[Histonet] RINSING IN TAP WATER AFTER DECAL

[Rena Fail]

The rinse step is necessary between formalin and RDO, the two chemicals
should not be combined
Rena

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Fischer,
R. B
Sent: Tuesday, May 06, 2008 12:22 PM
To: histonet <@t> lists.utsouthwestern.edu
Cc: Delcambre, Linda V
Subject: [Histonet] RINSING IN TAP WATER AFTER DECAL

We have been using "RDO" and "CAL-X" decal solutions for some time now.
After the specified times in the decal solutions, the specimens are
rinsed in running tap water for about 10 minutes before being placed
back in 10%NBF for routine processing. We were rinsing to get rid of the
nitric acid. My questions are (1) Is this rinse step necessary? If it
is, (2) How long is an adequate time for rinsing?(3) How are other labs
handling the specimen post decal, and before processing?
 
 
 
R.Brian Fischer
Histology Lead Tech
Community Hospital of the Monterey Peninsula
PO Box HH Monterey Ca. 93942
831-625-4791
Fax: 831-6583683
 
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