[Histonet] Hematoxylin too light

[Monfils, Paul]

I don't know what Hematoxylin 7211 is, but a good quality Gill III formula applied for 5 minutes and blued in lithium carbonate solution will produce nuclei about as dark as you can get them with an aluminum-mordanted hematoxylin.

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Paula Lucas
> Reply To: 	Paula Lucas
> Sent: 	Friday, May 9, 2008 11:39 AM
> To: 	histonet <@t> pathology.swmed.edu
> Subject: 	[Histonet] Hematoxylin too light
> 
> Hello,
> 
> My pathologist here likes the nuclear staining very dark.
> 
> Does anyone have such a pathologist who likes a dark nucleus and if so, would you mind suggesting the H&E protocol and brand of hematoxylin used?  
> 
> We have the Leica autostainer and I'm using the Richard Allan 7211 with the times of 2.5 minutes in the Hematoxylin 7211 and 1.5 minutes in Eosin.  I use the Clarifier 1 for 30 seconds and the Bluing reagent for 1 minute.  I increased the time to 5 minutes in the 7211, but it really didn't make any changes. I've been using this method for the past several years, but this past year, the pathologist has been complaining about the stain being too pale.
> 
> I would appreciate any suggestions.  Thanks.
> Paula Lucas
> Lab Manager
> Bio-Path Medical Group 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>