[Histonet] Polymer IHC Detection

[Gayle Callis]

Amos,

Biocare has more than one species, including goat and are single links, 
thankfully.  Their rodent kit is for either rat or mouse.   The kits can 
also be for either HRP or Alk Phos.  We did tweak their protocol to fit our 
needs using a Alk Phos rabbit kit by eliminating a peroxidase blocking step 
they had in the AP protocol -  since peroxidase was not in the staining loop 
anyway.  Our staining went very well.

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT 59715


----- Original Message ----- 
From: "Amos Brooks" <amosbrooks <@t> gmail.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, May 07, 2008 6:27 PM
Subject: [Histonet] Polymer IHC Detection


> Hi,
>    Does anyone know of a good polymer immunohistochemical detection kit
> that labels antibodise raised in animals other than mouse or rabbit? I am
> looking for a goat or rat detection kit. I know I could use a mouse anti
> goat then a mouse secondary, but I'd like to simplify the process. Of 
> course
> vendor responses are more than welcome.
>
> Thanks,
> Amos Brooks
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