[Histonet] Bone Marrow Staining

[Rene J Buesa]

The "washed out" appearance most likely has nothing to do with fixation. Check if you are heating the sections BEFORE they are completely drained off. That can cause that artifact.
  The only thing you have to be aware of when using NBF to fix BM specimens is to control the pH on the staining solutions (specially the Giemsa).
  René J.

Karla Arrington <freckles9660 <@t> yahoo.com> wrote:
  I currently use 10% NBF for fixing bone marrows (core and clot). Although lately
the sections appear "washed out". I previously used B-5 Fixative. Is there a better
fixative for fixing bone marrows so their cells are crisp and clear? 

Karla Arrington
freckles9660 <@t> yahoo.com



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