FW: [Histonet] Immuno Controls

Fri May 30 11:24:25 CDT 2008

I don't think that it is the role of HistoNet of convincing anybody to do or stop doing something. Here a "full package" of opinions and experiences is presented and it is the individual who will have to "digest" what is posted, pros and cons, and act according with the proper reasoning on each issue.
  My question to you could be: if a case turned out negative in one of those runs with a sole (+) control, was it negative because it was really negative or because that particular section of that case did not receive all the reagents?
  You cannot answer that question with your method.
  René J.

"Charles, Roger" <rcharles <@t> state.pa.us> wrote:
  Hello all,
I've read many posts on this subject of people putting controls on each
slide because machines do make mistakes. I do not do this; I use one
slide as a control (which contains both negative and positive tissue)
for each run or each different antibody. What I would like to know from
those putting controls on each slide is if they ever seen one or more
slides where the control did not work but yet controls on other slides
from the same run and same antibody did. Convince me to change my ways.
Thanks 

Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mickie
Johnson
Sent: Friday, May 30, 2008 10:34 AM
To: 'Cheri Miller'; 'Happel, James F.';
Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Immuno Controls

Hi Histonetters,

I would like to add my 2 cents worth. When I started IHC it was by hand
and
the tech was sure they had put primary Ab, etc on each slide. Then we
went
automated with a Ventana ES. We learned the hard way that dispensers may
or
may not dispense their contents and that this could vary from slide to
slide
on the same run! This is when we began to put a control slide on each
positive patient slide for each antibody run on that patient and block.
Machines make mistakes too.

Thanks.

Best Regards,

Mickie

Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
& Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
FAX 509-624-3926
Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com 
Email: mickie25 <@t> netzero.net

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheri
Miller
Sent: Friday, May 30, 2008 4:08 AM
To: 'Happel, James F.'; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Immuno Controls

I put control tissue on each slide I stain except the neg controls.

Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne. 
402 738 5052
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Happel,
James F.
Sent: Thursday, May 29, 2008 2:11 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Immuno Controls

Good Day Histonetters! For immuno cases, how are you handling the
control
tissue question? Are you putting a piece of control tissue on each
slide or
are
you using a control slide for each ab performed on a given day. We are
using
Ventana'e Benchmark for the lion's share of our immunos.

Thanks!

James Happel
Massachusetts General Hospital

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