[Histonet] Embedding centers and Hematoxylin

[Weems, Joyce]

We use Tissue Tek from Sakura. We just replaced one we had since the
70's. The old ones lasted forever, but I don't know how the new ones
will do. 

We use Richard Allen Hematoxylin 7211, which is most nearly like the old
Harris hematoxylin that I have found. We use it regressivly and get a
nice bright stain without blue goblet cells. 

Best wishes, 
Joyce

Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Rathborne, Toni
Sent: Saturday, May 31, 2008 12:48 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Embedding centers and Hematoxylin


I have a few questions for everyone. 1) How many years do you get from
your embedding centers, and what make are they? 2) What type of
hematoxylin does everyone use for their H&E's? In general, do you prefer
a progressive or regressive stain?
Thanks in advance for your replies.

Toni


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