[Histonet] Re: RINSING IN TAP WATER AFTER DECAL

[Eric Mahoney]

I have used Cal-EX by Fisher before.  After decal. I ran my samples in
running tap water for 4 hours.  I am decal. vertebral bone and my
sections and HE staining came out pretty well.  I would not skip this
step. 

>>> <histonet-request <@t> lists.utsouthwestern.edu> 05/06/08 1:02 PM >>>
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Today's Topics:

   1. RE: metal mesh gloves (Jerry Helisek)
   2. Glenda Hood Please Call Me (Pamela Marcum)
   3. (no subject) (Pamela Marcum)
   4. RE: RE: metal mesh gloves (Bonner, Janet)
   5. RE: RE: metal mesh gloves (Dave Johnson)
   6. Cryostat to donate (Weems, Joyce)
   7. California labs-have you moved recently? (Barbara Albert)
   8. RE: (no subject) (Tony Henwood)
   9. B-plus question (karen adams)
  10. Re: B-plus question (Rene J Buesa)
  11. Re: B-plus question (Paul Bradbury)
  12. GLP question (Linke_Noelle)
  13. Proper Grossing Attire (Mike Pence)
  14. pan cytokeratin markers (Colin Nixon)
  15. Goodbye and Thanks (Douglas D Deltour)
  16. RINSING IN TAP WATER AFTER DECAL (Fischer, R. B)
  17. gross-section (Galina Deyneko)


----------------------------------------------------------------------

Message: 1
Date: Mon, 5 May 2008 13:13:35 -0400
From: "Jerry Helisek" <Jerry <@t> ralambusa.com>
Subject: [Histonet] RE: metal mesh gloves
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<3855F92002259948A66A8CA2D16E3A4F05B029 <@t> server.ralambusa.com>
Content-Type: text/plain;	charset="us-ascii"

Helen,

I have a restaurant here in Durham, NC and we purchase mesh gloves at
our local Restaurant Supply Store, just do a google search or next time
you go into a "local" restaurant ask the owner/manager (the only reason
I emphasize "local" is that, "local" proprietors are more likely to
purchase locally rather than shop from their larger parent companies
designated suppliers...).

They are a must for cleaning/assembly of sharp equipment.

Good luck!

------------------------------------

Raymond A. Lamb, Inc

Jerry Helisek

VP North America

jerry <@t> ralambusa.com

5409 Lumley Road, Unit 102

Durham, North Carolina 27703

tel: 919.957.1964

fax: 919.957.1972

mobile: 919.264.7964

Skype ID:jerryhelisek

------------------------------------


------------------------------

Message: 5
Date: Mon, 5 May 2008 10:12:25 -0400
From: Helen E Johnson <hej01 <@t> health.state.ny.us>
Subject: [Histonet] metal mesh gloves
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<mailman.0.1210006800.26151.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset=US-ASCII


Hi Histonetters,
     Can you please recommend a vendor that sells a good pair of metal
mesh
gloves.
                                      Thanks.             Helen Johnson
(hej01 <@t> health.state.ny.us)


IMPORTANT NOTICE:  This e-mail and any attachments may contain
confidential or sensitive information which is, or may be, legally
privileged or otherwise protected by law from further disclosure.  It is
intended only for the addressee.  If you received this in error or from
someone who was not authorized to send it to you, please do not
distribute, copy or use it or any attachments.  Please notify the sender
immediately by reply e-mail and delete this from your system. Thank you
for your cooperation.




------------------------------

Message: 2
Date: Mon, 05 May 2008 13:55:57 -0400
From: Pamela Marcum <pmarcum <@t> vet.upenn.edu>
Subject: [Histonet] Glenda Hood Please Call Me
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <6.2.5.6.2.20080505135440.01c97170 <@t> vet.upe

Hi All,

I am sorry but I need to contact Glenda Hood or have her contact 
me.  We have a microtome for her project.


Best Regards,

Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr.  CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348

Phone - 610-925-6278
Fax     - 610-925-8120
E-mail - pmarcum <@t> vet.upenn.edu 




------------------------------

Message: 3
Date: Mon, 05 May 2008 13:56:02 -0400
From: Pamela Marcum <pmarcum <@t> vet.upenn.edu>
Subject: [Histonet] (no subject)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <6.2.5.6.2.20080505135433.01c859c0 <@t> vet.upenn.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed



Best Regards,

Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr.  CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348

Phone - 610-925-6278
Fax     - 610-925-8120
E-mail - pmarcum <@t> vet.upenn.edu 




------------------------------

Message: 4
Date: Mon, 5 May 2008 14:11:44 -0400
From: "Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>
Subject: RE: [Histonet] RE: metal mesh gloves
To: "Jerry Helisek" <Jerry <@t> ralambusa.com>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<5F31F38C96781A4FBE3196EBC22D47807F260E <@t> fhosxchmb006.ADVENTISTCORP.NET>
	
Content-Type: text/plain; charset=iso-8859-1

OR...Lab Safety Supply.

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Jerry
Helisek
Sent: Mon 5/5/2008 1:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: metal mesh gloves



Helen,

I have a restaurant here in Durham, NC and we purchase mesh gloves at
our local Restaurant Supply Store, just do a google search or next time
you go into a "local" restaurant ask the owner/manager (the only reason
I emphasize "local" is that, "local" proprietors are more likely to
purchase locally rather than shop from their larger parent companies
designated suppliers...).

They are a must for cleaning/assembly of sharp equipment.

Good luck!

------------------------------------

Raymond A. Lamb, Inc

Jerry Helisek

VP North America

jerry <@t> ralambusa.com

5409 Lumley Road, Unit 102

Durham, North Carolina 27703

tel: 919.957.1964

fax: 919.957.1972

mobile: 919.264.7964

Skype ID:jerryhelisek

------------------------------------


------------------------------

Message: 5
Date: Mon, 5 May 2008 10:12:25 -0400
From: Helen E Johnson <hej01 <@t> health.state.ny.us>
Subject: [Histonet] metal mesh gloves
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <mailman.0.1210006800.26151.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset=US-ASCII


Hi Histonetters,
     Can you please recommend a vendor that sells a good pair of metal
mesh
gloves.
                                      Thanks.             Helen Johnson
(hej01 <@t> health.state.ny.us)


IMPORTANT NOTICE:  This e-mail and any attachments may contain
confidential or sensitive information which is, or may be, legally
privileged or otherwise protected by law from further disclosure.  It is
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someone who was not authorized to send it to you, please do not
distribute, copy or use it or any attachments.  Please notify the sender
immediately by reply e-mail and delete this from your system. Thank you
for your cooperation.


_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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------------------------------

Message: 5
Date: Mon, 5 May 2008 14:17:04 -0400
From: "Dave Johnson" <djohnson <@t> mercedesmedical.com>
Subject: RE: [Histonet] RE: metal mesh gloves
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F7CEA5A181F69F4C84A352BD311BCEBD7A0017 <@t> mail1.mercedesmedical.com>
Content-Type: text/plain;	charset="us-ascii"

We carry them

Dave Johnson
Mercedes Medical 
800-331-2716

 
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bonner,
Janet
Sent: Monday, May 05, 2008 2:12 PM
To: Jerry Helisek; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: metal mesh gloves


OR...Lab Safety Supply.


From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Jerry
Helisek
Sent: Mon 5/5/2008 1:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: metal mesh gloves


I have a restaurant here in Durham, NC and we purchase mesh gloves at
our local Restaurant Supply Store, just do a google search or next time
you go into a "local" restaurant ask the owner/manager (the only reason
I emphasize "local" is that, "local" proprietors are more likely to
purchase locally rather than shop from their larger parent companies
designated suppliers...).

They are a must for cleaning/assembly of sharp equipment.

Raymond A. Lamb, Inc
Jerry Helisek
VP North America
jerry <@t> ralambusa.com
5409 Lumley Road, Unit 102
Durham, North Carolina 27703
tel: 919.957.1964



Date: Mon, 5 May 2008 10:12:25 -0400
From: Helen E Johnson <hej01 <@t> health.state.ny.us>
Subject: [Histonet] metal mesh gloves
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <mailman.0.1210006800.26151.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset=US-ASCII


Hi Histonetters,
     Can you please recommend a vendor that sells a good pair of metal
mesh gloves.
                                      Thanks.             Helen Johnson
(hej01 <@t> health.state.ny.us) 



------------------------------

Message: 6
Date: Mon, 5 May 2008 14:40:47 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: [Histonet] Cryostat to donate
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<982A0A9461F9BF438C7B19A6E425A38312AA87 <@t> ITSSSXM01V6.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"

Does anyone have a working cryostat that they could donate to Doctors
Without Borders? I don't know details, but will find out if anyone could
help.
 
Thanks and happy Monday! j
 
Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax
 
Confidentiality Notice:
This email, including any attachments is the 
property of Catholic Health East and is intended 
for the sole use of the intended recipient(s).  
It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are 
not the intended recipient, please reply to the 
sender that you have received the message in 
error, then delete this message.


------------------------------

Message: 7
Date: Mon, 5 May 2008 14:45:37 -0700 (PDT)
From: Barbara Albert <barbaraaalbert <@t> yahoo.com>
Subject: [Histonet] California labs-have you moved recently?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <707321.90101.qm <@t> web63711.mail.re1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi all,
  We moving soon and would sincerely appreciate any tips or warnings of
any pitfalls.  We are partiularly interestes in the procedure used to
validate your equipment.
   
  Thanks,
  Barbara Albert
  UCSF Medical Center
  San Francisco

       
-------------------------------------------------------------

Message: 8
Date: Tue, 6 May 2008 09:25:33 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] (no subject)
To: "Pamela Marcum" <pmarcum <@t> vet.upenn.edu>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555202FAFEA8 <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"

Thanks!!

??

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pamela
Marcum
Sent: Tuesday, 6 May 2008 3:56 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] (no subject)




Best Regards,

Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr.  CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348

Phone - 610-925-6278
Fax     - 610-925-8120
E-mail - pmarcum <@t> vet.upenn.edu 


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------------------------------

Message: 9
Date: Tue, 6 May 2008 09:00:54 -0400
From: "karen adams" <kadamsplw <@t> gmail.com>
Subject: [Histonet] B-plus question
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<acf585930805060600u7552d5eeo9680eb0aab94cc4b <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hello all....we are changing bone marrow fixative from formalin to
B-plus.
If we fix for the required time in B-plu and then process on the VIP w/
the
other specimens using formalin are there any effects on the tissue going
from B-plus to formalin??
Thank you in advance
-- 
Karen Adams
Supervisor
Pathology Laboratories West
9303 Park West Blvd.
Knoxville, TN 37923
kadamsplw <@t> gmail.com


------------------------------

Message: 10
Date: Tue, 6 May 2008 06:15:30 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] B-plus question
To: karen adams <kadamsplw <@t> gmail.com>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <621028.53193.qm <@t> web65713.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

No, because the tissue is supposed to be fixed already.
  RenΘ J.

karen adams <kadamsplw <@t> gmail.com> wrote:
  Hello all....we are changing bone marrow fixative from formalin to
B-plus.
If we fix for the required time in B-plu and then process on the VIP w/
the
other specimens using formalin are there any effects on the tissue going
from B-plus to formalin??
Thank you in advance
-- 
Karen Adams
Supervisor
Pathology Laboratories West
9303 Park West Blvd.
Knoxville, TN 37923
kadamsplw <@t> gmail.com
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Message: 11
Date: Tue, 06 May 2008 06:52:32 -0700
FromTo: karen adams <kadamsplw <@t> gmail.com>,	HistoNet Server
	<histonet <@t> pathology.swmed.edu>
Message-ID: <482062A0.4080500 <@t> shaw.ca>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Hi Karen,
 I have been using the same sequence of reagents for several years with 
great success. We routinely fix bone marrow cores for 3 hours in B-plus,

rinse in water, decalcify, rinse again, and put the cassette in with all

the other tissues for processing. B-plus contains formaldehyde anyway, 
so your are not introducing a different reagent when you transfer them 
to formalin during processing.

B-plus gives much better cytological detail than formalin. Nuclear 
detail is crisper, granules are better preserved, hemosiderin is not 
effected. CD-3, CD-20, kappa and lambda, etc. all work beautifully after

B-plus. It also has the advantage of not producing an fixation artefact 
pigment like B-5 does. I think you will be happy with your decision to 
change.

Paul Bradbury
Kamloops, Canada


karen adams wrote:
> Hello all....we are changing bone marrow fixative from formalin to
B-plus.
> If we fix for the required time in B-plu and then process on the VIP
w/ the
> other specimens using formalin are there any effects on the tissue
going
> from B-plus to formalin??
> Thank you in advance
>   





------------------------------

Message: 12
Date: Tue, 6 May 2008 07:24:37 -0700
From: "Linke_Noelle" <Linke_Noelle <@t> Allergan.com>
Subject: [Histonet] GLP question
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<5C3DA4BE34AA0641BAA10A7C1478B60527D204 <@t> IRMAIL132.irvine.allergan.com>
Content-Type: text/plain;	charset="iso-8859-1"

Hi everyone,

I have a question for all of the GLP compliant folks out there.  How do
you handle recuts that you do prior to giving slides to the pathologist?
 For example, you stain on Monday, look at your slides and find you are
missing something like the mammary gland.  You recut and stain on
Tuesday....how do you document that?  Do you keep your original slide
and archive it?  

I thank you in advance for any help!

Noelle

Noδlle Linke, MS, HTL(ASCP)QIHC
Allergan, Inc
2525 Dupont Drive RD-2A
Irvine, CA 92612
714-246-5568



------------------------------

Message: 13
Date: Tue, 6 May 2008 09:39:17 -0500
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: [Histonet] Proper Grossing Attire
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<661949901A768E4F9CC16D8AF8F2838C017A3816 <@t> IS-E2K3.grhs.net>
Content-Type: text/plain;	charset="us-ascii"

I would like to hear everyone's opinions on what the proper PPE is for
"grossing in" specimens.  Two levels of grossing in are: 1) dictating
and submit specimen - limited to bisecting a punch bx and 2) dictating,
describing, cutting, taking selected sections - some judgment and
advanced training required ie. colons-placentas.
 
My question is: "Is gloves alone appropriate attire for the first level?
What do you wear when grossing at the second level?
 
You may choose to reply off line as to save yourself much "flaming" I
sure.
 
Thanks in advance,
Mike


------------------------------

Message: 14
Date: Tue, 6 May 2008 16:13:32 +0100
From: "Colin Nixon" <c.nixon <@t> beatson.gla.ac.uk>
Subject: [Histonet] pan cytokeratin markers
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<0E5DFBD0E5E27443A0987F7664787163018D2B10 <@t> exchange-be4.centre.ad.gla.ac.uk>
	
Content-Type: text/plain;	charset="iso-8859-1"

Can anyone recommend a good pan cytokeratin antibody for use on mouse
tissue for paraffin fixed embedded immunohistochemistry and if they can
what retrieval method/dilution etc that they use?
 
Thanks,
 
Colin 


------------------------------

Message: 15
Date: Tue, 6 May 2008 11:30:39 -0500
From: "Douglas D Deltour" <doug <@t> ppspath.com>
Subject: [Histonet] Goodbye and Thanks
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<mailman.0.1210093200.11428.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain;	charset="US-ASCII"

I wanted to thank all that participate on histonet and for making this
such
a valuable asset. I have enjoyed it very much. I will be departing my
current position on May 23rd. I will be taking a position with an IHC
company (technical side) that will have me in the sun of South Florida.
I
will be reduced to lurking histonet when I have the chance. Keep up the
great work and thanks for all that you do. 

 

 

Douglas D. Deltour HT(ASCP)

Histology Manager

Professional Pathology Services, PC

One Science Court

Suite 200

Columbia, SC 29203

Office (803)252-1913

Fax (803)254-3262

Doug <@t> ppspath.com 

*****************************************************

PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the
reader
of this message is not the intended recipient, you are hereby notified
that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.

 



------------------------------

Message: 16
Date: Tue, 6 May 2008 09:21:54 -0700
From: "Fischer, R. B" <BFicher <@t> chomp.org>
Subject: [Histonet] RINSING IN TAP WATER AFTER DECAL
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: "Delcambre, Linda V" <Linda.Delcambre <@t> chomp.org>
Message-ID:
	<AD7D9D582805F740B0D79FB0EB8EDA6403B02E3D <@t> EXCHANGE.chomp.org>
Content-Type: text/plain;	charset="us-ascii"

We have been using "RDO" and "CAL-X" decal solutions for some time now.
After the specified times in the decal solutions, the specimens are
rinsed in running tap water for about 10 minutes before being placed
back in 10%NBF for routine processing. We were rinsing to get rid of the
nitric acid. My questions are (1) Is this rinse step necessary? If it
is, (2) How long is an adequate time for rinsing?(3) How are other labs
handling the specimen post decal, and before processing?
 
 
 
R.Brian Fischer
Histology Lead Tech
Community Hospital of the Monterey Peninsula
PO Box HH Monterey Ca. 93942
831-625-4791
Fax: 831-6583683
 
Confidentiality Notice:
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Peninsula.  This message and any attached documents may be confidential
and contain information protected by state and federal medical privacy
statutes.  They are intended only for the use of the addressee.  If you
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of this information is strictly prohibited.  If you received this
transmission in error, please accept our apologies and notify the
sender.

Thank you.




------------------------------

Message: 17
Date: Tue, 6 May 2008 09:28:19 -0700 (PDT)
From: Galina Deyneko <galinadeyneko <@t> yahoo.com>
Subject: [Histonet] gross-section
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <397885.31237.qm <@t> web33101.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1



Dear Colleagues
  Could you please advise the sources like manuals, textbooks or
websites for gross-sectioning/ sampling of the organs of big mammalians
( we work with rabbits, monkeys). I think human gross-sectioning
information also useful.
  Thank you.
  galina deyneko
  novartis,cambridge, ma
   

       
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