[Histonet] Help identifying LS174T tumor cells

[jstaruk]

OK, I've been working on this for over a week and finally decided I need
help.  LS174T tumor cells (a human colon cancer cell line) were implanted
into mouse lymph nodes.  I'm trying to immuno-stain these implanted cells.
I tried a pan cytokeratin antibody and a CK20 cytokeratin antibody and
neither worked.  Has anyone here ever worked with this cell line?  Any
suggestions on how to differentiate these cells from the normal mouse cells?

Thanks!

Jim

_____________________
    Jim Staruk
Mass Histology Service
www.masshistology.com