[Histonet] modified Gomori stain

[Hofecker, Jennifer L]

Hi there.
I have two suggestions for you. The first would be to check the pH of
your Gomori trichrome solution. Strange things happen to muscles when
the pH strays from 3.4! The second suggestion would be to just skip the
acid differentiation, provided the sections are green when they come out
of the stain.  I did that once, accidentally, and the pathologist was
able to interpret the slides. If the sections are predominately red
after the staining step, check the pH.  Please let me know if you need
any further information.
Good Luck!  


Jennifer L. Hofecker HT(ASCP)
Vanderbilt University Medical Center
Division of Neuropathology
Nashville, TN
 
ph 615.343.0083
fax 615.343.7089

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of manal
galal
Sent: Wednesday, May 21, 2008 8:55 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] modified gomori stain


   Hi

    I need help with modified gomori stain for muscle biopsies. I cant
   seem to get the muscle fiber to turn green. everytime I put the
acetic
   acid for differentiation, the fibers loose all the color. can anyone
   help me.



                Manal Galal


                  cairo
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