[Histonet] HIER to repair dried specimens?

[crochieresteve <@t> aol.com]

I'm familiar with rehydrating mummy tissue from my years at AFIP. We used formol-glycol for several days prior to processing. I didn't think boiling slides in buffer would "repair" "cooked" tissue, but it seems to be so.
Thanks
s


-----Original Message-----
From: Bryan Hewlett <bhewlett <@t> cogeco.ca>
To: histonet <@t> pathology.swmed.edu; crochieresteve <@t> aol.com
Sent: Thu, 22 May 2008 11:38 am
Subject: Re: [Histonet] HIER to repair dried specimens?


It's called re-hydration!?
Similar techniques are used for partial restoration of mummified tissue for histological examination.?
?
Bryan?
\?
?
----- Original Message ----- From: <crochieresteve <@t> aol.com>?
To: <histonet <@t> pathology.swmed.edu>?
Sent: Thursday, May 22, 2008 10:49 AM?
Subject: [Histonet] HIER to repair dried specimens??
?
>I have accidentally stumbled on an unexplainable "by product" of HIER in >citrate buffer, pH6.0.?
> We had some specimens that had unfortunately air dried prior to fixation > and processing and therefore looked terrible. The H&E was practically > unreadable due to drying artifact. In an effort to see something > worthwhile, IHC stains were ordered. The hematoxylin counterstain, > following retrieval showed much better nuclear detail than the original > H&E. Several additional slides were cut and stained with H&E following > HIER. They were much better. This has been repeated on a few other > specimens with similar resu?
> lts. Any ideas as to how or why this is working??
> thanks?
> sc?
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