[Histonet] 4% paraformaldehyde all over again
Downs, Heather M.
HDOWNS <@t> PARTNERS.ORG
Tue May 13 13:26:28 CDT 2008
We purchase paraformaldehyde in powder form, from Fisher, and make our own 4%
paraformaldehyde. We use it for perfusion, of animals and making PLP for our
biopsies.
Heather
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Sent: Tuesday, May 13, 2008 1:03 PM
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Subject: Histonet Digest, Vol 54, Issue 17
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Today's Topics:
1. C =Con(with) S means Sans(without)-Latin origin (Madary, Joseph)
2. RE: C =Con(with) S means Sans(without)-Latin origin
(Bonner, Janet)
3. ICC problem (Karla Arrington)
4. Bone Marrow Staining (Karla Arrington)
5. Re: Bone Marrow Staining (Rene J Buesa)
6. Re: IHC Background Staining (Victoria Baker)
7. non specific staining (Teisha Robertson)
8. RE: RE: Latin - Thank goodness! (Ingles Claire)
9. RE: RE: [Histonet] I need more ammunition (Thomas Jasper)
10. Method validation (Oto, Carrie)
11. Re: C =Con(with) S means Sans(without)-Latin origin (Joe Nocito)
12. Re: 4% paraformaldehyde all over again (John Kiernan)
13. thanks (louise renton)
14. RE: 4% paraformaldehyde all over again (Smith, Allen)
15. Catalase Staining for Liver Aspirates Help Please (Amy Porter)
16. Amended reports- Cerner CoPath users (Angela Bitting)
17. Standardized Microtomes (Nancy Schmitt)
18. Apipophilin and MCM (Michele Wich)
19. rubber stoppers & tubing (Atoska Gentry)
20. RE: Standardized Microtomes (Liz Chlipala)
21. Re: Standardized Microtomes (Jackie M O'Connor)
22. Paraffin thickness standard or measurement? (amy rizzo)
23. RE: Standardized Microtomes (Joyce Cline)
----------------------------------------------------------------------
Message: 1
Date: Mon, 12 May 2008 13:08:59 -0400
From: "Madary, Joseph" <MadaryJ <@t> MedImmune.com>
Subject: [Histonet] C =Con(with) S means Sans(without)-Latin origin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E5B1C3D1305EFD4C9CA7F63094135F5E8A2C4B <@t> MD1EV002.medimmune.com>
Content-Type: text/plain; charset="us-ascii"
Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Laboratory
One Medimmune Way
Gaithersburg, MD 20878
ph 301.398.4745/6360
fx 301.398.9745
To the extent this electronic communication or any of its attachments
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------------------------------
Message: 2
Date: Mon, 12 May 2008 13:19:14 -0400
From: "Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>
Subject: RE: [Histonet] C =Con(with) S means Sans(without)-Latin
origin
To: "Madary, Joseph" <MadaryJ <@t> MedImmune.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<5F31F38C96781A4FBE3196EBC22D47807F2647 <@t> fhosxchmb006.ADVENTISTCORP.NET>
Content-Type: text/plain; charset=iso-8859-1
OK, I use w/ = with, and w/o = without. -American
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Madary, Joseph
Sent: Mon 5/12/2008 1:08 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] C =Con(with) S means Sans(without)-Latin origin
Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Laboratory
One Medimmune Way
Gaithersburg, MD 20878
ph 301.398.4745/6360
fx 301.398.9745
To the extent this electronic communication or any of its attachments
contain information that is not in the public domain, such information
is considered by MedImmune to be confidential and proprietary, and
expected to be used only by the individual(s) for whom it is intended.
If you have received this electronic communication in error, please
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To the extent this electronic communication or any of its attachments contain
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Message: 3
Date: Mon, 12 May 2008 12:56:58 -0700 (PDT)
From: Karla Arrington <freckles9660 <@t> yahoo.com>
Subject: [Histonet] ICC problem
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <251789.53346.qm <@t> web32506.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello all..
I currently perform IHC's by hand. Although they are beautiful, I do get
a faint background staining inside the circle of the hydropholic pen. It
looks like excess DAB stain. I do not wipe around the tissues causing no
static. Any suggestions on how to fix this?
Karla Arrington
________________________________________________________________________________
____
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------------------------------
Message: 4
Date: Mon, 12 May 2008 13:04:05 -0700 (PDT)
From: Karla Arrington <freckles9660 <@t> yahoo.com>
Subject: [Histonet] Bone Marrow Staining
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <92592.51349.qm <@t> web32502.mail.mud.yahoo.com>
Content-Type: text/plain; charset=us-ascii
I currently use 10% NBF for fixing bone marrows (core and clot). Although lately
the sections appear "washed out". I previously used B-5 Fixative. Is there a
better
fixative for fixing bone marrows so their cells are crisp and clear?
Karla Arrington
freckles9660 <@t> yahoo.com
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------------------------------
Message: 5
Date: Mon, 12 May 2008 13:17:13 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Bone Marrow Staining
To: Karla Arrington <freckles9660 <@t> yahoo.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <604837.79006.qm <@t> web65710.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
The "washed out" appearance most likely has nothing to do with fixation. Check
if you are heating the sections BEFORE they are completely drained off. That can
cause that artifact.
The only thing you have to be aware of when using NBF to fix BM specimens is
to control the pH on the staining solutions (specially the Giemsa).
René J.
Karla Arrington <freckles9660 <@t> yahoo.com> wrote:
I currently use 10% NBF for fixing bone marrows (core and clot). Although
lately
the sections appear "washed out". I previously used B-5 Fixative. Is there a
better
fixative for fixing bone marrows so their cells are crisp and clear?
Karla Arrington
freckles9660 <@t> yahoo.com
________________________________________________________________________________
____
Be a better friend, newshound, and
know-it-all with Yahoo! Mobile. Try it now.
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Message: 6
Date: Mon, 12 May 2008 16:33:38 -0400
From: "Victoria Baker" <bakevictoria <@t> gmail.com>
Subject: Re: [Histonet] IHC Background Staining
To: "Karla Arrington" <freckles9660 <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<4f016b690805121333m20c00719v77da5f74d5645c28 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
What everyone else is saying is true and you will need to check your
procedure carefully. Manual staining is a technique that is difficult
with some antibodies and especially when working with a large slide
number.
One of the things that I did was eliminate using a pap pen. I used 2
X 2 strips of parafilm that I cut in strips of 1 X 2 and layed them on
the tissue/slide after the reagent has been applied. To remove I
dipped the slides in buffer and the film would slide right off.
Good luck. I did IHC manually for a long time and it is not always easy.
Vikki
On 5/9/08, Karla Arrington <freckles9660 <@t> yahoo.com> wrote:
> I do Immuno's by hand and I am getting background DAB precipitate on the
inside circle of my sections.
> What is causing this and how do I get rid of it?
>
>
>
________________________________________________________________________________
____
> Be a better friend, newshound, and
> know-it-all with Yahoo! Mobile. Try it now.
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------------------------------
Message: 7
Date: Mon, 12 May 2008 14:27:43 -0700 (PDT)
From: Teisha Robertson <tshrobertson <@t> yahoo.com>
Subject: [Histonet] non specific staining
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <605614.77385.qm <@t> web62515.mail.re1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
how do you eliminate non specific staining in olfactory bulb sections?
---------------------------------
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Message: 8
Date: Mon, 12 May 2008 16:39:43 -0500
From: "Ingles Claire" <CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] RE: Latin - Thank goodness!
Cc: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<08A0A863637F1349BBFD83A96B27A50A120128 <@t> uwhis-xchng3.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="iso-8859-1"
I'm a youngster, but I also bought a latin dictionary just for the medical
terminology aspect. Good thing I didn't have to take it. It is interesting
stuff, but I'm terrible at learning languages.
Claire
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Smith, Allen
Sent: Mon 5/12/2008 11:51 AM
To: 'Breeden, Sara'
Cc: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: Latin - Thank goodness!
Latin is also useful in law. (A sponge left in the pelvic cavity is a case of
"Res ipsa loquitur.") Latin also helps in puzzling out articles in Spanish or
Portuguese. Most of all Latin gives access to a great literature that has been
rather poorly translated. No translation of the Aeneid give one any conception
of the magnificence of the original.
------------------------------
Message: 9
Date: Mon, 12 May 2008 15:52:06 -0700
From: "Thomas Jasper" <tjasper <@t> copc.net>
Subject: RE: RE: [Histonet] I need more ammunition
To: "WAYNE HOLLAND" <jackdodo <@t> msn.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<90354A475B420441B2A0396E5008D4965E20AF <@t> copc-sbs.COPC.local>
Content-Type: text/plain; charset="US-ASCII"
Hi Wayne,
I know I already sent you a reply on this topic (off-line). Just had
one more thought...you might want to point out to the "powers that be"
that your organization is running a HUGE risk for the ULTIMATE in legal
nightmares. Nothing gets the attention of "higher ups" quicker than
$$$$$$'s and lawsuits. In the end Wayne, if you do not get anywhere
with this group, I would seriously consider seeking employment
elsewhere. That's not always the most desirable move, but let's face
it...you are currently in a position and a market, which makes you a hot
commodity. Secondly, you don't need to be implicated in any legal
entanglements due to poor decisions (or lack of them) by the folks in
authority where you work. Watch out for yourself man it's tough to
speak truth to power. I pity the poor patients as well.
Good luck,
Tom J.
Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjasper <@t> copc.net
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of WAYNE
HOLLAND
Sent: Saturday, May 10, 2008 5:31 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: FW: RE: [Histonet] I need more ammunition
I need more ammunition, please!!
>From: WAYNE HOLLAND
>Sent: Fri 5/9/2008 1:47 AM
>To: Histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] I need your help
>
>
>Everyone, I have started a new job. I have many things that need fixed.
>I have a gross room that are using regular cassettes metal tops and
>they are wrapping all of the derm work in yes wet lens paper and
>leaving them on the countertop by the hoods for periods of up to 45
>minutes. I know this is not good for obvious reasons. I need your
>comments asap to help make them understand, again for obvious reasons.
>I have been doing this for 28 years and I need other field associates
>to back me up. Most of these specimen are very small. HELP!
>
>
>
>_______________________________________________
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>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>NOTICE OF CONFIDENTIALITY
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------------------------------
Message: 10
Date: Mon, 12 May 2008 16:26:06 -0700
From: "Oto, Carrie" <Carrie.Oto <@t> clinlab.ucsfmedctr.org>
Subject: [Histonet] Method validation
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<00EC53C1C120524B80A74AC951675233187E2C <@t> CB-LIS-APSVR-1.ucsfmedicalcenter.org>
Content-Type: text/plain
Our Histology lab will be moving from one campus to another, 2 seperate
CLIA's. Accrediting agency is Joint Commission. What, if any, has other
labs done for method validation of histology equipment and reagents? For
example, processor, stainer, IPOX stains, etc. Any and all information is
appreciated especially if there is specific information for California labs.
------------------------------
Message: 11
Date: Mon, 12 May 2008 20:56:18 -0500
From: "Joe Nocito" <jnocito <@t> satx.rr.com>
Subject: Re: [Histonet] C =Con(with) S means Sans(without)-Latin
origin
To: "Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>, "Madary, Joseph"
<MadaryJ <@t> MedImmune.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <006b01c8b49c$898769b0$0302a8c0 <@t> yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
yeah,
you go girl
JTT
----- Original Message -----
From: "Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>
To: "Madary, Joseph" <MadaryJ <@t> MedImmune.com>;
<histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, May 12, 2008 12:19 PM
Subject: RE: [Histonet] C =Con(with) S means Sans(without)-Latin origin
OK, I use w/ = with, and w/o = without. -American
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Madary, Joseph
Sent: Mon 5/12/2008 1:08 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] C =Con(with) S means Sans(without)-Latin origin
Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Laboratory
One Medimmune Way
Gaithersburg, MD 20878
ph 301.398.4745/6360
fx 301.398.9745
To the extent this electronic communication or any of its attachments
contain information that is not in the public domain, such information
is considered by MedImmune to be confidential and proprietary, and
expected to be used only by the individual(s) for whom it is intended.
If you have received this electronic communication in error, please
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------------------------------
Message: 12
Date: Tue, 13 May 2008 02:37:16 -0400
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] 4% paraformaldehyde all over again
To: Jackie M O'Connor <Jackie.O'Connor <@t> abbott.com>
Cc: Histonet <Histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID: <fc18db3b96d7.4828fedc <@t> uwo.ca>
Content-Type: text/plain; charset="us-ascii"
There is no such thing as 4% paraformaldehyde. Nobody can sell or
us nauseam. said, in 3 words, vomit". Latin is concise
formaldehyde is a real solution, and solution is to heat 40 grammes
of litre of water, with an alkaline catalyst to sp hydrolysis.
Paraformaldehyde exists only as a solid substan which is insoluble in
water. All this has been in all the textbooks and manuals for 40+
years.
<BR> <BR>Joh UWO<BR>London, Canada<BR> Original Message
-----<BR>From: <Jackie.O'Connor <@t> abbott.com>< Monday, May 12,
2008 10:52<BR>Subject: [Histo 4% paraformaldehyde all over again<BR>To:
Histonet < Histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> li sts.utsouthwestern.edu<BR><BR>> Will vendors who
cont quantities < again, please. < <BR>> Jackie O'Conn
Labs<BR>> _________ _______________________ 5F
Histonet mail Histonet <@t> lists.utsouthwestern.edu<BR>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Tue, 13 May 2008 11:02:22 +0200
From: "louise renton" <louise.renton <@t> gmail.com>
Subject: [Histonet] thanks
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<e483362e0805130202t4d4771aeg7eb2f1508a7f4a56 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
thanks to all who took the time to respond to my OT question. Now i can
sleep nights..........
--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
------------------------------
Message: 14
Date: Tue, 13 May 2008 09:07:41 -0400
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: RE: [Histonet] 4% paraformaldehyde all over again
To: 'John Kiernan' <jkiernan <@t> uwo.ca>
Cc: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E4132130AC2F764D8C173C5400D530426748D14E9E <@t> exchsrv02.barrynet.barry.edu>
Content-Type: text/plain; charset="us-ascii"
It appears that Dr. Kiernan is another victim of Microsoft office 2007. Could a
users' coalition large enough to boycott it effectively be formed?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of John Kiernan
Sent: Tuesday, May 13, 2008 2:37 AM
To: Jackie M O'Connor
Cc: Histonet; histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] 4% paraformaldehyde all over again
There is no such thing as 4% paraformaldehyde. Nobody can sell or
us=uch a non-substance!<BR>See Histonet archives passim ad
nauseam.=6nbsp; (That's how the ancient Romans
said, in 3 words,=2on and on, until you want to
vomit". Latin is concise=<BR> <BR>4%
formaldehyde is a real solution, and =e of the ways of making the
solution is to heat 40 grammes of =raformaldehyde in a
litre of water, with an alkaline catalyst to sp?d up the
hydrolysis. Paraformaldehyde exists only as a solid substan?,
which is insoluble in water. All this has been in all the
textbooks and manuals for 40+ years.
<BR> <BR>Joh=iernan<BR>Anatomy,
UWO<BR>London, Canada<BR>=D = = = = <BR>-----
Original Message -----<BR>From: =ckie M O'Connor
<Jackie.O'Connor <@t> abbott.com><=>Date:
Monday, May 12, 2008 10:52<BR>Subject: [Histo=t]
4% paraformaldehyde all over again<BR>To: Histonet <
Histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> li sts.utsouthwestern.edu<BR><BR>> Will vendors who
cont?ted me off line regarding supplying vast <BR>>
quantities <=>> of 4% paraformaldehyde contact me
again, please. <=>> Thanks.<BR>>
<BR>> Jackie O'Conn=<BR>> Abbott
Labs<BR>> _________ _______________________
5F=F_____________<BR>>
Histonet mail=g list<BR>>
Histonet <@t> lists.utsouthwestern.edu<BR>=6gt;
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Tue, 13 May 2008 10:49:40 -0400
From: "Amy Porter" <portera <@t> msu.edu>
Subject: [Histonet] Catalase Staining for Liver Aspirates Help Please
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: miyakaw2 <@t> msu.edu
Message-ID: <001201c8b508$92a98c50$8e7a0923 <@t> histolab>
Content-Type: text/plain; charset="iso-8859-1"
Hello to all - I am looking for an enzyme staining method to demonstrate
peroxisomes (catalase specifically) in Canine Liver aspirate smears. If anyone
out there has anything they would be will to share it would be so appreciated.
I have spent a great deal of time searching on the web and have not yet found a
methodology. Thanks in advance for any assistance. Amy
Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg. Rm #2133
East Lansing, MI 48824-3320
Phone: (517) 884-5026
Fax: (517) 432-1368
Email: portera <@t> msu.edu
Web: www.humanpathology.msu.edu
------------------------------
Message: 16
Date: Tue, 13 May 2008 11:23:17 -0400
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: [Histonet] Amended reports- Cerner CoPath users
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <48297A25.2B7F.00C9.0 <@t> geisinger.edu>
Content-Type: text/plain; charset="us-ascii"
I've got a question for users of Cerner Copath. Do you have CoPath set up so
that when a report is amended the original diagnosis remains visible along with
the amended diagnosis? Also, is there some type of flag at the top of the report
to alert a physician that there have been changes to the report? Thanks for your
help on this one.
Angie
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
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TEL;WORK:570-271-6844
ORG:;Histology
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------------------------------
Message: 17
Date: Tue, 13 May 2008 11:28:24 -0500
From: Nancy Schmitt <nancy_schmitt <@t> pa-ucl.com>
Subject: [Histonet] Standardized Microtomes
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9FC023A4AB52BB4D87DC6456081A822C087C8D <@t> mercury.pa-ucl.com>
Content-Type: text/plain; charset="iso-8859-1"
Good Morning to all
Does anybody standardize microtomes so they all cut at same angle? Is this
impossible? We only cut control blocks on one microtome because they are
each at a little different angle. Would be nice to be able to do at
all...........
Thanks for any feedback
Nancy Schmitt
Histology Coordinator
Dubuque, IA
NOTICE: This email may contain legally privileged information. The information
is for the use of only the intended recipient(s) even if addressed
incorrectly. If you are not the intended recipient, please notify the sender
that you have received it in error and then delete it along with any
attachments. Thank you.
------------------------------
Message: 18
Date: Tue, 13 May 2008 11:30:13 -0500
From: "Michele Wich" <mwich <@t> 7thwavelabs.com>
Subject: [Histonet] Apipophilin and MCM
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<62A8156F8071C8439080D626DF8C33A602E3F5 <@t> wave-mail.7thwave.local>
Content-Type: text/plain; charset="US-ASCII"
Does anyone know if Apipophilin and MCM (mini chromosome maintenance
protein) are commercially available and, if so, who sells them?
Thanks for any info!
This communication is intended solely for the use of the addressee and may
contain information that is legally privileged, confidential or exempt from
disclosure. If you are not the intended recipient, please note that any
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------------------------------
Message: 19
Date: Tue, 13 May 2008 11:35:58 -0500
From: Atoska Gentry <gentras <@t> vetmed.auburn.edu>
Subject: [Histonet] rubber stoppers & tubing
To: Histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <4829C36E.8000504 <@t> vetmed.auburn.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
hello, if any of you have a source for two-hole black rubber stoppers
measuring : 21mm top x 13mm bottom x 7mm in length ( with the
inscription 304w SET on top, and with one hole measuring 2-3mm and the
other 3-4 mm); and or 15mm bottom x 8mm in length; also, rigid plastic
tubing 2-3 mm in diameter, 196mm in length will you please contact me
ASAP? Thank you kindly, Atoska
--
Atoska S. Gentry, B.S., HT(ASCP)
Research Assistant IV
Scott-Ritchey RSCH Center
College of Vet. Med
Auburn, AL 36849
PH (334) 844-5579
FAX (334) 844-5850
email: gentras <@t> vetmed.auburn.edu
------------------------------
Message: 20
Date: Tue, 13 May 2008 10:38:18 -0600
From: "Liz Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] Standardized Microtomes
To: "Nancy Schmitt" <nancy_schmitt <@t> pa-ucl.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EE33BE5C905A3046A7FF8F58A64C8E4B0800E7 <@t> server.PremierLab.local>
Content-Type: text/plain; charset="US-ASCII"
Newcomer supply has a device that will standardize microtomes.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 682-3949
fax (303) 682-9060
liz <@t> premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive
Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nancy
Schmitt
Sent: Tuesday, May 13, 2008 10:28 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Standardized Microtomes
Good Morning to all
Does anybody standardize microtomes so they all cut at same angle? Is
this impossible? We only cut control blocks on one microtome because
they are each at a little different angle. Would be nice to be able to
do at all...........
Thanks for any feedback
Nancy Schmitt
Histology Coordinator
Dubuque, IA
NOTICE: This email may contain legally privileged information. The
information is for the use of only the intended recipient(s) even if
addressed incorrectly. If you are not the intended recipient, please
notify the sender that you have received it in error and then delete it
along with any attachments. Thank you.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 21
Date: Tue, 13 May 2008 11:41:10 -0500
From: Jackie M O'Connor <Jackie.O'Connor <@t> abbott.com>
Subject: Re: [Histonet] Standardized Microtomes
To: Nancy Schmitt <nancy_schmitt <@t> pa-ucl.com>
Cc: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OFF296CF78.F10FFF75-ON86257448.005B93DD-86257448.005BADC0 <@t> abbott.com>
Content-Type: text/plain; charset="US-ASCII"
Get a block aligner. We have aligned all of our microtomes so that any
technicican can do a recut no matter what machine it was originally cut
on.
Nancy Schmitt <nancy_schmitt <@t> pa-ucl.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
05/13/2008 11:28 AM
To
"'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] Standardized Microtomes
Good Morning to all
Does anybody standardize microtomes so they all cut at same angle? Is
this
impossible? We only cut control blocks on one microtome because they are
each at a little different angle. Would be nice to be able to do at
all...........
Thanks for any feedback
Nancy Schmitt
Histology Coordinator
Dubuque, IA
NOTICE: This email may contain legally privileged information. The
information
is for the use of only the intended recipient(s) even if addressed
incorrectly. If you are not the intended recipient, please notify the
sender
that you have received it in error and then delete it along with any
attachments. Thank you.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 22
Date: Tue, 13 May 2008 11:54:13 -0500
From: amy rizzo <arizzo <@t> mcw.edu>
Subject: [Histonet] Paraffin thickness standard or measurement?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c8b519$f8d86440$c91e3086 <@t> marqnet.mu.edu>
Content-Type: text/plain; charset="us-ascii"
Is there anyway to measure the thickness of your paraffin section as it
comes off a microtome? I have noticed mine isn't as consistent as I would
like. Do they sell something anywhere that does this? Thanks,
Amy Rizzo
------------------------------
Message: 23
Date: Tue, 13 May 2008 12:56:55 -0400
From: "Joyce Cline" <jcline <@t> wchsys.org>
Subject: RE: [Histonet] Standardized Microtomes
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001201c8b51a$5996a080$1d2a14ac <@t> wchsys.org>
Content-Type: text/plain; charset="us-ascii"
Each week we align all our microtomes. A company called Advance
Innovations has created an aligner that is available through several
companies. Aligning our microtomes prevents losing tissue from small
biopsies when a recut is called for.
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Good Morning to all
Does anybody standardize microtomes so they all cut at same angle? Is
this
impossible? We only cut control blocks on one microtome because they
are
each at a little different angle. Would be nice to be able to do at
all...........
Thanks for any feedback
Nancy Schmitt
Histology Coordinator
Dubuque, IA
NOTICE: This email may contain legally privileged information. The
information
is for the use of only the intended recipient(s) even if addressed
incorrectly. If you are not the intended recipient, please notify the
sender
that you have received it in error and then delete it along with any
attachments. Thank you.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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End of Histonet Digest, Vol 54, Issue 17
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