FW: [Histonet] removing tissue from already H and E stained slides.

[Patti Loykasek]

------ Forwarded Message
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Date: Wed, 21 May 2008 09:07:30 -0700
To: AMH-HistoLab <amh-histolab <@t> amh.org>
Subject: Re: [Histonet] removing tissue from already H and E stained slides.

Is your pretreatment for ER & PR the same? If so, there's no need to remove
a section. The slide can be stained manually. Simply pretreat the slide
(with appropriate controls of course), use a barrier pen around each
section, carefully apply each antibody - 1 per section- & perform your
detection etc.. as usual. If your H&E is on a non-adhesive slide you do run
the risk of losing your sections during pretreatment. Hope this helps.


Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA





> I have a situation where a pathologist needs an Er and PR IHC done on a
> previous H and E stained slide. The slide has two sections on it and she
> asked if one section  can be removed to another slide to perform ER on
> one slide and PR on the other. Does anyone know of a solution that will
> remove tissue safely from a slide after H and E stained and not
> interfere with IHC?
> 
> Abington Memorial Hospital
> 1200 Old York Rd
> Abington, PA. 19001
> 
> 
> 
> ****************** CONFIDENTIALITY NOTICE **********************
> 
> This e-mail contains LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION
> intended only for the use of the recipient named above.  If you are not
> the intended recipient, you are hereby notified that any dissemination or
> copying of this e-mail is strictly prohibited.  If you have received this
> e-mail in error, please notify the transmitting hospital by telephone or
> e-mail and delete the original e-mail received in error.
> 
> THIS INFORMATION HAS BEEN DISCLOSED TO YOU FROM RECORDS WHOSE
> CONFIDENTIALITY IS PROTECTED BY STATE AND FEDERAL LAW.  ANY FURTHER
> DISCLOSURE, COPYING, DISTRIBUTION OR ACTION TAKEN IN RELIANCE ON THE
> CONTENTS OF THESE DOCUMENTS WITHOUT THE PRIOR WRITTEN CONSENT OF THE
> PERSON TO WHOM IT PERTAINS IS PROHIBITED.  YOU ARE REQUIRED TO DESTROY
> THE INFORMATION AFTER THE STATED NEED HAS BEEN FULFILLED.
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

------ End of Forwarded Message



This e-mail message, including any attachments, is for the sole use of the 
intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.