[Histonet] Looking for detailed protocol to stain for PAS in liver sections (paraffin)

[Tony Henwood]

Use a section of appendix or other GIT tract. Mucin in the goblet cells
should stain.
Also look at your liver sections. Are basement membranes staining?

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Igor
Nasonkin
Sent: Wednesday, 7 May 2008 9:24 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Looking for detailed protocol to stain for PAS in
liver sections (paraffin)


Hi everybody,

I am looking for a detailed protocol for PAS staining in liver sections
(paraffin-embedded). The 1st set of sections did not work, and we had no
+control since we have not done it. These are 4-6wk mouse liver sections
embedded in paraffin; our lab is experienced in IHC on paraffin sections
so these were done right. But PAS staining did not work. What could be
main reasons? Any positive control we could use? Thank you in advance,
Igor


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