[Histonet] Calcium oxylate stain

[Gayle Callis]

Pizzolata's stain for calcium oxalate.  I has been discussed on Histonet in 
the past, and you may be able to pick up the method on the web.  Sheehan and 
Hrapchak Theory and Practice of Histotechnology, second edition has the 
method.  Very easy to do.  We ran the von Kossa on an adjacent section to 
distinguish between calcium oxalate and other calcium salt deposits, and 
eliminate one or the other for diagnostic purposes.

A good calcium oxalate positive control is a dog or cat kidney that drank 
antifreeze containing ethylene glycol.  The crystals are a bit refractile 
looking in an H&E section.  A local veterinary diagnositic laboratory may 
have tissues available.

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT 59715


----- Original Message ----- 
From: "Mary Lloyd" <lloyd.3 <@t> osu.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, May 16, 2008 12:04 PM
Subject: [Histonet] Calcium oxylate stain


My oral pathologist is requesting a specific stain for calcium oxylate.
I have done von kossa for calcium but he wants something more specific.
I would appreciate any help.  Thanks Mary
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