[Histonet] Formalin alternative

[Amos Brooks]

Interesting,
     After my saying it worked well :-) What tissues were you working with
that the morpho was poor? (So I don't try it & get the same results) We
don't use it for everything, so I guess it could be tissue dependant. I
swear it is the shiznit for these mouse lungs.

Different strokes I guess,
Amos


Message: 1
Date: Wed, 28 May 2008 10:32:33 -0500
From: Jackie M O'Connor
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>
Subject: Re: [Histonet] Formalin alternative fixative for rodent and
       large   animal
To: stephanie.d.rivera <@t> gsk.com
Cc: histonet <@t> lists.utsouthwestern.edu,
       histonet-bounces <@t> lists.utsouthwestern.edu
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       <OFD527EDEC.DFDE0004-ON86257457.00551389-86257457.005565B0 <@t> abbott.com
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In my opinion, for pharma research, there is no worthwhile formalin
substitute that will give consistent IHC results.   I have tried STF
(which was discontinued) and other subs - finally went back to formalin.
With STF, the morphology of routine H+E's was horrible.    I'm sure there
are differing opinions out there - after all, this is histonet.

Jackie O'