[Histonet] IHC Controls

[Sebree Linda A.]

One of the reference labs we use does just that, fitting their positive
control tissue wherever on the slide it will fit.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon
Campbell
Sent: Friday, May 30, 2008 9:49 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC Controls


 

I would like to ask a question about putting control tissue on
"unstained" slides. We occasionally receive unstained slides and need to
run IHC or ISH on them. We are using the Ventana Benchmark and Benchmark
XT. To save money we are wondering if it is ok to float control tissue
onto the existing tissue slide. Is there a problem with carefully
turning the slide so that the top (end with the writing) is dipped into
the waterbath and the positive control floated onto the top of the glass
portion?

Thank you for your help.

 

Sharon Campbell, HTL(ASCP)CM, BSBM

Histology Supervisor

Celligent Diagnostics, LLC

Formerly Pathology Associates Services

101 East W.T. Harris Blvd, Suite 1212

Charlotte, NC  28262

(704) 549-8444 x100

sharonc <@t> celligent.net

 

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