[Histonet] AEC chromogen - coverslipping

[sheila adey]

We were of the understanding that we could just use the aqueous mountant with the cover glass in the over and they were good to go?Sheila Adey HT MLTPort Huron HospitalMichigan> From: gayle.callis <@t> bresnan.net> To: Rcartun <@t> harthosp.org; histonet <@t> lists.utsouthwestern.edu> Date: Fri, 16 May 2008 13:18:31 -0600> Subject: Re: [Histonet] AEC chromogen - coverslipping> CC: > > Richard,> > We have used the liquid mounting media, Crystal Mount from Biomeda - > ThermoFisher used to carry this. Do the liquid coverslip first, let it dry > according to directions i.e. several ways with heat, then mount a permanent > coverglass over the top of this. AEC is preserved.> > If you try to look at the section before mounting a permanent coverslip, > it can look a bit out of focus. Make sure the media covers the section > smoothly and as flat at possible to avoid the "waves" of media.> > Someone recently posted the contact for Biomeda on Histonet on the chance > ThermoFisher is not selling Crystal Mount anymore. There are other > companies that supply this type of mounting media but not sure which > vendors.> > Good luck> > Gayle M. Callis> HTL/HT/MT(ASCP)> Bozeman MT 59715> > ----- Original Message ----- > From: "Richard Cartun" <Rcartun <@t> harthosp.org>> To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>> Sent: Friday, May 16, 2008 11:22 AM> Subject: [Histonet] AEC chromogen - coverslipping> > > If you use AEC as a chromogen for IHC staining, what are using to protect it > before putting the slides into xylol for permanent coverslipping?> > Thanks!> > Richard> > Richard W. Cartun, Ph.D.> Director, Immunopathology & Histology> Assistant Director, Anatomic Pathology> Hartford Hospital> 80 Seymour Street> Hartford, CT 06102> (860) 545-1596> (860) 545-0174 Fax> > Confidentiality Notice> > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential or proprietary > information which is legally privileged. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are not the intended > recipient, please promptly contact the sender by reply e-mail and destroy > all copies of the original message.> > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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