[Histonet] ERRATUM in Why use Calcium Chloride in Trypsin Antigen Retrieval?

[Merced Leiker]

Sorry I meant FORMaldehyde-fixed, not paraformaldehyde....

--On Thursday, May 08, 2008 3:14 PM -0400 Merced Leiker 
<leiker <@t> buffalo.edu> wrote:

> Does anyone know why CaCl2 is used in trypsin buffers for retrieval of
> antigen in paraformaldehyde-fixed, paraffin-embedded tissue sections?  Is
> it really necessary?  Can I just use a Trypsin-EDTA solution we already
> have in the lab?
>
> Merced M Leiker
> Research Technician II
> 354 BRB (Lee Lab) / 140 Farber Hall (mail)
> School of Medicine and Biomedical Sciences
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214
> Ph: (716) 829-6033
> Fx: (716) 829-2725
>
>
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Merced M Leiker
Research Technician II
354 BRB (Lee Lab) / 140 Farber Hall (mail)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725