] Thanks for help on "desperately seeking help for cryosectioning" and further question References: <493001A5.8000605@helmholtz-muenchen.de> Message-ID: Barbara Happy thanksgiving. 1. would recommend that you soak the tissues at step 4 in OCT for some time say half an hour to an hour to allow better penetration of the OCT throughout the tissue. Sounds to me that the OCT has only partly replaced the OCT/sucrose mixture. 2. I would recommend that if you are storing at -80C that you includes some ice cubes in the small box or bag that you use to store reduce the risk of dehydrating the block especially on long term storage. Good luck. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Barbara Schormair Sent: Fri 11/28/2008 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thanks for help on "desperately seeking help for cryosectioning" and further question Dear All, thank you so much for your suggestions on improving my cryosectioning results. I tried several of your suggestions, however, the quality of my sections did'nt improve so much. I get perfect sections as long as I only cut the OCT embedding media. As soon as I reach embryonal tissue, only the tissue starts to tear up. In some of the embryos I loose almost all tissue. In my opinion this can only be caused by the fixation of the mouse embryos. Here is our protocol: 1. Fixation in 4% PFA overnight at 4?C 2. 30% sucrose solution at 4?C till embryo sinks to bottom of the tube 3. transfer to 1:1 mix of 30% sucrose solution and OCT for 4h at 4?C 4. transfer to 100% OCT and freeze quickly in isopentane (cooled down with dry ice for approx. 30min before use). 5. store at -80?C Is there something wrong? Should I follow a different protocol`? How long can you store OCT-embedded tissue? Are 6 months too long? Thanks so much for your help and best regards, Barbara -- Dipl. Biol. Barbara Schormair PhD student Institute of Human Genetics Tel.: 0049-89-3187-4097 Fax: 0049-89-3187-3474 e-Mail: barbara.schormair@helmholtz-muenchen.de Helmholtz Zentrum M?nchen German Research Center for Environmental Health (GmbH) Ingolstaedter Landstra?e 1 D-85764 Neuherberg Germany Chairman of Supervisory Board: MinDir Dr. Peter Lange Board of Directors: Prof. Dr. G?nther Wess and Dr. Nikolaus Blum Register of Societies: Amtsgericht M?nchen HRB 6466 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotechkb <@t> gmail.com Fri Nov 28 10:33:24 2008 From: histotechkb <@t> gmail.com (Kristen Yaros) Date: Fri Nov 28 10:33:29 2008 Subject: [Histonet] problem with NSH website??? In-Reply-To: <1936081588.1340981227879073790.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <1936081588.1340981227879073790.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <667c97ab0811280833k7d652673w4188780c9dd0df8c@mail.gmail.com> It was happening to me last night as well. I wonder if a server or something is down... somewhere. Kristen On Fri, Nov 28, 2008 at 8:31 AM, Pamela Marcum wrote: > > > I am not sure what is going on Chris. I just tried it and I got the same > result you did going to Isovers etc. Obviously something is very wrong and > they need to correct it. I will forward you e-mail and my answer to them so > they know it is not just a problem for Europe. I assume they monitor > HistoNet - I could be wrong. > > > > Pam Marcum > > > ----- Original Message ----- > From: "C.M. van der Loos" > To: "histonet netserver" > Sent: Friday, November 28, 2008 4:02:21 AM GMT -05:00 US/Canada Eastern > Subject: [Histonet] problem with NSH website??? > > Dear All,Desperately I tried to submit my abstracts for the NSH convention. > Using the links for submission in the email we got from Aubrey Wanner/Kim > Simmons early November, the browser goes to Isovera.com a domain for sale in > Germany, provided by Sedo Domain Parking. Obviously something that has > nothing to do with NSH. The same problems occurs at the NSH website. You can > go to the NSH website as normal, but clicking on 'meetings/event' and > selecting 'symposium/convention' one is re-directed to that same crap. What > is going on here??? Does this problem also occurs with others???Chris van > der Loos, PhD > Dept. of Pathology > Academic Medical Center M2-230 > Meibergdreef 9 > NL-1105 AZ Amsterdam > The Netherlands > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Kristen Yaros, HT (ASCP)CM Histotechnology Society of Delaware Correspondence Secretary histotechkb@gmail.com From bakevictoria <@t> gmail.com Fri Nov 28 11:19:16 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Nov 28 11:19:21 2008 Subject: [Histonet] problem with NSH website??? In-Reply-To: <667c97ab0811280833k7d652673w4188780c9dd0df8c@mail.gmail.com> References: <1936081588.1340981227879073790.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> <667c97ab0811280833k7d652673w4188780c9dd0df8c@mail.gmail.com> Message-ID: <4f016b690811280919i67ac3214i215f94eca0f741f2@mail.gmail.com> The office is closed for the holiday, as I have tried calling them. I also tried to use the website over the holiday and had the same issues that Chris van der Loos had. I have faith - all will be restored by Monday afternoon! ;-) Hope everyone had a happy and festive Thanksgiving! Vikki On Fri, Nov 28, 2008 at 11:33 AM, Kristen Yaros wrote: > It was happening to me last night as well. I wonder if a server or something > is down... somewhere. > > Kristen > > On Fri, Nov 28, 2008 at 8:31 AM, Pamela Marcum wrote: > >> >> >> I am not sure what is going on Chris. I just tried it and I got the same >> result you did going to Isovers etc. Obviously something is very wrong and >> they need to correct it. I will forward you e-mail and my answer to them so >> they know it is not just a problem for Europe. I assume they monitor >> HistoNet - I could be wrong. >> >> >> >> Pam Marcum >> >> >> ----- Original Message ----- >> From: "C.M. van der Loos" >> To: "histonet netserver" >> Sent: Friday, November 28, 2008 4:02:21 AM GMT -05:00 US/Canada Eastern >> Subject: [Histonet] problem with NSH website??? >> >> Dear All,Desperately I tried to submit my abstracts for the NSH convention. >> Using the links for submission in the email we got from Aubrey Wanner/Kim >> Simmons early November, the browser goes to Isovera.com a domain for sale in >> Germany, provided by Sedo Domain Parking. Obviously something that has >> nothing to do with NSH. The same problems occurs at the NSH website. You can >> go to the NSH website as normal, but clicking on 'meetings/event' and >> selecting 'symposium/convention' one is re-directed to that same crap. What >> is going on here??? Does this problem also occurs with others???Chris van >> der Loos, PhD >> Dept. of Pathology >> Academic Medical Center M2-230 >> Meibergdreef 9 >> NL-1105 AZ Amsterdam >> The Netherlands >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Kristen Yaros, HT (ASCP)CM > Histotechnology Society of Delaware > Correspondence Secretary > histotechkb@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lenaspencer <@t> insightbb.com Fri Nov 28 11:35:37 2008 From: lenaspencer <@t> insightbb.com (Lena Spencer) Date: Fri Nov 28 11:36:30 2008 Subject: [Histonet] Help with protocols Message-ID: <001801c9517f$b9838330$2c8a8990$@com> Hi All: I have been working up the PMS2 and MLH2 mismatched repair genes and I am not satisfied with my results. I am using the Cell Marque antibodies and the Ventana XT using CC1, mild, standard and I have tried the antibodies with block and amplification, and antibody times from 32 min- 1 hour and the stain is not satisfactory. Anyone who has these antibodies in their lab and would like to share your protocols, I would be grateful for your help. The MSH2 and MSH6 are working great. Lena Spencer From histonetalias <@t> gmail.com Fri Nov 28 13:51:50 2008 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Fri Nov 28 13:51:55 2008 Subject: [Histonet] Ventana Benchmark XTs In-Reply-To: References: <2DFAEEFF192A9141ABACFF88BE613BF30BBC56@EXMBXC1.crha.bewell.ca> Message-ID: <4b6c85510811281151w4aec5437ybf85920655ab9def@mail.gmail.com> They are switching to a more reliable manufacturer. They are being proactive and switching them before they fail down the road. My XT has been a workhorse but we know certain peoples opinions about the company on here. I will take it any day over the rest. On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn wrote: > We have had some trouble but they are planning to replace them. We received > a letter that they had changed vendors for parts and found they need to > switch back. They did replace one section earlier when it stopped working > completely. > > Lynn Burton > Lab Assoc. I > Animal Disease Lab > Galesburg, Il 61401 > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tracey Lenek > Sent: Wed 11/26/2008 10:59 AM > To: histonet@lists.utsouthwestern.edu > Cc: Martin Trotter; Joanna Bartczak-McKay > Subject: [Histonet] Ventana Benchmark XTs > > > > Hi, > > We have had on-going thermal slide pad issues with our XTs since they were > installed > in February. The slide tray assemblies have been replaced not once but > twice and we are still having inconsistent > results with the temp verifier slides. Has anyone experienced the same > issues with this > instrumentation? > > Thanks > Tracey Lenek > Tech III - Anatomic Pathology > Calgary Laboratory Services > 403-770-3588 > > ________________________________ > This message and any attached documents are only for the use of the > intended recipient(s), are confidential and may contain privileged > information. Any unauthorized review, use, retransmission, or other > disclosure is strictly prohibited. If you have received this message in > error, please notify the sender immediately, and then delete the original > message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- The Unknown HT(ASCP) From histonetalias <@t> gmail.com Fri Nov 28 13:58:48 2008 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Fri Nov 28 13:58:51 2008 Subject: [Histonet] Help with protocols In-Reply-To: <001801c9517f$b9838330$2c8a8990$@com> References: <001801c9517f$b9838330$2c8a8990$@com> Message-ID: <4b6c85510811281158s7bf460bdkfc3da1c499d31c15@mail.gmail.com> If the CC1 standard 32 with amp, 42 degrees does not work then try using protease 1 4-6 minutes on it. On Fri, Nov 28, 2008 at 12:35 PM, Lena Spencer wrote: > Hi All: > > I have been working up the PMS2 and MLH2 mismatched repair genes and I am > not satisfied with my results. I am using the Cell Marque antibodies and > the Ventana XT using CC1, mild, standard and I have tried the antibodies > with block and amplification, and antibody times from 32 min- 1 hour and > the > stain is not satisfactory. Anyone who has these antibodies in their lab > and > would like to share your protocols, I would be grateful for your help. > The MSH2 and MSH6 are working great. > > Lena Spencer > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- The Unknown HT(ASCP) From marktarango <@t> gmail.com Fri Nov 28 14:10:31 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Nov 28 14:10:37 2008 Subject: [Histonet] Ventana Benchmark XTs In-Reply-To: <4b6c85510811281151w4aec5437ybf85920655ab9def@mail.gmail.com> References: <2DFAEEFF192A9141ABACFF88BE613BF30BBC56@EXMBXC1.crha.bewell.ca> <4b6c85510811281151w4aec5437ybf85920655ab9def@mail.gmail.com> Message-ID: <5b6eb13e0811281210j7fd2f0d5w1c3283bd9bdead11@mail.gmail.com> I don't know how much the opinion of "The Unknown HT(ASCP)" counts. You aren't with Ventana I hope. Mark On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias wrote: > They are switching to a more reliable manufacturer. They are being > proactive > and switching them before they fail down the road. My XT has been a > workhorse but we know certain peoples opinions about the company on here. I > will take it any day over the rest. > > On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn >wrote: > > > We have had some trouble but they are planning to replace them. We > received > > a letter that they had changed vendors for parts and found they need to > > switch back. They did replace one section earlier when it stopped working > > completely. > > > > Lynn Burton > > Lab Assoc. I > > Animal Disease Lab > > Galesburg, Il 61401 > > > > ________________________________ > > > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tracey > Lenek > > Sent: Wed 11/26/2008 10:59 AM > > To: histonet@lists.utsouthwestern.edu > > Cc: Martin Trotter; Joanna Bartczak-McKay > > Subject: [Histonet] Ventana Benchmark XTs > > > > > > > > Hi, > > > > We have had on-going thermal slide pad issues with our XTs since they > were > > installed > > in February. The slide tray assemblies have been replaced not once but > > twice and we are still having inconsistent > > results with the temp verifier slides. Has anyone experienced the same > > issues with this > > instrumentation? > > > > Thanks > > Tracey Lenek > > Tech III - Anatomic Pathology > > Calgary Laboratory Services > > 403-770-3588 > > > > ________________________________ > > This message and any attached documents are only for the use of the > > intended recipient(s), are confidential and may contain privileged > > information. Any unauthorized review, use, retransmission, or other > > disclosure is strictly prohibited. If you have received this message in > > error, please notify the sender immediately, and then delete the original > > message. Thank you. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > The Unknown HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jkiernan <@t> uwo.ca Fri Nov 28 15:12:40 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Nov 28 15:12:45 2008 Subject: [Histonet] Hematoxylin staining and Calcification Message-ID: Yes. Lillie RD & Fullmer HM 1976. Histopathologic Technic an York: McG quarter of the way do page. 
  
John Kiernan
 Anatomy, UWO
 London, Canada
Original Message --- AGrobe2555@aol.com
> Date: 25, 2008 15:06
> Subject: [Hi Hematoxylin staining and Calcification
> To: h istonet@lists.utsouthwestern.edu
>
> there anything publish
> > > calcification (primaril hematoxylin)?
> > Thanks,
> > Albert 
> >
> & Tissue Institute
> site has it all. > social networks, you love. Try the new AOL.
> today!(http://pr.atwo la.com/promoclk/100000075x1212962939x1200825291/aol?redir=http=3 A/ %26icid= aolcom40vanity%26ncid=emlcntaolcom00000001)
> > _______________________ 5F _______________________ 3C > Hi > http:// lists.utsouthwestern.edu/mailman/listinfo/histonet
> From jnocito <@t> satx.rr.com Fri Nov 28 17:19:02 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Nov 28 17:18:56 2008 Subject: [Histonet] Ventana Benchmark XTs References: <2DFAEEFF192A9141ABACFF88BE613BF30BBC56@EXMBXC1.crha.bewell.ca><4b6c85510811281151w4aec5437ybf85920655ab9def@mail.gmail.com> <5b6eb13e0811281210j7fd2f0d5w1c3283bd9bdead11@mail.gmail.com> Message-ID: <620DA3ADD5764A27B91126F3FFC781D2@yourxhtr8hvc4p> no, they usually contact the CEO's of the labs to get their employees to stop ragging on the company. Then again, you never know who is lurking in the winds. JTT ----- Original Message ----- From: "Mark Tarango" To: "Histonet Alias" Cc: ; "Martin Trotter" ; "Tracey Lenek" ; "Burton,Lynn" ; "Joanna Bartczak-McKay" Sent: Friday, November 28, 2008 2:10 PM Subject: Re: [Histonet] Ventana Benchmark XTs >I don't know how much the opinion of "The Unknown HT(ASCP)" counts. You > aren't with Ventana I hope. > > Mark > > On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias > wrote: > >> They are switching to a more reliable manufacturer. They are being >> proactive >> and switching them before they fail down the road. My XT has been a >> workhorse but we know certain peoples opinions about the company on here. >> I >> will take it any day over the rest. >> >> On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn > >wrote: >> >> > We have had some trouble but they are planning to replace them. We >> received >> > a letter that they had changed vendors for parts and found they need to >> > switch back. They did replace one section earlier when it stopped >> > working >> > completely. >> > >> > Lynn Burton >> > Lab Assoc. I >> > Animal Disease Lab >> > Galesburg, Il 61401 >> > >> > ________________________________ >> > >> > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tracey >> Lenek >> > Sent: Wed 11/26/2008 10:59 AM >> > To: histonet@lists.utsouthwestern.edu >> > Cc: Martin Trotter; Joanna Bartczak-McKay >> > Subject: [Histonet] Ventana Benchmark XTs >> > >> > >> > >> > Hi, >> > >> > We have had on-going thermal slide pad issues with our XTs since they >> were >> > installed >> > in February. The slide tray assemblies have been replaced not once but >> > twice and we are still having inconsistent >> > results with the temp verifier slides. Has anyone experienced the same >> > issues with this >> > instrumentation? >> > >> > Thanks >> > Tracey Lenek >> > Tech III - Anatomic Pathology >> > Calgary Laboratory Services >> > 403-770-3588 >> > >> > ________________________________ >> > This message and any attached documents are only for the use of the >> > intended recipient(s), are confidential and may contain privileged >> > information. Any unauthorized review, use, retransmission, or other >> > disclosure is strictly prohibited. If you have received this message in >> > error, please notify the sender immediately, and then delete the >> > original >> > message. Thank you. >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> > >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> >> >> >> -- >> The Unknown HT(ASCP) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccrowder <@t> vetmed.lsu.edu Fri Nov 28 19:14:00 2008 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Fri Nov 28 19:16:37 2008 Subject: [Histonet] Sakura tape problem In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47807F284B@fhosxchmb006.ADVENTISTCORP.NET> References: <5D64396A0D4A5346BEBC759022AAEAA5169B32@ITSSSXM01V6.one.ads.che.org> <5F31F38C96781A4FBE3196EBC22D47807F284B@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: Thanks for the tip. All I'd need to do is lose the tissue too. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 -----Original Message----- From: "Bonner, Janet" To: "Weems, Joyce" , "Jackie M O'Connor" , "Cheryl Crowder" Cc: histonet-bounces@lists.utsouthwestern.edu, "Histonet" Date: Fri, 28 Nov 2008 08:48:52 -0500 Subject: RE: [Histonet] Sakura tape problem Be careful! The Acetone dissolves the tape! Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org [mailto:janet.bonner@FLHOSP.org] 407-646-7559 From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Fri 11/21/2008 2:36 PM To: Jackie M O'Connor; Cheryl Crowder Cc: histonet-bounces@lists.utsouthwestern.edu; Histonet Subject: RE: [Histonet] Sakura tape problem How do you do this with the tissue attached to the tape? Just curious... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]] On Behalf Of Jackie M O'Connor Sent: Friday, November 21, 2008 2:32 PM To: Cheryl Crowder Cc: histonet-bounces@lists.utsouthwestern.edu; Histonet Subject: Re: [Histonet] Sakura tape problem I would remove the tape with acetone and clear in xylene, then recoverslip with conventional coverslips. I've seen problems with refractivity with the tape, anyway. Happy Turkey Day. Jackie O' "Cheryl Crowder" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/21/2008 01:20 PM To "Histonet" cc Subject [Histonet] Sakura tape problem Hi - I have just been given a box (100 slides) cover slipped with Sakura tape. All the tapes have been loosened from the slides with the tissue attacked to it. The tape has also curled (arched). What is the best method for reattaching the tapes to the slides so they can be viewed again. Or can it? The pathologists are really upset over these slides as they are for continuing education and cannot be replaced. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [http://lists.utsouthwestern.edu/mailman/listinfo/histonet] _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [http://lists.utsouthwestern.edu/mailman/listinfo/histonet] Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [http://lists.utsouthwestern.edu/mailman/listinfo/histonet] ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From marktarango <@t> gmail.com Fri Nov 28 19:50:32 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Nov 28 19:50:38 2008 Subject: [Histonet] Ventana Benchmark XTs In-Reply-To: <620DA3ADD5764A27B91126F3FFC781D2@yourxhtr8hvc4p> References: <2DFAEEFF192A9141ABACFF88BE613BF30BBC56@EXMBXC1.crha.bewell.ca> <4b6c85510811281151w4aec5437ybf85920655ab9def@mail.gmail.com> <5b6eb13e0811281210j7fd2f0d5w1c3283bd9bdead11@mail.gmail.com> <620DA3ADD5764A27B91126F3FFC781D2@yourxhtr8hvc4p> Message-ID: <5b6eb13e0811281750t591e3fc7ja9a63d74c91b4382@mail.gmail.com> Yup, been through that before. I made sure that we switched to BioCare's reagents on a dako-like stainer after that. I would think that'd be the only reason to hide your identity (fear of Ventana sending the hounds after you). On Fri, Nov 28, 2008 at 3:19 PM, Joe Nocito wrote: > no, they usually contact the CEO's of the labs to get their employees to > stop ragging on the company. Then again, you never know who is lurking in > the winds. > > JTT > ----- Original Message ----- From: "Mark Tarango" > To: "Histonet Alias" > Cc: ; "Martin Trotter" < > Martin.Trotter@cls.ab.ca>; "Tracey Lenek" ; > "Burton,Lynn" ; "Joanna Bartczak-McKay" < > Joanna.Bartczak-McKay@cls.ab.ca> > Sent: Friday, November 28, 2008 2:10 PM > Subject: Re: [Histonet] Ventana Benchmark XTs > > > I don't know how much the opinion of "The Unknown HT(ASCP)" counts. You >> aren't with Ventana I hope. >> >> Mark >> >> On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias > >wrote: >> >> They are switching to a more reliable manufacturer. They are being >>> proactive >>> and switching them before they fail down the road. My XT has been a >>> workhorse but we know certain peoples opinions about the company on here. >>> I >>> will take it any day over the rest. >>> >>> On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn >> >wrote: >>> >>> > We have had some trouble but they are planning to replace them. We >>> received >>> > a letter that they had changed vendors for parts and found they need to >>> > switch back. They did replace one section earlier when it stopped > >>> working >>> > completely. >>> > >>> > Lynn Burton >>> > Lab Assoc. I >>> > Animal Disease Lab >>> > Galesburg, Il 61401 >>> > >>> > ________________________________ >>> > >>> > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tracey >>> Lenek >>> > Sent: Wed 11/26/2008 10:59 AM >>> > To: histonet@lists.utsouthwestern.edu >>> > Cc: Martin Trotter; Joanna Bartczak-McKay >>> > Subject: [Histonet] Ventana Benchmark XTs >>> > >>> > >>> > >>> > Hi, >>> > >>> > We have had on-going thermal slide pad issues with our XTs since they >>> were >>> > installed >>> > in February. The slide tray assemblies have been replaced not once but >>> > twice and we are still having inconsistent >>> > results with the temp verifier slides. Has anyone experienced the same >>> > issues with this >>> > instrumentation? >>> > >>> > Thanks >>> > Tracey Lenek >>> > Tech III - Anatomic Pathology >>> > Calgary Laboratory Services >>> > 403-770-3588 >>> > >>> > ________________________________ >>> > This message and any attached documents are only for the use of the >>> > intended recipient(s), are confidential and may contain privileged >>> > information. Any unauthorized review, use, retransmission, or other >>> > disclosure is strictly prohibited. If you have received this message in >>> > error, please notify the sender immediately, and then delete the > >>> original >>> > message. Thank you. >>> > _______________________________________________ >>> > Histonet mailing list >>> > Histonet@lists.utsouthwestern.edu >>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > >>> > >>> > _______________________________________________ >>> > Histonet mailing list >>> > Histonet@lists.utsouthwestern.edu >>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > >>> >>> >>> >>> -- >>> The Unknown HT(ASCP) >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From stevenhacker <@t> verizon.net Fri Nov 28 22:13:59 2008 From: stevenhacker <@t> verizon.net (Steven Hacker) Date: Fri Nov 28 22:14:15 2008 Subject: [Histonet] Ventana Benchmark XTs Message-ID: <1589726357.931151227932039574.JavaMail.root@vms246.mailsrvcs.net> The Hound of the Baskerville? Nov 28, 2008 08:51:46 PM, ma Yup, been thr BioCare's reagents on be the only reason hounds after you). On Fri, Nov 28, 2008 at 3:19 PM, Joe Nocito wrote > no, they usually contact the CEO's of the labs to get their e mployees to > stop ragging on the company. Then again, you never know lurking in > the winds. > > JTT > ----- Ori > To > Cc: ; "Martin Trotter" > Martin.Trotter@cls.ab.ca>; > "Burton,Lynn" ; "Joanna Bartczak-McKay" > Joanna.Bartczak-McKay@cls. > Sent: Friday, November 28, 2008 2:10 PM > Subject: > > > I don't know h counts. You >> aren' >> >> Mark >> >> >> >wrote: >> >> They are switching to a mor being >>> proactive >&g has been a< about the co >>> I >>> will take it any day over >>> >>> On Wed, Nov 26, 2008 at 5:29 PM, >>> >wrote: >& >>> > We have had some trouble but they are planning them. We >>> received >>> > a letter they need to >> stopped >>> working >>> > completely. >>& >>> > Lynn Burton >>> > Lab Assoc. I >>> > Animal Disease Lab >>> > Galesburg, Il >>> > >>> > __________________________ >>> > >>> > From: histonet-bounces@lis of Tracey >>> Lenek >> >>> > To: histonet@l >>> > Cc: Martin Trotter; Joanna Bar >>> > Subject: [Histonet] Ventana Benchmark XTs< >>> > >>> > >>& >>> > >>> > We have had on-going since they >>> were & >>> > in February. The slide tray not once but >>> > twice and >>> > results with the temp experienced the same >>> > issu >>> > instrumentation? >>> > >>> > Tracey Lenek >>> >>> > Calgary Laboratory >>> > 403-770-3588 >>> > >> >>> > This messag use of the >>> &g privileged &g retransmission, >>> > disclosure is strictly prohibited. If you hav this message in >>> > error, please notify the se delete the > >>> original > >>> > ____________________ >>> > Histonet mailing list & >>> > htt >>> > >>> > >>> > ________________________________ >>> > Histonet mailing list >>> >>> > http://lists.ut >>> > >> >>> >>> >>> -- >>> The >>> _________________________________________ >>> Histonet mailing list >>> Histonet@lists >>> http://lists.utsouthwestern.edu/mailman >>> >>> ________________________ >> Histonet mailing list >> Histo >> http://lists.utsouthwestern.edu/ma >> > > ____________________ Histonet mailing list Histonet@lists.utso http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Sat Nov 29 08:40:26 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Nov 29 08:40:28 2008 Subject: [Histonet] Ventana Benchmark XTs References: <1589726357.931151227932039574.JavaMail.root@vms246.mailsrvcs.net> Message-ID: <3E77B77321BA4C7894EDC216DDA10A0C@yourxhtr8hvc4p> nope, the hound of Tucson ----- Original Message ----- From: Steven Hacker To: marktarango@gmail.com Cc: jnocito@satx.rr.com ; histonet@lists.utsouthwestern.edu ; Tracey.Lenek@cls.ab.ca ; Lynn.Burton@illinois.gov ; Joanna.Bartczak-McKay@cls.ab.ca ; Martin.Trotter@cls.ab.ca Sent: Friday, November 28, 2008 10:13 PM Subject: Re: Re: [Histonet] Ventana Benchmark XTs The Hound of the Baskerville? Nov 28, 2008 08:51:46 PM, marktarango@gmail.com wrote: Yup, been through that before. I made sure that we switched to BioCare's reagents on a dako-like stainer after that. I would think that'd be the only reason to hide your identity (fear of Ventana sending the hounds after you). On Fri, Nov 28, 2008 at 3:19 PM, Joe Nocito wrote: > no, they usually contact the CEO's of the labs to get their employees to > stop ragging on the company. Then again, you never know who is lurking in > the winds. > > JTT > ----- Original Message ----- From: "Mark Tarango" > To: "Histonet Alias" > Cc: ; "Martin Trotter" > Martin.Trotter@cls.ab.ca>; "Tracey Lenek" ; > "Burton,Lynn" ; "Joanna Bartczak-McKay" > Joanna.Bartczak-McKay@cls.ab.ca> > Sent: Friday, November 28, 2008 2:10 PM > Subject: Re: [Histonet] Ventana Benchmark XTs > > > I don't know how much the opinion of "The Unknown HT(ASCP)" counts. You >> aren't with Ventana I hope. >> >> Mark >> >> On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias >> >wrote: >> >> They are switching to a more reliable manufacturer. They are being >>> proactive >>> and switching them before they fail down the road. My XT has been a >>> workhorse but we know certain peoples opinions about the company on here. >>> I >>> will take it any day over the rest. >>> >>> On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn >>> >wrote: >>> >>> > We have had some trouble but they are planning to replace them. We >>> received >>> > a letter that they had changed vendors for parts and found they need to >>> > switch back. They did replace one section earlier when it stopped > >>> working >>> > completely. >>> > >>> > Lynn Burton >>> > Lab Assoc. I >>> > Animal Disease Lab >>> > Galesburg, Il 61401 >>> > >>> > ________________________________ >>> > >>> > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tracey >>> Lenek >>> > Sent: Wed 11/26/2008 10:59 AM >>> > To: histonet@lists.utsouthwestern.edu >>> > Cc: Martin Trotter; Joanna Bartczak-McKay >>> > Subject: [Histonet] Ventana Benchmark XTs >>> > >>> > >>> > >>> > Hi, >>> > >>> > We have had on-going thermal slide pad issues with our XTs since they >>> were >>> > installed >>> > in February. The slide tray assemblies have been replaced not once but >>> > twice and we are still having inconsistent >>> > results with the temp verifier slides. Has anyone experienced the same >>> > issues with this >>> > instrumentation? >>> > >>> > Thanks >>> > Tracey Lenek >>> > Tech III - Anatomic Pathology >>> > Calgary Laboratory Services >>> > 403-770-3588 >>> > >>> > ________________________________ >>> > This message and any attached documents are only for the use of the >>> > intended recipient(s), are confidential and may contain privileged >>> > information. Any unauthorized review, use, retransmission, or other >>> > disclosure is strictly prohibited. If you have received this message in >>> > error, please notify the sender immediately, and then delete the > >>> original >>> > message. Thank you. >>> > _______________________________________________ >>> > Histonet mailing list >>> > Histonet@lists.utsouthwestern.edu >>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > >>> > >>> > _______________________________________________ >>> > Histonet mailing list >>> > Histonet@lists.utsouthwestern.edu >>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > >>> >>> >>> >>> -- >>> The Unknown HT(ASCP) >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histonetalias <@t> gmail.com Sat Nov 29 10:03:18 2008 From: histonetalias <@t> gmail.com (Histonet Alias) Date: Sat Nov 29 10:03:28 2008 Subject: [Histonet] Ventana Benchmark XTs In-Reply-To: <5b6eb13e0811281210j7fd2f0d5w1c3283bd9bdead11@mail.gmail.com> References: <2DFAEEFF192A9141ABACFF88BE613BF30BBC56@EXMBXC1.crha.bewell.ca> <4b6c85510811281151w4aec5437ybf85920655ab9def@mail.gmail.com> <5b6eb13e0811281210j7fd2f0d5w1c3283bd9bdead11@mail.gmail.com> Message-ID: <4b6c85510811290803v7e52528bw2aee194670077f0@mail.gmail.com> Sorry I don't work for Ventana and I have no beef with them. It may disappoint some that I have not had a bad experience. So my opinion does not count because I have had a different experience with my XT than you? I use the name of The Unknown HT so I can express my opinions freely without my employer receiving phone calls because certain people don't like my opinions. On Fri, Nov 28, 2008 at 3:10 PM, Mark Tarango wrote: > I don't know how much the opinion of "The Unknown HT(ASCP)" counts. You > aren't with Ventana I hope. > > Mark > > On Fri, Nov 28, 2008 at 11:51 AM, Histonet Alias wrote: > >> They are switching to a more reliable manufacturer. They are being >> proactive >> and switching them before they fail down the road. My XT has been a >> workhorse but we know certain peoples opinions about the company on here. >> I >> will take it any day over the rest. >> >> On Wed, Nov 26, 2008 at 5:29 PM, Burton, Lynn > >wrote: >> >> > We have had some trouble but they are planning to replace them. We >> received >> > a letter that they had changed vendors for parts and found they need to >> > switch back. They did replace one section earlier when it stopped >> working >> > completely. >> > >> > Lynn Burton >> > Lab Assoc. I >> > Animal Disease Lab >> > Galesburg, Il 61401 >> > >> > ________________________________ >> > >> > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tracey >> Lenek >> > Sent: Wed 11/26/2008 10:59 AM >> > To: histonet@lists.utsouthwestern.edu >> > Cc: Martin Trotter; Joanna Bartczak-McKay >> > Subject: [Histonet] Ventana Benchmark XTs >> > >> > >> > >> > Hi, >> > >> > We have had on-going thermal slide pad issues with our XTs since they >> were >> > installed >> > in February. The slide tray assemblies have been replaced not once but >> > twice and we are still having inconsistent >> > results with the temp verifier slides. Has anyone experienced the same >> > issues with this >> > instrumentation? >> > >> > Thanks >> > Tracey Lenek >> > Tech III - Anatomic Pathology >> > Calgary Laboratory Services >> > 403-770-3588 >> > >> > ________________________________ >> > This message and any attached documents are only for the use of the >> > intended recipient(s), are confidential and may contain privileged >> > information. Any unauthorized review, use, retransmission, or other >> > disclosure is strictly prohibited. If you have received this message in >> > error, please notify the sender immediately, and then delete the >> original >> > message. Thank you. >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> > >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> >> >> >> -- >> The Unknown HT(ASCP) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > -- The Unknown HT(ASCP) From ancillarypath <@t> mac.com Sun Nov 30 10:17:51 2008 From: ancillarypath <@t> mac.com (ancillarypath@mac.com) Date: Sun Nov 30 10:18:04 2008 Subject: [Histonet] Job posting for IHC tech at Vitro Molecular Laboratories In-Reply-To: <492ee04e.1917400a.66f0.ffff8712SMTPIN_ADDED@mx.google.com> References: <492ee04e.1917400a.66f0.ffff8712SMTPIN_ADDED@mx.google.com> Message-ID: <890CA269-5B0A-4794-AEE6-7E5BD6AFC080@mac.com> Dear colleagues, Vitro Molecular Laboratories is in a need for an additional histotech with expertise in immunohistochemistry. The laboratory is based in South FL. The position is a full-time position, with potential for growth. Knowledge in basic and advanced IHC is required, particularly in troubleshooting. While the position does not require supervisory experience, we could modify the position both logistically and financially. We offer benefits including health and matching 401k, etc. The salary is generous and proportional to the level of experience. Interested colleagues should reply directly to me at their earliest convenience. Warmest regards, Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com From stevenk <@t> med.usyd.edu.au Sun Nov 30 22:28:15 2008 From: stevenk <@t> med.usyd.edu.au (Stephen KumJew) Date: Sun Nov 30 22:29:44 2008 Subject: [Histonet] Buffy coat preparation for immuno Message-ID: Hi from Down Under I was wondering if anyone had a method for preparing buffy coats for immuno. What we have done so far 1) is spun down whole blood, extracted the buffy coat and made a thrombin/plasma clot of the extracted buffy coat (minus RBCs). The clot was post fixed overnight in 10% formalin and subsequently paraffin processed. 5um Sections were cut and immuno done. The clumping cells did not stain, but the single ones on their own did. 2) To improve on fixation (assuming this was the problem with the unstained clumping cells), the buffy coat was collected and fixed in 10% formalin. The fixed buffy coat was washed in 0.9% NaCl twice, and then embedded in both a thrombin/plasma clot (unsuccessful), and an agar block. The agar block was paraffin processed. The subsequent immuno showed good staining in single WBC, and in one clump of cells. The other clump showed no staining and had a bit of cell damage. Thanks Stephen Pathology Sydney University From jkiernan <@t> uwo.ca Sun Nov 30 22:54:59 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sun Nov 30 22:55:03 2008 Subject: [Histonet] Buffy coat preparation for immuno In-Reply-To: References: Message-ID: Why go to all the trouble of embedding and sectioning a buffy coat specimen? What's wrong with diluting it in saline and then making smears or (better, if you have the equipment) cyto-centrifuge preparations? You'll get lots of slides. They can be fixed in methanol and the WBC stained with any conventional Romanowsky-Giemsa method, or immunohistochemically. You could even fix in formalin, if there's some special reason to do so. (You have to lower the pH of an ordinary blood stain if the fixative was formaldehyde.) I hope this message is readable; it is sent as plain text. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: Stephen KumJew Date: Sunday, November 30, 2008 23:30 Subject: [Histonet] Buffy coat preparation for immuno To: histonet@lists.utsouthwestern.edu > Hi from Down Under > > I was wondering if anyone had a method for > preparing buffy coats for > immuno. > > What we have done so far > > 1) is spun down whole blood, extracted the buffy coat and made a > thrombin/plasma clot of the extracted buffy coat (minus RBCs). > The clot was > post fixed overnight in 10% formalin and subsequently paraffin > processed.5um Sections were cut and immuno done. The clumping > cells did not stain, but > the single ones on their own did. > > 2) To improve on fixation (assuming this was the problem with > the unstained > clumping cells), the buffy coat was collected and fixed in 10% > formalin. The > fixed buffy coat was washed in 0.9% NaCl twice, and then > embedded in both a > thrombin/plasma clot (unsuccessful), and an agar block. > The agar block was > paraffin processed. The subsequent immuno showed good staining > in single > WBC, and in one clump of cells. The other clump showed no > staining and had a > bit of cell damage. > > Thanks > Stephen > Pathology > Sydney University > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet


> Thanks for responding.  Fixed in 10% f alcoh
> Surgipath. Cut at 5 microns Richard Allan's Chromaview Stain ki Trichrome.  Used the
> above meth routine Trichrome controls and all the muscle stained red as < BR>> they are supposed to be.  That's why I put ou the mu has
well.


> Thought ma formalin had something to do with the
staining.  Maybe longer than an hour in Bouin' continuing the
> Trichrome would help?

> Await your thoughts and info

> Re  < dated 11/8/2008 2:
> jkiernan@uw writes:

> style="PADDING-LEFT: 5px; MARGIN-LEFT: 5px; BO RDER-LEFT: blue 2px solid">>
> are numerous enough to make years. As a genera lity, trichrome
> methods
> do not work formaldeh Postfixation of th Bouin
> is picric acid us
> jus are
> published reports that iodine and
> buffer will improve trichrome
&g staining of paraffin sections of
> formaldehyde-fixe tissue. See Yu &
> Chapman 2003 J. 26(2)
>  
> "Trichrome" has been applied to several staini ng
> techniques that use two
> or more dye trichrome met phosphomolybdic or phosphotun to
> enable the sta cytoplasm by
> anionic dye
> contrasting colours: blue or
> collagen, and red for cytoplasm
&g (including smooth & striated muscle). A
> third anionic dye, typically yellow
> or orange, may Instructions for < all
> tex and
> histotechnology.  
> John
> Kiern an
> Anatomy, UWO
> London, Canada
Message
&g angelafogg@aol.com
> 7, 2008
> 20:17
& [Histonet] Trichrome Question
> To:
> histonet@lists.utsouthwestern.edu
>
> >
> > Performed a trichro
&
> Muscle st > red.?What happened?
> fixation react this way?
& Hope someone can shed some light on
> this .
> > Regards,
> > Angela< ___________ _______________________ 5F Histonet m Histonet@lists.
> http:// lists.utsouthwestern.edu/mailman/listinfo/histonet
> < /FONT>