[Histonet] Immuno Controls

Fri May 30 10:56:59 CDT 2008

If your instrument is going to "forget" to dispense even once in its
lifetime, does that justify not using a control on each slide? Maybe I'm
being a little dramatic, but I wouldn't want my tissue on the slide that
day.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Poteete,
Jacquie A.
Sent: Friday, May 30, 2008 11:05 AM
To: Charles, Roger; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Immuno Controls

Yes, Roger, in fact I have seen this happen twice in the last week.  One
of my elderly instruments chose to "forget" to dispense reagent both
times.  I've also seen instruments dispense fully, partially, or not at
all during the same run.  Yes, service calls were generated, and
everything was repeated.  Of course, the instrument's "brother" started
its run by itself without us pushing the "START" button, but that's
another story.....and new instrumets are on order.

Jacquie Poteete MT(ASCP)QIHC
Lead Technologist, IHC Laboratory
Saint Francis Hospital, Tulsa, OK
japoteete <@t> saintfrancis.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Charles,
Roger
Sent: Friday, May 30, 2008 9:54 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: FW: [Histonet] Immuno Controls

Hello all,
I've read many posts on this subject of people putting controls on each
slide because machines do make mistakes.  I do not do this; I use one
slide as a control (which contains both negative and positive tissue)
for each run or each different antibody.  What I would like to know from
those putting controls on each slide is if they ever seen one or more
slides where the control did not work but yet controls on other slides
from the same run and same antibody did.  Convince me to change my ways.
Thanks 

Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mickie
Johnson
Sent: Friday, May 30, 2008 10:34 AM
To: 'Cheri Miller'; 'Happel, James F.';
Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Immuno Controls

Hi Histonetters,

I would like to add my 2 cents worth. When I started IHC it was by hand
and the tech was sure they had put primary Ab, etc on each slide. Then
we went automated with a Ventana ES. We learned the hard way that
dispensers may or may not dispense their contents and that this could
vary from slide to slide on the same run! This is when we began to put a
control slide on each positive patient slide for each antibody run on
that patient and block. Machines make mistakes too.

Thanks.

Best Regards,

Mickie

Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
FAX   509-624-3926
Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com 
Email: mickie25 <@t> netzero.net

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheri
Miller
Sent: Friday, May 30, 2008 4:08 AM
To: 'Happel, James F.'; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Immuno Controls

I put control tissue on each slide I stain except the neg controls.

Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne. 
402 738 5052
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Happel,
James F.
Sent: Thursday, May 29, 2008 2:11 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Immuno Controls

Good Day Histonetters!  For immuno cases, how are you handling the
control tissue question?  Are you putting a piece of control tissue on
each slide or are you using a control slide for each ab performed on a
given day.  We are using Ventana'e Benchmark for the lion's share of our
immunos.

Thanks!

James Happel
Massachusetts General Hospital

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