[Histonet] Paraffin types

[Piero Nelva]

I've only ever used the same wax for both processes.  Paraplast is the 
current (>10 years) favorite.

Piero


----- Original Message ----- 
From: "Anne van Binsbergen" <annigyg <@t> gmail.com>
To: "Gayle Callis" <gayle.callis <@t> bresnan.net>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, May 02, 2008 4:59 PM
Subject: Re: [Histonet] Paraffin types


> one size fits all - tissue-tek works for me - been using it for over 10
> years now - in all the labs i have managed
> Annie
>
> 2008/5/2 Gayle Callis <gayle.callis <@t> bresnan.net>:
>
>> Same paraffin for infiltration and embedding.  Our choice is especially
>> good for bone because it is a harder paraffin and works well for soft
>> tissues even very thin sections i.g. 1 to 2 um.  Since we do a great deal 
>> of
>> bone work, this has been a good choice.    Tissue Prep 2 from 
>> ThermoFisher
>> Scientific.
>>
>> We have used the Surgipath combination infiltration media followed by
>> their embedding media with great success.
>>
>> Gayle M. Callis
>> HTL/HT/MT(ASCP)
>> Bozeman MT 59715
>>
>>
>> ----- Original Message ----- From: "Joyce Cline" <jcline <@t> wchsys.org>
>> To: <histonet <@t> lists.utsouthwestern.edu>
>> Sent: Thursday, May 01, 2008 3:06 PM
>> Subject: [Histonet] Paraffin types
>>
>>
>>  What type of paraffin is everyone using? Separate paraffin for
>> > processing and embedding, or the same for processing and embedding?
>> >
>> > Paraplast, Surgipath, Shandon ????
>> >
>> >
>> >
>>
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>>
>
>
>
> -- 
> Anne (van Binsbergen) Hope
> Abu Dhabi
> UAE
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