[Histonet] cryoprotection

[John Kiernan]

   Transfer  the  brain,  after  adequate fixation in buffered
   forma   brain  sin   cutting  sections.   should  still  freeze  as  q   on a  metal  cryostat  chuck,   compound  for  adhesion, then stand the chu   CO2  and  either acetone or alcohol. Mount the c   in   the   cryostat   and   wait  for  its  temperature    equilibrate. This isn't the fastest way to freeze thing    but  it's  good  enough  for cryoprotected formaldehyde-fixed rodent
   brain   UWO<BR>   <BR>----- Or   &lt;tshrobertso   May    5,    2008    12:41<   cryoprotection<BR>To: histonet <@t> li   sts.utsouthwestern.edu<BR><BR>&gt;   i  am  trying  to
   cryopr   <BR>&gt;   brain  in   sucrose. can you please <BR>   to     prevent     holes    in    my    sample?<BR   &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;   <BR>&gt;  ---------------------------------<BR>&gt; Be
   a   <BR>&gt   ______   _______________________   5F   _________________<BR>&gt;
   Hist   Histonet <@t> lists.utsouthwestern.ed   http://lists.utsouthwestern.edu/mailman/listinfo