[Histonet] Sarcoplasmic reticulum.

Lee & Peggy Wenk lpwenk <@t> sbcglobal.net
Thu May 29 19:50:14 CDT 2008


If the muscle is a frozen section:
- Do a NADH-tetrazolium reductase which will show the mitochondria and the
sarcoplasmic reticulum. Also do a SDH which which demonstrate only the
mitochondria. Compare the two sections.
- And throw in a modified Gomori trichrome for giggles and kicks.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ian
Montgomery
Sent: Thursday, May 29, 2008 10:15 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sarcoplasmic reticulum.

            Just been asked, "is it possible to stain the sarcoplasmic
reticulum at the light level." EM it jumps out at you but I'm a bit stumped
for LM. CaATPase, mmmm, any thoughts?

Ian.

 

Dr. Ian Montgomery,

Histotechnology,

I.B.L.S. Support Unit,

Thomson Building,

University of Glasgow,

Glasgow,

G12 8QQ.

 

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